LOCUS       DAJTSA010000001        96256 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000001 DAJTSA010000000
VERSION     DAJTSA010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 96256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 96256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..96256
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.001"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            220..579
                     /locus_tag="OO369_000001"
     CDS             220..579
                     /locus_tag="OO369_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5980436.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            581..973
                     /locus_tag="OO369_000002"
     CDS             581..973
                     /locus_tag="OO369_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5980437.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(1025..2440)
                     /gene="gltX"
                     /locus_tag="OO369_000003"
     CDS             complement(1025..2440)
                     /gene="gltX"
                     /locus_tag="OO369_000003"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR5980438.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            2699..2774
                     /locus_tag="OO369_000004"
     tRNA            2699..2774
                     /locus_tag="OO369_000004"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2732..2734,aa:Val,seq:tac)
     gene            2819..2894
                     /locus_tag="OO369_000005"
     tRNA            2819..2894
                     /locus_tag="OO369_000005"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2852..2854,aa:Val,seq:tac)
     gene            2899..2974
                     /locus_tag="OO369_000006"
     tRNA            2899..2974
                     /locus_tag="OO369_000006"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2932..2934,aa:Lys,seq:ttt)
     gene            3005..3130
                     /locus_tag="OO369_000007"
     ncRNA           3005..3130
                     /ncRNA_class="other"
                     /locus_tag="OO369_000007"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            3250..3591
                     /locus_tag="OO369_000008"
     CDS             3250..3591
                     /locus_tag="OO369_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR5980439.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(3582..4508)
                     /locus_tag="OO369_000009"
     CDS             complement(3582..4508)
                     /locus_tag="OO369_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5980440.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            4598..5596
                     /locus_tag="OO369_000010"
     CDS             4598..5596
                     /locus_tag="OO369_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR5980441.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(5593..5811)
                     /locus_tag="OO369_000011"
     CDS             complement(5593..5811)
                     /locus_tag="OO369_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR5980442.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(5813..7828)
                     /gene="ligA"
                     /locus_tag="OO369_000012"
     CDS             complement(5813..7828)
                     /gene="ligA"
                     /locus_tag="OO369_000012"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR5980443.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(7899..8885)
                     /gene="zipA"
                     /locus_tag="OO369_000013"
     CDS             complement(7899..8885)
                     /gene="zipA"
                     /locus_tag="OO369_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR5980444.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            9115..9876
                     /gene="cysZ"
                     /locus_tag="OO369_000014"
     CDS             9115..9876
                     /gene="cysZ"
                     /locus_tag="OO369_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR5980445.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            10061..11032
                     /gene="cysK"
                     /locus_tag="OO369_000015"
     CDS             10061..11032
                     /gene="cysK"
                     /locus_tag="OO369_000015"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR5980446.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            11416..11673
                     /gene="ptsH"
                     /locus_tag="OO369_000016"
     CDS             11416..11673
                     /gene="ptsH"
                     /locus_tag="OO369_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR5980447.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            11718..13445
                     /gene="ptsI"
                     /locus_tag="OO369_000017"
     CDS             11718..13445
                     /gene="ptsI"
                     /locus_tag="OO369_000017"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR5980448.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            13486..13995
                     /gene="crr"
                     /locus_tag="OO369_000018"
     CDS             13486..13995
                     /gene="crr"
                     /locus_tag="OO369_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR5980449.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(14037..14888)
                     /gene="pdxK"
                     /locus_tag="OO369_000019"
                     /gene_synonym="thiJ"
     CDS             complement(14037..14888)
                     /gene="pdxK"
                     /locus_tag="OO369_000019"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR5980450.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            14993..15367
                     /locus_tag="OO369_000020"
     CDS             14993..15367
                     /locus_tag="OO369_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR5980451.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            15400..16123
                     /locus_tag="OO369_000021"
                     /pseudo
     CDS             15400..16123
                     /locus_tag="OO369_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(16327..17238)
                     /gene="cysM"
                     /locus_tag="OO369_000022"
     CDS             complement(16327..17238)
                     /gene="cysM"
                     /locus_tag="OO369_000022"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR5980452.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(17372..18469)
                     /gene="cysA"
                     /locus_tag="OO369_000023"
     CDS             complement(17372..18469)
                     /gene="cysA"
                     /locus_tag="OO369_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR5980453.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(18459..19334)
                     /gene="cysW"
                     /locus_tag="OO369_000024"
     CDS             complement(18459..19334)
                     /gene="cysW"
                     /locus_tag="OO369_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR5980454.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(19334..20167)
                     /gene="cysT"
                     /locus_tag="OO369_000025"
     CDS             complement(19334..20167)
                     /gene="cysT"
                     /locus_tag="OO369_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR5980455.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(20167..21183)
                     /gene="cysP"
                     /locus_tag="OO369_000026"
     CDS             complement(20167..21183)
                     /gene="cysP"
                     /locus_tag="OO369_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR5980456.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(21487..22278)
                     /gene="ucpA"
                     /locus_tag="OO369_000027"
     CDS             complement(21487..22278)
                     /gene="ucpA"
                     /locus_tag="OO369_000027"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR5980457.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(22465..23265)
                     /gene="murR"
                     /locus_tag="OO369_000028"
     CDS             complement(22465..23265)
                     /gene="murR"
                     /locus_tag="OO369_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR5980458.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            23429..24325
                     /gene="murQ"
                     /locus_tag="OO369_000029"
     CDS             23429..24325
                     /gene="murQ"
                     /locus_tag="OO369_000029"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR5980459.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            24329..25753
                     /gene="murP"
                     /locus_tag="OO369_000030"
     CDS             24329..25753
                     /gene="murP"
                     /locus_tag="OO369_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR5980460.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(25933..26832)
                     /gene="yfeX"
                     /locus_tag="OO369_000031"
     CDS             complement(25933..26832)
                     /gene="yfeX"
                     /locus_tag="OO369_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR5980461.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(26928..27503)
                     /locus_tag="OO369_000032"
     CDS             complement(26928..27503)
                     /locus_tag="OO369_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR5980462.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(27564..28013)
                     /locus_tag="OO369_000033"
     CDS             complement(27564..28013)
                     /locus_tag="OO369_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR5980463.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(28000..28425)
                     /locus_tag="OO369_000034"
     CDS             complement(28000..28425)
                     /locus_tag="OO369_000034"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5980464.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            28639..29508
                     /gene="amiA"
                     /locus_tag="OO369_000035"
     CDS             28639..29508
                     /gene="amiA"
                     /locus_tag="OO369_000035"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR5980465.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            29512..30411
                     /gene="hemF"
                     /locus_tag="OO369_000036"
     CDS             29512..30411
                     /gene="hemF"
                     /locus_tag="OO369_000036"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR5980466.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(30417..31469)
                     /gene="eutR"
                     /locus_tag="OO369_000037"
                     /pseudo
     CDS             complement(30417..31469)
                     /gene="eutR"
                     /locus_tag="OO369_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(31515..32015)
                     /gene="eutK"
                     /locus_tag="OO369_000038"
     CDS             complement(31515..32015)
                     /gene="eutK"
                     /locus_tag="OO369_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR5980467.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(32028..32687)
                     /gene="eutL"
                     /locus_tag="OO369_000039"
     CDS             complement(32028..32687)
                     /gene="eutL"
                     /locus_tag="OO369_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR5980468.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            33869..34660
                     /locus_tag="OO369_000040"
     CDS             33869..34660
                     /locus_tag="OO369_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5980469.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            35380..35775
                     /locus_tag="OO369_000041"
     CDS             35380..35775
                     /locus_tag="OO369_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5980470.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            35793..36056
                     /locus_tag="OO369_000042"
     CDS             35793..36056
                     /locus_tag="OO369_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5980471.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            36079..36621
                     /locus_tag="OO369_000043"
     CDS             36079..36621
                     /locus_tag="OO369_000043"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5980472.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            36760..37047
                     /locus_tag="OO369_000044"
     CDS             36760..37047
                     /locus_tag="OO369_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5980473.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            37086..37292
                     /locus_tag="OO369_000045"
     CDS             37086..37292
                     /locus_tag="OO369_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5980474.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            37604..38089
                     /locus_tag="OO369_000046"
     CDS             37604..38089
                     /locus_tag="OO369_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5980475.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            38070..38348
                     /locus_tag="OO369_000047"
     CDS             38070..38348
                     /locus_tag="OO369_000047"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5980476.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            38341..39801
                     /locus_tag="OO369_000048"
     CDS             38341..39801
                     /locus_tag="OO369_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5980477.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            39835..40644
                     /gene="murI"
                     /locus_tag="OO369_000049"
     CDS             39835..40644
                     /gene="murI"
                     /locus_tag="OO369_000049"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5980478.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(40763..41457)
                     /locus_tag="OO369_000050"
     CDS             complement(join(40763..41211,41211..41457))
                     /locus_tag="OO369_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5980479.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(41976..43349)
                     /gene="eutA"
                     /locus_tag="OO369_000051"
     CDS             complement(41976..43349)
                     /gene="eutA"
                     /locus_tag="OO369_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR5980480.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(43324..43803)
                     /gene="eutP"
                     /locus_tag="OO369_000052"
     CDS             complement(43324..43803)
                     /gene="eutP"
                     /locus_tag="OO369_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR5980481.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(43816..44151)
                     /gene="eutS"
                     /locus_tag="OO369_000053"
     CDS             complement(43816..44151)
                     /gene="eutS"
                     /locus_tag="OO369_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR5980482.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(44444..46723)
                     /gene="maeB"
                     /locus_tag="OO369_000054"
     CDS             complement(44444..46723)
                     /gene="maeB"
                     /locus_tag="OO369_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR5980483.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            47012..47962
                     /gene="tal"
                     /locus_tag="OO369_000055"
     CDS             47012..47962
                     /gene="tal"
                     /locus_tag="OO369_000055"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5980484.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            47982..49985
                     /gene="tkt"
                     /locus_tag="OO369_000056"
     CDS             47982..49985
                     /gene="tkt"
                     /locus_tag="OO369_000056"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5980485.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(50088..51131)
                     /locus_tag="OO369_000057"
     CDS             complement(50088..51131)
                     /locus_tag="OO369_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR5980486.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(51257..51832)
                     /gene="nudK"
                     /locus_tag="OO369_000058"
     CDS             complement(51257..51832)
                     /gene="nudK"
                     /locus_tag="OO369_000058"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR5980487.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(51900..53879)
                     /gene="aegA"
                     /locus_tag="OO369_000059"
     CDS             complement(51900..53879)
                     /gene="aegA"
                     /locus_tag="OO369_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR5980488.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            54085..55802
                     /gene="narQ"
                     /locus_tag="OO369_000060"
                     /pseudo
     CDS             54085..55802
                     /gene="narQ"
                     /locus_tag="OO369_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            56004..59117
                     /gene="acrD"
                     /locus_tag="OO369_000061"
     CDS             56004..59117
                     /gene="acrD"
                     /locus_tag="OO369_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR5980489.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(59216..59275)
                     /gene="ypfM"
                     /locus_tag="OO369_000062"
     CDS             complement(59216..59275)
                     /gene="ypfM"
                     /locus_tag="OO369_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR5980490.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            59656..60012
                     /locus_tag="OO369_000063"
     CDS             59656..60012
                     /locus_tag="OO369_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR5980491.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            60016..61143
                     /gene="dapE"
                     /locus_tag="OO369_000064"
     CDS             60016..61143
                     /gene="dapE"
                     /locus_tag="OO369_000064"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR5980492.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            61171..61371
                     /locus_tag="OO369_000065"
     CDS             61171..61371
                     /locus_tag="OO369_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR5980493.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(61481..62179)
                     /gene="ypfH"
                     /locus_tag="OO369_000066"
     CDS             complement(61481..62179)
                     /gene="ypfH"
                     /locus_tag="OO369_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5980494.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(62253..64268)
                     /gene="tmcA"
                     /locus_tag="OO369_000067"
     CDS             complement(62253..64268)
                     /gene="tmcA"
                     /locus_tag="OO369_000067"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR5980495.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(64283..65146)
                     /locus_tag="OO369_000068"
     CDS             complement(64283..65146)
                     /locus_tag="OO369_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR5980496.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(65314..66027)
                     /gene="purC"
                     /locus_tag="OO369_000069"
     CDS             complement(65314..66027)
                     /gene="purC"
                     /locus_tag="OO369_000069"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR5980497.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(66240..67274)
                     /gene="bamC"
                     /locus_tag="OO369_000070"
     CDS             complement(66240..67274)
                     /gene="bamC"
                     /locus_tag="OO369_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR5980498.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(67291..68169)
                     /gene="dapA"
                     /locus_tag="OO369_000071"
     CDS             complement(67291..68169)
                     /gene="dapA"
                     /locus_tag="OO369_000071"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR5980499.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            68315..68887
                     /locus_tag="OO369_000072"
     CDS             68315..68887
                     /locus_tag="OO369_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR5980500.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            68887..69357
                     /gene="bcp"
                     /locus_tag="OO369_000073"
     CDS             68887..69357
                     /gene="bcp"
                     /locus_tag="OO369_000073"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR5980501.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            69610..70227
                     /gene="hyfA"
                     /locus_tag="OO369_000074"
     CDS             69610..70227
                     /gene="hyfA"
                     /locus_tag="OO369_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR5980502.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            70227..72244
                     /gene="hyfB"
                     /locus_tag="OO369_000075"
                     /pseudo
     CDS             70227..72244
                     /gene="hyfB"
                     /locus_tag="OO369_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            72255..73202
                     /gene="hyfC"
                     /locus_tag="OO369_000076"
     CDS             72255..73202
                     /gene="hyfC"
                     /locus_tag="OO369_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR5980503.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            73219..74658
                     /gene="hyfD"
                     /locus_tag="OO369_000077"
     CDS             73219..74658
                     /gene="hyfD"
                     /locus_tag="OO369_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR5980504.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            74670..75320
                     /gene="hyfE"
                     /locus_tag="OO369_000078"
     CDS             74670..75320
                     /gene="hyfE"
                     /locus_tag="OO369_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR5980505.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            75325..76911
                     /gene="hyfF"
                     /locus_tag="OO369_000079"
                     /pseudo
     CDS             75325..76911
                     /gene="hyfF"
                     /locus_tag="OO369_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
     gene            76901..78615
                     /gene="hyfG"
                     /locus_tag="OO369_000080"
                     /pseudo
     CDS             76901..78615
                     /gene="hyfG"
                     /locus_tag="OO369_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            78625..79170
                     /gene="hyfH"
                     /locus_tag="OO369_000081"
     CDS             78625..79170
                     /gene="hyfH"
                     /locus_tag="OO369_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR5980506.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            79167..79925
                     /gene="hyfI"
                     /locus_tag="OO369_000082"
     CDS             79167..79925
                     /gene="hyfI"
                     /locus_tag="OO369_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR5980507.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            79918..80331
                     /gene="hyfJ"
                     /locus_tag="OO369_000083"
     CDS             79918..80331
                     /gene="hyfJ"
                     /locus_tag="OO369_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR5980508.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            80361..82190
                     /gene="hyfR"
                     /locus_tag="OO369_000084"
     CDS             80361..82190
                     /gene="hyfR"
                     /locus_tag="OO369_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR5980509.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVSKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMA"
     gene            82191..82373
                     /locus_tag="OO369_000085"
     CDS             82191..82373
                     /locus_tag="OO369_000085"
                     /inference="COORDINATES: protein motif:HMM:NF014950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HCR5980510.1"
                     /translation="MLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTT
                     LLSRMQRLGISVREVL"
     gene            82395..83243
                     /locus_tag="OO369_000086"
                     /pseudo
     CDS             82395..83243
                     /locus_tag="OO369_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(83281..84342)
                     /locus_tag="OO369_000087"
     CDS             complement(83281..84342)
                     /locus_tag="OO369_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5980511.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            84555..86018
                     /gene="bepA"
                     /locus_tag="OO369_000088"
     CDS             84555..86018
                     /gene="bepA"
                     /locus_tag="OO369_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR5980512.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            86039..86398
                     /gene="arsC"
                     /locus_tag="OO369_000089"
     CDS             86039..86398
                     /gene="arsC"
                     /locus_tag="OO369_000089"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR5980513.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(86531..87277)
                     /gene="hda"
                     /locus_tag="OO369_000090"
     CDS             complement(86531..87277)
                     /gene="hda"
                     /locus_tag="OO369_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR5980514.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(87327..88616)
                     /gene="uraA"
                     /locus_tag="OO369_000091"
     CDS             complement(87327..88616)
                     /gene="uraA"
                     /locus_tag="OO369_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR5980515.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(88702..89328)
                     /gene="upp"
                     /locus_tag="OO369_000092"
     CDS             complement(88702..89328)
                     /gene="upp"
                     /locus_tag="OO369_000092"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR5980516.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            89652..90689
                     /gene="purM"
                     /locus_tag="OO369_000093"
     CDS             89652..90689
                     /gene="purM"
                     /locus_tag="OO369_000093"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR5980517.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            90689..91327
                     /gene="purN"
                     /locus_tag="OO369_000094"
     CDS             90689..91327
                     /gene="purN"
                     /locus_tag="OO369_000094"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR5980518.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            91499..93565
                     /gene="ppk1"
                     /locus_tag="OO369_000095"
     CDS             91499..93565
                     /gene="ppk1"
                     /locus_tag="OO369_000095"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR5980519.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            93570..95111
                     /gene="ppx"
                     /locus_tag="OO369_000096"
     CDS             93570..95111
                     /gene="ppx"
                     /locus_tag="OO369_000096"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR5980520.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(95150..>96256)
                     /gene="pdeF"
                     /locus_tag="OO369_000097"
     CDS             complement(95150..>96256)
                     /gene="pdeF"
                     /locus_tag="OO369_000097"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5980521.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        23344 a        25102 c        24240 g        23570 t
ORIGIN      
        1 tcacgtaaag cttgtcgagt tgacgggcgg catcatatga attccgcccg aatgtgtcaa
       61 cggcaaattg caacgtgtag tttcaatcgc tgaaaaatca ggcaaatgaa caattttgga
      121 aaaccgctcg cattcagtag ttattcatgt cacggtttcc tgtaaagtgg tgttataaaa
      181 tgaactacta atagacccac atacattcag ggaattgtta tgttcaagga gaggatgaca
      241 ccagatgaac ttgccagact gaccggctat agccgccaga ccattaataa atgggtacgc
      301 aaggaaggct ggacaacgtc accaaaacct ggcgtccagg gtggcaaagc ccgactggtt
      361 cacgtcaatg aacaggttcg tgaatatatt cgcaatgctg aacgtccaga aggtcaggga
      421 gaagcgcctg ccctttctgg tgatgcgccg cttgaagtat tgctggtgac actggcgaaa
      481 gagatgacgc cagttgaaca aaaacagttt acatccttgc ttctgcggga agggattatc
      541 ggattgttac aacgcttagg gattcgcgat agcaaataat atgaaaagat tacgcaataa
      601 aatgaccacc gaagaactgg ctgaatgcct cggtgtggct aaacaaactg ttaaccgttg
      661 gaccagagaa aaaggctgga aaacggaaaa atttcccggc gtgaaaggcg gtcgtgccag
      721 acttattctg gtcgatacgc aagtttgcga gtttattcag aacacgccag ccttccataa
      781 cacaccaatg ttgatggaag ctgaagagag aattgctgaa tacgctcctg gagcacgcgc
      841 cccggcgtat cgacaaatca tcaacgcgat agataatatg acagacattg agcaggaaaa
      901 agtcgcgcaa tttttgtcac gtgaaggaat tcgcaatttc ctcgcccgtc tcgatattga
      961 cgaatcagca taaataaaaa acggcaggat attatctcct gccgtttatc tttttacacg
     1021 ctaattactg ctgattttcg cgttcagcaa taaaatccag cgctttgttg atacgctcga
     1081 tactgcgggt cttaccaatt gcgtgaacgg taacatccag tgctggagac tgccccgcac
     1141 cggttacggc gacacgcagc ggcataccaa ctttacccat acccacttcc agctcatccg
     1201 ccgtcgcctg aatagcgtga tgaacgtttt cagcggtcca gtcagtaatc gcggccagtt
     1261 tgtcacgaac cacttccagc ggctgacgcg ctaccggacg cagatgtttt ttcgccgcat
     1321 cggcatcgaa ctcagcaaaa tcttcgtaga aataacggca gctctgtgcc atctctttca
     1381 gcgtcttgca gcgttcgccc agcagtttca ccagatcagc cagctgcggg ccgttacggg
     1441 tatcgatatt ttcctgctca atgtgccact gtaagtgagt agcaacatac tccggcggca
     1501 gcgcgttaat gtagtgatgg ttcagccaca gcagcttgtc ggtgttgaac gcactggcag
     1561 atttgctgac ggcattcaaa gtgaagtatt tgatcatctc ttcacgagtg aagatttcct
     1621 gatcgccgtg ggaccagccc agacgcacca gatagttcag cagtgcttct ggcaaataac
     1681 cgtcatcacg atactgcatt acgctgactg ccccgtgacg tttggacagt tttttaccgt
     1741 catcgccatt gatcatagaa acgtgcgcgt aaaccggcac cggcgctttc agggccttaa
     1801 gaatgttgat ctggcgcggc gtgttgttga tatggtcttc gccacggata acgtgggtga
     1861 tttccatatc ccagtcatcg acaaccacac agaagttata ggttggggaa ccatcggtac
     1921 ggcggataat aagatcgtcc agttcctggt tgctgaactc gatcggacca cggatctgat
     1981 cgtcaaaaac aacagaacct tcctgcgggt tagcaaaacg tacaacacac ggttcatcat
     2041 cagcatgatg ctcatggctg tggcggcagc gaccgtcata acgcggcttc tcacctttcg
     2101 ccatttgctc ttcgcgcagc gcttccaggc gctctttaga gcaatagcat ttataagcag
     2161 tgccctcttc cagcatctga tcgatcaccg cgttgtagcg atcaaaacgt ttggtctggt
     2221 agtacggacc ttcatcccac tccaggctca gccagttcat gccatccata atggcttcga
     2281 tagcttccgg cgtggaacgc tcaagatcgg tgtcttcaat acgcagcacg aactcaccgc
     2341 cgtggttacg tgcaaaaagc caggagtaaa gagcagtacg cgcgccgcca acgtgcagat
     2401 agcctgttgg gcttggcgcg aagcgagttt tgattttcat gaaatggcct tacgtttaga
     2461 aagatgccga caaccggcaa atcctggaaa aattgagtgg gcgatattct atcactcccg
     2521 cctgattcct caatgtagtt cgggtttcta ccttacgcat tattgttttc gttgacaaat
     2581 tgcgcaacac ggtctgtttt gcgatcatct tgcttaaatt tacgacgaac gaacaatttc
     2641 tttagaaaat gcgttgactc attttgaact ctccctataa tgcgactcca cacagcgggg
     2701 gtgattagct cagctgggag agcacctccc ttacaaggag ggggtcggcg gttcgatccc
     2761 gtcatcaccc accaactact ttatgtagtc tccgccgtgt agcaagaaat tgagaagtgg
     2821 gtgattagct cagctgggag agcacctccc ttacaaggag ggggtcggcg gttcgatccc
     2881 gtcatcaccc accacttcgg gtcgttagct cagttggtag agcagttgac ttttaatcaa
     2941 ttggtcgcag gttcgaatcc tgcacgaccc accaatgtaa aaaagcgccc taaaggcgct
     3001 tttttgctat ctgcgatact caaagattcg aacctgcagc aggtttgagt tgagcgcagc
     3061 gaaacaacgg agccgctcgc ggcgacggcc cgaagggcga gcgaagcgag tcatcctgca
     3121 cgacccacca atgcaaaaaa gcgccctaaa ggcgcttttt tgctatctgc gatttgtaag
     3181 attgattttt cttctcaagt tccttgttct tctaccgata ttacctcgta cagtgacgag
     3241 gactgaacaa tggccactat ctcttcaact tcaattcctt ccatacaaac acagagtagt
     3301 aacagggcat cacaaggtag cgatgttgct tcacaaatcg cgcggataag tcagcaaatc
     3361 atcaaactca ctcaacaaat taaagagatt gtagacacta gcggcagtgc tgaagacaag
     3421 caaaaacagg ccgaattgat tcaacagcaa atcaccctac tggaaacaca actggcgcag
     3481 ttgcagaaac agcaggcaga aaaggcgcag gagaaagagc agcgtttgtc tctgaatgta
     3541 tcccttctaa atcctgttga aaaaacgact catatcgata tctatatctg atacaacgga
     3601 tccccttccc gaccagccat tagcgcattg ccctggtctc gtaccacgtc ccataacgct
     3661 tcagcggcgg tggaaagtga acggttcttc ctgcgcacca gcatcaactg gcgttcaacc
     3721 accggcgtaa tgcgcttaac caccagtggg ctaccttcag gcaatggtag cgccagtgca
     3781 ggaagaatac tgatgccgat gcctgcggct accatcggga acagcgtcgc cggatgtcca
     3841 atctcctgca caatattcgc ctgaatacca ttgcgcgcca gcgctgcgtc aatcagcggt
     3901 cggctgcctg acgcgtaatc ctgcaacacc aatttcgctc cctgtaatgc ctgccagggt
     3961 acataatctt ccacggccaa agcgctatcg cgatggcaaa gcagaaagaa aggctcggaa
     4021 agaatcgctt cacattgcag atcgcccact gggcctggat cgatgacgat gccaaagtcg
     4081 acatctccct ggcgaatact ttccatcacc cactgctgcg gacgatcatg caggacaaac
     4141 tgaatatctg gatagcggcg atggctttca gcaatgcatt gcggaataag atgcgcggaa
     4201 atggtctggc tggcagcgac ccgaactttt ccgctcagtt gttgccccat acgcccggta
     4261 tcgcgcaacg tgctgttaag ttcatccagc agtcgctcaa gacgcaaagc caactgctgc
     4321 cctgcatctg taagcaccac ttcacgcgtg gttctgtcca gcaggcgaac accagtatga
     4381 ttttccagct ccttcacact gtgacttact gccgactggc tcaagccgat acgctctcct
     4441 gcacgactaa aacttttctc ctgcgctact gtgacgaaaa cctttaattg ttttaaagaa
     4501 taattcatct gttttcttca tgaatggatg caataaatca attttatttc tcaaactgga
     4561 agaagcacaa tagaaccatc gatcatctgg agtctttatg aaactttttc gtatcctcga
     4621 tcctttcacc ttaaccctga tcacggtggt gttgctggcc tctttctttc cggccagagg
     4681 cgatttcgtc cccttctttg aaaatctgac caccgcagct attgccctgc tgttctttat
     4741 gcacggcgcg aagttgtcgc gtgaggcgat tattgctggc ggtggtcact ggcgactgca
     4801 tttgtgggta atgtgcagca ccttcgtgct gtttccgatt ctgggtgtac tgtttgcctg
     4861 gtggaaaccg gtaaatgtcg acccgatgct ctactccggt tttctctact tgtgcattct
     4921 cccggctacc gtgcagtctg caatcgcctt cacgtcaatg gcgggcggta acgtcgcggc
     4981 ggcggtttgt tctgcgtcag catccagcct gctggggatt ttcctttcac cattgctggt
     5041 tggtctggtg atgaatgttc acggtgcaag gggcagcctt gagcaggtcg gtaaaattat
     5101 gctgcaactg ctgctgccgt ttgtgttggg gcatctttcc cggccgtgga ttggtgactg
     5161 ggtgtcgcgc aataaaaaat ggattgcgaa aactgaccag acgtccattc tgttggtggt
     5221 ttatacagcg ttcagcgaag ccgtcgttaa tggtatctgg cataaagttg gctggggatc
     5281 attgctgttt atcgtggtgg tcagctgcgt tcttctggct atcgtgattg tagttaacgt
     5341 ctttatggca cgccgactga gcttcaataa ggcagatgaa attactatcg tcttttgtgg
     5401 ttcgaaaaag agtctggcaa atggcatccc gatggcaaac attctgttcc ccacatcggt
     5461 gatcggtatg atggtgctgc ccctgatgat tttccatcag atccaattga tggtctgtgc
     5521 ggtgctggcg cgtcgataca aacgccagac cgaacagtta caggcgcagc aggaaagcag
     5581 cgccgataaa gcttaaagcg gacgcttcag gggctggacc agttgcgtca gcccctcggt
     5641 tttaatcagc agcgtgattt gcattagctc accgagcttc cctgccggga actcatcttt
     5701 gcgggcaaac cacagcagat actcctccgg caggtcaatt aagcgacgcc ctttgtattt
     5761 gccaaacggc attatcgtat tggctatttc aatcagctgc tctttttcca tctcagctac
     5821 ccagcaaacg cagcatttcc gcttcgtcga tgacttcaat gcccagttcc tgcgccttcg
     5881 ccagtttaga tcctgcagct tcacccgcta tcaccagatc ggttttcttc gacacgctgc
     5941 ccgcgacttt cgcgcccagt tcgaccagtc gagctttagc gtcatcacgc gacatctggc
     6001 ttaagctgcc cgtaagcacc acggttttac cagcaaacgg gctgtcaatc tcttccgcgt
     6061 tgataacgat cggcgcaggc cagtgaacac cttccgccaa cagctcgctg atgacattgc
     6121 ggttgctttc ttcggcaaag aagttgtgaa cgtgggatgc aacgacaatg ccaacatcag
     6181 gcaccttttg cagctcttca atcgaagcgg cttccagcgc ttccagcgtg ccgaaatatg
     6241 ccgccagacc tgctgcggtg gcctcgccga cttcacggat gccaagtgca tagaggaagc
     6301 gagcaaaggt ggtttctttc gctttttcca gcgcgttaac cacgttttgt gccaattttg
     6361 gccccatacg ctccagtccg gtcagtttgc ctgcggtgag tttgaacaga tctgccggag
     6421 tatggacata ttctttttca accagctgat cgatgatttt gtcgcccatt ccgtcaacat
     6481 ccatcgcacg gcgggaaaca aagtgtttca gcgactcttt acgctgcgca ccgcaaatca
     6541 ggccaccggt acagcgggca accgcttcac cttccacacg ctcaacgtca gaaccacata
     6601 ccggacaatg cgtcgggaat acaacctcac gggtatcttc cgggcgttca gaaagcacga
     6661 cgttaaccac ctgcgggatc acgtcgccag cgcggcgaat caccacttta tcgccaatgc
     6721 gtaaaccaag acgttcgatt tcatccgcat tgtgtaaggt tgcgttactc accagcacgc
     6781 cagcaacatg gacaggttcc agacgcgcaa caggcgtaat cgcgccagta cgcccaacct
     6841 gaaactcgac gtcacgcaca aaggtcatct gctcctgcgc cgggaattta aacgcaaccg
     6901 cccagcgcgg ggcacgcgcg acaaagccaa gctgctcctg ctgttccagt gagttgacct
     6961 taatcaccac gccgtcgata tcaaagccca gcgtcgggcg gtcttcttcc actttgtgat
     7021 agaacgccag cacttcttcc gccgattcac aaagcgttac ccgatcgctg accggcaccc
     7081 cccacttttt aaattgcagt aaacggccaa gatgagtatc cggcagctcg ccaccttcca
     7141 gaacaccaac gccatagcag aaaaaagtga gcggtcgctt cgctgtaata cgcggatcaa
     7201 gctgacgcag tgaaccagct gccgcattac gtgggttagc aaaaactttc ccgcccgtgc
     7261 gtcgcgcatc ttcgttaatc ttttcgaacc ccgcctgcgg caggaacact tcaccacgca
     7321 cttccagacg cgccgggata ttctctccgt gcagcttcag cggaatggcg cgaatagtac
     7381 gcacattaga cgtgatatct tccccggtgg tgccatcgcc acgggtcgcg gcactgacta
     7441 aaacgccatt ttcatacaga atactgacgg caaggccatc cagcttcagc tcacagcacc
     7501 aggtgacttt ctcgttgttt ttcagacggt cctgcacacg tttgttgaaa gcaagaaagc
     7561 tttcttcatc aaaaacgtta tccagtgaca gcattggtac ttcatggcgt atctggctga
     7621 aagctgccag cggcgcagca cctacacgtt gggtaggcga atcaggcgta atcagttctg
     7681 gatgtttggt ttccagctcg cgcagttcgc gcatcagcct gtcgtattca gcgtcgggaa
     7741 tttccggcgc atccatcaca tgataaagat attcatgatg gcaaagcgtc gttcgcagtt
     7801 ctgtcagttg ttgttcgatt gattccatat cgcaccatca atgctaaaaa cccccgacaa
     7861 gcgggggttc gaagaggagt taatttgcct taagtgtatc aggcgttggc gtctttgact
     7921 tcgcggatga tgtcctggta ctcgcgcaat ttctgcggag tcatcatacg gcgctgatcg
     7981 tcaagcacga caccgcccac ttcatcggca atatgctgcg cagattgcag catcagcttg
     8041 aagttctgca gctcgtcacc gtaagacggt acctgcataa agatagtgac acccggagta
     8101 gtgaaatcct tcatttcagg atcaaaggtt cccggtttca ccatattcgc caggctgaat
     8161 aacgccgggc cgctgccatc cgggctaaga tgacgatggt aaatattcat atcgccaaaa
     8221 atgaagcccg cttgttgaat gctgttaaga agcagttcac cgtttagctc gctaccgtga
     8281 tgcgccgcga cgttcatgat aatcaccgct tctttgcgct tcggtttatc cataactgga
     8341 gccggttccg ctacaggctc aggctgtggt gccgctacgg gttctgcagg ctggaaagcc
     8401 tgttgtgccg gttgcggtgc tgaatgcaca ggctgcggcg ctggcgcgac ctgtggcgaa
     8461 actggctgct gcaacggctg ttcaggctgc ggctgatagg caggctgctg caccggctgc
     8521 gctggacgcg gagcatgttg cggcggtacc tgcgcttcag gcggctgctg gaccggttga
     8581 cgcggctgcg cagacgcata aggcggtagg tactggtgtt gcggcgacgg acgagcagcc
     8641 tcatgctcct gagcgttagc cggggcatga ttcacgcggt gaacacgaac ctcaccaacg
     8701 ccctcatcat cttcgacatc ctcgtcataa gaatcgtcgt cacgttttga cttcattcgt
     8761 tttaatggcc gatcgcggaa catagaagat cgttctttac ggctggtcca gaaaccatgt
     8821 accagtaaag cgattatggc gatcgcgcca acaatgatta atatcagacg caaatcctgc
     8881 atcattatat tctctgttgt tctaacacct tgccaccacg gcaaacattt actcactaag
     8941 agtatttgcc gattacctca agtgcaagtg cactattaac tttcacagca caaagataga
     9001 tgaaatcgtg ctttttgctg ttttttcgaa catatcctaa ctgtccattg cgcaattacc
     9061 cggtaaaata cgcagaattt tcctgggatt ggtcaaaagg agctcatcct gattatggtt
     9121 tcatcattca catctgcccc acgcagcggt ttttactatt ttgcgcaagg ctggaagctc
     9181 gtctcgcaac ctgggattcg gcgtttcgtt attttaccgc tgctggtcaa tattttgttg
     9241 atggggggcg cattctggtg gctctttaca cagctcgatg tctggatccc gactttcatg
     9301 agttacgttc cggactggct acaatggctg agttatctgt tgtggcctct ggctgtcatc
     9361 tctgtgctgt tagtgtttgg ctatttcttc tccacgattg ctaactggat tgccgctccg
     9421 tttaacggtt tattggctga acaactggaa gcacgattga ctggcgctac accgccagat
     9481 accgggattt tcggtatcat gaaagatgtg ccgcgaatca tgaaacgcga atggcaaaaa
     9541 tttgtctggt atctgccgcg cgcaattgta ttactaattc tttacttcat ccctggtatt
     9601 gggcaaaccg tcgcgccggt actgtggttc ctgtttagcg cctggatgtt agccatccag
     9661 tattgcgatt accccttcga taaccacaaa gtgccgttta aagagatgcg caccgccctg
     9721 cgcacacgca aaatcaccaa tatgcagttt ggtgctttaa ccagcctgtt tacgatgatc
     9781 ccgctgctta atctgttcat catgcccgtt gccgtttgtg gcgcgacggc gatgtgggtc
     9841 gattgctatc gcgataaaca cgcgatgtgg cggtaacaat ctaccggtta ttttgtaaac
     9901 cgtttgtgtg aaacaggggt ggcttatgcc gccccttatt ccatcttgca tgtcattatt
     9961 tcccttctgt atatagatat gctaaatcct tacttccgca tattctctga gcgggtatgc
    10021 tacctgttgt atcccaattt catacagtta aggacaggcc atgagtaaga tttttgaaga
    10081 taactcgctg actatcggtc acacgccgct ggttcgcctg aatcgcatcg gtaacggacg
    10141 cattctggcg aaggtggaat ctcgtaaccc cagcttcagc gttaagtgcc gtatcggtgc
    10201 caacatgatt tgggatgccg aaaagcgcgg cgtgctgaaa ccaggcgttg aactggttga
    10261 accgaccagc ggtaataccg ggattgcact ggcctatgta gctgccgctc gcggttacaa
    10321 actcaccctg accatgccag aaaccatgag tattgaacgc cgcaagctgc tgaaagcgtt
    10381 aggtgcaaac ctggtgctga cggaaggtgc taaaggcatg aaaggcgcaa tccaaaaagc
    10441 agaagaaatt gtcgccagca atccagagaa atacctgctg ctgcaacaat tcagcaatcc
    10501 ggcaaaccct gaaattcacg aaaaaaccac cggtccggag atctgggaag ataccgacgg
    10561 tcaggttgat gtatttattg ctggcgttgg gactggcggt acgctgactg gcgtcagccg
    10621 ctacattaaa ggcaccaaag gcaagaccga tcttatctct gtcgccgttg agccaaccga
    10681 ttctccagtt atcgcccagg tgctggcagg tgaagagatt aaacctggcc cgcataaaat
    10741 tcagggtatt ggcgctggtt ttatcccggc taacctcgat ctcaagctgg tcgataaagt
    10801 cattggcatc accaatgaag aagccatttc taccgcccgt cgtctaatgg aagaagaagg
    10861 tattcttgca ggtatctctt ctggagcagc tgttgccgca gcgttgaaac tacaagaaga
    10921 tgaaagcttt accaacaaga atattgtggt tattctacca tcatcgggtg agcgttattt
    10981 aagcaccgca ttgtttgccg atctcttcac tgagaaagaa ttgcaacagt aatgccagct
    11041 tgttaaaaat gcgtaaaaaa gcaccttttt aggtgctttt ttgtggcctg cttcaaactt
    11101 tcgcccctcc tggcattgat tcagcctgtc ggaactggta tttaaccaga ctaattattt
    11161 tgatgcgcga aattaatcgt tacaggaaaa gccaaagctg aatcgatttt atgatttggt
    11221 tcaattcttc ctttagcggc ataatgttta atgacgtacg aaacgtcagc ggtcaacacc
    11281 cgccagcaat ggactgtatt gcgctcttcg tgcgtcgcgt ctgttaaaaa ctggcgctaa
    11341 caatacaggc taaagtcgaa ccgccaggct agactttagt tccacaacac taaacctata
    11401 agttggggaa atacaatgtt ccagcaagaa gttaccatta ccgctccgaa cggtctgcac
    11461 acccgccctg ctgcccagtt tgtaaaagaa gctaagggct tcacttctga aattactgtg
    11521 acttccaacg gcaaaagcgc cagcgcgaaa agcctgttta aactgcagac tctgggcctg
    11581 actcaaggta ccgttgtgac tatctccgca gaaggcgaag acgagcagaa agcggttgaa
    11641 catctggtta aactgatggc ggaactcgag taatttcccg ggttctttta aaaatcagtc
    11701 acaagtaagg tagggttatg atttcaggca ttttagcatc cccgggtatc gctttcggta
    11761 aagctctgct tctgaaagaa gacgaaattg tcattgaccg gaaaaaaatt tctgccgacc
    11821 aggttgatca ggaagttgaa cgttttctga gcggtcgtgc caaggcatca gcccagctgg
    11881 aaacgatcaa aacgaaagct ggtgaaacgt tcggtgaaga aaaagaagcc atctttgaag
    11941 ggcatattat gctgctcgaa gatgaggagc tggagcagga aatcatagcc ctgattaaag
    12001 ataaacacat gacagctgac gcagctgctc atgaagttat cgaaggtcag gcttctgccc
    12061 tggaagagct ggatgatgaa tacctgaaag aacgtgcggc tgacgtacgt gatatcggta
    12121 agcgcctgct gcgcaacatc ctgggcctga agattatcga cctgagcgcc attcaggatg
    12181 aagtcattct ggttgccgct gacctgacgc cgtccgaaac cgcacagctg aacctgaaga
    12241 aggtgctggg tttcatcacc gacgcgggtg gccgtacttc ccacacctct atcatggcgc
    12301 gttctctgga actgcctgct atcgtgggta ccggtagcgt cacctctcag gtgaaaaatg
    12361 acgactatct gattctggat gccgtaaata atcaggttta cgtcaatcca accaacgaag
    12421 ttattgataa aatgcgcgct gttcaggagc aagtggcttc tgaaaaagca gagcttgcta
    12481 aactgaaaga tctgccagct attacgctgg acggtcacca ggtagaagta tgcgctaaca
    12541 ttggtacggt tcgtgacgtt gaaggtgcag agcgtaacgg cgctgaaggc gttggtctgt
    12601 atcgtactga gttcctgttc atggaccgcg acgcgctgcc cactgaagaa gaacagtttg
    12661 ctgcttacaa agcagtggct gaagcgtgtg gctcgcaggc ggttatcgtt cgtaccatgg
    12721 acatcggcgg cgacaaagag ctgccataca tgaacttccc gaaagaagag aacccgttcc
    12781 tcggctggcg cgctatccgt atcgcgatgg atcgtaaaga gatcctgcgc gatcagctcc
    12841 gcgctatcct gcgtgcctcg gctttcggta aattgcgcat tatgttcccg atgatcatct
    12901 ctgttgaaga agtgcgtgca ctgcgcaaag agatcgaaat ctacaaacag gaactgcgcg
    12961 acgaaggtaa agcgtttgac gagtcaattg aaatcggcgt aatggtggaa acaccggctg
    13021 ccgcaacaat tgcacgtcat ttagccaaag aagttgattt ctttagtatc ggcaccaatg
    13081 atttaacgca gtacactctg gcagttgacc gtggtaatga tatgatttca cacctttacc
    13141 agccaatgtc accgtccgtg ctgaacttga tcaagcaagt tattgatgct tctcatgctg
    13201 aaggcaaatg gactggcatg tgtggtgagc ttgctggcga tgaacgtgct acacttctgt
    13261 tgctggggat gggtctggac gaattctcta tgagcgccat ttctatcccg cgcattaaga
    13321 agattatccg taacacgaac ttcgaagatg cgaaggtgtt agcagagcag gctcttgctc
    13381 aaccgacaac ggacgagtta atgacgctgg ttaacaagtt cattgaagaa aaaacaatct
    13441 gctaatccac gagatgcggc ccaatttact gcttaggaga agatcatggg tttgttcgat
    13501 aaactgaaat ctctggtttc cgacgacaaa aaggataccg gaactattga gatcattgct
    13561 ccgctctctg gcgagatcgt caatatcgaa gacgtgccgg atgtcgtttt tgcggaaaaa
    13621 atcgttggtg atggtattgc tatcaaacca acgggtaaca aaatggtcgc gcctgtagac
    13681 ggtaccattg gtaaaatctt tgaaaccaat cacgcgttct ctatcgaatc tgatagcggc
    13741 gttgaactgt tcgtccactt cggtatcgac accgttgaac tgaaaggcga aggcttcaag
    13801 cgtattgctg aagaaggtca gcgcgtgaaa gttggcgata ctgtcattga atttgatctg
    13861 ccgctgctgg aagagaaagc caagtctacc ctgactccgg ttgttatctc caacatggac
    13921 gaaatcaaag aactgatcaa actgtccggt agcgtaaccg tgggtgaaac cccggttatc
    13981 cgcatcaaga agtaattctt gccgcagtga aaaatggcgc ccatcggcgc cattttttat
    14041 gcttccgcca gcggcggcaa aatcaattca tcgctctcat gctgctgggt gtagcgcatc
    14101 acttccagta cgcgcaaccc cgcacggtgc accgcatcgt ttaacgcctt ccctttcagc
    14161 aaaccactga taagctgagc acaaaacagg tcgccagtcc ctttcaggtc ggtttttacc
    14221 cgtgaatggg aaatgacatt cacgctgtcg gcactgacca ccaccacctg catctcctga
    14281 ttttcttcat taccggaggc gctggtaatc accacccatt ttaatgtgtc tgaaagcaga
    14341 ctttttgcgg cagcaatggc actgtcgaga tcgcggcaat ttttaccggt caggatttcc
    14401 agctcaaaga tattgggggt aattccctgc gccagcggca gtaaatattg tcgatacgcc
    14461 tcgggaaggt caggtttgac ataaattccg ctatcaatat cgccaatcac cggatcgacc
    14521 atgatcaata ggtcaggatg gtctttgcgt agcgcagtca gccactcggc aaggattttg
    14581 atttgcgatg ccgttcccat atagcccgtg gttacagcac gaagttggcg cagcgcatca
    14641 cgctcctgaa gcgcacgcaa atagccgcta aaccattcgt ccggaatcgc accaccgtag
    14701 aaagtgtcat aatgcggcgt attgctcagc aataccgtcg gcacggcaaa gacattcaag
    14761 ccgttctgtt ttatagcagg caccgcaatg ctgttgccta cgcttccgta cactacctgc
    14821 gactgcacgg cgacgatatc cgcctgcagc gccctactct tatcgttaaa caacaacaaa
    14881 ctactcattt aattttttct ccttgccgat gatcctcatc gtattccaac cgaaacttta
    14941 cctgattctg gcagtcaaat cggctattac aaaacaaaga taaggtaatt cagtgaagaa
    15001 aatcatttgt ctggccatta cgctgctaat gacactcccc gcttacgcga agttaactgt
    15061 tcatgaagaa gcccgcatca acgccatgct ggagggatta gcacagaaaa aggatttgat
    15121 atttgtgcgc aacggtgatg aacatacctg cgatgaagcg gtttcccacc tgcgtctgaa
    15181 gctcggcaat acccgtaacc gcattgacac tgccgagcag tttattgata aggtagcttc
    15241 gtcgtcatcg attaccggga agccgtatat cgtgaagatc cccggtaaga gcgatgagaa
    15301 cgcacagcct tttttacatg cgttaattgc gcagacggat aaaacggtgc ctgcggaagg
    15361 aaattaatcc gctttggtaa ggcatttaca ggaggtaaca tgaaaaaatg ctttatttat
    15421 cacgatgaaa aatcgaataa attttggtgg atagattacg aaggggatag tttagctgtc
    15481 aactatggca aggtaggtag tattggtaaa ttccatacaa aagaattcga taatgaagaa
    15541 cagtgtctga aagaagccag taaattgatt gccgcaaaaa tgaaaaaagg atatcaagaa
    15601 actccagact ttaacttcat ggatctctac tattttgatg atgaagagat tgggttacat
    15661 cttaaaacgt cacacccaaa cttccagtgc cattttactg atccacttta tatgtgttgc
    15721 tgggatgaag aatctccttt tggtagcgat gaaggtgctg atgctctaaa cgaacttgaa
    15781 aatagcctcc gttctggact gtgctgattt ccctcaaatg ttaattgaaa ctatgtgggg
    15841 tatgaaatac atcgctatgg acagtattct tgaagaggat gtgcgtgcgc aattactagc
    15901 cgatgaaatg agctctatcc agagcaatat gattacctac gcgactgcat tcggtcagat
    15961 caaagtcatg ggtaaaatct cccataaact taaaaagatg ggactcaatg cactagcgcg
    16021 tcatcagctt accgcaaaaa ttcttcaatg gggtgacggt caggactcac caatacttca
    16081 aaaaatgatt gatgacctta ctgcttttcc acacgaaaat taaaaactgc atttgtcggc
    16141 agcaacaact gccgacaaat cagattattg acaaagtgca ctttgtttat gccggatgcg
    16201 gcgtaaacgc ctgatccggc ataaaaatat tgctaattca atatattgca gaaacattgt
    16261 aggcctgata agcggagcgc atcaggcagt tttgcgtttg tcatcagtct ccaatgctat
    16321 taatccttaa atccctgccc cctgactaaa atgctcttcc ccaaacactc cggtggagag
    16381 gtagcgatcg ccacgatcgc agatgatcgc taccaccacc gcaccagggt tagcttttgc
    16441 cacccgcagt gctccagcaa ccgcgccgcc tgagctgacg ccacagaata ttccttcccg
    16501 caccgccagt tcgcgcatgg tgttttccgc atcgcgctga tgaatatcca gcacctcatc
    16561 caccagcgca gcgttgaaaa tccccggcag atattccgca ggccagcggc gaatgcccgg
    16621 aatactgctg ccttcttccg gttgcaggcc aacaatggtc accggtttgg attgttcgcg
    16681 cataaagcat gaaacgccgg tgatagtgcc ggtcgtcccc atgctggaga caaaatgagt
    16741 gatgcgcccg ccggtttgct gccagatttc cggcccggtg gtggtgtaat gcgcataagg
    16801 gttatcggga ttattgaact gatcgagcag ctttccttcg ccacgattcg ccatctccag
    16861 cgccagatcg cgcgcacctt ccatgccctg ctctttggtg acaagaatca gttccgcacc
    16921 ataagcacgc atcgccgcac ggcgttcctg gctcatgttg tcgggcatca gcaatttcat
    16981 gcgatagcct ttcagcgcgg caatcattgc cagcgcaatg ccggtgttac cactggtggc
    17041 ttcgattaag acatcacccg gtttaatttc cccgcgcttt tccgcctcga cgatcatcga
    17101 aagtgccgca cgatctttca ccgaacctgc cgggttattg ccttccagtt ttaaccacac
    17161 ttcactgccg ttatccggcc ccattcgctg caacttcacc agaggcgtat tgcctattgt
    17221 ttgttctaat gtactcacaa tctctatcca tacgtggtgt tgcctgatgc gacgcttgcg
    17281 cgtcttatca ggcctacagg ttacaaacct tgccattaaa aagcccggat cgcgggaagc
    17341 gcctccgggc gtttaacatt cactcaacct atcaggcgct ttgtgcgaga gcaagttcct
    17401 catcgcgggt ttcaatacgc tcgtcgccgt tatacagccg cgcatgttgc agaccaacga
    17461 ataaacgctc gccatgctgc ggggcatcgt cgccatgcat cacgaccgtc agcggttcgt
    17521 tgtaccaccc cagcggctgc accactaatt gggtgtagtg acctttcggg ctggcttcca
    17581 gtacctgtac cggcagcggc gaatcgagac tggtacggcg gctgatatcc acttcccacg
    17641 ggcgcaggaa gagatccacc ggcccctgat acgcaggtgt gtagcccagc ggccagcgat
    17701 gcgcgccaac atggaactgc ccgccgcgaa tggttccctg caggcggttc acttcgccca
    17761 taaattcgag tacaaaacgg gtcgctggtt cgcgccatac ctgattcggc gcatcagcct
    17821 gttcaatatt cccctgactc atcaccacta cacgatcggc tacttcggtc gcctcttcct
    17881 ggtcgtgggt cacaaacacg ctggtgaatt tcagctcttc atgcagttga cgcagccagc
    17941 gacgcagctc tttacgcacc tgcgcatcca gcgcgccgaa cggttcatca agcagcagaa
    18001 tttgcggttc tacagccagc gcgcgcgcca gcgccacacg ctgtttctgg ccgccggaaa
    18061 gctgcgccgg ataacgatcc gccagatgcg caagctggac catttccagc aattttgtca
    18121 ctttcgcttt gatggctgcg gcattcgggc gctcgcgacg cggcagcacc gtcaggccaa
    18181 aagtgatatt gtcgaatacc gtcatatggc ggaacagcgc gtaatgctgg aacacgaaac
    18241 cgactttacg atcgcgcgcg tgcaggcgac tcacgtcggt gccgtggaag cgaatatgcc
    18301 cgctggtttg atgctccagc ccggcgataa tccgcagcag cgtggttttc ccggaaccgg
    18361 acggccccag caacgcgacc atctgaccag aaggaatatc cagtgagata tcgttcagca
    18421 cctgggtgcg accaaaagac ttcttgatat tggcaatctc aatgctcatg atgctcctcc
    18481 tgctgcgcgc gtttttcctg attctccagg cgccactgca acatactctt taaaaacagg
    18541 gtgataatcg ccatcaacgt caacagcgcg gcggcggtaa aggagccgac ggtgttgtag
    18601 tcctgctcca acaattcaat ctgtaacggc agcgaaagtg tttcgccgcg aatcgagccg
    18661 gaaaccaccg acactgcacc aaactcgcca atcgcgcggg cgttggtcag caccacgccg
    18721 taaagcagcg cccagcgaat attcggcaat gtgacgcgac ggaacatctg ccagccggac
    18781 gcgccaagca aaatcgccgc ttcgtcttcc tggctgccct ggcttaacat caccggcacc
    18841 agctcgcgca ccacaaacgg acacgtcaca aagatggtga ccagcaccat tccgggccag
    18901 gagaacataa tttgcagatt atgttcatcc agccagccgc cgagcgggcc gttggagccg
    18961 tagaacagca gatacaccag accggcaacc accggcgata cagcaaacgg aatgtccagt
    19021 agcgtcagca gtaactggcg tcccgggaag ttaaagcgcg tcaccagcca ggccagcaga
    19081 atgccgaaca ccaggtttac cggtacggca atcagcgcga tcatcaccgt cagccagatg
    19141 gcgtgcagca tgtccggatc ggccagattc tgtaaaaccg gcatcagccc cttgctgaat
    19201 gcctgcacga agatgtaaat catcggcacc agcaggatga acgccgaaac cagcatcccg
    19261 atgccaatca gaaaccattt gccccagtta atcgggcgcg cgtcataacg cttcaattgg
    19321 gtaacttccg ccattaatga cctaccacac gccgaccaaa gcgactttgc agagtgttaa
    19381 ttgagaacag cagcagcaga gatgccgcga ggatcaccga agcaatcgcg ctcgctgccg
    19441 ggtaatcaaa ctcctgtaag cgcacaaaaa tcatcagcga cgtcacttcc gtcttccacg
    19501 cgatatttcc ggcgataaaa atcaccgcgc caaattcacc aagactacgg gtaaacgaca
    19561 gcgccacgcc cgccaccagc gccggagaaa gctccggcag caccactttg cagaaactct
    19621 gccagcgcgt tgcaccaagc gtttccgccg cttcttcata ttccgggcct aactcttcca
    19681 gcaccggctg cacggtacgc accacaaacg gaatgctggt aaaggccatc gccaccgcaa
    19741 taccaagcca ggtataggtg actttgatat caaacttcgc cagccattca ccgtaaaaac
    19801 cgtttacgga aaagagcgag gccagcgtta aaccggcgac tgccgttggc agcgcaaagg
    19861 gtaaatccat cagcgcatca agcagcgtgc ggcccggaaa gcgataacgt gttaaaatcc
    19921 acgccatcag cagaccgaaa acgccgttaa aaatcgatgc cacaaacgcc gacagcagcg
    19981 tcactttgta ggccgcgacc acctgcgggt tggtgatcac ctcccagtac tgcgcccagc
    20041 tcatctcaga aagctgcatc accagcgcag agagcggcag cagcaatatc aggcacacaa
    20101 acagcagact ggtgccaagg cttaaagtaa agcccggcag cacgcgtctt gaagaaactg
    20161 caaacattac ttacgccccg ccgctaacag cttgtctaac tcgccgccgc tggtgaagtg
    20221 ggttttcatc acttccggcc atgagccaaa tttgtcttcc acgcggaaca gttcggtctg
    20281 cgggaattta tctttcagtt tgtccatgac ttccgggtta ttcacacggt agtaatagtc
    20341 ggtgatgatg gtctgcgcct gtgggctgta gagccagttc aggtaggctt ttgcggcttt
    20401 ttccgtaccg ttggcctgca catttttatc gacccacgcc accgggaatt ccgccagaat
    20461 attggttttc gggatcacca cttcaaagcc ctgtgcttca tactgtttac ggatgttgtt
    20521 cacttccgat tcaaagctaa tcagcacatc gcccaagcca cgctcggcaa aagtggtggt
    20581 tgcgccacga ccgccggtat cgaacacttc aacgtttttc aggaactggg tcataaactg
    20641 ttcggttttg gctttatcgc caccgtcagc tttatccgct gcgccccatg ccgccagata
    20701 ggtataacgc gcgttacccg acgttttcgg gttcgggaaa atcagcttca cgtcggagcg
    20761 caccaggtcg ttccagtcgt ggatattctt cgggttgccc ttacgcacca ggaagcccat
    20821 ggtggagtag aacggcgagc tattattcgg caggcgcgac tgccagtcag ccgggatcag
    20881 cttgcctttg tcgtgcagga tttgtacgtc ggtcacctgg ttataagtga caacgtctgc
    20941 ttttaagccc tgcaaaatcg ccagcgcctg ttttgatgac ccggcatgag attgttttat
    21001 cgtcagtttg tcgccgccgt tatcttttgc ccattgctgt tcaaacggcg gattcagggc
    21061 ggcaaacagc tcgcgggaga cgtcataaga actgttcagc agttccgttg cctgtacatg
    21121 gcccgccagc agcagagaag cgaccagcgc gagtgagttc tttttcagta agttaacggc
    21181 cattgcgcac ccttataaat ttaatgactt tctaatagcc atcatattta taacggacgt
    21241 gaaaggagta acggttttat ataccgtttg gtgatttgga agttaaaaag ggaataagaa
    21301 cttggttcaa caggcgtaat tgttgcaggc agtttggaca cggacagcgc ggagaaaccg
    21361 gagcgtacac atagtacgtg aggatttcga gcactgcccg gggccaaaat gacaaatgaa
    21421 atagcctggt gaacttagtt caagacgaaa tcctccccac acatgcaggg aggaaacaga
    21481 ggtgaatcag ataccgacgc taaccgtctc cggcagtgtg ctgccgccat caatcacatt
    21541 ctgtgtaccg gttaaatagc tggattcatc cgatgcgagg aaggccgcca gttcgccgac
    21601 ttccagcgga tcggcgaggc gacacagcgg gattgctttt gccatttcag tcagcaccga
    21661 ttctggatct tccgggttcg actggcgggc aatgctttcc gccattggcg tgcggacgta
    21721 tcccgggcag atggcgttaa cgcgaatacc ggattgcgcg tactccaccg ccagcgattt
    21781 agtcaggcca acaatcgccg ctttcgttaa ggcatacgcc gtttcgccag gatcggccac
    21841 catatcacca gtgactgaag acatcatcac aatgcgacca tctttgcgcg caatcatctc
    21901 cggcagcacc gccttcgtga cgttccatac gcctttaata ttgatatcaa tatggaaatc
    21961 gcgatcttca tcgctcatat cgaggaaact gcccagacga caaacgcctg cgttattcac
    22021 caggatatca atgcgccctt ctttttcctt cgcgcgtttg atagctgcgg ctaccgacgc
    22081 cgggtcacgc acatcggcga caaccgccgt acagcgatga ccacgaccac acagttcgtc
    22141 cgctagcttt tcgatctcag gggagatatc cagcaagatt aggttcgcgc catgacgtgc
    22201 aaaagttctg gcaattcctt cgccaattcc ctgcaatgcg cccgtaatca gtgctgtctt
    22261 gcccgtgagt ttacccattt taatgctctc cttgttaata cagagcgtta cactccattc
    22321 cctctgaaaa tacaggataa acaaaatatt gctgcggatc agctcactaa ttcaggaaaa
    22381 tctcaatcca gtcgctgctt tatgcatcat ttcaggcgtt gtgttagctc actgctgcgc
    22441 tgaatcattt tcaatgacgc cacatcattg atgttgcaca agaccaacaa acagtaaatc
    22501 ggtaacagaa ttttgcgccg tgcgggtaga catcgacgag ctacgccatt cggtttcacc
    22561 ggaaacggta tcgagcgtga aatgcgccag ccgccgcagc ggagagtcag ccagagaagt
    22621 aatggcaatc accgttgccc cctgctttcg tgccgcttct gcgcacaaga caatctcttt
    22681 tttactgccg ctgtaagaga tagcgatttg tacatcgcct tttttcaacg cctgggaaac
    22741 ggtggcttgt acgtgcgtgt cggcttcgca ggccacgcga tagccaattt tcatcagttt
    22801 gaacgacaga tcgcgcccca ccagcgccga tccgcccagg cccgttatct ggataaacgg
    22861 tgctttactg atgacctcaa tgattttttg cagccgcgcg tagtcgaaca acgcacaggt
    22921 ttgctccagc gccagctctt tttcgcgatt cagtttgcgg gcaatcactt ccagcgaatc
    22981 atcactggta atcgaactgt gcaggtgcag cgccgtggca ttcgttttct cccggctggc
    23041 actgtattcg ccaattaacg ccatacgcaa ttcagtaaat ccttgcgcgc cgagcttctg
    23101 ggcaaatttc acaatgctcg actggctgat accgagctgt ttcgccatct gccgtgaaga
    23161 gacggatttc agctcgctca ctctggcccg cagaaagtca gcaatttttt gctcgttccc
    23221 ggtaaattca ctttccgcat tgcgtatttt cgtcaggtac aacatcttcc actctcctgg
    23281 gctggcagca aaaattccgc ttaagttctt atgacgctct tcacactctg cgagtgtaga
    23341 ctcaatgatt cctttaagtc tgtcaattcg ggatatatga ttccatacat ctgcaattat
    23401 ttgatcgtaa atagtaaggt caccaccgat gcaactagaa aagatgatta ctgaaggctc
    23461 gaacaccgcc tcggctgaaa ttgaccgcgt atcgacgctg gaaatgtgcc ggattatcaa
    23521 cgatgaagat aaaaccgtac cgcctgccgt tgatcgcgtc ctgccggata tcgccgcggc
    23581 gatcgatgtt atccacgccc aggtcagcgg cggcggtcgt ctgatttatc tcggtgcggg
    23641 aacatccggt cgtctgggga ttctggatgc cagcgaatgt ccgcccacct acggcgtgaa
    23701 accgggtctg gtggttggtt tgattgctgg aggcgaatat gccattcagc acgcggtgga
    23761 aggagctgaa gatagccggg aaggtggtgt taacgatctg aaaaatatta atttaacggc
    23821 acaggatgtg gtggttggca ttgctgccag cggtcgcacg ccgtatgtga ttgccggact
    23881 ggaatacgca cgccagctcg gctgccgcac agtgggaatt tcctgtaatc cggggagcgc
    23941 cgtttcaacc accgctgagt ttgccattac accgattgta ggtgccgaag ttgttaccgg
    24001 ttcttcgcgg atgaaagcag gtacagcgca gaaactggtg ctcaatatgc tttccaccgg
    24061 gctgatgatt aaatccggca aagtgttcgg caacctgatg gtcgatgtgg tcgccaccaa
    24121 cgaaaaactg catgtgcgcc aggtcaatat cgttaaaaac gccaccggat gtaatgccga
    24181 acaagcggaa gcggcgttaa ttgcctgcga gcgcaactgt aaaacggcca ttgtgatggt
    24241 gctgaaaaat ctcgatgccg cagaagctaa aaaacgcctg gatcaacacg gcggctttat
    24301 tcgtcaggtt ttagacaagg aataacccat ggccaaagag atcagcagtg aacttctgaa
    24361 caccattctt acccgtgtcg gcggaccggg aaatatcgcc agttgtggta actgtatgac
    24421 gcgcctgcgt ctgggtgtac atgacagttc actggttgat cccaatatca aaacgctgga
    24481 aggcgtgaag ggcgtcattt tgaccagcga ccaggtgcag gtcgtttttg ggcctggtaa
    24541 agcgcatcgt gccgcaaaag cgatgagcga gctgctgggc gatgcaccgg tacaggatgc
    24601 cgcagaaatc gccgcccaga acaaacgtca gttaaaagcc aaacaaacct ccggcgtgca
    24661 acaatttctc gccaaattcg ccaccatctt cacgccgctg atccccggtt ttattgccgc
    24721 cggtctgctg ctggggatag cgacgttaat tgccacggtg atgcacgttc cggcagatgc
    24781 tcagggcacg ctacccgacg cgctgaattt tatgaaggtg ttcagcaaag gtttgttcac
    24841 cttcctagtg attctggtgg gctataacgc cgcccaggca tttggcggca cgggcgtcaa
    24901 tggcgcaatt attgccgcac tctttttgct cggttataac cccgctgcaa ccaccggtta
    24961 ctacgccggt tttcacgatt tctttggtct gcccatcgat ccgcgcggca atattatcgg
    25021 cgtgctgatt gccgcgtggg cctgcgcccg cattgaaggc atggtgcgcc gctttatgcc
    25081 ggacgatctc gacatgctgc tgacctcgtt aatcaccctg ctgatcaccg ctacgcttgc
    25141 gtacctgatc attatgccgc tgggtggctg gttattcgaa ggtatgtcat ggctgtttat
    25201 gcacctgaac agtaatccgt tcggttgtgc ggttttagcc gggctgttcc tgatcgccgt
    25261 ggtatttggc gtgcatcagg gctttattcc tgtttacctc gcgttaatgg acagccaggg
    25321 attcaacagc ttatttccca tcctttcaat ggcaggcgcg ggccaggtgg gcgcggcgct
    25381 ggcactctac tggcgggcgc aaccgcacag tgcgctacgc agtcaggtac gcggggcgat
    25441 tattcccggc ctgctgggcg ttggtgaacc gctgatttac ggtgtcaccc tgccccgcat
    25501 gaagccgttt gttaccgcct gtttaggcgg cgcggcgggc ggtttgttta tcggattgat
    25561 agcctggtgg ggtctgccga tgggcttaaa cagcgccttt gggccgtctg gtctggtggc
    25621 gctgccgctg atgacttccg cacaaggcat ccttccggca atggcggttt atgctggcgg
    25681 gattctggtg gcatgggttt gcgggtttat tttcaccacg ctctttggct gccgtaacgt
    25741 caatctggac tgatattatg aaacggacaa tgctctatct ttctctgctg gctgttagtt
    25801 gtagcgtcag cgccgcaaaa taccctgttc tgacagaagg tagttgatca agtatatgcg
    25861 gcgttaaagc agtaataaaa aggccagtca gtaatgttac tgcctggcct ttaatcaatg
    25921 aatcagaaac gcttacagcg ccatcaactt gtccagcgac ggtgcgaaat aatagccgcc
    25981 ggttaccggt ttggtgaaac gcaacatcgc atcacgtttg ccatcggtat cgccaaacat
    26041 gctcagcagt tgctgctcaa tgttatgcag acgcgcgcag taggcgcaga agtacagacc
    26101 gtgagtgcca ctggcggtgc cgtacggcag gctctggcga acaatcttca gccctttgcc
    26161 atcttctttc agatcaacgc gggtgaggtg agaggtttcc ggacgttcgt cgccgtcaat
    26221 ctcttcgttg gcctctttgg tgcgcccgat catcatctcc tgatcgtgaa cgctcatccg
    26281 gttgagctgc ttcaggttgt gctcccaacg ctgcacaaac acatagctgc cgccggaatc
    26341 cacgccgtct ttgataaccg ccacttcgcg gcgcgtctct tcacccgccg ggttttctgt
    26401 accgtcaaca aagccgctca ggtcacgctc ttccacccaa cggaagccgt ggatctcttc
    26461 tttcacttca atgcagtcac caaaggcttc catcgccgcc tgagcgacag agaagtttac
    26521 gtcgtgacgc agagaaagaa tgtggatcaa cacatcgaac tgggtcgtcg gcgcaaggcc
    26581 tttaccgtag cccggaaaat ctttcagctc ttctgcccca acgccgccgc tcagagcgcg
    26641 ccaggtgttg ttaccaaagg caaccaccgc accaagatgc gcgtccggga atttcgcttc
    26701 aaaagttgcc agtttgtcgg caaatgtttt actggccgca cgcagggcgt caacttcccc
    26761 tttcacgttg gcttcgatcc aaatcgccgc gcggcaatgt tctggcaaaa tgccactctg
    26821 aacctgagac attgttcctc ctgaaaataa gaatgccacg caatccgtgg cattgttgag
    26881 cgctatttta cgtgtttttt cgcgacggcg gtttgttcag acgcaaatta acgccgccag
    26941 ataattttac tgactttcca gtttttcagg gtatcgtccg aaggcattaa cccttcagga
    27001 ccacgccatt ccccgctgaa ctggtagcta atatgttgac tgccttcggc tttacattcg
    27061 acggcacgat tatcatcacc atcagctttt tggcaattgc caaatgcttt gctgtaaagg
    27121 tcgctaaacg gtgtaccgat tttaacaccg gtgtcagcag gaatatcgct atccagcacg
    27181 tcaatgcgac tgatcgtgcc ttgatcgccg ttaatcacca tcgccacgtt gtcgcctttc
    27241 atcacttcaa aaaagcgcac cacgttgccg ttcgtggttt tcattccgct gcgcaggcga
    27301 taatcgccat caagcgcatc agcaatggct tgttcttgca gtggtgtgga cgccgttaat
    27361 tcacccacgc cctgctcgct cactttggtg gacgatccaa accagttcca cgggttagcg
    27421 gcagaccagt taactgacga catcgtggaa cagccggtca gcatcaacgg catagcgcat
    27481 aacattaaac gcagcgattt catgtcactt cctttggtta ttcaataacg ttgcttggag
    27541 tgcaaattca ccaaaaagtg ccgttattct ttttcttcat taaaacaagc gcgtaaacgt
    27601 cgattggtca gcagccagat taacgccaca atatccgcca ctaccagcgc cagaccgata
    27661 ccgctaacgg attcaccgtt cagccacagc cacggttgcc agcaaagtaa aaccacctgc
    27721 gccaacagca acagaaaata gagcacatgc caggtgcgag gaaacgaagc ccgccgaccg
    27781 ctcaacaaaa acgccagcac cgccggaata ccgggaatca gccccagcca gaaattatcg
    27841 tgatcgggat aaaacagatt tagcagcgca gtaccctgct cacgcgacgc accggcaatg
    27901 acaaacagca cccaggttcg cgcctgaagc aatagcacaa gccagaagag caagggtaaa
    27961 cgcaggcgtc cgtgcgcatc ataatggaca ggatggaact cagtactctt catcttcaat
    28021 caaacgctta cccagactca gcacgtcggc gtgttcatat cccaggcgtt catacattcc
    28081 gagcaccatg tcgttatctt ccggcacgtt gatctgaatt ttcgggcagc cgcgagcaat
    28141 cagctttttc tccagccggt taagcaacgc attggcaatt ccacgcccac gaaactctgg
    28201 atgcacgcca agataataag cagacccgcg atgcccgtca taaccgccca tcaccgttcc
    28261 gaccacttca ccgtttacct cagcgaccaa aaacaaactg acgtcatggt tcatcttacg
    28321 ctcgatgtcc atttccggat cgttccacgg acgcagcaag tcgcaacgct cccaaagggt
    28381 gatgacctct tcgaaatctt cctggcgaaa tacgcgtatc tccatggtat tcgttacctt
    28441 tttgcgggtt aaaaggctga ttatggcgtg aacggtcgaa ttagccaata tctgacgaaa
    28501 atcggttgaa aaagtggcat aatggggagt tgtcaactat tgaaatgaaa agtaaaacaa
    28561 ttctcaacag caaaccgtcg taacggatta cgcgatacga tataacatct ggaactttat
    28621 tattacaact caggccgtat gagcactttt aaaccactaa aaacactcac ttcgcgccgc
    28681 caggtgctga aagccggttt agctgccctg acgttgtcag gaatgtcgca agccattgcc
    28741 aaagacgaac ctttaaaaac cagcaacgga cacagcaagc cgaaagccaa aaaatctggc
    28801 ggcaaacgtg tcgttgttct cgatccaggc cacggcggaa ttgataccgg agcgatcgga
    28861 cgcaacggtt cgaaagaaaa acatgtggtg ctggcgattg ctaaaaacgt ccgttccatt
    28921 ttgcgtaatc atgggattga tgcgcgttta acgcgttctg gcgatacgtt tatcccactt
    28981 tacgatcgcg ttgaaatcgc ccataaacat ggcgcagatc tgtttatgtc aattcatgcc
    29041 gatggcttta ccaacccgaa agctgccggt gcttcggtat ttgccctctc taaccgtggg
    29101 gcaagtagcg caatggcgaa atacctgtct gaacgcgaaa accgcgccga tgaagttgcc
    29161 ggtaaaaagg cgactgacaa ggatcaccta ttgcagcaag tactgtttga tctggtgcaa
    29221 accgatacta ttaaaaacag cctgacgctt ggctcgcata ttctgaaaaa gattaagccg
    29281 gtgcataaac tgcacagccg caacaccgaa caagccgcgt ttgtggtgtt gaaatcgccg
    29341 tcggttcctt cggtgctggt ggaaacctcg tttatcacca acccggaaga agagcggctg
    29401 ttaggcacgg cggcgtttcg tcagaaaatc gccacagcga ttgctgaagg cgtgatcagt
    29461 tatttccact ggttcgacaa ccagaaagca cattcgaaaa agcgataagt tatgaaaccc
    29521 gacgcacacc aggttaaaca gtttctgctc aaccttcagg atacgatttg tcagcagctg
    29581 accgccgtcg atggcgcaga atttgtcgaa gatagttggc agcgcgaagc tggcggcggc
    29641 gggcgcagtc gggtgttgcg taatggtggt gttttcgaac aggcgggcgt caacttttct
    29701 cacgtccacg gtgaggcgat gcctgcttcc gccaccgctc atcgcccgga acttgccggg
    29761 cgcagtttcg aggcgatggg cgtttcactg gtagtgcatc cgcataaccc gtatgttccc
    29821 accagccacg cgaatgtgcg tttttttatt gccgaaaaac cgggtgcaga gcccgtttgg
    29881 tggtttggcg gcggcttcga tttaacccct ttctatggtt ttgaagaaga cgccattcac
    29941 tggcaccgca ccgcccgtga cctgtgccag ccatttggtg aagacgttta tccccgttac
    30001 aaaaagtggt gcgacgatta cttctacctc aaacatcgca acgaacagcg cggcattggc
    30061 gggctgttct ttgatgatct gaacacgcca gatttcgacc actgttttgc ctttatgcag
    30121 gcggtaggca aaggctacac cgacgcttat ttaccaattg tagagcgacg taaagcgatg
    30181 gcctacggcg agcgcgagcg caattttcag ctctaccgtc gcggtcgtta tgtcgagttc
    30241 aatctggtct gggatcgcgg cacgctgttt ggcctgcaaa ctggcgggcg caccgagtcg
    30301 atcctgatgt caatgccgcc actggtacgc tgggagtatg attatcagcc aaaagatggc
    30361 agcccagaag cggcgttaag tgagtttatt aaggtcaggg attgggtgta actccctcac
    30421 ccccattccc gcatccgctg atgcaacgtc aacgacggct tctcggcaaa cagctgctgg
    30481 taatccgtgg caaattgccc cagatgccag aatccccact gcatggcggc gtcttttacc
    30541 gttgtgcttt gcgaccacgg gcttatcagt tcgcggcgta cgacgttcag gcgaatgcgt
    30601 ttcagccacg cgtttgggcc aatgcctaaa atagcgtgaa acgcgttttg tagcgtgcgg
    30661 cgactgacat gcagttgatt acacaaatcc agcaccgtca ccggttcgga catgttttcc
    30721 agcacatatt cacgggcgcg ggaaagcaac cgacggtaac tctgatgact gatgctttcc
    30781 gccgtcacca ttggttgcgc ttcttccagc atcgccccca tcgccattag caaattatcc
    30841 cccagcactt ttcgcactgc aggctgatgg agattttccg ggttctcgca aaacgtcgcc
    30901 agcgcctgtt ggacaaagcc ccacagcgcg gctttatgct gctctttcac ttccagcgcc
    30961 gactggctac gcagcatatg taatacccga tccggattgt gcaaaaagtt agcctgacag
    31021 gtgatgacat cttcagacag cactacgccc aggatcgtat aatcgtccgg cgtacttaat
    31081 tcaaactcgg ttccaccggg gcgcgtcgct atttctgcgc ttcccagaca ttgcgaaccg
    31141 ataaatccct gctcaccgcg cgtcgccgga atgccaaacc agaacgagtt cggccagacc
    31201 aggcacgact gacgcagcgc cagaccggtg tattcacgaa aaacctgaat atcatcgagt
    31261 agaatttccg taaattcacc atgaaacttg cccggatgca gctgatcgta aatctgctgc
    31321 taggcggtaa tcgtcaaagc atgttcatag acatccgttg tctgtcgttg atgaacatta
    31381 tccacttcga ccttcggcgt gagcttcagg ttttcgggta agggttcatg ataaagatgg
    31441 tgcaaattgg ctgtacgggt ctttttcatg atgttaatgc cgggtgttgt aggacacccg
    31501 gcacctccga caggttaatg gggcttgaga cgataacgac tactgcgttt acgtaacgtc
    31561 ccggcagaaa agagctgttc gagcgcattt ctggcttttt ccagcggcca gccaaagtgg
    31621 gcggccactt ctcctgccgt cattccctga catactgatg ttaacagcgc caacaactcg
    31681 tcagcagatt ccgcgacgat aactggcgcg tcgggttcct ttacgggttg cttcggctgg
    31741 cggttaaagc cagtaaccag ccactgggtg tcatcgtctg gccgaccgat ctccttacgg
    31801 ctgatgacac gcccggtacg catcgcggca gcacaacctg cgtccagcgc cgcacgacac
    31861 gccgccagat cgccttccac caccagcgtt aagcgaccag ggtcaagcac ttcgtgactg
    31921 agcagacgaa cgttagctgc tttgagcatg gcatccgctg catctatcgc ggcgaccatg
    31981 ccgtccactt ccagcaatcc cagtgcattg atcatcggca acctccgtta cgcacgctgg
    32041 attggattac gcgcgatttc cagcactgca tcggtaaagg cgttacaggc tgctttacac
    32101 gcggcctggc taccggttaa aaatgctgcc gagtagttgg tttcagacgg tggcgggacg
    32161 taggtcacca gctgtacgtc ggcagatttc aacgcggcat caatgccgta ggtcgcttcc
    32221 agcggcggtg ccaccagata cgccatcgga tcgccaagcg tgatcccggc ggttgatgaa
    32281 agataagaac cggtacgcga aaccacatgc gccaggaagg cggtgttttc tgcgtcattg
    32341 gcccactgga aagctgcgcc attttcaata tgtgcaacca tcgcatccag accggcacgc
    32401 acttccgccg ggtttggccc accaagcata atcagcactt caccggcagt cggtgacggg
    32461 ccgtgtgccg ctcccgcata cagcgagcga ccatatacca cttcgaccat cgcttgtttg
    32521 gtcgcttcgt cagccgcaat gtaggttacg tcgtcagaat cggcagaaat cagcccgagg
    32581 ctacgaatat gcggcggcaa ttttagttca cgcgcaaatt cagcgttaac ggaggcaatc
    32641 acccgcatgg cggttaccga cggtcgaatc aaatctaaag ctggcatgat gcctccttat
    32701 cgggtcatgt tgatgccgga tgctttctgc tccagcatac gtttcgccaa atccacaatg
    32761 acggcacagc agcacgaaag agaagcaaaa gtcagctacc tggtacacta ggcagcgggt
    32821 cagcaaaggg gggcaaatgc cccttttttc atgtcagata atcagatcat tacctcaata
    32881 aactaataat cacatcgcgg ttacaaccag tttgatagtg atagctcaaa cccagacgtg
    32941 gcgctggctg caagcaaaat aagccgcaaa aatgcgaccc catgtttact agccgcaaaa
    33001 atgcggtcct atttgccaga accttttatt attcaattgg ttagtgcaag aaaaagcgtg
    33061 attactgttc aagaagtatc cagaaaggtc atgattttga cgctgaaaaa aaataggtgg
    33121 gcatttttgc ccactcacta gccgcaaatt tgcccactca ctagccgcat ttttgcggcc
    33181 caaagtaata aatacagaga aataataaat acagatcagc gccaagattt ttcgtctttt
    33241 cggcggaatc atgagactag tgcttacgtc ctgataacct ccagagcaga ccttaacttc
    33301 tgtgcagcag gactacgctc attgccgctc ggctaggtca aatcctcacg ataaagctgt
    33361 tcggattgtg accataatgt taaaggactc tttaaatcat tccttgacga attaactagc
    33421 tggcttaagc ggaatagcat gcaggataaa aacaaaacca gcttagcagt ggggagagct
    33481 tcgcttcgct cgtctcctgg ggccaatgct caatgaaacg acgtaaggca cggagcgctc
    33541 accataggta gacacataaa catctgagag cgcaccttct atcgcgctac gcgctcaata
    33601 aaaaccaacg acaccgccac actgcaagac caatggtttt tatcgcgtat acagaccaga
    33661 caggaaacag cagcaaagca gcgtatagcc aggtgcagac agcacactca cacgacgcac
    33721 agcaggctta agtgatcact caggaaactt tatccccgcc gccgatcacc gcgaactatc
    33781 atgaccgcgc caaattcgaa aggaatggcc catagaacgc gatgaacaca cataagcgct
    33841 acacttactt aacgtcgcca cagagcgatt gagagcgctt aaaatcgatg cgatttcatt
    33901 aaatttttct cactcgcctg aatggtggcg atcaggactg atcgcaacat tgccccgcct
    33961 cgatattgag cgcataatag atacatacat taagcggaaa agagataagt ttattgtgaa
    34021 aatcaggggg ttacatatca tgcgcatatc agaacaacaa cggcagatcc tcgactatct
    34081 ggcaaaaaac gaatcaatca ccgcacgaca agccgcagat ctcgtttatg gtggcaatgt
    34141 tacccgcgtc caggttgacg ccgcccgccg ctcactcatg acgatggtta gtaatgggct
    34201 aatcaggaaa caaggccgcg ctttcgtagc tgaaagacat atcaatcacg cttatagcca
    34261 ggacgttgaa gaattgcgcc gccagatcct ttgggtattc agcaaacagg gtcaggagta
    34321 tttcctttct gaacacccaa atttcgccgc caaagagtta acagtaagcg gattgtcgtg
    34381 cgcactggat tatcttgtcg gtgaaggttg cgttacttac aagcagctat ggcacgccct
    34441 gaatacgctt gtagaagatg gtttactcat tgccacgcgt gagaacatcg aatactggat
    34501 aatgccgcgt tcaggtcgcc gcaatcatgc acggtatatt tcaggcaaca gatacagaac
    34561 gccggacttt aatccgacac aggaagatcg ggaccgtgcc gaaatgatgt gcagggaaca
    34621 ggagaaaaga attatggata agttattttc gatgcagtga acacaagcgg gcgcaatgcc
    34681 cgtttttatt gccttattcc acgcctgacg aatcaatttg atcacacctg agaaaatgca
    34741 tagccagaag ttaaggccgt tatattgcga ttgggagcgt tttaaacggt atcaatttaa
    34801 ttaattctga tgtggtgatt tgcaggcaaa aaaaacccgc cggagaagcg ggaaatagag
    34861 acatatcgtt gtagttatta acagatcact ttgaggtcga gggcggctaa ccctcgctaa
    34921 tattatcccg ctattacctt gcgggtgcaa ataatctttt attgctttga tgatttaata
    34981 aatagcactt tttgctaaca aaaaccgctg tcaatatatg gccaaaaatg tcaacagaaa
    35041 aaataaggtt tataatatta agtatcacat gggtaatggg ttcagggaat cggaatccac
    35101 cgcctcacgg aaattcaaaa tttgcttctt ctttcctccg tgggcggctt ctttttcgta
    35161 tcgaggtatt tatgaaagca ataacattag aacagttcgc agcaatggcg aatgtatcga
    35221 ccagaacgct gaggcgctgg ctaaaaaaac catcaaccgg agtccctttg ccatttaccc
    35281 cacgcggtac tcgccaatta ttctgggagc acgaatgcaa agaatggttg tacagaacag
    35341 aacgataata atcacgcatc acattttgag gtaaacatca tgaccaatat cgaaagatta
    35401 gaacgctgtg ccgatattat gcgacgcaga tggatttatg accctgaagc gggtttattg
    35461 tttagccgtg aaacaaaaga cgttatcaaa ggcagcatga ccaataaagg gcatttggtt
    35521 gtaacggtac atgaaggtaa attcaaagtg acactcacct atcagaaagc ctgctatgta
    35581 tacacatacg gcgcttatga cgagacatta tatgaagtgc accacgttaa tttgaacaaa
    35641 caggataacc gcatcaagaa tattcgcctg atgccgaaag aaaagcaccg ccggatacat
    35701 aaaaacgtcc gcaaacttat tcggctgggc cacacacaat ggcttcaacc gcaattaatt
    35761 gaacaatcac tataaacaca ggagtaaata ccatgcattc attagacagt tattttcagc
    35821 gcacgacagc ccctaaatca gccgcccagg aacgccgtga ggaattccat gagaaagtga
    35881 tgcgttctgc tgattatatt gcggacaagt tcgtggaaac ggttcgtcca cttgttgatg
    35941 aagtagctga taaacttcag tctgaaatgc cggaagatat ggaaggcact gcaaagcggc
    36001 gactcatttg cgaattaagt cgtcgttttg gcgtgtcaat aagcgctttt aaataatcga
    36061 aaaaaggggg gaaagataat ggacgcattt gaggtactgg caaataaagg cgctgaactg
    36121 gtagcacagc gtgataaaac cgccaacgaa ggggaacgcg cactgctaaa taaacagatt
    36181 aaagcgatcc gtatggccca gtttaaatta atcagtaatg aagttatcga aacaattaaa
    36241 ccgcagatcc cacaaatcgt tgctgacgct attaaagccg ccggactgga gaaacgcatt
    36301 gccgggctaa aaacgaacgc agagaaaatg gagatgtaca aacagagcgg tgctccgaat
    36361 cccgatgagt acttctcacc ggatgttgag tttatggcgc aggttgagga acgtctgggt
    36421 agctgtctca ccgaggaaca gcgtcgctat tttgatggag tggatagttc ggcaggtatt
    36481 gacctgaact cgtatttcgg tcgtgaaatt gagcattttg atgccgcccc gtctatgccg
    36541 gagccagaac cagaaatggc caccgatcct gaagctgagc aacggaaacg cgtattcaat
    36601 gcgataggga ttcggtactg atcggataaa agaaagccgg gaattacccc ggctgactta
    36661 acaatgtgac caatgaacaa atcggacaag acaaacagga taagcagtct cgaaacaatg
    36721 ttcgggaact atattagcac aagtccggag aaaatcatca tgttggcact acgcatcatg
    36781 cagggcatcg ctaagaccct cgcagaacat gtcttagacc tgaaacattc gccactgagc
    36841 aaacaggcca tgaagcgcca aacgcttcgt ctgtgggctg aatattcgct tggaactatc
    36901 aataaaatca tcgacatgaa atcggggccg agcaaccaga gcgcggagga aatggagttc
    36961 atcaggcgtt taattctcat tcgcagagac attcatagcc agttacacag cgtgggaatc
    37021 gatattaatg atgggacggg cgattaattt tcggacaagt actatgcgcg cgcgaggagc
    37081 tgaacatgaa gcaagatatt gcagacaggc tggagatact cgaaggccag cgggcagaag
    37141 cgaaacaact acgtaagcag gccagaagag cgcacaggaa caacgaagca gagttactaa
    37201 ccaaatacat cagtttcacc aattactgca tctacgagtg ttacaaggaa gatgcagagg
    37261 actggctgga ttcattgcca gaacagtact gagcagaata acaccacgtc atgttgttct
    37321 gaggtgagca tatcacctct gttcaggtgg ccaaatttag tgtgccactt cagcatcacc
    37381 gttgaaatca caataacgat ttttttgtaa atatgctctc acatatgtag gaatagacca
    37441 ggttggcatt gaaacacatc taccatgacc ataataaagt aattttttga tatgtttatg
    37501 gttttatatt aattaaaata tatctaaatt tgatagtttt aaaattgcaa aatccagatt
    37561 gtgtttgtaa tgtttaatct gattgtattg agtggcgttt attatgaaaa ataaatcagg
    37621 taattcttgt tatagagaag cagtcgatat tcatagctca cttgagcttt catttatatt
    37681 gcataaatat catttgcgtg tagatgagaa gaaatctcga aatgtattca gcgccatacg
    37741 tgatgagctc atccttgatg gggattcacg acaaaactta gctactttct gtcaaacatg
    37801 gcttgatgat gaaatccgtg agttgcttaa tttagctatt gataaaaaca tgatttataa
    37861 agatgaatat ccacaaatgg cggaacctga aggtcgctgt gtgcgtatgc tttccgattc
    37921 gtggagtttc cccgattcgc gacacacctt aggttgctca accattggtt catctgaagc
    37981 tgctatgctc ggccgcttgg cgttaaaatg gcagtggtgt aaaaaacgcg aagtacaagg
    38041 taaatcaacc gaacctgatt tgcgatcctg tgcaaatatg ctggcataaa ttctgccgtt
    38101 actgtgatgt gaaattgtgt gaaattccac ttgatgatca tcacctgata atgaatgcta
    38161 aagaggtttt gaagcatgtc gatgaaaaca ctattggtgt cgttacgact ttaggtatta
    38221 cttatttttg taaagcagat tgcgttagtg tttttaagat aagtgttaaa gttaatatta
    38281 gtttttatat ttacatcgta attatatgtt tctattttaa aaatgttcga gagtataaaa
    38341 atgtctaatg ctaatatcgc acccaaaagt aagaaaatga cagtggggac actcgctatc
    38401 atgaatataa ctgctgtagt aagtctacga tttatacctt ctgaagcaga atacggctta
    38461 ggtgccattt tttactatac ctttgctgca attatgtttt tagtacctgt tgcctttgtt
    38521 gcggctgagc tagctacaac atatccacaa aaaggggggg catttcgatg gataggagaa
    38581 gcatttgggg ctcgctgggg ttttgtagga atgcttatga cctggctaca agtcatcccc
    38641 tactttccaa cagtactgac atttggtgca gtctcggttg catttattga tgttgatatg
    38701 ggggtggcag aatcaattgc cagtaataaa tggtatattt tatttttcgt actatttgtt
    38761 tactggggag ctatttgtgt tgcattacgt ggcgttggta ttttctcgca ggtatctaag
    38821 tggtgcggta ttatagggac aattattcca gctattatcc ttgttattct tggtttctcc
    38881 tatctgtggt tcagcggtaa accagcggtg atcccattag gttggggtga tcttttccct
    38941 aatatagcta acttccagaa tattgttctt gccgccagca tatttcttgc ttatgcaggg
    39001 atggaaatga atgcggtcca tgttaacgat ttagataatc caactaaaaa atacccaatt
    39061 gcaattacca tcgcttctat aggtactatt gcaatattcc tgctcagtac tttaggggtc
    39121 gcctttatta ttcctacaga taaaattagt ctaactcaaa gtttgttggt tgcctacgat
    39181 atgctgttcg aatgggctgg agtgccatgg cttggctctg tgatggcatt tatgctagca
    39241 attggcgtac tgggtggggt ggttacttgg attgcagggc ctaatacagg tgtattagcg
    39301 atcgcgaaag ctggttattt acctaagttt ttccaaaaaa ccaatcgtca tggaatgggg
    39361 caccatctaa tgtttgtaca aggtataatt gtctcagttc tttctgttac ttttgtgata
    39421 atgccttcag ttcaagcagc ttttcagatt ttatcacagt taacagttat gctctattta
    39481 gttatgtata tgctcatgtt cgctagtgct atctatctac gacattctca acctgataca
    39541 aaacgcccat atcgagcccc tgctttattt ttctgggcaa cacttggatt cttagggtct
    39601 tttctggctt ttgtattaag ttttattccg cccgagcaaa tcccaactgg tagtccaacc
    39661 agctatgttc tttatttgtt agtattagtt gccctatttt tattaattcc aatattaatt
    39721 tacgccttcc gaaaatctga ctggaaagat ccaaactctg atttcgaacc attttcttgg
    39781 gaaaaaagaa ctagtaatta aattttccat aagctacaaa agatgaggtt aaatatgttt
    39841 agtgctgaat ctccaattgg tgtaatagac tcaggtgtcg gtggattgac atcggtaaag
    39901 gaaataatta atttgcttcc tggtgaagat attatctatt gtggagataa tatgaatgca
    39961 ccttatggca accgttctgc cgatgatatc atcagtttga ctaaaaaaat gctgactttt
    40021 ttacagtcaa ggaatgtcaa actagttgct gtcgcatgta atactatatc ctcaactttg
    40081 gaatccgaag aatacagcgg atatgctaag agctttccgt ttcctatctt atccatcatt
    40141 gaacctgcag tagaagatgt gattcgccag caatataaaa atgttggtat tatcgctacc
    40201 gagtttacta ttaaaacggg gtgtcacaaa gagctaataa aaaaattgaa ttcaacaata
    40261 aatgtatttg gagaaccaag caaaaatctt gcaatgttaa ttgaggaagg taatcttaat
    40321 gctcctgcaa tcttgaatga catcaaaaaa catgttaacc atttaatttc cctccatcca
    40381 gtaaatgaaa ttattctcgg ctgtactcac tatccaattg ttcagaattt ttttgaagca
    40441 gttgcaccgg atataaaatt tattaatcct gcacatgatc aggctatctc tattaaaaat
    40501 cacttgggac agctaaattt attaaactcc tcaaatatag gaactttgca aataaatact
    40561 agtggaagta tggagatcta caaaacggta ctaggggaat tgagcattac gaaacctcat
    40621 acattcagta tcaggcagtt ttaaatttat ccggtacacc gtaaaatccc gtccttctga
    40681 actgcacccc aaaagttgga caccaactga gtaaggtgca ttttatggac aaacccagtt
    40741 acatactggg tagtgcatcc aattagtaga acatgtgttt ttcgataaac gctccgatca
    40801 ctttttcgtg gatctcaact gagcgtgaga agcagattgt tttacgaccc aaccgcttaa
    40861 tgcgggtgcg tagcgtcaga ttattacgct caatgcgttg ggtgaatatt ttgccggtca
    40921 gatgcttatt cttcggcacc tcccggccat agctgcccca gtcatcgctg gtcagcatgc
    40981 cgatgttgaa gggtgtaagc agtgccagta gctcccggca cgtttgatcg gttcggggac
    41041 caaaagtgta ggccagtaca ccgcctgttt tggtgttgta cgcgtacccg agccaatgtt
    41101 gccgggcttt actgccaacg tagctccatt gctcatcaag ctcgcagata aacgccacat
    41161 cagcatgggc aacaggcgaa gaagttattc gctttggcgc gagtttttta aagtccggat
    41221 gacggtgtta ctaccaattt tcagtgtcct ggcggtatcg cgaaccccgg caccattaaa
    41281 ggccatttca gttatcagct ctttcatacc cggcttacgt gcttgataag tataagtgaa
    41341 ctgaaacaca cggtggcagt cacggcagcg aagtctgtca cggcctttag ggttctgacc
    41401 atggcggtaa acctgagctg actgacaacg gggacaatga atattaacgc tggtcatgag
    41461 ataacctcaa aagcccgtat tatacatcag attcaactaa ttagaggcat cacccatcca
    41521 gcattataaa acatactaac aataactaaa ttataataat cacaacttac aatcaaataa
    41581 taaatgcata agattaacaa tgattttttc gggttatatt tatctaaaaa agcagaatat
    41641 tatgtaactt attattttct aagcgaaaat ttattgtgcg gtcagactaa caaacttgcg
    41701 taccacccga agaacatgcg aagatttaag tattctttct tttttggttt ttatttttca
    41761 ttttctcttt tcttctcttt ccggttctgt tctggttttg gttttttcct ttttattttt
    41821 attctttctt cagggttatg ctaaaacacc aaaacatttt tcaccacaaa agcatccaat
    41881 catcttgcca gaccaatcac cttaaccgga tacactgccg gatataatcc cactaacacc
    41941 cagacaccaa gcaccatacc tgataactac cgtattcagg aaggaaatgc gagtgatttc
    42001 accgtcaccg gcacaaccga tccgccaaaa agaggcgtac caatgtcgat atagtccccc
    42061 gcgcggacaa tcacttcgtc aatgactgcc aacgggagtt gttgtagctg tgggcgcaac
    42121 aacatgccca gagctttacc aaagtcctgc ccggccacca ccagcagggg atgcggattc
    42181 ggaaaacgcg cgacgaaatc gaccagcgcg ttgatgaccg tcagaaccgc ggcgtaacgc
    42241 acaggcagcg aggcgggaag cgccagcacg tacacgtcag ttttgggatc aagatccagc
    42301 tgaagcagcg cctgttgcca ggcactcacc agatccgttt catcaatcgg gatcgccacc
    42361 ggcaaattgc gcagcggcag ttgtacgccc tccagccaga ttgtgctgcc agagagcgaa
    42421 agggtatgtg cacccgcgcc aatcaccgtg gagcgtacgg tttgcgccgg aaactgcaca
    42481 ttcatctcac gcaggcgcgg atggtcatgc agcgccgttg ccagaagtgg gccaatatcg
    42541 gcaaaacaga acgggttggc gggctggtgg cgataacatt cgcccacgcc tccagaaagc
    42601 gtaatgattt cgggcgtaac acctgcgggc agcaaaccgg tttgcatcaa tgcctgcgcg
    42661 agcggcgaaa gcgttccgtc aatcacttcg acaatcagcg ccgccatccg ccgggtaacc
    42721 tgcaccagct gcgcgccggt cagcgaacgg gcgtcagtgc ctgcaccgaa gcactcatcc
    42781 acaatcatct gccccggttt atgagcgtaa accacgcgcc cctggctgtc ggtttccagc
    42841 aggcgaccac cgacgttgag acaggcagtg ccgctgattt ttccggcatc gaacagggcg
    42901 tagttcgcgg tgccaccgcc gatatcgata ttcagtaccc gacacagccg ttgttcagaa
    42961 agggtttgcg ccccggctcc gtgaccggcg atcacggatt cgaggtgcgg cccggcgctg
    43021 gcgacgacaa aatcgcccag cgactgtttt tccttcatca ctttttccat cagctgtgcg
    43081 accaggcttt cggtaaactg gccttccggc agctgggcaa tgatggtttc gcggatgcgc
    43141 tggctttcgg ttttgtcggc tggcacgctg gcgggcacgg acgccgttac cgggatcgat
    43201 tcatcacatt cggtgatggt aaaacccagt agatccgcca cttcacgggc ttccggggta
    43261 ataatgctgg cgcgcagaac cacggacatt gcctgttcgc cgcgcgcgtg tgcttcacga
    43321 atatcattcg ctgtgatgag ttttttcacc tgcttcctcc tgtttggtga gagatgccag
    43381 ataatccacc agttgctgca cgctttgcgg gtcgtggctg tttagctcga aaatcggctc
    43441 ttcgaaaccc gtctcaagca ataactttcg cgttgcggcg acgtcagcat ctggcatgtc
    43501 cgttttactg atgacggcga tttgccgttt gctgacgcca atatccagca acccggcagg
    43561 caaccgactt tccgggtcat ttgcgccatg gacatagatc agcatgtcta catcctgtag
    43621 cgtggtaatt aaggcgtgat accagcgggg atggctaaaa tattcacccg gcgtatcaat
    43681 atcgccctta tcattaaatt ccacggccag tgtttttctg gcgagggtat aattcccctg
    43741 caacgcatta aataacgttg tttttcctgc cccgacgcta cccacaaacg caattcgttt
    43801 catgaatgcc tcgtattaac ttttggtcat ttcacaaagc gtataattta ataagcgacc
    43861 taaaccgctg acggtttgtg ataacgcttc ttctactgcg cccaccgagc cataaatcac
    43921 caatgcaccg ctgaagcggt cgagaaaacc gatatgcaca tcagcggctt tcagcgctaa
    43981 atcaccggcg atcatcgccg tttcgccggg agttagcgtc ataatgccaa ttgcgcccgc
    44041 atccggaacg ccgattttct tcgccagttc ctcaccggga tgcgcgatga gatgcgccag
    44101 tgtgacctgt ttgccgggca caaattcctg aatgatgcgt tctttatcca tgagtcgccg
    44161 cctgtgaaaa actttcgtgt atcgtgacaa tttttcgtca gagtaaataa caaaattcgg
    44221 caagcgcgtt taaaaggtga ggtagatcaa taagaatgga gagatattta ctgttatgaa
    44281 taaactttca tatgcgacgc atgaatataa ataaaataat atcgagatat tttatatact
    44341 gattttttta ttattcgcaa actcagtaat aagctttgct aatatatttc aggaaaaccg
    44401 cttaccctga atattcaggg taagcgtgag agttaagaat gaattacagc ggttgggttt
    44461 gcgcttcaac cacggccagc gccaccatat tgacgatacg acgcaccgat gcgatcggcg
    44521 ttaacacgtg aaccggtttc gccacgccca tcagcaccgg accgacagtc acaccttccg
    44581 agctggaaac gcgcagtaag ttgtaactaa tgcgggcagc ttccatgttc ggcatcacca
    44641 gaatattggc agaacctttc aaagggctgt ccggcatacg gtcgttgcga atcgcttcca
    44701 ccagcgctgc atcgccgtgc atttcaccat caatcatcag atctggcgca cgatccctga
    44761 ccagttccag cgcctgacgc attttgctcg acgacgggca gtcagaagaa ccaaagttgg
    44821 agtgcgacaa cagagcaacg cgcggctcaa taccaaaacg acggacagtt tctgccgcca
    44881 tcaaggtgat ctccgccagc tcttctgcgt ctggttcgtc attgacgtag gtatcggcaa
    44941 taaaggtgtt accactcggc agcagcagcg cattcattgc acctgcggtg tgaacgccat
    45001 cgcgataacc aaagacattt ttcaccacac taaaatgttc atgataatcg cccaccgtac
    45061 cgcagatcat tgcatcggct tccccacgct gaaccatgat cgcgccgatc actgtcgggt
    45121 tactgatcag cgcacgctgc gcctgttcct gagtgacacc gcgacgcttc atgatctgga
    45181 agtattcggt ccagtactct ttaaagcgcg gatcggattc gttattgacg atttcaaaat
    45241 caacgcccgc tttgatctgc aagcccagtt tctgaatgcg catttcgatc acgttcggac
    45301 gaccgataag gatcggtttc gccagtccca gcgttaccag ttcctgagtc gcatgcagaa
    45361 cgcgcgcctc ttccccttcc ggcagaacta cgcgcttcgg cgctttgcga gcctgggaga
    45421 aaatcggctt cataaacagg ttggttttgt aaacgaactc agtcagcttg tcgatgtaga
    45481 cgtcgaaatc agcaatcgga cgcgtcgcca cgcccgactc catcgccgct tttgcgaccg
    45541 caggagcgat cttaacgatc aagcgcggat caaacggttt tggaatgatg tattccggac
    45601 caaagctcag atcctgaacg ccatacgctg aagccaccac ttcactctgt tctgcatggg
    45661 ccagttctgc aatcgcgcgt accgccgcca gtttcatctc ttcgttgatg gcggttgcgc
    45721 caacgtccag cgcgccacgg aagatgaacg ggaagcaaag aacgttgttc acctggttcg
    45781 ggtagtcgga acggccggta cagatgatgg catccggacg cacttctttc gccagcggcg
    45841 gcagaatttc cggttccggg ttcgccagcg ccaggatcat tggtgcccga gccattttct
    45901 tcaccatttc ctgggtcagc actttcgggc cggaacagcc caggaaaata tccgcacctt
    45961 caatcacatc atcgagggta cgtttgccgt catccaccac cgcatatgcg gctttggttt
    46021 ccgccatatt tggctcacgg ccctgataga taacgccttt tgaatcgcaa accacgatgt
    46081 tatgtttttg cagccccagc gctaccagca ggttcataca ggcgattgct gcggcacccg
    46141 cgccggaaac caccatccgc acgtcggaga tgtttttctc caccacgcgc aagccgttga
    46201 ggatggcggc agtgctgatg attgccgtgc cgtgctgatc gtcgtggaat accggaatat
    46261 tcatccgctc gcgcagtttc tgttcaatat agaaacattc tggcgcttta atgtcttcga
    46321 ggttgatgcc gccgaaggtc ggttcgagcg cggcgacgac ttcaataaat ttgtccgggt
    46381 cgagttcatc aacttcaatg tcaaatacat caatcccagc gaatttctta aatagaacgc
    46441 ccttgccttc catcaccggt ttgcctgcca gcgcgccaat gttgcctaac cccagcaccg
    46501 ccgtaccgtt agagatcacc gcaaccaggt taccgcgcgc ggtatatttg taggctttta
    46561 gcgggtcttt ttcgatttca agacaaggtg cggcaacgcc tggtgagtag gccagcgcca
    46621 gatcgcgctg cgttgccaga ggcttggttg gagaaacctg gattttccct ggaactggaa
    46681 attcatggaa atcaagtgca ctttgtttta actggtcatc catttggttg ttcctttcac
    46741 gtaacgttca caaataaagt gtgtgggcaa cagcccctgc ccacaacgtg gcgcacatta
    46801 ttaccctgcc ggagtctaca gactttgagc aagtccaaac tctcaccatt aatataatgt
    46861 tttggtaata atcctataac actgatgtta cctgcttaat ccagcaatac catgcctgtc
    46921 tgctatgctt ttttgatgcg tttagcgaaa tttctcagaa gtgtgaatta acgcactcat
    46981 ctaacacttt acttttcaag gagtatttcc tatgaacgag ttagacggca tcaaacagtt
    47041 caccactgtc gtggcagaca gctgcgatat tgagtccatt cgccattatc atccccagga
    47101 tgccaccact aatccttcgc tgttactcaa ggctgccgga ttatcacaat atgagcattt
    47161 aatagacgat gctatcgcct ggggtaaaaa aaatggcaag acccaggaac aacaggtggt
    47221 cgcagcgtgt gacaaactgg cggtcaattt cggtgctgaa attctcaaaa tcgtacccgg
    47281 ttgcgtgtca acagaagttg atgcacgcct ctcttttgat aaagaaaaga gtattgagaa
    47341 ggcgcgccat ctggtggact tgtatcagca acaaggcgtt gagaaatcac gcattttgat
    47401 caaactggct tcgacctggg aaggaattcg cgcggccgaa gagctggaaa aagaaggtat
    47461 taactgcaac ctgacgctgc tgttttcttt tacccaggcg cgggcctgtg cggaagcagg
    47521 cgtttttctg atttcgccgt ttgtagggcg tatttatgac tggtatcagg cacgcaagcc
    47581 gatggacccg tatgtggtgg aagaagatcc gggcgttaaa tcggtgcgca atatctacga
    47641 ctactataag caacaccact atgaaaccat tgtgatgggc gcgagcttcc gtcgcaccga
    47701 acaaatcctc gccttaaccg gctgcgatcg actgactatc gcaccgaatt tgctgaagga
    47761 gctgcaggaa aaagtttctc cagtggtacg taaattaatc ccgccttctc agacgttccc
    47821 acgcccagca cccatgagcg aagcggagtt ccgttgggag cacaatcagg atgcgatggc
    47881 ggtagaaaaa ctgtctgaag gcattcgtct gttcgctgtt gatcaacgca aactggaaga
    47941 tcttcttgcc gccaaactat aaaccagcca cggagtgtta tatgtcccga aaagaccttg
    48001 ccaatgcgat tcgcgcactc agtatggatg ctgtacaaaa agccaattct ggtcatcccg
    48061 gcgcgccgat gggcatggct gatattgccg aagtgctgtg gaacgatttt cttaaacata
    48121 atcctaccga cccaacctgg tatgatcgcg accgctttat tctttccaac ggtcacgcgt
    48181 cgatgctgct ctacagtttg ctacatctga ccggttacga cctgccgctg gaagaactga
    48241 agaacttccg tcagttgcat tcgaaaaccc caggccaccc ggagattggc tatacgccag
    48301 gcgttgaaac caccaccggc ccgcttggac aaggtttggc gaacgccgtc gggctggcga
    48361 tagcagagcg tacactggcg gcgcagttta accagccaga ccatgagatc gtcgatcact
    48421 tcacctatgt gtttatgggc gacggctgcc tgatggaagg tatttcccac gaagtctgtt
    48481 cgctggcagg cacgctggga ctaggcaagc tgattggttt ttacgatcac aacggtattt
    48541 ccatcgacgg tgaaacagaa ggctggttta ccgacgatac ggcaaaacgt tttgaagcct
    48601 atcactggca tgtgatccat gaaatcgacg gtcacgatcc gcaggcggtg aaggaagcga
    48661 tccttgaagc gcaaagcgtg aaagataagc cgtcgctgat tatctgccgt acggtgattg
    48721 gctttggttc gccgaataaa gcaggtaagg aagaggcgca cggcgcacca ctgggggaag
    48781 aagaagtggc gctggcacgg caaaaactgg gctggcacca tccgccattt gagatcccta
    48841 aagagattta tcacgcctgg gatgcccgtg aaaaaggcga aaaagcgcag cagagctgga
    48901 atgagaagtt tgccgcctat aaaaaggctc atccgcaact ggcagaagag tttccccgac
    48961 ggatgagcgg tggtttaccg aaggactggg agaaaacgac tcagaaatat atcaatgagt
    49021 tacaggcaaa tccggcgaaa atcgctaccc gtaaggcttc gcaaaatacg cttaacgctt
    49081 acgggccgat gctgcctgag ttgctcggcg gttcggcgga tctggctccc agcaacctga
    49141 ccatctggaa aggttctgtt tcgctgaagg aagatccggc gggcaactac attcactacg
    49201 gggtgcgtga atttggcatg accgctatcg ccaacggcat cgcgcaccac ggcggctttg
    49261 tgccgtatac cgcgacgttc ctgatgtttg ttgaatacgc ccgtaacgcc gcgcggatgg
    49321 cggcactgat gaaagcgcgg cagattatgg tttataccca cgactcaatt ggcctgggcg
    49381 aagatggtcc gacgcaccag gctgttgagc aactggccag cctgcgctta acgccaaatt
    49441 tcagcacctg gcgaccgtgc gatcagatgg aagcggcggt gggctggaag ctggcggttg
    49501 agcgccacaa cggaccgacg gcactgatcc tctcaaggca gaatctggcc caggtggaac
    49561 gtacgccgga tcaggttaaa gagattgctc gtggcggtta tgtgctgaaa gacagcggcg
    49621 gtaagccaga tattattctg attgccaccg gttcagagat ggaaattacc ctgcaagcgg
    49681 cagagaaatt agcaggagaa ggtcgcaatg tacgcgtagt ttccctgccc tcgaccgata
    49741 ttttcgacgc ccaggatgag gaatatcggg agtcggtgtt gccttctaac gttgcggctc
    49801 gcgtggcggt ggaagcaggt attgccgatt actggtacaa gtatgttggt ctgaaagggg
    49861 caattgtcgg gatgacgggt tacggggaat ctgctccggc ggataagctg ttcccgttct
    49921 ttggctttac cgccgagaat attgtggcaa aagcgcataa ggtgctggga gtgaaaggtg
    49981 cctgatggtg atcgccggat gctgtatgtt cccctcaccc taaccctctc cccagaaggg
    50041 cgaggggacg gtccgtgcgc tttttagggc cgcatctgcg attttgttta acgggtgatc
    50101 cacaacgtgg gcctggcgtc tggcccatgc cagttatcgc aggtaggttc tgcggcgtaa
    50161 cgcaccaggc gaaaacgctg accgtcaaag cgccagcgcg cctgaatgcc acaatcgctt
    50221 aatccgcgcc ctttcgctaa ggtcaccagt tcacgcgatt tctcatcaaa tgttgcgttc
    50281 atcagttcca gttcattcgt ctcctggccg ttgttgaacg gcaaacgcaa ccgaaccggg
    50341 cgcgaggcta gcggcttttt acgcgacaca atccatgcca aatcaatggt gttataggcc
    50401 cctgcctcac agctaatcat catcagtgct ttatcatcgg tcagcgcagt gacattcacc
    50461 tcacgacgta acggatcaag cgagcagcgc agaccattca tccgccagtt tccataatcc
    50521 agcaaatcgt tacgttcttc gagtgagagt ggcgtcggcg ttgggttaac caccgcgacc
    50581 tctttcagcg caggcgcggg cggtacgctg agaggcggtt cgtccccttt cttgatccac
    50641 gcggtttcac tgccaacgcg cttttgctga gcatcaataa acaacaacgc cgcttttaag
    50701 ccgctcagag aaatggtctg attgccgtcg cgtaaggtga tcgccttccc ttcctgaatt
    50761 atctgcaaaa acgcggtgat ggttgccgta tcatcggtta ctaataacca tggtgaaatc
    50821 cgccacttgt caccacttaa tgccaacggc tcaccatcta atagcagccg tggcgctatc
    50881 tccccttcag acgcatccgg cgacttcaac ccaccgcgct caatacgtaa aacggcatcg
    50941 gtatgcgccc cagcgctgcg gctcagggtc atcaccagtc cattatgatc gcccgtattg
    51001 cgcgccacac aaaaattttg attattacag gtgacctgcc agtcggaaaa cgcccgttgc
    51061 gctggtgccg cccacaccaa agacgtcggc aacaaagcga aaaaaaagag aaggaaaatg
    51121 cgatagcgca tggacggtac gaccccagaa gttcaaacaa acagcacaag tcgtatcttc
    51181 gttgcgcagg tagcgcggct caatcggatt tgtcggattg agccaataat aaacaacgaa
    51241 atactattgc tatttttcag tccattaaat gtgacatttg caaatagtta agcaataaca
    51301 ccgtcttacc gtcacgtatc tcgccggttt tgatcatctc cagcgcctgg ctgaacggca
    51361 gctcgagcac ttcaatatct tcatcttcaa caccgccacc ggcgttagcg cgctgattgt
    51421 cactgtattc ggcgataaaa aagtggatta gctcagtcac accgccaggc gacatataca
    51481 gttcaaataa tttgcgcact tcgcccactt catagcccgt ctcttcaatc gcttctttac
    51541 gaatgcacac ttccggttcg tcgttatcca gcagcccggc gcaggtttca atcagctgcc
    51601 cgctttcatt gccattaacc caggtagcga cacggaactg acgaatcaga accacgctct
    51661 ttttcttcgc gttgtacagg aggatcgtcg caccattacc gcgatcgtaa acttcacgtt
    51721 tatggcggat aacttcgcca tctttgcgcg tgagatcgta agtaatgttg tgcagggtga
    51781 aatagttatc ggagagaatt ttgtctttaa tgagggtgat ttgttgcgtc ataccgactc
    51841 cacagcgcga aatgaacaat tatattacgc tgtgaagtcg ggtttgtctg cgcaggctat
    51901 cagtgagatt tgactgattt tacccccagc cagtcaataa tcccctgtgc cgcatgacgt
    51961 ccttctgcca ttgcggtaac caccagatcc gcaccacgca cggcgtcgcc accggcgaag
    52021 attttcggat tggtggtctg gtaacggtac tggctttcca catccgcgat gatgcggccc
    52081 catttgtcta ccgttacacc gtgcgactcc agccacggca tcccgtgtgg attgaagcca
    52141 aacgccataa tcaccgcatc ggctggcatg acaaattcac tgccctcaat cggtacaggt
    52201 cgccgacgcc cctgggcatc cggctcgcca agacgcgtgc gcaggaaacg aattccgcaa
    52261 acgtgacctt gctcattcag ctcaagcgcc accggctgga cgttaaattc gaagttggcc
    52321 ccctcttcgc gggcgttctt cacctctttc ttcgagcctg gcatgttagc ttcatcacga
    52381 cgataagcgc aggtgacgtt actcgcgccg tggcgcagcg cagtacgcac acagtccatc
    52441 gcggtgtcgc cgccgcccag taccacgacg ttaagtccgg cggtattgat aaacggctct
    52501 tccggtagct cttcgagccc catcacctgt ttagtgttgg caatgaggaa cggcagcgcg
    52561 tcataaacgc ccggcgcatc ttcattgggt aaacccgctt tcatggaacg gtaagtgcct
    52621 acgccaacga agaccgcgtc gtattgttcc aaaagcaaat ccaaagagac atctttaccc
    52681 acttcacaat tgagttcgaa gtgaatcccc atcgcgctga agatttcccg acggcgtgcc
    52741 agcagggatt tatccagttt gaaagaagga atgccgaaag tgagcaagcc accgatttct
    52801 ggatggcgat cgtacaccgt caccgccacg ccattgcggg tcagaacatc cgcacaggcc
    52861 agccctgccg gacctgcccc gataatcgcc acccgcttgt ccactttggt gacatgactt
    52921 aagtcaggac gccaaccttt cgccaacgcc tgatctgaaa tatagcgttc aatgttgccg
    52981 atagttaccg cgccgtgctc atcgcgaata gtacaggcac cttcacacaa acggtcttgc
    53041 ggacaaacgc gtccggtaat ttccggcagg gtgttggtct ggtgagaaag ctcgacggcg
    53101 gcgtcgatgt ttccggcttt caccagttca atccactgcg gaatatggtt atgcagcggg
    53161 caggtccatt cacaaacgct gtgctcgccg aacttaaggc agcgcgaggc ttcccgttgt
    53221 gcctggtcgg cgcgaaatgg cagataaatt tcatcaaaac cggttttgcg cgcttcaatc
    53281 gccagtttat ccggctcgcc acgcgcgggc gttgcctgca tttgttcgac tttactcatt
    53341 accggcattt cttgtgcagc ggtactggca tgccacggtt gatgctcctg acgtgcggtg
    53401 cgcaagcggc gggatttcgc catgccggag agtgcgacgt cagtgaccag ttgcagcgcg
    53461 tccgccgggc aattctcgac acaggcagga ccgttttcgc gccctgcaca aaggtcgcat
    53521 ttatgcgccg tggcttttac ttttcctgcc gcgacgggtg tcaggacgat ttgcatcgta
    53581 ccaaacggac aggccaccac gcaggattta cagccaatac acttttgctg attgacctga
    53641 atgctgtcat caacgtggct gattgcgcca ttagggcagc tacgggcgca gggcgcatct
    53701 tcacaatggt gacaggtcac tgcactacgt tgctgctgat gtttgataac cgtaattcgg
    53761 ggatgaaaat ggtgttggct caggacatgt tgctcatcat tgtgagccat gacacaggcg
    53821 atttcacaag catgacaacc cagacattgc tgactgttgg ccataataaa acgattcata
    53881 acgaccttct tttttggttg taaaaacctt attctttata tgagtgttgt tattacccga
    53941 cttacagggg aaccggcaat gttcatgtgc ccagaataag taactatttc gctataaact
    54001 gtggcagatc aaataatccc atcactgact aaattgcgtt tcagcgaact ggaacattaa
    54061 tgattttttg tggagaagac gcgtgtgatt gttaaacgac ccgtctcggc cagtctggcc
    54121 cgggcctttt tttacattgt gctgctgtcg attctttcca cgggtatcgc tctgctaact
    54181 ctggcgagca gtttgcgcga cgctgaggct atcaatattg ccggatcgct gcgtatgcag
    54241 agttaccgcc tgggctacga cttgcaaagt ggcagtccac aactcaatgc acatcgccag
    54301 ttgtttcagc aggcactgca ttcaccggta ttaaccaacc tcaacgtctg gtatgtgcca
    54361 gaagcagtaa aaactcgcta tgcgcatctg aatgccaact ggctggagat gaataatcgg
    54421 ctcagcaagg gcgatttgcc gtggtatcag gccaatatta ataattatgt taatcagata
    54481 gacctgttcg tgctggcttt acagcactac gctgaacgca aaatgctgct ggtggtggcg
    54541 atttccctgg ctggcggtat cggtattttc acgctggtct tttttacttt gcgccgcata
    54601 cgccatcagg tggttgcccc gctgaatcag ctggttaccg ccagtcagcg tattgaacac
    54661 gggcagttcg actcgccgcc gctggatacc agtctgccga atgagcttgg tctgcttgca
    54721 aaaaccttta accaaatgtc gagcgagctg cataaattgt acctttcgct ggaggcgtca
    54781 gtagaagaaa agacccgcga tctccacgag accaagcgtc gtctggaggt gttgtatcag
    54841 tgcagtgttc ccaggcgctg aacaccagcc agattgatgt gcattgtttc cgccatattt
    54901 tgcagattgt tcgcgacaat gaagcagctg aatatctgga gctgaacgtt ggggataact
    54961 ggcggattag cgaagggaag ccaaaccctg agctgccaat gcagatttta ccggtgacaa
    55021 tgcaagagac ggtttacggc gaactgcact ggcaaaatag tcacgtttca tcatcagaac
    55081 cgctgcttaa cagtgtttca tcgatgctgg gacgcggttt gtactttaat caggcgcaga
    55141 agcattttca gtagttattg ttgatggaag aacgtgcgac catcgcccgc gaattgcacg
    55201 actcgctggc tcaggtgctt tcttacttac gtatccagtt gacgttactg aagcgttcga
    55261 taccggaaga taacgccacc gcacaaagta tcatggccga tttttcccag gcgttgaatg
    55321 atgcttatcg gcagttacgc gagctgttga ctactttccg cctgacgctg cagcaggcgg
    55381 atctcccctc cgcgttgagg gaaatgctgg atacgttaca aaatcaaacc agcgctaaac
    55441 tgaccctcga ctgccgtctg ccaaccctgg cactggatgc gcaaatgcag gtgcatttgt
    55501 tgcaaattat tcgcgaagcg gtgctgaatg cgatgaagca cgccaacgcc agcgaaatcg
    55561 ccgtcagttg cgtcaccgcg ccggacggca atcacacggt ttatatccgt gataacggga
    55621 ttggtatcgg tgaaccgaaa gaacccgaag gtcattatgg actgaatatc atgcgcgaac
    55681 gcgcggaacg gctgggcggg acgctgactt tttcacaacc ttccggcggc ggcacgttag
    55741 tgagtattag ctttcgctcc gcagagggtg aggggaaggg gaacacgccg gctgaggtgt
    55801 aaataattac cctcacaccg gccctctccc tttaaaggcg agagggaaaa acgtgcccat
    55861 attgacctgt aagcgagtgt gtcagcaatc tcatgataat ttacattaac tccttttttt
    55921 ctccacgatt ggctcgtacc ttgccgctac agtgaagcaa gtcaagccta caacaatacg
    55981 cagaaacacg aggtcctctt ttaatggcga atttctttat tgatcgcccc atttttgcct
    56041 gggtgctgac aatcctgttg tgtctgacag gtaccctggc gattttttca ttgcccgttg
    56101 aacaataccc cgatctcgcg ccaccgaatg tgcgagtgac cgctaactat cccggcgcat
    56161 cggcccagac gctggaaaac accgtgaccc aggttatcga gcaaaatatg accggcctcg
    56221 ataatctgat gtatatgtca tctcagagca gtggcaccgg tcaggcatct gtcactttaa
    56281 gttttaaagc aggcaccgat ccggacgaag ccgtgcagca agtacaaaac cagctgcaat
    56341 cagccatgcg aaagttaccg caggcggtgc aaaatcaggg cgtgacggtg cgtaaaaccg
    56401 gcgataccaa cattctgacc attgccttcg tctctaccga tggctcgatg gataaacagg
    56461 atattgctga ttatgttgcc agtaatattc aggacccgtt aagccgcgtg aatggcgtcg
    56521 gggatatcga tgcctatggt tcgcaatatt ccatgcgtat ctggctggac ccggcgaaac
    56581 tcaacagttt ccagatgacg gctaaagatg tcactgatgc cattgagtca cagaacgcgc
    56641 agattgcggt tgggcaactt ggtggtacac cttccgtcga taagcaggcg ctcaacgcca
    56701 ccattaacgc ccagtcactg ctgcaaacac cagaacagtt ccgcgatatc accttgcggg
    56761 tgaatcagga cggctcagag gtaaggctgg gcgatgtcgc caccgtcgaa atgggggcgg
    56821 agaaatacga ttatcttagc cgcttcaatg gtaagccagc ctccgggttg ggggtaaaac
    56881 tggcctccag cgcaaacgaa atggcgacag cggagctggt gctcaatcgt ctcgacgagc
    56941 tggcgcagta tttcccgcac ggactggaat acaaggtggc gtatgaaacc acctcgtttg
    57001 ttaaagcctc cattgaagac gtggtgaaaa cgctgctgga agctatcgct ctggttttcc
    57061 tcgttatgta tctgttcctg caaaacttcc gcgccacgct gatacccact atcgccgtgc
    57121 cggtggtgtt gatgggaacc ttctccgtac tttacgcctt cggttacagc gtcaacacct
    57181 taaccatgtt cgcgatggtg ctggcgatcg gtctgctggt ggatgacgcc atcgtggtgg
    57241 tggaaaacgt cgaacgtatt atgagtgagg aaggactcac tcctcgcgaa gccacacgta
    57301 aatcgatggg gcagatccag ggggcactgg tcgggattgc gatggtgctt tcggcggtat
    57361 ttgtaccaat ggccttcttc ggcggcacca ccggtgccat ctatcgccag ttctctatta
    57421 ccattgttgc ggcgatggtg ctgtcagtac tggtagcgat gctcctcact ccggctctgt
    57481 gtgccacgct acttaagcca ctgaaaaaag gtgagcatca cgggcaaaaa ggtttttttg
    57541 cctggtttaa ccagatgttt aaccgcaacg ccgaacgcta cgaaaaaggg gtggcgaaaa
    57601 ttctccaccg tagcctgcgc tggattgtga tttatgtcct gctgcttggc ggcatggtgt
    57661 tcctgttcct gcgtttgccg acgtcgttct taccgctgga agaccggggc atgtttacta
    57721 cctcggtaca attgcccagc ggttcaaccc aacaacagac cctgaaagtc gttgagcaaa
    57781 tcgagaaata ttacttcacc catgaaaaag acaacatcat gtcggtgttt gccaccgttg
    57841 gttctggccc tgggggtaac gggcaaaacg tggcgagaat gtttatccgc ctgaaagact
    57901 ggagcgaacg cgacagtaag accggcacct cgtttgccat tatcgagcgt gcaacgaagg
    57961 cgtttaacca aattaaagaa gctcgcgtta tcgccagcag cccaccagca attagcggtc
    58021 ttggtagttc tgcaggtttt gatatggagt tgcaggacca cgctggagcg ggtcacgatg
    58081 cgctgatggc agcacgtaat cagttgctgg cgctggcggc ggaaaacccg gagctaaccc
    58141 gtgtgcgcca taacggcctc gatgacagtc cgcagttgca gattgatatc gaccagcgta
    58201 aagctcaggc gctgggcgtt gatatcgacg atattaacga cacactgcaa accgcctggg
    58261 gttcgagcta tgtgaatgac tttatggatc gcggtcgcgt gaagaaagtc tatgtgcagg
    58321 cagctgcgcc gtatcgcatg ctgccagatg acatcaatct ctggtatgtc cgaaataaag
    58381 atggcggcat ggtgcccttc tctgctttcg cgacctcacg ctgggaaaca ggctcgccgc
    58441 gtctggaacg ctataacggt tattctgcgg ttgagattgt tggggaagcc gcaccggggg
    58501 tcagtaccgg tacggcgatg gatattatgg aatcgttagt gaagcagctg ccaaacggct
    58561 ttggtctgga gtggacggcg atgtcgtatc aggagcggct ttccggcgcg caggctccgg
    58621 cgctgtacgc catttccttg ctggtggtat tcctgtgtct ggcggcattg tatgaaagct
    58681 ggtcggtacc gttctcggta atgctggtcg tgccgctggg ggtaatcggc gcgctgctgg
    58741 caacctggat gcgcgggctg gaaaacgacg tttacttcca ggtgggcctg ttaacggtca
    58801 ttggtttatc ggcgaaaaac gccatcctga tcgtcgagtt tgctaacgag atgaaccaaa
    58861 aaggccatga cctgtttgaa gcgacgctcc acgcctgccg tcagcgttta cgcccgattc
    58921 tgatgacctc gctggcattt atcttcggcg tattgccaat ggcaaccagc acgggtgccg
    58981 gttccggtgg tcagcatgcg gtgggtactg gcgtaatggg cgggatgatt tcagctacta
    59041 ttctggctat ttacttcgtg ccgctgttct ttgtgctggt gcgccgccgc ttcccgctga
    59101 agccgcgccc ggaataagca ataaaaaagg cgacatgcca atgtgtcgcc tttttcaact
    59161 ttccgattaa gaacctgctc agcgggttct tgctgtttat actttgtctc aggaattact
    59221 tacgaagcat aacttcgata aagtctttcc agttccccag ttcacgttca atcataacaa
    59281 cctctcttat aattatgggt attctacgga aacaatatac cgtggtgaag ctaatttact
    59341 cgattgctgc gatgactacc tccgggggac aaaccttatg taaatactat ggtcctgcgg
    59401 tgataatttg tatgtgatac acagcaacat ttcgagatat tcatacggca tctaatactg
    59461 atttaattct ggttaaaata catacagata acaagatgaa tattcttaat gtttacgtta
    59521 aaaatgttta atattattta atagttgtta atttgaatac ttcgataatg ttatattttc
    59581 tgataatcat ttgcaggcaa aatgttttca cccttaaatg agtatttatt ctcataaatc
    59641 gaaaaaggat tcattatggt tacactttac ggcatcaaaa attgtgacac cattaaaaag
    59701 gctcgccgtt ggctggaagc caataacatc gactatcgtt ttcatgatta ccgcgtcgat
    59761 gggctggaca gcgaattatt gaacggtttt atcaacgaat taggctggga agcgttactc
    59821 aacacccgtg gtacaacctg gcgtaaactg gacgaaacta cccgcaataa aatcaccgat
    59881 gcggcctctg cggcggcatt aatgactgaa atgcctgcaa ttatcaaacg tccattgctc
    59941 tgcgcgcctg gtaagcctat gctgctgggt ttcagtgatt ccagttatca gcaatttttc
    60001 catgaggtgt agtctatgtc gtgcccggtt attgagctga cacaacagct tattcgccgc
    60061 ccttccctga gtcctgatga cgcaggatgt caggctttgt tgattgaacg tttgcaggcg
    60121 atcggtttta ccgttgaacg catggatttt gccgatacgc agaatttttg ggcatggcgt
    60181 gggcaaggtg aaacgttagc ctttgccggg cataccgacg tggtgccgcc tggcgacgcc
    60241 gatcgttgga tcaatccgcc gtttgaaccc accattcgtg acggcatgtt attcgggcgc
    60301 ggtgcggcag atatgaaagg ctcgctggcg gcgatggtgg tagcagcaga acgttttgtc
    60361 gcacaacatc ccaaccatac ggggcgactg gcatttctga tcacttctga tgaagaagcc
    60421 agtgcccaca acggtacggt aaaagtcgtc gaggcgttaa tggcacgtaa tgagcgtctc
    60481 gattactgcc tggtcggcga accgtcgagt atcgaagtgg taggtgatgt ggtgaaaaat
    60541 ggtcgtcgcg gatcgttaac ctgcaacctc accattcatg gcgttcaggg gcatgttgcc
    60601 tacccacatc tggctgacaa tccggtacat cgcgcagcac ctttccttaa tgaattagtg
    60661 gctattgagt gggatcaggg caatgaattc ttcccggcga ccagtatgca gattgccaat
    60721 attcaggcgg gaacgggcag taacaatgtt attccgggtg aactgtttgt gcagtttaac
    60781 ttccgcttca gcaccgaact gactgatgag atgatcaaag cgcaggtact tgccctgctt
    60841 gaaaaacatc aactgcgcta tacggtggat tggtggcttt ccgggcagcc atttttgacc
    60901 gcgcgcggta aactggtgga tgcggtcgtt aacgcggttg agcactataa tgaaattaaa
    60961 ccgcagctac tgaccacagg cggaacgtcc gacgggcgct ttattgctcg catgggggcg
    61021 caggtggtgg aactcgggcc ggtcaatgcc actattcata aaattaatga atgtgtgaac
    61081 gctgccgacc tgcagctact tgcccgtatg tatcaacgta tcatggaaca gctcgtcgcg
    61141 tgatgagtgg ttctgcaaga ggaaataagc atggactggc tggctaaata ttggtggatt
    61201 ctggtgattg tctttttggt aggcgtcctg ctgaacgtga ttaaagatct caagcgcgtc
    61261 gaccataaga aatttctcgc caacaagccg gagcttcccc cgcatcgtga tttcaacgat
    61321 aagtgggacg atgacgacga ctggccgaaa aaggatcaac cgaagaagta agctgagtgc
    61381 tcaaccgtag gccggataag gcgttaacgc cgcatccggc aatggtgaac gatccctgat
    61441 gcgacgctca cgcgtcttat caggccaggt aatctgtctt ttacatcatt tcaatcacat
    61501 catcatcgcc aggcttaccg ccacttagcg cttggtcgaa ataatgtttc ggaatggtat
    61561 aacgcaagtg atccagggca aattgcatac tgcgattgtc gatcgcatgg cctaaatcct
    61621 ccacgatatc cagcgtcacg tcaccaccgg cactgattaa cgcttcctgc gccgcaactg
    61681 catgcgccag atcgataacc ggatcttcac caccatgaat caagtgaatt gtcgtggctg
    61741 tagacgcggt ctccggcagg ctcgcatagc gtccgttaaa ggcaatgacg cgcgaagcaa
    61801 ggcccggttc ggctttaatg ctctccagcg ccataatcgc gccttgtgag aaaccaatta
    61861 gcgccgtggc gttagcccct actccgctct gtttctgcca gtagcgtacc gtttcaataa
    61921 acgtcggcat gatggcatcc acacgcgcct ggcgattatc ttccgtaata ccctgcactg
    61981 aaaaccactg acgccccgcc ggattaccgc tcggctccgc gccaccgaca ctcaccacca
    62041 gcgcatcagg aaacagcggt gcaaaccagc tgcctatttc ccccattgcc accgggttat
    62101 ccccgacacc gtggaaaagc agtaacaact gttgtgcagg tttatccggg ctttgaacaa
    62161 caaaatggtc atgtttcatg gcgaactcct taactgatgt cattaatttt acgccgctga
    62221 ctcacaatga ccatgggaga aaattgaagg agtcagtgaa aaaattgcca ttgcgttatg
    62281 cgatcgcgca gacgctcagt gcgaacctca tttagtgcga acagcgcctg cgcggcctct
    62341 tcccgctggc gaaccagtaa catcttgcgg cctgaaagtt taagtgtggt acataactgc
    62401 gcgtcactgg cgtttttctg taaacgccca cgcagcgccg gtaatgccag ttcactggtt
    62461 tgtagcagac gcaataagca acctaacgat gttaatagcg gacgatgagc gaaagcaaaa
    62521 ccggccagtt caagccagtc gtcgtcagaa aggatggcat cgtttagtgg atcaacagga
    62581 agcatttcgc cattccactg cgcgagagct tgcgcatcgc gacgtaaacg gtaatgctca
    62641 cgttcagcca gctgtttacc cgcatcactc atcggtaaca gcgccatcgc cgtatagcaa
    62701 ccgctgctgg cttcccgatg attacccatc cgcaccagca caaaaccgca gcgttgccag
    62761 aaacgccata actccccagt gtaaccaaaa ctcaccgaaa gatagtcgag gtcatgcgta
    62821 tattgcaaag ctccagcaat aagttgtcgc cctgtgcctt cccgctgacg agccggatga
    62881 actgctatcc ggctaacccg ccttccgcgc aatgtcgccg ccagtggatt gctgccgtgc
    62941 gccgccagcg actgggccac cagattaccc cgcgggcgac gaaaacctgc ccataccgcc
    63001 tgactgagtt cttgagataa tccgccctca tccaccagcc acaacgcccc ggcaatctcg
    63061 ttttcgccag ccgcctgtaa aaaatgttgc cctggtgcat ccatcatccg gcgtaaatcc
    63121 agcggcgagg tccggtagtg cgcaccagac aataactgat aaacctttaa cggcgtttct
    63181 ggctcgcttc gccataacgt ttgttcaaat gcggaaatga cgatattgcc ttgtggtgta
    63241 tgggtgaagt tttcatcgtt aaaaaccagt gcctcactaa ccattttttc cagcgggcat
    63301 ccctgtgccc agcggatcgg ctgttgcagt tcaaaacggt gtaaatgcgg aaagcgagcg
    63361 caaaatttca gcaaaaaacc acgtccggta ccttcgtagc cctgcaccgt agtggttaac
    63421 aacgttcgag gaaaacgcga taccagttga tgcaacaacg gcgcaggtat ggctgcggct
    63481 tcgtcgacca ccagccagtc ggcttgctca tcgctggcta acaaggcatc cggtgcaata
    63541 aagcgaaact tctcgcccgc aaattgtgcc agtacatccg ttgccgcttt tgcgggcgcg
    63601 gtgacaatcg cactgcccgc aatacgagaa atgagttgcc ctgccaacgc cgacttaccg
    63661 cgcccacgcg cagccgttac cgctgccacg cccggcggca tggtcagtag ctgctgtaag
    63721 agttgctgtt gttctggttg tggtgcgccg gtcgcggggt gccagtcagt acggggagta
    63781 aaatgcgcca acgagaacgg ctggttttgc cgccagagga tagcgtcgtt atccgccgta
    63841 agtacgcgtt tgagatgctg gacaaaatgc ggcgtcgcaa tagggtcagg gcaatcactc
    63901 cagcgcagcg agtcggcatc aggttggttt tcccactctt cccatacagg gagtaacaaa
    63961 accagccagc ttcccgcttt caacgttccg ctaagtgctg caaaggcagc ggcatcaaag
    64021 ccgtggcggg cgtcgaatac cgcatgccgg aactcgcgcc caagtaaagt ttgtagcgcc
    64081 gagggtgaac agtggttttc agcatctagc tgcggcgaaa tccacagcca gtcgccaggt
    64141 aaggcatcac gcaacttaag agcatgatca aaacaccaac cctcttcccc gctcaacacc
    64201 agcaagcggc agatcccttc acgtttcatt tgcgctgtta atgtgtaaag cgcagtcagt
    64261 tcagccatcc ctgccccgga aattaaatgc ttttaccaaa agtattgcat tgtgccggat
    64321 cgccgctgtc gaaaccacgt ttaaaccagc tgtagcgttg ctgagaagtg ccatgtgtga
    64381 aactgtctgg tactactcgc ccctgacttt gctgttgtaa acggtcatcg ccgatggcct
    64441 gcgccgcatt cagcgcctct tccagatcgc cagtttccag aacgccttgc tgctgcatac
    64501 tatgccccca gacaccggca aaacagtcgg cctggagttc catacgcaca gataagcggt
    64561 ttacttccgc ctgcgtcgcg ttttgttgca gttgacgaac tttcggctcg atgcctaaca
    64621 gtttctgcac atgatgaccg acttcatggg cgataacgta cccctgggca aaatcgccat
    64681 ccgcgccaag tttgtctttc atgtcatcat agaaggagag atcgatataa actgtgccat
    64741 ccgccgggca atagaacggc cccataatgg actggcccgc cccgcagccg gtgcgcgtca
    64801 ttccacggta catgaccagc ttcggttgct gataggtctt acccatcttt tcgaactgtt
    64861 gtccccaggt gtcttccgtg gttgcctgaa tcaccgaggt gaattttgcg gcttcgtctt
    64921 cattagggct aattgaccgc gttgattgtt gttgagaaac cggctgcccg gtcatcaacc
    64981 cggttaaatc aacgccatag tagcctgcaa ccagcacgac tatcagtaaa atcagcccgc
    65041 ctttaccgct cggcagacgg aaaccgggac cgcccattga cggaccgcca gagctgttgc
    65101 gcctgtcttc aacattgtca ctttcacgtc gcccttgcca acgcatagat acctcaacaa
    65161 tatattcatt atagaaatga tcgtaggtgg ttcagaggaa gattaccaca ggaaagcggg
    65221 atgaaatggc ggaggaatga gcgttcaatg catatattcc gagagcggtt gtcttgccgt
    65281 gccagctgca cggcaagatg atgaacagaa aaatcagtcc agctgtacac ccaggcggcg
    65341 ggcgacggct tcataggctt cgatcaggcc accgaggctc tggcggaaac ggtctttgtc
    65401 cattttctcc agtgtttctt tgtcccacag gcggctgccg tccggggaga actcatcgcc
    65461 cagtaccact tcacctttgt acagaccaaa ctccagcttg aagtcgacca gaatcagacc
    65521 agcatcatcg aacagttttt tcagcacgtc gttcgctttg taggtcagct ctttcatacg
    65581 cgccaggttc tctttgctca cccagccaaa ggtttcgcag taagattcgt tgaccatcgg
    65641 atcgtgcatg gcatcgtttt tcaggaacag atcgaacagc ggcgggttca gctcaatacc
    65701 ttcttcgatt ccaagacgtt tcactagaga gccagcagca cggttacgca cgacacactc
    65761 aaccggcacc atatccagct ttttcaccag acattcggta tcggagagca gacgctccat
    65821 ttgagtcggg atacccgctt cagccagttt gctcataatg aagtagttga acttgttgtt
    65881 caccatacct ttgcgatcaa actgctcaat gcgcgcgcca tcccctgctg acgtatcatt
    65941 gcggaattcg agcaccaaca ggtccgggtt ttccgtgctg tatacggttt tcgctttacc
    66001 acgatacaac tcagcttgct tttgcatctt tatcactcct gggtgtgaat taacgtttta
    66061 aaatcttttg ctgtctggtg tgccggatgt tttgtcggat gcggcgtgaa cgccttatcc
    66121 gaactacgtc cgacaaacaa atttcgtgcg aattaccgct aaaatcgcgt tttcctgccg
    66181 acgcacacgt ttgcgtatca tatcagaaaa aagggccgga tgattccagc cctgtatttt
    66241 tacttgctaa acgcagcctg gaagacagct accagcgcgt cgttctgact ctgagtcaga
    66301 gtatgacctt tcggatcgat gaactgtagg ctgctgcggt tatctaaatc gccaacctgc
    66361 agtttatagt caccggatgc caggcctgga tcgctcgcgc ccagttcctg ccagtcgctg
    66421 tcagacagcg gcttataagt tacggccatg ttgccctgcg aacgggtgct gtcggtcact
    66481 ttcatgccca ctttttccag cgccgctggc agacgttgcc aaaccacatt gaacggcccg
    66541 cgtacgacca gcattggtaa accggtgtca tcagctgcac tttgtacgtc catagtggtg
    66601 gaggcacgat tttgcgcagc gttcgcggcg tcagtggcag atttatccag accggcggaa
    66661 ataacgttca tcatctccgt gctgtaacgc tgcatggaag ccgcgtctgc aaccggtttg
    66721 cccgcctgtt ccaggttcag cagtttaacc gtaaccgcct gctgataacc ctgcggctta
    66781 acagagattt gataacgacc acgatactgc tcgtcttcgt ccagacggtt ccattgtacc
    66841 caatcggtgg tcagtgtctg accagcatca tcacgttggg tgatggtgta gtttttcgcc
    66901 tgcagcacgc taaccacctg cggccacaga gtattgccac gaccattttc caccagcaat
    66961 gaagcggtat cgcccgtgaa ctgggtacgc gcgccagaaa ccagtgccaa cggctgggct
    67021 ggtggacgaa tgtccagcgc cttaccgaca gcaccactac cgttggtcac cgggattgca
    67081 taatcaccgg aggtcaccgg caaaatcatt ccagccgggg catgaagctc cgcaagcggt
    67141 gccgcttcca ggtaggcttc atcaccactg acctgacgct tatagcgtga gtcagaacta
    67201 caggcagcga gtaataaaac aagcgaaaca cccgcaacct ttgccaggcg cgacttttga
    67261 acagagtaag ccatcaaatc tccctaaact ttacagcaaa ccggcatgct taagcgccgc
    67321 tctgaccgtc tcacgaccac tgtcggtgat tggtgtcatt ggcaggcgca gcgtatcggt
    67381 cgccacaaga cccagttcct tacatgccca tttcaccggg attggattgg gttcgacaaa
    67441 tagtttgttg tgtaatggca tcagacgctg attaataacg cgtgcctcgg caaaatgccc
    67501 ttctgctgcc agtttgcaca tctgggccat atcacgcgct gcgacgttag ccgtaacgga
    67561 aataactcca tgaccgccca attgcatgaa gtccagcgcg ctcgcatcat cgccgctcag
    67621 cagaacaaaa tcatctgaaa ccagctcttt gatctggttt acacgcgtta agttccctgt
    67681 tgcctctttg attccgataa tattttttac tttcgccaga cggcccaccg tttccgggag
    67741 cagatcgcag ccagtacggg acggcacatt atacagaatt tgcggcaggt cagtatgctc
    67801 agcgatggct ttgaaatgct gatacaaacc ttcttgcgac ggacgattgt agtaaggggt
    67861 taccgtcagg cagccgacga taccactgtc attgaagcgc tgcgtcaggc taatggcttc
    67921 cgcagtagcg ttagcgccgg tcccggcaat taccggaatg cgcccatcag ccagatccag
    67981 cgtcatcatc accacatcag catgttcgtc atgatttaag gtagcggact cgccagtggt
    68041 gccaacagaa acgatcgccg aagtaccgct ggcgacatga taatcaatca gttttttcaa
    68101 gctagcccga cagacattac ctttttcatc catcggagta acaatcgcga caatacttcc
    68161 cgtgaacatg ggccatcctc tgtgcaaaca agtgtctcaa tggtacgttt ggtatggcat
    68221 taaaagcaag cagacagaac cgttctgatt gttgtatgca tgattttttt atgctttcct
    68281 taagaacaac tcacccctta aaggaataac cagtttgaca ctgtcatcgc aacattatct
    68341 ggtgatcact gcgttgggtg ccgatcgccc tggaattgtg aacaccatca cccgtcatgt
    68401 cagtagttgc ggctgtaata ttgaagacag tcgcctggcg atgctgggag aagagttcac
    68461 gtttatcatg ctgctttccg gttcatggaa tgccatcact ctgattgaat caacgttacc
    68521 gttgaaaggt gccgaactgg atcttttaat cgtgatgaag cgcacgacgg cgcgtccgcg
    68581 tccgccaatg ccagcatctg tctgggttca ggtcgatgtt gcagactccc cgcatttaat
    68641 tgaacgcttc acagcacttt tcgacgcgca tcatatgaac attgcggagc tggtgtcgcg
    68701 cacacaacct gctgaaaatg aacgggctgc gcagttgcat attcagataa ccgcccacag
    68761 ccccgcatct gcggacgcag caaatattga gcaagcgttc aaagccctat gtacagaact
    68821 caatgcacaa ggcagtatta acgtcgtcaa ttattcccaa catgatgaac aggatggagt
    68881 taagtaatga atccactgaa agccggtgat atcgcaccga aatttagctt gccggatcaa
    68941 gacggagaac aagttaattt gaccgacttc cagggacagc gtgttctggt ttatttctac
    69001 ccgaaagcca tgacccccgg ctgtaccgta caggcctgcg gcttacgcga taacatggat
    69061 gagttgaaaa aagcgggcgt tgatgtgctg ggtatcagca ccgataaacc cgaaaaactc
    69121 tcccgttttg cggaaaaaga gctgcttaac tttacgctcc tgtctgatga ggaccaccag
    69181 gtgtgcgaac aattcggcgt ctggggtgaa aagtccttca tgggcaaaac ctacgatggt
    69241 attcatcgca tcagcttcct gattgacgct gatggcaaaa tcgaacatgt ctttgacgat
    69301 ttcaaaacca gcaatcacca cgacgttgtg ctgaactggc tgaaagaaca cgcctgatta
    69361 ctttgctcca ttccgtgctg gctgcgcttg cggccagcat acctcacttc tcgtgatcaa
    69421 gatcacattc tcgctttccc ctgcgacacg ggtgtcgaat ccattttttg ctgaacgtta
    69481 atgaccatca tttttgtacc gttcagaatc cagttaatac ataacttatt gaatagattg
    69541 agttaatcag aatggcatcc tttatacaat atgaaatgca atgtttcata tcattttcaa
    69601 ggagccgaca tgaaccgctt tgtggtggcc gaaccactgt ggtgtacagg atgtaatacc
    69661 tgtctcgctg cctgttcgga cgtgcataaa acgcaaggtt tacagcaaca cccgcgcctg
    69721 gccctggcga agacgtcaac aatcactgcc cctgtcgtgt gtcatcactg tgaggaagcc
    69781 ccttgcctgc aggtctgccc ggtcaatgcc atctctcaga gggatgatgc gatccaactc
    69841 aacgaaagcc tctgtattgg ctgcaagctt tgcgccgtgg tctgcccatt tggcgcaatc
    69901 agcgcttcag gaagccgtcc ggtgaatgcc catgcgcaat atgtttttca ggctgaaggc
    69961 tcactcaaag acggcgaaga aaacgtgccg ccccaacatg cgttgctgcg ctgggaaccg
    70021 ggtgtccaga ccgtcgcggt gaaatgcgac ctgtgtgatt tcttgccaga aggtccggcc
    70081 tgcgttcgcg cttgcccgaa tcaggcgtta cggctgatca ccgacgatag cctgcaacgt
    70141 cagatgaaag aaaaacagcg ccttgccgca agctggtttg ccaatggcgg ggagtctccc
    70201 ctttccctca ctcaggagca acactaatgg atgccctgca attattaacc tggtcgctga
    70261 tcctctatct gtttgccagt ctggcttcgc tgtttttact cggtctggac agactggcta
    70321 ttaagctttc cggcatcaca tcgctggtgg gcggcgtgat tggcatcatc agcggaatta
    70381 cgcaattaca tgctggtgta actttagtcg cccattttgc cacccctttt gactttgccg
    70441 atttaaccct gcgaatggat agcctctcgg catttatggt gctagttatc tccttgctgg
    70501 tggtggtttg ttcgctctat tcattgactt atatgcgcga atacgagggc aaaggcgcgg
    70561 cggcgatggg cttctttatg aatcttttca tcgcatcgat ggttgccctg ctggtgatgg
    70621 acaacgcttt ttggttcatc gtgctgtttg aaatgatgtc gctgtcttcc tggtttctgg
    70681 tcattgccag gcaggataaa acgtcgatca acgctggcat gctctacttt tttatcgccc
    70741 acgccggatc ggtgctgatt atgatcgcct tcttgctgat ggggcgcgaa agcggcagcc
    70801 tcgattttgc cagtttcgca cgctttcact ttctccgggg ctggcgtcgg cggtgtttct
    70861 gctggccttt ttcggttttg gcgcgaaagc cgggatgatg ccgttgcaca gctggttgcc
    70921 gcgcgctcac cctgccgcac catcgcacgc ttcggcgttg atgtctggcg taatggtcaa
    70981 aatcggtatt ttcggcatcc tgaaagtagc gatggatctg ctggcgcaaa cgggtttgcc
    71041 tctgtggtgg ggcattctgg tgatggcgat cggcgcaatc tccgcgctcc tgggcgtgct
    71101 atatgcgctg gcggaacagg atatcaaacg gctgctggcc tggagtaccg tcgaaaacgt
    71161 cggcattatt ttgctggcag tcggtgtggc gatggacggt ctgtcactgc acgacccgct
    71221 gctcaccgtg gttggactgc tcggcgcact gtttcatctg ctcaaccatg cgctgttcaa
    71281 agggctgcta tttctcggcg cggacgcgat tatttcgcgt ttgcataccc acgacatgga
    71341 aaaaatgggg gcactggcga aacggatgcc gtggacagcc gcagcatgcc tgattggttg
    71401 cctcgcgata tcagccattc ctccgctgaa tggttttatc agcgaatggt acacctggca
    71461 gtcgctgttc tcactaagtc gtgtggaagc cgtagcgcta caacttgcgg gtcctattgc
    71521 tatggtaatg ctggcagtca ctggtgggct ggcagtaatg tgcttcgtca aaatgtacgg
    71581 tattactttc tgtggtgcgc cgcgcagtac acacgctgaa gaggcacagg aagtgccaaa
    71641 tacgatgatc gtcgccatgc tactgctcgc ggcactctgc gtattaattg cgcttagtgc
    71701 cagttggctg gcaccgaaga taatgcatat tgcccatgcg tttaccaata cccctcccgt
    71761 cactgtcgcc agcggaatag cacttgtacc cggcacgttt catacacggg tcactccttc
    71821 attactgttg ctgttactac tggcgatgcc tttgctgcca ggcctttact ggctgtggtg
    71881 tcgttcgcgc cgcgcagcgt ttcgtcgcac aggagatgcc tgggcatgcg gctacggctg
    71941 ggaaaacgcg atggctccgt caggcaatgg cgtgatgcag ccgctgcgtg tggtcttctc
    72001 tgcgttattt cgtctacgac aacagctcga ccctacgctg aggctaaata aaggtcttgc
    72061 gcacgtcacc gccagggctc agagcacaga acccttctgg gatgagcggg tgatccgccc
    72121 catcgtgagc gccacccaac ggctggtcaa agaaatacag catctgcaaa gcggcgactt
    72181 tcgtctctat tgcctgtatg tggtcgccgc actggttgtg ctgctaatcg ctattgccgt
    72241 ctaaggaaat caccatgaga caaactcttt gcgacggata tctggtcatt tttgcgttag
    72301 cacaggccgt gattctgctg atgctaaccc cactttttac gggtatttcc cggcagatac
    72361 gcgcgcgtat gcactcccgc cgcgggccgg ggatctggca ggattatcgc gatatccaca
    72421 aactgtttaa acgccaggaa gttgcgccga catcttcagg tctgatgttc cgcctgatgc
    72481 cgtgggtatt aatcagcagc atgctggtgc tggcgatggc cttaccacta tttattaccg
    72541 tttccccttt tgcgggcggc ggcgatctga tcacccttat ctatcttctt gccctgtttc
    72601 gttttttctt tgctctttcc gggctggata ccggaagtcc gtttgcggga gtcggtgcca
    72661 gtcgcgagtt gacgctcggc attctggtcg aaccaatgct tattctctca ctgctggtat
    72721 tggcgctgat agcaggttcc acgcatatcg agatgatcag caatacgctg gctatgggct
    72781 ggaactcgcc gctaaccacc gtactggcgt tactggcctg tggttttgcc tgcttcattg
    72841 agatgggaaa aattcccttt gatgttgctg aagcagaaca ggaattacag gaaggcccgc
    72901 tgaccgaata ttccggtgcc gggctggcgc tagcgaaatg ggggctgggg ctgaaacagg
    72961 tcgtgatggc agcactgttt gtggccctgt ttctgccctt tgggcgcgcg caagaacttt
    73021 ctctcgcctg cctgctgact tcacttgtcg ttacgctgct caaggttttg ctgatttttg
    73081 tactggcctc aatcgcagaa aacacgctgg cacgcgggcg ttttttactc attcaccatg
    73141 tgacctggct tggcttcagc cttgctgcgc ttgcatgggt cttttggtta accggtctgt
    73201 aaggagcact gacggaatat ggaaaatctt gctctgacga cgttaatgct gccttttatc
    73261 ggcgcactgg tcgtttcgtt ttcgccacaa cgtcgggccg ccgaatgggg ggttttgttc
    73321 gccgcgctga ccacgctgtg catgttgtcg ctgatctccg cgttttatca ggccgataaa
    73381 gttgccgtca cgttgacgtt ggtcaacgtg ggggatgtgg cattatttgg cctggtcatt
    73441 gatcgcgtga gtacgctgat tctgtttgtg gtggtgtttc tcggtttgct ggtcacgatc
    73501 tactccacgg gttatctgac ggataaaaat cgcgaacacc cgcataacgg cacgaatcgt
    73561 tattacgcat ttttgctggt gtttatcggc gcgatggcgg gactggtact ctcctcgacg
    73621 ctgctcggtc agttgttgtt ttttgaaatt acgggcggct gctcctgggc gttgatcagt
    73681 tattaccaga gcgataaagc gcagcgttca gcactaaaag cgttgcttat cacccatatc
    73741 ggttcgttgg ggttgtatct tgccgccgcc acgctgtttt tgcagaccgg aacgtttgtg
    73801 cttagcgcga tgagcgagtt acatggcgac gcacgttatc tggtttatgg cggcatcctg
    73861 tttgccgcgt gggggaaatc ggcccagcta ccgatgcaag cgtggctacc ggacgcaatg
    73921 gaagcgccaa caccgatcag cgcctatctc cacgccgcat cgatggtgaa agtgggcgtt
    73981 tacatttttg cccgcgccat tatcgacggc ggcaatatcc cgcatgtgat tggcggcgtt
    74041 ggcatggtca tgacactggt caccattctt tatggctttc tgatgtattt gccacagcag
    74101 gatatgaagc ggttgctggc ctggtcgacc atcactcaac ttggctggat gttcttcggc
    74161 ttgtcgctct ccatcttcgg ctcgcggctg gcgctggagg gtagcatcgc ctacatcgtc
    74221 aaccacgcgt tcgctaaaag cctgtttttc cttgtagcag gtgcgctgag ttacagctgc
    74281 ggcacgcgct tgttgccgcg tctgcgtggc gtattgcaca ccctgccgtt gccaggcgtg
    74341 ggtttctgcg tggcagcgct ggcaattacc ggtgtgccgc cgttcaacgg cttcttcagt
    74401 aaattcccgc tgtttgccgc cggttttgcg ttgtcagtgg agtactggat cctgctgccc
    74461 gccatgattc tgctgatgat tgaatcggtc gccagtttcg cctggtttat tcgctggttt
    74521 ggtcgcgtcg tgccgggcaa accgagcgag gccgtcgccg atgccgcacc gctgccaggg
    74581 tcaatgcgcc tggtgttgat tgtactgatt gtgatgtcgc tgatttccag cgtaatcgcc
    74641 gcgacctggt tgcagtaagg agatgatgaa tgaccggttc tatgatcgta aataatctgg
    74701 cgggactgat gatgctgaca tcgctgtttg tgattagcgt caaaagctat cgcctgtcat
    74761 gcggatttta cgcctgccag tcgctggtgc tggtgtctat tttcgccact ctctcgtgcc
    74821 tgttcgccgc agagcaactg ctgatctggt ccgccagcgc ctttatcacc aaagtgctgc
    74881 tggtaccgtt aatcatgact tacgctgcac gaaatattcc ccagaacatc ccggaaaaag
    74941 cgttattcgg tccggcaatg atggcactgc tcgcggcgtt aattgtcctg ctttgcgcat
    75001 ttgtcgttca gcccgtgaag ctaccgatgg ctaccgggct gaaaccggcg ctggcggtag
    75061 cgttaggtca ttttctgctt ggcctgctgt gcattgtcag ccaacgcaat atcctgcggc
    75121 aaatttttgg ttactgcctg atggaaaacg gctcccatct ggtgctggcg cttcttgcct
    75181 ggcgagcacc ggaactggtg gaaataggta tcgctaccga cgccatcttc gccgtcattg
    75241 tgatggtgtt actggcaaga aaaatatggc gtacccacgg cacgctggac gtgaacaact
    75301 tgaccgcgct gaagggataa tgagatgagt tattctgtga tgttcgcttt actcctgctc
    75361 acgccgctgc ttttttcgct gctctgtttt gcctgccgga aacgggggct ctctgcgact
    75421 tgtacggtga ctgtattaca tagcttaggg atcacactgc tgctgattct agcactctgg
    75481 gtggtccaaa ctgccgctga tgcaggagaa atattcgctg cgggactgtg gcttcatatt
    75541 gatggtctgg gcggtttgtt cctcgccatt cttggtgtga ttggctttct caccggtgtt
    75601 tactcgattg gctacatgcg tcatgaagtg gcacacggcg agctttcacc cgttacactg
    75661 tgcgattact acggtttctt ccatctgttt ttgttcacca tgctgctggt tgttaccagc
    75721 aataacctga ttgtgatgtg ggcggcgatc gaagccacca ccttaagctc ggcgtttctg
    75781 gtaggcattt acggtcagcg ttcatcgctg gaatcagcat ggaagtacat cattatttgt
    75841 actgttggtg tcgcttttgg tctgttcggt accgtgctgg tatacgccaa cgccgccagc
    75901 gttatgccgc aggcagaaat ggcgatattc tggagcgagg ttcttaagca atcgtccttg
    75961 cttgacccaa cattaatgct gttggccttt gtgtttgtgc taattggctt tggtaccaaa
    76021 accgggctat ttcccatgca cgcctggctg ccggatgctc acagtgaagc gccgagtccg
    76081 gtcagcgccc tgctctccgc cgtattgctg aactgagcgc tgttggtgct gattcgctat
    76141 tacatcatta tttgccaggc catcggcagc gatttcccca accggttgtt gctcatcttc
    76201 ggcatgttgt cggttgccgt ggcggcattt ttcattctgg tacagcggga cattaagcgt
    76261 ctgctggcgt actccagcgt ggagaacatg gggctggtcg cggtggcgct aggcattggc
    76321 gggccgctgg gaatttttgc cgcgctgctg cacaccttaa accacagtct ggcaaaaacg
    76381 ctgctgttct gcggttccgg caatgtactg ctcaagtacg gcacgcgcga tctcaacgtc
    76441 gtctgtggga tgctcaaaat catgccattt accgccgtgc tgtttggcgg cggtgcgctg
    76501 gcgctggcgc tggcagggat gccgcccttc aacatttttc ttagcgaatt tatgaccgtt
    76561 accgcaggac tggcacgtaa tcacctgctg attatcgtcc tgctgttatt gctgttaacg
    76621 ctggtgctgg cgggcctggt acggatggct gcgcgggtgt taatggcgaa accgccgcag
    76681 gccgttaacc ggggtgatct cggctggttg accacctcgc caatggtgat tctgctggtc
    76741 atgatgctgg cgatgggaac gcatattcca caacctgtca tcaggatcct ggcgggcgct
    76801 tccactatag tcctctcagg gacgcacgac ctgcctgcac aacgtagcac ctggcatgat
    76861 tttttgcctt caggcaccgc atctgtttcg gagaaacaca gtgaacgtta attcatcgtc
    76921 aaatcgtggc gaagcgattc tcgccgccct gaaaacgcag ttccccggcg cggtgctgga
    76981 tgaagagcga caaacgcctg aacaggtcac cattacggtg aaaatcaatc tgctgcctga
    77041 cgttgtacat tatctttatt atcaacatga tggctggctt ccagtcctgt ttggcaacga
    77101 cgagcggaca cttaacggtc attacgcggt ttattatgcc ctttcaatgg aaggggccga
    77161 aaaatgctgg atcgtggtga aggcactggt cgatgccgac agtcgggagt ttccgtcagt
    77221 cacaccgcgc gtccctgccg cggtctgggg cgagcgagaa attcgcgata tgtacgggct
    77281 gattccggtt ggcctgccgg atcagcgtcg cctggtgttg cccgatgact ggccggaaga
    77341 tatgcatccg ctgcgcaaag atgcgatgga ttatcgactg cgccctgaac cgacgacaga
    77401 ttccgaaacg tatgcgttta tcaatgaggg caacagcgat gcgcgggtga tccctgtcgg
    77461 cccgctgcat atcacctcct atgaaccggg tcacttccgc ttgtttgtgg atggcgagca
    77521 aattgtcgat gctgattacc gcctgtttta tgtccatcgc ggcatggaga aactggcaga
    77581 aacgcggatg ggctacaacg aagtgacctt cttatcggac cgcgtgtgtg ggatttgcgg
    77641 ttttgcccac agtgtggcct ataccaactc ggttgaaaat gcactgggga ttgaggtgcc
    77701 gcaacgagca catactattc gctcgattct gctggaagtc gagcggctac acagtcattt
    77761 gctcaacctt ggcctctcct gccattttgt tggttttgat accggcttta tgcaattttt
    77821 ccgcgtgcgg gaaaagtcga tgacgatggc ggaattgctg accgggtcgc gtaaaaccta
    77881 cggtctgaat ctgattggtg gtgttcgccg cgatattctc aaagagcaac gtctgcaaac
    77941 gctgaaactg gtgcgcgaga tgcgcgccga cgtgtcggag ctggtagaga tgctgcttgc
    78001 tacgccgaat atggaacaac gcactcaggg cattggcatt ctcgaccgac aaatcgcccg
    78061 tgattatagc cctgtagggc cgctgatccg cgcagtggtt ttgcccgtga tttgcgcttt
    78121 gatcacccct acgccgacta cggcaatatt ccaaaaacac tgtttacctt taccggcggc
    78181 gatgttttct cccgcgtgat ggtccgtgtc aaagagacgt ttgattcgct ggcaatgctg
    78241 gaatttgccc tcgacaacat gccggatacc ccactgctga ccgaaggctt tagctataaa
    78301 cctcacgcat tcgcgctggg ctttgttgaa gcgccacgcg gtgaagacgt gcactggagc
    78361 atgctcggtg ataaccaaaa attgttccgc tggcgctgtc gtgccgccac ctacgccaac
    78421 tggccggtgt tgcgttacat gctgcgcggc aataccgttt ctgacgcacc gctgattatc
    78481 ggtagccttg atccctgcta ctcctgtacc gaccgtgtga cgctggtaga tgtgcgcaag
    78541 cgccagtcaa aaaccgtgct gtataaagag atcgaacgct acggcattga tcgtaaccgt
    78601 tcgccgctga agtaaggaca gaagatgctg aagttactga aaactattat gcgcgccgga
    78661 accgcgacgg tgaaatatcc cttcgcgcca ctggaggtca gcccaggctt tcgcgggaaa
    78721 ccggacctga tgcccagcca atgtattgcc tgcggtgcct gcgcctgtgc ttgtccggca
    78781 aatgcgctga ctatccagac cgacgaccag caaaattcgc gcacctggca gctctatctg
    78841 gggcgttgta tttactgcgg acgttgtgaa gaagtgtgcc cgaccagagc catccagctt
    78901 accaataact ttgaactgac cgtcacccat aaagccgatc tctataccca cgcgacgttc
    78961 catctacaac gttgcagccg ttgcgaacgc ccgtttgccc cgcaaaaaac cgtcgcactg
    79021 gctgctgaat tgttggcaca gcaacaaaat gctccacaaa accgcgaaat gttatgggcg
    79081 caagcgagcg tctgcccgga gtgcaaacaa cgcgcaacgc tgatcaacga cgatacagat
    79141 gtaccgctgg tggctaagga gcagctatga gtccagtgct tacgcaatat gtcagccagc
    79201 ccatcacgct ggacgagcaa acgcaaaaga tgaagcggca tttgctacag gatatccgtc
    79261 gctcggctta cgtttatcgc atcgattgcg gcggctgcaa cgcctgtgaa atcgaaattt
    79321 ttgctgccat cacccccgta ttcgacgcag aacgttttgg cattaaggtt gtttcatcac
    79381 cgcgtcacgc cgatattttg ttatttactg gcgcagtcac ccgggcgatg cgtatgcctg
    79441 cacttcgggc gtatgagtct gcccccgatc ataaaatctg tgtttcctac ggcgcgtgcg
    79501 gtgtcggcgg cggtattttc cacgatctct acagcgtctg gggcggcagc gacaccattg
    79561 tccccattga tgtttggatc cccggctgcc cgccaacacc ggccgccacc attcacggtt
    79621 tcgccgtggc gctcggtttg ctgcaacaga agattcacgc ggtggattat cgcgatccca
    79681 ccggggtgac tatgcaaccg ttgtggccgc agatcccgcc atcacagcgt atcgccattg
    79741 agcgagaagc gcggcggctg gcgggctatc gtcaggggcg agaaatttgc gatcggctcc
    79801 tgcgccattt aagcgacgat cctacaggaa atcgggttaa cacctggttg cgcgatgccg
    79861 acgatccacg tctcaatagt atcgttcagc aactctttcg cgtactccgg gggttacatg
    79921 actgaagagt gcggggaaat tgttttctgg acgctgcgaa aaaagtttgt cgccagtagc
    79981 gacgagatgc cggaacacag ctctcaggta atgtattact cgctggctat cggtcatcac
    80041 gttggcgtga ttgattgtct gaatgtcgcc ttccgctgcc cactgacgga atacgaagat
    80101 tggcttgcac tggtcgaaga ggagcaagcc cgacgtaaga tgctgggggt gatgactttt
    80161 ggtgagattg ttattgacgc cagccacacc gccctgttga cccgggcatt cgcgccactg
    80221 gcagatgacg cgacgtctgt gtggcaagcg cgtagcattc aattcattca tctgttggat
    80281 gaaattgtgc tggaaccggc tatctatctg atggccagaa aaattgcgtg agaaggattt
    80341 ctcattaata aggactgttg atggctatgt cagacgaagc gatgtttgcc ccgccgcaag
    80401 gaataacaat tgaagcggta aacggaatgc tcgcggagcg gttagcgcag aaacacggca
    80461 aggcgtcttt attacgcgcc ttcatcccgc tgccgccgcc gttcagcccg gtacaactta
    80521 ttgaactgca tgttctcaaa agcaacttct attaccgcta ccatgatgat ggcagcgatg
    80581 tgacggcaac aacagagtat cagggcgaga tggtcgatta ttcgcgttac gccgtccttc
    80641 tcggcagtag tggaatggcg gagctacgct ttattcgtac ccacggcagc cgttttactc
    80701 cccaggattg cacactgttt aactggctgg cacgcattat caccccggtt ctgcaatcat
    80761 ggctcaatga tgaagcacag caggtggcgc tgcgtttgct ggagaaagat cgcgatcatc
    80821 atcgggtact ggtggatatc actaatgcag tgctgtcaca tcttgatctc gacgatctga
    80881 tcgctgacgt cgctcgtgag atccatcatt ttttcggtct ggcttcagtc agtatggtac
    80941 tgggcgatca tcgaaagaac gagaagttca gcctgtggtg cagcgatctt tctgcctcac
    81001 attgtgcgtg tctgccacac aatatgcctg gcgacagtgt attgctgaca caaacgctac
    81061 aaacccgaca accgaccttg acgcaccgtg cagatgatct gtttctctgg caacgcgacc
    81121 cgttattact cttacttgca tctaacggct gcgaatctgc gctccttata ccgcttacct
    81181 ttggcaacca tacaccgggt gcattgttgc tggcgcatac ctcttccact ctctttagtg
    81241 aggaaaactg ccagctacta caacacatag ccgatcgcat cgctattgcc gttggcaatg
    81301 ccgatgcctg ccgtagaatg accgatttgc aggaaagttt gcagcaagaa aaccaccagc
    81361 ttagcgagca gctcctttcg aatctgggcg tcggtgacat tatctatcaa agccaggcaa
    81421 tggaagacct gctccagcag gtagatattg tgtcgaagag cgacagtacg gtgttgattt
    81481 gcggtgaaac cggaaccggc aaagaggtga tcgccagagc gatccatcaa cttagcccgc
    81541 gacacgacaa gccgctggtc aaaatcaact gcgctgccat ccccgccagt cttctggaaa
    81601 gtgagttatt cggtcatgac aaaggggcgt ttactggtgc gattaatacc catcgtggtc
    81661 gttttgaaat tgccgatggc ggcacgttgt ttctcgatga aattggcgat ctgccgttag
    81721 aacttcagcc taaactgctg cgcgtattgc aggaacggga gattgagcgt ctcggcggga
    81781 gtagaacgat cccggtaaat gtcagagtca ttgccgccac caaccgtgat ttgtggcaaa
    81841 tggttgaaga tcgccagttt cgcagcgatc tcttttatcg cctgaatgtc ttcccactgg
    81901 aattgcctcc gctgcgcgac cgtccggaag atatccctct tttagcaaag catttcacgc
    81961 aaaaaatggc gcgccatatg aatcgttcaa ttgacgccat cccgaccgag gcgctacgcc
    82021 agttgatgtc gtgggactgg ccgggcaacg tgcgcgagct ggaaaacgtg attgagcgag
    82081 cggtactgct gactcgcggt aacagtctga atttacatct taatgtccga caaagccgtt
    82141 tactgccgac gctaaatgaa gattcagcgc ttcgcagttc aatggcgtag ttgctgcacc
    82201 cgacgacgcc agagaatgac gaagaagaac gtcagcgcat tgttcaggta ttgcgagaaa
    82261 ccaatggcat tgttgccggg ccccgtggcg cggcgacacg attagggatg aagcgcacca
    82321 cgctgctgtc acgaatgcag cgtctgggaa tctcggttcg cgaggtgttg taatctgctt
    82381 ttgcaggagt atgcatgaga aacaaactct ctttcgactt gcagttgagc gccagaaaag
    82441 cggcaatcgc tgaacggatt gccgcccata aaattgcccg cagtaaagtg tcggtctttt
    82501 taatggcgat gtccgctggc gtgtttatgg cgatcggatt tactttttac ctttccgtta
    82561 tcgccgatgc cccgtcttca taggcattaa cccatctggt gggcggcctt tgctttacac
    82621 ttggctttat tttgctggcg gtttgcggca ccagcctgtt cacctcgtcg gtaatgacgg
    82681 taatggcaaa aagtcggggc gttattagtt ggcgaacttg gctgattaac gcacttctgg
    82741 tggcctgcgg taatctggca ggtattgcct gtttcagttt gttaatctgg ttttccgggc
    82801 tggtgatgag tgaaaacgcg atgtggggag tcgcgatttt acactgcgcc gagggcaaaa
    82861 tgcatcatac atttactgaa tctgtcagcc tcggcattat gtgcaatctg atggtttgcc
    82921 tggcgctgtg gatgagttat tgcggacgtt cgttatgcga caaaatcgtc gccatgattt
    82981 tgtccatcac cctgtttgtc gccagtggct ttgagcactg tatcgccaat ttgtttgtga
    83041 ttccgttcgc cattgccatt cgccatttcg cccctacctc cttctggcaa ctggcgcaca
    83101 gtagcgcaga caattttccg gcactgacgg tcagccattt tattaccgcc aatctgctcc
    83161 cggtgatgct gggtaatatt atcggcggtg cagtgctggt gagtatttgt tatcgggcta
    83221 tttatttacg tcaggaatcc tgaaaaatca gcccggcgaa acagttcgtc gggctgaaga
    83281 ttattcttgc gcgatttgcc catcgggcca ggcgtggatc acggctttga tcagcgtcgc
    83341 caatggaatg gcgaagaata cgccccagaa tccccacaaa ccaccgaaga tcaccaccga
    83401 taaaataatc accagcggat gcaggttaac cgcttcggag aacaacaccg gtactagcag
    83461 gttgccgtcc agcgcctgaa taatcagata caccgcgaag cagctccaga attcagtacc
    83521 tgcgccaaac tggaatagcg ccacgccaac caccggaatg gtcaccacaa atgcgccaat
    83581 gtacggaata agaaccgaga agccgaccag cactgccagc agcagcgaat agttcagccc
    83641 gaagagcaag aaccccagcc aggtggcgat ccccaccacg atcatctcca gcactttgcc
    83701 gcggatatag ttggtgattt gttgattcat ctccttccac acctgtcctg ccagcccacg
    83761 gttacgcggc agcacccggc gaacggcgtt cagcatctgc tctttgtctt tcagcaggaa
    83821 gaagaccatc aatggcacca gcaccagata gacggctatg gtcagcaaac cgaccagcga
    83881 ggcgagggaa attttcacca ccgaatcgcc catggtcagc atccgactgc gcatattttc
    83941 ggccattgca tcaataatgc ccgcgtccat taacaccgga tagcggcgcg gcaacgtggc
    84001 ggcaaagtca gaaagcttat tgagcatccc cggcatatcg cggattaagt agatgccctg
    84061 ttgccaggcg ataggcagta ccacgaacgc catcagtagc aatataccga caaaaaccac
    84121 caatacaatc gacgtcgccc agcggcggga gcagccaata gattgcaggc gcacggttgg
    84181 ccattccagc aaataagcca gcacaatagc caccagcaac ggagcaagca ggccactaaa
    84241 gaaaaagata atgccaaatc cggcaactaa aataaccagc aaggcaatcg cttccgggtc
    84301 gctaaaacgg cggcgatacc attgcatcaa catttcgagc ataaagacct tccctgaacc
    84361 tcaagagcgg gattgcgatc cgcaattgta tcgaaatgtc acaaaaaaga cttcgctttt
    84421 tatgacggat tcaggaaact gaaaagtcat ttgagtgggc taatcttcgc cgttacactc
    84481 aaaggcggcg aggtgggaac gatatttcac agtatcggtc aaatgactat cttcagaaat
    84541 acaggataga ggttatgttc aggcagttga aaaaaaacct ggttgcaacc ctcattgctg
    84601 ctatgaccat tggtcaggta gccccggcgt ttgccgacag cgcagacacc ttgccggata
    84661 tgggaacctc cgcaggaagc acgctttcca ttggtcagga aatgcagatg ggcgactatt
    84721 atgtccgcca gctacgcggc agcgcgccgt taattaatga cccgctgtta acgcaatata
    84781 ttaattcgct ggggatgcgt ctggtttcgc atgccaattc ggttaagaca ccgtttcatt
    84841 tttttctgat caacaacgac gaaattaacg cctttgcttt ctttggcggc aacgtggtgc
    84901 tgcactctgc cctgttccgt tattccgata acgaaagtca actggcttca gttatggcgc
    84961 acgaaatctc ccacgtcacc caacgtcacc tggcgcgagc gatggaagat caacaacgca
    85021 gcgcgccgct gacctgggtc ggcgcgttag gttctatttt actggcgatg gccagtccgc
    85081 aggcggggat ggcggcgctg accggtacac tggcgggaac gcgccagggg atgatcagtt
    85141 tcacccagca aaatgaacag gaagcggacc gcattggtat tcaggtgctg caacgctcgg
    85201 gattcgatcc acaggcgatg ccgaccttcc tggaaaaatt gctcgatcag gcgcgttact
    85261 cctcgcgccc gccggaaatt ttattgactc acccgttgcc ggaaagtcgt ctggcagatg
    85321 cccgcaaccg tgctaatcag atgcgcccga tggtggtgca gtcgtcggaa gatttctatc
    85381 tggcgaaagc gcgcacactg gggatgtata attccggacg taaccagctc accagtgatt
    85441 tgctggatga atgggcgaaa ggaaacgttc gtcagcaacg agcggcgcaa tatggtcgtg
    85501 ctttacaggc gatggaagcc aataaatacg acgaggcgcg taaaacgctg caaccgttac
    85561 tggcggcaga acctggcaac gcatggtatc tcgatctggc taccgatatc gatcttgggc
    85621 aaaacaaagc caatgaggca atcaatcgcc tgaaaaatgc ccgcgattta cgcaccaatc
    85681 cggtgttgca gctcaacctg gcgaacgctt atctgcaagg cggtcaacca caagaagcgg
    85741 ccaatattct gaatcgctac acctttaata ataaagatga cagcaacggc tgggatttgc
    85801 tggcacaggc ggaagccgcg ctaaataacc gcgatcagga gctggctgcg cgagcagaag
    85861 gttatgcgct cgccggacga ctcgatcagg ccatttcgct gttgagtagc gccagttcgc
    85921 aggtgaaatt aggcagcctg caacaagcgc gttacgatgc gcgcatcgac cagttgcgcc
    85981 agctgcagaa acgctttaag ccttatacca agatgtaatt cgcattaaag gaagaacaat
    86041 gaccaaacag gtaaaaatct accataaccc acgctgttca aagagccggg aaacgctgaa
    86101 cctgctgaaa gaaaacggcg tggagccaga agtggtgctc taccttgaga cacccgccga
    86161 tgcggcaacg ctgcgcgatt tgctgaaaat gctggggatg aatagcgccc gtgaattgat
    86221 gcgccagaaa gaggatcttt ataaagaact aaacctggct gacagttcgc ttagcgaaga
    86281 ggcgctgatt caggcaatgg tggataatcc gaagctgatg gagcgcccga ttatggtcgc
    86341 gaatggcaaa gcgcggattg gtcgtccacc ggagcaggta ctggaaatcg tgggttaaga
    86401 tgtgctccat cgctagttga agcacattgc cggatgcgac gcttgccacg tcttatccgg
    86461 cctacgaata ctatggatta acttcgcgta ggtcaggcgt tcgcgccgca tccgacaata
    86521 aacaccttat ctacaacttc agaatttctt tcacaaacgg aatggtcagc ttacgttgcg
    86581 cggtaatcga cgcacgatcc aactgatcca acgtcataaa tagcgtgcgc atttctctgt
    86641 cgagccgctt cagcaagaaa cgccccacat cttccggcag ttcaaaacca cgcaaacgcg
    86701 cgcgtaactg tagcgcctgc aacttatctt catcagaaag tggctgcaat ttgtagatct
    86761 gcccccagtc gagacgcgac gcgagatccg gtaatcccag attcaactgc cgcggtggac
    86821 gatcgccggt gatcaacaac cgtgttttgc ccgattccag aattcgattg tagagatcga
    86881 aaatcgccat ctcccacaac tcatcgcctg caatacactc aatgttgtcg atacagacca
    86941 gcgacaaatg ctccataccg tcgagcactt ccggaacaaa ccaggtgcgt ttatccagcg
    87001 ggacatagcc caccgcatcg ccacgctgcg acaattccgc gcaagccgcg tgcagcagat
    87061 ggctgcgccc cgcgccttcg cgtgcccaga gatagatgta accgctatgt tcctgacgca
    87121 gcacgttttg cagcgcggcc agtaaagagg agttatcccc cggccagaaa cttgcaaagg
    87181 tttcgtcgtc aggaagataa agtggcaaag agagctgtgc cggtgtgttc agagatacct
    87241 caaccaggat ttcacaaaat cgcgagaagt ttaccacaga atcccatgat gtttgaaccg
    87301 ggcagcaaca ctgcccggtc ggtacattat ttgtctgtta tatccgcgtc ttctgcgtcc
    87361 agcaccactt cttcagggcg cagcacactg atcagtttga aaatcaaact taagccgata
    87421 ccgacgatgg tcgccagcgc catacctttt aactccgccg cgccgatgtt taccttcgcg
    87481 ccactgacgc cgatgatcaa aatcacggaa gtcaggatca ggttctgtgc tttgttgtaa
    87541 tccactttcg attcgatcaa aacacgaata ccggaagcac caatgacacc ataaagcagc
    87601 agcgaaacgc cgcccatcac cggcaatggg atcatctgga tagcggcagc cagtttaccg
    87661 acgcaggaaa gcaggatagc gaaaatcgcc gccccgccga taacccaggt actgtaaaca
    87721 cgggtgatcg ccatcacgcc aatgttttct ccgtaagtgg tatttggcgt agagccaaag
    87781 aagccggaga tcacggtcga caggccgtta gcaaacatcg aacggtgcag acctggatcg
    87841 cgcagcagat cttttttgac gatattagcc gttactacca ggtgccctac gtgttcggca
    87901 ataaccacta acgccgctgg cagaatagtc agaatggcaa accactcgaa gcgcggcgta
    87961 tagagggttg gcagtgcaaa ccagtgagca ttaataatcg gcgtggtatc gacaattccc
    88021 attgcgaaag agagcgcgta ccccaccagc acgccaatta aaatcgggat aattgccagg
    88081 aaaccacgaa acagcacgga acctaaaacc gtgaccgcca gggtggtaat agagatgatg
    88141 atggttttgg agtttggcgt ttgcccttca gccgggagta aacccgccat accggcagct
    88201 acgcccgcca gctccagacc gatgacggca acgattgcgc ccattgccgc aggtggaaac
    88261 agcacgtcca accagccggt ccccgctttc ttcacgataa aagaaaccag gcagaacagc
    88321 acgccgcaca taataaagcc gcccagcgcg acttcatacc ctaacggcaa cagtaacaat
    88381 accggtgaaa taaaggcaaa gctggaacca agataagccg gaattttccc tttacagatg
    88441 aagagataca gcagcgttcc aataccgtta aataacagta cagtcgccgg gttaatatga
    88501 aataagacgg gcaccaggac ggttgcacca aacatggcga acaaatgttg caaactaagc
    88561 gggattgtct gtaaaagtgg cggtctttca ctcaccccga tagcacggcg cgtcatagta
    88621 ttatcctctg tattatgtgt tataggcgct ttactcaaaa aaaagccgac tcttaaagtc
    88681 ggctttaatt atttttattc tttatttcgt accaaagatt ttgtcaccgg catcgccgag
    88741 gcccggaata atgtatccgt gctcgttcag tccctggtca atcgatgcag tatacagttc
    88801 gacgtccggg tgcgcttttt ccagcgcagc gataccttct ggcgcagcga ccagcaccag
    88861 aactttgatg ctgctgcagc ccgctttttt cagcaggtcg atggtcgcga taacggaacc
    88921 accggttgcc agcattgggt caacgatcag cgccatacgc tcatcgatgt tagaaaccag
    88981 tttctggaag tacggtaccg gctccagcgt ttcttcatta cggtacatac cgacaacgct
    89041 gatgcgcgcg ctcggaacgt tttccagcac accgtccatc ataccaagac ccgcacgcag
    89101 aattggcaca acggtaattt tcttaccttt gatctggtcg atttctaccg ggccgttcca
    89161 gccttcgata gttacttttt ccgtttcgag gtcggcggtc gcttcgtaag tcagcaggct
    89221 acccacttcg gaagcgagtt cgcgaaagcg cttggtgctg atatcttgct cacgcatcag
    89281 tcccagcttg tgtttgacga gtgggtgttt gacttccacg atcttcatac tctttctcct
    89341 ttgaggggca gccacaaaaa aatcgacgga ttatacctcc tttcttcagg gcggcaatat
    89401 tattttcgtt gactttagtc aaaatgataa cagtttgaga taaagttatt ttatattcaa
    89461 atggttatga aagaagatta ttccatccga aaactaacct ttgccctggc acaagtcttc
    89521 tttcgccgcg cgcccgggga aaagacgtgc aaaaaggttg tgtaaaacag tctcgcaaac
    89581 gtttgctttc cctgttagaa ttgcgccgaa ttttattttt ctaccgcaag taacgcgtgg
    89641 ggacccaagc agtgaccgat aaaacctctc ttagctacaa agatgccggt gttgatattg
    89701 acgcgggtaa tgctctggtt ggaagaatca aaggcgtagt gaagaaaacg cgtcgtccgg
    89761 aagtgatggg cggtctgggc ggcttcggtg cgctgtgtgc attgccgcaa aaatatcgtg
    89821 aacccgtgct ggtttccggc actgacggcg taggtaccaa gctgcgtctg gcaatggact
    89881 taaaacgtca cgacaccatt ggtattgatc tggtcgccat gtgcgttaat gacctggtgg
    89941 tgcaaggtgc ggaaccgctg tttttcctcg actattacgc aaccggaaaa ctggatgttg
    90001 ataccgcttc agcggtgatc agcggcatcg cggaaggttg tctgcaatcg ggctgttctc
    90061 tggtgggtgg cgaaacggca gaaatgccgg ggatgtatca cggtgaagat tacgatgtcg
    90121 cgggtttctg cgtgggcgtg gtagaaaaat cagaaatcat cgacggctct aaagtcagcg
    90181 acggcgatgt gctgattgca ctcggttcca gcggtccgca ctcgaacggc tattcgctgg
    90241 tgcgcaaaat tcttgaagtc agcggttgtg atccgcaaac caccgaactt gatggtaagc
    90301 cattagccga tcatctgctg gcaccgaccc gcatttacgt gaagtcagtg ctggagttga
    90361 ttgaaaaggt cgatgtgcat gccattgcgc acctgaccgg cggcggcttc tgggaaaaca
    90421 ttccgcgcgt attgccagat aatacccagg cagtgattga tgaatcttcc tggcagtggc
    90481 cggaagtgtt caactggctg caaacggcag gtaacgttga gcgccatgaa atgtatcgca
    90541 ccttcaactg cggcgtcggg atgattatcg ccctgcctgc tccggaagtg gacaaagccc
    90601 tcgccctgct aaatgccaac ggtgaaaacg cgtggaaaat cggtatcatc aaagcctctg
    90661 attccgaaca acgcgtggtt atcgaataat gaatattgtg gtgcttattt ccggcaacgg
    90721 aagtaattta caggcaatta ttgacgcctg taaaaccaac aaaattaaag gcaccgtacg
    90781 ggcagttttc agcaataagg ccgacgcgtt cggccttgaa cgcgcccgcc aggcgggtat
    90841 tgcaacgcat acgctcatcg ccagcgcgtt tgacagtcgt gaagcctatg accgggagtt
    90901 gattcatgaa atcgacatgt acgcacccga tgtggtcgtg ctggctggtt ttatgcgcat
    90961 tctcagcccg gcgtttgtct cccactatgc cgggcgtttg ctgaacattc acccttctct
    91021 gctgccgaaa tatcccggat tacacaccca tcgtcaggcg ctggaaaatg gcgatgaaga
    91081 gcacggtaca tcggtgcatt tcgtcaccga tgaactggac ggtggcccgg ttattttaca
    91141 ggcgaaagtc ccggtatttg ctggtgatac ggaagatgac atcaccgccc gcgtgcaaac
    91201 ccaggaacac gctatttatc cactggtgat tagctggttt gccgatggtc gtctgaaaat
    91261 gcacgaaaac gccgcgtggc tggatggtca acgtctgccg ccgcagggct acgctgccga
    91321 cgagtaatgc ccccgtagtt aaagcgccag ctctgccgct ggcgtttttc aattcacctg
    91381 taaatcgcaa gctccagcag tttttttccc ccttttctgg catagttgga catctgccaa
    91441 tattgctcgc cataatatcc aggcagtgtc ccgtgaataa aacggagtaa aagtggtaat
    91501 gggtcaggaa aagctataca tcgaaaaaga gctcagttgg ttatcgttca atgaacgcgt
    91561 gcttcaggaa gcggcggaca aatctaaccc gctgattgaa aggatgcgtt tcctggggat
    91621 ctattccaat aaccttgatg agttctataa agtccgcttc gctgaactga agcgacgcat
    91681 cattattagc gaagaacaag gctccaacgc tcattcccgc catttactgg gcaaaattca
    91741 gtcccgggtg ctgaaagccg atcaggaatt cgacggcctc tacaacgagc tattgctgga
    91801 gatggcgcgc aaccagatct tcctgattaa tgaacgccag ctctccgtca atcaacaaaa
    91861 ctggctgcgt cattatttta agcagtatct gcgtcagcac attacgccga ttttaatcaa
    91921 tcctgacact gacttagtgc agttcctgaa agatgattac acctatctgg cggtggaaat
    91981 tatccgtagc gatagcatcc gttacgcgct gctggagatc ccatcagata aagtgccgcg
    92041 ctttgtgaat ttaccgccag aagcgccgcg tcgacgcaag ccgatgattc ttctggataa
    92101 cattctgcgt tactgccttg atgatatttt caaaggcttc tttgattatg acgcgctgaa
    92161 tgcctattca atgaagatga cccgcgatgc cgaatacgat ttagtgcatg agatggaagc
    92221 cagcctgatg gagttgatgt cttccagtct caagcagcgt ttaactgctg agccggtgcg
    92281 ttttgtttat cagcgcgata tgcccaatgc gctggttgaa gtgttacgcg aaaaactgac
    92341 tatttcccgc tacgactcca tcgtccccgg cggtcgttat cataatttta aagactttat
    92401 taatttcccc aatgtcggca aagccaatct ggtgaacaaa ccactgccgc gtttacgcca
    92461 tatttggttt gataaagccc agttccgcaa tggttttgat gccattcgcg aacgcgatgt
    92521 gttgctctat tatccttatc acacctttga gcatgtgctg gaactgctgc gtcaggcttc
    92581 gttcgacccg agcgtactgg cgattaaaat taacatttac cgcgtggcga aagattcacg
    92641 catcatcgac tcgatgatcc acgccgcaca taacggtaag aaagtcactg tggtggttga
    92701 gttacaggcg cgtttcgacg aagaagccaa cattcactgg gcgaagcgcc tgaccgaagc
    92761 aggcgtgcac gttatcttct ctgcgccggg gctgaaaatt cacgccaaac tgttcctgat
    92821 ttcacgtaaa gaaaacggtg aagtggtccg ttacgcacac atcgggaccg ggaactttaa
    92881 cgaaaaaacc gcgcgtcttt atactgacta ttcgttgctg accgcagatg cgcgtatcac
    92941 caacgaagta cggcgggtat ttaactttat tgaaaaccca taccgcccgg tgacatttga
    93001 ttatttaatg gtgtcaccgc aaaactctcg ccgtctgtta tatgaaatgg tagaccgcga
    93061 aatcgccaac gcgcagcaag ggctgcccag tggtatcacc ctgaagctaa ataaccttgt
    93121 cgataaaggc ctggttgatc gtctgtatgc ggcctccagc tccggcgtac cggttaatct
    93181 gctggttcgc ggaatgtgtt cgctgatccc caatctggaa ggcattagcg acaacattcg
    93241 tgccatcagt attgttgacc gttaccttga acatgaccgg gtttatattt ttgaaaatgg
    93301 cggcgataaa aaggtctacc tttcttccgc cgactggatg acgcgcaata ttgattatcg
    93361 tattgaagtg gcgacaccgc tgctcgatcc gcgcctgaag cagcgggtgc tggacatcat
    93421 cgacatattg ttcagcgata cggtcaaagc acgttatatc gataaagaac tcagtaatcg
    93481 ctacgttccc cgcggcaatc gccgcaaagt acgggcgcag ttggcgattt acgactacat
    93541 caaatcactc gaacaacctg aataacccta tgccaataca cgataaatcc cctcgtccgc
    93601 aggagtttgc tgcggtcgat cttggttcaa acagttttca catggtcata gcccgtgtgg
    93661 tagatggtgc catgcagatt attggccgcc tgaaacagcg ggtgcatctg gcggacggcc
    93721 tggggccaga taatatgttg agtgaagagg caatgacgcg cggtttgaac tgtctgtcgc
    93781 tgtttgccga acggctacaa gggttttctc ctgccagcgt ctgtatagtc ggcacccata
    93841 cgctgcgtca ggcgctgaac gccactgact ttctgaaacg cgcggaaaag gtcattccct
    93901 acccgattga aattatttcc ggtaatgaag aagcccgtct gatttttatg ggcgtggaac
    93961 atacccaacc ggaaaaaggt cgcaaactgg ttattgatat tggcggcgga tctacggaac
    94021 tggtgattgg tgaaaatttc gaacctattc tcgttgaaag ccgccggatg ggttgtgtca
    94081 gctttgccca gctttatttt cctggcgggg tcatcaataa agagaatttt cagcgcgctc
    94141 gcatggcggc agcacaaaaa ctggaaactt taacctggca attccgtatt cagggctgga
    94201 acgttgcaat gggcgcttcc ggtaccataa aagccgccca tgaagtgtta atggaaatgg
    94261 gcgagaaaga cgggataatt accccggaac gtctggaaaa actggtaaaa gaaattttac
    94321 gtcaccgtaa tttcgcatcg ctgagtttac cgggtctttc cgaagagcgg aaaacagtct
    94381 tcgttcctgg actggcgatt ttatgtggtg tgtttgatgc tttagccatc cgtgaactgc
    94441 gcctttctga cggggcgtta cgcgaaggcg tactgtatga aatggaagga cgtttccgtc
    94501 atcaggatgt gcgtagtcgc accgccagca gcctcgccaa ccagtatcac atagacagcg
    94561 aacaggcccg acgagtgctg gataccacta tgcaaatgta cgaacagtgg cgggaacagc
    94621 aaccgaagct ggcgcatccg caactggagg cgctactgcg atgggccgcc atgctgcatg
    94681 aggtcgggtt gaatatcaac cacagcggtt tgcatcgcca ctccgcttat attctgcaaa
    94741 acagtgactt gccgggtttt aatcaggaac agcagctgat gatggcgaca ctggtgcgct
    94801 atcaccgtaa agcgattaag ctcgacgatc tgccgcgctt taccttgttt aagaagaaac
    94861 agttcctgcc actgatacag ctattgcgcc ttggcgtatt actaaacaat caacgtcagg
    94921 caaccaccac accgccaaca ttgacactga ttactgatga cagtcactgg acactgcgtt
    94981 tcccgcatga ctggtttagt cagaatgcgc tggtactgct tgatctggaa aaggagcaag
    95041 aatactggga aggcgtggct ggctggcggt tgaaaattga agaagaaagt accccagaaa
    95101 tcgccgctta atgctttgcg ggccgacgag aaatgtcggc ccgcattatt caggcacttt
    95161 cgcgaatggg ttcgatttca ttcagcgtat caattaacgg ttgcggctta ccaataagat
    95221 aaccctgcat ataatcgatc cccaaagaga gcaccgcctc gcggatctct tcgttttcaa
    95281 cgtactctgc cactacccgc attttcttca ttcgcgccag atggcaaatc gacgccacaa
    95341 tctgataatc cagactattt gacacaatat tgcggataaa gctgccgtca attttaagca
    95401 gatcggcatt cacattttta agccgtgcat agctggcgta gccggtgccg aaatcatcaa
    95461 tcgcaatctg gcagcctaat tcctgaagat gctgcaaggt gatttgcgcc tgcttaacat
    95521 tggtcagagc attactttcg gtgacttcaa aaataagttg ccacgcttca atctgatatt
    95581 tagccagcaa ctgactgact tcaacaggaa aacgagcctg acataccgag gttggagaca
    95641 gattaatagc aaaacggtga gcgggcatct tcgctctgtt ttcagccata aattgcagcg
    95701 tatgctcaat gacccacatg tcgatactcg acgataaacc aaattcgtgc gcgaccggca
    95761 agaagctacc ggggccgatc agttcatcat tctcaccttt catgcgcaga agaatttcat
    95821 ggtaaacatc accacgcata ccggtaatcg gctgggccat caggaaaaaa tggttgtgtt
    95881 ccagcgcctg ctgtagtcga ttcatcatcg cgactttatc tttcaattcg cgttgcaaat
    95941 acattgcccc gcaacgctgc atattttccg gggcgttggt cacgatggaa agttcggcga
    96001 ccgtatttag ctctcccagc agcaggtaga tatgattcac tggcgagcgc acatagcagt
    96061 aactgacgcc aatctgcggt tgcatcggca tcccatccca aaagaaacga aattgcttga
    96121 gatgactatc cagtgcggta atgcgctcct ggtgcgattc tgtattcagt cgcagcgcga
    96181 gatcgttacc cgaaagctga taaacatctt cacccggttc cagcaagggt gacagccagt
    96241 gagaaagttt ttgctt
//
LOCUS       DAJTSA010000002        95540 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000002 DAJTSA010000000
VERSION     DAJTSA010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 95540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95540
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.002"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(87..1337)
                     /gene="icd"
                     /locus_tag="OO369_000098"
     CDS             complement(87..1337)
                     /gene="icd"
                     /locus_tag="OO369_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR5980522.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1509..2162
                     /gene="rluE"
                     /locus_tag="OO369_000099"
     CDS             1509..2162
                     /gene="rluE"
                     /locus_tag="OO369_000099"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR5980523.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2172..2633
                     /gene="nudJ"
                     /locus_tag="OO369_000100"
     CDS             2172..2633
                     /gene="nudJ"
                     /locus_tag="OO369_000100"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR5980524.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2687..3793
                     /gene="mnmA"
                     /locus_tag="OO369_000101"
     CDS             2687..3793
                     /gene="mnmA"
                     /locus_tag="OO369_000101"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR5980525.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3829..4470
                     /gene="hflD"
                     /locus_tag="OO369_000102"
     CDS             3829..4470
                     /gene="hflD"
                     /locus_tag="OO369_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR5980526.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4474..5844
                     /gene="purB"
                     /locus_tag="OO369_000103"
     CDS             4474..5844
                     /gene="purB"
                     /locus_tag="OO369_000103"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR5980527.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6013..6684
                     /gene="phoP"
                     /locus_tag="OO369_000104"
     CDS             6013..6684
                     /gene="phoP"
                     /locus_tag="OO369_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR5980528.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6684..8144
                     /gene="phoQ"
                     /locus_tag="OO369_000105"
     CDS             6684..8144
                     /gene="phoQ"
                     /locus_tag="OO369_000105"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR5980529.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8941..9222)
                     /locus_tag="OO369_000106"
     CDS             complement(8941..9222)
                     /locus_tag="OO369_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5980530.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9236..10897)
                     /locus_tag="OO369_000107"
     CDS             complement(9236..10897)
                     /locus_tag="OO369_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5980531.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10881..11207)
                     /locus_tag="OO369_000108"
     CDS             complement(10881..11207)
                     /locus_tag="OO369_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR5980532.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYSVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11263..11445)
                     /locus_tag="OO369_000109"
     CDS             complement(11263..11445)
                     /locus_tag="OO369_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5980533.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11429..11869)
                     /locus_tag="OO369_000110"
     CDS             complement(11429..11869)
                     /locus_tag="OO369_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5980534.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11869..12165)
                     /locus_tag="OO369_000111"
     CDS             complement(11869..12165)
                     /locus_tag="OO369_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR5980535.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(12162..12500)
                     /locus_tag="OO369_000112"
     CDS             complement(12162..12500)
                     /locus_tag="OO369_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5980536.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12497..13708)
                     /locus_tag="OO369_000113"
     CDS             complement(12497..13708)
                     /locus_tag="OO369_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5980537.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13710..14282)
                     /locus_tag="OO369_000114"
     CDS             complement(13710..14282)
                     /locus_tag="OO369_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR5980538.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14322..15479)
                     /locus_tag="OO369_000115"
                     /pseudo
     CDS             complement(14322..15479)
                     /locus_tag="OO369_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15780..16004)
                     /locus_tag="OO369_000116"
     CDS             complement(15780..16004)
                     /locus_tag="OO369_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5980539.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(16146..16307)
                     /locus_tag="OO369_000117"
     CDS             complement(16146..16307)
                     /locus_tag="OO369_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5980540.1"
                     /translation="MTAQIAAYGQLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(16317..16622)
                     /locus_tag="OO369_000118"
     CDS             complement(16317..16622)
                     /locus_tag="OO369_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5980541.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16619..16897)
                     /locus_tag="OO369_000119"
     CDS             complement(16619..16897)
                     /locus_tag="OO369_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5980542.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(17186..18934)
                     /locus_tag="OO369_000120"
     CDS             complement(17186..18934)
                     /locus_tag="OO369_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5980543.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18931..19230)
                     /locus_tag="OO369_000121"
     CDS             complement(18931..19230)
                     /locus_tag="OO369_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5980544.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(19236..19469)
                     /locus_tag="OO369_000122"
     CDS             complement(19236..19469)
                     /locus_tag="OO369_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5980545.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19471..19691)
                     /locus_tag="OO369_000123"
                     /pseudo
     CDS             complement(19471..19691)
                     /locus_tag="OO369_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19684..19905)
                     /locus_tag="OO369_000124"
     CDS             complement(19684..19905)
                     /locus_tag="OO369_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5980546.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19895..20095)
                     /locus_tag="OO369_000125"
     CDS             complement(19895..20095)
                     /locus_tag="OO369_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR5980547.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            20225..20404
                     /locus_tag="OO369_000126"
     CDS             20225..20404
                     /locus_tag="OO369_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5980548.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20529..20858
                     /locus_tag="OO369_000127"
     CDS             20529..20858
                     /locus_tag="OO369_000127"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5980549.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20908..21096)
                     /locus_tag="OO369_000128"
     CDS             complement(20908..21096)
                     /locus_tag="OO369_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5980550.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21471..22700)
                     /locus_tag="OO369_000129"
     CDS             complement(21471..22700)
                     /locus_tag="OO369_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5980551.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22949..24070
                     /gene="roxA"
                     /locus_tag="OO369_000130"
                     /gene_synonym="ycfD"
     CDS             22949..24070
                     /gene="roxA"
                     /locus_tag="OO369_000130"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR5980552.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(24119..25345)
                     /gene="pepT"
                     /locus_tag="OO369_000131"
     CDS             complement(24119..25345)
                     /gene="pepT"
                     /locus_tag="OO369_000131"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR5980553.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25595..26731
                     /gene="potA"
                     /locus_tag="OO369_000132"
     CDS             25595..26731
                     /gene="potA"
                     /locus_tag="OO369_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR5980554.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26715..27572
                     /gene="potB"
                     /locus_tag="OO369_000133"
     CDS             26715..27572
                     /gene="potB"
                     /locus_tag="OO369_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR5980555.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27569..28363
                     /gene="potC"
                     /locus_tag="OO369_000134"
     CDS             27569..28363
                     /gene="potC"
                     /locus_tag="OO369_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR5980556.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            28360..29406
                     /gene="potD"
                     /locus_tag="OO369_000135"
     CDS             28360..29406
                     /gene="potD"
                     /locus_tag="OO369_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR5980557.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29562..30383)
                     /gene="cobB"
                     /locus_tag="OO369_000136"
     CDS             complement(29562..30383)
                     /gene="cobB"
                     /locus_tag="OO369_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR5980558.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30399..31310)
                     /gene="nagK"
                     /locus_tag="OO369_000137"
     CDS             complement(30399..31310)
                     /gene="nagK"
                     /locus_tag="OO369_000137"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR5980559.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(31339..32583)
                     /gene="lolE"
                     /locus_tag="OO369_000138"
     CDS             complement(31339..32583)
                     /gene="lolE"
                     /locus_tag="OO369_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR5980560.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASRYPARRASNIDPARVLSGQ"
     gene            complement(32583..33284)
                     /gene="lolD"
                     /locus_tag="OO369_000139"
     CDS             complement(32583..33284)
                     /gene="lolD"
                     /locus_tag="OO369_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR5980561.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(33277..34476)
                     /gene="lolC"
                     /locus_tag="OO369_000140"
     CDS             complement(33277..34476)
                     /gene="lolC"
                     /locus_tag="OO369_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR5980562.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34738..35811
                     /locus_tag="OO369_000141"
     CDS             34738..35811
                     /locus_tag="OO369_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5980563.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35939..39385
                     /gene="mfd"
                     /locus_tag="OO369_000142"
     CDS             35939..39385
                     /gene="mfd"
                     /locus_tag="OO369_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR5980564.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39528..40490
                     /gene="ldtC"
                     /locus_tag="OO369_000143"
     CDS             39528..40490
                     /gene="ldtC"
                     /locus_tag="OO369_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCR5980565.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40573..40785)
                     /locus_tag="OO369_000144"
     CDS             complement(40573..40785)
                     /locus_tag="OO369_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5980566.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            41070..41702
                     /gene="comR"
                     /locus_tag="OO369_000145"
     CDS             41070..41702
                     /gene="comR"
                     /locus_tag="OO369_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR5980567.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(41764..42303)
                     /locus_tag="OO369_000146"
     CDS             complement(41764..42303)
                     /locus_tag="OO369_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5980568.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(42530..43834)
                     /gene="ndh"
                     /locus_tag="OO369_000147"
     CDS             complement(42530..43834)
                     /gene="ndh"
                     /locus_tag="OO369_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR5980569.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(44242..44784)
                     /locus_tag="OO369_000148"
     CDS             complement(44242..44784)
                     /locus_tag="OO369_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5980570.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(44807..45832)
                     /gene="nagZ"
                     /locus_tag="OO369_000149"
     CDS             complement(44807..45832)
                     /gene="nagZ"
                     /locus_tag="OO369_000149"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR5980571.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(45843..46667)
                     /gene="thiK"
                     /locus_tag="OO369_000150"
     CDS             complement(45843..46667)
                     /gene="thiK"
                     /locus_tag="OO369_000150"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR5980572.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(46648..47289)
                     /gene="lpoB"
                     /locus_tag="OO369_000151"
     CDS             complement(46648..47289)
                     /gene="lpoB"
                     /locus_tag="OO369_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR5980573.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPDPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(47304..47681)
                     /locus_tag="OO369_000152"
     CDS             complement(47304..47681)
                     /locus_tag="OO369_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR5980574.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(47684..48043)
                     /gene="hinT"
                     /locus_tag="OO369_000153"
     CDS             complement(47684..48043)
                     /gene="hinT"
                     /locus_tag="OO369_000153"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR5980575.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            48174..48361
                     /locus_tag="OO369_000154"
                     /pseudo
     CDS             48174..48361
                     /locus_tag="OO369_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012138106.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            48394..50579
                     /gene="fhuE"
                     /locus_tag="OO369_000155"
                     /pseudo
     CDS             48394..50579
                     /gene="fhuE"
                     /locus_tag="OO369_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(50639..52072)
                     /gene="ptsG"
                     /locus_tag="OO369_000156"
     CDS             complement(50639..52072)
                     /gene="ptsG"
                     /locus_tag="OO369_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR5980576.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(52367..53164)
                     /locus_tag="OO369_000157"
     CDS             complement(52367..53164)
                     /locus_tag="OO369_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5980577.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(53175..54179)
                     /gene="holB"
                     /locus_tag="OO369_000158"
     CDS             complement(53175..54179)
                     /gene="holB"
                     /locus_tag="OO369_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR5980578.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(54176..54817)
                     /locus_tag="OO369_000159"
     CDS             complement(54176..54817)
                     /locus_tag="OO369_000159"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR5980579.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(54807..55829)
                     /gene="yceG"
                     /locus_tag="OO369_000160"
     CDS             complement(54807..55829)
                     /gene="yceG"
                     /locus_tag="OO369_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR5980580.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(55832..56641)
                     /gene="pabC"
                     /locus_tag="OO369_000161"
     CDS             complement(55832..56641)
                     /gene="pabC"
                     /locus_tag="OO369_000161"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR5980581.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(56761..58002)
                     /gene="fabF"
                     /locus_tag="OO369_000162"
     CDS             complement(56761..58002)
                     /gene="fabF"
                     /locus_tag="OO369_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR5980582.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(58089..58325)
                     /gene="acpP"
                     /locus_tag="OO369_000163"
     CDS             complement(58089..58325)
                     /gene="acpP"
                     /locus_tag="OO369_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5980583.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(58536..59270)
                     /gene="fabG"
                     /locus_tag="OO369_000164"
     CDS             complement(58536..59270)
                     /gene="fabG"
                     /locus_tag="OO369_000164"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR5980584.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(59283..60212)
                     /gene="fabD"
                     /locus_tag="OO369_000165"
     CDS             complement(59283..60212)
                     /gene="fabD"
                     /locus_tag="OO369_000165"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR5980585.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(60228..61181)
                     /gene="fabH"
                     /locus_tag="OO369_000166"
     CDS             complement(60228..61181)
                     /gene="fabH"
                     /locus_tag="OO369_000166"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR5980586.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(61249..62319)
                     /gene="plsX"
                     /locus_tag="OO369_000167"
     CDS             complement(61249..62319)
                     /gene="plsX"
                     /locus_tag="OO369_000167"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR5980587.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(62400..62573)
                     /gene="rpmF"
                     /locus_tag="OO369_000168"
     CDS             complement(62400..62573)
                     /gene="rpmF"
                     /locus_tag="OO369_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR5980588.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(62625..63146)
                     /gene="yceD"
                     /locus_tag="OO369_000169"
     CDS             complement(62625..63146)
                     /gene="yceD"
                     /locus_tag="OO369_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR5980589.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            63345..63929
                     /gene="yceF"
                     /locus_tag="OO369_000170"
     CDS             63345..63929
                     /gene="yceF"
                     /locus_tag="OO369_000170"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR5980590.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(64041..65000)
                     /gene="rluC"
                     /locus_tag="OO369_000171"
     CDS             complement(64041..65000)
                     /gene="rluC"
                     /locus_tag="OO369_000171"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR5980591.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            65573..68764
                     /gene="rne"
                     /locus_tag="OO369_000172"
     CDS             65573..68764
                     /gene="rne"
                     /locus_tag="OO369_000172"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR5980592.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(68961..69903)
                     /gene="flgL"
                     /locus_tag="OO369_000173"
                     /pseudo
     CDS             complement(68961..69903)
                     /gene="flgL"
                     /locus_tag="OO369_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(69915..71557)
                     /gene="flgK"
                     /locus_tag="OO369_000174"
                     /pseudo
     CDS             complement(69915..71557)
                     /gene="flgK"
                     /locus_tag="OO369_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(71623..72564)
                     /gene="flgJ"
                     /locus_tag="OO369_000175"
     CDS             complement(71623..72564)
                     /gene="flgJ"
                     /locus_tag="OO369_000175"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR5980593.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(72564..73661)
                     /gene="flgI"
                     /locus_tag="OO369_000176"
     CDS             complement(72564..73661)
                     /gene="flgI"
                     /locus_tag="OO369_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR5980594.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(73673..74371)
                     /gene="flgH"
                     /locus_tag="OO369_000177"
     CDS             complement(73673..74371)
                     /gene="flgH"
                     /locus_tag="OO369_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR5980595.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(74424..75206)
                     /gene="flgG"
                     /locus_tag="OO369_000178"
     CDS             complement(74424..75206)
                     /gene="flgG"
                     /locus_tag="OO369_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR5980596.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(75344..76095)
                     /gene="flgF"
                     /locus_tag="OO369_000179"
                     /pseudo
     CDS             complement(75344..76095)
                     /gene="flgF"
                     /locus_tag="OO369_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(76115..77323)
                     /gene="flgE"
                     /locus_tag="OO369_000180"
     CDS             complement(76115..77323)
                     /gene="flgE"
                     /locus_tag="OO369_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR5980597.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(77348..78043)
                     /gene="flgD"
                     /locus_tag="OO369_000181"
     CDS             complement(77348..78043)
                     /gene="flgD"
                     /locus_tag="OO369_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR5980598.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(78055..78458)
                     /gene="flgC"
                     /locus_tag="OO369_000182"
                     /pseudo
     CDS             complement(78055..78458)
                     /gene="flgC"
                     /locus_tag="OO369_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(78462..78878)
                     /gene="flgB"
                     /locus_tag="OO369_000183"
     CDS             complement(78462..78878)
                     /gene="flgB"
                     /locus_tag="OO369_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR5980599.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            79033..79692
                     /gene="flgA"
                     /locus_tag="OO369_000184"
     CDS             79033..79692
                     /gene="flgA"
                     /locus_tag="OO369_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR5980600.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            79768..80061
                     /gene="flgM"
                     /locus_tag="OO369_000185"
     CDS             79768..80061
                     /gene="flgM"
                     /locus_tag="OO369_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR5980601.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            80066..80482
                     /gene="flgN"
                     /locus_tag="OO369_000186"
     CDS             80066..80482
                     /gene="flgN"
                     /locus_tag="OO369_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR5980602.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(80522..82057)
                     /gene="murJ"
                     /locus_tag="OO369_000187"
     CDS             complement(80522..82057)
                     /gene="murJ"
                     /locus_tag="OO369_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR5980603.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(82257..83090)
                     /locus_tag="OO369_000188"
     CDS             complement(82257..83090)
                     /locus_tag="OO369_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5980604.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     V"
     gene            complement(83092..83739)
                     /locus_tag="OO369_000189"
     CDS             complement(83092..83739)
                     /locus_tag="OO369_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR5980605.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(83750..84334)
                     /gene="rimJ"
                     /locus_tag="OO369_000190"
     CDS             complement(83750..84334)
                     /gene="rimJ"
                     /locus_tag="OO369_000190"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5980606.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            84570..85778
                     /gene="mdtH"
                     /locus_tag="OO369_000191"
     CDS             84570..85778
                     /gene="mdtH"
                     /locus_tag="OO369_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR5980607.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            85842..86489
                     /gene="grxB"
                     /locus_tag="OO369_000192"
     CDS             85842..86489
                     /gene="grxB"
                     /locus_tag="OO369_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR5980608.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            86623..87183
                     /locus_tag="OO369_000193"
     CDS             86623..87183
                     /locus_tag="OO369_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5980609.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            87289..88335
                     /gene="pyrC"
                     /locus_tag="OO369_000194"
     CDS             87289..88335
                     /gene="pyrC"
                     /locus_tag="OO369_000194"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR5980610.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            88409..88654
                     /gene="dinI"
                     /locus_tag="OO369_000195"
     CDS             88409..88654
                     /gene="dinI"
                     /locus_tag="OO369_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR5980611.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            88944..89198
                     /gene="bssS"
                     /locus_tag="OO369_000196"
     CDS             88944..89198
                     /gene="bssS"
                     /locus_tag="OO369_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR5980612.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            89313..90431
                     /gene="solA"
                     /locus_tag="OO369_000197"
     CDS             89313..90431
                     /gene="solA"
                     /locus_tag="OO369_000197"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR5980613.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            90479..90592
                     /locus_tag="OO369_000198"
     CDS             90479..90592
                     /locus_tag="OO369_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR5980614.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            90853..91419
                     /locus_tag="OO369_000199"
     CDS             90853..91419
                     /locus_tag="OO369_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR5980615.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMATLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            91423..91998
                     /locus_tag="OO369_000200"
     CDS             91423..91998
                     /locus_tag="OO369_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR5980616.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(92040..93092)
                     /locus_tag="OO369_000201"
     CDS             complement(92040..93092)
                     /locus_tag="OO369_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR5980617.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            93317..94237
                     /gene="lpxL"
                     /locus_tag="OO369_000202"
     CDS             93317..94237
                     /gene="lpxL"
                     /locus_tag="OO369_000202"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR5980618.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            94409..94606
                     /locus_tag="OO369_000203"
     CDS             94409..94606
                     /locus_tag="OO369_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR5980619.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            94630..>95540
                     /locus_tag="OO369_000204"
     CDS             94630..>95540
                     /locus_tag="OO369_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5980620.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGII"
BASE COUNT        22984 a        25317 c        23576 g        23663 t
ORIGIN      
        1 atgctcgata tgctattaat aatatccaca taaataaaac aacgggcgtg ttatacgccc
       61 gtttaaatat ttaacacatg tagtgattac atattcttga tgatcgcatc accaaactct
      121 gaacatttca gcagtttagc gccttccatc agacgctcga agtcataggt tacggtcttg
      181 gcgttgatcg cgccttccat acctttaaca atcaggtctg cggcttcagt ccagcccata
      241 tggcgtaaca tcatctcagc ggagagaata atagagccag ggtttacttt gtcctgaccg
      301 gcgtatttcg gcgcagtacc gtgggtggct tcaaacaggg cgcattcgtc accgatgttt
      361 gctccagggg cgataccgat accgccaacc tgcgctgcca gagcgtcaga aatgtagtca
      421 ccgttcaggt tcatacaggc gataacatca tattcagccg gacgcagcag gatctgttgc
      481 aggaatgcat cagcaatcac gtctttaatg acgatctctt tgccggtatt cgggttttta
      541 actttcagcc acgggccgcc gtcgatcagt tcaccgccaa attcttcacg cgccagctgg
      601 tagccccagt ctttaaacgc gccttcggtg aacttcatga tgttgccttt gtgcaccagg
      661 gtcacagagt cacgatcgtt agcaattgcg tattcgatcg ctgcacgaac cagacgtttg
      721 gtgccttctt cagaacacgg cttaataccg ataccgcaat gttccgggaa gcgaattttc
      781 ttcacgccca tctcttcacg caggaattta atcactttct cggcgtcggc agagtcagct
      841 ttccattcga tacccgcata aatgtcttcc gagttttcac ggaagataac catatcggtc
      901 agttcagggt gtttaaccgg gcttggagtg ccctgatagt aacgtaccgg acgcaggcag
      961 atgtagagat ccagttcctg acgcagggca acgttgagag aacgaatacc gccaccaacc
     1021 ggagtggtca gcgggccttt aatggcaacg cgatattcac gaatcagatc aagggtttca
     1081 gcaggcagcc agacatcctg accataaacc tgtgtggatt tttcaccggt gtaaatttcc
     1141 atccaggaga ttttacgctc gcctttatag gctttctcga ctgcagcgtc gaccactttc
     1201 agcatggctg gggttacatc tacaccgatt ccatcacctt caatgtaagg gataatcgga
     1261 ttttcaggaa cgttgagttt gccgttttgc agggtgatct tcttgccttg tgccggaaca
     1321 actactttac tttccattca cctctccttc gagcgctact ggtttgctcg tctgccacca
     1381 cgttgcatat gcgtttgcgt cctgcgatac ggatgcttta gagcaatttt ttgttaatga
     1441 tttgtaattg acttgtcaat actaccctat tgtttgccgt catgaaagat gcgcgttatt
     1501 aggctataat gcggcaattc ataatctctg aaaataccat gcaaaaaact tcttttagaa
     1561 atcaccaggt taagcgattc agctcgcaac gttctaccag gcgtaaacat gaaaaccagc
     1621 ccacgcgtgt gatcctcttc aataaaccct acgatgttct tccgcagttc accgatgaaa
     1681 ccggacgcaa aacattaaaa gaattcatcc cggttcaggg cgtttatgca gcaggtcgcc
     1741 ttgaccgcga tagcgaaggg ttgctggtgc tgaccaataa cggcgcgttg caggcgcgtt
     1801 taacccagcc gggtaaacgc accggaaaaa tctattatgt gcaggtggaa ggtattccca
     1861 cacaagacgc acttgaagcc ttgcgcaatg gcgtaacctt aaatgatggc cctaccctgc
     1921 ccgcaggcgc ggagctggtt gacgaacccg cgtggttatg gccgcggaat ccaccaattc
     1981 gtgaacgcaa aagtattccc accagctggc tgaagatcac cttatatgaa ggacgtaatc
     2041 gccaggtgcg ccgcatgacc gcccatgttg gtttccccac gctgcgactg attcgctatg
     2101 cgatgggaga ttactctttg gataatcttg ccaatggcga atggcgagaa gtgacagatt
     2161 aaggatgcaa aatgtttaaa ccgcacgtta ccgttgcttg cgtggtgcac gcagaaggca
     2221 aatttttagt cgttgaagag acgattaatg gtaaagcgtt atggaaccaa cctgccgggc
     2281 atctggaagc cgatgaaacc ttagtggaag ccgccgcccg tgaactgtgg gaagaaaccg
     2341 gcatcagcgc gcagccgcaa cactttattc gtatgcatca gtggattgcg ccagataaaa
     2401 cgccgttttt gcgtttcctc tttgccattg agcttgagca aatatgcccg acccagcctc
     2461 atgacagcga tatcgactgc tgccgttggg tcagcgccga agaaatttta caggcgtcaa
     2521 atcttcgttc gccgctggtg gcggaaagta ttcgttgtta tcaaagcggg caacgttatc
     2581 cgctggagat gattggcgat tttaactggc cttttacaaa gggtgtcatc taaaaggctg
     2641 cgtgatagaa tacgccgcct tgaagttcaa tgtcgtgagt gatccaatgt ctgaaaccgc
     2701 aaaaaaagta atcgtcggca tgtccggcgg tgtcgattcc tccgtttctg cctggctgtt
     2761 gcaacaacag ggatatcagg tcgaaggcct gtttatgaag aactgggaag aagacgacgg
     2821 tgaggaatat tgcacagcag cagcggatct ggctgatgcc caggcggtct gcgacaagct
     2881 cggcattgaa ctgcacaccg ttaactttgc tgccgagtac tgggacaacg tcttcgaact
     2941 gttccttgcc gaatataaag ccggtcgcac gccgaatccg gatattctgt gcaacaagga
     3001 gatcaaattt aaagccttcc tcgaatttgc cgccgaagat ttaggtgccg attatatcgc
     3061 taccggtcat tacgtacgtc gggccgatgt cgatggcaag agccgcctgc tgcgtggtct
     3121 ggacagcaat aaagaccaga gctacttcct ttatacgctc agccatgagc agattgcgca
     3181 aagcctgttc ccggtaggcg aactggaaaa accgcaggtg cgtaagattg ctgaagatct
     3241 tggtctggtc accgcgaaga aaaaagactc taccggcatt tgcttcattg gcgaacgtaa
     3301 attccgcgag ttcctgggcc gttatctccc ggcgcaaccg ggcaaaatca ttaccgtcga
     3361 tggcgatgaa attggtgagc accaggggct gatgtatcac actctcggtc agcgtaaagg
     3421 tctgggtatc ggtggcacca aagaaggtac cgaagaaccg tggtatgtgg tggacaaaga
     3481 cgtcgaaaac aacattctgg ttgtcgctca gggccatgaa cacccgcggc tgatgtctgt
     3541 cgggttgatt gcccagcagc tgcactgggt cgatcgcgaa ccattcaccg gcactatgcg
     3601 ttgcacggta aaaacccgct atcgccagac cgacattccg tgcaccgtca aggcgctgga
     3661 cgacgatcgc attaaagtga ttttcgatga gccggttgcc gccgtgacgc cgggccagtc
     3721 tgccgtcttc tataacggtg aagtgtgcct cggtggcggt attattgagc agcgtctgcc
     3781 gctgccggtc tgattattat ctttacttaa aacaaggaag cagtgaacgt ggcaaagaat
     3841 tactatgaca tcaccctcgc cctggccggt atttgtcagt cggcacgcct ggtgcaacaa
     3901 ctcgctcacc aggggcattg tgatgccgat gcgctacacg tctcactcaa cagtattatt
     3961 gatatgaacc ccagctcgac gctggcggtt tttggcggta gcgaagccaa cctgcgcgtc
     4021 gggctggaaa ccctgctcgg cgtgctcaat gccagcagtt gccagggctt aaacgccgaa
     4081 ttaacccgct acacactcag cttgatggtg cttgagcgca aactctcctc agcgaaaggc
     4141 gcgctcgaca ctctgggcaa ccggatcaac ggcctgcaac gccagctcga acacttcgat
     4201 ttacagtccg aaacgctgat gagcgcgatg gctgctatct atgttgatgt gatcagcccg
     4261 cttggcccgc gcattcaggt caccggttcc cctgctgtac tgcaaagccc acaagtgcag
     4321 gcgaaagttc gcgcaaccct gctggcaggc attcgcgccg ccgtgctctg gcaccaggtc
     4381 ggcggcggac gtctacaact gatgttttct cgtaatcgcc tgaccactca ggcaaaacaa
     4441 attcttgctc atttaacccc ggagttgtga tctatggaat tatcctcact gaccgccgtt
     4501 tcccctgtcg atggacgcta cggcgataaa gtcagcgcgc tgcgcgggat tttcagcgaa
     4561 tatggtttgc tgaaattccg tgtacaagtt gaagtacgtt ggctgcaaaa actggccgcg
     4621 cacgcagcga tcaaggaagt tcctgctttt gctgccgacg caatcggtta ccttgatgca
     4681 attgtcgcca atttcagcga agaagatgcc gcacgcatca aaaccatcga gcgtaccact
     4741 aaccacgacg ttaaagcggt tgagtatttc ctgaaagaaa aagtggcgga gatcccggaa
     4801 ctgcacgcgg tttctgaatt catccacttt gcctgtactt cggaagatat caataacctc
     4861 tcccacgcat taatgctgaa aaccgcgcgt gatgaagtga tcctgccgta ctggcgtcaa
     4921 ctgattgatg gcattaaaga tctcgccgtt cagtaccgcg atatcccgct gctgtctcgt
     4981 acccacggtc agccagccac gccgtcaacc atcggtaaag agatggcaaa cgtcgcctac
     5041 cgtatggagc gccagcaccg ccagcttaac caggtggaga tcctcggcaa aatcaacggc
     5101 gcggtcggta actataacgc ccacatcgcc gcttacccgg aagttgactg gcatcagttc
     5161 agcgaagagt tcgtcacctc gctgggtatt caatggaacc cgtacactac ccagattgaa
     5221 ccgcacgact acattgccga actgtttgat tgcgttgcgc gcttcaacac cattctgatc
     5281 gactttgacc gtgacgtctg gggttatatc gcccttaacc acttcaaaca gaaaaccatt
     5341 gctggtgaga ttggttcttc caccatgccg cataaagtta acccgatcga cttcgaaaac
     5401 tccgaaggga atctgggcct ttccaacgcg gtattgcagc atctggcaag caaactgccg
     5461 gtttcccgct ggcagcgtga cctgaccgac tccaccgtgc tgcgtaacct cggcgtgggt
     5521 atcggttatg cgctgattgc gtatcaatcc accctgaaag gcgtgagcaa actggaagtg
     5581 aaccgtgacc atctgctgga tgagctggat cacaactggg aagtgctggc agaaccaatc
     5641 cagacagtta tgcgtcgcta tggcatcgaa aaaccgtacg agaagctgaa agagcttact
     5701 cgcggtaagc gcgttgacgc cgaaggcatg aagcagttta tcgatggtct ggcgttgcca
     5761 gaagaagaga aagcccgcct gaaagcgatg acgccggcta actatattgg tcgagctatc
     5821 acgatggttg atgagctgaa ataagcctca aatcagtgcc ggatggcgat gctgtccggc
     5881 ctgcttatta agattatcca ctttttcttt tttcacttta cctcccctcc ccgctggttt
     5941 atttaatgtt tacccccata aacacataat cgcgttacac tattttaata attaagacag
     6001 ggagaaataa aaatgcgcgt actggttgtt gaagacaatg cgttgttacg tcaccacctt
     6061 aaagttcaga ttcaggatgc tggtcatcag gtcgatgacg cagaagatgc caaagaagcc
     6121 gattattatc tcaatgaaca tttaccggat attgcgattg tcgatctcgg attgcctgac
     6181 gaggacggtc tgtcactgat tcgccgctgg cgtagcaacg atgtttcact gccgattctg
     6241 gtattaaccg cccgtgaaag ctggcaggac aaagtcgaag tattaagtgc cggtgctgat
     6301 gattacgtga ctaaaccgtt tcatattgaa gaggtgatgg cgcgaatgca ggcattaatg
     6361 cggcgtaata gcggtctggc ttcacaggtc atttcgctcc ccccgtttca ggttgatctc
     6421 tctcgccgtg aattatctat taatgacgaa gtgatcaaac tgaccgcgtt cgaatacacc
     6481 attatggaaa cgttgatacg caataatggc aaagtggtca gcaaagattc gttaatgcta
     6541 caactctatc cggatgcgga gctgcgggaa agccatacca ttgatgtact gatgggacgt
     6601 ctgcgcaaaa aaattcaggc acaatatccg caagaagtca ttaccaccgt tcgcggccag
     6661 ggctacctgt tcgaattgcg ctgatgaaaa aattactgcg tctttttttc ccgctctcgc
     6721 tgcgggtacg ttttctgttg gcaacggcag cggtagtact ggtgctttcg cttgcctacg
     6781 gaatggtcgc gctgatcggt tatagcgtca gtttcgataa aactacgttt cggctgttac
     6841 gtggcgagag caatctgttc tatacccttg cgcagtggga aaacaataag ttgcatgtcg
     6901 agttacccga aaatatcgac aagcaaagcc ccaccatgac gctaatttat gatgagaacg
     6961 ggcagctttt atgggcgcaa cgtgacgtgc cctggctgat gaaaatgatc cagcctgact
     7021 ggctgaaatc gaatggtttt catgaaattg aagcggatgt taacgatacc agcctcttgc
     7081 tgagtggaga tcattcgata cagcaacagt tgcaggaagt gcgggaagat gatgacgacg
     7141 cggagatgac ccactcggtg gcggtaaacg tctacccggc aacatcgcgg atgccaaaat
     7201 taaccattgt ggtggtggat accattccgg tggagctaaa aagttcctat atggtctgga
     7261 gctggtttat ctatgtgctc tcagccaatc tgctgttagt gatcccgctg ctgtgggtcg
     7321 ccgcctggtg gagtttacgc cccatcgaag ccctggcaaa agaagtccgc gaactggaag
     7381 aacataaccg cgaattgctc aatccagcca caacgcgaga actgaccagt ctggtacgaa
     7441 acctgaaccg attgttaaaa agtgaacgcg aacgttacga caaataccgt acgacgctca
     7501 ccgacctgac ccatagtctg aaaacgccac tggcggtgct gcaaagtacg ctgcgttctc
     7561 tgcgtagtga aaagatgagc gtcagtgatg ctgagccggt aatgctggag caaatcagcc
     7621 gcatttcaca gcaaattggc tactacctgc atcgtgccag tatgcgcggc gggacattgc
     7681 tcagccgcga gctgcatccg gtcgccccac tgctggacaa tctcacctca gcgctgaaca
     7741 aagtgtatca acgcaaaggg gtcaatatct ctctcgatat ttcgccagag atcagctttg
     7801 tcggtgagca gaacgatttt gtcgaggtga tgggcaacgt gctggataat gcctgtaaat
     7861 attgcctcga gtttgtcgaa atttctgcaa ggcaaaccga cgagcatctc tatattgtgg
     7921 tcgaggatga tggccccggt attccattaa gcaagcgaga ggtcattttc gaccgtggtc
     7981 aacgggttga tactttacgc cctgggcaag gtgtagggct ggcggtagcc cgcgaaatca
     8041 ccgagcaata tgagggtaaa atcgtcgccg gagagagcat gctgggcggt gcgcggatgg
     8101 aggtgatttt tggtcgccag cattctgcgc cgaaagatga ataaatatgt ccttttacca
     8161 ctacatcaag tcattgattc gatcaaacaa ttttcattga aaaagtgcca aaaatcgcaa
     8221 attgactaca ccattaacta caccgttcgg tgcactgtat gaaacaacgt ggaacaaata
     8281 gacacaagaa atatacaggc gggtaatctt tccaggggga agcgcaccaa ttcatgaggg
     8341 gcgttaatgt cgatatgggg atccccataa cggggctacc ggattttttt tccggttaac
     8401 ctttaatcag gctggtgggt tttacatacc cttgatcacc gtaatgatga tccagatgtg
     8461 caccttttcc accaatggcg gaaaagctcc aaccgtaacg atgatcgcta tggttgcctc
     8521 gatggtctgc gtggtcatta tgaccgttca gccagtgacc ttaatcagaa tttttcgcta
     8581 aaaaatgacg ttggccacgt catccggaaa gcacaaaatc acgtcgattt ttaatgaacg
     8641 gttaaaccgt ctttttgtca cggtgtgtca ctggttgtca ctctccgtca cgattgaggt
     8701 ggtggtcatt ctgcccgacc gcgcatcttt gcgctatcgg gaatatcaaa cagttacctc
     8761 gccgacttcc tcaaattgag gtttccgaaa atatcagagc gggttagcga ttaagctgac
     8821 gagtaattaa actgcgaaaa tttcgtagtt tctgaatata ttccggcctg ctggttaaca
     8881 gtcctcagat gtgagggatg tagccagcgc aattttgcgc tttgctttaa tctcagtgag
     8941 ttaccgccgt aaccgctccg gcttcttcca gtggtacgtg attttctcct tctcccgata
     9001 cagttcaacg cggcgattgt aggccagcat ttccagaacg cggatccgta tgtcgcgcat
     9061 atccgcatca ttaagctgga taccatcacg gcgcatcacc tcagcaacaa cacgcgcata
     9121 attttcggct gtcacgctgt ccggctgcgt ggtctgttcg tcagcctgct ggctgattcc
     9181 agagacgcgg cggattaatc gcagtatttc ggcttctgtc atgctgctga cctcattaca
     9241 ccccgctaaa ttcttccagt ttctggcggt ggctgtcgct tatatcaaaa gcaaaatcct
     9301 catgctctgc ctggaatgta ccaaacgcca tcagcgccgc tacgctcggg tctatcttgt
     9361 tcggtgattt tttcttgttc ggcttgatat tggcgttcgc gtcactctgc atcacgacgt
     9421 tactcatcga ccaggacaac accggatcgc cacgatgcac aatcaccctg cggttaacaa
     9481 aaacttcgaa cgatttcgcc gccggactga atctgaggta ggtttgcggg aacggctcca
     9541 cctcaaaacc agccccctgt aattgcgtcc tgagatgcgt ggcgttccac gtatcaaagc
     9601 ccaccagcct gatattaaat ttctccgcat cctgcatgat gtcatctctg atccggtcgt
     9661 aatcaatgca gtcacctggc gttgtgcgta tccagcccgc tttagcccac tggcgataga
     9721 cagcgcggtt tttattggcg gggttctgta gctggaactc cggcagataa tgacgggaaa
     9781 tcagcataat ggttttaccg accggaaagg catagcatac gctggaaata tcgctggttg
     9841 atgataagtc cagccccgcg taacactcct gaccgtgtaa atcttcctcc gtgaacgttc
     9901 cggcacactc agcccatgca ccattaccca tccacggggt agtcccctga caccagatat
     9961 taaatcgctt tgtcatcatc tccacccact gcgacggaat accccgcgct ttctggatgg
    10021 ttgaggccag tttttcacga tccacggaaa catcaatatt agggttcgcc tttatccaca
    10081 tcgccggatc atcaacctcg ttttcgtcgt ccagttcgta aatcagtaca aaaattgaat
    10141 cgttaacctc ttcgccgtcc aggatctggc agcaataatc atagtgctgt ttacaggctg
    10201 aaacgacgtt actgcccgat gtggtaatgg caaataacag cccttcggga cgtgcgccca
    10261 tccccagctc aagcgcggaa taaacgccgt tatcggggtg taggtggtat tcgtccacga
    10321 tggaaagact ggggtttgtc ccctcgatgg ttgccgcttt tgctgccagc ggctttaaca
    10381 ggctgttgct tttcgggtgt atcaccttat gcgcctgaat attcacccgc ctgcgtaacg
    10441 gtcgggataa aaggcacatc tgacgcgcat tatcaaacac gatccgcgcc tggtcacggc
    10501 tcaccgctgc ggtgtagata tcctgctgac cgttctccat aatcagaaac cagttagcca
    10561 gaatcgcggc ggtcgtggat ttcgcatttt tgcgcggcac ttcgataaag gcgctcgtgt
    10621 atttgcgccg tccggtggcc ttaaccttaa agccgaggat gcacgcaaag gcgaactgct
    10681 gccacggctc cagctcaatg ggtctgccac gcatcggtcc ttttacgtgc gggcacaccc
    10741 tggaaaaggc aataaaccgc tccacaacct cacgatcgaa cgtgtaaagg gggcttttaa
    10801 ggtccgaaaa gtaccgttta acagcctgtt ttaaccgttt acaggccgga attttgcccg
    10861 tttttacgtc ttctgcgtac ttattccagg cggtcaagct cgtcctcttc ttctgtttcc
    10921 ggtggatttt tacggcggct tatcgggtca aaaccgagca aggaggccat ttttatcatc
    10981 actctttcag cgtcggattt tgcgcttaat gcggggtttc tgctctcgcc gccctgactg
    11041 ttaacaatgc tgaacccgcg cgccgcaagg tctgcaacgg ctttccggta aatggagtag
    11101 ttgacgctat acagttccag attgctccag tcggcggggg tcaggtctcc ccgttccgca
    11161 agctgccgcg atttttcccg ccactgcttc acggcgatat catccaggta ggcggggtcg
    11221 ggtttgtcct gaaaacgccc cccaccccgt catacagcct catcagcgat tgcggaaaca
    11281 ttccataacc tcgcggtcac ggtcggttaa tcgcttcgct gtgatgcgtt ctgcgcgtcc
    11341 tgacgcttta tctttatgcc ctgtttcctg tgtcttccat gcgtcacgct gccttataag
    11401 tccacggata aggcggtttt gttcccgctc attcatcagc gtcatacatc cagttattgc
    11461 ggttagcagc ccgttcttcc tcttcacgaa atccacctgc ggcacgcttg ctttttgttg
    11521 ccggatcaag ccatttcgtt ttctggttat gacacgcctg gcacaatggt tgatggttcc
    11581 attcgggcca gaagagaaca tcatcaccgc cattaatcgg gataatgtga tccaccacca
    11641 cggcgggcgt atatatcccc ttctcaaggc atcgcacgca taacgggttt ttactcagat
    11701 acatggcgcg gtatttgtcc cactgtctgg aatatccacg cgcgcggcgg tgtcctcgtc
    11761 tggcatcctc tgcacgccat gccgcccgcc tgtgctcctc acacttgccg gatttaacgc
    11821 gcctgttaca gcctggttct gtgcatcgtc ttaatggttg ccacggcatc agtacccccc
    11881 cacatcacgg taagccgtcc agagtgcgcc tatcgtcatg ggtacgcgtg tttttgcgtt
    11941 atccgcgaca atctggcgat tttcatacag gtgagcgata aacatcatgc agccaatctt
    12001 tatggctggc gtgaacgcca gcccgtcatc aaagcgcctg cctatgtgta tctggcacgc
    12061 ctcaagcgat gcggcaatgt atccgctgat taactggtct tcatcgtcgc catcgatgcg
    12121 gcaatggagt ttcacttctt ccagggtgat aagttcttct gtcatttttc cgcgccctca
    12181 cgacaaagaa tttcaaggcg tgtcctggcg gcatccggca gcggctgccc gatgatattc
    12241 agtacacgcc ccgccagcgg ccccgtactg acctttatcc ggctggtggc gttgatgtcc
    12301 ttccggtacc gtatccagat ccttacggta ccaatcgcca gctctgcgcc cgatgaaatg
    12361 gcctccttgc tgctgaccat gttcacgctt gcccagagtg tgtgaccgtc ctcccacgtt
    12421 tcgagtattt cgccggttat atccctggtc tgtttcaggg tctgaatcgt tatcctgtca
    12481 cgcagtcgcc ctatgttcat tccggttttt ctccctcgct gatttttact tcctgtttcc
    12541 atgcctggct gaactcatca ccaccatcac gcggtgacag cccttcacgt tcgcgggctt
    12601 cgttcggaca cataacgtcg gattttatgc cgcgctcata ggtggcaaag cgttcgccag
    12661 gtgtggcccg taacaggtcc gcgctgtcaa actccacctg ataccggata ccaggtacag
    12721 gcgaggccac cagcaaggcg ttttttatct gctgctcaaa gttcgccagc cacgggcgca
    12781 tcgtcatggt gagaaatgcg cggcttgcct cgctgaagtt gctgtaggtg ctgttgctgt
    12841 attcctgcaa aaaaatcggc gagacgttga acattcgggc gatgtcttca atggtgaagc
    12901 gacgggaggc cagccattcg gcatcctgat tactcatgcc cagctgctga tagctcatcc
    12961 ccccttcaag gatgggcgtt tttccggcgt ttctggcccc tttgtagcgt tccagtgcgg
    13021 ctaatgcctg tttgcccttc acgccgtcca gccattcgcc tgacgtgata acccctgccg
    13081 ccatcatgcc atcacgcatc acgctcgcgc cgtggcgttg ttgggccagc ccaagcccca
    13141 gcgattcgcg gcagatggtt acaggtgagc gccccataaa accgtcatcc gtggagtaac
    13201 gaaggtggag aacctcccac ggtaaatagt tgcggatgtt tccggtgtag gcgtcagtga
    13261 tgcagtagcg ccagttatga tcacctgtct gctccacgtt caccgactgc ggcgggtaag
    13321 gatgtaaagc cgccggaaaa ccatcacgcc cccactgaat aaccgcataa gcattaccac
    13381 ttaacaggca gtagcggatc atcattcgct taaactggta gggtgtctgc cacgcgttcg
    13441 ggcgctcgtt gaggatgtga tcgaccggat gagaatcaag ccattcgcgg gcctccttcc
    13501 ccttctcatt gcgtaccagg tacaggtaac acggcatggt agccaccgcg tcagagatga
    13561 ccgtgacggc gttcatgacg gcggggagtg attccgctgt cccagatgat acgtactcac
    13621 ccgcccccgt gttcgatgtg ccggccagcg ccataaattc atcaagcgtc atgctgcgct
    13681 gctctttttt tcgtctgaaa ggccacatat cacacccccg ctaaatccgc ccaccagcgg
    13741 cgattatccg cacgcggcat tttttcgggg tgctgctcat acagggaacg gcgggccagc
    13801 tccacgccgg aatcggggta agccggtacc gatgtaacgg taatttcgta cagttccgcc
    13861 accagcacgg tgcgcacgca tggatctgtt gtggtatccc atacatcctt acgggagcga
    13921 aagccaaagc tcatccccga gatgtcccca cgcttaacca gttcgataac gtcgcgccct
    13981 gtactggtat ccggtggggt cagttcaaag cgtaaccctg tttcgtcctc ttccagtttc
    14041 agcgtgccgg aacgggtgcg ccccagtaac atgctgtggt catgctcata caggccgcga
    14101 acgtcattac ccgccgcaag ccattcagta aacgcccccc gctggaattt ttcgtaaaac
    14161 tcaccccata acagttctga aagcttatcc cagcgaacaa cgtagcccgt cagcgtaccg
    14221 gctccgctgg tggtgatttc cgatgaccgg atttccatac tcttcataat tttttcaccc
    14281 ataaaacact aaaggggctt tttagcccct tctgtatgct gttaatcgtc gtcttccggc
    14341 agtgccagaa tcttgatcgc gttcgaatcc accacgccac cgcctaaata tttttgcgtg
    14401 aaaattttga tgaatcctgg ttctgtcagg ttgtccggtc tggtgcgaac acctgtttcg
    14461 tgatcaacaa tgtagtaacc gcgtttgaag tcacccaggg caataacgtt atcaggcata
    14521 aactccagat attcgaccgg aaggcccagc aacgtatcag gatcacccgc ctgtaaacgg
    14581 tcgcgccaga tataatcacc gttcgcgttc ttcaccttct gaagttttgc cgccgtcgtg
    14641 gagttaacca ctcagaccgc gtttttgcgg tattttttac gtaatgcaaa tttcaggtcg
    14701 atcagcgggt ccgcagatgt ccacgccaga gattcggaag gtttaattac ctgcaacgta
    14761 ccaaaatcac gctctttgtc gttcttctct gcacggggta cggataaaaa accttttgct
    14821 tttttgtcac cgtcgcccac aaccagatcg ctttcttcgg tttccgtgaa ggtgtcgcca
    14881 atctcacccg tcagccatga aaggatgtcc acatcggaaa aatccacgat ttcctgtgtg
    14941 gtgcgcgggt acgcatagac cggatacagc ttaatgctca cctcgttaat ctgcggggtg
    15001 ctggtctgtt cgcgtgcctt accctcttca ccgtggttaa cggtcgcacc gccagcggaa
    15061 acaagctgct taaactcgtt gctgctgatt ttcttcacgg tacagatgcg acgcatggtg
    15121 gattcatccg ccagcattcg catgatttcg gtgttcagtt cagggataac ggtataacca
    15181 ccatcagcgg gaacgcctgt acttaatgcg cgggtttcac cggtcagaat gtagttacgt
    15241 agttctgcgg ggtcagtggt ctggctgctt ttacctggct tgctgcgctc ttcgtctgca
    15301 atggcttcaa ggcgggaaat gtctttatcg agggattcag ctttagcgcg taattcgtca
    15361 aattttgcgc cctcagcatc gttaagactg cggttttctt tttcagcgtt ctccagcatg
    15421 tcgcgcatct gatttttaat ggcggttttc tgctggcgta attcgattat tctctgcata
    15481 aaaaaagtcc tggggttaag taaggaactc caggacgcgg caaacactca accgtttttc
    15541 ataaggaaat cagtaatcgc accgatcgtt ttcccgcctg gtaatgaata ttggcgagca
    15601 cattaacagg cgggaaagtg gccccagcgt cctggcacca cgggcgagaa taatcatgat
    15661 tcagttcggg taaaatatgc cgatccgctc agtgaacaac gtggaacaac cacgaacaaa
    15721 taatttacaa aaaataacaa aaagccggat tgctccggct gtatgtgctg tatgggtgct
    15781 cagtctctga tccctttcag aaattctatc aatgcatctg tctgttcagg atttactgcc
    15841 agcatttcac cggatagcgc gcaacgaaca aaaccatgat gatccttttc aatcagcgcc
    15901 cccgtttcca gaaatgcgcg gtaatcagtg atattcatcg tctccatgtt gtcagcatgg
    15961 tatttatcac gctgtttaag tatctcatca aatttcatcg gcattgcgtt ttcctctgtt
    16021 gtgtctgttt gtttcagata gtaactatgc cggaccgtga cgaaaagcca gtagtgctcc
    16081 ataccgtttc aaatgacgca aaaaaagccg gattgctccg gctgcgtggt gtggttctga
    16141 tattcctact ctgctaattt gctcagcccc atgtctgcgt aagtgcgatt tactgcattt
    16201 ctcaggtctg cgtagttctc cgttggcggc tccggtgtgc ccttgctgct ctgttttacc
    16261 agcgggtcgc ccaccagctg cccgtaagct gcaatttgtg ctgtcatatt gccacctcag
    16321 tgaagcgatc cggcattgtt ctttttcatc tcttccatca ttttttcagt gagcattacg
    16381 cactgaaacc ccatgttttt gatgtctacg ggtttcatgc ctttgtcagt ggatatcaca
    16441 ggcagaatat cctgatacac atcgcggtca gggtaactaa gcacacaaag ctcccatgat
    16501 gccacgggtg aggatccttc gtctgtttca acccaccagc ctggtgtagc tgcgcttatt
    16561 tgcagaatcg tgatccgtcc gttgtgcttg taaaattttt caccaaattt tggttcgctc
    16621 atgattccac ctctccggtt ttaacgttga ttgttgttac ctgttccgct tcggcaatct
    16681 cccgctctgt cagcgtggca aaatttgccg ccgtcgtggt catgaatgcg cttatcagtt
    16741 cgggatgtgc tttcgcgtat ccttcccccg cgtggcggtc tatcgttctg attgccacct
    16801 ttaaagcgtg ctctgtcatg tctaacgcgc gatatttcgg ttctgttctg tctctgcgtt
    16861 ttttgaatga tttattaaat ttccctgaag tgtgcatatt tatttttacc ccctcgttta
    16921 aaaagttttg agttgtgcct ccccttgtct accttatcta ccttagtggc cctcatgcca
    16981 gtaatggcgc tgctttcagc ggggtagagt gcttttatcc actatctacc ccgtgtctac
    17041 ctccctgtct gattcaggta aaatcaggta gagagggtag atagtgggta gacagtaaaa
    17101 aaaggctatc tacctaactt aatgcactga attaaatgta tttttcttta ctcaggtaga
    17161 cagggtagac agcaattaca aaaaattata aaaacgcgtc gcactcgtct gttgttattg
    17221 cgttagtctg cgttactccc ttaactttcc gcgtaatata ttcatgtccg taaactttcg
    17281 cggctggctt catagccttg ccaaagtcat ttacgtttag cggtttgctc ctgcctgcgt
    17341 acgccataaa cgccagatag acgcggtaaa ggctgtttct ggtcgtgtac ttcactgaat
    17401 cgccaccgcc gcccatcatc aggccgcgtg cttcctccag aaaattcagg aactggcaaa
    17461 actcaataac cggatccgtc tgttgcttta ttgccagagc ttcatcaccg tcacgctgtt
    17521 ccagtagtaa agcccgtgct ttctcagagt cggtaaagtt cgccagcaat cggcggataa
    17581 tgacagggat ttcagccgca atcttttccg gtagctccct gtctttttcg gcctcactaa
    17641 cgatattgtc gaaacggaaa atcacgcgac gacgtgccac acctccggcc cgttcggtga
    17701 atatcatcgg gttattgttg gtcgccagca ccaccgccct gattacagcc gtgaaacgct
    17761 tttcgtattt cgggttaatt tccacggggt caccgcccgt gattttcttg atgcccgttc
    17821 cttcgcctgt atatttcggc tggtcagcca ggacgataag acgactcccg acaacctgcg
    17881 cacgtccacc agcatcatca agcgatgtca tttcagcgct taccgtgttc tgtttccctg
    17941 ccagaaggct ggctatgtgt gtgaatgtac ttttaccgct cccgccgtct ccggtggcct
    18001 caataaacat ctgccagtcg taccggttcg ccataatcat gtacagcgcg gcacatatac
    18061 gcatcatctt gcgctggtct tttccggctg cgtgctcaag ccatttatga aagtttagcg
    18121 cgttatcgcg gatgttctcc cctggtgctg gtggcgtgta ctcaatgccg ttgtgcgtgg
    18181 tgatccagtt ctccggcgtg tgcggggaaa attcccccgt tttcaggtca agcgcaccat
    18241 tggcgaacgg cagcaaatcg ccggacggct cgcccattgg ttcggcaata acttttaacg
    18301 cttccacggc gttattgatt acgcgcttgc tgaaagtggc cctgtgctct gaatagatca
    18361 ccaccatttc gcggctaagt tccattgtgc tgaccggaca ccataccccg ccgcgccata
    18421 cgtgaacgat ttcactttcc tgatgcacac aaacgccatc aaagcgctcg gcaagcagct
    18481 gcgcgcgctc actgtctgcc atttgtgcca gttgcgtatt tttctgctcc ggcatggtaa
    18541 gccctgcggc aatattttca cgctcagcat tcagatagcg ccgccagttt tcacacttct
    18601 ggacgtgcat tccttcgggg taaaaatttg cgtcctgaat atctgccgcc gccagcttct
    18661 gaccaatctt tttggtctcc accaaatcca gttctccggc ctggtacagc cttacgcgtt
    18721 tttttccatc cggaacaatt ttcagtgcat caagttcggc aagctgattt ggcccaagcc
    18781 acacaggcgg cacattatcg ccggatgcgg ggccatcctg ctcctgccat tgtttcgcgt
    18841 gcgaccaggc atcactaccc gcaaagataa ttacctctgt gtctttgtgt tttattccgc
    18901 gtggctgttt ttttacgttc ggtgccagtt tcatttttta cccctgaata cgttaagcat
    18961 cttttttatt tcctgaatat tggcgcgtgc tttctccctg ctggttggtt tactgcgggg
    19021 cgctgcctgt accagcgtga aatcacgccg gaattgataa acaggcatca cgcaatcata
    19081 ttcgtaacct tcacggcggt aggtgatgcg ccgttctgcc acgcctttaa tcgttaccat
    19141 gccgccgtac tggtcgcggt aaatatcacc gcgcgtaaat ttagggtgag tgttgccact
    19201 ggcagttaag ccagaatatt taagtttcat tatttttatt ctccggtgtg ttgttcttta
    19261 tatctgtcgt gcaatagatc tatttcttgc agttccatta ttacaggctc aagaagcgtt
    19321 attaatgccg tggcaattct tgatttttgt ttgtcgcgtt cattgtcgcc aagtgtttca
    19381 agccatatgc gcaatatttc cagcatgttt tcactgtgag aaagtgcaag aaatgcgcgg
    19441 tctattgttt cgtggtaaat atcacgcata ttaatccccg tccgtcgttt ttcttaaaat
    19501 aacctctgtc acgaaatcag cataatcggc ggcgatattc agtacatcaa gcccgttgat
    19561 ttatattctc tcgtggaaag taagaaaaaa gccgctctaa taagttctgg cattgacgaa
    19621 agcgcatcag ccgcatcatc aggaacgccg gaaaattcct gtttcaggga attaaaacga
    19681 tcatcacgca taaccccccc cattttcaca atcggcaaca agaatatttt tagcgtcatt
    19741 cagtgaccgc gttgcgatgt atcggataca ttccagggcg aactgtgtcc ggtctttctg
    19801 ttcttctccc tgcgcaagct ctgccgtgca ttcaatatca ataagcgcgt gcatcagcgt
    19861 agtgagtgcg gcggctgctg cgttcggtgt ggttttattg tgcataggtt tccccctgac
    19921 gaatacgggc ggcgaataca gcaacacaac cggacgggca acggctacgc gcttcgcgtt
    19981 ccgtccaagc ggttacgtgg atgatttggg attctccggc actcagtgcc agaaaacgcc
    20041 acacaaaggc cgtttgtgtg tgtacaaggt gtggtatatg atttacggca accataacgg
    20101 ctcctagttt acgttgttgg ttagaagccc cgttactgct cctacagtgc ggggctttgc
    20161 tttttgtgtt gattgtgtgt aatgtgtcac tacacataaa tacattacct gaggtgtaat
    20221 taacgtgtca acacacaaaa acgaaaggcg tggtaatcct ccgttccagt tccgtttaga
    20281 tcctgactta cgggaaaaga tggaaaaggc acaacaacag gacggcgatg attctcttgc
    20341 tgcctggata aagcgaataa tccgcaaaga attacagcaa cgcggcatag aacccaaagg
    20401 ataaaaacta tcagcgccgt ggtgtgaggt actacggcgc attgctttac aggcgcacac
    20461 aatgaccaac gaagaatcaa ccgaaaaaac atcacctgca cgaaaaagac gacgcagaaa
    20521 gaaagagcat gaatcagaac gattcgcgcc gtgttctttt gcccttgaga agtaccttaa
    20581 agaatactca tcaaaggaga gaacttcgca gttatggcaa cgcactgaac cagactgatt
    20641 gcattgccca ccagcctgat agcggctatc atccccgtgc ttatgtttgg gatcacacac
    20701 acaaacggcg cagcgggtta tctgttcaga aaggcggctc catttcgggg ccgctttttt
    20761 tatgcctgaa aaaccccaat tttgtggttt tccagtttca ccagggcgaa cgaatccccg
    20821 cccacgttct ggcgtatatt caatcttcat ggttatagct ctgtgttcag atgattgatg
    20881 tgtggcggct gcgtgccgcc agcgtgatta atgaactgcc ttgcagctat ccttccaggc
    20941 cagaacctcg gataaagacc agccaacgga acgaccgcca agtttacgac gtgatgggaa
    21001 ttgtccggcc ttttccaggc ggtagcggca tgagcggcta aggcctgtta gcttttcgca
    21061 ttctttttca cgtataaacc gatcagtgct taacactatt gccccctttc gtttcttaaa
    21121 gagttatttc gtgttctatt gcgttgggat gtgtctgatt gtgtcaggat gattcagagt
    21181 tggcaaatgt tgaggccgta tggtttacag aaagaggaat aatcaggata aaatccttta
    21241 aattcatgtt aatacaaagg cataaaatat tgtttcatgc cttttttctc gctatttaaa
    21301 gagtgattcg ctagtgtata aaaaatcagt aacacattaa aaatcagcta cttataaatc
    21361 tgtacacttt ttcacctctt gttcgtagtt gttccgcatt gttgctcatt gttgcacgtt
    21421 gtatctgttc gcatatccag tatgcgcata ctgaaaaaac acgaaaaaaa ttattttctt
    21481 ctggctactg gtagcgtggt tacgttttca tgtgttcccg ccagtatccc taaccgctcc
    21541 gtccacatat ccagcgcatt gcgtttagca tccagataac gggaatgatt ataaactcgc
    21601 tgcattcctg gcatctggtg gcctgtaagc tgctccacga cgtgaggatc cacgcctaaa
    21661 tcgttcagca tcgttgtaaa ggtgcgccgg atgtcatgta gtgaccagtg agggtgattt
    21721 agcctcctgt gcgctaatct gccgtactgc gacacgcttg tttcctgttt cacttccccc
    21781 agcaataagc ccgtgtgcct gttctgctcc accagctgcg tgacgaacgg cagtattgct
    21841 tccggtatgg gccggaatat tgccaccttc gttttgctgt gttctttcgg cacggtccag
    21901 agcatttcgg taaaatccca ctcgctgatc tccgataacc tcagttctac cgtccggcat
    21961 ccgaacacaa tcaggaggcg gattaacgcg atgtagtagg gggagaatat ttttttgtcc
    22021 agtgcctgca ataattcgcc cagttctttg gtgcttaaga cacgctcgct tatatccggt
    22081 tttttcccaa cgtccgccac actcatatca tcaagaacgt tgctgattgc atagcgccgc
    22141 ctccggcaga acttaagcgc ctgtttgcac gtctgtagca agaatccggc agtaacaggc
    22201 gttcgctttg ccacctggtc aaaacaggcc agccagtgcc gtagctcgca tttatccagc
    22261 ggcatagcac caatgtgctg tattacgtga ttattaaggc gctttttcag ggcggcataa
    22321 tccacgcggt tttcctttgc gtacgactca agccagtagg tgagcgcatc gccaaccgtt
    22381 accggcttta acgcttcctg tacggtgtaa ttaagctcat gacgtggatt tttcccctct
    22441 gccagccatg cgcgacactg ggcggctttt tccctggctg atttcaggct cagatcagga
    22501 taatttccca gcttaatgcg ttccggtggt gccccccttc ccgttccggc cctgtaagtg
    22561 aaataccagg ttaaaaggcc actggttgaa tgcctgacgc tcaggtttcc accgtcatta
    22621 agaaaggctg ttttttgggc gggtgtgccg ttgattttcc tcagctgtgt atcgctcagt
    22681 ttgttaagtg ctctgctcat aattttgatt ccggtcatca caattaacta caccattaac
    22741 tacaccgatc gttgcacaaa gggctacaac gtgaaacaag ctggaacaag gaaaatcaca
    22801 aatctgttta taatcaaaaa cataatgaac aatctgaaac attatgaaac ggcaaaaaac
    22861 actaattgat aaaatatgtc catacttcac gcattacgtt aagcatccgt tataatcggt
    22921 tgcagatacc agcctgtgga tgcttaacat ggaataccaa ctcactctta actggcccga
    22981 ttttcttgaa cgtcactggc agaaacgccc ggtggtgtta aaacgcggct ttaataattt
    23041 tattgacccg atctctccag acgagttggc gggtctggcg atggaaagcg aagttgacag
    23101 tcgactggtc agtcaccagg atggcaaatg gcaggtcagc cacggcccgt tcgaaagcta
    23161 cgatcatctc ggtgaaacca actggtcatt actggtacag gcagtaaacc actggcatga
    23221 gccgaccgcc gcgctgatgc gaccgttccg tgaactaccg gactggcgta ttgatgatct
    23281 gatgatttct ttttctgtac ctggcggcgg cgtcggcccg catctcgatc agtacgacgt
    23341 gtttatcatt cagggtaccg gacgtcgtcg ctggcgagtg ggcgaaaagc tgcaaatgaa
    23401 acagcactgc ccacatccgg atctgttaca ggtcgatccg ttcgaagcca tcatcgatga
    23461 agagctggag cctggtgata ttctttatat tccgccagga ttcccgcatg aaggctacgc
    23521 gctggaaaat gcgatgaact attccgtggg ttttcgcgcg ccaaatacgc gggaattaat
    23581 tagcggattt gccgattatg tgctgcaacg tgaactgggc ggcaactact acagcgatcc
    23641 ggatgttcca cctcgtgctc atcctgcgga tgttctgccg caagagatgg ataaactgcg
    23701 tgagatgatg ctcgaattga tcaaccagcc ggaacacttt aagcaatggt ttggcgagtt
    23761 tatatcccag tcacgtcatg aactggatat cgcgccgccg gaaccgcctt atcagccaga
    23821 tgaaatctac gatgcgctga aacaaggtga tgtgctggtg cgcctgggtg gtctgcgcgt
    23881 attgcgcatt ggcgacgacg tgtatgccaa tggtgagaag atcgattccc cgcaccgtcc
    23941 ggcactggat gcactcgcca gcaacattgc gctgactgcg gagaattttg gcgatgcgct
    24001 ggaagatccg tcattcctcg cgatgctcgc ggcgctggtc aatagcgggt attggttctt
    24061 cgaagggtaa gtttggattt aagccggatg cagcatcaca tgccgcatcc cttttcgctt
    24121 acttccgttg cgccgttaac tcggcaatac ggacgatcac ctgcaccgct ttttccatac
    24181 cttccagaat cacaaactca tgcttaccat gatagttgta accgccagtg aacaggttcg
    24241 ggcaaggtaa tcccataaac gacaactgcg cgccgtcggt accgccgcgg atcggtttca
    24301 gttccggttc aatatcgcag tcacgcatcg cctgctgggc gatatcgaga atatgcggat
    24361 gctcaaccac tttctcgcgc atattgtagt aactgtcttc aatcaccaat tcaatgtagc
    24421 aatcaggatg taaccctttg cccacttttt tggcgatctc catcatttta cgtttacgcg
    24481 cttcaaactg tttacggtcg aaatcacgga tgatgtagtg catatcggcc cgttcaacgg
    24541 tgcctttcat gctcgccagg tgatagaaac cttcatagcc ttctgtcatt tccgggcttt
    24601 catccgccgg aacttccgca tgaatacgtg ccgccagcga cagcgcattt accatcactc
    24661 ctttcgccgt gcccggatga acattgttac cgacaatttt tatattgacc gatgcggcgt
    24721 tgaagttttc aaactccagt tcgcctacgc caccaccgtc aacagtgtaa gcccagcggg
    24781 catcgaaggc atcaacatca aaatgtttcg cccctttgcc cacttcttca tccggggtaa
    24841 aggcgacgcg aatatcacca tgcggaatgt ttttctgttg caataccgcc agcgcggtca
    24901 tgatttctgc aatacctgct ttgtcatcag cacctaacaa ggttttacca tcggtggtaa
    24961 tcaacgtctg acccagtagc tgatgcagca ccgggaacat aaccggtgat aaaacttcat
    25021 cgccgatacc cagcgcaata tcgccaccgc gatagttttc aacaatttgc ggattaacat
    25081 ttttgccgct gcaatccggt gaggtatcca catgagaaat aaagccaatc gccgggatat
    25141 cgccagggac gttagccggt aacgtcgcca tcaaagtgcc cttctcactt aaggtcacat
    25201 tgataagccc catttcttcg agctgctctt tcagcagatg caataacttc cattggcctt
    25261 ccgtgctggg aacctgtctc acccctgctt ttgattgggt atccagagac acgtagttca
    25321 aaaatcgctc aagtagttta tccatgtagt caccctcact ttttgtgaca acattattaa
    25381 gaagcaagaa aagacaaata ttgcgtcagg tcacttttac ccctgcaagc gggaatattt
    25441 atcagcatta ccttaatgaa tataaagcta agccagtaat tcacaacaaa gattggcgat
    25501 tcaagcggtt tgccgtagaa ttaccgccct cattaggagt caccaaggtg gttaaccaca
    25561 aaccccgcat cggtaagcca tccgttgcgt ttacatggga cagagtaaaa aattgaataa
    25621 acaaccgagt tcgctttcac cgctggtgca attggcggga attcgcaaat gctttgatgg
    25681 taaagaggtc attccccagc tggatctgac tatcaacaat ggcgagttcc tcacgctgct
    25741 tggcccttct ggctgcggta aaacaaccgt tcttcgcctg attgcaggtc tggaaactgt
    25801 tgattccgga cgcatcatgc tggataacga ggacatcacc cacgttccgg cggaaaaccg
    25861 ctatgtgaac actgttttcc aaagctatgc acttttcccc cacatgaccg tgttcgaaaa
    25921 tgtggccttt gggttgcgca tgcaaaaaac ccccgctgct gaaattacgc cccgcgtgat
    25981 ggaagccctg cggatggtgc agctggaaac cttcgctcaa cgtaaaccgc atcagctctc
    26041 tggtggtcaa cagcaacgcg tcgccattgc tcgagcggtg gttaacaagc ctcgtctgtt
    26101 gttgctggat gagtcgctct cagcgctgga ttacaaactg cgtaagcaaa tgcagaacga
    26161 gctgaaagcg ttacagcgta agcttggcat tacattcgtt ttcgtgactc acgaccagga
    26221 agaagcactc accatgtcgg acaggattgt ggtgatgcgc gatggtcgca ttgagcaaga
    26281 cggcacgccg cgtgaaatct acgaagagcc gaaaaacctg tttgttgccg ggttcattgg
    26341 cgaaattaat atgtttaacg ccacggtgat cgagcgactc gacgagcagc gcgtacgcgc
    26401 caacgttgaa ggccgcgaat gtaatatcta cgttaacttc gccgttgaac cggggcaaaa
    26461 actgcatgtt ctgctgcgcc cggaagactt acgtgttgaa gagattaacg acgacaacca
    26521 cgctgaaggg ctgattggtt acgttcgtga gcgtaactac aaaggcatga cgctggagtc
    26581 ggttgttgaa ctggaaaatg gcaagatggt gatggtcagc gaattcttca atgaagacga
    26641 tcctgacttt gaccactctc tcgaccaaaa aatggccatt aattgggtag aaagctggga
    26701 ggtcgtactg gctgatgaag aacacaagta agttccagaa tgtagtgatt gtcactattg
    26761 tcggttggct tgtgttgttt gtctttctgc ccaacctgat gatcattggc accagctttt
    26821 tgacccgcga cgacgccagt ttcgtcaaaa tggtctttac actggataac tacacgcgtc
    26881 tgctcgatcc gctctatttt gaagtgctat tgcactcgct gaatatggcg ctgatcgcca
    26941 ccctcgcctg cctggtgctg ggctatccat ttgcctggtt tctggcgaag ctgccacaca
    27001 aggtgcgtcc gctgctgctg tttctgctga ttgttccgtt ctggaccaac tcattgattc
    27061 gtatctacgg gctgaaaatt ttcctcagca ccaaaggcta tctcaacgag tttttgctct
    27121 ggctgggcgt tatcgacaca ccaatccgta tcatgttcac gcccagtgcg gtgattatcg
    27181 gtctggttta cattctgctg ccgtttatgg tgatgccgct gtactccagt atcgaaaaac
    27241 tggataagcc gttgcttgag gcggcgcgcg atctcggtgc cagcaagtta cagactttta
    27301 tccgcatcat tattccactg acgatgccgg ggattattgc cggatgtctg ctggtgatgc
    27361 tgccagcgat gggcctgttc tatgtgtccg acctgatggg cggtgcgaaa aacctgctga
    27421 tcggtaacgt catcaaggta cagttcctca atattcgtga ctggccgttt ggtgcagcca
    27481 ccagcattac gctgactatc gtaatgggcc tgatgttgct ggtttactgg cgcgcttctc
    27541 gtttgctgaa taagaaggtg gaactcgaat gatcggtcga ctgcttcgcg gcggttttat
    27601 gaccgctatc tacgcgtacc tgtatatccc aatcattatt ttgattgtga actcctttaa
    27661 cagctcgcgc tttggcatca actggcaggg ttttaccacc aaatggtata gcctgctgat
    27721 gaacaacgac agcctgttac aggcagcgca gcattcacta acaatggcgg tgttttcggc
    27781 gacgtttgct acgcttatcg gttcactgac ggcagttgcg ctgtaccgtt atcgctttcg
    27841 cggtaagccg ttcgttagcg gaatgctgtt tgtggtaatg atgtcaccag atatcgtgat
    27901 ggcaatttcg ctgctggtgc tgtttatgct gctgggtatt cagcttggct tctggtcgct
    27961 gctgttctcg catatcacct tctgcctgcc atttgtggtg gtgacggtgt attcgcgcct
    28021 gaaaggtttt gacgtgcgga tgctggaagc ggcgaaagat ctcggtgcca gcgaatttac
    28081 cattctgcga aaaatcattc tgccactggc aatgccagcg gtggcggcgg gctgggtgtt
    28141 aagctttacc ctgtcgatgg acgatgtggt agtttcttca ttcgtcaccg gaccaagtta
    28201 tgaaattctg ccgttaaaaa tttattcgat ggtcaaagtc ggcgtatcgc cggaagttaa
    28261 cgcgctggca accatattac tggtgctgtc gctggtgatg gttattgcca gccagcttat
    28321 tgctcgtgat aaaacgaaag gtaacacagg ggacgttaaa tgaaaaaatg gtcacgccac
    28381 ctgctcgcgg cgggtgctct ggcactgggc atgagcgccg ctcacgccga tgacaacaac
    28441 acgctgtatt tctacaactg gaccgagtac gtgccgccag gactgcttga acagttcacc
    28501 aaagaaaccg gtattaaggt tatctattcg acttacgagt cgaacgaaac catgtacgcc
    28561 aagctgaaaa cgtacaaaga cggtgcctat gacctggtgg ttccttcaac ctattacgtc
    28621 gataaaatgc gtaaagaggg gatgatccag aagatcgaca agtcgaagtt aacgaacttc
    28681 agcaatctcg atccagacat gctcaacaag ccttttgacc cgaataacga ctactccatt
    28741 ccgtatatct ggggtgcgac ggcgattggt gttaacggtg atgcggtgga tccgaaatct
    28801 gtcaccagct gggccgatct gtggaagcca gagtacaaag gcagcctgct gttgaccgac
    28861 gatgcccgtg aagtgttcca gatggcgctg cgtaagctgg gctactccgg taacaccacc
    28921 gatccgaaag agattgaagc tgcatataac gagctgaaaa aactgatgcc aaacgtcgca
    28981 gcgtttaact ccgataaccc ggctaacccg tacatggaag gcgaagttaa cctcggcatg
    29041 atctggaacg gttctgcttt cgttgcacgc caggcgggta cgcctattga cgtggtgtgg
    29101 ccgaaagaag gcggcatatt ctggatggac agcctggcga tcccggcaaa tgccaaaaac
    29161 aaagaaggcg cgctgaaatt gatcaacttc ctgctgcgcc cggatgtggc aaaacaggtt
    29221 gctgaaacta tcggttatcc aacgccaaac cttgcggcgc gtaagctgtt aagtccagaa
    29281 gtggcaaacg ataagacgct ctacccggat gctgaaacca ttaaaaatgg cgaatggcag
    29341 aatgacgttg gcgcagccag cagcatttat gaagagtatt atcagaagct gaaagcagga
    29401 cgttaattca gcaagcgggc ggtggtatta ccgcccgttt tttagttagc aaggatgtgg
    29461 ctaatgagtc aggacagtaa agttttatcc cgctctttcg tcggtattgt ggtccgcttg
    29521 taggcctgat aagcgtagcg catcaggcaa tgcttcctgc tttataatcc ctccagcaac
    29581 ttttcaacaa attctggcac cacctggctt gccgggccgt aatatttctc ggcaaattca
    29641 ttaccaacct gactcggttc aagattcagt tcaacggtgt gagcaccatg tagtttggct
    29701 tcgtgtacaa acccagccgc cggatagaca tgcccggaag taccaatggc aataaagaca
    29761 tccgccatcg acaacgccat ataaatttca tccatgccga gtggcatttc gccaaaccaa
    29821 acgacgtgcg ggcgcaaggg tgccgggaac tggcaacaat ggcatttatc ttctggcgta
    29881 acgtctccgg tccagtcgag aacctgacca ctttgcgaac aacgaacttt cagcaattcc
    29941 ccatgcatat gaatgacatt ggtattacct gcgcgttcat gcaggttgtc tatattctgc
    30001 gttaccagca aaaagcgatc gccgagagcg tcttgcagtt tagccagcgc aagatgcgcg
    30061 gcgttaggct gaatttctgg ctgctgcagc tgtcgacgac gggcgttata aaacgcttgc
    30121 accagttcag gatcgcgatc gaaaccttcc ggcgttgcca catcttcaac ccgatgttct
    30181 tcccacaggc catctgcggc gcgaaaggta cgaatacctg attccgcaga aattcctgcc
    30241 cctgtcagta cgagtactct tggtttttcc attgcttccg gcaccacttt atctctgaaa
    30301 aaaatacgct gacgcaaacg ctcgcgcagg cgacgtttat ttttacgaaa acgacttaac
    30361 cgatgacccc gacgcgacag catagcaacc tctgttgttt aatcggttag atgtaggaag
    30421 gccgcaccac gcattcctcc cgcatcaccg tggcgcgcgc gttcaatgcg cggaacacga
    30481 gctacaggta agagatgaca aggcaacctg tccgccagtt gcgttgtgat tgccgggaaa
    30541 ttcgataagc caccaccaat gacgaccagg tcagggtcaa caatggtcag gatatttccc
    30601 agagaaaccg ctaataaatc cagataacgc tcaacgtgcg cccttgcctg ctcatcgcct
    30661 tgatcataaa gcgcaataat ttcgggagcc tgcaacggtt gatgataata gtgttgatac
    30721 agccacgcaa aaccgcgacc agacagataa ttttcaatgc agccatgctg accacagccg
    30781 cagcggcgta acgggaaatc caaccccatc atggttaacg catcaaccgg cagacgcata
    30841 tggccaaact cgccggtaat gtagctttta ccagtaatcg gtttgccgtt gaaaaccagc
    30901 ccgccgccaa cgccggtgcc gagaattaac cccatcacca gtggatattg cgtaaattca
    30961 tcatcccagg cttctgaaag ggcaaaacag ttggcatcgt tatcaaggcg tacatcgcga
    31021 tcaagacgtg cgctcaggtc ggcacgcagc ggtttgccgc tggcagcggg gacattagcg
    31081 gcatacagct taccatcttc tgtttccggc attcccggaa taccgatgcc gacagaacct
    31141 ttacagccaa aacgttggtc agcttcagct accagctcac acactgcatc taaaaatgcg
    31201 tcatagctgt cacgcggtgt cggcacccgc ttttcccact gcaactgccg accgctatca
    31261 aacacgccaa gcgcaatttt tgttccacca atatcaaacc cgtaatacat tgccgctcct
    31321 tgttttaatg tactgccttt actggccgct aaggactcgc gcagggtcaa tattactggc
    31381 gcgccgcgcc ggataccggc ttgccaaaag actcagcaac aatgctgtga ccagtacgta
    31441 gaagacgtcc agccagtgca attccgatgg caggaagtca ataaaataaa tatcgctgga
    31501 gaggaactga tgaccgatca gcttttcaat ccactcaata atcggggtaa gttgcagtga
    31561 aacaactacg ccgataacca ccccacacag gctgccgaac agccctgcca gcaatccata
    31621 ccagacaaag atggcgcgaa ttaaaccatc tttcgccccc agcgttctta atactgcgat
    31681 atcgccactc ttgtctttca ccgccatcac taaggtggag acgatgttga aacaggccac
    31741 gccaatcacc agtaccatcg ccagatacat aatggcgcgg atcatctgga tatcgcgata
    31801 catatagccg taagtaccaa tccaactttt aatataaaca tagctgttgg tcacttcacc
    31861 cgcatcgcgt accagcttat tggcgttgaa aacatccgtc attttaaggg caatacctga
    31921 cacgctggaa cccatatcaa gatattgttg ggcatccgcc agcgggatca tggcaaaact
    31981 gtgatcgagt tgaccactca actgcaaaat accggcaatg tgcaaacgca cacgttttgg
    32041 ctgcatcagt ttatgctcgg gattcgagtt ggggatcatg atcgacaccc aatcgccctg
    32101 cttcactttc agcgcatccg ccacgccttt gccgatgata atttgctgtt cgcccgcttt
    32161 aaaattgcgc cacgcatccc cctgaacaaa cgagggtaat gcgctcagac gctgttcctg
    32221 ttgtgggtta acgcccttca cctggattgc gcgcagattc gctccacttt ccaccagacc
    32281 ggtgaaattg atatacggcg cagcggcggc aatacccggc actttctgca cgttatccag
    32341 tgcttcctgc cagttagtcc atggctgatt taccgcttcg atttcgccat gcggcaccac
    32401 cgccagaatg cggttattca gttcgcgttc aaagccgttc atcgcgctta agccgacgat
    32461 caataccgcc acgccgaggg caatgccaat ggtagaaatg acagagatta gcgacaccat
    32521 gccgccgcgt cgccgtccgc gactaaaacg caggccaatc aataacgata aaggcatcgc
    32581 cattactccg cccccatcag gctcagttcc gccgtcagac gtccatcacg catttccagt
    32641 tggcggctca tacgtttcgc cagttgcagg tcgtgagtga ccaccaggaa agcggtgccc
    32701 tgcaagcgat tcaactcccc aagcaactgg aagatgctgt cggcgttacg cgcatcgagg
    32761 ttaccggtag gttcatccgc cagtaccaga cgcggattat tgaccagcgc acgggcaatc
    32821 gctacacgct ggcgttcgcc gccagaaagt tcagatgggc ggtgattcgc acgatgctcc
    32881 agccccaccg cttttaacat ctccagtgca cagctgttga tttcagcggg ctttttcttg
    32941 ccaatcagca gtggcatagc cacgttttcc agggcagtaa aatccggcag caggtggtga
    33001 aactgataaa taaagcccag cttctggttg cgcagttctg ctttcgccgc tgaagacagt
    33061 ttgctcatcg gctgaccgtt aaagatcaca tcgccggagg ttggcgtatc cagcccgcct
    33121 aacaggtgca gcaaggtact tttaccggaa ccagagctac cgacaattgc catcatttca
    33181 ccttcgccca cgctgaaact gacattgagc agcacatcgg tttgcacact gccttcctga
    33241 tagcgtttgc acaggttgtc gcattgcaac aggatcttat tcataacgta aagcctcagc
    33301 gggttgtgtg gcggcagcgc gccatgaagg gtaaagcgta gacagcagcg cgatagccat
    33361 cgccaccagc gcaataacaa tgacctgtaa aggttcgata gccaccggca gcgccgcgcc
    33421 atcaagcagg acgccgatta tcggcatcag attatttaac tggctggcaa gcagtgcgcc
    33481 aagcgccgct ccgaggattg caccgataat cccggcgcta gccccttgca ccataaagac
    33541 catcatgatt tgtcgcggag ttaagccttg cgtttgcagg atcgcgactt cgccctgctt
    33601 ctccatcacc atcagcccca gcgaggtaat aatgttaaac gccgcaacag cgacaatcag
    33661 gctcagcagt aagcccatca tatttttttc catgcgtacg gcctggaaca gctcgccttt
    33721 acggtcacgc cagtcctgcc atttgctgcc ttcaggcagt ttttgctgac ttaaagagtc
    33781 aactctcagc ggctcatcca gccacaaacg ccagccggta atattggctg ccggataacg
    33841 catcagacgc gaggcatcct caatattcac cagcatttca tagccatcga cttcactgtt
    33901 ggcggcgaaa gtgccaatca cattgaacag gcgctgactt ggaatacgcc ccatcggcgt
    33961 gaactggctg gcagatggca ccatcacgcg gatttgatca ccgcgattga cgcccaactg
    34021 tgaggcaagt tgttcgccga ggatgacatt atatttcccc ggctcgaggt cagtttgttt
    34081 cacattgacc agatacggcg ttagtggatc tttttgcgcc gggtcgatac cgagcatcac
    34141 cccgaccgcc acgctgcgcg cgctttgcag taccacatca ccggtagtaa taggtgcgac
    34201 gcgattaacg ccgtccagtt tgactgccgt ttccgggagt tgctgcgggt taagagagcc
    34261 atgctcagaa gagagaattg cctgtggcat caggccaagg atgttgtttt gcagctcgcg
    34321 ctcaaagccg ttcatcactg acaatactgt gaccagcgcc atcaccccga gggtaatgcc
    34381 gatggtagaa agccaggaga cgaaacgacc gaagcgatcc actgcacgcc cacgcatgta
    34441 acgcaggcca ataaatagag cgacaggttg gtacatgaaa tccgtctggt tgctgtagca
    34501 aagacacgag tatataagcg aaagcaagat gggtaaacgt ctgaactgta agtgatcgtc
    34561 agggctaatc tgaaaagccc ccgtaaatca atcggctaaa ttgccatatt ttatcatcaa
    34621 tcttgccgcc tgatcttaat ctgaaaaatc atccaataca gacagatacc cattacacct
    34681 cgggtcataa ccagcaggat caaccgcatg taaattaaaa aagagcgtgt tgttctgatg
    34741 aaacaaaaag agctatggat taaccagatc aaagggttat gtatttgtct ggtggtgatt
    34801 tatcactcgg tcattacctt ttatccgcat atgaccactt ttcagcatcc gttatcggaa
    34861 gtcctgagca aatgctggat ctatttcaat ctttaccttg ccccctttcg tatgccggtt
    34921 tttttcttta tatccggcta tttgattcgc cgctatatcg acagcgtacc gtggggaaat
    34981 tgtctcgata aacggatctg gaacatcttc tgggtgctgg cgctttgggg cgtggtgcag
    35041 tggctggcgt taagtgcgct gaaccagtgg ctggcacctg agcgcgattt aagcaatgcc
    35101 tccaatgccg cttatgccga ttccaccggt gagttcctgc acgggatgat caccgccagc
    35161 accagcttgt ggtatctgta tgcgttaatt gtctatttcg tggtatgtaa aatttttagc
    35221 cgtctggcgc tgccactatt cgccttgttt gtactgctga gtgtggcggt taatttcgtt
    35281 cccacgccgt ggtggggaat gaacagtgtg atacgcaatt tgccttatta cagccttggc
    35341 gcatggtttg gcgcaacaat aatgacctgt gttaaagagg tgccgttgcg ccgccatctg
    35401 ctgatggctt ctttgcttac cgttctggcg gtcggtgcct ggttgtttac tatctcgctg
    35461 ctgttgtcgc tggtatcgat tgtggtgatc atgaagctgt tttatcagta cgagcaacga
    35521 ttcggtatgc gctccaccag cctgctgaat gtgattggct ccaacaccat tgctatctac
    35581 accacccatc gcattctggt tgaaatattc agcttaactc tgcttgcgca aatgaacgca
    35641 gcacgctggt cgccgcaagt cgaactgaca ctcctgctgg tttatccctt tgtcagtttg
    35701 ttcatctgta ctgttgcggg cttgctggta agaaaacttt cacagcgtgc attcagcgat
    35761 ctgttgttct ccccgccttc actgcccgct gccgtcagtt actctcgtta aaccatcacc
    35821 ccagccgggg cgattcaatt tgctaaacca tgtcgattac ggataatgaa cagcattgct
    35881 tatcagagat atgcccccat atgttgaggc atatcctaac gagaatctga caaccgttat
    35941 gcctgaacaa tatcgttata cgctgcccgt caaagcgggt gagcagcgtc tgctgggcga
    36001 gttaaccggc gcagcctgtg caacgctggt agcggaaatt gccgaacgtc acgccggtcc
    36061 ggtggtactc attgcaccag atatgcaaaa tgctctgcgt ttgcatgatg aaatcagcca
    36121 gttcaccgat cagatggtga tgaatctggc ggactgggaa actcttccct acgacagttt
    36181 ttcgcctcat caggacatta tctcctcgcg cctttccacc ctttaccagc taccgacgat
    36241 gcagcgtggc atactgattg ttccggtgaa tacgcttatg cagcgcgttt gcccacacag
    36301 ttttctccac ggtcatgcgc tggtgatgaa aaaaggtcag cgcctgtcac gagatgcatt
    36361 acgaacccaa ctggacagcg ccggttatcg ccatgttgac caggtgatgg agcacggcga
    36421 atacgccacg cgcggcgcgt tgctggatct cttcccgatg gggagtgagc tgccttatcg
    36481 tcttgatttc tttgatgatg aaatcgacag cctgcgggtg tttgacgtcg acagccagcg
    36541 cacgctggag gaagtagaag cgatcaatct gctgcccgcg cacgaatttc cgaccgataa
    36601 agcggcaatt gaactgttcc gcagccagtg gcgcgatacc ttcgaagtga agcgcgatcc
    36661 ggaacatatt taccagcaag tgagtaaagg cacattacct gctgggatcg agtactggca
    36721 gccattgttc ttcagcgaac cactgccacc gctgttcagt tatttccctg ccaatacctt
    36781 gctcgtgaat actggcgatc tggaaaccag tgccgaacgt ttccaggctg acacgctggc
    36841 gcgttttgag aatcgcggcg tcgatccgat gcgcccgctg ttgccaccac aatcgctctg
    36901 gctgcgggtg gacgagctct tctcagagct gaaaaactgg ccccgagtgc agctaaaaac
    36961 tgaacattta ccgacaaaag ccgcgaatgc caatttaggt ttccagaaac tgccagacct
    37021 ggccgttcag gcgcaacaaa aagcgccgct ggatgcgctg cgtaagttcc tcgagacttt
    37081 cgacggtccg gtggtgttct cggtagaaag tgaaggtcgc cgtgaagcgc tgggtgaact
    37141 gctcgcacga attaaaattg ctccgcaacg cattatgcgt cttgatgaag ccagcgaccg
    37201 tgggcgttat ctgatgattg gcgctgccga acatggtttt gtcgataagg cgcgtaatct
    37261 ggcgctgatc tgcgaaagcg atctgctcgg tgaacgtgtt gcccgccgtc gtcaggattc
    37321 tcgccgcacc atcaaccccg atacactgat ccgtaacctt gcggagctgc atattggtca
    37381 gccggtggtc catctggagc acggcgtcgg tcgctatgcc ggaatgacca cgctggaagc
    37441 tggcggcatt actggcgagt acctgatgct cacctatgcc aacgacgcca aactgtatgt
    37501 tccggtgtcg tcactgcatc tgattagccg ttacgcaggt ggcgcggaag aaaacgcccc
    37561 gctgcataaa cttggcggcg atgcgtggtc acgcgcgcgg cagaaagcgg cggaaaaagt
    37621 gcgtgatgtg gcggcggaat tgctggatat ctacgcgcaa cgcgccgcca aagagggctt
    37681 cgcgtttaaa cacgatcgtg agcagtatca gttattctgc gacagcttcc cgtttgaaac
    37741 cacgccggat caggcgcagg ccattaatgc ggtacttagc gacatgtgtc agccgctggc
    37801 aatggatcgt ctggtgtgcg gcgatgttgg ctttggtaaa acagaagtgg cgatgcgcgc
    37861 cgctttcctg gcagtagata accacaagca ggtagcggtg ttggtgccta ccacccttct
    37921 tgcgcagcag cattacgaca acttccgcga ccgtttcgcc aactggccgg tacgtatcga
    37981 aatgatctcc cgtttccgca gcgccaaaga gcagacgcaa atccttgcgg aagtggcgga
    38041 agggaaaatc gatattctga tcggtacgca caaactgctg caaagtgacg tcaagtttaa
    38101 agatttaggc ctgctgattg tcgatgaaga acaccgcttc ggggtgcgtc ataaagagcg
    38161 cattaaagcg atgcgcgcga acgtggatat tctgacgctt actgcaacgc cgatcccacg
    38221 tacgctgaat atggcaatga gcggaatgcg tgacctgtcg attatcgcca cgccgcccgc
    38281 ccgtcgtctg gcagttaaaa cctttgtccg tgagtatgac agcctggtgg tccgggaggc
    38341 gatcctgcgt gaaattttgc gcggaggaca ggtttattat ctctacaatg atgtggaaaa
    38401 cattcagaaa gccgccgaac ggctggcaga actggtgcct gaagcacgga ttgccatcgg
    38461 tcacgggcag atgcgcgagc gcgaactgga acgggtgatg aatgatttcc atcatcaacg
    38521 tttcaacgtg ctggtttgta ccaccattat cgaaaccggg atcgacatcc cgacagccaa
    38581 caccattatc attgaacgtg cggatcactt cggtctggcg cagctgcacc agttacgcgg
    38641 tcgcgtcgga cgttcacatc atcaggcata tgcatggctg ctgacgccgc atccaaaagc
    38701 gatgactacc gatgcacaaa aacgtcttga agcgattgcc tcgctggaag atctcggggc
    38761 aggttttgcg ctggcaacgc acgatctgga gattcgcggc gcgggtgaac tgcttggcga
    38821 agaacaaagc ggctcaatgg aaaccatcgg tttctcgctg tatatggagt tgctggaaaa
    38881 cgccgtcgat gcactgaaag ccggacgcga gccgtcgctg gaagatctca ccagccagca
    38941 aacagaagtc gagctgcgga tgccgtcgct attgccagat gatttcatcc ctgacgtgaa
    39001 cacgcgtctg tcgttctaca aacgtattgc cagcgccaaa acggaaaacg aactggaaga
    39061 gatcaaagtc gagcttatcg atcgcttcgg cctgctgcca gatccggcgc gtaccctgct
    39121 ggatattgcc cgtctgcgcc agcaagcgca gaaactgggg atcaggaagc tggaaggtaa
    39181 tgagaaaggc ggggtgatcg aatttgccga gaagaatcac gttaatccgg cctggttgat
    39241 tggtttgctg caaaaacagc cgcagcatta ccgccttgat ggtccgacgc gcctgaaatt
    39301 tattcaggat ttgagtgagc ggaaaacgcg tatcgaatgg gtacgccagt ttatgcgtga
    39361 actggaagag aacgcgatcg cttaatgtaa atcgggggcg tttctgcgcc cccatctgca
    39421 acatttacaa attctttgca ctttcctgca ctatccgaca ctgtcaccat ccataattca
    39481 ggcttatctg tttattacaa taaccttata tttattatgt atttttggtg atgatcaaaa
    39541 cgcgtttttc tcgctggcta acgtttttta cgttcgccgc tgccgtggcg ctggcgctac
    39601 cggcaaaagc caacacctgg ccgctgccgc cagcgagcag tcgtctggtt ggcgaaaaca
    39661 aatttcatgt ggtggaaaat gacggtggtt ctctggaagc catcgccaaa aaatataacg
    39721 tcggctttct cgctctgtta caagctaacc ccggcgttga tccttacgta ccgcgcgcgg
    39781 gcagcgtgtt aacgatcccg ttgcaaaccc tacttccaga cgcgccgcgc gaaggcattg
    39841 tgatcaacat tgcggagctg cgtctctatt actacccgcc gggtaaaaat tcggtaaccg
    39901 tgtatccaat aggtattggt cagttaggtg gtgacacgct gacaccgaca atggtgacca
    39961 ccgtttcaga caaacgtgca aacccaacct ggacgccaac ggcaaacatc cgcgcccgtt
    40021 ataaagcaca gggaattgag ttgcctgcgg tagtgccggc tggaccggat aacccaatgg
    40081 gccatcatgc gattcgtctg gcggcctatg gcggcgttta tttgcttcac ggtacgaacg
    40141 ccgatttcgg cattggcatg cgggtaagtt ctggctgtat tcgtctgcgg gatgacgata
    40201 tcaaaacact ctttagccag gtcaccccag gcaccaaagt gaatatcatc aacactccga
    40261 taaaagtctc tgccgaacca aacggtgcgc gtctggttga agtacatcag ccgctgtcag
    40321 agaagattga tgacgatccg cagctgctgc caattacgct gaatagcgca atgcaatcat
    40381 ttaaagatgc agcacaaact gacgctgaag tgatgcaaca tgtgatggat gtccgttccg
    40441 ggatgccggt ggatgtccgc cgtcatcaag tgagcccaca aactctgtaa atcctggata
    40501 aaaaaaatcc ccgcgacacg gcatgtcgcg gggaaaaaat cagcagtagc aggcattaat
    40561 gagggttaat gcttatttat aaattactgc tgttccatgg agggtattcg gaccggttac
    40621 agaagtaata cggaaagatt ttgcgcccat ctcatccgct ttttgcgcca gctgctcttc
    40681 cagcgatccc agatttgtcc ccgcgttagc actgatagta ccgacttttt gttggccttc
    40741 tggcgttgac tgaacttcga cagccgcaaa gttggcaaat gacatggagc ttaaaatcgc
    40801 cgcagcgatg aggtttttac gtttttcata atagtggcct tatgcagatg aatgacgtcg
    40861 caagaaatta cttaacgatc gataactaaa tgatagatgt gatctggatc acatacaaga
    40921 tattttttat aacaatcatt aattaattta aaaatcttta aatttcagtc aaatagaaag
    40981 caattatttt tatgggatct ggactggtga acggaggtga ttatttttat aataaccttt
    41041 cattcacagt caccaggtac aacggcatca tggcaactga ctcaacacaa tgtgtaaaaa
    41101 aaagccgtgg ccgcccaaaa gtgttcgaca gggatgccgc gcttgataag gccatgaaat
    41161 tgttctggca acacggttat gaagcgactt ctcttgcgga cctcgtcgaa gcaaccggag
    41221 ccaaagcgcc cacgctatac gcggaattta ccaacaaaga ggggttattt cgtgccgttc
    41281 tcgaccgcta tatcgatcgt tttgccgcta agcatgaagc acagctgttt tgtgaagaga
    41341 aaagcgtgga gtctgcgctg gctgactatt ttgctgccat cgccaactgc tttaccagca
    41401 aagacacccc ggcaggctgc ttcatgatca acaactgcac caccctctcc ccagattcag
    41461 gagatatagc caatacgttg aaatcacgcc atgcgatgca agagcgcact ttgcagcagt
    41521 ttttatgtca acgacaagcg cgcggggaaa tcccgaccca ctgtgacgtg acacatctgg
    41581 cagaattcct taattgtatt attcagggga tgtcgatcag cgcacgcgaa ggtgcatcgc
    41641 tggaaaaact gatgcagatt gccggaacga ctttgcgttt atggcccgaa ctggtgaaat
    41701 aagtcgcaaa aaaggcccct cagcctgagc gaatgagggg ccaaattgca gagtacagcc
    41761 ttgttatact ttgttattca aaatcagttg cccgttacta tctagcggga tctgcgtacc
    41821 cggatcgcgg tccatgcgga ttttgccctg ctgatcgcca atcttatagg tcacatcata
    41881 accgagcatt ttttctgact tgtcatacac cgttttacaa cgctgttgcg tagtcgtgta
    41941 agtatcgctt tcctggagag agccctggat ctggttaccg gcatatccac cacccagcgc
    42001 ccccacaaca gtggcgacat ctttaccgcg accaccacca aactgatgcc ctatcacgcc
    42061 accagcaaca gcgccgagca ccgacccggt aatgcgattt tcatcctgca ccggtcgacg
    42121 atgggtcact gtgacgttgc gacactcctg acgcggtgtt ttaaccgttt ccttgattgg
    42181 ggttgcagaa acaacctgag cgtattgcgg gccccgttca aacacgttca gactggccac
    42241 tgccgctacg cccaacgcag ctgcgacacc aatcccgata cccgccaaca ttgatttatt
    42301 cacgggaaca cctccttctt tgcagttatg ccaatcatgc aagcgaagga gaaaagcagc
    42361 tatcagacag aagatgaaaa gcgcgggaat gactggaaat acttaaggat tcggaggagt
    42421 ctgaaggaat gcctgatgcg acgcttaacg cgtcttatca ggcctaccgc tcgtaccatt
    42481 tgtccagggc ggataaggcg tttataccac atccgccagt gtacgtcgat taatgcaact
    42541 tcaaacgcgg acggataacg cggttaatac tccccaccag catcattaat ccggttttaa
    42601 agtaaccatg cagcgcaatc tgatgcattc ggtatagcga gatatataca aagcgcgcaa
    42661 ttcgtccttc aatcatcatt gagccgcgcg tcaggttacc catcaggcta ccaacggtgg
    42721 agaagttcga cagcgatacc agcgaaccat gatctttata ctgataattt ttcagcggct
    42781 taccgttcat ctgcgccaga atgttgttca ttgcgcaagt cgccatctgg tgtgcagcct
    42841 gagcacgcgg cggaacaaag cccccttccg gacgcgggca tgacgcgcag tcgccaatag
    42901 cgtaaatgtc tggatcgcga gtggtttgca gcgtcggttc caccaccagc tggttgatac
    42961 ggttagtttc aagaccaccg atatctttca ggaagtctgg cgctttgatc ccggctgccc
    43021 ataccatcag atcagcctca atatattcgc catctttagt gtgcaggccg ccttcatcag
    43081 cactggtgac catggtttgc gtcagcacgc gaacgccaag tttcgttagc tcgttgtggg
    43141 ccgcagcaga gatacgtggc ggtaatgcag gcaaaatacg ttctcccgct tctaccagcg
    43201 ttacgttcag ggcttcgttg gtcaggcctt tgtaaccgta gctgtgcagt tgcttgatcg
    43261 cgttgtgcaa ttcagcggag agttctacac ccgtcgcgcc gccgccgaca atcgcaatgt
    43321 tcactttgcc atttgcgccc aggttggcag agtatttcag gaacagattc agcatctcct
    43381 ggtggaagcg acgcgcctgg tgcgggttat cgaggaaaat gcagttctct ttgacgcctg
    43441 gcgtattgaa atcgttagag gtgctaccca gcgccatgac cagggtgtca taggcgattt
    43501 tacgttccgg aaccaacagt tcacctttct cgtcgcgtag ttctgcaata gtgattgttt
    43561 tcgcttcacg atcaatatca atgacggaac ccaactggaa ctggaaacca tgattgcgcg
    43621 catgggccag atagctcaac gcatcgacgc cttcatcaag cgagccagtc gccacttcgt
    43681 gcagcagtgg tttccacagg tggctgtggt tacgatcgac cagcgtaatt ttggcttttt
    43741 tcttacgtcc cagcttatgc cccagctgtg ttgccatttc cagcccacca gcaccgccgc
    43801 cgacaatcac aatctttttc aatggcgtag tcaacgtgcc ccccttaaat ttattaacca
    43861 attgttaatt aatggttatt aacatagctc ttaattaaca acaggttaca gcaatgaaaa
    43921 tgttcttcgc tattgagaat aacatgaatg gtgcattggt cataccaaaa ttgatgttaa
    43981 tcaagttttg ttgcttaaaa gataggcaac tttaccgggg gagatcaaaa aatagagttt
    44041 attgtttatt atttgagcaa attcggaagg gtaatatagg gtgaagcgta tatcgagggc
    44101 gtttggtcgt cctgataaga tgcgcagcat cgcatcaggc attgatgctc gactgtcgga
    44161 tgcggcgtgg acgccttatc cgacttacag aattcggagc gcatcaggta ccggcaaggt
    44221 tacctgatgc gacgagggca tttacccgag ggttttgaac gctttaatgc gctgtaaatg
    44281 cggggagata ttcttgaatt tgtgcgtctg ctcttcgtcc cagacaatct cgtaataatg
    44341 atgcaactct tcagatgtcc gctggctgtt aagtgcttca tcattacgcg acaaaataac
    44401 cagacaacga tcgcgattct tctcgcggaa gttggtcaca cacttagtgg caatatcggc
    44461 atactcttcc gggcgatcaa tcttgccttc catgttctca taagggaaca aattagggtt
    44521 gaagatcacc tggcggatat cgcaaagaaa accaatccgt tccgcccagt atccgcccag
    44581 gccaacgccg caaattagcg gacgctcgtc aacgttcagt tgcaacattt tgtccacttc
    44641 tttaagcaga tgctgcatat catgtttcgg atgccgcgta ctgtagctta tcaagcgtac
    44701 atccgggtca ataaactgca attgtaagac tttctcgtgg ttacccggac tgttagagtc
    44761 aaaaccgtgt aaatagataa tcatcgcttc ctcacataag ccagggttag tgacctactt
    44821 tctcttcctg ccagcgttca tgtaactgat tcagacgggt gctgatcgct ttccagcgag
    44881 ccgagtccat cagttcctgt cgcgaaaatg aacctttatg atacaaacgt gtaacacgtt
    44941 ctgccttgat cggtgacaga ttatctaaca cgctgacggc ccctttacga ttattgcaga
    45001 ccaggatcat atcgcaaccc gcatccagtg atgcctgccc gcgttcggca taactgccca
    45061 taatcgcggc accttccatc gataaatcgt cagagaaaat cacgccgtca aaccccagtt
    45121 cctgacgcaa aacggttttc agccagtagg gagaaccgct cgccggacgc ggatcaacat
    45181 cactgtagat cacatgcgca ggcataatgg cgtcgagttt attttcgcgg attaaggaac
    45241 tgaagaccga catatcttta gcgcgaatct ccgcttgtgg acgtggatcg cacggtgttt
    45301 ctttgtgtga gtctgccgtt actgcaccgt gtcctgggaa gtgtttcccg gtcgttttca
    45361 ttccggcttc atgcatacca tcaataaacc ggctggcaat tgccagggct ttttgtggat
    45421 cggcatgata agaacgctcg ccaatcgccg cgctgatatg cccgacatcc agcactggcg
    45481 caaagctgat atcaatatcc atagcgatca tttcgctggc catcagccaa cccgcctctt
    45541 gcgccagttt gccgccctct tccattccca acagcgcagc aaaggattgt gctgccggta
    45601 agcgggtaaa accttcgcga aaacgctgca cgcgtccacc ttcctgatct accgccacca
    45661 ccagatgatt gcgcgatgct gcgcggatct ggcgcaccag ttcacgtaac tgggcaggat
    45721 catgatagtt acgcgtaaag agaatcagcc ctcccaccag cggatgcgcc agtatttcac
    45781 gctcttccgc gtccagttcg taaccttcga catccaacat tactggaccc acactgctct
    45841 ccttattgtt ttattaatag ctgccgccag gtgtcatcgg ccagcctgat aaattgttga
    45901 tcgccggttt gtcgccagcg gtactcaaac caccctcctt tgagcatcag cagccaggga
    45961 aaccatcgcc tgacctgacg ccataattgc gccggataaa tcttcgcgcg agtggcatag
    46021 tcattgacca attgccggtg ctgttcagta ttttccaccc acaccgccgc cagttccagc
    46081 gcgatatcac catctccagc atactcccag tcgatgagtt ttaaccctga tgcgctatgc
    46141 actaaatttc cggcgtggac atccatatgc aatggactta agcgtaaagg tcgtggttcc
    46201 cgcgctttgc gcagacgttt taacattcgc agccaaccca cagtccgccg cgccggatcg
    46261 ctttgctgcc agtacagttc cagtaagggc aacagcgtta ttcgccagcc aaaacgtggt
    46321 tgttgatgta gataatacag caagcctgcc agttcgttgg tatccggcaa atacgttttt
    46381 accgcaccgg gcagatagtc gactaccatc cagtcacgga gatataaatg cggcttcggt
    46441 gcaatgcttg cgggtagttg tgataaagcc cgatactggc gcaagaacgc ggactgcggc
    46501 gcatcaggat cgtgcggctg acgcacaaca aaacgctggc cctgatgttc aatgagaaaa
    46561 ctaccgccac taagcccact attaaacgtc gtgacgggat gaaactgcgg gaaaaagcgc
    46621 gacagcaatt cgtcgcgcgt gatgggatta ttgctgcgaa acggcacctt tacctgacca
    46681 gataatttcg cccgtctgca ccagcatcag ctgcatttgt agggtcggag cgttaacgtt
    46741 gccagaggcg caggagtaca gcacgtaatg agcgccgaca ttgcgggcaa tgcctatggc
    46801 tttactacgg gtgcctaaac tgtcctgcgg cgacaaacct aactgttgct tagccatcga
    46861 cagttgctgg gcggaaacca gggtaaattt cccgttatta gccagcgcat ttcgcagcgt
    46921 ttcggtcgct tctgcggcat tcagcgaacc gttagtacgg ttgttaacgc tatcaaccag
    46981 caggacgcta cccgcagtca ccccgtcagc cccaagcatc ttactgacca tcggctgcat
    47041 tgcgccattc cagtcataat ggcgaatatg cggcgcaggc ggtgccgttc gatcttcgtg
    47101 ctcaattggg tctggctgct gcgggatcgt cggcaccgag ggcactgtgg ggacaggctg
    47161 ttgtggctcg gctggttgtt ccggcgctgg tttcacttct tctaccggtg caggttcacg
    47221 ttgccccaca cacccggcga gaaacatcgc cagcgcggta atcaaggcgt agcgactcat
    47281 ttttgtcatc aagattcacc cctttacaaa tatagataaa gtctgacttt gtgcgccccc
    47341 agaaaattgg cgctgccgta cagcgttacc gccgaatgtg cgggaatggt aacgctgcgt
    47401 ggcctttcca gaggatgcat ctccagccct ctggcgtcat accagtaaaa acgataatga
    47461 acggtaacgg gttcttgcct ttcgttataa atcgttgagg atgctgaagg ttgaatatca
    47521 gacgtcgaaa ggacgggctt ttctgcactg atgcccgcag ccaataaaga tgactccatc
    47581 accagcgatt gctcatcatt caccggaatt tccggatgtg aacgacagcc caccagcagt
    47641 aacaacacca gagacaccag cccaaagcat ccttttctca tcgttacaga cctttatgcg
    47701 ccagcattgg tcccagcgga cggccaccca acaagtgcat atggatgtgg taaacctctt
    47761 gtccgccatg gcggttggta ttcatgatca gacgatagcc atcttcggca ataccttctt
    47821 gctcagcaat ttttgccgct acggtgatca tgcgtcccag cgcctgctca tgctcagctg
    47881 acacgtcgtt cacagtcgga atgagaatat tcggaatgat cagaatatgc gttggcgctt
    47941 gcggcgaaat atcgcgaaac gccgttacca gatcatcctg gtagacgata tcggagggga
    48001 tctcacgacg aataattttg ctgaatatag tttcttctgc cacgacattt tcctttttca
    48061 aatagccatg cgttgcgcca tgctacgccg cgccacactg cgagtataga gtatggtcga
    48121 gttaaacctg ctctttcaat cttaactcac gatttcttaa gcaaaaaatc gttttgctgc
    48181 cgtatatatc gccattattc ccatttctgc ctgcgaatgc gctgaaaaga cattctctgt
    48241 ttggtttaca cgacaaatat gaatgcgtat atttctcatt tgcatttgca tttgcatttg
    48301 catttgcagc aaaattattc gcacactaaa tataaccgct catcaccctc ctccggcatg
    48361 agcctgacga caacataaac caagagattt caaatgcttt caacacaatt taacagggat
    48421 aatcaatatc aagtcatcac caaaccgtca ctacttgccg gttgcatagc actggcacta
    48481 ttaccttctg ccgcttttgc tgcaccagcc actgaagaaa cggtgattgt tgagggttca
    48541 gccacagctc cagatgatgg cgaaaatgat tacagcgtaa cgtctacctc tgcgggtacc
    48601 aaaatgcaga tgactcaaca tgatattcct cagtcggtca ctattgttag ccagcagcgg
    48661 atggaagatc agcagttaca aacgctgggc gaagtgatgg aaaacacgct ggggatcagc
    48721 aaaagtcagg cggattccga tcgtgctctt tattattccc gcggattcca gatcgataac
    48781 tatatggttg atggtatccc cacctatttt gaatcgcgct ggaatctggg cgacgcactt
    48841 tctgatatgg cactgtttga acgcgtagaa gtagtgcgtg gcgcgacagg actcatgacc
    48901 gggacgggta atccatctgc ggcaattaat atggttcgaa aacacgcgac ctgtcgtgaa
    48961 tttaaaggcg atgtctcggc ggaatacggt agctggaaca aagaacggta tgtggcggat
    49021 ttacaaagcc cactcaccga agacggtaaa atccgcgcgc gaattgtcgg cggctaccag
    49081 aataacgact catggctgga ccgctacaat agtgaaaaga ccttcttctc gggcattgtc
    49141 gatgctgatt taggcgatct tacgacgctt tcagccggtt acgaatatca gcgcattgat
    49201 gttaatagcc caacctgggg cggtttaccg cgctggaata ctgatggcag cagcaacagt
    49261 tacgatcgcg cacgcagtac cgcacctgac tgggcgtaca acgataaaga gatcaacaag
    49321 gtctttatga ccctgaagca gcggtttgct gatacctggc aagcgacact gaatgccacc
    49381 cactctgaag tcgaatttga cagcaaaatg atgtatgtcg atgcctatgt aaacaaagcg
    49441 gatggtatgc tggttgggcc atacagtaat tatggacctg gctttgatca tgtcggcggc
    49501 accggttgga acagtggcaa acgtaaagtt gatgcgctgg atttgttcgc tgacggtagt
    49561 tatgaattgt ttggtcgtca gcacaatcta atgtttggtg gcagttacag caaacaaaac
    49621 aatcgttcag ttcatgggcc aacatcttcc cggatgaaat tggcagtttc tacaacttta
    49681 atggcaattt cccacaagcc gactggtcat cacagagcct ggcgcaggac gataccacac
    49741 atatgaaatc gttatatgct gccactcgtg tcacccttgc cgatccgctg catctgatcc
    49801 tcggcgcacg ttataccaac tggcgggttg atacgctgac ttacagcatg gagaaaaacc
    49861 acaccacgcc ttacgctggt ctggtgtttg acatcaatga caactggtcg acctacgcca
    49921 gctatacctc tattttccag ccgcaaaatg atcgtgacag ttcaggcaaa tatctggctc
    49981 caatcaccgg taacaactac gagctgggtc tgaaatcgga ctggatgaat agccgtctga
    50041 ccaccacgtt agccatcttc cgtattgagc aggataatgt cgctcagtcc accggtacac
    50101 ctatccccgg cagcaacggc gaaaccgcct ataaagcggt ggatgggaca gtcagtaaag
    50161 gggtggaatt tgaactcaac ggcgcaatta ccgacaactg gcagctgaca tttggcgcaa
    50221 cgcgctatct tgcagaggat aacgaaggaa acgccgttaa tcctaatctg ccacgcacca
    50281 cggttaaaat gttcaccagc tatcggttgc ctgtcatgcc agagttgaca gtcggcggtg
    50341 gtgttaactg gcaaaatcgc gtgtataccg acaccgtgac accgtatggc accttccgcg
    50401 ccgagcaagg tagctacgcg ctggtggatc tcttcacccg ctaccaggtg acgaaaaact
    50461 tctcgttaca ggggaacgtc aataacctgt tcgacaaaac ctacgatacc aacatggaag
    50521 gttctatcgt ctacggcgca ccgcgtaatt tcagcattac cggcacgtat caattctgaa
    50581 taacacctgt aaaaaaggca gccatctggc tgccttagtc tccccaacgt cttacggatt
    50641 agtggttacg gatgtactca tccatctcgg ttttcaggtt atcggattta gtaccgaaaa
    50701 tagcctgaac accagaacca gcaaccacta cgcccgctgc gcccagtttc ttcaggccag
    50761 cctgatccac tttagacaca tcagcaacgc tgacgcgcag acgggtaata catgcgtcga
    50821 ggttagtaat gttttcttta ccaccaaatg cagcaaccag agccggtgcc atttcgctgg
    50881 tacctgttgc ttttgcatct tcagtcgcgt cttcacgacc cggcgttttc agatccagtg
    50941 ctttaatcag cacgcggaag atggtgtagt aaacaatcgc ataaccgata ccgacgatcg
    51001 gtaacagcca cagtttgctg ctgttaccag acagaacgat gaagtcgatc agaccgtgcg
    51061 agaacgacgt accgtcacgc atccccagaa gaatacagat tgggaatgcc aggcctgcca
    51121 gaatcgcgtg gatgatgtac aggatcggcg caacgaacat gaagaagaac tcgatcggct
    51181 cggtgatacc ggtcaggaac gaggtcagcg ccgcggagat cataataccg cccactttcg
    51241 cgcggttttc tggtttagca gagtgccaga tagcaattgc ggcagctggc agaccgtaca
    51301 ttttgaacag gaagccacca gacagtttac ccgcagtcgg gtcacccgcc atataacgcg
    51361 gaatgtcgcc gtggaaaacc tgacctgctg cgttggtgta ttcaccaatc tgcatctgga
    51421 aaggtacgtt ccagatgtgg tgcagaccaa acggtaccag gcaacgttcg atgaaaccgt
    51481 aaatgccaaa cgcaactacc gggttctggt aagcagccca ctgagagaag gtctggattg
    51541 cagaaccaat cggcggccaa atgaaggaca gcacaacgcc agtaaagatg gcagccaggc
    51601 cagaaatgat cggcacaaag cgtttaccgg caaagaagcc aagatactca ggcagcttaa
    51661 tacggtagaa acggttaaac atgtacgctg caatcgcacc ggagataatc cctccgagta
    51721 cgccagtatc cgccaagtgt ttagaggcga tttcttcagc aggtaaatgc agtaccagtg
    51781 gcgcaaccac ggccatggtt ttaaccatga tgccataggc aacaactgcg gccagcgcgg
    51841 atacgccatc gttattggta aagccgaggg cgacaccgat cgcaaaaatc agtggcatgt
    51901 ttgcaaagac ggaaccgcct gcttctgcca taacatgcga tacaacggcg ggcagccagc
    51961 tgaaattcgc ggaaccgacg cccagcagaa tacctgcgat aggtagtacg gataccggca
    52021 gcatcagcga tttaccgacc ttttgcaggt tagcaaatgc attcttaaac ataattgaga
    52081 gtgctcctga gtatgggtgc tttttttacg ttctcacgcg tggcaagggg ggagagcctc
    52141 gccgtgtaca gggcatctaa gcgcccttta tttattacac agagtaaaat aattcagtgc
    52201 caatttgttt gacggctatc acgtttcaac ttcagatcat tggagaagtt gcacattttg
    52261 acaaaacata tgtcacaata tttcaaacgc ggttattcac cgcccaattt tcggcggtga
    52321 actttactcg ttttagccat taattacgag ctttaaaaaa actcattcaa cggatggatt
    52381 gaaggcggga agcgtcgatg tgaaacagac gggcgaagtt atcggtggtt acctgcgcca
    52441 gttcttcaac ggcaacacct ttcaacacag ccatgtattc tgcaacgtca cgaaccatcg
    52501 caggttgatt ctcttttcct cgatgcggta ccggcgcaag gtaaggtgag tcagtttcca
    52561 ccagtaatcg atccaggggg acataacgcg cagcatcgcg cagttgctcc gcattacgga
    52621 aggtcacaat gccggaaaag gagatgtaaa atccgagatc cagtaattta cccgccgttt
    52681 ctctgtcctc tgtaaaacag tgtagtacgc caccgcaatc cgtcactttt tcttcgcgca
    52741 gaattgccag cgtatcggca cgggcgtcac gggtatggac gataaccggc ttgttcagtt
    52801 cacgaccaat ctggatatga tggatgaagg actcttgctg acgtacttta gtttccggtg
    52861 tgtaataata atccagcccg gtttcaccca gcgctacaac accctcttct gccgccagac
    52921 ggcgtaaatc ttctacatcg tagggatcat tctggttgag tggatgcacg ccacaagaaa
    52981 atacgacgtt gtcacgttcg cctaccagat cccgcatatg taaataaccc ggtaatgttg
    53041 tggcgactgc cagacaaaat ttcacatcgc gtgcggcggc tttcgccaga acgtcatcca
    53101 cgtccttatg caaagattca taatccagac catcgagatg gcagtgtgag tcgactaaaa
    53161 acatgatgtc tctcttaaag atgaggaacc ggtaacacaa cgcccggttg caggtaatgc
    53221 tcaatacgca gtaaaagatc ggtgatgaga agctcgcggt tgatgcctgt gacagacatt
    53281 aactgttcac gaatgtggca aacatccccc agtatagcct gcaggcacga gggagaaaga
    53341 tggtttgcca gttcggcgac caggcccggc acatcaacat tggtcacctg cgcagcgcca
    53401 tgatggcgtt ttagcgcatc catcagcaac gttgccagcc agtgtaaacg cgccggagct
    53461 tgttcatgat taagggccgc cagcagcgaa taccagtcgc ccgatggcac gctatatgcc
    53521 aacgcctgac acaatgtttc acgagcctgc cagttatctc cctgaaacaa cgccagtgcc
    53581 gcgccaggcg aaccggcgct taagcgcaat gcggcaagta atgcatcctg tgacattgtc
    53641 acttcgcgtg aaagccaagt cacggcgtac tgttctggag gcggcgcaag gtaatgtaac
    53701 cgacaacgac tacgtaatgt tgccagtaaa cgttcaggct cgcgggtagc caggaaaaac
    53761 caggtttctg ctggtggctc ttcaagcgtt ttcagcaatg cgttagccgc ggcgtcggtc
    53821 agtaaggcag catcggttac ccagacaact ttcgcaccac ctaggcgtgc gtgctcattc
    53881 agcttttcgg tgacctcacg taccgcatca atgcccagcg tatttttccc tttttcggga
    53941 gccagggtgt agtaatcggg atgcgtgcca gcctgcatca actgacatcc acgacagtga
    54001 ccgcaacttt tgtggccctg cggttgttgg cagagtaaat aacggctcag ggcgtagatt
    54061 aaagcatcat cgcccatgcc cggtaacgcc tgaatgagta gcgcatggtg acctcttccg
    54121 gcctgatagc tggctaccag tttttcgaaa tcaggtcgta accatggata ccatctcatg
    54181 cgtccaactc cttcacccag tgggtcacgg tagtgcggat tgcatccatc acggcctcca
    54241 gcggctgggt ggcatcaatg gtatgaatac ttttatcttg tgcagccagt tccagatagc
    54301 gggcgcgggt gcgattaaag aaatcgaaag actcttgctc aatacgatcc agctcgccgc
    54361 gcgcacgcgc gcgttttaag ccaacttccg gggtaacatc gagatagagc gttaagtctg
    54421 ggcgaaaatc cccgagaaca gcatcacgca gtgttgccag catatgttgg tcaataccac
    54481 gtcctccgcc ctgatacgcc tgagtggaaa gatcgtggcg atcgccaatc acccaggtgc
    54541 cgttagccag cgcaggtttg atgaccgttt ctaccagttg aacgcgcgcg gcataaaaca
    54601 tcagaacttc ggctttatcg gtaatgactt catcgcctac cgatttgata tccagcacca
    54661 ggcttcttaa cttttcggca agttgcgtac cgccaggttc ccgagtgaaa accatgtcgc
    54721 ggatacccag ttgctcgagc gtctcaacca ccacattacg cgcggtagtt ttgcctgcgc
    54781 cttccagccc ctcaatgacg atatacttac tgcgcatttt tttccttaag cactttcaga
    54841 taatcctgca cagacttgtt atgactggca agattggtat taaacgtgtg accaccttta
    54901 ccatcggcca caaaatagag atacggcgtt tttgccggat gcgcagcagc cttcagcgaa
    54961 tccgcccccg gcgtagctat cgcacccggc ggcaaaccgg taatggtata ggtgttatac
    55021 gctgtcggcg tttccaggtc tgcacgagaa agtttgccat tataacgctc tcccatcccg
    55081 taaatcacgg ttgggtcggt ctgcaggcgc ataccaatgc gtaaacggtt gataaatacc
    55141 gaggcaacct gatcgcgttc actggcaacg gcggtttctt tttcgataat tgatgccatc
    55201 gtcaccagct gatttttatc tttataaggc agaccgtccg cacgcccttc ccaggcgcta
    55261 tcgaccgctt tcaccatttt cttgtgaact cgcttgagta acgcgacatc ggtggtattg
    55321 gcggtataca tcaaggtgtc tggccagaac caaccttcaa tccactccgg gttttccagt
    55381 tcaagtgcct gcgctacggt ggcgtactta tcgtcactca gcgtgtgctt gatatacgga
    55441 gcctcacgca attgcttgag gtagtcgctc agacgcatcc cttctaccag tcgcagcggg
    55501 aactgtgctt ctttaccgct ttccaacaat ttcagcatct cgcgcacggt catctgcggt
    55561 gtaaagcggt aagtcccggc tttaaagtga gaaagatccg gttcgatacg cagcagccat
    55621 tgaaaaaccc gtgggcgatt gatgatctta tcagcataaa gctgttcacc gagcgccaga
    55681 cgtccggtcc ctggcttcag ggtaaatatc gtctcttctt tgataagcaa tttgctgtcg
    55741 gcaagatggc gaaccttcca gacgcccaca ccagcggcga tacccagtac caccagcaat
    55801 aacaagataa tcaataacac ttttttcatg actaattcgg gcgctcacaa agtggggcta
    55861 aatattcata taacgttgct gacgaaaagg tgacatcgcc acaggcacgt acgggcatca
    55921 ctggcattaa cgcattacaa ataaccatct catctgcctg caacgcctcc tccagcgagg
    55981 cttgcacttc gacaagctga taagaggatt gtgccagtaa acggatacag aattgtcgca
    56041 taatgccgtt aacacctgcc tgatccagtc gcggcgtata aactacattg cccttccgcc
    56101 agaacaaatt agccgcacag cattccgtaa cccacccttc gctgtcaagg accagcgcct
    56161 catcagcgtt tgtctgctca agatgagagc gaatcaatac ttgctcaagc cgattaagat
    56221 gtttaatacc tgcaagatga ggattgcgcc ccagccgcac cgggcttagc gccaacgtca
    56281 tcccttcgtt acgcaaacgg tcgtaatgtg caggataagc cgtaacggag agaatccgcg
    56341 ttgctggtcc gctgttcaat gtgctgtacc ctcgcccgcc actaccgcga ctgatcacga
    56401 ctttcagcac accattttgc tgttctgctg ccagcgtttt catctcttgt tcaagctgag
    56461 gccagaagtc acaggaaatc atcaaccgct gacaagcatc ctgtagtcgc aggatatgcg
    56521 ccgataacaa actgacttta ccgtcgataa ctctggcggt ggtaaaacaa ccatcaccaa
    56581 actgcgttgc ccgatcgctt actgccagcg attcctgctt ataaccgtta attaagaaca
    56641 taccggctcc ttatcagaga attgttagtg tggcagcatg ttcactacgg aacaagtcgg
    56701 aataaaagct aagaaaaaag gcccgcaagc ggacctttta tatgggtgga aaatgacaac
    56761 ttagatcttt ttaaagatca aagaaccatt agtgccaccg aagccgaagg agttacacag
    56821 cgtgtattcc attccgctga cctgacgtgc ttcatgcggt acgaaatcca gatcgcaacc
    56881 ttcatccggg ttatccaggt tgatggttgg cggaacagcc tgatcgcgca gcgccaggat
    56941 ggagtagata gattctactg cacccgccgc acctaacagg tgaccggtca tagatttcgt
    57001 ggagcttacc aacacacggc ttgcagcttc accgaagatg gttttcaccg cctgcgcttc
    57061 agctttatcg ccagccggcg tagaagtacc gtgcgcgtta acgtagccaa tctgactcgc
    57121 ttcaatgcct gcatcacgca gagcatttgc cattgccaga gctgcgcctg cgccattttc
    57181 tggcggtgac gtcatatgat aagcatcgct gctcatacca aagccgacga gttcagcgta
    57241 aattttcgca ccgcgttttt tcgcgtgttc gtactcttca agtaccagca taccggcacc
    57301 atcgcccagt acgaagccat cacgctcttt atcccacggg cggctcgccg cttgcgggtt
    57361 atcattgcgg gtagataatg cacgtgccgc gccaaaacca ccaacgccca gcggcgtact
    57421 ggctttctct gcgccacctg caaccatcac gtcagcatcg ccatacgcga taatacgcgc
    57481 agcatggcca atgttgtgca cgccggaagt acaggcggtc gcgatagaga tgcttgggcc
    57541 acgcaggcca tacatgatag tcagatgacc tgccaccatg ttcacaatcg ttgacggaac
    57601 gaagaatggg ctgatcttac gtggaccacc gttcatcaga gatgtgtggt tttcttcgat
    57661 cagtccgagg ccgccaatcc cggagccaat tgcggcacca atgcgggttg cgttctcttc
    57721 cgttatttca aggccagaat cctgcatggc ctgaacgcca gcgacaattc catattgaat
    57781 gaaggcatcc atcttgcgct gttctttgcg cgagataatg tcctcacagt taaaatcctt
    57841 tactaagcca gcaaatttcg ttgcataggc gctagtatcg aaatggtcga ttaggctgat
    57901 gccactctga ccggcaagca gagctttcca ggtagactct acggtattgc cgacaggaga
    57961 caacatgccc agtccggtca caactacacg acgcttagac acgtttgtcc tccaaggagg
    58021 gaaaaatgat tctagtggga caaaaagata aaactcaggc ggtcgaacga ccgcctggag
    58081 atgttcactt acgcctggtg gccgttgatg taatcaatgg cagcctgaac ggtggtgatt
    58141 ttctcagctt cttcgtccgg aatctcagta tcaaactctt cttccagagc cattaccagc
    58201 tcaacggtgt caagagaatc cgcgcccagg tcttcaacga aagaagcatt gttggtaact
    58261 tcttcctgct taacgcccag ctgttcgccg ataattttct taacgcgttc ttcgatagtg
    58321 ctcatactct taaatttcct atcaaaactc gctttcgcga tggttttcgt agtgtataaa
    58381 atgttgaaaa atttgcaact aaatcccggc aggtcttacc acgattttac gttattttga
    58441 ggccttaccc cccaataacg caaatcattt tgcactaatt attactcata accacgcaga
    58501 ctgcgcgcaa catgagcatt ttgtgcaaat cgcggtcaga ccatgtacat cccgccgttc
    58561 acatgcaaag tttcacccgt gatgtaagct gcttcgtcgg atgccaggaa tgcaaccgcg
    58621 ttggcgattt cctgtgcgcc gccgaggcga cccgcaggaa cctgcgccag gatacccgca
    58681 cgctggtcat cgctcagcgc acgtgtcatg tccgtttcaa taaagcccgg agcaacaacg
    58741 tttacagtaa taccgcgtga cgcaacttcg cgcgccagtg atttactgaa gccgatcaag
    58801 cccgctttcg ccgcagcgta gttggcctga ccgccatttc ccatggtacc aaccacagaa
    58861 ccgatagtga taatacgacc atgacgcttt ttcatcatag cgcgcattac cgcttttgac
    58921 agacggaaaa cagatgaaag gttagtttcg ataatatcgt tccactcttc atctttcatt
    58981 cgcattaaca ggttatcacg agtgataccg gcattattga ccaggatatc cacttcacca
    59041 aattctgcgc gaattttttc cagaacagat tcgatagatg ccgggtcggt cacattcaac
    59101 atcagacctt tgccgttggc acctaaataa tcactgatcg cctgagcgcc attttcactg
    59161 gtcgcagtgc caataacttt cgcgccacgg gctgcgagcg tttcagcaat tgcgcggcca
    59221 attccgcggc ttgcaccggt taccagtgcg atttttcctt caaaattcat gattttcctc
    59281 ttttaaagct cgagcgccgc tgccatcgct gaaggttcgt tcagcgccga ggcggtcagg
    59341 gtgtcgacaa tgcgtttcgt caggccagta agcaccttgc ccgggccgac ttcatagaga
    59401 tgttctacgc cttgcgctgc catgtactca acagacttcg tccactgaac cgggttatac
    59461 aactgacgta ccagtgcgtc acggatggca tcaccattgg tttcgcattt cacatcaacg
    59521 ttattcacaa cagaaactgt tggtgcgtta aaggtgattt tcgctaattc tactgccagt
    59581 ttgtcggctg ctggtttcat cagcgcacag tgagacggta cgctcactgg taacggcagc
    59641 gcgcgttttg cgccagcagc tttacaggca gcgccagcac gctcaaccgc ttctttatga
    59701 ccggcaataa ccacctgtcc cggagagtta aagtttaccg gagaaacgac ctgaccttct
    59761 gcagcttctt cacacgcttt cgcaataggc gcatcatcca gaccgatgat tgccgccata
    59821 gcgcccgtgc cttccggtac ggcttcttgc atgaacttac cgcgcatctc aaccagacgc
    59881 accgcatcag cgaaatcaat cacgccagcg caaaccagcg cggagtattc ccccaggctg
    59941 tgaccggcca tcattgccgg tgctttaccg ccctgctgct gccatacgcg atacagcgca
    60001 acagatgcag tcaacagcgc tggctgggtt tgccaggttt tattcagttc ttcagctgac
    60061 ccctgctggg tcagcgccca caggtcgtag cccagcgccg cagaagcttc agcaaacgtt
    60121 tcttcgacaa ttggatagct cgccgccata tcagccagca ttccaacggt ttgagaaccc
    60181 tgtccaggga acacaaatgc aaattgcgtc atgttttaat ccttatccta gaaacgaacc
    60241 agcgcagagc cccaagtgaa tccaccgcca aaggcttcaa gcagaaccaa ctgccccggc
    60301 ttaatgcgcc cgtcgcgtac agcttcatcc agcgcgcacg ggacagaggc cgcagaggta
    60361 ttaccgtggc gatccagcgt caccacgaca ttgtccatcg acataccgag ttttttcgcc
    60421 gttgcactga taatacgcag gttagcctga tgcggaacca gccagtccag ttgagaacgg
    60481 tcaagattat tcgccgccag cgtctcatca acgatgtgcg ccagttccgt taccgcaacc
    60541 ttgaagactt cgttgcccgc catcgtcaga tgaattgaat tctctggatt cacgcggtcg
    60601 gcgtttggca gcgtcagcaa ttcaccataa ctaccgtcgg catgcagatg ggtggaaatg
    60661 attcccggct cttcagaggc agccagcacc gcagcgcccg cgccatcgcc aaaaataata
    60721 atagtcccac gatctgttgg atcgcaggtg cgcgccagta catcggaacc gacgaccaga
    60781 gcatacttca ccgccccaga tttcacgtat tgatcggcta cgcttaatgc ataggtgaaa
    60841 cctgcacagg ctgctgcaac gtcaaatgcc gggcaacctt taatgtccaa catgctttga
    60901 atctgacaag ctgcgctcgg gaaagcgtgc gtagcagaag tcgttgccac aacgatcagg
    60961 ccaatctggt ctttctcaat gcccgccatc tcaattgcgc gtgtcgccgc ttcaaagccc
    61021 atggttgaaa cggtttcgtt tggcgcggca atgtggcgtt cgcggatacc ggtacgagtg
    61081 acaatccact cgtcagaggt gtccaccatt ttttccaaat cggcgtttgt ccgcacttgt
    61141 tcgggcagat agctgccagt accaataatc ttcgtataca tgtacgctca gtcacttttc
    61201 ggttatatac cgtcacttgc aaactgcgag ttcgctggca gcgtcctgct accgcagagt
    61261 tccgcttttg ccaccgtcca gcagctcaaa accagctggg tatacagatt ccaggcgagc
    61321 ggcaattcgc tgaggaactt gtcgctgcac cgcctgcact gcctgttcaa tcgcgaccgc
    61381 aaaagctcgc tgattggctg caccatgact ttttatcacc gtgccgcgca atcctaacag
    61441 acaggcgccg ttatactggt cggggttgag gtgactgaat cgcctcgtca ggctcttttg
    61501 tagccaacgc tttaataaca gtagccacca cgaccgtttt ttcccttcac cctgagattt
    61561 cagcagagaa aggaacatcc tgacaacacc ttccatcgtc tttaacgtga catttcctgt
    61621 aaagccgtca caaaccagca catctgtctt gccagttaac aactcattgg cttcaagata
    61681 gccgatataa ttgatagaag ggattgtttt aagcaccgct gaggcatccc gaatactgtc
    61741 aagacccttt acttcttctt caccaatatt gagcaacgcc acgcgaggat tgggaatttc
    61801 caccacctct tcagccagaa ctgagcccat aatggcaaat tgcaccaaca ttgtgctgtc
    61861 acaatcgacg ttggccccta aatcgaggac caccgttttg cccttttgct gatgtggtaa
    61921 taccgtcacc agcgccggac gctcaatccc ctccaggggc ttgagtaata attttgccag
    61981 ccccatcagt gccccggtat taccggcact gacacaggct tgcgctcgac cttctttcac
    62041 cagctccagg gccatacgca ttgaactccc acggctggcg cggatagctt gcgaaggccg
    62101 ggcatcactg gcgataactg actgcgcagg aataatctgc agacgcgaac gttgttcaaa
    62161 gtcagcttta gcaagtaatg gcgtgatggc gtcgggattg ccgactaaaa gaagagtgag
    62221 ttgcgaatta gaattcagtg cctgcaatgc tgcaggcact gtcacggaag ggccaaaatc
    62281 ccctcccatg acatctaacg ccagggttag acgtgtcaag gtatcgtcgc cgcccggttt
    62341 ggtctttccc cagttgcctg gggaaaatcc tcactaagct tcatcacgca gacgcgtgat
    62401 tacttagcga tgaccttgcg gccgcggtag taaccgtcgg cagtgatgtg gtgacgcagg
    62461 tgtttttcac cagaagtttt gtctacagac aggctggtga ctgcggtcag cgcgtcatgg
    62521 gaacgacgca tgccacgttt ggaacgggtt ggtttattct gttgtacggc catggacctt
    62581 actcctcaat tacggtttat ccccgatcca acggggagca ccaattactt acgctttaag
    62641 ctggctaata cggcaaatgg gtttggcttt tgcacttctt caggcagttc accaaagacc
    62701 atgtccgctt cggacacttc acagtgttca gaatcatgca ccggaactac cggcaaggcg
    62761 aggatgattt catcttcaac cattgcaagc agatcgattt caccgaattc gttaacctca
    62821 atcggttcat acgcttccgg cagtgcttca gcctgttcgt ctgaacgcac aggactaaaa
    62881 caatacgttg tgtagacctg atgagtaaac ggcttcccgc aacgctgaca ctcgagcgtt
    62941 accgtcacct tcgcatcgcc gtttaacact gcgagacgtt ggttatcgat agcgaacgac
    63001 atggagcatt ccacatcact gtccacactg actacggatt cggcgacgcg ctcaacctga
    63061 tcaggggtat agataccctg gtaatcaagg cgtttttgag ccgtacgaac cggatcgaga
    63121 gtcaggggta attttacctt ttgcataggg cgcgcatatt aactttgtaa cgtcatagag
    63181 tcaaagaaaa aggcagcctg tggttgcctt ttgccaataa ttcgcacaca ttgcgggtta
    63241 ctgctttatt ttcgttcagt gctgataacg aacatcaacc tgcaaaacga agcgcatagt
    63301 ttaaaatggc tatcatcaat acgctatatc tgatggaaaa aaatatgcct aaacttattt
    63361 tagcctccac ctcgccatgg cgtcgcgctc ttctggaaaa actgcaaatc tcttttgaat
    63421 gtgcagcacc tgaggtcgac gaaaccccac gcagcgacga atcaccgcga cagttggtgc
    63481 ttcgactggc acaagaaaaa tcgcaatctc tggcgtcacg ttatccggat catttaatta
    63541 ttggttcaga ccaggtatgt gttcttgatg gcgaaatcac cggtaaaccg ttaacggaag
    63601 aaaatgcccg tctgcaatta cgcaaagcca gcggcaatat cgtcaccttc tatactggac
    63661 tggcgttgtt taattcggcg aatgggcatc tacaaacaga agtggagcct tttgacgtcc
    63721 atttccgtca cctaagcgag gcggagattg ataattacgt gcgtaaggag catcccctgc
    63781 actgcgcggg tagctttaag agtgaaggat ttggcattac gctgtttgag cgtttagagg
    63841 gacgtgaccc taacacgctg gttggtttac cgctaatcgc gctgtgtcag atgttacgtc
    63901 gggaagggaa aaacccgctg atgggatagt tgtttgcgcc ggatgcggtt acgtcgcatc
    63961 cggcaatcgg tgcaggcggc ctgatgcggc gtaaacgcct tatcaggcca accgcccatt
    64021 atcaggctta tatgcttgta ttagcgcgcg ttacgcagct tttgcaaaca acgcttcaaa
    64081 ccttcatcca tcggcgcttc gatacgcatc acctcaccgg tccccggatg ggtaaacttc
    64141 aacgctgcgg cgtgcaggaa cagacgattt aatcccgtgc ctgcttcagt gagctgtctg
    64201 tcaaattcac ggtcaccgta gcgatcgtca aaggcaatcg gatgacccgc atactgtgta
    64261 tgcacacgga tctgatgagt acgtccggtt accgggctac aacgcaccag ggtggcaaat
    64321 gcatagcgtt cttccacttt aaagcgtgtt tccgacggtt tgccttcctg actgacacgc
    64381 acgatacgtt cgccgctttg cagaatattt ttcaataacg gcgcttgaac gctcttcaca
    64441 tgcgactgcc actgaccgcg caccagcgcc aggtaatctt tttgcattcc tttttcacgt
    64501 aattgctcat gcagagaacg caacgccgag cgttttttcg ctaccagcaa cacacctgaa
    64561 gtgtcccggt cgagacgatg aaccagttca aggaaccgcg cttccgggcg caacgcccgc
    64621 aaaccttcga taacgccgaa gcttaaacca ctgccgccat gtaccgccgt accggaaggt
    64681 ttattcagca ccaggatatg atcatcttca tataagatga cgtccgccag cgccgccacc
    64741 ttttgcagat gtggcgaaac cgcctcttct tcccgctcag caacgcgaac cggtgggata
    64801 cgcacctcat cacccgcttc gagtttatat tcaggcttaa tacgtttttt gttcacccgc
    64861 acttcacctt tacgcaaaat acggtaaatc atactttttg gtacgccttt caattgggta
    64921 cgcaaaaagt tatcgatacg ttgccccgct tcgtcagcgg tgatagcaac aatttttacg
    64981 gatggagtct ctgttttcat ggtgggcgat tctaattagc caacaggatt cgcgccactc
    65041 atttttctat gcttatattt agttttgcac cttattactt cactacgtga tcactttatt
    65101 gatggttatt aaaccaatca ccagcaagaa gtgaaaaaac tgtgagtaag cgggtgataa
    65161 atggtaaaag tcatcttgct ataacaaggc ttgcagtgga ataatgaggc cgtttccgtg
    65221 tccatccttg ttaaaacaag aaattttacg gaataaccca ttttgcccga ccgatcatcc
    65281 acgcagcaat ggcgtaagac gtattgatct ttcaggcagt tagcgggctg cgggttgcag
    65341 tccttaccgg tagatggaaa tatttctgga gagtaatacc cagtctgttt ctttgataat
    65401 tgcgctgttt ttccgcatga aaaacgggca accgacactc tgcgcctctt tgagctgacg
    65461 ataaccgtga ggttggcgac gcgactagac acgaggccat cggttcacac ccggaaaggc
    65521 gttactttgc ccgcagctta gtcgtcaatg taagaataat gagtaagtta cgatgaaaag
    65581 aatgttaatc aacgcaactc agcaggaaga gttgcgcgtt gcccttgtag atgggcagcg
    65641 tctgtatgac ctggatatcg aaagtccagg gcacgagcag aaaaaggcaa acatctacaa
    65701 aggtaaaatc acccgcattg aaccgagtct ggaagctgct tttgttgatt acggcgctga
    65761 acgtcacggt ttcctcccac taaaagaaat tgcccgcgaa tatttccctg ctaactacag
    65821 tgctcatggt cgtcccaaca ttaaagatgt gttgcgtgaa ggtcaggaag tcattgttca
    65881 gatcgataaa gaagagcgcg gcaacaaagg cgcggcatta accaccttta tcagtctggc
    65941 gggtagctat ctggttctga tgccaaataa cccgcgcgca ggtggcattt ctcgccgtat
    66001 cgaaggcgat gaccgtaccg aattaaaaga agcactggca agccttgaac tgccggaagg
    66061 catggggctt atcgtgcgca ccgctggcgt aggcaaatct gctgaggcgc tgcaatggga
    66121 tttaagcttc cgcctgaaac actgggaagc catcaaaaaa gccgctgaaa gccgcccggc
    66181 tccgttcctg attcatcagg agagcaacgt aatcgttcgc gccttccgcg attatttacg
    66241 tcaggatatc ggcgaaatcc ttatcgataa cccgaaagtc ctcgaactgg cacgtcagca
    66301 tatcgctgca ttaggtcgcc cggatttcag cagcaaaatc aaactgtaca ccggcgagat
    66361 cccgctgttc agccactacc agatcgagtc gcagatcgag tccgccttcc agcgtgaagt
    66421 ccgtctgcca tccggtggtt ccattgttat cgacagcacc gaagcgttaa cagccatcga
    66481 catcaactcc gcacgcgcga cccgcggcgg cgatatcgaa gaaactgcgt ttaacaccaa
    66541 cctcgaagcc gccgatgaga ttgctcgtca gctgcgcctg cgtgacctcg gcggcctgat
    66601 tgttatcgac ttcatcgaca tgacgccagt acgccaccag cgtgcggtag aaaaccgtct
    66661 gcgtgaagcg gtgcgtcagg accgtgcgcg tattcaaatc agccatattt ctcgctttgg
    66721 cctgctggaa atgtcccgcc agcgcctgag cccatcactg ggtgaatcca gccatcacgt
    66781 ctgcccgcgc tgctccggta ccggtaccgt gcgtgacaac gaatcgctgt cgctctctat
    66841 tctgcgtttg atcgaagaag aagcgctgaa agagaacacc caggaagttc acgccattgt
    66901 tcctgtgcca atcgcttctt atctgctgaa tgaaaaacgt tctgcggtga atgcgattga
    66961 aacgcgtcag gacggtgttc gctgcgtgat tgtgccaaac gatcagatgg aaaccccgca
    67021 ctaccacgtg ttgcgcgtgc gcaaagggga agaaacgccg accttaagct acatgctgcc
    67081 gaagctgcat gaagaagcga tggcgctgcc gtctgaagaa gagttcgctg aacgtaagcg
    67141 tcctgaacaa cctgcactgg caacctttgc catgccggat gtaccgccag cgccaacccc
    67201 agctgaacct gccgcgactg tcgtagcccc agcaccgaaa gctgcaacgg caacaccagc
    67261 agctcctgca caacctgggc tgttaagccg cttcttcggc gcactgaaag cgctgttcag
    67321 cggtggtgaa gaagccaaac cgaccgagca accaacaccg aaagcagaag cgaaaccgga
    67381 acgtcaacag gatcgtcgca aacctcgtca gagcaaccgc cgtgaccgta atgagcgccg
    67441 cgacacccgt agtgaacgta ctgaaggcag cgataatcgc gaagaaaacc gtcgtaatcg
    67501 tcgccaggca cagcagcaaa ctgccgagac gcgtgagagc cgtcagcagg ctgaggtaac
    67561 ggaaaaagca cgtaccaccg acgagcagca agcgccgcgt cgtgaacgta gccgccgccg
    67621 taacgatgat aaacgtcagg cgcaacaaga agcgaaggcg ctgaatgttg aagagcaatc
    67681 tgttcaggaa accgaacagg aagaacgtgt acgtccggtt cagccgcgtc gtaaacagcg
    67741 tcagctcaat cagaaagtgc gttacgagca aagcgtagcc gaagaagcgg tagtcgcacc
    67801 ggtggttgaa gaaactgccg ctgccgaacc aattgttcag gaagctccag ctccacgcac
    67861 agaactggtg aaagtcccgc tgccagtcgt agcgcaaact gcaccagaac agcaagaaga
    67921 gaacaatgct gataaccgtg acaacggtgg catgccgcgt cgttctcgcc gctcgcctcg
    67981 tcacctgcgc gtgagtggtc agcgtcgtcg tcgctatcgt gacgagcgtt atccaaccca
    68041 gtcgccaatg ccgttgaccg tagcgtgcgc gtctccggaa ctggcctctg gcaaagtctg
    68101 gatccgctat ccaattgtac gtccgcaaga tgtacaagtt gaagagcagc gcgaacagga
    68161 agaagtacaa gtgcagccga tggtgactga ggtccctgtc gccgccgctg tcgaacctgt
    68221 tgttagcgcg ccagttgttg aagaaatggc tgaagtcgta gaagcccccg taccggttgc
    68281 cgaaccgcaa ccggaagtgg ttgaaacgac gcatcctgaa gtgattgccg ccgcggtaac
    68341 tgaacagccg caggtgatta ccgagtctga tgttgccgta gcccaggaag tcgcagaaca
    68401 cgcagaaccg atggttgaac cgcaggaaga gacggcagat gcagatattg aagaagttgc
    68461 cgaaactgct gaggttgtgg ttgctgaacc tgaagttgtt gctcaacctg ccgcgccagt
    68521 cgtcgctgaa gtcgcagcag aagttgaaac ggtggccgcg gttgaaccag agatcaccgt
    68581 tgagcataac cacgctaccg cgccaatgac gcgcgctccg gcaccggaat atgttccgga
    68641 ggcaccgcgt cacagtgact ggcagcgccc tacttttgcc ttcgaaggta aaggtgccgc
    68701 aggtggtcat acggcaacgc atcatgcctc tgccgctcct gcgcgtccgc aacctgttga
    68761 gtaataatta gctcaaagta atcaagccct ggtaactgcc agggcttttt tatttcatgt
    68821 ttgaatcaac atattaaaat tcatcaaatg gcatccttgc taaccaacaa tgcaaaatag
    68881 gcaaaaaaaa tgcccgcccc ggcgatccca gagcgggcag acaaaacata cccagtttca
    68941 tgatatgttt taaagcgagc ttatttgttg agctggaaga gcgacaatcc ctgcatatcg
    69001 gtaaatgctt tatacgatgc ctgcaatgcc gtttgctgca tgatgtaaga tgaaatagtt
    69061 gcattccagt ccacatcaac cagatcgctc atctgctgcg tttgccctaa agcgcgatcg
    69121 ctacctaagg aatccagcga ctccagtttg ttcagctgcg tgcctaattc cgcgcgaacg
    69181 gtaaggacat tattcagcga gtttttcagt ccacggttgg ttttatccaa tgccgcagcg
    69241 gcgatttctt tatccgcttc gctatccgcg accggcgttt tcagggctgc gatggcgcta
    69301 tccagcatgg caaaaagatt ggtttcagaa gcgctaccgt ctggttccgc taccgcgtta
    69361 ctggtaatac tgtcgaaaat tttgtcgccc gtatgtccta tcaccatcga acgcgaagca
    69421 tcgacctgtt gcttaatact ttctgttccg ccaacataat caccgttagc ctcactaaac
    69481 ggcgcagtct ctgttttata tgtagcgccc attaccgtca gtggtgttcg ccagattcag
    69541 caactggtca cgaagcccct gaatatccgt agccagcgag gcccggtcat cgtcactcaa
    69601 ggtgccattg ctggcgtaga caattttttc ctgagcattc tggatagcag tggtgacctg
    69661 gctaagtaca ctctcttcca gtgacacttt ttgagtggcg aaagtacgtg ccagcgtgta
    69721 ctggctgttt tgcgcctgtg cctgggagag aacaacagct tgtgatgcag caatgggatc
    69781 gtctgaaggg ttaacgactc gcttacccgt cgacatctgc tcgccgtact tcatccattc
    69841 tgcctgagaa ttggtgatgc cgcgcatgtt ttgctggtac atcatctggg tactgaaacg
    69901 cattttatct ccccttagcg aatgttaatc agcgcatcaa aaatcgcgtt tgccgtctgc
    69961 agaacctgcg cattcgccag gtaatactgc tgaaaacgtt gcagatttcc gtactcctca
    70021 tcgagattga caccggaaat cgactgctgc tgattggaaa gctgcgtcac cacattacct
    70081 tgcgtggtgc tactggtttt caacgtcgcg gttttattac cgatatcact cactaacgag
    70141 gcataagcgt cgttaaagga tttcgcaccg cccaccgttt tactgttgct ttgcagatcc
    70201 agcagggcct gaccgttgcg gttgtcgcta tcacccgcat cttcttcgct cgccatcgcg
    70261 attttcgctt cgtcggtgat taatacatcc atgttgacga tggcgtcact tactggtttc
    70321 agcgtgaagc tgtcgttaac ggcaggcgtt cctgtaaacg tcaactccag accatcaaat
    70381 gccactttac cgttggcatc tggtgtcgcc gtaaaagtgg tattgctggc aaggcgggtg
    70441 acctgccact gattattatc gaacgagatt ttgtaatctg tcgccagtac cacggaggca
    70501 tcagttaccg tggcaccgat cgcaacgtca cctttgtttt tcgtgttttg cagaaccgcg
    70561 ggcttaccga tagtaaagaa atcttcacca gcatcgccgt tagcatcaaa tccggctttg
    70621 tgttggctgt tgaaagcctc ggcaaatgcc agcgccagtt gtccaagcgt attacgcgtc
    70681 tggtccagat cctgagaacg gaatgtcaga atgccgccca gcgacccggt attcagtaat
    70741 ttctccggga tctcaatatt gcctgccgtc ccatcaacgt aagcgacagt cgtacgagaa
    70801 gggtcggcgc tggaaggaac tgccgccagt tgccgcgccg tacttccctg aaccagtgag
    70861 taaccattgg catcgtgatg ttataagtac cgccatcctg aacgctgact tctacgccaa
    70921 caatctggtt taattcgctc accagttgat cgcgttgatc cagcagattg ttaggtgacg
    70981 cccctgcccc cacgcctgtc aggcgcgaga tttgatcgtt caggctggca atttgtttag
    71041 cgtaattgtt gatctgatca acgctggcac ctatcgcgat attgacctgt ttgtcctggt
    71101 cgcgcagata ttgatcggtg gttttaaact gattcaccaa cccttctgat ttcccaatca
    71161 gcgcctggcg cgccgccggg tcttccgcgt tactcaccag cgtttgcagg ctggtgaaga
    71221 aatcctgcat ctgtgttgcc agcgaagagg tactggtgga gagcatattg tcgattttcg
    71281 acatctgctc atagcgggcg gtcaggccgc tactttgcgt ctgcgcagca cgtaactggt
    71341 tggtaataaa cgtatcatac tcacgctgca caccagaaac gtagacgcca ttgccaaccc
    71401 agccgccagc gcccaacgtg ctattggcct gcgccataat agtggtttgg cgggtatatc
    71461 cggcaacgtt atagctggag atattattac ttgccgtatt taacgccgcc tgggccgcgt
    71521 tcagtccgct catggcgtta ttgatcaagc tggacatgga ggttccttat aagccttcaa
    71581 ttacagagta ttatcggcag cgacccgccg gacttgagtt attcagaaca gattatcaat
    71641 gttcatgctg taggttttgc tcaccttgtc gcttatcgat ttcatctgct ggatcatgtt
    71701 ggtgagttta cgggcatagt gaggatcggt agcatagccc gcgtcctgta ggacctgcgc
    71761 cccctgttcc gcactcgcgg cggtcgtcac ggcggcgtag cgcgggttac gtgttaacag
    71821 cccaacgtaa tccgacaatg cttccagata cgagctgtag acccgaaact tcgcttttac
    71881 tttcttcgct tcgccgtttt catattcagt cgtggtgatt tcagtgactt gccctttcca
    71941 gttgccagag gctttgacac caaacaggtt atagcttggc tcgccgtttt cgcggcggat
    72001 ttgccgttgc ccccaaccag attcaagcgc agcctgagcg aggatcaaat gatgtggcac
    72061 accgctttgc tggcttgcca gttgggcggg cagcgagagt tgcgcgagga atgctctact
    72121 gtcacccggc agcgaatcat cgtagttacg tggtacggcc ttttgcacca gctgcgaaag
    72181 cgtctggttt tgataacgca ccacggtttc gagcgggaat ttcatcggtg ctgctggcgt
    72241 ggactcctct ggcaatggtt gttctggcgt catctgttta accatcatct ctgcaagccc
    72301 cagacctttg cccgccgtca tctgttgggc aatttgctgg tcatacatac tggtatacag
    72361 acgagtgtgc tcgctgctga acaggccatc ttttggtaaa gcgtcgcgca tgcttttcaa
    72421 catcatctgc acgaacatcc cttccacctg acgggccacc ggacggatat ttgccgccgg
    72481 atcttcgctc gctttcgcct ttagttcgtt gagtgattgc gcatcccagg ccgcacttgc
    72541 cagtagtttg ctgtcgctga tcatcagatg atttccagtt ttgcccgcag acatcccgca
    72601 ctttgcattg attgcaaaat agacatcaga tccatcggcg tagcgcccag cgcattgagc
    72661 gcacgcacca cgttattgag gctggcgctg gaacgtacgc tttgcagcga accgccgctc
    72721 tggcgtaaat cgatctgcgt ttgtggagta acaacagtct gtccaccacc aaacggtgta
    72781 tctggctggc tgacattggc ctgacgatta actgttactg agagatttcc ctgcgctacc
    72841 gcgcagctgt cgagggtcac ttcgcgattc atcaccaccg aaccggtgcg cgagttaatc
    72901 actactttag cgtcctgcgg ggtgacatta acctgcatat tctggatatc ggcaaggaaa
    72961 cggacctggg aactgttgcc acttggtacg cgcacctgaa tagtccgcgc atctaacgcg
    73021 gtggcgctgc catatccacg cacgcggttg atggtgtcag cgatttgctg cgccatgctg
    73081 aaatcttcgt cgttaagttg caaattaagg gtattcccga cgccaaactg gctgggcaat
    73141 tcacgttcaa taaccgcacc attggtgatc cgtccaccgt tcagttggtt aacctgaaca
    73201 ctgctaccgc ctgcggaggc tcctgcgccg ccaaccagaa tattgccctg cgccagcgca
    73261 tacacctgac tgtcaacgcc cttaagcgga gtcatcaaca acgtacctcc acgcaagctt
    73321 ttggcattac ccatggaaga aaccaccaca tcgatggttt gcccctgacg tccaaacgga
    73381 ggaagtgacg ctgtcaccat taccgcagcg acgtttttta gctgcatatt ggtgcccgtc
    73441 ggaacggtaa ttcccagctg tgagagcatg ttattaagcg tttgtgtggt aaacggcgtc
    73501 tgggttgtct ggtcaccagt gccatccagc cccactacca agccatagcc aatcagtgag
    73561 ttttgcctta ccccctgaac actggtgaga tcgcgaatac gctcagcctg agccgccgtc
    73621 gtaaccagta gaagaattaa tgcagagaga aatttaatca ccacagcctc acttacattg
    73681 gcgacaggtt aaggaagaaa cgctgcaacc agcccatatt ttgcgcttcg ttaatgtagc
    73741 cattgcctac gtattcaata cgcgcatccg ccacctgagt agacggtacg gtattgctgc
    73801 cgctgatagt gcgtggatta accacgcccg agaagcgaat aaattcggta ccctgattaa
    73861 tggcaatctg tttttcaccc accacatgca ggttgccgtt gaccagtacc tggtcaaccg
    73921 tcaccgtcaa cgtgccgcta aaggtattgc tggcattggc cccgcccttt ccgttgaacg
    73981 tgttaccacc ggaggcttcg acatcggcac gagcgttacc aaacagcccc tgcaaatagc
    74041 gcggcacagt atcaaagcca aaattagttt taccgtcacg gctggcattc gcagaggagc
    74101 ttttgctggc gctgacgttc tcctgcaaca cgatggtcag cgtatcgcca atattgcgtg
    74161 gtcgacgatc ttcaaacagc ggttgatagc catagttaat cggctgagca gactggaaaa
    74221 tagaaccgtt ggcgacgggc gtcggaccgg gaaccggttg tgcactggtc gccccctgca
    74281 ccagcggcgt ggagggtatc caggcgcagc cggttagtga aagcaccaac aagctggaaa
    74341 tggcataagt atgcgcagcg tttttttgca tggctatcat cttcaaaaat cagtaaaccg
    74401 gtgaacctgc caccggttat gccttagagt tgcgtcagtt tttgcagcat ctgatcggtg
    74461 gtggacaccg ctttactgtt gatttcgtaa gcgcgttgca cctgaatcat attgaccagt
    74521 tcttccgcca cgttgacgtt agacgtttca acataccctt gatacagcag tcccgcgccg
    74581 ttcaggcccg gcgtgctttc gttcggtgca ccggaggatt gcgtttcggt gtagaggttt
    74641 tcgccaatgc tctccagtcc ggtatcattc ataaaggtgg tgagattgag ctgcccaacc
    74701 tgaaccggag ctgcctggcc ttgttgggtt acgctgacca cgccatcacg accgatggtg
    74761 atacttaacg cattcgccgg aatggtgatc gctggctgca cctgaaaacc accagccgtc
    74821 accagctgcc cgttctgatc cacctggaaa gagccgtcac ggatataggc tgatgagcca
    74881 tctggcaaca ttacctggaa aaagccctgc cctttaatcg cgacatcttt gctgttattg
    74941 gtctgcgaca gattgccctg gctgtgtaag cgttcagtgg cgaccgggcg tacccccgtg
    75001 ccgatttgta atccggaggg taaggtggtt tgttcggaag actgtgcccc cggctggcga
    75061 atggtttgat aaagcagatc ttcaaacacc gcgcgctgac gcttaaaacc gttagtactg
    75121 acgtttgcca ggttgttggc aatgacgtcc atattggttt gctgggcgtc aaggcccgtt
    75181 ttggcgatcc ataatgaact gatcatataa tgtcctgtag tggattgctg acaagcagag
    75241 cagaagcacg cgacagtgtg aatttcccct caccctaacc ctctccccaa aggggcgagg
    75301 gaatagtccg cgcgctttta ttgcaatgtc atcaatcgtt caattaactc atcgacagca
    75361 gttggttggc acggcctgcg ttatcatcga cgctgctgat caccttcatc tgcatttcaa
    75421 aacgccgcgc gctggcaatc atgtcgctca ttgccgcaac ggcattgacg ttactgcctt
    75481 ccagaacccc cgacatcaca cgcaaggttg gatctgcctg cagtaccggc cctcgcgtgg
    75541 cctgggtttc tgcgcttaaa cgaaaaatgc cgtcgtcacc gcgctgcact tcgctgcccg
    75601 tggctttcac cagtttcaga cgccctactg gcgcaactgt atttgccgga tcgcccggat
    75661 tgagcgccga gattgtgcca tcggcagcaa tcgtgatttc cgccccttcc ggcacagcaa
    75721 ttggccccgc ctcgcctatc accggatgcc cctgaattgt cagttgcccg gtgggatcaa
    75781 cctgaatgct gccattacgc gtataccctt cgctgccgtc agcggtctgc acggccagcc
    75841 atcctgctgc aacgcaacgt ccagcgggcg agaggtgtaa tccatcttgc cgggcgtcat
    75901 gtctgcaccc ggtgttgacg ccgttaccag cgtgcgcgtg ggcagtgaaa gcccttccac
    75961 cggcactgcg cgcaaggcgt ttaactgcgc gcgaaaaccg ggcgtagagg cattggccag
    76021 attactggcg gttaccgcct gttgattcag cgtctggctg gctgctccca tcgcagtata
    76081 aattgcgtga tccattgagc tatcccgtca gcgattagcg taagttaacc cgcgtgttga
    76141 ggatctggtc ctgggttttg atggtctggg cgttagactt atagttacgc tgggcaacga
    76201 tcatattgac cagttcttta ctgagatcga cgttggacgc ttccagcgca ccgttggtca
    76261 gagtgccaaa gtttcccgtc ccggctgtcc ccaacagcgc cacgccagaa gattgcgtcg
    76321 cagaccagac gttgtcgcct tcggatgcca gaccttcgtt gttggcaaag ttcgccagta
    76381 caatctgccc cagcagttgg gtttgttcgt tggaattgtt gccgacaacc gtaccgtcat
    76441 cattgatttg ataactcacc agatcgcccg gtttgtagcc attctgggtg gttgccacaa
    76501 tgttgttagc gccggtattt tgctgcatgg agttgaggaa gctcagacta aacgtggcgg
    76561 gttctgcacc gttaattgcg ccggttgcga tattattcgc catcgcacca tccactaatg
    76621 tgccattagc attaaattcc agtgttgtcg ctgtcttcgc aatgctgttt ggatcactgc
    76681 tatcctgggt gtagacctgc cagttattat ccccggtctt cacaaaatag acgctcatgt
    76741 catgagcatt accctgactg tcgaaaacag tcaccgaacc ttttttgtta tagctatccg
    76801 cattgctggc gctgaatggc gtaacagtag gaagcggatc actggaattc aggttgatct
    76861 gcatcgacgc cgtggtggta gttttcgctg ccatcagggt attcgggatc gaaatattgg
    76921 tcggattcgc cccttgctga atagtcggcg gcgtaccggt taccgggtaa cccgtcagct
    76981 gtaaaccttg cgtattcaac aggttacggt tttcatccag cttaaattgt ccgttacggc
    77041 tgtagaacac cgaaccgttg ctgtctacca gacggaaaaa accgttctgg ctgatagcaa
    77101 cgtccagacc tcgcccggtg ttggtggtcg tgccatcggt aaagtcctga gtgataccgg
    77161 caacttttac ccccagtccc actttcgaac cggcaaacat atcggcaaaa gaggccgtgc
    77221 ctgatttaaa gccgtaggtg gcggagttgg cgatattgtt gccaataaca tcgaggttgg
    77281 tggcggcagc gtttaatccg ctaaccgctt gagaaaaggc catgactgac tcctgaaatg
    77341 tgaaggctta aattatctgc cgtacttcgt cgagggtggt ggtgccgtaa gtgccgagat
    77401 ccagcgtatt accgctgttg ccgcggatca caccctgcac cagagcaaac tgcagcggct
    77461 gggcaaccag ttgtgtacca ccgttactgg cgctaatcgc tacattgtaa gaaccgttcg
    77521 gcgcagtgct gccatcagtc aacgaaccgt cccaggtgaa actgtgaact ccggcggtca
    77581 gttcaccaat atcaatggtg cgcacaaccg cgccattttt atcggtgatg gtggccgtaa
    77641 ctttgtctgc cgcctgttgc agctcaacac caaacggcgt ggtcgtggtc acagcccctt
    77701 cttcactgcc ggttcccgca agaacagttg tgccggggat catcacgccg tgaccgatca
    77761 ggttactggc ctgtaacgac tggctgttat caatctgtcc ggaaatagat ccgagcgtgg
    77821 tattgagttt ttcaatcccg ctgaccgtgc tgatttgtgc caattgcgac gtcagctcgt
    77881 tgttttccat tggattggtc gggtcctggt ttttcagctg cgccaccagc aaagtcagaa
    77941 aactgctttg taaatctgcg gcgttgctgc ccgtgagcga actactgctg gtggtactga
    78001 cgccggtatt tgtcggatcg gtggtggtta ccgcaatgga catagctttc tcctttattg
    78061 accgagcgta agggttttca gcatcatgct tttcaccgtg ttgagcactt caacattggc
    78121 ctgatagctg cgtgacgccg acatggtgtt aaccatctcc ccgacgacat caacgttcgg
    78181 catttttatg tagccctttg catctgccag cggattacct ggttcataaa ccagtttgtc
    78241 cggggcctga ctttcttaac atcggcaacc tttacgccgc ctgtcgcagc acctggtgca
    78301 gcgttaacct ggaataccac ctgttttgcc cgatatggct gtccatcagg accggtcacg
    78361 ctatcagcat ttgccagatt actggccgcc acgttcaggc gctgggactg ggcagttaac
    78421 gccgacccgg cgatatcaaa aatattcagc agtgccatcc gttaatttcc gctctgtaaa
    78481 acgttcatca tgcctttgat ttgcccgctc aacgcgctaa ggctcatctg gtattgcagg
    78541 ctgttatcgg caaactgggt gcgttcgcga tccatatcga cggtattacc gtcaagcgaa
    78601 ggctggtccg gaatacggta ttgcagttct gcggtaggag gcgtcagcgc ctgcgccggg
    78661 atgtgctgcg ttgaggtcat cgtcagtgca accacactgg ttgcatcccg tccacgttgc
    78721 atgacttttt taagttcact ggcaaaatcg atatcgcgcg cctgataacc aggggtatcg
    78781 gcattggcga tgtttgctgc cagcacttcc tgacgctggg cgcgcagatt gagcgcctct
    78841 tgttgaaaac gtaaggcggc gtcgagctta tcgagcatat ctcctccgca ggtatcaaaa
    78901 ttctgccatc agcttaaatg cctttacgcg cgcgttatcg gcggaataaa cgcaaaatgg
    78961 gtcgctattt atgccgttga tggtcattgc ggacaggtac aattcacgtt gtagaaatgg
    79021 ctgggggcga aaatgctgac aataaaacgt agcgtggcga tcatcgcgat actgttcagt
    79081 ccgttaagta cggcgagcaa tctcacatcg caattgcaca acttttttag cgcccaactc
    79141 gcgggggtaa gtgatgaggt tcgtgtttct attcgtacag cgcccaatct actaccgcca
    79201 tgcgagcagc cattgctttc gatgagcaat aattcccgcc tgtggggcaa tgtgaatgtg
    79261 ttggcacgct gcggtaacga caaacgatat ttacaggtta atgtgcaggc cacaggaaat
    79321 tatgtggttg ccgcgatgcc tattgtgcgg ggaggaaagc tggaagctgg caatgtcaaa
    79381 ctgaaacgtg gacgcctgga taccctgcca ccgcgtacgg tgctggatat caatcaactt
    79441 gttgatgccg ttagcctgcg cgatctatca cccgatcaac ctatccagtt aacccagttt
    79501 cgccaggcat ggcgggtaaa agcgggacaa cgcgtcaatg tgatcgccag cggtgatggg
    79561 tttagcgcca acgcagaagg tcaggcgctg aacaatgcag ccgtcgcaca gaatgcgcgg
    79621 gtgcgcatgg tatcggaaca ggtagtcagc ggcgttgttg atgcagatgg gaatattctt
    79681 ataaacctgt aagctgttaa agattacccg tcccttgccg ataaataagc aacacatgat
    79741 aaaagcgccc tcaatgagga ataaaccatg agtattgatc gcacttcgcc tctgaagcct
    79801 gtaagcaccg ttcaaccgcg cgaaaccacc gacgcgccgg taacgaacag ccgggcggca
    79861 aaaacaaccg cctccaccag caccagtgtg acgttaagcg acgcgcaagc aaaactgatg
    79921 caacccggca gcagtgatat caatcttgaa cgtgtcgaag cgttaaaact ggcgattcgt
    79981 aacggtgaac taaaaatgga caccggcaaa attgccgatg cgctgatcaa cgaagcgcag
    80041 caagacttgc agagtaactg atcgtatgac acgtcttgca gagatcctcg accagatgtc
    80101 cgctgtgctt aacgatctca aaacggtaat ggatcaagag cagcaacatc tctctatggg
    80161 gcagatcaac ggcagccagt tgcaatggat tacagaacaa aaaagctcac tgctggcgac
    80221 gctggattac ctcgaacagt tacgcaagaa agaacccaat acagcaaata gcgttgatat
    80281 tagtcaacgc tggcaggaaa ttactgtgaa aacgcagcaa ctacgccaaa tgaatcaaca
    80341 taacggctgg ttactggaag gacagattga gcgcaatcaa caggcgctgg aaatgttgaa
    80401 accgcatcag gaaccgacgc tatatggggc gaacggtcag acctcaacac cccatcgcgg
    80461 cagtaaaaag atttcgatct gaatattatc tccggcctgc actgcaggcc ggaatgcatt
    80521 gttacaccgt ccggcgggca aattctttaa ctttgaagcc cagtaccgcc agtgcagcga
    80581 agtacgcggc aatccccgcc agcacgaccg ccattaaacg cagtaagcgc cagggcatgg
    80641 tacccaatga ccactccggc atgatatgta acatacctaa aagcacgcca gacatcacca
    80701 gtaccgccac caccagacgc aacagaaacg ccatccagcc gggttgcgga gtaaagattt
    80761 tctgcttacg caactgccag taaagcagcg aagcattcag acacgccgcc agaccaatag
    80821 aaagtgacag cccggcatgt ttcaacggac caataaacgc caggttcatc aattgcgtta
    80881 aaatcagcgt aacgatggca attttcactg gcgttttaat gtcctggcgg gaataaaagc
    80941 caggagccaa cactttcact acaatcaggc caatcaaacc caccgagtag gcaattaacg
    81001 cccgctgggt catcagcgca tcaaacgcgg taaatttacc gtactggaac agcgaaacgg
    81061 ttaacggacc tgaaagaatg cccaacgcaa ccgcactcgg cagtgccaac aggaaacaaa
    81121 gacgcaaccc ccagtccatc aaacggttgt attcatcatg attgccactg gcaaaacttt
    81181 tcgacagcga cggcagcaaa atggtgccaa gcgccacgcc cagcacaccg gagggaaact
    81241 ccattaagcg gtcggcgtaa tacatccaag acaccgaacc ggaagcaaga aacgaggcaa
    81301 aaatggtgtt gatgattaag gagatctggc tcacagagac gccaaggatc gccggtccca
    81361 tctgtttcac cacgcgcatt gctccagcat cgtggaagtt aatgcgcggc aggaccagca
    81421 tgccgatctt cttcaggtgc ggtagctgat acaccagctg cagaatgccg ccgaccgtaa
    81481 cagcccacgc cagcgccagc accggtgggt taaagtacgg tgcggcaaac agcgcgaaac
    81541 caatcatgct gatgttaagc agtgttggcg caaacgccgg aatcgagaag cggttccacg
    81601 tattcagaat cgctcccacc agcgacgcca gggagatcag caagatatag ggaaaggtaa
    81661 tctttagtag ctggctggtc agggcaaatt tgtcagctgt gtcagcgaag cctggcgcgg
    81721 tcaccatgat cacccacggt gcggcgagca tgccagcgac cgtcacaacc gccagcgcaa
    81781 gtgtcagcag gccagaaaca taagagacaa agacccgcgt ggcgtcttca ccctgcttac
    81841 ttttatattc cgccagaatc ggtacaaatg cctgggaaaa tgccccttcg gcaaagatac
    81901 ggcgtaacaa gttaggaagt ttaaaagcga cgaaaaaggc gtcggttgcc atccctgcgc
    81961 caaagattct ggcgacaatt gcgtctcgtg cgaagccaag cacacgcgaa aacatggtca
    82021 tcgagctgac ggcggccagc gattttaata aattcatcgg tgttctaatc cggacccacg
    82081 ggcaaaacgc ctgcaaggca ggcgctgaaa gaggcgaata gtctacctag attatggtga
    82141 attgctaccg ccagatgtta cagggtttat tcactcatcg catcgcgcca gatcttgtca
    82201 acgatacgtt gcgccagcac tgcctattcg ccggcggttt gcggaactgt ctgattttac
    82261 acgcattcaa tgaagtgccg cgcacagccg acaaatccac gttgctcaag agtgctctgc
    82321 caaccaggaa tcggtttatt cactacaccc tgcccgcgct cctcacgcca ttcgcgcata
    82381 tccgtaatgt cgatgagcgc accgtcagtc acggcctgca cggtttcacg ctgacttccg
    82441 gcacggcgat gcatacaagt ggtgatctgc aaaggaccgg ccgaaaaatg gtgctcggca
    82501 aacagcattt cgccagcgtc gttagtcagt agcgtaccgc catccagaga ggctttgccg
    82561 cccgacaacc acagcgcggt atccaccaca tgcagataat catccagcaa cgtgaaataa
    82621 agatcgtgtg gcccgacgct attgctacga tgtttatcca ttcttagcga ggctgcggtg
    82681 gcgagttgcg tttttaactc accgtagagt ggtgcgaaac gacggttaaa accgaccatc
    82741 aacgtcagtt ttttccgcgc cgccagttcc accagccttt cagcatcgcg cagattttct
    82801 gccagcggtt tatcgacaca gacatgtacc cccgcattga gtaacgtact gaccacgtca
    82861 aagtggctgg cggtgctgga gtgcacaaaa accgcatcac aactggcggc aaggctggat
    82921 aacgaatcgg cataaggaat gcgccagctg tcacaaattg gcagggcttt ggcgcgcgta
    82981 ggcgaccagg ctccttgtaa cgtccagtca gacgctgccg ccagcaccgg taaccacgct
    83041 ttttgcgcaa tgccgcctaa tcccactacg ccgatacgta attttttcac tttaatctcc
    83101 cagatgggcc agcaacgaat caagacgttg tttcagttct gccacttcga tttccagggc
    83161 ttcgacgcgg gtctgtaaat caccgtcaac catgttcgac atagccgtta ccgccggctg
    83221 atcttcaacc tcaccactga aaagatgcat gtagcggctt tcgcgtttac ccggttcgcg
    83281 ggccagacgc accacaaaag gaccatcttc gcgattcgcc agttgttcca gcgtcgactc
    83341 cacttccgcc atatcgctga attcatacat tcgcgcggcg cggctgcgca gttcacccgg
    83401 cgtctgggca ccacgcaata acaacgtggt gattaacgcc acttctgcgg cgctcagttt
    83461 cagatcgcca aattctgaat tacaaaaacg ttgctcatat ttggtgaccc gattaccaaa
    83521 accgctcact gtgcgtagat aatgacgttt gaccagatta tccagctgtt cctgcacttc
    83581 ggattcactc agattcatga ccggttcacg gttcgttttc tgattacagg ccgtgactac
    83641 accattgact gagagcgggt attgctccgg cgtggtcacc tgcttttcca gcaagcaacc
    83701 aattacgcga gcttccaggg cagtaagttg atatttcatg agattctcct tagcggccgg
    83761 gcgtccagtc tggggtagtt aatgccgtca gtacgtgatc gcgccattgt ccatcaatca
    83821 acagatagtc tttcgcatag ccttcttttt caaaacccag tcgcgccagt aaatcaccgc
    83881 tgcgtttatt gtgcggcata taattagcca taatgcgatg aatatgttgg gtgcgctgca
    83941 tataacgaat ggctgcggtc agggcttcaa acatgagtcc tttgccctgc catttttgcc
    84001 caatcgaata accgagatag caggcatgaa aagagccgcg aacaacattg gaaaaattgg
    84061 caacgccaat aatctctttt tcgtccgggt cgaataagcc aaagtagaaa gctgaacctt
    84121 gtttatgaaa ttcgttaatc atccctagcc tggcctgcca gcctgatgga taacagtggc
    84181 tttcgtcgcg cactggctcc cagggcttga ggaaatggcg attctctgcg taataatccg
    84241 ccagacgcca ggcatcacga tcatgcacca gacgcacgac cagtcggtct gtggttaagc
    84301 gcacttttgg cacgttactg cgatagccaa acattctata cctactcctt cccgtaacgt
    84361 ctttaatacg ctgtaagtat tactatacct gcgcaaccag accctgtgaa aagtccaaaa
    84421 tgccattttc tgcattattc acatatttga taaaaactct ctatcaaagc gacgttttga
    84481 taaaaaaata ctgtccccga agtgatggga aaagtcttag cggcaatgca gaatagaaag
    84541 tgctcatctt ttatttcccg ggaggggaaa tgtcccgcgt atcgcaggcg aggaacctgg
    84601 gtaaatattt cctgctcatc gataatatgc tggtcgtgct ggggttcttt gttgtcttcc
    84661 cgctgatctc tatccgcttc gttgatcaaa tgggctgggc cgccgtcatg gtcggtattg
    84721 ctctcggtct acgccagttt attcagcaag gtctgggtat tttcggcggc gcaattgccg
    84781 accgctttgg tgccaaaccg atgattgtta ccggtatgct gatgcgcgcc gccggattcg
    84841 ccacaatggg tatcgcccac gaaccgtggc tattgtggtt ttcatgcctg ctctcgggac
    84901 tcggcggcac gttgtttgat ccgccgcgtt cggcgctggt ggtgaaacta atccgtccac
    84961 aacaacgtgg tcgttttttc tcgctgttga tgatgcagga cagtgccagt gcggtcattg
    85021 gcgcattgtt ggggagctgg ctgttgcaat acgactttcg cctggtctgc gccacagggg
    85081 ccgttttgtt tgtgctatgt gcggcgttca atgcgtggtt gttaccggca tggaaactct
    85141 ccaccgtacg cacgcccgtt cgcgaaggca tgacccgcgt gatgcgtgac aagcgttttg
    85201 tcacctatgt cctgacgctg gcgggttact acatgctggc tgtacaagtg atgctgatgc
    85261 tgccaattat ggtcaacgac gtggctggcg cgccctctgc cgttaaatgg atgtatgcca
    85321 ttgaagcgtg tctgtcgtta acgttgctct accctatcgc ccgctggagt gaaaagcatt
    85381 ttcgtctgga acaccggttg atggctgggc tgttgataat gtcattaagc atgatgccgg
    85441 tgggcatggt cagcggccta caacaacttt tcaccctgat ttgtctgttt tatatcgggt
    85501 cgatcattgc cgagcctgcg cgtgaaacct taagtgcttc gctggcggac gcaagagctc
    85561 gcggcagcta tatggggttt agccgtctgg gtctggcgat tggcggcgct attggttata
    85621 tcggtggtgg ctggctgttt gacctgggca aatcggcgca ccagccagag cttccgtgga
    85681 tgatgctggg aattattggc atcttcactt tccttgcgct gggttggcag tttagtcaga
    85741 aacgcgccgc gcgtcgtttg ctggaacgcg acgcctgatt tgatactccc ccctgactcc
    85801 tccatactga aagtgaactg acagccaaat ggaggagtca cgtgaagcta tacatttacg
    85861 atcactgccc ttactgcctc aaagcccgca tgattttcgg cctgaaaaat atccccgtcg
    85921 aattacatgt tctgctcaac gacgacgcag aaacacccac ccggatggtc ggtcaaaaac
    85981 aggttcccat tctgcaaaaa gatgacagcc gctatatgcc agaaagcatg gatatcgttc
    86041 actatgtcga taaactcgac ggcaaaccgt tactgaccgg caaacgttcc cctgccattg
    86101 aagagtggct gcgcaaggtc aatggctacg tcaacaaact gctgttgccg cgttttgcca
    86161 aatcggcatt tgatgagttt tctactcccg ccgcgcgcaa atatttcgtc gacaagaaag
    86221 aggccagcgc gggtaatttt gccgacctgc tggcccactc tgacggtctg attaagaata
    86281 tcagcgatga tttgcgtgcg ctggacaaac tgatcgtcaa accgaacgcc gtgaatggcg
    86341 aactttcgga agatgatatt cagctatttc cgctactgcg taatctgacg ctggtagccg
    86401 gaatcaactg gccaagccgc gttgctaatt accgcgataa tatggcgaaa cagacacaaa
    86461 tcaatttgtt atcatcaatg gcgatttaat cctgacccgc cgcggcagcc tgtattcgcg
    86521 ggttgccgcc gttttcagtt ttttctgctg gcgagtttat cgctggtgac acatgctgcg
    86581 ttgtgtcagg atattgacgc agatttcatg tcgaggacta ccatgaacaa gtttttattt
    86641 gccgctgcat tgatcgtcag tggcctgctc gttggctgta atcaactcac ccaatacacc
    86701 atcaccgaac aagaaattaa ccagtcgctt gcgaaacata ataatttctc aaaagatatc
    86761 ggtttacccg gcgtggctga cgcccatatt gttctgacaa acttgaccag ccaaattggt
    86821 cgcgaagagc cgaataaggt taccctaacc ggagacgcca atctggacat gaactccctg
    86881 ttcggtagtc agaaagcgac catgaaactg aagctgaaag cgctgcccgt gtttgataaa
    86941 gaaaaaggtg cgatcttcct gaaagagatg gaagtggtcg atgcgacggt acaaccggaa
    87001 aaaatgcaaa cggtgatgca aacgttgctt ccctatttga accaggcatt acgcaattac
    87061 tttaaccagc aacctgctta cgtcctgcgc gaagatggca gtcagggcga agcaatggcg
    87121 aaaaaactgg cgaaaggcat tgaagtgaag ccaggcgaaa ttgtcattcc gtttacggat
    87181 taatgacgag ggcgcattcg cgccctttat ttttcgtgca aaggaaaacg tttccgctta
    87241 tcctttgtgt ccggcaaaaa catcccttca gccggagcat agagattaat gactgcacca
    87301 tcccaggtat taaagatccg ccgcccagac gactggcacc ttcacctccg cgatggcgac
    87361 atgttaaaaa ctgtcgtgcc gtataccagc gaaatttatg gacgggctat cgtaatgccc
    87421 aatctggctc cgcccgtgac cacagttgag gctgccgtgg cgtatcgcca acgtattctt
    87481 gacgccgtac ctgccgggca cgatttcacc ccattgatga cctgttattt aacagattcg
    87541 ctggatccta atgagctgga gcgcggattt aatgaagccg tgttcaccgc tgcaaaactt
    87601 tacccggcaa acgcaaccac taactccagc cacggcgtga cgtcaattga cgcaatcatg
    87661 ccggtacttg agcgcatgga aaaaatcggt atgccgctac tggtgcatgg tgaagtgaca
    87721 catgcagata tcgacatttt tgatcgtgaa gcgcgcttta tagaaagcgt gatggaacct
    87781 ctgcgccagc gcctgactgc gctgaaagtc gtttttgagc acatcaccac caaagatgct
    87841 gccgactatg tccgtgacgg aaatgaacgg ctggctgcca ccatcactcc gcagcatctg
    87901 atgtttaacc gcaaccatat gctggttggt ggcgtgcgtc cgcacctgta ttgtttaccc
    87961 atcctcaaac gcaatattca ccaacaggca ttgcgtgaac tggtcgccag cggttttaat
    88021 cgagtattcc tcggtacgga ttctgcgcca catgcacgtc atcgcaaaga gagcagttgc
    88081 ggctgcgcgg gctgcttcaa cgccccaacc gcgctgggca gttacgctac cgtctttgaa
    88141 gagatgaatg ctctgcagca ctttgaagca ttctgttctg taaacggccc gcagttctat
    88201 ggcttgccgg tcaacgacac attcatcgaa ctggtacgtg aagagcaaca ggttgctgaa
    88261 agcatcgcac tgactgatga caccctggtg ccattcctcg ccggggaaac ggtacgctgg
    88321 tccgttaaac aataaaaatc gtagcttcct gttgtcatta ggttatttta cctgtataaa
    88381 taaccagtat attcaacagg gggctattat gcgaattgaa gtcaccatag cgaaaacttc
    88441 tccattgcca gctggggcta ttgacgccct ggctggcgaa ctttcccgcc gtattcagta
    88501 tgcgtttcct gataatgaag gccacgtatc ggtacgttat gccgcagcga ataatttatc
    88561 ggttattggc gcaacaaaag aagataaaca gcgcattagc gaaattctcc aggaaacgtg
    88621 ggaaagcgcc gatgactggt ttgtcagcga ataatatgca gtgatttttg tttttgccgg
    88681 atcgccccgg cttttttatt ttacttacga attttgagta caaaatttct acacttcctc
    88741 aaaataattg cacaatatgc taaaaaaata tgaatgacat gctgttttat tgttcatata
    88801 aatcttaaaa gcagaaattt atctgttccc ccctttaaaa attcggtgaa tacccttact
    88861 tattggtata ctgaagttgc ttcagataaa aacgcattga acctcgaata acgttgtcta
    88921 gtaacacgaa ttagggggcc atgatggaaa aaaataatga agtcattcag actcatccgc
    88981 tcgtagggtg ggacatcagc accgttgata gctatgatgc gctgatgttg cgtttgcact
    89041 accagacccc aaataagtcc gagcaggaag ggactgaagt tggtcagacg ctctggttaa
    89101 ccactgatgt tgccagacaa tttatttcga tattagaagc aggaatcgcc aaaattgaat
    89161 ccggtgattt cccggtaaac gagtatcggc gtcattaaca tgacatgacc atccacccaa
    89221 aaggcacctc accggtgcct ttaccatttc tgggttgtat atctgcattc agttaattac
    89281 tctgcttaaa ccatgagagc agagagaaaa aaatgaaata cgatctcatc attattggca
    89341 gcggttccgt aggcgctgcc gccgggtatt atgcaacccg cgccggttta aacgtgctaa
    89401 tgaccgacgc ccatatgcca ccgcatcaac acggcagcca ccacggcgat acgcgattaa
    89461 ttcgccatgc ttatggtgaa ggcgaaaagt atgtcccgtt ggtcctccgc gcgcaaacgc
    89521 tgtgggatga actttcccgc cacaacgaag atgatcccat ttttgtacgc tctggtgtca
    89581 ttaacctcgg cccggctgac tccgcatttc tcgccaacgt cgcccacagc gccgaacagt
    89641 ggcaactcaa cgttgaaaag ctcgatgcgc aagggattat ggcccgctgg ccagaaatac
    89701 gcgtcccgga caactacatc ggcttatttg agactgattc cggttttttg cgcagcgaac
    89761 tggcgattaa aacctggatc caactggcga aggaagcggg ctgtgcgcaa ctgttcaact
    89821 gcccggtcac cgcaattcgt catgacgatg atggcgtaac tattgaaacg gttgacggtg
    89881 agtatcaggc gaaaaaagcg attgtctgcg cgggaacatg ggtaaaagac ctgctcccgg
    89941 agctgcctgt ccagcccgta cgtaaagtat ttgcctggta tcaggccgat ggccgctata
    90001 gcgtgaagaa taaattcccg gcgtttaccg gtgaactgcc caatggcgat caatattatg
    90061 gttttccggc agaaaacgac gcgttgaaga ttggcaaaca taacggaggc caggttatcc
    90121 attcagcgga tgaacgtgtt ccgtttgcgg aagtggccag tgatgggtca gaagccttcc
    90181 cgttcttgcg caatgtattg ccgggtatcg gttgctgcct gtacggcgct gcctgcacct
    90241 atgataattc gcctgacgaa gattttatta tcgataccct acccggccac gataatacac
    90301 tgctcattac cggcctgagt gggcacggtt ttaaatttgc gtcagtttta ggggaaatag
    90361 ctgccgattt tgcgcaagac caaaaaagcg attttgattt gacgccattc aggctttccc
    90421 gcttccaata atcatcatta cggcctccag aatgaggccg tttttacagg agtatttaat
    90481 gcgtcggctg ttgcactatc tcatcaataa tattcgcgag catctgatgc tttatctttt
    90541 tctctgggga ttgttggcaa ttatggatct gatctatgtg ttttattttt agttttccgc
    90601 actgacataa actgacaaat atttaatctc cctttcattt agcgtgaaaa attgaatgag
    90661 attattcctg cttctacagc actcaccaga catctctgat gctgaaccca ctaaacttat
    90721 gaagattctt tcattattaa ttcaaaatta ttgaaataaa aataaatatc attcaatttg
    90781 ttatttttgt gttgcaattc attctccgac acgccatttt ctttctctcg caaattcgta
    90841 ttggatgcac ttatgtcatt cacaaatacc cctgaacgct atggtgttat atctgcggcc
    90901 ttccattggc taagcgcaat tattgtctac ggcatgtttg ctttaggtct gtggatggcc
    90961 acgctcagtt attacgatgg ctggtatcac aaagcacccg aactgcataa aagcatcggt
    91021 attttgttaa tgatggggct ggttattcgc gttctgtggc gtgtcatatc tccaccgccg
    91081 ggtccgctgc cgagttattc gcccatgact cgtcttgccg caaaagctgg acatctcgca
    91141 ttgtgcctat tgctgttcgc catcggcatc agcggctatc tgatctcaac tgccgatggt
    91201 aaaccgatca gcgtttttgg ctggtttgac gtccccgcga cccttgccga cgctggcgca
    91261 caggcagact ttgccggtgc cctgcatttt tggctcgcgt ggagcgtcgt agtactgtct
    91321 gttatgcacg gatttatggc cctgaagcat catttcatcg ataaagacga cactctgaag
    91381 cgcatgctgg gaaagtcgtc atctgactat ggagtataaa aaatgaaaaa aagcctgctt
    91441 ggtttaacct tcgcgtccct gatgttctct gccggttcag cggttgccgc cgattacaaa
    91501 attgacaaag aaggtcagca cgcctttgtt aacttccgca tacagcatct tggctatagc
    91561 tggttatatg gcacctttaa agatttcgac ggtactttta cctttgatga aaaaaatccg
    91621 gctgccgata aagtgaatgt gacaattaac accaccagcg tcgatactaa ccacgccgaa
    91681 cgcgataaac atcttcgcag tgcggatttt ctcaatacca caaaatatcc acaggcaaca
    91741 ttcacctcca ccagcgtgaa gaaagacggt gacgaactgg atattaccgg cgatctgacg
    91801 ctgaatggcg taaccaaacc cgtcacgctg gaagcgaaat taattggtca gggcgacgac
    91861 ccatggggcg gtaaacgtgc aggctttgag gccgaaggca aaattaagct caaagacttc
    91921 aatatcaaga cagatttagg tccagcttct caggaagtgg atctgattat ttcagtggaa
    91981 ggcgtacagc agaagtaaga gtgaaatgtt gccggatggc acgcatccgg caatgatatt
    92041 tattctgttg gatcaggaat acccagtgtt gtattcagac gtccacgaga cttattaaag
    92101 atcttattgc cattttcacg tcctgcccga cggcgtcgct gttcttctgg cggtaacgcg
    92161 ctttcttcgc agcaaatctc actacaacaa cctttgtatt tttccgcgca tactggacac
    92221 tgaataaaca gcaggtggca gccatcattt ttacagttgg tatggctgtc gcacggcgcg
    92281 ccgcactggt ggcaatgcgc gataatctca tccgatatac gttcgcccat ccgctcgtca
    92341 aaaacaaaat ttttgccaat aaaacgcacc ggcaagccct gctcgcgcgc cttacgggcg
    92401 tattcaataa ttccgccctc gatatgccag actttgttga atccgttatg tttcatccag
    92461 gcactggcct tttcacaacg aatgccgccg gtgcagtaca tgacgatttt tttatcttta
    92521 tgtgcctgca tcatctcgac tgcttttggc agctgctcac ggaaggtatc tgccggaatt
    92581 tcgagcgcgt tttcaaagtg ccccacttca tactcatagt ggttacgcat gtcgataaat
    92641 agtgcatcgg gatcgtcaag catggcgttc acttccgccg cttgcagata ctcaccaaca
    92701 ttgctggcat caaagtgagg atcgtcaata ccgtcggcaa caatgcgatc gcgtaccttc
    92761 atgcgcagta cccagaagga tttcccgtca tcatccaacg cgatattcag gcgtaaaccc
    92821 tctaaagccg ggtcgaaggc atagagctgc gcgcgaaatg tttcaacatt gctcgcaggt
    92881 acgctgattt gcgcgttaat gccctcatgc gccagataca ctcgcccaaa aacattcagc
    92941 gcggtaaaca gctgatataa agcgtcacgg gtcgccttag gatcggcgat gtggaaatac
    93001 ttgtaaaacg aaatggtggt tcgcggttcg ctctcagcca acattttggc ttttaacgcg
    93061 tcgttggaaa tgcggttgtg taacactggc atggtgtacg gttcctgcga gatgggaaag
    93121 taaaaatccg cggcatgata tagcaattat cgataattaa catccacaca ttttacgcta
    93181 catttgcgca ttaaaaatta tttgttattt acaagcgcgg caatttcgcc cagtcttcag
    93241 ccacaaattt tggttgcggg cgaaaaaatg cgacaataca tacaattgcc cgtataggtt
    93301 gaaaaacagg attgatatga cgaatctacc caagttctcc accgcactgc ttcatccgcg
    93361 ttattggtta acctggttgg gtattggcgt actttggttg gtcgtgcaat tgccctaccc
    93421 gattatctac cgcctcggtt gtggattagg aaaactggcg ttacgtttta tgaaacgacg
    93481 cgcaaaaatt gtgcatcgca acctggaact gtgcttcccg gaaatgagcg aacaagaacg
    93541 ccgtaaaatg gtggtgaaga atttcgaatc cgttggcatg ggcctgatgg aaaccggtat
    93601 ggcgtggttc tggccggacc gccgaatcgc ccgctggacg gaagtgatcg gcatggaaca
    93661 cattcgtgac gtgcaggcgc aaaaacgcgg catcctgtta gttggcatcc attttctgac
    93721 actggagctg ggcgcgcggc agtttggtat gcaggaaccg gggattggcg tttaccgccc
    93781 gaacgataat ccactgattg actggctaca aacctggggc cgtttgcgct caaataaatc
    93841 gatgctcgac cgcaaagatt taaaaggcat gattaaagcc ctgaaaaaag gcgaagtggt
    93901 ctggtacgca ccagatcatg attacggccc gcgctcaagc gttttcgtcc cgttatttgc
    93961 cgttgagcag gctgcgacca cgaccggaac ctggatgctg gcacggatgt ccggcgcatg
    94021 tctggtgccc ttcgttccac gccgtaagcc agatggcaaa gggtatcaat tgattatgct
    94081 gccaccagag tgttctccgc cactggatga tgccgaaact accgccgcgt ggatgaacaa
    94141 agtggtcgaa aaatgcatca tgatggcacc agagcagtat atgtggttac accgtcgctt
    94201 taaaacacgc ccggaaggcg ttccttcacg ctattaaatc tcccatgccg gatgcttcag
    94261 aatggcatcc ggcattacca cagcaaatcc ccctgattta gcgataaaag ctctctggat
    94321 tgcgccccct ggaagtcggg cgcataatta gtgtgtttat cttttcttct tatgttcacc
    94381 gcgcctggcg caccaacagc ggattgctat gtcaccctgt gaaaatgaca cccctataaa
    94441 ctggaaacga aacctgatcg tcgcctggct aggctgtttt cttaccgggg ccgccttcag
    94501 tctggtaatg cccttcttac ccctctacgt tgagcagctt ggtgttaccg gccactccgc
    94561 cctgaatatg tggtccggta ttgtcttcag tataatgggt tcctaaacac atcattttag
    94621 gtattgaata tgagacgggc aatcagctac atcagatttt caagtgaacg acaactgaaa
    94681 ggggactctg tacgccgaca atctaagctg gttactgact ggcttgataa gaaccctgag
    94741 ttttacttgg attcatcctt gagctttaaa gatttgggga aaagtgcttt tagtggtaag
    94801 cacctgaaag gtggtctagg tgacttttta accgctatcg agaaagggct tgttaaagct
    94861 ggtgatacgc tactaatcga gagtttagac cgtctgtcac gtcaggatat tgatattgct
    94921 tctgagctat tacgccgtat cttaagggct ggtgtagatg tagtcacact gagcgatgga
    94981 gaacattaca cacgagaaag tttaaaagac ccgttagccc tgattaaaag cattctgata
    95041 atgcagcggg cgcatgagga aagtttgaga aagtctgagc gtgtccaagc tgcatggaac
    95101 cgtaaaaaag aactcatctc cgaaggtatc aaggtatcta ggcgttgtcc tgcatggtta
    95161 aggctgaatg atgaccgtcg aacattcaca atcattcctg ataaagtgga agttgttaaa
    95221 cgagcatttg acctgagatt acagggttta agcttttggg ctatcaccag aactttaaac
    95281 gatgaaggtc acttgtcatt gaatcaatac acacccaaac aaaaggggtg gtctgatact
    95341 gctgtaaaga aactgctcag aaatcgtgct gtgattggat gtttcacacc tgctggacgt
    95401 gaagaagttc agggttacta ccccgcaata atcagtgagt cactattcta tcgagtacag
    95461 caactgaaca cagggcaata tggtagggct tctgtatcaa gtaatccgct atcagtcaac
    95521 ttgttcaggg gtatcatcaa
//
LOCUS       DAJTSA010000003        75634 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000003 DAJTSA010000000
VERSION     DAJTSA010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.003"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(435..641)
                     /locus_tag="OO369_000205"
     CDS             complement(435..641)
                     /locus_tag="OO369_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR5980621.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="OO369_000206"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="OO369_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR5980622.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2716
                     /gene="kdpA"
                     /locus_tag="OO369_000207"
     CDS             1043..2716
                     /gene="kdpA"
                     /locus_tag="OO369_000207"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR5980623.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2739..4786
                     /gene="kdpB"
                     /locus_tag="OO369_000208"
                     /pseudo
     CDS             2739..4786
                     /gene="kdpB"
                     /locus_tag="OO369_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4795..5367
                     /gene="kdpC"
                     /locus_tag="OO369_000209"
     CDS             4795..5367
                     /gene="kdpC"
                     /locus_tag="OO369_000209"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR5980624.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5360..8040
                     /gene="kdpD"
                     /locus_tag="OO369_000210"
                     /pseudo
     CDS             5360..8040
                     /gene="kdpD"
                     /locus_tag="OO369_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8037..8722
                     /gene="kdpE"
                     /locus_tag="OO369_000211"
                     /pseudo
     CDS             8037..8722
                     /gene="kdpE"
                     /locus_tag="OO369_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            8997..9101
                     /locus_tag="OO369_000212"
     CDS             8997..9101
                     /locus_tag="OO369_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR5980625.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9412..11609
                     /gene="speF"
                     /locus_tag="OO369_000213"
                     /pseudo
     CDS             9412..11609
                     /gene="speF"
                     /locus_tag="OO369_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11606..12925
                     /gene="potE"
                     /locus_tag="OO369_000214"
     CDS             11606..12925
                     /gene="potE"
                     /locus_tag="OO369_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR5980626.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12981..14621)
                     /gene="pgm"
                     /locus_tag="OO369_000215"
     CDS             complement(12981..14621)
                     /gene="pgm"
                     /locus_tag="OO369_000215"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR5980627.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14647..15192)
                     /gene="seqA"
                     /locus_tag="OO369_000216"
     CDS             complement(14647..15192)
                     /gene="seqA"
                     /locus_tag="OO369_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR5980628.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15377..16141
                     /gene="ybfF"
                     /locus_tag="OO369_000217"
     CDS             15377..16141
                     /gene="ybfF"
                     /locus_tag="OO369_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5980629.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16281..16574
                     /gene="ybfE"
                     /locus_tag="OO369_000218"
     CDS             16281..16574
                     /gene="ybfE"
                     /locus_tag="OO369_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR5980630.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16714..17244
                     /gene="fldA"
                     /locus_tag="OO369_000219"
     CDS             16714..17244
                     /gene="fldA"
                     /locus_tag="OO369_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR5980631.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17454..17540
                     /gene="uof"
                     /locus_tag="OO369_000220"
     CDS             17454..17540
                     /gene="uof"
                     /locus_tag="OO369_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR5980632.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17533..17979
                     /gene="fur"
                     /locus_tag="OO369_000221"
     CDS             17533..17979
                     /gene="fur"
                     /locus_tag="OO369_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR5980633.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18063..18389)
                     /locus_tag="OO369_000222"
     CDS             complement(18063..18389)
                     /locus_tag="OO369_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5980634.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18426..19832)
                     /gene="chiP"
                     /locus_tag="OO369_000223"
     CDS             complement(18426..19832)
                     /gene="chiP"
                     /locus_tag="OO369_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR5980635.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20411..22075)
                     /gene="glnS"
                     /locus_tag="OO369_000224"
     CDS             complement(20411..22075)
                     /gene="glnS"
                     /locus_tag="OO369_000224"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR5980636.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22278..24224)
                     /gene="nagE"
                     /locus_tag="OO369_000225"
     CDS             complement(22278..24224)
                     /gene="nagE"
                     /locus_tag="OO369_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR5980637.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWLPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24557..25357
                     /gene="nagB"
                     /locus_tag="OO369_000226"
     CDS             24557..25357
                     /gene="nagB"
                     /locus_tag="OO369_000226"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5980638.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25417..26565
                     /gene="nagA"
                     /locus_tag="OO369_000227"
     CDS             25417..26565
                     /gene="nagA"
                     /locus_tag="OO369_000227"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5980639.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26574..27794
                     /gene="nagC"
                     /locus_tag="OO369_000228"
                     /gene_synonym="nagR"
     CDS             26574..27794
                     /gene="nagC"
                     /locus_tag="OO369_000228"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR5980640.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27842..28594
                     /gene="nagD"
                     /locus_tag="OO369_000229"
                     /pseudo
     CDS             27842..28594
                     /gene="nagD"
                     /locus_tag="OO369_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            28991..30655
                     /gene="asnB"
                     /locus_tag="OO369_000230"
     CDS             28991..30655
                     /gene="asnB"
                     /locus_tag="OO369_000230"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR5980641.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            31034..31110
                     /locus_tag="OO369_000231"
     tRNA            31034..31110
                     /locus_tag="OO369_000231"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31068..31070,aa:Met,seq:cat)
     gene            31119..31203
                     /locus_tag="OO369_000232"
     tRNA            31119..31203
                     /locus_tag="OO369_000232"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31153..31155,aa:Leu,seq:tag)
     gene            31227..31301
                     /locus_tag="OO369_000233"
     tRNA            31227..31301
                     /locus_tag="OO369_000233"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31259..31261,aa:Gln,seq:ttg)
     gene            31336..31410
                     /locus_tag="OO369_000234"
     tRNA            31336..31410
                     /locus_tag="OO369_000234"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31368..31370,aa:Gln,seq:ttg)
     gene            31426..31502
                     /locus_tag="OO369_000235"
     tRNA            31426..31502
                     /locus_tag="OO369_000235"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31460..31462,aa:Met,seq:cat)
     gene            31551..31625
                     /locus_tag="OO369_000236"
     tRNA            31551..31625
                     /locus_tag="OO369_000236"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31583..31585,aa:Gln,seq:ctg)
     gene            31663..31737
                     /locus_tag="OO369_000237"
     tRNA            31663..31737
                     /locus_tag="OO369_000237"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31695..31697,aa:Gln,seq:ctg)
     gene            complement(31891..33066)
                     /gene="ubiF"
                     /locus_tag="OO369_000238"
     CDS             complement(31891..33066)
                     /gene="ubiF"
                     /locus_tag="OO369_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR5980642.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            33212..34636
                     /gene="miaB"
                     /locus_tag="OO369_000239"
     CDS             33212..34636
                     /gene="miaB"
                     /locus_tag="OO369_000239"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR5980643.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            34789..35829
                     /locus_tag="OO369_000240"
     CDS             34789..35829
                     /locus_tag="OO369_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR5980644.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            35826..36293
                     /gene="ybeY"
                     /locus_tag="OO369_000241"
     CDS             35826..36293
                     /gene="ybeY"
                     /locus_tag="OO369_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR5980645.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            36383..37261
                     /gene="corC"
                     /locus_tag="OO369_000242"
     CDS             36383..37261
                     /gene="corC"
                     /locus_tag="OO369_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR5980646.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            37286..38824
                     /gene="lnt"
                     /locus_tag="OO369_000243"
     CDS             37286..38824
                     /gene="lnt"
                     /locus_tag="OO369_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR5980647.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            39221..40129
                     /gene="gltI"
                     /locus_tag="OO369_000244"
     CDS             39221..40129
                     /gene="gltI"
                     /locus_tag="OO369_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR5980648.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            40299..41039
                     /gene="gltJ"
                     /locus_tag="OO369_000245"
     CDS             40299..41039
                     /gene="gltJ"
                     /locus_tag="OO369_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR5980649.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMALVERKVRLPGNMGGK"
     gene            41039..41713
                     /gene="gltK"
                     /locus_tag="OO369_000246"
     CDS             41039..41713
                     /gene="gltK"
                     /locus_tag="OO369_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR5980650.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            41713..42438
                     /gene="gltL"
                     /locus_tag="OO369_000247"
     CDS             41713..42438
                     /gene="gltL"
                     /locus_tag="OO369_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR5980651.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            42556..43491
                     /gene="rihA"
                     /locus_tag="OO369_000248"
     CDS             42556..43491
                     /gene="rihA"
                     /locus_tag="OO369_000248"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR5980652.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            43575..45245
                     /gene="hscC"
                     /locus_tag="OO369_000249"
                     /pseudo
     CDS             43575..45245
                     /gene="hscC"
                     /locus_tag="OO369_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(45305..46756)
                     /gene="djlC"
                     /locus_tag="OO369_000250"
     CDS             complement(45305..46756)
                     /gene="djlC"
                     /locus_tag="OO369_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR5980653.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(46753..47460)
                     /locus_tag="OO369_000251"
     CDS             complement(46753..47460)
                     /locus_tag="OO369_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5980654.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            47562..48116
                     /locus_tag="OO369_000252"
     CDS             47562..48116
                     /locus_tag="OO369_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5980655.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(48126..49553)
                     /locus_tag="OO369_000253"
     CDS             complement(48126..49553)
                     /locus_tag="OO369_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR5980656.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(49550..50257)
                     /locus_tag="OO369_000254"
     CDS             complement(49550..50257)
                     /locus_tag="OO369_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5980657.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            50421..51398
                     /locus_tag="OO369_000255"
     CDS             50421..51398
                     /locus_tag="OO369_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5980658.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(51468..51950)
                     /locus_tag="OO369_000256"
     CDS             complement(51468..51950)
                     /locus_tag="OO369_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR5980659.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            52185..54767
                     /gene="leuS"
                     /locus_tag="OO369_000257"
     CDS             52185..54767
                     /gene="leuS"
                     /locus_tag="OO369_000257"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR5980660.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            54782..55363
                     /gene="lptE"
                     /locus_tag="OO369_000258"
     CDS             54782..55363
                     /gene="lptE"
                     /locus_tag="OO369_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR5980661.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            55363..56394
                     /gene="holA"
                     /locus_tag="OO369_000259"
     CDS             55363..56394
                     /gene="holA"
                     /locus_tag="OO369_000259"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR5980662.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            56396..57037
                     /gene="nadD"
                     /locus_tag="OO369_000260"
     CDS             56396..57037
                     /gene="nadD"
                     /locus_tag="OO369_000260"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR5980663.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            57061..57672
                     /locus_tag="OO369_000261"
     CDS             57061..57672
                     /locus_tag="OO369_000261"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR5980664.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            57932..58249
                     /gene="rsfS"
                     /locus_tag="OO369_000262"
                     /gene_synonym="rsfA"
     CDS             57932..58249
                     /gene="rsfS"
                     /locus_tag="OO369_000262"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR5980665.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            58253..58720
                     /gene="rlmH"
                     /locus_tag="OO369_000263"
     CDS             58253..58720
                     /gene="rlmH"
                     /locus_tag="OO369_000263"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR5980666.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            58751..60652
                     /gene="mrdA"
                     /locus_tag="OO369_000264"
     CDS             58751..60652
                     /gene="mrdA"
                     /locus_tag="OO369_000264"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR5980667.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            60655..61767
                     /gene="mrdB"
                     /locus_tag="OO369_000265"
                     /gene_synonym="rodA"
     CDS             60655..61767
                     /gene="mrdB"
                     /locus_tag="OO369_000265"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR5980668.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            61778..62866
                     /gene="rlpA"
                     /locus_tag="OO369_000266"
     CDS             61778..62866
                     /gene="rlpA"
                     /locus_tag="OO369_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR5980669.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            63005..64216
                     /gene="dacA"
                     /locus_tag="OO369_000267"
     CDS             63005..64216
                     /gene="dacA"
                     /locus_tag="OO369_000267"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR5980670.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            64327..64590
                     /locus_tag="OO369_000268"
     CDS             64327..64590
                     /locus_tag="OO369_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR5980671.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            64691..65332
                     /gene="lipB"
                     /locus_tag="OO369_000269"
     CDS             64691..65332
                     /gene="lipB"
                     /locus_tag="OO369_000269"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR5980672.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            65591..66544
                     /locus_tag="OO369_000270"
     CDS             65591..66544
                     /locus_tag="OO369_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5980673.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            66753..67718
                     /gene="lipA"
                     /locus_tag="OO369_000271"
     CDS             66753..67718
                     /gene="lipA"
                     /locus_tag="OO369_000271"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR5980674.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(67819..68022)
                     /gene="tatE"
                     /locus_tag="OO369_000272"
     CDS             complement(67819..68022)
                     /gene="tatE"
                     /locus_tag="OO369_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR5980675.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(68151..68939)
                     /locus_tag="OO369_000273"
     CDS             complement(68151..68939)
                     /locus_tag="OO369_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR5980676.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            69032..69415
                     /gene="crcB"
                     /locus_tag="OO369_000274"
     CDS             69032..69415
                     /gene="crcB"
                     /locus_tag="OO369_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR5980677.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSTEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(69469..69678)
                     /gene="cspE"
                     /locus_tag="OO369_000275"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(69469..69678)
                     /gene="cspE"
                     /locus_tag="OO369_000275"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5980678.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(69853..70413)
                     /gene="pagP"
                     /locus_tag="OO369_000276"
     CDS             complement(69853..70413)
                     /gene="pagP"
                     /locus_tag="OO369_000276"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR5980679.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            71002..72387
                     /gene="dcuC"
                     /locus_tag="OO369_000277"
     CDS             71002..72387
                     /gene="dcuC"
                     /locus_tag="OO369_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5980680.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(72428..72769)
                     /locus_tag="OO369_000278"
                     /pseudo
     CDS             complement(72428..72769)
                     /locus_tag="OO369_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(73107..73616)
                     /locus_tag="OO369_000279"
     CDS             complement(73107..73616)
                     /locus_tag="OO369_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5980681.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            73779..74024
                     /locus_tag="OO369_000280"
     CDS             73779..74024
                     /locus_tag="OO369_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5980682.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            73999..75630
                     /locus_tag="OO369_000281"
                     /pseudo
     CDS             73999..75630
                     /locus_tag="OO369_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
BASE COUNT        18617 a        18996 c        19209 g        18812 t
ORIGIN      
        1 cataacatct tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag
       61 tatatattac tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc
      121 ggaatttatt tagatgctgg aattagaaaa agggatgacg gctttctcac cttaccctga
      181 agtttcacga aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt
      241 cgcttagagc gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg
      301 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
      361 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      421 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctacccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttactac tcatccgacc acttattttt gcttattgat ggtttattta
      781 cattcacact gtaattaaat cacacaaaag ttaaattaat actaaccatc agttaaatca
      841 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctactctt ccggtatcac ttttaggcca ctggaggtgc actgtgagtg
      961 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggctatttg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
     1081 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
     1141 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
     1201 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
     1261 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1321 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1381 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
     1441 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
     1501 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1561 cacgttatgg gtgctagtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg
     1621 tgcgcagcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
     1681 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1741 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1801 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
     1861 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1921 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1981 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     2041 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
     2101 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
     2161 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     2221 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatag acgtacgcga
     2281 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     2341 gttggcgatg atgaccgacg ccggacgtag cgccatgctt aaccctagcc cgcatggttt
     2401 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     2461 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2521 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2581 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2641 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2701 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2761 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2821 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2881 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2941 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     3001 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca
     3061 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     3121 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     3181 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     3241 aatccggcgg cgattttgcc tccgtcaccg gcggcacgcg tattctttct gactggctgg
     3301 tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc gcgatggtgg
     3361 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3421 tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3481 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3541 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3601 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3661 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     3721 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3781 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3841 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3901 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     3961 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     4021 ccacgctgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     4081 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt
     4141 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     4201 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     4261 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctacgg cgctggcgca
     4321 ggcagatgtc gcggtggcga tgaactccgg cacccaggcg gctaaagagg cgggcaatat
     4381 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     4441 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4501 cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     4561 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4621 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4681 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4741 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     4801 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     4861 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     4921 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     4981 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     5041 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     5101 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     5161 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     5221 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     5281 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     5341 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     5401 ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     5461 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5521 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5581 gctggctgtt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5641 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5701 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5761 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5821 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5881 cgacgacgtg gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg
     5941 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     6001 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     6061 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     6121 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctgacg
     6181 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta
     6241 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6301 gagacggcaa cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa
     6361 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     6421 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6481 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     6541 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     6601 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     6661 ggcgtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgccac cgtcattaat
     6721 gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgccgt ctctgatgtg
     6781 caatatctgc tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg gaaccttact
     6841 gctggtgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac acggcactta
     6901 tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc cgccaccagc
     6961 gaacaattta ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac
     7021 ggtaaattgc agccgttaac acatccgcaa ggaattacgc catgggacga tgccatcgcg
     7081 cagtggagtt ttgataaagg cctgcctgcg ggcgcgggca ccgacacgtt acccggtgta
     7141 ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acgggctggt ggtggtggag
     7201 ccggggaatc tgcgccagtt gatgatcccg gaacagcagc gcctgctgga gacttttacg
     7261 ctgttagtcg ccaatgccct tgagcggctg acgctaaccg ccagcgaaga acaggcgcgg
     7321 atggcaagcg aacgtgaaca gatccgcaac gccctgctgg cagcgctttc gcatgattta
     7381 cgcacgccgc ttacggtgct gtttggtcag gcagaaatct taacgctcga tctggcaagc
     7441 gaaggatcac cccacgcccg ccaggccagc gagattcgtc agcatgtgct gaacactacc
     7501 cgactggtga ataatctact ggatatggcg cgaattcagt ccggcggctt taatttgaag
     7561 aaagagtggt taacgctgga agaagtagtc ggcagcgcgc tgcaaatgcc ggaaccgggt
     7621 ttatcgtcgc ccatcaatct ttctctgcca gaaccgctga ccttaatcca cgttgacggg
     7681 ccactctttg aacgggtgct gattaatctg ctggagaacg cggtgaaata tgcgggtgcg
     7741 caggccgaaa ttggtatcga tgcccacgtt gagggcgaaa atctacaact ggatgtctgg
     7801 gataacggcc ccggtcttcc gccaggccag gagcagacga tatttgataa gtttgctcgc
     7861 gggaataaag agtcggcggt gccgggtgtc gggcttgggt tggcaatttg tcgggcgatt
     7921 gtggatgtac acggcggcac cattaccgcg ttcaaccgac cggaaggtgg tgcctgtttt
     7981 cgtgttacac ttccccagca aaccgctcct gaacttgaag actttcatga ggatatgtga
     8041 caaacgttct gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc acggcgctgg
     8101 agggcgacgg gatgcgcgtc tttgaggccg aaactctgca acgcggcttg ctggaagcgg
     8161 caacccgtaa gccagatttg attattctcg atctcggcct gcccgatggt gatgggattg
     8221 agtttatccg cgacctgcgc cagtggagcc cggtgccggt gattgtgctt tccgcacgca
     8281 gcgaagagag cgacaagatc gccgcgctgg atgccggagc ggatgattat ctgagtaagc
     8341 cgtttggtat tggcgaattg caggcccgtc tgcgcgtcgc tttacgccgc cactccgcca
     8401 ctccgccacc gccgcgcccg atccgctggt gaaattttcc ggtgttaccg tcgatttagc
     8461 cgcccgcgtg attcaccggg gtgaggaaga ggtgcatctc acaccaattg agttccgcct
     8521 gctggcggtg ctgctcaaca atgccggaaa agtactcacc cagcgccagc tccttaacca
     8581 ggtgtggggg ccaaacgcgg tcgaacacag tcactatttg cgtatttata tgggacatct
     8641 gcgacaaaaa ctggaacagg atccggcccg gcctcgccac ttcattactg aaaccggtat
     8701 tgggtatcgg tttatgcttt gaattgttat tcaataaatt cgcttaaata gtgattaatt
     8761 tattattaaa cctgcgccga atattattat ttggcaggtt taatttctta acgcaaatat
     8821 aaaacacgaa attacaacat ttaagtaaca aaacagcatc taatatggcg atattcataa
     8881 ttaatgattt attatctggg gtatgatcgt tttttgttga tcttcttcac agattatagc
     8941 catttcatgg atagaataac tctaccttca actgacacag caagcggtaa aggtaaatgg
     9001 aaaataataa ccgcttaatg cctcatataa ggcggacaac ccatatcatg aagtttgccc
     9061 atcgtaatag ttttgacttt catttcttta atgcccgcta gtcttctgac taaagggcac
     9121 cccaacgtac aggtctccct gactttaagc attacaggtt aatacctgta ttcctcggtg
     9181 ctcatatact gctaaccctt ttaaactcta aataattcga gtcgcagcac ttgcaacttg
     9241 aggtatgacg agtatagcca gttaccgggc tggtctgggt tattgcatct gcaaaaagca
     9301 aactactgat ttatttatca gcggtggagc tttgcttttt ttccgacgtg atcgatttct
     9361 cctttgagaa attgaggacc tgctattacc tgaaataaag agatgaataa aatgtcagaa
     9421 ttaaaaattg ccgttagccg ttcttgcccg gattgttttt ccactcatcg tgcatgcgtg
     9481 aatattgacg aaagtaatta tattgacgtt gccgccatta ttttatcagt caatgatgtt
     9541 gaacgtggaa aactcgataa aatagacgct actggctatg gcattcctgt ttttattgca
     9601 acggaaaatg aagaacgtgt accagcagaa tatctgccac gtatttctgg tgtcttcgag
     9661 cattgcgaat cacggaaaga gttttatgga cgtcagttag aaaccgctgc tagccattat
     9721 gaaacgcaac tgcgcccgcc cttcttccgc gcactggtcg attatgtcaa tcagggcaat
     9781 agcgcttttg actgcccggg tcatcaggga ggtgagtttt tccgtcgtca tccggcaggg
     9841 aatcagttcg tggagtactt cggagaaacg ctgttccgtt ccgacttatg caacgcagac
     9901 gtagcaatgg gcgatctgct gattcacgaa ggcgcgccat gcattgcaca gcaacatgcg
     9961 gcaaaagtgt ttaatgccga taaaacatac ttcgttctaa atggcacttc atcttctaac
    10021 aaagtggttt taaacgccct gctaacaccg ggtgatctgg tgctgtttga tcgcaataac
    10081 cacaaatcta accaccacgg agcgttgcta caggctggtg caacaccggt ttatctggaa
    10141 acggcacgta acccgtatgg ctttatcggt ggcattgatg cgcactgttt tgaagaaagt
    10201 tacctgcgtg agctgatcgc ggaagtcgca ccgcagcggg caaaagaggc tcgtcctttc
    10261 cgcctcgctg tgattcagtt aggcacctac gacggtacga tttataacgc ccgccaggtg
    10321 gtggataaaa ttggtcatct gtgtgactac attctgtttg actccgcatg ggttggctat
    10381 gagcaattta tcccgatgat ggctgactgt tcgccgctgt tgctggatct taatgagaac
    10441 gatccgggta ttctagttac gcaatccgta cataaacagc aggctggttt ttctcaaact
    10501 tcacaaatcc ataaaaaaga cagccacatc aaagggcaac agcgttatgt accgcacaaa
    10561 cgcatgaaca acgcctttat gatgcacgcc tccaccagcc cgttctatcc gctgtttgcc
    10621 gcactggata tcaacgccaa aatgcatgaa ggtgtcagcg gtcgtaatat gtggatggat
    10681 tgtgtggtaa atggcattaa tgcccgcaaa ctgatcctcg ataactgtca gcatattcgt
    10741 ccgttcgtac ctgaactggt ggatggtaaa ccctggcagt cgtatgaaac agcgcaaatt
    10801 gcggttgatc tgcgcttctt ccagtttgta ccaggggaac actggcattc ttttgaaggc
    10861 tatgcagaga atcaatactt tgtcgatcct tgcaaactgt tgctgacaac cccaggtatt
    10921 gatgcacgta acggcgaata tgaagcgttc ggtgtacccg cgacgattct tgctaacttc
    10981 ctgcgcgaaa atggcgtagt gccggaaaaa tgcgatctta acgccatcct cttcctgctg
    11041 actccggcag aagatatggc caaacttcag caacttgtgc cctgctggta cgcttcgaaa
    11101 aactgcttga gtccgacgcg ccattagcag aagtgctgcc ttccatctac aaacagcatg
    11161 aagagcgcta cgccggttat accctgcgtc agttgtgcca ggaaatgcat gatttgtatg
    11221 cccgccacaa cgtgaaacaa ctgcaaaaag agatgttccg caaagcgcac ttcccacacg
    11281 tcagcatgaa tccgcaagaa gccaactacg cctatttacg cggtgaagtg gagctggttc
    11341 gtctaccgga tgccgaaggt cgtatcgctg ccgaaggggc gcttccttat cctccgggtg
    11401 tgctgtgtgt tgttccgggt gaaatctggg gtggtgctgt tttgcgttac ttcagcgctc
    11461 tggaagaagg gatcaacctg ctgccaggtt ttgcaccgga gctgcagggt gtctatatcg
    11521 aagaacatga tggtcgtaag caagtttggt gctatgtcat caagcctcgt gatgcgcaaa
    11581 gcaccctgtt gaaaggggaa aaattatgag tcaggctaaa tcgaacaaaa tgggcgtcgt
    11641 tcagttaacc atactgacga tggtcaacat gatgggctcc ggtatcatca tgctgccgac
    11701 aaagcttgcc gaagttggga caatttcaat tatctcctgg ctggtgacag ccgtaggctc
    11761 aatggcactg gcatgggcat tcgcaaaatg cggtatgttc agccgtaaat caggtggtat
    11821 gggcggttat gccgagtatg ctttcggtaa atccggtaac tttattgcga actataccta
    11881 cggcgtctca ctgctgattg ctaacgtcgc gattgctatt tcggcggttg gttacggcac
    11941 cgaactgctc ggcgcgagtt tgtcgccagt gcagattggt cttgcgacca tcggggtgct
    12001 gtggatttgt accgtggcta actttggtgg tgcgcgcatt accgggcaaa tcagtagcat
    12061 taccgtgtgg gggggcatta ttccggtggt aggcctgtgc attatcggct ggttctggtt
    12121 tagcccgacg ctttacgttg attcctggaa tccgcatcat gcaccgttct tcagtgcagt
    12181 aggttcttcc atcgctatga cgctgtgggc ttttcttggt ctggagtctg cgtgtgcgaa
    12241 tactgatgta gtggaaaacc cggaacgtaa tgtgccaatc gcggtactcg gcggtacgtt
    12301 aggtgcggcg gtgatttata tcgtctccac caacgtgatt gcagggattg tgcctaatat
    12361 ggagctggca aattcaacgg caccatttgg cctggccttc gcacagatgt ttacaccaga
    12421 agtgggtaaa gtcattatgg cgctgatggt gatgtcctgc tgcggttcgc tgcttggctg
    12481 gcagttcacc attgcccagg tgtttaaatc ttcatctgat gaaggctact tccctaaaat
    12541 tttctcccgt gtaaccaaag tggatgcacc ggtgcaggga atgttgacca ttgtgattat
    12601 ccagagcggg ttggcgctga tgacgattag cccgtcgcta aacagtcagt tcaacgtgct
    12661 ggttaacctg gccgtggtga ccaatatcat tccgtatatt ctgtcaatgg cggcattagt
    12721 cattattcag aaggtcgcta atgtgccccc ctcaaaagcg aaagttgcaa actttgttgc
    12781 ttttgttggc gcgatgtata gcttttatgc gctgtactca tccggggaag aagccatgct
    12841 gtacggttcc atcgtgacct tccttggttg gacactgtat ggtctggtat caccacgctt
    12901 tgaactgaaa aataaacacg gttaaaaaag caaagtaaaa aagggcgatc ttgcgaccgc
    12961 tcttttttta ttaaatgtgt ttacgcgttt ttcagaactt cgctaacaat ctcaaccgct
    13021 tctttctcaa tctgcttgcg atgttcttca ccgaggaagc tttcgcagta gatcttatat
    13081 gcgtcttccg tgcctgacgg acgcgcggcg aaccagccgt tgtcagtcat cactttcaga
    13141 ccgccaatag aagcaccgtt gcccggagca gcagtcaggc gcgcggtgat cgggtcacct
    13201 gccagggtgc tggcgctcac catttccgga gacagcttag acagcgccgc tttttgtgcg
    13261 gaagtcgcag ctgcctgcaa acggttgtag ctcggcgcac caaagcgttt tgccagttcg
    13321 ttgtagtgtt cctgcgggtt cttaccggtg acagcggtga tttccgccgc cagcagacac
    13381 ataatgatgc cgtctttgtc ggtggaccac ggcgtgccgt cgaaacgcag gaaggaagcc
    13441 cctgcgctct cttcgccgcc aaagccgaag ctgccgtcga acagaccatc aacaaaccat
    13501 ttgaaaccta ccggtacttc taccagctta cggcccaagt cattgaccac acggtcgatc
    13561 atcgcagaag aaaccagcgt tttaccgaca gcaacatctt tgccccactg cggacgatgc
    13621 tggaacaggt agttgatcgc caccgccagg tagtggttcg gattcatcaa acctgccgga
    13681 gtgacgatac cgtgacggtc ataatccggg tcgttagcaa acgccagatc gaacttatca
    13741 cgcagtgcca gcagacccgc catcgcacac tcggaggagc agtccatacg gatcgcaccg
    13801 tctttatcga ggtgcataaa gcggaaggtt tgatcgacct gatcgttaac gatggtcagg
    13861 ttgaggttgt aatactcgcc aatgcgcttc cagtattcga taccggaacc gcccaacgga
    13921 tcaacgccca gcgtcaggcc cgctttctga atcgccgcca tatcaacgat atcggccagc
    13981 ccttccacga acggctgcac cagatcctgc tctttcacat agccggatgc catcgcttcg
    14041 tcgagggaga tacgcttcac gcctttcagg ccatcggcca tcagtgcgtt ggccttgtct
    14101 tccaccactt tggtgacgtt agtatcagcc gggccaccat ttggcggatt gtacttgata
    14161 ccaccatctt ccggcgggtt atgggacggt gtaatcacga taccgtctgc cagcgggcca
    14221 ccttttttat tgtgaaccag gatggcattg gaaacggcag gcgtcggagt gaagccattg
    14281 ttttcctgca caatgacatc aacgccgttc gctgccagca cttccagtac ggaaatgaac
    14341 gcaggctcgg acagggcgtg agtatcttta cccacatagc aagggccagt gatgccgttt
    14401 ttcgcacgtt cttcagcaat tgcctgagcg atcgccagaa tgtgcggctc gttaaagctg
    14461 tggcgcgctg cactgccacg gtgaccggaa gtaccgaatt tcaccgcgtg ctccgcattc
    14521 cctgcttctg gtttcagtac ataatattgc gccgtcagtt gggcgacgtt aatcaaatca
    14581 ctctgttgtg caggttggcc tgcacgattg tggattgcca ttgctttgtc ctttgtctgc
    14641 aacgttttag atagttccgc aaaccttctc aatcaattcc gccgggaatt gcatcgactg
    14701 catgatgtgt tcgatcatgc tgcatttacg gccggtgttg gtgttggtga tcacccaata
    14761 cggcgtgcct ggcacatgtt tcggcttggt ctgattacca tttttcagca gcgtttgttc
    14821 atctgccgca aagtacacgc gtgtacgacc gtgcaacgat tccgttgctt cggcaaacgc
    14881 ctgggcgtca agagaatata gtgtagacaa cagcagcata aagcgattga ccgctcgctt
    14941 ttgctctgcg tattcgtccg aaagcagaag ttcacgcatt gcgcgaacct tgtctttaat
    15001 cgttttgacc ggcttcgctt cgacgatagc aggtgacgca acgcgaacct ctttcgtcac
    15061 cggagcagca ggctgtgatg cggcggaaaa tttcaacata cgccgtaaaa tgtcggatgc
    15121 gctctcgccg atatgcttag tgtggctggc aatatagctg tagagttcat catcaacttc
    15181 aatcgttttc atcttaatcc agtgcagtgt cttagctgaa tacaagtcgc caagagtata
    15241 tgaataaatc ccggcagcgg atagcgtcaa gcctggcttg cagaaaaact cggaataaac
    15301 cttatttgtt gcagccctgt ggcacaatgg tcaacataat accctaaccc gacaacgcga
    15361 aaaaaagaac tttgccatga aattaaatat ccgcgcgcaa actgcacaaa accagcacaa
    15421 taattctccc atcgttcttg tccatggtct gtttggtagc ctcgacaacc ttggcgtact
    15481 ggctcgcgat ctggtaaacg atcacaatat catccaggtt gatatgcgta accacggtct
    15541 ttcaccgaga gagccggtaa tgaattaccc ggcgatggcg caggatcttg ttgataccct
    15601 ggatgcactg cagatcgaca aagcaacatt tatcggtcac tccatgggcg gtaaagcggt
    15661 aatggcactt actgcactgg cccccgatcg catcgataaa ctggtggcga tcgatatcgc
    15721 accggtcgac tatcacgtac gccgtcatga tgagattttc gcagctatca acgcggtcag
    15781 tgaatcggac gcacaaactc gccagcaagc agcagcaata atgcgccagc atcttaatga
    15841 agaaggggtg attcagtttc tgttgaaatc ttttgttgac ggggagtggc gctttaacgt
    15901 gccggtattg tgggatcagt atccgcatat tgtgggttgg gagaaaatcc cggcatggga
    15961 tcaccctgcc ctgtttatcc ctggcggcaa ttctccgtat gttagcgagc agtaccgtga
    16021 tgatttactg gcacaatttc cacaggcacg ggcgcatgtg attgcaggcg cgggtcactg
    16081 ggtccatgct gaaaaaccgg atgcggtatt acgcgctatc cgtcgctatc tcaatgatta
    16141 acgcaactga ttaaaaaccc agcgtccgcg tcgctttccg cgcgtgggcg ttggcgtgac
    16201 tcatcgcgct gatgtattat ggcgcgctat ccatccgggc agaggcctgg ctgattgttt
    16261 cccccgaagt caccaagatc atggccaaag aacaaacgga ccgtacgaca ttagatctgt
    16321 tcgcgcacga gcgtcgaccg ggacgaccga aaactaatcc gctttcgcgc gatgaacagc
    16381 tgcgtattaa taaacgcaac cagctaaaac gcgacaaagt acgtggcctt aagcgtgtcg
    16441 aactgaagct gaacgcggaa gctgtcgagg cgctgaacga gctggcggaa tcgcgtaata
    16501 tgagccgtag cgaactgatc gaagagatgt taatgcagca actggcggcg ctgcgtagtc
    16561 agggcatcgt ttaaatttcc actttcatgt agcacagtgt gcagtcctgc tcgtttgctg
    16621 attccgcaag actgcctgtt ctgctatgat tgcctttatc cgtgggcaat tttccacccc
    16681 catttcaata agtttcaaga ggttatttca ctcatggcta tcactggcat ctttttcggc
    16741 agcgacaccg gtaataccga aaatatcgca aaaatgattc aaaaacagct tggtaaagac
    16801 gttgccgatg tccatgacat tgcaaaaagc agcaaagaag atctggaagc ttacgacatt
    16861 ctgctgctgg gcatcccaac ctggtattac ggcgaagcgc agtgtgactg ggatgacttc
    16921 ttcccgactc tcgaagagat tgatttcaat ggtaaactgg ttgcgctgtt tggttgtggt
    16981 gaccaggaag attacgccga atatttctgc gacgcattgg gcaccatccg cgacatcatt
    17041 gaaccgcgcg gtgcaaccat cgttggtcac tggccaactg cgggctatca tttcgaagca
    17101 tcaaaaggtc tggcagatga cgaccacttt gtcggtctgg ctatcgacga agaccgtcag
    17161 ccggaactga ccgctgaacg tgtagaaaaa tgggttaaac agatttctga agagttgcat
    17221 ctcgacgaaa ttcttaatgc ctgatgtgat gcggcgtaga ctcatgtcta cgccgtatta
    17281 atagataatg ccaatcaaaa taattgctac aaatttgtaa cttttgctgt tgtacctata
    17341 caatgtccca gtgttcaagt ggccttgccg ttgtaaatgt aagctgtgcc acgtttttat
    17401 taacaatatt tgccagggac ttgtggtttt catttaggcg tggcaattct ataatgatac
    17461 gcattatctc aagagcaaat tctgtcactt cttctaatga agtgaaccgc ttagtaacag
    17521 gacagattcc gcatgactga taacaatacc gccctaaaga aagctggcct gaaagtaacg
    17581 cttcctcgtt taaaaatcct ggaagttctt caggagccgg acaaccatca cgtcagtgcg
    17641 gaagatttat acaaacgtct gatcgatatg ggtgaagaaa ttggtctggc tacggtatat
    17701 cgcgtactga accagtttga cgacgctggt atcgtcaccc gccacaattt tgaaggcggt
    17761 aaatccgtat ttgaactgac acagcaacat caccacgatc acctgatttg cctcgactgc
    17821 ggcaaggtta tcgaatttag tgatgattcc atcgaagcgc gtcagcgtga aattgccgca
    17881 aaacatggca ttcgcctgac taaccacagt ctctatcttt acggtcactg tgccgaaggc
    17941 gattgccgcg aagatgagca cgcgcacgaa ggcaaataag ccagcctgaa cgagaaaagc
    18001 caacctgcgg gttggctttt ttatacaagg gaaataagaa aagcgagagt tacagctctc
    18061 acttatttgt tattactgcg tatttcctgc cagaccttat cacaatcggc tttcaccgcc
    18121 tgatcatttc cggtttgcgt tgcacgcaga cactgctgat aatccagtac gcgtacactt
    18181 tcctggtcgg caaactgctg atgttgtttc tctttcttca gcacgtttaa cacgctctgg
    18241 caggcttcaa ttttttctgg cgaaccttgc gcggtgttga tacaggcgct gtatgcctct
    18301 ttcagacggc tgtcttcctg tggtgcggtt gattgcgcac aagctaccag ccccgatgcc
    18361 atcatggcga tgagaatcag ttttttcatt gaatgcggca ggcacaaaac ctgccgcgtc
    18421 gggcatcaga agatggtgaa tggtgcgatt accataaatt tcacgtcacg ctcatcctgg
    18481 aagatgttac cgtaaccgcc gccccagctt gggatgtcgg agtggttgtc gtattcggtg
    18541 aagtgcagtt tgaacatcgt gcctttggcg cgaccgtcct gaatggtgta gaccgcatcc
    18601 aggctgtagg cagactcttc aatagtccgg tttttgtcgt agtacgcatc cggattgctc
    18661 tgccaggtcg caggtttagc atcccatgcg taaacgtagg aagcgccgat ggcgaagcct
    18721 ggaagattcc agtttttcag gtcatacatc gcactgaaga agaccgcttt ttcgccgtta
    18781 gcgttgaagt cggaacggtt atcccaccag atatccaggc ggccgttgga ggaagcgtag
    18841 gttggggtca tacgttgcag gaagtatccc tgctgactgt cagccttaat ccaggtgcct
    18901 tcgaggcgca aatccactac gtcagccgcc cggtaaccaa aggtcaacgc ttgcagccag
    18961 gcggtgccgt cataaagatc gttgacgctg cgatcgtcaa ctttatcgcg ggtaccatag
    19021 aactggtagc tggtggttaa cgggctaccg gcgatatcaa atttgtagct ggctttggca
    19081 aaatattgat cgatataccc ttccgcctga ccaaatgccg cttccagtac gaagttattt
    19141 ttgaagtcgt atttcgcacc gatggagtgc agataatcaa ctttggtggt tttatcgttc
    19201 tgataaaact catccatttc cagatgccac ggcgctttgt attcgttggt ccacatgtag
    19261 gagaaactca aggcaccagc atcgccgtaa tcaaaattcg ccccggcttc cgccccctga
    19321 taagtacccg gcataaagct ccagtgcggc gctaacagcg tttgaccggt tggctgaatg
    19381 taacctgccc tcgcccaaac cggaccgtat ttaaatttgg ccgcagcttt atacaggctt
    19441 ataccgcttt tgtcgccgga ccagtcttcg tcataggctt tattactttt tgaaaacgcg
    19501 atttcgttcg ggtgggagct gtcgccgttt tccgccattt caatcgccgt aaacgcagca
    19561 atatcaaggc cgaacatatc agcagcataa ccggactgaa aatcgaggtt ggcattccag
    19621 gtggagtgag aaaggttggt tttgtatttg tcgccgtcgg taacatcttt acggtcgcgt
    19681 tcacgctgcc agtaatagat accgccggtt aaggttgaat cgtcgatgaa tccttcggcg
    19741 cgagcctccg gcattgccat aaagcccgac attgctgtaa caccggcaat agccagcgcc
    19801 agcgtactac gtttgccact aaacgtacgc atgggttaat cctctttgac gtataaattg
    19861 ctgcaccaaa ggtgaacagc gaaaaaaaat aaaaattaaa aaagtagcgt tgagaaaagt
    19921 ctcgctgact acaggaacag actatttttc gcgacgcgaa ttatcaatct ctttctgtaa
    19981 ccagaatgta agaacatgag caaccgcaca tattttgatg atttttgtta caaggcttaa
    20041 gtttgcggtt gaaaggcttg cagaagaata aaaacgtgaa attccagcaa gatgaaagcg
    20101 gttacatttt atttaattat gttttgtgat tttaaattta ttttatggat aaattgcgaa
    20161 gcggaattaa ttcgcgaaga aaagcagaaa aaacgccgca gagcgcggcg tttgggattg
    20221 tttgctattt cttgccggat gcggcgtgaa cgccttatcc ggcctacaaa agcacgcaaa
    20281 ttcaatatat tgcagagatc atgtaggcct gataagcgaa gcgcatcagg caatttagcg
    20341 tttgaaattt attgtctatt tattgccgga tgcgacgtaa atgcctcacc cggcttggta
    20401 gaacttaaaa ttactcgcct actttcgccc aggtatcacg cagcccaacg gtgcggttaa
    20461 ataccggttt ttcggccgtg gaatggcggc tgtcgaggca gaagtaacct tcacgctcaa
    20521 actggaatgc tttacccgca accgcatctt tcagcgacgg ttcagcaaag ccctgtttga
    20581 tcaccagcga ttccgggtta atcaccgaca ggaaatcatc cgcagcacct gggttaggca
    20641 cgctgaacag acgatcatac aaacggattt caaccggcag cgcatgtgcc gcgctcaccc
    20701 agtgaataac acctttgact ttacgaccat ctgccggatc tttgcttaag gtgtcggcgt
    20761 cataagtaca gaagatggtg gtgatattac cttcggcatc tttctccacg cgttcagcct
    20821 taatgacgta agcattacgc agacgcactt ctttacccag caccagacgt ttgtactgct
    20881 tgttagcttc ttcgcggaaa tcggcgcgat cgatccaaat ttcaccgcta aacggcacct
    20941 gacggctccc catttccggt ttgttcggat ggttcggcat ggtgaccatt tcgccttcgc
    21001 cctgatagtt ttcgataacc agtttcaccg gatcgataac cgccattgcg cgcggcgcat
    21061 tttcgttgag atcttcacgg atgcaggatt ccagcgacgc catctcaatg gtgttgtcct
    21121 gcttggtcac gccgatgcgt ttgcagaact cacgaataga agccgcagtg taaccacgac
    21181 gacgcagacc ggaaatggtc ggcatacgcg ggtcatccca gccttcaacg tgcttgtcgg
    21241 tcaccagcag gttcagctta cgcttggaca tcacagtgta ttccagattc aggcgcgaga
    21301 attcgtactg gcgcggatga acaggaatgg tgatgttatc cagtacccag tcgtacagac
    21361 gacggttgtc ctggaactca agcgtacaca gagagtgcgt aataccttcc agcgcatcgc
    21421 tgatgcaatg ggtgaagtcg tacatcgggt agatgcacca cttgttgcca gtctggtggt
    21481 gttcagcaaa tttaatgcgg tacagcaccg gatcgcgcat tacgataaat ggcgacgcca
    21541 tgtcgatttt cgcacgcagg caggctttac cttcttcaaa accaccggca cgcatttttt
    21601 cgaacagcgc caagttctct tcaacgctgc ggtcgcggta cgggctgttt ttacccggct
    21661 gcgtcagggt accgcggtat tcgcggatct gttccggtgt cagttcatca acgtacgcca
    21721 ggcctttatt gatcagttcg atcgcatagg cgtggagctg atcaaaataa tcggaggagt
    21781 aacggacgtt accagaccag tgaaaaccta accactctac gtcgtttttg atcgaatcga
    21841 catactcgat atcttctttt accgggttag tgtcgtcgaa acgcaggttg cactggcctt
    21901 tatagtcctg ggcgatcccg aagttcaggc agatagattt cgcatggcca atatgcagat
    21961 agccattcgg ctccggcggg aaacgggtgt gtactgtgat gtgcttacca ctggccagat
    22021 cttcatcgat gatctgacgg ataaagttag tcgggcgggc ttctgcctca ctcatcgtgg
    22081 attcctcaaa gcgtaaacaa cgtataacgg cgtatgatct tataagcggg acaggctgac
    22141 aactgttagt tttttaaaat tgataatcta acaaggatta tggggatgat tgatgaaaaa
    22201 aaaagcggca gagaagatct ccaccgcttg tcattgttgg atgcgacgct gccgtgtcgc
    22261 atcaggcata aagcagatta ctttttgatt tcatacagcg gtgtttgacc cgccacaaca
    22321 tggccctgag ctttaatgag caagccactg aaatcgtcga tattgctgca aaccaccggg
    22381 ctaatcatcg agcgggcgtt agcgttcagg taatccagat ccatttccag aatcggttgc
    22441 cctgcgctta cctgcgcacc ctcttccacc agacgtttaa agcctttacc ttccagcgct
    22501 acggtgtcga tacccatatg gacgacgatc tccgcgcctt tttcggtttc caggcagaac
    22561 gcgtggttgg tgttgaagat tttcacgatt gtccctgcgg ctggtgatac gacgatttta
    22621 tctgtcggtt tcaccgccac accgtcaccc accgctttgc tggcgaatgc ttcgtcagga
    22681 acctgatcca gtgccacgac atcaccggta atcggcgata ccagctccgc gatagatacc
    22741 gcgtttggta cagcctgcgg ttttgctaca ggcgcggcag ttgccggagt tgcttcagct
    22801 gacgcagcgg ctaccggacc acgggcaacg actttcttca tcgcatcgcc gatggattct
    22861 gctttcgcgc caacaatcac ctgaatagtc tgtttgttca gtttcactac accagaagca
    22921 cccagacgtt tacacatcgt atcgttgacg cgggcagagt cagccacggt caggcgcaga
    22981 cgggtaatac aggcatcaat cgctttcagg ttgtcagtac cgccaaccgc agcaatatag
    23041 ttggttgcca gttgagtcag accttcttca gtattgctgt tggcttcttc agtaacgatc
    23101 tcgtcttctt tatcttcacg acccggcgtt ttcaggttga acatgcggat aaccaaactg
    23161 aacaccacga agtagatagc gaagaagata acgcccatca ccagcagcat ccagacgttc
    23221 tggctggcgg caggcaggtt atacatcaac gcgtagtcga tagcacccgc agagaaagag
    23281 aagccggcat ggatacccag cagcgttgcc acaaacaggc tgataccggt cagcagtgcg
    23341 tgcaggaggt acagcagcgg cgcaaggaac atgaacagga attccagcgg ctcagtcaca
    23401 ccggtcagga acgcagtaac agcaacagaa agcagcatcc cgccaaccat cggacgacgc
    23461 tctttcggtg ctgcgaagta catcgccagc gccgcacccg gcagaccgaa catcatgatc
    23521 gggaagaagc cggacatgaa catccccgcg gtgccgtcac cagcgtagaa acggttgatg
    23581 tcgccgtgga aaaccgtacc cgccgcgttg gtgaattcac caatctggaa ccaggcgatg
    23641 gtgttcagca cctgatgcag accggttggg atcagcagac ggttgatgaa accaaagata
    23701 ccggaaccca gcgcgcccgc agaaacgatc cactcgccgc ctgcatggat agcgtgctgt
    23761 accggcagcc agacgtaacc aaaaatggcc gccagcacca gacagaagaa gccggtggcg
    23821 atcggcacaa agcgtttgcc gccgaagaag ctcaggaagt ccggcagttt aatatcggac
    23881 caacggttat aggctgcgcc accaaccaga ccggtaatga tacccgccag tacacccatg
    23941 ttaatttctg ggttgatggt caccatcgct ttggttaaca caaagtaacc taccgcaccc
    24001 gccagcgccg ccgcacctgc gctgtctttc gaccagctgg atgccacacc gatggcgaag
    24061 attaatgcga ggttatcaaa aattgcaccg cccgcctggg caataaacgc aacgttaagt
    24121 aaatctggct gaccgaatcg cagcaacagc gccgccaccg gcagcaccgc gatagggagc
    24181 tgtaacgccc taccgagtcg ctggaaaaaa cctaaaatat tcatcttatt ccccctacga
    24241 gaaccctatt tggctcgttt aaagccgtat ttttattttg ctgcaaattt tactgccgat
    24301 gttctgtaat cagattgtta gatcatctgc tacagagtgt gtgaaaattt aattcgcatc
    24361 gcaaattaaa cgcgtgtctt ttgtgagttt tgtcaccaaa tatcgttatc atcactccct
    24421 tttactggct aaaccagaaa acttatttta tcattcaaaa aatcaagtcg gattgacgcc
    24481 tgtctgcgca aatccaggtt acgcttaaag atgtctaatc cgccaacggc ttacatttta
    24541 cttattgagg tgaataatga gactgatccc cctgactacc gctgaacagg tcggcaaatg
    24601 ggctgctcgc catatcgtca atcgtatcaa tgcgttcaaa ccaactgccg atcgtccgtt
    24661 tgtactgggc ctgccgactg gcggcacgcc gatgaccacc tataaagcgt tagtcgaaat
    24721 gcataaagca ggccaggtca gctttaagca cgttgtcacc ttcaacatgg acgaatatgt
    24781 cggtctgccg aaagagcatc cggaaagcta ctacagcttt atgcaccgta atttcttcga
    24841 tcacgttgat attccagcag aaaacatcaa ccttctcaac ggcaatgccc cggatatcga
    24901 tgccgagtgc cgccagtatg aagaaaaaat ccgttcttac ggaaaaattc atctgtttat
    24961 gggcggtgta ggtaacgacg gtcatattgc atttaacgaa ccggcgtctt ctctggcttc
    25021 tcgtactcgt atcaaaaccc tgactcatga cactcgcgtc gcgaactctc gtttctttga
    25081 taacgatgtc aatcaggtgc caaaatatgc cctgactgtc ggtgttggta cactgctgga
    25141 tgccgaagaa gtgatgatta tggtgctggg tagccagaaa gcactggcgc tgcaggccgc
    25201 cgttgaaggt tgcgtgaacc atatgtggac catcagctgt ctgcaactgc atccgaaagc
    25261 gatcatggtg tgcgatgaac cttccaccat ggagctgaaa gttaagactt taagatattt
    25321 caatgaatta gaagcagaaa atatcaaagg tctgtaattg ttatccctgc cctggttcct
    25381 tgctcagggc aatatttttt aaaatcgggg ggcagaatgt atgcattaac ccagggccgg
    25441 atctttaccg gccacgaatt tcttgatgac cacgcggttg ttatcgctga tggcctgatt
    25501 aaaagcgtct gtccggtagc ggaactgccg ccagagatcg aacaacgttc actgaacggg
    25561 gccattctct cccccggttt tatcgatgtg cagttaaacg gctgcggcgg cgtacagttt
    25621 aacgacaccg ctgaagcggt cagcgtggaa acgctggaaa tcatgcagaa agccaatgag
    25681 aaatcaggct gtactaacta tctgccgacg cttatcacca ccagcgatga gctgatgaaa
    25741 cagggcgtgc gcgttatgcg tgagtacctg gcaaaacatc cgaatcaggc gttaggtctg
    25801 catctggaag gtccgtggct gaatctggta aaaaaaggca cccataatcc gaattttgtg
    25861 cgtaagcctg atgccgcgct ggtcgatttc ctgtgtgaaa acgccgacgt cattaccaaa
    25921 gtgaccctgg caccggaaat ggttcctgcg gaagtcatca gcaaactggc aaatgccggg
    25981 attgtggttt ctgccggtca ctccaacgcg acgttgaaag aagcaaaagc cggtttccgc
    26041 gcggggatta cctttgccac ccatctgtac aacgcgatgc cgtatattac cggtcgtgaa
    26101 ccgggcctgg cgggcgcgat cctcgacgaa gctgacattt attgcggtat tatcgctgat
    26161 ggcctgcatg ttgattacgc caacattcgt aacgctaaac gccttaaagg cgacaaactg
    26221 tgtctggtta ccgacgccac cgcgccagca ggtgccaaca ttgaacagtt catttttgcg
    26281 ggtaaaacaa tatactaccg taacggactt tgtgtggatg agaacggtac gttaagcggt
    26341 tcatccttaa ccatgattga aggcgtgcgt aatctggtcg aacattgcgg tatcgcactg
    26401 gatgaagtgc tgcgtatggc gacgctctat ccggcgcgtg cgattgacgt tgaaaaacgt
    26461 ctcggcacgc tcgccgcagg taaagtagcc aacctgaccg cattcacacc tgattttaaa
    26521 atcaccaaga ccatcgttaa cggtaacgag gtcgtaactc aataagagaa agtatgacac
    26581 caggcggaca agctcagata ggtaatgttg atctcgtaaa acagcttaac agcgcggcgg
    26641 tttatcgcct gattgaccag tacgggccaa tctcgcggat tcagattgcc gagcaaagcc
    26701 agcttgcccc cgccagcgta accaaaatta cgcgtcagct tatcgaacgc gggctgatca
    26761 aagaagttga tcagcaggcc tccaccgggg gccgccgcgc tatctccatc gtcaccgaaa
    26821 cccgcaattt ccacgcaatc ggcgtacggc ttggtcgtca tgacgccacc atcactctgt
    26881 ttgatctcag cagcaaagtg ctggcagaag aacattaccc gctgccggaa cgtacccagc
    26941 agacgctgga acatgccctg ctgaatgcca ttgctcagtt tattgatagc taccagcgca
    27001 agctgcgcga gctaatcgcc atttcggtga tcctgccagg gcttgttgac ccggacagcg
    27061 gcaaaattca ttacatgccg catattcagg tagaaaactg ggggctggta gaagcactgg
    27121 aagaacgttt taaagtgacc tgtttcgttg gtcacgatat ccgtagtctg gcgctggcgg
    27181 agcactactt cggtgcaagt caggattgcg aagactccat tctggtgcgt gtccatcgcg
    27241 gaaccggggc cgggattatc tctaacgggc gcatttttat tggccgcaac ggtaacgtcg
    27301 gtgaaattgg ccatattcag gtcgaaccgc tgggtgaacg ctgccactgc ggcaactttg
    27361 gctgcctgga aactatcgcc gccaacgctg ccattgaaca acgggtgttg aatctgttaa
    27421 agcagggcta ccagagccgt gtgccactgg acgactgcac catcaaaact atctgcaaag
    27481 ccgcgaacaa aggcgatagc ctggcctcgg aagtgattga gtatgtcggt cgtcatctgg
    27541 gtaaaaccat cgccattgct atcaacttat ttaatccgca aaaaattgtt attgctggtg
    27601 aaatcaccga agccgataaa gtgctgctcc ctgctattga aagctgcatt aatacccaag
    27661 cgctgaaggc gtttcgtact aatctgccgg tggtacgttc tgagctggat caccgctcgg
    27721 caatcggcgc ttttgcgctg gtaaaacgcg ccatgctcaa cggtattttg ctccagcatt
    27781 tgctggaaaa ttaatgtgct tttatagtgg cgcttattgt tgtcaatatt ctgggtagtc
    27841 catgaccatt aaaaatgtaa tttgcgatat cgacggcgtg ctgatgcacg ataacgtcgc
    27901 cgtaccgggt gcagcggaat ttttgcacgg gattatggat aaaggcctgc cgctggtgtt
    27961 gctgaccaac tatccttcgc agactgggca agatctggcg aaccgctttg ccactgccgg
    28021 tgtcgatgtg ccggatagcg tgttttatac ctctgcgatg gcgaccgccg atttcctgcg
    28081 tcgctaggaa ggcaagaaag cgtatgtggt gggcgaaggc gcactgattc atgaactgta
    28141 caaagccggt ttcactatta ccgatgtgaa ccctgatttc gtgattgttg gcgaaacacg
    28201 ttcctacaac tgggacatga ttcataaagc agcctatttc gtcgctaacg gtgcacgctt
    28261 tatcgccacc aacccggaca cccacgggcg cggtttttat cccgcttgtg gcgcgttgtg
    28321 tgcagggatt gagaaaatct ccgggcgcaa accgttctat gttggtaagc ccagcccgtg
    28381 gatcatccgc gcggcattaa acaaaatgca ggcgcattcg gaagaaacag tgattgtcgg
    28441 cgataacctg cgtaccgata ttctggcggg cttccaggcc ggtctggaga cgattctggt
    28501 gctttctggt gtttcatcgc tcgacgatat cgacagtatg ccgttccgcc caagctggat
    28561 ttacccgtca gtcgctgaaa tcgacgttat ctgaatacca cccacgtctg cggacgtggt
    28621 tggctctgat gacaaacgcc aaactgcctg atgcgctacg cttatcaggc ctacgcagct
    28681 cctgcaatat attgaatttt catgcttttg taggccggat aaggcgttca cgccgcatcc
    28741 ggcatgaaca aatcgcactt tatcagcaat ttggcccccg taaaatataa ccattcaata
    28801 aaacccgcta aaaattatcg attcatcaat aaatacatcc tcacaccaga ccctttttca
    28861 ccaatcgaca atcaccatta cgttttttat ttttgcactg ccaaatagct tgcgcacagt
    28921 gctgctttgc ggcatttttt taaacaagca aacacaacaa gcaacaaata ccaggttaac
    28981 ggagaaggtt atgtgttcaa tttttggcgt attcgatatc aaaacagacg cagttgagct
    29041 gcgtaagaaa gcactcgagc tgtcacgcct gatgcgtcat cgtggcccgg actggtccgg
    29101 tatttatgcc agcgataacg ccattctcgc tcacgaacgt ctgtcaattg ttgacgttaa
    29161 cgcgggggcg caacctctct acaaccaaca aaaaaaccac gtactggcgg taaacggtga
    29221 aatctacaac caccaggcat tgcgcgctga atatggcgat cgttatcagt tccagacagg
    29281 gtctgactgt gaagtgatcc tcgcgctgta tcaggaaaaa gggccggaat ttctcgacga
    29341 cttgcagggc atgtttgcct ttgccctgta cgacagcgaa aaagatgcct acctgattgg
    29401 tcgcgaccat ctggggatca tcccactgta tatggggtat gacgaacacg gtcagctgta
    29461 tgtggcctca gaaatgaaat cgctggtgcc agtttgccgc acgattaaag agttcccggc
    29521 ggggagctat ttgtggagtc aggacggcga aatccgttct tattatcatc gcgactggtt
    29581 cgactttgat gcagtgaaag ataacgtgac cgacaaaaac gagctgcgtc aggcactgga
    29641 agattccgtt aaaagccatc tgatgtctga tgtgccttac ggtgtgctac tttctggtgg
    29701 tctggattcc tcaattattt ccgctatcac caagaaatac gcagcccgtc gcgtggaaga
    29761 tcaggaacgc tctgaagcct ggtggccgca gttacactcc tttgctgtag gtctgccggg
    29821 ttcaccggat cttaaagcag cccaggaagt ggcaaaccat ctgggcacgg tgcatcacga
    29881 aattcacttc actgtacagg aaggtctgga tgccatccgc gacgtgattt accacatcga
    29941 aacttatgat gtgaccacta ttcgcgcttc aacaccgatg tatttaatgt cgcgtaagat
    30001 caaggcgatg ggcattaaaa tggtgctgtc cggtgaaggt tctgatgaag tgtttggcgg
    30061 ttatctttac ttccataaag cgcccaacgt taaagaactg catgaagaga cggtgcgtaa
    30121 actgctggcc ttgcatatgt atgactgcgc gcgcgccaac aaagcgatgt cagcctgggg
    30181 cgtggaagca cgcgttccgt tcctcgacaa aaaattcctt gatgtggcga tgcgcattaa
    30241 cccgcaggat aaaatgtgcg gtaacggcaa aatggaaaaa cacatcctgc gtgaatgttt
    30301 tgagtcatac ctgcccgcaa gcgtggcctg gcggcagaaa gagcagttct ccgatggcgt
    30361 cggttacagt tggatcgaca ccctgaaaga agtggcggcg cagcaggttt ctgatcagca
    30421 actggaaact gcccgcttcc gcttcccgta caacacgccg acctcaaaag aagcgtatct
    30481 gtaccgcgag atctttgaag aactgttccc acttccgagt gccgctgagt gcgtgccggg
    30541 cggtccttcc gtcgcgtgtt cttcagctaa agcgatcgaa tgggatgaag cgttcaaaaa
    30601 aatggacgat ccgtctggtc gtgcggttgg cgttcaccag tcggcgtata agtaagaaat
    30661 gcggtacagc cccggagaaa tccggggctg ttttagttat tgcgctgctt gttgattctg
    30721 atatgcagta aggaaatgtt ccgggtttgc cacatcactg tttgacaact catcactgta
    30781 ggccagtatt tcatagatta atttcccacc actattgttg aattccgaat ttcatcctgt
    30841 acgacaaaag aatggctgta gggtggggaa aaacacaaag taaacaataa ttgacgaata
    30901 tagcgccacg ctgttcgcaa cctaaccaaa cagtcacttt cgagcaattt tccttgaaaa
    30961 agaggttgac gctgcaaggc tctatacgca taatgcgccc cgcaacgccg ataaggtatc
    31021 gcgaaaaaaa gatggctacg tagctcagtt ggttagagca catcactcat aatgatgggg
    31081 tcacaggttc gaatcccgtc gtagccacca tcttttttgc gggagtggcg aaattggtag
    31141 acgcaccaga tttaggttct ggcgccgcaa ggtgtgcgag ttcaagtctc gcctcccgca
    31201 ccattcacca gaaagcgttg tacggatggg gtatcgccaa gcggtaaggc accggttttt
    31261 gataccggca ttccctggtt cgaatccagg taccccagcc atcttcttcg agtaagcggt
    31321 tcaccgcccg gttattgggg tatcgccaag cggtaaggca ccggtttttg ataccggcat
    31381 tccctggttc gaatccaggt accccagcca tcgaagaaat aatctggcta cgtagctcag
    31441 ttggttagag cacatcactc ataatgatgg ggtcacaggt tcgaatcccg tcgtagccac
    31501 caaattctga atgtgtcgaa tacgttcggc aaattcaaaa accaatttgt tggggtatcg
    31561 ccaagcggta aggcaccgga ttctgattcc ggcattccga ggttcgaatc ctcgtacccc
    31621 agccaattta ttcaagacgc ttacatcgta agtgcaccca gttggggtat cgccaagcgg
    31681 taaggcaccg gattctgatt ccggcattcc gaggttcgaa tcctcgtacc ccagccacat
    31741 taaaaaagct cgcttcggcg agctttttgc ttttctgcgt tcattcaatg tcgaatgcga
    31801 tgttgacacg tcttatcctt caatgtcgga tgcgacgctg ccgcgtctta tccgacctac
    31861 ggctggcacg catccggcaa tgttgtaagg ctacaaccct aacgcatatt tcagcgcctg
    31921 acgtttcaac acgccagcac gctctgccgc cattaacccg agattacgca caaaacgcag
    31981 cggcggcaga ttattactga atccggcata aaacagatcc ataccacttt gcataatgaa
    32041 gttatccgcc atgcgccgca tctggtaacg cttgaggaca ggataactgg cccacgcttc
    32101 accgtagctg cgggcattca ccagaacatc aatcagggca tcgacatcac gataaccaag
    32161 attcaccccc tgccccgcca gcggatggat ggtatgcgcg gcatcgccca ccagcgcaag
    32221 ccccggctgc acatactgca acgcatgtcg gcgcgtcagc ggaaacgcac cagcggcaag
    32281 cggtgtaacg taacccagac gcgacgggaa atgcttcgcg atttccgcct ggagttgcgc
    32341 catattcata ttctgcaact ggcgaatacg cgtcggagag tcataccaca ccagcgatgc
    32401 ccagttatca aacaacggca gaaacgcacg cggtccgtcc ggggtaaatt gctgccaggt
    32461 gctgtcgccg ggatcgttct cacactgtac gctaatcaac atgcacgact gcgcatactg
    32521 ccaggcatga acgccaattc ccgccatctg ccgcacctga gaatttgcgc cgtcggcacc
    32581 aatcaccagt ttcgcgcgga tcgtttcacc gcccttcagt tccagctcct gaagatcatt
    32641 atctcgatgt aatgcaatca gcgaggttgg cacacgtaac gttactttcg gatgcgcttc
    32701 cagcgcctgc cacaacgcct gttgcaggac tgtgttttcc accatgtagc caagcaacgg
    32761 caactttaat tcggctgcgt caaataccac atgtgccgtt tcccactccc aggtttccag
    32821 tctgcggtaa ggatggcaac gcatagcctg tactgcatcc cagaccccta accctttaag
    32881 caatgatacc gaagccgcgc tgatcgccga gatccgcacg tccggttggc tgtcagcgac
    32941 aaacggtgca ggttctgcgt gctcgatcac cgttaccgaa aatccgtgct gtgccagccc
    33001 cagcgccagt gcgccgccga ccattcctcc gccgacaatg gcaatttccg ttggttgatt
    33061 tgtcatggtc aatcatcctg ttagcaatat gctttaagtt tacaggattt ttacccccaa
    33121 gggctgataa cgctcatact ggtcacaacg gcagcaaagc attacactat gcgccctgca
    33181 ttcctggcta ctatttcgca agagcaagtc gatgaccaaa aaactccata ttaaaacctg
    33241 gggctgtcag atgaacgagt acgattcatc gaagatggcc gatctgctgg atgccaccca
    33301 cggctatcaa ctgaccgacg tggcggaaga agcggatgtg ctactgctga acacctgctc
    33361 aatccgcgag aaggctcagg aaaaagtctt ccatcagttg ggtcgctgga aactgttaaa
    33421 agagaagaat ccagacctga ttatcggcgt cggtggctgc gtggcatcgc aagaaggcga
    33481 gcacattcgc cagcgcgccc actatgtcga tattattttt gggccgcaaa cgctgcaccg
    33541 tctgccggag atgatcaact ccgtgcgcgg cgaccgcagc ccggttgtag atatcagctt
    33601 cccggaaatc gagaagtttg accgtctgcc ggaaccgcgc gccgaagggc cgaccgcgtt
    33661 tgtctccatc atggaaggct gcaataaata ttgcacctac tgcgtggtgc cttacacccg
    33721 tggtgaagag gtaagccgtc cgtctgacga tattctgttt gagattgccc agcttgcggc
    33781 tcagggcgtg cgtgaagtca acctgctcgg tcagaatgtg aatgcctggc gtggtgagaa
    33841 ctacgacggc accaccggat cgtttgccga tctgttgcgt ctggttgctg cgatcgacgg
    33901 gatcgatcgt attcgcttta ccaccagcca tccgatcgaa ttcaccgacg atatcatcga
    33961 agtgtaccgc gacacgcctg agctggtgag cttcctgcat ctgccggtac agagcggttc
    34021 cgatcgcatt ctgaacctga tgggacgtac ccatacggcg ctggagtaca aagcgatcat
    34081 ccgtaaactg cgtgcggcgc gtccggatat tcagatcagt tctgacttca ttgttggctt
    34141 ccctggcgaa accaccgaag acttcgagaa aacgatgaag ctgattgccg acgtcaattt
    34201 cgacatgagc tacagcttta tcttctctgc acgtccagga acgcctgccg ccgatatggt
    34261 tgatgatgtt ccggaagaag agaagaagca gcgtctgtat attctgcaag aacgtatcaa
    34321 tcagcaagcg atggcatgga gccgtcggat gctcggcacc acccagcgta ttctggtaga
    34381 agggacttcg cgtaagagca tcatggagct ttccgggcgt accgaaaata accgcgtggt
    34441 caacttcgag ggcacaccgg atatgatcgg taaattcgtc gatgtagaaa ttaccgacgt
    34501 ctacccgaac tctctgcgcg gtaaagtggt gcgaactgaa gatgaaatgg gtctgcgcat
    34561 ggcagaaaca ccggagtcgg tgattgcccg cacccgcaaa gaaaacgacc ttggcgtggg
    34621 ttattatcag ccgtaattct caggcctgcc gttccggtgg gccttgtttt ttctctcttt
    34681 atccccatct ttatcgcaat gcttgcgcct ttttcctgtg gcgtgaataa tttccttata
    34741 ggccggtgaa tgttcagctt ccggcggcat acagataaga ggaacggttt gaacatagac
    34801 actcgcgaaa tcaccctgga gccagcagac aatgcgcgtc tgttgagcct gtgcggcccg
    34861 tttgatgaca acatcaagca gctcgaacgc cgtctcggca tcgagatcaa tcgccgcgat
    34921 aaccacttta aactgaccgg ccgtccgatt tgcgtcaccg ctgcggcaga cattctgcgt
    34981 agcctgtatg tcgatactgc cccgatgcgc ggtcagattc aggatatcga accggaacag
    35041 atccaccttg cgattaaaga agcgcgggta ctggagcaaa gcgcggagag cgtgccggag
    35101 tacggcaaag cggtcaatat caaaactaaa cgcggcgtaa ttaagccgcg cacgccaaac
    35161 caggcgcagt acatcgccaa tattctcgac catgacatta ccttcggcgt tggcccggcg
    35221 ggtacgggta aaacctacct ggcagtggct gcggcagttg atgccctgga gcgtcaggaa
    35281 attcgccgta ttctgctgac tcgtccggcg gtcgaagccg gtgagaaact gggcttcctg
    35341 cctggcgatt taagccagaa agtagacccg tatctgcgcc cactgtacga cgcgctgttt
    35401 gaaatgctgg gctttgagaa agtcgagaaa ctgattgagc gcaacgttat tgaagtcgcg
    35461 ccgctggcct atatgcgtgg tcgtacgctg aacgacgcgt ttatcattct cgatgagagc
    35521 cagaacacta ccatcgaaca gatgaagatg ttcctgaccc gtatcggttt taactcaaaa
    35581 gcggttatca ccggcgacgt cacacagatc gacctgccgc gtaatactaa atcaggctta
    35641 cgtcatgcca ttgaagtgct ggccgatgtc gaagagatca gctttaactt cttccacagc
    35701 gaagacgtgg ttcgtcaccc ggtggtggcg cgtatcgtta acgcttatga agcctgggaa
    35761 gaagcagaac aaaaacgtaa agcggcgctg gcagcagaac gcaagcgcga agaacaggaa
    35821 caaaaatgag tcaggtgatc ctcgatttac aactggcatg tgaagataat tccgggttac
    35881 cggaagagag ccagtttcaa acatggctga atgcggtgat cccgcagttt caggaagagt
    35941 cggaagtgac tattcgcgtg gtcgataccg ccgaaagcca cagtctgaat ctgacctatc
    36001 gcggtaagga taagccgacc aacgtgctct ccttcccgtt tgaagtgccg cctggcatgg
    36061 agatgtcgct actgggcgat ctggttatct gccgtcaggt ggttgagaag gaagctcagg
    36121 agcaaggcaa accactggag gcgcactggg cgcatatggt ggtgcacggc agtctgcatt
    36181 tgttaggtta cgatcacatt gaagatgacg aagcagaaga aatggaagcc ctcgaaacag
    36241 agattatgct tgctctgggc tatgaggatc cgtacattgc cgagaaagaa taatttgcca
    36301 ggcatttgac tggcagacgt ccccgccgtt ggtgattaac gacggcgttt tgattaacta
    36361 acatgagaac ccatacgacg ccatgagcga cgacaattca cacagtagtg acacgataag
    36421 caacaagaag ggatttttct ccctgttact cagccaactt ttccacggtg aaccgaaaaa
    36481 ccgtgacgaa ctgctggcgc tgatccgtga ttccgggcag aacgacctta tcgacgaaga
    36541 tacgcgcgat atgctcgaag gggtgatgga catcgcagac caacgcgttc gcgacatcat
    36601 gatcccccgc tcccagatga ttaccctgaa acgcaaccag acgctggacg aatgccttga
    36661 tgtcatcatc gagtccgccc actcacgttt cccggtgatt agcgaagaca aagatcacat
    36721 tgaagggatt ctgatggcga aagacttgct gccgtttatg cgcagcgatg ctgaagcctt
    36781 cagcatggac aaagtgttac gtcaggcggt tgtcgttcct gaaagtaagc gcgtagaccg
    36841 gatgctgaaa gagtttcgct ctcagcgtta ccacatggcg atcgttattg acgaattcgg
    36901 tggggtttcc ggtctggtga ccattgaaga catcctggaa ctgattgttg gtgagattga
    36961 agacgagtat gacgaagaag atgatatcga cttccgtcag ctgagtcgtc atacctggac
    37021 cgtgcgcgca ctggcttcca ttgaagactt caacgaagcg ttcggcaccc actttagcga
    37081 tgaagaagtc gacactatcg gtggtctggt gatgcaggcg tttgggcatc ttccggcgcg
    37141 tggcgaaact atcgacatcg acggttacca gttcaaagtg gcgatggccg atagtcggcg
    37201 tattattcag gttcatgtca aaatcccgga tgactcaccc cagccaaagc tggatgaata
    37261 aaaccgaaac tggatagata actacatgga ttttgcctca ttaattgaac gccagcgcat
    37321 tcgcctgctg ctggcgttat tattcggtgc ctgcggaacg ctggccttct ctccttacga
    37381 cgtctggcct gcggcgatta tttcgctgat ggggcttcag gcgttgacct ttaaccgtcg
    37441 cccactccag tctgccgcta ttggcttttg ctggggattt ggcctctttg gcagcggtat
    37501 taactgggtc tatgtcagca tcgcgacctt tggcgggatg cctggcccgg ttaacatctt
    37561 cctggtggtg ctgctggcgg cgtatttgtc gctgtatacc ggactgtttg ctggcgtgct
    37621 gtcgcgtctg tggccgaaaa ccacctggct gcgcgtggcg attgccaccc ctgctctctg
    37681 gcaagtgacc gagtttctgc gcggttgggt gctgactggc ttcccgtggt tacagttcgg
    37741 ctatagccag attgatggcc cgttaaaagg gctggcaccg ctaatgggcg tggaagccat
    37801 taacttcctg ctgatgatgg ttagtggcct gctggcactg gcgttggtca aacgcaactg
    37861 gcgtccgctg gtggtggccg tcgtgctgtt tgccctaccc ttcccgctgc gttacatcca
    37921 atggtttacc ccgcaaccgg agaaaaccat tcaggtttcg atggttcagg gcgatattcc
    37981 gcaatcgctg aaatgggacg aaggccagct tcttaatacg ctaaagattt actacaacgc
    38041 aacggcaccg ctgatgggca aatcatcgtt gattatctgg ccggagtcgg cgataaccga
    38101 tctggaaatt aatcagcaac cgttcctcaa agcactggac ggtgagttgc gtgataaagg
    38161 tagctcgctg gtaaccggga ttgtcgacgc gcgtctcaat aagcagaacc gctacgatac
    38221 ctacaacacc atcatcacgc tgggtaaagg tgcaccgtac agctacgaat cagccgatcg
    38281 ctataacaaa aaccatctgg tgccgtttgg cgagtttgtc ccgctggagt cgattctgcg
    38341 tccgttagca ccgttctttg atctgccgat gtcgtcgttc agccgtgggc catatatcca
    38401 gccgccgctg tcggcaaatg gtattgagct tactgcggct atttgctacg agattattct
    38461 cgccgagcaa gtgcgcgata acttccgccc ggataccgac tacctgctga ctatctccaa
    38521 cgatgcgtgg tttggtaaat ctattggtcc atggcaacac ttccagatgg cgcgaatgcg
    38581 tgcgctggag ctggcgcgcc cactgttgcg cagcaccaac aacggcatta cggcggtgat
    38641 tggcccgcag ggtgagattc aggcgatgat cccgcagttc gcccgcgagg tgttaaccac
    38701 taacgtgacg ccgaccaccg gactcacacc atacgcacgt accggcaact ggccgctgtg
    38761 ggtgctgacg gcactgtttg gttttgctgc tgtgttgatg agtctgcgtc agcgacgtaa
    38821 ataatccctt cttatcgtgc cggaatacat cccatatttc ggcacgctca ccggatgcgg
    38881 catcagtgca ttatccggat aacaatattc tcctatccaa ctttggcaca tctattgctt
    38941 tgttatacaa ggcaaagctt gaaccagcat cagtacaacc cggttgcacc acagcaatcc
    39001 accggtagca ccaaaagagt gcattttaag aattttgctt cagaatggtg cggtgcacct
    39061 cgcaaaaaca aacaataaaa catcaatatc tttacattta gccaaactaa atgttaccta
    39121 acaggcacaa cactgcacaa taaagttgca gacgataaca acacaaacac tcacaacggg
    39181 tatccatgcg ttcttaacgc agaagataaa ggtgttggat atgcaattac gtaaacctgc
    39241 cacagcaatc ctcgccctgg cgctttccgc aggactggca caggcagatg acgccgcccc
    39301 ggcagcaggc agtacgctgg acaaaatcgc caaaaacggt gtgattgtcg tcggtcaccg
    39361 tgaatcttca gtacctttct cttattacga caatcagcaa aaagtggtgg gttactcgca
    39421 ggattactcc aacgccattg ttgaagcagt gaaaaagaaa ctcaacaaac cggacttgca
    39481 ggtaaaactg attccgatta cctcacaaaa tcgtattcca ctgctgcaaa acggcacttt
    39541 cgattttgaa tgtggttcta ccaccaacaa cgtcgaacgc caaaaacagg cggctttctc
    39601 tgacactatt ttcgtggtcg gtacgcgcct gttgaccaaa aagggcggcg atatcaaaga
    39661 ttttgccgac ctgaaaggca aagccgtagt cgtcacttcc ggcactacct ctgaagtttt
    39721 gctcaacaaa ctgaatgaag agcaaaaaat gaatatgcgc atcatcagcg ccaaagatca
    39781 cggtgactct ttccgcaccc tggaaagcgg tcgtgccgtt gcctttatga tggatgacgc
    39841 tctgctggcc ggtgaacgtg cgaaagcgaa gaaaccagac aactgggata tcgtcggcaa
    39901 gccgcagtct caggaagcct acggttgcat gttgcgtaaa gacgatccgc agttcaaaaa
    39961 gctgatggat gacactatcg ctcaggtgca gacctccggt gaagcggaaa aatggtttga
    40021 taaatggttc aaaaatccaa ttccgccgaa aaacctgaac atgaatttcg aactgtcaga
    40081 cgaaatgaaa gcactgttca aagaaccgaa tgacaaggca ctgaactaat tacaagaacc
    40141 aggggcggaa attccagccc tctcgattgt tacgtagcac ggacagacta tacgcctgat
    40201 ggtcgttccc catcgggcct gaaaaccgca atacgctggg taacaatctt cgagggtagc
    40261 agttaacgct gctaccctct tttttctgga gtagatttat gtctatagac tggaactggg
    40321 gtattttttt acaacaagcc ccgttcggca ataccaccta tcttggttgg gtctggagtg
    40381 gttttcaggt gacgatcgct ttgtcgatct gcgcctggat tatcgctttc ctcgtcggtt
    40441 catttttcgg cattttacgt accgttccaa accgttttct ctccggtctg ggtacgttgt
    40501 atgtcgaact gttccgcaac gtgccgttga ttgtgcaatt ctttacctgg tatctggtga
    40561 tcccggagct gctgccggag aaaatcggca tgtggtttaa ggccgagctg gatcccaata
    40621 ttcagttttt cctttcatcc atgctctgcc tggggctgtt taccgccgcc cgtgtttgcg
    40681 aacaggtacg cgccgctatt cagtcgctac cgcgtgggca gaagaatgcc gcgctggcga
    40741 tgggcctgac gctaccgcag acttatcgat acgtactgct gcctaatgct tatcgcgtta
    40801 tcgtcccgcc gatgacttcc gagatgatga acctggtgaa aaactccgcc atcgcctcga
    40861 ccatcggtct ggtggatatg gcggcgcagg cgggtaaatt gctcgactac tcggctcacg
    40921 cctgggagtc gtttaccgcc atcacgcttg cgtatgtgtt gattaacgct tttatcatgc
    40981 tggtgatggc gctggttgaa cgtaaagttc gcctgcccgg caacatgggg ggcaaataat
    41041 gtacgagttt gactggagtt ccattgttcc ttccctgcca tatctgctcg acgggctggt
    41101 gatcaccctg aaaatcaccg tcacggcagt cgtgattggt attttgtggg gtaccatgct
    41161 ggcggtgatg cgcttgtcca gcttcgcgcc tgtttcgtgg tttgccaaag cctacgttaa
    41221 cgtattccgc tcgattcctt tagtcatggt tttgctgtgg ttttacttga ttgtgccggg
    41281 atttctgcaa aatgtgctgg gattatcgcc aaaaaatgat attcgtttaa tttcggcgat
    41341 ggtggcgttt tcgatgtttg aagcggccta ctattcagag attatccgtg ccggtattca
    41401 aagtatttcc cgtggtcagt cgagcgccgc gctggcgttg gggatgactc actggcagtc
    41461 gatgaaactg attattctgc cgcaggcttt ccgcgcgatg gtgccgctgc tgctcactca
    41521 gggcatcgta ctgttccagg atacctcgct ggtgtatgtg ttatctctgg ccgatttctt
    41581 ccgtaccgcc tcaaccattg gtgagcgtga tggtacgcag gtcgagatga tcctgtttgc
    41641 cggatttgtt tatttcgtta ttagccttag cgcgtcgttg ttggtcagct acttgaaaag
    41701 aaggacagca taatgattac cctgaaaaat gtttcaaaat ggtatggtca ctttcaggtg
    41761 ctgaccgact gctcaaccga agtgaaaaaa ggcgaagtgg tggtggtttg cggcccgtct
    41821 ggttccggca aatcaacgct gattaaaacc gtcaacggcc tcgaaccagt gcagcaaggt
    41881 gaaatcaccg tcgatggtat cgtggttaac gacaagaaaa ccgatctggc aaagctgcgc
    41941 tcccgcgtcg ggatggtatt ccagcatttc gagctgttcc ctcatctgtc gattatcgaa
    42001 aacctgaccc tggcgcaggt gaaagtgctt aaacgcgata aagcgcccgc tcgtgaaaaa
    42061 gcattgaaat tgctggagcg cgtgggactt tccgcacatg ccaataaatt tccggcgcag
    42121 ctttccggcg gtcagcagca gcgcgtggca atcgctcgcg cattgtgtat ggctcctatt
    42181 gcgatgctgt tcgacgaacc gacatcggcg ctggatccgg agatgatcaa cgaagtgctg
    42241 gacgtgatgg tggaactggc gaacgaagga atgaccatga tggtggtgac ccacgaaatg
    42301 ggctttgccc gcaaagtggc gaatcgggtg atctttatgg acgagggtaa aattgtcgaa
    42361 gactcgccga aagacgcttt cttcgatgat ccgaaatcgg accgcgcaaa agacttcctc
    42421 gcgaaaatcc tgcattaatc actctggcgc acatacccaa ttgcgcgcca tctcacgctt
    42481 tatcgtttcg cccctcgtat ctgttacatc ctggcgttac ccttgtcgca aagaagcaca
    42541 caacaaggag caacaatggc actgccaatt ctgttagatt gcgacccagg tcatgatgac
    42601 gctatcgcaa tagttctcgc actcgcctca ccagagcttg atgtcaaagc aattacgtct
    42661 tccgccggaa accagacacc agaaaaaacc ttacgcaatg ttctgcgtat gctgaccttg
    42721 cttaatcgca ccgatattcc ggtggcaggc ggcgcggtaa aaccgttaat gcgtgagttg
    42781 attatcgcgg acaatgtgca cggcgaaagc ggtctcgacg gcccggcatt accggaaccg
    42841 acgttcgcac cgcaaaactg tacggcggtg gagctgatgg cgaaaacgct gcgcgaaagt
    42901 gcggaacctg tcacgattgt ttctaccgga ccgcaaacta acgttgcctt gctgctcaat
    42961 agccatccgg aactgcatag caaaattgcc cgtatcgtga ttatgggcgg cgcaatgggg
    43021 cttggtaact ggacgcctgc ggctgaattt aatatttacg ttgacccgga agcggcagaa
    43081 attgtcttcc agtcagggat cccggtggtg atggccggtc tggatgttac tcataaagcg
    43141 caaatccacg ttgaagacac cgagcgtttc cgcgcgattg gcaaccctgt ttcaaccatt
    43201 gttgccgaac tgctggattt cttcctcgaa tatcataaag acgaaaaatg gggctttgtc
    43261 ggcgcaccgc tgcatgaccc atgcaccatc gcctggctgt tgaaacccga gttgtttacc
    43321 actgttgagc gctgggttgg cgtggaaaca caggggaaat atacccaggg tatgacggtt
    43381 gttgattatt attatctgac tggcaataaa ccgaatgcca ctgtaattgt cgatgttgat
    43441 cgtcagggct ttgttgattt actggctgat cgtctgaaat tttacgctta acactcgtca
    43501 ttgattaaga tcaccacgcg ttacctgggt ggtcttaatc tatttatctc tttttctttc
    43561 aagatataaa tgcaatggat aatgcagaac tcgccatagg tatcgatctc ggcactacca
    43621 atagtttaat tgccgtctgg aaagacggtg ccgcgcaatt aattccaaat aagttcggtg
    43681 aatatttaac accatccata attagcatgg atgaaaataa tcatatttta gtcggaaaac
    43741 cggctgtatc acggcgtact tcgcatccgg ataaaacggc agcgttattt aaacgtgcaa
    43801 tgggcagtaa taccaactgg cggttaggca gcgacacatt taacgcgcca gaactgtcct
    43861 ctttggtatt acgctcatta aaagaagatg ccgaagaatt tctgcaacgt ccgattaaag
    43921 atgtggtgat ctccgttctg gcttatttca gcgatgaaca acgcaagcat acccgtttag
    43981 cagcggactt agccgggtta aatgcggtac gcttaattaa tgaacccaca gcagctgcga
    44041 tggcgtatgg cctgcatacc caacaaaata cccgttcgct ggtgtttgat ctcggtggcg
    44101 gcacgtttga cgttacggtg cttgagtacg ccacgccggt gattgaagtt cacgcctccg
    44161 ctggcgacaa cttcctcggt ggcgaagatt ttacccatat gttggtcgat gacgttttaa
    44221 agcgcgcgga tgtcacaagg accacgctta acgagagtga actggcagcc ttgtacgcct
    44281 gtgtggaagc ggcaaaatgt agcaatcaat cgccaatgca cattcgctgg tagtaccagg
    44341 atgaaacgcg ggaatgcgaa ttttacgaga acgaactgga agatttgtgg ttgccgctgc
    44401 tcaatcgctt gcgagtgccg attgaacagg cgttgcgcga tgcgcgtctg aagccgagtc
    44461 aaatcgacag tctggtgctg gttggcggcg cgtcacaaat gccgctggtg cagcgaatcg
    44521 ccgtgcgtct gtttggcaaa ttaccgtatc aaagttacga tccgagcacc attgtcgcgc
    44581 tgggcgcagc aatccaggcc gcctgccgct tacgcagtga agatattgaa gaagtgatcc
    44641 tcactgatat ttgcccttac tcgttgggcg ttgaggttaa ccgccagggc gtttccggca
    44701 ttttctcgcc gattattgaa cgaaacacca ctgtgcccgt gtcgcgtgta gaaacttatt
    44761 caaccatgca cccggaacag gattcaatta cggttaacgt ctatcaggga gaaaaccaca
    44821 aagttaaaaa caacattctg gtggaatcct tcgatgtgcc gttgaagaaa accggggctt
    44881 atcagtcgat tgatattcgc tttagttatg atatcaacgg gttgcttgaa gttgacgtgc
    44941 ttctggaaga cggcagcgtt aagtccagag tgattaacca cagcccggta acattgagcg
    45001 cgcagcagat tgaagagagt cggacgcggt tatccgcatt gaaaatttat ccgcgcgata
    45061 tgctcatcaa tcgcaccttt aaagccaaac tggaagagtt gtgggcgcgg gcgctgggtg
    45121 acgagcgaga agagattggc cgggtgatca ccgattttga tgcggcgctg cagtcaaacg
    45181 atatggcccg cgtcgatgag gtcaggcggc gggcgagcgt ttatttagcc attgagacct
    45241 cataatcacg gctgccggat gcggcgtaaa tgctgcatcc ggcatcggcg caaaaaacac
    45301 atcgtcaact aaacaacatc gcgattttgc cgagagcata acctataacc agcaccgtta
    45361 aactgatttt gataatgctg gtaatgctct gtggtttttc accctcatca tttttttctt
    45421 catggttttc aggtgttggc tcatcgacat cttcatcctc cggctcgagt tttactttcg
    45481 tcaacagcat ccgggtgata atggcctcta atggatgagc atctccgcct tccagccttg
    45541 tattaagacg ggccagcatc tcttgtttcg gaaacgcttc aagtactacc tgttccgcta
    45601 cccgatacaa atccagtaac ggtgaatctt cttctgtctc gctaccgact ctcgatttcc
    45661 accacgccca tgctttgcgt ttgaagaata acgactcaat aaacgattga cctaacggcg
    45721 ataattttcc gctatgtaaa atttccgccc ataacagccg cgtccgggcg ctctcaccag
    45781 gaacatattt cagcggttga ccttgcgcat ctaccatgct ccgcgcttcg ataaccatca
    45841 ctaataacgc gaaataatcc gggcaatgac gcttgctcca ctgcaacaac aaattatcag
    45901 cttgtgtaga tgggtaactt tcccagtacg cgccgagatc agcaagcagg ctttccccct
    45961 cgccgcagta caaacgttgt gcacactggt aataccgggc atcttcgctt tctggttctt
    46021 ccgcttgcca ctgtttggct acaggaatga gttccaccat acctgtttgc accgagctgt
    46081 accagcgcaa cagtttgcga tgtaactttg catcatcagg aatgaaccac ggtccttcca
    46141 tcgccaaaaa cgcagtgaca tagtcaggat ggtaacggca gcaacgttcc agcgcgaagt
    46201 aatattcaac agtctggttc tgtaacgcga cgggatattc cagaaggcta aggaaatcga
    46261 aacaatcacc agcctgaatg gcctcaagaa aagcctccct ttcttcctca tccattcctt
    46321 cgctgtcatt aaacgaatgc cagttcaaac gctctgccaa caaagaagca caatttaatg
    46381 acagatatcg cgcttctata gcgattgcac acagcggcca gcgtaattga tcgacatcct
    46441 ccatgttcca ggtatttaat tgctgaataa atttctgcca ggcggagggc tgaaaacgat
    46501 cactttctga atccagtaat gtcctgaatg cacgtagtat ttcatgttca gcggtggcat
    46561 ctttttcttc atcatctgct tcctcgacag gatttaccgc cagtcgcagc gcctcttcat
    46621 aggcctggcg taatgcttta aacccctgcg gatcggtttc gggatgacac aacggtaagc
    46681 gagcaagata agcctggcgg ataatgtcta tttgcgtcgt gctttcaatt tcgaggattt
    46741 gccaacatgt tttcataggt cgctcacatc cttaagcgtt accgggcatt caggctggac
    46801 agttaatggc tgccagggaa gcgtggcata gaaacggtcg cttccagctg ccagctcttc
    46861 gaacatccga ctcccggcgc tatcgtattt gtacagggtt agcgcctcgc gtagcgaagc
    46921 gttgtcttcg cactgagaag attgccagta gagacgccct agtgaatagg cagaaaaata
    46981 gtgcatccag ctgtgatagt aatggtgagc tcgtgcatag acgcgcgatt gcagccataa
    47041 actctcttct tccgtcagcc agttgttgag cacaccggcc cgtgacagaa atcccattcg
    47101 aacataatcc caggctttga ttcccccttc tccacagcac atagcggttt gggcaacgaa
    47161 ttgggtataa atcaaccctc tctccgagcc accagcaatt aatgctttcc actcttcagg
    47221 ggaagagcgt tgccatgtca gatataagcc agctaaatca ttggcatgac catcatcagt
    47281 catctgataa atagtctgaa ttaaatcatc acgattattt attccccagg atgatgagag
    47341 atcgaattct tttatcccgg tatacatttt tggcgagtca tgcgcgggtt tgcaactctg
    47401 attaagtatt tccatgggcg cagaaagcgc aaaaagaaga tattgttctt ccttattcat
    47461 tttttaataa tcccttaagt gcattaactg actacttttt tattattgat gagtataaac
    47521 ttgacgcttt caaaataaaa agaaaatcga agcattcaca catgaataaa aaattaatgt
    47581 atatattcgc aatttttata gttgcagcaa ttacctgtat tagccaaccc aagaaaacga
    47641 tgttgcgtga taaagccatg gtgaattatg cctttgatta tttaagatca ccaggcagtc
    47701 taccgttaac cacggcagcc acggagcttt ccgcgattca tggtcactca acatcgcaat
    47761 atcgccttgg cgaattttat cttcatggta gcgacggtaa accactggat tatacacagg
    47821 cgagatactg gtatgagcaa tcagcagaac aggaaaatcc gcgcgcacaa agcaaactgg
    47881 ggtggatcta cctgaaaggc ctgggagtca aacccgatac ccgtaaagca attctctggt
    47941 ataaggaagc agctgaacaa gggtatgctc atgctcaata tactttgggt ttgatctaca
    48001 gaaatggcac tggtattaat gttaaccatt atgaatctca aaaatggtta aaactggccg
    48061 ccaaacaaca ttacaaaaat gcggaaagat tacttgccgg acttcccgca cattaacgtc
    48121 gactgtcacc cgaagagatg gagtattttc cctatcaaac cggcaaagat aaaaatgtag
    48181 aaaataattt tgatgacgcc cagacagccc agcccgccat catcaacggg cattggctgc
    48241 ggttgcggct ccggctcgac ggtttcctga ccggtaaaat tcgccgcctg aattaataat
    48301 cgacgaataa ttaatgcttc cagtggacaa cgatcttctg catcaaggcg gctcacaatc
    48361 ggcattaatg attcttctgt cggttggctg gcctgcacta attgtttggc aactaaatat
    48421 aatggccata agggatgttc tgtatcacgc gtgagtgaga tccgcggctt acgtcctttg
    48481 tgcaaaacca tctcgaccag tgaacgcccc agcgcagaga gtttttcatc gctgagtatt
    48541 tcgtataaat gaaaacgcgt acgggcgctg tcgccgggga cataaagtag cggttttccc
    48601 tgatcgttaa ccagatcacg cgcttcaatc atcatcacca ctaatgggta ataatagacc
    48661 cgatgctgac ggcaccattg caacattaaa gcatccgcct gggtggaggg atactcgcgc
    48721 cagtagtaac aaagttctgc cagcaggctt tcgccttctc cacaatagac gcgttgtgcg
    48781 tattcataat aacgcgcatc ttcattatca ggttcagctt cccgccacga ccgggcgtaa
    48841 tccaggagtt cggggattcc caggcggctt gcgctatacc agcggagtaa atttctgtgc
    48901 agttttgcat catcggggat aatcactgcg cgatgttgcg ctaaatacaa tgtcagccat
    48961 tctggatgcg atgaccaaat ccgagcgaac atttggtaaa aatcgatggt gtcattttgc
    49021 acggcaactg gcagatgcag aatggtctgg aagttaaaaa cattcccctt cgcaatggca
    49081 tataaaaagg attccagttc ttcttcgtct atttccccga cgttttcctc ctgcaaccac
    49141 cgcaatcttt ctgctaataa caccacgcac tcgaaggata aatgggcagt gttcatggcg
    49201 attgtgcaca gcgaccagcg taattcatca atctcttcca tcgagcaata atttaattgc
    49261 tgaatgaatc gctgccaggc ggagggcaga aaacgttcgc tatcagaggc aagtaacgcg
    49321 cgaaaggcga gcagaatttc atgttctgct acctcatatt cttctggttg ccaaacagat
    49381 ttagcaggcg actgcgcaat ccgtagcgcc tcctcatacg cctgacgaag ttgtttaaaa
    49441 ccctgcggat cggtttctgg atgaaaggac ggtaacagcg ccagataagc gcggcggata
    49501 atatcgacgt cagtcgtttc ctctatatcg aggatcttcc agcaattttt cataagtcgc
    49561 tcatatcctt aagcgtttcc gggcatgtgg ggtagtcagc cagtggttgc caggacaagg
    49621 tagggtaata cgcatcatca ctggcaaata attgataaaa catatcattg ccggaatcgt
    49681 cgtattcttt acgcgctaag aattcgcgta ataaaggcag atgatcatct tcgggagact
    49741 gccaatattg ccgaccaaag gtataaccgg caaagtattg ccgccagtta ctataataac
    49801 gtagagcccg tagatgaata cgcgactgga tccatagact ttcttcttca ctcaaccagt
    49861 tattaagtac gcccattcga cttaaaaaac ccatccggac ataatcccat gctttaattc
    49921 ctccctcacc acagcattcg gctgtgctgg cgacaaattg cgcataagcc tgaccttgct
    49981 catttaattc agcaagatag tcacgccatt cacatggaga ataacgaaac cagcggtgat
    50041 aaaaccctgc caaccgagcg gcatgaccgt tctcgatcat tcggtgaatt atgcgcagca
    50101 ggtcatcacg gtcatcaatt ccccatgagg aagaaaggtc aaaaaagcat gtacctaaat
    50161 acattttggg ggatgaatgc gaaggtaaac agcattcatt gcgtatttcc atcggagtgg
    50221 aaagtgcaaa caatattttt tgcgacccca tgtccatata atttatcctt aaacattcat
    50281 ttaatgaatg taaactacat ttcgaggctc agttatttat acttttctga cgcaacataa
    50341 tacgcataaa ataaggctta cgcaaaaatg gaacacataa aaatataata tattttatct
    50401 acaaccataa ggaaatgata atgattttta cgtcaagttg ctgcgataat ttatcaatag
    50461 atgagattat cgaacgtgct gaaaaaggcg attgcgaggc tcaatatatt gttgggtttt
    50521 attataatcg cgatagcgca attgactctc cggacgacga aaaggccttt tactggctga
    50581 agctggccgc tgagcaaggt cattgtgaag cacagtattc cttagggcaa aagtataccg
    50641 aggataaaag ccgtcataaa gataatgagc aagccatctt ctggctgaaa aaagctgccc
    50701 tacaaggcca tactttcgct tccaacgccc ttggctggat actggatcgt ggagaagacc
    50761 ccaactataa agaagcggtt acctggtatc agatagccgc ggagagcgga atgtcttatg
    50821 cgcaaaataa tcttggatgg atgtacagaa atggcaacgg agtcgcacaa gactatacgc
    50881 tggcattttt ctggtacaaa caagctgcat tacaaggcca tagttacgca caagacaatc
    50941 tggccgatct ttataaagac ggagaaggcg ttgctcaaaa caagacactc gccgcattct
    51001 ggtatttgaa aagcgcacag cagggtaatc ggcacgccca gtttcaaatt gcatgggatt
    51061 ataacgctgg cgaaggggtg gaccaggact ataagcaagc gatgtactgg tatctgaagg
    51121 ctgccgctca ggaaagcgtc ggcgcttacg tcaacatcgg ttatatgtat aaacacggac
    51181 aaggcgttga gaaagattat caggctgcct ttgaatggtt tacgaaagcc gctgaatgca
    51241 atgacgccac tgcctggtat aacctggcca ttatgtatca ctacggagaa ggaagacctg
    51301 tcgatctccg acaggctctc gacctgtatc gtaaagttca gtcatccgga accagagatg
    51361 tcagtcagga aattcgtgag actgaagctt tactgtagaa ataaaaacaa gggccgggga
    51421 atttcattct gcccatattc tctttattgc tgtgcgaaat cagactatta cggctcaaac
    51481 gggcgtctct ggaactggtc ataaccacat ttcgggcata gcgtcagcac ttccggtgtg
    51541 tagatcggga gatggaagtg acatttctcg cagaccagat ttcccagccc gaccacttct
    51601 ccgctgtgat aaaccccatg atgattgagg tcctggaaaa tttcgcgcca ttcaagctgc
    51661 gttttatcgg tgatgtctgc cagctcctgc cacaagcttt ctttaatcac ccgcataaag
    51721 acgctgtcag attcttcttt caggctctct tcatagctca tggcgaactc ttccaggtca
    51781 cgtctgacag ctcgcgtcag ctcatcgatc tcggttcgcg ttaactcccc tgtttttatt
    51841 acgcgctcgc gcgcctgttc caccagtgcg tcgatatcac gttcgccatt gcgcaggcgt
    51901 tcgttcagtg acgcgaccag ttcacggtaa tattgagcaa ccttgttcat cgtttcatct
    51961 cccgggtcgt taactgacta taataataga cgttctttgt tccttgcttt gctaatacgc
    52021 ccacaaactc tgtaaattaa tgtacgaata atccgggcat catttcaacg tcgagtgcgc
    52081 gaaaggctgt tttgacgcgg acgtttgggc tatgctatgc ggatctgaaa aaccacctca
    52141 acgctacatt tgtagccgta ttgaaaacag gaccactggc tgccatgcaa gagcaatacc
    52201 gcccggaaga gatagaatcc aaagtacagc ttcattggga tgagaagcgc acatttgaag
    52261 taaccgaaga cgagagcaaa gagaagtatt actgcctgtc tatgcttccc tatccttctg
    52321 gtcgactaca catgggccac gtacgtaact acaccatcgg tgacgtgatc gcccgttacc
    52381 agcgcatgct gggcaaaaac gttctgcagc cgatcggctg ggacgcgttt ggtctgcctg
    52441 cggaaggcgc ggcggtgaaa aacaacaccg ctccggcacc gtggacgtac gacaacatcg
    52501 cgtatatgaa aaaccagctc aaaatgctgg gctttggtta tgactggagc cgcgagctgg
    52561 caacctgtac gccggaatac taccgttggg aacagaaatt cttcaccgag ctgtataaaa
    52621 aaggcctggt atataaaaag acttctgcgg tcaactggtg cccgaacgac cagaccgtac
    52681 tggcgaacga acaagttatc gacggctgct gctggcgctg cgataccaaa gttgaacgta
    52741 aagagatccc gcagtggttt atcaaaatca ctgcttacgc tgacgagctg ctcaacgatc
    52801 tggataaact ggatcactgg ccagacaccg ttaaaaccat gcagcgtaac tggatcggtc
    52861 gttccgaagg cgtggagatc accttcaacg ttaacgacta tgacaacacg ctgaccgttt
    52921 acactacccg cccggacacc tttatgggtt gtacctacct ggcggtagct gcgggtcatc
    52981 cgctggcgca gaaagcggcg gaaaataatc ctgaactggc ggcctttatt gacgaatgcc
    53041 gtaacaccaa agttgccgaa gctgaaatgg cgacgatgga gaaaaaaggc gtcgatactg
    53101 gctttaaagc tgttcaccca ttaacgggcg aagaaattcc cgtttgggca gcaaacttcg
    53161 tattgatgga gtacggcacg ggcgcagtta tggcggttcc gggtcacgac cagcgcgact
    53221 acgagtttgc ctctaaatac ggcctgaaca tcaaaccggt tatcctggcg gctgacggct
    53281 ctgagccaga tctttctcag caagccctga ctgaaaaagg cgtgctgttc aactctggcg
    53341 agttcaacgg tcttgaccat gaagcggcct tcaacgccat cgccgataaa ctgactgcga
    53401 tgggcgttgg cgagcgtaaa gtgaactacc gcctgcgcga ctggggtgtt tctcgccagc
    53461 gttactgggg cgcgccgatt ccgatggtga cgctggaaga cggtaccgta atgccaaccc
    53521 cggacgacca gctgccggtg atcctgccgg aagatgtggt gatggacggc attaccagcc
    53581 cgattaaagc agatccggag tgggcaaaaa ctaccgttaa cggtatgcca gcgctgcgtg
    53641 aaaccgacac tttcgacacc tttatggagt cctcctggta ctatgcgcgc tacacttgcc
    53701 cagagtacaa agaaggtatg ctggattccg aagcggctaa ctactggctg ccggtggata
    53761 tctacattgg tggtatcgaa cacgccatta tgcacctgct ctacttccgc ttcttccaca
    53821 aactgatgcg tgatgcaggc atggtgaact ctgacgaacc agcgaaacag ttgctgtgtc
    53881 agggtatggt gctggcagat gccttctact atgttggcga aaacggcgaa cgtaactggg
    53941 tttccccggt tgatgctatc gttgaacgtg acgagaaagg ccgtatcgtg aaagcgaaag
    54001 atgcggcagg ccatgaactg gtttataccg gcatgagcaa aatgtccaag tcgaagaaca
    54061 acggtatcga cccgcaggtg atggttgaac gttacggcgc ggacaccgtt cgtctgttta
    54121 tgatgtttgc ttctccggct gatatgactc tcgaatggca ggaatccggt gtggaagggg
    54181 ctaaccgctt cctgaaacgt gtctggaaac tggtgtacga gcacacagca aaaggtgatg
    54241 ttgcggcact gaacgttgat gcgctgactg aagatcagaa agcgctgcgt cgcgatgtgc
    54301 ataaaactat cgctaaagtg accgatgata tcggccgtcg tcagaccttc aacaccgcaa
    54361 ttgcggcgat tatggagctg atgaacaaac tggcgaaagc accgaccgat ggcgagcagg
    54421 atcgcgctct gatgcaggaa gcactgcagg ccgttgtccg tatgctcaac ccgttcaccc
    54481 cgcacatctg cttcacgctg tggcaggaac tcaaaggcga aggcgatatc gacaacgcgc
    54541 cgtggccggt tgctgacgaa aaagcgatgg tggaagactc cacgctggtc gtggtgcagg
    54601 ttaacggtaa agtccgtgcc aaaatcaccg tcccggtgga cgcaacggaa gaacaggttc
    54661 gcgaacgtgc tggccaggaa catctggtag caaaatatct tgatggcgtt actgtacgta
    54721 aagtgattta cgtaccaggt aaactcctca atctggtcgt tggctaagcg cgggaggaag
    54781 cgtgcgatat ctggcaacac tgttgttatc tctggcggtg ttaatcaccg ccgggtgtgg
    54841 ctggcatctg cgtgacacca cgcaggttcc ttccactatg aaggtcatga tcctggactc
    54901 aggcgatccg aacgggccat taagccgtgc ggtgcgtaac cagttacgtc tgaatggtgt
    54961 cgagttgctt gataaagaaa ccacgcgtaa ggacgttcca tccctgcgtt tgggtaaagt
    55021 gagcatcgcg aaagataccg catcggtatt ccgtaacggt caaacagcag agtatcagat
    55081 gatcatgacg gtgaatgcga ccgtgttgat ccccggccgt gatatctacc cgattagcgc
    55141 caaagtcttc cgttcattct tcgataaccc gcaaatggcg ttagcgaaag ataacgaaca
    55201 agacatgatc gtaaaagaga tgtacgaccg tgctgccgaa cagctgattc gtaagctgcc
    55261 aagcatccgt gctgcggata ttcgttccga cgaagaacag acgtcgacca caacggatac
    55321 tccggcaacg cctgcacgcg tctccaccat gctgggtaac tgatgattcg gttgtacccg
    55381 gaacaactcc gcgcgcagct caatgaaggg ctgcgcgcgg cgtatctttt acttggtaac
    55441 gatcctctgt tattgcagga aagccaggac gctgttcgcc aggtagctgc ggcacaagga
    55501 ttcgaagaac accacacttt ttccattgat cccaacactg actggaatgc gatcttttcg
    55561 ttatgccagg ctatgagtct gtttgccagt cgacaaacgc tattgctgtt gttaccagaa
    55621 aacggaccga atgcggcgat caatgaacaa cttctcacac tcaccggact tctgcatgac
    55681 gacctgctat tgatcgtccg cggtaataaa ttaagcaaag cgcaagaaaa tgccgcctgg
    55741 tttactgctc ttgcgaatcg cagcgtgcag gtgacctgtc agacaccgga gcaggctcag
    55801 cttccccgct gggttgctgt gcgcgcaaaa cagctcaact tagaactgga tgacgcggcg
    55861 aatcaggtgc tctgctactg ttatgaaggt aacctgctgg cgctggctca ggcactggag
    55921 cgtttatcgc tgctctggcc tgacggcaaa ctgacattac cgcgcgttga acaggcggtg
    55981 aatgatgccg cgcatttcac cccttttcat tgggttgatg ctttgttgat gggaaaaagt
    56041 aagcgcgcgt tgcatattct tcagcaactg cgtctggaag gcagcgagcc ggttattttg
    56101 ttgcgcacat tacaacgtga actgttgtta ctggttaacc tgaaacgcca gtctgcccat
    56161 acgccactgc gtgcgttgtt tgataagcat cgggtatggc agaaccgccg gggcatgatg
    56221 ggcgaggcgt taaatcgctt aagtcagccg cagttacgtc aggccgtgca actcctgaca
    56281 cgaacggaac tcaccctcaa acaagattac ggtcagtcag tgtgggcaga gctggaaggg
    56341 ttatctcttc tgttgtgcca taaacccctg gcggacgtat ttatcgacgg ttgatatgaa
    56401 atctttacag gctctgtttg gcggcacctt tgatccggtg cactatggtc atctaaaacc
    56461 cgtggaaacg ctggcgaatt tgattggtct gacgcgggtc acaatcatcc ctaataatgt
    56521 tcctccgcat cgtccccagc cggaagcgaa cagcgtgcag cgtaaacaca tgcttgaact
    56581 ggcgattgcc gacaagccat tatttactct tgatgaacgc gagctaaagc gcaatgcccc
    56641 ctcttacact gcgcaaacac tgaaagagtg gcggcaggaa caaggaccgg acgtgccgct
    56701 ggcgtttatt attggtcagg attcattgct gacctttccg acctggtacg aatacgaaac
    56761 gatactcgac aatgcacatt tgatcgtctg tcggcgtcca ggttacccac ttgaaatggc
    56821 gcaaccgcaa taccagcaat ggctggaaga tcatttgaca cataacccgg aagatcttca
    56881 ccttcagcct gccggtaaaa tttatctggc tgaaacgccg tggtttaaca tctcggcgac
    56941 catcatccgc gaacgtttgc aaaacggtga atcgtgtgag gatttattgc cggaaccggt
    57001 attgacttac attaaccaac aaggcttgta tcgctgatac ctgtcgttac ggagggcatg
    57061 atgcgactgt ggttaattcg tcatggcgaa acacaagcga atgtcgatgg tctgtacagc
    57121 ggtcatgcgc ccacacccct gaccgcacgc ggtattgagc aagcgcaaaa tctgcataca
    57181 ctgctagatg atgtttcctt tgacttggtt ttatgcagtg aactggaacg ggcacagcat
    57241 accgcgcgac tggttctcag tgaccgccag ctccccgttc atatcatccc tgaactcaac
    57301 gaaatgtttt ttggcgactg ggagatgcga catcatcgcg acctcatgca agaagatgcc
    57361 gaaaactata gcgcgtggtg caatgactgg cagcatgcta tccccacgaa cggtgaagga
    57421 tttcaggcat tttcgcaacg tgtggaacgc tttatcgcaa ggcttagtga atatcagcac
    57481 tatcagaata ttttaatcgt cagccatcag ggcgtactga gtctgttaat tgctcgttta
    57541 attggcatgc ctgccgagtc tatgtggcat tttcgcgttg accaggggtg ttggagcgcc
    57601 attgatatca accaaaaatt cgcgacgcta cgcgtcctca atagccgtgc catcggggtc
    57661 gagaacgcat gacttttctg tttttttacg ggtaagccgc aacgaccatt gacagccccg
    57721 ggcaggctga tattctccgc agccagactt tttccgccag acacgacttt gtagaaattg
    57781 ttttacaaaa atggcgatgc aatctgcggc gcggggtggg atgatagccc actttcgaaa
    57841 gccgattcgg cgacaattgt cccgaaatcg cctctggttc aggtatactg acagaccatt
    57901 tttatctatt tgattcaccc agggggaaaa cttgcagggt aaagcactcc aggattttgt
    57961 tatcgacaaa attgatgacc tcaaaggtca ggacatcatc gccttagacg ttcaaggcaa
    58021 atccagcatc accgactgca tgatcatctg tacgggtacg tccagccgtc atgttatgtc
    58081 cattgctgac cacgttgtgc aggagtctcg cgcagcgggc ctgttaccgc tcggcgtaga
    58141 aggtgaaaac agcgccgact ggattgtcgt ggatttgggc gatgtgattg tccatgtcat
    58201 gcaggaagag agccgtcgcc tgtatgaact ggaaaaactc tggagttaat gcgtgaagct
    58261 gcaacttgtc gccgtgggaa cgaaaatgcc ggactgggta caaaccggtt ttaccgagta
    58321 cctgcgtcgt tttccgaaag atatgccctt cgagctgatt gaaattccgg ccggcaaacg
    58381 cggcaagaat gcggacatca agcgcatact cgacaaagag ggtgagcaga tgttggcggc
    58441 cgcaggcaaa aaccgcattg tcaccctcga tattccaggc aagccctggg atacgccgca
    58501 gttagccgct gagctggaac gctggaagct ggatggtcgc gacgtaagtc tactgattgg
    58561 cgggcctgaa gggttgtcgc ctgcctgtaa agcggcggct gagcagagct ggtcgctgtc
    58621 ggcgcttacc ctcccccatc cgctggttcg cgtgctggtc gcagagagtc tgtaccgggc
    58681 gtggagcatc accaccaacc atccttatca ccgtgagtga taagggagct ttgagtagaa
    58741 aacgcagcgg atgaaactac agaactcttt tcgcgactat acggcagagt ccgcgctgtt
    58801 tgtgcgccgg gcgctggtcg cctttttggg gattttgctg ctgaccggcg tgcttatcgc
    58861 caacctgtat aatctgcaaa ttgttcgctt taccgactac cagacccgct ctaatgaaaa
    58921 ccgcattaag ctggtgccta tcgcgcccag ccgcggcatt atctacgacc gtaacggtat
    58981 ccctctggcc ctcaaccgca ctatctacca gatagaaatg atgccggaga aagtcgataa
    59041 cgtgcagcaa acgctggacg ctttgcgcag cgtggtagat ctgaccgatg acgatattgc
    59101 tgcattccga aaagagcgcg cacgttcaca ccgtttcacc tctattccgg tgaaaactaa
    59161 cctaaccgaa gtacaagtag ctcgctttgc cgtcaatcag taccgttttc cgggtgtcga
    59221 agttaaaggc tataaacgtc gttactatcc ttacggttcg gcgttgaccc acgtcatcgg
    59281 ctatgtgtcg aaaatcaacg ataaagacgt cgaacgcctg aataatgacg gcaaactggc
    59341 caactatgcg gcaacgcatg atattggtaa gctgggcatt gagcgttact atgaagacgt
    59401 gctgcacggt cagaccggtt atgaagaggt tgaagttaac aaccgtgggc gtgttatccg
    59461 ccagttaaaa gaagtaccac cgcaagccgg acacgatatt tacctgacgc tggatctcaa
    59521 actccagcaa tatattgaaa cgctgctggc gggtagccgc gcagctgtgg ttgttaccga
    59581 accgcgtaca ggtggggtgc tggcgctggt ttccacgcct agttatgacc caaacctgtt
    59641 tgttgacggt atctccagca aagattattc cgccttgctg aacgacccga atacaccgct
    59701 ggtgaaccgc gccacacagg gggtttatcc tcccgcgtct acagttaaac cctatgtggc
    59761 ggtttcggca ttaagcgccg gggtgatcac gcgcaatacg acgctgtttg acccaggctg
    59821 gtggcaacta ccagggtcgg aaaaacgtta tcgtgactgg aaaaaatggg gccacgggcg
    59881 tctgaatgtc acaagatcgc tggaagaatc tgcggatacc ttcttctatc aggtggccta
    59941 cgatatgggg atcgatcgcc tctccgaatg gatgggtaaa ttcggttatg gtcattacac
    60001 cggtatcgac ctggcggaag aacgttccgg caacatgcct acccgcgaat ggaaacagaa
    60061 acgctttaaa aaaccgtggt atcagggtga caccattccg gttggtatcg gccagggtta
    60121 ctggacagcg accccaatcc agatgagtaa ggcactgatg atcctgatta atgacggtat
    60181 cgtgaaggtt ccgcatttgc tgatgagcac cgccgaagac ggcaaacagg tgccatgggt
    60241 acaaccgcat gaaccgcccg tcggcgatat tcattccggt tactgggagc tggcgaaaga
    60301 cggtatgtac ggtgttgcta accgccctaa tggtacggcg aataaatact ttgccagcgc
    60361 accgtacaaa attgcggcga aatctggtac cgctcaggtc ttcggtctga aagcgaacga
    60421 aacctataat gcgcacaaaa ttgccgagcg tttacgtgac cacaaactga tgaccgcctt
    60481 tgcgccatac aacaatccgc aagtggctgt cgccatgatt ctggagaacg gcggcgcggg
    60541 tccggcggtt ggtacgctga tgcgccagat cctcgaccac attatgctgg gtgataacaa
    60601 caccgatctg cctgcggaaa atccagcggt tgccgcagcg gaggaccatt aatcatgacg
    60661 gataatccga ataaaaaaac attctgggat aaagtccatc tcgatcccac aatgctgctg
    60721 attttactgg cattgctggt ttacagcgcc ctggttatct ggagcgccag cggtcaggat
    60781 attggcatga tggagcgtaa aatcggccaa atcgcgatgg gtctggtcat catggtggtg
    60841 atggcgcaaa ttcctccacg cgtttatgaa ggctgggccc cctatctcta tatcatctgt
    60901 attattttgc tggtggcggt agatgctttc ggagccatct ctaaaggcgc tcaacgctgg
    60961 ctggacctcg gtattgttcg ttttcagccg tcggaaattg ccaaaatagc cgtaccactg
    61021 atggttgcgc gctttatcaa ccgcgacgtt tgcccgccat cgttgaagaa tactggcatc
    61081 gcgctggtgc tgatatttat gcccacgctg ctggtggctg cacagcctga cctgggaaca
    61141 tcaatcctcg ttgcgctttc cggtctgttt gtactgttcc tctctggcct tagctggcgt
    61201 ctgattggcg tcgcagtagt gctggtagcg gcgttcattc cgattctgtg gttcttcctg
    61261 atgcatgatt accagcgcca gcgcgtaatg atgctcctgg acccggaatc agacccactc
    61321 ggcgcgggct atcacattat tcagtctaaa attgctattg gctccggcgg attacgcggc
    61381 aaaggctggc tgcacggcac tcagtcacag cttgaatttc tccccgaacg ccatactgac
    61441 tttatcttcg cggtactggc ggaagagctg ggattagtgg gcattctgat tctgctcgct
    61501 ctctacattc tgctgatcat gcgcgggctg tggatagccg ccagagcgca aaccaccttt
    61561 ggtcgcgtca tggctggtgg cttaatgctg atattattcg tttatgtctt cgtaaatatt
    61621 ggtatggtaa gcggtattct gccggttgta ggggttccgc tcccactggt cagttatgga
    61681 ggatcggcgc taattgtgct gatggctggg ttcgggattg taatgtcaat ccacacccac
    61741 aggaaaatgt tgtcgaaaag cgtgtaagag gtgcgcaatg cgtaagcagt ggctcgggat
    61801 ctgcatcgcg gcaggaatgc tcgcggcatg tacaagcgat gatggtcagc aacagacggt
    61861 aagtgtaccg cagcctgcgg tatgtaacgg ccccatagtt gaaattagcg gggcggaccc
    61921 gcgtttcgaa ccactgaacg cgacggcaaa tcaggattac cagcgcgacg gtaaaagcta
    61981 caaaatcgtg caggatccgt ctcgatttag ccaggcggga ctggcggcaa tctatgatgc
    62041 cgaaccaggc agtaacctga cggcctctgg cgaagctttc gatccgacaa agctgacggc
    62101 ggcccatcca acgcttccga tccccagcta cgccagaatc actaacctgg ctaacgggcg
    62161 aatgatcgtg gtgcgcatta atgatcgcgg cccttacggc aacgaccgcg ttatttcgct
    62221 ttctcgcgcg gcagctgacc gtcttaacac gtcaaacaac accaaagttc gtatcgatcc
    62281 gattattgtc gcccaggatg gttcgctttc tggtcctggt atggcttgta ccacagtcgc
    62341 caaacagact tacgccctgc ctgcacctcc cgatttaagc ggtggcgcgg gaacaagttc
    62401 agtgtctggc ccgcagggtg acattcttcc ggtcagtaat tcgacgctaa aaagcgaaga
    62461 tccgaccggc gcgccggtaa ccagtagcgg tttcctcggc gctccaacga ccttagcgcc
    62521 tggtgtactg gaaggcagcg aaccgacgcc tgcgccacag cccgttgtta cagcttcgtc
    62581 gacaacgcct gcaacctcgc ctgcaatggt gacaccgcaa gccgcctcgc aaagcgccag
    62641 cggcaacttt atggtgcaag tcggggccgt aagcgatcag gctcgtgcgc aacagtacca
    62701 acagcaactg ggacagaagt tcggcgtccc cggtcgcgta actcaaaatg gcgcggtctg
    62761 gcggatccag cttggcccat tcgccagcaa agccgaagcc agtaccttgc agcaacgttt
    62821 gcaaaccgaa gcccaattac agtcatttat tactaccgcg cagtagcgta aagcaggcat
    62881 ctgatgtgtc aatttctgta aatgacgtta acaaagtcac gcggaaagtc agatgcctgc
    62941 cggtagtgca tttgctatag tagggcactt ttttaattcc atcacggatg tcgtagttca
    63001 gaccatgaat accatttttt ccgctcgtat catgaagcgc ctggcgctca ccacggctct
    63061 ttgcacagcc tttatctctg ctgcacatgc cgatgacctg aatatcaaaa ctatgatccc
    63121 gggtgtaccg cagatcgatg cggagtccta catcctgatt gactataact ccggcaaagt
    63181 gctcgccgaa cagaacgcag atgtccgccg cgatcctgcc agcctgacca aaatgatgac
    63241 cagttacgtt atcggccagg caatgaaagc cggtaaattt aaagaaactg atttagtcac
    63301 tatcggcaac gacgcatggg ccaccggtaa cccggtgttt aaaggttctt cgctgatgtt
    63361 cctcaaaccg ggcatgcagg ttccggtttc tcagctgatc cgcggtatta acctgcaatc
    63421 gggtaacgat gcttgtgtcg ccatggccga ttttgccgct ggtagccagg acgcttttgt
    63481 tggcttgatg aacagctacg ttaacgcact gggcctgaaa aatacccact tccagacggt
    63541 acatggtctg gatgctgatg gtcagtacag ctccgcgcga gatatggcgc tgatcggcca
    63601 ggcattgatc cgtgacgtac cgaatgaata ctcgatctat aaagaaaaag aatttacgtt
    63661 taacggtatt cgccagctga accgtaacgg cctgttatgg gataacagcc tgaatgtcga
    63721 cggcatcaaa accggacaca ctgacaaagc aggttacaat cttgttgctt ctgcgactga
    63781 aggccagatg cgcttgattt ctgcggtgat gggtggacgt acttttaaag gccgtgaagc
    63841 cgaaagtaaa aaactgctaa cctggggctt ccgtttcttc gaaaccgtta acccactgaa
    63901 agtaggtaaa gagttcgcct ctgaaccggt ttggtttagt gattctgatc gcgcttcgtt
    63961 aggggttgat aaagacgtgt acctgaccat tccgcgtggt cgcatgaaag atctgaaagc
    64021 cagctatgtg ctgaacagca gtgaattgca tgcgccgctg caaaagaatc aggtcgtcgg
    64081 aactatcaac ttccagcttg atggcaaaac gatcgagcaa cgcccgctgg ttgtgttgca
    64141 agaaatcccg gaaggtaact tcttcggcaa aatcattgat tacattaaat taatgttcca
    64201 tcactggttt ggttaaaaat tgaacacttg aaagtgtaat ttccgtcccc atatactaag
    64261 catcagtaaa aaaactcccg ccttctggcg ggagttgcta tttaattacg ttacgccgga
    64321 gctgacatga aaaccaaact taacgaactg cttgaattcc ctactccttt tacttacaaa
    64381 gttatggggc aggcgttacc tgagctggtt gatcaggtgg ttgaagtggt acagcgccat
    64441 gcgccaggtg actacacccc aacggtaaaa ccaagcagca aaggcaacta ccactcggta
    64501 tctatcacta tcaacgccac tcatatcgag caggttgaaa cactgtatga agaactgggc
    64561 aaaatcgata ttgtccgcat ggttctgtaa ctcgcttctc cgttacccgg ctcctggtcg
    64621 ggtaactccc ctctcctcgc tgtgatatac ttttccccca cttttactca ttctccacgg
    64681 agatgccgtt ttgtatcagg ataaaattct tgtccgccag ctcggtcttc agccttacga
    64741 gccaatctcc cgggctatgc atgaattcac cgatacccgc gataatagta cccttgatga
    64801 aatctggctg gtcgagcact atccggtatt cacccaaggt caggcaggaa aagcggagca
    64861 cattttaatg ccgggtgata ttccggtgat ccagagcgat cgcggtgggc aggtgactta
    64921 tcacgggccg ggacaacagg tgatgtatgt gttgcttaac ctgaaacgcc gtaaactcgg
    64981 tgtgcgtgaa ctggtgacct tgcttgagca aacagtggtg aataccctgg ctgaactggg
    65041 tatagaagcg catcctcggg ctgacgcgcc tggtgtctat gtcggggaaa agaaaatttg
    65101 ctcactgggt ttacgaattc gacgcggttg ttcattccac ggtctggcat taaacgtcaa
    65161 tatggatctt tcaccatttt tacgtattaa tccttgtggg tatgccggaa tggaaatggc
    65221 taaaatatca caatggaaac ccgaagcgac gactaataat attgctccac gtttactgga
    65281 aaatatttta gcgctactaa acaatccgga cttcgaatat attaccgctt aattccatac
    65341 atcaatggcc caatttacac tgggtcatta ctcataatac ctgctaatgc tttacatctc
    65401 cttcgcgttt gtatattttc tttatcactt gcagaaatgc ggtgccacgc cttgctccgt
    65461 aactcacccc attgattaac ctattgaaat agttgattat ggtgagcaaa agcgttccgt
    65521 tccaattaga cataagatta ttgtaatttt ttaaatatta tttttccatg aataaaattg
    65581 gagtaagtgc gtggatagta ataatcaaat tgaaccctgt ttaagcagaa agtcgtcaga
    65641 aggtaaacca caaatattta caaccttacg aaatattgat cttaaccttc tgactatttt
    65701 tgaagctgta tatgtacata aagggatcgt taatgcagcg aaagtgctta atctgacccc
    65761 ctcggcaatc agtcagtcta ttcagaaact gcgcgttata ttccctgacc cattgtttat
    65821 tcgcaaaggc cagggtgtca ctcctaccgc atttgcgatg catctacatg agtatatcag
    65881 tcagggcctt gagtccattc ttggcgcgct ggatatcgaa ggaagctatg ataagcaacg
    65941 aacgataact attgcgacca ctccctcggt cggagccctg gtccttcctg tcatctatcg
    66001 ggcgattaaa actcactatc cgcagctttt actgcgcaac ccgcccatca gcgacgcgga
    66061 aaaccaactc agtcagtttc aaaccgatct catcatcgat aacatgtttt gcaccaatcg
    66121 tacggtgcaa catcatgttc tgttcaccga caatatggtg ttaatttgcc gcaagggaaa
    66181 tccactactc tctttagaag atgacagaga gactatcgac aacgctgcgc atgtacttct
    66241 gttaccggaa gggcaaaatt tcagcggtct gcggcagaga gttcaagaga tgtttccgga
    66301 ccggcaaatc aatttcacca gctacaacat tttgacaatc gctgcactgg ttgccaacag
    66361 tgacatgtta gcgattattc ccagccgttt ttataacctg tttagtcgtt gctggccgct
    66421 ggaaaaactg ccttttccgt ccttaaatga ggagcaaata gatttctcta tccactacaa
    66481 caaattcatc ctgcgtgatc ccatcctgca cggagtaata gatgttatcc gtaatgcatt
    66541 ttgaaaaagt attgttacag ccagatcaca gcgcagaacg ataaaatggc acaatccaac
    66601 aacaatttta cattttcgcg accgctttgg ctgctttcag gtccgtttca atgatatact
    66661 gccagtcgtt aattcaaaaa tagttgataa ttacaacaat ctattgaatt gaaacgcttt
    66721 ccttcgtaat tcgcaactgg aacacgcacg ctatgagtaa acccattgtg atggaacgcg
    66781 gtgttaaata ccgcgatgcc gataagatgg cccttatccc ggttaaaaac gtggcaacag
    66841 agcgcgaagc cctgctgcgc aagccggaat ggatgaaaat caagcttcca gcggactcta
    66901 cacgtatcca gggcatcaaa gccgcaatgc gcaaaaatgg cctgcattct gtctgcgagg
    66961 aagcctcctg ccctaacctg gcggaatgct tcaaccacgg cacagcaacg tttatgatcc
    67021 tcggcgctat ttgtacccgc cgttgtccgt tctgtgatgt tgcccacggt cgcccggtag
    67081 ctcctgatgc caatgaacca gtgaaactgg cgcagaccat tgccgatatg gcgctgcgtt
    67141 atgtggttat cacctccgtt gaccgtgatg acctgcgcga tggcggtgcc caacactttg
    67201 cggattgtat tactgccatt cgggaaaaaa gcccgcaaat caaaattgaa actctggtgc
    67261 cggatttccg cggtcgaatg gatcgtgctc tggatattct gactgcaacg cccccagatg
    67321 tgttcaacca taacctggaa aacgtaccgc gtatttaccg gcaggtacgg cctggcgcag
    67381 attacaactg gtcgctgaag ctgctggaac gctttaaaga agcgcatccg gaaatcccga
    67441 ccaagtctgg tctgatggtg ggtctgggtg aaaccaatga agaaattatt gaggtaatgc
    67501 gcgacctgcg ccgtcatggt gtgacgatgt taacgctggg gcaatatttg cagccaagcc
    67561 gccatcacct gccggttcaa cgttacgtta gcccggatga gttcgacgaa atgaaagccg
    67621 aagcgctggc gatgggcttt acccatgctg catgcggtcc gtttgtccgc tcttcttacc
    67681 acgccgattt gcaggcgaaa ggaatggaag ttaagtaatc ctgtaataat tacttacatc
    67741 tgtctgataa aaaacccgct ttttgaagcg ggttttttgt atcgaacaag atattgaggg
    67801 agcgtcctgc tcgccacgtc actctttatg agagagcttt tcagcctgaa gatcaacgtc
    67861 tgcgcctttt ttcgccgcag catcgtcatc attcatcgcc ttcttgaacc ctttaatggc
    67921 cgctccaagg tctccgccca gcgtacgtaa cttcttagtc ccaaacagca gaacgaccag
    67981 cgccgcaact accagcagtt tggtaatact aatctcaccc atagatacct tcttgacata
    68041 taaccggcaa cagaacgcgc cttaaaaacc agagaatatt aacggtcatt cgacgcgcgc
    68101 acacaatagc aatctgtaac aaggtgaatc aagcgccgag taaaaaaaca tcataataat
    68161 tgcggcggcg caaagcgacg gttgtttaag atgggcagtt gcgcgcgcac ctgacgcaca
    68221 cgttcgggcg tcacttccgc cataatgagt gcaggcattt ctgacgctgc cgcaatggtg
    68281 acgccaaagg gatctataat ccggctttgc ccgatatttt tgttcccgca ctcccccgcc
    68341 gccaccatat aacaggtggt atccagcgca cgagcggcaa gcaacgttga ccagtgatgc
    68401 tctttgagcg gcccacgaac ccaggcggca ggaagtacca ggatttcagc tccctgtaaa
    68461 gcctgtgcca gcgccagctc tggaaagcgt aagtcataac aggtcatcag accgaccttc
    68521 atcccttcca cctccagtaa cggagctatt tcattaccag catcaacatg gcgtgattcc
    68581 tgaatggcaa atgcatcata gagatgcagt ttggcataac gggcgacgat gtttcctgcc
    68641 tgaagtgcca ccagcatatt ccatgcgcgc cccggcgttg aaggaagatg aatcgtcaga
    68701 attgtcgtca tcatgttacg tttactttct cgccgtaaac gtccgaggaa ttcgccttcc
    68761 agcagctgtg ctgatttaac cgatagatct gcatcatgat catcgcgcgc cagcaatgct
    68821 tccggcagga caaacagcga tacgtcgttt tccgccgcct gcgccatcaa cgaggcacaa
    68881 atctcagcgt tcttttccca cacagatgta acagcaaact gtcctgcagc aaccagcatt
    68941 gccacctcct gtcagagtcg taaaattcgc caacgacgat tattcggcgt tacacttatc
    69001 actcatacaa atcaaatagc aggattttgc agtgttacaa cttcttttag cagtttttat
    69061 tggcggtggt acgggaagcg tagcgagatg gctgttaagt atgcgattta acccgctgca
    69121 tcaggcgatt ccgttgggga cgctggcagc aaacctgatt ggggcattca tcataggaat
    69181 gggattcgcc tggttcagca ggatgacgaa cattgatcca gtgtggaaag tattaatcac
    69241 caccggattt tgtggcggtc taacaacctt ctcaacattt tcgacagaag tggtgttttt
    69301 gttacaagag ggccgctttg gctgggcatt actgaacgtt ttcgtcaacc ttctggggtc
    69361 ttttgccatg accgcactgg cattctggct gttttcggcc tcaaccgtac actaaagacc
    69421 aaaaaaaacc cgctgattaa gcgggttttg aattcttgct gacgtatctt acagagcgat
    69481 tacgtttgca gcagaagggc ctttggcacc gttagtgatt tcgaactcta cgcgctgacc
    69541 ttcagcaaga gttttaaaac cattagtctg gattgcagag aagtgtacga acacgtcttt
    69601 gctgccgtct tccggagtaa tgaaaccgaa tcctttggac tcattaaacc acttaacgtt
    69661 acctttaatc ttagacatca aaattacctt tacatgaaaa atagacacaa atgctgtgtc
    69721 ggttatcagt acaccaattg tggtacgctt tgtccagtgc aactttacta aaaagtgata
    69781 aaagtcgcgt tatttttgat gttgcttatt ttagttattg attttaaaga agttactaaa
    69841 acttcatttg tctcaaaact gaaagcgcat ccaggcaaag tacacattgc cattgttgta
    69901 ggtacccgga atgtaggtca tctgaaaagt cactgggcca taacccacgg aggccaatgg
    69961 cagtagaacc gggagaggga tgtaattcca gttatcgcgt gccgttacgc cagcggtgaa
    70021 tcccagacct aaatgaaaat tttcatccgc cagcggtcgc caggtacttt cccatccgta
    70081 tccggcaatc ggttcccatt tgttccacga gtccttaaat gccatggcat acaggccatg
    70141 ccagtttcct ttttcatccc aacgcgacag gccaaaaccg ccaccccacg gtcgctcgtt
    70201 atagcgatcg gttttttctt tgtcgtaagc gaaacgtgca tgccaggtga tggcaggaat
    70261 atataaatca taatgttcag gctgttgcca ggtttgtgca atattttctc taaacgttgt
    70321 catcctctca tctgcgttag caaaaacttt accaacgctg attaactgaa taaaaacaaa
    70381 ggaaaagata gcgacatatt tactcacgtt catttgtgac cataaaacat ttatcaaaaa
    70441 tctactacta gcatagcaaa gctacaatta acataacctt aatagaccca acataaagaa
    70501 taatctgaat agctgctcta tttatttccg cttgttcaag ttaaatattt ccgcgcatcg
    70561 attaaagatg atgcgtcgtt ttctgcgatg ggaatagtca aaaaagaaaa accaagtctt
    70621 ttttgatgac aaaatgcaat caaggaaaaa ttattttatt tttttaacga gatacaacaa
    70681 tcatcttaac gaagtatata atatatatcg tcaacccatg ctcatattag attatttttt
    70741 gagcgcacag ccgcagctaa caccattgca attaacaaat ttgcatcaat ccaccatcaa
    70801 tttacacaca ttattatgtg ataattgcca accgctaaat atgcgttttg ttatctatgt
    70861 ataaaaacag caacttcaat gtcttaatgg cagtttttct tgattttaat cagcattcat
    70921 cgccaattta ttgggcatat tttttcctta agctttagga agtttttatt atttactttg
    70981 ggcctggaga caggaaaaat tatgctgaca ttcattgaac tccttattgg ggttgtggtt
    71041 attgtgggtg tggctcgcta catcattaaa gggtattccg ccactggtgt gttatttgtc
    71101 ggtggcctgt tattgctgat tatcagtgcc attatggggc acaaagtgtt accgtccagc
    71161 caggcttcaa caggctacag cgccacggat atcgttgaat acgttaaaat attactaatg
    71221 agccgcggcg gcgacctcgg catgatgatt atgatgctgt gtggctttgc cgcttacatg
    71281 acccatatcg gcgcgaatga tatggtggtc aagctggcgt caaaaccatt gcagtatatt
    71341 aactcccctt acctgctgat gattgccgcc tattttgttg cttgtctgat gtcactggcc
    71401 gtctcttccg caaccggtct gggggttttg ctgatggcaa ccctgtttcc ggtgatggta
    71461 aacgttggta tcagtcgtgg cgcagcagct gccatttgtg cctccccggc ggcgattatt
    71521 ctcgcaccga cttcagggga tgtggtgctg gcggcgcagg cttccgaaat gtcgctgatt
    71581 gacttcgcct tcaaaacgac gctgcctatc tcaattgctg caattatcgg catggcgatc
    71641 gcccacttct tctggcaacg ttatctggat aaaaaagagc acatctctca tgaaatgtta
    71701 gatgtcagtg aaatcaccac cactgccccc gcgttttatg ccattttgcc gttcacgccg
    71761 atcatcggtg tactgatttt tgacggtaaa tggggtccgc aattacacat catcactatt
    71821 ctggtgattt gtatgctgat tgcctccatt ctggagttcc tccgcagctt taatacccag
    71881 aaagttttct ctggtctgga agtggcttat cgcgggatgg cagatgcgtt tgctaacgtg
    71941 gtgatgctgc tggttgccgc tggggtattc gctcaggggc ttagcaccat cggctttatt
    72001 caaagtctga tttctatcgc tacctcgttt ggttcggcga gtatcatcct gatgctggta
    72061 ttggtgattc tgaccatgct ggcggcagtc acgaccggtt caggcaatgc gccgttttat
    72121 gcgtttgttg agatgatccc gaaactggcg cactcttccg gcattaaccc ggcgtatttg
    72181 actatcccga tgctgcaggc gtcaaacctg ggtcgtaccc tatcacccgt ttctggcgta
    72241 gtcgttgcgg ttgccgggat ggcgaaaatc tcaccatttg aagtcgtaaa acgcacctcg
    72301 gtgccggtgc ttgttgggct agtgattgtt atcgttgcta cagagctgat ggtgccagga
    72361 acggcagcag cggtcacagg caagtaaata gtaatgccgg aggcaagttt cctccggctt
    72421 tatcatgtca cccactgtgg tatatgcgtt gtggtctgcc aactttgccg tgaacaattt
    72481 cagcaataat cagatggcgg ctggcgcaat attcaagata acgcctggca gtggtgcggc
    72541 tgatggtcag tgcctgcgcc accgtttctg ccgtatgttg cacaccaggc tctttaaaca
    72601 gttttcgcac cgcgtttagc gtcaaggggt caatgccggt cggcagttcg tctttgggtt
    72661 caccgcgagc ataggcatta aacatctcat caatttgctt ctggctggcg ctatcaatac
    72721 tttccagcat atgtttacgc tggcggaaac gggttagcgt ttgccccagt gtattgattc
    72781 acttgaagta cgaaaaaacc gggagaacat tggattattc gggatctgat gggattagat
    72841 ttggtggggc ttgcaagcct gtagtacaaa ttttaggcgt taaccaatga aacgcgaaag
    72901 atagtaaaaa attgcttttg tttcattgaa aaaacgaaaa acaaaaacac tataaatcat
    72961 ttcaatcata gcttcaaaaa acgttcaaaa ccgataacaa ccaggctgtc accaaatgac
    73021 tcatatcaca aatcagctca tgccgtttag gtatgttaca tgtgtgatta tgtgaggtga
    73081 agtatgtttt agctggctca tggttgttat acggcttttt tcgcatcttg tggttccaac
    73141 aaaggagcta caggctcttc ctgtttatga acaccataca ttgacaaaat ctcttggcga
    73201 gtttctggag gtaatttatg cagcgccaaa gctgtttgta gtgtttggcg atcaataccc
    73261 agcgttgcca atagggtgtc aacggagatg gaggggcgtt cttgcattaa tccggaaagc
    73321 ccatatttag ccatgtttag ctctatctct cggacttcat tttctcctag tttattgatt
    73381 aacagggaga acattttcaa tgtatcagca ttcatttctt cgatagcaat tttttgcata
    73441 gccggtattg ccgggatata ttcaacccca ggcccgcgac gcccttcttg tgaacgcttc
    73501 ggccatcctt ctttctttgc tcgtttatgc accccctgta ttgtcccagg cattccatcc
    73561 agttcagtaa tttgtttggg tgtcatccac tggtttatct catttgcgct tttcataaac
    73621 taagtttacc agatttatct atataaaaat caatggatta caaaataaac cacattaact
    73681 gatataaacc ggtttggttt atttggtaat gtataaaaca gataatcaag acgaattatt
    73741 tgttttggta accctttatc atacgcaata tggttggtgt gcgaaagatg agtaacgatt
    73801 ggcatcgggc cgacgtgatt gcggccctca aaaaaacagg aacccctctg gcaaaattgt
    73861 ccagacgttc aggttattca accgggacgc ttgcaaatgc attagtcagg ccatggccta
    73921 aaggtgaacg cattattgct gacgccttaa acatgaaacc tgaggaaatt tggcctagta
    73981 gatatctaag ggatcacaat gagagagtgg atatcagcaa ttgaatgcat gggtttatct
    74041 ggtttacctg gttcattaat gggggttcac aaaagagctc agagagaagg atggctcaag
    74101 agaagaagag aaggaattag aggggttgct tacgaatacc atatatcttc tcttcctaac
    74161 attgctcgag ctgaagtttt gctccaacaa ggaaaattta aaaccagtca gggatgtttt
    74221 gaaatcgccc gccccacgct ggaagctcat gattatgatc gtgaggcact atggagcaaa
    74281 tgggataaag ccagcgattc ccagcgcagc ctggctgaaa aatggttgcc ctcaattcag
    74341 gccacagacg aaatgctgaa ccaggggatt tcaacgaaaa cggcttttgc gaccgttgca
    74401 gggcattacc aggtcagcgc atccactttg cgggacaagt attaccaggt acagaagttt
    74461 gcgaagcctg actgggcggc tgcacttgtt gatggacgtg gagcatcccg tcgcaatgtt
    74521 cacaaaagtg aatttgacga ggatgcctgg cagtttctga ttgcagatta tctgcgaccg
    74581 gaaaaacccg ccttccgcaa atgttatgag cgtctggaac tggcagcccg cgagcatggc
    74641 tggagtattc cctcccgtgc cacggccttt cgccggattc agcaactgga cgaggcaatg
    74701 gttgttgcct gtcgtgaagg tgaacatgca ctgatgcatc tgataccggc acagcagcga
    74761 actgtggaac acctggacgc catgcagtgg atcaacggcg acggttatct gcataacgtc
    74821 tttgtacgct ggtttaacgg tgatgtgatc cgtccgaaaa catggttctg gcaggatgtg
    74881 aaaacccgaa aaattctggg ctggcgctgc gatgtgagcg agaacattga ttcaattcgc
    74941 ctctcgttca tggatgttgt gactcgctac agtatcccgg aggattttca catcaccatt
    75001 gataacaccc gtggtgctgc gaataaatgg ctgacgggag gcgcgcccaa tcgctaccgc
    75061 tttaaggtaa aagaggacga tccaaaagga ctgtttttac tgatgggtgc gaaaatgcac
    75121 tggacaagcg ttgttgccgg taaaggctgg ggccaggcaa aacctgttga acgtgctttc
    75181 ggtgttggtg ggcttgagga atacgttgat aagcatccgg cactggctgg cgcatatacg
    75241 gggccaaatc cgcaggcaaa acctgataac tatggcgacc acgctgttga tgcagagctg
    75301 tttctgaaaa cctttgccga aggtgtggcg atgttcaatg ccagaacagg ccgtgaaaca
    75361 gaaatgtgcg ggggcaaact ctcgtttgat gatgttttcg agcgtgaata cgccagaacg
    75421 attgtgcgta agccaaccga agaacaaaaa cggatgctgt tactgcctgc cgaggcggtg
    75481 aacgtttcac gcaaaggcga gtttacgctt aaagttggcg gctcccttaa aggcgcgaaa
    75541 aacgtttatt acaacatggc attaatgaat gccggagtga aaaaagttgt ggtcagattt
    75601 gatccacagc agttacacag cacggtttat tgag
//
LOCUS       DAJTSA010000004        73321 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000004 DAJTSA010000000
VERSION     DAJTSA010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 73321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.004"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..592)
                     /locus_tag="OO369_000282"
     CDS             complement(<1..592)
                     /locus_tag="OO369_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR5980683.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLD"
     gene            complement(663..2777)
                     /gene="fusA"
                     /locus_tag="OO369_000283"
     CDS             complement(663..2777)
                     /gene="fusA"
                     /locus_tag="OO369_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR5980684.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2874..3344)
                     /gene="rpsG"
                     /locus_tag="OO369_000284"
     CDS             complement(2874..3344)
                     /gene="rpsG"
                     /locus_tag="OO369_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR5980685.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3441..3815)
                     /gene="rpsL"
                     /locus_tag="OO369_000285"
     CDS             complement(3441..3815)
                     /gene="rpsL"
                     /locus_tag="OO369_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR5980686.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3941..4228)
                     /gene="tusB"
                     /locus_tag="OO369_000286"
     CDS             complement(3941..4228)
                     /gene="tusB"
                     /locus_tag="OO369_000286"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR5980687.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4236..4595)
                     /gene="tusC"
                     /locus_tag="OO369_000287"
     CDS             complement(4236..4595)
                     /gene="tusC"
                     /locus_tag="OO369_000287"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR5980688.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4595..4981)
                     /gene="tusD"
                     /locus_tag="OO369_000288"
     CDS             complement(4595..4981)
                     /gene="tusD"
                     /locus_tag="OO369_000288"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR5980689.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4981..5703)
                     /locus_tag="OO369_000289"
     CDS             complement(4981..5703)
                     /locus_tag="OO369_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5980690.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5870..6682)
                     /gene="fkpA"
                     /locus_tag="OO369_000290"
     CDS             complement(5870..6682)
                     /gene="fkpA"
                     /locus_tag="OO369_000290"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5980691.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6903..7121
                     /locus_tag="OO369_000291"
     CDS             6903..7121
                     /locus_tag="OO369_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR5980692.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7170..7760)
                     /gene="slyD"
                     /locus_tag="OO369_000292"
     CDS             complement(7170..7760)
                     /gene="slyD"
                     /locus_tag="OO369_000292"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5980693.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7855..8055)
                     /locus_tag="OO369_000293"
     CDS             complement(7855..8055)
                     /locus_tag="OO369_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR5980694.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(8065..9802)
                     /gene="kefB"
                     /locus_tag="OO369_000294"
                     /pseudo
     CDS             complement(8065..9802)
                     /gene="kefB"
                     /locus_tag="OO369_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9802..10353)
                     /gene="kefG"
                     /locus_tag="OO369_000295"
     CDS             complement(9802..10353)
                     /gene="kefG"
                     /locus_tag="OO369_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR5980695.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10484..12397
                     /locus_tag="OO369_000296"
     CDS             10484..12397
                     /locus_tag="OO369_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5980696.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12397..13419
                     /locus_tag="OO369_000297"
     CDS             12397..13419
                     /locus_tag="OO369_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5980697.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13413..13631
                     /locus_tag="OO369_000298"
     CDS             13413..13631
                     /locus_tag="OO369_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR5980698.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13685..14554
                     /locus_tag="OO369_000299"
     CDS             13685..14554
                     /locus_tag="OO369_000299"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR5980699.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14609..15013)
                     /locus_tag="OO369_000300"
     CDS             complement(14609..15013)
                     /locus_tag="OO369_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR5980700.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15315..15947
                     /gene="crp"
                     /locus_tag="OO369_000301"
     CDS             15315..15947
                     /gene="crp"
                     /locus_tag="OO369_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR5980701.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15998..18088
                     /locus_tag="OO369_000302"
     CDS             15998..18088
                     /locus_tag="OO369_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR5980702.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18155..19375)
                     /gene="argD/dapC"
                     /locus_tag="OO369_000303"
     CDS             complement(18155..19375)
                     /gene="argD/dapC"
                     /locus_tag="OO369_000303"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR5980703.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19461..20024)
                     /gene="pabA"
                     /locus_tag="OO369_000304"
     CDS             complement(19461..20024)
                     /gene="pabA"
                     /locus_tag="OO369_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR5980704.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(20056..20658)
                     /locus_tag="OO369_000305"
     CDS             complement(20056..20658)
                     /locus_tag="OO369_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR5980705.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20648..20815)
                     /locus_tag="OO369_000306"
     CDS             complement(20648..20815)
                     /locus_tag="OO369_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR5980706.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20920..21510)
                     /gene="ppiA"
                     /locus_tag="OO369_000307"
                     /gene_synonym="rotA"
     CDS             complement(20920..21510)
                     /gene="ppiA"
                     /locus_tag="OO369_000307"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR5980707.1"
                     /translation="MFKSTLAAMAAVFALSALSALSALSPAAMAAKGDPHVLLTTSAG
                     NIELELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPP
                     IKNEADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVK
                     GMDVADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21781..22962
                     /gene="tsgA"
                     /locus_tag="OO369_000308"
     CDS             21781..22962
                     /gene="tsgA"
                     /locus_tag="OO369_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR5980708.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23224..25767
                     /gene="nirB"
                     /locus_tag="OO369_000309"
     CDS             23224..25767
                     /gene="nirB"
                     /locus_tag="OO369_000309"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR5980709.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25764..26090
                     /gene="nirD"
                     /locus_tag="OO369_000310"
     CDS             25764..26090
                     /gene="nirD"
                     /locus_tag="OO369_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR5980710.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26216..27022
                     /gene="nirC"
                     /locus_tag="OO369_000311"
     CDS             26216..27022
                     /gene="nirC"
                     /locus_tag="OO369_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR5980711.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            27041..28414
                     /gene="cobA"
                     /locus_tag="OO369_000312"
     CDS             27041..28414
                     /gene="cobA"
                     /locus_tag="OO369_000312"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5980712.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28661..28828
                     /locus_tag="OO369_000313"
     CDS             28661..28828
                     /locus_tag="OO369_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR5980713.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            29123..30460
                     /gene="frlA"
                     /locus_tag="OO369_000314"
                     /pseudo
     CDS             29123..30460
                     /gene="frlA"
                     /locus_tag="OO369_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30481..31503
                     /gene="frlB"
                     /locus_tag="OO369_000315"
     CDS             30481..31503
                     /gene="frlB"
                     /locus_tag="OO369_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR5980714.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31553..32383
                     /gene="frlC"
                     /locus_tag="OO369_000316"
     CDS             31553..32383
                     /gene="frlC"
                     /locus_tag="OO369_000316"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR5980715.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERSRALLPEDER
                     "
     gene            32380..33165
                     /gene="frlD"
                     /locus_tag="OO369_000317"
     CDS             32380..33165
                     /gene="frlD"
                     /locus_tag="OO369_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR5980716.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            33265..33996
                     /gene="phoB"
                     /locus_tag="OO369_000318"
     CDS             33265..33996
                     /gene="phoB"
                     /locus_tag="OO369_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR5980717.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(34156..35241)
                     /locus_tag="OO369_000319"
     CDS             complement(34156..35241)
                     /locus_tag="OO369_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5980718.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(35253..36557)
                     /locus_tag="OO369_000320"
     CDS             complement(35253..36557)
                     /locus_tag="OO369_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR5980719.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36569..36922)
                     /locus_tag="OO369_000321"
     CDS             complement(36569..36922)
                     /locus_tag="OO369_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR5980720.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36933..37811)
                     /locus_tag="OO369_000322"
     CDS             complement(36933..37811)
                     /locus_tag="OO369_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR5980721.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37808..39034)
                     /locus_tag="OO369_000323"
     CDS             complement(37808..39034)
                     /locus_tag="OO369_000323"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5980722.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(39035..40198)
                     /locus_tag="OO369_000324"
     CDS             complement(39035..40198)
                     /locus_tag="OO369_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR5980723.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(40282..40644)
                     /locus_tag="OO369_000325"
     CDS             complement(40282..40644)
                     /locus_tag="OO369_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR5980724.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40661..41566)
                     /locus_tag="OO369_000326"
     CDS             complement(40661..41566)
                     /locus_tag="OO369_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5980725.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41836..42840)
                     /gene="trpS"
                     /locus_tag="OO369_000327"
     CDS             complement(41836..42840)
                     /gene="trpS"
                     /locus_tag="OO369_000327"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR5980726.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42833..43591)
                     /gene="gph"
                     /locus_tag="OO369_000328"
     CDS             complement(42833..43591)
                     /gene="gph"
                     /locus_tag="OO369_000328"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR5980727.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43584..44261)
                     /gene="rpe"
                     /locus_tag="OO369_000329"
     CDS             complement(43584..44261)
                     /gene="rpe"
                     /locus_tag="OO369_000329"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR5980728.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(44279..45115)
                     /gene="dam"
                     /locus_tag="OO369_000330"
     CDS             complement(44279..45115)
                     /gene="dam"
                     /locus_tag="OO369_000330"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5980729.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(45222..46514)
                     /gene="damX"
                     /locus_tag="OO369_000331"
     CDS             complement(45222..46514)
                     /gene="damX"
                     /locus_tag="OO369_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR5980730.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46606..47694)
                     /gene="aroB"
                     /locus_tag="OO369_000332"
     CDS             complement(46606..47694)
                     /gene="aroB"
                     /locus_tag="OO369_000332"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR5980731.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47751..48272)
                     /gene="aroK"
                     /locus_tag="OO369_000333"
     CDS             complement(47751..48272)
                     /gene="aroK"
                     /locus_tag="OO369_000333"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR5980732.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48673..49911)
                     /gene="hofQ"
                     /locus_tag="OO369_000334"
     CDS             complement(48673..49911)
                     /gene="hofQ"
                     /locus_tag="OO369_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR5980733.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49823..50229)
                     /gene="hofP"
                     /locus_tag="OO369_000335"
                     /pseudo
     CDS             complement(49823..50229)
                     /gene="hofP"
                     /locus_tag="OO369_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            complement(50219..50659)
                     /gene="hofO"
                     /locus_tag="OO369_000336"
     CDS             complement(50219..50659)
                     /gene="hofO"
                     /locus_tag="OO369_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR5980734.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50643..51182)
                     /gene="hofN"
                     /locus_tag="OO369_000337"
     CDS             complement(50643..51182)
                     /gene="hofN"
                     /locus_tag="OO369_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR5980735.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(51182..51961)
                     /gene="hofM"
                     /locus_tag="OO369_000338"
     CDS             complement(51182..51961)
                     /gene="hofM"
                     /locus_tag="OO369_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR5980736.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            52081..54633
                     /gene="mrcA"
                     /locus_tag="OO369_000339"
     CDS             52081..54633
                     /gene="mrcA"
                     /locus_tag="OO369_000339"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR5980737.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54798..55358)
                     /gene="nudE"
                     /locus_tag="OO369_000340"
     CDS             complement(54798..55358)
                     /gene="nudE"
                     /locus_tag="OO369_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR5980738.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55678..57813
                     /gene="igaA"
                     /locus_tag="OO369_000341"
     CDS             55678..57813
                     /gene="igaA"
                     /locus_tag="OO369_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR5980739.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57878..58546
                     /gene="yrfG"
                     /locus_tag="OO369_000342"
     CDS             57878..58546
                     /gene="yrfG"
                     /locus_tag="OO369_000342"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR5980740.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58557..58958
                     /gene="hslR"
                     /locus_tag="OO369_000343"
     CDS             58557..58958
                     /gene="hslR"
                     /locus_tag="OO369_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR5980741.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58983..59852
                     /gene="hslO"
                     /locus_tag="OO369_000344"
     CDS             58983..59852
                     /gene="hslO"
                     /locus_tag="OO369_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR5980742.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNTMD
                     IAEIRNNASPADP"
     gene            complement(59996..61747)
                     /locus_tag="OO369_000345"
     CDS             complement(59996..61747)
                     /locus_tag="OO369_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR5980743.1"
                     /translation="MDNVELSPATRWGMIATGLLQGMIATGLLQGLVCYLLIAWLAGK
                     NHSWIVYGVPATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNP
                     WRAEKAIWDFGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLS
                     NGLTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDS
                     IQKLFTLIATGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAI
                     VRDPQKASLPWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAEL
                     VLLVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSH
                     MARYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQH
                     LQEQVSEKVFAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDG
                     QAERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKA
                     WRDLTVDGETLEINLSK"
     gene            62126..63748
                     /gene="pckA"
                     /locus_tag="OO369_000346"
     CDS             62126..63748
                     /gene="pckA"
                     /locus_tag="OO369_000346"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR5980744.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63824..65176)
                     /gene="envZ"
                     /locus_tag="OO369_000347"
     CDS             complement(63824..65176)
                     /gene="envZ"
                     /locus_tag="OO369_000347"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR5980745.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(65173..65892)
                     /gene="ompR"
                     /locus_tag="OO369_000348"
     CDS             complement(65173..65892)
                     /gene="ompR"
                     /locus_tag="OO369_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR5980746.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            66120..66596
                     /gene="greB"
                     /locus_tag="OO369_000349"
     CDS             66120..66596
                     /gene="greB"
                     /locus_tag="OO369_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR5980747.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66693..69014
                     /locus_tag="OO369_000350"
     CDS             66693..69014
                     /locus_tag="OO369_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR5980748.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69450..69677
                     /gene="feoA"
                     /locus_tag="OO369_000351"
     CDS             69450..69677
                     /gene="feoA"
                     /locus_tag="OO369_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR5980749.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69694..72015
                     /gene="feoB"
                     /locus_tag="OO369_000352"
     CDS             69694..72015
                     /gene="feoB"
                     /locus_tag="OO369_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR5980750.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            72015..72251
                     /gene="feoC"
                     /locus_tag="OO369_000353"
     CDS             72015..72251
                     /gene="feoC"
                     /locus_tag="OO369_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR5980751.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72454..>73321
                     /gene="rpnA"
                     /locus_tag="OO369_000354"
     CDS             72454..>73321
                     /gene="rpnA"
                     /locus_tag="OO369_000354"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR5980752.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAA"
BASE COUNT        17663 a        19873 c        18555 g        17230 t
ORIGIN      
        1 aatccaggaa gccagccagt tccaggattt tcgcttccca ctctgcgtcg ccttccagcg
       61 ctttcagagc agaaccacga acgatcggag tgtcgtcgcc cgggaagtcg tactgagaca
      121 gaagttcacg aacttccatt tcaaccagtt ccagcagctc ttcgtcatca accatgtcgc
      181 atttgttcag gaacacgatg atgtacggaa cgcctacctg acgacccagc aggatgtgct
      241 cacgagtctg cggcatcggg ccgtcagtcg cagcaactac caggatcgcg ccgtccatct
      301 gagcagcacc ggtgatcatg tttttaacat agtcggcgtg ccccgggcag tctacgtgtg
      361 cgtagtgacg ggtcggggtg tcatattcaa cgtgagaagt gttgatggtg ataccacgag
      421 ctttttcttc cggcgcgtta tcgatctggt cgaatgcacg agcagcaccg ccgtaggttt
      481 tagccagtac ggtggtgatt gcagcggtca gagtagtttt accgtggtca acgtggccga
      541 tagtaccaac gttaacgtgc ggttttgtac gttcaaattt ttctttagac acggctatat
      601 tccttactat agtgctctcc ccttcaggag agagcacggg actttggtat taacccttag
      661 gcttatttac cacgggcttc aattacggcc tgagcaacgt tactcggcgc ttcatcatac
      721 ttcaggaatt ccatagtgta tgatgcacga cctttggtca gagaacgcag ctgagttgcg
      781 tatccgaaca tttcagacag cggtacttca gcgtggatct taacgccagt aacttcagat
      841 tcctgacctt tgagcatacc acgacgacgg ctcaagtcac cgataacgtc accggtgttc
      901 tcttccggag tttctacttc aaccttcatg atcggctcaa gcagaactgg tttcgctttc
      961 ttaaagcctt ctttaaaggc gatagaagca gccagtttaa acgccagttc agaggagtca
     1021 acgtcatggt aagaaccgaa gtgcagacga atacccatgt ctactaccgg gtagcctgcc
     1081 agcggacctg ctttcagctg ttcctggata cctttatcaa cggccgggat gtattcgcca
     1141 gggattacac cacctttaat gtcgttgatg aactcgtagc ctttcgggtt tgaacccggc
     1201 tccagcgggt acatgtcgat aacaacatga ccatactgac cacgaccacc agactgtttc
     1261 gcgtgtttac cttcaacatc ggtaactttc tggcggatag tttcacggta agcaacctgc
     1321 ggtttaccta cgttcgcttc aacgttgaat tcacgcttca tacggtcaac gatgatgtcg
     1381 aggtgcagtt cgcccatacc cgcgatgatg gtctggttag attcttcgtc agtccataca
     1441 cggaaagacg ggtcttcttt agccagacgg cccagagcca gacccatttt ttcctggtca
     1501 gctttggttt tcggttcaac tgcgatggag attaccggct cagggaattc catacgttcc
     1561 agaatgatcg gcgcatccgg gtcacacagg gtgtcaccag tggttacgtc tttcaggccg
     1621 atagcagcag cgatgtcgcc cgcgcgaact tctttgatct cttcacgttt gttagcgtgc
     1681 atctgaacga tacgaccgaa acgctcacgt gcagctttca cggagttcag tacggtatca
     1741 ccagagttaa ccacaccgga gtaaacacgg aagaaggtca ggttaccaac aaacgggtcg
     1801 gtagcgattt tgaacgccag tgcagagaac ggctcgtcat cacttgcgtg acgttcagcc
     1861 ggagtgtctt taccgtcgtc caggataccg ttgatcgcag gtacgtcaac cggggatggc
     1921 aggtaatcaa ttaccgcatc cagcatcgcc tgaacacctt tgttcttgaa cgcagaacca
     1981 caggttacca ggatgatttc gttgttcaga acgcgctgac gcagagcacc tttgatttct
     2041 gcttcagtca gttcttcacc acccaggtat ttttccatca gctcttcaga agcttcagct
     2101 gcggattcga tcaggttctg gtgccattcg ttagccagtt caaccatgtc tgccgggata
     2161 tcttcgtatt cgaaggttac gccctggtca gcgtcattcc agttgatagc tttcattttc
     2221 accaggtcaa caacaccggt gaaatgttct tcagcaccaa tcgccagctg cagcggaacc
     2281 gggttcgcgc ccagacgggt tttgatctgg ttaacaactt tcaggaagtt cgcacccacg
     2341 cggtccattt tgttaacgaa cgcaatgcgc ggaactttat atttgtttgc ctgacgccat
     2401 acggtttcag actgcggctg aacaccacca actgcgcagt aaaccattac cgcaccatcg
     2461 agaacacgca tggaacgttc tacttcgatt gtgaagtcaa cgtgccccgg ggtgtcgatg
     2521 atgttgatgc gatgcggctc atactgctta gccataccag accagaatgc agtagtcgca
     2581 gcggaagtga tggtaatacc acgttcctgc tcctgctcca tccagtccat ggttgcagcg
     2641 ccgtcatgaa cttcaccgat tttatggttt acaccggtgt agaacagaat acgttcggta
     2701 gtagtggttt taccggcgtc gatgtgcgca ctgataccga tgttacggta gcgtgcgatg
     2761 ggtgttgtac gagccatttg tttcctcgtt tatcttttag gcgttcaatt taagtagccc
     2821 aaagcgggct gcttactgga agcgcccgcc tggtgactaa aactccgaag ggattaccaa
     2881 cggtagtgtg cgaacgcctt gttggcttcg gccatacggt gaacgtcttc acgtttctta
     2941 actgcagtac ctttgttttc tgcagcatca gaaagttcgt tcgccaggcg cagagccatg
     3001 gatttatcac cgcgtttacg agcagcttca acgatccaac gcattgccag agcattacga
     3061 cgaaccggac ggacttcaac tggtacctga taagtagaac caccaacgcg gcgagactta
     3121 acttctacag tcgggcgcac gttttcgaga gctacttcga atgcttccag ttcagattta
     3181 ccagagcgct gagccagggt ctccagcgcg ctgtatacga tagattcagc agtagatttt
     3241 ttaccatcta ccatcaggat atttacaaat ttagccagca gttctgatcc gaacttcgga
     3301 tccggcagaa ttttacgctg accaatgacg cgacgacgtg gcatggaaat actccgttgt
     3361 taattcagga ttgtccaaaa ctctacgagt ttagtttgac atttaagtta aaacgtttgg
     3421 ccttacttaa cggagaacca ttaagcctta ggacgcttca cgccatactt ggaacgagcc
     3481 tgcttacggt ctttaacgcc ggagcagtca agcgcaccac gtacggtgtg gtaacgaaca
     3541 cccgggaggt ctttaacacg accgccacgg atcaggatca cggagtgctc ctgcaggttg
     3601 tgaccttcac caccgatgta ggaagtcact tcgaaaccgt tagtcagacg aacacggcat
     3661 actttacgca gcgcggagtt cggtttttta ggagtggtag tatatacacg agtacatacg
     3721 ccacgttttt gcgggcatgc ttccagcgca ggcacgttgc ttttcgcaac tttgcgagca
     3781 cgtggtttgc gtaccagctg gttaactgtt gccattaaat agctcctggt tttagctttt
     3841 gcttcgtaaa cacgtaataa aacgtcctca cacaatatga ggacgccgaa ttttagggcg
     3901 atgccgaaaa ggtgtcaaga aatatacaac gatcccgcca tcaccaggcc atctggctgg
     3961 agtgcttaac cgtaagtctg acgaaatcag tatagtcaat gggaatgatg tcgttcaaaa
     4021 tttgaccagt caaaccgcgg gcaataaggt cttcgttcag ggcatagacc ttaatggggg
     4081 cattacgcag actttcaagg tagcggttac cgtcaactgc ggcagttacg ccatcttgca
     4141 ataatagcag ttcgtctcct tcactgagca gacgcagcag cgcagcaaaa tccgtcagcc
     4201 agggtgaacg atgtaacgtg tgcagcataa acgcctcaaa acctcaaaat aacatcgtag
     4261 ttggcgagtt cgcggcgtaa ggcatctgct tcgagcggcg tggcttcgac aacaaagggt
     4321 gtctgcggat ctaacccgcg ttcgcgcagt gaagccgcac aaacccagca ctgttcaatg
     4381 tcgtacagac tcaacaattt aaaagtggca atgtaatcac gcgccagcac tgcatcgggc
     4441 ttttgtcctg acagcagctg aaaaacgcca tcagcaataa agaagacggc cagatcgtca
     4501 gttaatgcgg aagttgccag taaagcatct aaaccttccc gaccagcagc tgtaccatga
     4561 ggtgcagtag aaaaaacaaa cgcaattcgt ttcatcagaa ctgtaccacc ctgtcacagg
     4621 tcagcgaggc ttccgccagc gcgccaagtc cgcttaaggt aaatcccggc tgaaggtttg
     4681 acgaagccag ccccagcctt cctgcttcag tttcatcgac gataccgcgg cgtaacgctg
     4741 ccgctacgca gatattcagc gccacaccat gttgcgcatt caactgttgc cagccgcgca
     4801 caaggtcaaa ttcgtcgctt gccggtgagg tcagttggtt agcgttatag accccttccc
     4861 gatagaaaaa gacgctgctt aacttatggc cttctgctat cagcgcctgc gcaaactgaa
     4921 aagcactact cgcctgttgc gtaccgtatg ctggcccggt caccacgatg gcaaaacgca
     4981 ttacttatct tgcccctgaa aatcaccgct cttgaactgg cggatgtaga gatagacagt
     5041 gtgtttggag atgttcaggc ggtcagcaac ctggttgatc gcatctttaa tatcgaagat
     5101 ccctttctcg tagagattca gcacgatctg acgatttttg gcgttattag aaacattgcg
     5161 atcggcattc acttcttcga tggtgaactc cagcgtttgg gtaaccagat cttcaacaga
     5221 agaggcaaag ttgacgcttg aaccgacatc cggggtttct ggcggcacaa aagtgctcat
     5281 aatctgcgag aagggaacat caagattcat attgatgcac agcagaccaa ttacacgctg
     5341 ttcgcggtta cgaatcgcga tagtcaggga cttcattaat acgccgcttt tggcgcgagt
     5401 aaagtagcat ttagaaacgc tgctatccgc tcccgtcata tcgtgcagca tacgtagcgc
     5461 caggtcagta attggcgaac caatcttccg gcctgtatgt tcaccgttag caatgcgaat
     5521 ggctgaacat tttagatcct gcaaagagtg caaaacgatt tcacagtggg agccaataag
     5581 catcgctaac ccgtccacca ccgcttcgta ggatttcaga atatcaaaat cggtctggtc
     5641 gaaaggacgt tgatccagta aatccaactc actggtttcg ttggttaaaa gcgacctgga
     5701 catgaaaaaa aacactcctt ttcaggagcc tgtcgttaac ttttcagggc aggctcatta
     5761 atgatgcggg taactaaatt aatacagcgg aggttccgct ttccagcact aattatatcc
     5821 ggcctgtaat aaaaaaaccg ccgcctggtc aggcggcggt tcttaatgct tattttttag
     5881 cagaatctgc ggctttcgca tcagcttccg gctttgcatc agccttcggc gctggtttca
     5941 catccagcag ctctacgtca aacactaggg tagaattcgg tgggatcccc ggaacacccg
     6001 ctttgccgta agccagttct ggtggaataa ccagtttgat cttaccgcct ttcttgatgt
     6061 tcttcagacc ttctgtccaa cccgggataa caccgtccag acggaaagaa agcggttcac
     6121 cacgggtgta agagttgtcg aactctttac cgtcgatcag cgtacctttg tagttcacta
     6181 caacagtatc gctgtctttc ggtgcttcgc ctttaccggc ttctactacc tgataaacca
     6241 gaccagttga agaggttttc acacctttct ctttggcaaa tttctcgcgg tactctttac
     6301 cttttgcttc gttatcagcc gcgtcttttt ccatcttcgc ctgagcagaa gacttcacgc
     6361 gagcttcgaa tgcttgcaga gtctgttcga tctcttggtc ggagagtttg ctcttatcag
     6421 caaatgcatc ctgaacacca gcgatcagct gatctttatc cagtttgatg cccagttttt
     6481 cttgttcttt aagagagttt tccatgtaac gacccagcga agcacccagt gcataagctg
     6541 atttctgatc gtcatttttg aacgatgctt tgctgtctgc agttgtagca ggttttgcag
     6601 cttcagcagc aaaagtgatt ggtgcatgca gggcaacggc cattgtggtc gccagtagcg
     6661 ttactttaaa cagtgatttc atccatatct ccaggatcgg ggcatctcac cccagggtta
     6721 actattatca gaagggtact ataaagcgtt gtcgaacaaa tctacataca gacacgccct
     6781 attatcatct attttcagac tctttttgtt taaattagtt tcgatgaccg cgaaatgagt
     6841 gctgtctcgg gcagcaaagt taagtagaat ccgcggcgac cattcgacaa aagaggtgaa
     6901 tcatgcagga tttatcattg gaagcacgcc tggcagagct ggagagccga ctggcttttc
     6961 aggaaatcac cattgaagaa ctgaacgtca cggtggccgc tcatgaaatg gagatggcga
     7021 aactgcgcga tcatctgcgt ctgctgaccg agaagttaaa agccagccag ccgtcgaaca
     7081 tcgcgtcgca ggctgaagaa acgccaccgc cacattattg aggcgtaaaa aaagcgggga
     7141 ttccccgctt ttttgtcacc ttttcggtat tagtggcaac cacaaccgcc gttgccttta
     7201 ccgccacagc agccttcgcc accgtgttca tgaccgtgat cgtggccatg accgccgcag
     7261 caaccgtcgt ggtcgtgatc gtggtgatga tcgtgcgcgc cgtgaacgtg gccatgagcc
     7321 agttcttctt cggtcgcttc gcgaatcgcc acaacttcaa cgttgaattt caggttctga
     7381 ccggccagca tgtggttacc atcaaccact acgtgatcgt cttcaaccgc agtgatttca
     7441 accggtaccg gaccctggtc agtttcagcc aggaaacgca tacctacctg cagttcatca
     7501 acgcccataa atacgtcttt aggaacacgt tgcactaggt tttcgtcgta ctgaccgtaa
     7561 gcgtcgttcg cgccaacagc gacatcaaat ttgtcgccaa cttcatgacc ttccagcgcc
     7621 gtttccaggc cagagatcag ggaaccgtga ccatgcaggt agtccagcgg cgcactcacc
     7681 ggagactcat caaccaacac accgtcttct gtacgtacct gataggccag gctgaccacc
     7741 aggtcttttg ctactttcat gatatctcct gagcatggga agaatagtgg cgcagattgt
     7801 agcggaattc tgcagccgtg tactcactag cttaaaaaaa ctcggcgcat atcgctagtc
     7861 cggatgaaaa atcccgatca cttgctcatc tttgtgaacg tgatcgcggg cttctttgtc
     7921 tgcttctcgc atctgatgtc cgcacttaac acattcaaca atatcaatat tattttcgcg
     7981 ccacatcgcc attgaatcct gcgcctgaca ggccgggcat tttgcgcccg ctataaaacg
     8041 ttttcggatt gccatcctta ccctctactc aaattcatcc cagccgtcca gctggcgtcg
     8101 ttcttgttgc atttcacgct ggaaaatctc ttccagttcg cgtcgggttt ccctggcgcg
     8161 agaaatttgt acggtatcag catgcatcgg gattaactcc cgcagcattc gcatatccag
     8221 gcggcgaaaa tgcagttgcg cccgctgtgc ctgatgcgga tgcatgccaa gcgtgaccag
     8281 cgtcttgcgc cccagctcta acgcactgga gaatgtttca cgggaaaact gcgtcacccc
     8341 tgcctgtaat aactcatgcg cttccacacg tccgcgcgct cgcgcaagaa tatgcaaatg
     8401 cggaaagtgc tgctggcata tttccaccag cttcatggtg tcttccggct cgttacaggt
     8461 aatgacgata gactcagcgg cctctgcacc cgcagaacgt aaaagatcga cctgcgtggc
     8521 gtcgccgtaa taaactttgt agccatattt acgcatcagg ttaacggcgc tgatatcccg
     8581 ctccagcacg gtaatgcgca ttttatttgc catcagcaaa cgaccaatca cctgaccaaa
     8641 acgcccgaag cccacgacaa tgacctgggg tttatcatcg ttgacccacg gtttttcgtc
     8701 ttcttcttcc ggtccgttaa actggcggga tagccattta tccaccagct tcatcagcaa
     8761 cggcgccatc tggtcgccct ggaataaacg ttgtgaagaa gcggtagaaa agaggacaaa
     8821 ggcaaactca ccgccctgac tcaacacgcc agcaaactgc atccgctctg agctacgcac
     8881 gccatacaat cgcgccagca gatacagcac gagaattttc accgccacca gcacaaccac
     8941 gcttatcact acccacaaca gatgggtata aagcaccccg aggttgagcg acatgccgac
     9001 agagataaag aacaaaccga gcagcaagcc tttgaaggga tcgatagccg tttccagttc
     9061 atggcgatat tcactttccg ccagcagcac gcccgcaata aatgtaccga gcgccatcga
     9121 cagccccagc gcatccataa acaatgcgga acccaacacc agcagcagcg tcgcggcggt
     9181 gaacacttcc cgcacgccag aagctgcaat aaagcggaat accggacgca gtaaatagcg
     9241 cccaccaatc agcatgccga caaactccag caccttcatg ccgatcttca tccagtcgaa
     9301 atgttcgtct gccgaccccg ccaacaacgg cactaacgcc agtgctggga tcactgccag
     9361 atcctgaaac agcagaaccg aaaacccgag ctggccggat tcgctgcgat tcatcccttt
     9421 ctcacgcatc aattgcaacg ccattgcagt tgaagacatc gcaaagccaa tgccaccgac
     9481 caccgccgcc tgccaggcga aatccgtcag catcaataat cccgccagca acgccgcgct
     9541 taacagcacc tgtgccgcgc ctacgccaaa aatcgaacgc cgcagttgcc aaagtttgga
     9601 gggattcaac tcaaggccga tgataaacat caggaatacc acgccgagtt ccgaaaagtg
     9661 gaggatctca tcgacgtcgc taataaaccc cagcccccac gggccaattg caatccccgc
     9721 cagcaaatat cccaacacag cgccaatccc cagccgcgaa gagaaacagc actcctgcga
     9781 gtaaaaaatc ggaaccttcc atcagcggcc tcctggagac agcggatttg ccaaccagtc
     9841 accgtaggct ctggcgtggc tcgccagctc ctttgcgctt tgccgtctcg cccagtaaat
     9901 aatgatggga cttaaccaat gcatccggca catgcccgcc gccagttcaa aggggcgcag
     9961 cacatcgctc atcgggtagc gattcagcgc gtcataacgg taagcacttt ccggctcgcc
    10021 ggtggtaatc acgttacgcc agtactttcc cgccagttgg tttcctcccg gcccgctggc
    10081 aaaaccacga cttaataccc ggtccagcca ctctttcagt agcgccgggc agctataggt
    10141 ataaagagga tgctgaaaga caatcacctc gtgctcgcgc agtaatgcct gctcacgggg
    10201 gatatcaata aaaaaatcgg gatagtgcgc gtaaaggtcg tgcacggtaa cattgctgag
    10261 ctgcgtggcc ggtttaagca gtacccggtt tgccaccgag tcctgagatt ccggatgggc
    10321 atacagcagc aaaactttcg ctggctgaga catcatcccc ctcccggtat ggtttttgtg
    10381 tatagtcgct gttttgggct accattgcgc ccggtgcggc agctcgccca tacattacat
    10441 tatcataatg ataagttaac atagtctgaa catacggcgc cttatgattg ttttctcctc
    10501 gttacaaatt cgtcgcggcg tgcgcgtcct gctggataat gccaccgcca ccatcaaccc
    10561 cgggcagaaa gtcggcctgg tgggtaaaaa cggctgtggt aaatctaccc tgctggcatt
    10621 gctgaaaaat gaaatcagcg ccgacggcgg cagctacacc tttccgggaa gctggcaact
    10681 ggcgtgggtg aatcaggaaa cgccagcgtt accgcaagcg gcgctggaat atgtcattga
    10741 cggcgaccgt gaatatcgtc aactggaagc gcagctgcac gacgccaacg aacgtaacga
    10801 cgggcacgcc attgcgacca ttcatagcaa gctggatgct attgacgcat ggagtattcg
    10861 ctcccgtgcc gccagcctgc tgcacggcct cggtttcagc aatgaacaac tggagcgccc
    10921 ggtaagtgat ttctccggtg gctggcgtat gcgtcttaac cttgcccagg cgctgatttg
    10981 ccgttcagac ttgctgctgc tcgacgaacc gactaaccac ctcgatctcg atgccgttat
    11041 ctggctggaa aaatggttga agagctatca gggcacgctg atcctgatct ctcacgaccg
    11101 cgacttcctc gatccgatcg ttgataaaat tattcatatc gaacaacaaa gcatgttcga
    11161 gtacaccggc aactacagtt cgtttgaagt acagcgcgcc acccgtctgg cgcagcaaca
    11221 agcgatgtac gaaagccagc aggaacgcgt ggcacatctg caaagttata tcgatcgttt
    11281 ccgtgccaaa gccaccaaag cgaagcaggc ccagagccgc attaagatgc tggaacgtat
    11341 ggagctgatt gcccccgcac acgtcgacaa cccgttccgc tttagcttcc gcgcgccgga
    11401 aagcctgcca aatccgttac tgaagatgga aaaggtcagc gcaggctatg gcgatcgcat
    11461 tattctcgac tcgattaaat tgaatctggt ccccggctcg cgcattggtc tgttaggccg
    11521 caacggcgca ggtaaatcga cattaatcaa actgttagcc ggtgaacttg cgccagtcag
    11581 cggtgaaatt ggtctggcga aagggatcaa gctcggctac ttcgcccagc atcaacttga
    11641 atacctgcgc gccgacgaat cacctattca acatctggca cgtttagcgc cgcaggagct
    11701 ggaacaaaaa ctgcgtgact acctcggcgg ctttggtttc ctgggcgata aagtaaccga
    11761 agaaacgcgc cgcttctccg gtggggaaaa agcccgcctg gtgctggcat taattgtctg
    11821 gcagcgtccg aatctgctgc tgctcgacga accgactaac caccttgacc tcgacatgcg
    11881 tcaggcactc accgaagcat taatcgagtt tgaaggcgcg ctggttgtcg tttcgcacga
    11941 ccgtcatttg ctgcgttcca ccactgacga tctctacctg gttcacgatc gtaaagtcga
    12001 accgttcgac ggcgatctgg aagattatca acagtggttg agcgacgtac aaaagcagga
    12061 aaaccagacc gacgaagcgc caaaagagaa cgcgaacagc gcccaggcac gtaaagatca
    12121 gaagcgtcgg gaagcggagc tgcgtgcgca aacccagccg ctgcgtaaag agattgcccg
    12181 tctggaaaaa gagatggaga agctgaacgc gcaactggcg caggcggaag agaaactcgg
    12241 cgacagcgaa ctgtatgacc agagccgtaa agcggagttg accgcctgcc tgcaacagca
    12301 agccagcgcc aaatccggcc tggaagagtg cgaaatggcg tggctggaag cccaggagca
    12361 gcttgagcag atgctgctgg aaggccaaag caactgatgg cgcagataac gacgaccgat
    12421 gccaatgaat ttagcagcag tgctgaattc acccctatgc gcggctttag caattgtcat
    12481 ctacaaacta tgctgccgcg tttgtttcgt cgtcaggtga aattcacccc gtactggcag
    12541 cggctggagt tgcccgacgg cgattttgtc gatctcgcgt ggagtgaaga ccccgcacag
    12601 gcgaaacata aaccgcgttt agtggtgttt cacgggctgg aaggcagtct caatagccct
    12661 tacgcccacg gtctggttga ggcggcgcaa aagcgcggct ggctgggcgt ggtgatgcat
    12721 tttcgcggat gcagcggtga accaaaccgt atgcaccgca tttaccattc gggcgaaacc
    12781 gaagacgcca gttggttttt acgctggctg caacgcgaat ttggtcatgc gccaacggct
    12841 gccgtcggct attcgctcgg cggtaatatg ctggcctgtt tgctggcaaa ggaaggcaat
    12901 gatctcccgg ttgatgcggc ggtgattgtc tctgcgccgt ttatgctgga agcctgtagc
    12961 tatcatatgg aaaagggctt ttcccgcgtt tatcagcgtt acttgctgaa cctgttaaaa
    13021 gccaatgccg cgcgcaagct ggcagcctac cccggaacgc tgccgattaa tctcgcgcag
    13081 ttaaaatcgg tacgtcgcat ccgtgaattt gacgatctaa tcaccgccag aattcacggc
    13141 tacgccgacg ctatcgacta ttatcgtcag tgtagcgcca tgccaatgct gaaccggatc
    13201 gccaaaccga cgctgattat tcacgccaaa gacgatccgt ttatggatca tcaggtgatc
    13261 ccaaaaccgg aaagtctccc ctcgcaggtg gagtatcaac tgactgaaca tggcggtcat
    13321 gttggcttta ttggcggtac attacttcat ccgcaaatgt ggctggagtc acgcattcct
    13381 gactggttaa caacgtatct ggaggcgaaa tcatgttgat tccgtggcaa gacctctccc
    13441 ccgaaacgct ggaaaattta attgaaagct ttgtgttacg tgaaggcacc gattatggtg
    13501 aacatgagcg tacacttgaa cagaaagtcg ccgacgtcaa acgccagcta cagtgcggag
    13561 aagcggtgct ggtatggtcg gagctgcacg aaacggtcaa tattatgccg cgcagtcagt
    13621 ttcgcgaata acctgcaacc gtcggcggac gagataatag tgatagctaa gtcatggagt
    13681 tactatgtct gccaaacatc cggtcattgc ggtaacagga tccagcggcg cggggaccac
    13741 caccaccagc ctcgcgtttc gtaaaatatt cgcgcagtta aatctgcatg cagctgaggt
    13801 ggaaggcgac agttttcacc gttacacccg cccggaaatg gacatggcga tccgcaaagc
    13861 gcgcgacgcc gggcggcata tcagctactt cggccccgaa gcgaacgact tcggcctgct
    13921 ggaacaaacc ttcattgaat acggtcagag cggcaaaggg aaatctcgca aatatctgca
    13981 tacctacgac gaagccgtac cctggaatca ggtaccggga acattcaccc cctggcaacc
    14041 tttaccagaa cccactgatg tactgtttta tgaaggttta cacggcggcg tagtcacgcc
    14101 acagcataac gttgcgcagc atgtggactt actggtcggc gtagtgccta tcgttaacct
    14161 tgagtggatt caaaaactga tccgcgacac cagcgagcgc gggcactcac gagaagcagt
    14221 gatggactcg gtagtgcgtt caatggaaga ctatatcaac tacatcacac cgcagttttc
    14281 ccgcacccat cttaacttcc agcgcgttcc caccgtcgac acttcaaacc cgttcgcggc
    14341 aaaaggtatc ccgtcgctgg atgaaagctt tgtggtgatc cattttcgta atctggaagg
    14401 gatcgatttc ccttggctgc tggcgatgtt gcaaggctca ttcatttccc acatcaatac
    14461 gttagtggta cctggcggca aaatggggct ggcaatggaa ttaattatgc tgccgctggt
    14521 gcaacgattg atggaaggaa agaaaatcga gtaactttgc tattatgccg gataaaatac
    14581 tatccggctt cacaacggga tagttaagtc acgcggcaac cacttcatac gagtgagtaa
    14641 tattcaccgc tttttccagc atcaacgcca ctgaacaata tttctcggca gagagatcaa
    14701 ccgcacgcgc aaccgctgcg tctttcaggt cgcgaccggt gacgataaaa tgcagattaa
    14761 tgtgcgtaaa caggcgtggt gcctcttcgc ggcgttcaga ggtcaatttt acttcacaat
    14821 cgaccacatc ctgacgccct ttttgcagga tcgaaaccac atcgatggca ctgcaaccac
    14881 ccgccgccat caacaccatt tccatcggac ttggtgcttt atcgcctgag ttgccgtcca
    14941 ttaaaatctg gtgaccagag gcggattcgc ccagaaaagt taacccttcg acccacttca
    15001 ctcgcgcttg catttttgct actccactgc gtcaattttc ctgacagagt acgcgtacta
    15061 accaaatcgc gcaacggaag gcgacctggg tcatgctgaa gcgagacacc aggagacaca
    15121 aagcgaaagc tatgctaaaa cagtcaggat gctacagtaa tacattgatg tactgcatgt
    15181 atgcaaagga cgtcacatta ccgtgcagta cagttgatag ccccttccca ggtagcggga
    15241 agcatatttc ggcaatccag agacagcggc gttatctggc tctggagaaa gcttataaca
    15301 gaggataacc gcgcatggtg cttggcaaac cgcaaacaga cccgactctc gaatggttct
    15361 tgtctcattg ccacattcat aagtacccat ccaagagcac gcttattcac cagggtgaaa
    15421 aagcggaaac gctgtactac atcgttaaag gctctgtggc agtgctgatc aaagacgaag
    15481 agggtaaaga aatgatcctc tcctatctga atcagggtga ttttattggc gaactgggcc
    15541 tgtttgaaga gggccaggaa cgtagcgcat gggtacgtgc gaaaaccgcc tgtgaagtgg
    15601 ctgaaatttc gtacaaaaaa tttcgccaat tgattcaggt aaacccggac attctgatgc
    15661 gtctgtctgc acagatggcg cgtcgtctgc aagtcacttc agagaaagtg ggcaacctgg
    15721 cgttcctcga cgtgacgggc cgcattgcac agactctgct gaacctggca aaacaaccag
    15781 atgctatgac tcacccggac ggtatgcaaa tcaaaattac ccgtcaggaa atcggtcaga
    15841 ttgtcggctg ttctcgtgaa accgtgggac gcattctgaa gatgctggaa gatcagaacc
    15901 tgatctccgc acacggtaaa accatcgtcg tttacggcac tcgttaatcc cgtcggagtg
    15961 gcgcgttacc tggtagcgcg ccattttgtt tcccccgatg tggcgcagac tgatttatca
    16021 ccccgatatc aactatgcac ttcgacaaac gctggtgcta tgtttgcccg tggccgttgg
    16081 gttaatgctt ggcgaattac gattcggtct gctcttctcc ctcgttcctg cctgttgcaa
    16141 tattgcgggc cttgatacgc ctcataaacg ttttttcaaa cacttaatca ttggtgcgtc
    16201 gctgtttgcc acctgtagct tactgacaca gctgctactg gtaaaagatg ttcccctgcc
    16261 ctttttgctg accggattaa cgctggtact tggcgtcact gctgagctgg ggccattaca
    16321 cgcaaaattg cttcctgcat cgctgctcgc cgccattttt accctcagtc tggcgggata
    16381 catgccggtc tgggaaccat tgctcatcta tgcgttgggc actctctggt acggattgtt
    16441 taactggttt tggttctgga tctggcgcga acaaccgctg cgcgagtcac taagtctgct
    16501 gtaccgtgaa ctggcagatt attgtgaagc caaatacagc ctgcttaccc agcacaccga
    16561 tcctgaaaaa gcgctgccac cgctgctggt gcgccagcaa aaagcggtcg atttaattac
    16621 tcagtgctat cagcaaatgc atatgctttc cgcgcaaaac aataccgatt acaagcggat
    16681 gctgcgtatt ttccaggagg cgctggattt gcaggaacat atttcggtca gtttgcatca
    16741 gccggaagag gtgcaaaaac tggtcgagcg tagccatgcg gaagaagtta tccgctggaa
    16801 tgcgcaaacc gtcgccgctc gcctgcgcgt gctggctgat gacattcttt accatcgcct
    16861 gccaacgcgt tttacgatgg aaaagcaaat tggcgcactg gaaaaaatcg cccgccagca
    16921 tccggataat ccggttgggc aattctgcta ctggcatttc agccgcatcg cccgcgtgct
    16981 gcgcacccaa aaaccgctct atgcccgtga cttactggcc gataaacagc ggcgaatgcc
    17041 gttgcttccg gcactgaaaa gttatctgtc actaaagtct ccggcgctac gcaatgccgg
    17101 acgactcagt gtgatgttaa gcgttgccag cctgatgggc accgtgctgc atctgccgaa
    17161 gtcgtactgg atcctgatga cggtattgct ggtgacacaa aatggctatg gcgcaacccg
    17221 tctgaggatt gtgaatcgct ccgtgggaac cgtggtcggg ttaatcattg cgggcgtagc
    17281 gctgcacttt aaaattcccg aaagttacac cctgacgttg atgctgatta ccaccctcgc
    17341 cagctacctg atattgcgca aaaactacgg ctgggcaacg gtcggtttta ctattaccgc
    17401 agtgtatacc ctgcaactgt tgtggctgaa cggtgagcag tacatccttc cacgtcttat
    17461 cgataccatt attggttgtt tgattgcttt cggtggtacc gtctggctgt ggccgcagtg
    17521 gcagagcggg ttattgcgta aaaacgccca tgatgcttta gaagcctatc aggaagcgat
    17581 tcgcttgatt ctgagcgagg acccgcaacc aacgccactg gcctggcaga gaatgcgggt
    17641 aaatcaggcg cataacactc tgtataactc attgaatcag gcgatgcagg aaccggcatt
    17701 caacagccat tatctggcag atatgaaact gtgggtaacg cacagccagt ttattgttga
    17761 gcatattaat gccatgacca cgctggcgcg ggaacaccgg gcattgccac ctgaactggc
    17821 acaagagtat ttacagtctt gtgaaatcgc cattcagcgt tgtcagcagc gactggagta
    17881 tgacgaaccg ggtagttctg gcgatgccaa tatcatggat gcgccggaga tgcagccgca
    17941 cgaaggcgcg gcaggtacgc tggagcagca tttacagcgg gttattggcc atctgaacac
    18001 catgcacacc atttcgtcga tggcatggcg tcagcgaccg catcacggga tttggctgag
    18061 tcgcaagttg cgggattcga aggcgtaata caggctaaac catcggataa ggcattcacg
    18121 ccgcatccga catattttgc ctgatacgtc ttatttacgc cccaaccacc ttcgccaccg
    18181 cgtgggcgaa acgttgcatc ccttcatcga tatccgcatc ttccaccacc agcgacggtg
    18241 ctaaacgcat cacatccggt ccggcattca gcaccattac gccaacctct gcacccgcat
    18301 acaggaaatc acgcgcccga cctttgtagt gtggtttcag ctccgcgcca attaacagcc
    18361 ccataccgcg aatatcgcta aacacatcgt actgctgatc gatcttctgc aaatggtcaa
    18421 caaaacgctg gcgtttcgcc tgaatgcctt ccagcacttc aggggtattg atgatatcaa
    18481 acgccgcgcc cgctactgca caggccagag gattaccgcc gtaggtggaa ccgtgagaac
    18541 caggatgaaa cgcagaagca atttccgccg tggtcagcat ggcgctaatc gggaagccgc
    18601 cgcctaacgc tttcgcagaa gtcagaatat ccggcgtcac accgtagtac ctgtaagcaa
    18661 acaaatcgcc agtccgcccc atcccgcact gcacttcatc aaacaccaat aatgcctgat
    18721 gttgatcgca cagctcgcgc aagccctgca aaaactctgg cgtcgctgcc gtcacaccgc
    18781 cctcgccctg gatcggctca accaccaccg cacaggtgtg atcatccatc accgctttca
    18841 ctgcatggag atcgttaaag ggaacgtgga tgatgtctgc cggtttcggc ccaaagccgt
    18901 cggaatattt tggctgccca cccaccgaaa cggtaaacag cgagcgacca tgaaaagcgt
    18961 tatggaaggc aataattttg gttttgaacg ggctatgacg cacacaggcg taatggcgtg
    19021 ccagtttaaa ggcggtttcg ttagcttccg tgccggagtt cataaacacc acgcgttcgg
    19081 caaacgtcgc ctcaatcagt ttgcgcccaa gacgcagcgc cggttcattg gtgaaaacgt
    19141 tactgatatg ccacagagtt tcgccctggg tttttaacgc gttcaccagc gcaggatggc
    19201 aatggcccaa cgccgtaact gcaatgccac ccgcgaaatc gacatactcc ttgccttgct
    19261 gatcccagat tcggctgccc tgacctttta ccggaataaa ctctgccgga gcataaatcg
    19321 gcaggatcac ttcatcgaaa gtcgcgcgtg taattgctgt ttgttcaatt gccatctcat
    19381 gatcaccctg ttacgcataa ataaatgtga aattataatc acaaaatatg cataaaaaat
    19441 cactgaatgg caatcggaaa tcagagatgc aggaaattag ccagcagttg atgtccttgt
    19501 tcgctaagaa tactttctgg atggaactgc acaccttcca gatcccactg gcgatggcga
    19561 atccccataa tctcgcgggt ttcgctccag gccgtcacgt caaagcacgc tggtaatgag
    19621 tcaggttcca ccaccagcga atggtagcgt gtcacggtaa gtggatttgc cagcccccga
    19681 aatacgccct caccgctatg tgtaatcggc gaggttttgc cgtgcatgac ctttgcggcg
    19741 cgcacaactt tgccgccaaa tgcctgcgcc attgcctgat gaccgaggca gacgccaaga
    19801 atcggcaagc gcccggcata gtggcgaata acgtcaaggg agatcccggc ttcatctggc
    19861 gtacaggggc caggtgagat gacaattttt tgtggtttaa gggcgtcgat atccgccagc
    19921 gtcaacgcat cgttgcgctt aaccagcaca tccgccccca gttcacaaaa gtactggtag
    19981 aggttccagg taaaagaatc gtagttatct ataagcagga tcatggcggc tccggtacga
    20041 aagaaccgcg ctattttact cagattcccc ggcttcgctt accactttgc taaatatcat
    20101 ctgcagtgcg gtgagatccc ccattgcccc actctgattt gcctgattcc aggcctcttt
    20161 ttcgataccc tgccagctca gttgatatcc ggcatgaatc gccagttgct cgaagaagat
    20221 ccgctgtgcc agaccacttc ccacccggaa gggatgcagc acgttgattt cacaatagta
    20281 atgcgccagc cgctcgacga acttcgcttt ctccaggcca accagataac cttcttcctc
    20341 cagatcctgc atcagggcat tgccctcttt ttcgatataa gcaaagtggc agaacggcgt
    20401 atcgccctga taaatatcaa cttcacgcag ttgccctgcc cagtcgaaaa tatcctggta
    20461 cagctggcga tggatagtgc gcaaatgcgg taaaccgcgc accagcggac caagctcaat
    20521 ggtcgcagca cgcagcgccg tcatttcgta agcggcctgt tccagccgct gctgctggcg
    20581 gatgttcagc cggttacgca tgatatcaag gccaggataa agatacagat cgcgcccttc
    20641 gccgaattta tcgctcatag tgcctcctca gctcttcaag gcgcgctaaa gcctctgcgg
    20701 cagtaagagt gactaaaggc atctcgacgc cttcaagacg gcgactggcc tggaaattac
    20761 gattacgctg aagctcccag agacgggact tttgcttatc ggtgagtttc ttcacttgat
    20821 gcctccctga acgtgtccat ttgccacaag tataagcggc aaatccgggt tacgccggga
    20881 gagcagaagc gcgggcaaga ttgcccgcgc gagaaatcat tacggcagga ctttagcgga
    20941 aaggataact accggttttg acggcacatt ctggtacgga ccaacatcat gagtcggcac
    21001 ctgggaaatc ttatcggcaa cgtccatgcc tttcaccact ttaccaaata ccgcgtaacc
    21061 gaaatcacgc tgaccatggt caaggaaggc gttatcggca acgttgataa agaactggct
    21121 ggtggcgctg tctttgtcag cggtacgcgc catcgcgatg gtgccacgcg tgttgcgcag
    21181 gccgttatcg gcttcatttt tgattggcgg gtttggtttt ttctgctgca tctgctcggt
    21241 gaaaccgccg ccctgaatca taaagccagg aatgacgcgg tgaaaggtag tgttgttata
    21301 aaaaccgctg ttcacataat cgacaaagtt ttgcacagac actggcgctt tttgtttatc
    21361 cagctccagt tcgatgttac cagctgaggt tgtcaacaat acgtgcgggt cccctttcgc
    21421 tgccattgct gcgggagaaa gagcagaaag agcagaaaga gcagaaagag cgaaaacagc
    21481 agccatcgcc gccagggtcg atttgaacat gagatttcct taacaaacag agcagaaaaa
    21541 aagcaagtga agagattcta aatagccttc aagaacaagg ccatcccttt acctaatttt
    21601 acgtatctga aatatatgta acctgacgat cactttttat tccgtgatca aaatcacctc
    21661 ttaaaatgca atttagcaac cgattgcaat aaaacattta aacagatcat aaaatcacct
    21721 aaaatcgccc gctcgcggca tatcccgcta tgccatttta tttttgaacg caggccaatc
    21781 atgactaaca gcaatcgcat caagctcaca tggattagct ttctctccta cgcactgact
    21841 ggtgcgttgg ttattgtcac cgggatggtg atgggaaata tcgccgatta tttcaatctg
    21901 cctgtttcca gtatgagtaa caccttcacc ttcctcaacg ccggcatttt aatctctatc
    21961 ttcctcaacg cctggctgat ggaaatcgtc ccgttgaaaa cgcagttacg ttttggcttt
    22021 ctcctgatgg tgctggcggt tgccggtttg atgttcagcc acagcctggc gctgttctcg
    22081 acggcgatgt tcattctcgg ggtggtcagc ggcattacta tgtcgattgg tacattcctg
    22141 ataacacaaa tgtatgaagg gcgtcagcgc ggttcccgcc tgttatttac tgactccttc
    22201 ttcagtatgg ctgggatgat tttcccaatg atcgccgcgt ttctactggc gcgcagcatt
    22261 gagtggtact gggtttatgc ctgcatcggg ctggtgtatg tcgctatttt tattctgacc
    22321 ttcggctgtg agttcccggc actgggcaaa cgtgcaccaa aaacggatgc tccggtagag
    22381 aaagaaaagt gggggatcgg cgtactgttt ctctctgttg cggcgctgtg ctacatcctc
    22441 ggtcagttag gttttatctc ctgggtgcct gagtatgcca aaggcctggg catgagcctg
    22501 aacgacgcgg gcacgctggt gagtaacttc tggatgtcat acatggtcgg catgtgggcg
    22561 ttcagcttta ttcttcgctt ctttgatttg caacgcattc tgaccgtact ggctggtctg
    22621 gctgcgattc tgatgtacgt ctttaacacc ggaacaccag tacatatggc gtggtcaatt
    22681 ctcgctctgg gcttcttctc cagcgcgatc tataccacca tcatcaccct gggttcacag
    22741 cagaccaaag taccgtcgcc aaaactggtt aactttgtcc tgacctgcgg aaccatcggt
    22801 actatgttga cctttgtggt taccggcacg attgttgaac atagcggtcc gcaggcggca
    22861 ctgcttacag caaacggtct gtacgctgtc gtctttgtga tgtgcttcct gttaggtttc
    22921 gtcagccgtc accgtcagca taacaccctg acctctcatt aattgctaat gccggacggc
    22981 actatcgtcg tccggccttt tcctctcttc ccccgctacg tgcatctatt tctataaacc
    23041 cgttcatttt gtccattttt tgcacaaaca tgaaatgtca gacaattccg tgacttaaga
    23101 aaatttatac aaatcagcaa tatacccctt aaggagtata taaaggtgaa tttgatttac
    23161 atcaataagc ggggttgctg aatcgttaag gtaggcggta atagaaaaga aatcgaggca
    23221 aaaatgagca aagtcagact cgcaattatc ggtaacggta tggtcggcca tcgctttatc
    23281 gaagatcttc ttgataaatc tgatgcggcc aactttgata ttaccgtttt ctgtgaagaa
    23341 ccgcgcatcg cttatgaccg cgtacacctc tcatcttact tctctcacca caccgccgaa
    23401 gagctgtcgc tggtgcgcga aggcttctac gagaaacacg gcatcaaagt tctggtcggc
    23461 gaacgcgcta tcaccatcaa ccgtcaggag aaggtgattc actccagcgc cggacgtacc
    23521 gttttttatg acaagctgat catggcaacc ggttcctacc cgtggatccc accaatcaaa
    23581 ggttctgata ctcaggactg ctttgtctat cgcaccattg aagacctcaa cgccattgaa
    23641 tcctgcgccc gtcgcagcaa acgcggtgcc gttgttggtg gcggcctgtt aggtctggaa
    23701 gccgctggcg cgctgaaaaa cttaggtatt gaaacccacg ttatcgaatt tgcccctatg
    23761 ctgatggcgg aacagcttga tcagatgggt ggcgagcagc tgcgtcgcaa aatcgaaagt
    23821 atgggcgtgc gcgttcacac cagcaaaaac acccttgaga ttgtgcagga aggtgtcgaa
    23881 gcgcgtaaaa ccatgcgttt tgccgacggc agcgaactgg aagtcgactt tatcgtcttc
    23941 tctaccggta tccgtccgcg cgataagctg gcaacccagt gtggtctgga cgttgctccg
    24001 cgtgggggga ttgtcattaa tgattcctgc cagacttccg atccggatat ctacgccatc
    24061 ggtgaatgcg caagctggaa caaccgtgta tttggtctgg tagcaccagg ctacaaaatg
    24121 gcgcaggtcg ccgttgacca tattctgggt agcgaaaacg cctttgaagg tgctgacctt
    24181 agcgccaagc tgaaactgct gggcgtagac gtaggtggta ttggtgatgc gcacggtcgc
    24241 acgcctggcg cacgtagcta tgtttacctc gacgaaagta aagagatcta caaacgcctg
    24301 attgtcagcg aagacaacaa aaccctgctc ggtgcggtac tggtgggcga taccagcgac
    24361 tacggcaacc tgctgcaact ggtgctgaac gctatcgagc tgccggaaaa cccggattct
    24421 ctgattctgc cggcgcactc gggtagcggt aagccgtcta tcggtgttga taaactgccg
    24481 gacagcgcgc aaatctgctc ctgcttcgac gtcactaaag gtgatctgat tgctgccatc
    24541 aacaaaggct gccacacagt tgcggcgctg aaagctgaaa ccaaagcggg tactggctgc
    24601 ggtggctgta tcccgctggt cactcaggta ctgaacgcgg aactggcgaa acagggcatc
    24661 gaagttaaca acaacctgtg cgaacacttt gcttattcgc gtcaggaact gttccatttg
    24721 atccgcgttg aaggcattaa aaccttcgaa gaactgctgg cgaaacacgg caaaggctac
    24781 ggttgtgaag tttgtaaacc aaccgtcggt tcgctgctgg cctcctgctg gaacgaatac
    24841 attctgaagc cggaacatac tccgctgcag gattctaacg acaacttcct cgctaacatc
    24901 cagaaagacg gcacctactc ggtgatcccg cgttctccgg gcggtgaaat caccccggaa
    24961 gggctgatgg cagtgggtcg tatcgcgcgt gaatttaatc tctacaccaa aatcaccggc
    25021 tcccagcgcc tggcgatgtt tggcgcacag aaagacgatc tgccggaaat ctggcgtcag
    25081 ctgattgaag ctggcttcga aaccggtcat gcctatgcga aagcactgcg tatggcgaaa
    25141 acctgcgtgg gtagcacctg gtgccgctac ggtgttggcg atagcgtcgg cctcggcgtg
    25201 gaactggaaa accgctacaa aggcatccgt acgccgcaca aaatgaagtt cggtgtctcc
    25261 ggctgtaccc gtgaatgttc agaagctcag ggtaaagacg tgggtattat cgccactgaa
    25321 aaaggctgga acctgtatgt ttgcggtaac ggcggcatga aaccgcgtca tgcggatctg
    25381 ctggcagcgg atatcgatcg cgaaacgctg atcaaatatc tcgaccgctt catgatgttc
    25441 tacatccgta ccgccgacaa actgacgcgt accgcgccgt ggttagaaaa cctcgaaggc
    25501 ggcatcgatt acctgaaagc ggtgatcatt gacgacaaac tggggctgaa cgctcatctg
    25561 gaagaagaga tggcgcgcct gcgtgaagcg gtagtgtgtg agtggactga aacggtcaat
    25621 acaccgtccg cgcagactcg cttcaaacac ttcatcaaca gcgacaagcg tgacccgaac
    25681 gtgcagatgg tgccagagcg cgaacagcac cgtccggcaa cgccgtatga acgtatcccg
    25741 gtaactctgg tggaggacaa cgcatgagcc agtggaaaga catctgcaaa atcgatgaca
    25801 tcctgcctga aaccggcgtc tgcgcactgt taggtgacaa gcaggtagcg attttccgcc
    25861 cgtatcacag cgaccaggtg tttgcgatca gcaacattga tccgttcttc gagtccagcg
    25921 tgctatcacg cgggctgatt gcggaacacc agggcgagct gtgggtcgcc agcccgctga
    25981 aaaaacagcg ttttcgctta agtgatggct tgtgcatgga agacgaacag ttttccgtca
    26041 aacattacga agcacgagtg aaagacggcg tggtgcagct gcgcggttaa tgttttaacg
    26101 ggaggcgcaa tgcctcccct ttttgcatgg ccctgtaatg accttcggta tattgcagga
    26161 cattttttaa actttttgtt ttattttttg tttttatttt ttaaaggata atcaaatgtt
    26221 tacagacact attaataagt gtgcggctaa tgctgcgcgc attgcacgcc tgtcggcaaa
    26281 taacccgctc ggcttttggg tcagctccgc catggcgggc gcatatgtgg gtcttgggat
    26341 catcctgatt ttcacgctcg gtaatttgct cgacccgtcc gtacgtccgc tggtgatggg
    26401 cgcgaccttt ggtatcgcct taacgctggt gattatcgcc ggttctgaac tgttcaccgg
    26461 acacaccatg ttcctcacct ttggggtaaa agcgggcagc atcagccacg ggcaaatgtg
    26521 ggcaatcctg ccgcaaacct ggctgggtaa cctggtcggt tccgtcttcg ttgccatgct
    26581 ctatagctgg ggcggcggta gcctgctgcc ggtagatacc agcatcgttc actccgtcgc
    26641 gctggctaaa accactgcac cggcaatggt actgttcttc aaaggtgcat tgtgtaactg
    26701 gctggtttgc ctggcaatct ggatggcgct gcgcactgaa ggggcggcga aatttatcgc
    26761 tatctggtgg tgtctgctgg catttatcgc gtccggctac gagcactcta tcgctaacat
    26821 gacgctgttc gcgctctcct ggttcggcaa ccacagcgaa gcctacacgc tggcgggtat
    26881 tggtcataac ctgctgtggg tgacgctggg taatacttta tcaggtgccg tattcatggg
    26941 attgggttat tggtatgcta cgccgaaagc gaatcgtccg gttgcggaca aatttaatca
    27001 aactgaaaca gctgccggtt aattactaag gggtttttac gtggatcatt tgcctatatt
    27061 ttgccaatta cgcgatcgcg actgtctgat tgtcggcggt ggtgatgtcg cggaacgcaa
    27121 agcaaggttg ctgttagacg caggcgctcg cttaacggtg aatgcattag cgtttattcc
    27181 acagttcacc gcatgggcag atgcaggcat gttaaccctc gtcgaagggc catttgatga
    27241 aagccttctc gacacctgct ggctggcgat tgcagcgacg gatgatgaca cgcttaacca
    27301 gcgcgtcagc gaagccgctg aagctcgtcg catcttctgt aacgtagttg atgcgccgaa
    27361 agccgccagc tttattatgc cgtcgattat tgaccgctca ccgctaatgg tagcggtctc
    27421 ctctggcggc acctctccgg ttctggcgcg cctgttgcgc gaaaaactcg aatcactgct
    27481 gccgttgcat ctgggccagg tggcgaaata tgccgggcaa ttacgcgggc gagtgaaaca
    27541 acagttcgcc acgatgagtg agcgtcgccg tttctgggag aaattgttcg ttaacgaccg
    27601 cctggcgcag tcgctggcaa acaacgatca gaaagccatt actgaaacga ccgaacagtt
    27661 aatcaacgaa ccgctcgacc atcgcggtga agtggtgctg gttggtgcag gtccgggcga
    27721 tgccgggctg ctgacgctga aaggactgca acaaattcag caggcagatg tggtggtcta
    27781 cgaccgtctg gtttctgacg atattatgaa tctggtacgc cgcgatgctg atcgcgtttt
    27841 cgtcggcaaa cgcgcgggat accactgtgt accgcaggaa gagattaacc agatcctgct
    27901 gcgggaagcg caaaaaggca aacgcgtggt gcggctgaaa ggtggcgatc cgtttatttt
    27961 tggccgtggt ggcgaagagc tggaaacact gtgcaacgcg ggtattccgt tctcggtggt
    28021 tccgggtatt accgcagctt ctggttgctc tgcctattcg ggtattccac tcacgcatcg
    28081 cgattatgcc cagagcgtac gcttaattac cggacactta aaaaccggtg gcgagctgga
    28141 ctgggaaaac ctggcggcag aaaaacagac gctggtgttc tatatggggt tgaatcaggc
    28201 cgcgactatt cagcaaaagc tgattgaaca cggtatgcct ggcgaaatgc cggtggcaat
    28261 tgtcgaaaac ggaacggcag tcacgcagcg cgtgattgac ggtacgctca cgcaactcgg
    28321 tgaacttgct cagcaaatga acagtccatc gctaattatt attggtcggg ttgttggcct
    28381 gcgcgataaa ctgaactggt tctccaatca ttaatttaac ccgggccaga gaattctggc
    28441 cttcttaatg actcttttta tatattcagc aaataaaaca taatccctta ataaatatta
    28501 tctgctaatg ggttatattc accgttacag cgatttacaa aattaagtca ttcgctcact
    28561 gacgccaatt aaatattcct gtatcgttct taacgagcag tctgcttata ttcatataat
    28621 caatgaatat taattaatat ataatacata gggaatgtaa atgaacaaat ttattaaagt
    28681 tgcactggta ggtgcagtac tggctacgtt aactgcatgt actggtcata ttgaaaaccg
    28741 tgataagaac tgctcttacg actacctgct gcacccggca atttctattt ctaaaatcat
    28801 tggcggttgc ggtcctactg cacagtaagt ctctgacaga aatcggctaa caccggttta
    28861 attgcccgac gccctctgga ttttccgagg gcgtatttgt ttctatgtcc ttcttgcccc
    28921 cgcccgtaaa aatattttca ctttaaattc aattttataa ctacatccat tagccacagc
    28981 gcgatcctgt gcgaaatttt gtgatcttcc tccacatcac ataacataat atgttgttat
    29041 attcatcatg cattgtcatg ttacctttta aatggctgca aactctcccc tacaacgtat
    29101 tggacaagaa aaaggtatcg ctatgggaag ccaggaactc caacgcaagc tcggattttg
    29161 ggccgttctt gcaatcgccg tcgggacaac cgtcggctcc ggtatttttg tatctgtggg
    29221 tgaagtggca aaagcagcgg gcacgccgtg gcttacggtg ctcgcgtttg tcattggcgg
    29281 gttaattgtg atcccgcaaa tgtgcgtcta tgcggaacta tccaccgctt atccggaaaa
    29341 tggcgcagat tatgtttatc tgaaaaatgc cggaagccga ccgctggctt tcctctccgg
    29401 ctgggccagc ttctgggcca acgatgcgcc gtcattgtcg attatggcgc tggcgattgt
    29461 cagcaatctt ggctttttaa cgcctatcga tccgttgctc ggtaaattta tcgccgccgg
    29521 attaattatc gcctttatgt tgctacacct gcgctccgtt gaaggcggcg cagcgtttca
    29581 gacgctaatt accatcgcca aaattatccc gttcactatc gtcattggcc ttgggatctt
    29641 ctggtttaaa gcggagaatt ttgccgccct taccaccact gcgattggcg caacgggcag
    29701 ctttatggcg ctgctggcgg ggatctctgc caccagttgg tcgtataccg gcatggcctc
    29761 tatctgttat atgaccggcg aaattaaaaa ccccggaaaa accatgccac gagcgctgat
    29821 tggttcctgt ctgctggttc tggtgctcta caccctgctg gcgctggtga tttccggcct
    29881 gatgcccttc gacaaactcg ccaattctga aacgccgatt tccgacgccc tgacctagat
    29941 ccccgcactc ggcagcaccg ctgggctctt tgttgccatc acggcgatga tcgtcattct
    30001 tggttcgctt tccagctgcg tgatgtacca gccgcggctg gaatacgcga tggcgaaaga
    30061 caacctgttc tttaaatgct tcggccatgt gcatccgaaa tacaacacgc cggatgtctc
    30121 catcatcctg caaggggcgc tggggatctt cttcatcttc gtttccgatc tcaccagcct
    30181 gctgggttat ttcaccctgg tgatgtgttt caaaaatacc ctcaccttcg gctccatcat
    30241 ctggtgtcgt aaacgcgacg attacaaacc gctgtggcgt actccggctt tcgggctgat
    30301 gaccaccctc gccattgcgt caagcctcat tctggtcgcc tcaacctttg tctgggcacc
    30361 gattcccggc cttatctgcg ccgccatcgt tattgctact ggtctgcctg cttacgcctt
    30421 ctgggcgaag cgtagccgcc agctcaacgc tttgtcgtaa tgttatctga agaaaataaa
    30481 atgttggata ttgataaaag caccgtggac tttctggtca ccgaaaatat ggttcaggaa
    30541 gtggaaaaag ttctcagcca tgacgttccg ctggtgcacg ccatcgtgga agagatggtg
    30601 aagcgcgaca ttgatcgtat ttatttcgtt gcctgcggct cgccactcaa cgcggcgcaa
    30661 acggcgaaac atctggcgga tcgcttttcc gatcttcagg tctacgccat ttccggctgg
    30721 gagttctgcg ataacacccc gtatcgcctc gacgatcgtt gcgcggtgat tggcgtttct
    30781 gactacggta aaacggaaga ggtaatcaaa gcgctggagc tgggccgggc ctgcggagca
    30841 ctcactgcgg cgttcaccaa acgcgcggac agcccgatta cctcggcggc ggaatttagc
    30901 attgattatc aggccgactg tatctgggaa attcacctgc tgctctgcta cagcgtggtg
    30961 ctggagatga tcacccgcct cgcgccgaac gcggaaatcg gcaagatcaa aaacgatctc
    31021 aagcagttgc cgaatgcgct cggtcatctg gtacgcacct gggaagaaaa aggccgccag
    31081 cttggtgaac tggccagcca gtggccgatg atttataccg ttgctgcggg tccgctgcgt
    31141 ccgctgggtt acaaagaagg cattgtaacg ctgatggaat ttacctggac gcacggctgc
    31201 gtgattgaga gcggagagtt ccgccatggc ccgctggaga ttgtcgaacc gggcgttccg
    31261 ttcctgttcc tgctcggcaa tgatgaaagt cgccacacca ccgaacgcgc cattaacttt
    31321 gttaaacagc gtactgacaa cgtgatcgtc atcgattacg ccgaaatttc gcaagggctg
    31381 cacccgtggc tggcaccgtt cctgatgttc gtgccaatgg agtggctctg ctactacctg
    31441 tctatttaca aagatcacaa cccggatgaa cgccgctatt acggtggtct ggtggaatat
    31501 taatccctct cccggcccgg caacgggccg gatttacgca aggagttacc cgatgaaaac
    31561 aggtatgttt acctgcggcc accagcggct gcctattgaa cacgcatttc gtgatgcaag
    31621 cgagctgggt tacgacggca tcgaaatttg gggcggtcgc ccgcacgcgt tcgcgccgga
    31681 cttaaaagcg ggcggcatca aacaaatcaa ggcgctggcg cagacgtatc agatgccgat
    31741 tatcggttac acgccagaaa ccaacggcta tccgtataac atgatgctgg gcgatgaaca
    31801 tatgcgtcgt gaaagcctgg acatgatcaa gctggcgata gatatggcaa aagagatgaa
    31861 cgcgggttat acgctgattt ctgcggccca cgcgggctat ctcacgccgc ctaacgttat
    31921 ctgggggcgg ctggcagaga acttaagcga actgtgtgag tacgcggaaa acatcggcat
    31981 ggatctgatc ctcgaaccgt taacgccgta tgaatcgaac gtcgtgtgta atgctaatga
    32041 tgtgcttcat gcgctggcgc tggtgccttc gccgcgcctg ttcagcatgg tcgacatttg
    32101 cgcgccgtat gtccaggcgg aaccggtgat gagttatttc gacaaactgg gcgataaatt
    32161 acgtcatctg catattgtcg acagcgacgg ggccagcgac acgcattaca ttcctggaga
    32221 aggcaaaatg ccgctgcggg aactgatgcg cgatattatt gatcggggct atgagggtta
    32281 ctctacggtg gagctggtga cgatgtatat gaacgagccc agactctatg cccgccaggc
    32341 gctggaacgc tctcgcgcgc tgctgccgga ggatgagaga tgaaaaccct ggcgacaatc
    32401 ggcgataact gcgtcgatat ctacccgcaa ctgaataaag cgttttctgg cggtaatgcg
    32461 gtcaatgtgg cggtgtactg cactcgctac ggcatacagc cgggatgcat tacctgggtg
    32521 ggtgacgatg actacggcac aaagctgaag caggatctcg cccgcatggg cgtcgatatc
    32581 agccatgtcc atacgaaaca cggcgttacc gcacaaactc aggtggaact gcacgacaat
    32641 gatcgcgttt ttggcgacta caccgaaggc gtgatggccg actttgccct gagtgaagag
    32701 gattacgcct ggctggcgca gtatgacatt gtgcacgcgg caatctgggg acatgcggaa
    32761 gacgcattcc cacagctgca cgctgcgggc aaacttaccg ctttcgactt ctccgacaag
    32821 tgggacagcc cgctctggca gacactggtg ccgcatctcg attttgcctt tgcctccgca
    32881 ccgcaagaag acgaagcgct gcgtctgaag atgaaagcga ttgttgcccg tggcgcagga
    32941 acagtgattg tcacgctggg tgaaaacggc agcattgcct gggatggcgc gcagttctgg
    33001 cgtcaggctc ctgaaccggt gacggttatc gacaccatgg gtgccggaga ttcgttcatt
    33061 gccggattcc tttgcggctg gtctgcgggg atgacattac cgcaggcgat ggcgcaggga
    33121 acggcgtgcg cggcgaaaac cattcagtac cacggtgcct ggtaggtata acgttggcgt
    33181 gagcatcttc acgccaacgt gctgttactt gccggaaaac gaccctataa tccgagtaat
    33241 tcattcttta tttcagggtc gattatgtca gctacggacc gctactctca tcaactcctc
    33301 tacgctaccg tccgccagcg gctgctggat gatatcgcgc agggggttta ccaggccggg
    33361 caacagatcc ctaccgaaaa cgagctttgt acacaatata acgtcagccg cattaccatt
    33421 cgcaaagcca tcagcgactt agtggcagac ggcgtactga tccgctggca gggaaaaggc
    33481 acctttgtac aaagccagaa agttgaaaac gccctgctta ctgtcagtgg ttttaccgat
    33541 tttggcgtct cacaaggcaa gtcgacgaaa gagaaagtga tcgaacagga acgggtcagc
    33601 gccgcgccgt tttgcgaaaa gctgaacatc cccggaaaca gcgaagtgtt ccatctctgc
    33661 cgggtgatgt atctcgataa agagccgctg tttattgata gttcatggat cccgctgtcg
    33721 cgttatcctg actttgatga gatttacgtc gaaggaagct ccacctatca gttatttcag
    33781 gagcgttttg acacgcgagt ggtcagcgac aaaaagacca tcgatatctt tgccgccacc
    33841 cgcccgcagg caaaatggct gaaatgcgaa ctgggcgaac cgttgtttcg catcagcaaa
    33901 atcgcctttg atcagaatga caaaccggtg cacgtctccg aactcttctg ccgcgccaat
    33961 cgcatcacct taacgattga taataaaaga cattaaccgt aggccggata agatgcgcca
    34021 gcatcgcatc cggcgatgct ggcgcactga attttacatc ccgcacgttc ccctcaccct
    34081 aaccctctcc ccaaaggggc gaggggaccg ttcagtgcgg aatttagctt gattgccgaa
    34141 cggtgctatc cagcattaaa tactcgcaat actctcacgc aaaatccgca gcaccgtctc
    34201 ttcaccgctg cgattcgggt taatgcgaat cgcacaatgt tcgagttgtg gattcgcctg
    34261 gcgaaacgtt cctgaaagtc gatagaagag cggcgggatt tcatatttcg actctgcacc
    34321 gaccgggtaa ggcaacgcgc cacgcttttg cgcttcttcc agcaccctgg cggcaatcag
    34381 ctgatgaaac tcgacaatca acaccttcga ctgcgcattg gcaatgacgg cgcttttcac
    34441 ttccgccacc gcgccgccgt taagcaaagc cagcaaccgt tcggatactc ccgcctgcac
    34501 cgcatgcatc actggcgcga acaccagacc acgcaagact tccagcgcct gtgagccctg
    34561 gatctggcta ccgccggagt aaagcgtggc gcgaatccga ctaataacat cggcatcgcc
    34621 aaccactgca ccaacgccct ccggcccaaa cagcttgaag caggagaatg tcgagacatt
    34681 cgcgccgcat tcacagccga ttcgcgctac cttcatcacc gcatagttat catcggttaa
    34741 cgctggaaca cctgccgcgc gcaacgttgc cagcacatct gccagcacat agctgtcctg
    34801 cggctgctgg cgcgtatgct gcaccagcgc ggcatcaggt tgttgctcgt cgacgacctg
    34861 cttcagtgcc gacaggtcat tgaaatcagc agtaataagc gtcagcccca tctgctcaat
    34921 aataacccgt gtcgtcgggt aaacaggcgc gtcatgcacc agaagacgct gccccggttt
    34981 gagcaaagcc gccagcccgg cgcgaatcgc gccagtcccc gcgccctgca ccagcgccgc
    35041 ggcctgtgcg tgaaatgcat cagccagcac ctgctccacc cgttgggtaa tacgcggttg
    35101 attcagactt ggagttaacc ctaaatcacc gccagcaaga aactcgctgc ctggaaaatg
    35161 gcggcaaata gtgtccacca gcgcaaactg cttttgctgc gcttcagcaa gagtcaggct
    35221 ttgcaaagga aacgtcttca taggtgcctt ccttaagccg gaacgaacag gccaagccag
    35281 taaagaacat tcagcagaat accggtgatc attactgcca cgaccggtgc cgccattttc
    35341 tgtaccggac ggcccaggga ttcgttgagg aagtaaatgg caaccgcgat agagaatccg
    35401 gtataacccg ccatcttaat tgctgcgaaa atcgaaccga ccagcagcgc cacttccatc
    35461 agcatattca tggcgttacg gatgttatcc gacgcattac gcaccgacgg gtagcgtccc
    35521 agccatttgc cgatcgaacg aagcagcaag acttccgccg aaataaccac tgcgcctaat
    35581 accgctgcaa ccatcggatt cggcgagaga tagcccaccg cataaacaaa ggtaaagccc
    35641 gcaactgcat acacacccgt tgctaaggcg gtggtggcaa tcaacggcac aaaccccagt
    35701 ccgcgcataa attctgccag agccgcctga ttaatcagcg tttgcgattg ttccggcgtc
    35761 acgcctgcgg aatacgcttt ctccagtgtg aagatcgaaa cttcgctacc agcaaaaatc
    35821 ttcatactgg caacggcggc aatcaatgct cccacgatgg cgatataagg taagtttttg
    35881 ataatccgcg acgtgcgctc ttcaaacacc gaaagcccgc tggcatcgat gtcattttca
    35941 tcacgatgac gcaggtcgtg agttatcgcg atccccagca gcatcaccat gccaataaag
    36001 atttcgatgg attcagggtt aagatgtggg aaatagcgca cgactaccac acgggtcatc
    36061 agaacaacca cggcggcgac cagactttgc ttccagccaa actggtagaa aatcgccacc
    36121 agcgggaaca gtgcaaaagc agaaaccacc ggcgagctta attccccgag gctacctaat
    36181 acatccaccg gcagcgcggt cagcagctgg ttaactggca acaggcaagt aaggatcaac
    36241 acgccccaga tagcgccaag accaaacgcc atcaggctgt ttatcgccag tacgccgagg
    36301 atatcggtag gaagaaagag taaccatgcg ttgagcagcc cggttttcag ggtaaaagag
    36361 atccccaccg aggccacaaa accaatgctc agaccaaaag cgatactccc cgcctcgcga
    36421 cggttcatat agccttcaat cagttgcggc aggatcgggt ggatgccgtc atgaaaaaca
    36481 gccgccgagc gatgcgccag aagcgatgtc atacccgtca ggcacgccac cacgataatc
    36541 tgaatataca gatccatagt cgtgcctttt atttcagatg gttaatcaac atcggaatcg
    36601 cgtgttcgac gtgctcaacg gaaaggccaa acgccacttt cccttcagcg atcattttgg
    36661 cgatatgctc gtctttcgct ttaatgcctg gtttggcaat ggtgcaactt ttgttatagc
    36721 caatcaccgc gatggcaatc gacaatgccg cgcccgcacc ggtattacac gcgccaatgt
    36781 aataatctag ttgcccggat ttcaccttca ttgccgcttc catgtcgttg tgaatgaaaa
    36841 cttcaaaaca gccaggagcc gtcgcttcaa tggttttttt aatctgctca cgctgtaagc
    36901 ctgcaacgcc aatctttttc atgagtctgt ccttattgga aaaattgaga gggattttca
    36961 cgtaacatca catccacatc ggcctgactg aatcctgact ggcgcaattg cggaataaag
    37021 gtggtcaata aatagtcata gccataacca ccattggctt ttaaatggga gcggcgcgta
    37081 atatccatcg acagcatgac gcggttcagc aacccacggt cacgtagcgc gtggagcatc
    37141 gcaatgcgct tttcgtccgg gtagtaactg ttcttaccaa tggtgtcgaa ctgcacgtac
    37201 gcgccgagat cgatcatctt caaaatgttg tcgaggttgt ctttcagatc gcagtgacca
    37261 acggtgacgc gcgaaagatc aactccgtgg gcttgcaaca gagccagttg ttccagcccc
    37321 atggtgctga acgacgtatg cgtggagatc gggcgtccgg tctggttatg cgccagcgca
    37381 gcggcaataa ataccttctc ttccagcggc gtaatctttc cttcgctgga gccgatctcc
    37441 gcgatgatcc cggctttcag ctccgtgcca tcgataccct gttcaatttc atcgaccatc
    37501 tcctgcgcca gttcctgcac gctgcgggtc accacatgtt ccgggaaaaa cgcatcctgg
    37561 taataaccag tacaggccac cacgttgatc ccggtttcgc gcattacatc gagcataaat
    37621 tgcgcattgc gccccatgta acggttggtc atctcaatca cattacgtac accccgggtc
    37681 atcaggtcgt tcatctcctg gcaaatgaac gcatactgat caaggcggca gtccacgttg
    37741 tttttaaagc cggagagatc aatatgcaga tgctcatggg ccagggtgta acccgtcgga
    37801 tcaaaactca tagggtgtct cctgcaaacc ggagggagga aagaaaagag cgaccatttt
    37861 gtggcggtgg cgcacggaaa aattcacaca cggtagctcc cacatcagaa agcgtggtgc
    37921 gcacaccgag ctgcgtggcg accagccctt gctgataaac cagcactggc accacttcac
    37981 gggtatggtg gctgtgacca atggtcggat cgttaccgtg atccgccatc acgaccaggc
    38041 aatcatctgg ctgcatcgcc tcaacaagcc gggcaaggtt acggtcaacg acctgcaaac
    38101 gttcggcata acgtgcgacg tcttctgcat gaccagcgag gtcggtttcc tggatattga
    38161 tgcaaataaa cgccgtcgga tgggtgttaa attcgtcgag ggtgatatcc ataatccgct
    38221 ggctatccac caggttttgc cagctcacac cataaggatt gctgacgata tctgccacct
    38281 tacccaccag cacggttggc acgcccgctt catacagttt ttgcggcacc tgcacttttt
    38341 catcaacacc gtagcccata tgcacgacct ggaagccgtt gtcataagcg ccagaacgcg
    38401 gcgcattaat accaataaag cgcccttctt tgctttctgc ggcatcgaga atacgctgac
    38461 tgttggttaa caggccgcca aatgtaatga cccgaccgac ctgtacctgg tcacgcacga
    38521 tacgaccaat tttgattgcg tcgtcaaaag agatcacaga gagattggcg gtaatgttat
    38581 agacttggcc taaatccgcc tcgagattat cgccaatcgc aaccgcctga ttgaccgaca
    38641 gaaattgcag atcatcgcca cggcgctcca cctgccagcc agcggaaact aatgcctgct
    38701 caacacggtc aatcacatcg cgaaaaggca tccgcagcgg cggtaacggg cgcgtgccta
    38761 aaatttcctg atgccccata aaggtatcgc caccttcatg ttgcagctct gccacgcccc
    38821 aggttgcgga atctgacggc tgcatatcgc ctggcgcata acccaatgtg ttgattagcc
    38881 ccagcttctc cagcgttggt agctgcaaat gcggcaactg gctcaggatg tgaccacagg
    38941 tattcgcccc ggcatcttgc gggcgcacca gcgtgacatc tttcattgcc cctacgccaa
    39001 agctatcaat cactaacacc acaaatcgcg ccatttatgc ctccagaggg ttgccgaggc
    39061 tgtcataacg accaactatt tctggttcgc cgcgctgaat accggacacc agcaccacat
    39121 cgctacgggt gacaaaaatc tgcgtgcgaa aacagagcac cactgcactg ctcaccggat
    39181 actcgcctgc cagcggcagg gtgtagtcga tactgctgtc atccacagtt ttgagattgg
    39241 tttcagtaat cttttgattt tctggcgtaa acaccagcgc atgttgcgca tgaccacgac
    39301 ggtagtaacc gccgccgtag cagtagctgt cgccacggaa atgatgggag atttcactta
    39361 accagagcat cgcgatacgt tcaggctgat cgccctgctg gtttgccgga atagtaccgg
    39421 tcagcgcatg accgggttcg gcatgagtcg caccgtattc cgccagcaat ggcagcgaag
    39481 tgcagctggt cgctgaaggt acgttcagtt gctcaatcgc aataccagat ttcgccagtt
    39541 gatcccgtgc ttgtaccagc gtgtgaagat tcggtgttgg caaaactttc ccagcagcct
    39601 catcccaaag caggcaaggg aaatgggtta gtccggctaa atgcagcccc ggcagtttct
    39661 taatttcagc aatcacctcg tgcagcgaat gctgtacaaa accgctctcc tgccccggat
    39721 aaagaaaatc atcgtcgcta taaactttga gcagcacagg ctggacccgt ccggccttaa
    39781 ccgctgccgc agaaacttcc cgcgctttgt cgagagtaaa cacggtgatg atgtcggtgc
    39841 cctgttcaac ggcgtcagca acctgatgac aagggatttg taccagatgc ccctgatgcg
    39901 ccactggcaa accagcgcgg cgcatgactc gcgcctcttt gtaatccacc gccacaatgc
    39961 cgctgtagcc taatgccagc aatttttccg ccagccacgg attacgacca aattgcttgg
    40021 tcatcagata cagttcaatg ccgtaaagcc gcgccgtctc aatgagccgc ttaccgtttt
    40081 ccagtatctg atccacgtcg atcacccagc tgtccggggc gattttgccc tgctgccaca
    40141 aacttagtgc ggcggaaatc agcgccgggt tctggcgttt caatgcttct acaaacatct
    40201 tgttgcctcg tttctgttcc ttgaattaaa tattcatttt tttgaatatt taggttgtgc
    40261 gacgtggatt tgtcgggagc ttcaaacctc ttcgttagca gccatccata atccataaag
    40321 attggccaga aaatagcctt cttcacacgg gttaacttcc agagcgaatt ccttcaacaa
    40381 ctcctgatgc aattgcacaa cggcttgcca gtggctggat tgcgccagtt ccgccagcaa
    40441 ctcgttatcc agcggctcta tttcttcacc acggcgactg cgcatcagtg cactcgccat
    40501 atgtgtcatc gccatcgttc cttgctcact gcgcaccggc agatggcact ctttttccag
    40561 tacgttgacg acctgcatca tgcctttgca gacgtcctta tcaataacgc ctgcctcgca
    40621 aagcaggttg agtctggttt ccatatcaga tgcctttcgt ttaataactg ggttcctgtt
    40681 ccccactcac cacccgttgt tgatgggcaa gcagggcgtg tttatctaca acagcacgca
    40741 gcaattgttg cattgggtaa tcatcgactc gcgtcaggac cacggcttcg gtggcctgta
    40801 aaaatcgcgg atcgtctgtt agcggcgtcg cctctaatcc cagcatggtc agttcgtttt
    40861 ccgccaccac gttccagatc accgcatcga catcgccttt aacaatgcgt tgtaaactct
    40921 cgtgataaga gagatcgact cgttccacat cactatcgcc aaaaaagaca tcggtcatga
    40981 ttttctgatc cgccgaacga ctatcgagac ctactcgctt aacatttcca gactcacctt
    41041 tgcggcatat cagttgatgt tcaccaacat acgtatgcgg gccaagctcg agggcgatgc
    41101 acaaattgtt ttggctgaga taactttccg ccgccaggcg ggaaactact gccatgtcat
    41161 agacaccgtt aagtaaacac tccacgcgaa tatccgcgcc gcgcatatgc gcatagtaaa
    41221 aaggaatgcc atcaaactgg gctttcaatc cgctcgctaa accttcgtac aagcgggtat
    41281 agggcaaagg catcgcacat accacgttgt tgatatccac atgactcagc aatgctttgt
    41341 tatccatctc gacaagataa ctgccattgc gcccacggcg ttcaatccgt atcgctccgc
    41401 ttgattccag cgttttcaac gcggcctgcg tcaggccaac cgatgaacgg cattcagttg
    41461 ccagctcatc tatggttttc agtcgattac cgcatttttc acccaacaaa taacgggcca
    41521 gtgtcgtgat gacgacgcct tcttttttga taaacgttcg acgcataata tattttcaat
    41581 aaaatgaata tttatatctt cattaaaatg aagataagtg gcgacgacga gatgtggaaa
    41641 cggcgaggcg cttcacattt tttcgggttg ttgcgaaaag aggataaaaa aaccgggttt
    41701 ccccggtttc agagtgatga cgaagtgatt tattcgacaa tgcgagcaaa gggtaattcc
    41761 cactggcgct tgcctgctcc cctcacccta accctctccc caaaggggcg aggggaccgt
    41821 ccgagcgttt tgatattacg gcttcgccac aaaaccaatc gcttcgtaca ccgctttcag
    41881 cgtacgggaa gcgtgtacac tggctttttc tgcgccgtct ttcatcacct gttgcaggaa
    41941 ggcttcatcg ttgcggaaac ggtgatagcg ttcctgcaat tcagtcagca taccggaaac
    42001 ggcatcagcc acttcacctt tcagatgacc atacatcttg ccttcgaact gtttttccag
    42061 ttctgggatg ctctggcccg ttaccgcaga aaggatatcc agcaggttgg aaacgcccgc
    42121 tttgttctgc acatcgtagc gaactaccgg cggctcgtcg gagtcagtga ccgcgcgttt
    42181 gattttcttc actaccgatt tcggatcttc cagcaggccg ataacgttat tgcggttatc
    42241 gtcagacttg gacatcttct tggtcggctc cagcagcgac attacgcgcg cgccagattt
    42301 cggaataaac ggctccggca ccttaaagat ctcgccatac agcgcgttga aacgctgggc
    42361 gatatcgcga ctcagttcca ggtgctgttt ctggtcttca cccaccggta ccagattagt
    42421 ttgatacagc aggatgtccg cagccatcag caccggatag ccaaacagac cagcgttgat
    42481 gttctcggca taacgcgcag atttatcttt aaactgcgtc atgcgactca gttcgccgaa
    42541 gtaggtatag cagttcagtg cccagcctaa ctgcgcatgt tctggtacgt gggactgaac
    42601 aaaaatggtg cttttctcag gatcgatacc acaagccaga tacaaggcca gcgtatccaa
    42661 cgtcgcttta cgcagcttct gtgcatcctg gcgcacggtg atcgcgtgtt ggtcaacgat
    42721 acagtaaatg caatggtagt catcctgcat gtttacccac tgacgcagcg cacccatgta
    42781 gttaccaatg gtcaattcac ctgagggctg tgcgccacta aaaacgatgg gcttagtcat
    42841 ttttcgattc ctgattttcg ctatgcggaa gccctaatgc gggcagaagg tcatttatag
    42901 actgataaat tacatcaggc tggctgagat cgatagcctc gccgtagttg tatccgtagg
    42961 ttaagccaac tgatgggcaa cctgccgctt ttgccgcctg aatatcattc cgtgagtcgc
    43021 cgacaaacag catctgttgt ggggcaattc ccatccgctc agccaccagt aacagcgggt
    43081 ccggatgcgg ttttttgttt tgcacatcat cgccgccgat aaccacgcta aagtatttgg
    43141 caatatctaa ggcttcgagc agcggcgcga cgaacggcgt cggtttgttg gtgaccaagc
    43201 ctagcggcag gcctttagcc tgcaacgcgc ccaacgtatc ggcaacgtgc gggaacaaaa
    43261 acgtcccctc ttcggcaacc tcgccatagt agcgatcgaa cagtttacgc agaatacgta
    43321 cctgttcttc tgccggaatg tcgtcatcaa cgggcggttt ccccattgtt ttacgcagag
    43381 tcgcacgttc ctgacgcgcc caggtcaatg cgcgctccat cagaacatct gcgccgttac
    43441 caatccaggt aataacgcgt tcttcacctg cgacgggcaa ctccagcgca tacagcgcca
    43501 tatctaccgc agcagcaaga ccaggagcac tgtcgaccag cgtaccatca agatcaaaag
    43561 cgacgccgcg aatatcttca aacttattca tgacttacct ttgccagttc actgcgcatt
    43621 tcatcaatga cttttttata gtctggctgg tcgaagattg ccgaaccggc gacgaacata
    43681 tccgcgcccg ccgcagcgat ttcgccaatg ttgttcacct tcacgccacc gtccacttcc
    43741 agacgaatgt caaagccaga ctcgtcgata cggcgacgta cttcgcgcag tttatccagt
    43801 gtttgaggaa tgaaagactg accgccgaaa ccagggttga cggacatcag caggatcaca
    43861 tccagcttat ccatcacgta atccagatag ctcagaggtg tcgccgggtt aaataccaga
    43921 cccgctttac agccattttc tttaatcagt tgcagcgtgc ggtcaacatg ctcggaggct
    43981 tctggatgaa aggtaatgat gctggcacca gcggcagcga aatcaggcac aatgcgatcg
    44041 acgggtttca ccatcagatg tacgtcgata ggggcggtaa tgccgtagtt acgcaaggat
    44101 ttcagcacca ttggcccaat cgtcagattg ggaacatagt ggttatccat gacgtcaaaa
    44161 tgcacgacat cagcgccagc tgccagggct tttgcggtat cttcacccag gcgggcaaaa
    44221 tcagccgaca gaattgaggg ggcaatcaaa tactgtttca tccgcttctc cttgagaatt
    44281 attttttcgc gggtgaaacg actcctggtt tgtacaaagc cagcagttcg tccacctttt
    44341 tacgtgtgcc gccgttgctg cttatactgc gtcgaacttt gacgacatgc aattttgcgc
    44401 gctgatacca ctcacgcgtt aacatcgtat cgtgattgga gatcagcact ggaatatggc
    44461 gctcaaccag accttcggcg atctccgcca gatgcgcttg ttgttcaagc gtaaaactgt
    44521 ttgtgtgata cgccgtaaag ttggcggtcg cagacagcgg tgcataaggc ggatcgcaat
    44581 agacgacgga tgcatcatct gcgcgctcca tgctatcggc gtaagactca caatagaaaa
    44641 aggcattctg cgctttttca gcgaagtgat acaactctgc ttccgggaaa tagggttttt
    44701 tgtagcggcc aaacggcacg ttaaactcac cgcgcagatt gtaacgacac aggccgttgt
    44761 aaccgtagcg gttcaaatat aaaaacagta ccgcccgacg gaacggatcc tggcttttgt
    44821 tgaactcttc gcggaactga tagtaaacct cggcgcaatt tgtttcggga acaaacagct
    44881 cgcgtgcggc ctgtacgtac tcatcagtac gcatcttcac aatgttatag agactgatca
    44941 ggtcgctatt gatatcggca aggatataac gagaaaagtc ggtgttgaga aacaccgacc
    45001 cggcacctac aaaaggctca accagacatt cgcccttggg caaatgccgt ttaatatcat
    45061 caagcagggg atacttgccc cctgcccact tcaaaaaagc gcgatttttc ttcatgccga
    45121 ttaactaatt acaccttctc cggctgtgga gaaagctccg acagcatcca gcgcttcagg
    45181 cagctcccgc aacgataaac cattgcggga gataaccttg attacttcag atcggcctgt
    45241 acctgacgca gcggtttcgc ccacgggttt ttggcctgaa catctgctgg caatgtagat
    45301 accgcttttt tcgcctcttc tttcgaagcg tacacgccag aaaccaggac ataccacggc
    45361 tgaccattac gcgtcgtttc atagacaacg tagtttttca gattctcttt cttcgcccaa
    45421 ccgttcaggt tgtcgtagtt agaggaactg ctcagctgca gagtgtaatg gctggacggt
    45481 gccgatttca acgaaccaac attacctgcg gtcttccctc cagcggctgg tgttgccgtg
    45541 gtttgcgccg gggatgccgt ctgtactgga gccgtagtcg ccgtctcttt tggtgctggc
    45601 gtagaagtcg cagccggtgc cttcgtgctc gccacagggg cagctggttc aggttcagta
    45661 cgcttcggcg tctgtgctac cggcttcggc tccgttttca cggttgcttg cggttttttc
    45721 ggttcaatca ccgcctgctg acgaactggg cgcgtagtgg acggacgttc agcggtttgc
    45781 gttttcgccg tgtcacgcga tgcattgcca ttgcgaacag gcgcaaccgt tgcgggttca
    45841 gtcggcaacg tggaattgac cgccacattg ttcagctgtt gctgattttg tggctgggtc
    45901 agcgcattgt tcaggtcacc ctgcacttca acacgttgtt gaccatccgt tgccaccggg
    45961 gtttgccctt gagtcggcgt agaagagatc ggtggcagag aaacatcctg ctgagtattt
    46021 tccgcagacg tggttcccgg cgctggctgc acgccattcg cctgatcggt cgcattgcca
    46081 gcaagatcaa tactcttctc gccagacgcg gtttgatcgc tggaagtggt cgagggggct
    46141 tttagcgcag aaccgatacc gatgatcaac agcagtagaa ccagaatgcc gacgcccatc
    46201 atcatatact gacgagaagc gggtttactg gctgcttttt tgcgcttacg cggacgacgc
    46261 tctacgcgct cttcatccac ggtttcatct tcggattcgt caatttcttc ttcgatttcc
    46321 ggttcctcat tgcgctcttt ttgcgcacga gtcggacggc gatcgtcagt gtcatcaagt
    46381 tcaatatcat caaaattgat ctgcggttcg ccacgttcag tacgctcaga acgttcagaa
    46441 gattgacgag aacgaccagt acgacgatcg ctgggatcgg gtttcagctc gtcttctggt
    46501 ttgaattcat ccatttaaca ccccactaaa aggttaatgc ttaccacgtt gcaattaacc
    46561 tgaagctaat agaccgcttg ataagcggcc tgacctttct tgttgttacg ctgattgaca
    46621 atcggcaatg gcgttaagaa caagctcgtg cgaaacgccg ctgcgaactt cactcttacc
    46681 aattgccaac ggaagaatta agcgcatctc tcccgcaagg actttcttgt cacgcagcat
    46741 atgcggtaaa tacgcctgcg cggacatttc gcgcggccca ttgaccggta acccagcccg
    46801 cgtgagcagg gttataatac gctgcgtttc ggcagaacta aactgcccga gacgttccga
    46861 cgtccgcgcc gccatcacca tacccgcagc gaccgcttca ccatgtaacc aattgccata
    46921 ccccatttca gcttcaatgg catgaccaaa ggtgtgtccc agattcagta aagcacgtaa
    46981 cccggtttcg cgctcgtcgg cggcgacaac ttctgccttc agttcacaac aacggcgaat
    47041 acagtacgcc attgccggac cgtccagacg caacaacgca tccagattct cttccagcca
    47101 gttaaaaaac gcaccgtcaa gaataatgcc gtatttgatg acttctgcca gccccgacgc
    47161 taactcacgc gggggaagcg ttttcagaca gtcgagatcc accaccactg aagcaggctg
    47221 gtagaacgcg ccaatcatgt ttttaccgag gggatggttg accgcagttt tgccgccaac
    47281 ggaggaatcg acctgcgaca gtaacgtcgt cgggacttga atgaaacgaa caccgcgctg
    47341 ataactcgcc gccgcgaagc cggtcagatc gcccactacg ccgccgccaa gcgccaccag
    47401 cgtagtatcg cgaccatgcg gcttttgtaa caacgccgta aagacggtat cgagtacagc
    47461 taggctttta tactgctcgc cgtcagggag gataacgcta tcgacgttaa cacccgcctg
    47521 ttcaagtacg ccgcggacct tatcgagata cagaggagcc agggtttcgc tggtgaccaa
    47581 catgacctgc tcgcccgatt tcagcggtaa gaatgaagct ggttcattaa acaaaccaga
    47641 tgcgatggta attgggtaac tacgttcccc gagagtaacg acaatcctct ccataacgcg
    47701 acatccacct taattactgt acccgcagac gagtgtatat aaagccagaa ttagttgctt
    47761 tccagcatgt gaataatctg gtttgcaacc actttagcgc tttgatcatc agtacgaatg
    47821 gtcacgtcgg caatctcttc atacagcgga ttgcgttcat tggccaacgc ttccagaact
    47881 tcacgcggcg gtgtttcaac gtgcagcaac gggcgttttt tatcacgctg cgtgcgtgca
    47941 agttgctttt cgatggtcgt ttcaagataa acgacaacgc cacgagcgga aagacggtta
    48001 cgcgtttcac gggatttcac agagccgccg ccagtagcca gcacaatacc ctgtttctcg
    48061 gtcaactcat tgatgacctt ttcttcgcga tcgcggaagc cttcttcgcc ttctaaatcg
    48121 aaaacccagc ccacatcagc tccggttcgt ttctcaatct cttgatcgga atcgtaaaat
    48181 tccatattga gttgttgagc taactggcgc ccaatagtgc tttttccggc acccataggc
    48241 ccaaccagaa agatattgcg tttctctgcc attttttcgg tactactaag actattcgtt
    48301 aatgataaac ccgcttcgct cagagagcgc cgcaggacat gaactgaaac ctcataagat
    48361 attgcgagag tcagactgaa aattatctca atactccagc gggtttggca actgaataaa
    48421 tcacccatcc ctctgcatat ctggtcgctg caagcgcgct gccttgctac caccgctctg
    48481 gcgataaatc accgggtaag attagcgtaa aaaagacagc aaaatgccgc ctgaatgata
    48541 aatcatcatc atggaacgca ggcagcgaag agcaccaact ctcaaatcgg tactccttgt
    48601 atgctaaata cctgcgcgcg tcaaatagat gaaacacgtt cagcataaaa acattaccgc
    48661 ttttacggct gtttactcac tggaaaccag tcgtggcgtg ataaacacca ctaactcgcg
    48721 tcgttcatct tcttttccgt catgacgaaa taattgcccg aaccagggaa tgtcgccaag
    48781 caacggtacg ctatcctgac ccgatttatt tttacgggta aaaatgccgc ccagcgccaa
    48841 cgtttctccg ctttttacct cgacctgcgt ttcgatctcc tgcttatcaa tcgccagcac
    48901 ttcgccatcg gcctgctgta gcacctgccc cggaacgttc tggctgatgt gtaatttcag
    48961 ccggatgcga cctttttgta acaccgtggg cgtgacctcc atccccagga cggcctcttt
    49021 aaattccacc gacgtcgcgc cactttcccc gctggaaacc tgatatggaa tttcgctctc
    49081 ctgtttaatg ctggcaggct gaagatgtga ggccagcaga cgcggactgg cgataatatc
    49141 cagctgctgt ttttgttcga gcgcggaaag ctcaagatcc agcaagcgtc cgttgatgcg
    49201 cccaatgtta aaaccgacat gcgttgtcgc cgtcgctacg gagaggtcgc taccaagcgt
    49261 ggtgacttgc ccaacgccac cagcgtgttg cgcatcggcc agcgtccatt tcacgcctaa
    49321 ctcacgcaaa cttttttcat taatggtgac aatatgcgcc gacagctcaa cctgcccgac
    49381 cggcagatcc atttgcgcta cccactgttc aagcgcgctt aacgccgttt tgttatctcg
    49441 tagcaaaagg cgattggtgc gtttatcgac ggtcatactc cctttggcac tcagtagctt
    49501 ctcccccgct ttcgccagtt ctcccgcgtc ggcgtattgc agggttatac tgcgattttc
    49561 cagcggcaga tttgcctgcg cccgcgcctg ctccgcctcc tggcgggcga tattgtcatt
    49621 ctgccaggca atggaatgca ctgagagaat gttgccttcc tgccgcgtta tcagtccggc
    49681 gcttttcact acagtttgta gtgcctgctt ccagggaaca tctgttagat gtaacgacac
    49741 cgtaccgctg acgtctggcg acacgaccag gttcaacttc tcctgttcag ccagcgcctg
    49801 caacacctga gctaccggaa cgtcatccac catcagcgtc actttttgcg gctttgccgc
    49861 ctgtacgccg ggtatcagca tcaacagtag tgcggctatc cattgcttca tttgtatctc
    49921 cttgccgttg ccacaaccat tgtggcggtt cgcagtttgt cccggtaccc agcgttagta
    49981 cgtctggcgt cagctgtaaa attgtccagc cgttttccag cacatcgttt tgctgcaccc
    50041 gtcgccattt cttttgcccg tcttttatta caccgatgat gcgctcgcct cgccctacca
    50101 tcccctgata gcgccactgg ctaagttcgc taatccggca tatagatctt ccggcgattt
    50161 aaaagggtca cgcataccgg ttaaaaggca caatgcaata cctgccaaca accagcgttt
    50221 aaccctcatg cggcatctcc agttgtagcg tgaacaaaag atcatcacct tccacgctta
    50281 acgaaaaacg gctcacgctg acgttgcgct ctgccagccg tgtaaatgcc gacggcactg
    50341 cttcccacag cgttttcaac gccaactcac ctccctgcgc ggatggatgc caggaaacca
    50401 gttgcgcgcc ggataactga aaatccagtg gcgaaaaggg cagcgttttt tcctcgctga
    50461 agggagtggt gtctaccagg cgatacagtg cggcccactg ctggtgatga cttgcccgta
    50521 gtcgaattaa tgcgtcgcgc tcttcatggt gtgtcgatga tagaaaaatg agcgtaacta
    50581 acatcagcaa ccagaatgcc cagcaaaact ggcggaggcg gggtgatgtg gcgaaccacc
    50641 agtcaaagaa catgttcatc gctaaccttc cttgttaact gatactcaaa ttgccagcgt
    50701 ccctgcgcat cctgctgcgt ggctccccgc tgattgagat gaaaagacgc atcctggcgg
    50761 agtgacgttt ctagtgcgtt taacgcggta atgcttgttg tcagcccctt gatctccagc
    50821 gttccctgct gccagcttat cgttgtcagc caggcgtgct cgggtaaaag cgccgccagt
    50881 gcttccagcg cagattgcca gtcgcgggta aattgtcgct ggcgctgccg ttgcgaacgc
    50941 tgttcgcgta actgctgttg ctccagcaaa cgtggcttcg ttatctgtaa gctgcgggcg
    51001 agttgttgtt ccgcctgaag caaaactgcg tctatgcgcg cttcggcgct gcctgtcaga
    51061 cgcagtatta gcgttatccc gacggccagc agcagaggcg caacgaacat cagcaaccag
    51121 aaacgcagaa aagcggtccg gcgttgctgt cgccagggca aaaaattaat tggcggattc
    51181 atcagtacgc cttcccaagc gccagcccca gcgcgatggc aaagtctcca ccaggcggcg
    51241 gtagcggcgg ctgacgaaca gaaacggcct cccaggggtc aaatccgcct tcgccacata
    51301 tcgcgacgct gtcgggatca acggataacg ctgccgccag ctccttcgcg ctagtcatcc
    51361 ccaccgccag tttgcgcccc cagctatagc gcgtcgccca cagccactgt tcgttatcac
    51421 gccaggccag acattgctga tgagaaggta aaaaaggcag gaatcgctgt aatgcactgg
    51481 catccggggt gatcgcacta acatgaacac gcaacctttc tgccagcgta agcagcgttg
    51541 ccagctcttt gctttgcgcg gcagtcacgt tataagcggg gctgagtgag tcttcgctgt
    51601 aatcgaagcg cagggagtcc ggatccatat ccagctcgcg ggccatcgtc cctgacagcc
    51661 aggccgtttg ctcccgctca ccaagggaca tcgacgggcg cggaaatgac cgctgtaatg
    51721 tgcgactggc gggaaacgcc aacataatgt gatgacgctg cggcagttcg cgactccacg
    51781 gtaacaacgt tttagccagc tgctgcgcat caacaatccg cccatctttg ataatgtcgt
    51841 gcgccagcgg caaccgccac cagcgttgca aaaagcattc ttttgcgccc cgcacgatcg
    51901 caaccgctgc cgcttcttgc tgttgtaaat gcaaaccaat ttgccagatc ttaaatgtca
    51961 ttgtgatgat ctccttatca cccgtcactc tgacgggtat atcaatgcgt ctggcttgcc
    52021 tttatactac cgcgcgtttg tttataaact gcccaaatga aactaaatgg gaaatttcca
    52081 gtgaagttcg taaagtattt tttgatcctt gcagtctgtt gcattctgct gggagcaggc
    52141 tcgatttatg gcctataccg ctacatcgag ccacaactgc cggatgtggt gacattaaaa
    52201 gatgttcgcc tgcaaattcc gatgcagatt tacagcgccg atggcgagct gattgctcaa
    52261 tacggtgaga aacgtcgtat tccggttacg ttggatcaaa tcccaccgga gatggtgaaa
    52321 gcctttatcg cgacggaaga cagccgcttc tacgagcatc acggcgttga cccggtgggg
    52381 atcttccgtg cggcaagtgt ggcgctgttc tccggccacg cgtcacaagg ggcgagtacc
    52441 attacccagc agctggcgag aaatttcttc ctcagtccag aacgcacgat gatgcgtaag
    52501 attaaggaag tcttcctcgc gattcgcatt gaacagttgc tgacgaaaga cgaaatcctc
    52561 gagctttatc tgaacaagat ttaccttggt taccgcgcct atggtgtcgg tgctgcggca
    52621 caagtctatt tcggtaaaac ggtcgaccaa ctgacgctga acgaaatggc ggtgatagcc
    52681 gggttgccga aagcgccttc cacctttaat ccgctctact caatggatcg tgccgtcgcg
    52741 cggcgtaacg tcgtactgtc gcggatgctg gatgaagggt atatcaccca gcaacagttc
    52801 gatcagacac gcactgaggc gattaacgct aattatcacg cgccggagat tgctttctct
    52861 gcgccgtacc tgagcgaaat ggtgcgccag gagatgtata accgttatgg cgaaagtgcc
    52921 tatgaagacg gttatcgcat ttacaccacc atcacccgca aagtgcagca ggccgcgcag
    52981 caggcggtac gtaataacgt gctggactac gacatgcgcc acggctatcg cggcccggca
    53041 aatgtgctgt ggaaagtggg cgagtcggcg tgggataaca acaagattac cgatacgctg
    53101 aaggcgctgc cgacctatgg tccgctgctg cctgccgcag tcaccagcgc caatcctcag
    53161 ggagcgacgg cgatgctggc ggacgggtcg accgtcgcat tgagtatgga aggcgttcgc
    53221 tgggcgcgtc cttaccgttc ggatactcag cagggaccga cgccgcgtaa agtgaccgat
    53281 gttctgcaaa cgggtcagca aatctgggtt cgtcaggttg gcgatgcatg gtggctggca
    53341 caagtgccgg aagtgaactc ggcgctggtg tcgatcaatc cgcaaaatgg tgccgttatg
    53401 gcgctggtcg gtggctttga tttcaatcag agcaagttta accgcgccac ccaggcactg
    53461 cgtcaggtgg gttccaacat caaaccgttc ctctacaccg cggcgatgga taaaggtctg
    53521 acgctggcaa gtatgttgaa cgatgtgcca atttctcgct gggatgcagg tgccggttct
    53581 gactggcagc cgaagaactc accaccgcag tatgctggtc caattcgctt acgtcagggg
    53641 ctgggtcagt cgaaaaacgt ggtgatggta cgtgcaatgc gggcgatggg cgtcgactac
    53701 gctgcagaat atctgcaacg cttcggcttc ccggcgcaaa acattgtcca caccgaatcg
    53761 ctggcgctgg gttcagcgtc cttcacccca atgcaggtgg cgcgcggcta cgcggtcatg
    53821 gcgaacggcg gcttcctagt ggacccgtgg tttatcagca aaattgaaaa cgatcagggc
    53881 ggcgtgattt tcgaagcgaa accgaaagta gcctgcccgg aatgcgatat tccagtgatt
    53941 tacggtgata cgcagaaatc gaacgtgctg gaaaataacg atgttgaaga tgtcgctatc
    54001 tcccgcgagc agcagaatgt ttctgtacca atgccgcagc tggagcaggc aaatcaggcg
    54061 ttagtggcga agactggcgc gcaggagtac gcaccgcacg tcatcaacac tccgctggca
    54121 ttcctgatta agagtgcttt gaacaccaat atctttggtg aaccaggctg gcagggtacc
    54181 ggctggcgtg caggtcgtga tttgcagcgt cgcgatatcg gcgggaaaac cggtaccact
    54241 aacagttcga aagatgcgtg gttctcgggt tacggtccgg gcgttgtgac ctcggtctgg
    54301 attggctttg atgatcaccg tcgtaatctc ggtcatacaa cggcttccgg agcgattaaa
    54361 gatcagatct caggttacga aggcggtgcc aagagtgccc agcctgcatg ggacgcttat
    54421 atgaaagccg ttcttgaagg tgtgccggag cagccgctga cgccgcctcc gggtattgtg
    54481 acggtgaata tcgatcgcag caccgggcag ttagctaatg gtggcaacag ccgcgaagag
    54541 tatttcatcg aaggtacgca gccgacacaa caggcggtgc acgaggtggg aacgaccatt
    54601 atcgataatg gtgaggcaca ggaattgttc tgattaaaaa ggcgcttcgg cgccttttca
    54661 gttggctgac aaagtgcatt tgtttatgcc ggatgcgggt gaacgcgtta tccggctaac
    54721 aaaatcgtga aaaatcaata tattgcagga accccataag cctgataagc gttgtgcatc
    54781 aggcaaactt cacgcattta cactcgcccc tgccctttca accattcgcg cacgaggaac
    54841 agcgcgctga cattacgcgc ttcgttgaag tcagggtctt ccagcaaatc catcatatgc
    54901 gccagcggcc agcgcacctg tggtagcggc tctggctcat cgccttccag cgattccggg
    54961 tagagatctt gcgcgaccac gatattcatt ttgctggaaa agtaagacgg tgccatgctg
    55021 agcttcttca aaaaagtcag atcgttcgca ccaaatccaa cctcttcttt tagctcgcgg
    55081 ttagcggctt cgtagacgct ttcacccgga tcaattaatc ctttcgaaaa acctaattcg
    55141 taggattcag ttcccactgc gtattcgcgg atcaggatca ggtgatcgtc cacaatcggc
    55201 acaatcatca ctgcttcccg gttggttgga cgcattcgtt cataaacacg ccgcacgcca
    55261 ttgctgaact ccagatccac gctctcgacg gtaaacagtc gggaacgggc tacagtttca
    55321 acattcagaa tggtgggttt ttgtaatgat ttgttcatcg tgggatctat gctgtgaaat
    55381 cagcggttat tgtgcgatat cggacacgct ttcggcaatg tgaattgcat gttatttaca
    55441 tttatgtaac ttaataaata atcgtcctca aatcaaatta aaagtcaata ggttgaaata
    55501 actccaggaa tttgctgata ttccgccttc agagggtttg ctatgatcag cggttactgt
    55561 gatgtgctta atgatgctca agttaagctc cacgcttgcc gatagccaac cgcagaatca
    55621 tgtattgtgt ccggtgcgac tgaccacgcc tgacagacta agtaagatgg ggaaagcatg
    55681 agcaccattg tgattttttt agctgctttg ctggcctgct cactacttgc gggatggctg
    55741 ataaaagtgc gatccagacg gcgtcagctg ccctggacca acgccttcgc ggatgcgcaa
    55801 acgcgtaaac tcacacctga agaacgtagc gccgttgaaa attatcttga gagcctgacg
    55861 caggtattac aggtgcctgg cccaacggaa gctagcgcgg caccgatctc tctggcgctg
    55921 aatgccgaaa gcaacaacgt catgatgctg acacacgcta tcacacgtta cggcatctct
    55981 accgacgatc cgaataaatg gcgttactac ctcgattcgg tagaagtcca cctgcccccc
    56041 ttctgggaac agtacatcaa cgatgagaat accgttgaac tgattcatac cgattcgctg
    56101 ccgttggtta tttcactcaa cggtcatacg ctgcaggagt acatgcagga aacgcgcggc
    56161 tatgccttgc aacctgttcc gtccacgcag gcgtcgattc gcggggaaga gagtgagcaa
    56221 atcgagctac tcaatattcg caaagaaacg catgaagaat atgcgctgag tcgtccgcgc
    56281 gggctgcgtg aagcgttgct gatcgtcgcc tccttcctga tgttcttttt ctgcctgatt
    56341 accccggatg tatttgttcc gtggctggca ggcggcgcat tactgctgct gggcgcaggt
    56401 ctgtgggggc tattcgcgcc cccggcaaaa tcctccctgc gagaaattca ttgtctgcgc
    56461 ggtacacccc gtcgttgggg attgtttggc gaaaacgatc aggaacagat caacaatatt
    56521 tcactcggta ttatcgacct ggtctatccc gcacactggc agccatacat tgctcaggat
    56581 ctcggtcaac aaaccgatat tgatatctat ctcgaccgcc acgtagtgcg tcaggggcga
    56641 tatctttcgc tgcatgatga agtaaaaaac tttccgttac agcactggct gcgcagtacg
    56701 attatcgctg cgggctcgct gctagtgctg tttatgctgt tattctggat cccgctggat
    56761 atgccgctga aattcactct ctcatggatg aaaggcgcgc agaccattga agccaccagc
    56821 gtaaaacaac tggctgacgc tggcgtgcgg gtaggcgata cattgcgtat tagcggtacg
    56881 ggaatgtgta atattcgcac ttccggaacc tggagcgcga aaaccaattc acctttttta
    56941 ccgtttgact gctcgcagat catctggaat gacgcccgct cattaccatt accagaatct
    57001 gaactggtca acaaagcgac ggcattgact gaagcagtta atcgccagct gcaccctaaa
    57061 ccggaagatg aatctcgcgt cagtgcctca ttacgttcag cgattcaaaa atccggcatg
    57121 gtattgcttg atgattttgg cgacattgta ctgaagacag cggatttatg ttctgccaaa
    57181 gatgactgtg tgcgactgaa aaatgcgctg gtcaatctcg gcaacagtaa agactgggac
    57241 gcgctggtaa aacgcgccaa cgccgggaag ctcgatggcg tgaatgtgtt attacgcccg
    57301 gtgagtgctg aatcgctgga taacctggtg gcaacctcca ccgcgccgtt tatcacgcat
    57361 gaaacggcgc gagcggcaca atcactaaac agtccggccc ccggcggatt cctgattgtc
    57421 agcgacgaag gcagcgattt tgttgatcag ccctggcctt cggcatcact ttacgactac
    57481 ccgccgcaag aacagtggaa cgctttccag aaactggcac aaatgctgat gcatacgccg
    57541 tttaacgccg aaggtatcgt cacaaaaatc ttcactgacg ccaatggtac gcagcatatt
    57601 ggccttcatc cgatcccgga tcgttccggc ctgtggcgct atctcagcac cacattgctg
    57661 ctactgacga tgctgggtag cgccatttac aatggcgtac aggcctggcg tcgttaccag
    57721 cgtcatcgca ctcgcatgat ggagattcag gcctattatg aaagctgcct gaacccgcaa
    57781 ctgatcaccc cttcagaaag ccttatcgaa taacacgttt gcgcggcagg ttatgctacc
    57841 ctgtcgcgca aattgcttca ctctggagat ttccctcatg catatcaaca ttgcctggca
    57901 ggacgtagat accgttctgc tggatatgga cggcacgttg ctcgacctcg ccttcgataa
    57961 ctatttctgg caaaagctgg tgcctgaaac atggggcgcg aaaaacgggg ttacgccaca
    58021 ggaagcgatg gaatatatgc gccagcaata tcacgacgta cagcatacgc taaactggta
    58081 ctgtcttgat tactggagtg agcaactggg tctggatatc tgtgcgatga ccaccgagat
    58141 gggaccgcgc gccgtactgc gtgaagatac cattccgttt cttgaggcac tgaaagccag
    58201 cggtaagcag cgaattttgc tcaccaatgc tcatccgcac aacctggcgg taaaacttga
    58261 gcatacgggt ctggacgcac accttgattt attactttcc acccacacat ttggttatcc
    58321 gaaagaggat cagcggttat ggcatgcggt ggccgaagct acgggtctga aagctgaaag
    58381 aacgctgttt attgatgaca gcgaagcgat tctcgatgct gccgcgcaat ttggtattcg
    58441 ttactgcctc ggcgtgacta atcctgattc cgggattgcc gagaaacagt atcaacgcca
    58501 tccgtcactg aatgactacc gccgcctgat cccctcgcta atgtgaagga gacgccatga
    58561 aagagaaacc tgctgttgag gttcgactgg ataaatggct atgggctgcc cgtttttata
    58621 aaacccgcgc gctggcccgt gaaatgattg aaggcggtaa ggtgcattac aacgggcagc
    58681 gcagcaagcc gagcaaaatc gtcgagctga atgccacgct cactctgcgc cagggaaatg
    58741 acgaacgcac ggtgattgta aaggcgatta ctgaacagcg tcgccccgcc agcgaggcag
    58801 ccttgctgta tgaagagact gcggaaagtg tagagaaacg cgaaaaaatg gcgatggcac
    58861 gtaaacttaa tgccttaacc atgccgcacc cggaccgacg cccggacaaa aaagagcgcc
    58921 gcgacctgtt acgatttaaa cacggcgaca gtgaataact gtcacctgca agagagatga
    58981 ttatgccgca acatgaccaa ttacatcgct atctgtttga aaattttgcc gttcgcggcg
    59041 aactggtaac cgtttcggaa accctgcaac agatccttga gaaccacgat tatccgcagc
    59101 ccgttaaaaa cgtgctggca gaactgctgg ttgcgaccag cctgttaacc gctacgctga
    59161 agtttgatgg tgatatcacc gtacagctgc agggcgacgg tccgatgaat ctggcggtta
    59221 ttaacggtaa caataaccag caaatgcgcg gtgtggcgcg cgtgcagggc gaaattccag
    59281 aaaatgccga cctgaaaacg ctggtcggca atggttacgt ggtgatcacc attaccccga
    59341 gcgaaggcga acgctatcag ggcgtagttg gtctggaagg tgataccctg gcggcctgcc
    59401 tagaagatta ctttatgcgt tctgaacagc tgccgacgcg cctgtttatt cgcaccggcg
    59461 acgtagacgg caaaccggct gcaggcggta tgttgttgca ggtaatgcct gcgcaaaata
    59521 cccaacagga cgactttgac cacctggcga cgctaaccga aaccatcaaa accgaagaac
    59581 tgctgacctt accggcaaac gaagtgttgt ggcgtttgta tcacgaagaa gaggtgacgg
    59641 tttacgatcc gcaggatgtg gagttcaaat gcacctgctc gcgtgaacgt tgcgccgatg
    59701 cgctgaaaac gctgcctgat gaagaagttg atagcatcct ggcggaagat ggcgaaattg
    59761 acatgcattg tgattactgc ggtaaccact atctgttcaa tacgatggat attgctgaaa
    59821 tccgcaacaa cgcgtctccg gcagatccgt aagttcatta atgagtatgt ccggcagaga
    59881 gggctctgcc ggattttacg acttaatggc ctgatgtgct gcgcttacca gcgcctatga
    59941 aataaatcgc aaattatttt ggtcagatac agcgtcgtat ccgataaaag cgttgtcact
    60001 tacttaagtt tatttccagc gtttcaccat ctaccgtcag atcgcgccac gctttcggcc
    60061 tcgtccccag ttttccatcc ttcgcagctg tgagtaattt ttctttcgtt atttcgttcg
    60121 gaagtaaact catatcaagc gcgtcccatt cttttctgtc tgagtcaaag ccatagacaa
    60181 tgactctgtc atcattaaaa gcaaacagga tccgctccgc ctgaccatca ctattcagat
    60241 cttgctcgac cagtacgcag gcgtcttttt caatacaggt catcacgtta tagcgatcct
    60301 ggattaaggc cgaccagaat gtcgcatccg gtttaacaga accaggggca attaacacat
    60361 tttcggcgaa tactttttcc gatacctgtt cctgaagatg ttgctctcca tctaacgcca
    60421 tcagcagatc ccgcgcgcgt ttcgggtctt tcataaaccc ggcatcgctt ttcagcgact
    60481 caagcgccgc acgtccatag cgaccgctct gttcgagcat gtagatagtt acctggtctg
    60541 gcgtgttttt gccgctctga taacgcgcca tatgactgtt cacgctaata cgcatactgt
    60601 ccagcaccgg cgaattaaga agcaccagta tcaccaacac caataacgaa accgcaaggt
    60661 tcactttgcc ctgaagaaca aggggatttt gcccattacg ccagacgata ctgacaaaat
    60721 accccagcga ccacaccagt aacaccagct ccgccagcgc gccctgcaag cggtcgacag
    60781 tccagccgta ctgagcgacc cgcagccata acccccaggt ggcgacgaac acatacagcg
    60841 gagcaaccag caaagcggtt ttaatcaggc aacgcaacgg ccccgtccag ggaagtgacg
    60901 ctttttgcgg atcgcggaca atagccatta agatcaattg caaaaaggcc agcgttaaca
    60961 gcaacccggc ggcggagatg tggcgagaaa tcgcgctcag gcccgtaaac ggcagggtga
    61021 tgataaacat cagggttagc aatgatacta acggcagcaa ccccgtggcg attagcgtga
    61081 acaacttttg aatagagtcg attaaacgtg actgtgtacg cgcgaggatc accgccagtg
    61141 cggtaaccag accaaatgta agatacataa accagtcggt tgcaaaaaag agcgtattga
    61201 aaaacgtgat gccaacaagt ttaaacaact caccccacag taaaagcacc agccacgtca
    61261 ggccattgga gagaaaaatc accagtaata taagtacgtt atgccatact gactggtaaa
    61321 agtagctata gcggctactg ccattgcgga tacgaaggct ttgttgtatc cacggtagca
    61381 acaacattgc catcaacagc agatagcagc caaaatccca aatcgccttt tcagctctcc
    61441 aggggttcat gccatcagtt tgccacttca gccaaccgct catccctgtc gtggcaataa
    61501 acaccagcgc cagccatccc caaaggcgct tctgtttaaa agagatcacg gagaagagta
    61561 aaacggatga aaacgcgacg gtcgcaggca cgccataaac aatccaactg tgattttttc
    61621 cggcaagcca ggcgataagc aggtagcaaa ccagcccctg aagtaatccg gtagcaatca
    61681 tcccctgaag taatccggta gcaatcatcc cccagcgtgt tgccggtgaa agttcaacgt
    61741 tatccatgtt ggttatccag aatcaaaagg tgggttaatt atcgcatccg ggcagtagta
    61801 ttttgctttt ttcagaaaat aatcaaaaaa agttagcgtg gtgaatcgat actttaccgg
    61861 ttgaatttgc atcaatttca ttcaggaatg cgattccact cacaatattc ccgccatata
    61921 aaccaagatt taaccttttg agaacatttt ccacacctaa aatgctattt ctgcgataat
    61981 agcaaccgtt tcgtgacagg aatcacggag ttttttgtca aatatgaatt tctccagata
    62041 cgtaaatcta tgagccttgt cgcggttaac acccccaaaa agactttact attcaggcaa
    62101 tacatattgg ctaaggagca gtgaaatgcg cgttaacaat ggtttgcccc cgcaagaact
    62161 cgaggcttat ggtatcagtg acgtacatga tatcgtttac aacccaagct acgacctgct
    62221 gtatcaggaa gagctcgatc cgagcctgac aggttatgag cgcggggtgt taactaatct
    62281 gggtgccgtt gccgtcgata ccgggatctt caccggtcgt tcaccaaaag ataagtatat
    62341 cgtccgtgac gataccactc gcgatacttt ctggtgggca gacaaaggca aaggtaagaa
    62401 cgacaacaaa cctctctctc cggaaacctg gcagcatctg aaaggcctgg tgaccaggca
    62461 gctttccggc aaacgtctgt tcgttgtcga cgctttctgt ggtgcgaacc cggatactcg
    62521 tctttccgtc cgtttcatca ccgaagtggc ctggcaggcg cattttgtca aaaacatgtt
    62581 tattcgcccg agcgatgaag agctggcagg tttcaaacca gactttatcg ttatgaacgg
    62641 cgcgaagtgc actaacccgc agtggaaaga acagggtctg aactccgaaa acttcgtggc
    62701 gtttaacctg accgagcgca tgcagctgat tggcggcacc tggtacggcg gcgaaatgaa
    62761 gaaagggatg ttctcgatga tgaactacct gctgccgctg aaaggtatcg cttctatgca
    62821 ctgctccgcc aacgttggcg agaaaggcga tgttgcggtg ttcttcggcc tttccggcac
    62881 cggtaaaacc accctttcca ccgacccgaa acgccgcctg attggcgatg acgaacacgg
    62941 ctgggacgat gacggcgtgt ttaatttcga aggcggctgc tacgcaaaaa ctatcaagct
    63001 gtcgaaagaa gcggaacctg aaatctacaa cgctatccgt cgtgatgcgc tgctggaaaa
    63061 cgtcaccgtg cgtgaagatg gcactatcga ctttgatgat ggttcaaaaa ccgagaacac
    63121 ccgcgtttct tatccaatct atcacatcga taacatcgtt aagccggttt ccaaagcggg
    63181 ccacgcgact aaggttatct tcctgactgc tgatgctttc ggcgtgttgc cgccggtttc
    63241 tcgcctgact gccgatcaaa cccagtatca cttcctctct ggcttcaccg ccaaactggc
    63301 cggtactgag cgtggcatca ccgaaccgac gccaaccttc tccgcttgct tcggcgcggc
    63361 gttcctgtcg ctgcacccga ctcagtacgc agaagtgctg gtgaaacgta tgcaggcggc
    63421 gggcgcgcag gcttatctgg ttaacactgg ctggaacggc actggcaaac gtatctcgat
    63481 taaagatacc cgcgccatta tcgacgccat cctcaacggt tcgctggata atgcagaaac
    63541 cttcactctg ccgatgttta acctggcgat cccaaccgaa ctgccgggcg tagacacgaa
    63601 gattctcgat ccgcgtaaca cctacgcttc tccggaacag tggcaggaaa aagccgaaac
    63661 cctggcgaaa ctgtttatcg acaacttcga taaatacacc gacacccctg cgggtgccgc
    63721 gctggtagcg gctggtccga aactgtaatg atttgaagct ggagaatatc tatccagtat
    63781 cttatagaaa gcaaaacggg aggcaccttc gcctcccgtt tatttaccct tcttttgtcg
    63841 tgccctgcgc ccgcgttacc ggcactggca gccaggcgcg aatggaaagc ccgccccgct
    63901 cgctggtgcc aagctccagc atcccgttat ggttatccac gatacgctgc acaattgcca
    63961 gccctaatcc cgtgccgcta atggtgcgcg cactgtcgcc gcggacaaac ggctggaaca
    64021 ggtgcttacg ttgttccggc gcaattcccg gaccgtcatc ttccacctgg aaccaggcgc
    64081 gattcggctc cgttccgctg ctgactttga tccagccatt gccataacgg gcggcgttga
    64141 ccaccatatt cgccaccgcg cgtttgatcg acagcgggtg cattttcact tcaatgctgc
    64201 cggggtaaag cgcggtttca atttcccgct catagccact ttcggcagca atcacctcac
    64261 cgagcactgc attaagatcc gccatttcca tcggcatctc ctgcccggtg cgcaggtagt
    64321 cgataaactg ctcaatgatg gcgttgcact cttcgatatc tttattgatc gattctgcca
    64381 gatagccatc ctgctcgctc atcatctcag tcgccaggcg aatacgcgtc agcggcgtgc
    64441 gcaagtcgtg acttaccccc gccatcagca gcgtgcggtc atccgccagt tgcttaacac
    64501 cagccgccat atggttaaag gcacgggtaa cggaacgcac ctccgaagcg ccatattcac
    64561 gcagcggcgg cggaataatc cctttaccaa cctgcaaggc tgcgtgttcg agatcgacca
    64621 acggtcggtt ctggatacga ataaacagcc acgccccgcc tatcgccaat agcataatcg
    64681 ccagcgtata gcggaacagc ggagagaaat cgccctgatg aatttcggtc agcggcacgc
    64741 gtacccagat attgggcgac agccaggttt tcagccagac gacaggcgaa cttttgttga
    64801 cctcaacgcg cacttccgtc gggccgccca gttgctgtgc catctgatgg cttaagaatt
    64861 catagtgttg cgcccaacgc agacctgcct cttcggcagc ctcgttggag tagagagaga
    64921 tccccagctc acggtagatc tcccgacgga aagcgggagg cacaaccaac tgcgtgccgt
    64981 cctccagttg cagtttgtcg gtcatcaaca tacgcacttc gtacgcgagg actttattaa
    65041 actgctggag gctcggcaaa atcgcgaagt tcagcaccac cagataagtc gtcaccaggc
    65101 tggcgaacag caaggtgacg atgagcaata acgtacgggc aaatgaactt cgtggcgaga
    65161 agcgcaatcg cctcatgctt tagagccgtc cggtacaaag acgtagccca ggccccaaac
    65221 ggtctgaatg taacgcggat gcgctggatc ttcttctacc atgcggcgca gacgcgaaat
    65281 ctgcacgtcg atggagcgtt ccattgcgga atattcacga ccacgggcaa ggttcatcag
    65341 cttatcgcgg gagagcggct cacgcggatg gctgaccagt gccttcagta ccgcaaactc
    65401 accgctggtg agcggcatcg gctcatcttc gcggaacatt tcgcgcgtac cgaggttaag
    65461 tttgaactta ccgaaagcaa ttaccgcctc ttcctgtgac ggtgcgcctg gcagttcgtt
    65521 cgcctgacga cgcagcaccg cacgaatacg ggccagcagt tcacgcgggt taaacggttt
    65581 tggaatgtag tcgtcagcgc caatctccag gcctacgata cggtccactt cttccccttt
    65641 cgccgtcacc ataatgatcg gcatcgggtt gctctgacta cgaagacgtc ggcaaatcga
    65701 caagccatct tcaccaggta acattaaatc cagtaccata agatggaaag attcacgagt
    65761 cagcaggcga tccatctgtt ctgcattagc gacgcttcga acctggaagc cttgttcggt
    65821 gagataacgt tccagcagcg cacgcaggcg catgtcgtca tcgaccacca gaatcttgta
    65881 gttctcttgc attgtttgta ctcccaaagg ttcgcaacaa tttgtaagcg tgtattctta
    65941 aaaaagctca cgttcgtcac cagctaaatc tggtatgaat ttcagcctaa attgttacaa
    66001 agcatattaa acagcagctt aagtatacaa tttattcggc gaaacattat tgattctgtt
    66061 gatatgatca cgttataccc aatgtgcgca ttatcaaaca gacaaaggga atcaacgaga
    66121 tgaaaacgcc cctggttacc cgggaagggt atgaaaaact caaacaagag cttaattatc
    66181 tctggcgtga agaacgcccg gaggtcacaa aaaaggtgac ctgggccgca agtctgggcg
    66241 accgcagcga aaatgctgac tatcagtata ataaaaagcg tctgcgtgaa atcgaccgtc
    66301 gcgtgcgcta tctcactaaa tgcctggaaa atctcaaaat cgtcgattac tcccctcagc
    66361 aggaaggcaa agtctttttt ggcgcgtggg tggagattga aaacgacgat ggcgtgactc
    66421 accgtttccg tattgtcggc tacgatgaaa tttttggccg taaagattac atctctatcg
    66481 attccccgat ggcccgcgca ttgctgaaaa aagaagtcgg cgatctggcg gtggtgaata
    66541 cccctgccgg ggaagcgaac tggtatgtta atgctatcga gtacgtgaaa ccgtaaggaa
    66601 gagtcttaac ctcctgccga tggctggcat ttttgccagc cagtccgtat aactatcccc
    66661 tgatttttga tccgaaaaga tgaactcaaa ccatgatgaa tgattcgttc tgccgcatta
    66721 ttgcgggtga aattcaggcg cgcccggaac aggttgacgc tgccgttcgc ctgcttgacg
    66781 aagggaatac cgtgccgttt atcgcacgtt atcgtaagga aatcaccggc ggtctggatg
    66841 acacgcagct gcgtaatctg gaaacgcgtc tgagctatct gcgcgagctg gaagagagac
    66901 gtcaggcgat cctcaaatcc atttccgagc aaggcaaact caccgatgat ctggcgaagg
    66961 ccatcaacgc caccctaagc aaaaccgaac tcgaagacct ctacctgccc tacaaaccta
    67021 aacgccgcac ccgcgggcaa atcgccattg aagcagggct tgagccgttg gctgacctgc
    67081 tgtggagcga tccgtcacac acgccagaag tcgccgctgc acaatatgtt gatgccgata
    67141 aaggcgtggc agataccaaa gccgcgctgg atggcgcgcg ctatatcctg atggaacggt
    67201 ttgccgaaga tgccgcgctg ctggcgaaag tgcgtgatta tctgtggaag aacgcgcatt
    67261 tggtttctac ggtggtgagc ggtaaagaag aggaaggggc gaaattccgc gactatttcg
    67321 atcatcacga accgttgtcc acggtgcctt ctcaccgcgc gctggcgatg ttccgtgggc
    67381 gtaacgaagg cgtactccag ctttcgctga atgccgatcc gcagttcgat gagccgccca
    67441 aagagagcta ttgcgagcaa atcatcatgg atcaccttgg cctgcgcctg aacaatgccc
    67501 cggcggatag ctggcgcaaa agcgtagtga gctggacctg gcgcatcaag gtgctgatgc
    67561 atctggaaac cgaactgatg ggtaccgtgc gcgaacgcgc ggaagatgaa gcaatcaacg
    67621 tctttgcccg taacctgcac gatctgctga tggcggcccc tgccggactg cgtgcaacga
    67681 tgggcctcga tccgggtctg cgtaccgggg taaaagtggc ggtggtcgat gccactggca
    67741 aactggtggc gaccgatacc atttacccgc acaccggaca agccgcaaaa gcagcgatga
    67801 ccgttgctgc cttgtgtgaa aaacataacg ttgaactggt ggcgatcggc aacggtacag
    67861 cttcccgcga aactgaacgt ttctatctcg acgtgcagaa gcagttcccg aaagtgaccg
    67921 cacagaaagt gatcgtcagc gaagctggcg cgtcggttta ctcagcttcc gagctggcag
    67981 cgcaggagtt cccggatctc gacgtttcgc tgcgtggtgc ggtatctatc gcccgccgtt
    68041 tgcaggatcc gctggcggag ctggtgaaaa tcgatccgaa atctatcggc gtaggtcagt
    68101 atcagcatga cgtcagccag acgcaactgg cccgcaaact ggatgcagta gtagaagact
    68161 gcgtaaacgc cgttggcgtc gatctcaaca ccgcttctgt tccgctatta acccgcgtgg
    68221 cgggcctgac gcgcatgatg gcgcaaaaca tcgtggcctg gcgcgatgag aacggccagt
    68281 tccagaaccg tcagcaactg ctgaaagtca gccgtctggg gccgaaagcc ttcgagcagt
    68341 gcgcgggctt cctgcgcatt aaccacggtg ataacccgct ggacgcctct accgttcatc
    68401 cggaagccta tccggtggtg gaacgcattc tggcagcaac acagcaggca ctgaaagatc
    68461 tgatgggtaa cagcagcgaa ctgcgtaacc tgaaagcgtc tgactttact gatgaaaaat
    68521 tcggtgtgcc gacagtaact gacatcatca aagagctgga aaaaccgggt cgcgatccgc
    68581 gtccggaatt taaaaccgct cagtttgccg atggcgtcga gacaatgaac gacctgcaac
    68641 cgggtatgat cctcgaaggc gcagtgacca acgtcaccaa ctttggcgcg tttgtcgata
    68701 ttggcgtgca tcaggacggc ctggttcaca tctcttcatt gtcgaacaag tttgtggaag
    68761 atccgcatac cgtggtgaaa gcgggcgaca ttgtgaaggt gaaagtgctg gaagtggatc
    68821 ttcagcgtaa acgtatcgcc ctgactatgc gtctggacga gcagcctggc gaaaccaact
    68881 ctcgtcgcgg cggcggtaat gaacgcccgc aaaacaaccg cccggcagcc aaaccacgcg
    68941 gtcgtgaagc gcagcctgcc ggtaatagcg cgatgatgga tgcgctggcg gcagcgatgg
    69001 gtaaaaaacg ttaaacgccc ggactggccc ctaaacctgc gcgataaggc gttcacgccg
    69061 catctggcaa cgaacgcttt gcctgacata aaagtgccgg agaatatctc cggcactttt
    69121 atgtcaggca aactcacaat atactccgtt aatatttatc acttcattaa caactgaaac
    69181 cttaattaaa cattagccag tccgggtaac tcactattcg aattatattt tcgctgcgat
    69241 ataaccttga gccacatcaa cattgagtca gattattatt caaaccaaca ttcgcacaca
    69301 ttttaagtat tgctgataga aaccattctc attatcattg tgttgttgat tatttaatct
    69361 ctcctttgtt ggcaaatcat ctggtctcat gtcgcggtca aacgccccat gaggtagtta
    69421 tccagttaat gagaaacaag taggcaccta tgcaatacac tccagatact gcgtggaaaa
    69481 tcactggctt ttcccgtgaa atcagcccgg catatcgcca aaaactgctt tctctaggca
    69541 tgttacctgg ctcctctttt gatgtggtgc gcgtcgctcc actcggcgac cccattcata
    69601 tcgaaacccg tcgtgtgagc ctggtattac gcaaaaaaga tctggcctta ttagaagtgg
    69661 aagcggtttc ctgttaatac ggtgataaca acaatgaaaa aattaaccat tggcttaatt
    69721 ggtaatccaa attctggcaa gacaacctta tttaaccagc tcactggcgc acgtcagcgt
    69781 gtaggtaact gggctggcgt taccgtcgaa cgtaaagaag ggcaattctc caccaccgat
    69841 catcaggtca cgctggtgga cctgcccggc acctattctc tgaccaccat ctcatcgcag
    69901 acctcgctcg atgagcaaat cgcctgtcac tacattttga gtggcgacgc cgacctgctg
    69961 attaacgtgg tggatgcgtc taaccttgag cgtaacctgt acctgacgct gcaactgctg
    70021 gaactcggca ttccctgcat cgtggcgctg aacatgctcg acattgccga gaagcaaaat
    70081 attcgtattg aaattgatgc tctatcggca cgtcttggct gcccggtgat cccgctggtt
    70141 tcaacccgtg gtcgcggtat tgaagcgctc aagctggcga ttgatcgcta taaagctaac
    70201 gagaatgtgg aactggtgca ttacgcacag ccgctactca acgaagcaga ttcactggca
    70261 aaagtgatgc cttccgacat cccgctgaaa caacgtcgct ggctgggcct gcaaatgctg
    70321 gaaggcgata tctacagccg cgcctacgcc ggtgaagcgt cgcagcatct ggatgccacc
    70381 ctcgcccgtc tgcgtaatga aatggatgat ccggcgctgc acattgccga tgcgcgttac
    70441 cagtgcattg ctgccatctg tgatgtggta agcaacaccc tgacggcaga acccagccgt
    70501 ttcaccactg cggtagataa aatcgtgctc aaccgtttcc tcggtctgcc gattttcctc
    70561 tttgtgatgt acctgatgtt cctgctggct atcaacatcg gcggggcgtt acagccgctg
    70621 tttaacgtcg gctccgtggc gctatttgtg catggtattc aatggattgg ctacacgctc
    70681 cacttcccgg actggctgac tatcttcctc gcccagggcc tgggtggcgg tattaacacc
    70741 gtgctgccac tggtgccgca gatcggcatg atgtacctgt tcctctcctt ccttgaggac
    70801 tccggctata tggcgcgtgc agcgtttgtg atggaccgcc tgatgcaggc gctgggcttg
    70861 ccggggaaat cctttgtgcc gctgatcgtc ggtttcggtt gtaacgtacc gtcggtaatg
    70921 ggcgcacgta cgcttgatgc accgcgtgaa cgtctgatga ccatcatgat ggcaccgttt
    70981 atgtcctgcg gcgcgcgtct ggctatcttc gcagtattcg cggctgcctt cttcgggcag
    71041 aacggtgccc tggcggtttt ctcgctgtat atgctcggta tcgtgatggc ggtgctgact
    71101 ggcctgatgc tcaagtacac catcatgcgc ggtgaagcga cgccgtttgt catggagctg
    71161 ccggtctacc atgtaccaca cgttaaaagc ctgattatcc agacctggca gcgtctgaag
    71221 ggcttcgttc tgcgtgctgg taaagtgatt atcatcgtca gcattttcct gagcgctttc
    71281 aacagcttct cgttgagcgg gaaaatcgtc gataacatca acgactcggc gctggcttcc
    71341 gtcagccggg tgataacccc tgtcttcaag ccgattggcg tacatgaaga taactggcag
    71401 gcaacggttg gcctgtttac gggcgcaatg gcaaaagaag tggtggtggg tacgctcaac
    71461 accctctaca ccgcagaaaa tattcaggac gaagagttca atccggcgga attcaacctc
    71521 ggtgaagagc tgttcagtgc ggtagatgaa acctggcaga gcctgaaaga caccttcagc
    71581 cttagcgtac tgatgaaccc cattgaagct agcaaaggca acggtgaaat gggtaccggg
    71641 gcgatgggcg tgatggatca gaaattcggt agcgcagctg ccgcatacag ctacctgatt
    71701 ttcgtcctgc tgtatgtacc atgtatctcg gtgatgggag ccatcgcccg tgaatcaagc
    71761 cgtggctgga tgggcttctc catcctgtgg gggctgaata tcgcttactc actggcaaca
    71821 ttgttctatc aggtcgccag ctacagtcag cacccaactt acagcctggt gtgcattctg
    71881 gcggtaatcc tgtttaacat cgtagctatc ggtctgctgc gccgcgcgcg tagccgggtg
    71941 gatatcgaac tgctggcaac ccgcaagtcg gtaagcagtt gctgcgcagc cagcaccacc
    72001 ggtgattgcc attaatggct tcacttattc aggtacgtga tttgctggcg ttacggggcc
    72061 gtatggaagc gacccagata agccagacat tgaacactcc gcagccaatg attaacgcca
    72121 tgctgcaaca gctggaaagc atgggcaaag ccgtgcggat tcaggaagaa cctgacggct
    72181 gcctctctgg cagttgtaaa agctgcccgg aaggcaaagc ctgtctgcgc gagtggtggg
    72241 cgctgcgtta accatactcc atcgcctgtt ttgaaaagcc cggtatgcgt ttgcatccgg
    72301 gcttttttgc gtgcggcttt ccataaaaat gcaattcttg cagcgcaacg taagttcctc
    72361 tgaaagcgtc tcgcagggat gaaaactcgc taatgcacag atgtggagtg gcgcgtagag
    72421 tcgcggcatt caaacaacag gtgaaggaac gccatgagta aaaagcagag ttccacccca
    72481 catgatgcgc tgttcaaact ctttttacgc caaccggaga cggcacgcga ttttcttgcg
    72541 tttcatttac cggcacccat tcacgcgctc tgtgatatga aaaccctcaa gctggagtcg
    72601 aggagcttta ttgatgacga tctgcgtgaa agctattccg atgtgctgtg gtcggtgaaa
    72661 acggaacaag gaccaggata catctattgt ctgattgaac atcaaagcac ctcaaacaaa
    72721 ctgatcgcat ttcgcatgat gcgttacgct attgccgcaa tgcaaaatca ccttgatgct
    72781 ggatacaaaa cgttgccgat ggtggtgcca ttgttgtttt accacggtat tgaaagcccc
    72841 tatccctatt cgctgtgttg gctggattgt ttcgccgatc ccaaactggc aaggcagctt
    72901 tatgcctccg catttccgct gattgatgtc accgtcatgc ctgatgatga aatcatgcag
    72961 caccgacgta tggcgctgct ggagttaatt caaaaacata ttcgtcaacg cgatctgatg
    73021 gggctggtag agcaaatggc ctgcttatta agtagtggat acgctaatga cagacaaatc
    73081 aaagggctgt ttaattacat actgcaaact ggcgacgctg tacgttttaa cgattttatc
    73141 gacggcgttg ccgaacgttc accgaaacac aaggagagtt taatgactat tgcggaaaga
    73201 ttgcggcagg agggggaaca atccaaagcc ctgcatatag ccaaaataat gcttgaatcc
    73261 ggagtccctc ttgcagacat catgcgattt accgggctgt cagaagaaga gttggctgcg
    73321 g
//
LOCUS       DAJTSA010000005        71478 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000005 DAJTSA010000000
VERSION     DAJTSA010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 71478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.005"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(753..2339)
                     /locus_tag="OO369_000355"
     CDS             complement(753..2339)
                     /locus_tag="OO369_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HCR5980753.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
     gene            complement(2694..4865)
                     /gene="glcB"
                     /locus_tag="OO369_000356"
     CDS             complement(2694..4865)
                     /gene="glcB"
                     /locus_tag="OO369_000356"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCR5980754.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(4887..5291)
                     /locus_tag="OO369_000357"
     CDS             complement(4887..5291)
                     /locus_tag="OO369_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCR5980755.1"
                     /translation="MKTKVILSQQMSSAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5296..6515)
                     /gene="glcF"
                     /locus_tag="OO369_000358"
                     /pseudo
     CDS             complement(5296..6515)
                     /gene="glcF"
                     /locus_tag="OO369_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(6526..7578)
                     /gene="glcE"
                     /locus_tag="OO369_000359"
     CDS             complement(6526..7578)
                     /gene="glcE"
                     /locus_tag="OO369_000359"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCR5980756.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(7578..9077)
                     /gene="glcD"
                     /locus_tag="OO369_000360"
                     /pseudo
     CDS             complement(7578..9077)
                     /gene="glcD"
                     /locus_tag="OO369_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9328..10098
                     /gene="glcC"
                     /locus_tag="OO369_000361"
     CDS             9328..10098
                     /gene="glcC"
                     /locus_tag="OO369_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCR5980757.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10222..11363)
                     /locus_tag="OO369_000362"
                     /pseudo
     CDS             complement(10222..11363)
                     /locus_tag="OO369_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            11725..13455
                     /locus_tag="OO369_000363"
     CDS             11725..13455
                     /locus_tag="OO369_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCR5980758.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            13452..14366
                     /locus_tag="OO369_000364"
     CDS             13452..14366
                     /locus_tag="OO369_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR5980759.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            14399..14647
                     /locus_tag="OO369_000365"
     CDS             14399..14647
                     /locus_tag="OO369_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5980760.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            14647..15818
                     /locus_tag="OO369_000366"
                     /pseudo
     CDS             14647..15818
                     /locus_tag="OO369_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(15854..16933)
                     /gene="lptG"
                     /locus_tag="OO369_000367"
     CDS             complement(15854..16933)
                     /gene="lptG"
                     /locus_tag="OO369_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5980761.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(16930..18000)
                     /gene="lptF"
                     /locus_tag="OO369_000368"
     CDS             complement(16930..18000)
                     /gene="lptF"
                     /locus_tag="OO369_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5980762.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18031..18591)
                     /locus_tag="OO369_000369"
     CDS             complement(18031..18591)
                     /locus_tag="OO369_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5980763.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(18603..19427)
                     /locus_tag="OO369_000370"
     CDS             complement(18603..19427)
                     /locus_tag="OO369_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5980764.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(19427..20776)
                     /locus_tag="OO369_000371"
     CDS             complement(19427..20776)
                     /locus_tag="OO369_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR5980765.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(20822..21580)
                     /locus_tag="OO369_000372"
     CDS             complement(20822..21580)
                     /locus_tag="OO369_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5980766.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(21612..22325)
                     /locus_tag="OO369_000373"
     CDS             complement(21612..22325)
                     /locus_tag="OO369_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5980767.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            22499..23191
                     /locus_tag="OO369_000374"
     CDS             22499..23191
                     /locus_tag="OO369_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5980768.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23240..24739)
                     /gene="pitB"
                     /locus_tag="OO369_000375"
     CDS             complement(23240..24739)
                     /gene="pitB"
                     /locus_tag="OO369_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR5980769.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25031..26890)
                     /gene="gss"
                     /locus_tag="OO369_000376"
     CDS             complement(25031..26890)
                     /gene="gss"
                     /locus_tag="OO369_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR5980770.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27095..27961
                     /gene="yghU"
                     /locus_tag="OO369_000377"
     CDS             27095..27961
                     /gene="yghU"
                     /locus_tag="OO369_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR5980771.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28084..28332)
                     /gene="hybG"
                     /locus_tag="OO369_000378"
     CDS             complement(28084..28332)
                     /gene="hybG"
                     /locus_tag="OO369_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR5980772.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(28466..28807)
                     /gene="hypA"
                     /locus_tag="OO369_000379"
     CDS             complement(28466..28807)
                     /gene="hypA"
                     /locus_tag="OO369_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5980773.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(28800..29288)
                     /gene="hybE"
                     /locus_tag="OO369_000380"
     CDS             complement(28800..29288)
                     /gene="hybE"
                     /locus_tag="OO369_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR5980774.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29281..29775)
                     /locus_tag="OO369_000381"
     CDS             complement(29281..29775)
                     /locus_tag="OO369_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR5980775.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(29775..31478)
                     /gene="hybC"
                     /locus_tag="OO369_000382"
     CDS             complement(29775..31478)
                     /gene="hybC"
                     /locus_tag="OO369_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR5980776.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(31475..32653)
                     /gene="hybB"
                     /locus_tag="OO369_000383"
     CDS             complement(31475..32653)
                     /gene="hybB"
                     /locus_tag="OO369_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR5980777.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(32643..33629)
                     /gene="hybA"
                     /locus_tag="OO369_000384"
     CDS             complement(32643..33629)
                     /gene="hybA"
                     /locus_tag="OO369_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR5980778.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(33632..34750)
                     /gene="hybO"
                     /locus_tag="OO369_000385"
     CDS             complement(33632..34750)
                     /gene="hybO"
                     /locus_tag="OO369_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR5980779.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(34939..35226)
                     /locus_tag="OO369_000386"
     CDS             complement(34939..35226)
                     /locus_tag="OO369_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR5980780.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35345..36232)
                     /locus_tag="OO369_000387"
     CDS             complement(35345..36232)
                     /locus_tag="OO369_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5980781.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            36389..37429
                     /gene="gpr"
                     /locus_tag="OO369_000388"
     CDS             36389..37429
                     /gene="gpr"
                     /locus_tag="OO369_000388"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR5980782.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(37469..37963)
                     /locus_tag="OO369_000389"
     CDS             complement(37469..37963)
                     /locus_tag="OO369_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR5980783.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38154..39038
                     /gene="yghA"
                     /locus_tag="OO369_000390"
     CDS             38154..39038
                     /gene="yghA"
                     /locus_tag="OO369_000390"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5980784.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39310..39735)
                     /gene="exbD"
                     /locus_tag="OO369_000391"
     CDS             complement(39310..39735)
                     /gene="exbD"
                     /locus_tag="OO369_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR5980785.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(39742..40476)
                     /gene="exbB"
                     /locus_tag="OO369_000392"
     CDS             complement(39742..40476)
                     /gene="exbB"
                     /locus_tag="OO369_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5980786.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            40728..41915
                     /gene="metC"
                     /locus_tag="OO369_000393"
     CDS             40728..41915
                     /gene="metC"
                     /locus_tag="OO369_000393"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR5980787.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVIKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42055..42714
                     /locus_tag="OO369_000394"
     CDS             42055..42714
                     /locus_tag="OO369_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5980788.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(42754..43653)
                     /gene="yqhC"
                     /locus_tag="OO369_000395"
     CDS             complement(42754..43653)
                     /gene="yqhC"
                     /locus_tag="OO369_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR5980789.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            43847..45010
                     /gene="yqhD"
                     /locus_tag="OO369_000396"
     CDS             43847..45010
                     /gene="yqhD"
                     /locus_tag="OO369_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR5980790.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45115..45942
                     /gene="dkgA"
                     /locus_tag="OO369_000397"
     CDS             45115..45942
                     /gene="dkgA"
                     /locus_tag="OO369_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR5980791.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46142..47068
                     /locus_tag="OO369_000398"
     CDS             46142..47068
                     /locus_tag="OO369_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR5980792.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47119..47376
                     /gene="yqhH"
                     /locus_tag="OO369_000399"
     CDS             47119..47376
                     /gene="yqhH"
                     /locus_tag="OO369_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR5980793.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(47419..49638)
                     /locus_tag="OO369_000400"
     CDS             complement(47419..49638)
                     /locus_tag="OO369_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR5980794.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(49749..51161)
                     /gene="ftsP"
                     /locus_tag="OO369_000401"
     CDS             complement(49749..51161)
                     /gene="ftsP"
                     /locus_tag="OO369_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR5980795.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51236..51973)
                     /gene="plsC"
                     /locus_tag="OO369_000402"
                     /gene_synonym="parF"
     CDS             complement(51236..51973)
                     /gene="plsC"
                     /locus_tag="OO369_000402"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR5980796.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52207..54465)
                     /gene="parC"
                     /locus_tag="OO369_000403"
     CDS             complement(52207..54465)
                     /gene="parC"
                     /locus_tag="OO369_000403"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR5980797.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(55011..55493)
                     /locus_tag="OO369_000404"
     CDS             complement(55011..55493)
                     /locus_tag="OO369_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR5980798.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(55546..55938)
                     /gene="ygiW"
                     /locus_tag="OO369_000405"
     CDS             complement(55546..55938)
                     /gene="ygiW"
                     /locus_tag="OO369_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR5980799.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56090..56749
                     /gene="qseB"
                     /locus_tag="OO369_000406"
     CDS             56090..56749
                     /gene="qseB"
                     /locus_tag="OO369_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR5980800.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            56746..58020
                     /gene="qseC"
                     /locus_tag="OO369_000407"
     CDS             56746..58020
                     /gene="qseC"
                     /locus_tag="OO369_000407"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR5980801.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(58066..58398)
                     /locus_tag="OO369_000408"
     CDS             complement(58066..58398)
                     /locus_tag="OO369_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCR5980802.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            58717..59298
                     /gene="mdaB"
                     /locus_tag="OO369_000409"
     CDS             58717..59298
                     /gene="mdaB"
                     /locus_tag="OO369_000409"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR5980803.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59329..59643
                     /locus_tag="OO369_000410"
     CDS             59329..59643
                     /locus_tag="OO369_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5980804.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(59691..61583)
                     /gene="parE"
                     /locus_tag="OO369_000411"
     CDS             complement(59691..61583)
                     /gene="parE"
                     /locus_tag="OO369_000411"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR5980805.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(61612..62193)
                     /gene="yqiA"
                     /locus_tag="OO369_000412"
     CDS             complement(61612..62193)
                     /gene="yqiA"
                     /locus_tag="OO369_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR5980806.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62193..63020)
                     /gene="cpdA"
                     /locus_tag="OO369_000413"
     CDS             complement(62193..63020)
                     /gene="cpdA"
                     /locus_tag="OO369_000413"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR5980807.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(63045..63467)
                     /locus_tag="OO369_000414"
     CDS             complement(63045..63467)
                     /locus_tag="OO369_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR5980808.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(63468..64097)
                     /gene="nudF"
                     /locus_tag="OO369_000415"
     CDS             complement(63468..64097)
                     /gene="nudF"
                     /locus_tag="OO369_000415"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR5980809.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64302..65783
                     /gene="tolC"
                     /locus_tag="OO369_000416"
     CDS             64302..65783
                     /gene="tolC"
                     /locus_tag="OO369_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR5980810.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            65931..66602
                     /locus_tag="OO369_000417"
     CDS             65931..66602
                     /locus_tag="OO369_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR5980811.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            66608..67768
                     /locus_tag="OO369_000418"
     CDS             66608..67768
                     /locus_tag="OO369_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5980812.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPCGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(67806..68594)
                     /gene="ygiD"
                     /locus_tag="OO369_000419"
     CDS             complement(67806..68594)
                     /gene="ygiD"
                     /locus_tag="OO369_000419"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR5980813.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            68737..69510
                     /gene="zupT"
                     /locus_tag="OO369_000420"
     CDS             68737..69510
                     /gene="zupT"
                     /locus_tag="OO369_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR5980814.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(69568..69738)
                     /locus_tag="OO369_000421"
     CDS             complement(69568..69738)
                     /locus_tag="OO369_000421"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5980815.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(69999..70652)
                     /gene="ribB"
                     /locus_tag="OO369_000422"
     CDS             complement(69999..70652)
                     /gene="ribB"
                     /locus_tag="OO369_000422"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR5980816.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(70756..70904)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            71026..71316
                     /gene="ubiK"
                     /locus_tag="OO369_000423"
     CDS             71026..71316
                     /gene="ubiK"
                     /locus_tag="OO369_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR5980817.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        17835 a        19053 c        17654 g        16936 t
ORIGIN      
        1 ttttcattta ttcacaccta ataaaagtat tcaaattcag atgatttttc tgctcaacag
       61 acacaaacag gcaataatac tctccaaagc gaacaatatt tggcattacg atttaatgct
      121 tcataatcac caaattattt aaccataaat tttcttattt ctaacaaaat gattggtgtg
      181 ttgttggagt taaaatacat acataatatt aacatctgcc aacatttcac tttccgcaag
      241 acggatgatt tcgaatcctc atccacgtta ccctatccca aatatcttta ttaataggca
      301 gtaacgaaca ctgttatagc aagattaact tattaaccta acggcatctt ttatgtaaca
      361 agtggagata aattgcttaa aattgatcct cgacaaatct taaaagttct cattattctg
      421 caaaatcagc tttgcgaata atcttatgtt tttgggagtt ttgggaattt tgatctactc
      481 attgttctag tgaaaaatat aaataagaga aaaaacgcca ttcgtcatga aagcaacaga
      541 tgaagcaatg taataacata ctaaaattga tctcatgaaa gtgggtactt tataaccagc
      601 acagaagcca agttgtataa atattaaaca attcgcatcg taaaacggag actacgtata
      661 aagaagccaa tcgtaaaaac ggttccctgg cgagataaac gacagacaaa tggaaagccg
      721 ggtgatattt accttcatcc ggctttattg atttacgaga ctaacatccc ggtaaacaca
      781 tacgcctgca gcagggtgat aacgctggca aaaatcagac tgtgcttcac ggtgtagcgg
      841 aacagttcag attctcggcc caccatgccc gtcgcggcgc aggccacggc gatagattgc
      901 ggggagatca tcttgccagt tacgccgccg ctggtatttg ctgccaccag cagagtgtca
      961 gagacgttga tttgctgcgc cgtggtcgat tgcagtgaac caaacagggc gtttgaagaa
     1021 gtgtccgacc cggtaaggaa tacgcccagc cagccgagaa acggtgagaa aaacgggaac
     1081 atcacgcctg tacctgccag taccagcgcc agcgtggtgg acatgccgga atagttggtg
     1141 acgaaggcga atgccagcac catgccaatc gacaatatcg gccacttcag actaattagc
     1201 atttcggcaa agacgccaat ccctttcttg atgcccacgc cgaggatgaa gatagagata
     1261 atcgccgcaa taaaaatagc ggtgccacca gccgagagag ggtcgaattt aaacaccgca
     1321 tccattggcg ttggttgggc gacaatgggt gccgctttca acacttgttg atgcaaatga
     1381 gggatctgga aattaatcac cagtgaataa aacgcgccgc ccggagcaaa taacgcttta
     1441 aacggcttca tggtccagat ggtgaccagc accgttaaga ttaaaaacgg tgaccacgca
     1501 cgaatgattt gccccagact atattctgaa ggcacgggac cgccagaaga tggcttattt
     1561 accaccatcg cacctgcgga ttgccccatg ctgattgccg tttcggtatt tttcggccgc
     1621 cagactttaa ggaataaagc gagtgagacg atactcacca gcgccgaagt aatatccggc
     1681 agttccggac caatatagtt agaggtaaag aactgagtga cagcgaagct tcccccagca
     1741 accagcgccg ctggccacgt ctctttcacc cctttccagc cgtccatcat tgctaccagc
     1801 cagaacggca caagaaccga caggaacggt aactgacgtc ccgccattgc gccaatgtgg
     1861 aacggatcga ttcccgttac ctgaccggcg accagaatcg gcacgcccaa cgcaccaaac
     1921 gccaccgtcg caccaaagcc agcagctcct tccagcaacg caccaaagga gaaaccaatc
     1981 agtaacacct gcaaacgctg atcgtcggtg atggagataa ccgagctgcg gataatatcg
     2041 aactgcccgc tggcaacggt taatttatac aggaacaccg ccgcgacaat aatccacgca
     2101 attggccata atccataaat aaagccatag cccgcagcag caaatgccat atcaatcggc
     2161 attttaaagg cgaatatcgc aatcaggata gataatataa gggttattgc tccagcaaca
     2221 tgtcctttca gacgtaatac cgcgagtgca acgaagaaga atattatcgg gatcagggcg
     2281 accagagcgg atagccccag tcctcccatc ggcatataca tttgggtcca ggtaaccata
     2341 ttgttcagtc tcttattatc tttatatgct tgatatactt aaggttgtaa taagcaaaag
     2401 aggactgaac tgtaaaatat aggcgttata ctttacagca acagtacgcc gctaacgcaa
     2461 ttgctacctc tggcataaca agtatatcgg gtaagggttt ctgttccgca cacgcaggcg
     2521 cagagtatcg ttaagatgtc catattgttg ttttaggccc gctagtaatg cgctacgggt
     2581 atttaatatt gttaaaccct gataatcgct ccggttattt ccgggataaa tgtactaccg
     2641 cagttactat catagccccg acaataaaac ttgccggggc ttttttgacg ctattaatga
     2701 ctttcttttt cgcgtaaacg ccaggcgtgt aataacggtt cggtataacc gtttggctgt
     2761 ttcacgccga ggaagattaa atcgctggca gctttaaaag cacacgagtt agcgaaattc
     2821 cccgccatcg gacgataagc cggatcgcca gcattttgct gatcaaccac tttcgccata
     2881 ttctccagcg acgcctgcac ctgttctttg gtcagaatac cgtgacgtaa ccagttggcg
     2941 atatgctggc tggagatacg cagcgttgcg cggtcttcca tcagcgccac gttgtgaata
     3001 tccggcactt ttgaacaacc aatcccctgc tccacccaac gcaccacgta ccccagaatc
     3061 ccctgcacgt tgttatccag ctcttgttgg atctcttgcg ccgaccagtt agcgttttca
     3121 gccaccggaa tagtcagcag atcgtccagc agcggttcaa attcagcact gaactcggtc
     3181 tgggcaatgt tggcttgcac gctctgtacg ttggtatggt ggtagtgcag cgcatggagc
     3241 gtagcagcgg ttggtgacgg aacccaggct gtgtttgccc cggcacgcag ttggtcgccc
     3301 ttctggctgt acatgtctgc catcaggtcc ggcattgccc acatgccttt accgatttgc
     3361 gctttaccgc gcagcccaca gaacagaccg gaaagcacgt tattacgttc gtaggctttg
     3421 atccacggcg tcgatttcat ctggttttta cgcagcatcg ggccagcttc catcaccgaa
     3481 tgcatttcat cgccggtacg gtcgaggaaa ccggtattga tgaacgccac gcggttgcgc
     3541 gcctgagcga tacagctacg caagttcagc gaggtccggc gttcttcatc cataatgccc
     3601 attttcaggg tattcggtgc cataccgagc attgtctcaa tgcgggtaaa cagtttgttg
     3661 gcgaacgcca cttcctgcgg accgtgcatt ttcggtttca caatatagac gctgccagtg
     3721 cgcgagtttt tctgcacttt taaatcatag agggcaatcg cgccagtcat gacgccatca
     3781 agaatacctt ccgggatttc attgccttcg ctgtcccaaa tcacaggaat ggtcatcaaa
     3841 tgacccacgt tgcggataaa cagcaacgag cgtccgtgca gagaaatttc agagccatcg
     3901 gcggcggtgt actgacgatc gtcattcagt ttacgtacga tttgccgacc gtttttctcc
     3961 attttctctt gcagagtccc ctgcatcagg cccagcagat tgcggtacag caggatttta
     4021 tcttccgcat caaccgccgc gaccgaatct tcgcagtcga gaatggtact gatagcagcc
     4081 tcgacgataa catcgttgat gtgcgccgga tcgtctttgc caatccgccc gttggcatca
     4141 atttgcagtt caatatgcag gccgttattt ttcagcaaaa tgcaggtcgg cgcagcggca
     4201 tcgccacggt aaccgacaaa ctgtgctgga gtacgtaacg tggtttcttt accatttttc
     4261 aactggatgc gtaattgttt atcgaccacc ttaaacgcca ccacatcctg atagctgccg
     4321 ttttccagcg gtagtgattc atcgaggaaa cgtcgaaccc aggcgataac ctgctcaccg
     4381 cgctgcggat cgtagccact gaccatcgcc ccttcctgcg ggatgatgtc gctgccgtat
     4441 aacgcatcgt acagtgagcc ccagcgagcg ttcgccgcgt tcagcgcgta gcgggcgttc
     4501 attgccggaa ccaccagctg tggccccgcc tgactggtga tttcgctgtc aatgcccgtg
     4561 gtttccaccg tcacgcgctc cggttgcggc accaggtagc ccagttcacg aaggaaagat
     4621 ttataggccg ctttatcttt taccggcccc ggattgctgc gatgccactc atcaagcgcc
     4681 gcctgaatgc gatcgcgttc tgccagcaac tgacgatttt ctggtgccag atcgtgaacg
     4741 atctcatcaa cattgtgcca gaatgcagca gcgtccagtt ccacgcccgg caaaacttct
     4801 ccatccacaa aacgtttaaa attggcgtca atgcgtaaac ggccctgggt tatggtttga
     4861 ctcattgttt ctctcctcgt tttcgcttat ttcgccaaca ccgctgctgc cactttcgcg
     4921 acctgcgcat cctgtgctcc ggttaaacca gaaacgccca cggcaccaat aatttgccca
     4981 tcaacaacaa ccggtacgcc gccttccagc gacgtcagta acggcgcagt cacgaacgcg
     5041 gtacgtccgt tgttcaccat ctcttcatag cccttagttt cacgacgccc cagcgcggcg
     5101 gtacgcgctt tctcctggga gatgtaagcc gcaatcggcg cgcaatcgtc catgcgactt
     5161 aacgccagca gatgaccgcc gtcatcggca acagcaatgg aaacagacca gttatttttc
     5221 tgcgcctctt cctgacctgc ggcaataatt gcactcgaca tttgctggct aagaatgact
     5281 ttagttttca tttttttatt ccttttcaag ggcttgttct acaatttcaa tccagtgacg
     5341 cacagaggta cgaccggcgc tcgccagatg cgtctggcaa ccaatgttgg cggtgacgat
     5401 catttccggt ttgccgcttt ccagcgcatt cattttgtta tcccgcagct ggcgtgccag
     5461 atcgggatgc gttaacgcat atgttcccgc tgaaccgcag cacagatggc tgtcgggaac
     5521 gtccgttaag gtaaatccaa gacgaagtaa cactttttcc acttcgccgt tcagcttttg
     5581 cgcatgttgt agggtacacg ggcagtggaa ggccagtttt ttatcgccgc gaattgccag
     5641 tttttccagc ggttcctcgc gcagaagttc gactaaatcg accgccagtt cactgacctg
     5701 acgcgcttta tcggcatata acgcatcgtt tttcagcatc tgcccatact ctttgacgaa
     5761 cgcgccgcaa ccgctggcgg tttgcaaaat tgcctcggca cctgcttcaa tcgcgggcca
     5821 ccaggcatca atattattgc gcgcccgtgc caaccctttc tcctgcgcat taagatgata
     5881 gtccaccgcg ccacaacagc ctgcttcgtt agctgacatg acgctgatcc ccagacgatc
     5941 cagcactcgc gcagttgccg cgttggtgtt gggcgaaagc gtaggctggg cgcagccttc
     6001 cagcattaaa acccgacgct tatggcgcag cggcggacgc ggtttagctt tcaccgtttc
     6061 agcaggcagt tttgctctga cctgttccgg taaaaacggt cgcagcacca gccctacctg
     6121 cgtcagcgca cggaagaccg ccggacgcgg cactacctgg cgcaatcctt cgcgcagcat
     6181 tcgctccggc agtgggcgtt tcactttctg ctcgacaata tcacgcccga tatccagcaa
     6241 attgtgatag cgcacaccag aaggacaggt ggtttcacaa ttgcggcaag tgaggcagcg
     6301 atcgagatgc tcctgtgttt taagcgtgac ttcgttgcct tccagcacct gtttaatcag
     6361 atagatgcgc ccgcgcggcc cgtccagttc atcgcccaga agctgatagg ttgggcaggt
     6421 tgcggtacaa aatccgcagt gaacacaggc gcacaggatg ctgtcggctt ccagcgcgcg
     6481 ttctgccgca tctcttcggt taatcgggtt tgcatagcct gctcctcaaa gttccgcgta
     6541 catgcgaccg gggttaaaca cgccgcaagg gtcgagctgc tgtttaagct gctggtgata
     6601 gcggaataaa ggagccgata gcggggcaaa gccaccatct ccggcactaa agcgggtcgc
     6661 atgaccgcca gcgttgcggg cgatgcgatg gatttgattg tcatcggctg tcgatttcag
     6721 ccagcgtaac gccccgcccc agtcgatcag ttgctcgccg ggtaaatcca tcatcggcgc
     6781 atcgctgggt aatgaaatgc gccataaggt acctggtaac gagaagaacg gcagttgttg
     6841 ttcacgcaat tgctgccaga actgacccac aacctcttcg ccacccagca gttcacgcgc
     6901 tgcttttacc gatccttcgc cgccctcaag gcggatccac aacgcattgt cgaagtaaca
     6961 taagccacta atgggtaatg gctggagttg ccactcggcg atttcactca tggcttcttg
     7021 caggctgatt tcccgacgca ggctcagaga ggcacgcggt cgcggtaaca ctttcattga
     7081 gatttcagtg agcacgccaa gacaaccgta gcttccggcc attaaccgtg agagatcgta
     7141 tccggcaacg tttttcatca cttcgccacc aaaacgcaga tgttttccag tgccggtaat
     7201 gatgcgcgtg ccgaggacaa aatcgcggac cgaaccgctc cacgggcgac gcggccccgc
     7261 cagcccgcag gcgaccatcc cgccccaggt ggcttcttca ccataatgcg gcggctcgca
     7321 gggaagcatt tgcccggcgc tctccagcgc cgcttcaatc gccaccagcg gcgttccggc
     7381 acgagcggtt atcaccagct cggtcgggtc gtaattaaca atgccgcgat gacaacgcac
     7441 atccagcgtt tgcccggtga ctgggcgacc taaaaaggct ttgctattgc tgccctgaat
     7501 caccagcggc gttttatcgc taattgcctg attcacctgc tccagcagcg cctgactgta
     7561 atcacactcg cgtagcatca aaagcgctcc agttcaggaa aaggtaaatg accgtgatgc
     7621 acatgcatgg caccaaattc agcacagcgg tgtagcgtgg gaatgttttt cccagggttc
     7681 agcaaaccat cggggtcaaa cgccgccttg accgcatgga aggtcgtgat ttcatcgctg
     7741 ttgaactggg cgcacatttg attgattttt tctcgcccga tgccatgttc cccactgatg
     7801 ctgccgccaa cttcaacgca gagttcgagg atcttcccgc ccagctcttc cgcgcgggca
     7861 aattcaccag gttcgttggc atcgaaaagg attaacgggt gcatgttgcc atctccggca
     7921 tgaaagacgt tggcaacacg taaatcatat tgctgcgata aacgggcaat gccttccagt
     7981 acgccaggca gggcgcgacg cgggatggtg ccatccatgc agtagtaatc cggggagata
     8041 cgtcctaccg ccgggaacgc atttttgcga ccggcccaga aacgtacgcg ctctgcttcg
     8101 tcctgtgcca aacggacgtc agtcgcgccc gctttcaaca agatgtagtt aacccgctcg
     8161 cagtcttcct gtacgtcaga ctccacgccg tccagttcgc ataacaaaat cgcttcggcg
     8221 tcgacgggat aaccggcatg aataaaatct tccgccgcgc ggatcgacag gttatccatc
     8281 atctccagcc cgccggggat aatgccattg gcgatgatgt caccaaccgc aagtccggct
     8341 ttttctaccg agtcaaagct ggctaacaga acccgcgcca cgggcggctt cggcagcagt
     8401 tttaccgtca cttcggtggt cacgccgagc ataccttccg atccggtgaa cagcgccagc
     8461 aggtcaaaac caggtgaatc cagcgcgtcc gatcccagcg tcagcgcctc gccgtccagc
     8521 gtttgcactt caattttcag caggttatgt acggtcagac catatttcag gcagtggacg
     8581 ccaccggcat tttccgccac attgccgcca attgaacagg cgatttgtga ggaaggatcc
     8641 ggcgcgtaat agagattatg cggcgcaacg gcctaggaga tcgccaggtt acatacgccc
     8701 ggctgcacgc gcgcgcggcg accaacgggg ttaatgtcga ggctctcttt aaagcgcgcc
     8761 atcaccaaca acacaccttt ttccagcggc agcgcgccac cagaaagccc ggtgcctgca
     8821 ccacgggtca ccaccggtac acgcaggcga tggcagacag ccagaatcgc tgtcacctgt
     8881 tccatttgct taggcagaac aaccagtaat ggacgcgtgc gatacgcgct caacccgtca
     8941 cactcgtaag gaatgatctc ctcatcggta tgcaggatct caagtccagg gacatgctca
     9001 cgcagtgcca tcagtaccga tgtgcggtcg acatcgggta aagcgccatc aagacgctct
     9061 tcgtacaaga tgctcatgag taggcttcgc tttgttgtgt tgtgtggcag ctgatttttg
     9121 cgcgctgctt ctgtgaacag ttattaagcg ggcttttcgt tttcgtctat ctctttagct
     9181 accggtcaga ccattttttt tccagctctg tgaccttgtc ttggttaact taatgttaaa
     9241 ttgatgtaac ataatcactt gcgtgatgtg cgtgttttgc gagttaagaa cagaaaaatt
     9301 ggtcctacct gtgcacgagg tccgggaatg aaagatgaac gtcgccctat ttgcgaagtg
     9361 gttgcagaga gtatcgaacg gttaattatc gacggcgtac tgaaggtcgg tcagccgctt
     9421 ccctcggaac gtcgactgtg tgaaaagctc ggcttctcac gctccgcact gcgtgaaggg
     9481 ctgaccgtgc tgcgcgggcg cgggattatt gaaacggcgc agggtcgcga ttctcatgtc
     9541 gcacggctta atcgggagca ggacaccagc ccgctgatcc atctgttcag tacgcagccg
     9601 cgaacgctgt acgatctgct cgacgttcgc gcattactgg agggcgaatc ggcaaggctg
     9661 gcggcaacgc tgggaacgca ggctgatttt gttgtgataa cccgctgtta tgaaaaaatg
     9721 ctcgccgcca gtgagaacca caaagagatt tcgctgatcg aacatgcgca gttggatcac
     9781 gctttccatc tcgccatttg tcaggcttct cacaatcagg tgctggtgtt tacgctgcaa
     9841 tcattgaccg atctgatgtt taattcagtg tttgccagcg taaataatct ctaccatcga
     9901 ccacagcaaa aaaagcagat cgatcgccag catgcgcgga tctacaacgc ggtgttgcag
     9961 cggctgccgc acgtcgctca gcgcgcagcg cgcgatcatg tacggaccgt gaaaaagaat
    10021 ctccacgata tcgagctgga aggccaccat ttgattcgct cggcggtgcc gctggagatg
    10081 aacaaggtgg gaatgtgagc agagttttac cctatcccca aggcgagggg accgcccctg
    10141 cacttaacag gatctggtgt taaatctgtt catctatacc ctctcccgaa tgaccgcccc
    10201 tgcgctaaac aggatctggt attaaatttg cttttcatac aatcggtaac gcttgtacgg
    10261 ctccgcccca atgcgttcca gcatgttatt catgcctgta ttggtttcga ggatccatga
    10321 catctccagc gcatcgatct tccggcgggc aaacggatcg cgtaaggctt caatcaataa
    10381 cagcgcaatc accgggccga tgcggctgaa ctgatactcg tcgcgcacgc ccatcagcgg
    10441 tactcgcgca gttcgcacac cgctgacttt caaacgccac agcaattttg cccagccgaa
    10501 gggaaagagc gatccgttca gatcggcaat cgcctcgttg atgttaggca agccacaata
    10561 aacgcgcagg gtgcagaatc aatctcagcg atgtagatca tatcgtccgg caccagatat
    10621 ttaagttggt cgcccatggt cgcgaattca tgttcggtaa acggcacaaa tccccagttg
    10681 tgctgccagc cagagttgaa aatctcacgc aggatctgca tctcttcggc aaaccgctga
    10741 cgattgatgc agcgaatggt cacctttttg cgcacctgat ccatcagttt ttttagcgcc
    10801 ggagagaaag tgagatcggt tcgctgcatc caccacgcca gtaaatcaat gcctttgtga
    10861 taacccagtt gttcaatatg cgcggcatac cacggtttgc cgtgtggcat catcgcacag
    10921 ggtggtgtgt caaaaccttc aatcagtaat ccgctttcct gattgatatt caggctgaaa
    10981 ggcccgctga tcttacttgc accttgtgac ttcaaccacg cttccgctgc gccaaacaac
    11041 gcggcaaaat cctgcggatc atcaatggcg tcaatcatgc cgaaatgacc ggtatcttca
    11101 ccgtaacgct cacggtgcaa ggtatctatt tgcgcggtaa tacgcccaac tatctgcccc
    11161 gctttttttg ccacccacgc ctgccagatg atatggtccg tccccggatt tttcgcagac
    11221 aaatgctcgt tgcgttcaat gaataaaggg ggtatccagt ttggatcgtc gggataaagt
    11281 gatgacggaa aagcgataaa tgccttaagg tcatttttat taattacttt ttcaatttta
    11341 atcatcagca gatcgcattc catatctctg tggataacaa ataattaatg gtgaaaaaca
    11401 ggttgcaaca gcaacgagcg gcatcttaat aatcaactca atgaatacaa acgacgaata
    11461 aatagtatta attaatgatt atgataaggt cagaggctac acagaaacgc ctcaatgcct
    11521 tttgtcaatt taaggcgacc ggacaaggct tcttcttaat atattcataa attaaatatc
    11581 attcataata aaaacaaaaa acttaaactc attatttaaa tcaatgttca ttttcacacc
    11641 gatgaaaata ctatctcatt tatcttttat gcctatataa tccaccgcat tattgtcatt
    11701 ttatttttga tatccaattt tttcgtggtg tatatgtcta ataaaatctt tacgcattcc
    11761 ctacctatgc gctatgccga ttttccaacg ctggttgatg ctttggacta cgccgctctg
    11821 agtagagccg gaatgaattt ttatgacaga cgttgccaac ttgaagatca actggaatat
    11881 cagacattaa aaacgcgtgc cgaagctggt gcgaagcggt tgttatcgct gaacctgaaa
    11941 aaaggcgaac gcgtggcgct gattgccgaa acgagtagcg ggtttgtaga ggcttttttt
    12001 gcctgtcagt atgctggctt agtcgccgtc ccgttggcga ttccaatggg cgtcggtcag
    12061 cgggattcct ggagcgccaa actccagggt ttactggcaa gttgccagcc cgcagccatt
    12121 atcactggtg atgagtggtt gccactggtc aatgccgcga cgcataacaa ccccgaatta
    12181 catgttttaa gccacgcttg gtttaaggca ttaccggaag ccgatgttgc gctccagcgt
    12241 ccagttccga acgatatcgc ctacctccag tacacctccg gcagcacccg ttttccccgt
    12301 ggcgtcatta tcacccatcg cgaagtgatg gctaatctac gtgctataag ccacgatggc
    12361 attaaattac gccctagcga ccgctgcgtc tcctggcagc ctttctacca tgatatggga
    12421 ctggtcggct ttctcctgcc ccccgtcgcc acgcaacttt cagtagatta tttgcgcact
    12481 caggattttg ccatgcgtcc tttgcaatgg cttaaattga tcagtaaaaa tcgcggcacc
    12541 gtttccgttg cgccgccgtt tggctatgaa ttgtgccaac gccgcgtgaa tgaaaaagat
    12601 ctcgctgaac tggatctttc ctgctggcgc gtcgctggta ttggtgctga gccgatctcc
    12661 gcagaacaac tccatcaatt cgctgaatgt ttccgtcagg ttaactttga caataaaact
    12721 ttcatgccgt gctacggact ggcagaaaat gcgctggctg tcagcttctc tgatgaagcc
    12781 tccggggttg tggttaacga agtggatcgc gacatcctcg aatatcaggg taaagccgtc
    12841 gcgccgggtg cagagacacg cgccgtatcg actttcgtca actgcggcaa agcgttgccg
    12901 gaacatggta ttgaaatccg caatgaagca ggtatgccgg tcgcggaacg tgtggtaggc
    12961 catatttgca tctccggtcc cagtctgatg agcggttact ttggcgacca ggtttcgcaa
    13021 gacgagattg ccgcgacggg ctggttagac accggcgacc tcggttatct gctggacggt
    13081 tatctgtatg tcaccggacg cattaaagat ctgattatta ttcgtggccg taatatctgg
    13141 ccgcaggata ttgaatatat agcggaacag gaaccggaaa ttcattctgg cgatgcgatt
    13201 gcttttgtta ccgcccagga aaaaatcatt ttgcagatcc agtgtcggat cagcgacgaa
    13261 gaacgtcgcg ggcagcttat ccacgcgctg gcggcacgga tccaaagcga atttggcgtg
    13321 accgcggcta tcgatctgtt gccgccccac agtattcccc gaacgtcctc cggcaagcct
    13381 gcccgtgcgg aagcgaaaaa acgttatcag aaggcttatg ctgccagtct taatgtgcag
    13441 gaatccctgg cataaatcaa actgtcgcgg tgacgggcgc taccgggttt atcggtaaat
    13501 atattatcga taacctgctc gcccgcggct ttcacgttcg cgcattgacg cgtactgccc
    13561 gcgctcacgt caatgataat ctcacctggg tgcgcggttc gctggaagat actcattcac
    13621 ttagcgagct ggttgccgga gccagcgtag tggtccattg cgccgggcaa gtgcgcgggc
    13681 acaaagaaga gattttcacc cgctgtaacg ttgacggcag cctgcgcctg atgcaagcag
    13741 caaaagagag cggcttttgc caacgttttc tgtttatctc ttcgctggcg gcgcgccatc
    13801 ccgagctctc ctggtatgca aaatccaaat acgtcgccga gcaacgactg gcagcaatgg
    13861 cggacgaaat tacgctgggc gtttttcgcc cgacagccgt gtatggtccc ggcgataaag
    13921 agttaaaacc gctgtttgac tggatgctac gcggcctgct gccacgactt ggtgcgccag
    13981 atacacaact ctcttttctt cacgtcaccg attttgcgca agcagtgggt cagtggttaa
    14041 gcgccgaaac tgtacagacg caaacctatg aattatgcga tggcgtcgcc ggtggctatg
    14101 actggcaacg cgtacgacag cttgccgcca aagcccgttg tggttccgtg cgaatggttg
    14161 acattcctct gccggtactc acaggccttg cggatatcag taccgcgttg agtcgcctgg
    14221 caggtaaaga acctatgctg acccgctcga aaattcgtga attaacccac gccgactggt
    14281 cggcaagtaa taaccgtatt tctgaagata tcaattggtt tcccgggatt agcctggaac
    14341 acgcattacg caacgggcta ttttgattgt cttgttttaa aggtgtgtta acgtttctat
    14401 ggtaaatcgt gaaatagtaa tggattacat cctttcctgt ttacaggatt tggtcgaaaa
    14461 cggtgtggaa attaagcccg acagcgatct cgtcaacgat cttggcctgg agtcgatcaa
    14521 agtgatggat ctcttaatga tgctggaaga cagattcgat atttccattc ctattaatat
    14581 tctgctggat gtcaaaaccc ctgcgcaatt aatggaaact ctacttccat ggctggagaa
    14641 taaataatgg ggctatacga taaatatgcg cgccttgctg gcgagcgtct gcaatttagt
    14701 gacaacgggt taacaccgtt tggcacctgt attgatgaag tgtattccgc caccgaaggc
    14761 cggatcggca ataaaaaagt gattctggcc gggaccaata attatctcgg tttaaccttt
    14821 aatcatgatg ccatcagcga aggtcaggcc gcgttagcgg cccaaggcac cggcaccacc
    14881 ggttctcgta tggcaaacgg cagttatgcg ccgcacctgg cgttagagaa agagattgcc
    14941 gagtttttca atcgccctac cgctatcgtt tttcaaccgg ttataccgct aatttaggcg
    15001 tcatcagcgc acttgccgac cataacgccg tggtgttact ggatgccgac agccacgcca
    15061 gtatttatga cgtctgttct ctcggtggcg cggaaattat tcgtttccgc cataacgatg
    15121 ctaaagatct ggaacggcgc atggttcgcc tcggtgaacg ggcgaaagag gcgattatca
    15181 tcgttgaggg catctacagc atgctcggtg acgttgcgcc gctggcggaa attgttgata
    15241 tcaaacgtcg tctcggtggt tatttgattg tcgatgaagc gcattcgttt ggcgtgttag
    15301 gcgcaacggg gcgcggactg gctgaagctg tcggcgttga agatgatgtg gatattatcg
    15361 ttggtacttt cagtaaaagt ctggcctcga ttggcggttt tgctgtcgtt tcggaagcga
    15421 tggaagtgct gcgctacggc agccgcccgt atatttttac cgcctctcct tctccgtcct
    15481 gcattgcgac cgtgcgctcg tcgttaagaa ctattgccac acaaccagag ctgcggcaaa
    15541 agctgatgga taacgccaac catctgtatg acggtctgca aaaactgggt tatgagttaa
    15601 gttcacatat tagcccggta gtgccagtaa taatcggttc gaaagaggaa ggtttacgca
    15661 tctggcgcga gcttatctcg ccgggcgtgt atgtgaatct tatcctgccg cctgccgcgc
    15721 ctgcaggcat cacgctgctg cgctgtagcg taaatgctgc ccacagtcgt gagcaaatcg
    15781 atgccatcat tcaggcattt gctactctga agcagtaatg tgagcccgcc gtggtgtttg
    15841 cgccacggcg ggtttaatgt tgtctgtcat accaatacac caggctcaac gccaccaaca
    15901 caaacgctac cggtaaaccg agcgcggtaa caggtgcgct taacgcaaat agcaatccca
    15961 gattaaccat gatttgatag ctgatccagg tgagtaagcc cacgatgacg cctacagcga
    16021 gacggctacc catcccaggc gaacgcgggg cactaaaggt aaacggcaca gccagcaaaa
    16081 tcatcgccag ggtcaggatg ggttgcccca gtttttccca cagtgcaagg tggtattcgc
    16141 tgctgggttg cccggtttcc tgcaaataat ggatgtaatg attaagctga cggacagaaa
    16201 aagtattccc cggcattgat aactcttcaa gattcatact ggtgaaggcc gattgccagg
    16261 caagattatc taatgtttcc agcgtttctt tgccattcag ccatttttta tgattcacac
    16321 catgcaggac ccacgttttg tcgtcttcaa tggtggcact gcgtgcataa atgtaggatt
    16381 caagcgagag atcgtcgcga taatgaaata tctccacgcc caccggctgg ccctgctcgt
    16441 tcagcgattt caccgtcaca aattcattac cacgcctggc ccacagcata ttgccggtaa
    16501 tgtcatcgtc ctcccccaac gcggtggcgg tcgatttgat ttgcaacgcc tgttgctgca
    16561 atggcgacgc cacccactca tctatcgcgc ctaacgaaac agtccacaat atcccggcaa
    16621 ccagcgccac cagtgcaata cggaagatag aaaaccccgt gctgcgaatg gcggtaagct
    16681 cactgttttt cgataactgc cccagtccaa caataccacc caataacgcg ataaacggcg
    16741 aaagttcgac cagtgtgcgc ggtaaggtca ttagcaccac cagcaccgcc tgagtccagc
    16801 gataaccgcc cgggctgaca ccatccagtt cgttaatcag gttgaaggtg gtaaaaagcg
    16861 gcagcaatag ccctgcagcg gcggcaaaac cgagaaagag atgacggatt aaatagcgac
    16921 tgaaaacatt catcgggaaa atttctgcag caaagagaag tcacgtgcga ccagtatcag
    16981 caggcccatt agcatgagtc cgggcactaa ccacaaacca ggaatgaggg gaatcgcgcc
    17041 gttagccacc agtgtacggc agacgtcgcc gccataaaaa atggcaataa acagcaacgt
    17101 cagtggcaat aacgttgaaa atcgcccttg ccgcggtttt acccgactta atgaaatggc
    17161 cagcagcgcc atcaacaatg cggtcaggcc acggctttgt cgccattgca gttcggcatg
    17221 atcggcagga aaggcggagc gcgccagctc cgtgaccgat ttcgctttgc gtttgacgtt
    17281 cgggctttga tccagcggat tcagatgtaa ttgcagatta cggtagatct gttcgttgtc
    17341 gtcacgcccc tgatgatcga gaagataggc agtgccgttg tgcaacataa cggtcggttt
    17401 ctcgggtgat gggtcaacca catcaaccga accggcgcgg aaaatacggg ttcgattggc
    17461 agtagaagta taaatcagcg cgtcagtcag gcgattatta tcctgatcaa ccgtctgcga
    17521 aaggatcatt cgtccgttat cgttagtgtt aaatttcttt gcccgcaact ggcgaacatc
    17581 cagctccgac tgcgactgct gctccagttg ataaatctgc gcataagccc acggacgacc
    17641 atacatcgac agaagggtga caaaaatact tagcggtatc gccagataaa gaacggcttt
    17701 gtacaagcgt cccggactgc cccccgccgc agagatagcg gtaatttccg agtcggtata
    17761 catctgccct agcgtcacgc caactgacac atacagacca acaggtaaca acatctccag
    17821 tgcaatcagt actttgtaga aaacgatatc cagcacaaca tcaagagcta atgtcccatt
    17881 tgccgcttcg gtcagataac gctgtgcgga gtagctggcg aaaataaaga tcagaaaacc
    17941 gacgataatc agcaccagac ggcgcgtacc acgcatgatg tagtgttcaa ccagcttcat
    18001 ctcactaacg ccctcaatcc tttgagtttt ttattcattg atcagtttct gcgccaacgc
    18061 aatgacatga tctacctctt ccggcgtcag gctgccgtcc tttgcccact gaatttgccc
    18121 ggctttattc agcaccagaa tggttgaact ctgctccggc aagcgccagg caacgcgtcc
    18181 ctgtccattg ccgtcaataa cgaattgcgc ccacggatag cggcgtttat ttttttcgat
    18241 tttgccaaca acaaaatagc cagtaccaaa gatggcatca tcggtgttca caatggtggt
    18301 gggctgaaaa cgatcctgcg gaaagtttgc cgcttcaacc gctttaatta acagtgagtt
    18361 ctttttcttc gccgacttac gcccggcgat atactgaata atgcggactt ttcccgctaa
    18421 ttgtgtgctg ttccactttt ggtagtgaat ttcgtcgtcg ctattcagaa gcagctcgcc
    18481 gccttcctgg atataaacag gggtaaccgt tttgccagtg acgaaattgt gtgcctgcac
    18541 acccggggct aagagcatga taatcaacgc aataatcaaa cgagatgaca tcattgctcc
    18601 ggctatagcg gaaaacgcga aatcagcagc tctaagcgct gacaaatatc gctatccagc
    18661 gccaacggtg cataatacga ctggcggttt tccatatcat ccgccaccat gtcgtcgtgt
    18721 ttatctccca tcaggccatt aatggcggcg aggaaacggg aaatctgtaa cgcctcttta
    18781 ttttcaggat caagcgatct taaggtgtcg taataagaaa cctgctggct gatgattttt
    18841 cgcacatcat gaaattccct gccggttagt cgcggtttgg caataatctc tttcagggta
    18901 tgcatttgct tttgccgata ggcaataaaa ctgcttacgc tttctaactg gctatggcgt
    18961 aatgaatagt tcaccaacga ccagaccgga gagcttaaat aaatccgtaa caggttgccg
    19021 taatacgaca caatattttt cttaccgtta cgaaagccgt cctgaaaacg ccccagaaaa
    19081 acggtcgttt tgccgaataa aaatccggcc tgatgcttct ttaaatacag ccgttgcgca
    19141 aaacgcaggt gcttgtagcg cgctcgcatg tatttaaatt gtttgcgctc atcagcagtc
    19201 agctcatcgc ctttggcttg tttgcgaact aaacgcagta attcctgacg atcaacgcca
    19261 ttgagccaga attgcaggca taacgcgtaa ttatcatgaa tgatttcggt ggtacaactt
    19321 agtgcaatag tcggcggcaa actaacctgt gcatcaacga catcatcttc ttcaatcgca
    19381 acaaatagac gctcgctcgc ctcaagcggg aaatattttc tcatcatcag gatttctctc
    19441 cggctgcgtt ataaagcaag gaatggcgct gaaaaaatgc ctttaaggtt ggaattaacg
    19501 agagcgccac gtccagacat tgcccaccga agtaagcaaa cgctgctccc agcacaccaa
    19561 acagatgaca gaacatgaac agcaatacga tgtagccgat tgaggcgatg gtttgcgcca
    19621 cgagaaaagc gcgctgtttt cccgccatca atagcaatga ctcctgcggg aagcccagca
    19681 tcgaaatcac aattgcgccc agcatcacct gaatcaggtc atacgcttcg agatacttaa
    19741 cgccaaacac cagcgaaatg agcggcttgc cgacgatcaa caccgcgagc gccaccagaa
    19801 taccgattcc gcccgccagt aaaccggact tcacacctaa caaccacggt ctggtggtgc
    19861 gcggatctaa acgcatcacc tctgggtaaa aacttttacc cagcaaaccc gctggcgttc
    19921 cggcggcgtc gaagaatgtc atggcgattt taaataaccc ggcggcagcg ggtcctaaca
    19981 cgatccccac caacactgtg ctgcacgagt tacgcgccga ccagatggag tgggcaatgt
    20041 tggttgacca gacaaaactc cacgcgcctt taatgtgtcg ggcagactca aacagattca
    20101 atttgaaggc gttatggata tttctacggc gtaattcgcg cgcggcaaac caccagtaca
    20161 tggtgccgcc aaccagattc gacacgtacc aggcaataac aaaacccaca aaaccaaagt
    20221 caaaatacca ggctgcgacg ctccccgctg cgcgcagaaa aggtttcgtc gcctgctgta
    20281 cagcaatcaa atcaaagcga tctaccgcac gcagaatgcc ggtcggcgtg gaggaagcca
    20341 ttgaaggaat gagcgtgcaa tacagcgccg ccagccaaaa gctttggtca tccagaccta
    20401 atgaatggga aaggaatggc agtaaggcaa tgccgccgac aatcgccacc gcgccgctga
    20461 cgatatccag cgagaaggaa aatgagacga cattgcgaaa ttgctgcgga ttattgttgg
    20521 tcaaggctgg cgttccgtac tgaaccacca gctgccatgt ctgaaactta ataaaatcgc
    20581 tgatcgactt ggcgtacgat tgcacaatca ccagtacacc aaacatggcg ggcgtcatcc
    20641 ctttaccggc acacgagagc gccaacagcc ccagcaaggc gctctcgaca ttactggagc
    20701 ctaaccaggc gctattgcga ataatggtgc gaaacgcgcc atctgcaaac caatgtttga
    20761 tgttaaaacc cgccaattca gcctgacctt atcgttgata gtaaaaaata tcccgccagc
    20821 cttaagttaa acttcggcgg tcagaaacga tggcaaccag agaaaccgcc ttctgtacct
    20881 gttccagcac ttcgctgtag ggcgctctgg aatcaatctc aagaattttt gtgccgttat
    20941 agccaatcgt cgacatgacg ccgattttgt cctgcagctc ggcatactca tggtcaggct
    21001 tgcgggagat ggcagtatca atatcaatcc ccaaacgaat gattaattca ggacggtatt
    21061 gcgccatttt ttggtataaa ctccgttcgc gctgcgccag aaacatgctg attttcccgg
    21121 tcgcacgttc gacgccgatc cccggtccgt cataataaaa gcccgaaatt tcagcctgcg
    21181 ggaagcgatc gctgaccacc agaacgccac tttgcgccag tcgctgaacc ttgcgtagat
    21241 tcgccattct tcgcagcgag aagcagtaca taatcaccgc cgcccatagc gccggagatt
    21301 tggttttcat gctttgggtt ttcgaggatt tggccgccaa tcgccgttcc agccagacgc
    21361 caaccaacgg caatcgtttg attttgtcgc cgtcttcgcc ggagagcagc cccagatagc
    21421 ggcgctcggt ttgccagtgt tgttgcagcg atttcaccag gtcggtggtc agcgtggatt
    21481 taccggtgcc atcacacccg accaccgcaa tcagccccgg aatgtagttg ggctgcggcg
    21541 cggttgtact attaacagtt tgtgtttgta gtgcatccat tcgttaaatc atcccctatc
    21601 cattttggtg atcaggaaag cgcggcgtga attgcgcgta gtgacgcttg cataatttca
    21661 tcggctggtt gccgaccatc cagttcaagg atcttggcgc cattgaatgt cagttgcggc
    21721 gtaacggcga ttttttcctg taacgctgcc agttggtggt caggtttacg cgcaaacgcg
    21781 gtttgttcat caatgccaag acgaatcaac aatacgggta aataagacgc catccattgg
    21841 tacagcttca actcgcgctg ccttaacatt tttatccaac cgttaccgcc cgtggttttt
    21901 gccaattgcg ggccatcaaa gcgaaaaccc ggcacttcaa cctgcgggta gcggtcggtg
    21961 atgagcagat agccttgctg gcttttacac aacattttgc gaaacttgta cgcccgccag
    22021 caggaaagca gatagataac cagcgcagta atattgccag gcggagttaa gggcttttcg
    22081 tgtacatgtg ccgctttact tcgcagataa cacccacaag gtgcgccaat aacagggagc
    22141 tgtgaaatcc attcgccaat tcgcccggac gattgcccga gataaatgtg ttctgttggc
    22201 attcttgctg ccagttcatt taccaggctt gccgtgaggg tcgatttacc tgaaccatca
    22261 catcctacaa tggcaataac acgcactggc gtggaattaa ttattgacat attacgttga
    22321 ttcacgaaaa acccggcagt aaataatgta ttgaatatta cgtggtccgt gctcagaata
    22381 tccgttcaac cttattgtga caatgactaa ttgtgacatt gacctgaatt acgctttcat
    22441 aaaaacatat taaccaaata aatattttta atggatattt aaattaaagg atatattcat
    22501 gcagtcaata acacctccat taattgccgt tattggtagc gatgcttcag gcaagtcaac
    22561 ggtgtgtgaa catcttatta ccgttgtcga aaaatatggt gctgccgaaa gagttcattt
    22621 aggaaaacag gccggaaatg tcggtcgtgc agtgacaaaa ttacctttga tgggaaaatc
    22681 cttacataaa acaattgaac gaaatcaggt gaaaacagca aaaaaattgc ctggaccagt
    22741 tccggcgctg gtaattacag cgtttgtcgc ccgtcgctta ctgcgctttc gtcatatgct
    22801 tgcctgtcgt cgtcgcgggt taattgttct aaccgaccgt tatcctcagg accaaattcc
    22861 tggcgcttac gatggtacgg tgttcccacc taacgttgaa ggtggtcgtt ttgtctcatg
    22921 gctggcaagc caggaacgta aagcgtttca ctggatggcg agccataagc ctgatctggt
    22981 catcaaactc aatgttgacc ttgaagttgc ctgtgcacgt aaacccgacc ataaacggga
    23041 atcgctggcg aggaagattg ccataacgcc acagttaacc tttggtggtg cacaactggt
    23101 tgatatcgat gccaatcagc cactggaaca ggtgttggtt gatgcagaaa aagcgattac
    23161 ggattttatg accgcgcgtg gttatcacta gtcaaaaatg gaaatgcccg atcgccagga
    23221 ccgggcattt tcaggaaggt taaatcaact gcaatgctat ccagtacagc ccaccagaaa
    23281 gaaaaattgc cgccggtaaa gtaaacaccc acgccatcag gatgctggtt accgttttac
    23341 gctgtaaccc accgccgtcc accaccatcg tccctgctac cgcagacgag aggacgtgtg
    23401 ttgtggagac aggcatccca atataactgg caaggccgat agacactgcc gccgtcattt
    23461 gtgccgccat gccttgcgca tacgtcatgc cgcgcttacc aatcttctca ccgatggtca
    23521 tcgctacacg acgccagcca atcatggtgc caatgccgag cgccagtgct accgccatga
    23581 tgatccacac cggagcgtac tcaatggtgc ttaacatatc gctgcgcagt tttttcagca
    23641 ggttctggtc ttctttactg acgcctggca gtttcgccag cttcgcggag gtatcggaga
    23701 tgcacagcat aatgcggcgc agctggctgc gctgactcac gcttaacggc tcgtaacttt
    23761 ccatattgcc tggcagcatc gttttaacgc gcgcaatcgc atcaaaggta tttgccggat
    23821 gacagtgaaa ctctgttact tgcgtgccat cagtcgatgc tgcaggcaat ggaggttcca
    23881 tcgcaatcaa cttctgcggc agttcaggat gctgttgcag gtagtgttcg aagttggtaa
    23941 cggcatcgcg ggtacgggta atttcatagc cggacgcatt catattgacg acgaagccag
    24001 caggggcaat ccccaccagt accagcatta ccaggccgat ccctttctgc ccgtcgttcg
    24061 cgccgtgcga aaacgccacg cccgcagcgg aaacaatcag cgcaatacgc gtccagaatg
    24121 gcggtttacg tttgcccttt ttctttttgc gatcttccgg aatgcggtga atacggtcac
    24181 gctttttcgt cccactccag aaacggcgca gcaggaaaat cagaccaccc gcaatgacca
    24241 ggccgacgat aggggaaaca atcagcgagg agaaaatttt ggttacttca cgcaggttta
    24301 acgcatccat caccgatgag ccggttaaca gcgcgttggt taaaccgatg ccgataatcg
    24361 caccaatcaa ggtgtgcgaa ctggaggccg gtaaaccaaa gaaccacgtt cccaggttcc
    24421 agataatcgc cgccagcagc atggaaaaga ccatcgccaa accgtgagtt gaccccatat
    24481 tcagcaacaa atcgattggt aacatatgga caatggcata ggcaacgcta agtccgccca
    24541 ataacacgcc aaaaaagtta aaaaatgccg ccatcaccac agcaagttgt ggctgcatgg
    24601 cacgagtata aataacggtt gccaccgcat tcgccgtgtc atgaaaacca ttgattgctt
    24661 cgtagaacaa cacaaatgcc agagcaagta ataacaaaag ccccgtatat atatcaaggc
    24721 caacaaataa atttagcata tattagatta cgccattttg aatttacgaa cggacgcatt
    24781 atcagtgact ttaacggcat gggcaaagtg aaatatcatt ttttaacgat aaaaagagtg
    24841 ttatttcttg tgtatcagac aataagcatt cattcaaaac ctttagaaat aatcaaaaca
    24901 caggtattcc actggttaat atattcacat atgaaatgaa taaatattgg aatatataaa
    24961 tattgaatat tttgattaat ccccgtacta attattcttc ataatcagta cggggttgct
    25021 acaacatgta ttactttttc accacaatta acggttcaat atcactctct tttttgatga
    25081 ccagtgattc atcaccgcgc aaacacgtcc caccgtagtt gccgccaacg gtgaaggtac
    25141 atacctgaat gtatttaccg tccactttcg gcaaacacca cagttgctga tagatgtttt
    25201 tctgctcggc aaatttaccg ctggttttgt ccagcacctc ttcatgatgg ctgaccaggt
    25261 cgatattgct accacagcga ccggcgatcg gtttcactgc gtaacctgtt ttcaccaatt
    25321 catcattaac agtgaaatcg gtatccagca ggtaacgatg gtgcgggaac agcgaccaga
    25381 ggatcggcag aatcgctttg ttgccgggga tcaccgtcca cagcggctca aagaccagca
    25441 cttccgggcg cagcaatacg tcgataagac gcacttcgtt ttgcggatga ccggtacgga
    25501 ttggcaccgc agcaaactca cggtcgctaa cttcacgaat ctgatcaaac gcggtttccc
    25561 acgcccaggt tttccacacg cagttaacca gtcgcccttc cccatcaatc agttgcccgg
    25621 cagcatccca gcccagttca tccagcccgc gcaagatacg cgtttcaaag cccgcctggt
    25681 gcagcgcctg ctccataaac tgcgcgtgat agttttcctc gatatctttg tcctgcatga
    25741 tatggacaaa cggacgtgca cgactgtgtt tccaggcacc agccagttcg ttaatcagcc
    25801 cttccgccgg attgaagccg ttgcctttat agccctgctc cgcccaacgt tcgaggatca
    25861 agcccgcttc ggtatgacag gaggcggagt cggcgttgta ttcgtaaacc ttcaggccac
    25921 gctcatccat gcagaaatcc atacgaccag tgatcatatg gtgacggcga cgctgccagg
    25981 agagacgcaa acgtggccag aggattttcg gaatgtcgaa cagcgccagc aggttgtcat
    26041 ctttcagcac cttgtcggtt gcgtgaagat acatcagatg cagctcgttg gtggctttaa
    26101 ttagctcctg ctcggcactc tcggtaatgg tgtagtaatg atacggatcc tgattgatca
    26161 cctgaccgtt ggcctgcaca taggcgtttt gtagcggatc tttttcatcc agccatttac
    26221 cgtcaaactg gcctttgttt tccagacgcg ctccactgat tttcagcagc tcgcctgcaa
    26281 tttccggctg cggtaagctg tattcagtat cttccgtctg gatcatccag cccagaatgg
    26341 tggtgtcatc aaaagtgtct ttaagggtat agcagccgtt ttcgaccacc atctccagct
    26401 cgcgcgtcca ctgttgccct tgcggcaacg gggaatgaat cacgttctgt tccgcaatac
    26461 ggactttgtt gccatgcaat tgggtaatga tggcgacatg gccagtgtct ttaaattcac
    26521 cgcctttatc ccagataaga agcgcacccg cgaccggcgc acgcggcgag ccgttaggaa
    26581 atgcctgcaa tggcaggatg ttgtcattaa ccacttctcg caggaagcgc agcgagaaaa
    26641 tctcccacgc catacccacg tcagtaaaga ccacaccgta attcagaaag agaaaacggc
    26701 gagcaaattc aacgcattgc cacttgtggc ccatatattc gtcgtcgata tagctacgga
    26761 atacggcgtc atcttcgtat tcctgcggat cgagagaact gtaatctgaa gagtagattg
    26821 ctaccccacc tggggcgtag cccaataatg tcccgaacgg ggcatcctgg ctggtcgttc
    26881 ctttgctcat cactttacct ttaacaatac aacctaagca gttggcgtaa ttgttgtgct
    26941 ctgattacct gctcagcgac attaaccgga cagaggtcag actaatcata cacctcatcg
    27001 cagcggctgg cgcaaagggt taaaaaattc acattctgta cagcaagtga cctgctacac
    27061 tgcttcaaca ctaccactca gaaggcaact cactatgaca gacaatactt atcagcccgc
    27121 gaaagtctgg acgtgggata aatccgctgg cggcgcgttc gccaatatca atcgcccgtt
    27181 ttctggtccg acgcatgaaa aaacgctgcc cgttggcaaa catccattgc aactttattc
    27241 gctgggaacg ccgaacggtc agaaagtaac gattatgctt gaggagctac tggcgctggg
    27301 cgttactggt gcagagtacg acgcctggct gattcgtatt ggcgatggcg atcaattctc
    27361 cagcggcttt gtcgaagtga acccaaactc gaagatcccg gcgctgcgcg atcatacgca
    27421 taatccgccg atccgcgtgt ttgaatctgg ttcgatcctg ctttatctgg cggagaaatt
    27481 tggctacttc ctgccgcagg atctggcaaa acggactgaa acgttgaact ggctgttctg
    27541 gttacagggt gccgcgccgt tcctcggcgg tggttttggt cacttttacc attacgcgcc
    27601 ggtgaaaatt gagtacgcca tcaaccgctt taccatggaa gccaagcgcc tgctcgacgt
    27661 gctggataag caactggcgc agcataagtt tgttgcgggc gatgagtaca ccattgcgga
    27721 tatggcgatt tggccgtggt ttggcaacgt ggtgctcggc ggcgtgtatg atgccgcaga
    27781 gtttctggat gcgggaagtt ataaacatgt gcaacgctgg gcgaaagaag tgggcgaacg
    27841 tccggcagtg aaacgtgggc gtattgttaa ccgcaccaac gggccgctga acgagcagtt
    27901 gcatgagcgc catgacgcca gtgattttga gacaaataca gaagataagc gtcaggggta
    27961 agggttggtg ttcgtcgcag caagccatcc aggccggata aggcgttcac gccgcatccg
    28021 gcaatcgtgc acaatgcctg atgcgacgct gccgcgtctt atcaggccta caaaagctaa
    28081 tcatcacgcg ctggtaatgt cgtaatccat ttggcgcagt gcgtctaatg tggctttggc
    28141 ttcatcttcg tcgatgatgc tcatggcaaa tccgacgtgt accagcaccc actggcccag
    28201 cagatcggca gggttacctt cgcaaatcag ggcgatattc acgtcgcgct tgataccaca
    28261 tacttcaacc tgcgcaagct ggtgaatatc ttcaccgaca gccagcacct ggcctggaac
    28321 gccaatacac attgttaact ccggttattt cgattgccgg acaaacgcga aaaagaacct
    28381 ttagcgggtg ttccctctca ccctaaccct ctccccggag gggcgagggg accgtccgtg
    28441 cgctttttag gcgttgtcct cagtcttatt caacttcaat acttttgacg atcagcgaat
    28501 cgccggtatc gacacgcaac cgctcgccgt gacagagcgg acactgcgca tcgtgctgat
    28561 gaatctccac cacctggctg caatcccagc accaggcctg ggcgggttta tagacgatat
    28621 gtaaatcgca cccttgcgcc accgttccgt ggcagacaat ttcaaaacta aaacggacgg
    28681 cgctctcctc aacgcaggag agcgcgccaa tttccagcca cacggcggtg acgcgcttaa
    28741 catcgtgctg ctccgcctgc cgttggataa tttcaacggc gctctggcaa agcgacaact
    28801 catgcatttt cgcccctcct gcggccaaac agcagggcgc gacgccctgc gtgtggtaca
    28861 tccggattgg tgactggcaa cgaaaggatc attcgtgcgc agtcatccgt caggcgttgg
    28921 ccttcttcaa tcgacatgct gtgcgaaagc ggcgacatca gcgaacagga gaggtattgc
    28981 gaaacaccgt ccagttcgcc aacggtaaag gtcatggtgc catacggcag ttgcagacca
    29041 attttttcac tcactttgcg cagcggccag agttgatccg ggccggggaa gataactgca
    29101 ctcagcatcc acggggtgat cacacacccc gtccactgac cttcgaacag cgtaaaatca
    29161 gaaacataca ccggcattga aggatgcaga aaagagagat cgtgcatcga acgccgggca
    29221 atttcttcaa acgctgcctg tacttgcgcc ttcggggagg tctggaaacc tgctatctct
    29281 tcagtcatga atcgcctccc gtgggatggc ttccacgcct gattcgcgca gcgcagccag
    29341 aacctgctca agcgcaggtt caatcattgc ttcaaccgtc ggcgttaagc cgatgtgtgg
    29401 ctccagcgat tccgggatca cgccgaccag ggtcagcttt ttcggaaact caccggtgaa
    29461 gcgcagggcc gacaatacgt cggccaggcc aagctgatgc ggagagattt tgttggtaaa
    29521 caacgccgga acttcttcat cccgcaggat catcatcgtt cccggagtgt tctttttcga
    29581 cacaatggca tccgcaataa tcagatgatc gcgatttgcc atgtcgccaa gcagctccat
    29641 tcccgccgtg ccgccatcga ggatctcaac ataatccggc agaatgtatc gttgctctaa
    29701 cgcttcgaca atccgcacgc cgatggcttc atcggtcagc aaaatattgc cgacccctaa
    29761 gactaaaata cgcattacag aaccttcact gaaaccactt cgttgccgtc ggcatccact
    29821 acgtgtaccg cacaggccat gcacgggtcg aaggagtgaa tggtacgcac cacttccagc
    29881 ggtttattcg gatcggcaac cggtgtaccc accagcgact gctcgtaagg accgacgtca
    29941 tcattgaagt tacgcggacc agagttccag gttgatggaa caaccgcctg gtagttgctg
    30001 atgataccgt ctttaataac catccagtga gagagcatac cgcgcggcgc ttcgaggaag
    30061 ccaacacctt tgaactcacc cgttgccgga atgttcggct tcacaaaggt ggtgtgatcg
    30121 cctttgccga tattggtgat cagtgcactg tattggtttt gcaggatatc ctgcaattca
    30181 cagcagtgaa cggtacgacc aataatacgg cccagcgtgg agtgcagctg tgccacttcc
    30241 agcgtgttgc cagtcagttt ctgataaatc gcaacgattt cattcagttt gttctgggta
    30301 gattcgcgac ctgccgccag tttcaccagc atattagcca gcggacccac ttctaccgtt
    30361 ttgccgtaga aagtcggtga tttcacccag gaatatttcc cgtcgtcaga ccaaccatca
    30421 taagccggaa tggtggtacc ttcccacggc gcctgtggcg cttcgtcttt ataccaggag
    30481 tgcttcgcgc tttcctgaat gcctttgatc aggtattcat cggaatgaga agtgatcgga
    30541 cgatacgagg acagatccgc attctcaatg tagcctcccg ggaacaggaa gctgccgttt
    30601 ttgctgtcgg tcgggaattc cggcacgctc aggtagttca ccgcaccttt accgcgcgtc
    30661 agccattccg ggtagaacgc ggcgataacc gcggtatcaa ccttataaac ctgctcaaca
    30721 aagtcgctca gtttgtcgat gaaagacttg atgtacatca ggcgctcaag gttcaacacg
    30781 cccaacccgt cgaggttgat tgggttcgcg acaccaccca cagccaggtt ctgaatgtgc
    30841 ggcgttttac cgcccagcag cgccacgacg cggttagcgt cacgctggca ctccagcgcc
    30901 tgcaggtagt gcgctaccgc aatcaggttc acttccggcg gcagtttcat cgccgggtga
    30961 ccccagtagc cattggcgaa aatacccaac tgaccgctgg caaccagatc tttgatcttg
    31021 ttctgaactt tagtgaactc ttccggactg ttcagatgcc aggtcgaaac gcctttcagc
    31081 atttcggagg ctttggttgg gtcagcttgc agtgcagaag tgatgtccac ccagtccagc
    31141 gccgaaagct gatagaaatg aacgatatgg tcatgcgtgg tgtgcgcagc cagaatgatg
    31201 ttacggatgt attgcgcgtt aaccggaacg tcgatattca gcgcactttc tgccgcacga
    31261 acggaagaca gcgcgtgagt ggtagtacat acgccacaga tacgttgcac aatcatccat
    31321 gcatcgcgcg gatcgcggtt tttcacgatc tcttccatgc cgcgccacat ggtaccggaa
    31381 gcccatgctt tcgaaacgac gccattttcg atttcgcaat cgatgcgtaa atgcccctca
    31441 atacgggtta ccggatcaat agtaattctc tggctcatgc tttgctcgcc tcatgacgat
    31501 tatgatcgtt ttgttttaaa ggaggaagta tcggcagtag acgaatgagt acgatgtaag
    31561 cgcaaatctc aatagccaca aaaccaatag aaatcaacag ttcttcccag gtcgggaagt
    31621 aggcgtaacc gccgcccggg ttgaatgcca ccaacgaata ggtcagacgc caggttgcac
    31681 aacctaacag tgcgctcagt gctgacagga acagcatacg ggaatcatta cgcagcttcg
    31741 ccacacgcag aacgaccagc gggaagagca tcagcaggac ttcaatccag aacatcacgg
    31801 agtagaagtc accggcaaac gctaacgaca gcttgtcgcg atagataagc tcgccaaagc
    31861 gcagcccgat gaaaatcgcc agcaacacac tgatggtgtt ggtcagcttg acgaacaggc
    31921 tcttttcatc cggaccgttg ccacgcagac ccgcctgcac cagtgaacct tcaaagatga
    31981 caatcgagaa gcccatgatg aacgccgtca gcagcgagaa cagcggcaac atttcatagc
    32041 tctgccagag cggatgcacc ttgtagcccg ccgagatcat cagcgacccc attgaagact
    32101 ggtgcatggt cggcagcagc gcaccgagcg cgatgatgaa gaacatcacc ttgttcagac
    32161 gttgtagcga caccttccag cccagacgtt caaacagtgc cggagcaaac tccagagcca
    32221 tcacgccgat gtagatggtc atacagaccg ccgtctcgaa cagtaccgag ttcacgttga
    32281 agtgacccgg aatgtagaag tacggcaggt tccagtagcg acccacgtcg atagtgatcg
    32341 acaagccacc cagtgagtaa ccaaacagac tcgccaacag cgccggacgc accagcggat
    32401 ggtattgccc acggttaaag acgtataccg cccacgccag cgcccagccg ccacaggcaa
    32461 agccggtgcc aatcagcagg tcaaacgcga tccacacgcc ccacgggaag ccgccgttca
    32521 ggtcagagac agagcccaga ccgaacacca gacgcttcac aatcaggagc atacagatga
    32581 cgattaacgg tccaaaaatc atgaccggtt tactgatgat tttgccgccc agcggttgtg
    32641 gatcatgact catgatcgtc tcctccgtcg tgatggtcgt ttttggtgtt gcgacgaacc
    32701 agcacggtta agcccgccag cacagccagt ggtagcatca tgcctttata cagggtgtgt
    32761 tgaatatttt cggaacgcgc accggtagaa agatcgtcca gtttcggcag gtcgagattt
    32821 tcataaggca cacccgtcag taccagaacc tgagtaccgc cgccctcttt ctcgccgtac
    32881 agatgcggat aatatttcgg caccgtatgc aggtaagtgt cgccagattt cagcgtctga
    32941 cgcggatagt ggtattcgct gccaggcttc agcgccagac gttttttcgc ctccgccatc
    33001 agctcttcac gcgtaccgaa aatcaccgcg cccgccgggc acacttctac gcagccaggc
    33061 agaccgcctt tatcgagacg ttccacacct ttctggttgc acagctcgca cttatgcagc
    33121 gcaccaaacg ggttgttgta gtcgtacttc ggcacgttgt acggacaggc gaccatgcag
    33181 taacggcaac cggtgcacac gtctttgtcg taatggacaa tgccggtttt cggatctttt
    33241 ttcagtgcag agaccgggca cacagagaca cagttcggat cgacgcagtg catacactgt
    33301 ttcttaatgt acgcgtagcc gttctcctcc tggtctttgt tgacccctgt gccgctggtc
    33361 cacacctgaa tgatgttatt ggtatacggc gacagtttgt cgttgttcga ccaggtctgt
    33421 tccccttgcg ggttacgttc agggaagttg atatcctgac acttggtgac gcaagcctgg
    33481 cagcctacgc acaaggtcga gtcgtacaac atccccagcg atcccggaat tggcgggcgg
    33541 ttttcagcag ccgcatgact gacagacggc agcgcgcccg tcagcaatgc cccgcaggag
    33601 gctgctttaa taaaattacg tctgttcacg gttattctcc ccgtgagtca gcgttatctt
    33661 tcttttgctg acgacccagt tcacgcaccg ccatcacgct gacaccggca accagcccaa
    33721 ccacaccgcc gagcaaacca atagcgcctg cagagacgtt gccgccctct ttagcgttaa
    33781 catccggttt ctgtgaacgc ggagtctgat tttcgacgtt ggcaagctga tggatgcctt
    33841 tatggaagcc gatacctttt tcgttacagc catagcacgg gtgaccaatc gccaccggcc
    33901 acacaccgcc aacatcgcag aattgcagcg ttgagcagtt gccgtaagtt tctggccctt
    33961 tacagccgag gtggtacaag caccagcctt cgcggtggcc ttcatcaccg aactctttgg
    34021 caaaacgacc agcatcgaag tgcgggcgac gttcgcagtg ttcgtgaatc agacggccat
    34081 aggcgaaggt cggacggttt ttgtcatcca gtttcggcgg tttgccgtaa gtgatgatgt
    34141 gcgcaacggt cgcgaggaag ttgtgcgggt tcggcgggca gcccggaata ttgataacgg
    34201 ttttgcctgg cagaacttct tgcaagctga ctgcgccagt tgggttaact ccagctgcgg
    34261 caacaccgcc ccacgcggag caggaaccaa tagcgataat ggctgctgag ccttccgccg
    34321 ctttgcggat gtgatccaca atcggctcac cggcaaccat gcaataaata ccgttatctt
    34381 ttaatgggat ggaaccatcc accactaaca catactgccc tttgtacttc tcgagagcgt
    34441 tatgtttgtt ctcttcgacc tgatgaccga aggcggcgga aagcacttcg tgatattcca
    34501 gagagatagt ctccagcacg aggttttcta ccgttggatg cgttgcacga agcagagatt
    34561 ccgtacaacc ggtgcactcc tgcgcgccaa tccagataac tggcggacgc tgcgggttag
    34621 taaccgattc ggccatctct gcagcggctt tgctacttaa ccccatggtg gcggctaatg
    34681 ctgcacaaag cttcatgaaa tcacgacggt taatgccgtg agaatggatg agggtgttat
    34741 ctccagtcat ttatagttat tccgttgcga gaacctggca tatattttgc ctcaatcgca
    34801 aaatcaataa tgcgatcgat gcgccattta ccacacattt attatggtta tcgatatcat
    34861 gatactgcgg cggggcgcta aaacgaaggg aagaagcatt agtgtaatta attaaagcaa
    34921 gataatacgt atgtttgatc aattttcgag gcgataaccg gccataaaaa agcgcaacgt
    34981 agaacaggaa ttattctcac gaaagaaatc cattaccatc tctttatcca gtccatagcg
    35041 gcgcgtcaga atacccgctt cccaggcgct aagctggcga tctccggtct tttcgtacat
    35101 cctgtgtgag tagcctaata caaaaccgcg tttataatcg gcacagaatt ttgtcacatt
    35161 aaccgcactg tcggcatgcg ttgcttttaa tcccgccatt aagcctttac caaaatggtt
    35221 attcatcgtt cacctcattc attatatatc gccatatata acgatatttt aggcaaagaa
    35281 cgacttttgc gcacgctatt ttttcatact tgtttaagac gtaatcccgt aagagataaa
    35341 cctatcagga gagatattta tcgaaccatt tcagtgtcct ttgccaggca agatcggcgg
    35401 cagatttgtc ataacggggc gtggaatcat tatggaatcc gtgattaacc cccggataga
    35461 tataagcctc ataaacctta ttattgtctt tcaacgccgc ctcgtaagga ggccagccct
    35521 cgttgattcg ggtgtccagt tccgcgaagt ggagtagtaa aggcgcttca atcttcgcca
    35581 catcggcagt gggtgcctga cgaccataaa acggcaccgc gcaggccagt tccggatacg
    35641 cgacagccgc cgcgttcgat acgccaccgc catagcaaaa tccggtaata cccactttac
    35701 ctgtcgcttg cggatagcgt tgcataaact caattgcggc aaagaaatca ttcatcagtt
    35761 tggttggatc aacctgctgt tgcagctcac gacctttatc atcatttccc ggataacctc
    35821 caacggaact taatccgtca ggtgccaggg cgatataccc cgccttcgcc actcgccgtg
    35881 ccacatcttc gatatacgga ttcagtccac gattctcatg caccaccccc acggctggcg
    35941 ttttgccgct catttttgcg ggtttcacca gataaccccg tacctcgccg tgaccatttg
    36001 gcgaaggata cgtgatgtac tcagcaacaa tctccgggtc ggtaaactct acctgagtcg
    36061 ccagcgcata atttggcttg agcaaatcaa acaacgctaa tgccgtcatc ccgcccaccg
    36121 catacttcgc cgcaaggttg aggaactcac gtttcgaaat tttcccgtga acgtaatagt
    36181 cgtagtaatc caacaactct tgtgggaaat ctttggcggt taaacgcggc atcgttgcat
    36241 tcctcaactg gtgttttttt aagcaaagca taagcgcgtg tttttgccca gtttttcgtc
    36301 actctgtgag ccagactacg ggatacgcgc tggcgaatcg ctaaactaga aacattgttt
    36361 cgaaattgaa cggtggaaag gagaggtcat ggtctggtta gcgaatcccg aacgttacgg
    36421 gcagatgcag taccgctatt gcggaaaaag tggtttacgc ctgcccgcat tatcgctcgg
    36481 tttatggcac aatttcggtc acgttaacgc gctggaatca cagcgtgcaa tcctgcgtaa
    36541 agcgtttgat ttaggcatta cgcactttga tttagccaac aattacgggc cgcctccagg
    36601 aagcgcagaa gagaactttg gtcgcctgct gcgcgaggat tttgctgctt atcgcgatga
    36661 actgattatc tctaccaagg ctggctacga tatgtggccc ggcccgtacg gctctggcgg
    36721 ttcacgtaaa tacctgctcg ccagcctcga ccaaagcctg aagcgtatgg ggcttgagta
    36781 tgtcgatatc ttttactctc atcgcgtcga tgaaaatacg ccgatggaag aaaccgcctc
    36841 tgcgctggct catgcggtac aaagcggtaa ggcgctgtat gtcgggatct cctcttactc
    36901 gccagagcgg acgcaaaaaa tggtcgagtt gctgcacgag tggaaaattc cgcttttaat
    36961 tcatcaacct tcgtacaatt tactgaaccg ctgggtggat aaaagcggcc tgctggatac
    37021 cctgcaaaat aacggcgtgg gctgtattgc ctttactcct ctggctcagg gattgctgac
    37081 cggaaaatat ctcaacggca ttccgcaaga ttcacggatg catcgtgaag ggaataaagt
    37141 tcgtggtctg acgccgaaaa tgcttaccga agccaacctc aacagcctgc gcttattgaa
    37201 tgaaatggca cagcagcgtg gacaatcaat ggcgcaaatg gcgttaagct ggttgctgaa
    37261 agatgatcgc gtgacgtcgg tattgattgg tgccagccgc gcggagcaac tagaggagaa
    37321 cgtgcaggcg ctgaataatc tgacatttag caccgaggag ctggcgcaga ttgatcagca
    37381 tatcgccgat ggcgagctga atctgtggca ggcgtcgtcc gataaatgac ctgttaataa
    37441 cgggcgcgcg aaccgcgccc gaatcagatc agtgattatg acgagtcagt cggtcaagat
    37501 agcccatcac aaatgcagaa agcacaaacg tcagatggat aatgacgtac cacatcagtt
    37561 tgttatccgg gacatttttc gcatccataa agacgcgcag taagtgaatg gaagaaattg
    37621 ccacaatcga cgctgctact ttgtttttca gcgacgttgc gtccattttc cccagccact
    37681 tcagcttctc tttgttctcg gagatatcca gctgtgagac gaaattctca taaccggaaa
    37741 acatcaccat caccagcaaa ccgccaacca gcgtcatatc caccagcgac agcaacacga
    37801 ggatcaaatc tgattccgcc atcgagaaga tattcggcag tacgtgaata atctcctgga
    37861 agaacttcag cgccagggca actaacgcca gcgaaaggcc aaagtacacg ggggcaagca
    37921 gccagcgaga agcatacatt gcattttcaa gaaaacgttc catacattcc tgtcgtgtag
    37981 ttaaaacggg caacagtata tcgcaattga gtaaacagca cgcaacagca aatcaacata
    38041 tacgttaaac ctttgtcgca taattctgag gtttagggat cttcctggtt cccttatcaa
    38101 cgcatcgaga gcgtctaccc ttaaaggacg ttgaacctga agggagaaaa acgatgtctc
    38161 atttaaaaga cccgaccacg cagtattaca ctggtgaata tcccaaacag aaacaaccga
    38221 cgccaggcat ccaggcgaag atgacaccga taccggattg cggcgagaaa acctatgttg
    38281 gtagcggtcg cctgaaagat cgtaaagcac tggtgacagg gggcgattcc ggaataggtc
    38341 gcgctgccgc catcgcttac gcgcgtgaag gggctgacgt ggcgatcagt tatcttcccg
    38401 tggaagaaga agacgctcag gatgtgaaaa agatcattga agaatgcgga cgcaaagccg
    38461 ttctgctgcc aggcgattta agcgatgaga aattcgcccg ttcgctggtt cacgaagcgc
    38521 acaaggcgtt aggcgggctg gatattatgg cgctggtcgc cgggaaacag gttgccattc
    38581 cggatattgc agacctcacc agcgaacagt ttcaaaagac ctttgccatt aacgttttcg
    38641 cgctgttctg gctaacccag gaagcgatcc ccctgctacc gaaaggtgca agtgtcatca
    38701 ccacttcgtc aatccaggca taccagccaa gtccgcattt actggactat gcggctacga
    38761 aggcggcgat tctgaactac agccgtggcc tggcaaaaca ggtcgcggag aaaggtattc
    38821 gggtgaatat tgtcgcgcca ggccctatct ggacggcact gcaaatttcc ggcggacaaa
    38881 cgcaggataa gatcccgcag tttggtcaga aaacgccgat gaagcgtgcg gggcaaccgg
    38941 cggaactggc ccctgtatat gtttatctgg caagtcagga gtcgagctac gtcaccgcag
    39001 aagtgcacgg cgtgtgcggc ggcgagcatt taggttaaaa aaatgcccgg ttgtgaaaag
    39061 caaccgggca tcattgtgaa ttcactctta cctgatgtgg cgaaaacgcc ttatccgcct
    39121 acaaacgcat gaagattcaa catgttgcaa tagattttgt aggcctgata agcgtagcgc
    39181 atcaggcatt gagcacctaa tgccggatgc gtcgtgaacg ccttatccgg cctacaaacg
    39241 cacgaagatt caacatgttg caatagattt tgtaggcctg atgagcgtag cgcatcaggc
    39301 aattctactt tacttcgctt tggcggtttc ttcgccgacc agacctatct tcaggtaacc
    39361 cgcctgatgc agcgtatcca ttaccttcat caacgtctcg taatcgacgg ttttgtccgc
    39421 tcggaagaag atggtggtgt ctttcttgcc ttcggttaac gcattcaacg ccgtaatcat
    39481 tgtttcatcg gtgaccggat cgttaccgat aaacatcgag ttgtctgcct tcaccgacag
    39541 ataaaccggt ttttccgggc gcggctgcgg cgtgctggta gaagcaggca agttcacctt
    39601 cacatctacc gtcgctaacg gtgccgccac cataaagata atcagcagaa ccaacatcac
    39661 gtcgataaac ggcgtcacgt tgatatcatg catttcgccg ttatcgtcga ggttttcgtt
    39721 aagatgcatt gccatcggac attatcctgc gcgtaatttt tgtgcgacac gaaccggatg
    39781 cgcagcggcg ctggcttcca gatccaggtc acggctttgc agcaacaata cctgcgctgc
    39841 aacatcaccc agcatcgctt taaagccgcc aatctggcgt gcaaatacgt tatagataac
    39901 gaccgcagga atcgctgcca cgaggccgat tgccgttgct aacagagctt ctgcgatacc
    39961 cggcgcaacg actgccaggt tagtggtctg cgtttgcgcg ataccaataa agctgttcat
    40021 gatgccccag accgtaccaa acagaccaac gaacggagaa atcgcgccga tggttgccag
    40081 atagccgtta ccgcggccca tttgacgacc cactgcggcg acccgacgct ccagacggaa
    40141 gctggtacgt tctttaatac cttcgttatc gtcgctgcct tctgacagtt ccagctcatt
    40201 ctgcgcttca ttgagcaaat gcaggcttaa gcttttgcta ccaaaatcag cggcgatatc
    40261 gttggcctgg tttaaggaac gtgcttcagc cagcagttgc tgctcgcgct taaggcgacg
    40321 cttctgattg aagaactcta cgctcttact gaagaagatt gcccaggtga ctacagaggc
    40381 caaaataagc ccaatcatca cgcacttaac gacaatatcg gcgtactgat acatacccca
    40441 gacggaaagg tccgtctgca ttaaattatt acccactgtg tatctccagg acgcaagtca
    40501 caaaatctgc gcataataat atcaaaacga cgtcgaattg atagtcgttc tcattactat
    40561 ttgcatactg ccgtaccttt gctttctttt ccttgcgttt acgcagtaaa aaagtcacca
    40621 gcacgccatt tgcgaaaatt ttctgcttta tgccaattct tcaggatgcg cccgcgaata
    40681 ttcatgctag tttagacatc cagacgtata aaaacaggaa tcccgacatg gcggacaaaa
    40741 agcttgatac tcaactggtg aatgcaggac gcagcaaaaa atacactctt ggcgcggtaa
    40801 atagcgtgat tcagcgcgct tcttcgctgg tatttgagag tatggaagcc aaaaagcacg
    40861 cgacgcgcaa tcgcgccaat ggcgagttgt tctatggacg gcgcggaacg ttaacccatt
    40921 tttccttaca acaagcgatg tgtgaactgg aaggtggcgc aggctgcgcg ctatttccct
    40981 gcggggcggc agcggttgct aattcgattc ttgcttttgt cgaacagggc gatcatgtgt
    41041 tgatgaccaa caccgcctat gaaccgagtc aggatttctg tagcaaaatc ctcagcaaac
    41101 tgggcgtaac gacatcgtgg tttgatccgc tgattggtgc cgatatcgtt aagcatctgc
    41161 agccaaacac taaaatcgtg tttctggaat cgccgggttc catcactatg gaagtccacg
    41221 acgttccggc gattgttgcc gccgtgcgca gtgtggtgcc ggatgccatc attatgatcg
    41281 acaacacctg ggcagccggt gtgctgttta aggcgctgga ttttggcatc gatgtttcta
    41341 ttcaggccgc caccaaatat ctggttgggc attcagatgc gatgattggc actgccgtgt
    41401 gcaatgcccg ttgctgggag cagctacggg aaaatgccta tctgatgggc cagatggtcg
    41461 atgccgatac cgcctatata accagccgtg gcctgcgcac attaggtgtg cgtttgcgtc
    41521 aacatcatga aagcagtctg aaagtggccg aatggctggc agaacatccg caagttgcgc
    41581 gagttaacca ccctgccctg cctggcagta aaggacacga attctggaaa cgagacttta
    41641 caggcagcag cgggctattt tcctttgtga ttaagaaaaa actcaatgat gaagagctgg
    41701 cgaactatct ggataacttc agtttattca gcatggccta ctcgtggggc gggtatgaat
    41761 cgttgatcct ggcaaatcaa ccagaacata tcgccgccat tcgcccacaa ggcgagatcg
    41821 attttagcgg gaccttgatt cgcctgcata ttggtctgga agatgtcgac gatctgattg
    41881 ccgatctgga cgccggtttt gcacggattg tataacattg ccacttttgg acaattttgc
    41941 agacatttta ttgtgaaaag tcttaaattg ttgcgtccgg gatcaaggcg tcccggacga
    42001 ttcaggagta caataggcag ataaaggctt aaacgctgtt ccacaggaaa gtccatggct
    42061 gttattcaag atatcatcgc tgcgctctgg caacacgact ttgccgcgct ggcggatcct
    42121 catattgtta gcgttgttta ctttgtcatg tttgccacgc tgtttttaga aaacggcctg
    42181 ctgcccgcct catttttgcc aggcgacagc ttgttgatac tggcaggcgc attgattgcc
    42241 caaggggtta tggattttct gcctacgatt gcgattctga ccgccgcagc aagtctgggc
    42301 tgctggctaa gttatattca ggggagctgg ttagggaata ccaaaacggt gaaaggctgg
    42361 ctggcacagc ttcctgctaa atatcaccag cgcgccacct gcatgtttga ccgccacggt
    42421 ctgctggcgc tgctggctgg acgttttctt gcatttgtcc gtacgctgct gccaaccatg
    42481 gcgggaattt ccggtctgcc aaaccgccgc ttccagtttt tcaactggtt aagtggattg
    42541 ctgtgggtca gcgtggtaac cagttttggc tatgccttaa gtatgattcc gttcgttaaa
    42601 cgccatgaag atcaggtaat gacgttcctg atgatcctgc caattgcctt gttaaccgct
    42661 ggcttgttag gcacgctgtt tgtggtgatt aaaaaaaaat actgtaacgc ctgacgattt
    42721 tccccgttcc cggctgctgt accgggaacg tatttaattc ccctgcatcg cccgcattct
    42781 tgccgcatct tcccccggcg tcacaccgaa gtaacgttta aactcacggc taaattgcga
    42841 tgcgctttca tagccgacgc gcatcgctgc tgcgctggcc ttcatgccgt catggatgat
    42901 catcatccgc gccttatgca gacggtaatt cttcaaatac tgcaacggcg aggtgctggt
    42961 gacagactta aaattatggt ggaacgccga tacgctcatg ttggcttctg ccgccagttg
    43021 ctcgacgctc aggttttcgg tgtatttatt ctcaattcgt ttcagcacgc ggctaatcag
    43081 actgaagtga gtctggcgac tgaccagcgc cagtaacgcg ccgccgcaag gtccggtcag
    43141 cacgtagtac agaatttcgc ggatgatctg tttgccgaga atacgcgcat ccagtggtcg
    43201 ctccatcacg tcgagtaacc gctccgccgc gcataaaatc tcttctgata acgtggcgga
    43261 gttaatcccg ctggctgcca tcgacggctg gaaatgctca tcttcgccaa tgtccatcaa
    43321 cagttcctgt aactgcaaaa tatcgacatt gagacgcaac cctgccagcg gcacctctga
    43381 cgtggcatag gtttcgcact caaacggcaa cggcaccgtc agcagcaggt attcattggc
    43441 atcataacga aacacgcgtt cattgatata accgatttta tgcccggaaa agagaattat
    43501 gatgccaggc tcgtacatca ccggtgtacg tgcgaaaggc gtctcgccat acaacaaacg
    43561 cacatcgggc aacagttctg acaaactatt ttctttattt ttcagtttat taactttatc
    43621 cgccagcaag cgacaaatct cttcacgttt catatcgcgt aatttcttag gaataatgcg
    43681 gcaatttgat tgtgcgcaat tttgtagcat ttctccagca ctctggagaa ataggcaaga
    43741 cattggcaga aatgagcatt gagagccagg gcgctggcga tcacaatgaa aaacatcagg
    43801 cagatcgttc tctgccctca tattggccca gcaaagggag caagtaatga acaactttaa
    43861 tctgcacacc ccaacccgca ttctgtttgg taaaggcgca atcgctggtt tacgcgaaca
    43921 aattcctcac gatgctcgcg tattgattac ctacggtggc ggcagcgtga aaaaaaccgg
    43981 cgttctcgat caagttctgg atgccctgaa aggcatggac gtgctggaat ttggcggtat
    44041 tgagccaaac ccggcttatg aaacgctgat gaacgccgtg aaactggttc gcgaacagaa
    44101 agtgactttc ctgctggcgg ttggcggcgg ttctgtactg gacggcacca aatttatcgc
    44161 cgcagcggct aactatccgg aaaatatcga tccgtggcac attctgcaaa cgggcggtaa
    44221 agagattaaa agcgccatcc cgatgggctg tgtgctgacg ctgccagcaa ccggttcaga
    44281 atccaacgca ggcgcggtga tctcccgtaa aaccacaggc gacaagcagg cgttccattc
    44341 tgcacatgtt cagccggtat ttgccgtgct cgatccggtt tatacctaca ccctgccgcc
    44401 gcgtcaggtg gctaacggcg tagtggacgc ctttgtacac accgtggaac agtatgttac
    44461 caaaccggtt gatgccaaaa ttcaggaccg tttcgcagaa ggcattttgc tgacgctaat
    44521 cgaagatggt ccgaaagccc tgaaagagcc agaaaactac gatgtgcgcg ccaacgtcat
    44581 gtgggcggcg actcaggcgc tgaacggttt gattggcact ggcgtaccgc aggactgggc
    44641 aacgcatatg ctgggccacg aactgactgc gatgcacggt ctggatcacg cgcaaacact
    44701 ggctatcgtc ctgcctgcac tgtggaatga aaaacgcgag accaagcgcg ctaagctgct
    44761 gcaatatgct gaacgcgtct ggaacatcac tgaaggttcc gacgatgagc gtattgacgc
    44821 cgcgattgcc gcaacccgca atttctttga gcaattaggc gtgccgaccc acctctccga
    44881 ctacggtctg gacggcagct ccatcccggc tttgctgaaa aaactggaag agcacggcat
    44941 gacccaactg ggcgaaaatc atgacattac gttggatgtc agccgccgta tatacgaagc
    45001 cgcccgctaa gctttttacg cctcaaactt tcgttttcgg gcatttcgtc cagacttaag
    45061 ttcacaacac ctcaccggag cctgctccgg tgagttcata taaaggagga acgtatggct
    45121 aatccaaccg ttattaagct acaggatggc aatgtcatgc cccagctggg actgggcgtc
    45181 tggcaagcaa gtaatgagga agtaatcacc gccattcaaa aagcgttaga agtgggttat
    45241 cgctcgattg ataccgccgc ggcctacaag aacgaagaag gtgtcggcaa agccctgaaa
    45301 aatgcctcag tcaacagaga agaactgttc atcaccacta agctgtggaa cgacgaccac
    45361 aagcgccccc gcgaagccct gctcgacagc ctgaaaaaac tccagcttga ttatatcgac
    45421 ctctacttaa tgcactggcc cgttcccgct atcgaccatt atgtcgaagc atggaaaggc
    45481 atgatcgaat tgcaaaaaga gggattaatc aaaagcatcg gcgtgtgcaa cttccagatc
    45541 catcacctgc aacgcctgat tgatgaaact ggcgtgacgc ctgtgataaa ccagatcgaa
    45601 cttcatccgc tgatgcaaca acgccagcta cacgcttgga acgcgacaca caaaatccag
    45661 accgaatcct ggagcccatt agcgcaagga gggaaaggcg ttttcgatca gaaagtcatt
    45721 cgcgatctgg cagataaata cggcaaaacc ccggcgcaga ttgttatccg ctggcatctg
    45781 gatagcggcc tggtggtgat cccgaaatcg gtcacacctt cacgtattgc cgaaaacttt
    45841 gatgtctggg atttccgtct cgacaaagac gaactcggca aaattgcaaa actcgatcag
    45901 ggcaagcgtc tcggtcccga tcctgaccag ttcggcggct aacgtgcaaa ttctcccggt
    45961 ggcggtaatg ttccgctacc ggacttttca gaaatcattt attcccctcg cgtcccgccc
    46021 gttgttactc ttccttgttc aggaatgcca aatataagga catcatcatg cagagccgga
    46081 agctcttaaa agaacaactc atctatatcc gggataaacg caacggagag gtgaaaaaca
    46141 gatgaaaata atacttctgt ttttagcagc cctggcaagt tttaccgtac acgcgcagcc
    46201 tccttcacag accgtagaac aaacagtccg gcagatttat cagaactata aatcagatgc
    46261 cagtacgcct tatttcggtg aaaccggaga gcgggcgata acttcagcgc gtattcaaca
    46321 ggcgcttacc ctgaacgaca atcttacact accgggcaat attggctggc tggattatga
    46381 tccggtttgt gattgtcagg attttggcga tctggtgtta gaaagcgtag cgataaccca
    46441 aactgacgtc gatcatgccg atgccgttgt gcgctttcgt atctttaaag atgataaaga
    46501 aaagaccaca cagacactga aaatggtggc ggaaaatggt cgttgggtca ttgatgatat
    46561 tgtcagcaat catggcagcg tcttacaagc agttaatagc gagaatgaaa aaacgctggc
    46621 cgctttagct tcgttgcaga aagaacagcc agaatccttt gttgctgaac tctttgaaca
    46681 tattgctgat tatagctggc cgtggacgtg ggtggtttcc gactcttacc gccaggcggt
    46741 taatgccttc tataaaacca ccttcaagac ggccaataat cccgatgaag atatgcaaat
    46801 agaacggcaa tttatttacg acaatccgat ctgttttggc gaagagtcgc tattttcacg
    46861 cgttgatgaa attcgagtcc tggagaaaac cgccgattcc gcccgcattc atgttcgttt
    46921 tacgctgacc aatggcaaca atgaagagca agagctggtt ttacagcggc gcgaaggtaa
    46981 gtgggaaatc gctgatttta tccgcccgaa cagcggcagc ctacttaagc agattgaggc
    47041 aaaaaccgcc gccagattaa agcaatgagc tgaattaaat aacaattagc cggaacaata
    47101 aataaaaggg aacactatat gaaaacgatt ttcaccgtgg gagctgttgt tctggcaacc
    47161 tgcttgctca gtggctgcgt caatgagcaa aaggtcaatc aactggcgag caatgtacaa
    47221 acattaaatg ccaaaatcgc ccggcttgag caggatatga aagcactacg cccacaaatc
    47281 tatgctgcca aatccgaagc taacagagcc aatacgcgtc ttgatgcaca ggactatttt
    47341 gattgcctgc gctgcttgcg tatgtacgca gaatgataaa aaaatccccg gcagcatgtc
    47401 agttgccggg gatttttttt aacgtccaac cgccgcttta gggcgtttct tcgcaccagc
    47461 attcaccgga cgagattgcg tagacgacgc tttttttgcc gtagcaggcg tctgacgctg
    47521 agtcgccatc ggcgtatgtt tagtcaacgc cggacgggta ttgcggttct ggcgacgagc
    47581 ttcacgcatc tcttcaatgg ttggcgcagg cactaagcaa tcgcgacggc tgccaatcag
    47641 atgctttttg cccatcgctt ccagcgcctg gcggattaac ggccagtttg ccggatcgtg
    47701 gtaacgcaac aacgctttat gcaaacgacg ctgtttgtcg cccttcggta cgaagacgcc
    47761 ttcgctctta taaccaatct tcgccagcgg ttttttcccg gtgtaataca tggtggttga
    47821 gttcgccagc ggcgatgggt agaagttctg tacctggtcg agacggaaac gatgcttttt
    47881 cagccacagc gccaaattca ccatatcttc atcacgcgta ccggggtgcg cagagatgaa
    47941 atacgggatc agatactgct ctttacctgc ctgtttcgag taggtatcga acagctcttt
    48001 aaagcggtca tagctgccca tgcccggctt catcatcttc gacaacggcc cttcttcggt
    48061 atgttccgga gcaatcttca gataaccgcc gacgtgatgg gtcgccaact ctttgatata
    48121 gcgcggatct tccacggcga tgtcataacg tacgcctgag gcgataagga tttttttaat
    48181 gcctttcaga tcacgcgcac ggcgatagag gttgatcgtc ggttcgtggt tagtgtccat
    48241 gtgcggacaa atatccggat aaacgcacga caaacggcga caagtttgtt cagcgcgtgg
    48301 cgatttgcag cgcaacatat acatgttggc agttggccca ccgagatcgg aaatcacgcc
    48361 cgtaaaacct ggaacggtgt cgcggatcgc ttcgatctca ttaatgatcg aatcttccga
    48421 acggctctga ataatgcgcc cttcgtgctc ggtaatagaa cagaaagaac agccgccaaa
    48481 gcagccgcgc ataatgttga ccgaaaaacg gatcatttcg taagccggaa tacgggcatt
    48541 gccataggcc ggatgtggca cgcgcttgta cggcagcgca aaaacgctgt ccatctcttc
    48601 ggtagaaagc gggatagcag gcgggttgat ccacacatag cggtcaccgt gtttttgcat
    48661 caatgcgcgg gcgcagcctg ggttggtttc gtggtgcaga atgcgcgaag catgggcgta
    48721 cagcacttta tcgcccttca ctttctcgaa agaaggcagc aatacgtagg ttttttccca
    48781 cggtttctgg cgcggtggct gcacgattac agctttcgct tcctgctttt tcggcgcaac
    48841 aggtttgtta tccgcacacg gcaaatcttc gccatacgga tgcgggattg ggtcgatttt
    48901 ccctagggta tcaagacggg tggaatccac accgctccag ccaggcagcg cctcttttac
    48961 gataattgcg gtattacgca catcgcggat ttcactaatc ggctcgccca tcgccagacg
    49021 atgcgctact tccaccagcg gacgttcacc gttaccaaac atcagcatgt cggctttcga
    49081 atctaccaga acggaacggc gcacggtatc ggaccagtaa tcataatgcg cggtacggcg
    49141 caggctagcc tcaataccgc cgaggatcac cggcacatct ttccacgcct ctttacaacg
    49201 ctgggtataa accagcgtgg cgcgatccgg tcgcttaccc gcgacattat ctggcgtgta
    49261 ggcatcatca tgacgtaaac ggcgatcggc ggtatagcgg ttgatcatcg aatccatgtt
    49321 gccagcagta acaccaaaaa acagattcgg tttacccaga cgcataaagt cgtctttgct
    49381 gctccagtcc ggctgggcga tgatcccgac gcgaaagccc tgcgcttcca gcatacgacc
    49441 gcaaatcgcc atcccgaagc ttgggtgatc gacatacgcg tcgccagtaa ccaaaatgat
    49501 gtcgcagcta tcccagccaa gttgatccat ctcttcacga gacatcggca aaaacggtgc
    49561 cggtccaaaa caggcggccc agtactgcgg ccaggagaac aggtcgcgat ccggttggat
    49621 cagggagata gagctcattt tgcttccaga aatgataaaa aaataatcaa aggccgggga
    49681 ttataagccg gaacgaaaga gaaatcgaaa ggtattccat actcgccctc ctcgggcgag
    49741 tatgaagatt acggtaccgg attgaccaac agttgcccaa tcgagccacg gtccgccatt
    49801 tccagcgtct gactgttgaa gtagaacggg aagtgcgccc aggaaggctg accgaaataa
    49861 acaagcagct ccacttgtcc gtcaacccaa acggtatctt tccagcctct gtcttccggg
    49921 aacggcatcg cgccattcac gttacggatc tggaacatta cgccttcaat atggaacgct
    49981 tgcggctcgt ccgcgcgtac cgtccagcgt tcccacgttc cttgctgcgc ggtgacatca
    50041 atacggttga cgtcccacag ctgtccatta atacccgggt catcacccag actgatatcg
    50101 cgactgcgaa ttggcgaacc agccatgatt tcagttggca gcaagcgcat cggaagactg
    50161 tctgtgacca gcggcagaag gccggttggg cgcagcgtta gcaccaaggt agaaaccaga
    50221 atactggatg gctcaaagaa gccgcgaata cgatcaacaa tgctcgccgc ttcaccacag
    50281 gtgatcgaca cttcatcgcc gttgctcata tccaccagaa tctcgcggcg ctcgcccggt
    50341 gccagcgaaa gttgcttcac cgatacagga gcaggcagga atccctgatc gccagaaatc
    50401 acatgtaacg ggcgaccatc gctcatctgt agttgatagc gacgagagtt cgacgcgttc
    50461 agcagtcgca agcgcaccca gccacgcgag acttcaacgt acgggctttg tacaccgtta
    50521 accagcagcg tatcaccaac aaagccgccg cttcccggtt cgttgtattc tggcgtacca
    50581 aagttatcca gccgtttatc ctggataatg accggaaaat catccacacc ataatggttg
    50641 gggataggca gcgacttgct gacttcatct tccaccagcc acattccggc aaggccgtta
    50701 tagacctgct gagccgtgcg gttgggagta ttggcgtgat accacagagt agctgcgttc
    50761 tggcgaatag gcagtacggg tgcccagtca gcgtttggcg acatcatccg tgccggaccg
    50821 cccatcagcg ggcctggtac ctgtagcccg gccaccgtca ttgagacatt ttctgtcagg
    50881 cggttgctgt aaataagctt aacatcgtcg cccttccaga cgcggatagt cggccccagg
    50941 taacgaccat tgattcccca gaccgacgcg cgtgtccctg gcgtaaatga ccagtgcgca
    51001 cgttgtacag tcataaacag cggttgccca cggcgagatt caagtagcgg cggaacgggt
    51061 agcggttgct gttgcccggc tgcgctggcc ttcaggggaa cagcgcctgc acaaagtgca
    51121 atccccgatg cctgaatgaa ctgacgccga ctgagtgaca tatttgctcc atgtaaaact
    51181 ggctaaagta ataccgtgca ggaaagtgtt ccccgcaaaa atacagattg ccgacttaaa
    51241 cttttccggc ggcttcgcgt tctgcgactt ctttatcgag ctcggcgatt ttttgttcca
    51301 ttatcgaacg acaatgggca gccagctcac gaacctgatc tttgccatac tgactgacgt
    51361 caattggcgg cagcatttcg acaatcacca gaccgttgtg cagtcgatta agattaatct
    51421 tattcgaagt tgtagagacg cacacgggaa taatcgggac gcccgccgca attgccgcgt
    51481 gaaatgctcc agtcttgaac ggtagcaggc cgcgaccacg gctgcgggtt ccttccggga
    51541 acatccagat ggaaatacgg cgttttttga aatgattcac gacttccgca atggtgccgt
    51601 gagctttagt gcgattgttt ctgtcgatca ataagttgcc ggttaaccag tacaactgcc
    51661 cgaagaaggg gatccacagc aagctctttt tacctaccgt caccgtcggc ggttgcacaa
    51721 tgttcgatgc tgtcaccatg tcatagttgt tctggtggtt agcgatatag atagcattgc
    51781 cgtagctttc agcatctgca ggtttgcggc actcaacttt caggccgaac agtggcgcaa
    51841 gacggccaaa catatgccca aaggtggcca catgtttcgg gttacgcggg ctgaaaaggc
    51901 agtaaatgga gccgaataca cagactaaga tgctgtaaat cacggtaata ataagacgaa
    51961 agatatatag cataacaccc tctgaaggtc tgagagactg gcattgtacg tcacctgaaa
    52021 tcaggttagg gcattgttaa aagccgctct ctggaaaaga gtattgttaa tcgcaacgca
    52081 tgaataaaca acggtttcaa gggaattttt atcgtttccg ggggttgaat acgctgccgg
    52141 atgacgactt aacgtttcat ccggcgttcc ttgctagcgg gaggaaacag cgccctcccc
    52201 ggcatattac tcttcgctat caccgctgct ggcacggcga ggagagtcga tctcaacacg
    52261 atcgatacgc tgcaaaccgc gcatcaacgt accgcggcgt ccacgttcgc cagtgacttt
    52321 ctgtagctct tccggacgca gtttaatttt gcgtttccca acatgaatgg tcagcgtgct
    52381 ttgcggcggc agaacgtaca gttgcgccag accatcctcg ccacgcgcgg cttctgccga
    52441 tggaatgttg ataatcttgt tgcctttgcc cttcgacagc tgcggcagat cgcttaccgg
    52501 gaacatcaac atacggcctg cctgagtgat tgccagcagc atatcggaag catcttcaat
    52561 caccaccggc ggcataacat gggcattttc cggtaaggtg atcaaagcct tacccgcacg
    52621 gttacgcgct accagatcgt taaaggtgca gacgaaaccg taacccgcat cggaagccat
    52681 cagcagtttc tgatcgtcgc tttccatcag catatggtca acggtcgccc caggcggcaa
    52741 cgttaatttg ccggtgagtg gttcgccctg accacgcgcc gacggcagcg taatcgggtc
    52801 gatggcatag ctacgaccgg tggaatcaac aaacactacc ggttggttac tcttaccttt
    52861 caccgccgct ttgaagctat cacccgcttt ataattcagg cccggcgcgt cgatatcatg
    52921 gcctttagcg ctgcgtaccc agcccatctg cgacagcaca atggtgacag gttcagacgg
    52981 cagcatgtcg tgctcgctca tcgctttcgc ttcttcgcgt tcctgcaacg gcgaacgacg
    53041 atcgtcaccg taggcttgcg cgtctgcctg cagttctttc ttcagcaggt tattcatttt
    53101 acgctcggaa gccaaaatgc cctgcaactg gtcgcgctct ttttccagct cactctgctc
    53161 accgcgaatc ttcatctctt ccagtttggc aagatgacgc agtttcagtt cgaggatcgc
    53221 ttccgcctgg gtttccgtaa ggccaaaccg cgacatcagc gccggtttcg gttcatcttc
    53281 gttacgaatg atctcaatca cttcgtcgat gttgagaaac gccaccagca aaccttccag
    53341 gatatgcagg cgcttgagga ctttctccag acgatagttc agtcggcggc gcacggtatc
    53401 gcgacggaac accagccatt cggagaggat ttccagcagg tttttcaccg ccggacgacc
    53461 atccagaccg atcatattga ggttaatgcg atagctcttt tccagatcgg tggtagcgaa
    53521 gaggtggttc atcacctgat ccatatccac gcggttggaa cgcggcacaa tcaccagacg
    53581 ggtcgggttc tcgtggtcag attcatcgcg cagatcgtca accatcggca gctttttgtt
    53641 gcgcatttgc gcagcaattt gctccagtac gcgcgcacct gaaacctgat gcggtaatgc
    53701 gctgataacc accgcgccat cttctttctt ccacaccgcg cgcatacgca ctgaaccacg
    53761 tccgttctcg tagattttac gaatctcggc gcgcgaagtg ataatttccg cttcagtcgg
    53821 ataatccggc ccctgcacga tatccagcag ctgatcgagc gtagttttcg gctggtcgat
    53881 taatgcgatt gccgcctgag ctacttcacg caggttatgc ggtggaatat cggtcgccat
    53941 gccgacggca ataccggtgg tgccgttaag caaaatgttt ggcagacggg caggtagcat
    54001 tttcggctcc tgcaaagtgc cgtcgaagtt tggcacccag tcagccgtcc cctgccccag
    54061 ctcgctcaat agcagctcgg aatatttcga caaccgggat tcggtgtaac gcattgccgc
    54121 gaacgatttc ggatcgtccg gcgcgcccca gttcccctga ccatcaacca gcggataacg
    54181 gtaagagaac ggctgcgcca tcaggaccat cgcttcataa caggcgctat cgccgtgcgg
    54241 atggtattta cccagtacgt caccgacggt acgggccgat tttttaaatt tggcgctggc
    54301 attcaggccc agttcagaca tcgcatacac aatgcggcgc tgaacaggtt tcagaccatc
    54361 accaataaac ggcaacgcac ggtccatgat cacgtacatg gagtagttta agtaggcgtt
    54421 ttccgtaaat tcatgtagcg caaggcgctc tgccatatcg ctcattaatt ctgattcctc
    54481 aacttattcg ccagccttca aaggggcaat attgccggag atactacatt atctgccgcg
    54541 tcgagtcaca ggagatcagc gtgaatagcg ccgccggacg tgctccgacg gcgataaagg
    54601 catcaatgtg ccttgatata caactctttg ctgtagactt gatcggcata gagggcagca
    54661 caaggttgcg cccatgccac accactcggc atgggggtcc gctccgggcg attcgagaag
    54721 ttgaggttat gcgagtccag ggaagcctaa agcatcaaag tagtcgactg taagtatacg
    54781 gttcagccca gtttagctgt gtctccacca gtgccccgcg cctcctgcca taagtgcggc
    54841 ttgctcatgg atatccccga gacggcttac ggcgcaccgg ataataaatt tcgcgtatac
    54901 atggtattcc ttaccggaga aattataagc ccaacaacaa tacgcctgtg acattgttcg
    54961 ctcaaactta tcgcgagtga tttggctcac actccggtaa agattcccca tcacgccaac
    55021 ggcacataaa catccgtttc cagtcgctgc tctgtcaccc cttcggcaag attggtgtag
    55081 tgaaacagaa tcggtgcttt acgcattttc tcgccgcttg caggcagcca gtggcgaata
    55141 atgccccata tcgtgtgtga aatatcgtcc agctcgccaa cgtggcgggc cacggcataa
    55201 cgtccaccgg taagttcacc attgctcaca ccataacgat tatcgggaat cggttcgcta
    55261 acgctgccgc agatatcaaa gcgaaacgct tccggtgcgg tggtggcagg ggcgtcccag
    55321 gcgacgccaa aagtctggct ttggttaaca ggagaaagcc ccgtctcttt acgccacatg
    55381 ataaatttcg ccacactgta gttgagcaac tcagggctac agcggtgcgg caacatcgcc
    55441 acagggattg atgggaaatc gataatgttt acatccagtg tcaggtttgt cattttgcct
    55501 ccctgcgaaa ttattcccgg gaacgcgtcc cgggagcggt aacaattacg gatttacttt
    55561 gcggatctgt ttgacgtcaa tttcaacaga attccagtct ttatcgactt caccctgaat
    55621 ctcaaccgta tctttcggcg tcaccgtcac accgttccag cgtttgtgat cgatatcaac
    55681 attgatagta ccgctggcat ctttaaacac gtagagatcg tcagagatgc gttcaacgat
    55741 attgccgcgc agggttaccc aggtgtcgtc acgcagggat tttgcgcttt ctacagtcgt
    55801 tacgctgccg ttcggcccct gaaatcctcc ggcctgactt tgcgttaccg atgggccaga
    55861 aaaaccgccc tgctctgctg ccatcaccgg tgcactgcac agggccatta ctgcgattac
    55921 tgctgcgaat tttttcatgt ttattactcc ctttaatgtc tgtttccgag catttaacaa
    55981 gatagtcctt aacaacttct taagggaaaa aaataaaatt taatgctgta cagagcgcgt
    56041 tacaacacgg tttactggca gcaaatacgg ttatcgcagg gatgaaaaaa tgcgaatttt
    56101 actgatagaa gatgacatgc tgattggtga cggcatcaaa actggcctta gtaaaatggg
    56161 ttttagtgtc gactggttta cacaaggtcg tcagggaaaa gaggcgcttt atagcgcacc
    56221 ttatgatgcg gtgatcctgg atttaacctt accaggcatg gatggtcgcg atattttgcg
    56281 cgaatggcga gagaaaggtc agcgtgagcc ggtactgaac ctgaccgcgc gtgatgcgtt
    56341 agcggaacgt gtagaagggc tgcgtctggg agctgacgat tatctgtgta aaccttttgc
    56401 gttgatagaa gtcgccgcca ggctggaggc tctgatgcgc cgaaccaacg gccaggccag
    56461 caacgagctg cgccacggta acgtcatgct cgaccccggc aaacgtatcg ccacgctggc
    56521 tggcgaaccc ttaacactga aaccaaaaga atttgccctg ctggaattac tgatgcgtaa
    56581 cgctggtcgg gtactgccgc gcaaactgat tgaagagaaa ctgtatacct gggacgaaga
    56641 ggtcaccagt aatgccgttg aagtgcatgt gcatcatctg cgacgcaaac tcggtagtga
    56701 ttttattcgt accgtgcatg gtattggcta cacattaggt gagaaatgaa atttacccaa
    56761 cgtcttagtc tgcgcgtcag gctgacgcta atctttttaa ttctggcctc ggtgacctgg
    56821 ctgctttcca gctttgtcgc ctggaaacaa acaacggata acgtcgatga attgttcgac
    56881 acccaactga tgctgtttgc caaacggtta agtacgctcg atctcaacga aatcaacgcg
    56941 gcggatcgca tggcacagac gccaaataaa ttaaaacacg gtcatgttga tgacgatgcg
    57001 ctgacctttg ccatctttac ccacgacggc agaatggtcc ttaatgatgg cgataacgga
    57061 gaagatattc cctatagcta tcaacgggaa ggttttgctg acgggcaact ggtcggtgaa
    57121 gacgatcctt ggcgttttgt ctggatgacc tcacctgatg gcaaatatcg catcgttgtt
    57181 ggccaggaat gggaataccg tgaagacatg gcgctggcga ttgttgccgg gcaattgatc
    57241 ccgtggctgg tcgcactgcc gattatgtta atcatcatga tggtactact gggtcgtgaa
    57301 ctcgcgccgc tgaacaaact ggcgctggca ctacgtatgc gtgaccctga ctcggaaaaa
    57361 ccactaaacg cgactggcgt acccagcgaa gtgcggccac tggttgagtc gctaaatcaa
    57421 ctgttcgccc gcacacatgc gatgatggtt cgagaacgac gctttacctc cgatgcagct
    57481 cacgaactac gtagcccgtt aacagcgctg aaagtgcaaa ccgaagttgc gcagctctct
    57541 gacgatgatc cgcaggcgcg gaaaaaagca ctgctccaat tacattccgg gatcgatcgc
    57601 gctactcgtc tggttgatca actgctcacg ctatcgcggc tggactcact ggataacctt
    57661 caggacgtcg cggagatccc gcttgaagat ctcctacaat cgtcggtgat ggatatttac
    57721 cacacggcgc agcaggcgaa aattgacgtg cgactgacac tcaatgccca cggcatcaaa
    57781 cgcaccggac aaccgctatt gctaagtttg ttggtgcgaa atttgctgga taacggcccc
    57841 ggtgtgacac cagaggcact ggcgcgaatt ggcgaacgct tctatcgccc acccggacaa
    57901 accgctaccg gcagtggact tgggctatcg attgtccagc gaatcgccaa gctgcatgac
    57961 atgaatgttg agtttgggaa tgcggaacaa ggtggatttg aggcgaaggt aagctggtaa
    58021 cttctctgtc tatgagagcc gttaaaacga ctctcataga ttttactaat agcaaaatat
    58081 aaaccgtccc caaaaaagcc accaaccaca aaccagacaa ataacgagcg agaatacata
    58141 tagccaaaac tcgaacattt cccgcataaa gagttttctt aagataagaa taataagtgg
    58201 cgtaagaaga aaaaatgctg caatcattgc ttgcaatata cgatcttcgc ctgaaacgga
    58261 aggtatactg caaatattgc ctcccgactc aagcatccag tcatacttat tgaaagcaaa
    58321 aaaagaatct attaaccaat aagtaagcat tatatatagc aatgcttcaa atattgtttt
    58381 aattttctgc tttaacatat atcagtacgc tcataaaaat ccatttataa ttatcaacat
    58441 gacacgttga tattttcatc ttttacttat tcaaagccat attttcaatc cccaaaaaaa
    58501 ccgaaccaaa cgtgtaaatt attccggtgc gagtaagaat tatgaggaat ggctatcagt
    58561 attgtcattt tcagaaaata tttatcctgc atcggtgagt cagagctaga tcagactttt
    58621 gctaaattcg caaaagactt tgcacatttt gctaatttca cagtaccgct ctgtgacgta
    58681 ctatagtcga caaacgtctc accttgagat taaaaaatga gcaacatcct gattatcaac
    58741 ggcgcgaaaa aattcgccca ctccaatggt caactgaacg acaccctgac cgaagtcgcg
    58801 gatggcacac tgcgcgacct cgggcatgat gtccgcatcg ttcgcgccga cagcgactac
    58861 aatgtcaaag cggaagtaca aaactttctc tgggctgatg tggtgatctg gcagatgcca
    58921 ggctggtgga tgggcgcgcc gtggacagtg aaaaaatata ttgatgatgt attcaccgaa
    58981 ggtcacggga ctctgtatgc cagcgatggt cgtacccgca aagatccgtc gaaaaaatac
    59041 ggttccggca gcctggtaca ggacaaaaaa tatatgcttt ctctaacctg gaacgcacca
    59101 atggaagcct tcaccgaaaa agatcagttc ttccacggcg ttggcgttga cggtgtgtat
    59161 ctgccgttcc ataaagcaaa ccaattcctc ggtatggaac cgctgccgac atttatcgct
    59221 aatgacgtga taaaaatgcc tgatgttccc cgctatactg aagaatatcg caagcatctt
    59281 gtggaaattt ttggttaact agagctcagg ctttagaagg agttaaccat gcttaccgta
    59341 atcgcagaaa tccgtactcg tcctggtcaa catcaccgtc aggcggtatt ggatcagttt
    59401 gctaaaatcg ttccaaccgt actgaaagaa gaaggttgcc acggctatgc gccaatggtg
    59461 gattgcgctg ctggcgtgag tttccagtct atggcaccgg attctatcgt gatgattgag
    59521 cagtgggaaa gcatcgcgca ccttgaagcg catctgcaaa ccccgcacat gaaggcgtac
    59581 agcgaagccg taaaaggcga cgtgctggag atgaatatcc gtattctgca gccagggatt
    59641 taatcctgcc ttgtttgccc ggccatcctg accgggcaat gttctttcct ttaaacctca
    59701 atctccgcca tgtcgccttt ctcttgcaac cagttgcggc gatcttccga gcgtttcttc
    59761 gccagcagca tatccatcat cgcgtcagta cgctgatcgt cttcatcatc gatagtcaac
    59821 tgcaccagac ggcgagtgtt cggatcaagc gtggtttcac gcaattgcat cgggttcatt
    59881 tcccccagac ctttaaaacg ctggacgttc ggcttgcctt tcttgcgttt taattgctca
    59941 agtacgccct ctttctcttc ttccgtcagc gcgtaataaa cctctttccc gagatcaata
    60001 cggtagagcg gtggcagtgc gacgtaaacg tgaccgtgtt tcaccaacgc gcggaagtgt
    60061 tttacgaaca aagcgcagag cagcgtggca atgtgcagac catcagagtc cgcatccgcg
    60121 aggatacaga ttttgccata acgaagctgg ctcagatcgt cgctgtcagg atcgataccg
    60181 atcgctaccg aaatatcatg cacttcctgc gaggccagca cttcgtcgga agaaacttcc
    60241 caggtgttaa ggatcttacc tttcagcggc atgatcgcct gatattcacg atcgcgcgcc
    60301 tgcttggcag atccgcctgc ggagtcacct tccacgagga acagttcggt acggttaagg
    60361 ccctgcgcgg tacaatcagc cagtttgcca ggcagcgccg ggccgctggt cagcttttta
    60421 cgcaccactt ttttagccgc acgcatacga cgctgggcgc tggaaatcgc catctctgcc
    60481 agcaactccg ccgcctgaac gttctggttc agccacagga tgaaggcatc tttcaccacg
    60541 cccgaaacga atgccgcaca ctgacgcgaa gagagacgct ctttggtctg cccggcaaac
    60601 tgcggatcct gcattttaac cgacagaaca taggcgcagc gatcccagat atcttccgcc
    60661 gacagcttca cgccgcgcgg cagaatgttg cggtattcac agaactcacg catcgcgtcc
    60721 aacaggccct gacgcagacc attaacatgg gtaccgccct gcatcgttgg gataaggttg
    60781 acgtagcttt cggtcagcag ttcaccgcct tccggcagcc acagtagcgc ccagtccaca
    60841 gcttcagtat cgccagcgaa attaccgata aacggttttt ctggcagcgt cggtaaaccg
    60901 tttaccgctt ccgccaggta atcattcaga ccgtcctgat agcaccagcg ctgttcggtg
    60961 ttgttgatct catctttaaa ggtaatctca acgccggggc acagtactgc tttggccttc
    61021 agcacatgcg tcaggcgtga aacagaaaaa cgcgggctgt caaagaaggt ttcatccggc
    61081 cagaagtgca cgctggtacc ggtattgcgt ttaccgcaag tgccgacaac ctgtaaatcc
    61141 tgcacctttt cgccattttc aaaggcgatg ttataaacct gaccatcgcg gcgcacgtta
    61201 acttctacgc gcttcgacaa ggcgttaact accgaaatcc ccacgccatg caggccgcca
    61261 gagaactggt aatttttgtt agagaattta ccgcctgcat gcagacggca aagaatcagt
    61321 tcaaccgccg gtactccttc ttccgggtga atatccaccg gcatcccgcg cccatcgtca
    61381 atcacttcta acgactggtc agcatgtaag attacatcca cgcgttttgc gtgacccgcc
    61441 agtgcttcat cgacactgtt atcaataact tcttgcccca aatggttagg gcgagtggta
    61501 tcggtataca tccccggacg gcggcgaacc ggctcaagcc cggtgagtac ctcaatggca
    61561 tcagcgttat aagtttgcgt catggtttaa gttagcaatt cgagttgatc gtcagagatg
    61621 gtgcagacca agaaaatcga cgatcgggtt gaaataatct tcgaagcccg tgaatgcgtg
    61681 gttgccgcct tctatgacag tctggcggca ggaagcatag tacgccaccg cctggcggta
    61741 atccagcact tcatctcccg tctgttgcag cagccagatc aaatccggcg cttccagcgg
    61801 gtcaatctgc atgactttga gatcgtaaat atggcgtgac tctagcacat attgctgccc
    61861 ggtgtagggg ttctcgttct gaccgagata gtccgtcagc aattcaaacg ggcgtaccgc
    61921 cgggtttacc accactgcgg gcagcataaa acattgcgac aaccaggtgg cgtaatatcc
    61981 ccccagtgac gaaccgacaa tacccagcga atcgccgcca tgttccagga caatggattc
    62041 cagcagttct gccgcgtcgg aaggatacgg cggcaactgc ggaatgatca tctcaacgtc
    62101 agggtgatgt tccgccagcc agttttttaa caagctcgct tttgcagagc gcggcgagct
    62161 gttgaaaccg tgtaaataaa gaagcgtaga catcagtagc cttctgaagc ggtatcaggt
    62221 tggaaacgtg tgtccgccag gcgatgcacc tcggtggtca gcgtgccatc agcatgtaac
    62281 tcgagagtac gccagccggg cgcgatagta tccagcgtaa agttggaaca gtgcggctta
    62341 aactgcacac aggtcgacgg cgttgccagc aggcggcgac cgttccagtc gagatccagc
    62401 tcctgatgaa tatgaccgca cagcaagtat ttgacgtgcg gaaacttcgc cagcacggta
    62461 tccagttcgc ccgcgttacg cagactgtgt tgatcgagcc aactacaacc cgcaggtagc
    62521 ggatgatgat gcagcagcag caacgtatgg cgttctggcg catcggccag tttacgttcc
    62581 agccactcaa gctgaaactc gctcagctca ccgtgcggca cgccaaacac ctggctatcc
    62641 agcaacagga tttgccattg ctcaccaata aacacccgct tcgccgggga gatacccgca
    62701 tcctgtaacg cgctgtacat cgcgggctgg aaatcgtggt tgcccggcag ccagacgcag
    62761 ggcgcacgaa aacttgcgat gccttcagcg aaatgctgat aggccgcaga ggattgatcc
    62821 tgcgctaaat cacctgtcgc gacaatcagg tcgaattcgt gctggtgtgg acgaatcgcc
    62881 tccagcaccg cctggtagct ttcccaggtg tttaccccta acagggcttc gtgcttttgt
    62941 gcaaacaggt gagtatcggt aatttgtaaa atccttcctc tggcctcacc agccagagga
    63001 agggttaaca ggctttccaa atggtgtcct taggtttcac gacgctagta aaccggaatc
    63061 gccatcgctc catgtgctaa acagtatcgc aaccagtccg ctaaaaactg gttaatttga
    63121 tgcttttcgt cgcgttgatg caacttttta ttaggataat cataccgcgc tttgaagcga
    63181 aaaatctgct ggcttgaaca cacttcagcc accatcgcgt catgatacag acgcaccgtc
    63241 attgacggaa ggctccagta actgatcgcg ggcgcagtct gttctattgt caccagggta
    63301 gtgtatcggg tcgattccac aatcgtcagc cgatattgtg cgtttgccac ctgatagctt
    63361 acagtttcgc cgggtgcgtc attgcgcggt aacaaacggc gcaattgtga aaagttcatc
    63421 tcgcacaggc gcatcatttc aggaaagtca ggtgtgtaac gcttcattta tgcccactca
    63481 ttttttaacg cttgatgatg cagctgcagc cattgcaaag cgatgaccga cgctgcgttg
    63541 tcgattttcc cctcttctac ccactggtat gcctgttccc ggcttaccac atgaacgcga
    63601 atatcttcgt tttcatcagc cagaccatga ataccgcttg cggtcgtggc gtccacttcg
    63661 cccaccataa ttgacgaacg ctcactggtg ccccccgggc ttgccaggaa acttaacacc
    63721 ggtttggtcc gtttgactat cagtcccgcc tcttcaatcg cttcgcgacg ggcaacatct
    63781 tccacacttt caccctcttc aatcatcccg gcaaccatct ccagtagcca gggggtttcg
    63841 ctggtgtcgt acgcggcaat ccgaatctgc tcaatcagca caacttcatc acgcactggg
    63901 tcaaagggta gcaagactgc ggcgtgaccg cgctcaaaaa tttcccgccg tacctcatga
    63961 ctcatttgcc cgttgaatag acgatgacga aatctataaa gatctaatga aaaaaagccg
    64021 cgataaagtg tttctcgtgc aataatttct acatcgtttt tgccaaatgt aacgggcagg
    64081 ttgtctggct taagcattgt taatgtccta gcactaatag tgaattaaat gtgaatttca
    64141 gcgacgtttg actgccgttt gagcagtcat atgttaaatt gaggcacatt aacgccctat
    64201 ggcacgtaac gccaaccttt tgcggtagcg gcttctgcta gaatccgcaa taattttaca
    64261 gtttgatcgc gctaaatact gcttcaccac aaggaatgca aatgaagaaa ttgctcccca
    64321 ttcttatcgg cctgagcctt tctgggttca gttcgttgag ccaggccgag aacctgatgc
    64381 aagtttatca gcaagcacgc cttagtaacc cggaattgcg taagtctgcc gccgatcgtg
    64441 atgctgcctt tgaaaaaatt aatgaagcgc gcagtccatt actgccacag ctaggtttag
    64501 gtgcagatta cacctatagc aacggctacc gcgacgcgaa cggcatcaac tcgaacgcga
    64561 ccagtgcgtc cctgcagtta actcaatcca tttttgatat gtcgaaatgg cgtgcgttaa
    64621 cgctgcagga aaaagcagca gggattcagg acgtcacata tcagaccgat cagcaaacct
    64681 tgatcctcaa caccgcgacc gcttatttca acgtgttgaa tgctattgac gttctttcct
    64741 atacacaggc acaaaaagaa gcgatctacc gtcaattaga tcaaaccacc caacgtttta
    64801 acgtgggcct ggtagcgatc accgacgtgc agaacgcccg cgcgcagtac gataccgtgc
    64861 tggcgaacga agtgaccgca cgtaataacc ttgataacgc ggtagagcag ctgcgccaga
    64921 tcaccggtaa ctactatccg gaactggcgg cgctgaatgt cgaaaacttt aaaaccgaca
    64981 aaccacagcc ggttaacgcg ctgctgaaag aagccgaaaa acgcaacctg tcgctgttac
    65041 aggcacgctt gagccaggac ctggagcgcg agcaaattcg ccaggcgcag gatggtcact
    65101 taccgactct ggatttaacg gcttctaccg ggatttctga cacctcttat agcggttcga
    65161 aaacccgtgg tgccgctggt acccagtatg acgatagcaa tatgggccag aacaaagttg
    65221 gcctgagctt ctcgctgccg atttatcagg gcggaatggt taactcgcag gtgaaacagg
    65281 cacagtacaa ctttgttggt gccagtgagc aactggaaag cgcacatcgt agcgtcgtgc
    65341 aaaccgtacg ttcctccttc aacaacatta atgcttctat cagtagtatt aacgcctaca
    65401 aacaagccgt agtttccgcg caaagctcat tagacgcgat ggaagcgggc tactcggtcg
    65461 gtacgcgtac cattgttgat gtgttggatg cgaccaccac gctgtacaac gccaagcaag
    65521 agctggcgaa tgcgcgttat aactacctga ttaatcagct gaatattaag tcagccctgg
    65581 gtacgttgaa cgagcaggat ttgctggcac tgaacaatgc gctgagcaaa ccggtttcca
    65641 ctaatccgga aaacgttgcc ccgcaaacgc cggaacagaa tgctattgct gatggttatg
    65701 cgcctgatag cccggcaccc gtcgttcagc aaacatccgc acgcactacc accagtaacg
    65761 gtcataaccc tttccgtaac tgatgacgac gacggggctt cggccccgtc tgaacgtaag
    65821 gcaacgtaaa gatacgggtt atctgccgca ttcttccccc ttctcgcttc aatttcgacc
    65881 agccatcctc tattctgatg ggtatttacc actggtcccg gaagacaaaa atgaaacgga
    65941 caaaatccat acgccacgca tcgttccgca aaaactggag cgcacgccat ctgacaccag
    66001 tcgctctcgc ggttgccact gtttttatgc tggctggctg tgaaaagagt gatgaaacag
    66061 tgtctctcta tcaaaatgct gacgactgtt cagctgcaaa cccaggcaaa agcgccgaat
    66121 gtaccaccgc gtacaacaat gcgctgaaag aagccgaacg tactgcgccg aaatacgcca
    66181 cccgtgaaga ctgtgttgct gaatttggtg aaggtcagtg tcagcaagca ccagcccagg
    66241 ctggcatggc accagaaaac caggcgcagg ctcagcaatc cagcgggagt ttctggatgc
    66301 cgctgatggc cggttacatg atggggcgtc tgatgggcgg cggcgcggga tttgcacagc
    66361 agccgctgtt ctcctcgaaa aacccggcca gtccggctta cggtaaatat accgacgcga
    66421 cgggtaaaaa ctatggcgca gcccagccag gccgcaccat gaccgtaccg aagacggcaa
    66481 tggcaccaaa accggcgacc accactaccg ttacccgtgg cggttttggt gaatctgttg
    66541 ccaaacaaag cactatgcag cgtagcgcaa ccggcacctc ttctcgttca atgggtggct
    66601 gataccgatg gaaagagtca gtattaccga gcgcccggac tggcgtgaga aagcccacga
    66661 atacggtttc aattttcaca ccatgtacgg cgagccgtac tggtgtgaag atgcttacta
    66721 caagttgacc ctcgcccagg ttgaaaagct ggaagaagtc accgccgaac tgcaccagat
    66781 gtgcctgaaa gtggtggaaa aagtgatcgc cagcgatgag ctgatgacca aattccgcat
    66841 accaaaacac acctggagtt ttgtgcgtca atcatggctg actcaccagc catcgcttta
    66901 ttcgcgtctt gatctggcgt gggatggcac tggcgaacct aaacttctgg aaaataacgc
    66961 cgatacgcca acgtcactat acgaggcggc gttctttcag tggatctggc tggaagatca
    67021 gcttaacgcc ggtaacttgc cggagggcag cgaccagttt aacagtctgc aagaaaaact
    67081 gatcgatcgc ttcgttgagc tgcgtgaaca gtatggcttc cagttgctgc atctcacctg
    67141 ctgtcgcgac acggtggaag atcgcggaac cattcagtat ttgcaggact gcgcaaccga
    67201 agctgaaatc gccaccgagt tcctctacat cgacgagata ggtttaggcg aaaaaggcca
    67261 gttcacggat ttacaggatc aggtgatttc taacctgttc aagctttatc cgtgggaatt
    67321 tatgctgcgc gaaatgttct ccaccaagct ggaagatgct ggcgtacgct ggctggaacc
    67381 ggcgtggaag agcattatct ccaacaaggc acttctaccg ctactgtggg agatgttccc
    67441 gaatcacccg aacctgctgc ccgcttattt tgcggaagat gatcatccgc aaatggaaaa
    67501 atatgtggtt aaaccgatct tctcccgtga aggcgcaaac gtgtcgatca ttgagaacgg
    67561 caaaaccatt gaagcagcgg aaggtccgtg tggcgaagaa gggatgattg ttcagcaatt
    67621 ccacccgtta ccgaaattcg gcgacagcta tatgctgatt ggtagctggc tggtgaacga
    67681 tcaacctgcc ggaattggca ttcgtgaaga ccgtgcgttg atcacccagg atatgtctcg
    67741 gttttatcca catatttttg ttgaataagc cacgataccg gatggcactc gccatccggt
    67801 aattgttagc ctatctgcac cgacagcata cttaaactgc ccatttctat accatcgacc
    67861 ggaatggtaa ttggctcctg cccatcccac acacctaaca catacaacaa cggcagatag
    67921 tgctctggcg ttgggttcga taacgcgcca ccttcatggt cgagataatt caccagagga
    67981 tgctgctcta ccggcccttg ccacgtcaga ttcgctttca catactcatt aaacgacgtc
    68041 gcccacggat acggtgaact atcaccgtgc cacttcactg tgcgcaggtt atgcaccacg
    68101 ttaccgctgg cgaccaacat tattccttca tcacgcagcg ccgcgagttt gcgccccatt
    68161 tcgaaatgcc aggcggcagg tttgctactg tcgatactca actgcaccat agggatatcg
    68221 gcgtcaggat acatcttaat cagcacgccc cacgagccgt ggtcaaagcc ccaggcttct
    68281 ttatccagcg tcaccgggat cggcgctaac agctcaacca gacgctgtgc cagcgcaggc
    68341 gaacccggag caggataatg cgtatcgtac agcgcctgcg ggaagccacc aaagtcatga
    68401 atcgtgggcg gcgtctccat cgcggtcact cctgttccac gggtaaacca gtgagccgaa
    68461 accaccacaa tcgcttgcgg gcgtggcaat gtcatcccca acttctgcca gctgcgggta
    68521 tacaaattat cttccagcac gttcatcgga ctaccgtgac ctaaaaacaa tgctggcata
    68581 cgtgttgaag acatgatgat atccttaact aaaggtgtca ttttgatatc ctcacaatgc
    68641 gcttgttcgg cggagtaaga acccggataa caatgatgat gatcatcagt tattttgacg
    68701 atctgcctga aggtgaagat ttataaggag ttgtcgatgt cagtacctct cattctgacc
    68761 atactggcgg gggcagccac gtttattggc gcgtttctcg gcgttctcgg acaaaaaccc
    68821 tcgaaccgct tactggcgtt ttcgctaggt tttgcggcgg ggatcatgtt gctcatctca
    68881 ttaatggaaa tgcttcctgc cgcactagca gctgaaggaa tgtcgccggt gttgggttat
    68941 ggaatgttta tcttcggtct gcttggctac tttggcctgg accgcatgtt gccgcatgcg
    69001 catccgcagg atttaatgca aaaatcggtg cagctgttgc caaaatcgat caagcgcaca
    69061 gccattctgc tcactctcgg catcagtctg cataacttcc cggaagggat tgccaccttt
    69121 gtcacggcga gcagcaacct ggagctggga tttggcatcg cactggccgt cgcgttgcac
    69181 aatatccctg aaggtctggc agtggcaggc ccggtttatg cggcaacggg gtctaaacgc
    69241 accgcgattc tgtgggcggg gatttctgga ctagcagaaa ttcttggtgg tgtgctggcg
    69301 tggttaatcc tcggtagcat gatttccccg gtggtcatgg cggcaatcat ggcggcggtt
    69361 gcaggaatta tggtggcgct ctcggttgat gaattaatgc cgctcgccaa agagattgac
    69421 cctaataata accccagcta tggcgtacta tgtggaatgt cagtgatggg attcagttta
    69481 gtgctgctac aaacggcggg aattggttaa aaacaacatc agaagttgtc cagcacatgg
    69541 acaacttctg ccagcttcac gaaagcctta ttctaccttt tcggctgtct cttctctcgt
    69601 actgtttaag tatttgttcc gcgcttcaag cagtgcgtct ctgtcctggc gaaacgcccg
    69661 acaataacgt ttcaaatgca ggaaataacc caccacaacc agagcaatca atacaatcca
    69721 gtaccaggcg ataaacatcc ttttaccctt aatatttaac aatactaaac atttagcgca
    69781 taaatttcac atatcctttt tcggatatat tcatatggtc gcataataac ggaacaatta
    69841 tatgattttg atcataatga aaaagaatgc ctgatattaa tgaaattttc tcatagaaag
    69901 agaagcggaa ttaacatctt gcatagcagg gcaaaataaa aaccgaagcc agctacaact
    69961 tcggtttctt gattaaggca gtaattaagc agcggttttc agctggcttt acgctcatgt
    70021 gcctgacggt atgccaccag gtcttcaata gtcacgagcg ccatattgtg tttattggca
    70081 aactcaatgc actctggtgc acgcgccatc gtgccatcgt cattagtcag ctcacacagc
    70141 acaccagccg gtttaaagcc tgccagcgtc atcaaatcaa tagtcgcttc agtatgaccg
    70201 ccacgcgtca gtacaccacc tgcctgcgcg cgaagtggga aaacgtggcc cgggcgattc
    70261 agatcagaag gttttgcgcc atcggcaatc gctgcgcgaa cggtcgtaat acgatcagcg
    70321 gcagaaacac cggtagtcac accttcagcc gcttcaatgg tcacggtaaa accggtgcca
    70381 taggcgctgg tgttattttc taccatcatt ggcagatcaa gttgtttacg gcgatcttca
    70441 gtgatgcaca ggcaaacaat accgctaccg tggcgaatgg tcagcgccat ctgctcaacg
    70501 gtcatggttt ctgccgggaa gatcatatcg ccttcgtttt cacggtcttc atcatcaagc
    70561 accattacac cgcgtccttc acgcagcgca gccagtgcat tttcaacacg ttcgaaaggc
    70621 gtaccaaaag aggaaagtag cgtctgattc atggtaaaaa aacctcacta aaattatggt
    70681 taccagaatc agggcagtct taggagtggc ggcatatagc caaaataacg tgagcgggtc
    70741 catgcccgac agaatcgtta ctctctccca tccggactct aaccgtcggc cccggaatta
    70801 caccggatct gctgtccttt gagttcgcac ccaaagcgct cgcgggcttt caactgagtt
    70861 gatttaccgc cggtggggaa tttcgccccg ccctgagaat aagcggattc actataacgc
    70921 taatgattag cggcagcaac gcatagcttc acataattct ggtttatgac ttacccttat
    70981 cgcactacaa tggtactcaa cacctatcag tacagggaaa ctacaatgat tgacccgaaa
    71041 aaaattgagc aaatcgctcg ccaggttcac gaatcaatgc ctaaaggaat cagggagttc
    71101 ggggaagatg tggagaaaaa aatccgccaa accctacaag ctcagctgac gcgcctcgac
    71161 cttgtaagcc gtgaagagtt cgacgtccaa acgcaagtcc tgttacggac tcgtgagaaa
    71221 ctggcgctgc ttgaacaaag aatcagcgaa ctggaaaatc gcagtactga gatcaaaaag
    71281 cagccagatc cagagactct ccccccaacg ctgtaaacat ttgtctttaa tgcactgtaa
    71341 attatgaatt aatcctctaa tttacagtgt tttctattac attatcgctt aatcccattc
    71401 agagtatcat cacaaaaatg aatgattgca attgaaaata taattgttat gtattcttta
    71461 agttttactt aatcgata
//
LOCUS       DAJTSA010000006        70712 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000006 DAJTSA010000000
VERSION     DAJTSA010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.006"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..795
                     /locus_tag="OO369_000424"
     CDS             <1..795
                     /locus_tag="OO369_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5980818.1"
                     /translation="WLPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            806..1897
                     /locus_tag="OO369_000425"
     CDS             806..1897
                     /locus_tag="OO369_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5980819.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="OO369_000426"
     CDS             complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="OO369_000426"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR5980820.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2865..4901
                     /gene="lpoA"
                     /locus_tag="OO369_000427"
     CDS             2865..4901
                     /gene="lpoA"
                     /locus_tag="OO369_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR5980821.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4859..5254
                     /locus_tag="OO369_000428"
     CDS             4859..5254
                     /locus_tag="OO369_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR5980822.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5274..5864
                     /gene="diaA"
                     /locus_tag="OO369_000429"
     CDS             5274..5864
                     /gene="diaA"
                     /locus_tag="OO369_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR5980823.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5874..6449
                     /gene="yraP"
                     /locus_tag="OO369_000430"
     CDS             5874..6449
                     /gene="yraP"
                     /locus_tag="OO369_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR5980824.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6563..7603)
                     /locus_tag="OO369_000431"
     CDS             complement(6563..7603)
                     /locus_tag="OO369_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5980825.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7676..8311)
                     /locus_tag="OO369_000432"
     CDS             complement(7676..8311)
                     /locus_tag="OO369_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR5980826.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8439..8957
                     /gene="yhbO"
                     /locus_tag="OO369_000433"
     CDS             8439..8957
                     /gene="yhbO"
                     /locus_tag="OO369_000433"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR5980827.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8937..9380)
                     /locus_tag="OO369_000434"
     CDS             complement(8937..9380)
                     /locus_tag="OO369_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR5980828.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9431..9733
                     /gene="yhbQ"
                     /locus_tag="OO369_000435"
     CDS             9431..9733
                     /gene="yhbQ"
                     /locus_tag="OO369_000435"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR5980829.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9720..10223)
                     /locus_tag="OO369_000436"
     CDS             complement(9720..10223)
                     /locus_tag="OO369_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5980830.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10217..10741)
                     /locus_tag="OO369_000437"
     CDS             complement(10217..10741)
                     /locus_tag="OO369_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR5980831.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10950..11945
                     /locus_tag="OO369_000438"
     CDS             10950..11945
                     /locus_tag="OO369_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5980832.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11954..12832
                     /locus_tag="OO369_000439"
     CDS             11954..12832
                     /locus_tag="OO369_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5980833.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12913..13920
                     /locus_tag="OO369_000440"
     CDS             12913..13920
                     /locus_tag="OO369_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR5980834.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="OO369_000441"
     CDS             complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="OO369_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR5980835.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="OO369_000442"
     CDS             complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="OO369_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR5980836.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="OO369_000443"
     CDS             complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="OO369_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR5980837.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="OO369_000444"
     CDS             complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="OO369_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR5980838.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="OO369_000445"
     CDS             complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="OO369_000445"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR5980839.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="OO369_000446"
     CDS             complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="OO369_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR5980840.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21298..22242)
                     /gene="truB"
                     /locus_tag="OO369_000447"
     CDS             complement(21298..22242)
                     /gene="truB"
                     /locus_tag="OO369_000447"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR5980841.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="OO369_000448"
     CDS             complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="OO369_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR5980842.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22807..25455)
                     /gene="infB"
                     /locus_tag="OO369_000449"
     CDS             complement(22807..25455)
                     /gene="infB"
                     /locus_tag="OO369_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR5980843.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="OO369_000450"
     CDS             complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="OO369_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR5980844.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="OO369_000451"
     CDS             complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="OO369_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR5980845.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27654..27730)
                     /locus_tag="OO369_000452"
     tRNA            complement(27654..27730)
                     /locus_tag="OO369_000452"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27694..27696),aa:Met,seq:cat)
     gene            28078..29421
                     /gene="argG"
                     /locus_tag="OO369_000453"
     CDS             28078..29421
                     /gene="argG"
                     /locus_tag="OO369_000453"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR5980846.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29429..31036)
                     /locus_tag="OO369_000454"
                     /pseudo
     CDS             complement(29429..31036)
                     /locus_tag="OO369_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31514..31600)
                     /locus_tag="OO369_000455"
     tRNA            complement(31514..31600)
                     /locus_tag="OO369_000455"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31564..31566),aa:Leu,seq:gag)
     gene            complement(31615..31947)
                     /gene="secG"
                     /locus_tag="OO369_000456"
     CDS             complement(31615..31947)
                     /gene="secG"
                     /locus_tag="OO369_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR5980847.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="OO369_000457"
     CDS             complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="OO369_000457"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR5980848.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33505..34353)
                     /gene="folP"
                     /locus_tag="OO369_000458"
     CDS             complement(33505..34353)
                     /gene="folP"
                     /locus_tag="OO369_000458"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR5980849.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="OO369_000459"
     CDS             complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="OO369_000459"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR5980850.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="OO369_000460"
     CDS             complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="OO369_000460"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR5980851.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37232..37525
                     /gene="yhbY"
                     /locus_tag="OO369_000461"
     CDS             37232..37525
                     /gene="yhbY"
                     /locus_tag="OO369_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR5980852.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37681..38157)
                     /gene="greA"
                     /locus_tag="OO369_000462"
     CDS             complement(37681..38157)
                     /gene="greA"
                     /locus_tag="OO369_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR5980853.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38405..39838
                     /gene="dacB"
                     /locus_tag="OO369_000463"
     CDS             38405..39838
                     /gene="dacB"
                     /locus_tag="OO369_000463"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5980854.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="OO369_000464"
     CDS             complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="OO369_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR5980855.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41212..42177)
                     /locus_tag="OO369_000465"
     CDS             complement(41212..42177)
                     /locus_tag="OO369_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5980856.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="OO369_000466"
     CDS             complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="OO369_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR5980857.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="OO369_000467"
     CDS             complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="OO369_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR5980858.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43151..44122
                     /gene="ispB"
                     /locus_tag="OO369_000468"
     CDS             43151..44122
                     /gene="ispB"
                     /locus_tag="OO369_000468"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR5980859.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44349..44627
                     /gene="sfsB"
                     /locus_tag="OO369_000469"
     CDS             44349..44627
                     /gene="sfsB"
                     /locus_tag="OO369_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR5980860.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44675..45934)
                     /gene="murA"
                     /locus_tag="OO369_000470"
     CDS             complement(44675..45934)
                     /gene="murA"
                     /locus_tag="OO369_000470"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR5980861.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="OO369_000471"
     CDS             complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="OO369_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR5980862.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="OO369_000472"
     CDS             complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="OO369_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR5980863.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="OO369_000473"
     CDS             complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="OO369_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR5980864.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="OO369_000474"
     CDS             complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="OO369_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR5980865.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="OO369_000475"
     CDS             complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="OO369_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR5980866.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="OO369_000476"
     CDS             complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="OO369_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR5980867.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49715..50680
                     /locus_tag="OO369_000477"
     CDS             49715..50680
                     /locus_tag="OO369_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR5980868.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50694..51680
                     /gene="kdsD"
                     /locus_tag="OO369_000478"
     CDS             50694..51680
                     /gene="kdsD"
                     /locus_tag="OO369_000478"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR5980869.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51701..52267
                     /gene="kdsC"
                     /locus_tag="OO369_000479"
     CDS             51701..52267
                     /gene="kdsC"
                     /locus_tag="OO369_000479"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR5980870.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52264..52839
                     /gene="lptC"
                     /locus_tag="OO369_000480"
     CDS             52264..52839
                     /gene="lptC"
                     /locus_tag="OO369_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR5980871.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52808..53365
                     /gene="lptA"
                     /locus_tag="OO369_000481"
     CDS             52808..53365
                     /gene="lptA"
                     /locus_tag="OO369_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR5980872.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53372..54097
                     /gene="lptB"
                     /locus_tag="OO369_000482"
     CDS             53372..54097
                     /gene="lptB"
                     /locus_tag="OO369_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR5980873.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54145..55578
                     /gene="rpoN"
                     /locus_tag="OO369_000483"
     CDS             54145..55578
                     /gene="rpoN"
                     /locus_tag="OO369_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR5980874.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEETDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55601..55888
                     /gene="hpf"
                     /locus_tag="OO369_000484"
     CDS             55601..55888
                     /gene="hpf"
                     /locus_tag="OO369_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR5980875.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56006..56497
                     /gene="ptsN"
                     /locus_tag="OO369_000485"
     CDS             56006..56497
                     /gene="ptsN"
                     /locus_tag="OO369_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR5980876.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56543..57397
                     /gene="rapZ"
                     /locus_tag="OO369_000486"
     CDS             56543..57397
                     /gene="rapZ"
                     /locus_tag="OO369_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR5980877.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57394..57666
                     /gene="npr"
                     /locus_tag="OO369_000487"
     CDS             57394..57666
                     /gene="npr"
                     /locus_tag="OO369_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR5980878.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57879..58511
                     /gene="yrbL"
                     /locus_tag="OO369_000488"
     CDS             57879..58511
                     /gene="yrbL"
                     /locus_tag="OO369_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR5980879.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="OO369_000489"
                     /pseudo
     CDS             complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="OO369_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="OO369_000490"
     CDS             complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="OO369_000490"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR5980880.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="OO369_000491"
     CDS             complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="OO369_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR5980881.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62547..63476)
                     /locus_tag="OO369_000492"
     CDS             complement(62547..63476)
                     /locus_tag="OO369_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR5980882.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64151..68611
                     /gene="gltB"
                     /locus_tag="OO369_000493"
     CDS             64151..68611
                     /gene="gltB"
                     /locus_tag="OO369_000493"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR5980883.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68624..70042
                     /gene="gltD"
                     /locus_tag="OO369_000494"
     CDS             68624..70042
                     /gene="gltD"
                     /locus_tag="OO369_000494"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR5980884.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70226..70705
                     /locus_tag="OO369_000495"
     CDS             70226..70705
                     /locus_tag="OO369_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCR5980885.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        17315 a        19176 c        17560 g        16661 t
ORIGIN      
        1 tggttaccgc gtagcctggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa
       61 catgaaatgc gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa
      121 caaagtctgg atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct
      181 tatggaacct tcagtgccgg atacagttac ggtaatgaca gcagccaata caattacggc
      241 gttaccggcg gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac
      301 gcttttgcgc ttatcgatgc taacggggct tctggcgtga ggatacaaaa ctatccgggg
      361 attgctactg atccctttgg ctatgcagtg gttccttatc tcacgactta tcaggaaaac
      421 cgtctctcgg tagatactac gcagctgccc gataacgtcg atcttgaaca aacaacacag
      481 tttgtggtgc ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat
      541 cgcgtacttg ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc
      601 agcaacgatg atacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct
      661 gggatatcga gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa
      721 tgtcagtttg cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcaca
      781 gcgcagtgcc attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt
      841 gatatccaca tcctgcgctt atgcctcctc aggagggtgt ggagccgaca gcactagcgg
      901 tgcgacaaat tacagcagtg tggttgatga tgttacggtg aaccagacag ataacgtgac
      961 aggacgggag tttacctctg caacgctaag tagcactaac tggcaatacg cctgttcctg
     1021 ctctgcgggt aaggcagtta aacttgtcta tatggtcagc cccgtactta ccaccactgg
     1081 acatcagaca ggatattaca aactcaatga cagcctggat attaaaacca cattacaggc
     1141 aaacgacatt ccaggactca caaccgacca ggttgtctct gttaacaccc gattcacaca
     1201 gataaaaagc agcaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac
     1261 gtctcgttat ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa
     1321 gccatttctc ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcgtg
     1381 ggtcgatgga atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat
     1441 tcagggaaat ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaagt
     1501 taattttgga ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt
     1561 tactccagta gactttgata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc
     1621 gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag
     1681 gcgaagaagt tcagacaatg tgcccgatgt cggtattcgt attgaaaatc tcggcggcgg
     1741 agttgcaaat attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac
     1801 cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt gagctggaaa caggaaaatt
     1861 tcagggcaca gccaccatta ccgtcatggt gcggtaaaag aagacgttgc gttttgatta
     1921 acgcaacgct tcggcactgt tacccctgct gctccagcgc atacttatac agcgcatttt
     1981 tcttcacgcc atgaatttct gcggccagcg ccgccgcttt tttcagcggc agttctgcct
     2041 gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac
     2101 cttcgacaat cagcaccatt tcgcctttgc gacggttttc atcttccttt acccacgcca
     2161 gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca
     2221 gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac
     2281 ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct tttagcgcat
     2341 cacggcggcc ttttgatttg gcaggtaaaa agccttcgta acagaaacgg tcagatggta
     2401 aacccgcggc gcttaacgca gtgatagcag cacacggtcc gggtagcggc accacacgaa
     2461 tccccgcttc acggcaggta cgcaccagat ggtagccagg atcgttaatt agcggcgttc
     2521 cggcatcgga aaccagcgca atgttttgcc cctcttgcag ctttgccagc agcgtttcgg
     2581 ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc ccaaaatgtt
     2641 gcagcaataa accggtgtga cgagtatcct cggcggcaat cagatcaacg gcctgtaata
     2701 cctctaacgt acgctgggtg atatccgcca gattgccgat tggcgtcggt acaatgtaaa
     2761 gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc gtattgccga
     2821 tttaatattg agcattgcgt aaaaaaaata tcactggata cattatggta ccctcaacat
     2881 tttctcgttt gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg attttcgccg
     2941 gttgtggcac ccatactccc gatcagtcca ctgcttatat gcagggcacg gcgcaggctg
     3001 attctgcctt ttatcttcag cagatgcagc aaagctctga tgataccagg atcaactggc
     3061 aattactcgc cattcgtgca ctggtgaaag aaggtaaaac cgggcaggcg gttgagttgt
     3121 ttaaccaact accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca ctgctggcgg
     3181 cagagattaa actggcgcag aaagattttg ctggcgcgca aaacttgctg gcgaaaatca
     3241 cacctgccga tttagaacaa aaccagcaag cgcgttactg gcaggcaaaa atcgatgcca
     3301 gccaggggcg tccttccatt gatttactgc gcgcgttaat tgctcaggaa ccgctgcttg
     3361 gcgcgaaaga aaaacagcag aatattgatg ccacctggca ggcgctctcc tccatgactc
     3421 aggaacaggc gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa ggctggctgg
     3481 atctgcagcg cgtctggttt gataaccgta acgatcccga catgatgaaa gccgggatcg
     3541 ccgactggca gaaacgttat ccgaacaatc cgggcgcgaa aatgctgcca acgcagttgg
     3601 ttaacgtaaa agcgtttaaa ccagcctcga ccaacaaaat cgccctgctg ttgccgctga
     3661 atggccaggc agcggtattt ggtcgcacta ttcagcaagg ctttgaagcc gcgaaaaata
     3721 tcggcactca gccagtggcg gctcaggtag ctgccgcacc tgccgcagac gtagcagaac
     3781 aacctcagcc gcaaactgcg gatagcgttg ccagcccggc acaagcctcg gttagcgatc
     3841 tgaccggtga tcagcctgcg gcccagccgg tgcctgtaag cgccccggcg acaagcaccg
     3901 cagcggtaag cgcacccgca aatccatccg cagagctgaa aatctacgat acctcatcac
     3961 aaccacttag ccagatctta agccaggttc agcaggatgg cgcgagtatt gtggttggtc
     4021 cgttgctgaa aaataacgtt gaagagttgc tgaagagcaa caccccgctg aacgtactgg
     4081 cactgaacca gccggagaat atcgaaaacc gcgtcaatat ttgttacttc gcgctttcac
     4141 cggaagacga agcgcgcgat gcagcgcgtc atattcgtga ccagggtaaa caagcgccgc
     4201 tggtgctgat cccacgcagt gcattgggcg atcgcgtagc caatgcgttt gcgcaagagt
     4261 ggcagaaact gggcggcggc accgttctgc aacaaaaatt tggttccacc agcgaattac
     4321 gcgcgggtgt taacggcggt tctggtattg ctttaacggg tagcccgatt actcccagag
     4381 cgacaaccga ctccggcatg acgaccaaca atccaacgct gcaaaccacg ccaaccgatg
     4441 accagttcac caataatggc ggtcgtgtcg atgcggtcta cattgtggca acgccgggtg
     4501 aaatcgcttt tatcaaaccg atgatcgcca tgcgtaacgg tagccagagc ggtgcaacgc
     4561 tgtacgccag ttcccgcagc gcacaaggca ccgcaggccc ggatttccga ctggagatgg
     4621 aaggcttgca gtacagcgaa atcccgatgc tggcaggcgg taatctgccg ttaatgcagc
     4681 aggcgctcag cgcggtgaac aacgattatt cgctggctcg catgtatgcg atgggcgttg
     4741 atgcctggtc gttggcaaat catttctcac aaatgcgcca ggttcagggt tttgaaatca
     4801 acggtaatac cggaagtctg acggctaacc cggattgcgt gattaacagg aagttatcat
     4861 ggctacagta ccaacaaggt caggtagtcc ccgccagtta accaccaaac agaccggcga
     4921 tgcgtgggaa gcacaagcgc gtcgctggct ggaaggcaaa ggactgcggt ttatcgccgc
     4981 taacgtgaac gagcgtggcg gcgagatcga tctgataatg cgtgaaggcc tgaccaccgt
     5041 ttttgtcgag gtacgctatc gccgctctgc gctttatggc ggcgcggcag ccagtgtgac
     5101 ccgcagcaaa caacacaaat tattacagac tgcccgcttg tggctcgcgc gtcataatgg
     5161 gagttttgat actgtggatt gccggttcga tgtggtagcc ttcaccggga atgaggttga
     5221 gtggattaag gatgccttta atgaccactc ataattaagg tttaaggatt agcgtgcaag
     5281 aaagaattaa agcttgcttc actgaaagca ttcaaactca aattgcggcg gcagaggcgc
     5341 ttccggatgc catctcccgt gcagccatga cgctggttca gtctctgctc aatggcaaca
     5401 aaatcctctg ttgtggtaat ggaacttccg ctgccaatgc acagcatttt gctgccagca
     5461 tgatcaaccg tttcgaaacg gagcggccca gcttacctgc cattgcacta aatactgata
     5521 atgttgtctt aacggcgatt gccaacgatc gcttacatga tgaagtgtat gcaaaacagg
     5581 tgcgggcgct gggtcatgcg ggagatgtat tgttagccat ttccacccgt ggcaacagcc
     5641 gcgatattgt taaagcagtt gaagccgccg ttacgcgtga tatgaccatt gtggcattga
     5701 ccggctatga cggcggcgaa cttgcaggtt tgttagggcc acaggatgtg gagatccgca
     5761 ttccttcgca tcgtagtgct cgcattcagg aaatgcatat gctgacggta aattgcctgt
     5821 gcgatctgat cgataacacg cttttccctc accaggatga ttaaggagaa tacatgaagg
     5881 cattatcgcc aatcgcagtc cttatttccg cgctgctgtt gcaaggttgt gttgccgctg
     5941 ccgtagtggg taccgctgct gtgggtacca aagccgcaac tgacccacgc agtgtcggca
     6001 cccaggtgga cgatggtacc ctggaagtgc gcgtgaacag cgcattgtcg aaagacgaac
     6061 agattaagaa agaaacccgt attaatgtca cggcctatca gggcaaagtg ctgctggttg
     6121 ggcagtcacc aaatgctgaa ctttcggctc gcgccaaaca gattgctatg ggcgtagacg
     6181 gtgccaacga agtgtataac gagattcgtc agggccagcc gattggtctg ggcgaagcat
     6241 ctaacgatac gtggatcacc accaaagtgc gttcgcagct cttaaccagc gacctggtga
     6301 aatcgtccaa cgtgaaagtg accaccgaaa acggtgaagt gttcctgatg gggctggtga
     6361 ctgaacgtga agcgaaagcg gcggcagata ttgccagccg ggtgagcggc gtaaagcggg
     6421 ttaccacagc gtttacgttt attaaatagc agccccttgt aatgcctgat gcgacgcttg
     6481 ccgcgtctta tcaggcctac aacgatgcaa accgtaggtc ggataaggcg tttacgccgc
     6541 atccgacatg ttttccctca aatcagaaca acagcgccag cccgccgaca atcacaccag
     6601 acactgccac catcgcccct gtcagccata aggctttcgc cgggaacgct ttgcgcagca
     6661 taatcagtga cggcaaactc actgccggga gcgtcatcaa caatgccagc gccggagcgg
     6721 ttcccatacc tgccagcatc atcgtttgta caatcggaat ttctgctgcc gtgggaatga
     6781 caaacaagca tcctgctacc gccatcgcca ccacccacat caggctgtta tcgacagcac
     6841 catcggcatg ggggaataac cagacgcgtg cggcacccaa caccagtact gcaaggatgt
     6901 aaaccgggat cgtactccag aaaagtgtcc atagcgccct gccccagcgg ctaaaaaaac
     6961 cgctctgtgc ttccggtatg tcaatttcga ccggtgcctg cgtttgcggt gtttcacgca
     7021 cccatttttg caccagcgtc gcaatcagca acaccatcac cagcccggcc accagacgaa
     7081 tcgccgcaaa accccagccg aggacaaacc ccataaacac cagcgtcgcc gggtttaaca
     7141 ccggattgcc catccagaat gccagcgcac cgcccatcga tacctgttgg cgacgcattc
     7201 ccgccgcgac cggagccgca cagcaggtac acatcatgcc cggcagcgaa aacagcgttc
     7261 ccagcagcgt gccgcgaaag cgcgattgcc caagcgtacg caacaaccag tcacgcggga
     7321 tcagcacctg aatcaacgaa ccgaggatca cccccagcac cgccgctttc catactgcga
     7381 ggaaatagat catcgcgtaa tccaacgccg cttgccacgg attagcatcc gcctgcgcaa
     7441 ggatagattt accgatactg tgggtttcgg ctgcagtaaa cgctttgccg tagtaaggtt
     7501 gccatttcac ataccagaga ccggcaatga caacgagaaa gaaaagcgcg ggtttccacc
     7561 actgaatggg cgttgccgcc tgagatgaag actgaccagt catagcattc cccaggaata
     7621 agttatgata attgagcgcg tgaatattac gctcactatc aattcttggg aataattatt
     7681 cagctctttt gcgtaattct gaagagctta aaatcgtcac gccttcatgc tcaggccgca
     7741 tcgcctctgc cagcatgacc cgcgccacgt ctctcgcatc aatggatttc cagttacctg
     7801 gtaacaaacg gaacagcggc gcaaaaagcg tttcgttcat ccgctgtttg ctacgatcgc
     7861 ccagtaacat cgacgggcga gcaatggtca atttcggcca gttctgggca attaatgctt
     7921 cttccatctc ccctttgacg cggttataga aaaacggcga gtgggcattg gcacccatag
     7981 cactgaccac cagcatatgc tgcgcgccca gtcgccgccc ggttaatgcg gtatccacta
     8041 ccagcgtgta atcggcatga ataaacgcct ctttgctccc cgcttctcgc cgcgtggtgc
     8101 cgagacaaca aaacacaatg tcgatgggtt cggtgacctg cgccaacgcg tcactcagtt
     8161 gcggatcatg gggattaaac accccgggca tatcgcccaa cggacgtcgc gtcggcgcag
     8221 caatggcgtt aactttcggt tcattaatca acatccgcag caggtgaccg cccaccagcc
     8281 ccgttgcgcc tgtaatcagt acctgactca tcttcgttcc tttacagaat tgtctgcctt
     8341 gcgctccccg gctcatgcac cagtcttaat agaccatgag gtaattatcc ccgattgtgg
     8401 aaaattcgcc tcatccaatg caacaacgcg gaggaagcat gagtaagaaa attgccgttt
     8461 taatcactga tgaatttgaa gattcagaat ttacttcacc cgcagacgag ttccgtaaag
     8521 ccggacacga agtgattact attgaaaaac aagcgggtaa aacggtgaaa ggcaaaaaag
     8581 gagaagccag cgtgaccatc gataaatcca tcgatgaagt gacgccagcg gagtttgatg
     8641 ccctgttgct accgggtggt cattcaccgg attatctgcg tggcgacaac cgttttgtca
     8701 cctttacccg tgattttgtg aatagcggca aacctgtttt cgccatctgt cacggtccgc
     8761 agctgctgat cagcgccgat gtgattcggg ggcgcaaact gaccgcagtt aaaccgatca
     8821 ttattgatgt taaaaatgcg ggcgtggaat tttacgatca ggaagtcgtg gttgataaag
     8881 atcagctggt taccagccgg acaccggacg atctgccagc gtttaaccgc gaggcgttac
     8941 gcctgctcgg tgcctgagtc gcgtagccaa atcatttttt taccaaagcc cagcgtgttg
     9001 tcggtgaatt tgatttcatc gagccggatt tcccacaccg gggccgacag cattctggca
     9061 accggaaagc gacgattgta cgccttgcgc gcgaggtcgc tttcctcacc ttccagcctg
     9121 cggatctcac ctttaaactg cacaccgcga attaacgcta ccgttttcgg ctgaccgttt
     9181 accgttccgg caaccgcagc ctgcggccca ctcatctgcg cgtggcgcgt tttttcttcc
     9241 gtcaaaatgt agaaggcgac tttctgcgca tcaaaaagat aaaaggcatt agcgcaccag
     9301 agttcccctt cctgctgcac acaccaggtg acaacatgtt gttttgccag ccaacggctg
     9361 atggcgatga gtgtttccat tgctgttctc ttttatactg tgggcctgac cttaacatgc
     9421 gatgcagacg atgacaccct ggtttcttta cttgatccgt accgccgaca ataagcttta
     9481 taccgggatc accacggatg tcgaacgccg ctatcagcag caccaaagcg gcaaaggggc
     9541 gaaagcactg cgcgggaaag gagaactgac gctggcgttt tctgcgccag tgggcgaccg
     9601 ttcgctggca ctacgggcgg aatatcgcgt taagcaactg accaaacggc aaaaagagcg
     9661 tctggtagcg gaaggcgcag ggtttgcaga actgttgagc agcctgcaaa ccccggagat
     9721 taaaagcgat tgaaatgctc gtgatactca accagaccgg ttacgccatt cagcgcgtca
     9781 tccgctaaac gatgtaccag gaaggcgctt tcagtgcccg gccagcggca acgcagatcg
     9841 tgatgcgccg ccagttcaaa gccgaaacga ctgtacagcg ccggatcgcc cagcgtcacc
     9901 actgcggcat agccgaactc attaagcgaa tcgagtcctt catagaccag ttggcgtgcc
     9961 agcccctgtc cacggtattt ttcatcaacc gccaacggtg ccatgccgac ccattgcagg
    10021 tcttcgccct gcacatcaac cggactaaat gccacatagc caatgacctg accttcgtca
    10081 tctgtcgcca ccagccccag cgtcagaaag ccatcttcac gcagatcgtg aaccagcttc
    10141 gcttccgcat cactttcgaa tgagcgacgc agcagggcat caatacccgg cgcatcaatg
    10201 ggaatttcta ctcgaattag catggttcac ctaccgatgt ctgtttggtt tcaggcgcgg
    10261 ttttcattcc cgcctcaaca aaatccgcca gttgcagcag catcatgcgt aaggctttcg
    10321 gcatttgctc cagttcaatg gcgtccatca ggtttttcac atacaccccc agctccgtat
    10381 cgccttcaat caccagccga cgctggaaga agagcgtatc cggatcttgt ttacgcgccg
    10441 caatcatcag cagatcgctg gcgtcggcac taaaactcac atcagcttgc gtgttctggc
    10501 tgaccaccag tttgccattc accaccgagg taaaccattg caggtcaata tcacgcacat
    10561 gaatacttaa ccagcggcct tcaagaaact ccagctcgcc atcatccagc gcctgacgga
    10621 attgccagct taagacctgc tcaagaacct ggcgttttag cgcaaatggc gtcagtttta
    10681 ccggtacact caacagagat ggccccaaat gcacaatacg ggaacgcagt ttatccaaca
    10741 cgagttttac tccctgtttc aacaatcatc ctattttgcc atatcagaaa aataacatag
    10801 cggtataaat caacaattcc atatgaaatt gctgctacca ccaatacaac tttaactgcc
    10861 ttaaatcaaa aattgtcgca gcaaggttaa ctaaaatccc agttcgttaa catttttgcg
    10921 ttttgatagc gcaaccttca ggaaaaatta tggagctgct ctgccctgcc ggaaatctcc
    10981 cggcgcttaa ggcggccatc gaaaacggcg cagatgctgt ttatatcggg ctaaaagatg
    11041 ataccaatgc ccgtcacttc gccggcctta actttaccga gaaaaaattg caggaagcgg
    11101 tgagttttgt ccatcaacat cgccgcaaac ttcacatcgc gattaacact tttgcgcatc
    11161 cggacggtta cgcccgttgg cagcgtgccg tggatatggc ggcgcagctg ggtgccgacg
    11221 cgctgatcct cgccgacctc gccatgctgg agtacgccgc cgagcgttac ccgcatattg
    11281 aacgtcatgt gtcagtgcag gcttcggcga ccaatgaaga ggcgattaac ttttatcatc
    11341 gccattttga cgttgcccgc gtggtgctgc cgcgcgtgtt gtcgattcat caggtgaaac
    11401 agctggcacg ggttactcct gtcccgctgg aagtgtttgc tttcggcagc ctgtgtatta
    11461 tgtcggaagg ccgttgctat ctgtcgtcgt atctgacggg tgagtcgccc aacactgtgg
    11521 gtgcgtgttc tccggcccgt ttcgtgcgct ggcaacaaac gccgcagggg ctggaatccc
    11581 gcctgaacga agtgctgatc gaccgttatc aggacggcga aaacgcaggt tatccgacgc
    11641 tgtgtaaagg gcgttatctg gtggacggcg agcgctatca cgcgctggaa gaaccaacca
    11701 gtctcaatac cctggaactg ctgccggagt taatggctgc gaatattgct tcggtgaaaa
    11761 ttgaaggccg ccagcgtagc ccggcgtatg tcagccaggc ggcgaaagtc tggcgtcagg
    11821 ctatcgaccg ttgtaaggcc gatccgcaaa acttcgtacc gcaaagcgcg tggatggaga
    11881 cgctcgggtc gatgtccgaa ggcacgcaaa ccacccttgg cgcgtatcac cgtaaatggc
    11941 agtgagaaga gcaatgaaat attccttagg gccagtgctg tggtactggc caaaagagac
    12001 gctggaagat ttttatcagc aagccgccgc cagcagcgcc gacgtgattt atcttggtga
    12061 agcggtatgc agcaagcgcc gggcaaccaa agttggcgac tgtctggaga tggcaaaatc
    12121 gctcgccgga agtggcaagc agattgtgct ctccacgctg gcgctggtgc aggcatcctc
    12181 tgaactgggc gaactgaaac gctatgttga gaacggtgaa tttctgattg aagccagcga
    12241 tctcggcgtg gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg ggcacgcgct
    12301 gaactgctac aacgcggtga cgctgaaaat attgctcaaa cagggcatga tgcgctggtg
    12361 tatgccggtg gagctttccc gcgactggct ggtgaatctg cttaatcagt gcgatgagct
    12421 gggcattcgc aaccagtttg aagtggaagt cctgagctac ggtcatctac cgctggccta
    12481 ctccgcccgc tgctttaccg cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg
    12541 ctgcattaag tatccgaacg ggcgcaacgt gctgtcgcag gaaaaccaac aagtgtttgt
    12601 actcaatggc attcagacca tgagcggcta cgtttacaac ctcggtaacg agctggcatc
    12661 catgcaggga ttggttgatg tagtccgcct gtcaccgcag ggcactgaca ctttcgcgat
    12721 gctcgacgcc ttccgcgcta atgaaaatgg cgctgtgcca ctgccgttga cggcaaacag
    12781 cgactgtaac ggctactggc ggcggctggc aggactggag ctgcaagcct aagtaaatag
    12841 ctcactttgt taacaacttt aactattctt taatgcagta ttaaagatta atcggtaaca
    12901 aagtgagctg ttatgactga taaaaccatt gcgttttcgc tactcgatct ggcccccata
    12961 cccgaaggct cttcagcgcg ggaagcgttc tcccactctc tcgatctcgc ccgtctggct
    13021 gaaaagcgcg gctatcatcg ctactggctg gcagaacacc acaatatgac cggcattgcc
    13081 agtgctgcaa catcggtatt gattggctat ctggcggcga ataccaccac gctgcatctg
    13141 gggtctggcg gcgtgatgtt gcctaaccac ttaccgttgg tcattgccga acagttcggc
    13201 acactcaata cgctctatcc ggggcgaatc gatttggggc tgggtcgtgc tccgggtagt
    13261 gaccaacgga caatgatggc gctacgtcgt catatgagcg gcgatattga taatttcccc
    13321 cgcgatgtcg cggagctggt ggactggttt gacgcccgcg atcccaatcc gcatgtgcgc
    13381 ccggtaccag gctatggcga gaaaatcccc gtgtggttgt taggctccag cctttacagt
    13441 gcacaactgg cggcgcagct tggtctgcca tttgcgtttg cctcacactt cgcgccggat
    13501 atgttgttcc aggcgctgca tctttatcgc agtaacttca aaccgtcggc tcggctggaa
    13561 aaaccatacg cgatggtgtg catcaatatt atcgccgccg acagcaaccg cgacgctgaa
    13621 tttctgttta cctcaatgca gcaagccttt gtgaagctgc gccgtggcga aaccgggcaa
    13681 ctgccgccgc cgattcaaaa tatggatcag ttctggtcac cgtctgagca gtatgacgtg
    13741 cagcaggcgc tgagtatgtc gttggtaggc gataaagcga aagtgcgtca tggtttgcag
    13801 tcgatcctgc gcgaaaccga cgctgatgag attatggtta acgggcagat tttcgaccac
    13861 caggcgcgcc tgcattcgtt tgagctggcg atggatgtta aggaagagtt gttgggatag
    13921 tgtgtcttaa cgcgggaagc cttatccgag ctggcaacgc tgtcctacgt aggcctgata
    13981 agcgaagcgc atcaggcatt gtgtaggcag cagaaatgtc ggataaggca ccgctgatta
    14041 ctgatacacc ggcagtaaat taaagctcga taaaatatgc accagtgcgt tgccgacgcc
    14101 aaacaccaga atcagcgcaa tcatcggctt gccaccccag acgcggaatt tcgggctgcc
    14161 aaagcgttta cgcgatgcgc gggccagcag tgccggaaca attgccgccc agatggtagc
    14221 cgctaaacca gcataaccaa tggcatacag gaatccgttc gggaacagca gaccacccac
    14281 aacaggcggg gcaaaggtca gcaatgccgt tttcaagcgg cccacagccg agtcgtcgaa
    14341 accaaacaga tctgccagat agtcaaacaa acccagcgtt acgccaagga acgaactcgc
    14401 taccgcaaag tttgagaaca cgaccagcag aaggtccagg ctacggctgt tcagcacgcc
    14461 gcttaatgct tgtaccaaca catcaatatt accgcccttc tcggcgatgc cgataaactc
    14521 cggacgaggg atgttgccca ttgtcgccag caaccagatg gtatacagcg ccagtgccat
    14581 cagcgtaccg tacaccaggc atttcacgat ggttttcgga tctttgccgt aatacttcat
    14641 caggcttggc acgttaccgt gataaccaaa cgacgccagg cagaacggca gggtcatcag
    14701 caaatacggc gcataagatg cattgctttc ggcaacgttg aacaatgtcg caggctgcac
    14761 atgccccagc aggctaccga aggtgaggaa gaaggtaatg actttcgccc ccagcacaat
    14821 cgccgtcatg cggctgacgg ctttagtact caaccacacc acaaacgcta ccagcagtgc
    14881 aaaaccaaaa cccgccgccc gtgccgggac gtttagcgac atctctgcga aggtgtgatg
    14941 cagaatcgaa ccactggcag aaatataggc ataggtcagg atatagagca caaaggcaat
    15001 ggaaatgccg ttgaccacgt tccagccttt gcccagcaaa tctttggtga tggtgtcaaa
    15061 actcgaaccg attcggtaat tcaggttagc ttccagaatc atcaagccgg aatgcagcat
    15121 acagaaccag gtaaagatca gcgctgccat tgaccagaaa aaccacgccc cggacatgac
    15181 cactggcaga gaaaacatcc ctgcgccaat aatggtgccg ccgataatca ccacgccgcc
    15241 aagcagcgac ggtgacgttt gggtggtggt tagtgttgcc atgagggctt ctctccagtg
    15301 aaaaatagtg cgactgcgtt gttatgcatt gcactgtacc agtacatgag tacaaaagac
    15361 agaaaaaaag ccccgatggt taaaatcggg gctgtatata ttattttaca gattgtgttc
    15421 gctgttcagc gatgattacg catcaccacc gaaacgacga cgaccggtag agtcatcacg
    15481 acgcggtgcg cggccttcac gacgttcgcc gctaaaacga cgaccatcac cacgaccacc
    15541 ttcacggcgt tcaccgctga agttacgacc gccttcacga cgctcgccac cgaaaccacg
    15601 accaccgcca cgacgctcac cgccagtatg cggctgtgca tcgcccagta actgcatgtt
    15661 catcggcttg ttgagaatgc gagtgcgcgt aaagtgttgc agcacttcac ccggcatacc
    15721 tttcggcagt tcgatggtgg agtgagaagc aaacagcttg atgttaccga tgtaacggct
    15781 gctgatgtcg ccttcgttag caatcgcgcc aacgatatga cgaacttcaa caccatcatc
    15841 gcggcccact tcaatgcggt acagctgcat atcgccaaca tcacgacgtt cacgacgcgg
    15901 acgatcttca cggtcgccac gcgggccacg gtcgttacga tcgcgcggac cacggtcatc
    15961 acggtcacgg aattcacgtt tcggacgcat cggcgcatct ggcggaacaa tcagagtacg
    16021 ttcaccctgt gccattttca gcagtgccgc agccagagtt tcgagatcca gctcttcacc
    16081 ttctgcagtc ggctgaattt tgctcagcag tgcgcggtat tgatccagat cgctgctttc
    16141 cagctgctgc tgtactttag cggcgaattt ttccagacgg cgtttgccca gcagttctgc
    16201 gttcggcagt tctacttccg gaatagtcag cttcatagta cgttcaatgt tgcgcagcag
    16261 acgacgctcg cggttctcaa cgaacagcag cgcgcggcca gcacgacccg cacgaccggt
    16321 acgaccgata cggtgaacgt aagactcaga atccatcggg atatcgtagt taaccaccag
    16381 gctgatacgc tcaacgtcca ggccacgggc cgcaacgtcg gtcgcaatca ggatgtccag
    16441 acgaccgtct ttaaggcgtt ccagtgtctg ctcacgcagc gcctggttca tgtcaccgtt
    16501 cagcgcggcg ctattgtagc cgttacgctc aagagcttca gccacttcca gagtcgcgtt
    16561 tttggtacga acgaagataa tcgccgcatc aaaatcttcc gcttccagga aacgtaccag
    16621 tgcttcgttt ttacgcatac cccacacagt ccagtagctc tggctgatgt caggacgggt
    16681 agtcacgctg gactgaatac gcacttcctg cggctctttc ataaagcggc gggtaatgcg
    16741 acgaatcgct tccggcatgg ttgcagagaa cagagcggtc tgatgacctt ccgggatctg
    16801 cgccataatg gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt cagcttcatc
    16861 cagaaccaga ccgctcagtt tagagaggtc cagagtgcca cgtttcaggt ggtccagcag
    16921 acggcccgga gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta attgcacgtc
    16981 ataacgctgg ccgccgtaca gggcaaccac atttacgccg cgcatgtgtt tagagaaatc
    17041 cgtcattgct tcagcaacct gtaccgccag ttcgcgggtc ggtgccagca ccagaatctg
    17101 tggtgctttc agctcaggat caagattctg cagcagaggt aaagagaatg ctgcagtttt
    17161 tccgctcccc gtctgggcca tacccagaac gtcgcggcca ttcagcagat gtggaataca
    17221 ctctgcctga attggagatg gtttttcgta acccagatcg ttaagggctt caaggatagg
    17281 agccttcagg cccagatctg caaaagtggt ttcgaattca gccatgtagt acgtgtgcct
    17341 caaaattaat ggcggccagt ctacataact catcatgaaa ttgatcagca attttcattg
    17401 aaaagtgtga accggctcaa agtaggtgta ttaacgaaca acaacgccct cacccgttaa
    17461 ggtgatggca atcaaaaaag attacgggct gatgtgtacg tcagctattg ctggtccgat
    17521 tctgccaggt catcttggtc ctggcccagg agcgataatt ccaacaatgc gtatcggtgc
    17581 tcaacaaagt tatgaacgtt gttggcaacc gccagtttga acagtgccgt ggcgctgtcc
    17641 aaatccccca gacttaggta gtacttacct aaatagaagt tggtttcact gagatgctca
    17701 gcgagcgagg tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt ttgttcgcta
    17761 atgttgccca ggtagaactc gacaatgttc catccccact gttccttatc cgatttttcg
    17821 aagtgctgtt tcaatacttc tttagcctgc ttctcatcga gcttctgctc ggcgagataa
    17881 agccacagac tacggaaagg atcattggga tcgtcttgat aaaacgccag cagatcatct
    17941 tgcgctaact tgtcacgacc gccgtaatat aatgcaatcc cgcgattcaa gtgcgcgtag
    18001 ttgtaagttg gatcaagctc aagtacagaa tcaaacgctt cataggcagc atcaaaattg
    18061 cctgcctgcg ttaaatatat gcctaagtaa ttgaatactt caggcatatc cggtcggatt
    18121 gccagcgctt gcgaaaaatc gttacgcgct aatgccctca gaccgagact atcatacaac
    18181 actccgcgct catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg actggcaagg
    18241 atttgttcca tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa tggtaccgcg
    18301 aggacttcac ttttacgcca ggaagtatta ctgcatcctg caagcgtaag tgctgtcgca
    18361 acgaaacacc agcgcaaaaa aggcttcatt tcccactccc gaagaccacg gttgaatgaa
    18421 cgtcctgttc ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc cgccgcagcg
    18481 gagggcaaat ggcaacctta ctcgccctgt tcagcagccg gagcttccgg tgctgcagca
    18541 ggttgagact gctcagtcgc ttctttaatg ctcagacgga tacggccctg gcgatcaact
    18601 tccagaactt tcaccggtac ttcctgaccc atctgcaggt aatcggtcac tttctcaacg
    18661 cgtttgtcag cgatttgaga gatgtgtacc agaccttctt taccgccgcc gatggcaaca
    18721 aatgcgccaa agtcaacgat acgggtcact ttaccagtgt agacgcggcc cacttcgatt
    18781 tctgcagtga tctcttcgat acgacgaata gcatgtttcg ctttctcgcc gtcggtcgct
    18841 gcaatcttca cagtaccgtc atcttcgatt tcgatggtgg tgccagtttc ttcggtcagg
    18901 gcacggataa cagagccgcc tttaccgata acgtctttga tcttgtccgg gttgatcttg
    18961 atggtatgga tacgtggtgc gaactcagag atatcgccac gcggcgcgtt gatcgcctgt
    19021 tccattacgc ccaggatatg cagacgcgca cctttagcct ggttcagcgc aacctgcatg
    19081 atctctttgg tgataccttc aattttgata tccatctgca atgcagagat accctcgcgg
    19141 gaacccgcaa ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa aatgtcagac
    19201 agtacaacgt agttgtcgcc ttctttcacc agacccattg cgatacccgc aacggcagct
    19261 ttgatcggca cacctgcgtc catcagcgcc agagacgcgc cgcacacgga agccatagaa
    19321 gaggaaccgt tggattcagt gatttcagac acaacacgta cggtgtacgg gaatttgtcc
    19381 atatccggca tgactgccag cacgccgcgc ttcgccagac gaccgtgacc aatttcacga
    19441 cgcttcggag aaccgaccat gccggtttcg cctacggagt acggagggaa gttgtagtgg
    19501 aacaggaagg tatcggtacg ttcgcccatc agttcatcaa gaacctgcgc gtcacgagca
    19561 gtacccagcg ttgcggtaac cagcgcctgc gtttcaccac gggtgaacag cgcagaaccg
    19621 tgagtacgcg gcagcacgcc agtacgcaca tccagaccac ggatcatatc tttttcacga
    19681 ccgtcgatac gcggttcgcc tgccagtacg cggctacgaa caacgttttt ctcgatcgcg
    19741 tgcagaattt cacccagttc gttttcgtcc agggtttcgt cttcagcaag cagcgtcgcg
    19801 atggtttcag atttgatgac atcaacctgc gcataacgct cttgtttgtc ggtgatgcgg
    19861 taagcatcgc tcaggcgagc ttcagccagt gcagcaacgc gcgcgttcag cgcttcgttt
    19921 accggctccg gctgccagtc ccaacgtggt ttaccggctt ctttcaccag ttcattgatg
    19981 ttctgaataa caacctgctg ttgttcatga ccgaacacta ctgcgcccag catctggtct
    20041 tcgctcagca gttcagcttc agattcaacc atcagtacag cggcttcagt accggcaaca
    20101 accagatcca gtttgctctc tttcaactcg tcctgagtcg ggttcagtac gtactggtca
    20161 ttgatgtaac ctacgcgagc agcaccaatc ggaccattga acggaatacc agacagagac
    20221 agtgctgcgg aagcaccaat catcgcgacg atatccgggt taacttgcgg gttaacagaa
    20281 accacggtgg cgataacctg aacttcgttg acgaagcctt ccgggaacag cggccgaatc
    20341 gggcggtcaa tcagacgcgc gatcagggtt tcgccttcgc ttgggcggcc ttcacgacgg
    20401 aagaagctac ccgggatacg accagcagcg taggtacgct cctgatagtt aacggtcagt
    20461 gggaagaagt cctgacctgg tttggctttt ttctggccaa caacggtaac aaataccgcg
    20521 gtgtcatcca tgctaaccat aacagcggca gtagcctgac gagccatcat gccggtttcc
    20581 agagtcacgg tgtgttggcc gtactggaat ttacgaacga tcggattaag caatgtaata
    20641 tcctttctct ttcttagaca gtaccttacg gcactggtgt taatacccga tcttctgcgc
    20701 atcctcgcga ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct ctcattagcc
    20761 gcgcgaacct ctgcaacgga agatcattca tagcaacaat acattagttt ccagtgaatt
    20821 gctgccgtca gcttgaaaaa aggggccact caggccccct tttctgaaac tcgcaagaat
    20881 tagcgacgca gacccaggcg ctcgatgagc tgggtgtaac gtgctacgtc tttacgtttc
    20941 aggtagtcga gcagtttacg acgctgagaa accatgcgca gcagaccacg acggctgtgg
    21001 tgatcttttt tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc agtcagcagt
    21061 gctacctgaa cttcagtaga accggtgtcg tttgcgtcac gaccaaactc agaaacgatt
    21121 ttagctgttg cttcagtact tagagacatt ttaaaactcc aaagtatata gaatgaaagg
    21181 acgccgatct ctaattcagc gatcccagtg tacgttacgc aaagtgttaa acaatttacg
    21241 cgacgttaag cggcagtatt ctactcgtag cgacctgtta tcgcaagacg gttaacatta
    21301 cgccgggtat tcaaccacca ggcgacgagg cgcaacgcgg ccttcatcgt caatttcgcc
    21361 cataccgata aatttgccgt tctcaccttc tgtaacgcga accagtcctt ccagcggcgc
    21421 accggatgta cgaactgggt taccattttt gaagtaaaca gaagacgtta acggaagatt
    21481 caccaccggg tagtccgaag ctggactgtc cattggcatc agtaatggat caagtaactc
    21541 cgcagccgga atatcctgct gttcggcttg ttcgaccagt tcacgcagat gttccagggt
    21601 cacaatccgt tcaaccggat atttacttac cgccagacga cgcaggtaaa taacatgcgc
    21661 gccacaaccg agtttttcac ccaggtcatc aatgatggtg cggatataag tgccttttga
    21721 gcagtgaatt tccagctcca gctcattgcc ttcgtggcga ataaacagca attcataaac
    21781 ggtaatcggg cgcgcttcac gcggtacttc aatgccctga cgcgcatatt cgtacagttt
    21841 tttaccctga tatttcagtg ctgaatacat cgaagggatc tgttcgatat cgccacggaa
    21901 agtatccagc gccgccgcca gttgctctgc gctaaaggtt accggacgtt cttcaacgat
    21961 ttgtccgtcg gcatcagaag tatcggtacg ctgtccaaga cgcgcaatga cccgatagcg
    22021 tttgtcggag tccagcagat actgggaaaa cttcgtcgct tccccgaggc aaattggcaa
    22081 catgccagtt gccagcgggt ccagcgcacc ggtatgcccg gcacggttgg cgttataaat
    22141 ccgtttcact ttttgcagcg catcattgct ggacataccc tgcggtttat ccagcaacaa
    22201 aacgccgtta atgtcgcgac cgcgacgacg aggacgactc attagtcctc cttgctgtcg
    22261 tccgggttaa cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag gtttgacatg
    22321 cgcatccctt caaccagaga gttgtcgtag aagaaggtca gttccggcac gatacgcagg
    22381 cgcatcgctt tccccagcag gctgcggatg aaaccagaag cttcttgcaa cgctttgatg
    22441 cccgctttaa ccgcgtcttc atctttgtcg ttgaggaacg tcacatatac tttggcatac
    22501 gccaggtcgc gagacatttc gacaccggaa acggtggtca tcatgcccag gcgaggatct
    22561 ttaatttcac gctgcaggat gagagcgatc tctttttgca tttcctgcgc tacgcgctgc
    22621 gggcgaccaa attctttcgc cataataaat tctcctgaca aaaaaggggc tgttagcccc
    22681 tttttaaaat taatttcagg tggaagggct gttcacgttg gcctgataag acgcgccagc
    22741 gtcacatcag gcaatccatg ccggatgcgg cgtaaacgcc ttatcccgca tggaacccta
    22801 aaaaccttaa gcgatggtac gttggatctc gatgatttcg aatacttcga tcacatcgcc
    22861 agtgcggacg tcgttgtagt tcttaacgcc gataccacat tccataccgt tacggacttc
    22921 gttaacgtca tctttgaagc ggcgcaggga ctccagctcg ccttcgtaga taaccacgtt
    22981 gtcacgcaga acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa ccatacagcc
    23041 tgcgatggca ccaaatttcg gcgatttgaa cacgtcacga acttccgcca gaccgataat
    23101 ctgctgtttc agttccggag acagcatacc gctcatcgcc gctttcactt cgtcaatcag
    23161 gttatagatg acggagtagt aacgcagatc caggctttcc gcttcaatca ctttacgtgc
    23221 agaggcatca gcacgtacgt tgaagccaac caggatggcg ttggacgccg cagccagggt
    23281 ggcgtcggtt tcggtgatac cacctacgcc agaaccgatg atcttcactt taacttcgtc
    23341 agtagacagt ttcagcaagg agtcggagat cgcttccaca gaaccctgta cgtctgcctt
    23401 caggacgatg ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca tgttctcgag
    23461 tttagatttc tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac gatacagtgc
    23521 aacttcacgc gctttcttct cgtcacgtac aacggtaact tcatcacccg cagccggtac
    23581 gccggacagg ccgaggattt ccaccggaat ggacggaccc gcttccagca cttcctgacc
    23641 cagttcgtta cgcatcgcac gaacacgacc gtattcgaag ccacacagaa cgatatcgcc
    23701 cttgtgcaga gtaccttcac gtaccagaac ggtagcaacc ggaccacgac ctttatcgag
    23761 gaaggattcg ataaccgcac cgctcgccat acctttacgt accgctttca gctccagaac
    23821 ttccgcctgc agcaggatag cgtccagcag ttcatcgata ccagtacccg ctttcgcaga
    23881 cacatgtacg aactggcttt caccgcccca ctcttccggc aggatgccgt actgggagag
    23941 ttcgttctta acgcgatccg gatcggcttc tggtttatcg atcttgttca ctgcaacgac
    24001 caccggaacc tgcgccgctt tcgcgtgctg gattgcttcg atcgtctgcg gcattacgcc
    24061 gtcgtcggca gcaacaacca ggactacgat gtccgttgcc tgcgcaccac gagcacgcat
    24121 tgaagtaaac gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt tttcagtttc
    24181 aacgtggtat gcaccaatgt gctgggtaat gccgcccgct tcgccagagg ccactttcgt
    24241 tgaacgaatg tagtccagca gagaggtttt accgtggtca acgtgaccca tgatggtcac
    24301 aaccggcgcg cgcggttcag ccgcagcacc cgtgtcacgg tcgctcatta ccgcctcttc
    24361 cagctcgttt tcacgacgca ggataacttt atggcccatc tcttcagcaa ccagctgtgc
    24421 ggtttcctga tcgataacct ggttgatggt tgccattgcg cccagtttca tcatcgcttt
    24481 gatgacctga gagcctttaa ccgccatctt gttcgccagt tcgccaacgg tgatagtttc
    24541 gccgatcaca acgtcacggt taacggcctg agcaggcttc tggaagcctt gctgcagcga
    24601 agaaccttta cgttttccgc ctttaccgcc acgtactgct gcgcgtgctt cttcacgatc
    24661 agcttttgat tcagcgtgtt tgttgccttt cttcggacgc gctgctttcg cgttacgacc
    24721 acggccacgg ccgccttcga cttcacgatc gctttcgtct tctgcctggc gagcatgttg
    24781 agaagtagtg acgtgataat cgctggaatc ttcagtcggt tccgcgttat cagtccattt
    24841 gttttcttcc gccatacgac gtgcttcttc agcaacgcga cgtgcttctt cttcgagttt
    24901 acgacgcgct tcttcttcag ctttacgctt gagctctgca gcttcctgct cacggcgggc
    24961 tttttcagcc tgggcgtttt tagtcatatc gtcttgttga ttgctcactt tgtctttttc
    25021 cgcagcttca cgtttcgctt gttcagcagc ttcacgctta gcttgttctg cggcctcacg
    25081 ttcagctttt tgttgcgcct cgcgtttagc cgattcttct gcctcacgac gggcttgctc
    25141 ttccgctgca aggcgttcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg
    25201 gacttcgatt tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg
    25261 tgttttacgt tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat
    25321 caaagtctgt ttctcttgtg cagacacaga gtcgtcagca gacttccgga tacctgcatc
    25381 agcaaattgc tgtaccaggc gttccacgga ggtctgtcgc tctgcggcca gcgttttaat
    25441 cgttacatct gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca
    25501 gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca acccttcgat
    25561 atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc
    25621 ggccagtttg aatgccaaat cacgatctac cccttcaagg ttcagcagat cgtcagccgg
    25681 tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc
    25741 acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt
    25801 catcggcaca taggccagtt cttccagcgt cgagaaacct tcttctacca gaacagtcgc
    25861 gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc
    25921 ctgatgctta gcctgcaggt cgtcaacggt cattacgttg agttcccaac cgctcagctg
    25981 cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccagcttc
    26041 aacggcgata tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg
    26101 tgccattgcg ttaatcacaa actgcgccgg gttatcatcc cacaggacga tatcgatacg
    26161 ctcgccgccc agttcagtag aaaccgcctg aacacgcgcg ccacgcatac ctacgcaagc
    26221 acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac gagaacccgg
    26281 gtcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttccggca cttcaatacg
    26341 gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg cgccacgcgc
    26401 ttccgggcga acggaataaa gcacgccacg aacgcggtcg ccagggcgga agttttcacg
    26461 cggcagcata tcttcgcgca ggatcacggc ttcagcgttg ttgcccagat ccagagagat
    26521 gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa
    26581 ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc
    26641 cgtctgggtg gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc
    26701 caagttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg
    26761 ggtgacttca tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc
    26821 gatctgtaca cggacgtcga tctcttgctc atattttttc tttgttgctg tcgccagcgc
    26881 gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc
    26941 aactacagcc aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa
    27001 gtggggaacc aggttcgcct tctggatatt actcagcgcg aacacttcat ctttaccttc
    27061 gacggtaact gtgatcattt caccgtctac cgctttgata acgccctgcc atttacgacg
    27121 gttttgtacc gccatacgga gaaccagagt cacctcttct ccgacaaaac gggcgtagtg
    27181 ttcagccgtg aacagtgggc gatcgagacc cggtgaggag acttccaggt tataagcaac
    27241 ggtgatggga tcttcaacat ccagcacagc acttacctgg tggctcacat cagcacaatc
    27301 atcaacattg atgccatctt cactatcaat atagatgcgc agtgtggatg tgcgaccgcg
    27361 aataaattcg atgcctacca gttcaaaacc cagggcctca actggcgcag taatcatctc
    27421 tgttaatttt tgctctaatg tggacaagcc cacccccaag acataaaaaa agggcctaaa
    27481 gcccagttat tctgtagtca gataacaaaa aaccccgata aatcggggct ttatataact
    27541 gaaccctata accgcaactg cggtctggag cactttccag aaggattttt tcaaatccca
    27601 ctacgaaggc cgaagtcttc acagtatatt tgaaaaagga ctctaaggga aagtggttgc
    27661 gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaggctgct
    27721 ccaccccgcg cctgaaacgt ggcaaattct actcgttttg ggtaaaaaat gcaaatactg
    27781 ctgggatttg gtgtaccgag acgggacgta aaatctgcag gcattatagt gatccatgcc
    27841 acattttgtc aacgtttatt gctaatcacg tgaatgaata tccagttcac tttcatttgt
    27901 tgaatacttt tgccttcccc tgctctccct taagcgcatt attttacaaa aaacacacta
    27961 aactcttcct gtctccgata aaagatgatt aaatgaaaac tcatttattt tgcataaaaa
    28021 ttcagtgaga gcggaaatcc aggctcatca tcagttaatt aagcagggtg ttattttatg
    28081 acgacgattc tcaagcatct cccggtaggt caacgtattg gtatcgcttt ttccggcggt
    28141 ctggacacca gtgccgcact gctgtggatg cgacaaaagg gagcggttcc ttatgcatat
    28201 actgcaaacc tgggccagcc agacgaagag gattatgatg cgatccctcg tcgtgccatg
    28261 gaatacggcg cggagaacgc acgtctgatc gactgccgca aacaactggt ggccgaaggt
    28321 attgccgcta ttcagtgtgg cgcatttcat aacaccaccg gcggcctgac ctatttcaac
    28381 acgacgccgc tgggccgcgc cgtgactggt accatgctgg ttgctgcgat gaaagaagat
    28441 ggcgtgaata tctggggtga cggtagcacc tataaaggaa acgatatcga acgtttctat
    28501 cgttatggtc tgctgaccaa tgctgaactg cagatttaca aaccgtggct tgatactgac
    28561 tttattgatg aactgggcgg ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc
    28621 gactacaaaa tgtctgtcga aaaagcctac tccacagact ccaacatgct tggtgcaacg
    28681 catgaagcga aggatctgga atacctcaac tccagcgtca aaatcgtcaa cccgattatg
    28741 ggcgtgaaat tctgggatga gagcgtgaag atcccggcag aagaagtcac agtacgcttt
    28801 gagcaaggtc atccggtggc gctgaacggt aaaaccttta gcgacgacgt agaaatgatg
    28861 ctggaagcta accgcatcgg cggtcgtcac ggcctgggca tgagcgacca gattgagaac
    28921 cgtatcatcg aagcgaaaag ccgtggtatt tacgaagctc cggggatggc actgctgcac
    28981 attgcgtatg aacgtctgtt gaccggtatt cacaacgaag acaccattga gcagtatcac
    29041 gcgcatggtc gtcagttggg ccgtctgctg taccaggggc gttggtttga ctcccaggcg
    29101 ctgatgctgc gtgactctct gcaacgctgg gttgccagcc agatcactgg tgaagttacc
    29161 ctggagctgc gccgtgggaa cgattattca atcctgaata ccgtctcaga gaacctgacc
    29221 tacaagccag agcgtctgac gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt
    29281 attggtcaat tgaccatgcg taatctggat atcactgata cccgagagaa actttttggt
    29341 tatgccaaaa ctggcctgct ttcctcctct gccacttcag gcctgccgca ggtggagaat
    29401 atggaaaaca aaggccagta attcgacatc aaccctgccc ttcaggggtg gggttacctt
    29461 gcttatcctc tgtaaactcc tttctgagca tcacataatc gaaaacatca tggtttgcat
    29521 ccacaacccg tgagtctcct ttatagtgtg caatcacttc ctcaagcgtt tgcggctgtt
    29581 ccggctttgt tacccccatc cacgcattaa taaggtaatt attgtaagct gtcgaaaaga
    29641 tattgttttc cgttgtgcga tccacgccat ccccaagaac ggggctatac cagataaaca
    29701 tcgggacatg atatgcctgc tggctagcct ccctgcctcc atgaaaatag acgttcttct
    29761 tcgtagggtc acgttccagg ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt
    29821 ttaataattc aaaaacctga cctagcaaac tatctgtgta atgaatggag ttgtcatagc
    29881 aggcatcctg atcgtcctgc ggttgaaaca cggcgctgga ttgcggatag gcgctacaag
    29941 ccggttcatg gcttccattt aaatgaagaa caatcagttt cttttgctgc gtattttgct
    30001 gtaatgcttg cgataaatgc ggcaacaaca attcatcaaa tcctctgaca taaactgttt
    30061 ccatggcgcg catggcgata ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg
    30121 agcttagcca gaaagtctga aatcctgctt gattagccat attaataatg ttgtcggggt
    30181 aattatgaat gtcatgactc aaaacagaat cagcagttaa agaaagggga accgacagcg
    30241 cagtgtaagg tgcgccgctt attgcttgat taaacagttt gatctgtttt ctttgtgctt
    30301 caacttgcgg tgtcgtagag cgtgtatatc catacaaaga catattgtcg acacgtacag
    30361 actcccccac aatcaacacg taggtatcaa tacttgtatc cctgactgat aattgaaaat
    30421 acggcaccgt gtttgcaatt gagagtaatc tttgatgcgc tttcgctgct aaagcaaaat
    30481 agttgagatt gaaaaacggc gtataggtag caaatcacga cgctagtata tatggactga
    30541 acgctttttt attttttgca tctttataag caaattgaca agcggaaaat aaactgcccg
    30601 aaatgacaat cagcaataat attcctgtca ctttttttgt cggcaaggaa acatcatatt
    30661 ttattattac tgccaaaaaa agaagggata aaaaagcaaa agcacataga taagggatat
    30721 acatccccag cattttcaca acttcatccg gatcactctg cagcacacta atcgcaaatc
    30781 catcattgaa agttgtacca aaggtacacc atgaatagag gctaatactc atgtctgccg
    30841 cagtcagtaa cacaaatggt gcggcaacaa gcaggcgaag cggaagggat gatatacgtt
    30901 taacaaccag gatggtcagg acgaagaaca gcaatcgctg aaccatatgc gctccggaag
    30961 aggcgactaa gttcgtaata ccaaaaacaa taacacaaag atacaacaac caatgagatt
    31021 taaaatcttc tagcaaattt gttgaatact gtcatccaga aatccaagtt cacaaccaat
    31081 ataaacaatt atcaacaatg ttcaatttta tatcagacat gtttttcaac tctgatccat
    31141 atcagaccga aacactgtta gttttttctt tttaaatgat gcgaccattg cgttctcagt
    31201 aattaaataa cattatcaat gcgtctctaa acattacgca gaacgtagcc atcagtagtt
    31261 atactgacag gagcaggatg gtttatcgag aagtaaacat atccgtattc gtaccggaac
    31321 tggagttgac acaataaagt gccaattatg tcagcagtag ggaaaagttt ataactaagg
    31381 cgtaccggca ccatcgtttc aaggtaccag ctacgagtaa agcaactgga cgagatacag
    31441 atacctgaca accattcatc agaccaggac caaaacgaaa agagacgctt ttcagcgtct
    31501 cttttctgga atttggtacc gaggacggga cttgaacccg taagccctat tgggcactac
    31561 cacctcaagg tagcgtgtct accaattcca ccacctcggc acggatacta ctttttagtt
    31621 cgggatatcg ctggtcggct tagccggagc agctggctga gtttgttcgg ttttcgccgg
    31681 tgcactcaga ttttcccatt cgctacctct attggttttg ttgctattga tgttacccag
    31741 caccagactg atgatgaaga ataacgttgc cagcagcgcc gtcatgcggg tcatgaagtt
    31801 accagaacca cttgaaccaa acagcgtagc ggaagcgcct gctccgaagg aggctcccat
    31861 atcagcgcct ttaccttgct gcagcatgat cagaccaaca aggccaattg ccacaataag
    31921 gaaaactact aaaagagctt cgtacataat caacctgttc cttgcggagt tgccgcgtac
    31981 caatgcttca accaataaag agggagtttt taatctttcc cactgaagcg ggtgtgaata
    32041 ctaaccaaaa cgaatgacct tcgcaagggc aattttatcg cattgtatca actgcggaaa
    32101 aaaacagcaa aaccgatgtg ttcgccgaga aaaaaggcga caatcgccgc ctttttagcc
    32161 agttatctaa cgctttaaac ggcttttact gcatcggcga tgcggtgtgc aaattcagtc
    32221 acctgcgctt cgtcttcgcc ttccaccatc acgcgaatta acggttcggt gccggattta
    32281 cgcagcaaca cgcgtccacg gttgcccagc gcagcttcaa cctctgcggt cacggcttta
    32341 actgactcat gctcaagcgg atcgccgcta cctgcggtgt aacgtacgtt aaccagaatc
    32401 tgcgggaaca ttttcatgcc gctgcaaagg tcgtgcaggc tcatatggtt acgtgccatc
    32461 gccgccagca cctgcaagcc agcaacgatg ccgtcaccgg tagtggtttt atccagcagg
    32521 atcacatgac cggaattctc tgcaccgata cgccagcctt tctcctgcat tttttccagt
    32581 acgtagcggt cacccacttt cgcgcgcgca aatggaattc ctaactgttt cagcgccagt
    32641 tcaagcccca tgttgctcat caatgtaccc acagcaccac cacgcagctg gccctgacga
    32701 agaccttcac gcgcgatgat atacatgatc tgatcgccat cgactttatt gccttcatgg
    32761 tcaaccataa tcacgcgatc gccgtcgccg tcgaaggcaa taccgagatc cgctttttca
    32821 gccagcacac gagcctggag cgcgcgaacg tcggtagccc ccacttcggc attgatgttt
    32881 acaccgtttg gctcacaacc gatagcgata acgttcgccc ccagttcgcg cagcacgttc
    32941 ggcgcgatgt gataagtcgc accgtttgca caatccacca caatcttcag ttcactgagg
    33001 ctaagttcgt tcgggaacgt ggctttgcaa aactcgatat agcgacccgc ggcatcaacg
    33061 atacggctgg ctttacccag ttcagccgaa tcaacgcagc tgatctcctt ttccatttcc
    33121 gcttcgatgg cctcttctac cgcatccggc agtttagtgc cgtcgataga gaagaattta
    33181 atcccattat cgtagaacgg gttatgcgat gcagatatca caattccggc ctctgcgcgg
    33241 aaggtacgcg tcagataagc cacggccggt gttggcatcg ggccagtgaa gagtgcggaa
    33301 aggcccgctg ccgccagacc cgcttccagt gctgactcca gcatatagcc agaaatacgc
    33361 gtgtctttac caataataat cttacgggag ccgtggcgcg ccagcacttt acccgcggcc
    33421 caacccagct taagcacaaa atcaggtgtg atcggcgcat cccctacacg accacgaatc
    33481 ccatcggtac cgaaatattt acgattactc atagcgtttg ttttcctttg cagacagagt
    33541 ggcttccacc acccgcatcg cttctacggt ttctttgacg tcatgaacac gaatgatgtg
    33601 cgcgccttgc attgcggcaa tgaccgcaca ggccagacta ccgctcaggc gctcggacgg
    33661 ccccacgttc agcagctgcc caatcatcga ttttcgtgac atacccacca acagcggcag
    33721 gttgaaatgg tgaaattcag ccaggcgcgc cagtaatgaa tagttatggg agagattttt
    33781 accgaaaccg aatccggggt cgagcaacaa tttctctttt gcgatacccg cctgctcgca
    33841 acgtgctatt tgctcaataa agtagcgatt cacttctgca aagacatcgt catacttcgg
    33901 agcttcctgc atggtttttg gatttccctg catatgcatc agacaaaccg gtaaaccggt
    33961 ttctgcagcc gcctccagag cgccaggttc ggaaagggag cggatatcat taataatgtg
    34021 agcgccaact ttcgctgact cacggatgac ttctggtttg gatgtatcga ccgagatcca
    34081 gacttcgaag cgttgagcaa ttgcctcaac cacaggaata acacgttgca actcttcttc
    34141 aacgctaact tccgccgccc ctgggcgcgt ggactcgcca ccaacgtcaa tgatcgtcgc
    34201 gccagcattg atcatcagat tcgcatgttt caccgcatct atcagcgagt tatgcgtgcc
    34261 accatccgaa aaggaatcag gcgtgacgtt gaggatcccc attacgtgag gatggctaag
    34321 gtccagtgaa gtaccctggg caaagagttt cattatgtta tccctggtat gaattgataa
    34381 gaaaaaaccc cggagcacgc cccggggttt tcggtacaaa tacagtcatc tgatgcggga
    34441 acttacttgt cgcctaactg ctctgacatg gtgttacccg ggttcggcgt acgcggttca
    34501 tcaaccggac gaggagcctt tggactacca ttgtcgccag aattgttaga agcgcctggt
    34561 tcttcccagc ccgctggcgg acgtacatcg cgacgtgcca tcaggtcatc aatctgcggt
    34621 gcgtcgatag tctcatattt catgagagca tctttcatcg catgcagaat atccatattg
    34681 tcggtcagaa gctgacgcgc acgattatag ttacgctcaa tcagtgcttt cacttcctgg
    34741 tcgatgatac gtgcagtttc atcggacata tgtttcgctt tcgctacgct acggccgagg
    34801 aatacttcac cttcttcttc cgcgtacagc agtggaccca atttctcaga gaagccccac
    34861 tgagtcacca tgttacgtgc caggttggtc gcaactttaa tatcgttgga cgcaccggta
    34921 gaaacatgtt ccggcccgta gatgatctct tctgccagac gaccaccgta cagcgtagaa
    34981 atctggcttt ccagtttctg acggctggcg ctgattgcgt cgccctcagg caagaagaaa
    35041 gtcacaccca gcgcacgacc gcgtgggata atcgtcactt tatgcaccgg atcgtgttcc
    35101 ggcaccaggc gaccgataat cgcatgaccc gcttcgtggt aagccgttga ttctttctgc
    35161 gcttccgtca tcaccatgga gcgacgttcc gcacccatca tgattttgtc tttcgctttc
    35221 tcgaactcaa ccatcgacac aacgcgtttg ttgccacgag cagcgaacag tgccgcttcg
    35281 ttcaccaggt tcgccaggtc agcaccggag aaaccaggag taccacgagc aatgattgcc
    35341 gcgtcgatat cgggtgccaa tggtacgcga cgcatgtgaa ctttcaggat ctgctcacga
    35401 ccgcgaacat ctggcaagcc gaccacaacc tggcggtcga aacggccagg acgcagcagg
    35461 gccgggtcga gaacgtccgg acggttagtc gcggcgataa cgatgatacc ttcgttacct
    35521 tcgaagccat ccatctcaac cagcatctgg ttcagagtct gttcacgttc atcgtgacca
    35581 ccgcccagac cagcgccacg ctggcggcct acggcgtcga tttcatcgat aaagatgatg
    35641 cacggtgccg ctttcttcgc ctgttcgaac atgtcacgaa cacgggatgc acccacaccg
    35701 acgaacattt ctacgaagtc agaaccggag atagtaaaga acggaacttt cgcttcgcct
    35761 gcaatcgctt tcgccagcaa cgttttaccg gtacccggag gaccgaccat caggacgcct
    35821 ttcgggatct taccgccgag tttctggaag cggctcggct cgcgcagata ctctaccagt
    35881 tcagcaactt cttcttttgc ttcgtcgcag cccgcaacgt cagcaaaggt cgttttgatc
    35941 tgatcttccg tcagcatgcg cgctttgctc ttaccaaacg acatggcacc tttgccaccg
    36001 ccgccctgca tttgacgcat gaagaagatc cagacaccaa tcagcaacag catcgggaac
    36061 caggagatga agatagaagc cagcaggctt ggttcttcag gcggttcacc gacaaccttg
    36121 acgttcttgg tcaacaggtt atccagtaat ttcggatcct gaaccggaat gtaagtggta
    36181 taacggttac tatctttctt ggtaacgttg atttcacgtc cgttgatacg cgcttcacga
    36241 acctggtcgt tattgacctc ttgtaggaag gtagagtaat ccaccttacg gccattagac
    36301 tcgctgggcc caaagctctg gaatactgac atcagcacaa cggcaatgac cagccagagt
    36361 attaggtttt tcgccatgtc actcaaggga ttaacctctt attacaactg tgttaaaaac
    36421 agcgtcagga tactctatat ccagcatctt tcaaactttc gtctgaaatc tcccggttag
    36481 ggtttacgcc cggtcgctac aatatacact tctcgcgaac gtgcacgaga agagtccggc
    36541 ttacgaactt tgaccttcgt aaacagggag cgaatttccc ttagatactc atcgaaacct
    36601 tcgccctgga acaccttcac tacaaaactg ccacctggcg ctaatacatc acgacacatt
    36661 tctagcgcca gttccaccag atacatggct cgggggatat ccaccgccgg tgttccgctc
    36721 atgtttggtg ccatatcgga catgacaacc tggactttgc tgtcgccaac gcgctccagc
    36781 agtgctttca tcaccagttc atcacgaaaa tcgccctgaa gaaagtccac accaacgata
    36841 ggatccatag gtaaaagatc gcaagcgatg atgcggcctt tgccgccaat ttgggtgacc
    36901 acatattgtg accaaccacc cggagcagca ccaaggtcga caaccgtcat tcccggttta
    36961 aagagtttgt cactttgctg tatttcatca agtttaaacc aggcacggga acgtaatccc
    37021 tttttctgtg cctgttgaac atatttatcg ctaaagtgtt cctgaagcca gcggctggag
    37081 ctggcagaac gcttcttacc tgtcatttaa ctttcccatg gggataactc atcgtaacca
    37141 attgcgtaaa tttttacgcg cctatttggt gatatatggg agatggcggt agaatgaccc
    37201 gttttcaatc ccaacgtaag caaaaaatac gatgaatctg agtactaaac aaaaacagca
    37261 cctgaaaggt ctggcacatc cgctcaagcc agttgttctg cttggcagta atggtttgac
    37321 cgaaggggtg ctggccgaga ttgaacaagc gttagagcac catgaactca tcaaggtgaa
    37381 aatcgccacc gaagatcgcg aaacaaaaac cttgatcgtg gaagctatcg tgcgcgaaac
    37441 cggcgcctgt aatgtacagg tcatcggtaa aacgctggtg ctttatcgcc caactaaaga
    37501 acgtaaaatc tcgctgccac gctaagatta tcctaaagtt acacacattt gctgtgtaaa
    37561 acgaggggtt ttccgcaggc aggagagcaa aatgccacgc tctgttcgtt gataaaaggc
    37621 cgcagagcgg cctttttcct ttctttacaa tacatcaaca tcttgagtat tgggtaattc
    37681 ttacaggtat tccaccttaa ttacttcaaa ttctacttcg ccgcccggcg ttttgatgac
    37741 cacaacatca tcttcttctt tgccgatcag gccacgagca ataggcgagt ttacagaaat
    37801 caggttttgt ttaaagtcag cttcgtcatc gccaacgatg cgataagtct gttcttcgtc
    37861 agaatccaga ttcagcaccg ttacggtagc accaaaaata acgcgcccat tgttgggcat
    37921 tttggtgaca tcaatcacct gcgcgtttga cagcttggct tcgatgtctt tgatacggcc
    37981 ttcgcagaag ccctgctgtt cacgagctgc gtggtattcg gcgttttctt tcaggtcgcc
    38041 atgctcacgc gcttccgcga tagcagcaat gatttcagga cggcgcacag atttcagaaa
    38101 atccagctct tcgcgtaatt tttcagcgcc gcgtaaggtc atcggaatag cttgcatttg
    38161 ttatacctct tgaatattcc tgatggggca agtcttcacc caccccggct gttcggcctg
    38221 cccggcataa ctcctcaccg ggaccagaag caaaaaaata ccgacccggg tacaagtccc
    38281 aggtcagcta caattcacat tttgatggtc attttaccct gaagttcccg aagggtcatc
    38341 gtttacttta tagggcgttg cgccgtagta tgacggctcg attccaggtt gttagcgcga
    38401 gattatgcga ttttccagat ttatcatcgg attgaccagc tgtatagcgt tcagtgttca
    38461 ggccgcgaat gttgatgagt acgttactca actccccgca ggtgccaacc ttgccctgat
    38521 ggtgcaaaaa gtcggtgcgt cggcccccgc tattgattac cacagtcagc agatggcgct
    38581 gcccgccagt acccagaaag tgattactgc gctggcggcg ttgattcaac tcggccccga
    38641 ttttcgtttc accacgacgc ttgaaaccaa aggcaatgtg gaaaacggcg tacttaaggg
    38701 tgacttagtg gcgcgatttg gtgccgatcc gacgttaaaa cgtcaggata ttcgcaatat
    38761 ggtcgcgact ttgaaaaaat ctggcgtcaa ccaaatcgat ggcaatgtgt tgatagatac
    38821 ctccattttc gccagccacg ataaagcccc cggctggcca tggaatgaca tgacacaatg
    38881 ctttagcgct ccgcctgctg ccgccatagt tgaccgcaac tgtttctccg tctcgctcta
    38941 cagtgcccca aagcctggtg atatggcttt tatacgcgtg gcatcttatt accccgttac
    39001 gatgttcagc caggtacgca ccctcccccg tggttctgcc gaagcgcaat actgcgaact
    39061 ggatgtggtg ccaagcgacc tgaaccgctt tacgctgacg ggatgcctgc cacaacgttc
    39121 tgagccgctc ccgttggctt ttgccgtgca ggatggagcc agctatgccg gtgcaattct
    39181 gaaagatgag ttaaaacagg cgggtatcac ctggagcgga acactgctgc gccagactca
    39241 ggttaacgaa cctggaacgg tagttgccag taaacagtcg gccccgctgc acgatctgct
    39301 taagattatg ctgaaaaagt cggacaacat gatcgccgat acggttttcc gcatgatcgg
    39361 ccatgcgcgc ttcaatgtgc ctggaacatg gcgggctggg tcggacgccg tgcgtcagat
    39421 cctgcgccag caagccggtg tcgatattgg taacaccatt attgccgatg gttcagggct
    39481 ttcgcggcat aacctgattg cccccgccac catgatgcag gtgctgcaat acattgccca
    39541 acacgacaat gaacttaact ttatctccat gctgccgctt gcgggctatg acggctcttt
    39601 gcagtaccgt gcaggtctgc atcaggcggg cgtggatggc aaagtctcag cgaaaaccgg
    39661 ttcgttgcag ggggtatata acctggcggg attcattacc acagcgagcg ggcaacgaat
    39721 ggcatttgta caatatcttt ctggctatgc agtaaaaccc gcggatcagc gtaatcgccg
    39781 tattccgtta gtgcgttttg aaagccgttt gtacaaagat atttatcaga acaattagtc
    39841 aaaaagaaac cccggcgcat ggccggggtt tcagattatt gacaaagtgc gctttgttta
    39901 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat cgtgcaaatt caatatattg
    39961 caatcatctt gtaggcctga taagtgtagc gcatcaggct tatttggcat tggtcattag
    40021 tgattaacgc ttgtaaatga actcaacgcc ttcttcgtcg tcttcgtccc agtcgtcatc
    40081 ccagtcttca tcatcctctt cagcaatctc ttcaagctgc tgacggtgat aatcatccca
    40141 catgaattcg actttctctg gctgtttcgc ttcttcagcc tgcacgaccg ggttttcaat
    40201 gataaaggtc atcacatccc agcagagatc tttcacgccc agtccgctcg ccgcagagat
    40261 cagataatat ttatcttccc agcccagcgc ttcagcgatc gctttcgctt tctcttcggc
    40321 ttctgcctta tccagcagat cgatcttgtt gaagactaac caacgcggtt tcgccgccag
    40381 atcctggctg tatttttcca gctcgctgat aataatacgc gcgttttcaa ccggatcggt
    40441 gccgtcaatc ggatcgatat cgatgaggtg caacaggacg cggcaacgtt ccaggtgctt
    40501 caggaagcga atgcccagac ctgcgccttc cgcagcgcct tcaatcagtc ctggaatatc
    40561 ggcaacaacg aagctctttt cgttgtccat tcgtaccaca cccagacttg gcaccagagt
    40621 ggtaaacgga taatccgcca ctttcggttt agccgccgat accgcacgaa taaaggtcga
    40681 tttacccgcg tttggcatcc ccaacatacc gacgtcagcc agcagcatca gctccagcag
    40741 cagctcacgc ttatcgcccg gcgtaccgtt agttttctgc cgtggtgtac ggttaactga
    40801 cgatttgaaa cgggtattgc ccagaccgtg ccagccgccc ttagcaacca gcagacgctg
    40861 accgtgttta gtcatatcac ccatggtttc accagtaccc tggtcgatta cacgcgtacc
    40921 taccggcact ttaatcgtca cgtctttacc gcgcttaccg gtacaatcgc ggcttgcgcc
    40981 gttctgaccg cgctctgcac ggaaagattt ttcaaaacga taatcgataa gcgtgttcag
    41041 gttctcgtcg gcttccatcc atacgtcacc accatcaccg ccgtcgccgc catccgggcc
    41101 gcctttcgga atatactttt cgcggcggaa gctcacgcaa ccattaccgc catcacctgc
    41161 aacgaccaga atcgatgctt catcaacaaa cttcatttta ttctccgtaa atcattcgcc
    41221 tgcgcggggt tgcgatacca ccgttgtatg cttacgtaat ccgccccaaa tacgatgacc
    41281 aatggcggaa tacatcgcgc ccgcaaccac gacaaacgca ccgagataac ctaaaaggtt
    41341 taacatcggt ctggcgaaga aatcgggcca ggccagtgat aaaagatctg aaaaaaacag
    41401 cgtaaacagt ggggtgagcg tgatgatcgc gctcacctgc gctgcctgcc agcgagccat
    41461 cgcttccgcc aaggcgccat atcctaccaa ggtattcagt ccgcaaaaaa ttaaacatgc
    41521 gagctgccag tggctaagct gcgctatcac tccaggcttt gccagaggga agagcgcgat
    41581 tgtacataaa gtgtacagta aaaacaggat ctgcggtgag gccagccgac gcaataaaac
    41641 cttttgcgcc acgccataac tcacccaaac cgtcgccgca ccgaccccaa agataactcc
    41701 ccaggtgtaa tcggtgagct ttgtaaatat ctcgaccaga ctggtgttaa aaaacatcac
    41761 cagaccgctc aggagcatca atgcccctac aacctgagtg ctgcgcattt tctctttcag
    41821 gataaatacg ctggcaacca tcatgccaac tggcgagagt tgcccaatca cctgcgaagc
    41881 ggtcggactc aggtattgca aggatgagct gaacaggatg aagttcccaa acagcccggc
    41941 ggtcgccact gccaacaaaa tcaaccagcg tggcttacga aacacgcgta atggcggcaa
    42001 cctcttcttc accgcaagaa tggcaccaag gccaatactc gccatcaaga aacggtaaaa
    42061 cacgattgtc ggaggttcca tcacctccag cacctgcttc attgcgattg gcaacgcccc
    42121 ccagcaaatt gctgtggtaa gcgccaaaag aatgccaatg cctgcctgct gcttcatacc
    42181 gtattccctg caaagcgcat ttgcggatta acgtcgcacc atgcgcattt ttaccggtta
    42241 tcgaatgtaa aaagccccgc aacgtgttgc ggggctttca tccgttaccg ggacgcgaaa
    42301 aacttattca gcttcgatgc tgataaattt acggtttttc gggcctttaa cttcgaattt
    42361 cactttaccg tctgctttag caaacagagt gtggtcacga ccgcaaccta cgttagcgcc
    42421 agcgtggaat ttggtaccac gttgacgaac gatgatgcta cccgccagaa cggattcgcc
    42481 accgaaacgc ttaacgccca agcgtttagc ttctgaatcg cgaccgttac gtgtggagcc
    42541 gccagccttt ttatgtgcca tttgaaatct ctcctcaggt cttaggcgct gatgccagta
    42601 attttcacat cagtgaacca ctgacgatgg ccctgctgct tacgatagtg tttacgacga
    42661 cgaaacttaa cgattttaac tttctcgcca cgaccgtgag caacaacttc agctttgatt
    42721 acgccgccat caacgaaagg aacgccgatt ttgacttctt caccgtttgc gatcatcagc
    42781 acttcagcga actcaacagt ttcgccagtt gcgatgtcca gcttttccag gcgaacggtc
    42841 tgaccttcgc ttactcggtg ttgtttacca ccactttgga aaaccgcgta catataaaac
    42901 tccgcttccg cgcacacctt ttcaatgatt cagagtgcgc tataaatatt cacaataggg
    42961 cgcgaatatt acgcaaaacg cacgcctttg acaagtgcta cagtcaatac acgaagaaaa
    43021 aaacacaact tgtacggtaa cgtttatctg tgccattttt tcagtacaat taccctatat
    43081 tcctaaccat aaaccctaag ttgcctttgt tcacagtaag gtaatcgggg cgaaaagccc
    43141 ggcttttgcg atgaatttag aaaaaatcaa tgagttaacc gcgcaagata tggcgggtgt
    43201 taatgcggca atccttgagc agcttaattc cgacgtccaa ctgatcaatc agttaggcta
    43261 ttacatcgtc agcggcggcg gtaaacgtat tcgtccgatg attgctgtac tggctgcacg
    43321 agctgttggc tatgagggaa atgcgcatgt caccattgct gccctgatcg agtttatcca
    43381 cacggcgact ctgctacacg acgacgttgt ggatgaatca gatatgcgca ggggtaaagc
    43441 taccgccaac gccgcatttg gcaatgccgc cagcgtgctg gtaggcgatt ttatttatac
    43501 ccgcgcgttc cagatgatga ccagcctcgg ttcactcaaa gtgctggaag tcatgtcaga
    43561 agctgtaaac gtcatcgcag aaggtgaagt tctgcaactg atgaacgtta acgatccgga
    43621 catcactgaa gaaaactaca tgcgcgttat ctatagcaaa accgcgcgtc tgtttgaggc
    43681 tgccgcgcag tgttccggga ttctggctgg ctgtacgccg gaggaggaga aaggcctgca
    43741 ggattatggg cgctatctcg gcactgcttt ccagttgatc gacgatttac tcgattacaa
    43801 tgccgatggc gaacagttag gtaaaaacgt cggcgacgat ctgaacgaag gtaaaccgac
    43861 gctgccgctg ctgcatgcga tgcatcatgg cacaccagaa caggcacaga tgatccgtac
    43921 cgccatcgaa caaggtaacg gtcgccatct tctggaaccg gttctggaag caatgaacgc
    43981 ttgtggatct cttgaatgga cgcgtcagcg cgccgaggaa gaagcagaca aagccatcgc
    44041 agcgttacag gtgctcccgg acaccccttg gcgagaagca ctcatcggcc tcgcgcacat
    44101 cgctgttcaa cgcgatcgtt aatcccctcc cttcatcccg cgcagtgcgc gggatgattc
    44161 cagtaaattc cataaaaaca ctaattcagc gctataacac ctgaaaaaag ggcgatgaat
    44221 ctttatatat tctgaatatt cacactcttt acaggaactt tttagcgcaa tagaccatca
    44281 ggagtatagt gatgcccgac agaagaagtg ttctgaatga aagcgactaa cttaaggagt
    44341 gaggaaagat ggaaagtaat ttcattgact ggcatcccgc tgacatcatt gcaggtttgc
    44401 gcaagaaagg aacgtcaatg gcggcggaat ctcgcagaaa tggtttgagt tcctcaacgc
    44461 tggcaaacgc attatcgcgc ccatggccga aaggagagat gattattgcg aaagccctgg
    44521 gaactgaccc ctgggttatc tggccatcac gctaccatga tccgcagacc catgagttta
    44581 tcgacagaac gcagttgatg cgtagctaca ctaaaccgaa aaaatgagtg gtctggcggt
    44641 agccccgcga acggggctgc cagctctcag acgattactc gcctttcaca cgctcaatat
    44701 ttgcacctaa agcgcgcagt ttgtcttcaa tgcgttcgta gccacgatcg atgtgataaa
    44761 tacgatcaac caccgtcgtc ccttccgcaa tacagccagc cagcaccagg cttgctgatg
    44821 cacgcagatc ggttgccata acctgtgcac cagaaagttt ttcaacacca tgacaaataa
    44881 cggtattgct ttcgatttcg gcgtgcgcgc ccatacggct cagctctggc acatgcataa
    44941 agcggttttc aaagaccgtt tcggtgatga acccggtccc ttcagccacc aggttcaaca
    45001 gcgtgaactg ggcctgcata tcggtcggga atgccggatg cggcgcggta cgtacgttaa
    45061 cagccttcgg acgtttgcca tgcatatcca ggctaatcca gtcttcgccg acttcgatgt
    45121 ccgctccagc atcacgcagt ttcgccagca cggcgtccag agtatctggc tgcgcgttac
    45181 ggcagataat tttgccacga gagatcgccg ccgccaccag gaaagtaccg gtttcgatac
    45241 gatccggcag cacgcgatag acaccgccgc ctaaacgttc cacaccttcg atgacgatac
    45301 gatcggtgcc ctgaccgcta attttcgcac ccagcgtaat caggaagttc gcggtatcga
    45361 cgatttccgg ttcacgcgct gcgttttcaa taatcgtggt gccttccgcc agggttgcag
    45421 cacacatgat ggtcaccgtt gcgccaacgc tgactttatc catcacgata tgtgcgcctt
    45481 tcagacgacc atcgacggaa gctttaacgt aaccttcttc cagtttgatg gtcgcgccta
    45541 attgttcgag gccagaaatg tgtagatcaa ccggacgcgc accgatcgta caaccgccag
    45601 gtagtgaaac ttgcccctga ccaaagcgcg ctaccagcgg ccccagcgcc cagatagaag
    45661 cacgcatggt tttaaccaga tcgtaaggtg cgcagaatac attaacgtcg cgggcatcaa
    45721 tatgcacaga accattacgt tctactttcg cacccagctg gcttagcagt ttcattgatg
    45781 tatcgacgtc tttcagtttc gggacgttct ggatctctac cggttcttcc gccagtagtg
    45841 cggcaaaaag aataggcaga gcagcatttt tagcgccgga aattgtgact tcgccctgga
    45901 gcttcgttgg cccctgaaca cgaaatttat ccatttagtt tgttctcagt taacaattca
    45961 tatccgctac cggcgaatcg cccatagctc aaaagccgtt cagtttgcga tcgcgcgccc
    46021 actccgcagg ggtatacgct ttgatcgaca cagcatgaat gcggttatcc gcaatatatt
    46081 ccatcagcgg gccatagacc gtctgctgtt ttttaacccg actcataccg tcaaacaact
    46141 cacccacggc aataacctga aagtggctgc catcgccgga aacgtggact tcctggaggg
    46201 agagagcgtt catcaacacg ctctgaattt cattattttc catgggatct tcaatcatca
    46261 gttaataaac cagcgaaaca tcttagagca aagttgcgct ggcataaata agcaaaaagc
    46321 ctcgctgata aatcagacaa ggctcgactt gcaggcaggt ttgccggaca ggcggttaac
    46381 gccatatccg gcctgaaaaa atttaacgag gcagaacatc agcaggcaaa ttatacaatt
    46441 tcgcgagggt atacactttg tcgtttaccc cctgaagcgt cacgttgttg ccctgctttt
    46501 tcgccagatc gataagatgg agcagcagtg ccagtccccc cgtatccacg cgggacacac
    46561 ggctaagatc gatgcaggta atccccttca ccgcctcctc acgcatttcc caaagcggta
    46621 gcagaacgtc ctgatccagc tctccggata acgccagcgt gtcacccgtc tgcatccagc
    46681 tcagtgactc gctcattatt ttttctcttc cagagtgatt ttctgttgag aaatcgattt
    46741 cagttgcgca gtcaggccgt cgataccttt ggtacgcagc agcgttcccc actcgttttg
    46801 tttggtggtg atcatactga cgccttcagc aatcatgtcg taagcttgcc agtttcccgt
    46861 ctgggagttt ttacgccact ggaagtccag acgcaccggc ggacggccat tcgggtcaat
    46921 aatggtaacg cgaataggca caatggtttt atcgcccagc ggctgttctg gcgcaatctg
    46981 ataggtttga ccgtgataca tcgccagcgc ctgaccgtaa gcctgcttca ggtactcacg
    47041 gaaagcggca aagtaggctt cacgttgagc aggggtcgca ctcttgtaat actggcccag
    47101 caccagcgca ccggcgtatt tcacctgtac gtatggcagc agttcctgat caacaatggt
    47161 acgcagataa tccgggttgg cccgaatttg cggttgctca ttcttcaggc gatcgaacgt
    47221 tttctgcgcc gcctcgtcca tcagcttata aggattggtc tggtctgccg cggttgccgc
    47281 actcagaggt gcaatcacca gcaaagcgac catcattaaa cgtttaaaca tgcgtcggtt
    47341 ctcctgaaat tatttcgttg tacccacagg ttcagtggtt tcattattac ctggcgcagc
    47401 agctggcgca tcgccactat tcttattgtc atcgccttta ctaccgtaaa ggaactgacc
    47461 aatgagatct tccagcacca tcgcagactt ggtgtcctga attgtatcgc catccttcag
    47521 gatagcagtc cccagttccg ggtcttcaaa accgacgttt aatgccagat attgttctcc
    47581 cagcaggccg gaagtacgaa tgctcagcga actggtatca ggaatgtggt tataacgttg
    47641 ttcaatttcc agcgttacgc gcggcagata ggttttcggg tccagcgtaa tatccgccac
    47701 ccggcccaca acaacaccac caatgctgac cggagagcgc gctttcaggc cgccaatgtt
    47761 atcgaacgtc gcataaagcg tgtaggtcga ttcagtacgt atggacgtca cgttcgccgc
    47821 cttcaggcaa acaaacagcg ccgccagcag tgctgctaat aaaaagatac ccacccaaat
    47881 ttcatttttt ttcgtttgca tgaactcaat tcccaaacat caatgcggtc agcacaaaat
    47941 ccagccccag aacagccaga gacgagtgga caacggtgcg agtggttgcc cggctaatcc
    48001 cggcagacgt cgggatggcg tcgtagccgt taaacaacga aatccacgtc accgtgatgg
    48061 cgaacaccac gctcttaatc agacagttga ccagatccat acgccagtcg acggcatttt
    48121 gcattgccga ccagaagaac ccgctatcaa tgcctttcca actgacgccg accagcgatc
    48181 cgccccagat ccccacggcg acgaagataa ccgtcaacag tggtaatgaa ataaccccag
    48241 cccagaaacg gggagaaata acccgacgca gcggatccac cgccatcatc tccatactgg
    48301 agagttgctc tgtagcgcgc atcaggccga tttctgcggt taacgccgaa ccagcacgcc
    48361 cggcaaacaa cagcgcggca accaccggcc ccagttcacg cagtagcgat aacgccacca
    48421 gcatacccag actggtttcc gcgctataag tggtcagaac cagataacct tgcagcccca
    48481 acaccattcc gatgaacacg ccagaaacta caataatcag catcgacagg acgccgacat
    48541 tatagagctg gcgcaccagc agcggcgcat gtttgcgaaa ttccggtttg ccgaccagcg
    48601 cattgaataa cattaacccg gcccgcccga acgttctcag ggttttaatc cctttatgtc
    48661 cgagcgacgc cagcgcattt aacagcatga gtggcttaac tccctggtaa aagatcagcg
    48721 tgataatcgc cggcaggata gcggaacgga acaggcccgt cagctatccc gtccagaaac
    48781 tgacgtacgc gcggatcagg attcgtctgc aacgcctggg cactgccatg agcgacaatt
    48841 tttttgtccg ccaggatcca ggcgtgatcc gcaatactta acacttccgg cacatcgtga
    48901 gaaaccacca cacaagtcac gcccagcgcg ctgttcagct cagaaatcag cttcaccagt
    48961 acgcccatgg tgatgggatc ttgcccaaca aaaggttcat caaacatgat gagatccggc
    49021 tccagcgcaa tcgcacgcgc cagcgctgca cgccgcgcca tcccaccgga aagttcagaa
    49081 ggcattagtt tagccgctcc acgcagcccc acggcctcca gcttcatcat caccgtacta
    49141 tgcaacaatg gcgcgggaag ttgggtatgt tcgcgcagtg gataggcgac gttgtcaaat
    49201 acgttcatat cagtgaacaa cgccccggac tgaaataaca tgctcatccg tttacgcact
    49261 gtatacaggc gcgaacgaga catcgccgga atattctcac catcgaaaag gatctcacca
    49321 tgatctggtg cgatttgccc gccaatcaga cggagtagcg tcgttttacc gatgcccgat
    49381 ggccccatga tcgccgtgat cttccctcgc ggcacggtca gggaaatatt atcgaagatg
    49441 cagcgattgc cacgcgtaaa actgacatcg cgcatatcga ctaaattcgc cacagactgc
    49501 tccataattc acccttcgtc ttgcgttgat tttctaagca tggcgctcaa tttaaccttg
    49561 aacccaacat atttacagaa tattacccgc cgtggttagc gaaagctggc atttgtttta
    49621 ctttttagcc gcataaagtc aaaattaaga atccgttacg gctttctgaa aatcttcagc
    49681 ggaccggcga gtatacctga agaaaggacg ttagatgctt ttagctacgg cactgttaat
    49741 tgttggttta cttttggtcg tttacagtgc cgaccgcctg gtttttgccg cgtctattct
    49801 ttgccgaacc tttggcatcc cgccgctgat catcggcatg acggtggtca gtattggtac
    49861 atcgttacca gaaatcatcg tctcgcttgc cgcgtctctg cacgaacaac gcgatttagc
    49921 cgttggtaca gccctcggct caaacattat caatatattg ctgatcctcg gtctggctgc
    49981 gctggttcgt ccttttaccg tccattccga tgttctgcgc cgtgaattac ccttaatgtt
    50041 gttggtcagc gtggtggccg gttccgtact ctatgacgga caacttagtc gaagcgatgg
    50101 tatctttctc ctctttctgg ctgtgctatg gctgctgttc attgttaaac ttgcacgtca
    50161 ggggactgac agcctgacca gagagcagct tgcagagctg ccgcgtgacg gcggattgcc
    50221 cgtcgcgttt ttatggctcg gcattgcgct tatcatcatg ccagtggcca cgcggatggt
    50281 ggttgataac gccacggtgc tggcgaatta ctttgccatc agcgagttga cgatggctct
    50341 gacggcaatt gctatcggaa ccagtctgcc ggaactggca accgcaatag cgggggttcg
    50401 caaaggtgaa aacgacattg ctgtcggaaa tatcattggc gcaaacattt ttaatattgt
    50461 catcgtgttg ggtttacccg cgctgataac gccaggagag attgatccac tggcgtacag
    50521 tcgtgactac agcgtgatgt tgctggtgag cattattttt gcgttgctgt gctggcggcg
    50581 ctccccgcaa ccgggccgtg gtgtaggggt attattaact ggcggattta tcgtatggct
    50641 ggcgatgttg tactggttat cgccaatact cgttgaataa ctggaaacgc attatgtcgc
    50701 acgtagagtt acaaccgggt tttgactttc agcaagcagg taaagaagtc ctggcgattg
    50761 aacgtgaatg cctggcggag cttgatcaat acatcaatca gaatttcacg cttgcctgtg
    50821 aaaagatgtt ctggtgtaaa gggaaagttg tcgtcatggg gatggggaaa tcggggcaca
    50881 tcgggcgcaa aatggccgca acgtttgcca gcaccggtac accttcattt ttcgtccatc
    50941 ctagtgaagc cgcgcatggt gatttaggca tggtcacccc acaggatgtg gtgattgcta
    51001 tctctaactc aggtgaatcc agcgaaatca cggccttaat tccagtgctt aagcgtcttc
    51061 acgtaccgtt aatctgcatc accggtcgcc cggagagcag catggcgcgc gccgcagatg
    51121 tgcatctgtg tgttaaagta gcgaaagaag cctgtccgtt agggctggca ccgaccagca
    51181 gcaccaccgc cacgctggtt atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg
    51241 gctttactgc tgaagatttt gcgctctcac acccaggcgg cgcactgggt cgtaaacttc
    51301 tgctgcgcgt aaacgatatt atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg
    51361 ccagtctgcg tgacgcattg ctggaagtta cccgcaaaaa tcttggtatg actgtcattt
    51421 gcgatgacaa tatgatgatt gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg
    51481 atatgggcgt ggatgttcgt cagttaagta ttgccgatgt gatgacgccg gggggaatac
    51541 gtgtgcgccc tggcattctg gccgttgagg cactgaactt aatgcagtcc cgccatatca
    51601 cctccgtgat ggttgccgat ggcgaccatt tactcggtgt gttacatatg catgatttac
    51661 tgcgtgcagg cgtagtgtaa agattcaagg ataaacaaca atgagcaaag caggtgcgtc
    51721 gcttgcgacc tgttacggcc ctgtcagcgc cgacgttatg gcaaaagcag agaacattcg
    51781 tctgctgatc ctcgacgttg atggcgtact gtcagatggc ctgatttata tgggcaataa
    51841 cggcgaagag ctgaaagcgt tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac
    51901 ctctgatatt gaagtcgcta tcattaccgg gcgaaaggct aaactggtag aagatcgttg
    51961 tgccacattg gggatcactc acttgtatca ggggcagtca aacaaactga tcgcctttag
    52021 cgatttgctg gaaaaattgg cgattgcccc ggaaaatgtg gcttatgttg gcgatgatct
    52081 catcgactgg ccggtaatgg aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc
    52141 actgttgatc ccgcgcgccg attacgtgac gcgcattgct ggcggtcgtg gcgcagtgcg
    52201 cgaagtttgc gacttattac tcctggcgca gggcaaactg gatgaagcca aagggcaatc
    52261 gatatgagta aagccagacg ttgggttatc attgtgctat cactggcggt tctggtgatg
    52321 atcggcatta atatggccga aaaagacgat accgcccagg tggtcgtcaa caacaatgat
    52381 cccacctata aaagcgagca tacggacacg ctcgtctata acccagaagg ggcactaagc
    52441 tatcgattga ttgctcaaca cgttgaatat tattccgatc aggccgtttc gtggtttacg
    52501 cagccggtac ttaccacgtt tgataaggat aaaatcccga catggtccgt aaaagcagat
    52561 aaagccaagc tgaccaatga ccggatgctc tatttatatg gacacgttga agtcaacgca
    52621 cttgtgccag actctcaact tcgcagaatc acgacggata acgcgcagat caatctggtg
    52681 acgcaggatg ttacctctga agacctcgtc acgttatacg gaacaacatt taactccagc
    52741 ggtctgaaaa tgcgcggcaa cttacgcagc aagaacgccg agctgattga aaaggttaga
    52801 acatcctatg aaattcaaaa caaacaaact cagccttaat cttgtgcttg ccagctcact
    52861 tctggccgcc agcattccgg catttgccgt aaccggagac actgatcagc cgatccacat
    52921 tgaatcggac cagcaatctc ttgatatgca aggcaacgtg gttaccttta ccggtaatgt
    52981 catcgtcacc cagggcacca tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg
    53041 cggcgaacaa ggtaaagaag tgattgacgg atacggtaaa ccggcaacgt tctaccagat
    53101 gcaggacaac ggtaaacccg ttgaaggtca cgcttcccag atgcactacg aactggcaaa
    53161 agattttgtc gttctgacgg gtaatgctta tctgcagcag gtcgatagca acattaaggg
    53221 cgataagatc acttacctgg tgaaagagca gaaaatgcag gctttcagcg acaaaggcaa
    53281 gcgcgtaacc accgttctgg tgccgtcgca gctgcaggac aaaaacaaca aaggccagac
    53341 cccggcacag aagaagggta attaattcgt tatggcaaca ttaactgcaa agaaccttgc
    53401 aaaagcctat aaaggccgtc gcgtggtaga agacgtcagc ctgaccgtca actccgggga
    53461 aattgtcggt ctgctggggc caaacggtgc cggtaagacc accactttct acatggttgt
    53521 aggcattgtg ccgcgcgatg cgggcaacat cattattgat gatgacgata tcagtctgct
    53581 gcctctgcat gcacgcgcgc gccgcggtat cggctatctg ccacaggaag cctccatttt
    53641 ccgtcgcctc agcgtttacg ataacctgat ggcggtactg caaattcgtg acgacttgtc
    53701 tgctgaacaa cgtgaagacc gcgcgaacga gctgatggaa gagtttcaca ttgagcacct
    53761 gcgtgacagc atggggcagt cactctccgg gggtgaacgt cgccgtgtag aaattgcccg
    53821 cgcactggct gcgaatccga aatttattct gctcgacgaa ccgtttgccg gggttgaccc
    53881 gatctcggtt atcgacatta aacgcatcat tgaacacctg cgcgacagcg gcctgggcgt
    53941 gctgatcact gaccacaacg tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt
    54001 cagtcagggg catttgatcg cccacggcac gcctacagaa atcttacaag acgaacacgt
    54061 taagcgtgta taccttgggg aagacttcag actctgatag ggtagaagtt tgcgacgttt
    54121 tagcaggaga gtacgattct gaacatgaag caaggtttgc aactcaggct tagccaacaa
    54181 ctggcgatga cgccacagct ccaacaggca attcgtctgt tgcagttgtc gacgctggaa
    54241 cttcagcagg agctacagca ggcgctggag agtaatccgc tgcttgagca aatcgacact
    54301 catgaagaaa ccgacacccg cgaaacgcaa gacagtgaaa cgctggacac cgccgacgcg
    54361 ctcgaacaaa aagagatgcc ggaagagctg ccgctcgatg ccagttggga caccatttac
    54421 accgctggta caccatccgg caccagcggt gactacattg acgacgagct gccggtctac
    54481 cagggcgaaa cgacgcagac cttgcaggat tacctgatgt ggcaggtcga gctgacaccg
    54541 ttttccgaca ctgaccgcgc tattgctacc tctatcgtcg atgccgttga tgaaaccggt
    54601 tatctgactg tcccgctgga agatattctc gaaagtatgg gcgatgaaga gatcgacatc
    54661 gacgaggttg aagccgtcct taagcggatc caacggtttg acccggtcgg tgtagcggca
    54721 aaagatctgc gtgactgcct gctaatccaa ctctcccaat tcgataagac cacgccgtgg
    54781 ctggaagagg ccagattgat cattagcgat catctcgatc tgttagccaa tcacgacttc
    54841 cgcactttaa tgcgcgtcac gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg
    54901 atccagtcgc tcgatccgcg ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc
    54961 attccagatg tgctggtgcg taagcataac ggtcactgga cggtagaact caacagtgac
    55021 agcattccgc gtctgcaaat caaccagcac tacgcctcga tgtgcaataa cgcgcgcaac
    55081 gatggtgaca gccagtttat ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt
    55141 ctggaaagcc gtaacgatac gctactgcgc gtgagtcgct gtatcgttga acagcagcaa
    55201 gccttctttg agcaaggtga agaatatatg aaaccgatgg tactggccga tatcgcccag
    55261 gctgtcgaaa tgcatgaatc gacgatatct cgcgtgacca cgcaaaaata cctgcatagt
    55321 ccacgaggca tttttgaact gaagtatttc ttttccagtc acgtcaatac cgagggcggc
    55381 ggcgaagctt cctccacggc gattcgtgca ctggtgaaga aattaatcgc ggcggaaaac
    55441 ccagcgaaac cgttgagcga cagcaagtta acctctttgc tgtcggaaca aggtatcatg
    55501 gtggcacgcc gcactgttgc gaagtaccga gagtctttat ccattccgcc gtcaaaccag
    55561 cgtaaacagc tcgtttgacc caaccgataa ggaagacact atgcagctca acattaccgg
    55621 aaataacgtc gagatcaccg aggcactgcg cgaatttgtt acagctaaat ttgccaaact
    55681 tgagcaatat tttgaccgaa tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac
    55741 ccacacctca gatgcaacac tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg
    55801 tcaggacatg tacgctgcca ttgatggctt aattgataag ctggcacgcc agttgaccaa
    55861 acataaagat aaactgaaac aacactaatt gtccgggcaa ttagcatgtg catggcggcc
    55921 tgttgtgcgg cacaacgggc catttgtacg gttaatgctc cgagcctgtt ccactgtttg
    55981 agtgggcagg ttcttaggtg aaattatgac aaataatgat acaactctac agcttagcag
    56041 tgttcttaac agggaatgta cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct
    56101 ggaaatcatc agcgagctgg cggcgaaaca acttagcctg ccgcctcagg tggtttttga
    56161 agctatcctg acgcgggaaa aaatgggcag taccggtatc ggcaatggta ttgccattcc
    56221 gcatggcaaa cttgaagaag atactctgcg tgccgttggc gttttcgttc agctcgaaac
    56281 gcctatagct ttcgatgcca tcgacaacca accggtggac ttactttttg ccctgctggt
    56341 gccggcagac caaactaaaa cgcacctgca tactctgtcg ctggtggcga aacgtctggc
    56401 ggacaaaacc atctgccgcc gtttacgcgc agcccagagc gatgaagagc tgtatcaaat
    56461 cattacggat accgaaggta ctccggatga agcgtagtta ttcggtaatg tctcttttag
    56521 acgttgtgag gagaaacagt acatggtact gatgatcgtc agcggacgtt caggttcagg
    56581 taaatctgtc gccctgcgtg cgctggaaga tatgggtttt tactgcgtgg ataaccttcc
    56641 cgtagtgttg ttacccgatc tggctcgaac tctggccgat cgcgagattt ctgccgccgt
    56701 cagcattgat gttcgtaata tgccggagtc accagaaata ttcgaacagg cgatgagtaa
    56761 cctgcctgac gctttctcac cgcaactact gttcctggat gccgaccgta ataccttaat
    56821 tcgtcgttac agtgacacgc gccgactgca tccgctttcc agcaaaaacc tgtcgctgga
    56881 aagtgctatc gacaaagaaa gcgatttgct ggagcctctg cgttcgcgag cggatctgat
    56941 tgtcgatacc tcagaaatgt ccgttcacga gctggctgaa atgctgcgta cccgtctgct
    57001 gggtaaacgt gaacgcgaac tgaccatggt ctttgagtct ttcggcttca aacacggtat
    57061 ccctatcgat gcagattacg tctttgacgt gcgcttcttg ccgaacccgc actgggatcc
    57121 gaaactgcgt ccaatgacag gtcttgataa acctgtcgcc gcgttcctcg accgccacac
    57181 agaagtacac aattttatct accagacgcg aagctatctt gagctatggt tacctatgct
    57241 ggaaaccaac aaccgtagct acctgacggt cgccattggt tgtaccggcg ggaagcaccg
    57301 ttcggtgtat attgcagagc aactggcaga ctacttccgc tcgcgcggta aaaacgtcca
    57361 gtcacgccat cgtacgctgg aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc
    57421 acaaacaagc tgggcatgca tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt
    57481 tttgacgctg aagtgctgtt acgtaatgac gaaggcaccg aggctgaagc caacagcgtt
    57541 attgcgctgc tgatgttgga ttctgccaaa ggacggcaga ttgaagttga agcgaccggt
    57601 ccacaggaag aggaagcact ggccgccgtt atcgccctct ttaattctgg ttttgatgaa
    57661 gattaatctt catcactttg acatacaact cccttcaagc tcccccgaca ataagaaaat
    57721 cacgtactga aatcgttctc aatcaacgtc atttgtacat tttgtgcgct tttcactttc
    57781 agaagaacct taagaaaacc ttaagaggca ttgtttaggt tttgtttaag ttaatcgacc
    57841 atactggaga tcgtcagaaa atatttccag gagatggcat gattcgctta tctgaacaaa
    57901 gtcccctggg caccggaaga catcggaaat gttatgcgca tccggaagat gcccaacgct
    57961 gtatcaagat tgtctaccat cgtggcgatg gtggcgataa agagatccgc cgtgagttaa
    58021 agtactacgc gcatcttggt cgccggttaa aagactggag tggaataccg cgctatcacg
    58081 gtaccgttga gacggattgc ggaaccggtt atgtctacga tgtgattgcc gattttgacg
    58141 gcaagccttc cattacgctg accgaatttg ctgaacaatg ccgttacgaa gaagacattg
    58201 cacaactgcg ccagctactg aaacagctga agcgttattt gcaggataac cgtatcgtga
    58261 cgatgtcgct gaagccgcag aatatcctct gtcaccgcat cagtgagtca gaggttatcc
    58321 cggtcgtctg cgacaacatt ggtgaaagca cattgattcc actggcgacc tggtcgaagt
    58381 ggtgttgtct gcgtaagcaa gagcggttat ggaaacgatt tatcgcccaa ccggcgctgg
    58441 ctattgccct gcagaaagat ctgcaacctc gtgagagcaa aacgctggcc ctcacttcgc
    58501 gcgaagctta atccagctgg tgttgttgca taaacggctc accgcctaac tgatacatct
    58561 gccgtaaaat ccacgcctga cggctacgca cgtagcccga tggcgaggag actttgaaac
    58621 gaagtggatt aggtaataca gctgccagta atgcagcttc cgaccgggta agtttgctcg
    58681 cgggtttgtg gaaataacgt tgtgccgcag cttcgacgcc aaacacaccg tcgccaaatt
    58741 cggcgatatt caggtaaacc gtcaggatac gctttttgct ccagactgtt tctatcccca
    58801 gcgttaatcc agcttccagc ccttttcgta cccagctacg cccatcccat aaaaagagat
    58861 tttggccgtc tgctgagaaa tcgttgaagc accgcgaata cggttttcat tgcgctcgtt
    58921 gtgcgccagg gctttctcaa tggaagcgac atcaaagccc cagtgctcag gaaatttctg
    58981 atcttctgcg gcaatcaccg ccagtcccat ccacggcgag atttgatcca tactgaccca
    59041 gtcagaatgt gccacgtaac gaaaattgcc atgcagccag gcgctgacct gtcgctcgac
    59101 cattaccgct gagaagggaa caggcgcaac gctaaacaac gcgatgcccc cgccccagaa
    59161 aacggcgagg acaaccatta accgcaaaag aaaacggcga acgaaactaa acaccgttaa
    59221 gcggctttta ctcattcagc cagaaccagc acgcgggaaa ccagcttatc aatgccgctc
    59281 gccgcttctg caatgttctg cgccagcata tatgctgggg tggtgacaat cttattgtct
    59341 tcatcaacca cgatatcatc gacaggacac ggcacatgct ccgcgcccat ctcttccagc
    59401 acttctgcgg tatcgatatc agtaccgatg gtcaaacgca gcgggaaatc gaaaattttc
    59461 ggcagcatcg ccggggcaat acacataaaa ccaagcggtt ttccggcctg atgcatcgct
    59521 tgtgccagcg cctttaattc acggtcaacg gtgcattcgc taccaagact ggcaaaattg
    59581 cttaaattct tcgccgcgcc aaaccccccc ggcacaatca acgcatccag ttcagcggca
    59641 tcggcctggg ccagaggacg gatttcaccg cgcgttattc gtgccgcttc aatcagcaca
    59701 ttgcgcgttt ccgtcatcgc ttcgccagtt aaatggttga taacatcaac ctgctgctta
    59761 tccggtgcaa agcagaccgc ctgcgcaccg ctgcgtgaaa tagctaacaa cgtcaacacc
    59821 gcttcatgaa tttcagaacc gtcatagacg ccgcatccgc tcagaattac gccaattttc
    59881 ttcattgtga tcatcctttc gcaattgact gaaacacatt aattttttta ataaaaatgg
    59941 tacgcatcac acatttaact gattcatgta acaaatcatt taagttttgc tatcttaact
    60001 gcgtgcggcc tgaaaaacag tgctgtgccc ttgtaactca tcataataat ttacggcgca
    60061 gccaagattt ccctggtgtt ggcgcagtat tcgcgcaccc cggtctagcc ggggtcattt
    60121 tttagtggct tttgccaccc acgctttcag cacttctacg tcgtgacgcc actcttcttt
    60181 catctcttca atccattcac cgacgttatc ttcccaggcg ggaaggtcag gagactgaat
    60241 ttgctgaccc agctgttgca gatggcgtaa ccccactgac cccgccgcac ctttaatttt
    60301 atgtccttcc tcaacaatgc cttttttatc ctgcgccgtc agattcgact ccagcacgct
    60361 gacatagccc ggcatcatct tctcaaacac cgctaacccg tcggtgatca gcttcggtcc
    60421 tacaagttcg agatactgtt ccagcatggg aatatcgagc aatgcttctg atttactgtt
    60481 ctcttctgtc gtcaccgtac tctcctcatc atcctgggta tcccagaatt tcttgatcat
    60541 cgcggttagc gccggaaccg aaagcggctt actcagcaca tcatccattc cagcattgag
    60601 gtactcttgt ttgtctttca gcacgttagc ggttaaggcc accagcggcg gtaaatcctc
    60661 gcgcggataa cgcttcgtca gttcacgaga gatatccagc ccggtcatat ctggcaactg
    60721 aatatccagc aacaccaggt cgtattcgcc cggtttaaac atctccagcg ccgccttgcc
    60781 ggtcatggcg acatcaacgc tgttacctaa tttttccagc acagaacgcg cgacaatcac
    60841 gttcagttca atgtcttcca ccagcagcac attcagcgcc ggtaaaggca tatcgtcttc
    60901 atcaaacgca tcatcgacct cttctgccac cgacggtgcg tggatcgtca acgtaaaggt
    60961 tgaacctttg ccctgttcac tggtaaccgt aatatcgccg cccatatttt tcgccagacg
    61021 acgagaaacg gccagaccaa taccggtgcc ggtggcaggt ttaccgccat gactgtcttt
    61081 cacctggtaa tacatggcga agattttatc cagctcatcc tgcggaatgc cgataccaga
    61141 atcttccact tcaaaatgca gcatatcgcc ttcatcgtag cgcacgcgca cggtaacctg
    61201 gccttgctgg gtgaatttga cggcgttact gatgaggttc cacaggatct gccgtaaacg
    61261 cgtcccgtcg gtaatgacct gatgcggtaa tggcagcgta ggctccaggt taaagcgcaa
    61321 tcctttttgt tgcgcctgca atgcggagag attttccaga tcggcaagga agctggtgaa
    61381 atcaaccggc tgattatcaa gctggacctt gcgccgttcc atcttatcca tgtcgataat
    61441 atcgttaaag atattcccca gcgtgacggc cgaaacatgg atagttttga gatatttttc
    61501 ctgctcggcg gtgagttcgg tatccagcag aatgcggctc aggccgacga taccgttcag
    61561 cggtgtacgc aattcgtgac tgatggtgga aataaacgtc gttttgtcgc ggctggcgcg
    61621 ttcaagcgca tcctgatacc gcttacgctc ggtaatgtcg cgaccaaagc ccatcaaacc
    61681 gtgacgttta cccacgcggt cgtagtacgg cactttacgg atttcaaagc aggctttgcg
    61741 cccgtccggg taatccagcc actgttcata ggtcagtgac acattatgac ggaacacttt
    61801 ttcatcggtt tcaatgactt ttgcggcggc ttccggtgag taaacatcag caggtttcag
    61861 gtgaaccagt tgtttttcgc tttttccggt cagcagctcc atcgcgcggt tacagccgga
    61921 aaactcttta tcttcgttac gataaaaaac caagtcgggt gaagcatcaa ggaaggaacg
    61981 taagaatgag gattgctgct cgagctgaat ttgtgtctct tcgcgctctt tgatttcaat
    62041 tttcagttgg ccgaaggttt cctgcagttc tgcttccgct ttttcacgaa cggcaatttc
    62101 ctgatttagc tgggcaatat tatcttttaa ctgaacgttg aggctcaaat cgcgctcgcg
    62161 catctcctcc agtttttgca ccagccgtga cagacgttgt cgtgactcct ccagttgctc
    62221 gacgaccacc gatagaaagt agaccgccca cggcgtaatc agcaaaccaa agaagataga
    62281 acgaataaca tcaatgcttt cgacctgacc atgcagcacc atggttaccg ccatttgtac
    62341 cacaatggca agaacgacga gggccagcgc cagcaacatt gagaagcgca ccagacctaa
    62401 cttcatcatc aggtcaacat aatactgcgc cagcagacga atttgcttca ttagggaatt
    62461 ccttcacgac aacctgcccc aataataccc aattctgaca gttacgttat aaattgtgtg
    62521 agaaatgcgg aattcccctc aacgacctac tccgttggag gtagccacgg acgtcccagc
    62581 gccgatccct gtacgccatg ttcattcaga tagcgatcca gctcgaccat tcccgtccat
    62641 cgattttcgc accacagcgg agcaagcagc gtcgggcgac gggcgctggc ggaaatgcgg
    62701 tggtagatca cttccggtgg cgtatggcga atcatttctc cggcagtgag cgtataatcc
    62761 tccagttcaa taccgttcaa acgccccgct tcccaggctt tcgccataat gctgcctttc
    62821 acaatatgca gcggatgcag ctttatgccg tccacgctgg tttcaaccac tcgctccagc
    62881 gtttgcaaac attcagcctg cccttcgcca ggcaggccga caattaagtg ggaacacacc
    62941 ttcagcccgc gctgacgtgc cagctgggtt gtacgctgat aacaggcaaa atcatggccg
    63001 cggttgatgc gatgcagtgt tttgtcgtgg gcggtttgta gccccagctc cagccacact
    63061 tcgtaaccct ggtccttata ttcgcaaagc agatccagca ccgcatccgg cacgcagtcc
    63121 gggcgggtac caacacacaa accgacaata ttggcctggc tcaccgcctg ctgatacata
    63181 gaacgcagca cctgaacttc cgcaaaggtg ctggtatacg cctgaaagta ggccagatag
    63241 cgtttagcgc ggttaactaa attcgcctgg tgcgccagtt gctcggcaat ggaacgatgc
    63301 tgctgcgctt catcggcaaa cgaggcaaca ttacagaatg tgcagccacc acgcccgatg
    63361 gtaccgtcac ggttagggca gctaaaaccg ccgtgcagcg tcagcttatg caccttttgc
    63421 ccataacgac gggtgagatc accaccaaac atattgacta atttctgtaa ctgcataatc
    63481 tgatagaccg cgccttgaaa agaggccaaa gcctgccatt tttagctcaa ttcggcgatg
    63541 acctggatca atcgtcccgg cctgctttta tcaactgcat aatcaatcaa aattaccgaa
    63601 atttcatgca caatcacata aatcactttt gcttatcttg tgtcagattt ttttatctcc
    63661 ttatggattt taggcaaaaa cagtagcatg aaacgtcatt accaattaag gcagtataaa
    63721 atgctggttt tctcgtcagt tcaaggcagg ataagggtta acacgccttt atgacagtca
    63781 ggaattgact gtttctctaa cgactctcct tttagcctta aagataaaat ctattttaat
    63841 ttcagctatt taataaagaa ttttgcgcta aagcacattt ctgtaccaat aagcttgcca
    63901 tttgacctgt atcagctttc ccgataagtt ggaaatccgc tggaagcttt ctggatgagc
    63961 agcctgctca tcatatttat gcagtaattg agatcccctc ttcaccgtat taaccgatgc
    64021 gaaaaggaca acaagggggc gaatgcgagg cgcgcgtatg acacgcaaac cccgtcgcca
    64081 cgctctttct gtgcccgtgc gcagcggttc ggaagtgggg ttcccgcaga gcctggggga
    64141 ggttcacgat atgttgtacg ataaatccct tgagagggat aactgtggtt tcggcctgat
    64201 cgcccacata gaaggcgaac ctagccacaa ggtagtgcgt actgcaatac acgcactggc
    64261 ccgcatgcag caccgtggcg cgattctcgc cgatggtaaa accggcgacg gttgcggctt
    64321 gctgttacaa aaaccggatc gcttttttcg catcgttgcg caggagcgcg gctggcgttt
    64381 agcaaaaaac tacgctgtcg ggatgctctt cctgaataaa gatcctgaac tcgccgctgc
    64441 cgcacgccgc atcgttgaag aagaactgca acgcgaaacc ttgtcgattg tgggctggcg
    64501 tgatgtcccc actaacgaag gcgtgctggg tgaaatcgcc ctctcctctc tgccacgcat
    64561 tgagcaaatt tttgtgaacg ccccggcagg ctggcgtcca cgcgatatgg agcgccgtct
    64621 gtttatcgcc cgccgccgca ttgaaaagcg tctcgaagcc gacaaagact tctacgtctg
    64681 tagcctgtcg aatctggtga acatctataa aggtctgtgt atgccggcgg atctgccgcg
    64741 cttttatctg gatcttgcgg acctgcgtct ggaatcggcc atttgcctgt tccaccagcg
    64801 cttctccact aacaccgtac cgcgctggcc gctggcgcaa ccgttccgct atctggcgca
    64861 taacggtgaa atcaacacca tcaccggtaa ccgccaatgg gcgcgtgcgc gtacctataa
    64921 attccagacg ccgctgatcc ctgacctgca cgacgccgcg ccattcgtca acgaaaccgg
    64981 ttctgactcc agttccatgg ataacatgct ggaactgctg ctggcaggcg ggatggatat
    65041 catccgcgcc atgcgcctgt tagtgccacc cgcctggcag aacaacccgg atatggaccc
    65101 ggaactgcgt gccttctttg actttaactc catgcatatg gagccgtggg atggtccggc
    65161 gggcatcgtg atgtccgatg gtcgcttcgc tgcctgtaac ctcgaccgta acggtctgcg
    65221 tccggcacgc tacgtcatta ccaaagacaa gctcatcacc tgcgcctctg aagtcggtat
    65281 ctgggattac cagcctgacg aagtggtcga aaaaggccgc gtcgggccag gcgaactgat
    65341 ggtgatcgac actcgcagtg ggcgtattct gcactcggca gaaaccgatg acgatctgaa
    65401 aagccgccac ccttataaag agtggatgga gaaaaacgtc cgccgactgg taccgtttga
    65461 agatctgccc gatgaagaag tgggtagccg cgaactggac gacgacacgc ttgccagcta
    65521 ccagaaacag tttaactaca gcgcggaaga gctggactcc gtaattcgcg tactgggcga
    65581 aaacggtcag gaagcggtcg gttcgatggg cgatgatacc ccattcgccg tgctctccag
    65641 tcagccgcgc attatttacg actacttccg ccagcagttt gcccaggtga ctaacccgcc
    65701 aatcgacccg ctgcgtgaag cgcatgttat gtcgctcgcc accagtatcg gtcgtgaaat
    65761 gaacgtcttt tgcgaagcag agggccaggc gcaccgttta agctttaaat cgccgattct
    65821 gctctactcc gatttcaaac agctcacgac gatgaaagag gagcactacc gcgcagatac
    65881 gctggatatc acctttgacg tcactaaaac cacgctcgaa gcgacagtca aagagctatg
    65941 cgacaaagcc gaaaaaatgg tacgtagcgg caccgtgttg ctggtgctct ccgaccggaa
    66001 tatcgctaaa gatcgcctgc cggttccggc tccgatggcg gttggcgcga tccagacccg
    66061 tctggtcgat caaagcctgc gttgcgatgc caacatcatc gtcgaaaccg ccagcgcccg
    66121 cgatccgcac cacttcgccg tgttgctggg tttcggcgcg acggctattt atccgtacct
    66181 tgcctatgaa acgctgggcc gcctggtaga cacccacgcg attgccaaag attatcgtac
    66241 cgtgatgctc aactaccgta acggcatcaa caaaggcttg tacaaaatca tgtccaaaat
    66301 gggcatctcc accatcgcct cttaccgctg ctcgaaactg tttgaagcgg tcggtctaca
    66361 cgatgatgta gtgggcctgt gcttccaggg cgcggtcagc cgcattggtg gggcaagctt
    66421 tgaagacttc cagcaggatc tgctgaatct gtcgaaacgt gcctggctgg cgcgcaagcc
    66481 gattagccag ggcggcctgc tgaaatacgt ccacggcggc gaataccacg cctacaaccc
    66541 ggacgtggtg cgcacgctgc aacaggcggt acaaagcggc gagtacagcg actatcagga
    66601 atacgcgaag ctggttaatg agcgtccggc aaccacgctg cgcgatctgc tggcaattac
    66661 gccgggtgaa aacgcggtca acattgctga tgttgaaccg gcaagcgaac tgtttaaacg
    66721 ctttgatacc gccgcgatgt ctatcggcgc gttaagcccg gaagcccacg aggcgctggc
    66781 ggaagcgatg aatagcatcg gcggtaactc gaactccggt gaaggcggcg aagacccggc
    66841 gcgctatggc actaacaaag tgtcgcgcat caagcaggtg gcttctggtc gcttcggcgt
    66901 tactccggcg tatctggtca atgccgacgt cattcagatt aaagtcgccc agggcgcgaa
    66961 gccgggcgaa ggtggtcagt tgccgggcga caaagtcact ccttacatcg ccaaactgcg
    67021 ctattcggtg cccggagtga cgctgatctc cccgccgccg caccacgata tctactctat
    67081 cgaagactta gcgcagctta ttttcgacct caagcaggtt aacccgaaag cgatgatctc
    67141 cgttaagctg gtgtccgaac caggcgttgg caccatcgcg actggcgtgg cgaaagctta
    67201 tgcggacttg atcaccatcg cgggctatga cggcggcacc ggcgcaagcc cgctgtcgtc
    67261 agtgaaatat gcaggctgcc cgtgggaact cggtctggtg gaaacccaac aggcgctggt
    67321 tgccaacggc ttgcgtcata aaattcgttt gcaggttgat ggcggcctga aaacgggtgt
    67381 cgatatcatc aaagcggcga ttctcggcgc agaaagcttc ggcttcggca ctggcccaat
    67441 ggtggcgctc ggctgtaaat atctgcgtat ttgccacctg aacaactgcg ccacgggtgt
    67501 agcaactcag gatgacaaac tgcgtaagaa tcactatcac ggcctgccgt tcaaggtgac
    67561 taattacttt gagtttatcg cccgtgaaac ccgcgagctg atggcacagc tgggagtaac
    67621 gcgtctggtg gatctgattg gtcgcaccga cctgctgaaa gagctggacg gtttcaccgc
    67681 caaacagcag aaactggcgc tgtcgaagct gctggagact gccgagccac atgcaggtaa
    67741 gtcactctac tgcactgaaa acaacccgcc gtttgataac ggcctactga acgcgcagtt
    67801 gctgcaacag gcgaaaccgt ttgtcgatga acgccagagc aaaaccttct ggttcgatat
    67861 ccgcaacacc gaccgttctg tcggcgcgtc gctttcaggc tatatcgccc agacgcacgg
    67921 cgatcagggg ctggcagccg atcctatcaa agcgtacttc aacggcaccg caggccagag
    67981 tttcggtgtg tggaacgcgg gcggcgtgga actgtacctg accggggatg ccaacgacta
    68041 tgtcggtaaa ggcatggcgg gcggcttaat cgccattcgt cctccggttg gttccgcctt
    68101 ccgcagccat gaagcaagca ttatcggcaa cacctgcctg tatggcgcga ccggtggtcg
    68161 tctgtatgcc gcaggccgcg cgggtgaacg tttcggcgtg cggaactccg gtgctatcac
    68221 cgtggtagaa ggcattggcg acaacggctg tgaatatatg acgggcggta ttgtctgtat
    68281 tctgggtaaa accggcgtta acttcggcgc gggcatgacc ggcggcttcg cttacgttct
    68341 cgatgaaagc ggtgatttcc gcaaacgcgt taacccggaa ctggtcgaag tcttaagcgt
    68401 cgacgatctg gcgatccatg aagagcatct gcgcggtctt atcaccgagc atgttcagca
    68461 taccggttct cagcgcggtg aagagattct ggcgaactgg tcaaccttcg ccacgaaatt
    68521 cgcgctggtt aaaccgaagt ccagtgatgt aaaagcactg ctgggtcacc gtagtcgtag
    68581 cgcagcagag ttgcgcgtgc aggcgcagta aggggtagca acaatgagtc agaatgttta
    68641 tcaatttatc gacctgcagc gcgttgatcc gccaaagaaa ccgctgaaga tccgtaaaat
    68701 tgagtttgtt gaaatttacg agccgttttc cgaaggccag gccaaagcgc aggctgaccg
    68761 ctgtctgtcg tgcggcaacc catactgcga gtggaaatgc ccggtacaca actacatccc
    68821 aaactggctg aagctcgcca acgaggggcg tatttttgaa gcggcagaac tgtcgcatca
    68881 gaccaacaca ctgccggaag tatgcggacg tgtctgcccg caagaccgtc tgtgcgaagg
    68941 ttcctgcacc ctgaacgatg agttcggtgc ggtgaccatc ggcaacattg agcgttatat
    69001 caatgataaa gcgttcgaaa tgggctggcg tccggatatg tccggcgtga agcaaaccgg
    69061 taaaaaagtg gcgattatcg gtgcaggccc ggcaggtctg gcgtgtgcgg atgtcctgac
    69121 gcgtaacggc gtaaaagccg ttgtcttcga ccgtcatcca gaaattggcg ggctgctgac
    69181 cttcggtatt ccggccttca agctggaaaa agaggtaatg acgcgtcgcc gtgaaatctt
    69241 caccggcatg ggtattgaat tcaaactcaa taccgaagtg ggccgcgacg tacagctgga
    69301 cgatctgctg agtgattacg atgccgtgtt ccttggcgtt gggacttatc agtcaatgcg
    69361 cggcgggctg gaaaacgaag acgccgatgg cgtgtacgca gcgctgccgt tccttatcgc
    69421 caacaccaaa cagttaatgg gctttggtga aacccgcgac gaaccgttcg tcagcatgga
    69481 aggcaaacgc gtggtagtac ttggcggtgg cgacactgcg atggactgcg tgcgcacgtc
    69541 tgtacgccag ggggcaaagc acgttacctg tgcctatcgt cgtgatgaag agaacatgcc
    69601 gggttcccgc cgcgaagtga aaaacgcgcg ggaagaaggc gtagagttca aattcaacgt
    69661 ccagccactg gggattgaag tgaacggtaa cggcaaagtc agcggcgtaa aaatggtacg
    69721 taccgaaatg ggcgaaccgg atgccaaagg ccgtcgccgc gcggagatcg ttgcaggttc
    69781 cgaacatatc gttccggcag atgcggtgat catggcgttt ggtttccgtc cacacaacat
    69841 ggaatggctg gcaaaacaca gcgtcgagct ggattcacaa ggccgcatca tcgctccgga
    69901 aggcaacgac aacgctttcc agaccagcaa cccgaaaatc tttgctggcg gcgatatcgt
    69961 ccgtggttcc gatctggtcg ttaccgccat cgccgaaggt cgcaaagcgg cagacggcat
    70021 tatgaactgg ctggaagttt aagctctgtc ccagcgccgc ctcctcagaa aagagtcggc
    70081 gctgaaattc tcctttcttt ctggaaatcg cctcgcataa tctgaagaaa accaatgtaa
    70141 tacgctcatc tggattgtta ccggtagatg cccggcgtat agctgattcc attattcaac
    70201 gcagcgccat ataaagggac agataatgga atcgctctca gagagaacct caacaggcta
    70261 ccagcaaatc cacaacggca ttattcatct ggtcgatagc gcccgaacgg aaacggtacg
    70321 tagcgttaac gcgctgatga ccgcgacgta ctgggaaatt ggccgacgga ttgtcgaatt
    70381 tgaacaaggt ggtgaggcca gagcagctta tggtgcacag ttgattgatc ggctatcgaa
    70441 ggatttaagt cgacggtata aacgtggggt ctctgcaaaa aacttacgcc aaatgaggct
    70501 tttttacctc ttttttcaac atgttgaaat tcgccagaca gtgtctggcg aattaacacc
    70561 attgggaatt ccgcagacac catctgcaga attcccatcg gcaaagattt ggcagacgct
    70621 gtctgccaaa ttgagaggct gtttgatatt tccacagctt ttgtattgca tctcctattt
    70681 tcggtttgta tattcatgca aataaacgaa gg
//
LOCUS       DAJTSA010000007        67671 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000007 DAJTSA010000000
VERSION     DAJTSA010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67671
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.007"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..228
                     /locus_tag="OO369_000496"
     CDS             <1..228
                     /locus_tag="OO369_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCR5980886.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            623..1262
                     /locus_tag="OO369_000497"
                     /pseudo
     CDS             623..1262
                     /locus_tag="OO369_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1365..1688
                     /locus_tag="OO369_000498"
     CDS             1365..1688
                     /locus_tag="OO369_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HCR5980887.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1755..3212)
                     /gene="nuoN"
                     /locus_tag="OO369_000499"
     CDS             complement(1755..3212)
                     /gene="nuoN"
                     /locus_tag="OO369_000499"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR5980888.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3219..4748)
                     /gene="nuoM"
                     /locus_tag="OO369_000500"
     CDS             complement(3219..4748)
                     /gene="nuoM"
                     /locus_tag="OO369_000500"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR5980889.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4912..6753)
                     /gene="nuoL"
                     /locus_tag="OO369_000501"
     CDS             complement(4912..6753)
                     /gene="nuoL"
                     /locus_tag="OO369_000501"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR5980890.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6750..7052)
                     /gene="nuoK"
                     /locus_tag="OO369_000502"
     CDS             complement(6750..7052)
                     /gene="nuoK"
                     /locus_tag="OO369_000502"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR5980891.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7049..7603)
                     /gene="nuoJ"
                     /locus_tag="OO369_000503"
     CDS             complement(7049..7603)
                     /gene="nuoJ"
                     /locus_tag="OO369_000503"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR5980892.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7615..8157)
                     /gene="nuoI"
                     /locus_tag="OO369_000504"
     CDS             complement(7615..8157)
                     /gene="nuoI"
                     /locus_tag="OO369_000504"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR5980893.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8172..9149)
                     /gene="nuoH"
                     /locus_tag="OO369_000505"
     CDS             complement(8172..9149)
                     /gene="nuoH"
                     /locus_tag="OO369_000505"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR5980894.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9146..11872)
                     /gene="nuoG"
                     /locus_tag="OO369_000506"
     CDS             complement(9146..11872)
                     /gene="nuoG"
                     /locus_tag="OO369_000506"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR5980895.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11925..13262)
                     /gene="nuoF"
                     /locus_tag="OO369_000507"
     CDS             complement(11925..13262)
                     /gene="nuoF"
                     /locus_tag="OO369_000507"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR5980896.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13259..13759)
                     /gene="nuoE"
                     /locus_tag="OO369_000508"
     CDS             complement(13259..13759)
                     /gene="nuoE"
                     /locus_tag="OO369_000508"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR5980897.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13762..15564)
                     /gene="nuoC"
                     /locus_tag="OO369_000509"
     CDS             complement(13762..15564)
                     /gene="nuoC"
                     /locus_tag="OO369_000509"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR5980898.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15658..16320)
                     /gene="nuoB"
                     /locus_tag="OO369_000510"
     CDS             complement(15658..16320)
                     /gene="nuoB"
                     /locus_tag="OO369_000510"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR5980899.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16336..16779)
                     /gene="nuoA"
                     /locus_tag="OO369_000511"
     CDS             complement(16336..16779)
                     /gene="nuoA"
                     /locus_tag="OO369_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR5980900.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17410..18348)
                     /gene="lrhA"
                     /locus_tag="OO369_000512"
     CDS             complement(17410..18348)
                     /gene="lrhA"
                     /locus_tag="OO369_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR5980901.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19267..20484
                     /gene="alaA"
                     /locus_tag="OO369_000513"
     CDS             19267..20484
                     /gene="alaA"
                     /locus_tag="OO369_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR5980902.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20568..21167
                     /gene="yfbR"
                     /locus_tag="OO369_000514"
     CDS             20568..21167
                     /gene="yfbR"
                     /locus_tag="OO369_000514"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR5980903.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21226..23058)
                     /locus_tag="OO369_000515"
     CDS             complement(21226..23058)
                     /locus_tag="OO369_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR5980904.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23145..23795)
                     /gene="hxpA"
                     /locus_tag="OO369_000516"
     CDS             complement(23145..23795)
                     /gene="hxpA"
                     /locus_tag="OO369_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR5980905.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23806..24300)
                     /locus_tag="OO369_000517"
     CDS             complement(23806..24300)
                     /locus_tag="OO369_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR5980906.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24383..24838)
                     /locus_tag="OO369_000518"
     CDS             complement(24383..24838)
                     /locus_tag="OO369_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR5980907.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25176..26378
                     /gene="ackA"
                     /locus_tag="OO369_000519"
     CDS             25176..26378
                     /gene="ackA"
                     /locus_tag="OO369_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR5980908.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26453..28597
                     /gene="pta"
                     /locus_tag="OO369_000520"
     CDS             26453..28597
                     /gene="pta"
                     /locus_tag="OO369_000520"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR5980909.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28787..30307
                     /gene="yfcC"
                     /locus_tag="OO369_000521"
     CDS             28787..30307
                     /gene="yfcC"
                     /locus_tag="OO369_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR5980910.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30340..30882)
                     /gene="yfcD"
                     /locus_tag="OO369_000522"
     CDS             complement(30340..30882)
                     /gene="yfcD"
                     /locus_tag="OO369_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR5980911.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30940..31491)
                     /gene="yfcE"
                     /locus_tag="OO369_000523"
     CDS             complement(30940..31491)
                     /gene="yfcE"
                     /locus_tag="OO369_000523"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR5980912.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31547..32191)
                     /gene="yfcF"
                     /locus_tag="OO369_000524"
     CDS             complement(31547..32191)
                     /gene="yfcF"
                     /locus_tag="OO369_000524"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR5980913.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32327..32974
                     /gene="yfcG"
                     /locus_tag="OO369_000525"
     CDS             32327..32974
                     /gene="yfcG"
                     /locus_tag="OO369_000525"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR5980914.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33031..33393
                     /gene="folX"
                     /locus_tag="OO369_000526"
     CDS             33031..33393
                     /gene="folX"
                     /locus_tag="OO369_000526"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR5980915.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33414..34307
                     /locus_tag="OO369_000527"
     CDS             33414..34307
                     /locus_tag="OO369_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR5980916.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34355..35245)
                     /gene="rpnB"
                     /locus_tag="OO369_000528"
     CDS             complement(34355..35245)
                     /gene="rpnB"
                     /locus_tag="OO369_000528"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR5980917.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35442..36215)
                     /gene="hisP"
                     /locus_tag="OO369_000529"
     CDS             complement(35442..36215)
                     /gene="hisP"
                     /locus_tag="OO369_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR5980918.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36223..36939)
                     /locus_tag="OO369_000530"
     CDS             complement(36223..36939)
                     /locus_tag="OO369_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5980919.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36936..37622)
                     /gene="hisQ"
                     /locus_tag="OO369_000531"
     CDS             complement(36936..37622)
                     /gene="hisQ"
                     /locus_tag="OO369_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR5980920.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37712..38494)
                     /gene="hisJ"
                     /locus_tag="OO369_000532"
     CDS             complement(37712..38494)
                     /gene="hisJ"
                     /locus_tag="OO369_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR5980921.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38715..39497)
                     /gene="argT"
                     /locus_tag="OO369_000533"
     CDS             complement(38715..39497)
                     /gene="argT"
                     /locus_tag="OO369_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR5980922.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39763..40332)
                     /gene="ubiX"
                     /locus_tag="OO369_000534"
     CDS             complement(39763..40332)
                     /gene="ubiX"
                     /locus_tag="OO369_000534"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR5980923.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40427..41944)
                     /gene="purF"
                     /locus_tag="OO369_000535"
     CDS             complement(40427..41944)
                     /gene="purF"
                     /locus_tag="OO369_000535"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR5980924.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41981..42469)
                     /gene="cvpA"
                     /locus_tag="OO369_000536"
     CDS             complement(41981..42469)
                     /gene="cvpA"
                     /locus_tag="OO369_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR5980925.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42728..43390)
                     /gene="dedD"
                     /locus_tag="OO369_000537"
     CDS             complement(42728..43390)
                     /gene="dedD"
                     /locus_tag="OO369_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR5980926.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43380..44648)
                     /gene="folC"
                     /locus_tag="OO369_000538"
     CDS             complement(43380..44648)
                     /gene="folC"
                     /locus_tag="OO369_000538"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR5980927.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44718..45632)
                     /gene="accD"
                     /locus_tag="OO369_000539"
     CDS             complement(44718..45632)
                     /gene="accD"
                     /locus_tag="OO369_000539"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR5980928.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45788..46447)
                     /locus_tag="OO369_000540"
     CDS             complement(45788..46447)
                     /locus_tag="OO369_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5980929.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPVVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46530..47342)
                     /gene="truA"
                     /locus_tag="OO369_000541"
     CDS             complement(46530..47342)
                     /gene="truA"
                     /locus_tag="OO369_000541"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR5980930.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47342..48355)
                     /locus_tag="OO369_000542"
     CDS             complement(47342..48355)
                     /locus_tag="OO369_000542"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5980931.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48421..49557)
                     /gene="pdxB"
                     /locus_tag="OO369_000543"
     CDS             complement(48421..49557)
                     /gene="pdxB"
                     /locus_tag="OO369_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR5980932.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49656..50651
                     /gene="flk"
                     /locus_tag="OO369_000544"
     CDS             49656..50651
                     /gene="flk"
                     /locus_tag="OO369_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR5980933.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50648..51826)
                     /locus_tag="OO369_000545"
     CDS             complement(50648..51826)
                     /locus_tag="OO369_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5980934.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52081..53322)
                     /gene="fabB"
                     /locus_tag="OO369_000546"
     CDS             complement(52081..53322)
                     /gene="fabB"
                     /locus_tag="OO369_000546"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR5980935.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53481..55487
                     /gene="mnmC"
                     /locus_tag="OO369_000547"
     CDS             53481..55487
                     /gene="mnmC"
                     /locus_tag="OO369_000547"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR5980936.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55608..55886)
                     /locus_tag="OO369_000548"
     CDS             complement(55608..55886)
                     /locus_tag="OO369_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR5980937.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55920..56468)
                     /gene="epmC"
                     /locus_tag="OO369_000549"
     CDS             complement(55920..56468)
                     /gene="epmC"
                     /locus_tag="OO369_000549"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR5980938.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56468..57277)
                     /locus_tag="OO369_000550"
     CDS             complement(56468..57277)
                     /locus_tag="OO369_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR5980939.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57277..58101)
                     /gene="mepA"
                     /locus_tag="OO369_000551"
     CDS             complement(57277..58101)
                     /gene="mepA"
                     /locus_tag="OO369_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR5980940.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58105..59190)
                     /gene="aroC"
                     /locus_tag="OO369_000552"
     CDS             complement(58105..59190)
                     /gene="aroC"
                     /locus_tag="OO369_000552"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR5980941.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59225..60157)
                     /gene="prmB"
                     /locus_tag="OO369_000553"
     CDS             complement(59225..60157)
                     /gene="prmB"
                     /locus_tag="OO369_000553"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5980942.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60323..60874
                     /gene="smrB"
                     /locus_tag="OO369_000554"
     CDS             60323..60874
                     /gene="smrB"
                     /locus_tag="OO369_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR5980943.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60978..61868)
                     /locus_tag="OO369_000555"
     CDS             complement(60978..61868)
                     /locus_tag="OO369_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR5980944.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61855..62379)
                     /locus_tag="OO369_000556"
     CDS             complement(61855..62379)
                     /locus_tag="OO369_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5980945.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62376..62846)
                     /locus_tag="OO369_000557"
     CDS             complement(62376..62846)
                     /locus_tag="OO369_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5980946.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62843..63391)
                     /locus_tag="OO369_000558"
     CDS             complement(62843..63391)
                     /locus_tag="OO369_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5980947.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63396..64118)
                     /locus_tag="OO369_000559"
                     /pseudo
     CDS             complement(63396..64118)
                     /locus_tag="OO369_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64138..64860)
                     /locus_tag="OO369_000560"
     CDS             complement(64138..64860)
                     /locus_tag="OO369_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5980948.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64920..66074)
                     /gene="yfcU"
                     /locus_tag="OO369_000561"
                     /pseudo
     CDS             complement(64920..66074)
                     /gene="yfcU"
                     /locus_tag="OO369_000561"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(66023..66778)
                     /locus_tag="OO369_000562"
     CDS             complement(66023..66778)
                     /locus_tag="OO369_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5980949.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDMKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDRD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKNGSGVATMPGGRYHH"
     gene            complement(66860..67423)
                     /locus_tag="OO369_000563"
     CDS             complement(66860..67423)
                     /locus_tag="OO369_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5980950.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16923 a        18284 c        16784 g        15680 t
ORIGIN      
        1 gaaagcaatt ctgacagcgc cccctatacg ttattaaaaa actatgctga cagatttaaa
       61 aaatattcag cagaagagca gtacgaaatt gattttcaac atcgactggt aaacagaaat
      121 tgttatttag ataaatatgg cgatgcacga atcaatgctt cttatacaca attagaaata
      181 aaacactcac aacccaaaaa tagagcatcc ggcaaaagag tgagttaaca tcattgttaa
      241 tcaattcgac cagtcagtaa atatgctgac tggtcgaaca taatttgcta aaaagtcatc
      301 actgactgat gtcagtccca ccatctgcca gttcaatcta cgggtatttc cagcatatcg
      361 cggcacgcga ttgctggtct gttgatgtag tggattgtag ttgctgactt ttcttaccct
      421 cagatttctg ccccacttgt caattcaatc tgccaataaa taacataatt atatttttat
      481 tcctcacaga aattcctttt taaaaatagc gaacccactg taaaaaacac tttttgtata
      541 cctctaccaa gcgtaataaa ataaacgctc ataattcatt ataaataata atgaaataac
      601 gccttttatt gataatttac atatgaaaaa attttccttt tgttatatta tttctgtttt
      661 cactccctct gattattttt accaaccctg ggtcactgca ttatcgccaa cgccccgaca
      721 cgtaagtcct gagcaattag aaaaaacagt tcgttatctt acacaaactg tgcatccacg
      781 taatgccggc aatattgata atctgaatag gtccgcagag tatataaaag agttctttgt
      841 cagtagcggt gccagagtta ccgcgcaggg cgtccccgat tggctattac gactctgcgc
      901 ctggaagcca gaattaccct tacccggcaa tgtcctggct ttatcccgat cggggagact
      961 ttattgctgt ggtcggcaga atgcaggata tcaacgccgt tcgtcaggta aaagcggcat
     1021 tgttgtcatc ccgggattta tctgtttatt ctatgaatgc cccagggttt attcccggta
     1081 ttgatttttc tgatcacctc aattattggc aacacgatat tcccgccgta atgattactg
     1141 acaccgcttt ttatcgtaat aaacaatacc acttgcccgg tgataccgca gacagattga
     1201 attatcagaa aatggctcag gtggtagatg gtgttataac cttgttatac aacagtaaat
     1261 aataaaaatt tctcgaggcg ggtccagaaa cagcgacatg tacattttat tttccctcgt
     1321 ctgttttaag atgcgaaaca taaaattcaa tggaggaaaa ataaatggga atgattggct
     1381 attttgcaga aattgactca gaaaagatta accaactgct tgagtctact gaaaaacctt
     1441 tgatggataa tatacatgac acgctttcag gattacgccg actggaatca ctggatcgaa
     1501 gtgagctgcg taaacagttt tccattaagc gactcaatga aatggagatt tatccgggag
     1561 tgaccttttc agaagagtta gaggggcaac tctttgcctc gatcatgctt gatatggaaa
     1621 agctgattag cgcgtatcga cgtatgctgc gacaagggaa tcatgcgctg acggttattg
     1681 ttgggtgatg gtgtcagagc gccaaaaaaa accgccgaac atgtcggcgg ctttctgact
     1741 tacaaactaa cagtttacat cagcggcatt gctaaccgca caatactaat cagcggttgt
     1801 ggccatacac ccagcaccag taccaacagt gcagaaatca gcaccacgat accgcccgcg
     1861 ctgtactgcc agtttgacgg tgcatcgcga cccggttgtt ccggtgcgtg caggtacagg
     1921 ctcaccgcca cgcgcaggta gtagtagagg ccgattgccg aaccgacaac cacggcaccc
     1981 accagccacc acaagtgtgc ctggacaccg accgccagca cgtagaactt accgataaag
     2041 cccagcgtca tcgggatacc ggccagagac agcatcatca ccgtcatcac tgccgcgagg
     2101 atcggacgat gccagaacag accgcggtaa gagaacaggg aatcagcatc cgggccacga
     2161 tacgggctgg acatcaggct gaccacgccg aacgcgccga ggctgctgaa cagataaccg
     2221 gccaggtaaa cccctaccgc ttccatcgac atctcgccgg tttgcagcgc aatcagcgcc
     2281 accagcagat agccgaggtg agagatagat gagtaaccga gcagacgttt gatgttggtc
     2341 tggctcaacg ccatcaggtt accgaagatg atggaggcaa aggcgataat cgccagtact
     2401 acgcgaatcg cttcgctgtc acccaccggt gcgtagagga acagacgcat caccacaccg
     2461 aagatagcga ttttgctcgc cgtcgccagg aaagtggaaa ccggcgcagg cgcgccctgg
     2521 tatacgtctg gcgtccacag gtggaacggc accagagaga gtttgaagcc gaggccaaca
     2581 atcatcatgc cgaaacctgc cagcaacagc ggctcgttga gcataccgtc gccgaggttt
     2641 ttacccagcg cgacaaacga caggtcgcca gactgcgcat acaccagcgc cataccgaac
     2701 agcaggaaag aagacgctgc ggcagaaagg atggtgtatt tgatactggc ttccagcgaa
     2761 cgtttctggc ggaaagcgta accgaccagg ccaaacagcg gcaaagagat cagttcaata
     2821 ccgaggaaca gagacgccag atggtttgca ttcgccagca ggatcccgcc cagcgcggca
     2881 attaacacca acaggtagaa ctcatccttg ttgtcgttat agccttcaag ccacgggtag
     2941 gcgaaagtac aggtggcgag gctcgccaac aataccagcc cggtgtaaag catggcgaaa
     3001 ccatcaacgc gcatcagcgg cgtaacgtcc atagcgcccg cctggccaac aaaccagagc
     3061 gaaaccagcg ccgcgttaag cccaataacc gagagcgtag cgttgaggaa atgattgcgt
     3121 cgccacgcaa tggagagcat cacaaccacc accgtcaagc cgacgatcag caacggtagc
     3181 agtgcgatca ggttttgtgg agttattgtc atggcgattt acggccttgt agtagtaacg
     3241 gaattaacaa accactgctg gatattgcca atcgcggagt gcgaggtatc cagaatcggc
     3301 tgcggataga agcccagcag taccagcagc accaccagca acaggatcat aaacagctca
     3361 cgcagcgaca tccctggcag ttcctggctg gcaatctggc ttttcgcttt accgaagtaa
     3421 gcgcgatgca acatcgccag cgaataaaca gatgcaaaga ccagcccaaa ggtagagata
     3481 acggtaatca ccgggacaac ctggaagctg ccgaacagaa tcataaattc gccgacgaag
     3541 ttaccggtgc caggcatccc aagcgttgcc accgcaaaga acagcgacag tgctggcagc
     3601 catttcatct tgctccacag accgcccatc atgcgcatgt cgcgggtatg gatacgttca
     3661 taaagctgac cgcagagaat aaacagaccc gccgccgaca agccgtgcgc aatcatctgg
     3721 attaccgcgc cctggtaggc caactggctg ccggtgtaga ttgcaatcag cacgaagccc
     3781 atgtgggaaa ccgaggtgta ggcgatcaga cgtttgatat cggtctgagc gaaggtcatc
     3841 cacgcaccgt agaagatgcc gataacaccc agccacatag cgattggcgc gaactctgcc
     3901 gacgcgttcg ggaacagcgg cagggagaaa cgcaacaaac cgtaagcggc ggttttcagc
     3961 aagatccccg cgaggtccac ggaaccggcg gtcggagcct gggagtgcgc atccggcagc
     4021 cagccatgca gcggaaccac cggcattttg actgcgaagg cgatgaagaa gcccagcatc
     4081 aacagatatt ccacaccact ggacattggc gtattcagca gctcttcata gttgaaggtc
     4141 cagacgccgg tcgcattgta gtgaacaaaa accagcgcca ggatggcgat caacatcacc
     4201 agaccactcg cctgggtgta aatgaagaac ttggttgccg ccgtgatacg cgttttaccg
     4261 tcagaggctt tatgccccca cagtgcgatc aggaagtaca tcggcaccag catcatttcc
     4321 cagaagaaga agaacaggaa catgtcgatg gcaaggaaca cgccgataac gccgcccagg
     4381 atccacatca ggttgaggtg gaagaagccc tgatattttt cgatctcttt ccacgaacat
     4441 agtaccgcca gcacaccgag cagaccggtc agcacgacca tcagcagcga cagaccgtca
     4501 atggcgagat gaatagagat accaaaacgc gggatccacg gcatgtcgaa ttcagactgc
     4561 cactgcggaa ttccggcgga ttgcgtcagt gaataaccgc cctgcaacca cagttgcagc
     4621 gacagcgcca gcgtcaatcc catggtgatc agcgcgatcc agcgtggcac cttgacgcca
     4681 aagcgttcgg tctgccagca caggaagccg ccaataaagg gaattaatat tagccagggt
     4741 agtaacatgg cgatctttat tccttgtaaa agtcccgtca ggaccggatt ttcaacgaat
     4801 tctcacgaca gaaaattctc ttcggactgg gggtagtgtc ggatgcgccg cgaaggcagc
     4861 ttatccgacc tacgttctta tatgcccagg ggcaatccca caatccttaa ctcaacgcag
     4921 taccatcaac agtgccagca ccacgaccgc accgatgctc atggatgcca cataccagcg
     4981 cagataaccg ttctcgctta acagcagacc tttacctgca aagcgggaaa gaaccgccgg
     5041 gatgttcatc attgagttca gcggatcgcg tttcagcaac caggcaatac ccaggaacgg
     5101 cttgacgaac actttgtcgt acagccagtc aaagccccaa gcgttgtacc accaggtact
     5161 cagcaggcgg cccggcgcac tgttggcgat ggaggtcacc agagaacgtt tacccagcca
     5221 cagccaggct gccagcagaa tgccgaccac cgcgaccacg ccagaggtaa tttccagggt
     5281 caacatgctg ccgtgcgcca gttccgtcgt ttgcggaagc acgccctgca gcggcggtac
     5341 aatcagtgcg ccaacgaagg tggaaaggat cagcagcaca atcagcggca ggctgtgagt
     5401 tacccctttc acggcgtgag cgtgaatttg ttcttttccg tggaagacga tgaaaatcat
     5461 acggaaggtg tagagcgagg tcataaacgc accgaccaga cctgccacca tcagattgat
     5521 atgaccattc gccatcgcac ccgcgaggat ctcatcctta ctgaagaagc ccgcagtgac
     5581 cagcggcagt gccgacagtg ctgcgccgcc caccaggaag cagagataaa ccagcggaat
     5641 agatttacgc agaccgccca tcttgaagat gttctgttcg tgatggcagg ccagaatgac
     5701 ggaaccggat gccaggaaca gcagcgcttt aaagaacgcg tgagtcatca agtggaaaat
     5761 cgccgcatcc catgcctgca cgccaagcgc gaggaacatg tagccaatct ggctcatggt
     5821 agagtaagcg agaacacgtt tgatgtcggt ctgtaccagc gcggcaaaac cggccagcag
     5881 cagcgtaacc gccccgacaa tacccaccag atgcagaact tccggcgtca tcaggaacag
     5941 gccgtgggta cgggcgatca ggtagacacc cgcggttacc atcgttgcgg cgtggatcag
     6001 cgcggagaca ggcgtcgggc ccgccatcgc gtcagcaagc catgtctgca acggcaactg
     6061 cgcagattta ccgaccgcac cgcccagcag catcagcgtc gcccacatca gcatgttatt
     6121 gccgtcagca aagtgcgctg gtgccagttc caccatatcg cggaagttca gggtgcccag
     6181 ttcgttgtaa agaatgaaca gtgcgaaagc gaggaaaacg tcacccacac gggtcacgac
     6241 gaacgctttc attgctgctg cgccattctt cggatcggtg taatagaaac cgatcagcag
     6301 ataggagcac aggcccacgc cttcccagcc gaggtacatc agcagcaggt tatcggcgag
     6361 aaccagaact accatgctgg cgatgaacag gttggtgtag gcgaagaagc gagagtagcc
     6421 ctcttcaccg cgcatgtacc aggaggcgta catgtgaata aggaaaccca caccagtgac
     6481 caccgagagc atggtcagcg acaggccgtc cagcaccagg ttaaaaccga tgttaaagtc
     6541 gcctaccgac atccacgtcc acagcggctg gctgtatgcc tgctcgccgt tagcgaagaa
     6601 atcaacgccg ataaaggcgg ttaccagcgc cgccaggccc acagagccaa cgccgacgat
     6661 cgccgaaacg ttttcagacc agcgcccacg ggagaacgcc agcaggacga agccaatcaa
     6721 tggcaaaata atggttaagg caagcatgtt catccgcgca tctcacttac tgaatcgatg
     6781 ttcaggttct ggcgacgacg gtgaagttgc agcagcagcg caaggccgat actcgcttct
     6841 gccgccgcga ggctgatggc gagaatgtac atcacctgac cgtcggtctg accccagtag
     6901 cttccggcga ccacaaaggc cagcgcggag gcgttaatca tgatttccag accaatcagc
     6961 ataaacagca gattgcgacg gataaccaga ccggttaagc caagaacgaa taagattgcc
     7021 gcgaggatca gtccatgttg taaggggatc atgcgtgctc ctccgttttt cttttcgcgc
     7081 tgtcgtcttt acgattgctc agcacttcac ccgcacgctc ttcacgaccg acgtggaagg
     7141 ccacaaccag acccgcgagc agcagcatag aagccagttc caccgccagt acgtaaggcc
     7201 cgaacagcgt aatacccact gctttagcac tgattggcgt accgtcgata ccctgatcgt
     7261 taacaccgag gatggcgtaa acaatcacca ccagcatgat ggccgacaaa attgccgggc
     7321 caatccacac ctgcggtttc agccactggc gttcctgttc gatttctgaa ccgcccaggt
     7381 tgagcatcat caccacgaac acgaacagca ccataatggc acccgcgtag acgataattt
     7441 ccagcgcacc ggcgaagtaa gcgcctagtg agaagaacac cccggaaatc gccagcagcg
     7501 aaataatcag gtacagcagt gcgtgtaccg gattggtatg ggtgatcact cgcaaggtcg
     7561 caagtatggc tatcaggcca cagatataaa aagcgaactc cattgcccct ctccttacgg
     7621 taacaggctc ttgacgtcga taggcttggc ttcgttctct gcttcgccct tatctttgcc
     7681 gtcgattgcc atacctgcca tccggtagaa gttatattcc gggtatttgc ccggaccgga
     7741 gatcagcaga tcctctttct cgtaaaccag atcctggcgc ttgtattccc ccatttcgaa
     7801 atccggggtt aactgaatcg ccgtggtcgg acaggcttct tcgcacagac cacagaaaat
     7861 gcagcgtgag aagttaatgc ggaaaaattc cggataccag cgaccgtctt tggtttctgc
     7921 tttttgcagc gagatacagc cgaccgggca ggctaccgca cagaggttac aggctacgca
     7981 acgctcttcg ccgtccgggt cgcgggtcag aacgatacga ccacgataac ggggcggcag
     8041 atagaccggc tcttccgggt acattcgcgt ttcgcgtttg gcgaacgcgt gcaggccgat
     8101 catccagata ctacgaacct gggtgccgaa acctactaac aattctttta aggtcatggt
     8161 cttattgccc cttattgcgc ctgccagaga atgacagccg ccgttaccag caagttgatc
     8221 agcgtcagcg gcaggcagat tttccagccg aaggacatta cccggtcata acgcggacgc
     8281 ggtaacgacg cacgaatcaa aatgaacatc atcataaaga acgcggtttt cagcgcgaac
     8341 cagatgaatg gcggtaacaa cgggccttgc cagccaccga agaacagcgt caccatcaat
     8401 gcagagatgg tcacaatccc gatgtactca cccacgaaga acagaccgaa cttcataccg
     8461 gaatattcaa tgtggtaacc atccgccagt tcctgctcgg cttccggctg gtcaaacggg
     8521 tgacggtgac acaccgccac gcccgcgatg gcaaaggtaa taaaaccaaa gaattgcggg
     8581 atgacgttcc acacatgcgc ctggctgttg acgatgtcgg tcatgttgaa tgaaccggcc
     8641 tgcgccacca cgcccatcag ggaaagcccg aggaacactt cgtagctcag ggtctgcgca
     8701 gaagcacgca tcgcacccag cagcgagtat ttgttgttac ttgaccagcc cgcaaacagc
     8761 accgcgtaaa ccgccagacc tgccatcatc aggaagaaca aaatcccgat gttcaggtcg
     8821 gcaaccaccc aacccggact gactggcaca attgcaaagg ccagcagcag cgaggtaaag
     8881 gcaatcatcg gtgccagggt aaagatgacg cgatccgaga atttcgggat ccagtcttct
     8941 ttaaagaaca ttttgatcat gtccgcaacc agctggagcg aaccgcccca gccaacacgg
     9001 ttaggtccat aacggttctg gaacagaccc agcaggcgac gttcgccaaa gctcatgaat
     9061 gccccacagg tgacaaccac cagcaggatc accaccgctt tgaggatggt cagcaggatc
     9121 tcaatcagtt ccggtgatat ccaactcatt gttgtgcctc cttgagatcc tcaagatgcg
     9181 cgccagccag caccggagca atgccggaca tacccatcgg caagcccacc tgccctgccg
     9241 tcagtccttc ggcgatttca accggcagcg tgaccgtgtt gccatcgtaa ctaaaggaga
     9301 cgcgtgtacc tgcgttcaca cccaacttcg cggcatccgc tgggttgagt ttgatgtacg
     9361 gctgcggcat acggctctgg aagaccggag cacgctgtga caattcatcg ctgccaaaca
     9421 ggtggtagta cggcgcgata cgccatttcc cgtcctgcgg ctggaagcgt gccggtacgc
     9481 tggtgaagta atccagaccg ttttcgctgg tttcaaacag acgcacgccc ggatcgccaa
     9541 agcgcagttt gccgcccact tcgtcctgga atttgttcca cgcctgcggg gagttccagc
     9601 ccggcgccca ggcaaacggc acttgcgaac ggtgcgcggt cggctggttg ttaccttcca
     9661 tcgagaaggt gaacatggtg tcaatatcct gcggctgacg cggctcatga acgctgatat
     9721 tggcgcgcat ggcggtacga ccgctgtaac ggtgcggttc acgggccagt ttctgcccac
     9781 gaatacggaa tgtcgcatcc ggcgcagcat ctttgatacc cgccagttct gggattttcg
     9841 ccacaacagc gtcaattaca tggtcgagct gcgtccagtc cacttcacgg ctcagcaagg
     9901 tgctgtgcag cgagtgcagc cagcgccagc tttccagcat gacagttttg ctgtcgtaat
     9961 aagccggatc gtaaacctgg aagaaacgtt gggcgcgacc ttcattatta attaccgtac
    10021 cgtcgctttc agcaaagctg gcagaggaaa gcaccagatg ggcgttttcc atgatcgctg
    10081 tgcgttgatg atcaaccacc atcaccagcg gcgctttagc cagcgcagca ttcacgcggg
    10141 tagcagaagc gtaacgatgc agatcgtttt ccaacaccac caccgcgtcg gcgcgtccgg
    10201 tttccagttc ggttaacgct tcttcaagcg aaccgccacc cataatgccc agccccatgc
    10261 tgttgacgga acgggcaatc atggtgatac caacgtcagc gccgcgacct ttcagggctt
    10321 tcgcgacgtt agccgcagcc tgaatcacct ctatgctacc ggcgttcgtc ccggagataa
    10381 tcaacggttt cttcgcacct gccagtgcct gcacgatgac gtcgattttg ttttgcagct
    10441 caggttcgat accgtcaacc gctggtgcgg agttatccag cgcatgggcg atggcaaaac
    10501 ctaaacgcgc ctgatcttca accggtgcgc ggtaagtcca cgccgcgata tcatccagac
    10561 gagtgtcatc aacgttggta acaaacagcg gatgcttcgc acgttgaccg atgttgagga
    10621 ttgccgcaat ctgccagtca gccactttct gtgctgcagc catttcgcgc gctttacctt
    10681 tcaccgcctg acgcactgcc agtgcgacgc gcgcgccggt ctgggtaacg tcttcgccca
    10741 gcaccagtac cgcatcgtaa gattcgattt cgcgcagagc cggagtataa atgccgcctt
    10801 cacgcagcac tttcagcgcc agttgcagac gttcctgctc accgtgagcg ataccggtgt
    10861 agaagttttc ttcgcccacc agttcacgca gcgcaaagtt gctttccacg ctggcacgcg
    10921 gagaaccaat accgatcact ttcttcgact gacgcagaat atctgccgcg ccctgcattg
    10981 cctgttcggc gttgagggta atgaaatcat cgccacgacg ctgtactggc tgacgcggac
    11041 gatccttcag gttgacgtaa ccgtaaccga aacgaccacg gtcgcagagg aagtagtggt
    11101 ttaccgtacc gttgtaacgg ttttcgatac gacgcagttc gccgtaacgt tcaccggggc
    11161 tgatgttaca gccgatggaa cattgctggc agatgctcgg cgcaaactgc atatcccatt
    11221 tacggttgta acgctcggag tgcgttttgt cggtaaatac gccggtcggg caaatttcga
    11281 ccaggttacc ggaaaattcg ctttccagcg tgccgtcttc cgggcgaccg aagtagacgt
    11341 tgtcgtgcgc gccataaacg cccagatctg taccgtcagc gtaatctttg tagtaacgca
    11401 cacagcggta gcaggcgatg cagcggttca tttcgtgaga gatgaatggc cccaaatcct
    11461 gattacggtg ggtacgtttg gtgaaacggt agcgacggaa gctgtgtccg gtcatcacgg
    11521 tcatatcctg aagatggcag ttaccgcctt cttcacatac cggacagtcg tgcgggtggt
    11581 tggtcattaa ccactcgacc acactttcac ggaactgttt cgcttcttcg tcgtcaatgg
    11641 aaataaaggt gccatcggaa gccggtgtca tacaggacat caccaggcga ccacgcgtgt
    11701 cttccgcgtt ttggtattgc ttcaccgcac actggcggca agcaccgacg cttcccagcg
    11761 ccggatgcca gcaaaagtaa ggaatatcaa ggcccagaga cagacaagct tccagcaggt
    11821 tgtccgctcc gttgacctcg tattctttgc cgtctacatg aattgtagcc attagcatgc
    11881 ttccagtttt ctcggtcagg gaccgagcgt taatcgaaat tcggttacca gcgctctttc
    11941 agcaggttcg gctgaatccc attaatcaaa tgggtattgc tgaacggctg tttgattccc
    12001 gcctcaaatt cttcgcggaa atatttgatg gcgctctgta acggctccac tgcaccaggt
    12061 gcgtgggcac agaaagtttt acccgggcct aagaatcgac acagttgctc aagtgtttcg
    12121 atatcgcccg gctggccttc gccacgctcc agcgcacgca gaattttcac gctccacggc
    12181 agaccgtcgc ggcacggcgt acaccagccg caggactcac gggcgaaaaa ctcttccagg
    12241 ttacgcacca gcgacaccat gttgatctca tggtcaaccg ccatcgccag cgccgtaccc
    12301 agacggctgc ccgctttacc gatactttcg aattccatcg gcagatcaag gtgcgcttcg
    12361 gtcaggaagt cagtccccgc gccgcctggc tgccaggctt taaatttcag accatcacgc
    12421 ataccaccgg cgtaatcttc gaggatctcg cgtgcggtgg tgccgaacgg cagttcccac
    12481 agtcccggat ttttcacccg accggagaag cccatcagct tggtgccagc atctttactt
    12541 ttcgagatgt tctgatacca ctccacgccg ttagcgagga tcgccggaac gttacacagg
    12601 gtttcgacgt tgttgacaca ggtcggtttg ccccatacgc cggaggttgc cgggaagggg
    12661 ggtttcgagc gtgggttagc acgacgccct tccagggagt tgattaacgc tgtttcttcc
    12721 ccgcagatgt agcgccctgc cccggtatgg acgaacagtt cgaagtcgaa acctgttccc
    12781 ataatgtttt tgccaagcaa gcccgcttca gtggcttcgg caatggcgcg gcgcagattt
    12841 actgccgctt cgatatattc gccacgcagg aagatgtagc cacggtaagc tttcagcgca
    12901 aacgcggaga tgagcatacc ttccaccagc aggtgcggca gttgctccat caacaggcgg
    12961 tctttatagg tgcccggctc catttcatcg gcgttacaca gcaggtaacg gatgttcatg
    13021 gattcgtctt tcggcatcag gctccacttc aggccagtcg aaaagcccgc gccgccgcgc
    13081 cctttcagac cagcgtcttt tacctgatta acgatttcgt ccggagacag cccggtcagc
    13141 gccttacgcg ccccttcgta accgtttttg ctgcggtatt cgtccagcca cactggctgt
    13201 ttgtcatcgc gcagacgcca ggtcagcgga tgcgtttcgg gagtacggat aatgtttttc
    13261 atttataccg ctccagcagt tcagggatcg cttccggggt cagatgcgcg tgagtgtcct
    13321 catcgatcat catgtttggc cctttatcac agttccccag gcagcaagtt ggcagcagcg
    13381 taaagcggcc atcaaatgtc gtttgccctg gtttgatgtt cagctttttc tcgagcgccg
    13441 cctgaatacc ctgatacccg ttgatatgac agaccacgct gtcgcaataa cggatcacat
    13501 ggcgaccaac cggctggcgg aagatctgac tgtagaacgt tgccacacct tcgacgtcgc
    13561 ttgccggaat acccagcaca tcggcgatcg cgtggatcgc accatccggc acccagccac
    13621 gctgcttctg aacgattttc agcgcttcaa tggacgccgc acgcgggtct tcgtagtggt
    13681 gcatctcgtg ctcaatcgct tcacgctctg ccgcactcag ctcaaaagcc tcggtttgtg
    13741 gttgttgatt ctcgtgcata attagcggtc cacatctgac ataacaaaat cgatactgcc
    13801 cagataaaca atcaggtcag acaccaggct gccgcggatc gccgccggaa tttgctgcaa
    13861 atgcgcataa ctcggcgtgc ggatacgagt gcgataactc atggtgctac cgtcgctggt
    13921 caggtagtaa ctgttgatcc ccttggtcgc ctcaatcatc tggaaagatt cattggcagg
    13981 catcaccggc ccccacgaca cttgcaggaa gtgggtgatc agggtttcga tatgttgcag
    14041 cgtgcgctct ttcggcggcg gcgtggtcag cgggtgatcc gctttgaacg ggccttccgg
    14101 catgttgttg aggcactgct caagaatgcg cagactctgg cgcagctctt ccactttcag
    14161 cattacgcgg gtgtagcagt cagaaacacc accaccaacc gggatttcaa agtcgaagtt
    14221 ttcatagcca gaataaggac gcgccttacg cacgtcgaag tcgatcccgg tagcacgcag
    14281 gcccgcgcca gtggtgcccc actccagcgc ctctttcgcg ccataggcgg caacgccctg
    14341 ggaacgacct ttcagaatgg tattttgcag cgccgctttc tcgtaagacg ccagacgttt
    14401 cggcatccag tcgaggaact cacgcagcag acgatcccag ccgcgcggca ggtcgtgcgc
    14461 tacgccgcca atacggaacc acgccgggtg catacggaaa cccgtgatcg cttccaccag
    14521 atcgtaaatt ttctgacgat cggtaaaggc gaagaacacg ggcgtcattg cgccgacgtc
    14581 ctgaataaag gtcgagatgt acagcaggtg gctgttgata cggaacagtt cggagagcat
    14641 aacgcgaatg acgttaacgc gatccggcac ggtgatcccg gccagtttct ctaccgccag
    14701 cacgtaaggc atttcgttaa cgcagccgcc gaggtattca atacggtcgg tatacggaat
    14761 gtagctgtgc caggactggc gctcgcccat tttctccgca ccacggtggt ggtagccgat
    14821 gtctggtacg cagtcgacaa tctcttcgcc gtcgagttgt aaaacgatac ggaaagcccc
    14881 gtgcgccgac gggtggttcg gaccgaggtt gaggaacatg aagtcctcat tttcggtgcc
    14941 gcgcttcatc ccccactctt ccggtttgaa ggtcagggct tccatctcca gatcctgttt
    15001 ggctttggtc agctcaaacg gcgagaattc ggtagcgcgt gccgggtaat ctttacgcag
    15061 cgggtgacct ttccaggttt gcggcatcat gatgcggcgc aggttcgggt gaccgtcgaa
    15121 agtaatgcca aacagatccc aggtttcacg ctcataccag ttagcgttcg ggaacagttt
    15181 ggtgaaggtc ggtacgtgca ggtcgttttc tgccagcgcc accttcagca tgatgtcgcg
    15241 gttacgatcg atagaaatca gatggtagaa aacggaaaaa tccgcggcag gtaacccttc
    15301 gcggtgtgtg cgcagacgtt cgtccatgcc gtgtaagtca aacagcatga cgtaaggttt
    15361 cggcagtttc tttaagaaat cgccaacttc cagtaattgt tcacgcttga tccacacaac
    15421 gggaaccccg gtgcgagtcg cctgaacagt aaaggcatcc ggcccaaaac ggttgcgcag
    15481 ttcgccaatt accggatcat caagatgatc gcgggtctgc caggcgggtt cttgcgcggt
    15541 taagtcggtc atattgttca ccattgcaaa tggtccgtgg tgactgtcgg cgcagtaagc
    15601 ttcgcgaaat agatttagga ataagcgaag aaaaatccct ttgccgacag gcgcaaatta
    15661 aatctcgtca ggtgtacgca ggttggttac ggcaatgcgt tcaccgcgct tgcgttcgcg
    15721 ctctgattgc atattggcgc gataaacgcc ctgatcgcca accacccagg agagcggacg
    15781 acgttctttg ccgatagatt cctgcaacag catcagtgcc tgcatgtacg cttcaggacg
    15841 cggcgggcag cccgggatat acacatcaac cgggatgaat ttatcgacgc cctgcacaac
    15901 ggaataaata tcgtacatac caccagagtt ggcacaggca cccattgaga taacccattt
    15961 tggttccagc atctggtcat acagacgctg aataaccggt gccattttgg taaagcaggt
    16021 tcctgcaacc accatcaggt cagcctgacg cggcgaagca cgcaatactt ctgcgccaaa
    16081 acgcgccacg tcatgcaccg cggtaaacga agtcaccatc tcaacgtaac agcaggaaag
    16141 accgaagtta tacggccaaa ttgagttttt acgaccccag ttaaccatgt cattgagctt
    16201 gcccataaac acgtttttgt taacttcttg ctccagaggg tcggttacga tctcctgctt
    16261 ttgcaggggg taacggtcgt tctcaccgtt gggatctatg cgggtgagcg tataatccat
    16321 cttaatgcct cgcggttagc gttgacgatt agcgatactg ttcgtttccg ggttcatacg
    16381 ctcgcggcgt gaacgcgcgg gcgtccagtc cagcgcgcca atacgcacca gataaaccag
    16441 acctgccagt aacacaaaaa taaaaattgc agcttccaca aagcctaccc agccgctttc
    16501 gcggatagag gttgaccatg cgaacagata cagcgcttca acgtcgaaga taacgaagaa
    16561 catggccacc agataaaact tggcggacag gcgtaagcgg gcggagccga ccgagtcgat
    16621 accggattca aacggcacgt ttttcgacct cgcgcgtgcg cgaccgccta aaaaccaacc
    16681 gcctaccagc atcaggcaac acaggccaat ggcaacgata agaaagatag cgaatgccca
    16741 gtgatgagcg atgacttcag tggatgttga catactcatt gcttactcat caaaagtagc
    16801 gccagattca ctgctcttca cggcagatgg acgccacatc gattcatggg gaggaataaa
    16861 aaaaacctta caataactgt agaaattctt ttatacagct aattgatgtg gtcttttact
    16921 cctttctata accttttgtc aactttaaca aaagcttctt cacattagtt tacataatat
    16981 caacaccatt agcatttaat gccctttcac cccagatcct tgacgactcc aggataatta
    17041 gatgttgttg aatcgtgtcc gttgtgaagc aatggaaaaa atacgggtct attttgacag
    17101 gaatttgtgt cgattcctcc ccccaaaaga gagtattttc ttgatctgtg acacgctttt
    17161 gttattccat aacaaaaacg cagcaacaaa tttacgtatt ttttaacatc attgtagcag
    17221 gtgatttttt tcaggcgatt atttgtgcat tcgggacgtg aatctctggt gattgaaaaa
    17281 taaacagttt tgtacgttct gcactatgcg aggaaggatt tttactaaaa aaaagccgct
    17341 ggggtttaaa acacccccag cggctcgttt tttacactat tgtctcagga attatctatc
    17401 gtccgtcgat tactcgatat ccctttcaat caacaatgaa tcatcccctt ccggagcaga
    17461 cattgggctg tattgccacg gattatggta gctctccatc gcctgataga tcacctgggc
    17521 cagctcatta ttactggacg ggtcatagca cagcaaatat tcggtatcag gtagcggcgg
    17581 taagccatct actccactca gcacacgcag gtccgggctc atcatctcaa ccggccttgc
    17641 cgtcacgcca agaccggctt tcactgccgc acgaacggcc ggaagcgtcg aggcgacata
    17701 agccagtcgc catggaatat ctactttatt aagcgtcgcc agcaccatat cgcgaaacgg
    17761 acttggatca tccagcaata caagagggat cggctcgcct ttttgcaata cgtattccgc
    17821 tgcgcagtac cagtgtgttg gcgaggtacg caggttcaat gccttaaacg ctgaaggacg
    17881 atgggtggtt accatcaaat ccacttcctg ggattccagc atttctgcca tataggcatt
    17941 acgtttaacg cggacatcca gcgctaattt cggataaacc gaactcacgc gatttaacag
    18001 gaaaggtaag atcgtatcgg cagattcatc tgaagcaccg atagttaaca cgccctgaag
    18061 attactgaac attaatgatg agcaggcctc atcattaaaa cgcaggattt tcctggcgta
    18121 accaagaagt tgaatgccat gttcagttaa cagtttgttg cgaccgtgac gagcgaacag
    18181 ttctttccca acgagttgtt ccagacgctg catttgctga cttacggcgg actgagtacg
    18241 acacacagcg gcagctgcgg cagcaaaagt gttcagatcg gcaacagcaa caaatgttct
    18301 cagcagatcg aggtcgaggt taattatcgg acgatttgca cttatcatat attatcactt
    18361 actggcggct catactgagc tggttaatgc tgtgcacaca caaacaagca attccatttg
    18421 taatgtgcct ccctggcagt ttcatccgga acccggacga aagtaaaaat gcatatgagt
    18481 tgcactaaaa aagcgactca cattgttccg ttataatgcc tgaagtagat cacagaatat
    18541 atcttcaggg atcgcatatc tattaagtta ctcactcttt tctatttatg acatgcgcgt
    18601 gtttgtataa atgtaaatgt gagtccttgt tccactctcg tgcagcatcg ctggtcatac
    18661 gcgaacacgt acaaacagca atggtgaagc ggcatcaaga gcagggatcc gtcatttatg
    18721 cgagcatttt accccaaaaa ccttttattt ataagggtca ttgcgaatta tctgatgcaa
    18781 agttatgtta tgttaggcaa agtaatcttc tattattaat aagcacatca aaaccttttt
    18841 gaatattaaa taataattaa ttagcatcat cctcattcat taattccgct taacaatagt
    18901 ttcacacctt acccccattg cgacacctcg gaaatcatca cgctgttaaa ctctccgtaa
    18961 tgcggaaata tcatcatcgc gtttcattag tgaattctta aatgaggcat tttcacacaa
    19021 ttatcttaca gataaaaaaa ccagacttgc aattaagaat tagaacaatt cactatataa
    19081 cattgcatgt aaagcatata agcctcatta tttgtcatta ttaagttatt aacagcacaa
    19141 tcgagccttc ccctctggca aaatcttatt ctgcagacct tcaaaacacc gtcctggggg
    19201 agtacattgt tctaagctga cttccacggc agggagtggc gataacagca aaaaaggtca
    19261 agattcatgt cccccattga aaaatccagc aaattagaga atgtctgtta tgacatccgt
    19321 ggtccggtgc tgaaagaagc aaaacgcctg gaagaagaag gtaacaaggt actgaaactg
    19381 aacatcggca acccagcccc attcggtttt gacgcgccag atgaaatcct cgttgacgtg
    19441 atacgcaacc tgcctaccgc tcaagggtat tgcgattcca aagggcttta ctccgcgcgt
    19501 aaagccatca tgcagcacta ccaggctcgt ggcatgcgtg atgttaccgt ggaagatatt
    19561 tacatcggca atggtgtatc ggagcttatc gttcaggcaa tgcaggcatt gctgaacagc
    19621 ggggacgaaa tgttggttcc tgcaccagat tacccactct ggaccgcggc ggtttcgctt
    19681 tccagcggta aagcggtgca ttatctttgc gatgaatcct ctgactggtt cccggacctc
    19741 gatgatattc gcgctaaaat tacgcctcgt acgcgtggga ttgttattat caacccaaat
    19801 aacccaaccg gcgcggtata ttccaaagag cttttaatgg agattgtgga gattgcacgt
    19861 cagcataatc tcattatctt cgccgatgaa atttatgaca aaattctcta cgacgatgct
    19921 gagcatcact caattgcgcc gctggcacct gacctgctga ccattacctt taacggactg
    19981 tcgaaaacgt accgcgttgc aggcttccgt caggggtgga tggtgttgaa cgggccgaaa
    20041 aaacacgcca aaggctacat cgaaggtctg gaaatgctgg cttcaatgcg cctgtgtgct
    20101 aacgttcctg cgcaacacgc cattcagacc gcgctgggtg gttatcagag catcagtgaa
    20161 tttattaccc ctggcggtcg tctttatgag cagcgtaacc gcgcgtggga actgatcaac
    20221 gatattccgg gcgtttcctg cgtgaaacct cgtggtgcgc tgtatatgtt cccgaaaatc
    20281 gacgccaaac gctttaacat tcatgacgat cagaaaatgg tgctggattt cctgttgcag
    20341 gaaaaagttc tgttggtgca agggacggca ttcaactggc cgtggccgga tcacttccgc
    20401 attgtcacgc taccgcgtgt cgatgatatc gagctgtctt tgagcaagtt cgcgcgtttc
    20461 ctttctggtt atcatcagct gtaatcttaa tttcactgcc ggagattgca tccggcagtg
    20521 ttatcccgcc acaatgacct gatgatgtca tcatacgtaa ggtcactatg aaacagagcc
    20581 atttttttgc ccatctctcc cgcctgaaac tcattaaccg ctggccgcta atgcgcaacg
    20641 tgcggacgga aaatgtgtcc gaacacagtt tgcaggtagc gatggtcgcc catgcgctgg
    20701 cagctatcaa aaatcggaaa tttggcggta atgtcaacgc cgaacgtatc gctttactgg
    20761 cgatgtacca cgatgcctca gaagtgctca ccggcgatct ccctactccg gtgaaatact
    20821 tcaatgcgca aatcgctcag gagtacaagg ctattgaaaa aatcgctcag cagaaactgg
    20881 tcgatatggt tccggaagaa ctgcgggata tctttgcgcc gttaattgac gagcatgcat
    20941 atagcgatga agaaaaatcg ctggtgaaac aggcagatgc actgtgtgca tatctgaaat
    21001 gtctggaaga actcgcggcc ggaaataatg aattcttgct ggcaaaaacg cgactggaag
    21061 cgacgcttga agcgcgtcgc agccaggaga tggactactt catggaagta tttgttccca
    21121 gcttccatct ttcgctcgat gagattagcc aggattcacc gctgtaagca gccggagtct
    21181 gcgtcgcatc agtcaataag cgccggatgc gacatcaggc tcttgtcaaa acggaaacag
    21241 catcgggatc atcaccacac aaaccgccat cacgataatg gtgaacggta cccccaactt
    21301 cacaaagtca ctgaagctgt aatttcccgg acctaaaacc agggtgttaa caggtgaaga
    21361 caccggtgtc ataaaggcgg cggatgctgc catcgccacg accatcgcaa atggataagg
    21421 cgacactccc atcgttttgg cagcagccag cgcaatcgga gccatcaaca ccgccgtcgc
    21481 ggtattagag ataaacagcc caataaccgc cgacaagaca aacaaacacc ccagcatcat
    21541 atgtggccca taaccgccgc caatatccat cagccctttc accgccagcg cgacacctcc
    21601 cgttttctgt aatgccacag caaacggcat catcccaacg atcaaaataa tgctcggcca
    21661 gtgaatggat ttataggcgc tttcagcatc tatacagcgg aatttcccca tcagcaggca
    21721 ggcgatgata gcggcaacag gattaggaat ttcatttgtc agcattaacg ccaccattag
    21781 caccagacag aaaatggcat ggggtgcctg gctgtgcgcg ggcgatgctt cactcacctc
    21841 ttccggtaag ttcagcgcta cgaagtcgcg gccctgtttg gccagcatac cgatcagttt
    21901 ccagttaccc acaaccagga tgatatcgcc cagcagcaga ggctcatccg ccagcgaacc
    21961 ttccagcgcc acgccattgc gcttgagccc caccacattc agtccgtagc gggtacgaaa
    22021 accaatttcg cgcaccgatt taccaatcag ttctgactca ggaattaatg aaatctctgc
    22081 catgcccaca tcaagggcct ggtcagaaaa atactcgccg cgcagtacca tcggctccag
    22141 caattgctca ctacaaaatt gccggagatc gacatcagcc gcagacatat caataagcaa
    22201 aacgtcacgc gcgcgaaatt cagaaacccc attaacgttc acgataacgc gacgaaaacg
    22261 ccgccagcgt tcaacaccga tgacgttagc gccataacgc tcacgtaatt tgagatcatc
    22321 cagccgttga cctatcattg gcgatccggg gcgaatagcc agacgtcgcg cacgcccggt
    22381 cagtcgatat tcacggataa gatcgcgaaa ggttcgacgc gtccagcctt cgcgctgcgg
    22441 ggtctgggta tcccctttca gcatgaaacg catcactaac atatacaaaa tacccagcac
    22501 cagcacgacc aggccaatag gtgttacgct aaagaaactg aagccgtgat agccttcacg
    22561 cagcaattca ctgttgacta ccaggttcgg cggcgtcgcc accagcgtca tcatgccgct
    22621 aatcagcccg gcaaaactta acggcatcat cagacgtgac ggcgaagttt gcatacgcat
    22681 ggcaacgctt aacaccacgg gaataaagat agcgacaacg ctggttgaac tcataaacgc
    22741 gccaagcccc gcgacggtca gcatcaacaa aaccaacatt ttgatttcac tgttgcccgc
    22801 cactttgacc agccatgttc ccattacggt ggcaacaccg gtacggacca aaccatcgcc
    22861 aataataaac aaggcggcaa tcaggacaac gttaggatca gaaaagccgg aaaatacttc
    22921 ggggaccgtc agcgttccgc ttaatgcaaa cgcgacaata acaaacaaag cgaccgcatc
    22981 catacgcact ctgcccgtcg caaacaagac gatggcaacg gccagtaatg aaagaaccca
    23041 aatcaattca ccgttcacaa cttatccttg ttaattgagg gggatgactt gattctgcca
    23101 taaaaaagcc ccgacgagac ggggctaaat catgatcagg tgtttcactg aataataaca
    23161 tcgccatttg gctgtttggt cacagtaatt tgctccagac tgtggaggac caaatcgacc
    23221 tcattcaggc gcggggtatc tgccggagcg ttaaccgcaa tgacatggca acccgccgcc
    23281 aggccagaaa gcacgccagc gggagcatct tccaccacca cacactcctg cggcgcaagc
    23341 cccagcagct gcgcacctaa cagatacgca tcaggttctg gttttccgcg cttcactcgc
    23401 tcagcggtta caaacacctc tggtgcggga agccccgcta ttttatggcg cgctcgcgct
    23461 accggcatgg agccagaagt cacaatggcc cacggaatac ctgctttatt caaatgattg
    23521 agtaaggcga ttgccccagg aagcgcggta ataccttcgg tttccgtggc ctcgatttgc
    23581 tccagacgcg taaactcggc ggcaatatca gcctcggatt tgcccgccat aaaatggcgc
    23641 aaagaggtga tcgcctgttt accgtgaatg aaagccagca cctcttccgg cgctaaccca
    23701 tgacgtctgg cccagttgct ccacgcccgt tctaccgcag gcagggaatc caccagcgtt
    23761 ccatcaagat caaacagaaa acctttgcac cgcacgcggg cctcctcagg cgttgataat
    23821 ttgattaatt tcgttggcgc tcaaatggta ctgacgcggg caggcatgcc acacattaag
    23881 catgcgctga tatttttccc acattggcgt ctgggcgtta aaaccatgag tcccagcgtc
    23941 aaaatgggta tagcgccctt ccacattaac cataaagcgg acataaccga ggtaacgtgc
    24001 ttcagtggcg gcgtcaaagc cgaggaaggt gacacgacgt tcatcgatgg attgctggtc
    24061 ctgcaaatta gaccaggaaa catacaacgc atgatacata tccataatgt cgatgatagt
    24121 gcggcaggtt tcttctttca gctcgccaaa ctcgcgatcc aattcacgca tctgtaatcc
    24181 gtaaccacgc tcaataattg tttgcaggcg acggtaacgt tcagcatttg ccggatcgag
    24241 catagtcatc atcttgtact gattagacaa aataagacgt tgcgcgttgg tcatttccat
    24301 tgttgactcc tgtatcactc tactacggtg aaaaaaaaga aggctgagta tgccttcttt
    24361 tatatgcgta atcaggggtc aattacaaat catcaaggaa agttttatcc agttgtttga
    24421 aggcgcgctt aagcgtgtca gctaatgcct ggtaatcagg cttgccttca acgggtgcca
    24481 acacctgtcc agactcctgc aatttaccgc gaacttcata aaaccagtta aggattgcag
    24541 ggggtaatgg cgttacagaa cgcttgccca gccaccacaa tccctgcatg ggtaaactta
    24601 aggcgaacag tgcagtggca actgccggcc caagcagacc gcccagggca atctgccagc
    24661 agagagtaaa tacggcgatc ggcggcataa aacggatcgc ataacgcgtc atcttgataa
    24721 cgcgattttc gacaaagacc ggagcaaggc gtttttccag tggccacgtc tttgagtaat
    24781 gctgtccgcg gcgaaacaag ctaaaaaaat taacagaacg attatccggc gttgacatgc
    24841 ttcacctcaa cttcacatat aaagattcaa aaatttgtgc aaattcacaa ctcagcggga
    24901 caacgttcaa aacattttgt cttccatacc cactatcagg tatcctttag cagcctgaag
    24961 gcctaagtag tacatattca ttgagtcgtc aaattcatat acattatgcc attggctgaa
    25021 aattacgcaa aatggcatag actcaagata tttcttccat catgcaaaaa aaatttgcag
    25081 tgcatgatgt taatcataaa tgtcggtgtc atcatgcgct acgctctatg gctccctgac
    25141 gtttttttag ccacgtatca attataggta cttccatgtc gagtaagtta gtactggttc
    25201 tgaactgcgg tagttcttca ctgaaatttg ccatcatcga tgcagtaaat ggtgaagagt
    25261 acctttctgg tttagccgaa tgtttccacc tgcccgaagc acgtatcaaa tggaaaatgg
    25321 acggcaataa acaggaagcg gctttaggtg caggcgccgc tcacagcgaa gcgctcaact
    25381 ttatcgttaa tactattctg gcacaaaaac cagaactgtc tgcgcagctg actgctatcg
    25441 gtcaccgtat cgtacacggc ggcgaaaagt ataccagctc cgtagtgatc gatgagtctg
    25501 ttattcaggg tatcaaagat gcagcttctt ttgcaccgct gcacaacccg gctcacctga
    25561 tcggtatcga agaagctctg aaatctttcc cacagctgaa agacaaaaac gttgctgtat
    25621 ttgacaccgc gttccaccag actatgccgg aagagtctta cctctacgcc ctgccgtaca
    25681 acctgtacaa agagcacggc atccgtcgtt acggcgcgca cggcaccagc cacttctatg
    25741 taacccagga agcggcaaaa atgctgaaca aaccggtaga agaactgaac atcatcacct
    25801 gccacctggg caacggtggt tccgtttctg ctatccgcaa cggtaaatgc gttgacacct
    25861 ctatgggtct gaccccgctg gaaggtctgg tcatgggtac ccgttctggt gatatcgatc
    25921 cggcgatcat cttccacctg cacgacaccc tgggcatgag cgttgacgca atcaacaaac
    25981 tgctgaccaa agagtctggc ctgctgggtc tgaccgaagt gaccagcgac tgccgctatg
    26041 ttgaagacaa ctacgcgacg aaagaagacg cgaagcgcgc aatggacgtt tactgccacc
    26101 gcctggcgaa atacatcggt gcctacactg cgctgatgga tggtcgtctg gacgctgttg
    26161 cattcactgg tggtatcggt gaaaatgccg cgatggttcg tgaactgtct ctgggcaaac
    26221 tgggcgtgct gggctttgaa gttgatcatg aacgcaacct ggctgcacgt ttcggcaaat
    26281 ctggtttcat caacaaagaa ggtacccgtc ctgcggtggt tatcccaacc aacgaagaac
    26341 tggttatcgc gcaagacgcg agccgcctga ctgcctgatt tcacaccgcc agctcagctg
    26401 gcggtgctgt tttgtaaccc gccaaatcgg cggtaacgaa agaggataaa ccgtgtcccg
    26461 tattattatg ctgatcccta ccggaaccag cgtcggtctg accagcgtca gccttggcgt
    26521 gatccgtgca atggaacgca aaggcgttcg tctgagcgtt ttcaaaccta tcgctcagcc
    26581 gcgtaccggt ggcgatgcgc ccgatcagac tacgactatc gtgcgtgcga actcttccac
    26641 cacgacggcc gctgaaccgc tgaaaatgag ctacgttgaa ggtctgcttt ccagcaatca
    26701 gaaagatgtg ctgatggaag agatcgtcgc aaactaccac gctaacacca aagacgctga
    26761 agtcgttctg gttgaaggtc tggtcccgac acgtaagcac cagtttgccc agtctctgaa
    26821 ctacgaaatc gctaaaacgc tgaatgcgga aatcgtcttc gttatgtctc agggcactga
    26881 caccccggaa cagctgaaag agcgtatcga actgacccgc aacagcttcg gtggtgccaa
    26941 aaacaccaac atcaccggcg ttatcgttaa caaactgaac gcaccggttg atgaacaggg
    27001 tcgtactcgc ccggatctgt ccgagatttt cgacgactct tccaaagcta aagtaaacaa
    27061 tgttgatccg gcgaagctgc aagaatctag cccgctgccg gttctcggcg ctgtgccgtg
    27121 gagctttgac ctgatcgcga ctcgtgcgat cgatatggct cgccacctga atgcgaccat
    27181 catcaacgaa ggcgacatca atactcgccg cgttaaatcc gtcactttct gcgcacgcag
    27241 cattccgcac atgctggagc acttccgtgc cggttctctg ctggtgactt ccgcagaccg
    27301 tcctgacgtg ctggtggccg cttgcctggc agccatgaac ggcgtagaaa tcggtgccct
    27361 gctgctgact ggcggctacg aaatggacgc gcgcatttct aaactgtgcg aacgtgcttt
    27421 cgctaccggc ctgccggtat ttatggtgaa caccaacacc tggcagacct ctctgagcct
    27481 gcagagcttc aacctggaag ttccggttga cgatcacgaa cgtatcgaga aagttcagga
    27541 atacgttgct aactacatca acgctgactg gatcgaatct ctgactgcca cttctgagcg
    27601 cagccgtcgt ctgtctccgc cagcgttccg ttatcagctg actgaactgg cgcgcaaagc
    27661 gggcaaacgt atcgttctgc cggaaggtga cgaaccgcgt accgttaaag cagccgctat
    27721 ctgtgctgaa cgtggtatcg caacttgcgt actgctgggt aatccggcag agatcaaccg
    27781 tgttgcagcg tctcagggtg tagaactagg tgcaggcatt gaaatcgttg atccagaagt
    27841 ggttcgcgaa agctatgttg gtcgtctggt cgagctgcgt aagaacaaag gcatgaccga
    27901 aaccgttgcc cgcgaacagc tggaagacaa cgtggtgctc ggtacgctga tgctggaaca
    27961 ggatgaagtt gatggtctgg tttccggtgc tgttcacact accgcaaaca ccatccgtcc
    28021 gccgctgcag ctgatcaaaa ctgcaccggg cagctccctg gtatcttccg tgttcttcat
    28081 gctgctgccg gaacaggttt acgtttacgg tgactgtgcg atcaacccgg atccgaccgc
    28141 tgaacagctg gcagaaatcg cgattcagtc cgctgattcc gctgcggcct tcggtatcga
    28201 accgcgcgtt gctatgctct cctactccac cggtacttct ggtgcaggta gcgacgtaga
    28261 aaaagttcgc gaagcaactc gtctggcgca ggaaaaacgt cctgacctga tgatcgacgg
    28321 tccgctgcag tacgacgctg cggtaatggc tgacgttgcg aaatccaaag cgccgaactc
    28381 tccggttgca ggtcgcgcta ccgtgttcat cttcccggat ctgaacaccg gtaacaccac
    28441 ctacaaagcg gtacagcgtt ctgccgacct gatctccatc gggccgatgc tgcagggtat
    28501 gcgcaaaccg gttaacgacc tgtcccgtgg cgcactggtt gacgatatcg tctacaccat
    28561 cgcgctgact gcgattcagt ctgcacagca gcagtaatct cgtcatcatc cacagctttg
    28621 cgctgcggat atctgaaccg gaaataatca ctatttccgg ttttttattc tcttaatttg
    28681 cattaatcct ttctgattat cttgcttaac tgcgctgcat caatgaattg cgccatctca
    28741 ctttgcatac ttaccacttt gttttgtgca agggaatatt tgcgctatgt ccgcaatcac
    28801 tgaatccaaa ccaacaagaa gatgggcaat gcccgatacg ttggtgatta tcttttttgt
    28861 tgctatttta accagtcttg ccacctgggt agttccggtg gggatgtttg acagtcagga
    28921 agtgcagtat caggttgatg gtcaaacaaa aacacgcaaa gtcgtagatc cacactcatt
    28981 tcgcattctg actaacgaag caggcgaacc tgagtatcac cgcgtacagc tgttcacgac
    29041 gggcgatgaa cgcccgggcc tgatgaactt cccgtttgaa gggttaacct caggatcgaa
    29101 atacgggaca gccgttggca tcatcatgtt tatgctggtg attggcggcg cgtttggcat
    29161 tgtgatgcgt acaggaacca ttgataacgg tatcctggcg cttattcgcc atacccgcgg
    29221 gaatgaaatt ctctttattc ctgcgctgtt tattctgttt tcacttggcg gcgcggtatt
    29281 tggtatggga gaagaggccg tcgcctttgc cattatcatc gcaccgctaa tggtccggct
    29341 gggctatgac agtattacca ccgtcctggt gacttatatt gccacgcaaa tcggttttgc
    29401 cagttcgtgg atgaacccgt tttgtgtggt tgttgctcag gggattgccg gcgttccggt
    29461 gctttctggc tccgggttgc gcatcgtggt gtgggttatc gccactctga ttggcctgat
    29521 ctttaccatg gtgtacgcct cacgagtgaa aaagaatcct cttctgtcac gcgtgcatga
    29581 gtccgaccgc ttctttcgtg aaaagcaggc ggatgttgaa caacgtccgt ttacctttgg
    29641 tgactggctg gtattgattg tcctgacagc cgtaatggtc tgggtgattt ggggcgtgat
    29701 cgttaatgcc tggtttattc cagaaattgc cagccagttc ttcaccatgg gtctggtgat
    29761 tggcatcatc ggcgttgttt tccgccttaa cggcatgacg gttaatacca tggcttcatc
    29821 ctttaccgaa ggggcgcgaa tgatgatcgc ccctgccctg ctggtgggtt tcgccaaagg
    29881 gattttgctg ctggttggta atggtgaagc aggtaatgcc agcgtgttaa atactatcct
    29941 caacagcatt gccaatgcca ttagcggtct ggacaacgcg gtcgcggcct ggtttatgtt
    30001 gctcttccag gctgtattta atttcttcgt gacgtccggt tctggtcagg cggcattaac
    30061 tatgccgtta ctggcaccgc ttggcgatct ggtcggtgtt aaccgtcagg ttaccgtgct
    30121 ggcgttccag tttggtgatg gcttcagcca catcatttac ccaacctcag cttcgttaat
    30181 ggcaacgctc ggtgtgtgcc gggtggactt ccgtaactgg ctgaaggtgg gcgcgaccct
    30241 gcttggactg ctgtttatta tgtccagcgt cgtagtgatc ggcgctcagt tgatgggcta
    30301 ccactaaaaa tgttaagagc cgcaattgcg gctctttttt cattctgccg tttcagtctc
    30361 tacggcttca tttttggcat tgcgcttcat ccacaacgcc agcgctttca gcgagtctgg
    30421 agtgaactca tcgcagcgtg cggtgatttc ttccggcgtc agccagcaaa ctccactgac
    30481 ttcatcttcc tgtagggcga agggaccgtg agagacgcaa ctgaacaacg cgccccagac
    30541 acggcaattt ttatcttcga aatagaactg cccgtgctcg gcaaagggga caccggcaat
    30601 gcccaactct tcttccgctt cgcgacgcgc ggattccagc agttgctcat cggcctgaac
    30661 tacaccgcct gcggtcgcat ctaacatgcc gggtaaaaag tcttttgtct cggtacgacg
    30721 ctggaccaga attttgccca tgccatcatg cacgacgatg taagttgcac gatgacgcag
    30781 acactgtgcc cgcatttgtt cccggctggc ttgtgcaatg acttcgttct cttcattgac
    30841 aatatccacc cattcagtac ttgccaaacg acgctgttcc attatcagga aaccttcttt
    30901 ttctggcgct cttacggcgc attttgagtt gtgggtaaat tacggattaa tcgcgacctg
    30961 cgcaatgata ctttgatcat tgagtgcgat tacactaagt acgtcattat ccagcatgcc
    31021 ataacccgcc ggattaccgc ctttcggaat actcaccgag ccggggttga agtggaaaat
    31081 ctccccccgc tgttctgcca ctggcagatg ggtatgaccg tacaccagca catcgttctg
    31141 gtttaaagca ggtagatttt ccgggccaaa aagatgacca tgcgtcaaaa acagacgttg
    31201 tttttccagt aatacctgtt gccacggcgc ggttatcggg aaatgcagca tcatttgatc
    31261 cacttcgctg tcgcagttgc cacgcacagc gataacctta tgtgccactt cattaagccg
    31321 ttcagcgact ttggctggcg cgtaaccctc cggtaaagca ttacgcgggc cgtgattcaa
    31381 cacgtcgcca aggatcacca gccactgggc accgctttgg acaaacaact ccacaacacg
    31441 ttccgtcgcc ggtaacgacc catgaatgtc cgatgcaaac atcagtttca tcactcactc
    31501 ctcgtcgaaa aaacgtaagc tatgatactg gattctgtcg ttgctatcag ccagattgct
    31561 tcgccgaaag tgcaataaaa cgctggacag acgctcgctg ccactggaat gtcgcataat
    31621 ccaccaggcg ttctggcacc tcatcgccat gtagtaccag gcggttaatc attagcgcca
    31681 gatcagtatc agcaatgcac cattcaccaa ataaattcgg ctgacccagt gctaacaaat
    31741 gttctgccat cgcgaacagt ttctctgcac tggcttttcc ctcggccgtt agtggcgctt
    31801 ttttcgcccc cgcaaagaca acatccgtcg gacgctcttc gcggatgggc atcagatcgc
    31861 tgcgcagcca ggcctgaatc tgtcgtgcac gcgcacgatt ttctaaatca agcggataaa
    31921 tacgttccca ggtcggtggc gcaaatcgat cttccagata ctccgcaatg gcagaagatt
    31981 cactcaactc aaaatcatcg atttgtaata acggcacacg gcgtgtctga ccgtaacctt
    32041 gccacgtcgg ctgcaaatgt tcaccgctgt cgaggtcgat ggtcttgata tgaaacgaca
    32101 ggcctttttc ctgcaacgcc acccaggcgg ataacacata aggggagaaa aagtgggcat
    32161 ctgaccaaag cgtgatagcg ggtttactca ttatgtcctc ggcaataaag ggggctggct
    32221 atcaaactat agagcctttt tgtcactgtc acgtgacgaa tactcacgta agtcgccgcg
    32281 ctttctatac ttgtggtgtt cagatgatta aacaaccgga gctgcaatga tcgatctcta
    32341 tttcgccccg acacccaatg gtcacaaaat tacgctgttt ctcgaagaag cagagcttga
    32401 ttatcgcttg attaaggtag acttggggaa aggcggtcag tttcgtccgg aatttttgcg
    32461 catttcgccc aacaacaaaa ttccggcaat tgttgatcat tctcctgccg atggcggcga
    32521 accgttaagc ctctttgagt ctgctgccat tttgttgtat ctggctgaga aaacaggact
    32581 ctttttgagt catgaaacgc gtgagcgcgc cgccacatta cagtggttat tctggcaggt
    32641 aggcggactg gggccgatgc ttgggcaaaa tcatcatttt aatcacgcag ccccccaaac
    32701 cattccttac gctattgaac gttatcaggt tgaaactcag cgtctttacc atgtactaaa
    32761 caagcggctg gaaaactcgc cctggctggg aggcgagaac tacagcattg cggatattgc
    32821 ctgctggccg tgggttaatg cctggactcg cctgcgaatt gacctcgcaa tgtatccggc
    32881 agtcaagaac tggcatgagc ggatccgttc gcgccctgcc accgggcagg cactgctaaa
    32941 agcacaactc ggtgatgagc gttcggatag ttaacagaaa caggttctcg tgtattattt
    33001 catcctaagt aaaacaacgg agaacctgca atggcacaac ctgccgctat tattcgtata
    33061 aagaaccttc gtttgcgtac gtttatcgga attaaggaag aagaaattaa caaccgtcag
    33121 gatattgtta tcaatgtgac gatccactac cccgccgata aagcgcgcac tagcgaagat
    33181 atcaacgatg cgctgaatta tcgcaccgta acgaaaaaca ttattcagca tgtagagaat
    33241 aaccgtttct ctttgctgga aaaattaact caggatgtgc tcgatatcgc acgtgaacat
    33301 cactgggtga cgtatgctga agtggagatc gataaactgc acgcgctgcg ctacgccgat
    33361 tcggtatcca tgaccttaag ctggcagcgt taatcgccat atcgggaggc tgcatgaaca
    33421 tagtgatcac cggagggacg ggattaattg gtcgccattt gattccgcgt ttgctggagc
    33481 tgggccatca aattacggta gtgacgcgta acccgcagaa agccagttcc gttctcggcc
    33541 ctcgggtgac actatggcaa gggcttgccg atcaaagcaa cctcaacggc gttgatgcgg
    33601 taatcaacct ggccggagaa ccgattgctg ataaacgctg gactcacaag caaaaagagc
    33661 gtctctgcca aagccgctgg aatatcacgc aaaaactggt cgatttgatt aatgctagcg
    33721 acacgccgcc gtcggtactc atttccggct cggcaacggg ctattatggc gacttaggtg
    33781 aagtggtggt taccgaagag gaaccgccgc ataacgaatt tactcataaa ctctgcgccc
    33841 gctgggaaga gattgcctgc cgggcgcaaa gtgacaaaac gcgagtgtgc ctgctgcgaa
    33901 ccggcgtggt actggcaccg gacggcggta ttctcggtaa aatgctgcca ccgtttcgtc
    33961 ttggcctggg cgggccgatt ggttccggtc ggcagtatct ggcctggatt catattgatg
    34021 atatggtcaa cggcattctc tggctgctgg ataacgagct gcgcgggcca tttaatatgg
    34081 tttcgcccta cccggtacgc aatgaacaat ttgcccatgc gctcggtcat gcgctgcatc
    34141 gcccggctat tttgcgcgtc cccacaaccg ccattcggct gttaatgggc gaatcttcag
    34201 tactggtatt aggtggacaa cgcgcgctgc ctaaacggct ggaagaagcg ggttttgcgt
    34261 ttcgctggta cgatttagaa gaggcgctgg cggatgtcgt tcgctgatgt ggtttacagc
    34321 aaacatccgc cagttaactc cctgtgttac aggattagtg gctttgcgcg ataagatcgt
    34381 ctggtgaaag tcgggtcacc atcataacta actctctgtc taaacctcta tccagcatct
    34441 cctgagcaat acgcagggct tcttcgtgtt tgccctgcat tgcgccttct tcacgtaatc
    34501 tgtcagcaat ggtcatcagt ttctcctttt cttgtggtgc gcgttctgtt atctcaccaa
    34561 taaatgcacg gaaacgctgg gcatcccctg tttgtaatac gtaattaaac agggctttta
    34621 gctgtctgtc attagtgttc cctgtaacta gcagcgaaac aatttggtcg actaatccca
    34681 acagatcgcg ctgacgaata tgtttctgaa ttaactccaa cagcgccatt ttgcggtgtt
    34741 gcataatctc gtcatccggc accacggtaa tatccaccaa cggaaacgct gatgaatata
    34801 ttttgcgggc tatagcaggc tcggcaaatt catcaagcca gcagagtgaa taaggataag
    34861 gacttctgca accatgataa aacagcatcg ggatcaccaa tggaagctct ttatagcccg
    34921 catcaagatg gttttgcatt gccgcaatgg aataacgcat catgcgaaaa gccattaatt
    34981 cttccggctt actttggtgc tctatcacta cataaatata acccactccc tcctgcgttt
    35041 tcacagacca caagaggtcg gaataatatt gccgcaggtc ttcatcaata aaactgtttg
    35101 gttctagttt aagcgtcgtt aaatcacaca gtttgcgcag cggcgcggga agatgaatat
    35161 taataaaatt ccgcgcggtg tctggatggc gtaaaaaaga tttaaatacc gcatcatgcg
    35221 gcgtggaagt tgtcgatatt gtcatggcgt tccgtcaccc ctcaaattta agatgacgcg
    35281 acaataacca cgattaacac ccaatgcagc aggcagtttt tatctttgga cagcgaattc
    35341 agaagtattt ttagcggact gcaactttta catttcagcg gaagctgact cgcaaattcc
    35401 ccttgaaaat actccggcag cgttagccac cggagaggaa attatttcag cgatccctta
    35461 aggaactgtt gcagacgagg gctttgcggg ttgccaaata actgctccgg cgcgccctct
    35521 tcttctattt tcccctgatg gaggaaaatg acgtgagtag aaacatggcg agcaaagccc
    35581 atttcgtgag tcactaccac catggttttc ccctcttctg ccagttgctg cataatacgt
    35641 aacacttcgc ctaccagttc aggatcgagc gccgaggtag gttcatcaaa cagtaaaact
    35701 tctggttcca tcgccagcgc ccgcgcgata gaaacacgct gttgctgacc gccggaaaga
    35761 tgcaccggat atttcccctg cgcacgttcg tctatcccga ctttcgccag atacttcacc
    35821 gcccgctcgc gcgcttcctg cttgctcagg cccaacacct gaatcggcgc ttccatgacg
    35881 ttttccaaca ccgtcatatg gctccagaga ttgaagtgct ggaataccat cgtcaggcgt
    35941 gtgcgcagta agcgcagttg atttttatcg gcgactttga gttgaccgtc tttgtcgcgc
    36001 accagattga tcgtctggcc attgaccacg atcgatcctt cactcggttt ttcgaggaag
    36061 ttaatgcagc gcagaaaggt acttttcccc gatcccgacg atccgatgat gcttattaca
    36121 tctccggcat tcgcttgcag tgagacccct ttcagcactt cgtgttcgcc atagcgtttg
    36181 tgcaaatcga taacgtttaa tttattctcg gacatcgtat tctcagtgcg ttgaagaagg
    36241 tttcacatgc tgcaaccagc gtttttccgc tctgcgaaag aggctgatca ggacataaga
    36301 gatgattaaa tagagcaccg cggcaatgcc gaaggcggta aaaggttgat acgtggcggc
    36361 gttaatatcg cgggctattt tcagcagatc cggcacagtg gccgtaaacg ccagcgcagt
    36421 agagtgcagc atcaggatca cttcgttgct gtatgccggt aacgcaatac gcagcgcaga
    36481 aggcaaaata atgcagcgat acattttaaa ggtcgagaag ccataggctc tggcggcttc
    36541 aatttcccca tgcggaaccg aacggattgc cccagcaaaa atctcggtag tgtaagcgca
    36601 ggtgttaagc gtcagcgcca gcacggtaca gttcaggcca ctgcggaaga aagcgttaag
    36661 gaattcggtt cccttaacaa tctcaagcgt gtacatgccg gaatagaaca ccagcaactg
    36721 aacatacagc ggcgtaccgc gaaaaatata ggtaaataac cagattggaa actggatgta
    36781 tttattactg gagacacgac caatcgccag aaacagcgcc aggactccgc ctatcactac
    36841 cgacaaaata agcagccaca gagtgatcgc cacaccagta aagcgataac cgtcggtcca
    36901 cagcagcggt ttccagtatt catgtaagat ttcgatcaca ggtcagccct cttcacaccc
    36961 acggagtagc ggcgctcaag gaacagcagc acaccattgg aaacggtggt gaaaaccagg
    37021 taaatcacgc cacagacgat ggcgaaatag aacggttccc aggtactttt gcctgccagc
    37081 tgcgtggctt tgaccacatc ttccaggccg agtaacgaaa ccagtgcggt agatttgagg
    37141 atcacctgcc agttgttgcc aatgcctggc agggcgtaac gcatcatcgc cggaaacatg
    37201 atccgccgaa acacttgccc gcgagtaaaa ccgaacgccg tcgccgcctc tatatgccct
    37261 ttcggcactg ccataaacgc gccacggaac gtttcggtaa aataagcacc gtagataaaa
    37321 ccgagagtga taataccagc gaccatcgga tcgatatcaa tctgcccgac gcccatcgcc
    37381 tccgtcacca tgtttagcgc aatctgcaaa ccatagaaaa tcagcagcat caacaccaag
    37441 tccggcacgc ctcgaatcag cgtggtgtac ccttcgaaaa tcagccccga aagccgattt
    37501 tgcgagagct taccgccagc gccaattaaa ccgatgatta cagcgagcac tacagagctg
    37561 atagccagct ccagcgtgac gagcgcaccc tgtaaaataa cacctgaaaa cccatacaac
    37621 atgctgcctg tcctgtcgtg tgtggtgaat taccgcctct tgtctccctc cggttacccg
    37681 aagggagcgg gctttctttc accgatggcg attaaccacc ataaacatca aaatcgaagt
    37741 actttttcgc taatttctcg taagtaccgt cagcgcgcat ttcggcaaag gctttgttca
    37801 gtgcctcgcg cagttcgtta tcttctttac gcaggcccat gccggtccct acgccaaaca
    37861 gtttttcatc tttaacagac ggaccaccga atttgtaatc tttaccgacg ggttgtttga
    37921 ggaaaccttc gctggcagcg acctcatcct ggaacgcggc atcaatacgt ccggcagtca
    37981 ggtcagaata aatgttgtcc tgcccctgat acgagacgat ttcaatgcct tttggtgccc
    38041 agtgttcatt accgaacgtc tcctgggtgg tgccctgcaa tacgcctacc cgtttgcctt
    38101 tcagcgactc gactgtcggc tgaatgtcag aatttttcgc caccaccaga cgagaatcgg
    38161 cagcgtacag tttgtcggtg aaggcaattt cttgctgacg tttttccgta atggaaagcg
    38221 atgacatgat ggcgtcaatc ttcttcgctt ttaaggacgg gattaacgca tccagcgggt
    38281 tttcgacaaa cgtacattgc gtattgatgc gcttgcacaa ctccttcgcc agctcgatat
    38341 cgaaaccaac cagttcgcct tgtgaatttt ttgattcaaa tggcgcatag gtcgggtcgg
    38401 taccgatgcg gatgttttgc ggaattgcag caagtgccgc agttgcgctg gagaaggcca
    38461 gaaccagaga gagcgatagc accagttttt tcataatgtc ctcaactgac agtcttttta
    38521 tagggaatat ttacaggttt gttacactta tcgtgccata aaattgacca tcaaggcaaa
    38581 atatcccgcc ccgttagacg tattattttt cattaaaaat gcttaagtat gcatttcaat
    38641 gcaccacgat agtgcaacaa acctggcgtg caccatgatg atcattccat caggtacagc
    38701 ttcccagcga cgtatcagtc accgtagaca ttaaagtcga aatacttttt cgccatcttg
    38761 tcgtaggtgc cgtcctgacg cagctcgcca agcgccttat tgaaggcagc cgtcagttca
    38821 gcataatctt tacgtagccc tacaccggtg ccatcaccga agtatttttt gtcttttact
    38881 gatgagccag caaaagcgaa atctttacca gcaggttgct tgaggaatcc ttcgctggca
    38941 gcaacttcat cttgtaacgc agcatccaga cgtcctgcag ccagatcgga atagaccaaa
    39001 tcctggttgg cataggccac cacatccacg cctttactac gccaggtctc gttagcgtaa
    39061 gcttcctggg ttgatccctg cagcacacca acatgtttac ctttcagtga atccagcgtt
    39121 ggctgaatcg gtgaaccttt ggcagcaatc aaacgagaat ctgcggcgta cagcttgtcg
    39181 gagaaggcaa tctcctgctg acgtttatcg gtaatggaaa gcgacgaaat aatagcgtcg
    39241 atttttttcg ctttcagtga ggggatcagc gcgtcaaagt cactggcaac ccaggtacat
    39301 ttcacctgca tccgtttgca catctcgtta ccgagatcga tatcaaagcc aacaaaatca
    39361 cctttagcat ctttcgacga gaacagcgca taggtggtat cggttccgat acgtaccgtc
    39421 tccggtagcg ccgcatagct ggaagccgct gcggagagac cgactaataa agacagagcg
    39481 agaatcgact tcttcataca taaccctcaa gtgaaaatgg cttttttatg ttgtgtattg
    39541 tgttgtgtgt ttgcaggctc tttcatgcag gtcttatgcc atcttgacga caacagtaac
    39601 attcaacgtt aaatatgtta ataagacatt gcattattgt cctgaagttg aagatagcag
    39661 gtatagcggt tgaatcgcag cgtttcgatt gaatggcaga aacaaaatgt cgaggatttg
    39721 atcgcggttg caaaattgcc ctgaaacagg gcaacagcgg agttatgcgc cctgccagcg
    39781 ggcaaagaga tcttcaggaa gggttattgc aaactggtca agaacacgat taaccgtctg
    39841 atttatcaca tcatcaaggg attgcgggcg atgataaaac gccggaacgg gaggcataat
    39901 caccgcaccg atttctgccg cctgagtcat taaacgcaga tggcctaagt gcaatggtgt
    39961 ttcacgcacg cagagcacca acgggcgacg ctctttcagc accacatctg ccgcacgggt
    40021 cagtaagcca tcagtatagc tatggacaat gccggacagg gttttgattg aacagggtaa
    40081 aatcaccatc cccagcgtct ggaaagaacc ggaagagatg ctggcggaaa tatcgcgcgc
    40141 atcgagcgtg acatcagcta atgcctgcac ttcgcgcagg gaaaaatccg tttcgaggga
    40201 taaggtctgg cgcgccgcct ggctcatcac cagatgcgtt tcgatatctg tgacatcgcg
    40261 cagaacctgt aataagcgca cgccataaat cgcgccgctg gcaccgctga tgcctacaat
    40321 gagtcgtttc ataaatagtt gccctgtcag acttgcaggc agactttgca gtatttcggc
    40381 gggagttgca agtcagggtg ccagaccggc accctcagcg aaggcatcat ccttcgttat
    40441 gcatttcgag attttccact tcgttctgac gttgcactgc tttggcgtca tcattacgta
    40501 acgtatcgag gaaatcgagg tagccctgat caacatcttt ggtgacgtag acgccgttga
    40561 acaccgagca ttcaaactgc tggatatccg gattttcagc gcgaacggcg tcgatcagat
    40621 cgttcagatc ctggaaaatc aacccgtcag caccgatgat ctggcgaatt tcatcaactt
    40681 cgcgaccgtg agcgatcagt tccgtggcgc tcggcatatc aataccataa acgttcggga
    40741 agcgaatttc cggtgccgca gaagcgaggt acactttctt cgctccggct tcgcgtgcca
    40801 tctcgataat ctgctcagaa gtggtgccac ggacgatgga gtcgtcgacc agcaggacgt
    40861 ttttatcgcg gaactcggcg cggttggcat tcagtttacg gcgcacggac ttacgacgca
    40921 gctgctggcc cggcatgata aaggtgcggc caacatagcg gtttttaacg aagccctggc
    40981 ggtacggttt gcccagaata cgagcaattt ccagcgcgat atcacacgag gtttctggga
    41041 tcgggatcac cacgtcgata tccagatctt cccattcgcg ggcaattttc tcgcccagtt
    41101 tcgtgcccat attcacacgc gcgctgtaaa cggaaatttt gtcgataaac gagtccgggc
    41161 gggcaaagta tacatactca aacaggcacg gattgctgac cggattgtca gcacattgac
    41221 gggtaaacaa ctgcccttct tcagtgatgt aaatcgcttc gcccggcgcg acgtcacgca
    41281 ggaaatcaaa gcccagcgta tcgagcgcta cgctttcgga agcgaccata tattctgtac
    41341 ggttctcgtc aatatcacgt tttcccagta ccagcggacg aatcccgttt ggatcgcgga
    41401 aagcaaccat accgtggccg ataatcatcg ccacacaggc atacgcgccg cggattaagc
    41461 ggtttgtggc agcaatggca gcgaaaatat tgtcggcttc cagcgggtag tggcggaagt
    41521 tgtccagctc gctggcgaag atattaagca gaatttccga gtcggaagtg gtgttgatgt
    41581 ggcggcgttt ttcttcaaac agttttttac gcaactcgtg agcgttggtc agattgccgt
    41641 tgtgggcaag cgtaatgcca tacggggagt taacgtaaaa cggctgcgct tcagaggcgc
    41701 tggagctgcc agccgtaggg taacgcacat gaccaatgcc catattgccc tgcaaacgct
    41761 gcatatggcg agcttcaaat acatcgctca ccagcccgtt cgctttacgc aaacggaagc
    41821 agttattggc atctatggtg atgatgccgg cggcatcctg accgcgatgc tgaagcaccg
    41881 ttaaggcatc ataaatcgac tggttaaccg gcataacacc ggcgataccg acaataccgc
    41941 acatacgtct ttttcctcgt taagccacat ctcagagcac ttacgctctg ggcaagaaac
    42001 ttgacgagct ttgcagataa tcaaaaaacc atctgatgat aaaactgaat tgcgggatca
    42061 gctgtgattt gctccagtct tcacttttcg acaccccggt aaaggagtcg agaaagaaga
    42121 gaatggcggc aacaatcaac acaccgcgca acgcaccgaa acagacgccc agcacccgat
    42181 cggtgcctga caaccccgtt ttctccacca actggcctat cacgaagttc acgatagcac
    42241 caacgatcag ggtagcgata aacagtaccg cgatggcaat cccatttcga accagttcgt
    42301 cttcaaagcc cgtaaaccag actgacaggt aagtgtagta atgactggca acaaagaaag
    42361 cacaacccca tgtcaccagc gataacgctt cacgaacaaa gccgtggatc aggctaacca
    42421 gagaggaaaa agcaatcacc gcgattatgg cgtaatcaat ccagaccata tgtgtcccac
    42481 gattttacgc cctgtcatcc tgttcggggc gcattctaac agaaaaagaa aacgtttgcg
    42541 tagggatttc cttcccgcgc atcaataaaa atggcgctga aaaaatattc aacgccatcg
    42601 actttttatg cctttgcggc atcgtgcaat gcgtgtcgga tgcggcgtaa acgccttatc
    42661 cgacctacgg ttctaacact gcccaagcct gataagacgc gccagcgtcg catcaggcaa
    42721 gactgtatta attcggcgta tagcccatta ccacgccact taagccagaa agttgcttca
    42781 actcacccag cgaacctttc agcttatctt tcgaggcatc cggcccaacc agaatacggg
    42841 taattttacc ctgcactggc gtggatggcg acgtataaac ccgataaccg gcaccgcgca
    42901 gcttaccgac aatctcattc actttatcgg catttttcag cgcacccagt tgcacaacat
    42961 aggctttacc cgttggcgct gctttttctt ccacgaccgg cttcggttct ggcttcggcg
    43021 caggtggtac ttcaaccttt ggtttaggcg gctccaccgg tttcggcttc ggtggggcga
    43081 ccggtgcagg ttccggttca aactcggtgt tattggctgc aatagtggcc ggatcgagcg
    43141 acggtgctgc ggcatcacct gcccgcacct cttccgctgc gccttccggc ggctgcgtcg
    43201 gcaacgcctg ggtggcggct ggcatcatat caggctcatc acgatcgccc gctttcggca
    43261 ccagcgggat agccgcgaac tcatcctgat aatgtttttt ctgcccgtcc agcagccctg
    43321 gaagtacaat cacccccagc gccaccagca cgatcgtgcc cactaaccga ttctgaaact
    43381 tacttgccac cgcttctcct cgcgtcaatc acttccatga catgtgcgac cgtgtggaaa
    43441 gaaccacaca ccagcacggt gtcttccgct ttagcgtccg ccattgcggc atcccatgcc
    43501 tgcgcaacgc tatcaaatga tttgccgtta cccaaatgct caagcagttg ttctgccgtg
    43561 gcaccgcgcg gcccttccag tggcgcacaa taccagtcat caaccacgct tttcaaccag
    43621 gccagagttc cggcaatatc tttatcatgt agcataccga taaccgccag cacgcgcccg
    43681 tttttcggta gcgctttcat acgcccggtg agatattccg ccgcatgtgg attatgcgcg
    43741 acatcaaaaa taacgcgtgg cgactcgctc acaatctgga aacgtcccgg caaaattgcg
    43801 ctggcaatcc cgtcgcgaat ggcattttca ctgacttcca gcccgctggc acgcagtgcc
    43861 gccagcgccg ttgcggcatt cggttgcggg acaagcggca acggcagatt ttccagcgtg
    43921 ccgtgagcat cgctaaacgc ccagtcatga tcggtgacgg aatagttcca ctcaacgccc
    43981 cgacgttgta acagtgcacc tttttcctgc gccacatcag caatggtaga aggcatttcc
    44041 ggctcaccga caattgccgg tttttcgctg cggaagatgc ctgctttctc gagaccaata
    44101 ctttcgcgat ctggacccag ccagtcggta tgatccagcg caatactggt tactaccgcg
    44161 acatcggcat cgacaatatt ggttgcgtcc agacgaccgc ccagccctac ttccagaatc
    44221 accacgtcaa gttgtgcctg cttgaacagc cacaacgccg acagcgtacc gtactcgaaa
    44281 taggtcaggg aaatatcacc gcgtgccgat tcaatctccg caaaagaggc ggtgtgggcc
    44341 gattccggca attcctggcc ctgcacacgt acgcgctcgg tataacgcac cagatgaggc
    44401 gaactgtaga cgcccacttt gtaccctgcc gccatcagaa tcgactccag cgtacggcag
    44461 gtggtgcctt tgccattcgt acccgcaacg gtaaacacaa atggcgctgg tttcaggacg
    44521 ccaagacgcg ccgcgacctg gctcacgcgc tcaaggccga gatcgatagt tttactgtgc
    44581 aggttttcca gataagaaag ccacgaagcc agaggcgacg cggcttgagg agtgcgtttg
    44641 ataatcatgg tatccgctga ttcgttacgg tgagaatagc aaaagggcag agccaatggc
    44701 cctgccctta tcagttatca ggcctcaggt tcctgatccg gtaccggggg taccactacg
    44761 ccttcacgcg gcgcttcagg attcggcgct ggcagattca tcaacttcgc cagaatgctc
    44821 gccagtttca ggcgcatttc cggacgacgg acgatcatgt cgatcgcgcc tttctcgatc
    44881 aggaattcac tgcgctggaa tctaggcggc agtttttcgc gaacggtctg ttcgataaca
    44941 cgcggaccgg caaagccgat taacgcttta ggttcagcga tgttgagatc gcccagcatg
    45001 gcgaaacttg cagaaacacc gcccatcgtc gggtcggtca gcacggagat gtacggcaag
    45061 ccgcgctcct gcatttttgc cagtgccgca gaggttttcg ccatctgcat cagcgacatc
    45121 agtgcttcct gcatacgtgc gccaccagag gcggagaagc agatcagcgg gcagttatct
    45181 tcctgcgcct gctcaacggc acgcacgaaa cgtgcaccca caacagaccc cattgaaccg
    45241 cccataaagg cgaactcgaa tgccgcagcg acaaccggca ttccatacag agtgcctttc
    45301 atcaccacca gcgcatcttt ttcgccggtt tctttctgcg cagatgccag acggtcttta
    45361 tacttcttgg agtcacgaaa cttcagctca tctttcggct caagctcgct acccagctcc
    45421 acaaggcttc cttcatctaa caggctatgc aggcgattac gcgctgtcat acgcatgtga
    45481 tggtcacact tcggacagac ctcaagatta cgttccagct cagcgcggta taaaacctga
    45541 ccgcagctat cacacttagt ccacacccct tcaggaatgc tcgccttgcg ggtgggagta
    45601 atgttgcttt taattcgttc aatccagctc attagggacc tttctgtctg aacctggttc
    45661 gatgccagtt ttatctttgg ggacgcataa tgccattttt gcccccaaca gaccatgaat
    45721 gttgcacatt aaaacataac agcccgaaac tttggataaa aaagtggtcg aaccgcggag
    45781 ttacttttta ttttgcggcg cgtgccgcag cgcgtttgtg acggattatt tcgatgacgc
    45841 ccggcaaaat agaaaccaca ataatcccga cgatcagcag cttaaggtta tcctgaacca
    45901 tcgggattgt accgaagaaa tagcctgcgt aggtaaaaag cagtacccac aacagtgcgc
    45961 cgatcacgtt ataagcagcg aaatgacggt acgacatgtg gcccattccc gcaacaaacg
    46021 gggcgaacgt tctgacgacc ggcacaaaac gggcgagaat aatcgttttg ccgccatgtt
    46081 tctcataaaa ctgatgggtt ttgtcgagat aactgcgacg gaaaattttc gaattgggat
    46141 tactgaataa cttttcaccg aacaaccgcc cgatagtgta attgaccgcg tcacccacaa
    46201 tcgcggcgat cagcatcagc actaccatca tatggacgtt gagatcgttg gtttccagtg
    46261 atgccaacgc tccggcaaca aacagcaatg aatcacccgg taaaaacggc attaccacca
    46321 gaccggtttc acagaacaaa attaagaaca aaatggcata aacccagacg ccgtactccg
    46381 cgactagctc tgccagatgc acgtcaatgt gcaggataaa atcgatgagg aaataaatca
    46441 ggtccatatt tgcctatgcc ttgtactcgt catctttcag gctgtaacta cgttggctac
    46501 agcctgaaat gttccgagca ttattctcgt tagtccgcca gaaatagcgg gcccattggc
    46561 ggttttggaa gatcataccg gtcagggtaa tccaccgcga ccagatacag cccttccgct
    46621 tttgccgttg ctgccgccag cgttctgtcc tttgccgcca gcaactctgc tatccagctc
    46681 tccggctggt tgtgggcacc gacttccatc aggctgccga caatattcct gaccatatga
    46741 tgtacaaagg cattcgcttt gatatcaacc accacataag gaccgtgacg cgtgacatta
    46801 atatgcataa cgttgcgcca cggggttcgg gactggcact gcaccgcacg gaacgaggtg
    46861 aaatcattct cgcccagcaa gcattgcgca gcccgatgca tccgttcagc gtccagcggt
    46921 tcgtaaaaat gggttacccc tttactcagt accgccgggc gcagccgatg attgtagatg
    46981 atgtagcgat aacggcgagc cgtggcgcta aatcgggcat gaaaatcatc aggtacagct
    47041 ttaacccaac gcacggcgat gtcaccaggt aaattcgcat ttacgcccaa ggtccacgcc
    47101 gcgtctttgc gctgcgcggt tgtttcgaaa tgcaccactt gcccggtacc gtgtacccct
    47161 gcgtcagtac gcccggcgca gaagacggtg atgggttcgt tcgccacctg ggagagcgcc
    47221 ttttccagct tctcctgcac actgcggact tcattctgcc gttgccagcc gtaatactta
    47281 ctgccgtcgt actcaatgcc cagcgcaatt ttataaactg gcagttgttg ctggtcggac
    47341 attagtacag atactcctgc accagtttct cggcgatttt tactgccatc agcgcgccgc
    47401 caaagcgaac gttatcggcc accgaccaga actggacttg ctccggcata ccgtagtcat
    47461 tacgcacgca gccaacagaa agatgcggcg tacccgaagc atcccctacc tgagtcggga
    47521 attcgttctc ttcagagagc acaatatctt cgccctgagc aaacgcatca cgcgcttctt
    47581 ctgccgccag cggacgcagt gcttcaaagt tgaccatctg ggcatgaccg tagaataccg
    47641 gtgcctggac gacgcttgcc gaaatcatca gcccttcgtc ctgcaggatt ttgcgtactt
    47701 cgtcaacgat acgacgttct tcacgcacgc taccttcgct atccggcaac aacggcagca
    47761 tattgaacgc cagttgacga ccgaagaaat cttcttcgtc aatcggaatg ccgttgagca
    47821 atttcgcact ctgccccgct aacgcatcga ccgctttttt gccctgggcg gaggctgaaa
    47881 tcaggctggt aacactgata cgcgacaaac cgccctgatc gattaacggt ttcagtgccg
    47941 ccagcagctg gctggtcaga ctgtctggta cggcgatgac attccggttg cggtaatcgg
    48001 tcagtacaaa cgggtttact tccggcacca ccagcggtac gtcgggttcg agagcaaaca
    48061 gtccgctgct gtcgatcacc aggcaacctg agttggtcgc ttcttcaacc caggcagcgg
    48121 tagcttcttt gcctgcgaca aaaaatgcca gctgcgcctg cgtccagtcg aattcagcgg
    48181 catcctgcac ggtgattgtt ttaccaccaa agcgcagttg ttcgcctgcg ctttcgttac
    48241 gtgccagtgc ataaatttcc ccaaccggga actgacgttc agccagcgtt tcaagcaggg
    48301 cttcgcccat agcgccagtt gcgcccagga cggcaatgtt ccagccttca gacatggtgg
    48361 tttactccag aaatagcaaa gctccctgcc aatgttacag cagagagcat gaagaagaga
    48421 ttaacgtgcc ggatgatgaa cggcgttaaa acccagttta cacagcaatg atgccgcact
    48481 ggcgtcatca caaattacat acagagatga ccattcacgg cgctcaagat agtttttgcg
    48541 cagtttatcg aactcacccg gtatcccggc gactttacgc agcggtgcgt catcgcggcg
    48601 cacatcatac accaaatgca ccagcctttt cagcgtcggt tgatcgagcg gaccatgcag
    48661 cgtaatgcgg ccaaactcag gcgcgggcag caacgtatcc agcgcaacgt gttgttcatg
    48721 cccaataaac ttgctatagg cttcaaacac ttgcgtcgtg ccacgcgctt taccctccag
    48781 ggtatagcct gcgatatgcg gcgtgccgat atccactttt gtcagcaatt ccacattaag
    48841 ttccggctcg ccttcccaga catccagcac cacgcttaac ttctggcctt cattcaggca
    48901 ggtcagcagc gcggtattat cgacgactgc gccacggcag gcgttaatca gaatcgctcc
    48961 gggcttcagg ctacggatca gtttttcatc cgccagatgt agcgttttgt acggaccatc
    49021 tttaaagagt ggcgtatgga aagtcagaat atccgcgcgc tggactaact catccagcga
    49081 gcggaaatca ccctcatccc cacggtcggc gcgaggcgga tcgcaaagta aggttttaat
    49141 ccctaacgct tccagtcgcg cctgcaatcg acgtccaacg ttaccaacgc ccacgatccc
    49201 caccgtacgg tcgtgcagtg aaaatccatc gcgttcggca agcatcagca gggaggagaa
    49261 aacatattcc accaccgcaa tcgcattaca gccaggtgca gcggaaaaac caattcccgc
    49321 ctgcttcaac catgcttcat cgacatggtc ggtccccgca gtggcagttc caacaaattt
    49381 aatgggtttt cctgccagca aagattcatt cactttcgtg accgaacgca ccatcagcgc
    49441 atccgcgtct gccagttgag cgacggggat tggacgcccg ggaaccgcga tcacctcacc
    49501 caaacggcta aataagtcgc gggcataagg catattttca tcaacaagga ttttcacgtt
    49561 tgtgttacct gtatgagacg agagttaacc ggacaagtat gccataatct cgcggccagg
    49621 catacttgcg aagatttcag gtataaggat acgtaatgat acaacctatt tccggccctc
    49681 ctcctgggca gccaccaggt cagggagata acctgccgtc tggcgcgggc aatcagcctt
    49741 tatccagtca gcaacgtact tcgctggaaa gcttaatgac gaaagtgacc tcactgacgc
    49801 aacaacaaag agcagaactg tgggcgggta tcaggcacga tattggtctg tcgggagatt
    49861 caccgctgct ttcgcgtcac ttccctgccg ctgagcataa tctggcgcaa cgtctgctgg
    49921 ccgcgcaaaa aagccattct gcccgccagc ttttagcgca attaggggag tatttacgtc
    49981 tggggaatca tcgtcaggcg gtcacggatt atatccgtca taactttggt cagacgccgc
    50041 tgaatcagct ctcaccggag caattaaaaa ccattctcac cctgttgcag gaagggaaga
    50101 tggttattcc gcaaccacag cagcgcgagg cgaccgaccg tcctttatta ccggcggagc
    50161 acaatgcgct aaaacagctg gtgaccaaac ttgcggcggc aacgggggaa cccagcaaac
    50221 agatctggca atcgatgctg gaactttccg gggtgaaaga tggcgagtta attccagcga
    50281 aactgtttaa ccatctggtg acctggctac aggcgcgtca gacgctaagc cagcaaaata
    50341 cgccgacgct ggaatcacta cagatggcgc taaaacaacc tttagatgcc agtgaactgg
    50401 cggcgttatc ggcatatatc cagcaaaaat atggcctttc tgcgcaatca tcgctttctt
    50461 ctgcccaggc cgaggatatt cttaatcagc tttatcaacg gcgggttaaa gggattgatc
    50521 cgcgtgatat gcaaccgctg cttaatcctt ttccaccgat gatggacacg ttgcaaaata
    50581 tggcaacgcg tcccgcgctg tggatactgt tagtcgcgat tatcctgatg ctggtctggc
    50641 tggttcgtta accccgacga aatgacagta tcgtgacaat aatacccagc accgcagaga
    50701 tcgccccggc aagaaatacc gaagagtaac caaacgtggt cgccagcatt cccgccagcg
    50761 gcccggagac gccgagggcg atatcctgaa acgcggcgta accgcccagt gcggtgccgc
    50821 gaacttgtga ggggacgcgt ttaaccacct ccacgcccag cgcaggaaag ataagcgaac
    50881 atccggctcc ggttaacgcc gcgcccgcta atgcgaccca tgcacctggg gcttgccaga
    50941 gcagcaacaa gcccaccgtt tctacaagca gagagacaat cgccactttc acgccgccaa
    51001 aacggtccgg catccagcca aacatgacgc gcatcacgac aaatgcgccg ccaaacgcgg
    51061 taagagtaaa gcccgccatc gcccatcctt tgctggcaaa gtagagcgaa acgaaagtcc
    51121 cgataaccgc aaaaccaacg ccttgtagtg ccagccctaa ccctggtttc cagataagcc
    51181 cgacaacgct ccacagcgat ggacgttctc ccgccagggc cggtactttg cgcactgtgc
    51241 cgttacaggc ccacgccagt aagggtaata ccattgtggt gatcgccagt gcggcaaaac
    51301 cgtaatggct atgaatcaac aggccaagcg gcgcaccaac agcgagggca ccgtaaatcg
    51361 ccattccgtt ccatgacatc actttgccag agtgttttgg ccctacgatg cctaaccccc
    51421 aggtcagagc gcctgtcagt aactggcttt caccaaaccc aagaatcaaa cgcccgatga
    51481 ccaacagggc aaatttgaac ggtgcggaga caggcaaaat cgccgccagc agcaacgcgc
    51541 cgccagccag accacaagct aacatcccct gaagcgccga acgttttgcg ccatattgat
    51601 cggccagtcg cccggcgtaa ccacgcgtca gcaccgtagc cagaaactga atcccgacgg
    51661 caatgccgac catggtattg ccatagccca gatcatgatg aacaaacagc gggataaccg
    51721 gcaacggcaa ccctacggtc atgtaggtga gaaaaaccgc aaaagagatg cggaagagcg
    51781 aaaaactggc agaagatcgt gtttcggttt ggcttacagc agtcatgcat tactccagaa
    51841 tgcagcgcaa ggcgaggagt atccccgtct catctctctg gtttcagggt tacggtgcgt
    51901 tggcaggatt taacgcgtac gtcttttcag aaggaaatcg acaaagcggg aagtttgcct
    51961 agaactggtg gcgattgtca atgatgtgaa aaagggaacc atcaggttcc cttttgcgtt
    52021 agtgccggag gccgcattgg cgcgtaacgt cggatgcgac gctggcgcgt cttatccgac
    52081 ctactccggc ctactgcgaa ttaatctttt cagcttgcgc attaccagcg tggcgttggt
    52141 gccgccgaag ccgaagctgt tagacataac agtggtcagt tcgcgatcgg tcgtttcggt
    52201 cacgatgttc agacccgcag cctgctcgtc cagctcttca atgttgatgc tcggggcgat
    52261 aaagccgtgt tccagcatca gcagagagta gatagcttcc tgcacacccg cagcgcccag
    52321 tgagtgaccg gtcatggctt tggttgcaga aatcgccggg ctcttatcgc cgaacacttc
    52381 acggatagct gccagctctt tcacgtcgcc aaccggagtc gaagtaccgt gagagttcag
    52441 gtaatcgatt ggggtatcaa cgccatgcat cgccatctgc atacaacgta ctgcgccttc
    52501 gccagacgga gcaaccatgt ctgcaccatc agaggttgcg ccgtagccaa cgatttcagc
    52561 atagatgtaa gcaccacgcg ccagcgcgtg ttccagctct tcaaccacta ccataccgcc
    52621 gccgccagcg ataacgaaac cgtcacggtg agcgtcgtaa gtacgggagg ctttttccgg
    52681 ggtgtcgttg tatttagtag atagcgcacc catcgcgtcg aattcgcagg ccatttccca
    52741 gcacagctct tcgccgccgc cagcaaacac gatgtcctgt ttgcccagtt ggatctgctc
    52801 tactgcgtta ccgatacagt gtgcggaagt cgcacacgcg gagctgatgg agtagttaac
    52861 gccatgaatt ttaaacggcg tggcgagaca ggcagaaacg ccggatgcca tcgctttagt
    52921 gaccacatac gggccaaccg ctttcaggcc gcgcgggccg cgcattgcgt cagcgccgaa
    52981 cacctggaaa cgcggggaac cgccgccgga acctgcaatc aggccaacgc gcgggttatt
    53041 ctggtaagct tccggagaga ggcccgcatc agcgattgcc tgctccatag aaaggaatgc
    53101 ataaatggat gcgtcgctca taaagcgcac aactttgcgg tcaatgaggc cagtggtatc
    53161 cagttttatg ttgccccaga cgtggctacg catgccggaa tccttcagct cctgagagaa
    53221 agtgatccct gaacgtcctt cacgcagaga tgccaggact tcctgctggt tattaccgat
    53281 gctggaaaca atgcccaggc cagtaatcac tgcacgtttc attcaatacc tctgtaagtc
    53341 gcacatagag taagtttcga atgcacaata gcgtacactt gtacgccgaa caagtccgat
    53401 cagccattta atagagatat ttgcgcagcc ttacacacat cgctaagatc gagccaccgc
    53461 ctgtaagacg agtaacttac gtgaaacact actccataca acctgccaac ctcgaattta
    53521 atgctgaggg tacacctgtt tcccgagatt ttgacgatgt ctatttttcc aacgataacg
    53581 ggctggaaga gacgcgttat gtttttctgg gaggtaacca attagaggta cgctttcctg
    53641 agcatccaca tcctctgttt gtgatagcag agagcggctt cggcaccgga ttaaacttcc
    53701 tgacgctatg gaaggcattt gatcagtttc gcgaagcgca tccgcaagcg caattacaac
    53761 gcttacattt cattagtttt gagaaatttc ccctcacccg tgcggattta gccttagcgc
    53821 atcaacactg gccggaactg gctccgtggg cagaacaact tcaggcgcag tggccaatgc
    53881 ccttgcccgg ttgccatcgt ttattgctcg atgaaagccg cgtgacgctg gatttatggt
    53941 ttggcgatat taacgaactg accagccaac tggacgattc gctaaatcaa aaagtagatg
    54001 cctggtttct ggacggcttt gcgccagcta aaaacccgga tatgtggacg caaaatctgt
    54061 ttaacgccat ggcaaggttg gcgcgtccag gcggcacgct ggcgacattt acgtctgccg
    54121 gttttgtccg ccgcggtttg caggaagccg gattcacgat gcaaaaacgt aagggctttg
    54181 ggcgcaaacg ggaaatgctt tgtggggtga tggaacagac attaccactc ccctgctcca
    54241 cgccgtggtt taatcgtacg ggcagcagca aacgggaagt agcgattatc ggcggtggta
    54301 ttgccagcgc gttgttgtcg ctggcgttat tacggcgcgg ctggcaggta gcgctttatt
    54361 gcgcggatga agcccccgcg ctgggtgctt ccggcaatcg ccagggggcg ctgtatccgt
    54421 tattaagcaa acacgatgag gcgctaaacc gctttttctc taatgcgttt acttttgctc
    54481 gtcggtttta cgaccaatta cccgttaaat ttgatcatga ctggtgcggc gtcacgcagt
    54541 taggctggga tgagaaaagc cagcataaaa tcgcacagat gttgtcaatg gatttacccg
    54601 cagaactggc tgtagccgtt gaggcaaatg cggttgaaca aattacgggc gttgcgacaa
    54661 attgcagcgg cattacttat ccgcaaggtg gttggctgtg cccagcagaa ctgacccgta
    54721 atgtgctgga actggcgcaa cagcagggtt tgcagattta ttatcaatat cagttacaga
    54781 atttatcccg taaggatgac tgttggttgt tgaattttgc aggagatcag caagcaacac
    54841 acagcgtagt ggtactggcg aacgggcatc aaatcagccg tttcagccaa acgtcgactc
    54901 tcccggtgta ttcggttgcc gggcaggtca gccatattcc gacaacgccg gaactggcag
    54961 agctgaagca ggtgctgtgc tatgacggtt atctcacgcc acaaaatccg gcgaatcaac
    55021 atcattgtat tggtgccagt tatcatcgcg gcagcgaaga tacggcgtac agtgaggacg
    55081 atcagcagca gaatcgccag cggttgattg attgtttccc gcaggcacag tgggcaaaag
    55141 aggttgatgt cagtgataaa gaggcgcgct gcggtgtgcg ttgtgccacc cgcgatcatc
    55201 tgccaatggt aggcaatgtt cccgattatg aggcaacact cgtggaatat gcgtcgttgg
    55261 cggagcaaaa agatgaggcg gtaagcgcgc cggtttttga cgatctcttt atgtttgcgg
    55321 ctttaggttc tcgcggtttg tgttctgccc cgctgtgtgc cgagattctg gcggcgcaga
    55381 tgagcgacga accgattccg atggatgcca gtacgctggc ggcgttaaac ccgaatcggt
    55441 tatgggtgcg gaaattgttg aagggtaaag cggttaaggc ggggtaatct gctctcgcag
    55501 tgtttgccgg atgcggcgtg aacgccttat ccaacctacg tgtgacgtgt aggcctgata
    55561 agacgcgata agcgtcgcat caggcatggt gctccagacg ccgcaactta cagctgtgac
    55621 gcctgctgaa acaggttttc ccacatatcg gtcaccagcg cctggtcacg cggcgacaat
    55681 tcaccggcac caatggcttt ttccagactc tggctaacgg tcgtatgcac cgcctgagcg
    55741 gagtggtcat caccactttc cagttctgcg atggctaatg tcaggtggcc acgcaaatac
    55801 ccactggcaa acaactcatc atcactggca tggtcaacca tgccgtcgat taatgccaga
    55861 atgcgtgatt caaactccgc gatcatcttc tttcctcatt gtaaaacgtg gcgctggctt
    55921 cagttgagcg cttccggcca cgggaactgt tccgccgtaa gttccggcgt gtgataataa
    55981 ttttgtagtg ctttaatgaa gcgtgccggg cgttccggaa tgccatttgt cagataatcc
    56041 atcacctgcg catgtacccg ccgttgaaac accacacgat ccggttcgaa atccccttcc
    56101 agattgtcgc aactgacgtt aaacggatat ccagccgcca cgcagaataa ccagtcgaac
    56161 gcctgcggct tcacttcaac atcttcaaac tggctttgcg tctgggcatc gcgtccgtcc
    56221 gggcaatacc agtagccgaa atcaaccagc tcacggcgcg cttttccggc aatacaccag
    56281 tgcgaaatct catgaattgc gctggcataa aagccatgag caaagacgat tcggttatac
    56341 ggtacttccg catcagcagg aagatagatc ggttcgtcgt cgcctttaat cagacgggta
    56401 ttaaattcat cggcaaagca gctattaaaa atttcaatca actgctcgta gtggtgtgta
    56461 ctgttcatta gttcatcccc aaccagtgga ggatctcctg tccgtggcta tcgtaaagta
    56521 atttggcact catcaccgcc gagacgataa caatcatcgg gcggatcagc ttttgtcctt
    56581 tgctcaacac caaccgtgaa cccatgcgcg cgccgaggaa ctgccccacc agcatgacaa
    56641 agcccgtcgc ccaaatcact ttgccgccga gaataaacag cagcagaccg ccaatgtttg
    56701 acgttgcgtt gagtaatttg gcgtgagccg tggctttggc gaggttgaat ccgcacagca
    56761 taacgaaggc cagcgcgtaa aacgatccgg cagccgggcc aaagaatccg tcataaaaac
    56821 cgacgcagcc accggcaatt aacgcgaagg gtaaaccgta catccggcgc tggcgatctt
    56881 cttcacccag ctttggcatc agcaagaaat agaggccgat acaaatcacc agaatgggca
    56941 aaatctgccg taagacatca gcctgaacgt actgtaccaa cagtgcgccg ctcattgagc
    57001 cgacaaaggt catggcaata ttgagtttct gatcgcttaa actaaccact ttgcggcgaa
    57061 taaagtagat agtggcggaa atagagccgc cgcaggcttg cagtttattg gttgccagcg
    57121 cgttagcggg agacattccc gcagccatca atgccggaat ggtgagtaac ccaccgccac
    57181 cggcaatcga gtcgataaat cccgccagca tggcgacaaa aaagagaact cccagcaaca
    57241 gcggagaaac cataaacagg ctattaaacg tttccattag atcacgtgct catccagtag
    57301 cgcctggcag gaaggcggca acggaggcgg tgtcttcttc tcaggctttg ttgttcccgg
    57361 ttttggaggt tcaaaccagc tttgcagttc tgccccgcaa ccatcgcctg atggcggtaa
    57421 aggttgatct tcacactcca gactatcggc agggcaacgt aatcgtacat gcatatgcgc
    57481 gcgatgctgg aaccagggtc gcactttgcg caaccagtcg cgatcggtgc ccgcatcaag
    57541 gcaaagttgt tgtttaatcg ccggattaac aaaaatgcgc gtgacgtctt tgtcctgggc
    57601 ggcgagtttg atcaagctga aaatttctgg cttccacagc gtggagacaa cgtgtttacc
    57661 gtcgcgggaa actaagtcca gtgcttgcgg gcgcaagagc tgcgcggagg tccagcgagt
    57721 tttcggcagt tgcagaaaga tatcgacatc cagtccggtc tggtggctgg catgaccgcc
    57781 gttgaaacgc ccaccagcgg gcatccccat atcgccaatc agcaccgtac ccatgcccag
    57841 attgctcacc tggctactca gacgctggat aaacatcacc agatccgggt gaccgaaata
    57901 gcgacgctga tcggtacgca tgacctgata atgttcggac tgtatcggca acgtgtcagc
    57961 gccgacaata cagccattag aaaaactgcc tatcgattgt gcgctacccg gcacaggttg
    58021 ggttattttt tgccacggcg tcgctgccag gctagcacta ctggcaagca gagccagcag
    58081 cgcaatcgcg gttttgttca ttttttacca gcgtggaata tcagtcttca catcggcatt
    58141 ttgcgcccgc tgccgtaaca ggtgatccat taaaacgatc gccagcatcg cttcagcgat
    58201 cggcactgca cggatcccga cacagggatc gtgacggcct ttggtgatca tctcaacttc
    58261 ttcgccaaag cggttaatag tgcgccccgg cacggtaatg ctggaggtcg gtttcagcgc
    58321 catatgagca atgatttgct gcccgctgct gataccgccg aaaatgccgc ccgcatggtt
    58381 gctctggaaa ccgtctttgg tgatttcgtc gcggttctgg ctgccgcgca gcgccactac
    58441 gtcaaaaccg tcgccaattt ccacgccttt caccgcgttg atgctcatca gcgcatgggc
    58501 gatgtcggca tccaggcggt caaagaccgg ctcgccaagt ccggcgggga caccactggc
    58561 aacaacggtg actttcgcgc cgatggagtc gccctctttt ttcagcgcgc gcatcagttc
    58621 atctaacgcg tcgattttgt cggggtccgg gcagaagaac ggattttgtt caaccagcga
    58681 ccagtctttg atttccagcg gaatgtcgcc catctgggtc aggcagccgc ggatttcaat
    58741 accaaatttc tcggcgagat attttttggc aatcgcccct gccgccacgc gcatggcggt
    58801 ttcgcgggcg gaagaacgtc caccgccgcg ataatcgcgc agaccgtatt tttgttcgta
    58861 ggtgtaatca gcatggcctg gacggaagac gtctttaatt gcgctgtaat cctgagaacg
    58921 ctggtcggta ttttcgatca acaagccaat gctggtgcca gtagtaacgc cttcaaaaac
    58981 accggagagt attttgacct gatccggctc gcggcgctgg gtggtatagc gcgatgtccc
    59041 agggcgacga cgatcaaggt catgttgcag gtccgcttcc gtcagcggaa tgcctggcgg
    59101 aacaccatcg acgatgcagc cgagcgccag cccgtgcgat tcgccgaagg tggttacgcg
    59161 aaagagttgt ccaattgtgt ttccagccat cacggctccg ttattgttgt gtttgcgtgt
    59221 ttacttaatc tttataaatc gcgaaatgtt ctcgtgcggc aataagctgc tctttagtga
    59281 gcataaacac accatcgccg ccgttatcaa actccagcca ggtgaacgga acatcgggat
    59341 attgttccat aagatgtacc atgctgttgc ccacttcaca aatcaacacg ccatcatcag
    59401 caaggtaatc tgccgcgtta ccgagaatgc gacgcgtcag tttcaggccg tcggtgccag
    59461 atgccaggcc cagttccggc tcgtgacggt attcgtttgg caggtcggac atatcttccg
    59521 cgtcgacata cggcgggtta gtgacaatca gatcgtattg cactttcggc aaatcacgga
    59581 acagatcgga acgaatcgga atgacgttgt ggatcagacc gtgttcttcg atgttctgtt
    59641 cggcaaccgc cagcgcgtcg ggagagatat ctaccgcgtc gacttccgct tccgggaagg
    59701 cataagcaca ggcaatggcg atgcagccgc taccggtaca catatctaaa atatgctgcg
    59761 gttgcttgct gatgagtccg gcaaatttat tgttgatcag ttcaccaatc ggcgagcgcg
    59821 gcaccagcac gcgttcatcg acgtaaaatt catgaccgca gaaccaggct ttgttggtca
    59881 ggtaagccac cggaatgcgc tcgttgacgc ggcggatcac gcgttcaaca atacggtgtt
    59941 tttcgctgga ggtcagacgc gcggtgcgca tatcttccgg gatatccagc ggcaggtaga
    60001 gcgatggtaa caccagctgt acggcttcat cccacgggtt atcggtgccg tgaccgtacc
    60061 agatatttgc cgcgctgaag cggcttaccg accagcgcaa tatgtcctga atggtttgca
    60121 gctcatttac tgcttcatca acgaaaattt tatccacgta ttcctccagg gcatgatcgc
    60181 aataatttcg gcggctagtt tgccatgaag atgacgataa atcagcattc acgcgcggtg
    60241 agtgaggaaa aatacgttta aaacgatcga ttgtgctacg agtcgggtaa actgtaggaa
    60301 aattagaaat agagacagat aaatgaaaaa gaaaacaaca cttagcgagg aggaccaggc
    60361 tctgtttcgc cagttgatgg cggggactcg caagattaag caggacacga ttgtccaccg
    60421 accgcagcgt aaaaaaatca gcgaagtgcc ggtgaaacgc ttgatccagg agcaggctga
    60481 tgccagccat tatttctccg atgagtttca gccgttatta aataccgaag gtccggtgaa
    60541 atatgttcgc ccggatgtca gccattttga ggcgaagaaa ctgcgccgcg gcgattattc
    60601 accggaattg tttttggatt tacacggtct gacgcagctg caggccaagc aggaactggg
    60661 cgcgttgatt gccgcctgtc gccgcgaaca tgtgttttgt gcctgcgtga tgcatgggca
    60721 cgggaagcat attttgaagc aacaaacacc gctgtggctg gcacaacatc cgcatgtgat
    60781 ggcatttcac caggcaccga aagagtacgg cggtgatgcg gctttgttgg tgttgattga
    60841 agtggaagag tggttgccgc cggagttgcc ctgaatgctg gcagcaaatg ccggagcagc
    60901 gtaaacgcct tatccggcct acaaatatgt gataattcaa taaattgcaa ttcacttcgt
    60961 aggcctgata agacgcatca gagcaatact acgaacttca gttcattcaa tacatactga
    61021 aaacgacacg tcgaatcaat cggttgccca ctactggtat tcacattcac cacaatatcg
    61081 tgtagtactt tatccgttac cggatgacga gttggcgtaa ccgttaccgt tgatttatca
    61141 caaccgtaag aggtaaaggt taacaattct gataccgcgc ggcagtatgc tcctctgcca
    61201 tcattatcag aaaaattact gccatcacag agtctgccgc cttccatcag gtaggtcgaa
    61261 agcgtggtgg tcactgttga ccccgtggac tgaattcgta acgccttatt atggctgaca
    61321 taattttcac tccaggagat atcgtatgaa gcgaccggta tcgttgggca tattgtgtta
    61381 tgcactgtag gggccgtata gcttccaaat ccgggtaaat ctgtatagga ataaaaggat
    61441 gcgatccaag ggcaaccata atcatcagat gcaacggtaa ttttaaacgt catatatgca
    61501 caacgaaaaa ttttcgtatc tatgccaggg caatttgagt tattaattgt atcaaagtca
    61561 gtcagtacca aagttggagc tgaacccgac ccagaccagg aaataacccg caacgtatcg
    61621 cctttatacg caaaagaatc atatttccca tatgcaacct cctttggcgt tatcaccgta
    61681 acattgaaaa cactgctcgc tacagcaccg gttaccttgt ccatggcata ataaactctc
    61741 acactgtcaa aactcgcact cataagttta tctccagcat aactcttaga tgcgccgaca
    61801 acactcagaa cgagcagcat taggctcgcg aaaattatgg tccatttttt catttcagaa
    61861 atactcaaag gttgccgtca ctgtggcact aaagtctccg gacgttacat ctcgcccgct
    61921 ctttgcctgt aaccaggtat taaaatttag gctgttattt ccctgattca gcgggagcgt
    61981 gacgcctgtt gtattgttga ttggcaccga tgtgcctgcc gcagtttcaa taccaatccc
    62041 agcgccggat gctgatgacg aggagtcaaa ggccagaaag ccgggtagag cactatcttc
    62101 tgttcctgtc aacgttactt tgacattaaa caatgtagaa ctatgacaat ctttcaattg
    62161 aaacaccaat ggcactcgct ctgactgccc aaggttcatt aaatcactgg cggcaatggc
    62221 aggaaaatgt acttctgcca actggctacc attaatcact ggcgtacacg atttactgat
    62281 taaattgccg gtaaagtgca aattatctcc tgctattgtg gttccactgc ccagcaacat
    62341 caggctacta ataaaaaaca acgtacgctt tttcttcatt ggtaatctac ctgtaacgtg
    62401 gcccaggcct caaagtccgc ctctggcaga atgacaccac tcttttttac tggcacagcc
    62461 gttaacaccg gtaaatcggt ttcattcacc acaagtggcg tattgatcgt aaaactctgc
    62521 cctgcctgtt tgagctgaat gcctaaacca gtgatattgc tgcttactgc agcattatca
    62581 aaatcgcttg gtgtgccact ccagctcaag gtcatttcca ttaatgggtc gcgaaccgcc
    62641 gtatcgcagg taatggaata cggcacggtt tgctcataat tgctgccatt aatgtcatca
    62701 ataagcacat cctgaaaagt tatttcaatg gtttgagcgt tgctaatcgt gcagttaggc
    62761 ggctgcacta aataaccgtg aaaagagacg tttgagagtg ccatcgagca aaagccccac
    62821 agcaaaataa gcgagatccg tttcattggt aatccaccac aattgtggca ctggcgttaa
    62881 attcagctgc cgctaacgtt gtaccactct gtttcaccgg cacagcttcc agaacgggaa
    62941 ctcccgagga aaaattaaat ggcaaccatg agccagaggt taagtcgagt attgtgtgat
    63001 cactactttt ctgaacccga ataccaaacc caggaatacc cgtgctgata actgtttcac
    63061 cgttaatcac gaccgttgtc gcctgcatct gtagacgcag gtcatccatt gccagattgt
    63121 tacaaacaag cgagtagtca atgggacgtt tataatcaac cctattgatc ttattaatga
    63181 atacattacc aaactcaact gccgcaccat ttaccgtaca aggaggaggg gcatcgacaa
    63241 gcacccgtaa attcatactt actgacttac tcgctgcaac tgcaaagccg ctaccaacgc
    63301 aacatatcag taacccagtt accgcccaga cagaacaaga tcctttcata ctggctctcc
    63361 cggcctcagc ctttgcggct ctgctcttca tcgacctagc aactgttacc gttgcaagtg
    63421 aaaaagagcg gcatccgagc gccatagtcg ttgacatagg tcagtaccgg cacgctaccc
    63481 atcttcacat tcagcggtgc agaggttttc ggcgacatca ctattggcga gaaaccggct
    63541 gccggattgc catttttctg tgtgctggca ttactgaaaa tgacgtaata cgacgtcggg
    63601 ttattgacgg taaattcctg accgttgcgg gttaaagtaa tcttatattg ctaggggttt
    63661 ttcatgctga cgttctccag cgccttcggt cgccagaaca acttaatacg cgtttgtagt
    63721 gctatttgca gcgtgttggc tttgtttgat tttggcggga tctcacgcac gttgaagtag
    63781 aacaggcttt cacgatcggc aggcaatttg ttgatatccg gcattccctg cacttttacc
    63841 tgcccgttca tcattgaatc gatgcgttgc accggtggca gaacagttaa cggtgaactg
    63901 atcttgttac ccttttcatc ttcgatccag ctttgggcca gataaggcag tttcggatcg
    63961 ttattgcgca acgtcacgct gattgatttg tcgctttcat taaatatcaa acgggtacga
    64021 tctggcgtta cagaagcctg ggcagacaaa ctggcagcgc taagcagtaa cgctagcgtc
    64081 gtagcaccca atttggcaga gcaaagtaaa tcagacatgg atcattcctt aatcacgtta
    64141 ttggttagct gaaacagaaa cagggggatt ggtgggtgtc acctgttgcg tctgctcctg
    64201 aatgacaggc tttatgtcat caggaacaac cggcgcgacg ttgcctttat gctggcatgg
    64261 cagtaacaag ccgttaaaca gatcggcagg cagcgggtcc ggcaggttga tatcgcaatg
    64321 cgcaacacca ctccagaaca cactcatgtg ttcgccaggt ttcactccag ccagataaac
    64381 attgccgtca tcatcgacaa ggcccactgt ctgctcgtta tcatttttta cttctgcgcc
    64441 aaacggagga tggctgccgt cttgtaaacg cagcaccgcc atcgcttttt gaccactgat
    64501 gacggcaaat ttgcggtagc caatcgcccc ttcagttagc gtggcttgta ccaccgactg
    64561 ggttgcttcg gcgttttccg gcagtttgtt gaggtcgata tacgcctgat tgcggtaata
    64621 gttattgacg tcagaaacga cggctttacc aaacatattg gtataaacag cagcaccgtt
    64681 accttctacc ggaacatcgg caacgccatc ggcatcaatc aacaagcgtg tcccgcccat
    64741 attctgggta cggtgaagtg cgccaccgtg ggcagtaagc gttgcgccgc cctgtaacga
    64801 caagcccgca gaggtgtatt gcccttcatg gtagttcgca ctgaggtcaa cctgcgccag
    64861 cgaaccatca tggctgtaat agccgtcaac gctggtgtgt ttgtcactgg tgccgacgtt
    64921 caactgatag tgagtcgcgt catcgacacg gctgaaataa ccgacctgac tgctgtccgt
    64981 cccactgcca tagtaccgtt atagctaacg gtactgttgt cgccccacgg catactgagc
    65041 gaaatgtaca tgcctttgtc ggcctggttg tcatactcgt aacggtagcc agtcatcgag
    65101 atgctgacat tacgaatact gcccatattg aaatagtgcg agagcatgat gttgtagttt
    65161 gtctgctcct cgcgatccca gtaggtatgg cgggtatagt tgagataaac cgaaacaccc
    65221 gcatcgcgga agttctggtt ataagtggcg gtgtacatct ctttgtcgtt gcccgtgcgt
    65281 accattccgc tgtcactggc atccagatac tcgctcatgg tcataaagtt ctcttccgag
    65341 aagcgatatc cagcaaaagt aacgcggctg ttgagctggt caaagtcttt ggaataactc
    65401 acacggaagg agttaccgtc cagcgaacct ttgccataag cggtgtcttt atccagtttg
    65461 gtgtgcgagt gagtaacatc aaacgcgacc gcgccgaatg tagacaaatc gcgaccgaca
    65521 ccaagcgccg cagactgata gttttcatct cccagtgcgc cgccatataa cgaccagccg
    65581 ttagcgatcc cccaggaagc ttcagcacca gaaaaaaatt caccctcgac atggtgtccc
    65641 cactcttgcg gacggcccat catgatctta tagcgaacct gacctggacg agtgaggtat
    65701 ggcatcgagg cggtgctgat gtcatattcc tgcacctggc cgttctgttc ttcaatgcga
    65761 atatgcaacg taccggagac agaatcacca agatcctgaa tacgaaacgg tccagccgga
    65821 acctgcgttt cgtaaatcac acgccccatc tggctgacgg tcacttttgc tgtggtgtgt
    65881 gccacgccgg aaatgtctgg cgcgtagccg cgcagattgg gaggcaacat ttgatcgtca
    65941 gtactgacac tgccaccaac atagttaaaa ccatcaaaaa tatcggatct gaggtaatcc
    66001 tcgcccagcg ccagtttggc ttttaatgat ggtaacgccc gcctggcata gtagcgactc
    66061 cactcccatt tttttgagtt tcatcgccgc tgaattcatc gtcatcatta ctgcgagtat
    66121 gttgatagtt agtctgccag tcagcacgca tacgccacgg ccctaggtta accccaaccg
    66181 tcccgttgcc gctgatctcg ttactatcat caccgccatt ttcttcgtgc cgggtttgtg
    66241 cgttgatgct gtagtccgcg acgatcccgg agatgccgtc atcccaacgt gaaggcggat
    66301 ccctgtcggg ataagtatat tcgaggtaag cctgtggcag tgaaatgact aatgcggact
    66361 ggcttaaatc agccttaatt tccatgcctt ccagttgacc ggatttcagg catttagcat
    66421 cgtggctcca ttgcagattt ttcgccacgt cttcttttaa accaaactgc gctaccagtt
    66481 ccggtgtcag acaagcatag cttttgctcg cgtcatcttc accagcatac cagtaaatat
    66541 cgtactcttc cgccaatggc tgtttattta gttgaacctg taaattgtat tttccgggct
    66601 caacatatcc ctggctggaa aaacgcttca tatcaatttt tgtgtcgcct tttaattcca
    66661 gaaaacggga atcaaactga atgtcgtctt cagcccagac actgctataa ctccctgaca
    66721 ttgccagcgc aatgcaccac ggaagaatcc gtaatcgaaa aagagaatgg tcaggcatac
    66781 acgtttctat ccgtttttat tcttgttcgg tcaaaataca tttaaatccc ccatagcagt
    66841 gtcgctatgg gggtatgcct tacaggtaag taatctggaa ggtggtattt gcttcaaaat
    66901 tacccagatc tggcgcatca gctgcgccga ccagccaggc gttaaagttc agtgtctgct
    66961 tcgctttgcc tttgttggta gaagtgtctt tcactatttt ctggtcaata cccatgccgc
    67021 ttttgtaaga ggtacccaga gagtcaccga tcgccaggct aacattgtta aatgccgcac
    67081 cggtatcggt attgaaaatg gtgtaataat tgccgctatt tgcagaagaa acggtaccag
    67141 taaaggtagt ggtcattgtt tcctgagtat caaatacgca atcttgcaga cgaatctgga
    67201 aacttttcgg cgtggtggtg ccgttatttt tcagtttttc ggcaccgata tcacccatat
    67261 caacttccag tttgtgatcg tccggaacga tagaacacac tgagtcttca atggtgccgt
    67321 aaaaacgtgc agtaccgttg ttacccgcag cgattgttgc cgtagaggtg aacacgccca
    67381 tcaccattgc cgcagcaata gctgttttaa caaacttact cattatttgt ccttaaacta
    67441 taaataatta aaaatataaa cattgcaata cattgattca gtcaatagcc aatgtttata
    67501 cccagccatt gaatacggca tgcgacccaa aaagaacagg tcgtagaaaa ttgtattcat
    67561 tggtgtgggg ccgcatagtg atgtttccac acccctgatc caatttaatt ttactaaaga
    67621 gtaaatgtag atttaattaa tatattgaca gaagaaaggt tatcttaagg a
//
LOCUS       DAJTSA010000008        66971 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000008 DAJTSA010000000
VERSION     DAJTSA010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66971
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.008"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(65..1069)
                     /gene="cra"
                     /locus_tag="OO369_000564"
     CDS             complement(65..1069)
                     /gene="cra"
                     /locus_tag="OO369_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR5980951.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1151..1237)
                     /locus_tag="OO369_000565"
     CDS             complement(1151..1237)
                     /locus_tag="OO369_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR5980952.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="OO369_000566"
     CDS             complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="OO369_000566"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5980953.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="OO369_000567"
     CDS             complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="OO369_000567"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR5980954.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3586..3630
                     /locus_tag="OO369_000568"
     CDS             3586..3630
                     /locus_tag="OO369_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5980955.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="OO369_000569"
     CDS             complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="OO369_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR5980956.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5388..5474
                     /gene="leuL"
                     /locus_tag="OO369_000570"
     CDS             5388..5474
                     /gene="leuL"
                     /locus_tag="OO369_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR5980957.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5567..7138
                     /gene="leuA"
                     /locus_tag="OO369_000571"
     CDS             5567..7138
                     /gene="leuA"
                     /locus_tag="OO369_000571"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR5980958.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7138..8229
                     /gene="leuB"
                     /locus_tag="OO369_000572"
     CDS             7138..8229
                     /gene="leuB"
                     /locus_tag="OO369_000572"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR5980959.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8232..9632
                     /gene="leuC"
                     /locus_tag="OO369_000573"
     CDS             8232..9632
                     /gene="leuC"
                     /locus_tag="OO369_000573"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR5980960.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9643..10248
                     /gene="leuD"
                     /locus_tag="OO369_000574"
     CDS             9643..10248
                     /gene="leuD"
                     /locus_tag="OO369_000574"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR5980961.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10297..11475)
                     /gene="setA"
                     /locus_tag="OO369_000575"
     CDS             complement(10297..11475)
                     /gene="setA"
                     /locus_tag="OO369_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR5980962.1"
                     /translation="MIWIMTMARRMNGVYAAFMLFAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="OO369_000576"
     CDS             complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="OO369_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR5980963.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11797..13452
                     /gene="sgrR"
                     /locus_tag="OO369_000577"
     CDS             11797..13452
                     /gene="sgrR"
                     /locus_tag="OO369_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR5980964.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13486..13585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13616..14599
                     /gene="thiB"
                     /locus_tag="OO369_000578"
     CDS             13616..14599
                     /gene="thiB"
                     /locus_tag="OO369_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR5980965.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14575..16185
                     /gene="thiP"
                     /locus_tag="OO369_000579"
     CDS             14575..16185
                     /gene="thiP"
                     /locus_tag="OO369_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR5980966.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16169..16867
                     /gene="thiQ"
                     /locus_tag="OO369_000580"
     CDS             16169..16867
                     /gene="thiQ"
                     /locus_tag="OO369_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR5980967.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16981..17745)
                     /locus_tag="OO369_000581"
     CDS             complement(16981..17745)
                     /locus_tag="OO369_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5980968.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17831..18709)
                     /gene="araC"
                     /locus_tag="OO369_000582"
     CDS             complement(17831..18709)
                     /gene="araC"
                     /locus_tag="OO369_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR5980969.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19048..20748
                     /gene="araB"
                     /locus_tag="OO369_000583"
     CDS             19048..20748
                     /gene="araB"
                     /locus_tag="OO369_000583"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR5980970.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20759..22261
                     /gene="araA"
                     /locus_tag="OO369_000584"
     CDS             20759..22261
                     /gene="araA"
                     /locus_tag="OO369_000584"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR5980971.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22461..23156
                     /gene="araD"
                     /locus_tag="OO369_000585"
     CDS             22461..23156
                     /gene="araD"
                     /locus_tag="OO369_000585"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR5980972.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23231..25582
                     /gene="polB"
                     /locus_tag="OO369_000586"
     CDS             23231..25582
                     /gene="polB"
                     /locus_tag="OO369_000586"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR5980973.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25747..28653
                     /gene="rapA"
                     /locus_tag="OO369_000587"
     CDS             25747..28653
                     /gene="rapA"
                     /locus_tag="OO369_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR5980974.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28665..29324
                     /gene="rluA"
                     /locus_tag="OO369_000588"
     CDS             28665..29324
                     /gene="rluA"
                     /locus_tag="OO369_000588"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR5980975.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="OO369_000589"
     CDS             complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="OO369_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR5980976.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30511..32865
                     /gene="lptD"
                     /locus_tag="OO369_000590"
     CDS             30511..32865
                     /gene="lptD"
                     /locus_tag="OO369_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR5980977.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32918..34204
                     /gene="surA"
                     /locus_tag="OO369_000591"
     CDS             32918..34204
                     /gene="surA"
                     /locus_tag="OO369_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR5980978.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34204..35193
                     /gene="pdxA"
                     /locus_tag="OO369_000592"
     CDS             34204..35193
                     /gene="pdxA"
                     /locus_tag="OO369_000592"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR5980979.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35190..36011
                     /gene="rsmA"
                     /locus_tag="OO369_000593"
     CDS             35190..36011
                     /gene="rsmA"
                     /locus_tag="OO369_000593"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR5980980.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36014..36391
                     /gene="apaG"
                     /locus_tag="OO369_000594"
     CDS             36014..36391
                     /gene="apaG"
                     /locus_tag="OO369_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR5980981.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36398..37240
                     /gene="apaH"
                     /locus_tag="OO369_000595"
     CDS             36398..37240
                     /gene="apaH"
                     /locus_tag="OO369_000595"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR5980982.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37318..37797)
                     /gene="folA"
                     /locus_tag="OO369_000596"
     CDS             complement(37318..37797)
                     /gene="folA"
                     /locus_tag="OO369_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR5980983.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="OO369_000597"
     CDS             complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="OO369_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR5980984.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="OO369_000598"
     CDS             complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="OO369_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR5980985.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40421..41803)
                     /locus_tag="OO369_000599"
     CDS             complement(40421..41803)
                     /locus_tag="OO369_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5980986.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPYVLVMIGVALEQLTPAL
                     KLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMCVSSP
                     VELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADLVGYW
                     LYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFGEPVA
                     FDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLGVGKN
                     AALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGIWLVV
                     MAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPIFINN
                     YGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISLFSES
                     ILLTISRVNI"
     gene            complement(41862..42149)
                     /gene="fixX"
                     /locus_tag="OO369_000600"
     CDS             complement(41862..42149)
                     /gene="fixX"
                     /locus_tag="OO369_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR5980987.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42146..43432)
                     /locus_tag="OO369_000601"
     CDS             complement(42146..43432)
                     /locus_tag="OO369_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5980988.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43483..44424)
                     /locus_tag="OO369_000602"
                     /pseudo
     CDS             complement(43483..44424)
                     /locus_tag="OO369_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44439..45209)
                     /locus_tag="OO369_000603"
     CDS             complement(44439..45209)
                     /locus_tag="OO369_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR5980989.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45565..47079
                     /gene="caiT"
                     /locus_tag="OO369_000604"
     CDS             45565..47079
                     /gene="caiT"
                     /locus_tag="OO369_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR5980990.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47110..48252
                     /gene="caiA"
                     /locus_tag="OO369_000605"
     CDS             47110..48252
                     /gene="caiA"
                     /locus_tag="OO369_000605"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR5980991.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48381..49598
                     /gene="caiB"
                     /locus_tag="OO369_000606"
     CDS             48381..49598
                     /gene="caiB"
                     /locus_tag="OO369_000606"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCR5980992.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49672..51225
                     /gene="caiC"
                     /locus_tag="OO369_000607"
     CDS             49672..51225
                     /gene="caiC"
                     /locus_tag="OO369_000607"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR5980993.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51334..52119
                     /gene="caiD"
                     /locus_tag="OO369_000608"
     CDS             51334..52119
                     /gene="caiD"
                     /locus_tag="OO369_000608"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR5980994.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52125..52715
                     /gene="caiE"
                     /locus_tag="OO369_000609"
     CDS             52125..52715
                     /gene="caiE"
                     /locus_tag="OO369_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR5980995.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52801..53196)
                     /gene="caiF"
                     /locus_tag="OO369_000610"
     CDS             complement(52801..53196)
                     /gene="caiF"
                     /locus_tag="OO369_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR5980996.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53458..56679)
                     /gene="carB"
                     /locus_tag="OO369_000611"
     CDS             complement(53458..56679)
                     /gene="carB"
                     /locus_tag="OO369_000611"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR5980997.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56697..57845)
                     /gene="carA"
                     /locus_tag="OO369_000612"
     CDS             complement(56697..57845)
                     /gene="carA"
                     /locus_tag="OO369_000612"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR5980998.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58301..59122)
                     /gene="dapB"
                     /locus_tag="OO369_000613"
     CDS             complement(58301..59122)
                     /gene="dapB"
                     /locus_tag="OO369_000613"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR5980999.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59289..60203)
                     /gene="rihC"
                     /locus_tag="OO369_000614"
     CDS             complement(59289..60203)
                     /gene="rihC"
                     /locus_tag="OO369_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR5981000.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60269..61219)
                     /gene="ispH"
                     /locus_tag="OO369_000615"
                     /gene_synonym="lytB"
     CDS             complement(60269..61219)
                     /gene="ispH"
                     /locus_tag="OO369_000615"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR5981001.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61221..61670)
                     /gene="fkpB"
                     /locus_tag="OO369_000616"
     CDS             complement(61221..61670)
                     /gene="fkpB"
                     /locus_tag="OO369_000616"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5981002.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61757..62251)
                     /gene="lspA"
                     /locus_tag="OO369_000617"
     CDS             complement(61757..62251)
                     /gene="lspA"
                     /locus_tag="OO369_000617"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR5981003.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62251..65067)
                     /gene="ileS"
                     /locus_tag="OO369_000618"
     CDS             complement(62251..65067)
                     /gene="ileS"
                     /locus_tag="OO369_000618"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR5981004.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65110..66051)
                     /gene="ribF"
                     /locus_tag="OO369_000619"
     CDS             complement(65110..66051)
                     /gene="ribF"
                     /locus_tag="OO369_000619"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR5981005.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66059..66277)
                     /locus_tag="OO369_000620"
     CDS             complement(66059..66277)
                     /locus_tag="OO369_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR5981006.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66380..66643
                     /gene="rpsT"
                     /locus_tag="OO369_000621"
     CDS             66380..66643
                     /gene="rpsT"
                     /locus_tag="OO369_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR5981007.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16242 a        18234 c        17244 g        15251 t
ORIGIN      
        1 atgtccagtc ccgtactcta cgcgccagag ggaaattcac ctggcgcgta tttttgttcg
       61 cggattagct acggctgagc acgccgcggc gatagagatt acgtttaatg cgcgttaaac
      121 caggttttgg cttacgcggt tcgtccaggc ttgccaggac aatctccagc acacgctctg
      181 cgacatcgcg gtgacgttga gccactgcca gcaccggaca ctgtaagaag tcgagcagtt
      241 cgttatcgcc aaaggtggca attgccaggt cagaaggcag tttgccgtcg cgacgcagcg
      301 tgacatccat cactccttgc aacaacgcaa acgacgttgt gaacagcgcc tgcggcatcg
      361 gatgcgtttc cagccatttt tcgaataact gggcagccgc ctcccgctca tagctgttgg
      421 catacaggaa atgcacttcg cgcggatcat ctttccaagc agtacggaaa ccttgttcac
      481 gcaggaagct gacagaaagc tccggcagcg cgccaagata aagcaccgtc tcggcgggaa
      541 acttacgtaa ctcttccgcc agcatttcgg catcatcctg atcggcacca accacgctgg
      601 tgaaatgttc acgatcgagg gcgcggtcca gcgcgacaat cgggaacggg tcgttagccc
      661 agcgttgata aaaaggatgc tcaggtggca acgacgtcga aacaataatg gcatcaacct
      721 gacgctgtaa aaggtgctca atgcagcgca tttcgttgtc tggctgatct tctgagcagg
      781 caatcagcag ttgataaccc cgttgccgcg cctggcgttc aagatagtta gcgatgcggg
      841 tatagctggt gttctccaga tcggggatca caagaccaat agaacgtgtg cgtccagcac
      901 gaagcccagc tgccacggcg ttcgggtggt aattgtgctc acgcaccaca gccatgactt
      961 tttcaacggt tttgtcgctc acacggtatt gcttcgcttt gccgttaata acatagcttg
     1021 cagtggtccg cgacactccc gccagccgag cgatttcatc cagtttcaca attgcccctt
     1081 gcgtaaaatg taaaaaccat agccattgta ctggtatggg ttaaattcat taacatctaa
     1141 gcgcagaaaa ttaactgcgg caactgcttt tattccaggt tcccactgat ttcgcaaaaa
     1201 aagcccaacg tgacatgttg ggctgcaaat tgcgcactga gatcattctc aacgcattat
     1261 tttatcgccg cgcgaaagtc cgaccacacc agagcgagca acttccacaa ttttcgccac
     1321 atcgcgaatc gatgctaaaa atgcatcaag cttatcgctg gtgcctgcta attgaacggt
     1381 ataaagcgag ggggtgacat cgataatttg cccacggaat atttccgtat tacgtttcac
     1441 ttcgtcacgc ccgtaaccgc tggcctgaat tttcaccagc atgatttccc gctcaacatg
     1501 cgcgccctgc cccaactcac tcacgcgcaa gacatcgacc agcttgtgta attgcttttc
     1561 gatctgctca agtacttttt catcgcccac ggtctggatg gtcatacgcg ataatgtcgg
     1621 atcgtcggtt ggcgcaacgg tcaggctttc aatgttgtag cctcgctggg aaaaaaggcc
     1681 aatcacgcgg gataacgcgc ctgattcatt ttcgagtaag actgataata tccggcgcat
     1741 aatcaggttc tctccgtttt gcttaaccac atttcatcca ttccgccccc gcgaatctgc
     1801 atcgggtaga cgtgctcact gccatcgacg gtaacatcaa caaacaccag gcgattattg
     1861 cgcacctgtt ccagcgcctc gctaagtttg ctttccagct catgcggatg agagatctgg
     1921 atcccgacgt gaccataggc ttccgccaga cggacgaaat cgggtagtga ttgcatataa
     1981 gattgtgaat gacggccgga atagatcatg tcctgccact gtttcaccat ccccagatag
     2041 cggttattga gattcaccac cagtacgggc aactcgtatt gcaacgcggt agacagttcc
     2101 tggatgttca tctgaatact gccgtcgcca gtgacgcaaa ccacggtttc ttctggcaac
     2161 gccattttga cgcccagtgc cgcaggtaaa ccaaaaccca tcgtgccgag accgccggaa
     2221 ttgatccagc gacgcggttt gtcgaatgga taataaagcg cagcaaacat ctggtgctgc
     2281 ccgacatcgg acgtcacgta agcgtcccca ttagtcaacc gccaaagagt ctcgatcacc
     2341 gcctgcggtt taatcttttc actgtgagtg tcatatttca ggcactgaag agcgcgccac
     2401 tgttcaatct gctgccacca gtcgcggatc tcatccagcg gttgatgggc ggattcttgc
     2461 gacaggagtt caagcatttg ttcgaggacc tggcgagcat cccccacaat cgggatatcc
     2521 gcagtcacgg ttttagaaat ggaagtagga tcaatatcga tatgcagaac agtggcattt
     2581 gggcagtact ttgccagatt gttcgtcgtt cggtcatcaa atcgtacccc gacggcgaaa
     2641 atcacatccg cgttatgcat cgtcatattg gcttcgtagg taccgtgcat tcccagcatg
     2701 cccagtgcct gacgatgcgt tgccggaaac gcccccattc ccatcaatga ggaaacaacg
     2761 ggcagattca acgcctccac cgtttctttc aactgctgat ggcagcccgc catgattgcc
     2821 ccaccgccta cgtagacaac cggttttttt gccgctacca gcgtttgcag agcacgctta
     2881 atttgccctt tatgtccggt agtagtggga ttgtaagaac gcatactgac cgactcaggg
     2941 cagacatagg gtaatttgtt cgccggatta agaatatctt tcggtaaatc aacgaccact
     3001 ggtcctgggc gaccacttgc cgccagccag aaagcctttt tcagcacctg cggaatgtct
     3061 tccgtttgct taaccagaaa actgtgttta accaccggtc gcgaaatccc caccatgtcg
     3121 cactcctgaa aggcatcgta acctatcaac gaggtcgcta cctgcccgga aaggacaact
     3181 aatggaatgg aatccatata agcggtggcg atgccggtaa tcgcattggt cgcccctgga
     3241 cccgacgtta ccagcacgac gccgacttcc ccggtcgcac gcgccaggcc atcggccata
     3301 tgcaccgccg cctgctcatg acgaactaat acatgatcaa taccacccac ggtatgcaat
     3361 gcatcgtaaa tatcaaggac tgcgcctccg ggataaccga atacttgttt aacgccctga
     3421 tcgataagcg atcggacgac catctcggct ccagacaaca tctccatggc ctgcctcact
     3481 gtttgacgga aaaaatgtgt aaaacacatt aaccgtccga tcttgcttaa gcaattggca
     3541 gccagagaca taaacgcagg gtaataagaa taaacaggag gaaaggtgaa aagaaacgat
     3601 aaaatcctcc attacgaagg attaaattga taataaatcg gctgaatccc acaacttaca
     3661 gcaatttctc tattttttta attaatccta aaatagagag tccacacatt cagaataaaa
     3721 ttctacattc aataaaaaat gcagaataaa ccagacattc atgtctggcc tattctgcat
     3781 tcagttagcg tttgcaaatt gagactaatt gttcttccat ccactgatgg cctttatcgc
     3841 gcccggcagc ttcatgccaa gagagataac aggttctgct gttttgtttt aacggcagcg
     3901 gtaatacctg taattctaag gattcagcga actcttcagc cagccaacgc ggcgcaatag
     3961 cgaccaaatg cgtttgcgac accacgctaa gtacgctcat cattgccatg ccctgatacg
     4021 cgatactggc ttgcttatct accgtgtcat accaaggttg actaaatgac gcgaaacgat
     4081 cgagcgaaac cgccgcatgt tgttcgttat aaacatcatg tttcagtaac gggcctttaa
     4141 ttgttggatg atttttgctg gctaccagca ccatttcatc tttaaataat ggcacgctgg
     4201 taaattcagg acgatggaag tcttcataac taatcacaaa ctccgtttcc tgataacgca
     4261 gctgatgttc agtgttctga tttaatgaag acttgaacat aacatgtata tttggcgcaa
     4321 tctgctcaat gtgattataa atctgcgagg tcagaatgct gtctaacggg ctgcaaacac
     4381 aaagatgaaa tacacgttca ctgctcgcgg gttcaaaacc tgaaccaggc aattcatttt
     4441 gtactagttg caatgcctgg cgaactgaac caaaaagttg aaatgcgcga gcagtcggtt
     4501 gaataccacg gccataacga acaaaaagct cgtcattaaa catcaccttc aggcgtgcaa
     4561 cagcgttact gaccgcaggt tgcgacatac ccagaacatg agcggcacgg gtaatgtttt
     4621 gctcctgcat cacggcatcg aaaacggtta ataagttgag atcgaccatg cgtagctgtg
     4681 gcttgcttaa ctccgccgtc tctgaatgat ctgtttgtac ctctggcata cttaactcca
     4741 ctgtcacact taactccctt tcccttattg gaatgcagag aaataatcct gaagaatatg
     4801 atttatcatg catataaaat aagaaaaagc aaaatgagta aaattcggaa aacataaaga
     4861 tacgggttct gataaaaaca gaatccattg ccgtggaatg agtcatttac cacagcataa
     4921 taatccataa tgttttgttg cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca
     4981 tctacaaaat ggattaaatg tgcttattta ataattaatt tatatattta atgcattaat
     5041 tcttaacatt aattgttcaa taatattcac caaatcaata tcaaaaaaaa tcgcaaaaca
     5101 tataattcaa tacaaatcat caggataggt tttgcaacgc gtgcattttg tccccttttt
     5161 cctcgttgat tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa aagcgtcggt
     5221 agttaagcag aaattaatat cgcttacttt aaccaccgta gcacaattag ctaattttac
     5281 ggatgcagaa ctcacgctgg cgggacgttt ttattgcgtc agggttgaca tccgtttttg
     5341 tatccagtaa ctctaaaagc atatcgcatt catctggagc tgatttaatg actcacatcg
     5401 ttcgctttat cggtctacta ctactaaacg catcttcttt gcgcggtaga cgtgtgagcg
     5461 gcatccagca ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg cgcgggtttt
     5521 tttatgcccg aagcgaggcg ctctaaaaga gacaaggacc caaaccatga gccagcaagt
     5581 cattattttc gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa gcttgagtgt
     5641 gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg tgatggaagt
     5701 cggtttcccc gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg cccgccaggt
     5761 taaaaacagc cgcgtatgtg cgttagctcg ctgcgtggaa aaagatatcg acgtggcggc
     5821 cgaatccctg aaagtcgccg aagccttccg tattcatacc tttattgcta cctccccaat
     5881 gcacatcgcc accaagctgc gcagcaccct agacgaggtg atcgaacgcg ctatctatat
     5941 ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt tcttgcgaag atgccgggcg
     6001 tacacccatt gccgatctgg cgcgagtggt cgaagcggcg attaatgccg gtgccaccac
     6061 catcaacatt ccggacaccg tgggctacac catgccgttt gagttcgccg gaatcatcag
     6121 cggcctgtat gaacgcgtac ctaacatcga caaagccatt atttccgtac atacccacga
     6181 cgatttaggc ctggcggtcg gcaactcact ggcggcggta catgccggtg cacgccaggt
     6241 ggaaggtgca atgaacggga tcggcgagcg tgccggtaac tgttcgctgg aagaagtcat
     6301 catggcgatt aaagttcgta aggatattct caacgtccac accgccatta atcaccaaga
     6361 gatatggcgc accagccagt tagttagcca gatttgtaat atgccgatcc cggcaaacaa
     6421 agccattgtt ggcagcggcg cattcgctca ctcctccggt atccaccagg atggcgtgct
     6481 gaaaaaccgc gaaaactacg aaatcatgac accagaatct attggtctga accaaatcca
     6541 gctgaatctg acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg atgagatggg
     6601 gtataaagaa agtgaatata atttagacaa tttgtacgat gctttcctga agctggcgga
     6661 caaaaaaggt caggtgtttg attacgatct ggaggcgctg gccttcatcg gtaagcagca
     6721 agaagagccg gagcatttcc gtctggatta cttcagcgtg cagtctggct ctaacgatat
     6781 cgccaccgcc gccgtcaaac tggcctgtgg cgaagaagtc aaagcagaag ccgccaacgg
     6841 taacggtccg gtcgatgccg tctatcaggc aattaaccgc atcactgact ataacgtcga
     6901 actggtgaaa tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg gtcaggtgga
     6961 tattgtcacc aactacaacg gtcgccgctt ccacggcgtc ggcctggcca ccgatattgt
     7021 cgagtcctcc gccaaagcca tggtgcacgt actgaacaat atctggcgtg ccgcagaagt
     7081 cgaaaaagag ttgcaacgca aaactcaaca caacgaaaac aacaaggaaa ccgtgtgatg
     7141 tcgaagaatt accatattgc cgtattgccg ggagacggta ttggtccgga agtgatgacc
     7201 caggcgctga aagtgctgaa tgccgtgcgc aaccgctttg cgatgcgcat caccaccagc
     7261 cattacgatg taggcggcgc agccattgat aaccacgggc aaccactgcc gcctgcgacg
     7321 gttgaaggtt gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg cccgaagtgg
     7381 gaacatttac caccagacca gcaaccagaa cgcggcgcgc tgctgccgct gcgtaagcac
     7441 ttcaaattat tcagcaacct gcgcccggca aaactgtatc aggggctgga agcattctgt
     7501 ccgctgcgtg cagacattgc cgctaacggc ttcgacatcc agtgcgtgcg cgaactgacc
     7561 ggcggcatct atttcggtca gccaaaaggc cgcgaaggta gcggacaata tgaaaaagcg
     7621 tttgataccg aggtgtatca ccgttttgag atcgagcgta ttgcccgcat cgcgtttgaa
     7681 tctgctcgta aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt gctgcaatcc
     7741 tctattttat ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga tgtcgaactg
     7801 gcgcatatgt acatcgacaa cgccaccatg cagctgatta aagatccatc acagtttgac
     7861 gttctgctgt gctccaacct gtttggcgac attctgtctg acgagtgcgc aatgatcact
     7921 ggctcgatgg gcatgttgcc ttccgccagc ctgaacgagc aaggttttgg actgtatgaa
     7981 ccggcgggcg gctcggcacc agatatcgca ggcaaaaaca tcgccaaccc gattgcacaa
     8041 atcctgtcgc tggcactgct gctgcgttac agcctggatg ccgatgatgc ggcttccgcc
     8101 attgaacgcg ccattaaccg tgcattagaa gaaggcattc gcaccgggga tttagcccgt
     8161 ggcgctgccg ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta tgtggcagaa
     8221 ggggtgtaat catggctaaa acgttatacg aaaaattatt cgacgctcac gtagtgtacg
     8281 aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca cctggtgcat gaagtgacct
     8341 caccgcaggc gttcgatggt ctgcgcgccc acggtcgccc ggtacgtcag ccgggcaaaa
     8401 ccttcgccac tatggatcac aacgtctcta cccagaccaa agacattaat gcctgtggtg
     8461 aaatggcgcg catccagatg caggagctga ttaaaaactg caaagaattt ggcgtcgagc
     8521 tgtatgacct gaatcacccg tatcagggga tcgtccacgt aatggggccg gaacagggcg
     8581 tcaccttgcc ggggatgacc attgtctgcg gcgactcgca taccgccacc cacggcgcgt
     8641 ttggcgcact ggcctttggt atcggcactt ccgaagttga acacgtactg gcaacacaaa
     8701 ccctgaaaca gggccgtgcg aagaccatga aaattgaagt ccagggcaaa gccgcgccgg
     8761 gcattaccgc aaaagatatc gtgctggcaa ttatcggtaa aactggcagc gcaggcggca
     8821 ccgggcatgt ggtggagttt tgcggcgaag caattcgtga tttaagcatg gaaggtcgta
     8881 tgaccctgtg caatatggcg atcgaaatgg gcgcaaaagc cgctctggtt gctcctgacg
     8941 aaaccacctt taactatgtc aaaggccgtc tgcatgcgcc gaaaggcaaa gatttcgacg
     9001 acgccgttgc ctactggaaa accctgcaaa ccgacgaagg cgcaactttc gataccgttg
     9061 tcactctgca agcagaagaa atttcaccgc aggtcacctg gggcaccaat cccggccagg
     9121 tgatttctgt gaacgacaat attcccgatc cggcttcgtt tgccgatccg gttgaacgtg
     9181 cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc gggtattccg ctgaccgaag
     9241 tggctatcga caaagtgttt atcggttcct gtaccaactc tcgcattgaa gatttacgcg
     9301 cggcagcgga gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca ctggtggttc
     9361 ccggctctgg cccggtaaaa gcccaggcgg aagcggaagg tctggataaa atatttattg
     9421 aagccggttt tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg aacaacgacc
     9481 gtctgaatcc gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa ggccgccagg
     9541 ggcgcggcgg gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct gctgtgaccg
     9601 gacatttcgc cgacattcgc aacattaaat aaggagcaca ccatggcaga gaaatttatc
     9661 aaacacacag gcctggtggt tccgctggat gccgccaatg tcgataccga tgcaatcatc
     9721 ccgaaacagt ttttgcagaa agtgacccgt acgggttttg gcgcgcatct gtttaacgac
     9781 tggcgttttc tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct gaacttcccg
     9841 cagtatcagg gcgcttccat tttgctggca cgagaaaact tcggctgtgg ctcctcgcgt
     9901 gagcacgcgc cctgggcatt gaccgactac ggttttaaag tggtgattgc gccgagtttt
     9961 gctgacatct tctacggcaa tagctttaac aaccagctgc tgccggtgaa attaagcgat
    10021 gcagaagtgg acgaactgtt tgcgctggtg aaagccaatc cggggatcca tttcgacgtg
    10081 gatctggaag cgcaagaggt gaaagcggga gagaaaacct atcgctttac catcgatgcc
    10141 ttccgccgcc actgcatgat gaatggtctg gacagcatcg gcctgaccct gcagcacgac
    10201 gacgctattg cgtcttatga agcaaaacaa cctgcgttta tgaattagac cccttgcccg
    10261 gtcaaatgac cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa cctttgcggt
    10321 taaaaataat gcgacaacag aaataatcgc aattacccag tagacagcaa agtgccccca
    10381 actttgtgca attgctccct gaataacacc agccagaatt accccggtag agatagtgtt
    10441 ggtaaataag gtggtagctg cccccgctct tccaggcatt aaatcctgaa accatagcat
    10501 cccaataccc gcaacaatgc cgataaatac agcgttaaaa agttgcagcg tcatcaacgc
    10561 catacggcta tgaaagaaaa tcaatccagt gtaaaacagc actcctgcca ccactgcaat
    10621 gaccatcatt cgccgcttac cataacgttt gacatagtag ccagccagaa tcattgctgg
    10681 tatttccagt ccagctgccg tccccatcag gaaacccgcc agtttgtctg gcaatcctaa
    10741 atcgctactg atccacaatg gcatatcaat aatgtacata gtgttgcagg tccacattaa
    10801 cgtcgaggcg acaaataaca tccgtacgtt actatcctgc cagtcacctt gcattgataa
    10861 agcattttcc gacggcagtt ctacccgcgc cacagacaga agcataaatg caatcaatac
    10921 cagactgagt gtgaatatcc cggcggcaat cgaaaacatc actgtaaagc cgtaattcaa
    10981 cgccagcata aaggccagcg gtggaccgat tacccatgcc agagaaagct gcgcacgcat
    11041 caccgagcta aacatcacca cttctcgcgc cgagttatcc gcgtattccc gcgccagagc
    11101 aaataactgt ggcattgccg tattggccag agatgccaga agcacaccac aggtgataag
    11161 cgtcagataa tgacgattaa atgcaaacag tagcgcattg ccaatagcca tcaaacagca
    11221 aaatataatc agttttcgcc gatcgccctg actgtcagaa cgttttgcca accagaggct
    11281 tacgccgatc ccagcaatag cattcaccgt ataaaagagt ccgatccaga aaggttgcgc
    11341 gccaacctca cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc cggccacccc
    11401 catcataaaa gcgaacagca taaatgccgc gtaaacaccg ttcatacggc gagccatcgt
    11461 cattatccag atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa ccagcaggta
    11521 taatctgctg gcgggtgatt ttacaccaat actcagtcac acatgatgca ggcaagtcaa
    11581 ctttcagaat tgcggtcatc ccactgcatc agtccttcaa gcatggttaa tcgttgtggg
    11641 acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg ctgataaaac
    11701 tgacgcatgg ggcacctcct tgcttcatcg ttaggaatta ttgacttaat atagggaaaa
    11761 taaaattgct gtcttttgcg caggagttcc ccttttatgc catctgctcg tctgcaacaa
    11821 cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc aggacacaac gctcaacgaa
    11881 ctggcagcgt tattgagctg ctcgcgtcgt catatgcgta ccctgctcaa caccatgcag
    11941 gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgcg gtaaacgctc gcgtctgaca
    12001 ttcctctata ccgggctggc gcttcagcaa cagcgggcgg aagacctgct ggagcaggat
    12061 cgtatcgacc aactagtgca gttggttggc gacaaagcga ctgtgcggca aatgctggtt
    12121 tctcatctgg gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct ctactatcgt
    12181 ccgttgcgta atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca tattgcccgg
    12241 caaatcttca gttcgctaac gcgcataaat gaggaaaatg gggaactgga agcagacatc
    12301 gcccaccact ggcagcaaat atcaccgctt cactggcgtt tctttttgcg tccaggagtc
    12361 cattttcatc atggtcgtga actggaaatg gacgacgtga tcgcctcttt aaaacgaatc
    12421 aatacgctgc cgctctattc gcatattacg gacattgtgt cgccgacgcc ctggacgctg
    12481 gatatccatc tcacgcagcc ggaccgctgg ttgccgttac tgctggggca agttccggcg
    12541 atgatcctgc cgtgcgaatg ggaaaccctc agtaactttg ccagccatcc tattggcacc
    12601 ggtccgtatg cggtgattcg caacagcacc aatcaactga aaattcaggc attcgatgac
    12661 ttcttcggtt accgggcatt aatcgacgaa gttaacgtct gggttctgcc ggaaattgcc
    12721 gacgagccag ccggtggtct gatgctaaaa ggtccacagg gcgagaaaaa agagattgaa
    12781 agccgcctgg aggaaggttg ctactattta ctgttcgaca gccgcaccca tcgcggggcg
    12841 aatcagcaag tcagggactg ggtaagctat gtgctttctc caactaatct ggtctatttc
    12901 gctgaggaac agtaccagca actgtggttc ccggcttatg gactgctccc ccgttggcac
    12961 catgcccgca ccataaagag cgaaaaaccg gctggcctgg aaagcctcac cctgaccttt
    13021 tatcaggatc acagtgagca tcgggtgatt gccgggatca tgcagcagat tctggcaagt
    13081 caccaggtca cgctggaaat caaagagatc agctacgatc agtggcatga aggagagatc
    13141 gagagcgata tctggcttaa tagcgccaac tttacgctgc cgctggattt ttcgctgttc
    13201 gcgcacttgt gcgaggtgcc gctgctccaa cactgtattc cgatcgactg gcaagccgac
    13261 gccgcccgct ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca actggtcgcc
    13321 agtaaagcaa tggtgccgct tatccaccac tggctgatca ttcagggaca acgcagtatg
    13381 cgcggcctgc gtatgaatac cctcggctgg ttcgatttta aatcagcgtg gtttgcgcca
    13441 ccggatccat gattgctggt agataacaaa atcactacac taacgccgtt ctcaacgggg
    13501 tgccacgcaa acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga taacgccggc
    13561 gaagggattt gaggctcctt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt
    13621 aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc gttttcgcta aacccgttct
    13681 gactgtttat acctacgatt ccttcgccgc cgactggggg cctggtccaa agatcaaaaa
    13741 agcttttgaa gccaactgta actgcgaact gaaactggtg gcgctggaag atggcgtttc
    13801 gcttctcaac cgtctacgga tggaaggcaa aaatagtaaa gccgatgtgg tgctggggct
    13861 ggataacaat ctgttagacg ccgccagtaa aaccggacta tttgccaaaa gcggtgtggc
    13921 agcggatgcc gttaacgttc ccggcggctg gaataatgac actttcgtcc cgtttgatta
    13981 cggctatttc gccttcgtct atgacaagaa caaactgaaa aacccgccgc aaagcctgaa
    14041 agaattggtt gagagcgatc agaactggcg ggtgatttat caggatccgc gtaccagcac
    14101 gccggggctg ggtctgctgt tgtggatgca aaaagtctat ggcgatgacg ccccacaagc
    14161 ctggcaaaaa ctggcgaaga aaaccgtcac ggtcaccaaa ggctggagcg aagcctacgg
    14221 cctgttttta aaaggtgaaa gcgatctggt actgagttac accacctctc cggcttatca
    14281 cattctcgaa gagaaaaaag ataactacgc cgccgcgaac tttagcgaag gtcactatct
    14341 gcaggtggaa gtcgccgccc gcaccgctgc cagcaagcag ccggagctgg cgcaaaaatt
    14401 cctccagttt atggtttctc cggcttttca gaatgcgatc ccaaccggta actggatgta
    14461 tccggtggca aatgtcacgc tgcctgccgg gtttgaacaa ttgaccaaac cggccaccac
    14521 gctggagttc acaccagctg aagtggcggc acaacgtcag gcatggatta gcgaatggca
    14581 acgcgccgtc agccgttaat tcccggctgg ttaattccag gtgtaagcgc cgccacgctg
    14641 gtggtagcgg ttgcgctcgc ggcgtttctc gccctgtggt ggaacgcgcc gcagggtaac
    14701 tgggtggcag tctggcagga cagctacctg tggcatgtgg tgcgcttctc cttctggcag
    14761 gcgtttctct cggcgctact ctctgtcgta cccgcgatat tcctcgcccg cgccctctat
    14821 cgcaggcgct ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac cttgatcctc
    14881 ccggtgctag ttgctgtttt cggcattctt agcgtctatg gtcgtcaggg ctggctggca
    14941 tcgctctgcc aatcgctcgg tctggagtgg accttttcgc cctacggcct gcaaggcatt
    15001 ttgctggcgc acgtattttt taatctgccg atggcgagcc gcttattact ccaggcactg
    15061 gaaaacattc ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg cggctggcat
    15121 tttttccgct tcgtcgaatg gccgtggcta cggcgacaaa tcccgccggt tgccgcgctt
    15181 atctttatgc tctgtttcgc cagcttcgcc accgtgctat cgctgggcgg cggtccgcag
    15241 gcaaccacta tcgagctggc tatttatcag gcgctgagtt acgactacga tcctgcccgc
    15301 gcggcgatgc tggcgctgct ccagatggtg tgttgccttg ggctggtgct gttgagtcag
    15361 cgactaagta aggccattgc gcccggcacc acgctgctgc aaggctggcg cgatccggac
    15421 gatcgtctgc atagccgcat ttgcgacacg gtgttaatag tgctggcgct gctgctgttg
    15481 ctgccaccgt tgctggcggt gatcgtcgat ggggtaaatc gccagttgcc ggaagtgctg
    15541 gcacaaccgg tgctgtggca ggcgctgtgg acctcgttgc gtattgcgct ggcggcaggt
    15601 gtattgtgcg tagtgctgac catgatgctg ctatggagca gtcgcgaact gcgggcgcgg
    15661 cagaaaatgc tggcgggtca ggcgctggag atgagcggca tgttgatcct cgccatgccg
    15721 gggattgtgc tggctaccgg cttcttttta ctgctcaaca acactatcgg cctgccacaa
    15781 tctgctgacg gcattgtgat tttcaccaat gcgttaatgg cgatccctta tgcgctgaaa
    15841 gtactggaaa acccgatgcg cgatatcacc gcccgctaca gtatgttgtg tcagtcgctg
    15901 ggcattgaag gctggtcgcg cttaaaagtg gtcgagctgc gcgccctgaa acgtccactg
    15961 gcgcaggcgc tggcttttgc ctgcgtgctg tcgattggtg attttggcgt agtggcgttg
    16021 ttcggtaacg atgatttccg taccctgccg ttttatctct accagcaaat tggctcctat
    16081 cgcagccagg acggcgcggt caccgcgtta attctgctac tactctgttt tctgctgttt
    16141 accgtgattg aaaaactacc ggggcgaaat gttaaaactg actgatatca cctggcttta
    16201 tcaccatttg ccgatgcgtt ttagcttaac ggtagaacgc ggcgagcagg tggcaatcct
    16261 cgggccaagc ggcgcgggta aaagtaccct gctgaatttg attgccggtt ttctgacgcc
    16321 agccagcggt tcgctgacta tcgatggtgt tgatcacacc acgacgccgc cgtcacgccg
    16381 tccggtgtcg atgctgtttc aggagaacaa cctgttcagc catctgacgg tcgcgcagaa
    16441 catcgggctg gggctaaatc cggggttgaa actgaacgcg gcacagcagg agaaaatgca
    16501 cgcgattgcc cgtcagatgg ggattgataa tttaatggcg cggttaccgg gcgagctttc
    16561 cggcggtcag cgacagcgcg tggcgttagc gcgttgtctg gtgcgcgaac agccgatttt
    16621 attgctcgat gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga tgctgacgct
    16681 ggtgagcacg agctgccagc agcaaaaaat gacgctattg atggtgtcgc acagtgtgga
    16741 agatgcggcg cggatcgcca cacgctcggt ggtggtcgcc gacgggcgca tcgcctggca
    16801 gggtaagact gaagaattgt tgagcggtaa ggcgagtgct tcggcaatat tggggattac
    16861 gggttagtat gcactgtcag atgcggcgtg aacaccttat ccgacctacg gacagtgcgt
    16921 tttgtaggcc tgataagacg cggtcagcgt cgcatgaggc aacccgcaca agacaatccc
    16981 ctaaacccca accactttac gcaaaatatc gatatacacc ggcatcaacg ggtggcgaat
    17041 taacaccacc agcgccacca cgccgatggc agaaatcaac ggcgtcagcc acaacaaacg
    17101 gccgcgcgac aaataatgac ttaaacgatc ggttgcttta ccgctgcgcc ataaccgcca
    17161 gcacagccag ccaccaaccc acaaaaatac cgccgtcgcc agcagcaacc atttaaactc
    17221 gccgctctgc attccggctg gaatatcgat cgccgcgccc gccagaatcc caggcaggaa
    17281 gtaaaacggt ggccacagca ggcagccgat aatattcggc gtaatgaatt ttgccaccgg
    17341 cagatccagc attcccgcca ccattggcac cagcggacgc gtcgggccaa caaagcgacc
    17401 gaccagaatg gtgaacatgc tgtgttgatg caacgcatgt tcagttttat cgagtaatgc
    17461 tttgtttttc ttcagaaatg accagcgatg caacggcttt ttaaaacgcc agcccagcca
    17521 gaaggagatc cagtcgccca gcagacaacc gacaatccct gccagccaag cgtgccagaa
    17581 acttaactcg ccgctgccaa tcagcgctcc cagccccgcc atcagcaccg taccgggtag
    17641 aatcaaaccg accagcgcca gcgactccag aaaggccacc aacaccaccg ccatcaatga
    17701 atacacggta gattgggtaa taaagtgttc cagcaatgct tgcataatgt gcctgtcaaa
    17761 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
    17821 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
    17881 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
    17941 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
    18001 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
    18061 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
    18121 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacacgat
    18181 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
    18241 caagcagatt catcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
    18301 tttgcccaaa caggtcgctg aaatacggct ggtgcgcttc atccgggcga aagaaacccg
    18361 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
    18421 aaacccactg gtgataccat tcgcgagcct ccggatgaca accgtagtga tgaatctctc
    18481 ctggcgggaa cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca
    18541 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
    18601 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
    18661 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
    18721 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
    18781 tcttttactg gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt
    18841 aacaaagcag gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
    18901 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
    18961 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
    19021 acccgttttt ttggatggag tgaaacgatg gcgattgcaa ttggcctcga ttttggcagt
    19081 gattctgtgc gagctttggc ggtggactgc gccagcggtg aagagatcgc caccagcgta
    19141 gagtggtatc cccgttggca aaaagggcaa ttttgtgatg ccccgaataa ccagttccgt
    19201 catcatccgc gtgactacat tgagtcaatg gaagcggcac tgaaaaccgt gcttgcagag
    19261 cttagcgtcg aacagcgcgc agctgtggtc gggattggcg ttgacagtac cggctcgacg
    19321 cccgcaccga ttgatgccga cggtaacgtg ctggcgctgc gcccggagtt tgccgaaaac
    19381 ccgaacgcga tgttcgtatt gtggaaagac cacactgcgg ttgaagaagc ggaagagatt
    19441 acccgtttgt gccacgcgcc gggcaacgtt gactactccc gctatattgg cggtatttat
    19501 tccagcgaat ggttctgggc aaaaatcctg catgtgactc gccaggacag cgccgtggcg
    19561 caatctgccg catcgtggat tgagctgtgc gactgggtgc cagctctgct ttccggtacc
    19621 acccgcccgc aggatattcg tcgcggacgt tgcagcgccg ggcataaatc tctgtggcac
    19681 gaaagctggg gcggcttgcc gccagccagt ttctttgatg agctggaccc gatcctcaat
    19741 cgccatttgc cttccccgct gttcactgac acctggactg ccgatattcc ggtgggcacc
    19801 ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat ttccggcggc
    19861 gcgtttgact gccatatggg cgcagttggc gcaggcgcac agcctaacgc actggtaaaa
    19921 gttatcggta cttccacctg cgacattctg attgccgaca aacagagcgt tggcgagcgg
    19981 gcagttaaag gtatttgcgg tcaggttgat ggcagcgtgg tgcctggatt tatcggtctg
    20041 gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt ttggtcgcgt actcggctgg
    20101 ccgctggaac agcttgccgc ccagcatccg gaactgaaag cgcaaatcaa cgccagccag
    20161 aaacaactgc ttccggcgct gaccgaagca tgggccaaaa atccgtctct ggatcacctg
    20221 ccggtggtgc tcgactggtt taacggtcgt cgcacgccaa acgctaacca acgcctgaaa
    20281 ggggtgatta ccgatcttaa cctcgctacc gacactccgc tgctgttcgg cggtttgatt
    20341 gctgccaccg cctttggcgc acgcgcaatc atggagtgct ttaccgatca ggggatcgcc
    20401 gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa accaggtcat tatgcaggcc
    20461 tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg ctgtgcgctc
    20521 ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc atcagcccag
    20581 caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgc gcagcgaaca ggcacaacgc
    20641 tttgaacagc tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca acactatctt
    20701 ccaacttccg ccccggcaca ggctgcccag gccgttgcga ctctataagg acacgataat
    20761 ggcgattttt gataattatg aagtgtggtt tgtcattggc agccagcatc tgtatggccc
    20821 ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc tgaatacgga
    20881 agcgaaactg ccctacaaac tggtgttgaa accgctgggc accacgccgg atgaaatcac
    20941 cgctatttgc cgcgacgcga attacgacga tcgttgcgct ggtctggtgg tgtggctgca
    21001 caccttctcc ccggccaaaa tgtggatcaa cggcctgacc atgctcaaca aaccgttgct
    21061 gcaattccac acccagttca acgcggcgct gccgtgggac agtatcgata tggactttat
    21121 gaacctgaac cagactgcac atggcggtcg cgagttcggc ttcattggcg cgcgtatgcg
    21181 tcagcaacat gccgtcgtta ccggtcactg gcaggataaa caagcccatg agcgtatcgg
    21241 ctcctggatg cgtcaggcgg tctctaaaca ggatacccgt catctgaaag tctgccgatt
    21301 tggcgataac atgcgtgaag tggcggtcac cgatggcgat aaagttgccg cacagatcaa
    21361 gttcggtttc tccgtcaata cctgggcggt tggctatctg gtgcaggtgg tgaactccat
    21421 cagcgacggc gatgttaacg cgctggtcga tgagtacgaa agctgctaca ccatgacgcc
    21481 tgccacacaa atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc gtattgagct
    21541 ggggatgaag cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca cctttgaaga
    21601 tttgcacggt ctgaaacagc ttccaggtct gcccgtacag cgtctgatgc agcagggtta
    21661 cggctttgcg ggcgaaggcg actggaaaac cgccgccctg cttcgcatca tgaaggtgat
    21721 gtcaaccggt ctgcagggcg gcacctcctt tatggaggac tacacctatc acttcgagaa
    21781 aggtaatgac ctggtgctcg gctcccatat gctggaagtc tgcccgtcga ttgccgtaga
    21841 agagaaaccg atcctcgacg ttcagcatct cggtattggt ggtaaggacg atcctgcccg
    21901 actgatcttc aatacccaaa ccggtccagc gattgtcgcc agcctgattg atctcggcga
    21961 tcgttaccgt ctgctggtta actgtatcga cacggtgaaa acaccgcact ccctgccgaa
    22021 actgccggtg gcgaatgcac tgtggaaagc gcaaccggat ctgccaactg cttccgaagc
    22081 gtggatcctc gctggtggcg cgcaccatac cgtcttcagc catgcgctga acctcaacga
    22141 tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg gtgattgata acgacacccg
    22201 cctgccagcg tttaaagacg cactgcgctg gaacgaagtg tattacggat ttcgtcgcta
    22261 agtagccaca tcaggtatgt aacgcctgat gcgacgctga cgcgtcttat ctggcctaca
    22321 cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg cattcaacgc ctgatgcgac
    22381 gctggcgcgt cttatcaggc ctacgcgctg cgattttgta gaccggataa gcgaagcgca
    22441 tccggcacga aggagtcaac atgttaaaag atctcaaacg ccaggtatta gaagccaacc
    22501 tggcgctgcc aaaacacaac ctggtcacgc tcacatgggg caacgtcagc gccgttgatc
    22561 gcgagcgcgg cgtctttgtg atcaaacctt ccggcgtcga ttacagcgtc atgaccgctg
    22621 acgatatggt cgtggttagc atcgaaaccg gtgaagtggt tgaaggtacg aaaaagccct
    22681 cctccgacac gccaactcac cggctgctct atcaggcatt cccctccatt ggcggcattg
    22741 tgcatacgca ctcgcgccac gccaccatct gggcgcaggc gggtcagtcg attccagcaa
    22801 ccggcaccac ccacgccgac tatttctacg gcaccattcc ctgcacccgc aaaatgaccg
    22861 acgcagaaat caacggcgaa tatgagtggg aaaccggtaa cgtcatcgta gaaacctttg
    22921 aaaaacaggg tatcgatgca gcgcaaatgc ccggcgttct ggtccattcc cacggcccgt
    22981 ttgcatgggg caaaaatgcc gaagatgcgg tgcataacgc catcgtgctg gaagaggtcg
    23041 cttatatggg gatattctgc cgtcagttag cgccgcagtt accggatatg cagcaaacgc
    23101 tgctggataa acactatctg cgtaagcatg gcgcgaaggc atattacggg cagtaatgac
    23161 tgtataaaac cacagccaat caaacgaaac caggctatac tcaagcctgg ttttttgatg
    23221 gattttcagc gtggcgcagg caggttttat cttaacccga cactggcggg acaccccgca
    23281 agggacagaa gtctccttct ggctggcgac ggacaacggg ccgttgcagg ttacgcttgc
    23341 accgcaagag tccgtggcgt ttattcacgc cgatcaggtt ccccgcgctc agcatatttt
    23401 gcagggtgaa caaggctttc gtctgacacc gctggcgtta aaggattttc accgccagcc
    23461 ggtgtatggc ctttactgtc gcgcccatcg ccaattgatg aattacgaaa agcgtctgcg
    23521 tgaaggtggc gttaccgtct acgaggccga tgtgcgcccg ccagaacgct atctgatgga
    23581 gcggtttatc acctcaccgg tgtgggtcga gggtgatatg cacaatggca ctatcgttaa
    23641 tgcccgtctg aaaccgcatc ccgactatcg tccgccgctc aagtgggttt ctatagatat
    23701 tgaaaccacc cgccacggtg agctgtactg catcggcctg gaaggctgcg ggcagcgcat
    23761 cgtttatatg ctggggccgg agaatggcga cgcctcctcg cttgatttcg aactggaata
    23821 cgtcgccagc cgcccgctgc tgctggaaaa actcaacgcc tggtttgcaa accacgatcc
    23881 cgatgtgatc atcggctgga acgtggtgca gttcgatctg cgaatgctgc aaaaacatgc
    23941 cgagcgttac cgtcttccgc tgcgtcttgg gcgcgataat agcgagctgg agtggcgcga
    24001 gcacggcttt aaaaacggcg tcttttttgc ccaggctaaa ggtcggctaa ttatcgacgg
    24061 tatcgaggcg ctgaaatccg cgttctggaa tttctcttca ttctcgctgg aaactgtcgc
    24121 tcaggagcta ttaggcgaag gaaaatctat cgataacccg tgggatcgaa tggacgaaat
    24181 tgaccgccgt ttcgccgaag ataaacctgc gctggcaact tataacctga aagattgcga
    24241 gctggtgacg cagatcttcc acaaaactga aatcatgcca tttttactcg aacgagcaac
    24301 ggtgaacggc ctgccggtgg atcggcacgg cggttcggtg gcagcgtttg gtcatctcta
    24361 ttttccgcga atgcatcgcg ctggttatgt cgcgcctaat ctcggcgaag tgccgccgca
    24421 cgccagccct ggcggctacg tgatggattc acggccaggg ctttatgatt cagtgctggt
    24481 gctggactat aaaagcctgt acccgtcgat catccgcacc tttctgattg atcccgtcgg
    24541 gctggttgaa ggcatggcgc aacctgatcc agagcacagt accgaaggtt ttctcgatgc
    24601 ctggttctca cgggagaaac attgcctgcc ggagattgtg actaacatct ggcacgggcg
    24661 cgataaagcc aaacgtcagg gtaataagcc gctttcgcag gcgctgaaga tcattatgaa
    24721 tgccttttat ggcgtgctcg gcaccaccgc ctgccgcttc ttcgatccgc gcctggcatc
    24781 gtcgatcacc atgcgtggtc atcagatcat gcggcagacc aaagcattga ttgaagcgca
    24841 gggctacgac attatctacg gagataccga ctcgactttt gtctggctaa aaggtgcaca
    24901 ttcggaagaa gaggcggcga aaatcggtcg tgcactggtg cagcacgtta acgcctggtg
    24961 ggcggaaacg ctgcaaaaac aacggctgac cagcgcatta gaactggagt atgaaactca
    25021 tttctgccgt tttcttatgc caaccattcg cggagctgat accggcagta aaaagcgcta
    25081 cgccgggctg attcaggagg gcgacaagca gcggatggtg tttaaagggc tggaaaccgt
    25141 gcgcaccgac tggacgccgc tggcccagca gtttcagcag gagctgtacc tgcgtatctt
    25201 ccgcaacgag ccatatcagg agtacgtccg ccaaaccatc gacaaactga tggcgggtga
    25261 actggatgcg cggctggttt accgtaaacg ccttcgccgt ccgctgagcg aatatcagcg
    25321 taatgttccg cctcatgtac gcgccgctcg ccttgccgat gaagaaaatc aaaagcgcgg
    25381 tcgccccttg caatatcaga atcgcggcac cattaagtac gtatggacca ccaacggccc
    25441 ggagccgctg gactaccaac gttcaccact ggattacgaa cactatctga cccgccagct
    25501 acaacccgtg gcggagggaa tactcccttt tattgaggat aattttgcta cacttatgac
    25561 cgggcaactt gggctatttt gagcaaaaaa aagagttcgc cagataccat tttgatgcgt
    25621 gacgaatgct ttgccatcca gtaccatagc gccctttcca ttcctggacc tgaataacac
    25681 cactacctca taagcacggt agcgggtggt tattgcctgc aattaaagat atagagccga
    25741 acacatatgc cttttacact tggtcaacgc tggatcagcg atacagaaag cgaattggga
    25801 cttggaaccg ttgtcgcggt ggatgcgcga actgtcactt tacttttccc atctactggt
    25861 gaaaaccgtc tgtacgcacg cagtgattcc cccgtgaccc gcgtgatgtt caaccctggt
    25921 gataccatta ccagccatga cggctggcag atgcaagtcg aagaagtaaa agaagaaaat
    25981 ggcttgctga cctatatcgg tactcgcctg gatactgaag agtccggcgt agccctgcgt
    26041 gaagttttcc ttgatagcaa actggtgttc agcaaaccgc aggaccgtct gtttgccggg
    26101 cagattgacc gtatggaccg ctttgcgctg cgttatcgcg cgcgtaagta ttccagcgaa
    26161 cagttccgta tgccgtacag cggcctgcgc ggtcagcgta ccagcctgat cccgcatcag
    26221 ctcaacatcg ctcatgatgt tggtcgccgc cacgcgccgc gcgtcctgct ggctgacgaa
    26281 gtgggtttag ggaaaaccat tgaagccggg atgatcctgc atcagcaact gctctctggc
    26341 gctgctgaac gtgtgctgat tatcgtcccg gaaaccttac agcatcagtg gctggtagaa
    26401 atgctgcgcc gtttcaacct gcgctttgct ctgtttgatg atgagcgtta tgccgaagct
    26461 cagcacgatg cttacaaccc gtttgacacc gaacagctgg tgatttgctc gctggatttt
    26521 gcccgtcgta gcaaacagcg cttggaacat ctctgtgaag ccgaatggga cctgctggtg
    26581 gtcgatgaag cgcatcacct ggtgtggagc gaagatgcgc caagccgtga atatcaggcc
    26641 attgaacaac tggcagagca cgtgccgggc gttctgctgc tgaccgcgac cccggaacag
    26701 ctggggatgg aaagccactt cgcccgtctg cgtctgctgg acccgaaccg tttccacgat
    26761 tttgcgcagt tcgttgaaga gcagaaaaat tatcgtccgg ttgcggacgc cgttgccatg
    26821 ctgctggcag gtaacaaact gagcaatgac gaactgaaca tgctcggcga gatgatcggc
    26881 gagcaggata tcgagccgct gttgcaggca gcaaacagcg acagcgaaga tgcccagagc
    26941 gcccgtcagg agctggtttc gatgctgatg gatcgccacg gcaccagccg cgtgctgttc
    27001 cgtaacacgc gtaacggtgt gaaaggattc ccgaaacgcg agctgcacac cattaagctg
    27061 ccgctaccga cgcagtatca gacggctatt aaagtctccg gcattatggg cgcacgtaaa
    27121 agtgcggaag atcgtgctcg cgatatgctc tacccggagc gtatttatca ggaatttgaa
    27181 ggtgataacg ccacctggtg gaacttcgat ccgcgcgttg agtggctgat gggctacctg
    27241 accagccatc gctctcagaa agtgctggtg atctgcgcca aagctgccac tgcgctgcaa
    27301 ctggagcagg tactgcgcga acgtgaaggt attcgcgctg cggtgttcca cgaaggtatg
    27361 tcgattatcg aacgtgaccg cgctgccgcc tggtttgccg aagaagacac cggcgcacag
    27421 gtactgctgt gctcagaaat cggttctgaa ggacgtaact ttcagttcgc cagtcacatg
    27481 gtgatgtttg acctgccatt caacccggat ctactggagc agcgtattgg tcgtctggat
    27541 cgtatcggcc aggcgcacga tattcagatc catgtgcctt atctggagaa aaccgctcag
    27601 tcggtgctgg tgcgctggta tcacgaaggt ctggatgcat ttgagcacac ctgcccgacc
    27661 ggacgcacta tttacgatag cgtatacaac gatctgatta actatctggc ttcaccggat
    27721 caaaccgaag gctttgacga tctgatcaaa agctgccgcg agcaacatga agcgctgaaa
    27781 gcacagctgg aacagggtcg tgaccgcctg ctggaaatcc actccaacgg tggcgaaaaa
    27841 gcccaggcac tggcagaaag cattgaagag caggatgacg ataccaacct gatcgccttc
    27901 gccatgaacc tgttcgatat tatcggtatc aatcaggacg atcgcggcga caacatgatc
    27961 gtgctgacgc cgtccgatca tatgctggtg ccggacttcc ctggcctgtc ggaagatggc
    28021 atcaccatca cctttgatcg tgaagtggcg ctggcgcgtg aagatgcaca gtttattacc
    28081 tgggagcatc cgctgatccg caacggtctg gatctgatcc tttctggcga taccggtagc
    28141 agcacgattt cactgttaaa aaacaaagcg ttgccggtag gtacgctgtt ggtggaactg
    28201 atttatgtgg ttgaagccca ggctccgaag cagttgcagc tcaaccgctt cctgccaccg
    28261 acgccggtac gtatgctgct ggataaaaac ggcaacaacc tggcggcgca ggtagagttt
    28321 gaaaccttta accgccagct taacgcggtt aaccgtcaca ccggcagcaa actggttaac
    28381 gccgtgcagc aggatgttca cgctatcctt caactgggtg aagcgcagat cgagaaatct
    28441 gcccgtgcat tgattgatgc agcgcgtaac gaagccgacg aaaaactgtc tgccgagctg
    28501 tctcgtctgg aagctctgcg tgcagtgaac ccgaacattc gtgacgacga actgaccgcc
    28561 attgagagca accgtcagca ggtaatggaa agcctggatc aggcaggttg gcgtctggat
    28621 gccctgcgtt tgatcgttgt aacgcatcag taacggagcc gaaaatgggg atggaaaact
    28681 acaatccacc gcaggaaccc tggttggtta tcctgtatca ggatgaccat attatggtgg
    28741 tcaacaagcc gagcggtttg ttgtcagtgc cgggtcgtct ggaagagcac aaagacagcg
    28801 tgatgacgcg cattcagcgt gattatccgc aggcagaatc ggtgcatcgt ctggatatgg
    28861 ctaccagcgg cgtgattgta gtggcgctga ccaaagccgc ggagcgggag ttaaaacgcc
    28921 agttccgcga gcgcgagccg aaaaagcagt atgtggcccg cgtctggggg catccatccc
    28981 ccgcagaagg tctggtggat ctgccgctga tttgcgactg gccaaaccgc ccgaaacaga
    29041 aagtctgtta cgaaacgggt aaacctgcgc agacggaata tgaagtggtg gagtatgcgg
    29101 cggataacac ggcaagagtg gtgttaaaac cgattaccgg gcgttcgcat caattgcgtg
    29161 tgcatatgct ggcgctgggg catccgattc tcggcgatcg tttttatgca tcaccagaag
    29221 cgagagcgat ggcaccgcgt ttgttgctac atgcagagat gttgacgatt acccatccgg
    29281 cgtatggcaa tagtatgacg tttaaagcgc cagcggattt ttaatcgtca cgctggcgca
    29341 gttcggtagg cggataaggc gttcacgccg catccggcaa ttgtgcgccg attgcctgat
    29401 gcgacgcggg ccgcgtctta tcaggcctac agtttaaaac ttatttaaac cctttctgct
    29461 gctttatcag ctcatatgcc tgctgaattt cctgcgcttt ctgcttcgcc atctccatca
    29521 tctcaggcgg caaacctttc gccaccagct tatcgggatg gtgttcactc atcagcttac
    29581 ggtaggcacg tttgatggtg gtcgcatcat ccgtcggctt cacgcccagc acattacagg
    29641 catcttccag cgtcgggcca cgctgcgctt gctgccagtt accaccgcca gattgctgct
    29701 gataaccgcc gccaaactgt gcaccgccct gcatcatgcg caaaaactgg tcaaactggg
    29761 cgcgggagat ccctaattct tcagcaatga catacagcac cgcccgttca ttcgggtgca
    29821 gtgaaccatc agcaaacgcc gcctgaatct ggatctccag aaacatacga attaagtcaa
    29881 aacgaccaaa gcagacactg cgaaactggc gcatcttttc gcgcagcggg taattatctg
    29941 attttcccac ccggaacgcg ttttgcgccg cagtacggga agcgccatga aggttcattc
    30001 ggtccatcaa ctggctggcg atatgaatat cagcctccgt gacgcgacct ttggatttgg
    30061 ttaaatgccc catcacttca aaagtggtgg caaaaaacag cgcctgacgc tcacgctggt
    30121 tggcgaacca cgccatttta cggctacggg ctttatcaaa catatggcca attaacaggc
    30181 ctaacactac gccccaaaag ccgccgccca tcagtaaggc cacggccacg ccaatgattt
    30241 ttccccaata ctgcatatat tccccaaatc gacacacgga tatcagggct atctcccaca
    30301 atataaaggt gcttttaccg ttttccggct gcggtcaatt gtgggacatc gcctataatt
    30361 tgcattatca tacctgtcat tcaatgccgt gcctaacacc actgacgcta ttcggacagg
    30421 attaacacta gcgtagagat gacgagtacg ttagtctctg accgtttgtc acgcgcaacg
    30481 ttaccgatga tggaacaata aaatcaacgt atgaaaaaac gtatccccac tctcctggcc
    30541 accatgattg ccaccgccct ttatagtcaa cagggactgg cagccgacct cgcctcacag
    30601 tgcatgttgg gcgtgccaag ctatgaccgt cctctggtac agggcgatac caatgactta
    30661 cccgtgacta tcaatgctga ccacgcgaaa ggggactacc cggatgacgc cgtgtttact
    30721 ggcagcgtgg atatcatgca gggtaacagc cgtctgcagg ccgacgaagt gcagctccat
    30781 caaaaagagg caccaggaca accggagccg gtacgtaccg ttgatgcgct cggtaatgtc
    30841 cattacgacg ataaccaggt gatcctcaaa gggccgaaag gctgggcgaa tctgaacacc
    30901 aaagatacca acgtctggga aggtgattac cagatggtgg gtcgccaggg tcgcggtaaa
    30961 gcggacctga tgaaacaacg tggcgaaaac cgctatacca ttctggataa cggtagcttt
    31021 acctcctgtc tgccgggttc tgacacctgg agcgtggtag gtagcgaaat tattcatgac
    31081 cgcgaagaac aagttgctga gatctggaac gcccgcttta aggtgggtcc ggtaccgatc
    31141 ttttatagcc cctatttgca gttgccggtg ggtgacaaac gtcgctctgg tttcttgatc
    31201 ccgaacgcca agtacaccac caccaactac tttgagttct acctgccata ttactggaac
    31261 atcgcgccaa atatggatgc caccatcacg ccgcattata tgcatcgtcg tggcaacatc
    31321 atgtgggaga acgaattccg ctacctctcc caggcgggcg ctggcttgat ggaactggac
    31381 tatctgcctt cagataaagt ctatgaagat gaacacccga acgatgacag ttcacgtcgt
    31441 tggttattct actggaacca ctccggggtc atggatcagg tgtggcgttt caacgtcgac
    31501 tacaccaagg tcagcgatcc tagctacttc aatgatttcg ataacaagta cggttccagt
    31561 actgacggct acgcaacgca aaaattcagc gttggctatg cggtgcaaaa cttcaatgcc
    31621 accgtttcaa ccaagcagtt ccaggttttc agcgaacaga acaccagtag ctactcggca
    31681 gagccgcagt tagacgttaa ttactaccag aatgatgttg gtccgtttga tacgcgtatt
    31741 tacggccagg cagtgcactt tgttaacacc agagacgaca tgcctgaagc aacccgtgtt
    31801 cacctggaac cgaccatcaa tttgccgctc tctaataact ggggcagcat caataccgaa
    31861 gcgaagtttc tggcaaccca ttatcagcaa accaatcttg actggtataa ctccagaaac
    31921 acgaccaagc tggacgaatc cgttaaccgc gtaatgccgc aattcaaagt tgacggcaaa
    31981 atggtctttg aacgcgatat ggaaatgctg gctccgggtt atacccaaac gctggaaccg
    32041 cgcgcgcagt atttgtacgt gccgtatcgc gatcagagcg acatctataa ctacgactcg
    32101 tctctgctgc aatctgacta ctctggcctg ttccgggacc ggacttacgg cggtcttgac
    32161 cgtattgcct ccgctaacca agtgacgacc ggtgtcacat ctcgcatata tgatgatgct
    32221 gccgttgaac gttttaatat ttccgttggt caaatctact atttcacgga gtctcgcact
    32281 ggcgatgaca acataacatg ggagaatgac gacaaaacgg gctcactggt gtgggcaggc
    32341 gacacctact ggcgtatctc cgagcgttgg ggattgcgtg gcgggattca gtacgataca
    32401 cgtctggata acgtagcgac cagtaactcc agcattgaat accgtcggga tgaagaccgt
    32461 ctggtacagc tgaattacca ttacgccagc ccggaatata ttcaggctac gctgcctaag
    32521 tactattcca ctgctgagca atataagaat ggtatttcgc aggtaggtgc tgtcgccagc
    32581 aggccaattg ccgatcgttg gtccattgtt ggggcctact actacgacac caatgcgaac
    32641 aagcaagccg actctatgtt aggtgtgcaa tacagctcct gctgctatgc aattcgcgtc
    32701 ggttacgagc ggaagctgaa cggttgggat aacgataaac aacatgcggt atatgacaac
    32761 gcaatcggct ttaacatcga acttcgcggc ctgagctcca actacggtct gggtacgcaa
    32821 gagatgctgc gttcgaacat tctgccgtat caaaacactt tgtgatttgt tgatttacca
    32881 cgtaatccgc agtgcggtta attgaaatgg aaaaagtatg aagaactgga aaacgctgct
    32941 tctcggtatc gccatgatcg cgaataccag tttcgctgcc ccccaggtag tcgataaagt
    33001 cgcagccgtc gtcaataacg gcgtcgtgct ggaaagcgac gttgatggat taatgcagtc
    33061 ggtaaaactg aacgctgcgc aggcaaggca gcagcttcct gatgacgcga cgctgcgcca
    33121 ccaaatcatg gaacgtttga tcatggatca aatcatcctg cagatggggc agaaaatggg
    33181 agtgaaaatc tccgatgagc agctggatca ggcgattgct aacatcgcga aacagaacaa
    33241 catgacgctg gatcagatgc gcagccgtct ggcttacgat ggtctgaact acaacactta
    33301 tcgtaaccag atccgcaaag agatgattat ctctgaagtg cgtaacaacg aggtgcgtcg
    33361 tcgcatcacc atcctgccac aggaagtcga atccctggcg cagcaggtgg gtaaccaaaa
    33421 cgacgccagc actgagctga acctgagcca catcctgatc ccgctgccgg aaaacccgac
    33481 ctctgatcag gtgaacgaag cggaaagcca ggcgcgcgcc attgtcgatc aggcgcgtaa
    33541 cggcgctgat ttcggtaagc tggcgattgc tcattctgcc gaccagcagg cgctgaacgg
    33601 cggccagatg gggtggggcc gtattcagga gttgccgggg atcttcgccc aggcattaag
    33661 caccgcgaag aaaggcgaca ttgttggccc gattcgttcc ggcgttggct tccatattct
    33721 gaaagttaac gacctgcgcg gcgaaagcaa aaatatctcg gtgaccgaag ttcatgctcg
    33781 ccatattctg ctgaaaccgt cgccgatcat gactgacgaa caggcccgcg tgaaactgga
    33841 gcaaattgct gctgatatca agagtggtaa aacgactttt gctgccgcag cgaaagagtt
    33901 ctcgcaggat ccaggttctg ctaaccaggg cggtgatctc ggctgggcta caccagatat
    33961 tttcgatcca gccttccgtg acgccctgac ccgcctgaac aaaggtcaaa tgagtgcacc
    34021 ggttcactct tcattcggct ggcatttaat cgaactgctg gatacccgta atgtcgataa
    34081 aaccgacgct gcgcagaaag atcgtgcata ccgcatgctg atgaaccgta agttctcgga
    34141 agaagcagca agctggatgc aggaacaacg tgccagcgcc tacgttaaaa tcctgagcaa
    34201 ctaatggtta aaacccaacg tgttgtgatc actcccggcg agcccgccgg gattggcccg
    34261 gacttagttg tccagcttgc acagcgtgag tggccggtcg aactggttgt ttgtgccgat
    34321 gccactctcc ttaccgaccg ggcagcgatg ctcggtttgc cgctcaccct ccgcccttat
    34381 tcccccaact cccctgcaca accgcaaact acgggcacat taacgctact tcctgtcgcg
    34441 ctacgtgaat ctgtcactgc ggggcagtta gcgattgaaa atgggcatta cgtggtggag
    34501 acgctggcgc gagcgtgtga tggctgtctg aacggtgaat ttgctgcgct gatcacaggc
    34561 cccgtgcata aaggcgtcat taacgacgca agcattccgt ttaccggtca taccgagttt
    34621 ttcgaagagc gttcgcaggc gaaaaaagtg gtgatgatgc tggcgaccga agaacttcgc
    34681 gtggcgctgg caacgacgca tttaccgctg cgcgatatcg cagatgctat cacccctgca
    34741 cttttgcacg aagtgattgc tattttgcat cacgatttgc gtacaaaatt tggtattgcc
    34801 gaaccgcgca ttctggtctg cgggctgaat ccgcacgcgg gcgaaggcgg tcatatgggt
    34861 acggaagaga tagacaccat tattccggtg ctcgacgagc tgcgggcgca ggggatgaaa
    34921 ctcaacgggc cgctgcctgc cgataccctg tttcagccga aatatctcga taacgccgac
    34981 gccgtgctgg cgatgtacca cgatcagggt cttcccgtgc taaaatacca gggcttcggg
    35041 cgcggtgtga acattacgct gggcctgccc tttattcgca catcagtgga ccacggcacc
    35101 gcgcttgaac tggcgggacg tggcgaagcc gatgtcggca gttttattac ggcgcttaat
    35161 ctcgccatca aaatgattgt taacacccaa tgaataatcg agtccaccag ggccacttag
    35221 cccgtaaacg cttcgggcaa aactttctca acgatcagtt cgtgatcgac agcattgttt
    35281 ctgccattaa cccgcaaaag ggccaggcaa tggtcgaaat cggccccggt ctggcggcat
    35341 tgaccgagcc ggtcggcgaa cgtctggacc agctgacggt catcgaactt gaccgcgatc
    35401 tggcggcacg tctgcaaacg catccattct taggcccgaa actgacgatt tatcagcagg
    35461 atgcgatgac ctttaacttt ggcgaactgg ccgagaaaat gggtcagccg ctgcgtgttt
    35521 tcggcaacct gccttataac atctccacgc cgttgatgtt ccatctgttt agctatactg
    35581 atgccattgc cgacatgcac tttatgttgc aaaaagaggt ggtgaatcgt ctggttgcag
    35641 gaccgaacag caaagcgtat ggtcgattaa gcgtcatggc gcaatactat tgcaacgtga
    35701 tcccggtact ggaagttccg ccgtcagcct ttacaccacc acccaaagtg gattccgccg
    35761 tcgtgcgcct ggtccctcat gcaacgatgc ctcacccggt taaagatatt cgcgtgttga
    35821 gccgcatcac caccgaagcc tttaaccagc gtcgtaaaac cattcgtaac agcctcggca
    35881 acctgtttag cgtcgaggtg ttaaccggaa tggggatcga cccggcgatg cgagcggaaa
    35941 atatctctgt cgcgcaatat tgccagatgg cgaactatct ggcggagaac gcgcctttgc
    36001 aggagagtta acgatgatca attcgccccg agtgtgtatt caggttcaaa gcgtctacat
    36061 tgaggcacaa tcttcacctg ataatgaacg ttacgttttt gcttataccg taaccatacg
    36121 caatctgggg cgagcgccag tgcagttgtt ggggcgttac tggctgatca ccaatggcaa
    36181 tggccgtgaa accgaagtcc agggcgaagg agtggttggc gtccagccac ttatcgcgcc
    36241 tggcgaagag taccagtaca ccagcggtgc aatcattgaa accccgctgg gcaccatgca
    36301 gggtcactac gaaatgatcg atgaaaatgg cgtccctttc agcatcgaca ttcccgtatt
    36361 ccgactcgcc gttcccacac tcattcatta aaagaatatg gcgacatacc ttattggcga
    36421 cgttcatggt tgttacgatg aactgatcgc attgctgcat aaagtagaat ttacccccgg
    36481 gaaagatacc ctctggctga cgggcgatct ggtcgcgcgc ggcccgggtt cgctggatgt
    36541 tctgcgctat gtgaaatcct taggcgacag cgtacgtctg gtgctgggca atcacgatct
    36601 gcatctgctg gcggtatttg ccgggatcag ccgcaataaa ccgaaagatc gcctgacacc
    36661 gctgctggaa gcgccggatg ccgacgagct gcttaactgg ctgcgtcgcc agcctctgct
    36721 gcaaatcgac gaagagaaaa agctggtgat ggcccacgca gggatcacgc cgcagtggga
    36781 tctgcagacc gccaaagagt gcgcgcgcga tgtagaagcg gtgctgtcga gtgactccta
    36841 tcccttcttt cttgacgcta tgtacggcga tatgccaaat aactggtcac cggaattgcg
    36901 ggggctggga agactgcgtt ttatcaccaa cgcctttacc cgtatgcgtt tttgcttccc
    36961 gaacggtcaa ctggatatgt acagcaaaga atcgccggaa gaagcccctg ccccactgaa
    37021 accgtggttt gcgattcctg gccctgtcgc tgaagaatac agcatcgcct ttggtcactg
    37081 ggcatcgctg gaaggcaaag gtacgccgga aggtatttac gcgctggata ccggctgctg
    37141 ctggggtggg tcattaacct gcctgcgctg ggaagataaa cagtattttg tccagccgtc
    37201 gaaccggcat aaggatttgg gcgaagcggc ggcgtcttaa acacagcctg atacaggaag
    37261 gccggataag acgcgaccgg cgtcgcatcc ggcactagcc gtaaattcta tacaaactta
    37321 ccgccgctcc agaatctcaa agcaatagct gtgagagttc tgcgcatcgg cgtcgtggaa
    37381 ttcgctgaat accgattccc agtcatccgg ctcgtaatcc gggaaatggg tgtcgccttc
    37441 cacttctgcg tcgatatgcg tcagatacag tttttgcgct tttggcagga actgctcata
    37501 aacgcgaccg ccgccaatca ccatgatttc tggtacgtca ccacacgccg caatggcttc
    37561 atccaccgat ttcacccacg ttacgcgatc gtccgtaccc ggttgactgc tgaggataat
    37621 atttttgcgt cctggcaacg gacgaccgat tgattcccaa gtatggcggc ccataatcac
    37681 gggtttattt aaggtgttgc gtttaaacca ggcgagatcg gcaggcaggt tccacggcat
    37741 ggcgttttcc atgccgataa cgcgatctac cgctaacgcc gcaatcagac tgatcattga
    37801 gatttcccga taaaaaaatt gtcgccacta tacgtaaagc gtaaaccgtc gtcgactggt
    37861 gcgaggatga tgttgaggaa aattttatat tctgctggcg agtccacgct ctccccctgg
    37921 actcgccgca ttacaatgaa acaaaaacaa acagttagct gtaaagtgtg atttacgtca
    37981 ctctttatta agatgagggt ttcgtttccg gttcatccgc catgttgccg gtatgtttac
    38041 cttcttctgt tccctgccag ccatgtcgtt gaattaatga cagatgttcg cggtcctcgg
    38101 tgatgatctc acttaacatc gcgctggtgc gtttatagac cgccgcgcgg gctttggtgt
    38161 cattctcaac cattgccatc tcttccacca tctgaatatt aaagcggcgg aacacatcgg
    38221 cacgttctcg cgcttcatac ggccccagac ctaaactttc cagcgccaga cgcccggttt
    38281 tcagcgcacc ttcaaaggtt tcacgctccg gcttttcaac gcctgcctga cgcaaacgaa
    38341 tgtagtgatc gacatcgcgg gcgcgggcaa taatctgcaa atgcgggaaa tgttctttca
    38401 ccatctccgt cagttgcagg ttggtttgcg gatcgtcaat agcgttaatc agcacttccg
    38461 ctttcgccgc tccggcagat tccagtaaat ccatccgcgt ggcatcgcca taaaacactt
    38521 tcataccaaa tttacgcagc gtttcgatat ggtccggatc gtgatcgagc accaccattt
    38581 tcaccccgct ggagagcagt aaacgtccgg taatctgtcc aaaacgaccg aatccggcga
    38641 taatcacgcg cggctgttct tcatcgatct catcggcttc acgcgcctcc tccgtagaag
    38701 attgctcaag gcgattgagg atcaccagca gaatcggcgt tgctgccatc gacagcgcca
    38761 ccgccagggt cagcgatttc gcccactccg gctccagcac attcgccatc tgcgccgcac
    38821 caaataccac aaaggcaaac tcactgccct gccctaacaa caccgcaaac cagcgacgct
    38881 gtttatttgg cacctgcaac ggtcgggcaa tcagccacag catggcgatt ttgatgatga
    38941 ggaaaccgag cagcaaaatg acaatgcgca atgggttttc aatcagcgtg ccaaagtcta
    39001 tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa acctttaaac ggttcgatat
    39061 cgctttccaa cgcatgacgg tattccgagc ttgccagcag tacgcccgcc agaaacgcgc
    39121 ccatcgccat cgacaagccg acctcttcca gcagcaaacc aaagccaaac acaaggaata
    39181 acgccacggc actaaacact tcccgcaagc cagagcgggc aacaaaacgc agcgccggac
    39241 gcgtaacata gcgccccagc aataccacca gtaccagcgc acccgccact tttaacgccg
    39301 agagagcaaa tgcgcccatc gtcgtcgatg cactgctcgc cgccagtagc gggatcatcg
    39361 ccaccagcgg gattgccgcg atatcctgga acagcagcac tgcaaaggca ctgcggccca
    39421 tttgcgtcac catcagattg cgttcattca tcgcctgcat ggcaatcgcc gttgatgaga
    39481 gcgccagcgt cataccgatc aattccgcga cctgccagcg cagcccaagt aacatacaga
    39541 acagccccag caggccaccg caaatcacca tctgcaatgc gccaccgccg aacactgccg
    39601 cacgcagctt ccacagcctt tgtggatcga gttcgaggcc gataataaac agcatcagca
    39661 ccaccccaat ctcggcaaag tgcagaatag attcggcatc ggtcaccagt cgcagccccc
    39721 acgggccaat aatgcagccg gcgatcaggt agccaagtac cgatcccaga ccaagacgta
    39781 ccgcaatggg tacaatcagc gccgccgatc cgaggtagat cagcgcctga accagcgtat
    39841 gactatccat gatgggcctc ctgccattcc agcagacgtt gcttatagtg gcgcgcctgc
    39901 ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca tggcaaacgg tggcagccag
    39961 ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg acagcacatc aaagcccgga
    40021 tgcgcaccaa tttcaaaatg gctttccccg ccgccggtcg tcaccgccca cagcaaatgt
    40081 ttgccatgca gcgccgtgcc gccgtgaccg taagcccagc cgtgcgacaa aactttatcg
    40141 atccaaagtt taagaagcgg aggaatgctg taccactgca tcgggtgctg ccagacgatc
    40201 agatcggcgc gagagagcgc ctcctgctcg gcggcaatat cgatattgaa gtcaggatag
    40261 agttgataaa gagagcgaat ttcgacgcct tccagcgtcc ttgcctgttc aagcatccgt
    40321 ttattcgcat gggaatgatg cggatacgga tgcgcataaa tgatgaggat catagttatt
    40381 agcccgcgtg tttacgcctt tgttttccca caaagtgtag tcatatattc acgcggctta
    40441 tagtcaataa tattgattca gaaaagagag aaatctgaac aaataatccc attctctgcc
    40501 cgcggatcgt catattgctg gtctgcgcca gtgacattcc tcgagtctcc ggggcaaacg
    40561 ctacggaaat caacaagcca aacagcgaga tacccgcccc cattagcatc gtgttactga
    40621 taccgtaatt attgataaat atcggcagtg cccaggtcga aacaatggtg ccaatacgac
    40681 ttaaggacat aatcacgccc acggcagagg cgcggatatc cgtcgggaag agttcattag
    40741 gatagagcca ctgcaaatta cccggcccgc cagagaaaaa ggcatacacc gcaaaggcca
    40801 tcactaccag ccagatcccc atatccggga ttagccccaa aaccgccaac gccagcgtca
    40861 tcatggcaaa gctgccaatc aacaatggac gccgtccggc agtgtttagc cacagcatcg
    40921 gcggaataca gccgagcata aagaacaggc taatcaccac attccctagt gccgcgtttt
    40981 tgccaacccc caatcccaac gaaccaacga tttgcgggcc aaaggtgtaa atggcgaaca
    41041 ttgggatcac ctggcaggtc cagatggcgg caacaaacag aacaaaagga aaatggcggc
    41101 gattaaacag atcgcgaaaa cgggtttgct gcggctgctc ttcatcgaaa gccaccggtt
    41161 cgccaaacag ttttatcatc atctcttcgc actcttttac tcgccctttg cgtaataacc
    41221 agcggggaga ttctggcagt tcgaatcgac caatcaaaat caacaaacaa gggatcgccg
    41281 cgctacccag catccagcgc caaccacctt ccacatcata aagccagtag ccgaccagat
    41341 cggcgcaggt cgcgccgaca taccacatcg cggcaataaa gctgatggaa aacgcccgct
    41401 gacgggtact ggagaactcg gtgatcatcg aagtggcgat gggataatct gcaccgatga
    41461 caatgccgat gagtacccgc atcaccaaca gttcgacggg ggatgaaaca cacatcgtcg
    41521 ccaccgatat cacgccgatg gcgatgatat caatgaggaa cattttgcgc cgtccgactt
    41581 tatcggagat atagccaaac agcgatgtgc caacgaacag cccggcgagc gttcccgcgc
    41641 ccagcaagcc aatccagtca gcgtccagtt tcagcgccgg agtcagttgc tccagcgcca
    41701 cgccaatcat taccagtaca taaggaccac cgcttcccca cagcaaaatg cggcggtgaa
    41761 tagaggagaa tttgagatcg tcaaagtttc tggacggttg catgggttat tcctgttttt
    41821 ttgcaatgag agtcagtacg gcgttacgta tcaaaccaac atcagccgta acggaactcc
    41881 acaccaaagg tgccgcgcgg gtattcccac tgttccagcg ccgaccccag ccccagaatg
    41941 cgacaggtgc cgcactccag acatccggcg taatcgaagc gcacactgcc gtcatcctgc
    42001 ttcttgtaca gacctgcggg gcacgctttc accagcagct ccagcacctg tttatcagca
    42061 tcggccttca caacaatgtg cggatgctct tcatcgacat tgaatttatt gacgcccagt
    42121 ttgacgtcca cattgacggg agaagtcata aaacggtcac tcctttcatg ccatccttga
    42181 tcagattgat gaagcccact ttcttgccgt ggcggaggat tttcttgcgc atcagttccg
    42241 gcgcactgcc gtcaatggtg aacaggtcac gcgccacgcc caccgccagt tccgggttgc
    42301 cgctaaacat gcgtgggtta tcaaggaacg ccggtagttt ctggtacatg cgcatatcgc
    42361 gcagcgggcc actctccaga tgctgacgat attccgcgag tttttgctta ctgaaatcgt
    42421 cgcttttcat cgctgaaagc acggtttttg ctgcagcttc cccggcggca atcgccagat
    42481 ccataccgcg aatggtaaaa ccgaggttca tacacattcc ggcggcatca ccggcaatca
    42541 atacgccgtc accgaccagc tccggcagca tgttgatgcc tgcttccggc actacgtgag
    42601 cggaatattc caccagcttg ccgcccgcga tcagcggtgc aacggccgga tgctgtttga
    42661 aatcttccag catttgcggc accgattttt tcgcgtcatg cagatgatgc agaccacaaa
    42721 ccagccccag cgacagggta ttttcattgg tataaaggaa gccgccgccc atcaggccat
    42781 cggtgggtga tcccgcaaac agacaagccg cgccctgatt accctgcaac tgaaaacggt
    42841 cttcgattac cgacttcggt aactcgatca gttccttcac gccaaccgcc acatccgtcg
    42901 gtttaacgcg ttttgccatc cccaactttt cggcaaggat ggagttcacc ccatcagcaa
    42961 ggagcaccgt tttcgcttca atcacatcac catcggcttc tacaccgacg actttgccat
    43021 cgcgctgtac gaggttatcg acgcggatcc cggtaattaa ctgcgcgccc gcttcttcgg
    43081 cctgctccat caaccaggca tcaaatttac tgcgcaaaac ggagtaagaa cgctgggatg
    43141 gcgaggtttc gtcaccgttg cagtagtcca tagtcatcgc tgacttttcc gtcataaacg
    43201 cgagtttttc atgggtgatc aggcgttcta cgggggcgga atcggcgaaa cctggaataa
    43261 tgtgttccag actgtgggca tagagacgcc cgccagtaac gttcttggca cctgcggaat
    43321 tgccacgctc gataactaac acttgcgccc cttcgcgggc gagcaccagt gcggcaaccg
    43381 aaccggcaag ccctgcaccg acgatgatgg cgtcaaagat atcttcggac ataagagctc
    43441 cctgtcagcg gcagagccgt aatgcagccc tgccagagtg gatcaacgcg ctaaagctgc
    43501 ggtcagtgcc ggaaggatct tcacggcatc gccaacaatg ccgtaatccg cgtactggaa
    43561 gatcggcgca tttttatctt tattgatggc gaaaatggtt tgcgacgcgt tagcgccaac
    43621 catgtgctgg atctacccgg agatccccac cgccaggtac agttcaggtt tcagcatcag
    43681 gttggagata ccgacatagc gttcgtgctc catccatttt tcgttttctg ccaccggacg
    43741 agaacaggcc aactccgcac ctatcgcctt gcaaagctgt tctgccagcg caatgttctc
    43801 tttgctgcca atgccgcgac cgacgctgac caccagacgg gctttgtcga gatcgacgct
    43861 gttgctctgg cgcgcctggg ttgccgtgcg ggtaatcgcc acagccggag cctgccactc
    43921 cacggtgtgc gtttcgccag tgcgtgacgc gtctggctga gccgcatcga acgtgccgct
    43981 gctgatggtc agtaccgcat acggcgtggc aatgcgttct tcgccaatcg ccagaccgcc
    44041 gtaaaccatg tgtttcactg ttgctttacc gtcctgtacg ctgacggtgc tggcatcgtt
    44101 agacaccgcc gctttaaggc gataacccag ttttgccgcc agtaatttgc cgcgacgggt
    44161 gtttggcaac agcaccagac cgtcagcgcc gtgctggcga atagtgtcag ccatgacacc
    44221 ggcgtaatct tcgatcatcc gatcgtccgg tttgccgttt aatttccaga catgattagc
    44281 gccgagctgg attgcctgtg cgccgtcggc atcattgagg acaaaggcat tgatttgatt
    44341 agctaaagcc tgcgcaccgt tcatcagttc cggcagacga gagggggtat cgctgaatac
    44401 ccagacttga gaaaacgtgt tcatagcatc ccctgtaatt aaatgacttt gcgaagattt
    44461 tcagcaaatg cggcgatctg ttcttcgccg tcgccttcaa tcacgatgcg ctgacgctcg
    44521 cgctgtttcg gcgcggcaac ctgttgttca gaccaggcgt ccacggcgtt aaaaccaata
    44581 tccgccgccg accatacctg gactggcttt ttcgccgcgc cgagaatggc tttcatcgaa
    44641 ggaatttgtg gggagttgat atcagtggaa acagcaacaa ccgcaggcag cggaatgctt
    44701 aaggtttcgg tttcatcttc cagttcgcgc tcaacggtga gggtatctgc cgtcagggag
    44761 ataattttgc tgacgccgtt aactgccgga atattgagga tttcgcccac cagcagacca
    44821 acctgctggg cataaaggtc ggaagaacca tcgccacaga ggatcagatc aaagcctgct
    44881 ttctgggcgg ctgcagccag tgcgctcgcc gtttgttgcg gcagtgcctg ctcgaactgg
    44941 tcatcaatca ccacaatcag ttcatccggg ccgcgcgata gcacatcttt acgccctttg
    45001 gcgttggtca gggctttacc gcccacactt aaggctgtca cctgcgcctc tgctgcctgt
    45061 tgctttagct ggcaagccgc ttcaatagcg ttgagatcgt attggcttat tttggcatcg
    45121 gctttgctga agtctaatga accatcagca ttattgaccg caatatcctg ttcatcaggc
    45181 acgcacttat agcaagtaat aatcttcatt gcatctccag aaatcatgaa ggaaacgtcg
    45241 ttcatatcgg tggtgttaat tacagatatc acgcccttta atttggaaat aaaactttaa
    45301 tcaccaatat tgaaagtcag atgctatttt tttacccaca aaagaatata acaaactgat
    45361 taataaccaa aattaacgaa aaacgccctc tgttcactcg aaacaaaaat gtgataccaa
    45421 tcacagaata cagcttattg aatacccatt atgagttagc cattaacgcg tccacgaggt
    45481 taataataat tatattaaat gttaacaaaa ataaaacaaa cgggaacgca aaataaatat
    45541 tcgttttcac agtggaatta actcatgaag aatgaaaaga gaaaaacggg aatagaaccg
    45601 aaggttttct ttccgccatt aataatcgtc ggcatacttt gttggcttac agtcagagat
    45661 ctggatgcgg cgaatgtcgt tattaatgct gtattcagtt acgtcaccaa tgtatgggga
    45721 tgggcatttg aatggtatat ggtggtgatg cttttcggtt ggttctggct ggtgtttggc
    45781 ccgtatgcca aaaagcgttt aggtaacgaa ccgccagaat ttagcaccgc cagttggatc
    45841 tttatgatgt tcgcctcctg tacgtctgct gccgtactgt tctggggatc gattgagatc
    45901 tactactaca tctccacccc gccgtttggc ttagaaccga actcgacagg agcgaaagag
    45961 ttggggctgg cttacagctt gttccactgg ggacctctgc cgtgggccac ttacagcttc
    46021 ctttcagtcg ccttcgctta cttcttcttt gtccgcaaaa tggaagtgat tcgccccagc
    46081 tcgacactgg tgccgctggc aggtgaaaaa cacgccaaag ggttgttcgg taccatcgtc
    46141 gacaacttct atctcgtcgc cttgatcttc gcgatgggta ccagtctggg ccttgccacg
    46201 ccgctggtga ccgagtgtat gcaatggttg tttggcattc cgcataccct gcaactggac
    46261 gctatcatca ttacctgctg gattatcctc aacgccattt gcgtcgcttg cggtctgcaa
    46321 aaaggggtac gtatcgccag tgacgtgcgt agttacctga gcttcctgat gctgggttgg
    46381 gtgttcatcg tcagcggtgc cagcttcatc atgaactact tcaccgattc ggtggggatg
    46441 ttgctgatgt atctgccgcg catgttgttc tataccgatc ccatcgctaa aggcggcttc
    46501 ccgcagggct ggaccgtgtt ctactgggca tggtgggtga tttacgccat ccagatgagt
    46561 atcttcctcg cccgcatctc ccgtggtcgt actgtgcgtg aactgtgctt cggcatggtg
    46621 atggggctga cagcatccac ctggatcctg tggaccgtgc tcggtagtaa cactctgctg
    46681 ttgatggata aaaacatcat caacattcca aatctgatcg aacagtacgg tgtggcgcgc
    46741 gccatcatcg aaacctgggc cgctctgcca ctcagcaccg ccaccatgtg gggcttcttc
    46801 atcctctgct ttattgccac cgttacgctg gttaacgcct gctcttatac cctggcgatg
    46861 tctacttgcc gcgaagtacg cgatggtgaa gaaccaccgc tgctggtgcg tatcggttgg
    46921 tcaattctgg ttggcattat cggtattgtt ctgctggcgc tcggcggcct gaaaccgatt
    46981 caaaccgcca ttatcgccgg aggatgcccg ctgttcttcg tcaacattat ggtgacgctc
    47041 tcctttatta aagacgcgaa acagaactgg aaagattaat taacccccaa aatatcaaga
    47101 gattgaaaga tggattttaa tttaaatgat gagcaggaac tgtttgtcgc cggtatccgc
    47161 gaactgatgg ccagcgaaaa ctgggaggcc tattttgccg agtgcgaccg tgacagcgtc
    47221 tacccggaac gttttgtcaa agcactggcg gatatgggta tcgacagtct gctgatccct
    47281 gaagagcacg gtggtctgga cgcggggttt gttactctcg ccgccgtgtg gatggagctg
    47341 ggacgtctgg gggcaccaac ctatgtactg taccagctgc cgggcgggtt caataccttc
    47401 ctgcgcgaag gcacacaaga gcagatcgac aagattatgg ctttccgcgg caccggtaag
    47461 cagatgtgga actcagcgat taccgaaccg ggcgcgggct ccgacgtggg tagcctgaaa
    47521 acgacttata cccgtagaaa tggtaagatt tatcttaatg gtagtaagtg ttttattacc
    47581 agtagcgcct acaccccgta catcgtggtg atggcgcgcg acggggcttc tccggacaaa
    47641 cctgtctaca ccgaatggtt tgttgatatg agcaaaccgg gcatcaaagt gaccaaactt
    47701 gagaagctcg gtctgcgtat ggatagctgc tgtgaaatca cctttgacga tgtggaactg
    47761 gacgagaaag acatgttcgg tcgggaaggt aacggcttta accgcgtcaa agaagagttc
    47821 gaccatgaac gtttcctggt agccctgacc aactacggta cggcgatgtg cgcctttgaa
    47881 gatgcggcgc gctacgccaa ccagcgcgtg cagtttggcg aggctattgg tcgtttccag
    47941 ttgattcagg aaaaattcgc ccacatggcg atcaaattaa actccatgaa aaacatgctg
    48001 tatgaagcag cgtggaaagc agacaacggc accatcacct ctggcgatgc agcgatgtgc
    48061 aaatacttct gcgccaatgc ggcatttgaa gtggtggata gcgcaatgca ggtgctgggc
    48121 ggtgtcggga ttgcgggcaa ccaccgcatc agccgcttct ggcgtgacct gcgtgtagac
    48181 cgcgtttccg gaggatctga cgaaatgcag atcctgacgc tgggtcgtgc ggtgctgaag
    48241 caataccgct aagtccgtgc gctgcctgat gcgacgctga cgcgtcttat caggcctacg
    48301 ggagcacgaa tgtaagccgg ataaggcgtt tacgccgcat ccggcagtca tgcgctacat
    48361 aaatttttca ggagctaata atggatcatc tacccatgcc gaaattcgga ccgctggccg
    48421 gattgcgcgt tgtcttctcc ggtatcgaaa tcgccggacc gtttgccgga caaatgttcg
    48481 cagaatgggg cgcggaagtt atttggatcg agaacgtcgc ctgggccgac accattcgcg
    48541 ttcaaccgaa ctacccgcag ctctcccgcc gcaatttgca cgcgctgtcg ttaaatattt
    48601 tcaaagatga aggccgcgaa gcgtttctga aattaatgga aaccaccgat atcttcatcg
    48661 aagccagtaa aggtccggcc tttgcccgtc gtggcattac cgatgaagta ctgtggcagc
    48721 acaacccgaa actggttatc gctcacctgt ccggttttgg tcagtacggc accgaggagt
    48781 acaccaatct tccggcctat aacaccatcg cccaggcctt cagtggttac ctgattcaga
    48841 acggtgatgt tgaccagcca atgcctgcct tcccgtatac cgccgattac ttttctggcc
    48901 tgaccgccac cacggcggcg ctggcagcac tgcataaagt gcgtgaaacc ggtaaaggcg
    48961 aaagtatcga catcgccatg tatgaagtga tgctgcgtat gggccagtac ttcatgatgg
    49021 attacttcaa cggcggcgaa atgtgcccgc gcatgagcaa aggtaaagat ccctactacg
    49081 ccggttgcgg tctgtataaa tgtgccgacg gctacatcgt gatggaactg gtgggcatta
    49141 cccaaattga agagtgcttt aaagatattg gcctcgcaca tctactcggt acgccggaaa
    49201 tcccggaagg cactcagctt atccaccgta tcgaatgccc ttacggccca ctggtagaag
    49261 aaaaactcga tgcctggctg gcggcacata ccatcgcaga agttaaagaa cgctttgccg
    49321 aactgaatat cgcctgtgcc aaagtactga ccgtaccgga actggaaagc aatccacagt
    49381 atgttgcccg tgaatcaatc actcagtggc aaacgatgga tggtcgcacc tgcaaagggc
    49441 cgaacatcat gccgaaattc aaaaataacc ccggacaaat ctggcgcgga atgccctcac
    49501 atggcatgga cacggctgcc attttgaaaa atatcggcta cagcgaaaac gacattcagg
    49561 agttggtcag caaaggtctg gccaaagttg aggactaaac gcgtggcgtc agtcggcttg
    49621 cctgactagc gcccgcccaa taacaaacga acaaaaatgg atagaggtgc aatggatatc
    49681 attggcggac aacatctacg tcaaatgtgg gacgatctgg cggacgttta cggtcataaa
    49741 acggcgctga tttgtgaatc cagcggcgga gtcgttaacc ggtatagtta tcttgagtta
    49801 aatcaggaga ttaaccgcac ggcaaacctg ttttatacgc tggggattcg caaaggcaac
    49861 aaggttgcac tacatctcga caactgcccg gaatttatct tttgctggtt cgggctggca
    49921 aaaattggcg cgattatggt gccgattaac gcccgcctgt tacgcgagga aagcgagtgg
    49981 atcttgcaaa atagccaggc gtgcctgctg gtgaccagtg cgcaatttta tcctatgtat
    50041 caacagattc agcaggaaga tgccactcaa ttgcggcaca tttgcctgac agatgtggca
    50101 cttcccgctg atgatggagt gagttcattt actcaactga aaaatcaaca acctgccacc
    50161 ttgtgctatg caccgccgct atcgactgac gatacggcgg aaattctctt cacctccggc
    50221 accacctccc gaccgaaagg tgtggtgatt acccattaca acctgcgctt cgctggatat
    50281 tactccgcct ggcagtgtgc actgcgtgac gatgacgtct acatgacggt aatgcctgcg
    50341 tttcatatcg attgccagtg tactgcggcg atggcggcgt tttctgccgg ggccaccttt
    50401 gtgctggtcg agaaatacag cgcccgcgcc ttctggggac aggcgcagaa gtaccgcgcc
    50461 accattaccg aatgtattcc gatgatgatc cgtacgttga tggtgcagcc gccttcagcg
    50521 aacgatcggc aacaccgcct gcgggaagtg atgttttatc tcaacttgtc ggagcaggaa
    50581 aaagacgcat tttgtgaacg cttcggcgtt cgcttgctga cgtcttatgg gatgacggaa
    50641 accattgtgg gcattatcgg cgatcgccct agcgataaac gacgctggcc gtcgattggt
    50701 cgggcggggt tttgctacga agcggagatc cgcgacgatc acaatcgccc gctcccggca
    50761 ggtgagatcg gtgaaatctg tattaaaggc gtaccaggga aaaccatctt caaagagtat
    50821 tttctcaacc cgaaagccac tgcgaaagtg ctggaagccg acggctggct acataccggc
    50881 gataccggat accgcgacga agaaggcttt ttttatttcg tcgatcgccg ctgcaatatg
    50941 atcaaacgcg gtggcgagaa tgtctcctgc gtggagctgg aaaatatcat cgccacccat
    51001 ccgaaaattc aggacatcgt ggttgtgggt attaaagatt cgattcgcga tgaagccatc
    51061 aaagcatttg tggtgctgaa tgaaggtgaa acattgagcg aagaggaatt ttttcgcttc
    51121 tgcgaacaaa atatggcgaa atttaaagtg ccctcttatc tggagatcag aaaagatctg
    51181 ccacgtaatt gctcggggaa aataattaga aagaatctga aataaatgaa acgtcaggga
    51241 actaccctgc cagctaataa tcacgcccta aaacaatatg cattttttgc agggatgttg
    51301 tcgtccctaa aaaagcaaaa atggagaaaa ggaatgagtg aatcattaca tctgacccgc
    51361 aatggatcaa ttctggaaat tacccttgat cgcccaaaag cgaatgctat tgatgcaaaa
    51421 accagctttg aaatgggcga agtatttcta aatttccgtg acgatccgca attacgtgtc
    51481 gccattatta ccggtgccgg agagaaattc ttttccgcgg gctgggattt aaaagcggca
    51541 gcagaaggcg aagcaccgga tgctgacttt ggcccgggtg gttttgcggg attaaccgaa
    51601 attttcaatc tcgacaaacc agttatcgca gctgtgaacg gctacgcctt tggcggcggt
    51661 ttcgaactgg cgctggcggc agattttatt gtttgtgccg ataacgccag cttcgccctg
    51721 ccggaagcca aactgggcat cgttcctgac agcggcggtg tgctgcgtct gccgaagatc
    51781 ctgccgccta ccatcgtcaa tgaaatggtg atgaccggca gacgaatggg cgcagaagag
    51841 gcgctgcgtt gggggatagt caaccgcgtg gttagccagg cggaactgat ggataacgcc
    51901 cgcgaactgg ctcagcagct ggttaacagc gccccgctgg cgattgcggc gctgaaagag
    51961 atctaccgca ccaccagcga aatgccggta gaagaagcgt atcgctatat tcgcagcggc
    52021 gtgttgaaac actatccatc ggttctgcat tcggaagatg ccattgaagg gccgctggcg
    52081 tttgccgaga agcgcgatcc ggtgtggaaa ggacgttaac aaccgtgagc tattacgcct
    52141 ttgaggggtt aattccggtg gttcacccga cggcgtttgt ccatcccagt gccgtcttga
    52201 ttggcgatgt gattgtggga gccggtgtct acatcggccc actcgcctca ctgcgtggtg
    52261 actacgggcg gttgatcgtg caagcgggag ccaatattca ggatggctgc attatgcatg
    52321 gctactgcga cactgacacc atcgttgggg aaaacggcca tatcgggcat ggagcgatcc
    52381 tgcatggttg tgtgattggt cgcgatgcat tggtcgggat gaacagcgta attatggatg
    52441 gcgcggtcat tggcgaagag agcattgttg ccgcaatgag ctttgtcaaa gcaggcttta
    52501 gtggcgagaa acgccagttg ttgatgggta cgccagcccg cgctgtacgc agtgttagtg
    52561 acgacgagtt acactggaaa cggttgaata ccaaagagta tcaggatctt gtcgggcgct
    52621 gtcatgcatc gttacatgaa acgcagccac tgaggcaaat ggaggaaaat cgcccccgtt
    52681 tgcaggggac gacggatgtg acgcctaaac ggtgatcttg ccggatgcga cctggttgct
    52741 ggataagatg ctacaacatc gtatctggca agcaggacgc ccggtatcaa cgagataaaa
    52801 ttaacgacgc atactctttg acaacatcat ctgccacttc tgctcgcggt taagttccgt
    52861 gagcggaacg gcaggaatac gcggtgtctt tcttacgccg actaattttt cccgactgtt
    52921 attgcgcagg cgcgcgtaaa tttgctcatc gatatcgacc actttaacca gtcgctgaca
    52981 ctggcatccc cgcccttcca gcttattagg gatcatctta acttcgcagc ttatttctgt
    53041 gacttccgac agaatataag tcagggtatt aaccgccttg ctgtgttcaa tatcgaaatg
    53101 aatagagatc tctcttgcta tcacccaccg attttcagcc atcatccatt cggcgattaa
    53161 caagtagagt ggtttttcaa catatccttc acacatacga actccagttt cacaaattaa
    53221 aagtctagct atgactcgtt ttgaagcaat taaacaaggc catattaaaa ttgtggatat
    53281 atcgatagta tgcaacttca ccgtcgataa gtgtgagcta aatcctgctt atgtcatcaa
    53341 aaatatcgat tccccaaaag atttacttaa tgggcaaaaa aaaacggtcc tcatcagaga
    53401 accgtattag ttattcgatc aaattagcgg atgaaaaata tccgccacga cacgctatta
    53461 tttgatctgt gcgtgcattt cctgcaccga aattactttt tcagtcgcat cggcattcag
    53521 cgccatcgcg gtagcgaaac caccgttcag ggtggtgtca taatgcactt tatattgcag
    53581 cgcactgcga cggatcacgc gggagtcttc aatcgcacga cggcctgagg tggtgttgat
    53641 gatgtaggta tattcgccat tcttgatacg gtcctgaatg tgcggacggc cttcatgcac
    53701 cttgtttacc agacgcggat tgatacccgc ttcgcccagc acaatcgccg tgccgtgggt
    53761 cgcatccagc tcgaagccct gtttcagcag ttttgccgcc aggtccacca cgcgttcttt
    53821 atcgccttcg cgcacggaaa gcagcgcacg accgtgtttc ttcatggtgg agttgctgcc
    53881 cagctgcgct ttggcgaacg cttcagcgaa ggtgcggccc acgcccatga cttccccggt
    53941 agagcgcatt tctggcccta acagcgggtc aacgcctggg aatttattga acggcagcac
    54001 cacttctttc accgagtagt acggcggaat aacttctttg gttacgccct gctcagccag
    54061 cgatttgcca gccatcacac gcgccgccac ttttgccagc ggtacgccgg tggctttgga
    54121 gacgaacgga acagtacgcg ccgcacgcgg gtttacttca atcaggtaaa cttcgttgtt
    54181 tttcaccgca aactgcacgt tcatcagacc gcgcacctgc aattcgaagg ccagtttctg
    54241 cacctgctgg cgcatcacat cctgaatttc ctgacttaag gtgtacgctg gcagcgaaca
    54301 tgcggagtca ccggagtgca cacccgcctg ctcgatgtgc tccatgatgc cgccaatcag
    54361 caccatttcg ccgtcgcaga tggcatccac gtcaacttct actgcgtcat caaggaaatg
    54421 atccagcaac actggcgcat cgttagacac gctaaccgcc gtctggaagt agcgacgcag
    54481 gtcagcttcg tcatagacga tttccatcgc ccgaccgccg agaacgtaag acggacgcac
    54541 caccagcggg tagccaatct cttttgcctt ctcaactgcc atttcaatag cagtaacggt
    54601 ggcgttcgcc ggttgtttca gtttcagacg gtcaaccgca tgctggaagc gttcacggtc
    54661 ttccgcacgg tcgatagcat ccgggctggt gccgataacc ggtacgccag cggcttccag
    54721 tgcacgcgcc agtttcagcg gggtctgacc gccgtactga acgataacgc ctttcggctt
    54781 ctcaatacgc acgatttcca gcacatcttc cagagttacc ggctcgaagt agaggcgatc
    54841 ggaagtgtcg tagtcggtag agacggtttc cgggttacag ttaaccataa tggtttcgta
    54901 accgtcttca cgcagcgcca gcgaggcgtg tacgcagcag tagtcgaatt cgataccctg
    54961 accgatacgg tttggaccgc cgccaagcac catgattttt tcacggtcgg tagacggatt
    55021 cgcttcgcac tcttcttcat aagtggagta catgtaagcg gtgtcggtgg caaactctgc
    55081 cgcacaggta tccacgcgct tgtagaccgg gtgcaggtca tattggtcac gcagcttacg
    55141 gatttccgct tcgcgtacgc ccgccagttt tgccaagcgc gcatcggcaa agcctttgcg
    55201 tttcagctgg cgcaggaatt cagcgttcag gccagtgatg cccacttccg ccactttctc
    55261 ttccagacgc accagttctt caatctgtac caggaaccag cggtcaatgt tagtcaggtt
    55321 gaagacgccg tccacagaca ggcccgcgcg gaaagcatcg gcgatgtacc agatacgctc
    55381 tgcgcctgcg tctttcagtt cgcgacggat tttggttaac gcttccgggt catccaggct
    55441 cactttcggg tcgaagccag ttgcgcccac ttccaggccg cgcagcgctt tttgcaggga
    55501 ttcctgctgc gtgcgaccaa tcgccatcac ttcgccaacc gatttcatct gagtggtcag
    55561 acggtcgtta gcaccggcga atttttcgaa gttgaagcga ggaattttag taaccacata
    55621 gtcgatggac ggctcgaagg aggccggagt acgtccgcca gtgatgtcgt tcatcagttc
    55681 gtctagggtg taacccaccg ccagtttcgc cgccacttta gcaatcggga aaccggtcgc
    55741 tttcgacgcc agcgccgaag aacgggacac gcgtgggttc atttcgataa caatcaggcg
    55801 accgtttttc gggttcaccg caaactggac attggaacca ccggtttcaa cgccgatttc
    55861 acgcagcacc gccatcgagg cgttacgcat gatttgatat tctttgtcgg tcagcgtttg
    55921 ggctggcgcg acagtgatgg agtcaccggt gtggatgccc atcgcatcaa agttttcgat
    55981 agagcagacg atgatgcagt tgtcgttttt atcacgcacc acttccatct cgtactcttt
    56041 ccagccgatc agcgactcat caatcagcaa ctctttggtc ggagagagat ccagaccgcg
    56101 ggcgcaaatt tcttcaaact cttcacggtt ataagcgata ccgccgccgc taccgcccat
    56161 ggtaaaggat gggcgaataa tgcacgggaa gcccacgtca gcggcaacag ccagcgcttc
    56221 ttccatcgtg tgtgcgatac cggaacgcgc ggtttccaga ccaattttct tcatcgctac
    56281 gtcgaaacga cggcggtctt ctgctttatc aatcgcatcg gcagtggcac caatcatggt
    56341 gacaccgaac tcttccaaca cgccctgacg ttccagctcc agcgcgcagt tcagcgccgt
    56401 ctgaccgccc atggttggca gcaccgcgtc cgggcgctct ttttcaataa tcttgcgtac
    56461 cacttcccag tgaatcggct cgatgtaggt cgcatcggcc atttccgggt cggtcatgat
    56521 ggtcgccggg ttggagttca ccagaatgac gcggtaaccc tcttcgcgca gggctttaca
    56581 tgcttgcgcg ccagagtagt caaactcaca cgcctgaccg ataacaatcg ggcccgcacc
    56641 cagaatcagg atacttttta tatctgtacg ttttggcatg gctcttttac tcctgattac
    56701 ttagcggttt tacggtactg ctcaattaac ttgataaagt ggtcgaacaa cggcgcggcg
    56761 tcgtgtggac cagggctggc ttcagggtga ccctggaagc tgaacgccgg cttatcgatg
    56821 cgatgaatgc cctgtaacgt accgtcgaac agggatttat gcgtgacacg caggtttgca
    56881 ggtaatgttg cttcatccac cgcaaaaccg tggttctggg cggtgatcat caccacgttt
    56941 ttctccacat ctttaaccgg atggttgccg ccgtggtgac caaatttcat tttgacagtc
    57001 ttcgcaccgc tcgccagcgc cagcagctga tgaccgagac agatgccgaa taccggaata
    57061 tcggtttcga ggaacttctg gatggcggta atggcgtaat cgcacggggc cgggtcgcca
    57121 ggaccgttgg agaggaagat gccgtctgga ttcattttca gcacatcttc cgcagaagtt
    57181 tgcgccggaa cgatggtcag gcgacagcct ctatccacca gcatccgcag gatgttgcgc
    57241 ttggcaccaa aatcataagc cacgacgtgg aacggcagct cgtcttcttt tttcgcttct
    57301 ggcaggccgc cggtcaacgt ccagctccct tgtgtccagc tataggcttc tgcggtggtc
    57361 acttcttttg ccagatccat gccgttcaga cctgggaacg cgcgggcttt ttctaacgcc
    57421 agcgccgcat ccgggttatc gcctgcgata atgcagccat tctgtgcgcc tttctcgcgc
    57481 agtaaacgcg tcagcttacg ggtatcgata tcggcaatcg ccacgatgtt atggcgcttc
    57541 aggtaagaag agaggtcttc ggtattacgg aagttgctgg caatcagcgg caggtcgcga
    57601 atcaccagac cttgtgcatg tacctgagaa gattcttcat cggcgtcatt agtgccgaca
    57661 ttgccaatat ggggataagt aagagtaacg atttgacgag aataggaagg atcagtgagg
    57721 atttcttgat aaccggtcat tgaagtattg aaaacgactt ccccaaccgc cgaacctgtt
    57781 gcccctatgg cccgaccgtg aaactgggtt ccgtcttcca gaaccaatag cgctgactta
    57841 atcaaaacac cctccagaga atactcactc actttatttg catattaatt cacagtgatg
    57901 acgtaaatca atgcaaatct gcttaccgac gaatttctgg caaacggcgg cattctggag
    57961 atatgggcgc taaaagtcaa cttaatggca ggatttttta tattattttg tggctcatgc
    58021 atgttttaca ggcattaagc gacaaaaaca tcatctaacc tgttctggaa aacgcttgcg
    58081 cagcaagaag attgcatgaa ataaaaacga gcaggaaaaa gtggaccaaa tggtcaaaat
    58141 ttacattaaa acaacaaaaa tagaagacga aacatgtaat ttttggcagt tatagaaaat
    58201 ttaaaagcat aacaccaaaa ataaaagggc taaaaattaa agccctttat aattaataaa
    58261 ttaaatgtgt tacttttgca tcataataaa cattttgtgg ttacaaatta ttgagatcaa
    58321 gcacatctcg catatcaaaa agaccacttt ccttaccact caaccacaaa gccgatctta
    58381 ccgcaccgtt agcaaatgtc atgcggctgg acgccttatg ggtgatctcc agacgctcgc
    58441 caatatcggc aaacatcgcg gtatgttcgc caacgatgtc acctgcacgc acggtagcaa
    58501 aaccaatggt acctggcaca cgttcaccgg tatggccttc acgactgtag accgcgcaat
    58561 ctttcagatc tttatcaagg gcgtgggcga tcgcctctcc cattgccagt gcggtgcctg
    58621 acggcgcatc aactttatgt ctatgatgtg cttcaataat ttcgatatcg gtgtagtcac
    58681 ccatcacttt ggctgctttc tccagcagct taagcatgac gttaacgcca acgctaaaat
    58741 tggcggcaaa gacaatcgca atatcggcgg cggcgtcacg aattgcctgt ttaccggctt
    58801 cgtcaaaccc cgtagtgccg atcaccatcc ctttgccatg ctgccgacaa aaagcgagat
    58861 ggttcagcgt accttccgga cgggtaaaat cgataaacac atcaaaatca tcttttatcg
    58921 catcaagact gctttgcacg gtaacgcctg ttttccctgc accagccagc tcaccggcgt
    58981 cgctgcccag taaagaagat ccttcgcgct ccagcgccgc gcccagctgc acgccttcta
    59041 atgccagtgc cgcctgaatc aactggcggc ccatacgccc cccggctccc gcgatggcaa
    59101 cgcggatgtt tgcatcatgc atagctattc tcttttgtta atttgcatag accgttttca
    59161 gagtaaccag cgcatcgcta cgccgccagc caaaaacgct gataattaga aattaatgat
    59221 aaacagggag ggatatcagt gaaaggcatg agtcgatgaa tgactgcatg ccgataacat
    59281 gtgacaggtt acggcaccag agccagcacc tcagccaccc actgttggaa gccttttaca
    59341 ttcagatcca atgccacctg tacattggct ggtttgccca ggcaaccgtc gatatcaacc
    59401 accgtcgtgc ctgaggtaaa ttcgccttga gtttctaccg cgacaaaaca cggcttgagg
    59461 gtgaataagt ccgggcgcac cagccaggcg atggcgcaga gatcgtgcat tcgcagaccg
    59521 ctttgcatac tgccgctacg gtagtggcta aacagggcgt ggagcatttt tccggtgcga
    59581 ttcaactccg gtaaagtagc gagatagtca ggggttaata ttgcctgatt ggtgacatcc
    59641 agaccgcaca tgacaatttc gatgccgcta cggaagacac aggcagccgc ttctggatcg
    59701 gcagcgatat taaactcggc atttggtgta cagttgccgc gaccagcaga accgcccatg
    59761 atcaccagac gacgaatata cggtttacat tccgggcatt gtaaaagtaa cagcgcaata
    59821 ttggttaacg ggccgatggc caccagggta acgggctctg gtgcacgcat cagggcatcc
    59881 cgaatcgcca gaaacgccgg tatcccgagt ggctttcggt tgtgctcaac aaagtcgtag
    59941 ccagccattc ccgattcgcc gtgcacagat gctgcatcac gcggtgcgcg taccagcggc
    60001 acagcggctc cctgggcgag cggaatctcc gcattccaga aatgcagcag ttgcagggca
    60061 ttgcgggtag ttttctcaac cgagacatta cccgcgacgg tggtcatcag ttgcaggtcg
    60121 agttcggggg caaaaatcgc ggcggcaatg gcgacggcat cgtcaatgcc ggggtcagta
    60181 tcgaggaaga taggtaaacg catgttttct tcataaaaaa tgccggtaac aagaccggca
    60241 ttttcgcata acttaggctg ctaatgactt aatcgacttc acgaatatcg acacgcagct
    60301 ctttcggcac ttcgaaaaca atattttctt cacggccttc cagcggaatg gcttcaccac
    60361 cacccaactg ctgcaaacgt gccaccacat tctgcaccag aatatccgga gccgatgcgc
    60421 ccgcagtcac gccgacgcat ttaacctctt tcacccactc ttcctggata tctttcgcat
    60481 cgtcaatcaa aaacgcgtgt ttgcccatac gctgggccag ctccgccaga cggttggagt
    60541 tggaggagtt tttcgaaccg accaccaaca caacttccgc ctgttctgcc agggcgcgta
    60601 ccgcttcctg acggttagtc gtggcgtagc agatgtcatc tttgcgcgga ccgacaattt
    60661 tcgggaagcg tttacgcagc gcgtcgatca catcagacgt gtcatccacc gacagcgtgg
    60721 tttgggtcat aaaggagagc ttctcttcgt ttttgaccgt cagtttccac acatcgtctg
    60781 gcgattcgac tagatacatt cccccttcgg ggttgctgta ctgccccatc gtcccttcca
    60841 cttccgggtg cccggcgtgg ccgatgagaa tagattcttc gccacggcga ctggcgcggg
    60901 caacttccat atgcactttg gtcaccagcg gacaggtggc atcaaacacc gtcaaatcgc
    60961 ggcttttcgc ttcgttacga accgcctgag aaacaccgtg tgcggagaaa atcaggatcg
    61021 cgccgtccgg cacttcgcta atctgctcaa taaagatagc cccacgctcg cgcaagctat
    61081 cgaccacgta gcggttatgc accacttcgt gacggacata tatcggtgcg ccgtaaatgg
    61141 ccagcgcgtt ttcaacaatg ctgatagcgc ggtctacccc ggcacaaaaa ccacgcgggt
    61201 tggccaacag gatctgcatg ttacgcctcc agtgccggat cgatttccag cacttcaata
    61261 tcaaaatgaa cggtctgccc ggccagcgga tggttgaaat caacggtaat ggagtcgccg
    61321 ttaatttcgc ggatcacacc aggcatctca ctgccatcca ttgcggtaaa aagcatgatt
    61381 gcgccaattt ctggctcgcc tgcatccata aattcacggc gggagaagta ctgaatcagg
    61441 tccggtgacg gcacgccaaa cgccgcatct ggctccagcg agaaggtggt tttgtcgccc
    61501 actttcagcc ccagcaggtg ttgctccagc ccttcagaaa gagaagcatc gcccaggcgg
    61561 aacagcgccg gtttaccgtt gttgcgagta gactcggcgg tggtgccatc gtcgagtttt
    61621 agcgtgaagt gcaccaggac ggcgctattg ctctgtacag attcagacat gcaggtttgc
    61681 tcttattttt gaggattttt gccggatgcg gcctacgatt tcgtagcaat ctgtaggccg
    61741 catccggcag gatttattat tgttttttcg ctttggaagg taaaaaccct tccagcacga
    61801 tcagtgccgc accgacacaa atggcagtat cggcaaggtt gaaggtggcg aagtgccagt
    61861 cgccgacgta gaagtcgatc atatcgacaa cgaagccgtg ccacaggcgg tcgaacaggt
    61921 tgcccagcgc gccgccaata atcagcgcgt aagcgatatt gtttagcttc tgcgtggcct
    61981 tcgagcgata catcatcact gccaggagca cgctaatacc aatcgcaata ccggcaaaga
    62041 accaacgctg ccagccgccg ctatcggcaa ggaaactaaa cgccgcgcca tagttacgcg
    62101 cataatgcag attaagcgac gggaacagcg ggaccgtatc ccccagagca aagttctgga
    62161 ggatcaggta tttgctgccc agatcgataa tcagcacgac taccaccagc cacagccagc
    62221 gtagccctgt tgaacagatc gattgactca tcaggcaaac ttacgttttt caccgtcacc
    62281 ggcgacgttg ctgacacagc ggccgcagat ttctgcgtgt tccgccacct tgccgacatc
    62341 ctgggtgtag tgccagcagc gtgggcactt ctcaccttcg gctttactca acgcgacttt
    62401 cagccctttg agtacttcgc tctgctgagc atcagcaggt gcgtcgttat agtctgcaac
    62461 ggtagcgccg gaggtcaaca ggacaaatcg taattcatcg cccagcgcgg tcagtttcgc
    62521 cgccagttcc ggttctgcat acaaggttac tgccgcttcc agcgagccac ccactttctt
    62581 gtcggcacgc gcttgctcaa tgactttgtt cacttcgcca cgcactttca acagctcgtc
    62641 ccagaacgca tcgttcattg cttcactgtc tgccagacca aacaggcctt cgtaccactc
    62701 accggtgaag acgtattttt cacgttcgcc cggcagatag ccccacactt catcagcggt
    62761 gaaggagagg attggtgcca tccagcgcac cagcgcttca gcgatgtgat acagcgcagt
    62821 ctggcagcta cgacgcgcca cgctatccgc tttggcggtg tactgacggt ctttgatgat
    62881 gtcgaggtag aaggaaccca tctcaacgga gcagaagcgc atcagacgct gtaccacttc
    62941 gtggaaatcg tatgcttcgt acgccttgag gatgtcttcc tgtgccgctt tcgcacaacc
    63001 tacggcccag cgatccagta ccaccatctc ttccggtttc accatatctt ttgctggatc
    63061 aaaaccattc aggtttgcca gcaggaagcg cgcggtgtta cggatacgac gatagctatc
    63121 ggcagcacgt ttcaggatct cgtcagaaac ggccatttca ccggtgtagt cggttgatgc
    63181 cacccacaga cgcagaatat ccgcgcccag tttgttcatc acatcctgcg gcgaaacggt
    63241 gttgccgatg gatttagaca tcttgcgacc ctgaccatcc acggtaaagc cgtgggtcag
    63301 tacctgacga tacggcgctt tgcctttcat cgcggtggag atcattaggg aagacatgaa
    63361 ccaaccacgg tgctggtcag aaccttccag atacatgtcc gctgcgtgac cggcaaattc
    63421 cggacgcacg tcaacaacag aagagtgggt agatccggag tcaaaccata cgtcgagagt
    63481 atccggcact ttcacatact ggtcagcttc gtcgccgagg atctctttcg catcgagatc
    63541 ccaccacgcc tggatgccgt caacttcaac gcgtttagcc acttcttcca tcagttcgag
    63601 ggtacgcgga tgcagctctt ccgtgtcttt gtgcacgaac agtgacatcg gtacgcccca
    63661 ggtgcgctga cgggagatac accagtcagg acggttagca accatcgact cgatacgcgc
    63721 ctggccccag tccgggagcc actgcacgcc tttgatctct ttcagtgact gcgcacgcag
    63781 acctttctga tccatgctga cgaaccactg cggtgtcgcg cggaagatga tcggcgtttt
    63841 gtgacgccag cagcacggat agctgtgctg cattttctca acgtgcagca gcgcgccttt
    63901 ttcctgcagc agcgcaacga cgatgtcgtt cgctttgaag acgttcacgc catccagcgt
    63961 cggataagtg cccggcagat aagtgccgtc cgggccaacc gggttagcgg tttccaggcc
    64021 gtatttctga ccgatcacat agtcgtccgg gccgtggcca ggcgcggtgt gaacggcacc
    64081 ggtaccggca tccagggtaa cgtgatcgcc gaggattgcc ggaacgtcga agcccataaa
    64141 cggatgggta aagcgcagca gctcaagctc tgcacctttt accgtgccga gaatggtgta
    64201 atcggtcacg ccgatacgct gcattacgct ttcaaccaga tctttcgcca gaatcacggc
    64261 ctgaccgtcg atctgcacca gcgcatagtc gaaatcaggt gcaatagaga ttgcgcggtt
    64321 cgcaggcaga gtccacggcg tagtggtcca gatcaccagc gagattgggc cgttaacgtt
    64381 gcttacgcca aatttcgctt tcagcgcatc ctgatcgacc gcctggaaag cgacgtcaat
    64441 ggacggagaa gttttgtcgt aatactcaac ttccgcttct gccagtgcag aacggcagtc
    64501 tacgcaccag tgcaccggct tcgcgccttt gtgcaggtga ccattgccga tgattttgcc
    64561 cagcgcgcgg atgatgttgg cttcagtttt gaagtccata gtcagatacg ggtgcgacca
    64621 gtcgcccagc acgcccagac ggataaagtc tttgcgttga ccgtcaacct gggtcgccgc
    64681 gtattcgcgg cacttggcgc ggaactcggc ggcggtgaat ttctcacccg gcttaccgta
    64741 ttcttgctct actttcagct cgatcggcag accgtggcag tcccagccag gcacatacgg
    64801 cgagtcataa ccggaaagcc ctttggactt cacgataatg tctttcagaa tcttgttaac
    64861 cgagtgacca atatgaatgc tgccattcgc ataaggaggg ccatcatgca gaatgaaggt
    64921 ttttttgcct tttttagccg cacggatgat gccgtacaga tcatcatcag tccaacgcgc
    64981 cagcattccc ggttcgcgct tggcgagatc gccacgcatc gggaaccctg tttccggcaa
    65041 attcagggtt gatttatagt cactcatcag attctcggtt ccgtgtttcg gtttgattac
    65101 ataacaggct taagccggtt ttgttagccc aaaaaattcg cgggcggtta attcatcacg
    65161 cgcaatctgc gctttcagtt cgtccagcga cgcaaatcgc tgctcattgc gtattttttt
    65221 acgcagcact acttgtatat ggcgaccgta aaggtccatt gcaacatcta acaaatgcac
    65281 ttccagctgc tggcgaatac cggcaaccgt tgggcgtgtt ccgatgtttg ccacgccggg
    65341 taacggcttt tcaccgaggc ccagcacttc taccgcataa acccctttca ccggggaaac
    65401 ctgacggcgt agcggtacat tcgccgtcgg gaaacctata gtgcgcccta attcatcacc
    65461 gtggactaca cgcccggaga tggcaaacgg gtgccccagt aaactttctg ccagagccag
    65521 attgtcatca gccagcgcct gacgcacggc ggtgctgctg atacgcacac caccttcgca
    65581 aaaagtttgc gtactggtga tatcgaagcc gtattccatg ccagctttct gtaataacaa
    65641 gaaatcgcct tcacgaccag cgccaaagcg gaaatcatca cctacggcaa gaaattttac
    65701 gcgcaaatgc ttcaccaaaa gatcgctgat gaaattttgc gcggttaacg ccgcaaaacg
    65761 cctgtcgaaa cgcacgcaca gcacgtaatc aacgccacac tctgcaaggt aacgcagttt
    65821 ttcccgcagc cgggtcagtc ttgccggggc tttatcggta gcaaacagtt ccagtggttg
    65881 aggttcaaaa agcatcacca tcaccggtaa gttgcgcttg cgcccttctt cctgcaagcc
    65941 ctgtaacagc gcgcgatgac cgcgatgcac gccgtcgaaa ttaccaatag tcagcacaca
    66001 cccttcttgc ggggcctggc tgagattatg tatgccgcgt atcagcttca tgtctggctc
    66061 aaaacagtga aaatcgtccg agtatacctt gtacagcggt caaggttaac cggcgattga
    66121 gtaccgaaat cagaaagaag tggcttttca ttgccatgga gcaaatcacg ggaagaaact
    66181 gaccgcctgc tgcaattttt atcgcggaaa agctgtattc acaccccgca agctggtaga
    66241 atcctgcgcc atcactacgt aacgagtgcc ggcacattaa cggcgcttat ttgcacaaat
    66301 ccattgacaa aagaaggcta aaagggcata ttcctcggcc tttgaattgt ccatatagaa
    66361 cacatttggg agttggacct tggctaatat caaatcagct aagaagcgcg ccattcagtc
    66421 tgaaaaggct cgtaagcaca acgcaagccg tcgctctatg atgcgtactt tcatcaagaa
    66481 agtatacgca gctatcgaag ctggcgacaa agctgctgca cagaaagcat ttaacgaaat
    66541 gcaaccgatc gtggaccgtc aggctgctaa aggtctgatc cacaaaaaca aagctgcacg
    66601 tcataaggct aacctgactg cacagatcaa caaactggct taatcgccaa tttgctgaag
    66661 ctttgtgaaa aagcccgcgc aagcgggttt ttttatgcct gctgcttttg tgatgcgtga
    66721 gagaacacac tgccggttgc cagatagtca aaagagacat gccgttgtac tatctgaact
    66781 cttcgtggct taagaatcgt agaaccatcg gctgatacat tctgaacaag aatatctact
    66841 caatctccag actcccccac aaagaatatg gatattgtga tacacattga ggtattaaac
    66901 ttaagaccac tctatttttg aattaaattc ctatatactt taaccattca taaatatctc
    66961 ctcattagac c
//
LOCUS       DAJTSA010000009        66642 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000009 DAJTSA010000000
VERSION     DAJTSA010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.009"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO369_000622"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO369_000622"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR5981008.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO369_000623"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO369_000623"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR5981009.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO369_000624"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO369_000624"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR5981010.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO369_000625"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO369_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR5981011.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO369_000626"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO369_000626"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR5981012.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO369_000627"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO369_000627"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5981013.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="OO369_000628"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="OO369_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR5981014.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO369_000629"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO369_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5981015.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO369_000630"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO369_000630"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR5981016.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO369_000631"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO369_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR5981017.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO369_000632"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO369_000632"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR5981018.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="OO369_000633"
     CDS             complement(15665..16702)
                     /locus_tag="OO369_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5981019.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="OO369_000634"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="OO369_000634"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5981020.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="OO369_000635"
     CDS             complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="OO369_000635"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR5981021.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19444..19884
                     /locus_tag="OO369_000636"
     CDS             19444..19884
                     /locus_tag="OO369_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR5981022.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="OO369_000637"
     CDS             complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="OO369_000637"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5981023.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20611..21601)
                     /locus_tag="OO369_000638"
                     /pseudo
     CDS             complement(20611..21601)
                     /locus_tag="OO369_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="OO369_000639"
     CDS             complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="OO369_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR5981024.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="OO369_000640"
     CDS             complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="OO369_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR5981025.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="OO369_000641"
     CDS             complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="OO369_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR5981026.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25143..25856)
                     /gene="livF"
                     /locus_tag="OO369_000642"
     CDS             complement(25143..25856)
                     /gene="livF"
                     /locus_tag="OO369_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR5981027.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25858..26625)
                     /gene="livG"
                     /locus_tag="OO369_000643"
     CDS             complement(25858..26625)
                     /gene="livG"
                     /locus_tag="OO369_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR5981028.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26622..27899)
                     /gene="livM"
                     /locus_tag="OO369_000644"
     CDS             complement(26622..27899)
                     /gene="livM"
                     /locus_tag="OO369_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR5981029.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27896..28822)
                     /gene="livH"
                     /locus_tag="OO369_000645"
     CDS             complement(27896..28822)
                     /gene="livH"
                     /locus_tag="OO369_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR5981030.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28870..29979)
                     /gene="livK"
                     /locus_tag="OO369_000646"
     CDS             complement(28870..29979)
                     /gene="livK"
                     /locus_tag="OO369_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR5981031.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30403..30786
                     /gene="panM"
                     /locus_tag="OO369_000647"
     CDS             30403..30786
                     /gene="panM"
                     /locus_tag="OO369_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR5981032.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="OO369_000648"
     CDS             complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="OO369_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR5981033.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="OO369_000649"
     CDS             complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="OO369_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR5981034.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="OO369_000650"
     CDS             complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="OO369_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR5981035.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="OO369_000651"
     CDS             complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="OO369_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR5981036.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="OO369_000652"
     CDS             complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="OO369_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR5981037.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36826..37422
                     /gene="rsmD"
                     /locus_tag="OO369_000653"
     CDS             36826..37422
                     /gene="rsmD"
                     /locus_tag="OO369_000653"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR5981038.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37412..37681
                     /locus_tag="OO369_000654"
     CDS             37412..37681
                     /locus_tag="OO369_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR5981039.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37684..38043)
                     /locus_tag="OO369_000655"
     CDS             complement(37684..38043)
                     /locus_tag="OO369_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR5981040.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38184..38810
                     /locus_tag="OO369_000656"
     CDS             38184..38810
                     /locus_tag="OO369_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR5981041.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38884..41082
                     /gene="zntA"
                     /locus_tag="OO369_000657"
     CDS             38884..41082
                     /gene="zntA"
                     /locus_tag="OO369_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR5981042.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="OO369_000658"
     CDS             complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="OO369_000658"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR5981043.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41650..42315
                     /locus_tag="OO369_000659"
     CDS             41650..42315
                     /locus_tag="OO369_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR5981044.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42388..42945
                     /gene="dcrB"
                     /locus_tag="OO369_000660"
     CDS             42388..42945
                     /gene="dcrB"
                     /locus_tag="OO369_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR5981045.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42949..44166)
                     /locus_tag="OO369_000661"
     CDS             complement(42949..44166)
                     /locus_tag="OO369_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5981046.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44300..45349
                     /locus_tag="OO369_000662"
     CDS             44300..45349
                     /locus_tag="OO369_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5981047.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45404..45991
                     /gene="acpT"
                     /locus_tag="OO369_000663"
     CDS             45404..45991
                     /gene="acpT"
                     /locus_tag="OO369_000663"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR5981048.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46102..47676
                     /gene="nikA"
                     /locus_tag="OO369_000664"
     CDS             46102..47676
                     /gene="nikA"
                     /locus_tag="OO369_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR5981049.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47676..48620
                     /gene="nikB"
                     /locus_tag="OO369_000665"
     CDS             47676..48620
                     /gene="nikB"
                     /locus_tag="OO369_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR5981050.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48617..49450
                     /gene="nikC"
                     /locus_tag="OO369_000666"
     CDS             48617..49450
                     /gene="nikC"
                     /locus_tag="OO369_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR5981051.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49450..50214
                     /gene="nikD"
                     /locus_tag="OO369_000667"
     CDS             49450..50214
                     /gene="nikD"
                     /locus_tag="OO369_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR5981052.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50211..51017
                     /gene="nikE"
                     /locus_tag="OO369_000668"
     CDS             50211..51017
                     /gene="nikE"
                     /locus_tag="OO369_000668"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR5981053.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51023..51424
                     /gene="nikR"
                     /locus_tag="OO369_000669"
     CDS             51023..51424
                     /gene="nikR"
                     /locus_tag="OO369_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR5981054.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51544..51903)
                     /locus_tag="OO369_000670"
     CDS             complement(51544..51903)
                     /locus_tag="OO369_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR5981055.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51900..52175)
                     /locus_tag="OO369_000671"
     CDS             complement(51900..52175)
                     /locus_tag="OO369_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR5981056.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52234..53358)
                     /locus_tag="OO369_000672"
     CDS             complement(52234..53358)
                     /locus_tag="OO369_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5981057.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="OO369_000673"
     CDS             complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="OO369_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR5981058.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56090..57157)
                     /locus_tag="OO369_000674"
     CDS             complement(56090..57157)
                     /locus_tag="OO369_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5981059.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57523..59145)
                     /locus_tag="OO369_000675"
     CDS             complement(57523..59145)
                     /locus_tag="OO369_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5981060.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59407..60996)
                     /locus_tag="OO369_000676"
     CDS             complement(59407..60996)
                     /locus_tag="OO369_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5981061.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61381..62412
                     /locus_tag="OO369_000677"
                     /pseudo
     CDS             61381..62412
                     /locus_tag="OO369_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62499..62975)
                     /locus_tag="OO369_000678"
     CDS             complement(62499..62975)
                     /locus_tag="OO369_000678"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981062.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62968..63732)
                     /locus_tag="OO369_000679"
     CDS             complement(62968..63732)
                     /locus_tag="OO369_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR5981063.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63978..64739
                     /locus_tag="OO369_000680"
     CDS             63978..64739
                     /locus_tag="OO369_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5981064.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(64846..65346)
                     /locus_tag="OO369_000681"
     CDS             complement(64846..65346)
                     /locus_tag="OO369_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981065.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(65497..>66642)
                     /locus_tag="OO369_000682"
     CDS             complement(65497..>66642)
                     /locus_tag="OO369_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5981066.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT        16187 a        17888 c        16351 g        16216 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcaca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tacctggctg attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctctaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagctgcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcagc cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctacttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattgc cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat tgcgatacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cacccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgtcgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc aggaggcgcg gcggcggcgc
    19261 taaaacaact tcctgagagc agagcagcaa tggccacccg gcgtaaaaac gtcggtttta
    19321 tcatcgttat tctccagaga ttaaggggca accccagcta agcctggtat ataactctga
    19381 attaatcatc gttttgtcgg gaagcgagta aacttaaagg atatcttcag aggaggatac
    19441 gcgatgaaac gacttctgat tcttacggca ctcctgccgt ttgtcggctt tgcacagccc
    19501 attaatactc tgaacaaccc taaccagccg gggtatcaga tacccagcca gcagcggatg
    19561 caaacccaga tgcagactca gcaaatccag caaaaaggga tgctgaatca gcaactgaaa
    19621 acgcaaactc agttgcaaca gcagcattta gaaaaccaga taaacaataa ttctcagcgg
    19681 gtgttgcagt cacagccggg ggagcgaaat cccgcccggc agcaaatgct gcccaacacc
    19741 aacggcggga tgttaaacag caaccgtaat ccggatagtt cgttgaatca gcagcatatg
    19801 ctgccggaga ggagaaacgg cgacatgctg aatcagccca gcacgccgca gcctgacatt
    19861 ccgttgaaaa ctattgggcc gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga
    19921 tgcaatccac gccccagcgc agcaactctg ccgcgtgctg gggtttattg acggtataaa
    19981 ccagaatccg taatccggcg tctttcaact gcatcactcg cgctttatcg agtaacttat
    20041 gattgagatg aatagagacg cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc
    20101 actcatcaag caataaacca cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag
    20161 catcaatctc aaacgatgac agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg
    20221 ccagcgccac cattttgccc gttaatggtc cagtgccagt ggtgggtttg atttcgatat
    20281 tcgccatcat cccgtgttcg cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct
    20341 cacctttaaa cgctttgctg taccaactgc ccgcatccac gcgcagtaaa tcctgccagt
    20401 tcagttcacc cgcgacgccc cagccgttgc tggtgcgttc gagattgtcg tcatggagca
    20461 ggaagatctc gccatctttc gataacttcg cgtcaaattc gatcatctta tgaccgtatt
    20521 ttgccccgac gtcgattgcc gccagggtgt tttccggggc cagcttaccg ccgccacgat
    20581 gagcgacgat gcggggataa ggccagttac tcatactcgt tgtcctgttt caccatcaaa
    20641 aagatgcagc tgattttccg ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc
    20701 ctgatgcgcc agtcgcacca ccagcttctg cttgcgagct tagcgcaata tgttccgggc
    20761 gaatgccgag ggtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca
    20821 cgataccgcc gtccagttcg aaatgcgtgc cttcattatt catgcggcct gtcagcaggt
    20881 tcatcgccgg gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt
    20941 ccaccggcgt gccaatctgt tcggcaacgc cgccgttcat caccattact cgctgggcga
    21001 gcgtcatcgc ctcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt
    21061 gcagctgttg cagttcaaga cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg
    21121 gctcatcaaa caagaacacc gccggatcgc gcacaatcgc gcgtcccatc gccacacgct
    21181 ggcgctgacc gccggaaagc tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa
    21241 tgcgcgccgc ttctttaacg cgctcggcta tttgctgctt gcccatgccg cgaattttca
    21301 gcccccacgc catgttttct tcgacgctca tatgcggata aagcgcgtaa ttctggaaca
    21361 ccatcgcaat cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga
    21421 tatcgccttc ggtcaccctc tccagcccgg caaccatacg cagcagcgtc gatttcccgc
    21481 agccagacgg cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt
    21541 taatgaccta ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca
    21601 tcttatttct cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc
    21661 actactggtg ggatcagggt taacaacatc gccgccatca ctgagttcca ttccgtggtg
    21721 ccttcgccag tggcgatcat ccctttgatc cctgccacgg tggtgccgag atccacgtcg
    21781 gtaataatca acaacggcca caaatactgg ttccagccgt aaataaaggt gatcacaaac
    21841 agcgccgcca gattagtttt ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt
    21901 gacgcaccgt cgatccgcgc cgcttccacc agttcatccg gcagcgtcat aaagaactgg
    21961 cgaaacagaa acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg
    22021 tcgagcatct tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc
    22081 agcatcaggg tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag
    22141 acaatggcaa atgccgagag catcgagaca gtaattttgc cgagcgtaat gctgaacgcc
    22201 atcacaaagc tgttaagcaa catccgccag aacggcgcgc tatttgtgcc taccccgttc
    22261 acccagatgt tgtggatgtt ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg
    22321 gcgtagacgg cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg
    22381 atcaccgcga tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc
    22441 tcaatcattg gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga
    22501 tgacgaggaa catcagcacc atcgactgtg cggcagacga agccagatcc agtccggtaa
    22561 aaccttcgcg gtagatctta taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg
    22621 tggcggcgtc gatcaccggg aaggtgtcga agaaggcata caccaggttc actaccagca
    22681 ggaagaaact caccggggcg ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc
    22741 ctgcaccgtc gatggctgcg gcttcgatca acgaacgggg aatggattgc agcgcggcat
    22801 agaagaacag aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca
    22861 tcgcctgacc gctattttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg
    22921 tgatcagccc gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag
    22981 ccacggcata cggcagcaac attaaggttt ggtagaaacg actgccgcgc acgatgtact
    23041 ccaccagcgc cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga
    23101 attttatcgt cgtccagaag gagtcgagat aatagctgtc atgaaacagc gtgacgaagt
    23161 tatccagacc gacaaactgg ctggagaaac caaacggatc gacgctttgt agcgagtacc
    23221 acaacgcttc gcccgcaggc cagataaaaa agataacggt gatgatgagc tgcggcgcga
    23281 ccagcagata aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta
    23341 attcctgaac tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga
    23401 taagcgaaac gcatccggca ttacactgat taagacttcg tcgatttctc aaagcggcgc
    23461 agtaactggt tcccacgctc aactgcggta tccagcgcct gctgtggcgt cttcttaccg
    23521 gtccacacgc tctcaagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga
    23581 cgaagccctt tggtgaacgg caacggcggc ttgttcagca tctgacgcgt cgcggtatcc
    23641 gcacctgggt ttttctcgta aaagccctgc tcacgggtca ggtcatacgc cgctttggta
    23701 attggcagat aaccggtttt ctgatgccac tcagcagcgt tttctggctt cgcgaggaag
    23761 tcgagaaact tcgccacgcc ggtatacgtt tctttatctt tgccctgcat cacccacagg
    23821 ctggctccgc cgataatggc gttttgtggc gcatctttcg catcggcgtc gtaaggcatc
    23881 atacctacgc cgtagttaaa tttggcgtac tcgcgaatgt tggcaagaga accggaagag
    23941 gcggtggtca tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg
    24001 acgtagctga agtcgccctt cttgttcatc tcctcgagca tggcgatgtg tttcacctgc
    24061 tccggcttgt tgaactccag caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca
    24121 aacggcagac cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg
    24181 gcgtagccgc acttaatacc ggaggctttc agttttgcgg aatagtccgc cagatcctgc
    24241 caggttttcg gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa
    24301 tagagaacgg aagtcgagct gttgaacggc tgggagagta agtggccggt tttgctgtcg
    24361 gagtagtaac cggaaaccgt cggcacaaac tgcgactcat cgaactgaat ccctgcctct
    24421 ttaaacacgt catacaccgg tttaatggct ttcgacgcca tcatggtggc ggtgccaact
    24481 tcataaacct gcaaaatagc cggagcgttg ccggtacgaa atacggcaat ccccgcgctt
    24541 aaattctgtt cgtagttgcc tttataggtc ggtacaattt tgtaatccgg attttcggcg
    24601 ttaaaacgtt gggccagaga atccacctct ttacccagtt ccccttccat agaatgccag
    24661 aacggaatgg tcgtcactgc ctgtgcattc cccattaacg ccagtccgag cgccagtgct
    24721 gaagctgtat aatgtaacgg tttcatcgtt tatctctctt gttgtaccga atgcgcgaat
    24781 tcacgcgttt tatgctcgcg gggtaacatg acatgctcga attacagaaa aataactttt
    24841 ttgttacatt tgtaagatag taaggtgtca gaaagatgac aaggcggtga cggcgtgggt
    24901 gagggaaaat gggagatggg gcacggataa gcggaaaaat agaaggtctg aatcaaactc
    24961 tacagattac tcatcgtttc atgccggatg cggcgtaaac gccttatccg gcctacaaga
    25021 tcgtgcaaat tcaatatatt gcaactcacc cagtaggcct gataagcgca gcgcatcagg
    25081 caattttgca tttgtcacct gtctcaaagg agtcttttga ctccttatca atcaacgtgt
    25141 tattacccgc ctaaatacgc acttctcact gcttcgttcg ccagcagcgc gtcgccggta
    25201 tcggagagca ccacatggcc gttttccagc acgtagccgc gatccgccag ctttagcgcc
    25261 tggttggcgt tctgctcgac gaggaagata gtcatcccct gctcgcgcag ctgctcgatg
    25321 gtgtcgaaaa tttgctggat gataatcggc gcaagaccga gcgatggctc atcaagtaac
    25381 aacaaacgcg gattgctcat cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg
    25441 gacatggtgc ccgcccgctg aacacggcgc tcatgcagac gtggaaacag ttcatacacc
    25501 cactttatgc gctcctgaaa ctggtcacgt tcggcaaaga agccgcccat cgccaggttc
    25561 tcttccaccg tcatccgcga gaagacgcga cgcccttccg ggacaatcgc taccgcttcg
    25621 cgcatgattt tcgccgtctg ccagtcggta atgtctttat catcaaacac aattcgcccg
    25681 ctggtggcgc gcggatcgcc gcataacgtg ccgagcaagg tggttttccc cgccccgttc
    25741 gcgccaatga gagtgacaat ctcgccctga ttgatatgca ggctcacctc atgcagcgcc
    25801 tggattttgc cgtagtgggc gctgactttg tcaaaggaca acatgacttt ttccatctta
    25861 tgcctcacct aaataggcac ggatcacgtc cgggttatta cggatttgct ccggcgtacc
    25921 gtttgccagt ggcgtcccct gattgaccac ataaattcgg tctgaaatcc ccatcaccag
    25981 cttcatatcg tgttcaatca acaaaatagt ggtgttgtga tgattgcgca gttcggcaat
    26041 cagctcatcc agctctttgg tttcttttgg gttaagacct gccgccggtt cgtcgagcat
    26101 taaaatctcc ggctgcgtca ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata
    26161 ggccaggtta ctcgcctgac ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt
    26221 ggcggcgcgg tcgagcgctt cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc
    26281 agagaacagc ccggttttca gttgctgatg ctgcgccacc agcaggtttt caattaccgt
    26341 catttcacgg aacagacgca catgctggaa ggtgcgcacc acgcccatgc gagcaatttg
    26401 ctgccccggt aaaccttcaa gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt
    26461 gtagaaaccg gtcaggcagt taaaaaccgt ggtttttccg gcaccgttag ggccgattaa
    26521 cgagacgatt tcctgcgggt acagttcaag attgacgttg ttcaccgcca gcaggccgcc
    26581 gaagcgcatc atcagaccgt taacagataa taatggctga ctcatgcctg ctctcctttc
    26641 gctgcgccgt ttttcagctt cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc
    26701 cagatcatca tcagcaccat caaaccaccg agcattaaca tgctgtattc gttgaaatca
    26761 cgcatcaatt cgcgcgacac caccagcaga acagccgcca gaatcactgc aaattgcgag
    26821 cccataccgc cgagcaccac tatcgccagc acaaaggccg attcagcaaa ggtgaaggat
    26881 tccgggctga caaagccctg acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg
    26941 gcgctgatgg taaaagcagt cagcttgata cggcgcgggc ttaagcccag cgaacgacag
    27001 gcgatctcgt cttcacgcaa cgcttcccac gcgcggccca gcggcatccg cagcaggcgg
    27061 ttaatgacaa acaggcttaa caccaccagc agcaacgcca ccaggtagag gaagataacg
    27121 cgatcggacg gatcgtattt caggccaaag aaattactga acgtgtccca gccgccttca
    27181 cgagcggtac ggctgaactc gagtccgaag agtgtcggtt tcgggatctg actgattccg
    27241 ttcgggccgc cggtgatttc agtgttattg agcagcaata tgcgcacaat ttcgccgaaa
    27301 ccaagggtaa cgatcgccag atagtcaccg cgcagacgca gcaccggaaa accgagcagg
    27361 aaacccgccg ccgctgccat cagtccggca atcggcaaac aggtccagaa ccccagaccg
    27421 taatagtgat tgagcagcgc aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc
    27481 agcaccagca gaccagaaag accaacaacc acattcagcc cgagaccgag gataatgtag
    27541 atcatggtca gagtggcgat atccaccgtc ccacgtgaca ccataaacgg ccacgccacc
    27601 acaagcacca gcagcgccac gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg
    27661 ggcagaataa acttcggccc ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa
    27721 agctggaaga aaaagaccac cgccgtgccg ataaacatcc actgccaacg gatgtctgaa
    27781 gccgtgtcga ccaccagttt ggtgccatcc agctccagtt gtacgcccat aaagacgccc
    27841 gccaggacaa agaacatcgc ggcagagagc agtgccattg caatatgcat cggtttcata
    27901 ctttctctac ctccgggcga cccagaatac cggtcggcat caccagcagc accagaatca
    27961 gcagggcgaa tgagaccaca tctttatatt ccgtactcag ataggcagaa gagagcgcct
    28021 ccgcaatccc caggatcagc ccgccaatca tcgcccctgg aatgctgcca atcccaccga
    28081 gcaccgcagc ggtaaaggct ttcatcccgg ccataaagcc gatatagggg ttaatgacgc
    28141 agtagaactg accgagcagt acgccagcca ctgccgccat cgccgcgcca ataacaaagg
    28201 tcaaagcaat cacccggtcg gtgttgatgc caagcaggct cgccattttc agatcttccg
    28261 cgcaggcacg acaggcgcgc cccatacggg aatagcgaat gaaaatcgtc agcgccagca
    28321 tggcgaggaa ggtgacaatc cagatcaccg cctgcatggt ggtaatagag gcagagaagt
    28381 tttcgctatg gcccaccacc cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg
    28441 aaccttcggt caggctgacg tagttttgca ggaagatgga cataccgatt gcggaaataa
    28501 gcgcaatcag gcgcttagag ttacgcaccg ggcggtaagc tacccgttcg atactccagc
    28561 cgtaggcgct ggcgatgacg attgcgccga cgaatcccgc cgccaccagc agccagccgg
    28621 tatcaatgcc catcatcatc agcgcggcga tgatcataaa tgagacgtag ctgccaatca
    28681 tataaacctc gccgtgggcg aagttgatca tgccgataat gccgtaaacc atggtgtagc
    28741 cgatggctat cagcgcgtag gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga
    28801 aatacaaaaa ctgctcagac ataaggtaac ctttctaaac ccgcccgcat tttacgggcg
    28861 gtgggatgat cacttggctg ccgtggatga accgtcggcg tgccactgga agacaccaaa
    28921 atcaaatccc ttaagatcgc ctttttcatc ccagttcagc ggcccaatca cggtgtttgc
    28981 accgttagct tttaaatctt tcaccagcgc cagcggctca tcgctgccgg tacgctcaag
    29041 ggcagttgcc agagattgca ctgccgcgta agtgatccag acataaggcc cggacggatc
    29101 tttcttgtct gctttcagcg catcaacgat gccctggttt gccggatcct ggtcatagcg
    29161 ttttggcata gtgaccaaca tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc
    29221 gttaccgaca ccttccggcc ccataaactg ggttttcagg ccaacggaac gggcttggcg
    29281 cagcatctgc cccatttccg ggtagtaacc gccgtagtaa acgaagtcga tattttcttt
    29341 tttcaggcgg gcgatcagcg cggagaaatc tttctccccg gcggtaatac cgtcgaagaa
    29401 gacgacgttg gcgttagccg ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc
    29461 atactgttgt ttgtcgtgaa tgatggcgat gcgctggggc ttcaccgtct caagaatgta
    29521 ttttgccgcc gtaggcccct gggaagagtc cagcccggca gtacgcataa tgtgttgata
    29581 accgcgttgg gtcagctccg ggttggtcgc tcccggcgag atcatcagaa taccttcgtc
    29641 ttcgtagata tccgacgctg gctgggtaga ggaagaacac agatgaccaa taacgtattt
    29701 aatgccgtcg ttaacgattt tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc
    29761 gtcatattcc acgccaacca gcttgtcgcc cttaattccc cctttgacat taatgtcttt
    29821 aatcgcctga cgcgcgccgt taaattccat atcgccccac tgggcaatcg ggccggacat
    29881 cgcgccgaca acggcgactt taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc
    29941 aatcatccct gcgatgatag ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg
    30001 atgttgtgtt gctttgtttt tatgtgttaa caaatcagac tgttcttttt ttatactgca
    30061 ctgtttttgc ctgtctgatt aaggggttag cgcagtattt tgtgatgata gcgattaaaa
    30121 tccctatttt tcagtcgatt aagaacagat aatattctga atttattgat agataaacag
    30181 aaaaaagtgc ctttgtcagc ataaaataac ggcacaaagg gcggaataat tcactatcat
    30241 tcaggggatt atgctggaca tttttcattc tctaatgttt taactttgta attattgctg
    30301 ttaaaaaatt aatcacctgc caaaagaaat aaaaaagaga aagcctccga ttaaattatt
    30361 tcgctacact ggttccactt ttgtgattta cacgggttac ccatgaagct gaccatcatt
    30421 cgattagaaa actttagcga ccaggaccgg attgacctgc aaaagatctg gccggagtat
    30481 tccccttcct ctttacaggt tgacgataac caccgtatct acgccgcgcg ttttaacgag
    30541 cgtctgcttg cggccgtgcg ggtaacctta agcggcaccg agggagcact ggattctcta
    30601 cgcgtgcggg aagtcacccg ccgtcgcggt gtggggcaat atctgctgga agaggttttg
    30661 cgtaacaatc ctggcgtttc atgctggtgg atggcggatg caggcgtgga agatcgcggt
    30721 gtgatgacgg cgtttatgca ggcactggga tttacggcac agcagggcgg ctgggagaag
    30781 cgttaatcgt caagttgatt tcaaaagtga tgaccaacga gaaattgcct gatgcgctac
    30841 gcttatcagg cctataatgt gtgctgcaat ttactgattt ctttggttct tgtaggccgg
    30901 ataaggcgtt tacgccgcat ccggcatgaa gcaacgtatt cgatattagc aatttggcgg
    30961 caacccaaag ttgccgatta acaattactt cgcatcggtc gccgtgccgt tggcgtgcca
    31021 gtcaaatacg ccgaactcaa agccctttag atcgcctttc tcatcccagg tcagtggccc
    31081 catcacggta tccacggagt tcgcttttag gtatttggcg atttcagccg gatcgtcaga
    31141 ctgattcagg cccgcctgca aagattgcag cgcggcgtag gtggtccaga cgaatgcgcc
    31201 gcttgggtcc tgttttttcg ctttgatcgc atcaacaatg ggtttgttcg ccggaacctg
    31261 atcgtagttc ttcggcttgg tcaccagcag cccttccgct gattcgcccg caatgttaga
    31321 cagcgaaacg ttagccacac cttccggccc cataaactga gttttcagcc ctgccgcgcg
    31381 tgcctgacgc aggatttgcc ccatttccgg gtgataaccg ccgtagtaaa cgaagtcgat
    31441 attctctttt ttcagacgcg ccaccagcgt tgagaaatct ttttccccgg cggtgatacc
    31501 atcaaagaac accacgttag cattgccttt cttcaggccg tcctgcaccg ctcgtgccag
    31561 accttcgccg tattgctgtt tgtcgtgaac gatagcaata cgctgcggtt tcactttctc
    31621 aagaatatat ttcgccgccg tcggcccctg gtcggagtcc aggccggtgg tgcgcaggat
    31681 cagctgatag ccacgggcgg tcagctccgg cgcggttgcc gctggggtga tcattaaaat
    31741 gccttcgtct tcgtagatgt cagacgcagg ctgcgttgat gaagaacaga ggtgaccaat
    31801 cacatattta atgccgtcgt taacgacttt gttcgccacc gcaaccgcct gtttcgggtc
    31861 acaggcatcg tcatatttta cgatttgcag tttgttgcct ttaatgccgc ctttagcgtt
    31921 gatatccgca accgcctgct ctgcgccggt aaactcctgg tcaccgtact gcgcaaccgg
    31981 accggacatt gcacccacga ccgcgacttt aatatcttct gccagagcca tattgctgaa
    32041 tgccagcgcg atacatcctg ccagtaacgc tttacccttt atgttcatcc tgagaatccc
    32101 cattcttctg gttattacgt gtgttgtgat gttgttgttc agcactttat ttcgttttat
    32161 gcatgactac ccgtgcttta gcagcatact ctgctaaaac atacccgatt tttatgatat
    32221 tggaatagct attttgacag tttattaaca atctgcgtgg ggattggcgt tttgccggag
    32281 ggggaattga ttattgtaga ggcccaaaaa acaaaaaccc cggactctca tccagggttc
    32341 tctgcttaat agcggaaatt acgcttcaat agcggcgcga agttttttca tcgcgttctt
    32401 ttccagttgg cgcacacgtt cagcggaaac accgtaacgg tcagccagtt cctgcaacgt
    32461 ggacttgttg tcttcgtcca gccagcgcgc acggatgatg tcctggctgc gttcgtccag
    32521 accatgcatc gcgtcggtca gacggtttgc cgcctgctct tcccagttat catcttcgat
    32581 gccgtcggca aagttagatg atttatcctg cagatagagc accggtgcca tcggctggct
    32641 gtcggaatcg tcgtcggaag acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc
    32701 catctcacgt acgtctttgc tggttacgcc cagttcacgg gccaccattt cgacttcatc
    32761 ctggttaaac cagcccagac gctgcttggt tttacgcagg ttgaagaaca gtttgcgctg
    32821 cgctttggtg gtcgcaactt tgacgatacg ccagttacgc agaacgtatt cgtggatctc
    32881 tgctttgatc cagtggacgg cgaaggagac caggcgcaca cccacttccg ggttaaaacg
    32941 gcgcactgct ttcatcaggc cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc
    33001 atagcccgca taattacgag caatatgaac aacaaaccgc aggtgagaca ggatcagcgt
    33061 tttagctgct tccagatcgc catggtaatg cagcttttca gccagcgccc gctcctcgtc
    33121 agccgacaac atcggccacg cgttagctgc ccggatgtag gaatccaggt tgccaactgg
    33181 ggctaaagct aaactttgca ttttgtcagt cattcaaatc ctctcaatcg atatcttctg
    33241 gcgcttcagt ggtagcaaca actgtgccaa agcttaagag caacgaggtt atcattcact
    33301 gttttatcag accgtgattt tatccacaag ttcaatgcaa gcttgtgaat aaattacgca
    33361 caaaatgtga catagagatg aaataccggg aagagacaac ggggtctctt tccctgctac
    33421 ggaacccatt gcagggaaag agtataacac gcttttatta ttcaggcgta aagtggcgta
    33481 aatgttgtac cgtggcaagc cacgctgcca cccagccaat catcgagcat accagcagca
    33541 atagcaggca ttcatcgaac gataaaccat tgatatcaaa cttcgttccg aaaacctgtg
    33601 ccacttccgc caccgctgac gacaaacgca gtaccagaat ttctgacaaa attaatgaca
    33661 acaatgcgcc agaaaatccc agcagcgcac cgccgtacag gaacgggcga aggatgaaac
    33721 catccgtcgc accaatcagt ttctgcacgt tgatggagtc acggcgagca aagatgctca
    33781 gacgtacgct gttgccgatg acgaggaaca ctgccgccac catcaacacg ccgatcatcg
    33841 ccgaaacgcg cccaaccagc ccggtcaacg ccgccagacg ggcaaaccag ctgtcatcca
    33901 tccgcacttc gtcaataccg ttaatctgcg tgatacgatc acgcagcgta ttgagtgatt
    33961 ccgtcccctg aaaatcgagt ttcgggatca ccaccgccac tgccggaagc gggttttctt
    34021 ccagcatatc cagcgcacca ccaaaaccag accagttacg gaactcgccc agtgcgtctt
    34081 cacgagaaag atagttcact ttctccacgc cttgctcggc ctgcaactgt gccaccacgc
    34141 ccgcagcggc gtcatcgtcc agcgtttttt gcaagtaaac agtgatttgc ggtgacggat
    34201 aatactgcgt cgccgcctga ttaacgtttt tgtacaccat ataacagacg ctgggcagcg
    34261 tcagggagat ggcgataacc atcaccgtta aaaacgtggc gaacggtttg cttttcagat
    34321 cctgcaatgc gccgtggaag gcatagcgca cctgttcgtt gaaaacgttg gttttgcgat
    34381 ttaccggttt tggcgaggat ttcgcgcgtt tcggtgcgtt acgaccgccg tcgcctgagc
    34441 cgccgaccga tttacggaag cgatccagac gcccaccaaa ctgccgaata tgattgattg
    34501 catcgcgctt attcatggcc cacgcctcca tgcaagtgac catcgctcag ggtgagcatg
    34561 cgataggaac gccgtgaaat cagattaatg tcgtgcgttg ccatcaatac ggttacccca
    34621 acgcggttaa actcttcaaa cagacgtaga atcccttccg acaacgcgtc gtccaggtta
    34681 ccggtcggtt cgtccgccag cagtaccgcg ggcttattca ccaccgcgcg ggcaatgcca
    34741 acacgctgtt gttcaccgcc ggaaagctga atagggaagt tcttcgcttt gtccagtagc
    34801 ccgactttat ccagtgccgc cgacacccgg cgacgaatat cgtcaccgct ggcaccagcg
    34861 ataatcagcg ggatcgccac gttatcgtag acagtacggt ccatcagtag atggtgatcc
    34921 tggaaaatca tgccaatctg gcggcgcaga aacggaactt cacggttttt cagacgcgtg
    34981 atgtcatggc cgctaaacca gattttcccg gcgctgggcc gctcaatccc acagatcagc
    35041 ttcaggaggg tacttttccc tgcgccggaa tgaccggtca gaaacgccat ctcacccggc
    35101 tgcatatgga acgtaacgcc ctgcagcgcc tgtctcccac cgagataagc cttgctgaca
    35161 tgttcaaagc gaatcattgt taatcctctc gggcaaaaag tgcctctata aagtcgtccg
    35221 ccttaaacgg acgcaaatcc tcaatacgtt cgccgacacc aatgtagcgg atagggatac
    35281 caaactggtc agccaccgag aaaattaccc cgcctttcgc cgtgccgtcc agtttcgtta
    35341 gcgtgatgcc ggttaagcca acagcttcgt ggaacagttt ggcctggctt accgcgttct
    35401 gcccggtgct ggcatcaata gtcagcataa cttcatgcgg cgcttcaacg tcgagtttct
    35461 tcatcacgcg gacgattttc ttcaactctt ccatcagatg cgatttgttc tgcagacgtc
    35521 cggcggtatc ggcaatcagg acgtcgatat tacgcgcttt agctgcctga atggcgtcga
    35581 agataacaga ggcggaatcc gcaccggtat gctgggcaat caccggaata ttgttgcgct
    35641 gaccccagac ctgaagctgt tcaaccgcag ctgcacggaa agtatcgccc gccgccagca
    35701 tcaccgattt accctgctgc tcaaactgac gcgccagctt accaatcgtc gtggttttac
    35761 ccacaccgtt gacgcccacc atcaggatca cgaacggcgt tttatcttca acattcagcg
    35821 gctcatcgac tttcgccaga atctcgccca tctcttcttt cagcaggcca tagagcgcct
    35881 cggcatcacg aagctgcttg cgggatgcgc cttccgtcag attggtgata attttacgtg
    35941 tggtttccac gcccacatcg gcgatcaaca gctgttcttc cagctcttca aacagatcat
    36001 cgtcgatttt tttaccgcgg aacaggctga taaatccgga accgagattt tctttggttt
    36061 ttaacaggct gcgtttcagg cgcgcgaaga agccttcttt ggtcggtttt tcttgctcct
    36121 gggcaatttc tgcaaccggc tgctcttctt ctgccggagg aactaccatc accgcctctt
    36181 ctgccgcttc ggcagccagc gcctgtgctt ccagctcttc gtcggtgatt tcttctttag
    36241 ccgcttcttc ttctgccgct tcgacaatct ctacggtttc cgcttcagcc tgccactctt
    36301 ctggcgaaac ctcttcggcg ttgacgtctt ccggcaacgg cagctcttca cgttcgatag
    36361 ccactggctc cggcgtttct tctacgaccg gttccggctg tgcaacaact tccgcttcag
    36421 gctgcgcttt ttcactttca acaacctgtt cagtgacttc cacaacgtcg gcagcaaaag
    36481 tttccgcctc ggcttcagta tgcgcctgcg gctgctcttc aacggcttgt tcagaagcct
    36541 tcgcaggctc ttgcgcctga acgatttctt ctacaaccgg ttgttcattc tgaacttctg
    36601 tctctttttc cggggtctgc tctttttgac caaagcccag ccaggaaaaa aagccacgtc
    36661 ttttttcttt cgccatttgc gactacactc ctcgctgttg cttcatggca cagcgttaac
    36721 gctatgtaca tagcagctaa aaaaatgatg aaatagtcta tcacttaact taattcacat
    36781 caccgcctgc aagtatgtgt tatctggcgg attgagcaat ttatcatgaa aaaaccgaat
    36841 cattccggca gcggccaaat ccgcattatt ggcgggcagt ggcgaggccg taaactcccg
    36901 gttcctgata gcccaggtct gcgccccacc accgaccgcg tacgcgaaac gttgtttaac
    36961 tggctggctc cggtcattgt tgacgcccaa tgtctggatt gcttcgccgg gagcggcgcg
    37021 ctggggctgg aagcgttatc gcgctacgct gcgggggcaa cgttgattga gatggatcgc
    37081 gtggtttctc agcagttaat taagaatctg gcgacactaa aagcaggcaa tgcacgcgtg
    37141 gtgaacagca acgcaatgtc attcctggcg caaaaaggca caccgcataa tatcgtgttt
    37201 gtcgatccac cgttccgccg tggcttgtta gaagagacga taaatttact ggaagataac
    37261 ggctggctgg ctgacgaagc cctgatttat gtcgaaagcg aagtcgaaaa cggtctgccc
    37321 actgttccgg caaactggtc attacatcgg gaaaaagtgg cgggtcaggt ggcttatcgg
    37381 ctgtatcaac acgaagcaca aggagaaagt gatgctgatt aatattggtc gtttgttaat
    37441 gctctgcgtt tggggatttt taatcctcaa tctggtgcat cccttcccgc gcccgctaaa
    37501 tattttcgtt aacgtggcgc tgatttttac cgtgctgatg catggtatgc agctggcgct
    37561 attgaaatcc actttaccga aagatggccc gcagatgacc accgccgaaa aggtgcggat
    37621 tttccttttc ggcgtgtttg aactgctggt ctggcagaag aaatttaaag ttaaaaaata
    37681 accttattgt tcgccaacaa agctaacaaa gcgcgttcct ttatagctca gcgtaccttt
    37741 atcgcccact gtcagggcgt ggtactgctg ggcgtcgagg cgaaacgtct gctccattcc
    37801 tccgctttgc ggtttgaagc ttgcctcata gcgcatactg gtgcctgccg gagtcacttc
    37861 ctgctggcgc gaacggcgat cgttaatcgg tttttcccgc ttgttgctca ccaccaccag
    37921 cttttgctgg agcggagcca tatcattatc agctttttcc cgtcgctgtt gcataaaacg
    37981 aaacgatgcg gcgacgacaa ttaagccaat gataacaata aagaaaagag gtggtttact
    38041 catctttatc cctcatcgga aaatgcggaa ttaagcatac cctgccagtt atggtgttgt
    38101 catccgtcca ccctcgccac taaactggaa gcaagaccgt aggcattctg cttacgaaaa
    38161 aataacgaat tcaaggaact aagatgcttt ggtcgtttat cgctgtctgt ctttccgcat
    38221 ggctatctgt ggatgcatcg tatcgtgggc caacctggca acgctgggtg tttaaaccgt
    38281 taacccttct tctcctgctg ttactggcct ggcaagcacc gatgttcgac gccattagct
    38341 atctggtgct ggcgggactg tgcgcctcac tgctgggcga tgcgctaacc ctgttgccac
    38401 gtcaacgtct gatgtacgcc atcggcgcgt ttttcctctc gcacctgctg tacaccatct
    38461 atttcgccag tcagatgacg ctctctttct tctggcctct gccactggtg ttgctggttc
    38521 tgggtgcgct gctactggcg attatctgga cgcggctgga agagtaccgt tggcctatct
    38581 gcacgtttat cggcatgacg ctggtgatgg tgtggctggc aggtgaactg tggttcttcc
    38641 gtccgaccgc tccggcgctc tctgcgtttg ttggcgcttc gttgctgttt atcagtaact
    38701 ttgtctggct ggggagccac tatcgccgac gcttccgtgc ggataacgcg attgctgcgg
    38761 cctgctactt tgccggtcac ttcctcatcg tccgctcact gtatctctga taaaacttga
    38821 ctctggagtc gactccagag tgtatccttc ggttaatgag aaaaaactta accggaggat
    38881 gccatgtcga cacctgacaa tcacggcaag aaagcccctc aatttgctgc gttcaaaccg
    38941 ctaaccacgg tacagaacgc caacgactgt tgctgcgacg gcgcatgctc cagctcgcca
    39001 acgctctctg aaaacgtctc cggcacccgc tatagctgga aagtcagcgg catggactgc
    39061 gccgcctgtg cgcgcaaggt agaaaatgcc gtgcgccagc ttgcaggcgt gaatcaggtg
    39121 caggtgttgt tcgccaccga aaaactggtg gttgatgccg acaatgacat ccgtgcccag
    39181 gttgaatctg cggtgcaaaa agcgggctat tccctgcgcg atgagcaggc cgccgacgag
    39241 ccacaagcat cgcgcctgaa agagaatctg ccgctgatta cgcttatcgt gatgatggca
    39301 atcagctggg gtctggagca gttcaatcat ccgttcgggc aactggcgtt tatcgcgacc
    39361 acgctagttg ggctgtaccc gattgctcgt caggcattac ggctgatcaa atccggcagc
    39421 tacttcgcca ttgaaacctt aatgagcgta gccgctattg gtgcactgtt tattggcgca
    39481 acggctgaag ctgcgatggt gttgctgctg tttttgattg gtgaacgact ggaaggctgg
    39541 gccgccagcc gcgcgcgtca gggcgttagc gcgttaatgg cgctgaaacc agaaaccgcc
    39601 acgcgcctgc gtaacggtga gcgggaagag gtggcgatta acagcctgcg gcctggcgat
    39661 gtgattgaag tcgccgcagg tgggcgtttg cctgccgacg gtaaactgct ctcaccgttt
    39721 gccagttttg atgaaagcgc cctaaccgga gaatccattc cggtggagcg cgcgacgggc
    39781 gataaagttc ctgcaggagc caccagcgta gaccgtctgg taacgctgga agtgctgtca
    39841 gaaccgggtg ccagcgccat tgaccggatt ctgaaactga tcgaagaagc cgaagagcgc
    39901 cgcgccccca ttgagcggtt tatcgaccgt ttcagccgta tttatacgcc agcgattatg
    39961 gccgtcgccc tgctggtaac gctagtgccg ccgctgctgt ttgccgccag ctggcaggag
    40021 tggatttata aaggtctgac gctgctgctg attggttgcc cgtgtgcgtt agttatctca
    40081 acgcctgcgg cgataacctc cgggctggca gcagcagcgc gtcgtggggc gttaattaaa
    40141 ggcggtgcgg cgctggaaca actaggtcgt gttacccaag tggcgtttga taaaaccggt
    40201 acgctgaccg tcggtaaacc gcgcgttacc gcgattcatc cggcaacggg tattagtgaa
    40261 tctgaactgc tgacactggc ggcggcggtc gagcaaggcg cgacgcatcc actggcgcaa
    40321 gccatcgtac gcgaagcaca ggttgctgaa ctcgccattc ccaccgccga atcacagcgg
    40381 gcactggtcg ggtctggcat tgaagcgcag gttaatggtg agcgcgtgtt gatatgcgct
    40441 gccgggaaac atcccgctga tgcatttgct ggtttgatta atgaactgga aagcgccggg
    40501 caaacggttg tgctggtagt acgtaatgat gacgtgctgg gtatcattgc attgcaggat
    40561 accctgcgcg ccgacgctac aactgccatc agtgaactga acgcgctggg ggttaaagga
    40621 gtgatcctca ccggcgataa tccacgcgca gcggcggcaa ttgccgggga gctggggctg
    40681 gagtttaaag cgggcctatt gccggaagat aaagtcaaag cggtgaccaa gctgaatcaa
    40741 catgcgcccc tggcgatggt cggtgatggt attaacgacg cgccagcgat gaaagctgcc
    40801 gccatcggga ttgctatggg tagcggcaca gacgtggcgc tggaaaccgc cgacgcagca
    40861 ttaacccata accacctgcg cggcctggtg caaatgattg aactggcacg cgccactcac
    40921 gccaatatcc gccagaacat caccattgcg ctgggactga aagggatctt cctcgtcacc
    40981 acgcttttag ggatgaccgg gttgtggctg gcagtgctgg cagatacggg tgcgacggtg
    41041 ctggtgacgg cgaatgcgtt aaggttgttg cgcaggagat aaggcaaacc gatcgcaaca
    41101 ttgagcgcga tcggtcccct cgcccctctg gggagagggt tagggggtga ggaaaaggcg
    41161 gcatcgaagc caatcagccc ctatcaaccg cctttacgaa tcaaataacg ataaggcagt
    41221 ccatccgtct ctttcgcaac cagttcgtgt tccataaagg tacaaaaccc aggaatatcg
    41281 cgggtagtgg ccggatcgtc ggcgataatc agcaacgttt cgccaggctg catattgcgc
    41341 acggttttgc gcaccatcat caccggttcc gggcagcgca ggccaagcgc gtcgagtgtg
    41401 tggtcagggc tggaaaagag atcggtcatt ttcttctcat cacttaaaaa aacggcgcta
    41461 gtttacgccc tgtgagtccg taagcaaacc aggttaacga ttgcgtgaaa attaaccatt
    41521 gcattgtcaa cgtaaagcag tatcatgcgg cggctcgaaa aaagggtaag cacgttatta
    41581 tgttaaggta acagacgtgt cgtacgtatt gggttccctc accccaatgg ttaatcaaaa
    41641 aggtacaata tgaacgtttt ctcgcaaact caacgctata aggcgttgtt ctggttatcg
    41701 ttatttcatc ttctggtgat cacctccagt aactatctgg ttcagcttcc cgtctccatt
    41761 tttggtttcc ataccacctg gggcgcgttt agctttccgt ttatttttct tgctaccgac
    41821 ctgaccgtgc gtatttttgg cgcaccgctg gcccgacgca ttatcttcgc ggtaatgatc
    41881 cctgcgttat taatctccta cgtcatctcg tcgctattct atatgggttc ctggcaggga
    41941 ttcggcgcac tcgcccactt caatctgttt gtcgcccgta tcgccaccgc cagtttcatg
    42001 gcctacgcgc tggggcaaat cctcgacgtg cacgttttta accgcctgcg tcagagtcgc
    42061 cgctggtggc tggcaccgac agcgtccaca ctgttcggta acgtcagcga cacgctggcc
    42121 tttttcttca ttgccttctg gcgtagcccg gatgccttta tggctgaaca ctggatggaa
    42181 atcgcgctgg tcgattactg tttcaaagtg ttaatcagta tcgttttctt cctgccaatg
    42241 tatggcgtat tactcaatat gctgttgaaa agactggcag ataaatccga aatcaacgct
    42301 ttgcaggcga gttaaaggtt cgttatcaga gttgtgataa gatggatgaa tgagccgtta
    42361 tggccgttta tcgaaaggaa gaagtcaatg cgcaatctgg ttaaatatgt cggaattggc
    42421 ctgctggtta tggggcttgc ggcctgtgat gataaagaca ctaacgctac ggcgcagggt
    42481 tcggttgcgg aaagtaacgc taccgggaat cccgtcaacc tgcttgatgg caagttaagt
    42541 ttctcgctgc cagcggatat gaccgaccag agcggtaagc tgggaacgca ggccaataac
    42601 atgcatgtct ggtccgacgc caccgggcag aaagcagtca tcgtcatcat gggcgatgat
    42661 ccgaaagaag atctggcggt gttggcgaag cgtctggaag atcagcaacg tagccgcgat
    42721 ccgcagctgc aagtggtaac caataaagcc attgagctga aaggtcacaa aatgcagcag
    42781 ttagacagta ttatctccgc gaaaggccag acggcgtact cttccgttat tctgggtaac
    42841 gtgggtaatc aactgctgac catgcaaatt acgctgccag ctgacgatca gcaaaaagcg
    42901 caaaccaccg cagaaaacat cattaatacg ctggttattc agtaagtttt aagatgatga
    42961 ggcggcctca gggacgtgtt ccggaggccg tttttttaat cgccacgtca gtaataacgc
    43021 gattgcgacc agtcccgccg ccgccagata aatcaccggt acgcccgccc agctcatcac
    43081 cagcccagcc agtggtccag tcacgccaag cgataaatcc ataaatacgg tgtaagttgc
    43141 cagcgccgcc ccctgatttt gctgcggaac cgcttttacc gccactacac ccaatgccgg
    43201 gaacaccagc gaaaacccgg cccccgccag taagacgccg attttcgcca tccacggcat
    43261 agtcgccacg ccaaccagta gcaggccgat tatctcaacg ctaaagcaaa tcatcgctac
    43321 gtttaagcca ccgatacggt taatgccgtt agggaataac aaacgcgtac cgacaaacgc
    43381 acagctaaac agcgtcagcg cgaaagccgc accgtcccaa cctttagcgt cataaaacag
    43441 cgtgataaag gtggcgatga cgccaaatcc ggcggaagcc agtgccagcg ccataccgta
    43501 cagccagacg cgcccaagca ccgcgcgaaa cggcagcggt ttgcctttac tggcttttac
    43561 cgtcggacgc gggatcgcca acaaaatggc caccagcgcc acgcccataa tgattaacgc
    43621 taacgcctgc aagccgcccc agtgataaaa cacgacgcct aacggcgcac ccatcgccat
    43681 cgccccgtaa gtgacaatgc cgttccacga aatcacccgc ccgatatgca gcgagccaac
    43741 cactccaacg ccccataggg tcgatcccgt tccggcaaaa ctttgcccaa tcccaaggat
    43801 gacgcgcccc aggcaaagta ataacaggct gatgacaggc agactggcgg ttaatcctgc
    43861 cgtcagatac cccagaccgc tcaaaaagca gccgcataaa ccgaagacga caatcttttt
    43921 gggtcccagc aaatcggcgt aacgtccggc atgagggcgg ctcagcaagg tggcgaaata
    43981 ttgcaggctg ataaccaatc ctgcccagaa ggcgctaaag cccatcacat catggacata
    44041 gcccggtaat acagcgagcg gcaacccgat ggtgaggtag ctggcgaagt taaacatgac
    44101 aatggagaca atgcgcaaat tcaggcgcaa tccgtttagc gcgggttcgg ctacgggttc
    44161 gggcatgagg atcaccacat ttttacaaca gtgtttcatt tttaccatgt gctgacgtga
    44221 aaatcagcag taagaatcag aatattgctg gcgtgactcc cgcgctacac ttaacacaaa
    44281 aagtcacaag gaagccccaa tggaaacccc tcaacccgat aaaacgggca tgcacattct
    44341 gctcaagttg gcctcgctgg tagtgatcct cgcgggcatt cacgcagcgg cagatatcat
    44401 tgtgcagctg ttactggcgc tgttttttgc catcgtcctc aacccgctcg ttacctggtt
    44461 tattcgtcgg ggagtacaac gccccgttgc cattacgatt gtagtggtgg tgatgctgat
    44521 cgcactaacc gcgctggtcg gcgtactggc ggcatcgttt aacgaattta tctctatgct
    44581 gccgaagttt aataaggagc tgacgcgcaa actttttaaa ttgcaggaga tgttgccttt
    44641 tcttaatttg catatgtcgc cggagcgaat gctgcagcgg atggactcgg aaaaagtggt
    44701 taccttcacc acagcgctaa tgaccgggct ttccggggca atggcgagcg tgcttttgct
    44761 ggtgatgacc gtagttttta tgctgtttga agtgcgccac gtcccttaca aaatgcgttt
    44821 tgcgctgaat aacccacaga ttcacatcgc aggattacac cgcgcactta aaggcgtttc
    44881 gcattatctg gcgttaaaga cgctactcag tttatggaca ggtgtcatcg tctggctggg
    44941 gctggcgcta atgggcgtac agtttgcgct gatgtgggca gtactggcgt ttttgctcaa
    45001 ctacgtgccc aatatcggcg cggtaatttc cgccgtaccg ccaatgattc aggtgctgct
    45061 gtttaatggt gtttacgaat gtattctggt cggcgcattg tttttagtgg tccatatggt
    45121 catcggcaat attttagaac cacggatgat gggccatcgc ctggggatgt ccaccatggt
    45181 ggtatttctt tcattgttaa tctggggatg gctgctcggc ccggtaggga tgctactttc
    45241 ggtgccatta accagcgtgt gtaaaatctg gatggaaacg acgaaaggtg gcagcaaact
    45301 ggcgatttta ctggggccgg gcagaccgaa aagtcggtta ccgggatgag gcgacaagtg
    45361 atacgatacg cactttcatt ttccattaaa cgttggccct gatatgtatc ggatagttct
    45421 ggggaaagtt tcgaccttaa gcgcagctcc actgccaccg ggtttacgcg agcaagcacc
    45481 gcaaggtcca cgacgcgaac gctggctggc ggggcgtaca ttgctttcgc acacgctttc
    45541 cccgctaccg gagatcatct atggcgaaca aggcaaacct gcattcgcgc cggaaacgcc
    45601 gctatggttc aacttaagcc atagcggtga cgatatcgct ctgctgttga gcgatgaagg
    45661 cgaagtcggc tgcgatatcg aagtgattcg cccgcgcgtc aactggcgct ggctggcgaa
    45721 cgccgtgttc agcctcgggg aacacgctga aatggacgcc gtgcatcctg atcagcaact
    45781 ggaaatgttc tggcgcatct ggacgcgcaa agaagccatc gttaaacagc gtggcggcag
    45841 cgcctggcaa atcgtcagcg tagatagcac ctatcactcc tcgctgtcag tcagccattg
    45901 tcagctagaa aatttaagcc ttgcgatctg cacccccacc ccctttacgc tcaccgccga
    45961 cagtgtgcaa tggatcgatt cagttaactg atccgcccac ccgactgccc atctattgat
    46021 ccagaacagg taatcagtat gacgaatact taaaatcgtc atacttattt ccgccatcta
    46081 ttttaatcca ttggggttac catgctctcc acactccgcc gcactctatt tgcgctgctg
    46141 gcttgtgcgt cttttatcgt ccatgccgct gcaccagatg aaatcaccac cgcctggccg
    46201 gtgaatgtcg ggccactaaa cccgcacctt tacacgccta accagatgtt cgcccagagc
    46261 atggtttatg aaccattggt gaaatatcag gcagacggtt cggtgatccc gtggctggca
    46321 aaaagctgga ctcattcaga agatggtaaa acctggacct tcaccctgcg tgatgacgtg
    46381 aaattctcca acggtgaacc gttcgatgcc gaggcggcgg cagaaaactt ccgcgcagtg
    46441 ctcgataacc gtcaacgtca cgcctggctg gagctggcaa accagattgt tgatgttaaa
    46501 gcactcaata aaacagagct gcaaattacc ctgaaaagcg cctactatcc tttcctgcaa
    46561 gaactggccc tgccccgtcc tttccgtttt atcgctccct cgcagtttaa aaaccatgaa
    46621 accatgaacg gaattaaagc gccgattggc accggaccgt ggattttgca ggaatcgaaa
    46681 ctgaatcagt acgatgtctt cgtccgtaac gaaaactact ggggcgaaaa gccagcgatt
    46741 aaaaagatca cctttaacgt catcccggac ccgactaccc gcgcggtggc gtttgaaact
    46801 ggcgatatcg acctgctgta cggaaacgaa gggttattac cgctcgatac cttcgcccgc
    46861 tttagccaga atccggctta ccacacccaa ctgtcacagc cgatcgaaac cgtgatgctg
    46921 gcgctcaata ccgccaaagc ccccaccaac gagctggcag tacgtgaagc tcttaattac
    46981 gcggtaaaca aaaaatcgct gattgataac gcgttatatg gcacccagca ggtcgccgac
    47041 accctgtttg ccccttctgt gccttacgct aatctcggcc tgaaaccgcg ccagtacgat
    47101 ccgcagaaag cgaaagagtt gctggaaaaa gccggttgga cgctgcctgc gggcaaagac
    47161 atccgcgaga aaaatggtca gccgctgcgc attgaactgt cgttcatcgg caccgatgcg
    47221 ttaagcaaat cgatggcgga aatcattcag gctgatatgc gccagattgg cgcagatgtc
    47281 tcgctgattg gcgaagaaga gagcagtatc tatgctcgtc agcgcgacgg tcgttttggc
    47341 atgattttcc accgcacctg gggcgcgcca tacgatccac acgccttcct gagttcaatg
    47401 cgcgtaccgt cgcacgctga cttccaggca cagcaaggat tagccgacaa gccgctgatt
    47461 gataaagaga tcggcgaagt gctggcgacc catgacgaaa cgcaacgtca ggcgctgtat
    47521 cgcgacattc tgacccgtct gcatgacgag gcggtttatc tgcctatcag ctacatctcg
    47581 atgatggtgg tatcaaaacc ggagctgggt aatatcccct acgcgccgat cgccaccgaa
    47641 attccgttcg aacagattaa accggtgaaa ccttaatgtt gcgttacgta ttacgccgct
    47701 ttctgctgct gatcccgatg gtgctcgccg cctcggtgat catttttctg atgctgcgcc
    47761 tcggtaccgg cgacccggcg ctcgattatc tgcgtttatc taacctgccg ccgacgccgg
    47821 agatgctggc ctctacccgc accatgctcg ggctggatca gccgctgtac gtccagtacg
    47881 gtacctggtt gtggaaggcg ctgcatcttg actttggtat ctcattcgcc agccaacgcc
    47941 cggtactgga cgatatgctg aacttcctgc ccgccacgct ggaacttgca ggtgcggcgc
    48001 tggtattaat tctgctcact tccgtaccgc tcggtatctg ggcggcgcgc caccgcgatc
    48061 gtttgccgga tttcgccgtg cgctttatcg cgtttcttgg cgtgtcgatg cccaacttct
    48121 ggctggcatt tttgctagtg atggcgtttt cggtgtatct gcaatggcta cccgcgatgg
    48181 gctacggcag ctggcagcac atcattttac ctgcggtttc catcgccttt atgtcgctgg
    48241 cgattaacgc gcgactgctg cgcgccagta tgctggacgt cgccggtcag cgtcacgtca
    48301 cctgggcgcg gctacgcggc ctgaatgaca aacagaccga acgccgccat attctgcgca
    48361 atgcctcgct gccgatgatc accgccgtgg ggatgcacat tggcgaattg attggcggga
    48421 cgatgattat cgaaaacatc tttgcctggc ctggcgtcgg gcgctatgcg gtgtcggcga
    48481 tttttaaccg tgactatccg gtgattcagt gctttacgct gatgatggtg gtggtttttg
    48541 tggtctgtaa tttgattgtc gatttgctca acgccgcgct ggacccgcgc attcgtcgtc
    48601 atgaaggagc gcacgcgtga actttttcct ctcttcccgc tggtcggtac gcctggcgct
    48661 gatcattatc gccctgctgg cgctgattgc gctcaccagc cagtggtggc agccgtacga
    48721 tccacaggca attgatttgc cgtcgtgcct gctttcgccg gatgcgcagc actggctggg
    48781 caccgatcac ttaggtcgcg atattttctc gcggctgatg gcagcgaccc gcgtgtcgct
    48841 cggttcggta atggcctgcc tgctgctggt gctgacatta gggctggtta ttggcggcag
    48901 cgccgggttg attggcgggc acgttgatca ggccaccatg cgcgtcgccg atatgtttat
    48961 gaccttcccg acctcgattc tgtcgttctt tatggttggc gtgctcggca ccgggctgac
    49021 caacgtaatt atcgccatcg ccctgtcgca ctgggcgtgg tatgcacgca tggtgcgcag
    49081 cttggtgatt tcactacgcc aacgcgagtt tgtgctggcg tcacggcttt ccggtgcggg
    49141 ccatgtgcgg gtgtttgtcg atcatctggc aggcgcggtg atcccttcgc tgctggtgct
    49201 ggcaacgctg gatatcggcc atatgatgct gcacgtcgcg gggatgtctt tccttggcct
    49261 cggtgtgacc gcgccgaccg ccgaatgggg cgtgatgatt aacgacgcgc gccagtatat
    49321 ctggacccag ccgctgcaaa tgttctggcc ggggctggcg ctgtttatca gcgtgatggc
    49381 ctttaacctg gtgggtgacg cactgcgcga tcatctggat cctcatctgg tgacggagca
    49441 cgcacactaa tgccgcaaca gattgaacta cgtaatatca cgctacaggc cgcgcagccg
    49501 ctggtacacg gtgtatcgtt aaccctgcaa cgcgggcgcg tgctggcgtt agtcggcggt
    49561 agcggcagcg ggaaatcatt aacctgcgcc gcgacgctgg gcattttgcc cgctggcgtt
    49621 cgccagacgg cgggggaaat tttagccgat ggcaaaccgg tttcgcctta cgccctgcgc
    49681 ggcatcaaaa ttgccaccat catgcagaac ccgcgcagcg cctttaatcc actgcacacc
    49741 atgcacaccc acgcgcggga aacctgcctg gcgttaggga aacccgccga tgacgctacg
    49801 cttaccgctg ccatagaagc ggtggggctg gaaaacgccg cgcgcgtgct gaagctgtac
    49861 ccgttcgaga tgagcggcgg catgttgcag cgcatgatga ttgcgatggc ggtgctgtgt
    49921 gaatcaccgt ttatcatcgc cgatgaaccg accaccgacc tcgacgtggt agcacaggcg
    49981 cgcatcctcg atctgctgga aagcattatg caaaaacaag cgccgggaat gctgctggtc
    50041 acccatgata tgggcgttgt ggcgcgtctg gcggatgacg tggcggtgat gtcacaaggt
    50101 aaaattgtcg aacagggcga tgtagaaacg ctgtttaacg cccccaaaca tacggtgacg
    50161 cgcagcctgg tttccgctca tctcgccctc tacggtatgg agctggcatc atgaccttac
    50221 ttaacgtctg cggcctttcc catcactatg cccacggtgg atttagcgga aaacatcaac
    50281 atcaggcggt gctgaataac gtttccctga ccctgaaaag cggcgaaacc gtcgccctgc
    50341 tgggacgcag cggctgcgga aaaagcaccc tcgcgcggct gctggtaggc ttagagtctc
    50401 cctcacaagg gaatattagc tggcacggcg aatcgctggc gaaacttaat cgcgcccaac
    50461 gtaaagcctt ccgccgtgat attcagatgg tgtttcagga ctccatcagt gccgtgaatc
    50521 cgcgcaaaac cgtgcgcgag atcctgcgtg aaccgatgcg ccacctgcta tcactgaaaa
    50581 aatccgaaca actggcgcgc gccagcgaaa tgctgaaggc ggtcgatctc gatgacagcg
    50641 ttctcgacaa acgcccaccg cagttaagcg gcggccagct ccagctcgtc tgcctggcac
    50701 gcgcgctggc ggtcgaaccg aaattgctga ttctggatga agccgtttct aaccttgatc
    50761 tcgtgttaca ggcgggcgtc attcgcctgc tgaaaaagct acaacaacag tttggcactg
    50821 cctgcctgtt catcactcat gacttacgcc tggtggaacg cttttgccag cgagtgatgg
    50881 tgatggacaa cggacaaatc gccgaaaccc aggcggtggg agataaatta accttttcct
    50941 ctgacgccgg acgtgtgcta caaaacgcgg tattacccgc attccccgtg cgccgtcgca
    51001 cctcagaaaa ggtttaacgc caatgcaacg agtcaccatc acgcttgatg acgatttact
    51061 ggagacgctg gacagcctga gccagcgtcg tggttataac aaccgttccg aagctatccg
    51121 cgatattctg cgtagtgccc tggcgcagga agctacccag cagcacggca cacaaggttt
    51181 cgcggtgctg tcgtatgtgt atgaacacga aaaacgcgac ttagccagcc gtattgtttc
    51241 cacccagcat catcaccacg acctctccgt cgccacgcta catgtgcaca tcaaccacga
    51301 cgactgtctg gaaatcgccg tgttgaaagg tgacatgggt gacgtgcagc attttgccga
    51361 tgacgttatc gcccagcgcg gcgtgcggca cgggcatttg cagtgcctgc cgaaggaaga
    51421 ttgagttatt tctaaaacaa ggaaagaaaa gatattaact gaacttaatt tataaaatta
    51481 agttcagttg catagataga tcctgtaaag gcagatattc tgcgtaatgt acgtcgctaa
    51541 aatttataaa tgattcaaac gctcattcag tgtttcaata atataggtat taacagagac
    51601 ttgttgctgc gcagccgcat tattaaggcg ttcactcagc gattctggat agcgtaacgt
    51661 aaaggtctta atcttttctg tacgcgcata cggctcaata cccgcagctt tacaatcctc
    51721 aagatactct cgcagggaaa gctctccttc tttttgcagc ccctgaatac tgtcagagac
    51781 aaaatcacaa taacctgaca agcccaggaa tttgccacga aatgcattca gttctggcac
    51841 gtaggtaata acagctggtt gcccggcaat ctccatagag tttggcgtct tcagtttgat
    51901 catggtttta cccctatact tagtaaccag tcacacacgc tttctacagc gcctttatcg
    51961 gtatctggcg acggatgagg ccgatggaaa cacgcaacgc tcatatttag tatgaacttg
    52021 caacgcgaac ctcttccttc cttaatttct ccgcctaatg ctttaaccag tgactctata
    52081 tcagaccact tgatcccttg aggaacagga gttttaaata tttgatccaa tgtatttttt
    52141 tgtttcttcc gtaaagcctg taccttggtt ctcatggcat gtccttatga cttcacttta
    52201 tgacatcaca gtatgaagtc aagtttccct tccttacgcc attgtcccaa tcgtcttcct
    52261 gaatcgcagc agtgcgatgg tgaaaaacgc gccgccaatt gccatcagcg tcaggaactg
    52321 cggccagacg atttcgaatc cggcaccccg gtagaggatg gcctgcgcga ggctaacaaa
    52381 gtgtgtcgtc ggcatggtca gcataatgtc ctgcaccatc tgcggcatac tttcgcgcgg
    52441 cgtggatcca ccggaaagca tttgcagcgg cagcagcacc agaatcacca gcagccccag
    52501 ttgcggcatt gaacgcgcta tcgtccccat aaaaatgccg attgacgtgg tggcaaacag
    52561 actgagcgcc acgcccagca taaacagcgg gatcgagcct tcaatcggta cgcccagtac
    52621 acctttcacc atcagcacca gcgataatcc cgataccacc agcaccacca gccccatcga
    52681 ccagatcttc gccatcatga tctcaaacgg cgttatcggc atcaccagta aatgttccac
    52741 cgtgccgtgt tcacgctcgc ggatcagcgc cgatccggtc aatacaatcg ccagcatggt
    52801 aatgttgttg atgatcgcca tcaccccgcc aaaccacgcg ggatcaaggt tcgggttaaa
    52861 gcgcatccgg gtttccagcg ataccaacgg ttcgctgtta tcacggtagc gcgcgacaaa
    52921 gctgttcact tcaccgttga taatattctg gatataccca ttgccggtaa atgcctggct
    52981 catgcgcgtg gcatcgacgt tcacctgaat atccggctgg cgtccggcga ggacatcacg
    53041 ctgaaaatta ggcggaatat ttatcgcgaa ggtatagcgt ccggcgtcca gtccggcatc
    53101 catctcatcg gcggtgatca tctccggtgg caaaaaccac ggacgataga agctgttaac
    53161 gatccggttc gataactgcg attgatccat atcggcaatg gcgatcggcg cgaggttcaa
    53221 cgatcctggc gtaacggtcg ctgacgaata caccgacacc gtaaacgaga agacaatcag
    53281 cgtcagcatc gctttatcac cgagcagact gcgcaactct ttgataccca gattaaaaat
    53341 attgcgtaaa tggcgcatca tccctcctgt tttttcagca gcaggatgct taagcccatc
    53401 accagcggga tggctatcag caacgggata aaaagttgcc acaaatcagt cagatccagc
    53461 gctttcgaga acgttccgcg ggcgatagtc agaaaatgac tggtcgggta aacctcgccg
    53521 atccaacgtc caggcccttc cagcgaagct accggatcga tcatcccgga aaactgtgtc
    53581 gccgggatca atgtgataat cgccgttccg aaaatggcgg caatctggct tttcataaag
    53641 gtggagatca gcagccccat tccagtggca atgatgatat acagcagcgc cgccaaggtg
    53701 agcgtcagga aactgccttt atgcggtacg ccaaacacaa acaccgacag gccgcagagc
    53761 aggaaaaagt tcagcatccc cagcgcgatg tacggcagct gtttgccaag caaaaattcg
    53821 ctacgggtgg tgggggtcac gtaaaggttg ataatcgacc ctagctcttt ttcccgcacc
    53881 acgctaaggg cgcttagcat tgacgggatc atcatcagca gaagcgggat caccgccgga
    53941 acaatcgctg gcaggctttt tacgtccggg ttatagcgat aacgcgtctc aatgttcatc
    54001 agcccgcttt ggctggcggg tgtcgattgt cggctcgcca catcctgaag ccaactctgg
    54061 tgcattgcct gcacgtaacc ttttaccgtt tcggcacgac tgggcatcgc tccgtcgatc
    54121 cagacgccga gttccacagg cgtaccacgc gcgatatcgc gcccgaaatt gggcgggatc
    54181 tcaatggcca ccgtgatatc gcccgcacgc atccgacgat caagctcgtc ataactggtg
    54241 agcggcggct gttcgataaa gtaacgggaa ccggagaggt tgagtgtcca cgcctggcta
    54301 ctgacggtct ggtcgcggtc gagcaccgca aagcgcaggt tttccacatc catactgatg
    54361 ccgtaaccca ttatcagcat caggatcacc gttcccatca gcgccagcgt cgaacgtact
    54421 ggatcgcgtc gcagttccag cgcttcgcgg cggctgtagc taaacagacg gcgcaggcta
    54481 aatccctgac gcggcgcgtg ggtggtgtcg tgtaccacgg gcggcgcttc ggcttcgttg
    54541 ctctgccctg ccgcttcctg caaataggcg ataaatgcct cttccagact ggcggctccg
    54601 cgtttctcaa ccagttcctg cggtgtaccg ctggcaagca cttttccggc gtgcatcagt
    54661 gagatgcggt cgcaacgttc cgcttcgttc ataaagtggg tggagatgaa gatagtcact
    54721 ttgtcctggc gcgagagatc gaccatcaac tgccagaaca tatccctcgc caccggatcg
    54781 acaccagaag taggctcatc gaggattaac atctccgggc gatgaatcac cgccaccgcc
    54841 agcgaaagcc gctggcgaat gccgagcggc aatgactccg gcagaacatc ttcaacgtcg
    54901 ttgagcttaa aacgctcgct catttcagcc actcgcgcgg gaatttccgc ttccgggatg
    54961 tgaaacaaac gggcatgtaa ctccaggttt tgccgcacgg tgagttcgtt atagagcgaa
    55021 aacgcctgcg acatatagcc cacacgacgg cgggtatcga tatcttttgg atcaaccggt
    55081 tgcccaaata gccacgcctc accttcgctg gcgggcagca gtccggtaag cattttcatg
    55141 gtggtggatt taccgcagcc gttcgaacca aggaatccaa aaatctcccc gcgtggaatg
    55201 cggaaattga cgtgatcaac ggcaacgaag gaaccaaaac gcatggtcag atcgcgcgct
    55261 tcgatggcaa tctctgcgtt ttcaggttga tacggtggga tcactaccgc ctgatgcgcc
    55321 tggcgttgcg cttgcggtaa cagattaata aatgcttctt ccagcgtggc gctttgcgtt
    55381 tgctgccgca gctcttcggc gctgccggtt gccagcactt ctccggcatt catcgctacc
    55441 agccagtcga agcgttcggc ctcttccata taggcggtgg cgaccagcac gctcatattg
    55501 ctctgccgct ggcgaatact gtcgatcaga tcccagaact gggcgcggga gagcgggtca
    55561 acccccgttg ttggctcatc aaggatcaac agttccgggt cgtggattaa cgcgcagcac
    55621 agcccaagtt tttgcttcat cccgccggag agtttacctg ccggacgatc gcgaaacggt
    55681 gctaacccgg tgctggtcag cagctcatta attcgcgctt cccgctccgc tttatcgtga
    55741 ccgaacaggc gagcgaaaaa atcgacattt tcatacaccg acaaggtgtg gtagaggttt
    55801 ttgcccagcc cctgcggcat ccaggcgatg cgcgggcaga cgtctcggcg atgcttcggg
    55861 tcgtgcatat cgccacccag caccaccaca ttgccctgct caatgacgcg ggcaccggaa
    55921 atcagtgaca ataaactcga cttcccgacg ccatccggac caatcagccc gaccatgcaa
    55981 cgggccggaa tatcgagagt gatattgttc agcgcaacgg tttttccata atgctggctc
    56041 acgcccgcca gttgcgcgac aggcgggacg ggaaccagtt ccagatgcgt cattgcggca
    56101 acctcaccac gaggtcgtca ggccacggaa gttcttcatt cacccgcacc cacgctacgc
    56161 ccggtaaacc ggttttgaca tattccagat gctgctggag taattccggt gggatacgcg
    56221 ctttgacgcg gaacatcagt ttcagccgtt catcgctggt ttcgacggtt tttggcgtga
    56281 actgggcgac actggcgaca aaactgatgg ttgcaggaat acgcagatct ggcgcggcat
    56341 cgaggatcag ccgggcttca ccgcccagtt tcagcgtgcc cgcctgttcg gttggcagga
    56401 agaaggtcat atagacgtcg ctgagatcga ccatattcag cacccgaccg cctgccgcca
    56461 gcacttcccc tggctcggca acccgatact gcacgcgtcc gtcacgcggg gctttcagtt
    56521 cactgtcatc gatatctgcg gcaatgcgcc gttcagtggc ttgcgctgct tcgacccgag
    56581 tttgcgcctg aatgatattg gtgcgtgctg cttctatagc cgctttagaa gccgaaacct
    56641 gggctttcgc tgattccagc gcagctcggg cgctctcagc ggcggcgcgg tcgtcatcca
    56701 gctgttgcgc agaaatagcc cctcgttggg cgagcgagcg ggaacgcgta tgacgtttgg
    56761 caacagagtc cagttcggcc tggcgttgat tgaccagcga ctgtgcggca cgagtttcac
    56821 tttgtcgttg ctccagcaaa gcctgcgcgg cggcaacggc gctttgtgcc tctttgattt
    56881 gcgcgatggc ttccagtcgc tgttcctgca acacgcgagt atccatcttc gccagcactt
    56941 cgccttcgcg aacaaactgg ccttctttca ccagaatggt gtcgatacgc ccggcaattt
    57001 tgctggcgat atccacttcc gtcgcttcaa tgcgcccatt gctgacagca aagccttccg
    57061 gcacacccgc cgggcgcaac agccaccacg ccacgacggc agccaccgcc agtgccccga
    57121 caacccacca cgccagatgg cgcttactct tatccataat cgacccgcca taatccctgt
    57181 aagcaaacga cgcgtgttac aactgccgcc gaacgacgtt cagcggattc ttccatccgg
    57241 gataagaagg aaacgacaaa tacgaccagc gcgcagagca ttgagcgcca acggggaagg
    57301 cttagtgaaa acgggtagta atgttgtcat catcagcatt cctggccgta aatgaaacac
    57361 gcatactcat aagtgtgatc tataaaactg gctgacaaaa cggtatccat cctgttggat
    57421 cgcatttcac gcctgttcag ttgcaggaac agagcattcc tttttacgtt ttatcaacag
    57481 tctcaaccag atagtaacat taatattcat gttgtctatg gttcagagct gtaacattgt
    57541 gcgccttcta atcaaaggca taagtccatt tctgtttttt tcatgctggt aagacttcca
    57601 gggagtggtt atctctggcc ctgcaccagg gttaatttgg caattatgca tgctgaacat
    57661 tcttccagca tgtttactgt cacgggcgtt aagacctaaa actgtccccg tttcacctat
    57721 gcgttctctc ccaccatttc cgttatgtgc aacaacttga tttataccta acatttgtga
    57781 acatgctaat agtgcttcaa gtttaggtct gtaatctgtc gaaacagaaa aataacgatt
    57841 aaaggatcga caagagatag cccggctagt tttaaacttt ttgccattaa ttatagcatg
    57901 atgcttttta ttaataattt ccgctaattc gaccggatcg agagcattgg tcatttcatc
    57961 aatagagaca gttatcggtc ctataatgta acgttttcgc tgatcatcaa aagttatccc
    58021 atgatgattt gccattattt tgtatttggg gtcatagaaa catacaggat aatcttttat
    58081 cccgtcgtag gtatcctcat caagtggttt catgcgcgtg aagttttgca tatagttacc
    58141 attgcaattc gtttcatgat ttccagctaa tacatgaaca ttttcattga tactcattat
    58201 gcgttttaac aaatcgatga taaattgatc gccacagata ttactaaatc gatcgccggt
    58261 ttggtcgcca agaaacgtac atggcgtgac tgcgtcttta ttaataacca aataaggctg
    58321 aagactttct aaaacattat ctcttctgtt cagtatactg attattgtat taacattagt
    58381 gtcaagttgc tcattaaagt tctcccttag caatacgttg agaacttcat cttctatatt
    58441 aagcaactgg ctaaggaaat tttctccttc agccatgatt cccaaatgtc ctgttgcaaa
    58501 aagcgcgtac aaaactgcac cgactgagcc atcggtatcg ccaaaatagg tcggcctggt
    58561 acttgtggga tcaacaggtg aacaattgga gaaataggtt ctgtcagcga actggggttt
    58621 tataattgtt tcgaatttaa tatcaaatac attgtattgc gatatttccc agttccagat
    58681 gggtttgtac gactcagtaa ctgcaacatc attagcatta tcatttactg tcatattatt
    58741 aagtaaataa acaatgctgc accaattaat atcggtatta tcaagagggc aaccgtaatt
    58801 tttactatta attgtcacaa taaactgata acagttattc ctacgtagaa tatgagcgcg
    58861 catgcccgat agcatactat atttttctga atgattcgtt tgtatggtaa atgaaatttt
    58921 gtcatcttga atagattgtt ggaaacgttc tatttgccaa tcatcaattt tttccacaac
    58981 ttcaccgagc attgtcctgg caatacttga tatatcatca cctataccat ttgcaatata
    59041 cccaaacagt tctttagtga agttggtaac atgctcttgc accatatcat ttgttgcgat
    59101 tgtcgttgta ctgccattag tgcctgccac agttccaatt ttcatctttt acctcatatt
    59161 attcaccgcg aggaatacat agttaacatt ttatcttatg acatacacct catattcgat
    59221 ggggaaataa ttatattttg cacaaatcat tcagccttaa agcataatat gaaatttctt
    59281 atctcatagc agagaaagtc cctaaatatt gtagaggcgt catcagtaca tacctctaca
    59341 atcgattaag aaaacctatg aaatactggc aaaattaaaa agagatacca cagcactcat
    59401 gaagttttat tgaggttgaa aacaactaag ccctgggaca ccttttttat caccacgttc
    59461 gttaggtgct atcttccaag aatttagccc catggtataa ttatcaattt tttgatgctg
    59521 gtctacccct aatccataat ggccaaattt ctgtttagtt tttttgagcg tctgtccaca
    59581 tttgaagata tcttcaggtt ttggtctgaa agcagtagtg ttattaaaat aattttcata
    59641 tacaggtgtg ggtgctatag cattgcttct aattagatgg agaagatgat catccatgtg
    59701 ttgtttatgt tttttattac atatatttgc taattcctca ggatcaattg ggtctttcat
    59761 ctgattaaat ggaacctgca aagcaccgag acaataacat tgattttctt catttcttat
    59821 tattccgtga tgactggtta aaactttagt ttctgagtta taattacaaa catcgagcgt
    59881 tttaattaag ttatacgtat caccggcaga gagtttataa ttttctagtc gtgcagtata
    59941 gttaccatta aaattgattt catgatttcc agccagaacg atgacatttt tattaatacg
    60001 gctatcttta ttaccctcca tatttcgcat agaatttagc aaagtaagga tatatttatc
    60061 accaaaaata gtactaaatc tatcgcctgt atgatcccca aggaagatac aaggggttat
    60121 cgcatcgtcc tttaaaaaaa catgttgatg caactcattc aatatcatgt ctctcgtatt
    60181 taataaattg tagagcaatt tattattttt atgtatgtat gcagatgaag ccatgtcttc
    60241 gtgttttaat aattcacata ataaactcca acccttttcc ctgatgccta tatgccctga
    60301 aacgagcaat gaataaagta cagcgcctac tcttccatct gtatcgccaa aataagccgt
    60361 gttggcattt tgtgggttaa tcacaccaca ttgtttataa aagtctattt tagaaaacac
    60421 agatttaaca tagctatcaa acttatcctt aggaaacgtc tctttagaga catcccaacg
    60481 ccagtcagct ttaaaattac cgctaagaag agtaaagaaa ttttcaggta tgatagaaag
    60541 cccattaact ttataaatta tgtctgattt atctccggag aaattaataa aataataacc
    60601 atccttacac tcaatcttaa ctgtaatggg agacgataat tttatcacat ggttattgtc
    60661 tggtataatt gtaaagataa acgtgttagt agtattatcg gcaaaactat tatattcttg
    60721 aatatctata gatggctcac tattgtaaaa ccatattttc tccatctgaa gactatgttc
    60781 atttaccctc cagtgaccat gttgcaggaa atagatgata tgcatgccaa aatcgttatc
    60841 tatttgcgaa tcattatcca gcttatcaat ttcaactatc tttgaacgat gtgttttatc
    60901 aaccttgcta tggtgttgat gattattaac tttatctgaa tgcttatctt tatggtttga
    60961 agggatgtac ttaaatatat tgtcaattgc tttcataaat aacccgcgat atttttgacc
    61021 tgttagtaaa aaataaatga tacatgtctg aaagacatat ttacgacgat ataaaacaat
    61081 aatacttata tgttcgctga ttttaagcac cgagactatc cggtctttta ctaaataatg
    61141 gaaaacaatc catccctgga agacaattgt aaaaaaaaac aacagaataa gaattaatct
    61201 taggataaat atttatttat catggccttt tgaccgctaa aaaatggcac tcggcacagt
    61261 aaaccccgct atagttatgc tatgcatttg tctttaatga tatctcgtcg ttatattaac
    61321 aggctgaaat tatcattaat gcacatttca atattagcag ggatacctga ggagtttaaa
    61381 gtgaacatat atatcgggta gcttttcaaa ttaatccctt tgattatagg cttaatttgc
    61441 atcgcgttag gtggctttgt gctggaaagt tcagggcaac gtcacgggtc atgtgctgat
    61501 ttctctggcg gccatatgcc tggcattatt cactaccgca tttattatca tttcgcaact
    61561 cacgcgcggc gttaatacgt tttacaatac attgttcccc attattggct atgcggggtc
    61621 aattatcacc atgatatggg gttgggcact gttagcaggt aatgatgtga tggcagacga
    61681 gtttgttgcc ggccatgtta tttttggcgt tggtatgatt gccgcatgtg tatcgacggt
    61741 ggcagcgtcg tccggtcact ttctgctcat tcccaaaaat gcagcgggga gcaagagcga
    61801 cggaacaccg gtacaggctt attcttcatt aatcggtaat tgcctcattg ccgttcccgt
    61861 tttactcacc ctgctcggtt tcatttggtc tattacgctg ttacatagtg ccgacataac
    61921 tccgcattat gtcgccttgg gttaaccgca atctgtgcct gtctaattgg cctagtggcc
    61981 acaatcgtcc atcaaacacg taatacgttc tcatccaaag agcactggct gtggtgttat
    62041 tgggttattt ttctcggctc agtcacggtg ctgcagggga tatacgtctt agtcagttcc
    62101 gatgcaagcg cccgactggc tcccggcatt attctcattt gcctcggaat gatctgttac
    62161 agcatattct caaaagtctg gctactggca ctggtctgga gacgtacctg ttcgttagcc
    62221 aacagaatac cgatgatacc ggtatttacc tgtctgtttt gcttgttttt gtcatcattc
    62281 ctcgcggaaa tcgcacaaac tgagctggac tactttattc catctcgggt tctggttgga
    62341 ttaggcgcag tatgttttac attgttctct attgtttcaa tattagaggc cggttctgcg
    62401 aaaaaaaatt aaaatttaca taatcccagc gttattcata tcttaacgct ggttttttac
    62461 gaacttagtg aaataatgta gaatataaaa ttataatttt atattggagc acatgtgtta
    62521 atgatcaacc ccgggatact aaaaatatag acattattaa acttgcttag cctcgcattg
    62581 cctttacctg ttaaagatgc atcgagaaaa ttgagtctga agttctcatc ctttatctga
    62641 ttttccagtc ccgaaacttt ggccttagca ttacctgaga tccgtccgtt actatctatt
    62701 ttgtaggtaa aaaaatacga atgggaaaat ttcctcggtg tatcttcatc agttaagcca
    62761 acaatcgtaa aactaccgct accatctttt tttaaaatga ctgaaaggtt agcagagtag
    62821 tataaagaat gatctgtttt agcatttctt gttaacgtcg acgtacattc aagagacgtt
    62881 gtgctaccat cacgagcctt ccagagagca aaaaaacaca cctgtagcaa tgtgaaaaca
    62941 agtattaata taaaatacat tttcttatta gtcatataat agcacctctg aacaaattgc
    63001 ataagtttcg tcattagagg attttataca tcttgataaa taccgctgcc cttccccaag
    63061 caaataatgc atactattta catcatatat aaaatttgca cctggcacac agggtaaatg
    63121 ctttttcgaa atttcagcgg cctctatttg agatttagcg aggccaatct cgttaagatt
    63181 ctctgaaaga tataaatcac aggtgtcaat cttacctgat ttaaccaatg gataatccgt
    63241 tatactgtag gtatctttaa aagccacggc aaacatttca ataataacaa ttactaccac
    63301 aagcgaaatc aaaatgcgaa aaagaggaat actccttttg cgctctactt tttcaacaac
    63361 atcttcttgt aaatgagaat catcataaga ttgtttactt attgttatgt tttcactgat
    63421 gtaaaagcct actttgggta tcgtaagaat cacattttct tcaacaccaa tatttttaaa
    63481 acttttgcgt aacagggata tatattggtt caatgtgttg tttgacagat caaatgaaaa
    63541 tctctccaaa acgctatcaa tgattgagtt cctgtcaacc gctccaccat tactttccag
    63601 taacactgcc aaaatacacc ccgccgtagc tggaaaattt atctcatcct gggtatttac
    63661 attcgagagc gagcatttgt tcggatcaaa aataatcgcg tcattgataa tgaaaaaaca
    63721 tctccgctcc atgcaggcaa caacacaaaa taagattttg tcattttagc ctattcctgg
    63781 tggaactgac acaagaaact aaaatacaaa tataaccctg aaaaagtcat ataaaactta
    63841 tatccacaga ataaaaaatc aaacatgtca gttttatcaa tataaagtat cgcaaggcga
    63901 aaagtgatgc aagaatactc ctaagtaaat gaaagcgcac cacatgaaat tatcaacaca
    63961 tgtaatttca tcattaggtg ctatcgaaaa taactcagga gctacttaag atgaataatg
    64021 tcttactcat gagacccgat gactcagaat ttatgatacc tgatgactgg tcagggctaa
    64081 acgcaaaagg cattacatac gaacaagata atagagtgac agagtctttt ctgcctttta
    64141 aatcttttta ttgttcaccg gtgtgcacga cagttgaact catctcacaa aataattgtt
    64201 ttgctatggg tatccgctac cttctggaaa agaaaagaga cttaaggcta aatcacttta
    64261 aagccttctc tcataaatgc gagacaaaac ctgacgtgtt aattatgacc gggtgcaccg
    64321 tcgctgatac ttatagtata ttctcacgca ttgttgaaga aaatataatg aaaacgatga
    64381 cacctatcgt catcattact gggcatcact cagaagatct ttgttctctg gcatcgaaac
    64441 tgggaggagt taaaataata tcaccgacag aaaagattgc aaaaatagaa aacgctatca
    64501 atgatgcttt taacagcaag cctaaaaaaa taagattttt aacagatata ctctctcctc
    64561 aacaaattga gaccatgaaa atggttgcgc agggtagatc cgttaatgag atttcagccg
    64621 caatgaatat tagcatcaag accgtgtatg cacataaagt tcgtgctatc aataagctgg
    64681 gaattaagaa aaaagtttat gaagcgcttt tttataaagc gctaattcag ttcaactgat
    64741 gtaaaaatca ccctcgctta ataaataata tattctatgt ctttttttat aagttaaaaa
    64801 aggcgggata tcaaatcccg ccatgaaatt aactaatcat ctgcattatt ctgcgtagat
    64861 ataagcctgg gtacggatgg tgtaagagtc tggctcaaca acctgttctt tcatatggga
    64921 aacgacgcga taggcaccac ctgttccagt atttttcagc atcatataac gcttaccgcc
    64981 aatctcttcg gaggcagctg aaggaggatc ctgctcaagc gttgtcaggg aaactttcag
    65041 tttatggtcc gggttattgt caccagaaat tactgcatcc gctgaagtac tgctctccgc
    65101 ataaatatca tccgggaaag ttacagcata gtcgtggctc tgcccatcag atgcgccaac
    65161 aatacctgtt gccaaaggcg tccatgcagt taacgtaccg ctatataccc ctttcactgg
    65221 ttgaatggta ttattaacaa cgataggctt ggtcaaagta agattagctg acgcttcagt
    65281 accctggctt actaaagttc ccgcctgaga agcaaaagct gtccccgcca gcatgattgc
    65341 taacaatgtt ttcttcatta tattttctcc atgattttca tttacactgc aaaatgcagt
    65401 ttctttcttt gcatttacgt gctatcgcga aaatgcttcc accaaataat ggaaacatct
    65461 tcacaaaaat aaattatgag tttcttaatt tagaaactat aaacatgtcg ccgctatctg
    65521 gtataaccct gatgcacttt tcttcgcagg aagttggtat tttgcgatac aggattgatc
    65581 tttagaataa ccccatttca cattaaactc gccttccgca ctcaagccag aaacataagc
    65641 aataccatct tcacctacaa tgccggccat attttcagtg tctttgctat aactaatcac
    65701 agcgccgaag gggatgggtt taccctcttt ctgttttaag gttactaatc ctttatcccc
    65761 gctcttcact gccaatgtcg caggcacaac ggctccagct gtcggtacaa ccaattgact
    65821 agtttgtggt aagtcgtaat tcttacctat cgttttttca tccagaataa catcgtctct
    65881 tcggtatggc tgcactggca gtattgcata cccacgaaaa tcagtactca gtcccggcca
    65941 gccattaacg ttaacccccg ttgctccggg tgcctctata agggctaatg tcccatccac
    66001 attttgacca aatgtaagtc catcccttgt aataagtatc ccaccgctta aatttgcacc
    66061 tagctgtttc atgttttcgc tggagctgta atggctacta aactgaccat atgatccatt
    66121 tttgccaact tgcacaatgt tcatcgtgct cttcggttgt gactgatgag cgttagttat
    66181 atttgtcgct acatcccacc atacaccacc actattctga cctgataatg aaacctgata
    66241 gttattaccc tgctctcccg aacgactcat ctgtgaagac gcataaattt gattttcgtc
    66301 acccagcgat aatggcactg aaatacccac gcttaatttc cgatctactt tccatttatg
    66361 cgatgtcagc aagtcgtttt ttgcccagtt aatggagaat gttatgcctc tccataccgt
    66421 actataactc aggctggtag accgcgtgtt tcctgtgccc cgggaagaat tatacctcgt
    66481 ggtattgaag ttcaaattcc cgatattacc aaggggctgg gataaggata tcgtagagcc
    66541 atcgtgacta ttcttatctc tttttctgtt ccgttcatcc tgtgcactta ctgcttcagt
    66601 cagtgtggca taacctgatg cataacggtt cgtggttata ga
//
LOCUS       DAJTSA010000010        65606 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000010 DAJTSA010000000
VERSION     DAJTSA010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.010"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(128..607)
                     /gene="folK"
                     /locus_tag="OO369_000683"
     CDS             complement(128..607)
                     /gene="folK"
                     /locus_tag="OO369_000683"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR5981067.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="OO369_000684"
     CDS             complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="OO369_000684"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR5981068.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="OO369_000685"
     CDS             complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="OO369_000685"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR5981069.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="OO369_000686"
     CDS             complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="OO369_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR5981070.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="OO369_000687"
     CDS             complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="OO369_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR5981071.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="OO369_000688"
     CDS             complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="OO369_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR5981072.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4980..7409
                     /gene="hrpB"
                     /locus_tag="OO369_000689"
     CDS             4980..7409
                     /gene="hrpB"
                     /locus_tag="OO369_000689"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR5981073.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7605..10139
                     /gene="mrcB"
                     /locus_tag="OO369_000690"
     CDS             7605..10139
                     /gene="mrcB"
                     /locus_tag="OO369_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR5981074.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10359..12602
                     /gene="fhuA"
                     /locus_tag="OO369_000691"
     CDS             10359..12602
                     /gene="fhuA"
                     /locus_tag="OO369_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR5981075.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12653..13450
                     /gene="fhuC"
                     /locus_tag="OO369_000692"
     CDS             12653..13450
                     /gene="fhuC"
                     /locus_tag="OO369_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR5981076.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13450..14340
                     /gene="fhuD"
                     /locus_tag="OO369_000693"
     CDS             13450..14340
                     /gene="fhuD"
                     /locus_tag="OO369_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR5981077.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14337..16319
                     /gene="fhuB"
                     /locus_tag="OO369_000694"
     CDS             14337..16319
                     /gene="fhuB"
                     /locus_tag="OO369_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR5981078.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGCVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="OO369_000695"
     CDS             complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="OO369_000695"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR5981079.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17859..19280
                     /gene="clcA"
                     /locus_tag="OO369_000696"
     CDS             17859..19280
                     /gene="clcA"
                     /locus_tag="OO369_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR5981080.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19304..19369
                     /locus_tag="OO369_000697"
     CDS             19304..19369
                     /locus_tag="OO369_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981081.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19362..19706
                     /gene="erpA"
                     /locus_tag="OO369_000698"
     CDS             19362..19706
                     /gene="erpA"
                     /locus_tag="OO369_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR5981082.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19753..20376)
                     /locus_tag="OO369_000699"
     CDS             complement(19753..20376)
                     /locus_tag="OO369_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR5981083.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="OO369_000700"
     CDS             complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="OO369_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR5981084.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="OO369_000701"
     CDS             complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="OO369_000701"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR5981085.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21989..23506
                     /gene="dgt"
                     /locus_tag="OO369_000702"
     CDS             21989..23506
                     /gene="dgt"
                     /locus_tag="OO369_000702"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR5981086.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23636..25060
                     /gene="degP"
                     /locus_tag="OO369_000703"
     CDS             23636..25060
                     /gene="degP"
                     /locus_tag="OO369_000703"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR5981087.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25215..26371
                     /gene="cdaR"
                     /locus_tag="OO369_000704"
                     /pseudo
     CDS             25215..26371
                     /gene="cdaR"
                     /locus_tag="OO369_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26460..26846)
                     /locus_tag="OO369_000705"
     CDS             complement(26460..26846)
                     /locus_tag="OO369_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR5981088.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="OO369_000706"
     CDS             complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="OO369_000706"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR5981089.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="OO369_000707"
     CDS             complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="OO369_000707"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR5981090.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30749..31543)
                     /gene="map"
                     /locus_tag="OO369_000708"
     CDS             complement(30749..31543)
                     /gene="map"
                     /locus_tag="OO369_000708"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR5981091.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31911..32636
                     /gene="rpsB"
                     /locus_tag="OO369_000709"
     CDS             31911..32636
                     /gene="rpsB"
                     /locus_tag="OO369_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR5981092.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32894..33745
                     /gene="tsf"
                     /locus_tag="OO369_000710"
     CDS             32894..33745
                     /gene="tsf"
                     /locus_tag="OO369_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR5981093.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33892..34617
                     /gene="pyrH"
                     /locus_tag="OO369_000711"
     CDS             33892..34617
                     /gene="pyrH"
                     /locus_tag="OO369_000711"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR5981094.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34909..35466
                     /gene="frr"
                     /locus_tag="OO369_000712"
     CDS             34909..35466
                     /gene="frr"
                     /locus_tag="OO369_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR5981095.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35558..36754
                     /gene="ispC"
                     /locus_tag="OO369_000713"
     CDS             35558..36754
                     /gene="ispC"
                     /locus_tag="OO369_000713"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR5981096.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36943..37701
                     /gene="ispU"
                     /locus_tag="OO369_000714"
                     /gene_synonym="uppS"
     CDS             36943..37701
                     /gene="ispU"
                     /locus_tag="OO369_000714"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR5981097.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37714..38571
                     /gene="cdsA"
                     /locus_tag="OO369_000715"
     CDS             37714..38571
                     /gene="cdsA"
                     /locus_tag="OO369_000715"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5981098.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38583..39935
                     /gene="rseP"
                     /locus_tag="OO369_000716"
     CDS             38583..39935
                     /gene="rseP"
                     /locus_tag="OO369_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR5981099.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39965..42397
                     /gene="bamA"
                     /locus_tag="OO369_000717"
     CDS             39965..42397
                     /gene="bamA"
                     /locus_tag="OO369_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR5981100.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42519..43004
                     /gene="skp"
                     /locus_tag="OO369_000718"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42519..43004
                     /gene="skp"
                     /locus_tag="OO369_000718"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR5981101.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43008..44033
                     /gene="lpxD"
                     /locus_tag="OO369_000719"
     CDS             43008..44033
                     /gene="lpxD"
                     /locus_tag="OO369_000719"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR5981102.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44138..44593
                     /gene="fabZ"
                     /locus_tag="OO369_000720"
     CDS             44138..44593
                     /gene="fabZ"
                     /locus_tag="OO369_000720"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR5981103.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44597..45385
                     /gene="lpxA"
                     /locus_tag="OO369_000721"
     CDS             44597..45385
                     /gene="lpxA"
                     /locus_tag="OO369_000721"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR5981104.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45385..46533
                     /gene="lpxB"
                     /locus_tag="OO369_000722"
     CDS             45385..46533
                     /gene="lpxB"
                     /locus_tag="OO369_000722"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR5981105.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46530..47126
                     /gene="rnhB"
                     /locus_tag="OO369_000723"
     CDS             46530..47126
                     /gene="rnhB"
                     /locus_tag="OO369_000723"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR5981106.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47163..50645
                     /gene="dnaE"
                     /locus_tag="OO369_000724"
     CDS             47163..50645
                     /gene="dnaE"
                     /locus_tag="OO369_000724"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR5981107.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50658..51617
                     /gene="accA"
                     /locus_tag="OO369_000725"
     CDS             50658..51617
                     /gene="accA"
                     /locus_tag="OO369_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR5981108.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51716..53857
                     /gene="ldcC"
                     /locus_tag="OO369_000726"
     CDS             51716..53857
                     /gene="ldcC"
                     /locus_tag="OO369_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR5981109.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53914..54303
                     /locus_tag="OO369_000727"
     CDS             53914..54303
                     /locus_tag="OO369_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5981110.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54368..55666
                     /gene="tilS"
                     /locus_tag="OO369_000728"
     CDS             54368..55666
                     /gene="tilS"
                     /locus_tag="OO369_000728"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR5981111.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55715..55975)
                     /gene="rof"
                     /locus_tag="OO369_000729"
     CDS             complement(55715..55975)
                     /gene="rof"
                     /locus_tag="OO369_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR5981112.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55962..56162)
                     /locus_tag="OO369_000730"
     CDS             complement(55962..56162)
                     /locus_tag="OO369_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR5981113.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56328..56873
                     /locus_tag="OO369_000731"
     CDS             56328..56873
                     /locus_tag="OO369_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR5981114.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56870..57292
                     /gene="arfB"
                     /locus_tag="OO369_000732"
     CDS             56870..57292
                     /gene="arfB"
                     /locus_tag="OO369_000732"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5981115.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57306..58016
                     /gene="nlpE"
                     /locus_tag="OO369_000733"
                     /gene_synonym="cutF"
     CDS             57306..58016
                     /gene="nlpE"
                     /locus_tag="OO369_000733"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR5981116.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58171..58995)
                     /locus_tag="OO369_000734"
     CDS             complement(58171..58995)
                     /locus_tag="OO369_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR5981117.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59048..60766)
                     /gene="proS"
                     /locus_tag="OO369_000735"
     CDS             complement(59048..60766)
                     /gene="proS"
                     /locus_tag="OO369_000735"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR5981118.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="OO369_000736"
     CDS             complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="OO369_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR5981119.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="OO369_000737"
     CDS             complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="OO369_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR5981120.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="OO369_000738"
     CDS             complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="OO369_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR5981121.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62958..63611)
                     /gene="metI"
                     /locus_tag="OO369_000739"
     CDS             complement(62958..63611)
                     /gene="metI"
                     /locus_tag="OO369_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR5981122.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63604..64635)
                     /gene="metN"
                     /locus_tag="OO369_000740"
     CDS             complement(63604..64635)
                     /gene="metN"
                     /locus_tag="OO369_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR5981123.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64823..65398
                     /gene="gmhB"
                     /locus_tag="OO369_000741"
     CDS             64823..65398
                     /gene="gmhB"
                     /locus_tag="OO369_000741"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR5981124.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15508 a        16258 c        18079 g        15761 t
ORIGIN      
        1 tgggattgat atttatctgc gggaatttat attatgtttc tgcaaaaatg tttaaactaa
       61 aatttttatt aaggaaagag aggatgtatg attttcagaa aatatttata ttcgtaatat
      121 aaataaatta ccatttgctt aatttgtcaa atgctcttgt atgtaagact tcacgcaacg
      181 tctccccatc aggaaacgcc agctccggtg caatctcaaa cagcggccac agcataaatc
      241 cacgattctt catatcgtag tgcggaacgg tcaggcgttc ggtatttatc acttcattac
      301 caaacagcat gatatcgaga tccagcgtgc gtggtcccca gcgttcagct ttacgaacac
      361 gaccctgttg cagttctatg cgctgtgtat ggttgagtaa ctcttccggg gcgggcgaag
      421 tttcaagcgc cacggcggcg ttgaggtaat cgggttgatc ttgcggcccc agcggtgggg
      481 tgcggtaaaa cgaagaaacg gcaagaatgc ggctttcagg gatatcgcct aatgctttca
      541 gggcagcatt gacctgctcc agcggagagg ccagattgct gcctatggca atatacgcca
      601 ctgtcatgtg gtaccctcac gacgcggtgc gcgtttgcgt ggacgacgag tacgacgacg
      661 cggtgacggt tcttcatcca gctcgttaag catccctttt tgatctggtg gtgcggaaac
      721 ctggaactca ccccaccatt tcaccagacg ctgcagttca gcattacgtt caacttcagc
      781 tcgcaaggcc aacaggtcat aagccgcacg gaacttagga tgctccagca gtttccatgc
      841 gcgtttaccc tgacgacggg acatacgcaa ctgcaactgc cagatatcgc gggttaacgt
      901 cgtcagacgt ttcgggattg ccagtgaacg gcaggcttcg tccagcacgt cgttcatcgc
      961 cagcgcgaaa gcgtcgtgat aggtcaggcc gctttcctgg gcgatcttct gtgccgtctc
     1021 cagcagtggg taccagaaca tggcggcaaa caagaacgcc gggttcacgc gcatatcgtt
     1081 atggatacgc gtatcggtat tcttcagcac ctgttcaatg atccgctcca tcgggctgtc
     1141 gccattttcc gtgaagtagc gggtaatggt cgggaacagc ggctggaaca gatgatattc
     1201 acacaacagc ttataggttt cgtaaccgta gcccgcttgt agcagtttaa gcgattcttc
     1261 aaacaggcgt gccggtggga tatcgttcag cagggtagcg aggcgaggga tcggttctgc
     1321 ggtttccggg ctgatgcgca tacccaattt ggcggcaaaa cgtaccgcgc gcagcatacg
     1381 taccggatct tcacggtagc gcgtttccgg gttaccaatc agacggataa cgccgtcctt
     1441 cagatccttc ataccaccaa cgtaatcacg gacggtaaaa tccgctacgc tgtaatacag
     1501 gctgttgata gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga aaatgttgtc
     1561 gcgcagcaac atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga cgttaccttc
     1621 gtggtgtcca cggaaggtcg caacttcgat aatctccggg ccaaacatta catgagccag
     1681 acggaaacgg cgacccacca ggcggcagtt acggaacagt ttgcgcacct gctcaggcgt
     1741 ggcgttagtg gttacgtcaa aatctttcgg ctttttgcca agtaacaggt cgcgcacgcc
     1801 gccgccaacc agccaggctt cgtatcccgc tttgttgagc ctgtacatta ccttcagggc
     1861 attttcactg atatctttgc gggaaatagc atgctgctca cgcgggatca ccgtcacctg
     1921 tggacgggcg actgcctgtt cagcctcgct ttcctcgcgg cttagcacct tgcggcaaaa
     1981 attagcgact cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt caggacaaaa
     2041 aaatagcggc taatcatagc tcagcatgac gcatttgaga atgttgaatt tacaattgcc
     2101 gactcgggca cggcggtcag catccagttt ttgacggctg actgaaggat ttgctcgacg
     2161 ctgaaatcct gccagtgtgc ttctgcctgc tgccccagaa attgaagtgc cgcgattagt
     2221 accgggcgtg gatcgccttt cggcaacgca ggcgcatgat tctgcttgga aagtttagcg
     2281 ccttgtggat taagcgccag cggcagatga atgtaatctg gcactttcca gccaaaaagc
     2341 tggtacagcg ggatttgcct tactgttggt tcaatcagat cagccccacg cactatttct
     2401 gtaacgccct ggaaatgatc atcaaccaca acagccaggt tgtaggcgaa caacccatca
     2461 cggcgatgaa tgataaaatc ttcccgtgcc agtttttcgt cggcgtgaat aatgcctcgc
     2521 agcaggtcag taaattgcgt gaccggatgc tgctggcgga tacgcactgc ggcgttgtct
     2581 ggtccatgat gcaacacccg gcaatgaccg tcgtaaatac cgccaatgct ttgaatacgc
     2641 gcacgcgtac aggtgcagta ataacttagt ccttgttcat gtaaccaggc gagtgcttca
     2701 cgataggcgt cgtgacgttg cgattgccag agaacatcgc cgtcccagtg cagaccgtaa
     2761 tgttccagct ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg cgggtcgata
     2821 tcttctatgc gtaccagcca gcgaccttgc cgggcgcgag cctgcaaata gctgccgagc
     2881 gcggcgatca gagagccaaa atgaagctcg ccggaaggag agggggcgaa gcggccaata
     2941 tactgtgtgt ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc ccgcctgtgg
     3001 taaacgtgat ggaacggctg taattagcca gccatctgtt tttcgcgaat ttcagccagc
     3061 gttttgcagt cgatgcacag atcggctgtc gggcgcgctt ccagacggcg aataccaatt
     3121 tcaacaccgc aggattcgca gtagccgaaa tcttcgtctt ccactttttt cagcgtcttc
     3181 tcgatctttt tgatcagctt acgctcgcga tcgcggttac gcagttcgag gctgaactct
     3241 tcttcctggg ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc ctgcatatgt
     3301 gtaacggtgc gatcgacttc atccctgagt tgattacgcc atgcttccag aatacgacgg
     3361 aagtgcgcca gctgggcttc attcatatac tcttcgcccg gcttctcctg atatggttcc
     3421 accccagcga tggcgagaat actcagggac gatgttttac ggttttgccc ttcttgcatg
     3481 ttgcttctcc ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg aggccgctat
     3541 aaatagcaga tgcttttccg gatagcaatt atctaaacgt aacacttgac aactgtgtga
     3601 ggaaaagcgt atttgcgcac gcgaccagaa tgtaaattaa ccagttactt actttactac
     3661 aatgtaaccg gcagtgattt tttaagagcc atgccttcag cagaaatttc cgctttgtaa
     3721 gccagaattt ctacccccct ctgttgagct tctgacaata gttgcgcgta tttctcatcg
     3781 atgtggcgcg cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa aaaaataacc
     3841 gcacgctggc cttcagccgc tacgctcatc aactcccgaa ggtgtttctg acctcgttca
     3901 gtgaccgcat cgggaaaata tccctgttcg ttctccgcta acgtaaccga tttcacttca
     3961 atatagcagt ctggacgcga atccgcctgc aacataaagt caatacggct gcgttcggag
     4021 ccgtatttta cttcgctttt cagcgagcta tagcctgaca gttctgaaat tgattcatta
     4081 aggatagcct ctttcgtcaa cctgttagcc caaagcgtat tgacgcaaat aaatgcgccg
     4141 ctctggcttt gagttaattc ccaggtgtgt gggtatttcc gtttggtgtt gtctgaagtc
     4201 gaataccaga cggtatcgcc aggcgttgca caaccggtca tcgcacccgt attcgggcag
     4261 tgtagcgtta attcgcgacc atcgggtgtg atcacatcgg ctaaaaaacg tttgtaacgc
     4321 tgaattagcg tcgcgcgctg tagaggggga gaaaattcca ttgcgacaat ccttgttatt
     4381 gcgttagcgc ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca aacgaggagg
     4441 cgtaaagggt gaactccgtc accgcataag accagttaaa acccggcggc gggaatgtca
     4501 ccgcctcgct ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga ttgctttgaa
     4561 aacaaccgct gcgggcagcc tgtgaacgga gcatattcgc cagctggatt aagccgcgtg
     4621 gaggctgacg catccctaac cacaccacac gcgaacgcag ccattgtccg gcgtcatcaa
     4681 gcgtgagtgt gaaaccaggt tgacgaatcc gtccggctaa aagagaaagc gccttctctt
     4741 tctctgcgct cacttcgcct aaaaatgcca gagtcaggtg caaattatcg gcggcgaccg
     4801 gacgtcccgc ctcaggtggg aagtgtgtgg cgcgccaatg gataatctgt tcgcggattt
     4861 ctgcaggtaa gtcgatagca aagaacagac gttgcggttc agacatgtga ggcactcggt
     4921 tatgaattac cgcgatgcta caatgtggcg cgaagaatgt taaccctctg gagcgttttg
     4981 tgtcgtcgtt gcccgttgct gccgtcttac ctgaattact taccgctctc gattgtgcgc
     5041 cgcaggtatt attaagtgcg ccgaccgggg ccgggaaatc aacctggctg ccgctgcaac
     5101 tgctggcgca tcccggcatt aacgggaaaa ttatcctgct ggagccgcgt cgtctggcgg
     5161 cgcgtaacgt cgcgcaacgg ctggcggagc tgcttaacga aaagccaggc gataccgttg
     5221 gctaccggat gcgtgcgcaa aactgcgtcg ggctgaatac ccgcctggaa gtggttaccg
     5281 aaggcgtgct gacgcggatg atccagcgtg acccggaact gagcggtgtt ggattggtga
     5341 tccttgatga gtttcatgag cgcagtttgc aggcggattt ggcgttggcg ctgttactcg
     5401 atgtgcaaca aggtctgcgt gatgacctta aactgctgat tatgtcggct acgctggaca
     5461 atgaccgctt gcagcaaatg ctgccggaag cgcccgtcgt catctcagaa gggcgctcgt
     5521 ttccggttga acgccgttat ttaccgctgc ccgcgcatca gcgttttgac gaagccgttg
     5581 cggtagccac cgccgaaatg gtgcgtcagg aaagtggatc attactgtta tttttacctg
     5641 gcgtcggaga aattcagcgc gtgcaggaac aactggcttc gcgcatcggc agtgatgtgg
     5701 tgctctgccc gctgtatggc gtgttgtcac tgaacgatca gcgaaaagcg atcctcccgg
     5761 caccgcaagg gatgcgcaaa gtggtgctgg cgaccaatat tgctgaaacc agtttaacca
     5821 tcgaaggtat tcgtctggtg gtggattgtg cccaggagcg tgtggcgcgc tttgatccgc
     5881 gcacggggct tacgcggttg attactcaac gcgttagtca ggcatccatg acgcagcgtg
     5941 ccgggcgcgc cgggcgtctg gagccgggta tcagcctgca tttaatcgcc aaagaacaag
     6001 cagaacgcgc cgcggcgcaa agtgagccgg agatcttaca aagcgatctt tccggtttgc
     6061 tgatggaatt actgcaatgg ggatgcagcg atccggcgca gatgagctgg ctggatcaac
     6121 cgccaacggt gaatctactg gccgcgaaac gcctgttaca aatgctgggg gcgctggagg
     6181 gtgaacggct tagtgcgcaa gggcaaaaaa tggcagcgct gggtaacgat ccgcgtttag
     6241 cggcaatgct ggttaacgca aagagcgatg acgaagctgc taccgcggca aaaattgccg
     6301 ccattctcga agagccgcca cggatgggca atagtgacct gggcgtggcg ttttcgcgca
     6361 atcaaccagc ctggcagcaa cgtagtcagc aactgttaaa acgcttaaac gtacgcggcg
     6421 gtgaggcaga cagttcgctt atcgcgccgc tacttgccgg ggcgtttgcc gatcgcattg
     6481 ctcatcgccg tgggcaagat ggacgctatc aactggcaaa cagcatggga gcgatgctcg
     6541 atgccgacga cgcgctaagc cgccacgaat ggttgatcgc accgttatta ttgcagggca
     6601 gcgcctcgcc ggatgcgcgg attttactgg cgctaccggt cgatattgat gaattagtac
     6661 aacgctgtcc gcagctggtg cagcaatctg acaccgtgga atgggatgac gcgcaaggta
     6721 cgctgaaagc ctggcgtcgg ctacaaatcg gtcagttgac ggtgaaagtg cagccgctgg
     6781 cgaaaccgtc agaagacgag ttgcatcagg cgatgcttaa tggcatccgt gataaaggtt
     6841 taagcgtgct caactggacg gcggaagcgg aacagctacg cttgcgtttg ttatgcgccg
     6901 caaagtggtt gccggaatat gactggccag cggttgatga tgaaagtttg ttggcgacgc
     6961 tggaaacgtg gctgctgcca catatgaccg gcgtacattc actacgcggc ctgaaatcac
     7021 tcgacattta tcaggcacta cgcggattac ttgattgggg aatgcagcaa cgtctggata
     7081 gtgaattgcc tgcgcattac actgtgccga cgggaagccg gatcgccatt cgttatcatg
     7141 aagataaccc gcccgcgctg gcggtgagaa tgcaggagat gtttggtgag gccactaatc
     7201 cgacgatcgc ccaggggcgc gtgccgctgg tgctgaagtt gctttcccct gcccaaaggc
     7261 cgctgcaaat cacgcgtgat ttgggggcct tctggaaagg ggcgtatcgt gaggtgcaaa
     7321 aggagatgaa agggcgttat cccaaacacg tctggccgga cgacccggca aacacggcac
     7381 cgacgcgacg gacgaaaaag tattcgtaag gttggttttc tccctctccc tgtgggagag
     7441 ggccggggtg agggcatcag cgcgcacgtt caccctcacc ctaaccctct ccctcaaggg
     7501 agaggggacc gatcgagcgc aaattttgag agatatcttc ttctgtcttg taacagaaga
     7561 acagaaaatc gggcttttgc gcctgaatat tgcggagaaa aagcatggcc gggaatgacc
     7621 gcgagccaat tggacgcaaa gggaaaccga cgcgtccggt caaacaaaag gtaagccgtc
     7681 gtcgttacga agatgacgat gattacgacg attatgatga ctatgaggat gaagaaccga
     7741 tgccgcgcaa aggtaagggc aaaggcaaag ggcgtaagcc tcgtggcaaa cgcggctggc
     7801 tatggctact gctaaaactg gctatcgttt ttgccgtgct gatcgccatt tacggcgttt
     7861 atctcgatca aaaaattcgt agccgtattg atggcaaggt ctggcaactg cccgcggcag
     7921 tttatggccg aatggtcaac cttgaaccag atatgaccat cagcaagaac gaaatggtga
     7981 agctgctgga ggcaacacag tatcgtcagg tgtcgaaaat gacccgtcct ggcgaattta
     8041 ccgtgcaggc caacagcatt gagatgatcc gccgtccgtt tgatttcccg gacagtaaag
     8101 aagggcaggt gcgtgcgcgt ctgacctttg atggtgatca tctggcgacc atcgtcaata
     8161 tggagaacaa ccgtcagttc ggtttcttcc gtcttgatcc gcgtctgatc accatgatct
     8221 cctcgccaaa cggtgagcag cgtttgtttg tgccgcgcag tggtttccct gatttgctgg
     8281 tggatacttt gctggcgaca gaagaccgtc atttttacga gcatgatgga atcagtctct
     8341 actccattgg gcgtgcggtg ctggcaaacc tgaccgcagg tcgcacggtg cagggtgcga
     8401 gtacgctgac gcaacagctg gtgaaaaacc tgttcctctc cagcgagcgt tcttactggc
     8461 gtaaagcgaa cgaagcttac atggcgctga tcatggacgc gcgttacagc aaagaccgta
     8521 ttcttgagct gtatatgaac gaggtgtatc tcggtcagag cggcgacaac gaaatccgcg
     8581 gcttcccgct ggcaagcttg tattactttg gtcgcccggt agaagagcta agcctcgacc
     8641 agcaggcgct gttagtcggt atggtgaaag gggcgtccat ctacaacccg tggcgtaacc
     8701 cgaaactggc gctggagcga cgtaatctgg tgctgcgtct gctgcaacag caacagatta
     8761 ttgatcaaga actctatgac atgttgagtg cccgtccgct gggggttcag ccgcgtggtg
     8821 gggtgatctc tcctcagcca gcctttatgc agctggtgcg tcaggagttg caggcaaaac
     8881 tgggcgataa ggtaaaagat ctctccggcg tgaagatctt cactaccttt gactcggtgg
     8941 cccaggacgc ggcagaaaaa gccgccgtgg aaggcattcc ggcactgaag aaacagcgta
     9001 agttgagcga tcttgaaact gcgattgtgg tcgtcgaccg ctttagtggt gaagttcgtg
     9061 cgatggtcgg aggttctgag ccgcagtttg cgggctacaa ccgtgcgatg caggcgcgtc
     9121 gttcgattgg ttcccttgca aaaccagcga cttatctgac ggctttaagc cagccgaaaa
     9181 tctatcgcct gaacacgtgg attgccgatg cgccaatcgc gctgcgtcag ccgaatggcc
     9241 aggtctggtc accgcagaac gatgaccgtc gttatagcga aagcggcaga gtgatgctgg
     9301 tggatgcgtt gacacgttcg atgaacgtgc cgacggtaaa tctggggatg gcgctggggc
     9361 tgcctgcggt tacggagacc tggatcaaac tgggcgtacc gaaagatcag ttgcatccgg
     9421 ttccggcaat gctgctgggg gcgttgaact taacgccaat cgaagtggcg caggcattcc
     9481 agaccatcgc cagcggtggt aaccgtgcac cgctttctgc actgcgttcg gtaatcgcgg
     9541 aagatggtaa ggtgctgtat cagagcttcc cgcaggcaga acgcgctgtt ccggcgcagg
     9601 cggcgtatct gacactatgg accatgcagc aggtggtaca acgtggtacg ggtcgtcagc
     9661 ttggggcgaa atacccgaac ctgcatctgg cagggaaaac agggactacc aacaataacg
     9721 tagatacctg gtttgcaggc attgacggca gcacggtgac catcacctgg gtaggccgtg
     9781 ataacaacca gccgaccaaa ctgtatggag ccagcggggc aatgtcgatt tatcagcgtt
     9841 atctggctaa ccagacgcca acgccgctga atcttgttcc gccagaagat attgcagata
     9901 tgggcgtgga ctacgacggc aactttgttt gcagcggtgg catgcgtgtc ttgccggtct
     9961 ggaccagcga tccgcaatcg ctgtgccagc agagcgagat gcagcagcag ccgtcaggca
    10021 atccgtttga tcagtcttct cagccgcagc aacagccgca acagcaacct gctcagcaag
    10081 agcagaaaga cagcgacggt gtagccggtt ggatcaagga tatgtttggt agtaattaac
    10141 atctaagcgt gaaataccgg atggcgagtt gccatccggt aaaataacat cccatctaag
    10201 atattaaccc tttcttttca tctggttgtt tattaaccct tcaggaacgc tcagattgcg
    10261 taccgcttgc gaacccgcca gcgtttcgaa tattatctta tctttataat aatcattctc
    10321 gtttacgtta ttattcactt tacatcagag atataccaat ggcgcgttcc aaaactgctc
    10381 agccaaaaca ctcactgcgt aaaatcgcag ttgtagtagc cacagcggtt agcggcatgt
    10441 ctgtttatgc acaggcagcg gttgaaccga aagaagacac tatcaccgtt accgctgcac
    10501 ctgcgccgca agaaagcgca tgggggcctg ctgcaactat tgcggcgcgg cagtcagcta
    10561 ccggcactaa aaccgatacg ccgattcaaa aagtgccaca gtctatttct gttgtgaccg
    10621 ccgaagagat ggcgctgcat cagccgaagt cggtaaaaga agcgcttagc tacacgccgg
    10681 gtgtctctgt tggtacgcgt ggcgcatcca acacctatga ccacctgatc attcgcggtt
    10741 ttgcggcaga aggccaaagc caaaataact atctgaatgg cctgaagttg cagggcaact
    10801 tctataacga tgcggtcatt gacccgtata tgctggaacg cgctgaaatt atgcgtggcc
    10861 cggtttccgt gctttacggt aaaagcagtc ctggcggtct gttgaatatg gtcagcaagc
    10921 gtccgaccac cgaaccgctg aaagaagttc agtttaaagc cggtactgac agcctgttcc
    10981 agactggttt tgactttagc gatgcgttgg atgatgacgg tgtttactct tatcgcctga
    11041 ccggtcttgc gcgttctgcc aatgcccagc agaaagggtc agaagagcag cgttatgcta
    11101 ttgcaccggc gttcacctgg cgtccggatg ataaaaccaa tttcaccttc ctttcttact
    11161 tccagaacga gccggaaacc ggttattacg gctggttgcc gaaagaggga accgttgagc
    11221 cgctgccgaa cggtaagcgt ctgccgacag actttaatga aggggcgaag aacaacacct
    11281 attctcgtaa tgagaagatg gtcggctaca gcttcgatca cgaatttaac gacaccttta
    11341 ctgtgcgtca gaacctgcgc tttgctgaaa acaaaacctc gcaaaacagc gtttatggtt
    11401 acggcgtctg ctccgatccg gcgaatgctt acagcaaaca gtgtgcggca ttagcgccag
    11461 cggataaagg ccattatctg gcacgtaaat acgtcgttga tgatgagaag ctgcaaaact
    11521 tctccgttga tacccagttg cagagcaagt ttgccactgg cgatatcgac cacaccctgc
    11581 tgaccggtgt cgactttatg cgtatgcgta atgacatcaa cgcctggttt ggttacgacg
    11641 actctgtgcc actgttcaat ctgtacaatc cggtgaatcc cgatttcgac ttcaatgcca
    11701 aagatccggc aaactccggc ccttaccgca ttctgaataa acagaaacaa acgggcgttt
    11761 atgttcagga tcaggcgcag tgggataaag tgctggtcac cctaggcggt cgttatgact
    11821 gggcagatca agaatctctt aaccgcgttg ctgggacgac cgataaacgt gatgacaaac
    11881 agtttacctg gcgtggtggt gttaactacc tgtttgataa tggtgtaaca ccttacttca
    11941 gctatagcga atcgtttgaa ccttcttcgc aagttgggaa ggatggtaat attttcgcac
    12001 cgtctaaagg taagcagtat gaagtcggcg tgaaatatgt accggaagat cgtccgattg
    12061 tagttactgg tgccgtgtat aatctcacta aaaccaacaa cctgatggcg gaccctgagg
    12121 gttccttctt ctcggttgaa ggtggcgaga tccgcgcacg tggcgtagaa atcgaagcga
    12181 aagcggcgct gtcggcgagt gttaacgtag tcggttctta tacttacacc gatgcggaat
    12241 acaccaccga tactacctat aaaggcaata cgcctgcaca ggtgccaaaa cacatggctt
    12301 cgctgtgggc tgactatacc ttctttgacg gtccgctttc aggtctgacg ctgggcaccg
    12361 gtggtcgtta tactggctcc agctatggtg atccggctaa ctcctttaaa gtgggaagtt
    12421 atacggtcgt ggatgcgtta gtgcgttatg atctggcgcg agtcggcatg gcgggctcca
    12481 acgtggcgct gcatgttaac aacctgttcg atcgtgaata cgtcgccagc tgctttaaca
    12541 cttatggctg cttctggggc gcagaacgtc aggtcgttgc aaccgcaacc tttcgtttct
    12601 aatttctctt ttggggcacg gatttccgtg cccatttcac aagttggctg ttatgcagga
    12661 atacacgaat cattccgata ccacttttgc actgcgtaat atctcctttc gtgtgcccgg
    12721 gcgcacgctt ttgcatccgc tgtcgttaac ctttcctgcc gggaaagtga ccggtctgat
    12781 tggtcacaac ggttctggta aatccactct gctcaaaatg cttggtcgtc atcagcctcc
    12841 gtcagaaggg gagattcttc ttgatgccca gccgctggaa agctggagca gcaaagcgtt
    12901 tgcccgcaaa gtggcttatt tgccgcagca gcttcctccg gcagaaggga tgaccgtgcg
    12961 tgaactggtg gcgattggtc gttacccgtg gcatggcgcg ctggggcgct ttggtgcggc
    13021 agatcgcgaa aaggtcgagg aagctatctc gctggttggc ttaaaaccgc tggcgcatcg
    13081 gctggtcgat agtctctctg gcggcgaacg tcagcgggcg tggatcgcca tgctggtggc
    13141 gcaggatagc cgttgtctgt tgctcgacga accgacttca gcgctggata tcgcccacca
    13201 ggttgatgtg ctggcgctgg tgcaccgttt aagtcaggaa cgtggcctga cggtcattgc
    13261 cgtgttgcac gatatcaata tggcggcacg ctactgtgat tatctggtcg ccctgcgcag
    13321 tggtgaaatg attgctcagg gaacgcctgc ggaaattatg cgcggcgaaa ccctcgaaat
    13381 gatttatggc atcccgatgg gtattttgcc gcatccggcg ggtgctgcac ctgtgagttt
    13441 tgtttattga tgagcggctt acctcttatt tcgcgccgtc gactgttaac ggcgatggcg
    13501 ctttctccgt tgttatggca gatgaatacc gcccacgcgg cggctattga ccccaatcgt
    13561 attgtggcgc tggagtggtt gccggtggaa ttactgctgg cgctcggcat cgtgccttac
    13621 ggcgtggcgg ataccatcaa ctatcgcctg tgggtcagcg aaccaccatt gccggactca
    13681 gtgattgacg tcggtttgcg tacagaacct aaccttgaac tgctgaccga aatgaaaccg
    13741 tcgtttatgg tctggtcggc aggatatggc ccttcatcag aaatgctggc acgtattgcg
    13801 ccgggtcgcg gatttaactt cagtgacggc aaacatccgt tggcgatggc gcgtaaatcg
    13861 ctgacggaaa tggcagattt acttaacctg caaagcgcag cggaaacgca tttagcgcaa
    13921 tacgaagact ttatccgcag catgaaaccc cgctttgtga agcgtggtgc acgcccgtta
    13981 ctgctgacga cgcttatcga tccgcgccat atgctggtct tcggtccaaa cagcttgttc
    14041 caggaaattc tcgatgagta cggcatccca aatgcctggc aaggggaaac caacttctgg
    14101 ggcagtaccg ccgtcagtat cgatcgtctg gcagcgtata aagacgttga tgtgctctgt
    14161 tttgatcacg acaacagcaa agacatggat gcgctaatgg caaccccgct gtggcaggcc
    14221 atgccgtttg tccgtacagg gcgctttcag cgcgtacccg cagtctggtt ttatggtgca
    14281 acgctttcgg caatgcactt tgtgcgtgtt ctggataacg ccatcggagg taaagcgtga
    14341 gtaaacgaat tgcgcttttc ccggcgttat tgctggcgct gttagtgatt gtcgctacgg
    14401 cgctcacctg gatgaacttc tcacaggcgc tgccgcgcag tcagtgggcg caggccgcct
    14461 ggtcgccgga tattgacgtc atcgagcaga tgatttttca ctacagcttg ttgccgcgtc
    14521 tggcgatttc gctgctggtg ggcgcgggcc tggggctggt gggcgtgctg tttcagcaag
    14581 tactgcgtaa cccgctggcg gaaccgacga cgttgggcgt tgcaacaggc gcgcaactgg
    14641 ggattaccgt caccacgctc tgggcgatcc ctagcgcgat ggcgagccag tttgctgcgc
    14701 tggcaggggc ttgcgttgtt ggcttaattg tctttggcgt cgcgtggggg aaacggctgt
    14761 cgccggtaac gctgattttc gcggggttgg tagtgagcct ttattgcggc gcaatcaatc
    14821 agttactggt tatcttccat catgaccagt tgcaaagcat gtttctgtgg agcactggaa
    14881 cgctgacgca aaccgactgg ggctgtgttg agcgtttatg gccgcagttg ctgggcggtg
    14941 tgatgctgac gttgctgcta cttcgtccgt taaccctgat ggggcttgat gatggcgtgg
    15001 cgcgcaatct cgggctggcc ttgtcgcttg cgcgtctggc ggcgctgtcg ctggcgattg
    15061 tcatcagtgc gctgctggtg aacgctgtgg ggattatcgg ctttatcggg ttgttcgcgc
    15121 cgctgctggc aaaaatgctg ggggcgcggc gtctgctgcc acgactgatg ctggcgtcgt
    15181 tgattggtgc gctgatcctc tggctttccg atcaaatcat cctctggctg actcgcgtgt
    15241 ggatggaagt gtccaccggt tcggtcactg cgttgatcgg tgcgccgctg ctactgtggc
    15301 tgctgccgcg tttacgcagc attagcgcgc cggatatgaa ggttaacgat cgtgtcgcga
    15361 ctgaacgcca acatgtgctg gcgtttgccc tcgcgggcgg cgtgctgttg ttgatggctg
    15421 tggtggtggc gctgtcgttt ggtcgtgatg cgcacggctg gacgtgggcg agcggggcgt
    15481 tgctcgagga tttaatgccc tggcgctggc cgcgaattat ggcggcgctg tttgcgggcg
    15541 tcatgctggc ggtagcgggc tgtattattc agcgactgac cggcaacccg atggcaagcc
    15601 cggaagtgct ggggattagc tccggcgcgg cgtttggtgt ggtgttaatg ctgtttctgg
    15661 taccgggtaa tgcctttggc tggttgttac ccgcaggcag tctcggggcg gcggtgacgc
    15721 tgttgatcat tatgatcgcc gccggacgcg gtggattttc cccacaccgc atgttactgg
    15781 cggggatggc gttaagtacc gcgttcacca tgcttttgat gatgttgcag gcaagtggtg
    15841 acccgcgaat ggcgcaagtg ctgacctgga tttccggttc gacctacaac gcgaccgatg
    15901 ctcaggtctg gcgcaccgga attgtgatgg tgattttgct ggcgattact ccgctgtgcc
    15961 gccgctggct gaccatttta ccgctgggtg gtgataccgc gcgggcggta gggatggcgc
    16021 tgacgccgac gcgaattgcg ctgctgctgt tagcggcttg cctgacggcg accgcgacga
    16081 tgactattgg accgttgagt tttgttggtt taatggcacc gcatattgcg cggatgatgg
    16141 gctttcgacg gacgatgcca cacatcgtaa tttcggcgct ggtgggtggt ttactgctgg
    16201 tgttcgctga ctggtgtggg cggatggtgc tgtttccatt ccagatcccg gcggggctgc
    16261 tgtcgacctt tatcggtgcg ccatatttta tctatttgct gagaaagcag agccgttaat
    16321 agaatgcctc gcccggtggg cgaggcaaaa ttatcacaac ttcgcgaaca cccgacgtgc
    16381 agcatcgatg gtgttattga tatcttccat gctgtgcgcc acagacataa agcctgcttc
    16441 aaacgctgac ggtgccaggt aaacaccttc atccagcatc atatggaaga aacgcttaaa
    16501 acgctcgacg tcacaggcca tcacatcctg atagcacgtc acggactcgg cgtcggtaaa
    16561 gaagataccg aacataccac cgacgtggtt aacgaccagc ggaattcccg cttcttctgc
    16621 cgcttccagc agaccttctg ccagacgtgt tgtcagctca tccagcgttt cgtgaatgcc
    16681 cggctgcgcg acttcattca gacaggcgaa acccgctgcc atcgcaatcg ggttaccgga
    16741 aagcgtaccc gcctgataga ccggacctgt cggggccagc gcatccatta catcacgacg
    16801 accaccgaat gcgcctaccg gcattccacc gccgatgatt tttccgaggc aggttaaatc
    16861 tggcactacg gcgaaataat cctgtgcgcc agctagcgct acgcggaaac cggtcatcac
    16921 ttcatcgatg atcagcaacg cgccaaattc gtcgcacagc gcacgcagac ctggcaggaa
    16981 ctctggcagc ggcggaacac agttcatatt gcctgccacc ggctcgacga taatacaggc
    17041 aatctcttgc gggtattgct caaacgcggc gcgtacagag gccagatcgt tataagtaca
    17101 ggttaaggta tgtttggcga aatctgccgg aacgcccggc gagtttggct ggcctaacgt
    17161 gagtgcgcca gaaccggctt tcaccagcag gcagtcagcg tgaccgtggt aacaaccttc
    17221 aaatttaatg attttgtcgc gaccggtaaa accacgggcc aggcggatgg cgctcatcgt
    17281 cgcctcggtg ccggagttca ccatgcgcac catatccatg gtcgggacca gttcggtcac
    17341 cagttgcgcc attttcactt ccatttcggt cggtgcacca aagcttaaac cacgctcggc
    17401 ggcttcaatc acggcattgc ggatcgccgg atggttatgg cccagcacca tcggccccca
    17461 ggaaccgaca taatcgatat aggctttgcc atcaacatcg tacagataag cgccgtccgc
    17521 tttttctata aacagtggag tgccgcccac gccagtaaag gcgcgaacag gggagttcac
    17581 accgccaggg atcagctcgc gcgctgcgct gtaaagattt tcagacttac tcatggaggg
    17641 ttcctgattc gtagaaaaag tgaatggctg ctattccatg ttattcataa caagttaaat
    17701 actcgtcaaa catcaggctg cttgtactgg tgcaatcctg atttcttaga gtataaaagt
    17761 tttgtgcatt tgaaacatta cgctttgcaa aggattttca tggaacgtgc gagtaaaatg
    17821 ccgtcatctt atttgtatga ccaataagtg atcattggat gaaaactgat actccctctt
    17881 tagaaacacc gcaggccgcg cgcctgcgac gcagacaact gattcgccaa cttcttgagc
    17941 gcgataaaac cccgttagcc attttgttta tggcggcagt cgtcggcacg cttgtcgggc
    18001 tggctgcggt tgcttttgac aaaggtgtcg cctggttgca gaaccaacgt atgggggcgc
    18061 tggtacatac tgctgataat tatccgctac tgttaaccgt cgcttttctc tgttcggcgg
    18121 tgctggcgat gttcggctac tttttggtgc gcaaatacgc gcccgaagca gggggatcgg
    18181 ggatcccgga aattgaaggg gcgctggaag atcaacgtcc tgttcgctgg tggcgtgtat
    18241 tgccggtgaa attctttggc gggctgggga cactcggcgg aggcatgggg ctagggcgcg
    18301 aaggaccaac cgtgcagatc ggcggcaata tcagtcgcat ggtgcttgat attttccgcc
    18361 tgaaaggtga tgaagctcgc catacgctgc tggcaaccgg tgctgctgcg gggctggctg
    18421 cggcctttaa cgcgccgctg gcgggtattt tgtttattat cgaagagatg cgtccgcagt
    18481 ttcgctatac gttaatttcg attaaagcgg tatttattgg cgtcattatg tcgaccatta
    18541 tgtaccggat ttttaatcat gaagttgcgt tgattgacgt cggtaaactt tctgatgcgc
    18601 cgcttaatac gctgtggctt tatctgatcc tcggtattat ttttggcatt ttcggcccta
    18661 tttttaataa atgggtgctg gggatgcagg atttgctgca ccgtgtgcac ggcggcaata
    18721 ttaccaaatg ggtactgatg gggggggcga ttggcggtct gtgtggattg ctggggtttg
    18781 tggcaccagc aacgtcgggc ggcggtttta acctgattcc tatcgctacc gcggggaatt
    18841 tcagcatggg aatgctggtg tttatcttcg tcgctcgggt cattaccacc ttactctgct
    18901 tctcttccgg cgcgccgggc ggtatttttg ccccgatgct ggcgctgggt actgtgctgg
    18961 gaaccgcttt cggaatggtt gccgttgagc tgtttccgca atatcacctg gaggcgggga
    19021 cgtttgctat tgccggaatg ggggcattac tggcggcatc tattcgcgcg ccgttaacgg
    19081 ggatcattct ggttctggag atgaccgata actaccagct cattttgcca atgattatta
    19141 ccggtcttgg cgcaacacta ttagcgcaat ttaccggcgg gaaacctcta tactcggcga
    19201 ttcttgcgcg cacgctggca aaacaggaag ctgagcaact ggcgcgaagc aaggccgcat
    19261 cagccagcga gaatacttga acgaaatacc agggtattag ataatggcga ttattattgg
    19321 gttagaattt gcccgattgc cgatgtcgtt tggagcaaaa tatgagtgat gacgtagcac
    19381 tgccgctgga gtttaccgac gcagcagcca acaaagttaa aagcctgatc gctgacgaag
    19441 ataacccgaa tctgaaatta cgcgtgtata tcaccggtgg cggttgcagc ggcttccagt
    19501 atggtttcac ctttgatgat caggtgaacg aaggcgatat gaccatcgaa aaacagggcg
    19561 ttggcctggt ggttgatccg atgagcctgc aatatctggt cggcggttcc gttgactaca
    19621 ccgaaggact ggaaggttct cgtttcatcg tgaccaaccc gaacgcgaaa agcacctgcg
    19681 gttgcggttc ttcctttagc atctaatctg ttgttcatgt gccggataaa ctatccggca
    19741 catcttctct cttcaacgcc cattctcatc cagcgcaaac gtcggtagct taagatgcca
    19801 acgaatagcc gccagccgaa tcaatagcgt cacgaccatg cccatcatac tggctgtttc
    19861 cagtggtacg gaaaatgtgt aataagccgt agcgtggaca ataccgccga taatacaggc
    19921 agttgcgtag atttctgtac gtaaaatcat ggggatttcg cggaccagta catcacgaat
    19981 gatcccgccg ccaacgccag taatgacgcc catacaaacc gcgattaacg gaccggcttc
    20041 cgcattaaag gctttattca cgctaatgcc gacaaacacc gccagaccaa cggcgtccag
    20101 caccggcaac atccattttg gtaagcgtct tggctggcgc accagcacga tggtcagcat
    20161 gctggtgacc atggcaacga ccagatcggt gggatctttc acccaaaata ccgggccgtg
    20221 atccagcgcc atgtcgcgaa ttgttccgtc gcctactgcg gtaaccacgc ccagtaccag
    20281 aacaccaaaa gggtccatac gcaattttcc ggctaacaaa acgccgtaga tggcaaatac
    20341 cgctgtgccg actatatcca gccaatagac gagcattgtt caatccccac tgagcacctg
    20401 aaaaggtcag gcactaatct acctgtgaaa gcgcattaca gagctgttgt gctgcgagga
    20461 taatacgtgg gcttgcacgt tcaaaccagt cactcgtgag aggaataacg ggaattttga
    20521 gctgttcacc ccagtattgt ttgattttag gaatttggtc cggtccgcct gtaatgacaa
    20581 tagcctgtgg cgagcgtgct aacacctgtt cgcggctaac ttgcggccag ggaacccggc
    20641 tgtctttaaa gatgttttct ccgccacaaa cttcgagtac ctggttctga atcgactctt
    20701 ttccactggt aaatggcgga ttaatgccga attgcagaaa aacacgtttt ttaggtttat
    20761 cagcatattg cgctttcaat tgcacgtact gatccagcag ggattgcgcg gcttgttcgg
    20821 ccttgtctgg ttgcggactc cagggggcca gttgacgtaa cgcattggca atttgttcaa
    20881 tgcttgtcgc atcgacccac atcactttta ttcccagcga agccagctgg tcaacctgcc
    20941 gctcggcatt acctccacgc caggcaatca ccagatcggg tttcagcgcg acaatgcgtt
    21001 ccagattcat cccctgccag gtggaaacct gctcaatctt ttgcgcttgt ggaggatagt
    21061 cggaatagct gctgacccca accggcgtga tcccggcggc aaaggcaagt tcagtgttgg
    21121 cgggagaaag cgtgatgacg cgcggcgcgg cgttgagcca cagtggcgcg agaaaagaca
    21181 gggcgaccag cgccctgaac agtgatttag ccatgtgcca gtttctgcac cagtgactca
    21241 accatcaggc tggactgttt agcggcaaca gccaggaact catcgaagct aagatgagac
    21301 tgttgatcgg ccacgtcgga gatggcgcgt acgacaacaa acgggacgtt gaaattgtgg
    21361 cagacatggg cgattgccgt cgcttccatc tctacagcaa tggcctgtgg gaagttgtgg
    21421 cggattttcg ccagaccaac agaaccgttg atgaaagcgt cgccgctaac aatcaggcca
    21481 cgtacagcgt taagattcag ttcggcaatg caggcctcag cggcagcgat cagtttatcg
    21541 tcagctttaa agcctgccgg acagcctggt aactgaccgt attcataacc aaatgccgtg
    21601 acatccgcgt cgtgataacg cgcttcgtcc gagacaacga tatcgcccac tttcagcgtt
    21661 ggtgccaggc caccggcaga accggtgtta ataatcacat ctggcttgca gtgttccaac
    21721 agcagagtgg cacccagcgc cgcagcgact ttaccgatgc ccgatttcag aagcgcaacc
    21781 tcggttccat tcagttggcc ggtatagatt tcgcaaccgc cgagactgat agtttgacgg
    21841 ttttcgattt tgtcacgcag cagcgtaact tcttcttcca ttgcaccaat gatgccgatt
    21901 ttcatagatt tactcgcgat aagcccgatt tgaaggcata gtttaccatg cgcttacggg
    21961 gaagcgtatt tctcacgcgg gagaggacat ggcacagatt gatttccgaa aaaaaataaa
    22021 ctggcatcgt cgttaccgtt caccgcaggg cgttaaaacc gaacatgaga tcctgcggat
    22081 cttcgagagc gatcgcgggc gtatcatcaa ctctccggca attcgtcgtc tgcaacaaaa
    22141 gacccaggtt tttccactgg agcgcaatgc cgccgtgcgc acgcgtctta cccactcgat
    22201 ggaagtccag caggtgggac gctacatcgc caaagaaatt ttaagccgtc tgaaagagct
    22261 taaattgctg gaagcatacg gcctggatga actgaccggc ccctttgaaa gcattgttga
    22321 gatgtcatgc ctgatgcacg atatcggcaa tccgccgttt ggtcattttg gcgaagcagc
    22381 gataaatgac tggtttcgcc aacgtttgca cccggaagat gccgaaagcc agcctctgac
    22441 tgacgatcgc tgcagcgtgg cggcactacg tttacgggac ggggaagaac cgcttaacga
    22501 gctgcggcgc aagattcgtc aggacttatg tcattttgag gggaatgcac aaggcattcg
    22561 tctggtgcat acattgatgc ggatgaatct cacttgggca caggttggcg gtattttaaa
    22621 atatacccgt ccggcgtggt ggcgtggcga aacgcctgag acacatcact atttaatgaa
    22681 aaagccgggt tattatcttt ctgaagaagc ctatattgcc cgcttgcgta aagaacttaa
    22741 tttggcgctt tacagtcgtt ttccattaac gtggattatg gaagctgccg acgacatctc
    22801 ctattgtgtg gcagaccttg aagatgcggt agagaaaaga atatttaccg ttgagcagct
    22861 ttatcatcat ttgcacgaag cgtggggcca gcatgagaaa ggttcgctct tttcgctggt
    22921 ggttgaaaat gcctgggaaa aatcacgctc aaatagttta agccgcagta cggaagatca
    22981 gttttttatg tatttacggg taaacaccct aaataaactg gtaccctacg cggcacaacg
    23041 atttattgat aatctgcctg cgattttcgc cggaacgttt aatcatgcat tattggaaga
    23101 tgccagcgaa tgcagcgatc ttcttaagct atataaaaat gtcgctgtaa aacatgtgtt
    23161 tagccatccg gatgtcgagc agcttgaatt gcagggctat cgggtcatta gcggattatt
    23221 agagatttat cgtcctttat taagcctgtc gttatcagac tttactgaac tggtagaaaa
    23281 agaacgggtg aaacgtttcc ctattgagtc gcgcttattc cacaaactct cgacgcgcca
    23341 tcggctggcc tatgtcgagg ctgtcagtaa attaccgtca gattctcctg agtttccgct
    23401 atgggaatat tattaccgtt gccgcctgct gcaggattat atcagcggta tgaccgacct
    23461 ctatgcgtgg gatgaatacc gacgtctgat ggccgtagaa caataaccag gcttttgtaa
    23521 agacgaacaa taaattttta ccttttgcag aaactttagt tcggaacttc gggctataaa
    23581 acgaatctga agaacacagc aattttgcgt tatctgttaa tcgagactga aatacatgaa
    23641 aaaaaacaca ttagcactga gtgcactggc tctgagttta ggtttggcgt tatctccgct
    23701 ctctgcaacg gcggctgaga cttcttcagc aacgacagcc cagcagatgc caagccttgc
    23761 accgatgctc gaaaaggtga tgccttcagt ggtcagcatt aacgtagaag gtagcacaac
    23821 cgttaatacg ccgcgtatgc cgcgtaattt ccagcagttc ttcggtgatg attctccgtt
    23881 ctgccaggaa ggttctccgt tccagagctc tccgttctgc caggggggcc agggcggtaa
    23941 tggcggcggc cagcaacaga aattcatggc gctgggttcc ggcgtcatca ttgatgccga
    24001 taaaggctat gtcgtcacca acaaccacgt tgttgataac gcgacggtga ttaaagtcca
    24061 gctgagcgat ggtcgtaagt tcgacgcgaa gatggttggc aaagatccgc gctctgatat
    24121 cgcgctgatc caaatccaga acccgaaaaa actgaccgca attaagatgg cggattctga
    24181 tgcactgcgc gtgggtgatt acaccgtagc gattggtaac ccgtttggtc tgggcgagac
    24241 ggtaacttcc gggattgtct ctgcgctggg gcgtagcggc ctgaatgctg aaaactacga
    24301 aaacttcatc cagaccgatg cagcgatcaa ccgtggtaac tccggtggtg cgctggttaa
    24361 cctgaacggc gaactgatcg gtatcaacac cgcgatcctc gcaccggacg gcggcaacat
    24421 cggtatcggt tttgctatcc cgagtaacat ggtgaaaaac ctgacctcgc agatggtgga
    24481 atacggccag gtgaaacgcg gtgagctggg tattatgggg actgagctga attccgaact
    24541 ggcgaaagcg atgaaagttg acgcccagcg cggtgctttc gtaagccagg ttctgcctaa
    24601 ttcctccgct gcaaaagcgg gcattaaagc gggtgatgtg atcacctcac tgaacggtaa
    24661 gccgatcagc agctttgccg cactgcgtgc tcaggtgggt actatgccgg tgggcagcaa
    24721 actgaccctg ggcttactgc gcgacggtaa gcaggttaac gtgaacctgg aactgcagca
    24781 gagcagccag aatcaggttg attccagctc catcttcaac ggcattgaag gcgctgagat
    24841 gagcaacaaa ggcaaagatc agggcgtggt agtgaacaac gtgaagacgg gcactccggc
    24901 tgcgcagatc ggcctgaaga aaggtgatgt gattattggt gcgaaccagc aggcagtgaa
    24961 aaacatcgct gaactgcgta aagttctcga cagcaaaccg tctgtgctgg cactgaacat
    25021 tcagcgcggc gacagcacca tctacctgtt aatgcagtaa tctccctcaa ccccttcctg
    25081 aatacgggaa ggggttctcc ttacaatctg tgaacttcgc cacaactcca tacatcttca
    25141 tcatcctttg ggcatttgca caatgccgta cgttacgtac ttccttatgc taagccgtgc
    25201 ataacggagg acttatggct ggctggcatc ttgataccaa aatggcgcag gatatcgtgg
    25261 cacgtaccat gcgcatcatc gataccaata tcaacgtaat ggatgcccgc gggcgaatta
    25321 tcggcagcgg cgatcgtgag cgtattggtg aattgcacga aggtgcattg ctggtacttt
    25381 cacagggacg agtcgtcgat atcgatgacg cggtagcacg tcatctgcac ggtgtgcggc
    25441 aggggattaa tctaccgtta cggctggaag gtgaaattgt cggcgtaatt ggcctgacag
    25501 gtgaaccaga gaatctgcgt aaatatggcg aactggtctg catgacggct gaaatgatgc
    25561 tggaacagtc gcggttgatg cacttgttgg cgcaggatag ccgtttgcgg gaagaactgg
    25621 tgatgaacct gattcaggca gaggagaata ctcccgcact tctgaatggg cgcaacggct
    25681 ggggatcgat ctcaatcaac cgcgagtggt ggctattgtt gaggtcgaca gcggtcagct
    25741 tggcgtggac agcgcaatgg cggagttaca acaactgcaa aacgcgctga ctacgcccga
    25801 gcgtaataat ctggtggcga ttgtctcgct aaccgaaatg gtggtgttga aaccggcgtt
    25861 gaactctttt gggcgctggg atgcagaaga tcatcgtaag cgagttgaac aactgattac
    25921 ccgcatgaaa gagtacggcc agctgcgttt tcgcgtttca ctgggcaact attttaccgg
    25981 tcctggcagt attgcccgat cctatcgtac ggcgaaaacg acgatggtgg tgggtaaaca
    26041 gcggatgcca gaaagtcgct gctattttta tcaggatctg atgttacctg tgttactcga
    26101 cagtttgcgt ggcgactggc aggccaacga actggcgcga ccgctggcgc ggctgaaagc
    26161 gatggacaat aacggcttgc tgcgacgaac gctggcggcg tggtttcgcc acaatgtgca
    26221 accgctggca acgtcaaagg cgttgtttat tcatcgtaat accctggagt atcggcttaa
    26281 tcgtatatcg gaactgaccg ggcttgattt gggcaatttt gatgacaggt tgctgctgta
    26341 tgtggcgtta caactggatg aagagcggta gattatgcgt taaggttgag gcggcgctgg
    26401 ctcattcccc tcagcccgaa ggggagaggg gaatgcccga gttaaccgtc aattcgggat
    26461 tatttacgcg ttagtttttc cagatcggct tcaatctcgc tgatcttatt ggttacgact
    26521 gactccaggt gacgtaagtc gtcgaggatc ttacgcttaa gatcaacttc gctgcggtca
    26581 cgctgacaga tttgatcaag ctcatcaatg atataccgta gattcgggct gatttcctgg
    26641 acttctttat aaccctgacc cacaccatca gcgacgaccg ttttacgctg acgcggatat
    26701 ttaaacttaa cgctcttggc gaaaaactcg cctttgtctt tctggaaata gattttcaga
    26761 atatcgttgt tggcttcctg ccggaggctg taacgatcaa tttcttcagg attggtaata
    26821 cccagacttt tcagattgtc gtacatagcg ttacctcaaa atgagtcagt aaattgtgct
    26881 tattttagca tttggcttgg cccgcccggc tcgactgtga tcgcagaaag cctttttctt
    26941 ccagacttct atcatggcgt aaaaatcaaa aaattacctg ctttattgtg gcgatattcc
    27001 atgggatgga ttagcacatt tatcttcgtt ttgcgaatta catgacagtc cgaaacaaga
    27061 tgaaaaagta tttaaaaaag ggggaggaag aatgagtagg acgaattatt ctgagccaga
    27121 aagcatacgg gcagcatcat gctgcccgta tatgtactct taatcgatgg tacgcagcag
    27181 ttcgttaatg ccgactttgc cgcgagtttt cgcgtcaact ttcttaacga taaccgcaca
    27241 gtagaggctg tatttgccat cttttgacgg cagattacct gaaacaacca cagaccccgc
    27301 cggaacgcga ccgtagtgga tttcgccggt ttcacggtcg taaatacggg tgctctgacc
    27361 aatgtatacg cccatagaga tgacggaacc ttcttcgaca atcacccctt caaccacttc
    27421 agagcgtgcg ccaatgaagc agttatcttc aataatggtc gggttagcct gcagcggctc
    27481 cagcacgccg ccgatgccca cgccaccgga aaggtggacg tttttaccaa tctgcgcaca
    27541 agaaccgacg gtcgcccagg tatcaaccat ggtgccttca tcaacatatg cgccgatgtt
    27601 aacgtaagac ggcatcagca cggtgttacg ggcaataaac gcaccctgac gtaccgccgc
    27661 tggtggcaca acgcggaagc cttctttctg gaaacgtgct tcgtcgtagt cggcgaattt
    27721 catcggcact ttgtcgaagt agcggctttc tgccccttcg atcacctgat tatcattaat
    27781 acggaaagag agcagcaccg cttttttcaa ccactgatgc gtcacccact gaccgtcaat
    27841 tttttccgct acacgcagtg cgccggaatc cagcagggcg atcacctgat ttaccgcttc
    27901 gcgggtaacg gtgtctgcat tggctggcgt gatctcggca cggcgttcaa aagcggtttc
    27961 aataatgttc tgtaactgct gcattgttaa actcttttca tatcagtaaa cacatcaccc
    28021 tttatcgttt ggattgaggg cctctgtcaa ccgctgatgc acttcctgct gcaactcgtt
    28081 attaagcgca cgccggtcag cggtggcaat tatgaataaa tcttctactc gctcgccaat
    28141 ggttgtaatt cgggcaccat gaagcgaaat tcccagatcg gcaaaaattt tcccgactcg
    28201 cgccagcagt ccaggttggt cgagggcgat cagttcgagg aacgatttgc ggtcggtatg
    28261 ggtcggcaaa aacgttactt cggtttcaac agtaaaatgg cgtaatttgg cgggttggcg
    28321 acggggctgc ggtggctgcc agctactttg cgtcagcacc tgctccagac caaaccgaat
    28381 aacctcatgg cgatctgcgg acagcgggct gccatcgggt tccagcacga taaaggtatc
    28441 catcgccata ccgtcgcgag tggtgaaaat ttgtgcgtcg tgaacactta aattgcggcg
    28501 gtctaattcg gcacagacgg cggcaaacag ataggggcgg tccgggctcc agataaaaat
    28561 ctcggtgcct ccacgcgtag cctgcgggct aagcaatacc agcggtttgc ttaaatcatg
    28621 ctgtaataaa tggcgggcat gccaggccag ttgatttggg ctatggcgga caaaatagtt
    28681 agcacgacag cgtgaccaaa tttggtgcag cgcctcttcg tcgatgttat ccatgcgcag
    28741 tagtgccagt gcctggagtt ggtgatggcg aacccgttcg cgcatatccg gcgtgttttg
    28801 cattccgcgt cgtagctgct tttcggtggc aaaatagagt tcgcgcaaca gactttgctt
    28861 ccagctattc cacagtgttt cgttggtggc gcaaatgtca gccactgtca ggcataccag
    28921 atagcgcaga cgattttccg tttgaacttc ttcggcaaac tgcttgatga cttcagggtc
    28981 ctgaatatcg cggcgttgtg ctgtcaccga catcagcaag tgctggcgaa ccagccaggc
    29041 aaccagctgt gtttcgcgtg agttcagccc gtggagttct gcaaaatgca ctacatcctg
    29101 agcaccgaga atggagtggt cgccgccgcg tcctttggcg atatcgtgaa acagcgcggc
    29161 gatgaaaatc agctcagttg acggcaggcg cggccagacg tccacacaca acggatggcg
    29221 ctggcgcgtt tcttcactgg caaaactctc cagtttcagc atcacgcgga tagtatgttc
    29281 atccaccgtg taggcgtgga acagatcaaa ctgcatctgc ccgacgatat gcgaccattg
    29341 cggcatatac gcgccgagca cgctatggcg atgcattggc aatagtccgc gccgcaccgc
    29401 tccggggtga cgcagaatgc tcaaaaacag ttttcgtgct tgcggaatat tacacagcgg
    29461 ttgttgcaga tggcgacggg catggcgtaa ctggcgcagc gtggtggagt aaatgccggt
    29521 gatcgcactg ttgcgcacca tggtgtagaa catacgcaag atggcttccg gctggcgcat
    29581 aaatagtgtt tcatcacgca ggtcgattag cgtaccgcgt agctgaaact catcgtcgag
    29641 tggacgtggt ttttcgtcgg cgggaagggc gaggatggct tcatcgaaca gttgcagcag
    29701 catctggttg agttcactga cgcggcgtgt aacgcggaag taatccttca tcatccgctc
    29761 gaccggttcg ttgccttcac cactgtaatt cagacgctga gcgacgctaa gttggcgatc
    29821 gaataacaga cgattatcgt aacggctgac gaccagatgc agggcaaagc gaatacgcca
    29881 cagtatatgc agacattcgt ttaattccgc ccgctccgct gaggttaaga agccaaaccc
    29941 gaccatttca tccagcgatg ttgcgccaaa atgacggcgg gccacccatt gcagagtgtg
    30001 aatatcgcgc aagccgccag ggctactttt gatgtctggt tcaaggttgt agctggtgcc
    30061 atggtaacgc tgatggcgct ggttctgttc ttcaactttc gctgcgtaga acttgtcgga
    30121 aggccagaat ccttcgctaa aaatatgttt ttgcagttcg aggaagagcg caacatcgcc
    30181 aattaataag cgggattcga ttaaattggt ggcgacggtt aaatccgata acccttccaa
    30241 catgcactct tcaagcgtgc gcacactatg accgacttcc agctttacat cccagagcag
    30301 cgttaacagc tcgcccactt tttgcgcctg atcgtccggg agctttttac ggcttaaaat
    30361 cagtaaatcg atgtcagaaa gtggatgcag ctcgccacga ccgtagccac cgacggcaac
    30421 caatgccagg tcggcaatct ggctgaatcc cgcttcaatc cataatcgtt gcaggagctg
    30481 gtctatgaac tcggtacgtg cctcaatcaa ctgttctgca gagatcccat tgtcaaaggc
    30541 atcacccagc caacgctgga aagtatcgat atgggctttt atcccacaga cggttaattc
    30601 atcacggggc caggcgcatg gattttgcgg ttgaccgggc agggtggaga gagcggtgtt
    30661 tgcgtactgt tctggaaggg tattcattgt gcgccaccca taagattaaa ttatcgcatt
    30721 aaaaaagccg gcattcgccg gcttcatctt attcgtcgtg cgagattatc gccgggatgg
    30781 tgtcatcctt gcgtagcgtc aaaatttcgc agccgttatc agtcaccaca atagtatgct
    30841 catattgtgc agacaagctg cgatctttgg tttttaccgt ccagccatct ttcatggtgc
    30901 ggatctcttt tttacccgcg ttgaccattg gctcaatggt gaacgtcatc cccggtttca
    30961 gcacgacgtt ggtttcacgg gagtcatagt gcagcacctg cggttcttca tggaagccgc
    31021 gaccaatacc gtgtccgcaa tattcacgaa cgacggagaa gccttctgct tcgacaaatt
    31081 tctgaatcgc cgcaccgatt tcgcgcagat taatgcctgg ttttaccatg cgtagcgcca
    31141 ggtacaggct ttcttgcgtg atgcggcaca ggcgttcgcc catgatggtc ggcttaccga
    31201 cgataaacat tttcgaggta tcgccgtgga aaccatcttt gattacggtg acatcaatgt
    31261 taacgatatc gccatctttc agcagcttag catcgtccgg gataccgtgg cacaccactt
    31321 cattaataga gatgcaaacg gatttcggat agccgtgata gccgaggcag gcagaaaccg
    31381 cgtgttgttc attaacaatg taatcattac agatgcgatc cagctcgccg gtgctgacgc
    31441 ccggtttaac atacggttcg atcatctcca gcacttcggc agccagtcgg ccagcgacgc
    31501 gcattttttc gatatcttct ggggtcttga ttgagatagc cattaattct gtccatcagc
    31561 gtcggtgata ccgacaatat atatgtaagt gccgtcaatg gtatcacacc cgggcaaatt
    31621 gagaatcatt ctgaatttcg ccaaacgtgc cactgaaggt tttctataat agaaaattcg
    31681 acgtctgatg ctgtacacag cgccaacaat tattggtgtc cacgacgtat ttgtggtata
    31741 aagcgcgccg gacttccgat ccatttcgta tacacagact ggacggaagc gacaatctca
    31801 ctttgtgtaa caacacacac gtatcggcac atattccggg gtgccctttg gggtcggtaa
    31861 tatgggatac gtggaggcat aaccccaact tttatataga ggttttaatc atggcaactg
    31921 tttccatgcg cgacatgctc aaggctggtg ttcacttcgg tcaccagacc cgttactgga
    31981 acccgaaaat gaagccgttc atcttcggtg cgcgtaacaa agttcacatc atcaaccttg
    32041 agaaaactgt accgatgttc aacgaagctc tggctgaact gaacaagatt gtttctcgca
    32101 aaggtaaaat ccttttcgtt ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg
    32161 ctctgagctg cgaccagttc ttcgtgaacc atcgctggct gggcggtatg ctgactaact
    32221 ggaaaaccgt tcgtcagtcc atcaaacgtc tgaaagacct ggaaactcag tctcaggacg
    32281 gtactttcga caagctgacc aagaaagaag cgctgatgcg cactcgtgag ctggagaaac
    32341 tggaaaacag cctgggcggt atcaaagaca tgggcggtct gccggacgct ctgtttgtaa
    32401 tcgatgctga ccacgaacac attgctatca aagaagcaaa caacttgggt attccggtat
    32461 ttgctatcgt tgataccaac tctgatccgg acggtgttga cttcgttatc ccgggtaacg
    32521 acgacgcaat ccgtgctgtg accctgtacc tgggcgctgt tgctgcaacc gtacgtgaag
    32581 gccgttctca ggatctggct tcccaggcgg aagaaagctt cgtagaagct gagtaataag
    32641 gcttgataac tcccccaaaa tagttcgagt tgcagaaagg cggcaagctc gagaattccc
    32701 gggagcttac atcagtaagt gaccgggatg agcgagcgaa gacaacgcat ctgcggcgcg
    32761 aaatatgaag ggggagagcc cttatagacc aggtagtaca cgtttggtta gggggcctgc
    32821 atatggcccc ctttttcact tttatatctg tgcggtttaa tgccgggcag atcacatctc
    32881 cgaggatttt agaatggctg aaattaccgc atccctggta aaagagctgc gtgagcgtac
    32941 tggcgcaggc atgatggatt gcaaaaaagc actgactgaa gctaacggcg acatcgagct
    33001 ggcaatcgaa aacatgcgta agtccggtgc tattaaagca gcgaaaaaag caggcaacgt
    33061 tgctgctgac ggcgtgatca aaaccaaaat cgacggcaac tacggcatca ttctggaagt
    33121 taactgccag actgacttcg ttgcaaaaga cgctggtttc caggcgttcg cagacaaagt
    33181 tctggacgca gctgttgctg gcaaaatcac tgacgttgaa gttctgaaag cacagttcga
    33241 agaagaacgt gttgcgctgg tagcgaaaat tggtgaaaac atcaacattc gccgcgttgc
    33301 tgcgctggaa ggcgacgttc tgggttctta tcagcacggt gcgcgtatcg gcgttctggt
    33361 tgctgctaaa ggcgctgacg aagagctggt taaacacatc gctatgcacg ttgctgcaag
    33421 caagccagaa ttcatcaaac cagaagacgt atccgctgaa gtggtagaaa aagaatacca
    33481 ggtacagctg gatatcgcga tgcagtctgg taagccgaaa gaaatcgcag agaaaatggt
    33541 tgaaggccgc atgaagaaat tcaccggcga agtttctctg accggtcagc cgttcgttat
    33601 ggaaccaagc aaaactgttg gtcagctgct gaaagagcat aacgctgaag tgactggctt
    33661 catccgtttc gaagtgggtg aaggcatcga gaaagttgag actgactttg cagcagaagt
    33721 tgctgcgatg tccaagcagt cttaattatc aaaaaggagc cgcctgaggg cggcttcttt
    33781 ttgtgcccat cttgtaaatt cagctaaccc ttgtggggct gcgctgaaaa gcgacgtaca
    33841 atgtcgctag tattaattca tttcaatcgt tgacagtctc aggaaagaaa catggctacc
    33901 aatgcaaaac ccgtctataa acgcattctg cttaagttga gtggcgaagc tctgcagggc
    33961 actgaaggct tcggtattga tgcaagcata ctggatcgta tggctcagga aatcaaagaa
    34021 ctggttgaac tgggtattca ggttggtgtg gtgattggtg ggggtaacct gttccgtggc
    34081 gctggtctgg cgaaagcggg tatgaaccgc gttgtgggcg accacatggg gatgctggcg
    34141 accgtaatga acggcttggc aatgcgtgat gcactgcacc gcgcctatgt gaacgctcgt
    34201 ctgatgtccg ctattccatt gaatggcgtg tgcgacagct acagctgggc agaagctatc
    34261 agcctgttgc gcaacaaccg tgtggtgatc ctctccgccg gtacaggtaa cccgttcttt
    34321 accaccgact cagcagcttg cctgcgtggt atcgaaattg aagccgatgt ggtgctgaaa
    34381 gcaaccaaag ttgacggcgt gtttaccgct gatccggcga aagatccaac cgcaaccatg
    34441 tacgagcaac tgacttacag cgaagtgctg gaaaaagagc tgaaagtcat ggacctggcg
    34501 gccttcacgc tggctcgtga ccataaatta ccgattcgtg ttttcaatat gaacaaaccg
    34561 ggtgcgctgc gccgtgtggt aatgggtgaa aaagaaggga ctttaatcac ggaataattc
    34621 ccgtgatgga taaataaggg taagattccg cgtaagtatc gcgggggcgt aagtctggtt
    34681 ataaggcgtt attgttgcag gcagtttggt cacggccagc gcgcagcaac cggagcgtac
    34741 aaaagtacgt gaggatggcg agcactgctc ggggccaaaa tggcaaataa aatagcctaa
    34801 taatccagac gattacccgt aatatgttta atcagggcta tacttagcac acttccactg
    34861 tgtgtgactg tctggtctga ctgagacaag ttttcaagga ttcgtaacgt gattagcgat
    34921 atcagaaaag atgctgaagt acgcatggac aaatgcgtag aagcgttcaa aacccaaatc
    34981 agcaaaatac gcacgggtcg tgcttctccc agcctgctgg atggcattgt cgtggaatat
    35041 tacggcacgc cgacgccgct gcgtcagctg gcaagcgtaa cggtagaaga ttcccgtact
    35101 ctgaaaatca acgtgtttga tcgttcaatg tctccggccg ttgaaaaagc gattatggcc
    35161 tccgatctcg gcctgaaccc gaactctgcg ggtagcgaca tccgtgttcc gctgccgccg
    35221 ctgacggaag aacgtcgtaa agatctgacc aaaatcgttc gtggtgaagc agaacaagcg
    35281 cgtgttgcag tacgtaatgt gcgtcgtgac gcgaacgaca aagtgaaagc actgttgaaa
    35341 gataaagaga tcagcgaaga cgacgatcgc cgttctcagg acgatgtaca gaaactgact
    35401 gatgctgcaa tcaagaaaat tgaagcggcg ctggcagaca aagaagcaga actgatgcag
    35461 ttctgatttc ttgaacgaca aaaacgccgc tcagtagatc cttgcggatc ggctggcggc
    35521 gttttgcttt ttattctgtc tcaactctgg atgtttcatg aagcaactca ccattctggg
    35581 ctcgaccggc tcgattggtt gcagcacgct ggacgtggtg cgccataatc ccgaacactt
    35641 ccgcgtagtt gcgctggtgg caggcaaaaa tgtcactcgc atggtagaac agtgcctgga
    35701 attctctccc cgctatgccg taatggacga tgaagcgagt gcgaaacttc ttaaaacgat
    35761 gctacagcaa caggggagcc gcaccgaagt cttaagtggg caacaagccg cttgcgatat
    35821 ggcagcgctt gaggaggttg atcaggtgat ggcagccatt gttggtgctg ctgggctgtt
    35881 acctacgctt gctgcgatcc gcgcgggtaa aaccattttg ctggccaata aagaatcact
    35941 ggttacctgc ggacgtctgt ttatggacgc cgtaaagcag agcaaagcgc aattgttacc
    36001 ggtcgatagc gaacataacg ctatttttca gagtttaccg caacctatcc agcataatct
    36061 gggatacgct gaccttgagc aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg
    36121 ccctttccgt gagacgccat tgcgcgattt ggcaacaatg acgcccgatc aagcttgccg
    36181 tcatccgaac tggtcgatgg gacgtaaaat ttctgtcgat tcggctacca tgatgaacaa
    36241 aggtctggaa tacattgaag cgcgttggct gtttaacgcc agcgccagcc agatggaagt
    36301 gctgattcac ccgcagtcag tgattcactc aatggtgcgc tatcaggacg gcagtgttct
    36361 ggcgcagctg ggggaaccgg atatgcgtac gccaattgcc cacaccatgg catggccgaa
    36421 tcgcgtgaac tctggcgtga agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc
    36481 cgcaccggat tatgagcgtt atccatgcct gaaactggcg atggaggcgt tcgaacaagg
    36541 ccaggcagcg acgacagcat tgaatgccgc aaacgaaatc accgttgctg cttttcttgc
    36601 gcaacaaatc cgctttacgg atatcgctgc gttgaattta tccgtactgg aaaaaatgga
    36661 tatgcgcgaa ccacaatgtg tggacgatgt gttatctgtt gatgcgaacg cgagtgaagt
    36721 cgccagaaaa gaggtgatgc gtctcgcaag ctgaggataa tccggctaca gagagtcgcg
    36781 ctatttgtta gcgtagggct tcagtgatat agtctgcgcc atctgatcgt aagtagttgg
    36841 ctttataagg tcaggtatgc cgtggtttta cacggctttt ttttgtatag gcttcagtat
    36901 tcctgagtac cgtaaaccct gtcagggaat aaaaaacgcg tgatgttgtc tgctactcaa
    36961 ccacttagcg aaaaattgcc agcgcatggc tgccgtcatg ttgcgatcat tatggacggc
    37021 aatggtcgct gggcaaaaaa gcaagggaag attcgtgcct ttgggcataa agccggggca
    37081 aaatccgtcc gccgggctgt ctcttttgcg gccaacaacg gtattgaggc gttaacgctg
    37141 tatgccttta gtagtgaaaa ctggaaccga ccagcgcagg aagtcagtgc gttaatggaa
    37201 ctgtttgtgt gggcgctcga tagcgaagta aaaagtctgc accgacataa cgtgcgtctg
    37261 cgtattattg gcgataccag tcgttttaac tcgcgtttgc aagaacgtat tcgtaaatct
    37321 gaagcgctaa cagccggaaa taccggtctg acgctgaata ttgcggcgaa ctacggtgga
    37381 cgttgggata tagtccaggg agtcaggcaa ctggctgaaa aggtgcagca aggaaacctg
    37441 caaccagatc agatagatga agagatgtta aaccagcatg tctgtatgca tgaactggcc
    37501 cctgtagatt tagtaattag gactggggga gagcatcgca ttagtaactt tttgctttgg
    37561 caaattgcct atgccgaact ttactttaca gatgttctct ggcccgattt cgatgaacaa
    37621 gactttgaag gggcgttaaa tgcctttgct aatcgagagc gtcgtttcgg cggcaccgag
    37681 cccggtgatg aaacagcctg atgggggtcg cttttgctga agtatcgcct gatatctgct
    37741 tttgtgttaa tacccgtcgt catcgcggcg ttgtttctgt tgccgccggt ggggttcgcc
    37801 attgtaacgc tggtggtctg catgctggca gcgtgggaat ggggacagct tagcggtttt
    37861 accactcgtt cgcagcgagt atggttggcg gtgttatgcg ggttattgtt ggcgctgatg
    37921 ctttttctgt tgccggaata tcaccgaaat attcatcaac cgctggttga aatctcactt
    37981 tgggcttcgt tgggttggtg gattgtcgcg ctattgctgg tgctgtttta cccaggttcc
    38041 gcagcaatct ggcgtaactc taaaacattg cgccttattt ttggcgtgct aaccattgtt
    38101 cccttcttct ggggcatgct ggcgttacgg gcctggcact atgacgagaa tcattacagt
    38161 ggcgcaatat ggctgctcta tgtcatgatc ctggtatggg gcgctgactc cggcgcatat
    38221 atgtttggca aattgtttgg taaacataag ctggcaccga aggtttctcc gggtaaaacc
    38281 tggcaaggct ttataggtgg actcgctact gcagcggtaa tctcatgggg ttatggcatg
    38341 tgggcgaatc tcgacgtcgc tcccgtcacc ttactcattt gctctattgt cgcagcgtta
    38401 gcctcagtgc tcggcgatct gaccgagagt atgtttaagc gtgaagcagg aattaaggac
    38461 agcggtcatt taattccagg acacggtggt attttagatc gtattgatag cctgacggct
    38521 gcggtaccgg tctttgcttg cttgttgtta ctggtattca ggacgcttta acggaaggta
    38581 atatgctgag ttttctctgg gatttggctt cgttcatcgt tgcactgggt gtacttatca
    38641 ccgtgcatga atttggtcat ttctgggttg cccggcgttg tggtgttcgc gttgagcgtt
    38701 tctcaatagg gtttggtaag gcgctctggc ggcgaactga taagctcggc accgaatatg
    38761 ttatcgccct gatcccgttg ggcggttatg tcaaaatgct ggatgagcgc gcagaaccgg
    38821 tcgttccgga actccgccac catgccttca ataataaatc tgtcggccaa cgagcggcga
    38881 ttattgccgc aggtccggtt gcaaacttca tttttgctat ctttgcctac tggctggttt
    38941 ttattattgg tgtgcctggc gtacgtccgg tggttggcga aatagcagcc aattcgatag
    39001 ctgcggaagc acaaattgca ccaggtacgg aactaaaagc cgtagatggt atcgaaacgc
    39061 ctgattggga tgccgtgcgt ttgcagttgg tcgataaaat tggcgatgaa agcaccacca
    39121 ttacagtagc gccatttgac agcgaccaac ggcgggatgt aaagctcgat ttacgtcact
    39181 gggcgtttga gcctgataaa gaagatccgg tatcttcgct ggggattcgt cctcgtgggc
    39241 cgcaaattga acctgtactg gaaaatgtgc agccaaactc ggcggcaagc aaggcaggtt
    39301 tgcaagcagg cgacaggatc gttaaagtcg atggtcagcc cttaacgcag tgggtgacct
    39361 ttgtgatgct tgtccgggat aacccgggta aatccttagc gttagaaatc gaaaggcagg
    39421 ggagtccctt gtctttgaca ttaatcccgg agagtaaacc gggtaatggt aaagcgattg
    39481 gttttgtcgg tattgagccg aaagtcattc ctttgccaga tgagtataaa gttgtacgcc
    39541 agtatgggcc gttcaacgcc atcgtcgaag ccacggacaa aacgtggcag ctgatgaagc
    39601 tgacggtcag tatgctggga aaattgatca ccggtgatgt gaaactgaac aacctcagtg
    39661 ggccgatctc tatcgccaag ggggctggga tgacagcgga actcggggta gtttattacc
    39721 tgccgtttct tgcgcttatt agcgtgaact tagggataat taacctgttt ccgttgcccg
    39781 tacttgacgg ggggcatctg ctgttccttg cgatcgaaaa gatcaagggc ggaccggtat
    39841 ccgagcgggt tcaagacttt tgttatcgca ttggctcgat tctgctggtg ctgttaatgg
    39901 ggcttgcact tttcaatgat ttctctcggt tatgagagtt agttaggaag aacgcataat
    39961 aacgatggcg atgaaaaagt tgctcatagc gtcgctgctg tttagcagcg ccaccgtata
    40021 cggtgctgaa gggttcgtag tgaaagatat tcatttcgaa ggccttcagc gtgtcgccgt
    40081 tggtgcggcc ctcctcagta tgccggtgcg tacaggcgac acggttaatg atgaagatat
    40141 cagtaatacc attcgcgctc tgtttgctac cggcaacttt gaggatgttc gcgtccttcg
    40201 tgatggtgat acccttctgg ttcaggtaaa agaacgtccg accattgcca gcattacttt
    40261 ctccggtaac aaatcggtga aagatgacat gctgaagcaa aacctcgagg cttctggtgt
    40321 gcgtgtgggc gaatccctcg atcgcaccac cattgccgat atcgagaaag gtctggaaga
    40381 cttctactac agcgtcggta aatatagcgc cagcgttaaa gctgtcgtga ccccgctgcc
    40441 gcgcaaccgt gttgacctaa aactggtgtt ccaggaaggt gtgtcagctg aaatccagca
    40501 aattaacatt gttggtaacc atgctttcac caccgacgaa ctgatctctc atttccaact
    40561 gcgtgacgaa gtgccgtggt ggaatgtggt aggcgatcgt aaataccaga aacagaaact
    40621 ggcgggcgat cttgaaaccc tgcgcagcta ctatctggac cgcggttatg cccgtttcaa
    40681 catcgactct acccaggtca gtctgacgcc agataaaaaa ggtatttacg tcacggtgaa
    40741 catcaccgaa ggcgatcagt acaagctttc tggcgttgaa gtgagcggca accttgccgg
    40801 gcactccgct gaaattgagc aactgactaa gatcgagccg ggcgagctgt ataacggcac
    40861 taaagtgacc aaaatggaag atgacattaa aaagcttctc ggtcgctatg gttatgccta
    40921 tccgcgcgta cagtcgatgc ctgaaattaa cgatgccgac aaaaccgtta aattacgcgt
    40981 gaacgttgat gcgggtaacc gtttctacgt gcgtaagatc cgttttgaag gtaacgatac
    41041 ctcgaaagat gccgtcctgc gtcgcgaaat gcgtcagatg gaaggtgcat ggttggggag
    41101 cgatctggtc gatcagggta aggagcgtct gaatcgtctg ggcttctttg aaactgtcga
    41161 taccgatacc caacgtgttc cgggtagccc ggaccaggtt gatgtagtct acaaggtgaa
    41221 agaacgtaac actggtagct tcaacttcgg gattggttac ggtactgaaa gtggcgtgag
    41281 cttccaggct ggcgtgcaac aggataactg gttaggtaca ggttatgctg ttggtatcaa
    41341 cgggaccaaa aacgattacc agacctatgc tgaactgtcg gtaaccaacc cgtacttcac
    41401 cgtagatggc gtaagcctcg gtggtcgtct cttctataat gacttccagg cagatgacgc
    41461 cgacctgtcc gactatacca acaagagtta tggtacagac gtgacgttgg gcttcccgat
    41521 taacgaatat aactcgctgc gtgcaggtct gggttatgta cataactccc tgtccaacat
    41581 gcagcctcag gttgcgatgt ggcgttatct gtactctatg ggtgaacatc cgagcacctc
    41641 tgatcaggat aacagcttca aaacggacga cttcacgttc aactatggtt ggacctataa
    41701 caagcttgac cgtggttact tcccgacaga tggttcacgt gtcaacctga ccggtaaagt
    41761 gaccattcct ggatcggata acgaatacta caaagtgacg ttagacacgg cgacttatgt
    41821 gccgatcgat gacgatcaca aatgggttgt tctgggtcgt acccgctggg gttatggtga
    41881 tggtttaggc ggcaaagaga tgccgttcta cgagaacttc tatgccggtg gttccagcac
    41941 cgtgcgtggc ttccagtcca ataccattgg tccgaaagca gtttacttcc cgcatcaggc
    42001 cagtaattat gatccggact atgattacga atgtgcgact caggacggcg cgaaagacct
    42061 gtgtaaatcg gatgatgctg taggcggtaa cgccatggcg gttgccagcc tcgagttcat
    42121 caccccgacg ccgtttatta gcgataagta tgctaactcg gttcgtactt cattcttctg
    42181 ggatatgggt accgtttggg atacaaactg ggattccagc caatattctg gatatccgga
    42241 ctatagtgat ccaagcaata tccgtatgtc tgcgggtatc gcattacaat ggatgtcccc
    42301 attggggccg ttggtgttct cctacgccca gccgttcaaa aagtacgatg gagacaaggc
    42361 agaacagttc cagtttaaca tcggtaaaac ctggtaagtg ttctccacaa aggaatgtag
    42421 tggtagtgta gcgatgactt taggcgatca atataagatc gccgggccac gcaaagaact
    42481 gcaccctccg gtgcaaatgg gatggtaagg agtttattgt gaaaaagtgg ttattagctg
    42541 caggtcttgg tttagcactg gcaacttctg ctcaggcggc tgacaaaatt gcaatcgtca
    42601 atatgggcag cctgttccag caggtagcgc agaaaaccgg tgtttctaac acgctggaaa
    42661 atgagttcaa aggccgtgcc agcgaactgc agcgtatgga aaccgatctg caggctaaaa
    42721 tgaaaaaact gcagtccatg aaagcgggca gcgatcgcac caaactggaa aaagacgtga
    42781 tggctcagcg ccagactttt gctcagaaag cgcaggcttt tgagcaggat cgcgcacgtc
    42841 gttccaacga agaacgcggc aaactggtta ctcgtatcca gactgctgtg aaatccgttg
    42901 ccaacagcca ggatatcgat ctggttgttg atgcaaacgc cgttgcttac aacagcagcg
    42961 atgtaaaaga catcactgcc gacgtactga aacaggttaa ataagtaatg ccttcaattc
    43021 gactggctga tttagcgcag cagttggatg cagaactaca cggtgatggc gatatcgtca
    43081 tcaccggcgt tgcgtccatg caatctgcac aaacaggtca cattacgttc atggttaacc
    43141 caaaataccg tgagcattta ggcttgtgcc aggcgtccgc ggttgtcatg acccaggacg
    43201 atcttccttt cgcgaaaagt gccgcgctgg tagtgaagaa tccctacctg acttacgcgc
    43261 gcatggcgca aattttagat accacgccgc agcccgcgca gaacattgca cccagtgcgg
    43321 tgatcgacgc gacggcgaag ctgggtaaca acgtatcgat tggcgctaac gcggtgattg
    43381 agtccggcgt tgaactgggc gataacgtga ttatcggtgc cggttgcttc gtaggtaaaa
    43441 acagcaaaat cggtgcaggt tcgcgtctct gggcgaacgt aaccatttac catgagatcc
    43501 agatcggtca gaattgcctg atccagtccg gaacagtggt aggcgcagac ggctttggtt
    43561 atgccaacga tcgtggtaac tgggtgaaga tcccacagat tggtcgcgta attattggcg
    43621 atcgcgtgga gatcggtgcc tgcacaacca tcgatcgcgg cgcgctggat gacactgtta
    43681 ttggcaatgg cgtgatcatt gataaccagt gccagattgc acataacgtc gtgattggcg
    43741 acaatacggc ggttgccggt ggcgtcatta tggcgggcag cctgaaaatt ggtcgttact
    43801 gcatgatcgg cggagccagc gtaatcaacg ggcatatgga aatatgcgac aaagtgacgg
    43861 ttacgggcat gggtatggtg atgcgtccca tcactgaacc aggcgtctat tcctcaggca
    43921 ttccgctgca acccaacaaa gtctggcgca aaaccgctgc actggtgatg aacattgatg
    43981 acatgagcaa gcgtctgaaa tcgcttgagc gcaaggttaa tcaacaagac taacgttcca
    44041 tcttttgttc gccaaacttt acggcctgtc tcattcttac gattgcggca ggccgtgtta
    44101 ttattgtcgt ttcttatatt ttgacaggaa gagtatcttg actactaaca ctcatactct
    44161 gcagattgaa gagattttag aacttctgcc gcaccgtttc ccgttcttac tggtggatcg
    44221 cgtgctggat tttgaagaag gtcgttttct gcgcgcagta aaaaatgtct ctgtcaatga
    44281 gccattcttc cagggccatt tccctggaaa accgattttc ccgggtgtgc tgattctgga
    44341 agcaatggca caggcaacag gtattctggc gtttaaaagc gtaggaaaac tggaaccggg
    44401 tgagctgtac tacttcgctg gtattgacga agcgcgcttc aagcgcccgg tcgtgcctgg
    44461 cgatcaaatg atcatggaag tcactttcga aaaaacgcgc cgcggcctga cccgttttaa
    44521 aggggttgct ctggtcgatg gtaaagtagt ttgcgaagca acgatgatgt gtgctcgtag
    44581 ccgggaggcc tgatacgtga ttgataaatc cgcctttgtg catccaaccg ccattgtgga
    44641 agagggcgcg tcaattggcg cgaacgcaca cattggtcct ttttgtatcg ttggacccca
    44701 tgtcgaaatt ggtgagggta ccgtactgaa atctcacgtt gtcgtgaatg gtcatactaa
    44761 aattggccgc gataatgaga tttatcagtt tgcctccatc ggcgaagtta accaggattt
    44821 gaaatatgct ggcgaaccga cccgtgtgga aatcggcgat cgtaaccgca ttcgcgaaag
    44881 cgtcaccatt catcgtggca cagtccaggg cggtggattg acgaaggtgg gcagcgacaa
    44941 cttactgatg atcaacgcgc acattgcgca cgattgtacg gtaggtaacc gctgtattct
    45001 cgccaacaac gcaacgctgg cgggtcacgt atcggttgac gacttcgcga tcatcggcgg
    45061 catgaccgca gtccatcagt tctgcatcat tggtgcgcat gtgatggttg gcggctgctc
    45121 cggtgtggcg caggacgtcc ctccttatgt tattgcgcag ggtaaccacg caacgccgtt
    45181 cggtgtcaat atcgaagggc tgaagcgccg cggattcagc cgtgaggcga ttaccgctat
    45241 ccgcaatgcg tataagctaa tttatcgtag cggtaaaacg ctcgatgaag tgaaaccgga
    45301 aattgctgaa ctggcggaaa catatccgga agtgaaagcc tttaccgatt tctttgcacg
    45361 ctcaacgcgc ggtctgattc gttaatgact gaccagcgtc cattaacgat tgccctggtc
    45421 gccggagaaa cctccggcga tatcctgggg gccggtttaa tccgcgctct gaaagaacgt
    45481 gtgcccaacg cccgctttgt tggtgttgcc gggccacgaa tgcaggctga aggctgcgaa
    45541 gcctggtacg aaatggaaga actggcggtg atgggcattg ttgaagtgct cggtcgtctg
    45601 cgtcgcttac tgcatattcg tgccgatctg acaaagcgtt ttggcgaact gaagccagat
    45661 gtttttgttg gtattgatgc gcccgacttc aatattactc ttgaaggtaa cctcaaaaag
    45721 cagggtatca aaaccattca ttacgtcagt ccatccgtct gggcgtggcg acagaaacgc
    45781 gttttcaaaa taggcagagc caccgatctg gtgctcgcat ttctgccttt cgaaaaagcg
    45841 ttttatgaca aatacaatgt accgtgccgc tttatcggtc ataccatggc tgatgccatg
    45901 ccgttagatc cagataaaaa tggtgcccgt gatgtgctgg ggatccctta cgatgcccac
    45961 tgtctggcat tgttgccggg cagccgtggt gcagaagtcg aaatgcttag tgccgatttc
    46021 ttgaaaactg cccagctttt gcgccagaca tatccggatc tcgaaatcgt ggtgccgctg
    46081 gtgaatgcca aacgccgcga gcagtttgaa cgcatcaaag ctgaagtcgc gccagacctg
    46141 tcggttcatt tgctggatgg aatgggccgt gaggcgatgg tcgccagcga tgcggcacta
    46201 ctggcgtcgg ggacggcagc cctggagtgt atgctggcga aatgcccgat ggtggtggga
    46261 tatcgcatga agccttttac cttctggttg gcgaagcggc tggtgaaaac tgattatgtc
    46321 tcgctgccaa atctgctggc gggcagagag ttagtcaaag agttattgca ggaagagtgt
    46381 gagccgcaaa aactggctgc ggcgctgtta ccgctgttgg cgaacgggaa aaccagccac
    46441 gcgatgcacg ataccttccg tgaactgcat cagcagatcc gctgcaatgc cgatgagcag
    46501 gcggcacaag ccgttctgga gttagcacaa tgatcgaatt tgtttatccg cacacgcagc
    46561 tggttgcggg tgtggatgaa gtcggacgcg ggccgttagt tggcgcggtc gtcaccgctg
    46621 cggtgatcct tgacccggcg cgcccgattg ccgggctgaa tgattccaaa aagctgagcg
    46681 aaaaacgccg tctggcgctc tatgaagaga tcaaagagaa agcgttgagc tggagtctgg
    46741 gccgcgcgga accccacgaa atcgacgagc tgaacattct tcatgcgacc atgctggcga
    46801 tgcagcgtgc cgtcgctggg ctgcatattg cgccggaata tgtgttgatt gatggtaacc
    46861 gctgcccgaa attaccgatg cctgcgatgg ctgtggtgaa aggggatagc cgcgtaccag
    46921 aaatcagtgc cgcgtctatc ctggcgaaag tgacgcgtga cgccgaaatg gcggcgctgg
    46981 atattgtttt cccgcaatat ggttttgccc aacataaagg gtatccaacc gcttttcatc
    47041 tggaaaaact ggctgaatat ggcgcgactg aacatcatcg tcgcagcttt gggcctgtca
    47101 aacgcgcact gggacttgcg tcctgattct tgtgtcgaga ttaagtaaac cggaatctga
    47161 agatgtctga accacgtttc gtacacctgc gggtgcacag cgactactcg atgatcgatg
    47221 gcttggccaa aaccgcaccg ctggtaaaaa aggcggcggc gttgggtatg ccagcactgg
    47281 cgatcaccga tttcaccaac ctttgcggtc tggtgaagtt ctacggagcg ggacatggcg
    47341 cagggattaa gcctatcgtc ggggcagatt ttaatgtcca gtgcgacctg ctgggtgatg
    47401 agttaaccca cctgacggta ctggcggcga acaataccgg ctatcagaat ctggcgttgc
    47461 tgatctcaaa agcgtatcag cgcgggtacg gtgccgccgg gccgatcatc gatcgcgact
    47521 ggcttatcga attaaacgaa gggttgatcc ttctttccgg cgggcgcatg ggcgacgtcg
    47581 gacgcagtct tttgcgtggt aacagcgcgc tggtagatga gtgtgtcgcg ttttatgaag
    47641 aacacttccc ggatcgctat tttctcgagc tgatccgcac cggcaggccg gatgaagaaa
    47701 gctatctgca cgcggcggtg gaactggcgg aagcgcgcgg tttgcccgtc gtggcgacta
    47761 acgatgtgcg ctttatcgac agcagcgact ttgacgcaca cgaaatccgc gtcgcgatcc
    47821 acgacggctt taccctcgac gatcctaaac gcccgcgtaa ctattcgccg cagcaatata
    47881 tgcgtagcga agaggagatg tgtgagctgt ttgccgacat cccagaagcc cttgccaaca
    47941 ccgttgagat cgccaaacgc tgtaacgtaa ctgtgcgtct tggtgaatac ttcctgccgc
    48001 agttcccgac cggggacatg agcaccgaag attatctggt caagcgtgca aaagaaggcc
    48061 tggaagagcg tctggcattt ttattccctg acgaggaaga acgtcttaaa cgccgcccgg
    48121 aatatgacga acgtctggag actgaacttc aggttatcaa ccagatgggc ttcccgggct
    48181 acttcctcat cgttatggaa tttatccagt ggtcgaaaga taacggcgta ccggtagggc
    48241 caggccgtgg ctccggtgcg ggttcactgg tggcctacgc gctgaaaatc accgacctcg
    48301 atccgctgga atttgacctg ctgttcgaac gtttccttaa cccggaacgt gtctccatgc
    48361 ctgacttcga cgttgacttc tgtatggaga aacgcgatca ggttatcgaa cacgtggcgg
    48421 acatgtacgg tcgtgatgcg gtatcgcaga ttatcacctt cggtacgatg gcggcgaaag
    48481 cggtgatccg cgacgtaggc cgcgtgctgg ggcatccgta cggctttgtc gaccgtatct
    48541 cgaaactgat cccgcccgat ccggggatga cgctggcgaa agcgtttgaa gccgagccgc
    48601 agctgccgga aatctacgaa gcggatgaag aagttaaggc gctgatcgac atggcgcgca
    48661 aactggaagg ggtcacccgt aacgccggta agcacgccgg tggggtggtt atcgcgccga
    48721 ccaaaattac cgattttgcg ccgctttatt gcgatgaaga gggcaaacat ccggtcaccc
    48781 agtttgataa aagcgacgtt gaatacgccg gactggtgaa gttcgacttc cttggtttgc
    48841 gtacgctcac catcatcaac tgggcgctgg agatgatcaa caagcggcgg gcgaagaatg
    48901 gcgagccacc gctggatatc gccgcgatcc cgctggatga caagaaaagc ttcgacatgc
    48961 tgcaacgctc ggaaaccacg gcggtattcc agcttgaatc gcgcggcatg aaggacctga
    49021 tcaagcgtct gcaacctgac tgcttcgaag atatgatcgc actggtggca ctgttccgcc
    49081 ctggtccgtt gcagtcaggg atggtggata actttatcga ccgtaaacat ggtcgtgaag
    49141 agatctccta tccggacgtg cagtggcagc atgaaagcct gaaatcggta ctggagccaa
    49201 cctacggcat tatcttgtat caggaacagg tcatgcagat tgcccaggtg ctttctggtt
    49261 ataccctcgg tggcgcggat atgctgcgcc gtgcgatggg taagaaaaag ccggaagaga
    49321 tggctaagca gcgttctgta tttgctgaag gtgcagaaaa gaacggaatc aacgccgaac
    49381 tggcgatgaa aatcttcgac ctggtggaga aattcgcggg ttacggattt aacaaatcgc
    49441 actctgcggc ctatgctttg gtgtcatatc aaacgttatg gctgaaagcg cactatccgg
    49501 cggagtttat ggcggcggta atgaccgccg atatggacaa caccgagaag gtggtgggcc
    49561 tggtggatga gtgctggcgg atggggctga aaatcctgcc accagatata aactccggtc
    49621 tttaccattt ccacgtcaac gacgatggcg aaatcgtgta tggtattggc gccatcaaag
    49681 gggtaggtga aggtccgatt gaggccatca tcgaagcccg taataaaggc ggctacttcc
    49741 gcgaactgtt tgatctctgc gcccgtaccg acaccaaaaa gttaaaccgg cgggtgctgg
    49801 aaaaactgat catgtccggg gcgtttgacc gtcttgggcc acaccgcgcg gcgctgatga
    49861 actcgctggg tgatgcgtta aaagcggcag atcaacacgc gaaagcggaa gctatcggtc
    49921 aggccgatat gttcggcgtg ctggccgaag agccggaaca aattgaacaa tcctacgcca
    49981 gctgccaacc gtggccggag caggtggtat tagatgggga acgtgaaacg ttaggcctgt
    50041 acctgaccgg acaccctatc aaccagtatt taaaagagat tgagcgttat gtcggaggcg
    50101 taaggctgaa agacatgcac ccgacagaac gtggtaaagt catcacggct gcggggctcg
    50161 ttgttgccgc gcgggttatg gtcaccaagc gcggcaatcg tatcggtatc tgcacgctgg
    50221 atgaccgttc cgggcggctg gaagtgatgt tgtttactga cgccctggat aaataccagc
    50281 aattgctgga aaaagaccgc atacttatcg tcagcggaca ggtcagcttt gatgacttca
    50341 gcggtgggct taaaatgacc gctcgcgaag tgatggatat tgacgaagcc cgggaaaaat
    50401 atgctcgcgg gcttgctatc tcgctgacgg acaggcaaat tgatgaccag cttttaaacc
    50461 gactccgtca gtctctggaa ccccaccgct ctgggacaat tccagtacat ctctactatc
    50521 agagggcgga tgcacgcgcg cggttgcgtt ttggcgcgac gtggcgtgtc tctccgagcg
    50581 atcgtttatt aaacgatctc cgtggcctca ttggttcgga gcaggtggaa ctggagtttg
    50641 actaatacag gaatactatg agtctgaatt tccttgattt tgaacagccg attgcagagc
    50701 tggaagcgaa aatcgattct ctgactgcgg ttagccgtca ggatgagaaa ctggatatta
    50761 acatcgatga agaagtgcat cgtctgcgtg aaaaaagcgt agaactgacg cgtaaaatct
    50821 tcgccgatct cggtgcatgg cagattgcgc aactggcacg ccatccgcag cgtccttata
    50881 ccctggatta cgttcgcctg gcatttgatg aatttgacga actggctggc gaccgtgcgt
    50941 atgcagacga taaagctatc gtcggtggta tcgcccgtct cgatggtcgt ccggtgatga
    51001 tcattggtca tcaaaaaggt cgtgaaacca aagaaaaaat tcgccgtaac tttggtatgc
    51061 cagcgccaga aggttaccgc aaagcactgc gtctgatgca aatggctgaa cgctttaaga
    51121 tgcctatcat cacctttatc gacaccccgg gggcttatcc tggcgtgggc gcagaagagc
    51181 gtggtcagtc tgaagccatt gcacgcaacc tgcgtgaaat gtctcgcctt ggcgtaccgg
    51241 tagtttgtac ggttatcggt gaaggtggtt ctggcggtgc gctggcgatt ggcgtgggcg
    51301 ataaagtgaa tatgctgcaa tacagcacct attccgttat ctcgccggaa ggttgtgcgt
    51361 ccattctgtg gaagagcgcc gacaaagcgc cgctggcggc tgaagcgatg ggtatcatcg
    51421 ctccgcgtct gaaagagctg aaactgatcg actccatcat cccggaaccg ctgggcggcg
    51481 ctcaccgtaa cccggaagcg atggcggcat cgttgaaagc gcaactgctg gcggatctgg
    51541 ccgatctcga tgtgttaagc actgaagatt taaaaaatcg tcgttatcag cgcctgatga
    51601 gctacggtta cgcgtaattc gcaaaagttc tgaaaaaggg tcacttcggt ggcccttttt
    51661 tatcgccacg gtttgagcag gctatgatta aggaaggatt ttccaggagg aacacatgaa
    51721 catcattgcc attatgggac cgcatggcgt cttttataaa gatgagccca tcaaagaact
    51781 ggagtcggcg ctggtggcgc aaggctttca gattatctgg ccacaaaaca gcgttgattt
    51841 gctgaaattt atcgaacata accctcgaat ttgcggcgtg atttttgact gggatgagta
    51901 cagtctcgat ttatgcagcg atatcaatca acttaatgaa tatctcccgc tttatgcctt
    51961 catcaacacc cactcgacga tggatgtcag cgtgcaggat atgcggatgg cgctctggtt
    52021 ttttgaatat gcgctggggc aggcggaaga tatcgccatt cgtatgcgtc agtacaccaa
    52081 cgaatatctt gataacatta cgccgccgtt cacgaaagcc ttgtttacct acgtcaaaga
    52141 gcggaagtac accttttgta cgccggggca tatgggcggc accgcatatc aaaaaagccc
    52201 ggttggctgt ctgttttatg attttttcgg cgggaatacc cttaaggctg atgtctctat
    52261 ttcggtcacc gagcttggtt cgttgcttga ccacaccggg ccacacctgg aagcggaaga
    52321 gtacatcgcg cggacttttg gcgcggaaca gagttatatc gttaccaacg gaatatcgac
    52381 gtcgaacaaa attgtgggta tgtacgccgc gccatccggc agtacgctgt tgatcgaccg
    52441 caactgccat aaatcactgg cgcatctgtt gatgatgaac gatgtagtgc cagtctggct
    52501 gaaaccgacg cgtaatgcgt tggggattct gggggggatc ccgcgccgtg aatttactcg
    52561 cgacagcatc gaagagaaag tcgctgccac tacgcaagca caatggccgg ttcatgcggt
    52621 gatcaccaac tccacctatg atggtttgct ctacaacacc gactggatca aacagacgct
    52681 ggatgtcccg tcgatccact tcgattccgc ctgggtgccg tatactcatt ttcatccaat
    52741 ctaccagggt aaaagtggta tgagcggcga gcgtgttgcg ggaaaagttt tcttcgaaac
    52801 gcaatcgacc cacaaaatgc tggcggcgtt atcgcaggct tcactgatcc acattaaagg
    52861 cgagtatgac gaagaggcgt ttaacgaagc ctttatgatg cataccacca cctcgcccag
    52921 ttatcccatt gttgcttcgg ttaagacggc ggcggcgatg ctgcgtggta atccgggcaa
    52981 acggctgatt aaccgttcgg tggagcgggc gctgcatttt cgcaaagagg tccagcggct
    53041 gcgggaagag tctgacggtt ggtttttcga tatctggcaa ccgccgcagg tggatgaagc
    53101 cgaatgcagg cccgttgcgc ctggcgaaca atggcacggc tttaacgatg cggatgccga
    53161 tcatatgttt ctcgatccgg ttaaagtcac tattttgaca ccggggatgg acgaacaggg
    53221 caatatgagt gaggagggga tcccggcggc gctggtagca aaattcctcg acgaacgtgg
    53281 gatcgtagta gagaaaaccg gcccttataa cctgctgttt ctctttagta ttggcatcga
    53341 taaaaccaaa gcaatgggat tattgcgtgg gttgacggaa tttaagcgct cttacgatct
    53401 caacctgcgg atcaaaaata tgctgcccga tctctatgct gaagatcccg atttctaccg
    53461 caatatgcgt attcaggatc tggcgcaagg gatccataag ctgattcgta aacacgatct
    53521 ttccggtttg atgttgcggg cattcgatac tttgccggaa atgatcatga cgccacatca
    53581 ggcatggcag agacagatta aaggtgaagt agaaaccatt gcgctggaac aactggtcgg
    53641 tagagtatcg gcaaatatga tcctgcctta tccaccgggc gtaccgctgt tgatgccagg
    53701 agaaatgctg accaaagaga gccgcacggt actcgatttt ctactgatgc tttgttccgt
    53761 agggcaacat taccccggtt ttgaaacgga tattcacggc gcgaaacagg acgaagacgg
    53821 cgtttaccgc gtacgagtcc taaaaatggc gggataactt gccagagcgg cttccgggcg
    53881 agtaacgtgc tgttaacaaa taaaggagac gttatgctgg gtttaaaaca ggttcaccat
    53941 attgcgatta ttgcgacgga ttatgcggtg agcaaagctt tctactgcga tattcttggt
    54001 ttcacgctgc aaagcgaagt ctatcgcgaa gcgcgcgact cgtggaaagg ggatttggcg
    54061 cttaatgggc aatatgtgat tgagcttttc tcttttccgt tcccgccgga gcgccccagc
    54121 cgaccggaag cttgcggcct gcgtcatctg gcttttagcg ttgatgacat cgatgcggca
    54181 gtggcgcacc ttgaaagcca taacgtgaag tgtgaagcca tccgtgtcga tccatacacg
    54241 caaaaacgct tcaccttctt taacgatccg gacgggctgc cgttggaact gtatgagcag
    54301 taaggcttgt catcgccgca tttgcccggt aacgtgccgg gcattgctac tgtaaaatcg
    54361 caccatcatg acactcacgc tcaatagaca acttctcacc tcacgccaga ttctggtggc
    54421 ctttagcggc gggcttgact ccaccgttct gctgcatcag ttggtgcagt ggcggacgga
    54481 aaatccgggc gtcactctgc gcgctattca tgtgcatcac ggtttaagtg ccaatgccga
    54541 tgcctgggtt acgcattgcg aaaacgtctg ccaacagtgg caggtgccgc tggtggtcga
    54601 acgcgtacaa cttgcgcaag aggggctggg gattgaggcc caggcgcggc aggcacgtta
    54661 tcaggcattt gcccgtaccc tattgcccgg cgaagtgctg gtcaccgcgc aacatctcga
    54721 cgatcaatgt gaaacctttc tgctggcgct aaaacgcggt agcggccctg ccgggctttc
    54781 ggctatggcg gaagtttcgg agtttgctgg aacacggctt atccgcccgt tgcttgcccg
    54841 cacgcggggg gaactggagc agtgggcatt ggcgcatggt ttacgctgga ttgaagacga
    54901 aagtaatcag gacgactcat acgatcgtaa ctttctgcgc ctgcgcgtag tgccgttatt
    54961 gcagcagcgt tggccgcatt ttgcggaagc aacggcccgc agcgccacac tttgtgctga
    55021 acaagagagt ctgttggatg aactgctggc ggatgattta gcacactgcc aaacgtcgca
    55081 ggggacgctg cagattgcgc caatgctggc gatgagtgat gcccgtcgcg cggcgattat
    55141 tcgccgctgg ctggcagggc agaatgcacc gatgccttcc cgcgacgcgc tggtgcgcat
    55201 ctggcaggaa gtggcgctgg cgcgggaaga tgcttccccc tgtttacgtt tgggcgcctt
    55261 tgaaatccgc cgctatcagt cgcaactgtg gtggattaaa tccgtcaccg ggcaaagcga
    55321 aaccattgtg ctgtggcaga cgtggctaca accgctggaa ttaccggcgg ggctgggaac
    55381 cgtacagctt actgcgggag gcgatattcg ccctccgcgt gcagacgaag cggtcagcgt
    55441 gcgtttcaaa gcgccaggat tgctgcatat tgtcgggcgc aacggcggac gtaagctgaa
    55501 gaaaatctgg caagagttag gcgtgccgcc gtggctacgt gacaccacgc cactgctgtt
    55561 ttatggagaa acgctgattg cggcggcagg ggtatttgtg acgcaagaag gtgtggctga
    55621 aggtgagaat ggcgtaagtt ttgtctggca gaaaacgctt agttaagtga aagccggata
    55681 agacgcatca aacgtcgcat ccggcgaaag tcaatcagga ttcgcttacc accaccgtac
    55741 cgatttcagg gtggctaaag ctggtaattt tatccagacg cagctcgcgg gtttcgccag
    55801 cggcctcgac gaccagatac tccacatttt tgcgggagac taaatcactg gcttttgcct
    55861 gcaatttttc gccatctttc agctcaagtg tcagcattaa atgatgctgg caggcgagct
    55921 caagattatc gtaatcatca caattaattg gttgatacgt atcattcatt gacataatcg
    55981 ctcaccagta agtttgccgc agcgtatgct gctttttccc tgacagcctc agaaagggcg
    56041 tcgtcggcag ccatttcatt cagcactttc aaaacgcagc ccagcgcgtc cggaacgtat
    56101 cctaagtctc cgctggcgat ttccgcgtac cgcttgcgta ttaactcaca atatttttcc
    56161 acatgccctc ctgtcagcac tctgacttaa ccgtggatgc aagtctaagc ctacgaagat
    56221 aaactctgtt tcgcaaggtg actataccac actcatttct gcaatatcag cgccgcaact
    56281 gcacgtattc cgttacaatg gcctcctgat tcgaaaggag ttttcttatg gcgcttaaag
    56341 cgacaattta taaagcgacg gttaatgtgg ccgatctcga ccgcaaccag tttctcgatg
    56401 cctctctgac gctggcgcgc cacccttcag aaacccagga gcgtatgatg ctgcgcttgc
    56461 tggcgtggct gaaatatgct gatgaacgtc tgcaatttac ccgtggtttg tgtgccgatg
    56521 atgagccgga agcgtggctg cgtaacgatc acctgggcat tgatttgtgg attgagctgg
    56581 ggctgccgga tgagcgacga attaagaaag cctgcaccca ggccgcagaa gtggcgctgt
    56641 ttacctataa tagtcgggcg gcgcaaatct ggtggcagca aaatcagagc aaatgtgtgc
    56701 agtttgccaa tctttccgtc tggtatctgg acgatgaaca actggcgaaa gtaagcgcct
    56761 ttgccgatcg taccatgacg ctgcaggcaa cgattcagga tggtgttatc tggttatcgg
    56821 atgataagaa taatctggaa gttaacttaa ccgcctggca acaaccttca tgattgtgat
    56881 ttcccgacat gttgctattc ccgatggtga gcttgagatc accgccattc gtgcgcaggg
    56941 cgctggcggg cagcatgtta ataagacctc aacggctatt catctgcgtt ttgacattcg
    57001 ggcgtccagc ctgccagagt attacaaaga gcgtctgctc gccgccagcc atcatttgat
    57061 cagcagtgat ggcgtgattg tcattaaggc acaggaatac cgcagtcagg aactgaaccg
    57121 cgaagccgct ctggcccggc tggtggcagt gattaaagat ttaacaaaag aacaaaaagc
    57181 ccgacgaccc acgcggccca cccgtgcatc gaaagagcgc aggctggcat cgaaagcaca
    57241 aaaatcaagc gtgaaggcga tgcgcggcaa agtacgcagc ggtcgggaat aaaaagaagg
    57301 aatggatggt gaaaaaagcg atagtgacag cgatggctgt aatcagcctc tttactctga
    57361 tgggatgtaa taatcgggcc gaagtcgata cgctttctcc ggcgcaggct gccgaactga
    57421 aaccgatgcc gcaaagttgg cgcggcgtgc taccgtgtgc cgattgcgaa ggaatcgaaa
    57481 tctctctgtt cctcgaaaaa gacggaacat gggtgatgaa tgagcgttat ctcggtgctc
    57541 gtgaagaacc ttcctccttc gcttcctacg gtacatgggc gcgaaccgct gacaagctgg
    57601 tattaaccga cagcaaaggt gaaaagtcat attatcgggc gaaaggcgat gcgctggaga
    57661 tgctcgatcg tgaaggcaat ccaattgaat cgcagttcaa ctatacgctg gaaccggcac
    57721 aatccagttt acctatgacg ccgatgaccc tgcgaggcat gtatttttat atggctgatg
    57781 cggcgacctt cactgattgc gcgaccggaa aacgtttcat ggtagcgaat aacgcagagc
    57841 tggagcgtag ctacctggct gcgcgcggtc acagtgaaaa accggtgtta ctgtcagtag
    57901 aaggtcactt tacgcttgag gctaatccgg atactggtgc gccaaccaaa gtattagcgc
    57961 ccgatacggc gggtaaattt tacccgaata aggattgcag tagtttgggg cagtaaacct
    58021 gcttaaggca gaaacaaacg caaaattgcc tgatgcgctg cgcttatcag gcctacagca
    58081 tataatgcaa cattttgaat tagcacgatt ttgtaggccg gataaggcgt ttacgccgca
    58141 tccggcattt tgtgcacgag gctggcttta tcactgagtc agcccaacaa agcgacccgc
    58201 cttgatataa atcccaggct tcaggtgccc aacatactgt aactgcgttt ctggcttaaa
    58261 cgcagagaca tcaccaatac gcatatgcat cagatcggca gggctaatcc agcccgattg
    58321 cgccagtgta agtggatgcc cggatttggc aaatgcatca gtgacaaatt ccgaacaaaa
    58381 ccacgacttt ttgtctcctt cgcacacact gcttaactgc gctttcgcca ggtcgctgac
    58441 gcactgttgg cgaaaatcct cggagaacgg attcagtgag cacatctggc gagtcaccat
    58501 aaagggaata aattcgacaa tgccgcgata gttataaccg ctatctttga ttttattggc
    58561 aaacgcggtg atatctgtgg cttgttgcgg ggtaagatcc gggactcgta agacgaaaag
    58621 cttatcacta tgcttcatcg cttttttaag ggaaacaatc tggacgccag cgcctgtcgc
    58681 ttctgcaacg ttattatcac ccagaaagat tgcaacgtga ctcacagagg aagtgctgaa
    58741 gacgcggatt ccaaatgagg ttaccccaag gcttgaggag aacagcaaat cgccgggttt
    58801 gagatcaggt gccgttattt cttttattga ttgttcggtg aaagagcttt gatgctggaa
    58861 tttgacagcc catgtttttg cctcggcatc tactgctgtg gctgatgggt ctggctggct
    58921 gatatcaacg gtacaagccg aaagtaaaag aaagctgggg agaagcaggc ggcagtacgc
    58981 ctttggttta tccattttat acaatccatg taaaaaaggg ccctgaaatt caggaccctt
    59041 tctggcatca gcctttaatc tgtttcacca gatattcgac gatgtcacca gtcttaatta
    59101 actgtttctc gccgttgcga cgatatttat attcgatatc gtcgttgtcg aggttacggt
    59161 cgcccagcac aatagtgtgc ggaataccga tcagttccat atcagcaaac atcacgcccg
    59221 gacgctcttt gcggtcatcc agcagcactt cgataccttg tgcacgcagt tcgctgtaca
    59281 gtttctcagc aagctcttgt acgcggaagg atttgtgcat gttcatcggc agaatcgcca
    59341 cctggaacgg cgcgatagcg tcaggccata cgatgccgcg ttcgtcgtag ttctgctcaa
    59401 tcgccgcagc taccacacgc gttaccccga taccgtagca acccatcgtc aggatttggt
    59461 tacggccatc ttcaccctgt acggaggctt tcagtgcttc ggagtacttg gtacccagct
    59521 ggaagatgtg accaacttcg ataccacgtt tgatcagcag cgtaccctgg ccatccgggc
    59581 ttggatcgcc agccaccacg ttacggatat ctgcaacttc cggggtagcg acatcgcgat
    59641 cccagttgat accgaagtag tgtttaccat cgatgttagc accagcagcg aaatcactca
    59701 tcgccgcaac ggtacggtca atcaccaccg gaatcggcat gtttaccgga cccagtgaac
    59761 ccggaccggc tttaaccaca gcacgaattt cttcttcggt cgcgaaagtc agcgggcttg
    59821 caacctgcgg cagtttttct gctttaactt cgttcagctc gtgatcaccg cgcaccagca
    59881 gcgcaaccag cgggaagcta ctgccttcaa ccgctttaac cagcagagtc ttaaccgttt
    59941 tctcaatcgg cagattgaac tgttcaacca gttccgcgat ggttttcgcg ttcggcgtat
    60001 caaccagcgt catttcctgg gtagcagcag cgcgcggttc tttcggcgcg atagcttctg
    60061 ccagttcaat gttcgctgca tagtcagagg tgtcggagaa gaccacatcg tcttcaccgc
    60121 tctgcgccag cacctggaat tcgtgagagg cgctgccgcc gatagaaccg gtgtcggcct
    60181 gtacggcgcg gaaatccagc cccatgcggc tgaagatttt gctgtaggcc gcatacattg
    60241 catcgtaggt ttcctgcagg gattcctgag aagtatggaa agagtaagca tctttcatca
    60301 ggaattcgcg ggaacgcatg acgccgaaac gcggacgcac ttcgtcgcgg aacttggtct
    60361 ggatctgata gaagttcagc ggcagctgtt tgtaagagct aagctcgtta cgaatcaggt
    60421 cagtgataac ttcttcatga gttgggccga gtacgaacgg acgctcgcca cggtcaacaa
    60481 aacgcagcag ttccggaccg tactgttccc aacgaccact ctcttgccac aaatcggctg
    60541 gctgaaccac cggcatcgac acctcgatcg caccggcgtt gttcatctct tcacgcacga
    60601 tgttttcgac ttttttcaga acgcgcacgc cggtcggcag ccaggtatat aacccggagg
    60661 ccagcttgcg gatcatcccg gcgcgcagca tcagctgatg gctgatcacc tcggcgtcgg
    60721 caggtgtctc cttgagagtg gagagcaggt attggctagt acgcatgttg ttacggttcc
    60781 agttggaagg tagaacaggc tcaaggcgag cctgggacaa aaaaagtgat ttagtttacc
    60841 agtgcaaaag aaatgtcaaa agagaagggc gtgaatttaa cgcggttcca gcgcaaagac
    60901 ttcaaaacct gcgtcggtga cgcgccagcg aacgttaaaa tcatgtagcc agacggcata
    60961 ggttttgccc gtttcctcac ctttacgata ggccgggcgc gggtcctgcg ccagtacttc
    61021 gcggataaac agcgttaact gcggataacg cttctccagc gtcaaaagct gcttttcgac
    61081 ctctgcggta aaactcaccg ccatctctgc agctggcgca ctttgcgcat agctggcact
    61141 ggcatcggga agcgattcgg caaaggggag atacggtttg atatccacta ccggcgtacc
    61201 atcgaccaga tccagactgc cgagcttcag aatcacgctg cctttatggc aaacaacctc
    61261 tttcagctct accagcgaca tgccaattgg gttagggcgg aaagtagagc gtgttgcgaa
    61321 aacccccatt ctggcgttac cgccgaggcg cggtggacgc acagtcggac gccagccgcc
    61381 ttccatcgtt tgatgaaaga cgaaaaggat ccataaatgg ctgaatgctt ccaggccgcg
    61441 aacggcgtcg gcctggttgt aggaagctat gagatgcagt tcgccgttgg cgctttttac
    61501 cagacctggc tggcgcggaa cggcgaattt ttctttatag ggcgagcgaa taacgcctat
    61561 ttgctcgaac tggaaactgc tcatttcgcc gtaatgttaa gcgcagagcc gatacataca
    61621 gcctgacgat agcagcccgg cgtaccgctg gtgacttcgc agctatgcag taatacagca
    61681 ttggctttca ttttagaggc attgatttgc atctgcttac gtgcggttgg aatgctcggc
    61741 ggagagtcct gattagaggc ctggcaagag tcgccactga cttcaccgag atcgcggaac
    61801 ggtttgccga ctaattcttc tgcattggta taaattcgga ccggcgtggc gcgcggcgct
    61861 ttcggttttg caggctccgc tttcggctgg ggtgcagtgc tttgaacggg ttcgacaggg
    61921 gatctgctta acatggaaca gccgcttagc atgagtgcta ctaaacagat cggtaaagca
    61981 cgcatagtat ttcctcaatg tatgatcaaa acgtcaatat tgaatcagga gcttgtaaaa
    62041 atgacaagac gggcaagcgc ccgtcctgaa tgatattaca aattgtggaa acagcctaaa
    62101 aattaccagc ctttaacagc gccgccgtta aacactttgt ttgctgcttc gtaaacttca
    62161 tcagactgat aagcctggac gaatttcttc acgttctcgg cgtctttgtt atcttcacgc
    62221 gttacgatca ggtttacgta tggggagtct ttatcttcga caaagatacc gtctttcgcc
    62281 ggagtcaggc caatctggct ggcataggtg gtattgataa ctgccagagc gatttgcgcg
    62341 tcgtccagag aacgcggcag ttgcggtgct tccagttcaa caattttcag attttttggg
    62401 ttctcaacaa catcaagaac ggtcggcagc aggccaacgc catctttcag tttgatcaag
    62461 cccacttttt gcagcagcag cagtgaacga ccaagattag ttggatcgtt tggcacggca
    62521 acctgcgaac catcctgcag ttcatccagt gatttgattt tcttggagta accagcaatc
    62581 ggataaacaa aagtgttgcc tactgcgacc agtttgtagc cacgatcttt cagttgctga
    62641 tcaaggtacg gtttatgctg gaaggcgttg gcgtcgatat cgcctttgct caatgcttcg
    62701 tttggcagaa catagtcgtt gaaggttacc agctcaacgt ccaggccata tttgtctttc
    62761 gcaactttct gcgcgacttc tgctacctgc tgttcggcac caacaatcac gccgacttta
    62821 atgtggtttg gatctttttc atcctgaccg cagcctacca gtgccagtga tccaatcagg
    62881 gctcccactg ccgcaaaggt tttgaatttg aacgccatac cttattcctt cttcaattat
    62941 ttatgttgtg ttgaacgtta cttgcgagtg acagcccgga cgatgcggtc gcctgcaaac
    63001 tgaattaaat aaaccagaat gaccagcaat accagtaccg tattcatcac cgtcgcgttg
    63061 tagccgatgt agccatactg atagccaatc tgacctaaac caccggcacc gactgcacca
    63121 cccatcgcgg aataaccgac cagggtaatc agggtgatag ttgccgcatt caccagaccc
    63181 ggcaacgctt ccggtaacag cactttacag acgatctgca ttggcgtggc acccattgcg
    63241 cgggaagctt caattaaccc ggttgggatc tccagcagag cgttctcgac catacgggca
    63301 ataaacggtg ctgcaccaac ggttaacgga acaattgctg cctgcaaacc aatcgatgta
    63361 ccgacaataa cgcgggtaaa cggaatcatc cataccagca agataatgaa cgggatggaa
    63421 cggaaaatgt tcacaatcgc agaaacggta cgatacagct tcgcgttagc aataatttgc
    63481 cccggacgcg tgacataaag cagaacgcca accggcagac caatcacaaa cccaaaaaat
    63541 ccggatacga aggtcattgc cagcgtttcc catacgccac gaaccagcag ccacatcatc
    63601 ggctcagaca taacccagta cctctacttt tacatggtgt tcctgcagcc aggcaatggc
    63661 ggcttgcgta tcttgttgtg tgccgtgcat ttcagtcagc atgatgccga atttaacgcc
    63721 accggcgtag tccatctgcg cgctaataat gttgttgttg acgttgaaac gacgcgcggt
    63781 ttcagaaagc agtggggcat cgaccgattg accggtaaac tccagacgca gcatcggcac
    63841 gcagtcagta aatggctccg tttgcagacg ttcctggtaa tcttccggga tatccagatg
    63901 cagggtcgac tgaataaact tctgcgccag cggcgttttc ggatgcgaga acacttcact
    63961 taccgtgtcc tgctcgatca gttcgccatt gctgatgacc gccacgcaat cacaaatacg
    64021 cttcacaacg tccatttcgt gggtgatcaa cagaatcgtc aaacccagac ggcggttaat
    64081 gtctttcagc agttcgagaa tagaacgtgt cgttgccggg tccagcgcgc tggtggcttc
    64141 atcacacagc aatactttgg gattgctggc taatgcacgg gcaatcgcca cacgttgttt
    64201 ctgcccaccg gaaagattcg acgggtagct atcatgctta tcgccaagac caaccaatga
    64261 cagcaattcc gtcacgcgac gtttgatctc gtctttcggt gtgttgtcca gctccagcgg
    64321 cagagccacg ttgccaaaaa cagtacgcga agagagcagg ttaaaatgct ggaaaatcat
    64381 accaatctgg cggcgagctt tggtcaactc ggattctgac agcgtggtca attcctggcc
    64441 atcgaccagc acgctaccct cggttgggcg ctccagcaag tttacacaac gtataagcgt
    64501 actcttaccc gcgcctgagg caccgataac gccataaatt tgtccagctg gcacatgcag
    64561 gctgacgttg ttcaacgcct ggatggtgcg ggtgccctgg tggaacactt tggtgatatt
    64621 cgaaagtttt atcattgatt atttattatc gtcattaagt tagtcgtagc atctcgaatg
    64681 cctgaaacgg gcaacgccgt caatgaaatg gatgttaagg catccagacg tctaaatcaa
    64741 tcaggtttat gcgaagagca ctttcttgca ggtcgaaaca tgcgatacta gcgtcccatg
    64801 ccttattaag gagctataaa aggtggcgaa gagcgtaccc gcaatttttc ttgaccgtga
    64861 tggcaccatt aatgtcgatc acggctatgt ccatgagatc gacaactttg aatttatcga
    64921 cggtgttatt gacgccatgc gcgagctaaa aaaaatgggc tttgcgctgg tggtagtaac
    64981 caaccagtct ggcattgctc gcggtaaatt taccgaagca cagtttgaaa cgctgaccga
    65041 gtggatggac tggtcgctgg cggaccgaga tgtcgatctg gatggtatct attattgccc
    65101 gcatcatccg caggggagtg ttgaagagtt tcgccaggtc tgcgattgcc gcaaaccaca
    65161 tccggggatg cttttgtcag cacgcgatta tttgcatatt gatatggccg cttcttatat
    65221 ggtgggcgat aaattagaag atatgcaggc agcggttgcg gcgaacgtgg gaacaaaagt
    65281 gctggtgcgt acgggtaaac ctattacacc tgaagcagaa aacgcggcag attgggtgtt
    65341 aaatagcctg gcagacctgc cgcaagcgat aaaaaagcag caaaaaccgg cgcaatgatt
    65401 aaaagatgaa cggttgaaat aaaaatgcat ttttccgctt gtcttcctga gccgactccc
    65461 tataatgcgc ctccatcgac acggcggatg tgaatcactt cacacaaaca gccggttcgg
    65521 ttgaagagaa aaaatcctga aattcagggt tgactctgaa ataggaaagt gtgatatacg
    65581 ccacctcgcg acagtgagct gaaagc
//
LOCUS       DAJTSA010000011        64194 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000011 DAJTSA010000000
VERSION     DAJTSA010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64194
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.011"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            131..574
                     /locus_tag="OO369_000742"
     CDS             131..574
                     /locus_tag="OO369_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5981125.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            810..1628
                     /gene="ybhA"
                     /locus_tag="OO369_000743"
     CDS             810..1628
                     /gene="ybhA"
                     /locus_tag="OO369_000743"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR5981126.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1629..2687)
                     /gene="modC"
                     /locus_tag="OO369_000744"
     CDS             complement(1629..2687)
                     /gene="modC"
                     /locus_tag="OO369_000744"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR5981127.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2690..3379)
                     /gene="modB"
                     /locus_tag="OO369_000745"
     CDS             complement(2690..3379)
                     /gene="modB"
                     /locus_tag="OO369_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR5981128.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3379..4152)
                     /gene="modA"
                     /locus_tag="OO369_000746"
     CDS             complement(3379..4152)
                     /gene="modA"
                     /locus_tag="OO369_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5981129.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVANKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="OO369_000747"
     CDS             complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="OO369_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR5981130.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4597..5385
                     /gene="modE"
                     /locus_tag="OO369_000748"
     CDS             4597..5385
                     /gene="modE"
                     /locus_tag="OO369_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR5981131.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5453..6925
                     /gene="modF"
                     /locus_tag="OO369_000749"
     CDS             5453..6925
                     /gene="modF"
                     /locus_tag="OO369_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR5981132.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7186..8202
                     /gene="galE"
                     /locus_tag="OO369_000750"
     CDS             7186..8202
                     /gene="galE"
                     /locus_tag="OO369_000750"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR5981133.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8212..9258
                     /gene="galT"
                     /locus_tag="OO369_000751"
     CDS             8212..9258
                     /gene="galT"
                     /locus_tag="OO369_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR5981134.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9262..10410
                     /gene="galK"
                     /locus_tag="OO369_000752"
     CDS             9262..10410
                     /gene="galK"
                     /locus_tag="OO369_000752"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR5981135.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10404..11444
                     /gene="galM"
                     /locus_tag="OO369_000753"
     CDS             10404..11444
                     /gene="galM"
                     /locus_tag="OO369_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR5981136.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11647..12399
                     /gene="gpmA"
                     /locus_tag="OO369_000754"
     CDS             11647..12399
                     /gene="gpmA"
                     /locus_tag="OO369_000754"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR5981137.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="OO369_000755"
     CDS             complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="OO369_000755"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR5981138.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13934..14314
                     /locus_tag="OO369_000756"
     CDS             13934..14314
                     /locus_tag="OO369_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR5981139.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14428..15369
                     /gene="zitB"
                     /locus_tag="OO369_000757"
     CDS             14428..15369
                     /gene="zitB"
                     /locus_tag="OO369_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR5981140.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="OO369_000758"
     CDS             complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="OO369_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR5981141.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="OO369_000759"
     CDS             complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="OO369_000759"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR5981142.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17415..17490)
                     /locus_tag="OO369_000760"
     tRNA            complement(17415..17490)
                     /locus_tag="OO369_000760"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17455..17457),aa:Lys,seq:ttt)
     gene            complement(17494..17569)
                     /locus_tag="OO369_000761"
     tRNA            complement(17494..17569)
                     /locus_tag="OO369_000761"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17534..17536),aa:Val,seq:tac)
     gene            complement(17621..17696)
                     /locus_tag="OO369_000762"
     tRNA            complement(17621..17696)
                     /locus_tag="OO369_000762"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17661..17663),aa:Lys,seq:ttt)
     gene            complement(17699..17774)
                     /locus_tag="OO369_000763"
     tRNA            complement(17699..17774)
                     /locus_tag="OO369_000763"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17739..17741),aa:Val,seq:tac)
     gene            complement(17910..17985)
                     /locus_tag="OO369_000764"
     tRNA            complement(17910..17985)
                     /locus_tag="OO369_000764"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17950..17952),aa:Lys,seq:ttt)
     gene            complement(18150..18941)
                     /gene="cpoB"
                     /locus_tag="OO369_000765"
     CDS             complement(18150..18941)
                     /gene="cpoB"
                     /locus_tag="OO369_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR5981143.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18951..19472)
                     /gene="pal"
                     /locus_tag="OO369_000766"
     CDS             complement(18951..19472)
                     /gene="pal"
                     /locus_tag="OO369_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR5981144.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19507..20799)
                     /gene="tolB"
                     /locus_tag="OO369_000767"
     CDS             complement(19507..20799)
                     /gene="tolB"
                     /locus_tag="OO369_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR5981145.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20932..22173)
                     /gene="tolA"
                     /locus_tag="OO369_000768"
     CDS             complement(20932..22173)
                     /gene="tolA"
                     /locus_tag="OO369_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR5981146.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22238..22666)
                     /gene="tolR"
                     /locus_tag="OO369_000769"
     CDS             complement(22238..22666)
                     /gene="tolR"
                     /locus_tag="OO369_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR5981147.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22670..23362)
                     /gene="tolQ"
                     /locus_tag="OO369_000770"
     CDS             complement(22670..23362)
                     /gene="tolQ"
                     /locus_tag="OO369_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR5981148.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23359..23763)
                     /gene="ybgC"
                     /locus_tag="OO369_000771"
     CDS             complement(23359..23763)
                     /gene="ybgC"
                     /locus_tag="OO369_000771"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5981149.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23913..24206)
                     /gene="ybgE"
                     /locus_tag="OO369_000772"
     CDS             complement(23913..24206)
                     /gene="ybgE"
                     /locus_tag="OO369_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR5981150.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24206..24319)
                     /gene="cydX"
                     /locus_tag="OO369_000773"
     CDS             complement(24206..24319)
                     /gene="cydX"
                     /locus_tag="OO369_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR5981151.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24334..25473)
                     /gene="cydB"
                     /locus_tag="OO369_000774"
     CDS             complement(24334..25473)
                     /gene="cydB"
                     /locus_tag="OO369_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5981152.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25489..27057)
                     /gene="cydA"
                     /locus_tag="OO369_000775"
     CDS             complement(25489..27057)
                     /gene="cydA"
                     /locus_tag="OO369_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR5981153.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27906..30539)
                     /gene="mngB"
                     /locus_tag="OO369_000776"
     CDS             complement(27906..30539)
                     /gene="mngB"
                     /locus_tag="OO369_000776"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCR5981154.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(30557..32473)
                     /gene="mngA"
                     /locus_tag="OO369_000777"
     CDS             complement(30557..32473)
                     /gene="mngA"
                     /locus_tag="OO369_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR5981155.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            32643..33365
                     /gene="mngR"
                     /locus_tag="OO369_000778"
     CDS             32643..33365
                     /gene="mngR"
                     /locus_tag="OO369_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR5981156.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(33468..34337)
                     /gene="sucD"
                     /locus_tag="OO369_000779"
     CDS             complement(33468..34337)
                     /gene="sucD"
                     /locus_tag="OO369_000779"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR5981157.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(34337..35503)
                     /gene="sucC"
                     /locus_tag="OO369_000780"
     CDS             complement(34337..35503)
                     /gene="sucC"
                     /locus_tag="OO369_000780"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR5981158.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(35597..36814)
                     /gene="odhB"
                     /locus_tag="OO369_000781"
     CDS             complement(35597..36814)
                     /gene="odhB"
                     /locus_tag="OO369_000781"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR5981159.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36829..39630)
                     /gene="sucA"
                     /locus_tag="OO369_000782"
     CDS             complement(36829..39630)
                     /gene="sucA"
                     /locus_tag="OO369_000782"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR5981160.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39931..40647)
                     /gene="sdhB"
                     /locus_tag="OO369_000783"
     CDS             complement(39931..40647)
                     /gene="sdhB"
                     /locus_tag="OO369_000783"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR5981161.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40663..42429)
                     /gene="sdhA"
                     /locus_tag="OO369_000784"
     CDS             complement(40663..42429)
                     /gene="sdhA"
                     /locus_tag="OO369_000784"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR5981162.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(42429..42776)
                     /gene="sdhD"
                     /locus_tag="OO369_000785"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(42429..42776)
                     /gene="sdhD"
                     /locus_tag="OO369_000785"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR5981163.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42770..43174)
                     /gene="sdhC"
                     /locus_tag="OO369_000786"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42770..43174)
                     /gene="sdhC"
                     /locus_tag="OO369_000786"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5981164.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            43555..43662
                     /locus_tag="OO369_000787"
     CDS             43555..43662
                     /locus_tag="OO369_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981165.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43868..45151
                     /gene="gltA"
                     /locus_tag="OO369_000788"
     CDS             43868..45151
                     /gene="gltA"
                     /locus_tag="OO369_000788"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR5981166.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            45542..46108
                     /locus_tag="OO369_000789"
     CDS             45542..46108
                     /locus_tag="OO369_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5981167.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            46159..48618
                     /locus_tag="OO369_000790"
     CDS             46159..48618
                     /locus_tag="OO369_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5981168.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            48633..49364
                     /locus_tag="OO369_000791"
     CDS             48633..49364
                     /locus_tag="OO369_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5981169.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            49361..50437
                     /locus_tag="OO369_000792"
                     /pseudo
     CDS             49361..50437
                     /locus_tag="OO369_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            50589..51635
                     /locus_tag="OO369_000793"
     CDS             50589..51635
                     /locus_tag="OO369_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR5981170.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(51632..52423)
                     /gene="nei"
                     /locus_tag="OO369_000794"
     CDS             complement(51632..52423)
                     /gene="nei"
                     /locus_tag="OO369_000794"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR5981171.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(52459..53193)
                     /gene="pxpA"
                     /locus_tag="OO369_000795"
     CDS             complement(52459..53193)
                     /gene="pxpA"
                     /locus_tag="OO369_000795"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR5981172.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(53183..54115)
                     /gene="pxpC"
                     /locus_tag="OO369_000796"
                     /pseudo
     CDS             complement(53183..54115)
                     /gene="pxpC"
                     /locus_tag="OO369_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(54109..54765)
                     /gene="pxpB"
                     /locus_tag="OO369_000797"
     CDS             complement(54109..54765)
                     /gene="pxpB"
                     /locus_tag="OO369_000797"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR5981173.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(54788..55531)
                     /gene="ybgI"
                     /locus_tag="OO369_000798"
     CDS             complement(54788..55531)
                     /gene="ybgI"
                     /locus_tag="OO369_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR5981174.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            55802..57283
                     /gene="dtpD"
                     /locus_tag="OO369_000799"
     CDS             55802..57283
                     /gene="dtpD"
                     /locus_tag="OO369_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR5981175.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(57433..58851)
                     /gene="phrB"
                     /locus_tag="OO369_000800"
     CDS             complement(57433..58851)
                     /gene="phrB"
                     /locus_tag="OO369_000800"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR5981176.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(58848..59357)
                     /locus_tag="OO369_000801"
     CDS             complement(58848..59357)
                     /locus_tag="OO369_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR5981177.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            59486..59773
                     /locus_tag="OO369_000802"
     CDS             59486..59773
                     /locus_tag="OO369_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCR5981178.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(59828..60208)
                     /locus_tag="OO369_000803"
     CDS             complement(59828..60208)
                     /locus_tag="OO369_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981179.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(60211..63597)
                     /locus_tag="OO369_000804"
     CDS             complement(60211..63597)
                     /locus_tag="OO369_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5981180.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            complement(63616..63819)
                     /locus_tag="OO369_000805"
                     /pseudo
     CDS             complement(63616..63819)
                     /locus_tag="OO369_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        15693 a        17276 c        15621 g        15604 t
ORIGIN      
        1 tgccgaagaa gatccagctt ctgcaaccag gccattcaga actgaagtga agcgcagtcg
       61 actgttaatt gacgagtatc tgttaatcag gaaaatagca gacgtccaga acgaatggtt
      121 cggcctttgc atggatcttg cactggtaac aggacagcgc aaaggagatc tggcggctat
      181 gagatgggag gatataagag atagcaggct atacgtggaa caacaaaaaa caggggcaaa
      241 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      301 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      361 gacaattgca gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga
      421 cccaccgcca tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg
      481 cagtgatttc actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg
      541 tgacggacga gggagggaat ggaaagacat ttagaattaa tttcgaatga tttcgaaatg
      601 ccacttgtaa ttaattgatt ataaagaatt aattcctaaa gagcatttgc ttcatgaatg
      661 ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg
      721 ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga
      781 aaattcttaa ccttaatctg gaatacgcca tgaccacacg cgtgattgct ctcgacttag
      841 acggcacctt attgaccccg aaaaagacct tgcttccatc atcgatagaa gccctggccc
      901 gcgctcgcga agcaggctat caattaatca tcgtcacagg tcgccatcac gtcgctattc
      961 atccttttta tcaggcgctg gcgctggata cacctgctat ttgctgtaat ggcacctatt
     1021 tgtatgatta tcatgcaaaa accgtgctgg aagcggaccc aatgcccgtt aataaagccc
     1081 tgcaactcat tgagatgctg aatgaacacc acattcacgg tctgatgtat gtcgatgatg
     1141 caatggtcta tgagcacccg accgggcatg tcattcgcac gtctaactgg gcgcaaaccc
     1201 tgccgccgga acagcgtccg actttcacac aagtcgcttc tctggctgaa acgacgcaac
     1261 aagttaacgc cgtatggaag ttcgccctga cgcacgatga cctgccgcaa ttgcagcatt
     1321 ttggtaagca tgtcgaacat gaactgggac tggagtgtga atggtcctgg cacgatcagg
     1381 ttgatattgc acacggcggc aacagcaaag gtaaacgttt gacgaaatgg gttgaggcgc
     1441 aaggttggtc gatggaaaac gtcgtggcat tcggcgataa ctttaatgat atcagtatgc
     1501 tggaagccgc tggtacaggc gtggcgatgg gcaacgccga tgacgcggta aaagcgcgcg
     1561 ccaacattgt gattggtgat aacaccaccg acagcattgc ccagttcatt tatagccacc
     1621 tgatttaatc aggcggttat cgacacactt ttaatttgcg cgtacagcca caggccaggt
     1681 ttgatcgcca gttcatccct ggcccacggg ctgatacgcg cccacagcgt tttaccgccg
     1741 acttccagct ccacttccac ctggccgttg tcgtcataac tattaacaac ttttgcccgc
     1801 aatacgttac gaatgctggt ttgctgcggc ggttgtaaaa ccagagaaac atcggaagcc
     1861 tgaatgcgga tacgtagcgc agcttgcagc ggttcgccca gcttattgac ccacaaatgc
     1921 tgatcgccca gcgccagcgc ggtcatcgcg taatgcggat gatgctcaag caccgtcact
     1981 ttcagaatgc tactttgttg ctctttcggc agccacggat tcatcacgct actgccccac
     2041 acttcctcca gcgcgccaaa ggctttcacc tgaccgtttt ccagtaccat cactctgtct
     2101 gccagatgga ggatctcatc cagcgaatgg ctgacataca acatcggaat gttgatttcc
     2161 cgtgtcagcc gttgcagata aggcaacagt tcgcgtttac gcggaatatc cagtgacgcc
     2221 agcggttcat ccagcaacag caattccggt gctgtcagca aagcccgacc aatcgccacg
     2281 cgctgttttt cgcctccgga caggctgcct ggtaaacggt caagcaacgg ttcaatgcct
     2341 aaaagcgcca ccagcttatc gaactgatcg accatacttt tcgacatgcc gtagcgcaga
     2401 ttgccacgca ctttgtaatg cgggaacaac cgcgcatcct gaaaaacata gccaacacga
     2461 cgcttttccg gcgtcaggca gatacctttt tcggcatcat ttagtacccg cccattgagg
     2521 acaatccgcc ctttttgcgg gcgcgtcagt ccactgatgg cgttaatcag cgaagttttt
     2581 ccggcaccgg agacgccaaa gatagcagtg atgccattgg cgggcagcgt ttcattaatg
     2641 gtcaggcaat ggttgcccaa cgtctgggaa aaattcagct ccagcatgat tagcgccccg
     2701 cccgttcacg gctgattctg gccagccatt ctgaaatcaa cagggagatc atcgccagcg
     2761 caatagaaat aatgcacagt ctcgccgcgc cactttcccc gccgggggtc tggatcaggg
     2821 tatacatggc agaaggaatg gttcgcgttt cgctaggaat gttcgacaca aaggtgatgg
     2881 ttgcaccaaa ctcaccgaga gaacgagcaa aagccagtac cgtaccaaca ataattcccg
     2941 gtaaggtcag cggtaacgtg atagtaaaga aaacgcgcca gcgcccggca cctagtgttc
     3001 ttgcggcctg ttccagtttg acatcaaccc cttccagcgc cagacgaatt gcccgcacca
     3061 tcagcggaaa cgacatgacg gcggcagcga gaaccgcgcc gcgccagcta aaggcgaagg
     3121 taataccaaa ccagtcatac agacgttcac cgataaatcc gcgccgtccc atcgaaacta
     3181 ataataagta accgacgacc acgggcggta acaccagcgg tagatgcagt acgctgtcga
     3241 gcagagcttt gcccggaaac gtgcaacgca ccagtaacca ggcaaaaaag atcccaaacg
     3301 gcaggctaaa cagcacagcc agggaagaaa ctttcaggct taataaaact gcctgccatt
     3361 ctggatcggt cagtatcatt acttggttgt aaatccgtaa cgtttaaaga tttccgctgc
     3421 ctgcggtccc ttcagataat cataaaatgc tttcactgtc gcattgttat gcccttccac
     3481 aacagcaacc ggatattcca cttttttatg tgaatcttcc gggaaggtgg caaccacttt
     3541 tacccctttg ttggcaactg cgtcagaacc gtagacaatg cccagaggcg cttcgttacg
     3601 ttcgaccagc gccagcgccc cacgaacatc ttccgctggg gccagtttcg gagagagcgt
     3661 atcccatgcg cccagttttt gcagtgcttc ttttgcataa atgccagcgg gaacatgttc
     3721 cggatcgcca accgccaggc gaccgccatt cagcagcgaa gtccagttag ttttgctgtc
     3781 gatggtgaaa tctttctgct cgctggcttt cggtgctaca acgaccaggc tattgccgag
     3841 cagtgtctga cgcgtagcag tatcgatcgc ttttttatca accgcataat ccatccattt
     3901 ctgatcggca gaaataaaca gatccgcagg ggcacccgct tcaatctgac gggcgagagt
     3961 agatgacgaa gcgaaagaag aaaccacatc cacgcctttc tcttttttat actgcgtagc
     4021 aatgtcctgc attgcgttag tcagtgatgc ggcggcgaac accgtgattt tcccttcatc
     4081 tgccagtgca ttgccagcaa ctgcgaaaga gagtgccgcc ccggcaaaca agttcaacca
     4141 tttacgagcc attggtaacc ccttaatgta acgttatgta ggcgacaata taacgactaa
     4201 ctcggaattt tcccagcagt tattgctaac ctttgtgagg tagataagaa aaaatatcgg
     4261 caggaaaagc aggaagttga gaaaaagaaa atgcccgact aagcgggcac tcagggaatc
     4321 aatgattttg tccgggctgg tcttttttac caacaccaga aaagatgttg aatacttcac
     4381 caagaccgta aatcagaccc aggatgatgg ccatcacgac aggtaccatg attacggcga
     4441 ataccagact ttttaataac tctaacatgg tcaactccag atatagtcat gagactattc
     4501 taaccgctaa gcacagaaaa gcactcccct tttgtgcggt cagctttgcg tggcgttcgt
     4561 tttccgtcac aataagactt ttgccaggac attgttatgc aggccgaaat ccttctcacc
     4621 cttaagctcc aacaaaaatt attcgccgac ccgcgccgca tttcgctact aaaacacatt
     4681 gcgctttccg gttccattag ccagggagcg aaagatgccg gtatcagcta taaaagcgcc
     4741 tgggatgcca ttaacgagat gaatcagtta agtgagcata ttctggtcga gcgcgcaaca
     4801 ggcggtaaag gtggcggagg cgcagtactg acccgctatg gtcagcgact gattcagctc
     4861 tatgacttac tggcgcaaat ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc
     4921 ctgccgctga acagcctgct ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt
     4981 aaccagtggt tcggtaccat caccgcccgc gatcatgatg acgttcaaca gcatgttgat
     5041 gtcttactgg ctgacggaaa aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca
     5101 cgtctggggc tggatgaagg caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc
     5161 attactcagg acgaagcggt cgcacaaaac gccgacaacc aattaccggg tattattagt
     5221 catattgagc gcggcgcaga gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca
     5281 ctgtgcgcca cagtgccggt aaatgaagcg acttctcttc agcaagggca gaatgtcacg
     5341 gcctacttta atgccgacag cgtgattatc gccacgctgt gctaagcgtg ttgacaattt
     5401 gttatgaaac acgtatccct gtcagtaatc gctgcacaaa atgggatata aaatgtcatc
     5461 gttgcaaatt ttgcaaggca cgtttcgtct tagcgacaca aaaacgctgc aattgcctca
     5521 gctaacgtta aacgcgggtg atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc
     5581 ggcgctggcc cgcgcgctgg cgggggaact tccgcttttg aaaggtgaac ggcaaagcca
     5641 gttttcccac atcactcgtc tctccttcga gcaattgcaa aagctcgtca gcgacgaatg
     5701 gcaacggaat aacaccgata tgctcggccc tagcgaagat tacaccggac gcactacggc
     5761 tgagattatt caggatgaag taaaggatgc accgcgttgc atgcaactgg cgcagcagtt
     5821 cggtattacc gccctcctcg accgacgctt taaatacctt tccactggcg agacgcgaaa
     5881 aaccctgctg tgtcaggcgc tgatgtcgga gcctgacttg ttgattcttg atgagccgtt
     5941 cgatggcctg gatgttgcct cacgtcagca gctggctgag cgactcgcct cgttacatca
     6001 gtccggtatt actttggtac tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca
     6061 gtttgctggc gtgctggcgg attgtacgtt agcggaaact ggcgctaaag aggaactgct
     6121 ccagcaagca ctcgtcgcgc aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc
     6181 ggagccggat gaaccttcag cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct
     6241 gaacaatggc gtggtttctt ataatgatcg ccccattctt aataacctta gctggcaggt
     6301 gaatccaggc gaacactggc aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt
     6361 aagcctgatt actggcgatc atccgcaagg ttacagcaac gatttgacgc ttttcggccg
     6421 acgtcgcggc agcagcgaaa ccatctggga catcaaaaag catatcggtt acgtcagcag
     6481 tagtttgcat ctggattacc gggtcagcac taccgtgcgt aatgtgattc tttctggcta
     6541 ttttgattcg attggcattt atcaggtcgt ttcggatcgc cagcaaaaac tggtgcagca
     6601 gtggctggat attctcggca ttgataaaca cacggctgac gctccgttcc atagtctttc
     6661 ctggggacag cagcgtttgg cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct
     6721 tattctcgat gaaccactac aggggcttga tccgctcaat cgccaactta tccgccgttt
     6781 tgttgatgtg ctgattagcg aaggtgaaac gcaattgttg tttgtttcgc accacgctga
     6841 agatgcgcct gcctgtatta cccatcgcct tgagttcgtg ccggacggtg gactctatcg
     6901 ctatgtgctg acaaaaatat attgagtcgg tagtgctgac cttgccggag gcggcctaag
     6961 cgcactctcc ggccaacggt tcgacgcatg caggcatgaa accgcttctt ttttcagata
     7021 aaaagcgcaa tcattcataa accctctgtt ttataatcac ttaatcgcgc ataaaaaacg
     7081 gctaaattct tgtgtaaacg attccactaa tttattccat gtcacacttt tcgcatcttt
     7141 gttatgctat ggttatttca taccataagc ctaatggagc gaattatgag agttctggtt
     7201 accggtggta gcggttacat tggaagtcat acctgcgtgc aattactgca aaacggtcat
     7261 gatgtcatca ttcttgataa cctctgtaac agtaagcgca gcgtactgcc tgttatcgag
     7321 cgtttaggcg gcaaacatcc aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg
     7381 accgagatcc tgcacgatca cgctatcgac accgtgatcc acttcgccgg gctgaaagcc
     7441 gtcgccgaat cggtacaaaa accgctggaa tattacgaca acaatgtcaa cggcactctg
     7501 cgcctgatta gcgccatgcg cgccgctaac gtcaaaaact ttatttttag ctcctccgcc
     7561 accgtttatg gcgatcagcc ccaaattcca tacgttgaaa gcttcccgac cggcacaccg
     7621 caaagccctt atggcaaaag caaattgatg gtggaacaga tcctcaccga cctgcaaaaa
     7681 gcccagccgg actggagcat tgccctgctg cgctacttca acccggttgg cgcgcatccg
     7741 tcgggcgata tgggcgaaga tccgcaaggc attccgaata acctgatgcc atacatcgcc
     7801 caagttgctg taggccgtcg cgactcgctg gcgatttttg gtaacgatta tccgaccgaa
     7861 gatggtactg gcgtacgcga ttacatccac gtaatggatc tggcggacgg tcacgtcgtg
     7921 gcgatggaaa aactggcgaa caagccaggc gtacacatct acaacctcgg tgctggcgta
     7981 ggcagcagcg tgctggacgt ggttaatgcc ttcagcaaag cctgcggcaa accggttaac
     8041 tatcattttg caccgcgtcg cgaaggcgac cttccggcct actgggcgga cgccagcaaa
     8101 gccgaccgtg aactgaactg gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc
     8161 tggcactggc agtcacgcca tccacaggga tatcccgatt aaggaacgac catgacgcaa
     8221 tttaatcccg ttgatcatcc acatcgccgc tacaacccgc tcaccgggca atggattctg
     8281 gtttcaccgc accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc agccaaacag
     8341 gtgttacctg cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc
     8401 gataaaaacc ccgattacac cgggacttac gttttcacta atgactttgc ggccttgatg
     8461 tctgacacgc cagatgcgcc agaaagcaac gatcctttga tgcgttgcca gagcgcgcgt
     8521 ggtaccagcc gggtgatctg cttttcaccg gatcacagta aaacgttgcc agaactgagc
     8581 gttgcggcat tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa
     8641 acgtacccgt gggtgcaggt ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg
     8701 catccgcacg gacagatttg ggcaaatagc ttcctgccta acgaagctga gtgcgaagac
     8761 cgcctgcaaa aagaatattt tgccgaacag aaatcaccaa tgctggtgga ttatgttcag
     8821 cgcgagctgg cagacggtag ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg
     8881 ccttactggg ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg
     8941 atcaccgatt tgaccgacgc ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc
     9001 agtcgttatg acaacctctt ccagtgctcc ttcccctact ctatgggctg gcacggcgca
     9061 ccatttaatg gcgaagagaa tcaacactgg cagctgcacg cgcactttta tccgcctctg
     9121 ctgcgctccg ccaccgtacg taaatttatg gttggttatg aaatgctggc agagacccag
     9181 cgagacctga ccgcagaaca ggcagcagag cgtttgcgcg cagtcagcga tatccatttt
     9241 cgcgaatccg gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac
     9301 gcatttggct accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt
     9361 gaacacaccg actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg
     9421 atcagctgtg caccacgcga tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat
     9481 cagctcgacg agttttccct caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg
     9541 aactacgttc gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggtgtg
     9601 gacatggtga tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc cgcttcactg
     9661 gaagtcgcgg tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa
     9721 atcgcgctta acggtcagga agcagaaaac cagtttgttg gctgtaactg cgggatcatg
     9781 gatcagctaa tttccgcact cggcaagaaa gatcatgcct tgctgattga ctgccgctca
     9841 ctggggacta aagcagtttc catgccgaaa ggtgtggctg tcgtcatcat caacagtaac
     9901 ttcaaacgta ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt
     9961 gcgcgtttct tccagcagcc agctctgcgc gatgtcacca ttgaagagtt caacgctgtt
    10021 gcacatgagc tggacccaat cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc
    10081 cgcaccgttg aagctgccag cgcgctggag caaggcgacc tgaaacgtat gggcgagttg
    10141 atggccgagt ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac
    10201 actctggtag aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc
    10261 ggcggatttg gcggctgtat cgtcgcgctg atcccggaag agctggtgcc tgccgtacag
    10321 caagctgtcg ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt
    10381 aaaccatcac aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggtc
    10441 agccgtaccg actgttaact ttgcgtaaca acgcagggat ggtagtcacg ctgatggact
    10501 ggggtgcgac tttactttcc gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc
    10561 tgctcggctg tgccagtccg gaatgctatc aggatcaggc tgcgtttctg ggggcctcta
    10621 ttggtcgtta tgccaaccgc atcgccaata gtcgctatac ctttgacggt gaaaccgtga
    10681 cgctttcgcc aagccagggc gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac
    10741 gtcgctggca gattgtgaac cagaacgatc gtcaggtgct gtttgccctg agttcagatg
    10801 atggcgatca gggcttcccg ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg
    10861 ataaccgtat ctccattact tatcgcgcca cagttgataa accttgcccg gtgaatatga
    10921 ctaatcacgt ctatttcaat cttgacggcg agcagtctga cgtgcgcaat cacaagttgc
    10981 agattctggc ggacgaatat cttccggttg atgaaggcgg cattccgcac gacggcttga
    11041 aatctgtcgc cggaacgtct tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc
    11101 ttgccgacga cgatcagcgc aaagtgaaag gttacgatca cgcattcttg ttacaggcca
    11161 aaggcgatgg caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga
    11221 aggtctacac caccgctccg gctcttcaat tctactccgg caacttcctc ggcggcacac
    11281 cgtcgcgggg aaccgaacct tacgccgact ggcaagggct ggcgttggaa agcgagtttc
    11341 tgccggacag cccgaaccac cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg
    11401 aagagtattc cagcctgacg gaatatcagt ttattgctga gtaatgttaa gccctcgtca
    11461 tgagggcttt atcttcatat tgttcaaatc accagcaaac accgacatat ttgcaactca
    11521 atattcacaa caaccttaca ctgcgccact attttcgcta tggttatgcg taagcattgc
    11581 tgttgcttcg tcacggcaat ataatgagaa ttattatcat taaaagatga tttgaggagt
    11641 aagcatatgg ctgtaactaa gctggttctg gttcgtcatg gcgaaagcca gtggaacaaa
    11701 gaaaaccgtt tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca
    11761 aaagcagcag gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacttct
    11821 gtgctgaaac gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg
    11881 ctgcccgttg agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg
    11941 aacaaagcgg aaactgctga aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt
    12001 tttgcagtga ctccgccgga actgactaaa gatgatgagc gttatccggg tcacgatccg
    12061 cgttacgcga aactgagcga gaaagaattg ccgctgacgg aaagcctggc gctgaccatt
    12121 gaccgcgtga tcccttactg gaatgaaacc attctgccgc gtatgaagag cggtgagcgc
    12181 gtaatcatcg ccgcacacgg taactccctg cgtgcgctgg tgaaatatct tgataacatg
    12241 agcgaagaag agattcttga gctgaatatc ccgactggcg tgccgctggt gtatgagttc
    12301 gacgagaatt tcaaaccgct gaaacgctat tatctgggta atgctgacga gatcgcggcg
    12361 aaagcagcag ccgttgcgaa ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg
    12421 agaaagccga ctgcaagtga gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat
    12481 tgcctgatgc gacgctgcgc gtcttatcag gcctgtggtg attcatcgga tacgccactc
    12541 tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc ttttactgca ttcaccagtt
    12601 gacgtaacag agcatcggta tcttcccagc caatgcaggc atcggtgatg ctcttaccgt
    12661 aggccagcgg ctccccgcta tcgaggctct gattgccttc caccagatgg ctttccacca
    12721 tcacgccaat aatggccttt tcgccaccgg caatctgctg gcaaacgtca gcacaaacat
    12781 ccatctgctt tttgaattgt ttggacgaat tagcatggct gaaatcgatc atcacctgag
    12841 ctggcagacc tgctttgttc agcccttctt tcacttcagc aacgtgcttc gcgctgtagt
    12901 taggctcttt accgccgcgc agaatgatat ggcaatcgcc gttaccactg gtattcacaa
    12961 tcgccgaatg cccccattta gttacggaca ggaagcagtg cggcgcaccg gcggcattaa
    13021 tggcatcgat agccacttta atcgtaccgt ctgtgccatt tttaaaacct accggacaag
    13081 aaagaccaga cgccagttcg cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc
    13141 cccagctcat caggtcagcg agatactgag gagtgatcat atcgagaaac tcacccgccg
    13201 ctggcagacc gctgtcgtta atatcaagca gcaatttacg ggctatacgc agaccgtcgt
    13261 tgatctggaa gctgttatcc atatgcggat cgttaatcaa ccctttccag cccaccgtgg
    13321 tacgcggctt ttcaaaatag acgcgcatta cgatttccag ctcatctttc agctcttcac
    13381 gcagcgccag caagcgagtg gcatactctt tagccgcgac aggatcatga attgagcacg
    13441 ggccaatcac caccaacagg cgatcatcat tacctttcag gatcttatgg atcgcttttc
    13501 gggcatgggc aaccgtattc gcggcatttt cagttgcggg gaatttttcc agcaatgcga
    13561 caggaggaag taactctttg atttctttga tgcgtaaatc gtcgttctga taattcatgt
    13621 ctgttccagt gttgccatac ttatcttagt gaatgcaata cttccaatct atatcttccg
    13681 tcagaatgtg taaacggggt tttacactat gaacggatta atcctggaat tagcaagaaa
    13741 aacgccagat tgtcgcgaaa aacgagatct ctcctacaat ttctaactgt aactcctttg
    13801 tttgttaatt atttcaagat tctctgctgc gtttcataaa ctggctgaaa tcttaaacca
    13861 atgccttata ttcacctgca aatgcactgt tggaagaggt tatccgacat aacgaccata
    13921 acaggagcat cctatgaaaa tgacaaaact ggccacatta tttctgactg ccactctaag
    13981 ccttgccagc ggtgccgcac tggctgccga tagcggagcg caaactaata acggccaggc
    14041 aaacgccgca gctgatgcgg gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa
    14101 taacgtcgac aataacgggg taaataccgg ttctggcggc acaatgctgc attcggatgg
    14161 ttcttcaatg aacaatgacg gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa
    14221 agatggtcga tgtccggata tcaataaaaa ggtacaaacc ggtgatggca tcaataatga
    14281 tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc aaaatagcca ataatgggag
    14341 agcggagtct ctccctttta atttttcact tctaaaagca gtatgatgtc ataaagttaa
    14401 gtgagaataa ttaagggatg actcgtcatg gcgcactcac actcacacac gtcctcacac
    14461 ctgccagaag ataataatgc tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt
    14521 atgctggtag aagtcgttgg tggttttctt tctggttctc tggcattgct ggccgatgcg
    14581 ggtcatatgt tgaccgatac tgccgccctg cttttcgccc tactcgccgt gcaattttca
    14641 cgtcgtcctc ccaccattcg ccacactttc ggttggctaa gactgaccac gctcgcggcg
    14701 tttgtgaatg ccatcgccct cgtggtaatt accattttga ttgtctggga ggcgatagaa
    14761 cgcttccgca cgccgcgtcc ggtcgaggga ggcatgatga tggcaattgc cgtggctggg
    14821 ttgctggcaa atatactttc tttctggtta cttcatcacg gcagcgaaga gaaaaacctc
    14881 aacgtacgag cagcggcact gcatgtgctc ggggacctgc tgggttcggt tggggctatt
    14941 attgccgcgt tgattattat ctggactggc tggacacctg ctgaccccat tctctcgata
    15001 ctggtgtcgc ttctggttct gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa
    15061 ttacttgaag gtgcaccggt atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg
    15121 gaaatcccgg aagtccgcaa tgtgcaccat gtacatgtat ggatggtagg cgagaagccg
    15181 gtgatgacgt tgcatgtgca ggtgatcccg ccacacgatc acgatgcctt gttggatcag
    15241 atccaacact acctgatgga tcactatcag attgagcaca ccaccattca gatggaatat
    15301 caaccttgtc ataggccaga ctgccacctg aatgaagggg tgtctggcca ttcacatcat
    15361 caccattaat gggacagcgc gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg
    15421 ctgccgttga gcgcaataaa ggtcaggatg atgtactcca gagacatggc gtaaacgccc
    15481 tgaagtgcaa aaataacgac gctaatcacg ttaataatca cccacaacag ccagttttcc
    15541 acatacttac gcgtcatcag aatcattgcc acgatagata acaccatcat gcatgaatcc
    15601 cagaacggga aagcgtccgg ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc
    15661 atgatcatga ctgccacgcg ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc
    15721 agaccaatcg aaacaacgca aaccgccaac cagctgagtg ccttcggcaa tggcaaccag
    15781 cgaattttca actccgcctc gttctgactg gtttgtcgcg accacgcata ccagccgtaa
    15841 atattcgcgg caaagaaaaa cacctgtaat agcaggctgg catacagctg aatctgaaag
    15901 aaaataatgc caaacaaggt aacgttgatc aggccaaaga agtagttgct gatcttctcc
    15961 agactggcaa ggccaataca cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag
    16021 agatcataac cgcccgcccc tattggtata tgtaccagga tattctgcac actaaaaaaa
    16081 tccatctttc ccccaaagcg taacgttacg ccttattatt cgttatccac gtagtgtagc
    16141 cgcaaaatcc agcatacgat tgagcggcac cagcgccctc tctcgcagcc tctcatcgac
    16201 atgaacctcg tgattacttc cttcctgttc taatgcctct gcgatagcct gaaggtcatt
    16261 catggccatc cacggacaat gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg
    16321 tgcttccagt aactctttat ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc
    16381 ggttgccaca ataagcctct gatgaggcaa tgcttttgca gcagcgatca gttgactggt
    16441 ggaaccgacc gcatccgcca tatcgacaat agcttgtggt gattctggat gcaccagtat
    16501 ggcagcatcc gggtattctt cttgcaagcg ggttaacgcc tgagtcttaa attcatcatg
    16561 cacaatgcag gcaccctgcc agcataaaat gtctccaccc gtctgttttt gcacgtaacg
    16621 ccccagatgt ttgtcgggtg cccagatgat tttttcaccc aaactatcaa gatgatcaat
    16681 aagttcgacg gcaatgcttg aagttaccac ccaatctgcg cgcgctttta ccgcagcaga
    16741 agtgttggcg tagacgacga cagtacgatc gggatgggca tcgtaaaatg cgttaaattc
    16801 ttcaacaggg cagccgagat ccagtgaaca ctcaacctga agtgtcggca tcagaattgt
    16861 tttttccgga ctgagaattt tggcggtttc tcccataaat ctcaccccag cgactaacaa
    16921 agtagaagcg ggatgctttg caccgaagcg cgccatttcc agagaatcag aaatacagcc
    16981 accggtttct tctgccagtt gttgaatttc gggatcggta tagtagtggg caaccatcac
    17041 cgcattacgt tcttttagca gacgttttat cttctcgcgg taatacgctt tttcatcaat
    17101 gcttaacggc gtcggcttcg gggggaaagg ataaatcgcc gtgtctggat caaacattac
    17161 gctcatctta ccatctcgtt ttacaggctt aacgttaaaa ccgacattag cgtaacattc
    17221 gctgttatcg gtgtaatttg ttttatatgc taaacaagat agtcgaatgg tggggaaaag
    17281 tcactcgaat tacgcctaat tccaccgacg tagagttgct tgcacagtaa gctctgacga
    17341 tgtcatcagg tttacaaagt gaaattgagg atgcctgaat agcaaaaaag cgcctttagg
    17401 gcgctttttt gcattggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt
    17461 caactgctct accaactgag ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc
    17521 cgaccccctc cttgtaaggg aggtgctctc ccagctgagc taatcacccg atactgcgct
    17581 ggatactaca aactacttcc accgaaccca ccgtaattag tggtgggtcg tgcaggattc
    17641 gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccggtg
    17701 gtgggtgatg acgggatcga accgccgacc ccctccttgt aagggaggtg ctctcccagc
    17761 tgagctaatc acccgacact acgctggata ctacaactaa ctatgttagt gggggtcgtg
    17821 caggatgact cgacttcgtc tcgcccttcg gcgccgttgc tgatgcaacg ttatccttca
    17881 cgttttacta ccgcttccac cttagcgatt ggtgggtcgt gcaggattcg aacctgcgac
    17941 caattgatta aaagtcaact gctctaccaa ctgagctaac gacccacttt ttcgttgctt
    18001 tcggtttgtt tgatatcccg tggcaacggc ggcatatatt actgatttca gacttgagcg
    18061 caacaaaaat ttcgatgcag atcactcaac tgcttatgat tcgcacgaca cgaccagaaa
    18121 taatgcgact tctggtcgtg tgttatgcat tacatcgcgt tcagacgttt ttgtgcctgt
    18181 ttagcgccat cagtaccagg gtatttactg ataacctgct ggtatacggc tttcgctttt
    18241 gcggtgtcac ctttgtcctg catgatgacg ccgactttaa acatcgcatc agcagccttt
    18301 ggtgacttcg gatagttttt cactaccgaa gcaaaatagt acgccgcatc atctttttta
    18361 cccttgttgt agtttaactg acccagccaa taattggcgt ttggcaggta agttgaatca
    18421 gggtaatttt tgatgaaatt ctgaaatgcc accattgcgt catcctggcg ggatttatcc
    18481 tgcaccagcg caatagctgc attgtaatcc gtgtttgcat cgccgctttt caccggcgcg
    18541 ccagcattcg cagtaccagc atcagctgtc ggcgtcgttg atgccgtcgc accgctttga
    18601 tcgctgctgg ttgattgcgc cgctgcacca ccgctgctga ggctgtcgat ctgcaacagg
    18661 atctgctttt gccgctccac gacctgattc agttgatact gattttcctg aatctgacca
    18721 cgcagggaat caatatcgga ttgattatca gaaagttgtt gctggagttg ggttaaaagc
    18781 tggctgtgag cattagaaat acgctcaagt tgagtgacgc ggtcttcgac cgagcctgag
    18841 ctgacactac tgattggtgc ctgagcaaaa gcggcccagg gggccgctat accaaccagt
    18901 aacgacagac tcaatagttg atgtctgaag ttactgctca tgcaattctc ttagtaaacc
    18961 agtaccgcac gacggttttt ggagtatgcc gcttcgtcat gacccagtac tgcaggtttt
    19021 tctttaccgt aagaaacgat ggagatctgg tctgcagaaa cgcctttacc ctgcaggtac
    19081 atcttaacgg cgttcgcacg acgttcaccc agggagatgt tgtattccgg agtaccacgt
    19141 tcgtccgcgt gaccttctac ggtgactttg taagacgggt tgctacgcag gaagtttgca
    19201 tgtgcatcca gcatttgagc gaagtcagaa cggatatcgt acttgtccag atcgaagtaa
    19261 acgatgttgt tctgctgcag ctgttgcatt tgcagacgag cctgctcttc ggaagacatg
    19321 ttgccgttgc cgccgttcgc atccatacca gtgccggcac ccagcatgcc ttcgctgccg
    19381 tcattgctgg cgttcttgtt ggaagaacat gccgcaattg ccataacagg cagagcaatc
    19441 atcagccctt tcagcacttt gttcagttgc atttcaatga ttcctttact attcaattaa
    19501 ttattatcac agatacggcg accaggcagg gaatttgacc tgtccatcag ttgccggaag
    19561 acgcgctttg aaacgcccat ctgtagaaac caaattcagc acggatccca tcccctgaga
    19621 agagctgtag attaccatag tgccgttagg tgccagactt ggcgtttcat ccaggaacgt
    19681 ggacgacaga acttgtacgc ctcccgttgc cagatcttgt ttggcaatgt gctgctgccc
    19741 accattggag ctgaccatta ccataaattt accgtcgctg ctgacatccg catcctggtt
    19801 ctgcgaacct tcccaggtaa tacgttgtgg cgcaccgccg ttgatattca ctttataaac
    19861 ctgcggacga ccggcctggt cagaagtaaa tgccaggttc tggctatccg ggaaccaggt
    19921 cggttcggta ttgttactgc gaccatcagt cacctggcgg atctgaccag aagccaaatc
    19981 cattacgtac aggttcagac taccggtttt cgacaaggcg aatgccagtt tgctgccgtc
    20041 tggcgagaat gcaggtgcac cgttgtgacg cgggaatgaa gccacctgac gtacagcgcc
    20101 atttgccagc gtctgaataa ccagcgcgga acgaccgctt tcgaaggtca cataagccag
    20161 tttagaaccg tctggtgacc acgccggaga catcagcggc tgcggtgaac ggtgaacgac
    20221 aaactggttg taaccgtcat agtcagatac gcgcagttca tacgggaact gaccgccgtt
    20281 ggtctgaaca acgtaggcaa tacgggtacg gaacgcacct ttaatgccgg tcagtttttc
    20341 aaacacttca tcactggcgg tatgaccagc ataacgcagc cactgcttgt tcactttgta
    20401 cgagttctga gcaagtacag tacccggtgc gccgccagtg tcaacaagtt gataagcaac
    20461 attgtaagaa ccatccggat tcggagtgac ttgaccgaca actacagcgt caatgcccag
    20521 tgcggaccat gcagctggtt gtacttcctg cgcactaccc ggctgctgtg gcagacgagc
    20581 gcgatctaac ggattaaatt taccgctgtt acgcaagtca gcagcaacga tgccgccaat
    20641 atcttcaggt gccgcaccag gccccgccca ctggaaagga acaacaccaa taggacgacc
    20701 ggaatctaca ccgctgtcga tcacaatgcg gacttcagca tgcagaactg atgcccacag
    20761 tatgagaaaa ccaaatgcta ctcgtaatgc ctgcttcatc atatctccct tatctggacc
    20821 gatggcccac gataatttag cagaatgtta acaaactaaa atacacaaaa ctaccagaac
    20881 cccgtggcaa ccggtgcctg atgttgaccg tccgaacagt caacatcgcg attacggttt
    20941 gaagtccaat ggcgcgtttt tgaacacttc atatactgcc tggcttggtg gtttcgggat
    21001 cttcgcaagt ttagctgctg ccaacgcagc ctgacaaagt gcgggatcgc caccttcagg
    21061 tttgatatcc agtaacatac catcgggtgc cagttttatg cgcagcgtac aggttttgcc
    21121 tgcataggac gatgcgtcat agaacttact ttcgatagca gatttaatct gcccggcata
    21181 gttattgata tcggcccctg atgcgccatt gtttttagta ttaccactcc cggcaggcga
    21241 agcattgttc cctttcgccc ctccccccgt tttcggtgca ttcttaccag agcttagctc
    21301 accgaaaata tcatctgcct ctgcggcagc ttttgctgca gcggcttttt cagctgctgc
    21361 ttttgccgct gccgcttttt tgtctgcggc ggctttttct gccgctgctt tcttatcagc
    21421 tgctgcctta tcagcagccg ctttttctgc tgcttttttg tcggctgcag ctttctctgc
    21481 tgccgctttc ttatcagctg cagccttttc agcagccgct ttcttctcag cttctgcctt
    21541 ggctttttca gcagcttcag ttgccgcttt ctttcttgct tcagctgcag ctgcttctgc
    21601 cgcttccgct ttcttcttca gtgccgcagc ggctacctcg gcttttttct gcgcttcggc
    21661 tgcggctttg gcggcttctg cttctgcttt tttcttcgcg tctgcagccg ctttcttcgc
    21721 tgcttcttcc gcagctttag catctgccgc cgctttcgct gccgccactt cagcttgctt
    21781 ctgctttaac tcggcctgtt ttgcggcttc ttcagcctgc tttttctgct cctgagccgc
    21841 taaccgctct ttctcaagtt gcttcaggcg ttcctgttca gccgcttgtt tctcacgcag
    21901 ttcttcagca gcctgctgtt ccttcatctt gcgctgttca tcagaacgct tcgcgcttga
    21961 ttcctggctt tgcatgcgtt tgtactgctc aactaccgca cctgaatcaa ccatgacagc
    22021 gtcgatggac gaaccaccgc cgcctccggc tgaagcttct atattctcat cgaacgaact
    22081 ccagatcagc gccgcaaata agatgacatg cagcactgct gaaataatta tcgcccgctt
    22141 gagcttgtcg ttttgttcgg ttgcctttga cactctcggt ttccaaaaac tgttcgcctg
    22201 ttacccgctc tctttcaagc aagggaaacg cagatgttta gataggctgc gtcattaaac
    22261 caaccgattt cacacccgca ctatgtaaca agttcagtgc tttaattatt tcatcgtaag
    22321 gcacatcttt tgcgccaccg atcagaaaga ccgttttcgg gttggccttg aaacggctgg
    22381 acacttccgc caccacctgc tctggtggta aacgctccag gcgatctttc tcaaccacca
    22441 cggtgtactg accaatacca gacacttcaa caatcactgg cggattatcg ttactgctca
    22501 ccgcctgtga ttcagtagcg tctggcagat cgacctccac gctctgggtg atgatgggcg
    22561 ctgtcgccat aaagatcagc aacagcacca gcagtacgtc cagcaacgga acaatgttga
    22621 tttcggactt gagatcgcga cgacctcgtc cacgcgctct ggccatggct taccccttgt
    22681 tgctctcgct aacggtaaac gcctggcggt gcagaatcgc ggtaaactct tccataaagt
    22741 tgtcgtaatt cagttccagt ttgtttacgc gctggttgag acggttataa gccattaccg
    22801 ccggaattgc ggcgaacaga ccaattgccg tcgcaatcaa cgcttctgcg ataccgggcg
    22861 caaccatttg cagtgttgct tgttttaccg ccccgagggc gataaaggcg tgcatgatcc
    22921 cccagaccgt accaaacaga ccaatatacg ggctgatgga gccaaccgta ccgaggaacg
    22981 gaatgtgcgt ttccagattt tcaagttcac ggttcatgga gatacgcata gcacgcgacg
    23041 ccccttccac tacggcttcc ggcgcatggc tattggcacg atgcaggcgc acaaactctt
    23101 tgaacccgct gtaaaagatt tgttccgaac cagtcagatt atcccgtttc ccctgactct
    23161 cttgatagag gcgagagagt tcgattccag accagaattt atcttcaaac gcttcggctt
    23221 cacgcgccgc tgcgttaaga atacgggttc gctggataat aatggcccag gatgcgattg
    23281 aaaaaccaat caaaatcaac atgataagtt taaccagaag gctagccttc aggaacaaat
    23341 caaggatatt catgtcagtc actgcttaaa ctccgcgaca atagacttgg gaagcgcacg
    23401 aggcttcatt ttgagtgggt caacgcaaac aaccagaacc tctgcttcat tcagcaaagt
    23461 attctcggcg ttgacaatac gttgcgtgaa aaccaaagag gtgccacgca ttgatgttat
    23521 ttcagtctgt atttcgagca tatcgtcgag ccgcgcaggt gcgtaatatt ccaccgtcat
    23581 tttacgtacc acaaaggcaa cgcgttcagc catcagcgcc tgctgactga agtgatgatg
    23641 acgcagcatc tctgtgcgtg ctctttcata aaaagcgacg taactggcgt ggtacaccac
    23701 accaccggca tcggtatctt catagtagac gcgaaccggc catcgaaaca gcgttgtatt
    23761 cactttacat cccggtaatg caacaaaagt tagagctttt aaacgctgct actatacgcg
    23821 cgcgattgag gtttgggaat gagggcaagt taagggcgcg taaaaattta tgggcctaga
    23881 gcggcccata attttttaaa gagaattctg atttaaaaga agaagaaaat cagcccgaca
    23941 atgagaacaa tatcggcaag caatgggcaa aaaatccctt gccaaagaac cttctgcgga
    24001 cgaaagccca cgccgtgaat cacaccagca cagacggccc acatcagcaa taaaccatgc
    24061 cagatttcca gttcactggt cttcgcggca aaacgagatg ggtcccaaaa catacatcct
    24121 gctaacagaa gcgccatcac gaaggaaagc gcccgcaggg ggcgcttgtc cattaccgca
    24181 tacaaggtcg cgataatctt actcatcaga tgtcttcttg accggctttg cctgattcga
    24241 cgtgttcaag cgccagcgcg gtgattaccc caaacgaaca ggcaagaagc gttcccagaa
    24301 tccatgcgaa ataccacatt tttagctcct tacttagtac agagagtggg tgttacgttc
    24361 aatatcttct ttggtgatac gaccgaacat tttccagtaa caccaggcgg tgtagagcag
    24421 aatgatcggt accagaacca ccgcaaccca ggtcatgacg ttaagcgtca gctggctgga
    24481 agttgcatcc cacattgtca gacttgcgtt catcatggtg ctggacggca tcacaaacgg
    24541 gaacattgcg ataccggctg tcaggatgat gcaggccagc gtcagggagg agaacacaaa
    24601 cgcccacgcg gctttatcca tacgtgcagt caggatggtc agcagcagca gaaccacacc
    24661 cagtgccgga atagcccaca gaattggcgt gttgttgaag ttaaccagcc atgcgccagc
    24721 ttcacgaacc acttctttat tcagtgggtt agaggctgcg taatggtcca ttgtcgattt
    24781 cacgacataa ccatcgatac cgtacatcac ccatacgcca gccagtgcga aacagaccag
    24841 tgtcaccagc gcagccacct gagccgttgc acgggtacgc aggtgcagtt cgcccacggt
    24901 acgcatttgc agataggttg cgccctgagt aatgatcatc cctacgctca ccacgcctgc
    24961 cagcaggccg aacgggttaa gcaactggaa gaagttaccg gtgtagtaca gacgcagata
    25021 ttcatcaacg ttgaacggta cgccctgcaa caggttaccg aacgctacac caattaccag
    25081 cggcggaacg aagctaccaa tgaagatgcc ccagtcccac atgttacgcc agcgggtttc
    25141 ttcaatcttg gagcggtagt caaaaccgac cggacggaag aacaaagacg ccagcacgag
    25201 gatcatcgcc acatagaagc cggagaacgc agcggcatag accatcggcc aggcagcaaa
    25261 gagtgcgccg cccgcggtga tcagccaaac ctggttaccg tcccagtgtg gtgcaatgga
    25321 gttaatcata attcgacgct cggtgtcgtt acgaccgagg aaacgggtga gcatgcccac
    25381 ccccatgtcg aaaccgtcag tgactgcaaa accaatcagc agaacgccaa ccagcagcca
    25441 ccagataaaa cgcaatactt cataatcgat catttgacga ctcctgtctt agcgtgccgg
    25501 ctgagtagtc gtggaagact gctcaaagtg atagcgaccg gttttcaggc tgcttgggcc
    25561 gaggcgtgca aacttgaaca ttaagaacaa ttctgccacc aggaacaggg tatacaggcc
    25621 gcaaatcagc accattgaga agatgagatc gcctgcggtc agtgacgaat tcgccacagc
    25681 tgtcggcagc acttcaccga tagcccacgg ttggcggcca tattcagcca cgaaccagcc
    25741 cgcttctaca gcaatccacg gcagcggaat accgtacagc gcggcgcgca gaagccattt
    25801 tttctcgcca atgcggttgc ggatgacact ccagaaagag agcgcgatga ttgccagcag
    25861 caggaagcca cacgccacca tgatacggaa cgcaaagtac agcggcgcta cacgcgggat
    25921 ggagtctttg gttgcctgtt gaatctgcgc ttcagtcgca tcagccacgt ttggcgtata
    25981 gcgtttcagc agcagaccgt aaccgaggtc tttcttcatg ctattgaact ggtcacgaac
    26041 cgtctggtcg gtagaaccag aacgcagttg ttcgagcaga gagtacgcct tcatcccgtt
    26101 acgaatgcgt tcttcatgct gcaccatcag ctctttcagg ccgataaccg gggtatccac
    26161 ggaacgcgtt gcaatgatgc ccagtgcgta agggatctga atcgcaaatt tgttcgtctc
    26221 ctcttcctga tcaggaatgc cgaacaaagt aaaggcagca ggcgcaggtt gcgtttccca
    26281 ctcggcttca atagcagcca gtttggtttt ctgcacgtcg cccatttcgt agccggattc
    26341 atcacccaga acaataacag acagaacagc agccataccg aagctggcag cgatagcaaa
    26401 ggagcgttta gcgaaggcga agtcacgacc tttcagcata taccatgcgc tgataccgag
    26461 gatgaacatc gcgccagtca cataaccaga cgctacagtg tgaacgaatt tcacctgagc
    26521 aaccgggtta agcaccagct cggagaagct caccatctcc atacgcatag tttcaaagtt
    26581 gaaatcggac gcgattgggt tttgcatcca gccgttcgca accagaatcc acagtgcgga
    26641 caggtttgaa ccgagcgcca ccagccaggt gacacacata tgctgaactt tacccagacg
    26701 atcccaaccg aagaagaaca gacctacaaa ggtggattcg aggaagaagg ccatcagacc
    26761 ttcgattgcc agcggcgcac cgaagatatc ccctacatag tgggaatagt aagaccagtt
    26821 agtcccgaac tggaactcca tggtcagacc ggtagccaca cccagagcga agttgatacc
    26881 aaacaacttg ccccagaact tggtcatatc tttataaatc tgtttgccgg agaggacgta
    26941 gaccgtttcc ataatggcca ggaggaacgc cataccgagc gtcagtggca caaaaaggaa
    27001 gtggtacatc gcggtcaagg caaactgtaa gcgcgacagt tcgactatat ctaacatcat
    27061 gactccttgc tcatcgcatg aagactccga gagtgaaccc cgttagaaag ggtcacacgc
    27121 atgccccaat agaaatttgt ttgaacactt tcatcatacc atgtgaatgc acacaaaaac
    27181 ggcgtaaaga aaggttgcaa acatgttaat aaaaactcaa attgatccca cgtatatatt
    27241 acgccgcaaa atccttacaa taaacaggtt tttgttgaag caaatttgca tttttcgacg
    27301 gtgatcaatt tatagctaaa ttgccgcctt tcagccaatt tgatcgagaa caatttatct
    27361 ctttttgatg cccatttcca agacttatac attgataaat atcaatttct acaaattatg
    27421 ttaatcatgt ttcaacaagg taactttagt gatatttatg tttaaaatgt gtgaattata
    27481 agactgaaat tagtttcggt tggcaattaa agataaaaag agacgaagca aaaacagcac
    27541 ggttgacctt aattcttttt ttggtcactg gctaatacga cgcagagttt caggattatt
    27601 aataaaggca taaatctccc ccccgccgat attaacattt aattaactta taattatatt
    27661 ataattaact cacccgtggt tattattttg ttaaaaataa agccacatat acgtagcttg
    27721 agagtgaaga taaacacaaa attacctgat gcgctacgct tgccagtgtc tacatcatct
    27781 ctgcaatata ttgaatttgc ctgcttttgt agaccgaata aagcgttcat gccgtatccg
    27841 gcatgaacaa agcgcccttt gtcaacaatc tggccgcgca tatgtggctt cggtttgatt
    27901 gctattcagg caagccggta actgaacgtc cgtgactggc cgggtaaaaa aggccccgat
    27961 agatttgaac cttgattttc ctcggtggta atgtgttcat ccatcatcgt ttctgagcaa
    28021 gaaatcacct cgcgactgaa agcaacagtc gcatcacagg ttgctgattc agccggatta
    28081 aacagccgta aaatcacttc ttgtcggtct tcagctttct taagtgcgct tatcaggcat
    28141 cccactgggg gcattttcaa caaactataa ctttccggca cgttgaatcc ggctttgttg
    28201 agcttcatcg catcccatgg gattttgttg taacactgta ctggagtcag ccatgctcgc
    28261 gcctgctgag ctacaccagc ggcggttggc gtaccggtat aactcaataa acttaggcga
    28321 caagaaagca gaccacgtag ttgtgagtcc gggactggca ttttaattcc cgaaggccgc
    28381 ctaggcctta aaagcagatc ttctttcccc agtaagccca cgccgcgcag caacgtaatg
    28441 gcaaaggttt tcttctcttc accgatgact tcaaattcac gtagcccttc gctaaagaca
    28501 gccatgccgt tacgcccttc ctgtaaggct gcatagttga gcatattcca taccggaacc
    28561 ggcgcttctt tccagctttc ttgctgccag ttgttcattg cactgtcgtt cacggggcgc
    28621 gttagcgaac caaactgggt atctgccaga acactatcgg tgttaaaaga tgtagggatc
    28681 aggacgcgaa ggcgatgatc gtcagcctgg ttatcaaggt tgatatccac atcaatgcgc
    28741 ctgttgttat gactaagagt gacaaccaac tcgacatcta ctctgccagt ggattgtctt
    28801 gcgctgcgtt gttgcagatt acgcggcacc gccatttcat agcggataac agccctgctc
    28861 tgccaggcct catgaataat ctcgcattgc ggtttcgcat tcgccgaagt aattacccac
    28921 tcttcttttg cgggtgaata gtcatattca tcaccatcat cagagctttc ttcaatttgc
    28981 aacacccggt catagcgcac accgctatct ttatctacca gttgtagcgt tccatcctca
    29041 ttgagcgcaa tttgccagaa agcattttcc agtatccctt cagcgtcact ttttgccgca
    29101 atcacgttgc caggctgatt cgcttcgata taaagcgtgc gatagcccat agaagggaca
    29161 atctggttga tctgtatatc aaactccata aagggatcgt aattaccgta atgaactatt
    29221 tgccgatcga ttaggcctgg atcgatctca cgggcatggc gaataaaata cggtacaggc
    29281 tgaccgcgat cgtctcgtag attaaactgg ctggcgcgca gccgcacagt ggtgttgatc
    29341 acttcttccc gcggccaggg catcaggtta aacaggacga gtttgtcggc gtcgctctgc
    29401 ggcatgttgt cggcgatttt acgcatatag aaacgcagca gattatccgc catgtcttca
    29461 gccagttcga agcgggcgac gatttcgcga tgaactttgt cacgacagca gcagccgatg
    29521 ctgtcgtggg catgattttt taagatctct ttccacattt tctccagcaa gccgtggtgg
    29581 tatccaaaac ccaacgtcct ggccagcgtt gccagcggtt ccagcagatt aacaatctta
    29641 ttttcaatac gtgcgtgggc aactttgata tccatgcgcg tggaaccgat ggtgcgatgc
    29701 acgcgcatat atttgccatc aataaattcc cctttcaggg ttgccagatt atctcgctgc
    29761 gcttcgatct tctcaaatac ctcttcaaag cggctcatca caaactttcg ttgaggataa
    29821 atttcgcgca gtttctccat cacttcgaag atattttgct gcaatggcat ctgatcatgc
    29881 ccattcggca gcaaaatctc tttggttaca gacgcttttt ccagcacgtc aaaataactg
    29941 tcgaggcgtt tgcgtaatcc gttttcgtcc gcaggtaagt acttaccgat ggcataaccc
    30001 aacggcatca cctgcgccgt cacttcgcta ccgtcactgc tttgccacaa aaactcggtt
    30061 ttatccgtac cgtggcgctc agaacatcca cgccagaaca tggtgcaggt aatgccaaat
    30121 ccattgtaga tatgcggcag ttgcccggac atgccaaagg aatcgggtaa ataacctatt
    30181 ttcatcggct cgccaaacgc gaggcagtca cgcattccgt acatcagatt acggacgatg
    30241 gattccgcag aaacaatcgt ggtatcggtc tgggtatacc aggggccgat aatcaacttg
    30301 ccggcttcta ccagtttctt caagcggtct ttgttttccg gtttcaccgc gaaataatct
    30361 tcgaggatcg ccgtttgccc gtcgagtacg taatatttgt attcgttgtc ctgttccagt
    30421 cggcacagga tctcttccat attattgacc agcagaatgc gtgactcttc ggtggtgaaa
    30481 taccactccc gatcccagtg catatgcggg gtgatgtgaa cgcgagatac cgctttcatc
    30541 gtcgtttcct ttttgtttat ggcattacgc catcagtcag atagttgcca tgcttaaccg
    30601 cgtgacgccg ccagatcagg agaattgcag tcgagattgc agcccccacc aatgccgcac
    30661 caaaccagcc aattgccgcc ataacaccgc ccgcgccatt atcatgaagt aaaaagagcg
    30721 agaaaatgcc agcaccgggt gtcgaaaggc cgatattcat cgcaccgaca atagcgcccg
    30781 ttaccataga gcccagcaca aacgaaccaa taacccgcag cggatcttca atcgccatcg
    30841 ggatcgcccc ttcggtaata cctgccagcc ctaacagcca ggtggatttc ccggtctcaa
    30901 tttcaaactc tttaaacagg cgcggtgcga gcatcgtgga agcggttacg gtaaatgccg
    30961 aaaccatttt gacggaggcg aaaatggcat acgggccgta aacgccgttc gccattgcgc
    31021 ccaggcagaa tgcataagcg gctttattca ctggccctcc aaggtcaaag gaacacataa
    31081 aaccgagaat ggcacccagc aacagcgcgt tacttcctga cagaccgttc agccaggcgg
    31141 taagcgagtt attgatccag gcgacaggtt cccccaccac aaacagcatc agactgcccg
    31201 ctcccaatgt accgagcacc gggtagagat aaaaagtcag gaagccattg aatttactgc
    31261 taagacgcaa gtgatttttc atccagcgta tcaagtaacc ggctatcaat ccgccaacga
    31321 ccgcgccgag aaacccggag ccgatcatgt tggcggcaag tccagccgca aagcctggcg
    31381 ctaacgccgg tttatctgcc agcgaatagg cggtataggc tgccagcacc ggtaccatca
    31441 aaattccgag cagcccgccg cccagtttgc ggtacatcca cagccaggag ttttcttcat
    31501 taaacagatc ttgtagcccg aagatttgcg acagtaatac cgcgaccgcc agcaccgtcc
    31561 cccccgcgac aatcaacggt acggcaaaag agatcccgct caacagtgcc tgtttcagct
    31621 ccgttttgac gcttttcacc ggttgcgtat cttgctgtaa ggtacgcgtc tcatcgccac
    31681 gctcgagggt aagcgattgt tggatcaacg cttctgcatg gcgaatcggc tcggcaacag
    31741 gcacagaaag cgcaggaatg ccgttaaaac gctcactctc cttgatggcg acttcagccg
    31801 caaaaataca ggcagttgca ctattgagtt gatccgccgt taaacgccct tcaataccgt
    31861 tagcaccttg cttttcaacg tagacattca cgccgagttt gcgtccggct ttttccagat
    31921 actccgcagc catgtaggtg tgagcaatac ccgccgggca ggccgttacg caaacgatag
    31981 ttggcgcgtt ggaaaaagag gcagaaggtt gcgtgcctcc cttgtcatcc agcgccgaga
    32041 gcaattcatc aggcgtcgtc gccgactgta tacgcgcccg aatctcatca tccgccaggc
    32101 gcgtggtcag cgctgtcagc agttgcatgt gcgtagtacc cgcttcattg aggggaatcg
    32161 ccagcaacac cactaaatca accgcttccg ggccatccac gccttcccac tgaagcggct
    32221 cgctgagtgt ggcgaccgca aacgccgctt cttttaccgc agcagttttg ccatgcggca
    32281 cagccaaccc ttcacttaag gcggtcgggc caaggctttc acggcgatac acttctttca
    32341 gaaattgctc agtactggaa attttcccca gagcagcaag acgttgagtc aacgcgtgaa
    32401 tggcctcttc acggctggta aagcgcgcat tcagacacaa cgcatcgcgg tgggtcagag
    32461 tcgtcaggtt catcatgatc ctcacttgat cgtttttata gtcgcgccag tgtgcccgac
    32521 aaaacaatac cattgcgtaa tcaatttcac ttgattgaaa ttatttgcat tttatttgta
    32581 ttgtttacag gatgagttta tcgggcataa tggctaaatt cgtattaatg agatgctaaa
    32641 taatgggaca caaaccctta taccgacaga ttgctgatcg cattcgtgag caaattgcgc
    32701 gtggcgagct taaacctggc gatgcgttac ctaccgaatc ggcattacaa acagagtttg
    32761 gcgtcagccg ggttacggtg cgtcaggctt tgcgccagtt agtcgagcaa cagatcctcg
    32821 aaagcattca gggcagcggg acttacgtca aagaagagcg ggtcaattac gatatttttc
    32881 agttaaccag ttttgatgaa aaactgtcgg accgtcacgt cgatacacac agtgaagttc
    32941 tgatatttga agtgattccg gctgacgatt ttcttcagca acagctacaa atcaccgtgc
    33001 aggatcgtgt ttggcatgtg aagcgcgtgc gctatcgcaa gcaaaagcca atggcgctgg
    33061 aagaaacctg gatgccgctt gcgttgttcc cggatctcac ctggcaggtc atggaaaatt
    33121 cgaaatatca ctttattgaa gaagtgaaga agatggttat cgatcgtagc gaacaggaaa
    33181 tcattcctct gatgccaacc gaagagatga gccgtctgct taatatcagc cagacaaagc
    33241 cgattcttga aaaagtatcg cgtggatatt tagttgatgg tcgggtgttt gaatatagcc
    33301 gcaacgcttt taataccgat gactataaat ttaccctgat agcgcagcga aaatcatcgc
    33361 gataagcaca aaaaggccat catacgatgg ccaaccatgt cgaaaccgac agcccttcgc
    33421 cattcctgta gagggaagcg gcgagggcta tttcttatta cagatattta tttcagaaca
    33481 gttttcagtg cttcaccgat atccgccagg ctgcgaacgg ttttcacgcc tgcggcttcc
    33541 agagcagcga atttctcatc cgcagtccct ttcccaccgg caatgatcgc acccgcgtgg
    33601 cccatacgtt tgcctttcgg cgcagtcaca ccagcgatgt aacccacaac tggcttggta
    33661 acatgctctt tgatgtacgc tgccgcttct tcttcagcgc taccgccgat ctcaccgatc
    33721 atcacgatcg cttcggtctg cggatctttt tcgaacattt cgagaatgtc gataaagtta
    33781 gagccaggga tcgggtcacc gccgataccg acacaggtcg actgaccgaa accgtaatcc
    33841 gtggtctgtt taaccgcttc ataggtcagt gtaccggaac gggaaacgat acccacttta
    33901 cccggtttgt gaatgtgacc cggctggata ccaattttgc attcacccgg ggtgataacg
    33961 cctgggcagt tcgggccgat catacgaacg cctgcttcgt ccagtttcac tttcacggtc
    34021 agcatatcca gcgtcgggat gccttcagtg atggtgataa tcagtttgat gcctgcatcg
    34081 atagcttcca gaatggagtc tttgcagaac ggtgctggta cgtaaataac agacgcggaa
    34141 gcgccagtag cagcaacagc ttcacgcacg gtgttgaaca ccggcaggcc gaggtgggta
    34201 gtgccgcctt tacctggagt tacgccacca accattttag tgccgtatgc aatggcctgt
    34261 tctgagtgga aagtcccctg gctaccggta aagccctggc agataacctt ggtgttttta
    34321 tcaattaaaa tggacattat ttcccctcca ctgcggcaac aacctgctga gctgcatccg
    34381 tcagaccttt tgctgcaata atattcaggc cgctgtcagc cagtttcttc gcgccgagtt
    34441 cggcgttgtt accttccaga cgtaccacga ccggtacgtt aacacccact tccgctaccg
    34501 cgccgatgat accgtcagcg atcaggtcgc aacgaacgat accgccgaag atgttaacca
    34561 gaacggcttt cactttgtcg tcagagagga tgattttgaa cgcttcggtt acacgttctt
    34621 tggttgcgcc gccgccaacg tccaggaagt tagccggttc gccgccgtgc agtttaacta
    34681 tgtccatcgt acccatcgcc agacctgcgc cgttaaccat acaaccgatg ttaccgtcca
    34741 gcgcaacgta gttcagttcc cactgtgcag cctgtgcttc acgcggatct tcctgcgact
    34801 ggtcacgcat ttcgcgcaga tcaggctggc ggaacagggc gttgccatca gcgcccagtt
    34861 tgccgtcgag gcaaatcaga tcgccctgtt tggtgatgac cagcgggttg atttcgatca
    34921 gcgccaggtc gcgctccagg aaaatggtcg ccaggcccat gaagattttg gtgaactgct
    34981 gaaccagttt accttccaga cccagtttga acgccagctc gcgtccctga tacggcatcg
    35041 ggccagtcag cggatcaagc gcaactttat ggatcaggtg cggagtttct tccgccactt
    35101 tttcgatttc cacgccgcct tcggtggagg ccataaagac cacgcgacgg gaactacggt
    35161 ctacaacggc accgagatac agctctttag cgatatcggt cgccgcttca accagaatct
    35221 ggttaaccgg ttggccattg gcatctgttt gatacgttac cagacgcttg cccagccagt
    35281 tttctgcaaa agcacggatg tcttctttgc tgtttacaac tttcacaccg cccgctttac
    35341 cgcggccacc agcgtgaacc tgacatttca ctacccacgg accggcaccg atttttgaag
    35401 cggcttcttc tgcttcgcgc ggagtagtac aggcataacc caccggtgct ggtaagccat
    35461 agcgggcaaa cagttgtttt gcctgatatt catgtaagtt catgtgttct gtccatcctt
    35521 cagtaatcgt tatcttttaa accgtagacc gacaccctat ccacgtcggt ctacagtgca
    35581 ggtgaagctt aaactactac acgtccagca gcagacgcgt cggctcttcc agtaactctt
    35641 tgatcgttac caggaagccc acggattcgc gaccatcgat cagacggtga tcgtaggaca
    35701 gcgccaggta catcatcggc aggatctcaa cctgaccatt caccgccatc ggacgatctt
    35761 tgatagcgtg catacccaga attgcgctct gcggcgggtt gatgatcggc gtagacatca
    35821 gggaaccgaa cacaccaccg ttggtgatgg tgaagttacc accggtcaga tcttcaaccg
    35881 tcagcttgcc atcacggcct ttgactgcca gctctttgat tttcttctcg atgtctgcca
    35941 tgccgagggt atcgacatca cgcagaaccg gcgtcaccag gccgcgcggc gtagaaaccg
    36001 ccatgctgac gtcgaaatag ttgtggtaaa ccacgtcatc gccgtcgata gacgcgttca
    36061 cttccgggta acgtttcagg gcttcaacca ccgctttcac gtagaaggac ataaagccca
    36121 gacggatgcc gtggcgtttt tcaaacgctt caccgtactg cttacgcaga tccataatcg
    36181 gcttcatgtt gacttcgttg aacgtggtca gcatggcggt ggagtttttc gcttccagca
    36241 gacgctctgc cacacgctta cgcaggcgag tcatcgggac acgtttttca ctacgtgcag
    36301 ccagagccgg ttgcgccgcc ggagcagccg ctgccggagc agactctttc gccggggctt
    36361 tcgccagatg tttttccaca tcttcacgag tcagacgacc acccacaccg gtgcctttaa
    36421 tggcgctggc gtcgagattg tgttcagcca gcaggcgacg gatcgccggg cttaacgcat
    36481 cgttgttttg ctcttccaga gacgcctgct ggcgttgcgc cggagtggac gctttctctt
    36541 cagatttggc gctggtttct ttaccggcgc tgttgccttc acgcaggcga ccaaggatct
    36601 gacgagacgt taccgttgta ccttcatctt ccagaaccgc atccagaatg ccgtctgctg
    36661 atgccggtac ttccagtacc actttgtcag tttcgatttc taccagcact tcatcacgta
    36721 cgactgcgtc gccgggtttt ttatgccagg ttgcgacggt ggcatcggct acggattcag
    36781 gcaggtcagg gaccagaata tctacgctac tcattgtgta tcctttattt attcgacgtt
    36841 cagcgcgtca ttaaccagat cttgttgctg tttctggtga acggacatat accctaccgc
    36901 cggagaggcg gaggccgggc ggcctgcata acgcagagaa gccccaaacg gaatcacttc
    36961 acggaaatga tgctggctgc agtaccatgc gccctggttg agcggctctt cctggcacca
    37021 gacaaaatcc ttgacgtgag caaactgctg caacacttcc tgcatcgctt tatgcgggaa
    37081 cgggtagagt tgctcgatac gcacaatggc gacatcgtgt tgattgttct tacgacgctg
    37141 ttccagcagg tcgtaataaa ccttaccaga acacatcact acgcgcttca cgcccttcgg
    37201 atcaagctcg tcgatttcac cgatggctgg caggaaggtg ccgttcgcca gttcttcgag
    37261 gctggaaacc gccagcggat gacgcagcag ggatttcggc gacatcacga ccagcggacg
    37321 acgcatcccg cgcagcgcct gacgacgcag catgtggtaa acctgtgccg gggtagacgg
    37381 tacgcaaacc tgcatgtttt gctcagcaca aagttgcaga taacgttcca gacgcgcgga
    37441 ggagtgctcc ggcccctgcc cttcgtaacc gtgcggcagc aacatcacca gaccacacat
    37501 ccggccccat ttctgctcgc cagaggagat gaactggtcg ataaccacct gcgcaccgtt
    37561 ggcgaagtca ccgaactgcg cttcccagat ggtcagggtg cgtggttctg cggtggcata
    37621 accatattca aacgccagca cggcttcttc tgacagtacg gagtcccaga cacggaacgc
    37681 gccctgaccg ttatggatgt gttgcagcgg cgtgtaagtg gaaccgttag actggttgtg
    37741 gatcaccgcg tggcggtgga agaaggtacc gcgaccggag tcttcacccg acaggcgaac
    37801 cggaatgcct tcatcaacca gcgtggcgta agcgaggttt tccgcaccgc cccagtcgaa
    37861 cagtttctca ccggctgcca tcgcctggcg atcgccataa atcttggcaa cgcgagactg
    37921 catttcaact gcttctggta ccgtactgat acgtttagcc agttcctgca ggcgcttcat
    37981 ctcaactttg ttcgggtact cttcgtccca ttcgtggttg aggtacggcg accaggtgaa
    38041 agagtgcatg ttcatcggac gccactctgc cactacgcaa tcgccagcat ccagcgcatc
    38101 gcggtacagg ttaaccatct cggtggcatc ttccagcgtc gccacttttt cctgctccag
    38161 cttgtcagcg tagattttgc gcggtgtcgg atgttttttg attttctgat acatcagtgg
    38221 ctgggttgcg ctcggctcgt cggcttcgtt gtggccgtga cggcggtagc ataccaggtc
    38281 gatgaagaca tcacgtttaa aggtgttacg gaaatcgagc gccagacggg tcacaaaggc
    38341 aacggcttcc ggatcatccg cgttaacgtg gaaaatcggt gcctgaacca tcttaccgat
    38401 atcagtacag tacggtgtag aacgggcatc cagcgggtta gaggtggtga aaccaacctg
    38461 gttgttgata acgatacgta ccgtaccgcc aacttcataa ccacgcgctt tcgacatgtt
    38521 cagagtttcc tgaaccacgc cctgcccggt cactgcggcg tcaccgtgga tggtgattgg
    38581 cagcactttg ttgctgttcg gctcatcaag tctgtccaga cgggcacgaa cagagccgat
    38641 aacgaccggg cttacaatct caaggtgaga cgggttaaac gccagcgcca ggtgcaccag
    38701 gccgccatcg gtctggaagt cagacgagaa gcccatgtgg tatttcacgt cgcctgtgcc
    38761 gaggtgttct ttatgtttac cggcgaactc gtcgaacaag tcttgcggtt ttttacccag
    38821 cacgttcacc agcacgttca gacgaccacg gtgcgccatc ccgagaacca cttcgcgggt
    38881 gccgctgttg ccagcgtggc ggatcatctc tttaagcatc gggattaacg cgtcaccgcc
    38941 ttccagcgag aagcgttttg cgccagggaa ttttgcgccg aggtaacgct caaggccttc
    39001 agcggcggtc agttcgctta agaagcgttt tttctcttcg ctattgaaag tcgcgcgacc
    39061 agactcgata cgctgttgga tccagcgttt ttcttcggtg ctggtgatgt gcatatactc
    39121 ggcaccaatc gggccgcagt aggtttgctt gagggcggaa ataagctcgc cgagtttcat
    39181 ggtttctttg ccgctggcaa atgaaccgac gttgaaggtt tcctggaagt ctgcttcggt
    39241 cagatcgtgg aaagacggat ccagatcggc cactttatct tgctgccaca gtcccagcgg
    39301 atctagattc gcatgctggt gaccacggaa gcggtatgcg ttaatgagct gcaggacttt
    39361 aacctgcttc acattggtgt cagggtcgga gatcgttgaa gagtaacgtg aagcgtcttt
    39421 cgccaggcgg cggaaatatt cacgcgtttg agagtggaat tgatccggtt tgactcccgt
    39481 accaggtaac tgctggaacg tcgaacgcca gttagcgtca accgagtcag gatcggttaa
    39541 gaagtcttca tagagctgtt ctatccagct ctggtttgcg ccagagaggt aagaagagtc
    39601 caaccaggct ttcaaagcgc tgttctgcat cgtgatccct taagcatctt ttttatgctt
    39661 acttcgccgt ggatactacc acgcacagtg atgtgcgtgc cggggttcac ttgcgagctc
    39721 ttgtctacgt agtggctcgc gaaggaacct ttaaaaactg tcttagtgtc aggggcagtt
    39781 tttaaagatt tcctgcattt ctttgttacg cctgatgcgc tgcgcttatc aggcctgcgg
    39841 gtaatgactt gtaggccgga taaggcgttc acgccgcatc cggcactggt tgcctgatgc
    39901 aacgcttgcg cgtcttatca ggcctacagt ttacgcatta cgttgcaaca acatcgactt
    39961 gatatggccg atggcgcgcg tcgggttcag ccccttcgga catacactga cgcagttcat
    40021 gatgctgtga cagcggaata cgctgaatgc atcgctcaaa ccgtcgaggc ggctgtcagt
    40081 ctcggtatca cggctatcga tcaggaaacg atatgccgct aacaagcctg ccgggccgat
    40141 aaacttatcg ggattccacc agaaagacgg acaagaggtt gaacaacatg cgcagagaat
    40201 acattcatac agcccgtcga gtttttcgcg ctgctctggc atctgtaaat gctcgcgagc
    40261 tggcggattt tgtccattat tcaacaggta aggcttaatt ttctcatatt gcgcatagaa
    40321 ttgtcccatg tctaccacca aatcgcggat caccggtaaa cctggcagcg ggcgaatcac
    40381 aatcttcttg cccggctggt tgagtgccga aatcggggta atacaggcca gaccattctt
    40441 gccgttcatg ttcagaccgt cggaaccgca cacaccttca cggcaggagc ggcggaacga
    40501 caggctggga tctttctctt tcagctggat aagcgcatcc agcagcatca tgtcgcgacc
    40561 ttcttccgct tccagggtgt aatcctgcat acgcggagca tcatcaacat ccgggttata
    40621 gcgataaatt gaaaactcga gtctcatttt cctgtctccg cattagtaag tacgaatctt
    40681 cggcgggaat gccgggcgca gtttcggttc catgttgacg cttcggcgcg tcatggattc
    40741 cgactcaggc agatacaggg agtggcacag ccagttttca tcatcacgat ccgggaagtc
    40801 gaagcggcta tgtgcgccac ggctttcggt acggaagttg gcagaaacag ccgttgcata
    40861 cgccgtttcc atcaggttat ccagttccag gcactcaacg cgctgggtgt tgaactcgct
    40921 ggaagtgtca tccagacggg catttttcag acgctcgcgg atcactttca actgctcaag
    40981 ccctttcgcc atcgcatcac cttcacggaa gaccgagaag ttatgctgca tacattcttg
    41041 cagcgcttta cggatcgcca ccggatcttc accgttacga ttattgttcc accggttcag
    41101 gcgatccaga gacgcttcaa catcagactc gctggcatcg tgcagtgcgc cctgctcggc
    41161 gatagactct tgcagatgca gacctgccgc gcgaccaaag accaccaggt ccagcagcga
    41221 gttgccgccc agacggttag cgccgtgtac cgatacacaa gcgatttcac caacggcaaa
    41281 cagtcccgga acaaccacat cttcgccttt ctcattcaca gtcagcgcct gaccggtaac
    41341 tttggtcgga ataccgccca tcatgtagtg acaggttggg ataaccggaa tcggctcttt
    41401 caccggatcg acgtgagcga aggtacggga aagctccagg atacccggca gacgggattc
    41461 gagaacttct ttacccaggt gatcgagttt cagtttcgcg tgtggccccc acggaccatc
    41521 acagccgcga ccttcacgga tttcaatcat gatggaacgc gccaccacat cacggcccgc
    41581 caggtctttg gcgttcggcg cataacgctc cataaagcgt tcgccatgtt tgttcagcag
    41641 ataaccgcct tcaccacggc aaccttcggt gaccagtacg cccgcaccgg caatgccggt
    41701 cgggtggaat tgccacattt ccatatcctg caccggtacg cctgcgcgga tagccatgcc
    41761 gacaccgtcg ccagtgttaa tgtgggcgtt agtggtggac tgataaatac gccctgctcc
    41821 gccagtcgcc agcacggtag cgcgggcttt gaaataaacc acttcaccgg tttcgatgca
    41881 cagtgcggta caacccacca ccgcgccatc ctggtttttc accagatcca gcgcatacca
    41941 ctcggagaaa atggtggtgt ggtttttcag gttctgctga taaagcgtgt gcaacagtgc
    42001 gtgaccggta cggtcagcag ccgccgcagt gcgtgccgcc tgctcgccgc cgaagttttt
    42061 cgactgaccg ccaaacggac gttgatagat acgaccatca tcgagacgcg agaacggcag
    42121 gcccatgtgt tcgagttcca gaatcgcttc cggcccggtt ttacacatat attcaatcgc
    42181 gtcctggtca ccgatatagt ccgacccttt tacggtgtcg tacatatgcc attcccagtt
    42241 atcttcatgg gtattaccca gcgcaacggt aatgccgcct tgcgcagaaa cggtatggga
    42301 acgggtcggg aagactttag agagcagggc acaggtctgg ccgctctggg aaatttgcag
    42361 cgccgcgcgc atacctgcgc caccggcacc aatcacaact gcatcaaatt ctctgactgg
    42421 caatttcatc acacacccca caccacaacg aatccataaa tcacgtaaac caccagtgca
    42481 acgacaatca ccagttgcag catcaggcgc aaggccagcg gtttaacgta gtcggtcaac
    42541 acctgccaca tgccgatcca ggcatgaatc aagatagaaa acagcgccag cagggtgaac
    42601 actttggtga acgcagaggc gaagaaaccg atccagactt catatgtcag ttcgccactg
    42661 gtggcgaaaa aaccgaccat ataaatgatg tagagcgtca ggacgatagc ggtagcgcga
    42721 acgaggatga aatcatgtac gccattgcgt cctaatgcgg aggcgttgct taccatacga
    42781 ggactcctgc gagaagtgaa agcacgacag taataacaaa ggagattttg gcggagcgtt
    42841 tacccgcttc gaatgtttct tccagatagc caaaatccat catcatgtgg cgaataccta
    42901 cgacgacgtg atacgccaga gcggtaagga tgccccacat gataaatttg acgaagaagc
    42961 tgcccataat cgcggaagct tgctcgaaac cttcagggga agagaggctg gtacccagaa
    43021 gccactgcag gatgcccact gcaacaaagg tgatcacacc ggaaacgcga tggagaatgg
    43081 acgctatcgc cgtgatgggg aaccggatgg tctgtaggtc cagattaaca ggtctttgtt
    43141 ttttcacatt tcttatcatg aataacgccc acatgctgtt cttattattc cctggggact
    43201 acgggcacag aggttaactt tctgttacct ggagacgtcg ggatttcctt cctccggtct
    43261 gcttgcgggt cagacagcgt cctttccata actgcgcgtc atgcaaaaca ctgcttccag
    43321 atgcgaaaac gacacgttac aacgctgggt ggctcgggat tgcagggtgt tccggagacc
    43381 tggcggcagt ataggctgtt cacaaaatca ttacaattaa cctacatata gtttgtcggg
    43441 ttttatcctg aacagtgatc caggtcacga taacaacatt tatttaattt ttaatcatct
    43501 aatttgacaa tcattcaaca aagttgttac aaacattacc aggaaaagca tataatgcgt
    43561 aaaagttatg aagtcggtat ttcacctaag attaacttat gtaacagtgt ggaagtattg
    43621 accaattcat tcgggacagt tattagtggt agacaagttt aataattcgg attgctaagt
    43681 acttgattcg ccatttattc gtcatcaatg gattctttac ctgcaagcgc ccagagctct
    43741 gtacccaggt tttcccctct ttcacagagc ggcgagccaa ataacaaacg ggtaaagcca
    43801 ggttgatgtg cgaaggcaaa tttaagttcc ggcagtctta cgcaataagg cgctaaggag
    43861 accttaaatg gctgatacaa aagcaaaact caacctcaac ggggacacag ctgttgaact
    43921 ggatgtgctg aaaggcacgc tgggtcaaga tgttattgat atccgtactc tcggttcaaa
    43981 aggtgtgttc acctttgacc caggcttcac ttcaaccgca tcctgcgaat ctaaaattac
    44041 ttttattgat ggtgatgaag gtattttgct gcaccgcggt ttcccgatcg atcagctggc
    44101 gaccgattct aactacctag aagtttgtta catcctgctg aatggtgaaa aaccgactca
    44161 ggaacagtat gacgaattta aaactacggt gacccgtcat accatgatcc acgagcagat
    44221 tacccgtctg ttccacgctt tccgtcgcga ctcacatcca atggcagtca tgtgtggtat
    44281 taccggcgcg ctggcggcgt tctatcacga ctcgctggat gttaacaatc ctcgtcatcg
    44341 tgaaattgcc gcgttccgcc tgctgtcgaa aatgccgacc atggccgcga tgtgttacaa
    44401 gtattccatt ggtcagccat ttgtttatcc gcgcaacgat ctctcctatg ccggtaactt
    44461 cctgaatatg atgttctcca cgccgtgcga accgtatgaa gttaatccga ttctggaacg
    44521 tgctatggac cgtattctga tcctgcacgc tgaccatgaa cagaacgcct ctacctccac
    44581 cgtgcgtacc gctggctctt cgggggcgaa cccgtttgcc tgtatcgcag caggtattgc
    44641 ttcactgtgg ggacctgcgc acggtggtgc taacgaagcg gcgctgaaaa tgctggaaga
    44701 aattagctcc gttaaacaca ttccggaatt tgttcgtcgt gcgaaagata aaaatgattc
    44761 tttccgcctg atgggcttcg gtcaccgcgt gtacaaaaat tacgacccgc gcgccaccgt
    44821 aatgcgtgaa acctgccatg aagttctgaa agagctgggc accaaagatg acctgctgga
    44881 agtggctatg gagctggaaa acatcgcgct gaacgacccg tactttatcg agaagaaact
    44941 gtacccgaac gtcgatttct actctggtat catcctgaaa gcgatgggta ttccgtcttc
    45001 catgttcacc gtcattttcg caatggcacg taccgttggc tggatcgccc actggagcga
    45061 aatgcacagt tacggtatga agattgcccg tccgcgtcag ctgtatacag gatatgaaaa
    45121 acgcgacttt aaaagcgata tcaagcgtta atggttgatt gctaagttgt aaatatttta
    45181 acccgccgtt caaatggcgg gttaattttt atatgcctaa acacaaaaaa ttgtaaaaat
    45241 aaaatccact atcggacatt catatattct aaaaaaaaat acatcggtat aaattataaa
    45301 caaatagatg atttttaatc atctatcttc ctctcagtta catgaataaa ccaccaaata
    45361 tagcgcaccc ctataattcc caatacttta ttcatcaaac atttaattca taccatcaat
    45421 taattacttt tacatttaat aaaattaata atatttttaa gaaaaactaa aaacattcga
    45481 caatcaaata agatattctt gcaccgatat acttttttct tttctgttaa aggactcagt
    45541 tatgtttaaa ggacaaaaaa cattggccgc actggccgta tctctgctgt tcacagcacc
    45601 tgtttatgcg gctgatgaag gatccggtga aattcacttt aaaggtgaag ttattgaagc
    45661 accgtgtgaa atacatcagg atgatattga taaagaggtt gaactcggtc aggtgaccac
    45721 cagccacatt aatcagtcac atcacagcga tgccgttgct gtcgacctgc tcttagtcaa
    45781 ctgtgatctg gaaaactcca gcaacggttc cggtggcaag atttctaaag ttgcagtcac
    45841 ctttgatagc tcagcgaaaa ccaccggcgc agatccgatt ctcaacaaca ccagcacggg
    45901 tgaagccacc ggcgtcggcg tacgtttaat gaataaagat caaagcaata tcgttttagg
    45961 caccgctact ccagatatcg acctggctcc gacctccagc gaacaaacgc tgaatttctt
    46021 tgcctggatg gaacaaattg atcaggctac acctgtaacg ccaggcgcag ttacggcgaa
    46081 tgcaacctac gtgctggatt ataaataagt attactttgg cggcgctatc gccgcctttc
    46141 ataaaatatt tatcaggtat ggacaccgtg aatatttatc gactctcttt tgtatcctgc
    46201 ctggtcgtgg cgatgccttg cgcattggcg gtcgaattca atctgaatgt tctcgataaa
    46261 tcaatgcgcg accgcattga tatttcatta ttaaaggaaa aaggagtcat tgctcccggt
    46321 gagtattttg ttagcgttgc ggtaaataat aaccaaatca gtaacgagca aaagattaac
    46381 tggcacaaaa atgacgataa aaccattccg tgcatcaatg atttactggt cgataaattt
    46441 ggcttaaaac ctgaagtccg tcagtcgtta ccattgataa atcagtgcgt cgattttagc
    46501 tctcgacctg aaatgctctt caatttcgat caagccaatc agcaactgaa tattagtatt
    46561 ccgcaagcct ggctggcgtg gcactcagaa aactggaccc caccctccac atggaaagaa
    46621 ggtgtcgccg gtgtcctgat ggattacaac ttgtttgcca gcagctaccg cccacaggat
    46681 ggcagcagca gcactaatct taacgcctat ggaacagccg gaatcaacac gggggcatgg
    46741 cgcttacgca gtgattatca gttgaatcat actgatagcg atgataacca tgaacagtca
    46801 ggcgaaatat cacgtaccta tctttttcgc ccgttaccgc aattaggttc aaaattaacc
    46861 ctcggcgaaa cggattttag tcccaatatt ttcgacggtt tttcttatac cggcgcggca
    46921 ctggcaagtg acgatcgaat gttgccatgg gaactacgcg gctacgcccc acaaattagc
    46981 ggtattgcac agaccaatgc cacggtgacg atcagtcaat caggccgcgt catttaccag
    47041 aaaaaagtcc caccaggccc atttatcatt gacgacctta atcagtctgt tcagggcaca
    47101 ctggatgtca aagtgacgga agaagatggt cgggtgaaca atttccaggt ttcagcagca
    47161 tcgacgccct tcctgactcg tcagggacag gttcgctata aactggccgc gggtcagcca
    47221 cggccctcca tgtcacatca aactgaaaat gaaacctttt ttagcaatga agtttcctgg
    47281 gggatgctgt caaacacctc gctgtacagc ggcctgctgc tttctggtga tgactaccat
    47341 tctgccgcaa tgggtattgg gcaaaatatg ctgtggcttg gtgcgctgtc gtttgatgtc
    47401 acgtgggcca gtagccattt tgatactcag caggacgagc ggggcttaag ctaccgtttt
    47461 aattacagca aacaagtgga tgccactaac agcacgattt ccctcgccgc ttatcgtttc
    47521 tccgatcgtc attttcacag ctacgccaac tatctggatc acaaatacaa cgacagcgat
    47581 gcgcaggacg aaaaacagac gatcagctta tctgtgggcc aaccgattac cccactaaac
    47641 ctcaatcttt acgccaacct gctacatcaa acctggtgga atgcagacgc ctccacgacc
    47701 gccaacatca cggcaggttt taatgttgat attggtgact ggagagatat ctctatttct
    47761 acgtcattca atacaaccca ttacgaagat aaagatcgcg acaaccagat ttacctgtcg
    47821 atttcgctcc ccttcggtaa cggtggtcgg gttggctatg acatgcaaaa cagtagccac
    47881 agcaccacac accgcatgtc gtggaacgat acgctggatg aacgtaatag ctggggcatg
    47941 tctgccggac tgcaatccga ccgtcctgac aatggagccc aggtgagcgg taactatcag
    48001 cacctgagtt cagcgggtga gtgggatatt tctggtacct atgccgccaa tgattacagt
    48061 tccgtcagca gcagctggag cggttctttc accgcaaccc aatatggtgc agcgtttcat
    48121 cgccgcagct ccaccaatga accacgcctg atggtcagca ccgatggcgt ggcagatatt
    48181 ccggttcagg gcaatctcga ctacaccaac cattttggca ttgcggtggt gccgttgatt
    48241 tccagttacc agccttccac cgtggcggtg aacatgaatg acttacccga cggcgtaaca
    48301 gttgcagaaa acgttatcaa agaaacgtgg attgaaggcg cgataggtta caaatcactg
    48361 gcttcccgtt ccggtaaaga cgttaacgtc atcattcgca acgccagcgg tcagtttcct
    48421 cccctcggag cggatatccg ccaggatgac agcggcatta gcgtggggat ggttggcgag
    48481 gaaggacatg cctggttaag cggagtcgct gaaaatcaaa agtttaccgt ggtctggggt
    48541 gatagccagc attgctcgct ccatcttcct gaacatatgg aagacaccgc aaatcgcctg
    48601 attttacctt gtcattaatt aaggaaatga aaatgacatt tatgaaagga ctaccccttt
    48661 tgttgttggt tgccagtctg tgcagccacg ctgcactaca acccgatcgc actcgtattg
    48721 tgtttaacgc taatgataaa gctaccagtc tgcgggtgga taatcgtagc gataaactcc
    48781 cctacctcgc ttattcctgg ctcgaaaatg aaaaagggga gaaaagcgat gatcttctgg
    48841 tcgccctgcc gccgatacaa cgtctggaac cgaaagcaac aacacaggta cggatagtaa
    48901 agcaagcctc gaccacaaaa ttaccgggcg atcgcgaaac gctatttttc tacaatatgc
    48961 gtgaaattcc tcccgccccg gaaaaaaaca gcgaccatgc cgttctgcaa gtcgccattc
    49021 agagtcgtat caaggtgttc tggcgaccgg ctgcattacg caagaaagca ggagaaaagg
    49081 tcgaactgca gttacaggtc agccagcagg gcaaccaact gacactgaaa aatcctaccg
    49141 cgtattatct gaccattgcc tacctgggac gaaatgaaaa aggcgttctc cccggcttca
    49201 aaaccgtgat ggttgcgcct tttagtacgg tcaacacaaa caccggaaat tatagcggca
    49261 gccagtttta tctcggttat atggatgatt acggcgcgtt acgcatgacg acgctcaact
    49321 gcagcggaca atgccattta caagccgtgg aggcgaagaa atgagtgctg gcaagggagt
    49381 gttgctcgtc atctgtctgt tatttctgtc gttgaagtct gcgcttgccc tgaactgcta
    49441 ctttggttca tccggtggct cggtagaaaa atcagaagcg attcaacctt ttgctgtacc
    49501 aggcaatgcc aaactcggcg ataagatctg ggaatctgac gatattaaaa taagatctgg
    49561 gaatctgacg atattaaaat tcccgtctat tgtgacaaca ataccaacgg caactttgaa
    49621 agcgaacatg tctacgtatg ggtaaacccc tacccggggg tgcaggatcg ttattatcaa
    49681 ctcggagtaa cttacaatgg cgttgattat gacgccaacc agggaaaaag ccgcatcgac
    49741 actaaccagt gtatcgacag taagaacatt gacatttata cccctgatgg gatggcagaa
    49801 caaaatttgc tcgggtgatc ccgctaatat tcacatgtcg cgaacctttc tcgctcgcat
    49861 gcggttatac gtcaaaatac gagaaatgcc gccgcatgat tatcaaagta cgcttagcga
    49921 ctatatcgtc gtgcaatttg acggtgccgg tagcgttaac gaagacccta ctgcccaaaa
    49981 cctgaaatat catattactg gtctggaaaa cattcgcgtg ctggattgca gcgtcaattt
    50041 ttccatttca ccggaaacac agatgattga ttttggtaaa tttaatttgc tggatattcg
    50101 tcgccacaca atgtcgaaaa cattcagtat taagacgaca aaatcacaaa atgaccaatg
    50161 caccgacgga tttaaagtca gttcctcctt ttataccgaa gaaacgttgg ttgaagaaga
    50221 taaagcattg ttgattggta atggtctgaa gctacgtttg ctggatgaaa acgcctcgcc
    50281 ttataccttc aataaatatg ctgaatacgc cgatttcacc agcgacatgt tggtctacga
    50341 aaaaacctat acagctgaac tttcgtccat tgcaggtacc cccatcgagg ctgggccctt
    50401 cgatacagtg gtgcttttta aaattaacta taactgattt gccatgatgc cctttgcagc
    50461 cggaaagggc atctctttca gaaaattgct gattttcccc ttttccttga ttgctgttat
    50521 tttttcctag cccggctttc tttatgctgt atcgatacac caaagagaaa ataatgaggg
    50581 agcgaaggat gccagttttg cagtggggaa tgttatgtgt gctatcactt ctcctttcta
    50641 ttggtttcct cgcggtacat ctcccggcgg cgctattact tgggccgatg atcgccggga
    50701 tcatcttcag tatgcgcgga attactctgc aacttccccg ctccgctttt ctcgccgcgc
    50761 aggccattct tggctgcatg attgcgcaaa acctcactgg ttcgattctc accacgctgg
    50821 ccgccaactg gccgatcgtg cttgctattt tactggtgac gctgctttcc agcgccatcg
    50881 tgggttggtt attggtgcgc tatagctcac tgcctggaaa taccggtgcc tggggttctt
    50941 ctccaggcgg cgcagcggca atggtcgcta tggcgcaaga ttacggcgca gatattcgcc
    51001 tggtggcgtt tatgcaatat ctgcgagtgc tgttcgtcgc gggggccgct gttctggtca
    51061 cccgcatgat gctgggtgat aacgctgaag cggttaacca gcaaatcgtc tggttcccgc
    51121 cagtgagcat taatctcctg cttacgattt tgctcgcagt cgttgccggt acggcgggat
    51181 gcatgctgcg tcttccttcc ggcacgatgc tcatcccgat gctggcgggc gcagtgctcc
    51241 agtctggtca gctcatcacc atcgaactac cggaatggct gctggcgatg gcgtatatgg
    51301 caattggctg gcggattggt cttggtttcg ataaacaaat cttactgcgg gcactacgcc
    51361 cgctaccgca aatcctgctg tcgatttttg ctctgctggc tatttgtgca ggtatggcgt
    51421 ggggactgac ccggtttatg catattgatt ttatgactgc ctacctcgcc accagtcccg
    51481 gcgggcttga taccgtggcg gtcatcgccg cagggagcaa tgccgatatg gcgcttatta
    51541 tggcgatgca aaccctgcgc ctgttcagta ttttgctgac ggggccagcc attgcgcggt
    51601 ttatttcaac ctatgcgccg aagcggtcgg cctagtgctg gcagcctgga caccagtaaa
    51661 acgggcgcga ggacaacgtg gttttctcaa tgatgccgcc acagcgttcg cacagttcgc
    51721 catcacgatg aaaaacctta aagcgaaaca gcgccccatg atgcttattc tcatccacct
    51781 gcccccgcgt agcgtaagaa aaccgaggaa tctccagtaa cgcgtgtgcg agtgcatcca
    51841 gttgcgccgc attgagatct ttcgctttat gatttccagt caacccaacc tgccagagga
    51901 tctccacccg caaataattg ccaagcccag ccagaaacgc ctgatcgagc agtaatcctg
    51961 caaactgacg gttacgaaag cgcggcgaca ataaccgttc ctttaccacc tccggcgtca
    52021 gattcggatc cagcacatcg ggaccaacgc gttgtaaaaa cggatgcgtg gtcagttgtt
    52081 ccggcctcaa catctcaata tccgaggcgc tataaagcag aatggttttg tcagccgttt
    52141 gcagttttac ccgcaatact cgcgtggtct gcggctcttc gccggtatca accacgcgcc
    52201 agacgccgta aagctgatta tggctgtaga gcgttaaacc gttgggaaaa tgagttaaca
    52261 acgccttacc acgcgtttcc acatgggtaa cgtgttgacc gataagttgt gattgataag
    52321 tttttaactg cgggaaggca aaccagacat cagttagtgg tttgcctttg atcgccgcct
    52381 ccaggttatc cgctgcacgg cggatctccg ggccttcagg catggtgata tccttttttc
    52441 attctttatt aagcggggtt atgctgcgac aacaatcccc tttttggcaa atgtagctcg
    52501 taatcggcgg gcgaacgcca gagcgtgctc gccgtcgcca tgcaggcaga ccgtttgcgc
    52561 cgcgaccgtt gcccattcgc cggtgatact tttgactctg ccgtgttgca ccatctccag
    52621 cgtttgcgcc aacgcctgtt cttcgttttc aatcaacgcg cctggctgac ttcgcggcac
    52681 cagcgagccg tcagcctgat aaccgcgatc ggcaaacact tcctcgcgcg ttgtcaggcc
    52741 atattgcttg cctgcacgaa tcagctcgct tcctgccagc ccaacgagaa ccaatgccgg
    52801 atcgcaagcg tataccgctc tggcgatggc gtctgccagt tgtgcttctt tcgccgcctg
    52861 gttgtacaac atgccgtgcg gtttgacatg acgcattacg ccgccttgcg cacgggcaat
    52921 cgttgccagc gcgccaattt gatacagcgt ctgggcgtaa acggtttccg gcggcagctg
    52981 catggcgctg cgaccaaaat tttccctgtc gggaaaactc gggtgagcgc caatcgcgac
    53041 accatttttt attgcttcac gcacgcaagc ctgcatggtt tgcgcatcgc ctgcatgaaa
    53101 tccacaagca atattggcag aggaaaccag cgttaatagc tctgcgtcgc tggcgcagcc
    53161 ttcgcccaga tcggcgttca ggtcaatttt cattgtgcag ccgccacgct aattgttcaa
    53221 aataacgttg ctgatcttgc cgcgctttca gtgcctcttc cagtgaacac tggacaaaat
    53281 gaatcggctg accgagcgga atttgcgcca gatggtacat atcagcctca atgatacagg
    53341 caatacgtgg ataaccaccg gtggtctgtg cgtcgttcat caacacaatc ggctgcccgt
    53401 tatgcggcac ctgcaccacg cccggtaaca aaccgtgaga taacagttcg cgatcggtga
    53461 tgcgttttaa aatttgcccc tgtaaacgat agcccattcg gttactttgc gagctaagct
    53521 gccagggcga acgccagaat gcatcctgcg aggcgcgatc gaactcatga tattccggcc
    53581 ccggcaaggc gcgaatgcgg ttgccccaca gcagctgttt aacgccctgc gcttccataa
    53641 aatcacgctt cgatttgcca atcgggagtc agtcaccatc cttcagtaaa cggccttcca
    53701 gcccgccaat ccccactttg agatcggtgc tgcatgaccc cattaccggc ggaacatcaa
    53761 taccacccgc gaccgccaga taactgcgca tcccgtgctg cgggcgttta agcgttaaac
    53821 gctggcctgc tttcatcggc aatcgccagc cggtccagac ggcgttatca tccaaccgcg
    53881 cttcgcaacc tgcacccgtc agggcaaacc acccatcagt ttcgaactca actgttaact
    53941 gaccgagcgt gatctccagc gcgggggcat tggcgtcatt acccaccagc aggttagcaa
    54001 tgcgcagcgc aggcatatcc agtgcgccac agtggctgat acccgactgg caaaaaccgt
    54061 gacgaccgcc atcctgcaca gtggtgtaca tgcccgcacg aataatcttc agcatattcc
    54121 ctccttctgc ggtacaaatc gcacgctgtc tcccggacgt aataagatgg gttcgtcacg
    54181 cgccggatca aacaggctga gtgaggtatg accaatcaac tgccagccac ccggcgttgc
    54241 cagcggataa acaccagtct gcggcccgcc gatcccgaca gaacctgccg gaacgagcaa
    54301 gcgcggttca gcgcgccgtg gcgtgtgtag ttgttccggc aaactcccga gatacgggaa
    54361 gcccggttga aaacctaaaa accagaccac gtattccacg gaggagtgca attcaacaac
    54421 ctgtttttcg ctcaacccgc aatacgccgc gaccaccgcc aaatccggtc cgcctgcacc
    54481 accgtaaacc accggaattt caataaagcg agactcaggt tccagggcct cgctctcctc
    54541 ccaccagcgt tgcaaacgct ctatggcatc cagcgccagc gactcaggat tacgcaaaat
    54601 caccgtgata ttgttcatgc cgggaatggc ttcaaccaca ttcggcatat ccaccagacg
    54661 ctgcgccagt cgccagatcc gtttctggct agccagcgtc accggcggtt ccagttccag
    54721 cactaccgcc gtttcaccta tcagataaca acgcgctcgt tgcactttcg atccctctga
    54781 ttattcgtta tgcaggatta ggaatatcaa taaaggtcac atcaagatcg gtattttcat
    54841 tcagccactc gctcaatgcg cgaataccac cgcgttcagt ggcatggtga cctgcagcat
    54901 aaaaatgcaa tccctgctcg cgggctgaat gaatggtctg ttcagaaact tcgccagtaa
    54961 taaaagcatc cacgccaaaa cgcgcggcgc tatcgataaa actttgcccg ccgcccgtgc
    55021 accaggcgac gcgctggacc acctcaggtc cggtatcgcc acaccataac ggcttacgtc
    55081 ccagacgcgc ttcaatccag gaggctagtt ccattcccgg cacaggcatg gtcagctctc
    55141 cccacggcac caacggctca atttcgccca tgaccgtgat ccccagtaac gccgccagct
    55201 gtgcgttatt gcccagctca ggatgtgcgt caagcggcaa atgccagcca tacaggttga
    55261 tatcattcgc cagcaacgtt tttaaacggt tacgcttcat tccgcgaatg accggagact
    55321 ctcctttcca gaagtagcca tgatgcacaa tgaccgcatc cgcgcccaga cgcactgcct
    55381 catcgagcaa agcctggctg gcggtgacac cggtaacaat tttttgcacc gtctctttgc
    55441 cttccacctg caaaccattc ggcgcgtaat cactaatcgc cgcgctgttc agtttttcgt
    55501 taatcagttg ttccagttcg gtgtttttca tcttcgcgcc ttccgtattc tgatagctac
    55561 accgttaaca tagcgttaca tcgcctgttc gtcgatagcc aatcttttga catattaaga
    55621 aacaaacccg acgccaaaag acgcaaattg tttatatcat cacgcttcag ctaatgtata
    55681 tttatgcaat cagacagcat attgcataaa taatcagatc tgtatcaaag ttttaacttt
    55741 aaggaaacac aagcacgccg cgcaactatt acgtgacgtt gccgtcaatt gtggaattat
    55801 tatgaataaa cacgcatcac aaccgcgcgc tatttactat gtcgttgcgc tgcaaatctg
    55861 ggaatatttt agcttttacg gcatgcgtgc cctgctgatt ctctatctca ccaatcaact
    55921 aaaatacaac gatactcacg cctacgagtt atttagcgcc tactgttcgc tggtgtatgt
    55981 cacgccaatc ctcggtggct ttttggcgga taaagttctc ggcaatcgca tggcggtgat
    56041 gctgggggcg ttgttgatgg cgatcggtca tgtggtgctg ggtgccagtg agatccatcc
    56101 gtcattcctc tatctgtccc tggcgattat cgtctgcggc tatggcctgt ttaaatctaa
    56161 cgtaagctgt ctgctcggcg agctgtatga gccaaccgat ccgcgtcgtg atggcggttt
    56221 ctcgctgatg tatgcggcgg gtaacgtggg gtctattatc gcacctatcg cctgtggtta
    56281 cgcccaggaa gagtacagct gggcgatggg ctttggcctg gcggcggttg gaatgatcgc
    56341 gggtctggtc attttcttat gtggcaatcg tcatttcact catacccgcg gcgttaacaa
    56401 aaaagtactg cgtgcgacaa actttctcct gccgaactgg ggatggctgc tggttctgct
    56461 ggtggcaacg cctgcactga ttaccgtgct gttctggaaa gagtggtcgg tatacgcctt
    56521 aattgtcgcg actattattg gcctgggtgt actggcaaaa atttatcgca aagcagaaaa
    56581 ccagaaacag cggaaggagc tggggctgat tgtgacgctc accttcttca gtatgttgtt
    56641 ctgggccttc gcacaacagg gcggtagctc gattagcctt tatatcgacc gcttcgttaa
    56701 ccgcgatatg tttggttata ccgttccgac cgcgatgttc cagtcgatta atgccttcgc
    56761 agttatgctg tgcggtgtgt tcctggcgtg ggtggttaaa gaaagcgtcg caggtaatcg
    56821 taccgtgcgc atctggggaa aatttgctct tggccttggc ctgatgagcg ccggattctg
    56881 cattctgacc ttaagcgccc gctggtccgc aatgtatggt cactcttctc tgccactgat
    56941 ggtgttaggc ctcgcggtga tgggctttgc ggaactgttt atcgacccgg ttgccatgtc
    57001 gcaaattacg cgcattgaaa ttccgggcgt gaccggcgta ttaaccggca tctacatgct
    57061 gctttctggc gcgatcgcga actatctggc gggcgtgatt gccgatcaga catcgcaggc
    57121 ttcgtttgat gcttccgggg cgatcaacta ctccatcaat gcatatattg aagtatttga
    57181 tcaaattacc tggggcgcac tggcgtgtgt aggactggta ctgatgattt ggctgtatca
    57241 ggcgctgaaa ttcagaaacc gcgcgctggc gctggagtca taataggcca gcaccgatgc
    57301 ctgatgcgac gcttgacgcg tcttatcagg cctacgtatt tcctgcaatt tattgaattt
    57361 gcacaaattt gtaggctgga ttaggcgttc acgccgcatc cggcatctgg gctcgattgc
    57421 ctgacgcgtc tgttatttcc ccttccgcgc cgcctcatac gctgccaacg tttgtactct
    57481 cgcctctttg tgctcgacta tcggttgcgg ataatccagc ttcacacctg ctttctgcgc
    57541 ccacttccac ggctcatgca ccgctttccc tggtacatcg cgcagctccg gtaaccatcg
    57601 acgaataaac tcgccctcac ggtcaaattt ctcgccctga gttatcggat tgaaaatacg
    57661 aaaatacggc gcagcatcag tacccgtaga agccgcccac tgccagccac cgttattagc
    57721 tgccaaatca ccatcaatca gctgcgacat gaaatatcgc tcgccttcgc gccagtcgat
    57781 caacaaatct ttcaccagaa aactggctgt aatcatccgt agccggttat gcatccagcc
    57841 agtgctgtta agctgacgca tagcggcatc aataatcggg tatcccgttt tgcctttctg
    57901 ccaggcctgt aaatgtgcgg gattgctttg ccactgcaca cgatccgtcc aggcaataaa
    57961 tggacgatgt ttacacaacg aggggtaata cgtcatcaga tgacggtaga actcgcgcca
    58021 gatcagctca ctaagccaga cactaccggc cccaccgtcc agcgcctgcg gctgttccgc
    58081 caacaagcga tgcaaacact ggcgaggcga taacccgccc gttgccaagc tggcggacaa
    58141 acggctggtg ccttccactg ccggaaaatc tcgttgttgc tcatactctc cagcaccgtt
    58201 ctcgcaaaac tggcgtaatt gcgcaatcgc cgctttttct tccaccggga aatgcgcagt
    58261 atcgaaagac tgacgaggat aattcagcgt aatggatggc gcgggcttta tcgatccgct
    58321 actacgaact tttggtgcag cgacgcactc cggcatccct tcccgcagcc gtttcagcca
    58381 ggcattctta aaaggcgtaa agactttgta catctcgtga ttgccggtca tcaccgcccc
    58441 cggcggcagg atcacgctgt catcaaatcc ttcacacacc acgttacgca gcgctctttc
    58501 aacgtccaca tcccgcgctc gctcattcac ctcatactga tagttataaa aaagatgagt
    58561 aacgctgttt tccgcgcaca cctgtttaac tatttcgaca ctggcgacaa agtcatccac
    58621 ttcacggaac aataaaggaa tacctttttc cgccagcgcg atttgtagcc cattcagttg
    58681 agcattgata agttcagcct gacgcggcga catgttatgc gtctcccact tgcgtggtgt
    58741 agcgatatac agcgccagca cgcgtgcaga cgaattgcgg caggcggcag ccagtgcgag
    58801 attatcgtgc agacgtaaat cctggcgaaa ccaggccaga tgggtagtca taaaactcct
    58861 gaatggcgca agttaatcac acgcggccaa agttcgaaat aacgctgccc tgaaagccag
    58921 gcgtcaggat aaagcgccat atagtgtttg atacgcatca gcggcgcaag aagtggttgc
    58981 tcgccacggc gataactgtc gataagcgca gccagctgct ggcgttctgt ggaatcaata
    59041 tgcgggcgaa aataaccctg aacgtgcatc aaaacgttgg tgtgatcgcg cgggttggcg
    59101 ggatgagaaa gcaaaacaat tacgcgttgg cggtactggt tgtaaaaatc atccagattt
    59161 tgccactgat gaatatcagc cacaaacggg ccaagtttgc ggtactcggg ctgagaatgg
    59221 gccagaaaga cgagtttata gcggctgtga taatcgagaa gcgccccgcg cgttaagcca
    59281 tgttctttca gtcgatgtaa ctcatgaagg gcgaagatac ggattagggt gctgtcatca
    59341 aatcgttgta gattcattac tgctcctgaa cggtatacag gagtaagtgt aggcgttggt
    59401 aaatgatgtt tgtgaagtta atccgctagc agggcaagat tacgaaagcg cgctccccgc
    59461 aagcaactaa tcaaggtagt taatcatggc tgaacacgat attacgccag aagtcactat
    59521 ttccaaaaca cagcgccgct ataaagtcgg ctatgtcaga gcccgccacc aggaccgcaa
    59581 aaccggtata gcccggtatt attcacaaca tcccagtctg caccttaagg gcgactggct
    59641 gaaagaggcc ggatttgata ccggacgggg tgtcacggtg aagatttcgg aggggggcct
    59701 gaccatcatc gctgacagtg acgaggtgca ggaactgcgg gatgggatgt ttagtgtgct
    59761 gaacgaaagt taaatattaa aaaagctggg aagatttaaa accttcctag ctttttattc
    59821 tgtcttatta taatggatac tcatcatctt ccgatgaatt tataaattgc tccaagttta
    59881 catgaagagt acttaaccta gtttttaact cattaagtga agcgataatt tcatattttc
    59941 caacataatc atatgatgga tcgtattcat tatctatgtc agaagctaaa aatgatgata
    60001 ttcgtagttt aaatttagct tcatctttat tacattcatt ggcataaggt gaatgcccta
    60061 tcatagcata ttcatctaat ctaaaaggat aagatggttt taattcatcg atcaatgaaa
    60121 tttcaatcca tgttctatat ccaggcatta atggactaat ttctttgagc aaaacctttc
    60181 cacttaataa tgtattgaga ttgttcatta ttatgtctcc cacgggaaaa atatatgtct
    60241 aacacttcca ccatgttgat gaacttgtaa atgtggtaaa tcaccatcag gatttcctgg
    60301 tccatgacct gacactctat atgaaggttt tgttacagaa ggatctttta tatcatccca
    60361 atgatatgaa cctgcttttg ttttattttc agtaagatat tccattaagt tcttagtgct
    60421 ggggcctgac tcacctgtca tatttttata attatgacct aaatatctat ccataaacaa
    60481 ttgttcagcg tcacttctag acctaacagt aactttggtt tcactactat ctaataaacg
    60541 attcgctttt tggttactaa cagcacatgg agttaatcca agtggatcag catattttag
    60601 aggattaggt gcataactgt agagatttaa accgcctctc aaccctatcg ggtcagttac
    60661 catgaaccgc ccgacatccg gatcatagta cctgtgcaga ttgtagtgca gccccgtttc
    60721 cctgtcgagg tactgtccct ggtagcgcag gttctgctcc accccgccgt ggtccttctc
    60781 ctgtatcgtg ttaccccaga cctgataccc cgtctcccac acgattttcc cgtcgctgtc
    60841 cgtcatctct tccggcgcgc cgttcacatc cgtgtgaaaa tacagtatcc ggttcgggcc
    60901 ctctttgccc gccttatcca cccgcgccag cggttcataa ctcccctggt ccctgtagat
    60961 gtacaggctg cctgttccct ccggcctgct ctcctgcgcc agccgcattc cgtcccataa
    61021 aaattttgtc cggttgtacg gcttaccgtc agggctgatg tgctgttttt ccgtccgcct
    61081 ccccagtgca tcgtacacat accggtatgt cccccgcgct gtcgtcactt ccaccatccg
    61141 gtgttccgca tcataacggt aactctgcgt tccgctccgg ccctcctttt ccaccgcacg
    61201 gccaaattcg tcataacggt agtgcacacc acggtaagac atcagccggt tgaacggcac
    61261 cacgctgttt tctgccgtgg ccctttcccc cgcgcgcctc cccagcaggt ttcccgccgc
    61321 gtccacctcc gtttccggct ggtgccacac cgggtgccac tgcgcctgct ccacccgccc
    61381 cagcgggtcg tgcgtctccg tgatatgccc ctgcaggtca tacacatagc gccatttgcc
    61441 gccctgaggg tcggtcatcc ccagcaacag tcgttcttcg tcgctccagc ggaatgtcgt
    61501 cacctgtccg gcttcgtcct ggtacgccgt cacctgattc tgcgcatccc actggtgata
    61561 acgtgtgcgc cccagtccgt ccgtcacgca cagcctgcgc tgcgccaggt cccacgccag
    61621 ccggtactgt tcaccgctgt ccgtgtggtg ccccgtcacc cgccatgccg cgtcatccgg
    61681 accttcatac cagccccact ggtaataata cccttcgccg cccggctggc ggtgcagcgt
    61741 catgcagtgg ttttcgtcgt aagcaaactc acgcagcagg tgccccgtgc tgtcgtacac
    61801 gccgttcagg tggccggcct cgtcatagct gtaacgacgc agcagtaaag gtgcagtctc
    61861 agggaaatgc tgataaatct gtgtcacccg ccggggatgc gccgccagtt cgtaatacag
    61921 acggatgcac ggacgctgct gttcgccgct gatttccaca atgcggcccc ggtcgtcgta
    61981 gtacagattc agacagttgc tgttacggtc gcccagcatt ttcagccgct ggcgctgcgg
    62041 gtggtgcggg tcagcctcaa acagaaaaca ctgcccgtca tcgctgctga taagccagtg
    62101 cccctgctca ccacgcctta ccgccagccc ttcaccgggg ctgtaaaacc cgccccccgg
    62161 cagtacggct tccatatcca agcgtcgccc catgcccgac acaaaggtca tgcagttttc
    62221 gtctgcgcta cccggtgtgc acacgaggct gacttcatac attacactcc agccctgacc
    62281 aaacattcct tccgtacgtt cgtcacgact gctgtacacg cgctgccagt ccagcggaaa
    62341 gtggcccggc agactgaaat cgaagtcatc gtcatcggcc aggtatttgg ccccggtggc
    62401 ggcattgacc ggatgcggaa gggcactggt ggccttctgc gtcagcatgc tcatcccgac
    62461 actggcagca aaacaggcga ttttagaaag cattttgccc gggcgaagaa acatcagcgc
    62521 cacggttgcc accagggtta tctgcgacct gccgcttctg atatccctga ccaccagtaa
    62581 cggaccgcca atgcgcacat ccggtgacac aaacgccccg ttccccggct cattcaccac
    62641 cctggcctcg caggtacagc gtgccccgct gcgtgtcgcc ggctggctgt tgatacgcac
    62701 atcccgcgcg ccttccgcga tatattccgc tgcgggcggc tcgtcatcgc ctttgaacag
    62761 attactgatg ctgccaataa ccgcattcac tccctgaaag gcggtttcca gcataccgcc
    62821 cggattcagc aggttaaggg ccgttcccag ggggtcatcc agaaaggcgg cttttttttg
    62881 tgcgagaaac tgcgtcagtg aggaaggatg tttatggcag tcgattttat cgtcaggggt
    62941 cggggtggat cccggggccg cactggccac agtgggctgc catagttccg acacaaaatg
    63001 tgtcacgcca tccaccattt cagaacgccc ttcttccgta ctgagctggt cgtgcagagc
    63061 ccgggacatc agcacaggcc ctgtcaccgg gttaaccgcc agggctatgg cccccgccat
    63121 ttccagatat tccccggcca catcccggaa cgttctggca tcctcctgac gctgcgcttc
    63181 ttcctgttct gccttcgcct gcgcttcctg cgcctctatg tcctgccgcg atgccgcagt
    63241 cgccgctgcc cgcgcggcag gtttgctgtt gatccatgtg tcaccagagc cggtgaggat
    63301 gtaaccttca atctgaggcg ggaaaagcat atttgcgagg gctgaacagg cttcgctgac
    63361 cttttctgtc acgccggtgt acaccatcgc cacacccgcc acaaaaccgg tcagcacgac
    63421 ggccgtgcac cccatcgtga tccccactga cgtcgccagt aacgaggttg tgccgacaat
    63481 gagggcatca ataacgacat ttgcggccac ctccagcacc gcccccagca catctgccat
    63541 aaacgacgta tgcagcaggg catcaccctg tcttgccgcc cagaatgcct cttccatgtc
    63601 ccgttccctg tatgattaat tcgtcctgac ttcctgccgc atcctgaagc gcagattctt
    63661 tttaatttca gcccagcgcg tcatatctgc cggggtcagc ggtgtggttc tggcgtaggt
    63721 gaaggccatc atgacccgct cacccggcaa tacaaatgcc acctgacact ggtacacctg
    63781 ctcgttaccc cggagaaact ggctctgtat ttccaaaccg cctcgttgag ataatattcc
    63841 gtcataatgt aaatagtccg ttttaataaa ggaattattt attgacaggt tctgtttttt
    63901 taatcatgaa tgtaataccc atcaccgctt tacagcacac tttatctttc cagtcataat
    63961 cactaataat attaagcaat tagcaatatt tacaacgacc agagcggata catagttatg
    64021 tatttaacaa tcccaatata ttcactatgc aaataaaacc actagtaaca gacacgctac
    64081 cgcctccata taaaaatgga caccttataa aattgtatct tgcatagaaa aagctgaaat
    64141 atcggatgcg ttttaattat caactataaa tctataagct ttttattaac aaca
//
LOCUS       DAJTSA010000012        60414 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000012 DAJTSA010000000
VERSION     DAJTSA010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.012"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..316
                     /locus_tag="OO369_000806"
     CDS             <1..316
                     /locus_tag="OO369_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5981181.1"
                     /translation="GTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSN
                     SVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVT
                     YP"
     gene            429..1223
                     /gene="panB"
                     /locus_tag="OO369_000807"
     CDS             429..1223
                     /gene="panB"
                     /locus_tag="OO369_000807"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR5981182.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1235..2086
                     /gene="panC"
                     /locus_tag="OO369_000808"
     CDS             1235..2086
                     /gene="panC"
                     /locus_tag="OO369_000808"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR5981183.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2160..3062)
                     /gene="rpnC"
                     /locus_tag="OO369_000809"
     CDS             complement(2160..3062)
                     /gene="rpnC"
                     /locus_tag="OO369_000809"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR5981184.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3336..3716
                     /gene="panD"
                     /locus_tag="OO369_000810"
     CDS             3336..3716
                     /gene="panD"
                     /locus_tag="OO369_000810"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR5981185.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3720..4949)
                     /locus_tag="OO369_000811"
     CDS             complement(3720..4949)
                     /locus_tag="OO369_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5981186.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5013..5453)
                     /locus_tag="OO369_000812"
     CDS             complement(5013..5453)
                     /locus_tag="OO369_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5981187.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5558..6328)
                     /locus_tag="OO369_000813"
     CDS             complement(5558..6328)
                     /locus_tag="OO369_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5981188.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6325..7251)
                     /locus_tag="OO369_000814"
     CDS             complement(6325..7251)
                     /locus_tag="OO369_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5981189.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7360..8022
                     /gene="can"
                     /locus_tag="OO369_000815"
     CDS             7360..8022
                     /gene="can"
                     /locus_tag="OO369_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR5981190.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8063..8599)
                     /gene="hpt"
                     /locus_tag="OO369_000816"
     CDS             complement(8063..8599)
                     /gene="hpt"
                     /locus_tag="OO369_000816"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR5981191.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8805..11195
                     /gene="gcd"
                     /locus_tag="OO369_000817"
     CDS             8805..11195
                     /gene="gcd"
                     /locus_tag="OO369_000817"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR5981192.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11242..12792)
                     /gene="cueO"
                     /locus_tag="OO369_000818"
     CDS             complement(11242..12792)
                     /gene="cueO"
                     /locus_tag="OO369_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR5981193.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGTQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12958..13305
                     /locus_tag="OO369_000819"
     CDS             12958..13305
                     /locus_tag="OO369_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR5981194.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13411..14277
                     /gene="speE"
                     /locus_tag="OO369_000820"
     CDS             13411..14277
                     /gene="speE"
                     /locus_tag="OO369_000820"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR5981195.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14293..15087
                     /gene="speD"
                     /locus_tag="OO369_000821"
     CDS             14293..15087
                     /gene="speD"
                     /locus_tag="OO369_000821"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR5981196.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15125..15487)
                     /locus_tag="OO369_000822"
     CDS             complement(15125..15487)
                     /locus_tag="OO369_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR5981197.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15662..18259)
                     /gene="acnB"
                     /locus_tag="OO369_000823"
     CDS             complement(15662..18259)
                     /gene="acnB"
                     /locus_tag="OO369_000823"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR5981198.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18614..20467
                     /locus_tag="OO369_000824"
                     /pseudo
     CDS             18614..20467
                     /locus_tag="OO369_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20538..21962)
                     /gene="lpdA"
                     /locus_tag="OO369_000825"
     CDS             complement(20538..21962)
                     /gene="lpdA"
                     /locus_tag="OO369_000825"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR5981199.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22287..24167)
                     /gene="aceF"
                     /locus_tag="OO369_000826"
     CDS             complement(22287..24167)
                     /gene="aceF"
                     /locus_tag="OO369_000826"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR5981200.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24182..26845)
                     /gene="aceE"
                     /locus_tag="OO369_000827"
     CDS             complement(24182..26845)
                     /gene="aceE"
                     /locus_tag="OO369_000827"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR5981201.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27006..27770)
                     /gene="pdhR"
                     /locus_tag="OO369_000828"
     CDS             complement(27006..27770)
                     /gene="pdhR"
                     /locus_tag="OO369_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR5981202.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28312..29685
                     /gene="aroP"
                     /locus_tag="OO369_000829"
     CDS             28312..29685
                     /gene="aroP"
                     /locus_tag="OO369_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR5981203.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29728..30582)
                     /gene="ampE"
                     /locus_tag="OO369_000830"
     CDS             complement(29728..30582)
                     /gene="ampE"
                     /locus_tag="OO369_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR5981204.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30579..31130)
                     /gene="ampD"
                     /locus_tag="OO369_000831"
     CDS             complement(30579..31130)
                     /gene="ampD"
                     /locus_tag="OO369_000831"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR5981205.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31218..32111
                     /gene="nadC"
                     /locus_tag="OO369_000832"
     CDS             31218..32111
                     /gene="nadC"
                     /locus_tag="OO369_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR5981206.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32314..32754
                     /gene="ppdD"
                     /locus_tag="OO369_000833"
     CDS             32314..32754
                     /gene="ppdD"
                     /locus_tag="OO369_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR5981207.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32764..33911
                     /gene="tadA"
                     /locus_tag="OO369_000834"
                     /pseudo
     CDS             32764..33911
                     /gene="tadA"
                     /locus_tag="OO369_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33901..35103
                     /gene="hofC"
                     /locus_tag="OO369_000835"
     CDS             33901..35103
                     /gene="hofC"
                     /locus_tag="OO369_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR5981208.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35138..36181)
                     /gene="guaC"
                     /locus_tag="OO369_000836"
     CDS             complement(35138..36181)
                     /gene="guaC"
                     /locus_tag="OO369_000836"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR5981209.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36343..36381)
                     /locus_tag="OO369_000837"
     CDS             complement(36343..36381)
                     /locus_tag="OO369_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981210.1"
                     /translation="MIIKTLKMSARK"
     gene            36406..37026
                     /gene="coaE"
                     /locus_tag="OO369_000838"
     CDS             36406..37026
                     /gene="coaE"
                     /locus_tag="OO369_000838"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR5981211.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37026..37769
                     /gene="zapD"
                     /locus_tag="OO369_000839"
     CDS             37026..37769
                     /gene="zapD"
                     /locus_tag="OO369_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR5981212.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37779..37976
                     /gene="yacG"
                     /locus_tag="OO369_000840"
     CDS             37779..37976
                     /gene="yacG"
                     /locus_tag="OO369_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR5981213.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38076..38465)
                     /gene="mutT"
                     /locus_tag="OO369_000841"
     CDS             complement(38076..38465)
                     /gene="mutT"
                     /locus_tag="OO369_000841"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR5981214.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38525..41230)
                     /gene="secA"
                     /locus_tag="OO369_000842"
     CDS             complement(38525..41230)
                     /gene="secA"
                     /locus_tag="OO369_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR5981215.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41292..41804)
                     /gene="secM"
                     /locus_tag="OO369_000843"
     CDS             complement(41292..41804)
                     /gene="secM"
                     /locus_tag="OO369_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR5981216.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42035..42952)
                     /gene="lpxC"
                     /locus_tag="OO369_000844"
     CDS             complement(42035..42952)
                     /gene="lpxC"
                     /locus_tag="OO369_000844"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR5981217.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43053..44204)
                     /gene="ftsZ"
                     /locus_tag="OO369_000845"
     CDS             complement(43053..44204)
                     /gene="ftsZ"
                     /locus_tag="OO369_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR5981218.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44265..45527)
                     /gene="ftsA"
                     /locus_tag="OO369_000846"
     CDS             complement(44265..45527)
                     /gene="ftsA"
                     /locus_tag="OO369_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR5981219.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45524..46354)
                     /gene="ftsQ"
                     /locus_tag="OO369_000847"
     CDS             complement(45524..46354)
                     /gene="ftsQ"
                     /locus_tag="OO369_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR5981220.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46356..47276)
                     /gene="ddlB"
                     /locus_tag="OO369_000848"
     CDS             complement(46356..47276)
                     /gene="ddlB"
                     /locus_tag="OO369_000848"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5981221.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47269..48744)
                     /gene="murC"
                     /locus_tag="OO369_000849"
     CDS             complement(47269..48744)
                     /gene="murC"
                     /locus_tag="OO369_000849"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR5981222.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48798..49865)
                     /gene="murG"
                     /locus_tag="OO369_000850"
     CDS             complement(48798..49865)
                     /gene="murG"
                     /locus_tag="OO369_000850"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR5981223.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49862..51106)
                     /gene="ftsW"
                     /locus_tag="OO369_000851"
     CDS             complement(49862..51106)
                     /gene="ftsW"
                     /locus_tag="OO369_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR5981224.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51106..52422)
                     /gene="murD"
                     /locus_tag="OO369_000852"
     CDS             complement(51106..52422)
                     /gene="murD"
                     /locus_tag="OO369_000852"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR5981225.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52425..53507)
                     /gene="mraY"
                     /locus_tag="OO369_000853"
     CDS             complement(52425..53507)
                     /gene="mraY"
                     /locus_tag="OO369_000853"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR5981226.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53501..54859)
                     /gene="murF"
                     /locus_tag="OO369_000854"
     CDS             complement(53501..54859)
                     /gene="murF"
                     /locus_tag="OO369_000854"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR5981227.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54856..56343)
                     /gene="murE"
                     /locus_tag="OO369_000855"
     CDS             complement(54856..56343)
                     /gene="murE"
                     /locus_tag="OO369_000855"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR5981228.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56330..58096)
                     /gene="ftsI"
                     /locus_tag="OO369_000856"
     CDS             complement(56330..58096)
                     /gene="ftsI"
                     /locus_tag="OO369_000856"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR5981229.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58112..58477)
                     /gene="ftsL"
                     /locus_tag="OO369_000857"
     CDS             complement(58112..58477)
                     /gene="ftsL"
                     /locus_tag="OO369_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR5981230.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58474..59415)
                     /gene="rsmH"
                     /locus_tag="OO369_000858"
     CDS             complement(58474..59415)
                     /gene="rsmH"
                     /locus_tag="OO369_000858"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR5981231.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59417..59875)
                     /gene="mraZ"
                     /locus_tag="OO369_000859"
     CDS             complement(59417..59875)
                     /gene="mraZ"
                     /locus_tag="OO369_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR5981232.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14470 a        16731 c        14846 g        14367 t
ORIGIN      
        1 tggtactgaa aacgggcaac tccttggtaa tactctcaca ggtaatgatg cagcaaaagg
       61 agtcggtgta ctcatagaag gtttagcaac tagtaaaaat cctctaatga cattgaaacc
      121 taatgattca aattctgttt ataaagatta cgacccgaga ggcaaagacg atacaacagg
      181 aggagtttac ccggatcaag atacaggtat aacataccct ctccatttcc aggccacgct
      241 acaacaggat ggaactatac caatagaagc tggtgagttt aaagccacca gtactttcca
      301 ggtaacctac ccttaataag tccaccgcac cgccatcatc tggcggtgcg gtaattgata
      361 aatcattgcc cccaccaatg tgactgataa aatgccaggc aacacgacat catttatcag
      421 gatacgttat gaaaccgacc accattgctt cacttcagaa atataagcag gataaaaagc
      481 gtttcgcgac catcaccgct tacgactaca gcttcgcgaa actatttgct gatgaagggc
      541 ttaacgtcat gctggtaggc gactcgctgg gcatgaccgt tcagggccac gattccactc
      601 tgccagtaac cgttgctgat atcgcctacc acactgccgc cgtacgtcgc ggcgcaccaa
      661 actgcctgct attggctgac ctgccgttta tggcgtatgc cacgccggaa caagcctttg
      721 aaaacgccgc aacggttatg cgtgccggtg ccaatatggt caaaattgag ggcggtgagt
      781 ggctggtcga aaccgtaaaa atgctgaccg aacgtgccgt tcctgtatgt ggtcacttag
      841 gtttaacccc acagtcagtg aatattttcg gtggctacaa agtacagggg cgcggcaatg
      901 aagcgagcga tcgactactc agcgatgcat tagccttaga agccgcgggg gcacagctgc
      961 tggtgctgga atgcgtgccg gttgagctgg caaaacgtat taccgaagca ctggcgatcc
     1021 cggttattgg tattggtgca ggcaacgtca ctgacgggca aatcctcgtg atgcacgacg
     1081 cctttggtat taccggcggt cacattccta aattcgctaa aaatttcctc gccgaaacgg
     1141 gcgacatccg cgcggctgtg cggcagtata tggctgaagt ggagtccggc gtttatccgg
     1201 gcgaagaaca cagtttccat taaggagtca cgttgtgtta attatcgaaa ccctgccgct
     1261 gctgcgtcag caaattcgcc gcctgcgtat ggaaggcaag cgcgtggcgc tggtgcctac
     1321 tatgggtaat ctgcacaatg gccatatgaa gctggtcgac gaagccaaag cccgcgccga
     1381 tgtggtcgtc gtcagtattt tcgttaaccc gatgcagttc gaccgcccgg aagatctggc
     1441 acgttatcca cggaccttgc aggaggactg cgagaagcta aacaaacgta aagtggattt
     1501 agttttcgcc ccttcggtaa aagagatcta cccgaacggt actgaaaccc acacttacgt
     1561 tgacgttcct ggcctttcga ccatgctgga aggtgccagc cgtccgggac attttcgcgg
     1621 cgtttcgact attgtcagca agctgttcaa cctggtccag ccggacatcg cctgcttcgg
     1681 tgaaaaagat tttcagcaac tggcgttgat ccgcaaaatg gttgccgata tgggcttcga
     1741 tattgagatt gtcggtgtgc caattatgcg cgccaaagac ggtctggcgc taagttcccg
     1801 taacggttat ctgacggcgg aacaacgcaa aattgcgcct ggtctgtaca aagttttaag
     1861 ttcgattgct gacaaattgc aggctgggga acgggatctc gatgaaatta ttaccattgc
     1921 ggggcaagaa ctgaatgaaa aaggcttccg cgccgatgat attcagattc gcgatgccga
     1981 cacattgctg gaagtttctg aaaccagcaa acgggcagta attctggtag ccgcctggct
     2041 tggcgatgct cgcctgatcg acaacaaaat ggtcgagctg gcgtaatact taactggcgc
     2101 tacggctgat ggcgccagtt attaatttac cccacgtcat ctgtcataac acaggtaact
     2161 taaataaggt taattacctt atcaatctca gcaagaggta aatttgccat ctctgcaacg
     2221 tcttcccgag acattccttt acttagaaga cgcagggcga attcttgcct ttcctcctgt
     2281 cttccctgct gaatcccctt ctcaatccct ttctcttcaa accactgcgc cagcgtcatc
     2341 atagacttcc ctcccgtttc cctgtctctc agcacaccat aaaacaaatc cgcttgttca
     2401 gtatgaccgc gttgcagcat atagttttgc atggcaacta actgacttcc gctagtgtac
     2461 ccttcgtcaa tcagcgtgac cagttgctcc agcaataaca ttaagtcgcg ctggcgaata
     2521 tgtttttgca gtagttcgag aatcgcaatc cgccgatgtt gcatgatttc gtcatccggt
     2581 gtgatggtga tatccaccag cgggaaagga ctgttataga cgcgtcgcgc cagctccggc
     2641 gagtaaaaca tatcaaacca gcacattgag agcggataag gtgtggcctc gccctgataa
     2701 aacagtatcg gcaccaccag cggcagctta tcgtggccag cctccagatg ccggtgcatg
     2761 gcagcaatag aataacgcat catgcgaaag gccattttct tatccggctt gctttggtgt
     2821 tcaatcacaa catgcagata accgggattg ccctgcattt gcatggaata gagcacgtcc
     2881 gtgctgtgac ctttcaggca ctcttcaatg aaactccccg actctaaatg aagcgtgttg
     2941 agatcacaaa gttcgcgtaa ttccactggc aaatgtatct ccagaaagtc gcgagccgtc
     3001 tccgcatgca ttaaaaattg tttaaatacc gcgtcatgcg gtgtggtact cggtgcatcc
     3061 atcttccctg tcttccgtgt ggccttgcgt gaaggcagta tccagtcaag gcgtgctatc
     3121 gtcagccacc tctttattgt ttaccgagca gcgttcataa aaatatcaat tcgttacagg
     3181 cgttacatgg cacgcttcgg cgcggtacgc aaactgctga cgcaaatgcc atcagatgag
     3241 tagacactca tcaaaaatcg ggcaatactg cgtgagaatt tcctcaaagc aggcaccgcc
     3301 tgcttcgtta acgacagggt agaaaggtag aagttatgat tcgcacgatg ctgcagggca
     3361 aactccaccg cgtgaaagtg actcatgcgg acctgcacta tgaaggttct tgcgccattg
     3421 accaggattt tcttgacgca gccggtattc tcgaaaacga agccatcgat atctggaatg
     3481 tcaccaacgg caagcgtttc tccacttatg ccatcgcggc agaacgcggt tcgagaatta
     3541 tttctgttaa cggtgcggcg gcccactgcg ccagcgtcgg cgatattgtc atcatcgcca
     3601 gcttcgttac catgccagat gaagaagctc gcacctggcg acccaatgtc gcctattttg
     3661 aaggcgacaa tgaaatgaaa cgtaccgcga aagcgattcc ggtacaggtt gcttgattgt
     3721 tatccctgcg gctggttact caccagccgc gacatcttct ccagcgaatc cgttcttaag
     3781 atataaagtc gttttagtaa caacggatta tcccccggtt ttactttgcc tttcatggtt
     3841 gtcaccgcca ggtgaaatcc ggcatcgttt gctgccttca cggctttgtc attaaagccg
     3901 ccaaacggat acgaaagata ccagacatgt ggattaaatt gcgccagagc gcggcgtgaa
     3961 cgtgcaaaat caaacagaat attgtgctca ctacggctca gtaatatggg tcggcgataa
     4021 ccatctaccc gatgcaaaaa atgggtatgt gactggaaat caaaaacatc gcgaatttcg
     4081 ttaagctcag aaacgctcat aaattgcagc gattttgggt tccacttctg cgggtgacgt
     4141 ttgatgcgtg aggtaacaat aaacaccgtc gccttcatgc catattgttt caacacagga
     4201 tacgcatagc ggctcaccga cttgaggcca tcatcaaagg taatcaccac cgctcgcgca
     4261 gggagattga tcgtattctt cacgtaacct tccagctgag ccatgctcag tgtcgcgtat
     4321 cccctgtcgc gcagccaggc catttggtta ttgaaagcgc gtaccgaggt ggtcgtcgaa
     4381 gtatggcgaa aacgggtgtt ttcttcgtcg cgcagaatat ggtgataagt tagcaccggc
     4441 aggccattat cgggttgggc atccagcgcg ctgatatagg ccagtcgatc gccaatacgg
     4501 atctgatacc aggtttgatt taacctgtct ttcagtttat gcaaaatcgg gtagcgcaaa
     4561 ttgtccgcca gtaccccaaa tggcgcactg cccacactcg gcgcgttgta gaccggcgta
     4621 tctttccagg taattaagtt ctgattactc agcggcttgt tgaggtcacc caaaccgtct
     4681 tcaacttttt gtcgtccctg aactggctcg agatgccctt tatcgataaa ccctttacca
     4741 aagccaaaat taaatgcgta ataacttgcg gcagtgggct ccacggcaat gatttgtccg
     4801 gcccgaatat tccccacggt caccatcttg tcactaattt gcgcccagac cgcagcattt
     4861 tcgatggttt gcatataacg ggcaggtaac gcggcactga cacttgcggt aaacagcatc
     4921 agcaggagaa taacagcttg tttatacatg aaaaaataac cgagggtgaa acaacgcttt
     4981 tattgtagca aaagcgccat cagaatcgcg ggttaatact catacaaatt atgtttgagg
     5041 acgaaaggcg gatttttttg ttgttggtgc caaagactac tcacctccgg cccacccagt
     5101 tcgacaatac actcacgaaa ctctgaactg gtcatggttt cacggcaagc catcatctgt
     5161 tcaattaacg gtaacgtgac accagaaatc acttcgcaac gggagtgttt gtggcttaat
     5221 aatgaagcca cgcgatacgg tggcgctccg gctatatcgg ttaagaagat gacaccctca
     5281 ccagagtccg cttcatgcag agcatcgcac atcatgcggc tgagcatatt agagcttaat
     5341 ccgcgccaga aattcacggc ccggcactga agaagtgccc catgtttttt ctccagcgca
     5401 tccagtatct cttgcgcccg gtcatcgtga caggtaatta cccaacctaa catcgccttc
     5461 ctccttagcc gaaaatcagc ctaccctgac cattgttgat aaagggtgat agccgtcaaa
     5521 actcctctcc cccgaatcca gaggagagga aatagcctta gctacgcaaa ccacgtccac
     5581 gttggatcag cgaccaacag atcaaataaa acgccacaat aaagaccacc agtacgccaa
     5641 aggtagtgac cagcggcaca tcatttatac cgaggaagcc gtagcggaaa ccactgatca
     5701 tataaacgat tgggttcagg tgcgacagcc cttgccagaa cggcggcaac aaagtcagtg
     5761 agtaaaagac cccgcccaaa tacgtgagtg gcgttaacac aaaggttggc accaggctga
     5821 tgtcatcgaa cgttttggca aacacgccgt tcagcaaacc cgcaagggag aatagcaccg
     5881 ccgtgagcac cagggttaag gcaacgaata cccacgaatg cacctgaaac ggcacaaaaa
     5941 acagtgaaat tgccgtcaca agaatgccaa caaacagacc acgcgccacg ccaccgccga
     6001 catatccggc aataatgacg tgagtcggaa ccggcgctac cagcagctct tcaatattac
     6061 gctggaactt ggcaccaaaa aatgatgacg cgacgttagc gtaggcgttg gtgatcaccg
     6121 acatcatgat cagccccggt acgatgaact gcatatagct gaagccatgc atatcgccaa
     6181 tacgcgaacc tatcaggtta ccgaagataa taaagtaaag ggtcatggtg atgactggcg
     6241 gcaccagcgt ctgcacccag atacgcataa agcgatggat ctctttcgcc cagatgcttt
     6301 ttagcgccac ccagtaaaga tgcatcatgc gcgatctcct tgtttttcat taaccagtga
     6361 aacaaacagc tcttccagac ggttagcttt gttacgcata cttaatacct gaatgccctg
     6421 ctcacttaac tgcgtaaata cgctgttgat cccctgctca cgtagcactt caacttccag
     6481 cgtcgcggta tcgaccagtc gatactgata gccatcgagc ttcggtaacg gacttttcgg
     6541 tgcgagatcg agaataaagg tttccgattt cagcttcgcc agcagcgcct tcatcgaggt
     6601 attttccacc agctcaccat gttgaataat gccgatattg cggcacagca tttctgcttc
     6661 ttccaggtag tgtgtggtaa gaatgatggt ggtgcctttg tcgtttaaat ccttcaaaaa
     6721 gccccacatt gagcggcgaa gttcaatatc cacgcctgcg gtcggttcgt cgagaatcag
     6781 tagtttaggt tcatgcatta acgcacgggc aatcattaaa cggcgcttca tcccgccaga
     6841 taacatacgc gcacgttcgt tgcgttttcc ccataaatcg agctgcttaa gatatttttc
     6901 gctgcggatg tacgcctctt tgcgctccac accgtagtac cctgcctgat tcaccacaat
     6961 ttgctgcacg gtttcaaacg ggttgaagtt aaattcctgc ggcaccagtc ccaactgacg
     7021 tttagcgttc acgacatctt tctcgagatc gtaaccaaat acgctgaccc gcccggaggt
     7081 tttatttacc agagagctga taataccgat agtggtcgat ttcccggccc cgttcggccc
     7141 gagaagcgca taaaaatcac ccgcttcgac ctgcaaatct attccacgaa gcgcctgaac
     7201 gccgcctgga tagttttttt taagctgttg aagttccagt gcaatggtca taaattttta
     7261 cttaccttac gttcttacac tttatctgtg gtttaaatcg tcccggagtt gccctatatt
     7321 agccaaacgt aattatttgg ttacaggtcg ttaacctcca tgaaagacat agatacactc
     7381 atcagcaaca atgcactatg gtcaaaaatg ctggtggaag aggatcccgg gttttttgag
     7441 aaactggcac aagcgcaaaa accgcgcttt ctatggattg gatgttccga cagtcgcgtt
     7501 cctgcagaac gtttaaccgg tcttgagccg ggcgaactct ttgttcaccg taatgttgct
     7561 aacctagtca ttcacaccga cctgaactgc ctttccgtgg ttcagtatgc agtggatgta
     7621 ctcgaagttg aacacattat tatctgtggc cactacggtt gcggcggcgt acaagccgcc
     7681 gttgaaaacc cggaactggg gcttatcaac aactggctgc tgcatatccg cgatatctgg
     7741 ttcaaacata gctcattgct cggcgaaatg ccgcaagagc gccgtctgga taccttgtgt
     7801 gaactgaacg tcatggaaca ggtgtataac ctgggccact ccaccattat gcaatcagcg
     7861 tggaaacgcg ggcagaaagt taccattcac ggctgggcct acggcattca cgacggcttg
     7921 ctgcgtgatc tggatgttac cgccaccaac cgcgaaaccc ttgagcaacg ttaccgtcac
     7981 gggatttcca acctcaagct gaaacacgcc aaccacaaat aaaaatgcca tgccggatgc
     8041 aacacatccg gcaacttcac acttactcgt ccagcagaat cactttgcca atatacggca
     8101 gatgacggta acgctgtgcg taatcaatgc cgtaacccac cacaaactca tccgggatcg
     8161 agaaaccgat aaattcaacc gggacgttca cttcacgacg ggacggttta tccagcagcg
     8221 tacaaatcgc caacgacttc ggttcgcgca ggcttaagat ctcacgcact ttcgacagtg
     8281 tattccccga gtcgatgata tcttcaacaa tcagcacgtc cttgccacgg atatcttcat
     8341 ccagatcttt gaggattttc acatcacggg tggtggacat gccgctaccg tagctggagg
     8401 cggtcataaa gtcgacttca tgagatacct gaacttcacg gcacaggtcc gccataaaca
     8461 taaatgagcc acgcagcaga cccaccagca ccatatcgct gccgctgtct ttgtaacgct
     8521 cagtaatctg acgacccagt tcggcgatac gcgctttaat ctccgcttcg gggatcatta
     8581 cttctacagt atgtttcata tctctaacca tatgatttaa aaacaaatca cttcgcacgg
     8641 gcaggttttg gcaccgcaac aaagcgcaag acgcgcagta taccagtaaa actattcatt
     8701 cgcggaatct gttaataaag ctcgaattgt gatgacggtc acacatgtta aaacctataa
     8761 ttaattgcta ctaaaatatt aatgaattga aatggtgtct ctttatggca attaacaata
     8821 cagcctcgcg acgattactc gtcacgctaa cagccctttt tgcagcgctt tgcgggctgt
     8881 atctactcat tggcggaggc tggctggtcg cgattggcgg ctcctggtac taccctatcg
     8941 ctggccttgt gatgctcggc gtcgcctgga tgctgtggcg cagtaaacgc gccgcgcttt
     9001 ggctatacgc agccctgctg ctcggcacca tgatttgggg cgtctgggaa gttggtttcg
     9061 acttctgggc gctgactccg cgcagcgata ttctggtctt cttcggcatc tggctgatcc
     9121 tgccgtttgt ctggcgtcgc ctggtcattc ctgccagcgg cgcagttgcc gcactggtgg
     9181 tcgcactgct gattagcggt ggtatcctga cctgggccgg atttaacgat ccgcaggaga
     9241 tcaacggcac cttaagcgcc gatgccacac ctgccgaagc tatctccccc gttgccgatc
     9301 aggactggcc tgcgtatggc cgcaatcagg aaggtcaacg cttttcacca ctgaagcaaa
     9361 ttcacgccga taacgtccac aagctgaaag aagcctgggt gttccgtact ggcgatgtga
     9421 agcagccgaa cgatccgggt gaaatcacca atgaagtgac gccgattaaa gtgggcgata
     9481 ccctttacct gtgtaccgct caccagcgcc tgtttgcgct tgatgccgcc agcggcaaag
     9541 agaaatggca ttacgatcct gagctgaaaa ccaacgagtc tttccagcac gtaacctgcc
     9601 gtggcgtctc ttatcatgaa gccaaagcag aaacggcttc gccggaagtg atggcggatt
     9661 gcccgcgtcg tatcattctt ccagtgaatg atggtcgcct gattgcgatt aacgctgaaa
     9721 acggcaaact gtgcgaaacc ttcgccaata aaggcgtgct caatctgcaa agcaatatgc
     9781 cagacaccaa accgggtctg tatgagccga cttctccgcc aattatcacc gataaaacca
     9841 tcgtgatggc cggttcagtc accgataact tctcaacccg cgaaacgtct ggcgtgatcc
     9901 gtggttttga tgtcaacacc ggggagctgc tgtgggcttt tgatccgggc gcgaaagatc
     9961 cgaacgcaat cccgtcagac gaacacacct ttacctttaa ctcaccaaac tcgtgggcac
    10021 cagcggccta tgacgcgaag ctggatctgg tctatctgcc gatgggcgtg accacgccgg
    10081 atatctgggg cggtaaccgc acaccggaac aggaacgtta tgccagctcg attctggcgc
    10141 tgaatgccac taccgggaaa ctggcgtgga gctaccagac cgttcaccac gacctgtggg
    10201 acatggatct tccggcacag ccgacgctgg cggacatcac cgttaatggt cagaaagtgc
    10261 cgattattta cgctccggcg aaaaccggta acatttttgt gctcgatcgt cgtaatggcg
    10321 aactggtggt tccggccccg gaaaaaccgg ttccccaagg tgctgccaaa ggcgattacg
    10381 ttacccctac tcaaccattc tcggaactga gcttccgtcc gacgaaagat ttgagcggtg
    10441 cggatatgtg gggagccacc atgtttgacc aactggtgtg ccgcgtgatg ttccaccaga
    10501 tgcgctatga aggcattttc acgccgccat ctgaacaggg tacgctggtc ttcccgggta
    10561 acctggggat gttcgaatgg ggcgggattt ccgttgatcc aaatcgtgaa gtggcaattg
    10621 ccaacccaat ggcactgccg tttgtttcga aactgctccc gcgtggtcct ggcaacccga
    10681 tggagcagcc gaaagatgcc aaaggcacgg gtacggaatc cggcattcag ccacagtacg
    10741 gtgtaccgta tggtgtcacg ctcaacccgt tcctctcacc atttggtctg ccatgtaaac
    10801 agccagcatg gggttatatc tcggcgctgg atctgaaaac taatgaagtg gtgtggaaga
    10861 aacgtattgg tacgccgcag gacagtatgc cgttcccgat gccggttccg gtgccgttca
    10921 atatgggtat gccgatgctg ggcgggccaa tctccacggc gggtaacgtg ctgtttatcg
    10981 ccgctacggc agataactac ctgcgcgctt acaacatgag caacggtgaa aaactgtggc
    11041 agggtcgttt accggcgggt ggtcaggcaa caccgatgac ctatgaagtg aatggcaagc
    11101 agtacgtggt gatctccgca ggcggtcacg gttcatttgg tacgaagatg ggcgactata
    11161 ttgtggctta tgccctgccg gatgatgtga aataaccata atgcccggag agatccgggc
    11221 atatttccga atacggtctt tttataccgt aaaccctaac atcatccccg tatcttcatg
    11281 ctccagcaga tggcagtgcg ccatataagc atgttctttc ggtgcgttgt gattaaactt
    11341 caccagcact tcactgacat tgccttctac cttaacggta tctttccagc ccgcgcgatg
    11401 agccgctggc ggtttgccat tttctgacaa gatacggaac tgcgtgccgt gaatatggaa
    11461 cggatgcagc atcatgtcgc ccacgccaga gataacccaa cgttcgtact gccctttcgc
    11521 cgccgcaaac atcggcttat tcatatcaaa cgcctgaccg ttgattttat tggcatggtg
    11581 gaaatcgaac ttcccgccgt ggttcatatg attcatattg ccgtgcccca tatggcccat
    11641 catctggctg tgatccattc cggccatcgc ctgatcgcca tatttctcca ttagcatctg
    11701 catccccatc atatcgagca tcgggtccat agaaagttgc agcttgcgta ccgtcagccc
    11761 ttccagcgaa ggtaacgcag gcaggctact taatgtgtca ggcaaagcac cggaggcact
    11821 aatagcaatc ggctgaatcc gcattaccgg atgcggcttg tcaaacggtg caatcgccat
    11881 ccccatctgg ctgaccggca gcgtcaccag gtcaaagggt ttgttgtcgt taacctccac
    11941 cagcacttca aaacgctcgc ccatcagcac cggcagctcg ctcaccttca ccggttcagg
    12001 tagcagacca ccgtcgctgg caatcacata cagcgggcga ttgtcgctgg tggcgaaatt
    12061 gagcgagcgg gcattacagc cattgagcaa acgcaggcgc agccaaccac gcggggcagc
    12121 gtgttgtggg tagattgcac cgttggtcag cagcgtatcg ccaaaccagc ccacggcggc
    12181 ggtcatcaca tccagttgat aatcaatctg cccgtcggcg ctaaatttct tatcctgaac
    12241 gatcaccgga acatcatcga taccccactg ttttggcagc attaatttca ggatctcgtc
    12301 atcttcaatc accaccagac cagccagccc catcgccacc tgtcgcccgg tcttgccatg
    12361 ttgatgtgga tggaaccagc aggtagcggc aggttgatca acgttcaacg tcaccgagcg
    12421 cttgccacct ggcggaataa ttccctgtgt gccgccgtcg acgtcacccg gtacttccag
    12481 cccgtgccag tgcaacgtcg tctcttccgc cagttggttg tagatatcaa ccgttaccgc
    12541 tttgccgcgc tgtaatttca ccgccggccc cagcagattg ccgttatagc cccaagtagt
    12601 tgcagttttc tcgccaaagg tggactgacc tgcgccaata gttaactgaa tgcgattacg
    12661 ggcatcggtc gtgagcaaat cagggatcgg taacgttggg cgttccgccg caaagactgc
    12721 acggctccac agtggcaagg ctgaagccac acccagtgcg acggaatatt tcaagaaatc
    12781 acgacgttgc atagttattt ccttattctt aagcaggcga aacaaaatca aacgttgagc
    12841 atagtccttc cccttacggg aaggtcaagc cgcagacaca atttaatcga caataatggt
    12901 cgtcgcctcg ctcgcagtgt gctaacgttt atcttcttta agcccctggt agaagcaatg
    12961 aagacgtttt tcagaacagt gttattcggc agcctgatcg ccgtttgcgc aaacagttac
    13021 gcgctcagcg agtctgaagc cgaagatatg gccgatttaa cggcagtttt tgtctttctg
    13081 aagaacgatt gtggttacca gaacttatct aacgggcaaa ttcgtcgcgc actggtcttt
    13141 ttcgcccagc aaaaccagtg ggatctcagt aattacgaca ccttcgacat gaaagctctc
    13201 ggtgaagaca gctaccgcga tctcagcggc attggcattc ccgtcgctaa aaaatgcaaa
    13261 gccctggccc gcgattcctt aagcctgctt gcctacgtca aataatccct gatacctttt
    13321 tgtagaaata ttgaccgtgc atccgcggtc aatgttagct attatgttgc gccctttttt
    13381 tacgggtgtt aacaaaggag gtatcaaccc atggccgaaa aaaaacagtg gcatgaaacg
    13441 ctacacgacc agtttgggca gtactttgcg gtagataacg ttctgtatca tgaaaagacc
    13501 gatcaccagg atctgatcat ttttgagaac gctgcatttg gtcgcgtaat ggcgctggat
    13561 ggcgtagtac aaaccaccga gcgcgacgag tttatctatc atgagatgat gacccatgtt
    13621 ccgctactgg cccacggtca cgcgaaacat gtgctgatta tcggcggcgg cgacggtgcc
    13681 atgctgcgtg aagtaacccg acataaaaac gttgagtcaa tcacgatggt ggaaatcgat
    13741 gcgggtgtcg tgtcgttctg ccgtcagtat ctgcccaacc ataacgccgg tagctacgac
    13801 gatccgcgct ttcaactagt gatcgacgat ggcgtcaatt tcgttaatca aaccagccag
    13861 acctttgatg tcattatctc cgactgcacc gatcctatcg gtcccggcga aagcctgttc
    13921 acctcagcat tttatgaagg ctgcaaacgt tgcctgaatc ctggcggtat cttcgtcgca
    13981 caaaacggcg tctgcttttt acagcaggaa gaagccatcg acagccatcg caaactcagc
    14041 cattacttca gcgacgttgg cttttatcag gcggcgatcc cgacctatta cggcggtatc
    14101 atgacttttg catgggcgac agataacgac gccttacgcc atctctcaac cgaaattatt
    14161 caggcgcgtt ttctcgcttc tggcctgaaa tgccgttatt acaatccggc aatccatacg
    14221 gcagcttttg ccttacctca gtatctgcaa gacgcactgg cttcacagcc gtcctaagga
    14281 gaagataaga aattgaaaaa actgaaactg catggcttta ataatctgac caaaagtctg
    14341 agtttttgta tttacgatat ctgctacgcc aaaactgccg aagagcgcga cggttatatt
    14401 gcttatatcg atgaactcta taatgccaac cgtctgaccg aaatcctgtc agaaacctgt
    14461 tccattatcg gggccaatat tcttaacatc gcccgccagg attacgaacc acagggtgcc
    14521 agcgtcacta ttctggtgag tgaagaaccg gttgacccga aactcatcga caaaacagaa
    14581 caccccggcc cactgccaga aacggtggtt gctcatctcg ataaaagtca tatttgcgta
    14641 catacctacc cggaaagtca tcctgaaggc ggtttatgta ccttccgcgc cgatattgaa
    14701 gtctctacct gcggcgtgat ttctccgctg aaggcgctga attacctgat ccaccagctt
    14761 gagtccgata tcgtaaccat tgattatcgc gtgcgcggtt ttactcgcga cattaacggt
    14821 atgaagcact ttatcgacca tgagattaat tcgattcaga actttatgtc tgacgatatg
    14881 aaggcgctgt atgacatggt ggatgtaaac gtctatcagg aaaatatctt ccataccaag
    14941 atgttgctta aagagttcga ccttaagcac tacatgttcc acaccaaacc ggaagattta
    15001 accgacagcg agcgccagga aattaccgct gcgctgtgga aagaaatgcg cgagatttat
    15061 tacgggcgca atatgccagc tgtttaacgg ctctggcgga gctcccaggc tccgccagat
    15121 ttatttactt ctgctgcacg aaattgcggt aagccgccac gacctgcaga aaatcctcaa
    15181 cgccgcatag cgacaggctt tcttcgtcgt agtagttcat cccctcttcc atttcatcgc
    15241 cagcgaattc cagttgattg gcgcgaacca tcacctcttc agcgtccatc cacagggtgt
    15301 attcatgccc tgcccgttgc caggaccgtt cgctaccttt cagtgcgtgc gctgcttgtt
    15361 ccacttcatc aagcaaggcc aggttttctt tcacctcttc attaaaccag tgcccgacca
    15421 cttcatgccc catggacata cgcaccttta ccactccggt aatatcgcgc agaaattcgt
    15481 aatccatagt gtgttcctct gctcccggca atgcgccgaa atcgcatcgc gccattgatg
    15541 taattatcgc agccttaccg gagaaaaaca gcggaacggc gatagtttag aaataaaaaa
    15601 tgcccgggaa tctcattcac cgggcattgt gtcgtttatg cgcagcgcgt gcgcttactt
    15661 attacaccgc agtctggaaa atcaccccat cggctttttc ggtgtactga gaaagctggt
    15721 tgaagttcag ataacgataa gtatcaacgg cagttttatc tacttgcgca acgtaggtct
    15781 ggtactcttc cggcgtcggc agtttgccaa tcagcgccgc aacagccgcc agttctgcag
    15841 aagccaggaa gacattcgcg ccagtaccca aacggttcgg gaagttacgg gtagaggtgg
    15901 aaaccaccgt cgcaccgtct gctacacgcg cctggttacc catacacagg gaacagcccg
    15961 ggatctcgat acgagccccg ctcttaccga agacgctgta gtagccttct tccgtcagtt
    16021 gcgcggcgtc catacgggtt ggcggtgcca cccacaggcg ggtcggcaac tgacctttgt
    16081 gcgcatccag cagtttacca gcggcacgga agtgaccgat gttagtcatg caagaaccga
    16141 taaatacttc gtcgatcttc tcgccctgta ccgcagacag cggacgtgcg tcatccggat
    16201 cgttcggagc acacaggatt ggctctttaa tctccgccag atcgatgtcg atcactgccg
    16261 cgtattccgc atctgcatcg gcttccagca gctcaggatt cgccagccat ttttccatgc
    16321 cctgaatacg gcgttccagg gtacgacgat cgccgtaacc ttccgcgatc atccacttca
    16381 gcaggacgat gttggagctc aggtattcga tgatcggttc tttgttcagc ttgatagtac
    16441 aaccagcggc agaacgctcg gcggacgcat cggttagctc aaaggcctgc tcaactttca
    16501 gatccggcag accttcaatt tccagaatgc gaccagagaa gatgtttttc ttgcctttct
    16561 tctcaacggt cagcagacct tgtttgatcg catacagcgg aatggcgtgt accagatcgc
    16621 gcagggtgat gcccggctgc attttgcctt tgaagcgcac cagaacggat tccggcatat
    16681 ccagcggcat tacgccagtt gcggcagcaa acgccaccag accagaaccc gccgggaaag
    16741 agataccgat cgggaaacgg gtatgggagt caccaccggt accgacggta tccggcagca
    16801 gcatacggtt cagccaggag tgaatgacgc cgtcacccgg acgcagcgac acaccgccac
    16861 ggttcataat gaagtccggc agcgtgtggt gcgtgttcac gtcaactggc ttcggatacg
    16921 ccgcggtgtg gcagaaagac tgcatcacca ggtcagccga gaagcccagg cacgccaggt
    16981 ctttcagttc atcacgggtc atcgggccgg tggtgtcctg agagcctaca gaagtcattt
    17041 tcggttcgca gtacgcgccc ggacgaatgc ctttcacgcc acaggcacgg cctaccattt
    17101 tctgcgccag cgagaagccg cgatcgctct cagcgacatc tttcgcctga cggaacacat
    17161 cactgtgcgg cagaccaagt gcttcacgcg ctttggtggt caggccacgc ccgataatca
    17221 gcggaatacg gccgccagca cgcacttcat caatcagcac gtcggttttc agttcgaagg
    17281 tcgccagcag ttcgcctgtt tcgtggttac gcacttcacc tttgtacggg taaacgtcaa
    17341 tcacgtcgcc catgttcagg ttagagacgt tgacttcgat tggcagtgca cccgcgtctt
    17401 ccatcgtgtt aaagaagatg ggtgcaattt taccgccgag gcacaaacca ccgccgcgtt
    17461 tgttcggcac atgtggaata tcatcgccca taaaccacag aacggagttc gtggcggatt
    17521 tacgcgatga acccgtaccc acaacgtcac cgacgtacgc cagcgggaaa cctttctgtt
    17581 gcagagcttc aatctgtttg atcgggccaa caacacctgg ctggtctggc tcaatacctt
    17641 cacgggcgtt tttcagcatc gccagcgcgt gcagtgggat atccgggcgt gaccacgcat
    17701 ccggtgccgg agaaaggtca tcggtgttgg tttcgccagt gactttaaag acggtaacgg
    17761 tcagtttttc agccagcgcc gggcgattca ggaaccattc ggcatccgcc caggactgca
    17821 ttacctgctt cgcatattcg ttgcctgctt tcgctttctc ttctacgtca tagaagttat
    17881 cgaacatcag cagcgtgtga gaaagtgctt tggcagcaat aggtgccagt ttggcatcat
    17941 ccagcgcgtc gatcagcgga tgaatgttgt aaccaccctg catggtgccc agcagttcga
    18001 tagctttttc cggagtcagc agaggggatt tagcttcgcc tttagcgata gcagccagga
    18061 agcctgcttt tacataggcg gcttcatcga cgcctggggg aacacggttg gttaacagat
    18121 ctaacaggaa ttcttcttcg cccgcgggcg ggtttttcag cagctctaca agcgcggcca
    18181 tttggtttgc atccaggggt ttgggcgcaa tcccctcagc ggcacgctca gctacgtgct
    18241 tacggtattc ttctagcacg acggttctcc tcgctctcat tgtcatagtg cggcaggcga
    18301 ttctcttcac gctcctgtga gacagcagtt tgtagggtaa atgcccagta ccgcaacggg
    18361 cagaatagca ggattttgac gacgtgttaa tctgtttaca aaaaagcaac ataaaaaagt
    18421 ggctgaatcg ttaaggatgg tctagagatt gtttccctcc gtaaaaactt cgttaattcg
    18481 catggtgata atcacctttc tcaacgaatc ccaagattag tcaaaattta aactaccgcc
    18541 tctttatact cggattcaca gcacctgcgg gtggcagttc gccgaccatt gcgatttcct
    18601 tgagatccga attatgaaaa tgactttgcc gtttaaaccc catgtgctgg cgctaatttg
    18661 cagtgccggg ctttgtgctg cctctgccgg gctatatata aaaagccgca cagtggaagc
    18721 gcctgtagaa ccgcaatcga cacaactggc tgtgtctgac gctgccgctg ttacgcttcc
    18781 tgcaacggtt tccgcacctc ccgtaatacc cgcagtcgtc aaatccgcat tcagcactgc
    18841 acaaatagat caatgggtcg cacccgtcgc gctgtatccc gacgccctac tttcgcaggt
    18901 gctgatggca tcaacctatc cgacaaacgt tgctcaagca gtgcaatggt cgcacgataa
    18961 tccacttaaa caaggcgatg ctgctattca ggcggtatct gaccagccgt gggacgccag
    19021 cgttaaatca ctggtggcct ttccacaatt gatggcattg atgggcgaaa acccgcaatg
    19081 ggtgcaaaac ctgggcgatg cttttctggc gcagccgcag gacgtgatgg actcggtaca
    19141 gcgattgcgg caactggcgc aacaaaccgg ctcgctgaag tcatcaaccg aacagaaagt
    19201 tattaccaca acgaagaaag ctgtaccggt aaaacagaca gtcacggcac ccgtcatacc
    19261 atccaatacc gttttaactg ccaaccccgt cattacagag ccggcaacaa ccgtcatttc
    19321 cattgagccc gccaatcctg atgtggtcta tattcccaac tacaacccaa ccgtggttta
    19381 cgggaactgg gccaatactg cgtatccgcc ggtttatctg ccaccaccag ccggagaacc
    19441 gtttgttgac agctttgtgc gcggattcgg ctatagcatg ggcgttgcta ccacgtacgc
    19501 actattcagc agcatcgact gagatgacga cgatcatgac catcatcatc atgacgatga
    19561 tgattatcat caccacgatg gcggtcatcg tgacagtaat ggctggcaac acaacggcga
    19621 caacatcaat atcgacgtca acaatttcaa ccgtatcacc ggtgagcatc ttactgataa
    19681 gaatatggca tggcggcaca atccaaacta ccgtaatggt gtgccctatc atgatcagga
    19741 tatggcaaag cggtttcatc aaaccgatgt caacggcgga atgagtgcca cgcagctacc
    19801 tgctccaaca cgcgacagcc agcgtcaggc ggcagcaagt cagtttcagc aacgaacaca
    19861 cgccgccccc gtcattacac gagataccca acgtcaggca gcggcacagc ggtttaatga
    19921 agctgaacac tatgggagct atgacgactt ccgcgacttc agccgtcgcc aaccactgac
    19981 ccagcagcaa aaggacgccg ctcgtcagcg ttatcagtcg gcctctcctg agcagcgcca
    20041 ggcagtccgc gagaaaatgc agactaaccc gcagatccag cagcgaaggg aggcggcgcg
    20101 ccagcgtatt cagtccgcct cgcctgagca gcgccaggcg gttcgcgaga aaatgcagac
    20161 taacccgcag aaccagcagc gaagagaggc agcgcgtgag cgtattcagt ctgcaactcc
    20221 cgaacagcgc caggtgttta aggaaaaagt acagcagcgc ccactgaacc aacagcaacg
    20281 tgataacgcc cgccagcgta tacaatcggc atcacctgaa caacgtcagg tttttcggga
    20341 gaaagttcag gagagccgcc cacaacgtct aaacgacagt aaccatactg tcaggctgaa
    20401 taacgagcaa cggtcagcag tacgcgaacg tctctctgaa cgtggagcaa gaagactgga
    20461 aaggtaaatt gcagacgtaa aaaaagcggc gtggttagcc gcttttttaa ttgccggatg
    20521 ttccggcaaa cgaaaaatta cttcttcttc gctttcgggt tcggcaggtc ggtaatgcta
    20581 ccttcgaaca cttctgccgc caggcccaca gactcgtgca gagtcgggtg cgcgtggatg
    20641 gtcagtgcga tgtcttcagc atcacaaccc atttcgattg ccaggccgat ttcacccagc
    20701 agctcgccgc cgttggtacc aacaatcgca ccaccgatca cacggtgaga ttctttgtcg
    20761 aaaatcagct tggtcatacc gtctgcgcag tcagaagcga tagcacgacc agaagcagcc
    20821 cacgggaagg tggcggtttc atagctgatg cctttctctt tcgcttcttt ctcagtcaga
    20881 cctacccatg caacttctgg ttcggtatag gcgatggacg ggataacttt cggatcgaag
    20941 tagtgtttct taccggcgat aacttcagcg gcaacgtgac cttcgtgaac acctttgtgt
    21001 gccagcatcg gctgaccgac gatatcgccg atagcaaaga tgtgcggtac gttggtacgc
    21061 agctgtttgt caacgcggat gaaaccacgg tcgtcaactt ccacgccagc tttgcctgcg
    21121 tcgaggtttt taccgttcgg cacacgacca atcgctacca gcacggcgtc gtaacgctgc
    21181 ggttcagctg gtgctttttt gccttccatc gtcacataaa taccgtcttc tttcgcttca
    21241 acggcggtaa ctttggtttc cagcatcagg ttgaatttct tgctgatacg cttggtgaag
    21301 actttaacga tgtctttgtc agctgccggg ataacctggt cgaacatttc aaccacgtca
    21361 atctgtgaac ccagcgcgtg gtaaacggtg cccatttcca gaccgatgat accgccaccc
    21421 attaccagca ggcgttctgg tacttctttc agttccagcg cgtcagtgga gtcccagata
    21481 cgcggatctt catgcggaat aaacggcagt tggatcgggc gagaacccgc tgcaatgatc
    21541 gcattgtcga agttgatcac ggttttgccg ttctcacctt caacttccag ggtgttagcc
    21601 ccggtgaatt tacccagacc gttgaccact ttgactttgc ggcctttcgc cataccagcc
    21661 agaccaccgg tcagctgatt gatcactttc tctttccagg tacgaatctt gtcaatatcg
    21721 gttttcggtt cgccgaagac gataccgtgt tcagccagcg ctttggcttc ttcgataact
    21781 tttgctacgt gcagcagtgc tttagaaggg atacagccga cgttcaggca aacaccgcca
    21841 agggtgttgt aacgttctac gattacggtt tccagaccta aatcagcgca acggaaggca
    21901 gcggagtaac ctgcggggcc tgccccaagt accacgacct gagttttgat ttcagtactc
    21961 atcatgacct ctatatattt atctccggcg gtcatacccg tcgtctttca ggccgcagcg
    22021 gcgttagctt cacgccctca ccccagtcac ttacttatgt aagctcctgg ggattctggc
    22081 gcttgctacc ttgctgcacc cagaaatcca tagggtatgt gaagtaatac cctaaccacc
    22141 accgggtcgt tctatccgtc ggtattttac aaaattgtta acaattttta aacaacaaac
    22201 ggcaaccgat ttgtctattc gctaataacc tcaatacatt catgagatta ccagaaaaaa
    22261 gccggccgtt gggccggctc ttttacttac atcaccagac ggcgaatgtc agacagcgtg
    22321 ttgttaatga tggtaatgaa acgggcacca tcagcaccgt cgatcacgcg gtggtcgaag
    22381 gagagagaaa tcggcagcat cagacgcggc acgaactctt taccattcca caccggctcc
    22441 atcgcggact tggaaacgcc gaggatagcc acttccggcg cgttcacaat cggcgcgaag
    22501 tgggtagtac ccaggccgcc gatgctggag atggtgaagc aaccgccctg catttcgccc
    22561 gcagtcagct taccgtcacg cgctttctta gaaatagtca tcagctcgcg agacagctcg
    22621 atgatgcctt tcttgttgac gtctttgaat accggaacaa ccagaccgtt cggggtatcc
    22681 accgccacac cgatgttgat gtatttcttc agggtcagac gctgaccgtc ttccgacagc
    22741 gaactattga agcgaggcat ctgctcaaga gctgcagcaa cggctttcat gatgaagaca
    22801 accggggtga tcttcacatc cagcttacgt ttcgccgctt cttcgttctg ctgtttacgg
    22861 aacgcttcca gctcggtgat atcggttttg tcgaagtggg taacatgcgg gatcatcacc
    22921 cagttacggc tcaggttagc accagaaatt ttctggatac ggcccagttc cacttcttcg
    22981 atttcgccaa acttgctgaa gtccaccttc ggccacggca gcatgccagg gataccaccg
    23041 ccagtcgccg ccggagctgc ttctgcacgt ttgatagctt ctttcacgta agcctgaacg
    23101 tcttcgcgca ggatacgacc tttacggcca gtgcccttca ctttcgccag gttaacgcca
    23161 aactcgcgtg ccagacggcg gatcagcggg gtcgcgtgaa cgtaagcgtc gttttcagca
    23221 aattcagatt tgccttccgc tttcgcagcc ggagcttcag cttttgctgc cggagccggc
    23281 gctgccgctt cctgtttcgc aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc
    23341 ataatcagcg aaccagtttt cactttatcg ccaacgttga ctttcagttc cttcacgacg
    23401 cctgcaaacg gagccggaac ttccatagaa gctttatcgc cttctacggt aatcagtgac
    23461 tgttcagcgg caactttgtc gcccactttc accatcactt cggtcacttc aacttcgtca
    23521 ccgccgatat ccggaacgtt aacttctttc acgccagctg ctggtgcagg ggtcgctgcc
    23581 ggagtcgctt cctgtttagc cgccggagct gccgcgcctg cttcacccgc gacttcgaag
    23641 accataatca gcgagccggt agacactttg tcacccacgt tcactttgat ctctttcacg
    23701 gtacctgcaa acggagccgg aacttccata gaagccttgt cgccttctac ggtgatcagc
    23761 gactgttcag cttcaacttt atcgccaact ttcaccagga tttcggtcac ttcaacttcg
    23821 tcgctgccga tatccggaac gttaacgtct tttgccgccg cagccgcggg tgctgctgcc
    23881 ggagctgctt ctttcttctc ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg
    23941 gaatcgaaaa tcataatcag tgcgccggtc tgggttttat cgccaacaga gactttgatc
    24001 tctttaacga tacccgcctg cggagatgga acttccatag aggctttgtc gccttctacg
    24061 gtgatcagcg actgttcggc ttcaactttg tcgcccactt tgaccaggat ctcggtgatt
    24121 tcaacttcat cagccccgat gtccggtact ttgatttcga tagccattat tcttttacct
    24181 cttacgccag acgcgggtta actttatctg catcgatgtt gaatttggcg attgcgtcag
    24241 caaccacttt cttatcgatt tcgccacgtt tagccagttc gcccagcgcc gcaaccacga
    24301 cataagaagc atcaacttcg aagtggtgac gcaggttctc acggctgtcg gaacgaccga
    24361 agccatcagt acccagtacg cgatagtcgt cagccggtac gtaagtacgg acctgctcag
    24421 cgaacagttt catatagtcg gtagatgcca ctgccggcgc gtcgttcatc acctgagcga
    24481 tatacggaac gcgcggagtt tccagcggat gcagcatgtt ccagcgttca caatcctgac
    24541 catcacgcgc cagctcggtg aaggaggtca cgctataaac gtcagaacct acgccgtaat
    24601 ctttcgccag gatctctgcc gcttcacgga cgtgacgcag gatggaaccg gagcccagca
    24661 gctgaacttt acctttgcta ccttcaatgg tttcgagttt gtagatacct ttacggatac
    24721 cttcctcagc accttccggc attgccggca tgtggtagtt ttcgttcagc gtagtgatgt
    24781 agtagtaaac gttctcttgt ttttcaccgt acatacgctc cagaccgtca tgcataatga
    24841 cagcaacttc gtaagcgtaa gccgggtcgt aagagataca gttcgggata gtcagcgact
    24901 gaatgtggct gtgaccatct tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg
    24961 aagtaccgcc gatcaggaag ccacgcgctt gctggtctcc agccgcccag cacagatcgc
    25021 cgatacgctg gaagccgaac atcgagtaat agatgtagaa tgggatcatc ggcagattgt
    25081 tcgtgctgta agaggtcgcc gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc
    25141 cttcctgcag aatctgacct ttctcgtctt ctttatagta agcaacctgc tcgcggtcct
    25201 gcggggtgta ctgctgaccg ttcgggctgt aaataccaat ctgacggaac agaccttcca
    25261 taccgaaagt acgcgcttcg tcggcgatga tcggtaccag acgatctttg atcgacttgt
    25321 tcttcagcat cacgttcaga gcacgaacga aagcgatagt ggtagagagc tctttgctct
    25381 gctcttccag cagcgcgccg aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt
    25441 tcggctgacg gcttggcaga taaccgtgca gtttctgacg ctgagcgtgc agataggtat
    25501 gctcttcaga accttccggg aaggtgatgt acggcagttt ttcgatatct gcatcagaca
    25561 ccggcacatt gaaacggtcg cggatgtgac gcacgccgtc catgttcatt ttcttaacct
    25621 ggtgcgcgat gtttttacct tcagccgcgt cgcccatgcc gtaaccttta atggtatgag
    25681 caaggattac tgtcgctttg cctttggttt cctgcgcttt cttgaatgca gcgtagattt
    25741 tcttcggatc gtgaccacca cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa
    25801 ccagtgctgc ggtttcagga tatttaccga agaagtgttc acgaacgtac gcaccatctt
    25861 tcgatttgaa ggtctggtag tcgccgtcaa cggtttcgtt catcagctgg atcagtttac
    25921 cgctggtatc tttacgcagc agttcatccc aacggctacc ccacatcact ttgatcacgt
    25981 tccagccagc accttcgaag atgccttcca gttcgttgat gatcttgccg ttaccggtga
    26041 ccgggccgtc aagacgctgc aggttacagt tgataacgaa aaccaggtta tccagttttt
    26101 cacgggtagc gatggtgatc gcacctttgg attccggttc gtccatttca ccgtcgccga
    26161 ggaacgcgta aacggtctgt ttagaggtat ctttcaggcc acggtgttcc agatatttca
    26221 ggaatttagc ctggtaaata gcaccaatcg gacccagacc catagatacg gtcgggaact
    26281 gccagaattc cggcatcagt ttcgggtgcg gataggaaga gaggccattg ccgtgaactt
    26341 cctgacggaa gttatccagc tgctcctgag tcagacgacc ttccaggaaa gcacgggcgt
    26401 aaacgcccgg ggagatgtgg ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc
    26461 gtgcacggaa gaagtggtta aagcacacat cataaatggt tgcggaagac tggaaggacg
    26521 ccatgtggcc gcccagttcg aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt
    26581 tccagcggat agctgaacga atacggcgtt ccagttccag attacccgga tactccggtt
    26641 gttcttcaac ggggatggtg ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta
    26701 caccgccttt gcgggcttca gcaagcagtt ggtcgatcag atactgagca cgctcaacac
    26761 cttcttcacg gatgaccgat tcgatcgcct ggagccagtc gcgagtttcg atcggatcca
    26821 cgtcatttgg gaaacgttct gacatgggtt attccttatc tatctaataa cgttgagttt
    26881 tctggaacct gtcccattga actctcgcca gaaagctcaa taagacaggt tctacgttta
    26941 gttaccgcgc ttttatatgc gcttgattta caacatcttc tggataattt ttaccagaaa
    27001 aatcactaat tctttcgttg ctccagacga cgcagagaac gctcacggcg gctctcttca
    27061 cggcttctgt cgagcaaaat ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc
    27121 gcttcttccg gcttaccgac cataatcgct tcaaatatgc gggtgcggtg actactcacc
    27181 agcggcagca tctcgcgacg cgaatagagc aattcgaagt tctggcggac attctgggcc
    27241 aacatcggct ccatacacct tagcagatga agcagaacca cattgtgggc cgcttcggtg
    27301 acggcaatct gatactggag tacggcgttt gattccgcgt ccagatcgcc agactgctgc
    27361 gccagctcta tggcgtggtg gagttcacgg atgcgttcct tgtcttcatc ggtactacgc
    27421 agcgcggcgt aataagcggc gataccttcc agggcgtgtc gtgtttcgag caagtcatac
    27481 tgtgattcag gatggtcgga gagcagctcc accagcggat cgctgaagct ttgccatagg
    27541 ctgctctgga caaaagtgcc gccaccctgg cgacgaagca acaagccctt cgcttcgaga
    27601 cgttgaatcg cctcacgcaa ggagggacgg gagacgtcga actgttttgc cagttcgcgt
    27661 tccggtggga gtttttcgcc cgggcggaga gtgccttcga ggatcaaaaa ctccagttgc
    27721 tgctcaatca catcggagag ttttggttgg cggattttgc tgtaggccat gagttcctgt
    27781 cttaagccac ttgccgaagt caattggtct taccaatttc atgtctgtga cgctaaagta
    27841 acaaagtatt caccttatgt ccatacaggt tttgattgaa atcatgaaac tgtgcacatt
    27901 ttaacaactt gacatatata acgtttcaaa gttgtaacta tgcacaaatg tagactttac
    27961 gtaggaaagg gattttttaa cgattattaa actataaaaa tccgaattga accgattcac
    28021 ttaccaattt ttttgatttt taagtcaata aaaacgaatt taaattcact ctgcatattg
    28081 agaggggttg aggctgagct ttacaaacgg tttcttttta agcaactcat cttcaaccat
    28141 gcataaagcg ggtgcattcg ctgccgcata ccattattct tgatctgacg gaagtctttt
    28201 tgtaacaatt caaacctcct tgatgtaaac aaatcaatac aacaaacgga attgcaaact
    28261 tacacacgca tcactgcgta gatcaaaaaa acaatcaccg cacgaggttt catgatggaa
    28321 ggtcaacagc acggcgagca gctaaagcgc ggccttaaaa accgccatat tcagcttatc
    28381 gcgctgggtg gcgcgatagg gacagggcta ttcctaggta gcgcctccgt aatacagtcc
    28441 gcagggccag ggattatcct gggttacgcc attgctggtt ttatcgcctt tctgatcatg
    28501 cgtcagctgg gtgaaatggt ggtcgaagaa cctgtcgcag gctcctttag ccactttgct
    28561 tataaatact ggggcagttt tgccggtttc gcctctggct ggaactactg ggtactgtac
    28621 gttttagttg ccatggcaga gctgactgcc gtgggtaaat acattcagtt ctggtatccg
    28681 gaaatcccaa cctgggtttc tgccgccgta ttctttgtgg tgattaacgc catcaacctg
    28741 accaacgtta aagtgtttgg cgagatggag ttctggtttg ccattatcaa agttattgcg
    28801 gtagtagcga tgatcatctt cggcggctgg ctgctgttca gtggcaacgg cggcccgcag
    28861 gcgagcgtta gcaacctgtg ggatcaaggt ggtttcctgc cgcacggctt caccggactg
    28921 gtgatgatga tggcgattat catgttctcg ttcggtggtc tggaactggt ggggatcacc
    28981 gcagcagaag ctgataaccc ggagcaaagt atcccgaaag caactaacca ggttatctac
    29041 cgcatcctga ttttctatat tggttcgtta gccgttctgc tctcactgat gccgtggacc
    29101 cgcgttaccg ccgataccag tccgtttgtg ctgatcttcc acgagttagg cgataccttt
    29161 gtggcgaatg cgctgaacat cgtggtactg actgcggcgc tctccgtgta caacagctgc
    29221 gtatattgca acagccgtat gctgtttggt ctggcacaac aggggaacgc gccaaaagcg
    29281 ctggcgtctg tcgataaacg cggcgtaccg gttaacacca ttctggtgtc tgcgctggtt
    29341 acagcattgt gcgtattgat taactatctt gctccggaat ccgcatttgg cctgttaatg
    29401 gcactggtgg tatccgcact ggtgatcaac tgggcgatga tcagtctggc gcatatgaag
    29461 ttccgtcgcg ccaagcagga acaaggcgtg gtaactcgct tccctgctct gctttatccg
    29521 ctgggtaact ggatctgcct gctgtttatg gcggcggtac tggtgattat gctgatgacc
    29581 ccaggaatgg cgatttcggt atacctgatc ccggtatggc tgatcgtgtt aggtatcggc
    29641 tatctgttta aagagaaaac cgccaaagcc gtaaaagcgc attaatctct ctacgccctc
    29701 acccgtacag ggtgagggca ataatctcta caccaacgcc ccgtaaatcg tcagtagcgc
    29761 aatcaccacc acgaccacga acgaggtttt cttcgccatt gaaaccgctg ccttcggcgt
    29821 ctccacctta tcgacatgcg gttcacgcgc cagagagaac tgcgccagac gcgttaatac
    29881 ctgatattgc gaagtatgga aatcacccag cgaagcaaac caggccggta acgctttctc
    29941 accatgaccg atcaaggcat ataccacacc cgcaagacga accggcaccc aatccagtac
    30001 atgaagcact gcatcaatgc cggactgtaa acgatgatgc ggcgtctggt atcgcgccag
    30061 ccagtattgc catgcacgca agaacgcata ccccatcagc gtaacgggtc cccaggttcc
    30121 ccccacaatc agccagaaca gcggtgcaag ataaaaacga aagttaatcc acagcaatgc
    30181 attttgcagc tcacgcaaat actcacgttc gtcgcagcct gccgggacgc cgtgaatcat
    30241 ggtgagttcg ccagccatcg tggcacgggc atggctatca ttacgtgaag cagctgtcag
    30301 ataagcatga taatgaagac gaactttacc tgcgccaata cacagcaaac caatcagcag
    30361 ccacaccagc agcgtgggaa cgttgaacaa tactccctgc aatgcgcgta acagtaaaaa
    30421 agtcacaccc atcgcaataa tggtcatgcc taacgtgcgc ccgagagaaa aatgtttcac
    30481 ccgccgaaag aacgcttcaa gacgatgatc aagctgccag tgctcgccca acttaaacag
    30541 gcgctcgaaa attaacacca gtaaggttgt aaatagcgtc atgttgtctc cttgctgacc
    30601 agcgcacgaa accgtgccca atcaaatgca ggaccgggat cggttttccg atccggcgca
    30661 atatcacaat ggcccgtcat gttattagcg atatcgggat agcgatctat cagtgcccgc
    30721 gtaaccgccg caagctgttg atactgcgca tcggtatacg ccagagtatc ggtgccttca
    30781 agctcaatcc caatagaaaa atcattgcag cgttcacgcc cctgatactg agagactccc
    30841 gcatgccagg cacgtttatc gaaaggaaca tactggacta tttcaccatc acggcgaatc
    30901 aaacagtgag cggagacgcg caaatgggcg atctcagcaa agaaaggatg tgcctgcgga
    30961 tcaatagttc cagtgaataa tgcgtcgatc cacggaccgc caaactcgcc tggcggcagg
    31021 ctaatattgt gcaccaccag cagggtgggt gtttcgtcat ccaggcggca atcgtaatgt
    31081 ggtgagggaa cgcggcgcgc gccaaccagc cacccctgtt ctaacaacat gcaggatctc
    31141 cttatatgtg gtgctaatac ccggttcaga gtagcatgtt tctaccttat gattcgttag
    31201 ctatctggag ttttaacatg ccgcctcgcc gctacaaccc tgacacccga cgtgacgagc
    31261 tgctggaacg cattaatctc gatatccccg gcgcggtggc ccaggcgctg cgggaagatt
    31321 taggcggaac agtcgatgcc aacaatgaca ttacggcaaa acttttaccg gaaaattctc
    31381 gctctcatgc cacggtgatc acccgcgaga atggcgtatt ttgtggcaaa cgctgggttg
    31441 aagaggtgtt tattcaactg gcaggcgacg atgtcaccat aatctggcat gtggatgacg
    31501 gcgatgtcat caacgccaat caacccttgt tcgaacttga aggcccatcc cgcgtgctgt
    31561 taacgggcga acgcactgcg cttaattttg tgcaaaccct ttcaggagtt gccagtaagg
    31621 tacgccacta tgtcgaatta ctggaaggca ccaacacgca gttgttggat acgcgcaaaa
    31681 ccttacctgg cctgcgttca gctctgaaat acgcggtact ttgcggcggc ggagcgaatc
    31741 accgtctggg gctttctgat gccttcctga tcaaagaaaa ccatattatt gcctccggct
    31801 cagtgcgcca ggcggtcgaa aaagcgtcct ggctgcatcc ggatgcgcca gtagaagtcg
    31861 aagtagagaa tctggaagaa cttgatgagg ccctgaaagc cggagccgat atcatcatgc
    31921 tggataactt cgaaacagaa cagatgcgcg aagccgtcaa acgcaccaac ggcaaggcgc
    31981 tactggaagt gtctggcaac gtcactgaca aaacactgcg cgaatttgcc gaaacgggcg
    32041 tggactttat ctccgtcggt gcgctaacta aacacgtaca agcactcgac ctttcaatgc
    32101 gttttcgcta attgctgaca gatttatccg ctccatcgcg ggcggataat ctccccttcc
    32161 ccaactttct tcgtaacgcc tcgcaaattt cttgtctttc gctgcaacga aataaaaaac
    32221 ctcggaatct gctttcccgt ttcgtttttt gcccctcgcc tgtgctgatg gcgtttgcca
    32281 aagtagcacc aaccaaatca aggagcgaaa cagatggaca agcaacgcgg ttttacactt
    32341 atcgaactga tggtggttat tggcatcatt gccattttaa gctccattgg tattcccgct
    32401 tatcaaaact acctgcgcaa agccgcactc accgacatgc tacaaacctt tgtgccttac
    32461 cgtaccgccg tagagttgtg cgcgctggaa catggtggat tagatacctg cgacggtggc
    32521 agcaatggca ttccctcgcc taccaccacc cgctatgttt cagccatgag tgtggcaaag
    32581 ggcgtggtgt cgctgaccgg gcaagaaagt ctcaatgggc taagcgtcgt catgacgcca
    32641 ggttgggata acgcaaacgg cgtcaccggc tgggcgcgca actgcaatat tcaaagtgac
    32701 agcgcattgc agcaagcctg cgaagatgtc ttccgctttg atgacgccaa ctaaggagcg
    32761 gcaatgaata ttccacagct cacggccctg tgcctgcgtt atcagggagt cttgctggat
    32821 gccagcgaag aagtggttca tgttgcggtg gtcgatgccc cctcacatga gttgctggac
    32881 gcattgcatt tcgctaccac caaacgtatt gagatcacct gctggacgcg ccaacaaatg
    32941 gaaggtcacg ccagtcgcac acaacagaca ttacccgtag ctgttcagga gaagcatcag
    33001 cccaaagcag agttgctgac tcgaacgtta caatctgcgc tggaacaacg cgcgtctgat
    33061 attcatatcg aaccagcgga caatgcctac cgcatccgct tgcgtatcga cggcgtattg
    33121 catcctttac cggatgtttc accggatgcc ggagtcgcat taacagccag attaaaagtg
    33181 ctgggaaacc tggatattgc ggaacatcgc ctgccgcagg acgggcaatt cactgtcgaa
    33241 ctggcaggaa acaccgtctc atttcgtatt gcgaccttac catgtcgggg tggtgaaaag
    33301 gtggtattaa ggttgttaca gcaggtgagc caggcactgg atgttaacac gctgggaatg
    33361 cagccgttac aactggcgga ctttgctcat gccttgcaac aaccacaggg actggtgctg
    33421 gtaactggcc ctacaggcag tggcaaaacg gtcacctggt gttgtccacg ctacacacca
    33481 attccaccac tgaaacgctg gtacgtttac agcaaatggg ggtcgcccgc tggatgctat
    33541 catcggcgct tacgctggta atagcccagc gtctggtacg taaactttgc ccacattgtc
    33601 gccgacagca aggggagccc atccatattc cagacactgt atggccgtcg ccgctgcccc
    33661 actggcaggc acccggttgt gtacattgct accacggttt ttatggtcgt accgccttgt
    33721 ttgaagtcct gcccattaca ccagccatac gccagcttat ttccgctaat actgaagttg
    33781 aatcgctgga aacgcacgcc cgacaggcgg gtatgcgtac gctttttgaa aacggctgcc
    33841 tggccgtgga gcaaggctta accacctttg aagagttaat ccgcgtactg gggatgccgc
    33901 atggcaagta agcaactctg gcgctggcat ggcataaccg gcgacggcaa tgcgcaagat
    33961 gggatgctat gggcagagag ccgtacttta ctgcttatgg cactacagca acagatggtt
    34021 accccactaa gcctgaagcg aatcgccatc aattctgcgc agtggcgagg agataaaagc
    34081 gcggaagtca ttcatcaact ggcgacgcta cttaaagccg ggttaacgct ttctgaaggg
    34141 ctggctctgc tggcggaaca gcatcccagt aagcaatggc aagcgttgct gcaatcgctg
    34201 gcgcacgatc tcgaacaggg cattgctttt tccaatgcct tattaccctg gtcagaggca
    34261 tttccgccgc tctatcaggc gatgatccgc acgggtgaac tgaccggtaa gctggatgaa
    34321 tgctgctttg aactggcgcg tcagcaaaaa tcccagcgtc agttgaccga caaagtgaaa
    34381 tcagcgttgc gttatcccat catcatttta gcgatggcaa tcatggtggt tgtggcaatg
    34441 ctgcattttg ttctgccgga gtttgccgct atctataaga ccttcaacac cccactaccg
    34501 gcactaacgc aggggatcat gacgctggca gactttagtg gcgaatgggg ctggctgctg
    34561 gtgttgttcg gctttctgct ggcgatagcc aataagttgc tgatgcgccg cccgacctgg
    34621 cttatcgcgc ggcagaaatt gctgttacgc atcccgatta tgggttcact gatgagggga
    34681 caaaaactca cgcagatctt tacgattctg gcgctgacac aaagtgcagg cattactttt
    34741 ttacagggcg tagagagcgt cagagaaaca atgcgctgcc cgtactgggt gcaacttctg
    34801 acacaaatcc agcacgatat cagtaacggt catcccatct ggctggcgct aaaaaatgcc
    34861 ggtgagttta gcccgctctg tttgcaatta gtgagaacag gagaggcatc cggctcgctg
    34921 gacctcatgt tagacaacct cgcccatcat catcgggata acacaatggc gctggcggat
    34981 aacctcgcag ccttactgga accggcgttg ctgatcataa cgggaggaat tatcggtacg
    35041 ctggtggtgg caatgtatct gccaattttc catttaggcg atgcgatgag tgggatggga
    35101 taacgctggc gtgttgctcc acgccagcgt tgggagatta caggttgttg aagatgcggt
    35161 tttcttgttc cagcacacga ataaacgtgg tgcgcttggt cagctctttc aggcgtgaag
    35221 ccccaacgta tgtacaagct gaacgcaggc cgcccaaaat atcacgcgcg gtattttcaa
    35281 cctggcctcg cagcggcagc ttaacggttt taccttctgc tgcgcggtat tccgcaacgc
    35341 cgccaacgtg acgtttcatc gcagactcgg agctcatgcc gtagaacagc ataaatttct
    35401 cgccgttctc ctcaacgatg cgaccgccgc tctcttcgtg gcccgccagc atgccgccaa
    35461 gcatgacgaa atcggcaccg ccgccaaagg ctttcgccac atcgcccggc gtggtacagc
    35521 caccgtcgct gacgatcatt ccgcccagac cgtgcgccgc atcggcacat tcgattaccg
    35581 cagaaagctg cggataaccg acgcctgttt tgacgcgagt tgtacaaaca gaacctgggc
    35641 caatgccaac tttaacgata tcagcaccgg agaggataag ctcctcacac atttcaccag
    35701 tcactacgtt gccagcacaa atggttttgg tcggccacgc ttcacgcgct ttcgcaacga
    35761 actgcacgaa gtgttcggaa taaccattcg ccacatcaat acaaacgaag tttaatgccg
    35821 ggttcaggtc gagaatctgt ttagtttttt cgaaatccgc atcagacgtg ccggtagaaa
    35881 ccatcacatg tttcagcaca tcagcggaag aattgttgat aaacgcttgc cactcttcga
    35941 cagaaaagtg tttatgcaca gcagtcagaa tatcaaaaga agccagcgca gaggccatag
    36001 aaaatgtgcc tacggtgtcc atatttgcgg cgataatcgg cacgccggac cagctctgac
    36061 ctgaatgttt gaaggtgaat tgacgttcca gttcaacatc ggaacggctt ttaagagtgg
    36121 agcgtttagg gcggatgaga acgtctttaa aacctaactt cagatcttct tcaatacgca
    36181 tgtgcggatt cctggggtta atggctataa gggtaagagc gcaactccag tgacgctatc
    36241 atacgcacta atcaatgcgg cgcaagactg cgaaattgtc tttttttacg ctacaatccc
    36301 ataaatttac ctggtaaata gtgagcgaac gtttcatcaa tttcactttc gcgcgctcat
    36361 ttttaacgtt ttgataatca tgatgaattc ccgggaatat aaattatggg gtatatagtt
    36421 gccttaacgg gaggcattgg cagtggtaag agtaccgttg ccaatgcgtt tgctgatctc
    36481 ggaattaacg tcattgatgc cgatattatt gcgcgtcagg tggttgaacc aggtgcacct
    36541 gcgctacatg ccattgctga tcactttggc gaaaacatga ttgctgctga tggaacattg
    36601 cagcgccggg ccttgcgcga gcggatcttc gccaacccgg aagagaaaaa ctggcttaac
    36661 gacctgctgc atccgctgat tcagcaagag acgcaacacc agatccagca agcaacttcc
    36721 ccctatgtac tgtgggttgt gccattgctg gtagaaaact cactgtataa aaaagcgaat
    36781 cgagtgctgg tggtggatgt cagcccagaa acgcaactta agcgcaccat gcagcgcgat
    36841 gatgtaactc gcgaacatgt cgaacaaatc cttgctgctc aggcaacgcg cgaagcccgc
    36901 cttgccgtgg cagatgacgt cattgataat aacggcgcac cggatgctat cgcatcggat
    36961 gttgcccgcc tgcacgcaca ctatttgcag cttgcgtcgc agtttgtctc acaggaaaaa
    37021 ccgtaatgca gacccaggtc ctttttgaac atccactaaa tgaaaaaatg cgtacatggc
    37081 tgcgcattga gtttttgatt cagcaactca ccgttaattt acccatcgtt gaccacgctg
    37141 gcgcgctgca tttcttccgt aatgtcagtg aattactgga tgttttcgag cgcggcgaag
    37201 tccgcactga gctgttgaaa gaacttaacc ggcagcaacg taaactccag acctggattg
    37261 gcgtgcctgg cgtggaccag agccgtattg aagcattaat tcagcagtta aaagcggcgg
    37321 tgagcgtatt aatttccgcg ccgcgtatcg ggcaatttct gcgtgaagat cgtttgattg
    37381 ctctggtgcg tcagcgactg agcatcccag gcggctgttg cagctttgat ttacctacat
    37441 tgcacatttg gctgcatcta ccccaggcgc agcgcgacag ccaggtagaa acctggattg
    37501 ccagcctgaa cccgctcacc caggcactta ccatggtgct ggatttaatt cgccagtcgg
    37561 cccccttccg taaacaaacc agcctgaatg gtttttatca ggataacggt ggcgatgccg
    37621 acttgctgcg cctgaatctg tcgctcgatt cacagcttta tccgcaaatt tccggacata
    37681 agagccgttt tgccattcgt tttatgccgc tggacactga aaacggacag gtaccggaac
    37741 gtctggattt cgaactggcc tgttgctaag gagtaaaaat gtcagaaact attacggtga
    37801 attgcccaac ctgcgggaaa acggtggtgt ggggtgaaat cagcccgttt cggccatttt
    37861 gctccaaacg ttgtcagctg atcgacctcg gagaatgggc tgctgaagaa aaacgaatcc
    37921 ccagcagtgg cgatctttcc gaaagcgatg actggagcga agagccaaag cagtgacatt
    37981 tgtctgatgc ctcacgtagg cctgataaga cgcggacagc gtcgcatcag gcatcttgtg
    38041 caaatgtcgg atgcggcgtg aacgccttat ccgacctaca gacgcttaag cttcgcaatt
    38101 accggttcat tggctggcgg aaaatcatcg gcattaagac cgaccagcga catccactca
    38161 ccgggttgcc cttctttacc ccacggcacc ccttcccaac tttcgaccag ccagaaccac
    38221 agagttatat gcctgtccgg gaattcatat tccagttttt caaatagcga aaaatgttgt
    38281 ggggtaatcc cgacctcttc ctgaagttca cgcaccaccg cctgttccgg cgtttcgccc
    38341 atttcaattt taccgccggg aaactccagt ttattcgcca tgtgcgcatc tgctgcgcga
    38401 cgtgttataa agatttcatt gttctcgttg cgaataatac ctaccgcaat ttgcagcttt
    38461 ttcattgtct taaacctata aaaaaggcgc agaatcctgc gccttttact tcaactgtta
    38521 gcttttattg caggcgacca tggcactgct tgtatttttt accagaaccg cacgggcaag
    38581 gatcgttacg tcctactttg cgttcaccgg tttgcgccgc cagtgcagct gcggctgcag
    38641 agtcgtcatc ctgatggcta agctgctgca tttgtgctaa acgctcggct tccatacgac
    38701 gctgttgttc cagctcctca acctcttcag gcatacgtac ctgaactttg ctcagcgtac
    38761 tgataacttc atatttcaac gactccagca tcgctgcaaa catggagaac gattcacgtt
    38821 tgtattcctg cttcggatct ttctgtgcat agccacgcag gtggataccc tgacgcagat
    38881 agtccatcgc tgccaggtgc tctttccaca gggagtcaag cgtttgcagc atgacgcctt
    38941 tctcgaagtg acgcatcatc tcagcaccaa ccacttcttc tttacgctga tacacttcga
    39001 tggactgcgc cagaatgcgc tcacgcagcg tctcttcatg cagttctggt tctttatcca
    39061 gccactcggt aattggtaaa tcgaggtcga aatcgttctt cagacgttcc tgcagccccg
    39121 gaatatccca catttcttcc agcgactgtg gcggaatgta ggcatcaatg gtggctttga
    39181 acacatcttc acgaatgctg ttgatggttt cgctcacatc gctgacatcc agcagttcgt
    39241 tacgctggga gtaaatggcg cgacgctgat cgttagccac gtcatcatat tccagcagtt
    39301 gcttacgaat gtcgaagtta cgactttcaa ctttacgctg ggcgttggca atcgctttag
    39361 tcacccacgg gtgttcaatg gcttcgcctg gcttcatacc cagtttacgc atcatgccgg
    39421 atactcggtc ggaagcaaaa atacgcatca gcgcatcttc catcgacagg tagaaacggg
    39481 aagaaccagc atccccctga cgaccagaac gaccgcgcaa ctggttatcg atacgacggg
    39541 attcgtgacg ctcggtaccg atgatatgca ggccacctgc ttccagtacc gcatcgtgac
    39601 gtacctgcca gtcggcttta attttttcaa tttgctctgc ggtcggattt tccagcgcgg
    39661 caacttctgc ctgccagcta cctccgagca caatatctgt accacgaccc gccatgttgg
    39721 tcgcgatagt caccgcagcc ggataacctg cctgagcaac aatcgccgct tcgttggcgt
    39781 ggaatttggc gttcaggaca ttgtgcttaa taccggcttt ggtcagttcg tttgacacca
    39841 gctccgattt ttcgatggag atagtaccca ccagcaccgg ctggcctttc gcagtacgtt
    39901 ctttgatatc ttcaatgatc gcctgaattt tttccgcttc agtcatgtag accaggtccg
    39961 gcagatcttt acgaatcatt ggacggttgg tcggaacaac gacggtatcc agtttgtaga
    40021 tggagctaaa ttcgaaagct tcggtatcag cagtaccggt catccccgcc agtttttcat
    40081 acagacggaa gtagttctgg aaggtgatcg aagccagcgt ttggttttcg ttctggatct
    40141 gcacaccttc tttcgcttcc acagcctggt gcagaccatc ggaccagcgt cggccctgca
    40201 tggtacgacc ggtgtgttcg tcaacgatga taacttcacc atctttaacg atgtagtcga
    40261 cgtcacgggt aaacagcgca tgagcgcgca gcgccgccgt tacgtggtgc atcagcatga
    40321 tgttggccgg agagtacaga gactcccctt catccatgat gccctctttc accagcagtt
    40381 cttcaatcag caccagacca cgttcggtca ggttcacctg gcgagatttt tcgtccaccg
    40441 agaagtggcc ttcgccctgg aaggtttcgg agtcttcttt ttcctgacgg atcaggtgcg
    40501 gaataatttt attcacgcgt ttatacattt ccgagctgtc ttctgccggg ccggaaatga
    40561 tcagcggtgt acgcgcttca tcgatcagga tggagtccac ttcgtccacc agcgcatagt
    40621 gcagtttacg ctgtacacgt tcttcagggc tgaacgccat gttgtcgcgc aggtagtcaa
    40681 agccgtattc gttgttcgta ccgtaagtga tgtcagctgc gtaagcttcg cgctttgccg
    40741 gtgctggcat gcccggcagg ttgataccaa cagtcaggcc aaggaattca aacagcggac
    40801 ggttgttttc ggcgtcacgt tgcgccaggt agtcgttgac ggtaactacg tgcacgcctt
    40861 taccggttag tgcgttcagg taagcaggca gcgttgcggt cagggtttta ccttcaccgg
    40921 tacgcatttc ggcgatgcag cgttcgttaa gaaccatacc gccgagtaac tgaacgtcga
    40981 agtgacgcat accaaagacg cgcttacttg cctcacgcac cacggcgaaa gcttccggga
    41041 tcagattttc cagcacttcg cctttttcca gacgcgcacg aaactctgcg gttttccctt
    41101 tcagttcttc atcggagagt ttttccatct ccggttccat ggcattgatg atgttgacca
    41161 ctttgcgcat ccggcgcagg gtgcgatcgt tacgactacc gaaaacttta gttaacaatt
    41221 tgattagcat aataaaatct caaacgcccc gcgttacgga gttaataaaa tgaagtaaag
    41281 gtttattgtt gttaggtgag gcgttgaggg ccagcacgga tgccttgcgc ctggcttatc
    41341 cagacgggcg tgctgaattt tgcttgtggg gtaaaatgcg cataatcaat gcgataaccc
    41401 ttttcagacg gcgtgccttc ctgggtcagc agcgcgctga gcgtatccag taatgcaaga
    41461 tgttgcgcct gaagaggcaa agattcttca gcaacgggca gtgtttgcgg tgccattgcg
    41521 aaagaaagat gacggattac cgtacgaata gcatgttgat gccagtaatc aacggaatag
    41581 ttcgaattcg ggcggcgcgt gttcgcttcc agcaaggcca attgaccaaa gttaactttg
    41641 gctgaaggct cgtggttgcg ggttgtcgct tttgcgggcg cgtttggttc ggcggcgttg
    41701 ctgagcgcag gcaaacctaa actcgccgcg accatcccta ataagagatg cggccagaag
    41761 tagcgtttac caaactgtcg ccagcgcgtc agtattccac tcacgttatt gccatcccga
    41821 tcgaattcaa acgtatttag cgcacaaatc ttatcattaa agcctgaagt ccacagcacg
    41881 aatgaagata agttgtgcgt aaaagtgctt aagaaaacag cgttcgcacc gttttttata
    41941 gctcttataa aattcaaaaa aaaacgactg gttcacctgg ccggagagag tgccagattt
    42001 gccagtcgaa ttttatacga cagtataaat gtcgttatgc cagtacagct gaaggcgctt
    42061 tgaaggccaa cggcagttct gcgtcgtcct ggaaggtcac atattcccag gcttcctgtt
    42121 tcgccaggac agcctgcagc agtttgttat tcagtgcatg accggattta taagcggtaa
    42181 atgcaccaat aatattgtga ccacacatga acaagtcacc gatcgcatcg agcattttgt
    42241 gacgcacaaa ttcgtcttca aaacgcaggc cgtcttcgtt cagtacgcga taatcgtcaa
    42301 caacgatggc acaatcgaag ctgccgccca ggcacaaacc acgggactgc agatattcga
    42361 tatcacgcat gaaaccgaac gtacgcgcac ggctgatctg gcgcataaac gcatcagcgg
    42421 agaagttcat cgcatagcgc tggttgctgg aatcaatagc cggatggtta aaatcgatgg
    42481 tgaaatccag cgaaaaacca ttgtacggct taaattcagc ccacttatcg ccatcttcga
    42541 cacgaacagt ctctttgatg cgaacaaatt ttttggcgca gttcaactcg tcgataccgg
    42601 cgtcaagcag caggtataca aacggagcgg cgctgccgtc catgatcggg atttccggcg
    42661 cgttaacttc gataacaatg ttatcgatgc ccaggcccgc gagagcagca ttgaggtgct
    42721 ctacggttga aatccgtaca tcatgctcgt tgaccagaca cgtacagagc atggtatcac
    42781 gcacagattt ggcatcggcc gggaaatcta ccggtggatt caagtcggtg cgacgataga
    42841 tgaccccggt gttggccggc gcagggcgta acgtcagggt gactttcttg ccggtatgta
    42901 aaccgacacc cgtcgcctga acgatacgtt taagtgtcct ttgtttgatc atcgtattat
    42961 ctcgccaaat tacctatcca accgaagtgt actatacatt cggcgggcca gtttagcaca
    43021 aagagcctcg aaacccaaat tccagtcaat tcttaatcag cttgcttacg taggaatgct
    43081 gggatatcca gataatccgg ctctttcgca gtttgcggcg cattgtcatt cactacttta
    43141 gcaaccggct tctgctcctg ggtcagcgga gccatcccat gctgctggta gcgatccatc
    43201 actggctgct gaacctgctt attggtcacc agagtgattt caggacgttt gtccatgccg
    43261 atacctgtcg caacaacggt tacgcgcagc tcgtcattca tatccgggtc aagagaagta
    43321 ccgataacca cagtcgcgtt gtcggaagca aatgcacgga tggtgttacc taccgtttcg
    43381 aactcatcca gacgcaggtc gaagcccgcc gtgatgttaa ccagcacgcc gcgcgcgcca
    43441 gacaggtcga tatcttccag cagcggagaa gagatagcca tttcagcagc ttcttccgca
    43501 cggtcttcac cgctcgccac gccagaaccc atcattgcgt agcccatttc agacattacg
    43561 gtgcgtacgt ctgcaaagtc cacgttcatc aaacccggac gagtaatcag ttcggcgata
    43621 ccttgcacag cgcctttcag tacatcgttc gctgcgccaa acgcatccag cagggagata
    43681 ccgcggccca gaactttcag cagtttgtcg ttcgggatag tgatcagaga gtccacatgc
    43741 ttggacagtt cagtgatccc ctgctcggcg aatgccatac gcttcttgcc ttcaaagttg
    43801 aaaggcttag tgacgacagc aacggtcagg atacccaaat cttttgccac ttcagcgacg
    43861 actggtgctg cacctgtacc ggtaccacca cccatacccg cagcaataaa gaccatgtct
    43921 gcaccttcca gcgccgcacg caatgcatcg cgatcctcat cagccgcatt gcggccaact
    43981 tctggattag cgccagcgcc cagtcctttg gtgataccgc taccgatttg aatcgtctgt
    44041 ccaaccgctg ttttacgcag cgcttgtgca tcggtattta ccgcgaagaa ttcaacacct
    44101 tcaatacgct cgcgcaccat gtgttcaaca gcattaccgc cgccgccgcc gacgccgatg
    44161 actttaatca ccgcgtcatt ggtaagttcc attggttcaa acatagtttc tctccgattt
    44221 gtgcctgtcg cctgagtccg taatcatcgt cggcctcata aaaattaaaa ctcttttcgc
    44281 agccaactat tgagtcgctt gatccacgag ccaactgatg ctgtaacacg tttttctact
    44341 tcagcttcac cgttaagatg tgactctttc ccatagtgaa gcaatcccac cgccgtcgaa
    44401 taatacggct cctgagcata atccgttaaa ccggtaatgt tcagcggcgc gccgatacgc
    44461 acttgcgtat gaaacacgcg ctgagcacag gctgcaagac cttcgatctg cgctgcgcca
    44521 ccggttaata caatgcctgc cgccaggtga tgtttaaccc cttgttggcg aagcttttcc
    44581 tgcaactgca atatctcttc gttgaccagg ttgagcaact cggtatagcg cggctcgatc
    44641 acctctgcca gggtctgacg ttgcagactc cgcggcggac gaccgcctac gctcggcact
    44701 tccacgctct catcttttcc aacgatggaa cccagcgcac aaccgtggcg aactttaatc
    44761 gcttcggcgt cgcttggcgg cgtgccaaag gcgtaagcga tatcactggt cacgacattg
    44821 ccagcataag gaattacctt tgtgtggcgc aatgccccac cggtataaac ggcgatatcc
    44881 attgtaccac caccgatatc gacgacgcag acacccagtt cacgttcatc ttccgtcaat
    44941 accgaataac ttgatgccag tccggcaaat atcagttggt caactttcag cccacaacgt
    45001 tcaaccgctt tgacgatgtt tttcgccata tcgttgtgac atgtgatcag gtgcactttt
    45061 gcctgcatcc gcacacccga aagtcctacc ggattcttga tcccttcctg atagtcaatc
    45121 gcatactctt gcgggatcac atgcagcaca cgatgctcat cgcgcacacg caccgatttc
    45181 gcggtatgga cgacgttttc cacatcttct tgcgtcactt cttcttcaga aataggcacc
    45241 ataccaattt cattctggca gctgatgtgc ttaccagaaa gcgccagata taccgaagag
    45301 atctgacaat ctgccatcaa ttctgcctgg tcaatggcgc gttgtacgca cttgaccacg
    45361 gattcgaggt cgttcacccc gcctttatcc ataccacgtg acgggcagct gcccacgcca
    45421 atgatattga ccataccgtc gggcagaact tcccctacta aagcggcaac cttcgcggta
    45481 ccaatttcca gtcctactac cagttttctg tccgtcgcct tgatcattgt tgttctgcct
    45541 gtgcctgatt ttgttgctga gtagattcct ctggcggcaa gggcgcccag cctactgccg
    45601 ctccagagtc ataacgcaaa tcaacgtagc taatccgttt gccatcggtt tgcgcctgct
    45661 gctgtaaaac cggataaagt tctacaaagc gaaccaaacg tttcatcgta tcgccccggc
    45721 caagattgag cttaatatcg ttattcagcg tcaactgcca ggaacgccgc gcggtcatcg
    45781 ccgcttcctt cagagtaaat ctgtcctttg ccagcatctg ccccatttcg cgatagccct
    45841 gcaacacttc attagcgctg ccttccgggc catacagcat tggaagcacc tgcttgctgg
    45901 tgcgatctgg cggcacgctg aaggtgtttc cttccgcgtc taccatgtgt tgatcattcc
    45961 accgcgcaat cggcacatat tcaaccagat gaatcttcaa ttcatcaggc cactgctttc
    46021 tgacgctcac ctgcttaatc cacggcaggc gttgttctat ttgcgtctgg atgatgttga
    46081 catcctgggt cataaaggta cccggctcac ccaatgccag gatcgactgc cggatatcgt
    46141 cattacgcgt gtaatggcgt tcaccggtca acaccagctt tgagagcggc aggcgttgcg
    46201 catcttccat ccagctcaac acgacccagc cgctcaccaa cactgtcgtt aaaacggtca
    46261 gcaggaaaag gatccccgcc agacgcgttc cattattgcg gcgagaagaa acctcttctt
    46321 cgctgtttcg cgtgttcaga gcagcctgcg acatattagt ccgccagttc cagaattcgt
    46381 actaccaact gcgagaagct catacctgcc tgacgtgccg ccatcggcac caggctgtgg
    46441 ctggtcatac ccggtgaggt attggcttcc agcagataaa actgtccatc actgtccagc
    46501 ataacgtcaa tacgtcccca tcctttgcaa cctaacgtcg tccatgcttt cagcactaat
    46561 gcctgcaaat tggcctcttg tgacgcttcc agacctgcgg ggcagaaata ctgtgtctca
    46621 tcagagagat acttcgcctc ataatcatag aaggttccgg acggttgaat acgtattgac
    46681 ggtaaaattt cttcaccgag tatcgcaacc gtgaactccg gcccacttag ccatttttca
    46741 atcaatactt cttcatcgtg ctgaaatgcc aatcttaatg catcttgtag agcattttct
    46801 gctactactt ttgacattcc cacactggaa ccttcgcggc tcggcttaac gataaccggc
    46861 aaacccagag cagaaatttc tgctaactgc ttatcgctca ggcctttttc aaactctgcg
    46921 cgggttaacg ctacccacgg cgcgaccggt aaaccggcac cttgccacag aagtttgctg
    46981 cgtagtttat ctattgaaag cgcagatgcc atcactccgc tgccggtata aggcaagccc
    47041 atcagctcga gcatgccctg cagcgtacca tcttcaccgc cgcgaccgtg tagcgcgata
    47101 aacactttct gaaaacccat cgacttcagt tgcgtcacgt cgacttcttt cgggtcgaca
    47161 ggatacgcat caataccgcc ttcacgcagc ccggctaaca ccgctgcgcc agaattcaga
    47221 gaaacttccc gctcagcgga agtcccgccc aacaggaccg cgattttatc agtcatgttg
    47281 ttcttcctcc ggagtttgcg gcttcagttt gatttcagct aaagaacggg caatttttcc
    47341 gatattacca gccccctgaa cgagaatcag gtcgttaccg gttaataccg gtgccagcat
    47401 ctcggctacc tgcgcagggt ccggcaccag aatgggatca attttcccac gtccacgaat
    47461 tgtgcgacac agcgaacggc tgtccgctcc cggaattggc gcttcgccag ccggatacac
    47521 ttccagcatc aacagggtat caacctgcgt cagcacattg gcgaaatcat catacaggtc
    47581 gcgcgtacgg gtaaaacggt gcggctgaaa cagcattacc aggtttttat ccggccagcc
    47641 tgcgcgcgcc gctttaatgg tggcgtcaac ttccgtcggg tggtggccgt agtcatcgac
    47701 cagcattgcc gtaccgcttt taccattcac tggctccagc gggaattcac cgaggaaatc
    47761 aaaacggcga ccagtcccct ggaagctctc cagcgcacgc aaaatagcct cgtcgtcaat
    47821 tccctcttcc gtagcaaccg caaccgcagc tgcggcgttc agcgcgttat gacggcctgg
    47881 cgcattcagg gtgacgcaca tcggctcttt gtcctgacgc agcagcgtaa agtgcccctg
    47941 cgggccaatc tgctgataat cttctacacg cacgtcggca tcttcgctga agccgtaagt
    48001 tgtggtctga cgtcccacac gcggtaacaa ttcgcggatc accggatcat caacacacat
    48061 caccgcacga ccgtaaaaag gcaggttgtg cagaaaatta ataaaggtct gttttaaatt
    48121 ctcaaagtcg ccctggtagg tatccatgtg gtcggcttcg atattggtga caatcgccac
    48181 catcggttgc agatgcagga acgatgcatc actctcatct gcttcggcaa tcaggtaccg
    48241 accatgcccc aaacgcgcat gaacccccgc cgcttttacc agcccgccgt taacgaaggt
    48301 tgggtcgagc cccgcttctg cgtagatgct ggaaaccatc gcggtagttg tcgttttgcc
    48361 gtgcgttcct gcaatggcga tgccatgacg aaaacgcatt aactcagcca gcatttcggc
    48421 acgacggatc accggaatac gcgcttcatg agctgcgaca atttccgggt tgtcggcaga
    48481 aatggcgctg gaaacaacga ccacgctggc atcacgtacg ttttccgggc gatggttgaa
    48541 ataaatcgtc gcacccagat tcagtaactg ctgcgtgacc ggatttggcg ctaaatcgga
    48601 accactgatc tgataacctt cattggccag aacttcggca ataccgccca taccggcacc
    48661 accaatgccg acaaaatgta tgtgccgaac gcgacgcatt tcgggcacga tggaacgcag
    48721 ttttgccaat tgttgtgtat tcattcttta cgccattaac ttcttaaatt catacgatgc
    48781 aaaaggcatc gctacaatta cgcccgggca gcccggctca cttcatttgc cactcgctcg
    48841 gtggcatccg gaatggatgc agcgcgggcg cgttctgcca tggttaataa ggtttctcgc
    48901 gaccacccgg ccagggtgtt ggcgacagca tccacgctaa gctgtggctg ctcgataatt
    48961 ttggctgcgc ccgctttttc cagcggtagc gcattccagt attgctggcg gtctttatgt
    49021 tgaaacggca caaacaacgc cggtagtcct gccgcggcga tttcactcac agttaaagca
    49081 ccggagcggc aaacgacgac atccgcccac gcatacgccg ccgccatatc atcaataaat
    49141 tcagtcactt tatgctgcgg ttgccccgct tcggcatacg cctgttcaac ggattgttgc
    49201 gaacctttgc cgctctgatg ccagatagtg actgaatcac ccagtttcgc agcaacctgc
    49261 ggcattgtct ggttaagaat gcgtgcgccc tgggaaccac ccactaccaa cacacgaacc
    49321 ggaccttcac gtccagccaa acgttgctgc ggcaacggca gcgccagcac atcggtacgc
    49381 accgggttac ccaccacttc cgcattaggg aaagcgcctg gaaacgcctg catcactttg
    49441 gtagcaatct tcgccagcca tttattggtt aagcccgcaa taccgttttg ttcatgaagt
    49501 acaaccggaa tgcctaacga ccacgcggcc agaccacctg gacctgatac atagccgccc
    49561 ataccgagta ccacgtcagg tttgtacgct ttcataatcg cccgcgcctg acgccaggcg
    49621 ttgaagatac gcagcggcgc agctatcagt gcttttatac cttttccacg caaaccagag
    49681 atacgaatga aatcaatttc gatgccatgt tttggcacta agtccgcttc catacggtcg
    49741 gcagtcccca gccagcgaac ttgccaaccc tgagccatta gatagtgcgc aaccgccagt
    49801 cccgggaata catgtccacc ggttccgcct gccatcacca ttaatcgctt tccttgacca
    49861 ctcatcgtga acctcgtaca aacgcctgcg ctttctccag acgcgtttca taatcaatac
    49921 gcaacagcat catgatggct gtcgacataa tcagtaagct cgaaccaccg taactgatta
    49981 gcggcaatgt cagacctttg gtcggtaaca tccccgccgc cgcgcctacg ttaaccagcg
    50041 cctggaagct aaaccagatg ccaatagaac aggcgagaaa accggaaaaa cggtggtcaa
    50101 tttctaatgc tttgcggcca atcgacatcg cacgaaaagc gacgaagaat accattaaaa
    50161 gtgctagtac cacaccgaca taacctaatt cttcgccgat aatggcgaaa ataaagtcag
    50221 tgtgcgcttc cggcagatac tccagttttt gtaccgaatt acctaaacct tgcccccaaa
    50281 gttcgccgcg accaaacgcc atcagcgatt gcgttaactg atagccgctg ccaaagggat
    50341 cttcccacgg gttccagaat gcggtaacac ggcggatacg gtacggttcg gcgaggatca
    50401 gcaacacaac cgctgaaatg cccataccga taatggcaat gaactgccac aatttcgctc
    50461 ccgccaggaa caacatcgcc agcgtagtca caaacaacac caccaccgta ccaaggtctg
    50521 gctgtgccag cagtaacact gccaacacca gaatcacgcc catcggtttc aggaagccgc
    50581 gcaggttatt acgtacttcg tcgcctttac gcaccagata gttggcgata tagcaaaaca
    50641 gcgacagttt tgtcagctcc gcaggctgga tacgcagcaa accgagatcg atccaacgcg
    50701 atgccccttt aacagcgcta cccactacca gcacgatcat cagcaggatg atagatccga
    50761 gcagcatcgt ggcactgtag cgttgccaga actccatcgg cagacgcagc gtaatgatcg
    50821 ccagaataaa cgccaaaatc agatagacac catcacgctt cgcgaagaag aacggatcgt
    50881 tggttaagcg ttgccctatg ggcattgacg ccgaggtcac catgataaag ccaatcgccg
    50941 cgaggccgaa ggtcagccac agtaaggtgc gatcgtacat gatcaggctg tcggtatctt
    51001 tttcccgcga gcccatcacc cagcccttta gcgccgtgga gatccagacc aggatactga
    51061 atcctggcag gcgcggcatt ttcaggcgag ggagagataa acgcatcaac ctaactcctt
    51121 cgccagacgg gcaaactcat tgcctcgttg ttcaaagttc ttgaactgat caaggctggc
    51181 acaggccggg gagagcagaa ccatatcgcc cggctgaaca cgcggagcca gcaagcgcat
    51241 agcctgttcc atagtttcgg tttgttctgc cacttccggg cgtagcgccg ccagctgcgc
    51301 gccgtcacga ccgaaacaat acagacgtac gttatcgcca ttcaggtaac gcaccagtgg
    51361 gctaaagtcc gccgatttac catcgccgcc cagcaacaaa tgcagcgtgc cgtctacgtg
    51421 caggccattc agcgccgctt ccgtactgcc gacgttggtc gcttttgaat cgttaatcca
    51481 gcgcacaccg ttatgctcca gcaccacttc aaagcgatgc ggcagaccgg tgaaggtggt
    51541 taacgctttc aggctgctgg cgcgcggtaa ccctgcggca tctgccagcg ccagcgccac
    51601 cagcgcattg gtgtagttat gctgcccgga aagtttcatc tctttcacat tcagcacttt
    51661 ctcgccttta acccgcagcc aggtttcgcc ctgctgatga ttcaggtgat agtcacccat
    51721 gttgacgcca aagctgacgc agcgttcatc cgcaccgcga atcggcattg ttaaggcatc
    51781 atcagcatta accacgcaaa ctttcgcgtt ttcgtaaatg cgcagttttg ctgcacgata
    51841 ctgttgtaaa ccaaacgggt agcgatccat atgatcttca gtcacgttca gaatggtcgc
    51901 tgctaccgcc tgtaagctgg aggtggtttc cagctggaag ctcgacagtt ccagcacgta
    51961 cagttcacac tcatcatcca gtagcatcaa cgcaggcagg ccaatattgc cacccacacc
    52021 aacgttaacc cccgccgctt tcgccatttc acccactagc gtggtgaccg tgcttttgcc
    52081 gttagaaccg gtaatcgcca caatcggtgc ttgtgcttcg cgacaaaaca gctcgatatc
    52141 gccaacgatt tcgattccgg catcagcggc agcgcttaag gatggatgcg ccagtgcaat
    52201 accgggactg gcgacaatca gatctgccgc catcagccat tcatcattca gactgcccgt
    52261 gtggcgttct acggcttcgg gtaatttatc caggccaggc ggtgtcatac gcgtatccat
    52321 aacgcgcggc gtcacaccgc gagcgaggaa aaagtccacg caggaaagcc cggtgaggcc
    52381 caggccgata atgacaacat ttttaccctg gtaatcagcc atgattaacg taccttcagc
    52441 gttgccagac caatcaggac cagcatcagc gaaataatcc agaaacgcac aatgacgcgc
    52501 ggttccggcc agcctttcag ttcatagtgg tgatgaatcg gtgccatgcg gaaaatacgt
    52561 tgtccgcgca gtttaaagga gccgacctgc aggatgacag aaagcgtttc taccacgaac
    52621 acgcccccca taatcaccag caggaattcc tgacgtagca gtacggcgat aatgcctaac
    52681 gcaccaccta acgccagcga accgacatcg cccataaaga cctgcgccgg ataggtgtta
    52741 aaccacagga agcccagtcc tgccccgact atcgcggtac agacaataac cagttccccg
    52801 gcgtgtcgca gatacggtat gtgtaaatag ctggcaaagt tcatgttgcc ggtcgcccac
    52861 gccaccagcg caaaaccacc ggcgacaaat acggtcggca taattgccag accgtcaaga
    52921 ccatcggtca ggtttaccgc gttgccagta cccacaatga cgaagtaagc cagcagaatg
    52981 tagaacagcc ccagttgcgg catcacatct ttaaagaatg ggaccaccag ctgcgttgcg
    53041 ggcgtgtctt tgccggcaag gtacaaagcg aaagcaacac ccagcgcaat gaccgacatc
    53101 cagaaatatt tccaacgagc gatcaaccct ttggtgtctt tacgcaccac tttgcgataa
    53161 tcatcaacaa agccaataac accgtaacct accagcacca ccaacacgca ccagacgtac
    53221 ggattggacg ggtaagccca cagcagcacg gagatcacaa tcgctgtcag gatcataatc
    53281 ccgcccatgg tcggcgtacc gcgcttgctg aagtgtgatt caggaccgtc gttacgcact
    53341 acctgaccaa aggaaagttt ttgcaaatga gcaatcatgc gcgggcccat ccacaatgag
    53401 atgaacagcg cggtcagcag gctgacgatg gcgcgaaacg tcagatagga aaagacgtta
    53461 aagccggaat aatatttgac caaatgttcg gccagccaaa ctaacatgtc ccattctcct
    53521 gtaaagcgcg tactacctct tccatggcag cactacgtga acccttaact aaaatcgtaa
    53581 ttacctgttg ctcagcaatc agtgatttaa gacgcgtaat taacgcagtt ttatcagcaa
    53641 aatgttcgcc aacgccgctg gcggtgctga tagcatggct ttgtttaccc atgcttaaca
    53701 cgcggtcaat accagcagct tttgccgcct cacccacctg tacatggcag gcttcgcttt
    53761 cagcgcccag ttccgccata tcgcccacca ccagcacgcg gtagcccggc atttcagcca
    53821 gtacctgaac agctgcggtc attgaaccga cattggcgtt gtaggagtcg tcgagcagca
    53881 actggttttc tgccagttgg atggggaaca gacggccagg aacagctttc agatttgcca
    53941 gccccgcttt gatagcatca agcgttgcgc ccacggacat ggagagcgca gcggctgcca
    54001 gcgcattcgc aatattgtga cgccccggca acggcagcag aacatcgacg ctaccggttg
    54061 gggtttgtag ggtaaattcc ataccgtgcg aggtcacatg gatattggtg gcggtgaaat
    54121 cgctgttggc ggcattgggt gagaaacgcc acactttgcg tgagccaatt acgctctgcc
    54181 agttcagcca gtcgttgttg tcggcgttca taatggcgat accgttttcc ggcaggccgc
    54241 taaagatttc acctttcgct ttcgcgacac ccgcaagcga gccaaaacct tccagatgcg
    54301 ccgctgccag gttgttgacc agcgccgctt ccgggcgagt cagactcaca gtccaggcaa
    54361 tctcgccctg atggttcgcg ccaagttcaa taactgcgta atcgtattcc ggcgttaagc
    54421 gcaacagcgt catcggtaca ccgatgtcgt tgttgagatt gcctgccgta taaagcgtgt
    54481 tgccgcactg gcttaaaatc gccgccgtca tctctttaac ggaggttttg ccggaggacc
    54541 ccgtcagagc aaccacgagc gccggaactt gctggcgaac ccatgcagcc agttcaccaa
    54601 acgccagacg cgtatccttg acgattaact gcggcaggtc gatgtccagc ggacggctaa
    54661 ccagtagtgc gcctgcgccg ccagctttcg cctggtcggc aaaatcgtgg gcatcaaaac
    54721 gttcaccttt cagggcaaca aacaggcagc ccggcgtcag ttttcgggta tcagtggtta
    54781 cagcatcaag ggtgatatct gcaccttgca gttcaccgtt gagaatatcg gtaagttggc
    54841 taagggttac gctaatcatg caatcccccc cagcagacgc gccaccgtga cgcgatcgga
    54901 gtagtccaga cgctgattgc caacaatctg gtaatcctca tggcctttgc ccgcgaccag
    54961 taccacatca ttctctttag cctgcataac ggcgcaagtc accgcttcag cacggccttc
    55021 catcactttg gcatgtccgg catctaacat tcccgccaga atatcgttga tgatggcacg
    55081 cggttcttcg gtacgcgggt tatcgtccgt caccaccgcc acgtcagcaa actcttcggc
    55141 aattgcgccc atcagtggac gcttaccttt atcgcgatcg ccaccacagc caaagacgca
    55201 ccacagcttg cccgcacagt gcaggcgcgc cgcctgtaag gctttttcca gtgcatccgg
    55261 cgtatgcgcg taatccacca ccaccgtcgg tttgcctggc gcagtgaaca cttccatacg
    55321 tccgcaaacc ggttgcagac gcgcggcggt tttcagcaga tcagccagtg gatagcccag
    55381 cgccagcagt gttgccagcg cgagcaacag gttgctgacg ttaaaagcgc ccatcagatg
    55441 gctttcaatt tcgccatcgc cccagcttga actaaagcga atggtcgcgc cgctgtcgtg
    55501 atagttcact tcggtcgctt tcaaccagcg tccgtgacag ttcggattga tatgatcttc
    55561 cattgatacc gcaaccgcgt ccggcagttt tgccagccag cggcggccca cttcatcgtc
    55621 ggcgttaata atcgcctgac cgcaatgatg ttcagaataa agcagccatt tcgcagcttc
    55681 gtagtgttcc atatcaccat gataatcaag gtgatcgcgg cttaagttgg taaagaccga
    55741 cgccggaaat ttcaatgccg ccacacggtg ctgtaccagc ccgtgggagg aaacttccat
    55801 tgcgcaaaaa gtcgcgccct gatccaccag ccccgccagc tcatgctgaa catcaactgc
    55861 cgaaccggta gtattttctg tcgggatcac tttccccagc agaccgttac caacggtgcc
    55921 cattaccgcg ctggtttcgc caagcagttg gctccactgc gccagaagct gggtcgtcgt
    55981 ggttttgccg ttggtgcccg ttacgcctac gagacgtaaa ttgtcagagg gttcatggta
    56041 aaagcggccc gccagtgcag ataaacgctc gttgagttgg ctgagataga tgaccggtac
    56101 gccgtgcatt tcacggattt caccgtcggt cgcctcatct ttcgcctctg caataatggc
    56161 agccacacct tgcgctatcg cctgcgggat atatcgacgc ccgtccgcct gatgacctac
    56221 tacagctaca aagagatcgc ccgccgcagc cacacggctg tcgagtgtca tctctcgcag
    56281 tgctcgcgaa ggtgcgtctg gcacccacgg agcaagaagg tcgcgcaaat tacgatctgc
    56341 cacctgtccc ctcgccttga ttaatcacaa attcattttt atcgcccgtt gtcagcgcat
    56401 ccggctcgat gttcatggta cgcaatacgc cgcccatgat ggcaccaaag accggcgcgg
    56461 aaacggcgcc gccgtagtat ttacccgcct gcggatcgtt gataacaaca accagcgcga
    56521 agcgcggctg actcgcaggc gcaacgcctg cggtataagc aatatattta ttgatgtagc
    56581 gaccgtccgg cccgaccttt ttcgcggtac cggttttaat ggcgatacga tagcctttaa
    56641 tcgccgcctt cacgccgccg ccgcctggta gcgccacgct ttccatcata tgcaccacgg
    56701 tgcgaacaat ggattccggg aagacacgtt caccgggaac cggggggtca actttggtaa
    56761 tcgacagtgg gcgataaatg ccgtagctgc cgatagttgc gtagactcgc gctaactgta
    56821 acggtgttac cattagcccg tagccgaaag agaaggtggc cctctctatg tcagaccacc
    56881 gttgtttttg aggatataag ccactgcgtt ctccgaccaa ccccaaattg gtcgcttttc
    56941 ccagtccaaa acgtgagtaa gtatctacta acgctgagga cggcatcgtt aacgccagct
    57001 tggaaacacc gacgttactc gacttctgta ataccccggt cagggttaat tcgctgtagc
    57061 gtgccacgtc tttgatttcg tggccgttaa ttcgataagg aatggtattg agtaccgagt
    57121 tttcccgcac cacgccacgt tgcaacgcgg tcatcaccac catcggttta accgttgagc
    57181 ccggttcaaa cacgtcggtg atggtacggt tacgcatcgc ctctttcggc gtgccgctca
    57241 gattgttagg gttgtatgac gggctgttag ccatcgccag cacttcacct gtgttgacat
    57301 ccaccagcac ggcgctacca gattcagcct tgttaaaggc caccgcgttg ttcagttcgc
    57361 gataaaccag cgcctgcagg cgttcatcaa tactcagcgc caggttgtgc gctgcctggc
    57421 tgtcagtaga agaaatatct tcaattacgc gaccatagcg gtctttacgc acaatgcgct
    57481 cacccggctg cccggtaagc catttatcga aactcttctc aacgccctca atcccttgac
    57541 tatcgacgtt agtaaagccg atgaggtgag cagtcacttc gccggacgga tagtaacggc
    57601 gagactcttc acgcagatga atccccggca gtttcagttt tttgatgtag tccgccatgt
    57661 cagagtttac ctgacgcgcc agataaataa agcgcccttt cgggttggcg ttaatgcggg
    57721 cagaaagctg atccagcgga atattgagcg cgttagccag cgccttccag cggtcaccga
    57781 cgctgatacc gccagcgtca tgcacttctt tcgggtcagc ccaaatcgct tttaccggca
    57841 cgctcaccgc taacgggcga ccagaacggt cagtaatcat gccgcgggag gtggaaactt
    57901 gctgaacgcg aagagaacgc atgtcgccct ctttcaccag catatccggg gagataactt
    57961 gtaaccacgc tacgcgtccg agcagaaaag ccagcgccag gagaatacag ccgcataaca
    58021 acgcaaaacg ccaactgata aagttggcat gttcttcctg acgttttggt ttctgcgttt
    58081 tcgccgctgc tttcatgcgt cgcgtttatc cttatttttg cactacgata ttttcttgtg
    58141 acggatcaac atgctgcatt tgcagctttt ccgtggcgat cctttccacc cggctatggt
    58201 cgccgagcgc attctcttca aggatcaggt tgcgccattc aatgtctaaa gcatctcgct
    58261 ccagcaccag ttgttcgcgc tgagcggtca gtaaacgggt atggtgcgcc gtggttacca
    58321 cggtcaccgc cgtcaaaata atgcaaatga acaggcagag tggcagcttc ccaaatcgca
    58381 aaagatcgtc accgataaca ccaggcaatg catggcgctc gtggcttccc atcgatcctt
    58441 taactttgct tagagcttct gtcactctgc tgatcatgcg ttcgtcctct ctgcaatacg
    58501 cagaactgaa ctacgggcac gagggttctc agccacctct tcttcgcccg gcattaactt
    58561 gcctagtgct cgcagctgac ggccacccag ttttttgagc tgctcttcag tcatcggtaa
    58621 ccctgccgga acttgcggac cgcggctgtt ttcacgcata aaacgtttca caatacggtc
    58681 ttccagcgag tggaagctga tgatcgaaag ccgcccaccc ggggccagca cgttgagcga
    58741 gctttttagc gcctgctcta tctcctccag ttcactgttt acccaaatgc gcaccgcctg
    58801 gaaggtacgg gtcgcgggat gtttaaactt atctttcacc ggcgttgcag cagccacgac
    58861 ttccgccagt tctttggtgc gggtcatcgg ctgttcgcgg ttacgctcga caatggcgcg
    58921 ggcaatgcgt ttggcaaaat gctcttcacc ataggttttc aatacccagg cgatatcggc
    58981 ttcttctgcg gtttgtagcc attcagcggc tgactgccca cgggttgggt ccatacgcat
    59041 gtccagcgga ccatcgcgca taaaggaaaa gccgcgttca gcatcatcaa gttgcggtga
    59101 agagacgcca agatcgagga gaatgccgtc gatcttgcca ataagatcgc gctcggcaac
    59161 gtattcgccc agcgcggaga aaggtccgtg gatgatggag aagcgcggat catcaatagt
    59221 cttcgcaacg gcgatagcct gcgggtcgcg atcgatcgcc agcaaacgcc cctcttcgcc
    59281 aagctgcgag aggatcagac gtgagtgacc accgcgacca aaagtcccat caatgtagat
    59341 gccatcagga cggatattga ggccattaac ggcttcatcc agcagcaccg tagtatgttt
    59401 atagttttcc atcattttat agagacaagt cctgcagtcg ctccgataag tctccggtag
    59461 ccaactgctc tgcgtcgata tcttccttga cctgttgatg ccaggttgtt tcatcccaca
    59521 gctcaaactt gttgaactgt ccaaccagca tcacttcttt tgtcagcccg gcatgttgcc
    59581 gcagtactgg cgcgattaac aatcgacctg cgccatccat ctgacattcg ctggcatgac
    59641 ctaacagtag gcgctgcaca cggcgctcaa ccgggttcat gctcaacaga cgcgataatt
    59701 tttgctcgat aatttcccat tcaggcaggg ggtaaagcag caggcacggg tgatgaatgt
    59761 caatggtgca aaccatttga ccggcagcgt tctcaagcag ctgttcccga taacgggtag
    59821 gcactgataa gcgccctttg ctgtcgagat tgactaacgt tgctccccgg aacatgccag
    59881 cctcacccct tattcccact ttgccccaca atttcccact gaaaggagtt tacggagcgg
    59941 aggaaaagct tgtcaagcca gtcacaaggc ataccgaggt aaaaaacccg atgtttacgg
    60001 ctaatatcag cgttgtttat tttacgtcca acgtgcgaag caaattaacg ttatgaatag
    60061 ctgtaagaaa aaatccctca tcgtcatcac tgttttaaaa tgactcacta ttttccacag
    60121 caaatataaa gtgtcagttt gcgacgcgag cggcatttta ggacatatct tccccggtta
    60181 acagtccttg ttacgtctgt gtggcgcttg atgccagcgg gtttgcgcaa aagcaaagaa
    60241 aagtgtttgt taaatcggca attcatcgcc gcatgtaaca aaataatgca aaaacgcgct
    60301 gtcgatatca cttaattatt gtgaatttca gctggtgagt taattaacgg gcgggtttaa
    60361 aaccatttat caataatagt caggaatagt cttatttact ttaaggatat tgat
//
LOCUS       DAJTSA010000013        59925 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000013 DAJTSA010000000
VERSION     DAJTSA010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.013"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            533..1822
                     /gene="sdaC"
                     /locus_tag="OO369_000860"
     CDS             533..1822
                     /gene="sdaC"
                     /locus_tag="OO369_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR5981233.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1880..3247
                     /gene="sdaB"
                     /locus_tag="OO369_000861"
     CDS             1880..3247
                     /gene="sdaB"
                     /locus_tag="OO369_000861"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR5981234.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3358..4113
                     /gene="xni"
                     /locus_tag="OO369_000862"
     CDS             3358..4113
                     /gene="xni"
                     /locus_tag="OO369_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR5981235.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="OO369_000863"
     CDS             complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="OO369_000863"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR5981236.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="OO369_000864"
     CDS             complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="OO369_000864"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR5981237.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6536..7852
                     /gene="fucP"
                     /locus_tag="OO369_000865"
     CDS             6536..7852
                     /gene="fucP"
                     /locus_tag="OO369_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR5981238.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7885..9660
                     /gene="fucI"
                     /locus_tag="OO369_000866"
     CDS             7885..9660
                     /gene="fucI"
                     /locus_tag="OO369_000866"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR5981239.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9769..11188
                     /gene="fucK"
                     /locus_tag="OO369_000867"
                     /pseudo
     CDS             9769..11188
                     /gene="fucK"
                     /locus_tag="OO369_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11190..11612
                     /gene="fucU"
                     /locus_tag="OO369_000868"
     CDS             11190..11612
                     /gene="fucU"
                     /locus_tag="OO369_000868"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR5981240.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11670..12401
                     /gene="fucR"
                     /locus_tag="OO369_000869"
     CDS             11670..12401
                     /gene="fucR"
                     /locus_tag="OO369_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR5981241.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="OO369_000870"
     CDS             complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="OO369_000870"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR5981242.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13538..13933)
                     /locus_tag="OO369_000871"
     CDS             complement(13538..13933)
                     /locus_tag="OO369_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR5981243.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="OO369_000872"
     CDS             complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="OO369_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR5981244.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15220..15447)
                     /locus_tag="OO369_000873"
     CDS             complement(15220..15447)
                     /locus_tag="OO369_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR5981245.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15639..16844
                     /gene="csdA"
                     /locus_tag="OO369_000874"
     CDS             15639..16844
                     /gene="csdA"
                     /locus_tag="OO369_000874"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR5981246.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16844..17287
                     /gene="csdE"
                     /locus_tag="OO369_000875"
     CDS             16844..17287
                     /gene="csdE"
                     /locus_tag="OO369_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR5981247.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="OO369_000876"
     CDS             complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="OO369_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR5981248.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18382..19479)
                     /gene="mltA"
                     /locus_tag="OO369_000877"
     CDS             complement(18382..19479)
                     /gene="mltA"
                     /locus_tag="OO369_000877"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR5981249.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFVEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19688..19764
                     /locus_tag="OO369_000878"
     tRNA            19688..19764
                     /locus_tag="OO369_000878"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19722..19724,aa:Met,seq:cat)
     gene            19798..19874
                     /locus_tag="OO369_000879"
     tRNA            19798..19874
                     /locus_tag="OO369_000879"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19832..19834,aa:Met,seq:cat)
     gene            complement(19949..21202)
                     /gene="amiC"
                     /locus_tag="OO369_000880"
     CDS             complement(19949..21202)
                     /gene="amiC"
                     /locus_tag="OO369_000880"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR5981250.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21434..22765
                     /gene="argA"
                     /locus_tag="OO369_000881"
     CDS             21434..22765
                     /gene="argA"
                     /locus_tag="OO369_000881"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR5981251.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22827..24653)
                     /gene="recD"
                     /locus_tag="OO369_000882"
     CDS             complement(22827..24653)
                     /gene="recD"
                     /locus_tag="OO369_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR5981252.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24653..28195)
                     /gene="recB"
                     /locus_tag="OO369_000883"
     CDS             complement(24653..28195)
                     /gene="recB"
                     /locus_tag="OO369_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR5981253.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28188..31076)
                     /gene="ptrA"
                     /locus_tag="OO369_000884"
     CDS             complement(28188..31076)
                     /gene="ptrA"
                     /locus_tag="OO369_000884"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR5981254.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31252..34620)
                     /gene="recC"
                     /locus_tag="OO369_000885"
     CDS             complement(31252..34620)
                     /gene="recC"
                     /locus_tag="OO369_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR5981255.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34633..34983)
                     /locus_tag="OO369_000886"
     CDS             complement(34633..34983)
                     /locus_tag="OO369_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR5981256.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            complement(34968..35375)
                     /locus_tag="OO369_000887"
     CDS             complement(34968..35375)
                     /locus_tag="OO369_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR5981257.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35372..35935)
                     /locus_tag="OO369_000888"
     CDS             complement(35372..35935)
                     /locus_tag="OO369_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR5981258.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35926..36396)
                     /locus_tag="OO369_000889"
                     /pseudo
     CDS             complement(35926..36396)
                     /locus_tag="OO369_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36580..37374)
                     /gene="thyA"
                     /locus_tag="OO369_000890"
     CDS             complement(36580..37374)
                     /gene="thyA"
                     /locus_tag="OO369_000890"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR5981259.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37381..38256)
                     /gene="lgt"
                     /locus_tag="OO369_000891"
     CDS             complement(37381..38256)
                     /gene="lgt"
                     /locus_tag="OO369_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR5981260.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38407..40653)
                     /gene="ptsP"
                     /locus_tag="OO369_000892"
     CDS             complement(38407..40653)
                     /gene="ptsP"
                     /locus_tag="OO369_000892"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5981261.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40666..41196)
                     /gene="rppH"
                     /locus_tag="OO369_000893"
     CDS             complement(40666..41196)
                     /gene="rppH"
                     /locus_tag="OO369_000893"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR5981262.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41881..42570
                     /gene="mutH"
                     /locus_tag="OO369_000894"
     CDS             41881..42570
                     /gene="mutH"
                     /locus_tag="OO369_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR5981263.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42639..43352
                     /locus_tag="OO369_000895"
     CDS             42639..43352
                     /locus_tag="OO369_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5981264.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43490..43708
                     /gene="ygdR"
                     /locus_tag="OO369_000896"
     CDS             43490..43708
                     /gene="ygdR"
                     /locus_tag="OO369_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR5981265.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43816..44856
                     /locus_tag="OO369_000897"
     CDS             43816..44856
                     /locus_tag="OO369_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR5981266.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44889..46079)
                     /gene="lplT"
                     /locus_tag="OO369_000898"
     CDS             complement(44889..46079)
                     /gene="lplT"
                     /locus_tag="OO369_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR5981267.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(46072..48231)
                     /gene="aas"
                     /locus_tag="OO369_000899"
     CDS             complement(46072..48231)
                     /gene="aas"
                     /locus_tag="OO369_000899"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR5981268.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDCSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48817..49848
                     /gene="galR"
                     /locus_tag="OO369_000900"
     CDS             48817..49848
                     /gene="galR"
                     /locus_tag="OO369_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR5981269.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49855..51117)
                     /gene="lysA"
                     /locus_tag="OO369_000901"
     CDS             complement(49855..51117)
                     /gene="lysA"
                     /locus_tag="OO369_000901"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR5981270.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51239..52174
                     /gene="lysR"
                     /locus_tag="OO369_000902"
     CDS             51239..52174
                     /gene="lysR"
                     /locus_tag="OO369_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR5981271.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52161..52853)
                     /gene="ygeA"
                     /locus_tag="OO369_000903"
     CDS             complement(52161..52853)
                     /gene="ygeA"
                     /locus_tag="OO369_000903"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5981272.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52982..54400)
                     /gene="araE"
                     /locus_tag="OO369_000904"
     CDS             complement(52982..54400)
                     /gene="araE"
                     /locus_tag="OO369_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR5981273.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54715..55476)
                     /gene="kduD"
                     /locus_tag="OO369_000905"
     CDS             complement(54715..55476)
                     /gene="kduD"
                     /locus_tag="OO369_000905"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR5981274.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(55506..56342)
                     /gene="kduI"
                     /locus_tag="OO369_000906"
     CDS             complement(55506..56342)
                     /gene="kduI"
                     /locus_tag="OO369_000906"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR5981275.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHCD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56629..57810)
                     /locus_tag="OO369_000907"
     CDS             complement(56629..57810)
                     /locus_tag="OO369_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR5981276.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            58065..59294
                     /locus_tag="OO369_000908"
                     /pseudo
     CDS             58065..59294
                     /locus_tag="OO369_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT        14798 a        16076 c        14591 g        14460 t
ORIGIN      
        1 ttgccgggtt ttctcgtttt tggtcactta ctcatcaact catttcattt gttatatgaa
       61 tgtttcttac caccctcacg cgacaaatat catcacagtt aatatgtcat acaatttatg
      121 ttgcaacaca aacgtttccc tattttcata aaccgtattt tatcgctatg aaaaagaaat
      181 tatcgccatg attaactaaa agtattgatt ttttcagttc aacctacata tattgcgcgc
      241 cccggaagaa gtcagatgtc gtttaatggg caaatattgc ccttaaattc tcttttactt
      301 ttgatttaca gagtaaagcg ttgggataat ctatcttcca agcagattat tgtatttggg
      361 atcaagatca ctgatagata cataacttgt gtgtatcttt ccgccctcaa attattacgg
      421 cggtaaatga ttaagccatc gccgatagac agatttcatt tttacggtca ggcaccttcc
      481 cgggctgaac tggctaaaag ctgaattatt tgcattcctc caggagaaat agatggaaac
      541 gactcaaacc agcacgattg cgtcgaaaga ctctcgtagt gcctggcgca agacagacac
      601 catgtggatg ctgggccttt acggcacggc aatcggcgcg ggcgtgctgt tcctgccaat
      661 caacgccggt gttggcggta tgatcccgct gatcatcatg gctatccttg cgttcccgat
      721 gacgtttttt gctcaccgcg gcctgactcg cttcgtactg tctggtaaaa acccgggcga
      781 agacatcacc gaggttgtag aagaacactt tggtattggc gcaggtaaac tgattaccct
      841 gctctacttc ttcgctatct acccgatcct gctggtttat agcgtggcaa tcaccaatac
      901 cgttgaaagc ttcatgtctc accagctggg tatgacgcca ccgccgcgtg cgattctgtc
      961 gctgatcctg atcgtgggta tgatgaccat cgttcgcttt ggtgagcaga tgatcgttaa
     1021 agcgatgagt attctggtat tcccgtttgt tggcgtactg atgctgctgg ctctgtacct
     1081 gatcccgcag tggaacggcg cagcactgga aacgctgtct ctggacactg catctgcaac
     1141 cggaaacggt ctgtggatga ccctgtggct ggcaattccg gtaatggtgt tctcgttcaa
     1201 ccactctccg atcatctctt ctttcgccgt tgcgaagcgt gaagagtacg gcgatatggc
     1261 agaacagaaa tgctcgaaga tcctggcatt cgcacacatc atgatggtgc tgaccgtaat
     1321 gttcttcgtc ttcagctgcg tactgagcct gactccggca gacctggctg cggctaaaga
     1381 gcagaacatc tcgattctgt cttacctggc taaccacttt aacgcaccgg ttatcgcgtg
     1441 gatggctccg attatcgcga ttatcgctat caccaaatcc ttcctcggcc actacctggg
     1501 cgcacgtgaa ggcttcaacg gtatggtgat taaatctctg cgtggtaaag gtaagtctat
     1561 cgaaatcaac aagctgaacc gtatcactgc gctgttcatg ctggtaacga cctggattgt
     1621 tgccaccctg aacccgagca tcctgggtat gattgaaacc ctgggcggcc caatcatcgc
     1681 gatgatcctg ttcctgatgc cgatgtacgc aattcagaaa gtaccggcaa tgcgtaagta
     1741 cagcggtcac atcagcaacg tattcgttgt cgtgatgggt ctgattgcaa tctccgcaat
     1801 cttctactct ctgttcagct aagtcctttc gcgccgcttt cgggcggcgc ttcctccgtt
     1861 ttaacgcgat gtatttccta tgattagcgt attcgatatt ttcaaaatcg gcattggccc
     1921 ttccagttct cataccgttg gaccaatgaa agcgggtaaa caatttaccg acgatctgat
     1981 tgcccgtaac ctgcttaaag acgtgacccg cgtggtggtt gacgtgtacg gctcgctctc
     2041 tctgactggt aaaggccacc acacagatat cgccattatt atgggcctgg cgggtaacct
     2101 gccggatacc gtggatatcg attccatccc cggttttatt caggatgtga atactcatgg
     2161 tcgcctgatg ctggcaaacg gtcagcatga agtggagttc ccggttgatc agtgcatgaa
     2221 cttccatgca gacaaccttt ctctgcatga aaacggtatg cgcattaccg cgctggcggg
     2281 cgataaagtc gtttacagcc agacttacta ctctattggc ggtggcttta tcgttgatga
     2341 agagcatttt ggccagcagg atagcgcacc ggttgaagtt ccttatccgt acagttcagc
     2401 agccgatctg caaaaacatt gtcaggaaac cgggctgtca ctctctggcc tgatgatgaa
     2461 aaacgagctg gcgctgcaca gtaaagaaga gctggaacag cacctggcga acgtctggga
     2521 agtcatgcgt ggcggtattg agcgcggtat ttccaccgaa ggcgtgttgc ctggcaaact
     2581 gcgcgttcca cgccgtgctg cggcactacg ccggatgctg gtcagccagg ataaaaccac
     2641 cactgacccg atggcggttg ttgactggat caacatgttt gcactggcag tgaacgaaga
     2701 gaacgctgct ggcggtcgcg tggtgactgc gccgactaac ggtgcgtgcg ggattatccc
     2761 ggctgtgctg gcgtactacg ataagtttat ccgcgaagtg aacgctaact cactggctcg
     2821 ttacctgctg gtagccagcg ccattggttc tctttataag atgaacgcgt cgatttctgg
     2881 tgccgaagtg ggttgccagg gtgaagttgg cgtggcgtgc tctatggcgg cagctggtct
     2941 ggcagaactg ttaggcgcaa gcccggcgca ggtttgcatc gcagcggaaa tcgcgatgga
     3001 gcacaacctc ggtctgactt gtgacccggt cgcggggcag gttcaggtac cgtgcattga
     3061 gcgtaacgcc attgcagcag tgaaagcggt gaacgccgcg cgtatggcac tgcgccgtac
     3121 cagcgaaccg cgcgtctgcc tcgataaagt tatcgaaacc atgtacgaaa caggtaaaga
     3181 catgaacgcc aagtaccgcg aaacctctcg cggcggcctg gcgatgaaga tcgttgcctg
     3241 cgattaatct ctcccagagg cctcgttttg cgaagcctct tcctgttttc tcaaccagcc
     3301 atagcctgtt ccggcatcga atgttaccct tatcgcctga tctttaaggg ggttatcgtg
     3361 gctgttcatt tgcttattgt cgatgcactg aatcttatcc gtcgcattca tgccgttcag
     3421 ggttcgccct gtgtcgaaac ctgccagcat gcgctcgatc agctcattat gcacagccag
     3481 ccaacccacg cggtcgccgt ttttgatgat gaaaaccgca gtagcggctg gcgtcatcag
     3541 cgtttaccag attacaaagc ggatcgaccg ccaatgccgg aagagttgca cgacgagatg
     3601 cctgcattac gcgccgcctt tgagcaacgc ggcgtcccgt gctggtcagc cagcggcaac
     3661 gaagccgatg acttagccgc cacactggcg gtcaaagtga cacaggccgg gcatcaggca
     3721 acgattgttt cgacagataa aggctactgt cagttacttt caccgacatt acgtattcgt
     3781 gattacttcc agaaacgttg gctggatgcg ccatttatcg ataaagaatt tggcgttcaa
     3841 ccgcagcagt tgcccgatta ctggggactt gcggggatca gcagttcaaa ggtaccgggt
     3901 gttgcgggaa tcggaccaaa aagcgccacg cagctgctgg tcgagtttca gagtctggaa
     3961 gggatatatg agaatctgga tgcggttgcc gaaaagtggc gcaaaaaatt agaaacccat
     4021 aaagagatgg cgtttctgtg ccgcgatatt gcccgcttac aaaccgattt gcatatcgac
     4081 ggcaatttac agcaattgcg gttggtacgg taacggcgag ccggatacgc cgcaaatcgt
     4141 atccggcatt atcacatcag cgcatttacc aggcggtatg gtaaagctct acaatatcct
     4201 caagcgttgc ttcacgcggg ttgccaccgg tacaaacatc atccagtgcc gcctgagcca
     4261 gtgccgaaat gtcttccttg cgtacaccaa catcacgcaa atgtggcgga ataccgacat
     4321 cacggttgag ggcaaacacc gcttcaacag cggcattacg cgccccttcc aggctcatac
     4381 cttccacttt cacgcccata acgcgcgcga tatcgcggta cttctcaccg gtaaagtcag
     4441 cgttatagcg catgacatgc ggtaacagga tggcgttcgc aacaccgtgt ggagtgttat
     4501 aaaacgcgcc cagtggatgc gccataccat gcaccaaccc taacccaaca ttcgagaagc
     4561 ccatacccgc aacatactgc ccgagcgcca tttcttctcc ggcatcctta tcaccagcaa
     4621 ccgatcctcg cagcgcccca gcaatgattt caatcgcttt aatgtgcagt gcatcggtta
     4681 gtgcccacgc gccacgggta atatacccct caatagcatg agtgagcgca tcaacacccg
     4741 tcgcggcttt cagcgccgga ggcataccat ccatcatgtc agcatcaata aacgccacct
     4801 gcgggatatc atgcggatca acgcaaacaa acttgcgccg tttttcttcg tcagtgatca
     4861 cgtagttaat ggtcacttct gccgcagtac ctgctgtggt ggggattgcc agaatcggta
     4921 cactgggttt attggtcggg gaaagccctt ccaggctacg cacatcggca aactccgggt
     4981 tgttgctgat aatgccaatt gctttacaag tatcctgtgg agaaccacca ccaatagcga
     5041 tcaggtaatc cgcgccgcta ttctggaata caccgagccc ttctttgacg acagtaattg
     5101 ttgggttggg cactacgccg tcgtaaatcg cccatgccag ccctgcagca tccatcttat
     5161 cggtcacttt cgccaccacg ccgcattgca ccagcgtttt atcggtgacg atcagcgcct
     5221 tctgataacc acggcgtttc acctcatcgg ttaaagcccc aacagcaccc cgaccaaacc
     5281 atgccgtttc gttcagaatc attctgttag ccatcatcct tctccttgtt gctttacgaa
     5341 attactcttc aatccgtaac ccataggttt tgaatttctc cagcactacg gcaatctctt
     5401 catcgctcag cactggcacc gggtccgtaa tcgccagggt cgtcaggtaa agttgcgcca
     5461 gcacttcaac ttcatgcgcc agccataacg ctttttccag attcacctca caagcgataa
     5521 gcccatgatg ttgtaacaaa gttgccttac gatttttgag agccagcgca acatgttcag
     5581 aaagttcgcg tgttccaaag gtcgcataag gcgcgcaagg aatagaatta ccgccagccg
     5641 ccgcaatcat gtagtgaata gcggggatcg atcggttaag aatggaaact gccgtgcaat
     5701 gaacggcatg attgtgaaca accgcgttgg catccggtct gctttgatag gctgccatat
     5761 ggaaacgcca ttcgcttgag gggagctttc cttcctcatg tttaccgttg ccatcaataa
     5821 agacaatatg cgactccgtc agtttttcat atggaatgcc tgtaggcgta atcagcatcc
     5881 catcctgata acgtacactg acgttccccg ctgtcccctg gttcagtccc aggcgggtca
     5941 tttccaggca agtgtcaata atctgacgag caagtttatt tcgttccatt agctacctct
     6001 ctctgattca aaacagggca ataatgttgt tcctttcaca ctattgaatt agccgtttaa
     6061 ttacccacca tcttcttcct gattaacaag aaagaaattc acaagcttat attttgtgac
     6121 ctagctcaac taatcacagt aaataactgc aagttctctt tttataaccc cgttaaaaat
     6181 gaccgctctt aaaaatattt atcaaaacgg tcatttttcc atttcccctc gccagaaatg
     6241 cccgttttcg gcacaaacaa ttaatacggt catctggttt gtgtttttta tgatttattt
     6301 tctgaaacgg gcatgaaatt tcgattatta aagtgatggt agtcacataa agtcaccttc
     6361 cagctaataa gtgtgaccgc cgtcatatta cagagcgttt tttatttgaa aatgaatcca
     6421 tgagttcatt tcagacaggc aaatattcac cgatatgaag cccgaactcg ctggttttgc
     6481 acttttgaaa acataaccga ttacgtgctt aagcttctga acctaagagg atgctatggg
     6541 aaacacatca atacaaacgc agagttaccg tgcggtagat aaagatgcag ggcaaagcag
     6601 aagttacatt attccattcg cgctgctgtg ctcactgttt tttctttggg cggtagccaa
     6661 taaccttaac gacattttat tacctcaatt ccagcaggct tttacgctga caaatttcca
     6721 ggctggcctg atccaatcgg ccttttactt tggttatttc attatcccaa tccctgctgg
     6781 gatattgatg aaaaaactca gttataaagc agggattatt accggattat ttttatatgc
     6841 cttgggcgct gcattattct ggcccgctgc agaaataatg aactacacct tatttttagt
     6901 tggcctattt attattgcag ccggattagg ttgtctggaa actgccgcaa acccttttgt
     6961 tacggtatta gggccggaaa gcagtagtca cttccgctta aatcttgcgc aaacatttaa
     7021 ctcgtttggc gcaattatcg cggttgtctt tgggcaaagt cttattttgt ctaacgtgcc
     7081 acatcaatcg caagacgttc tcgataaaat gtctccagag caattgagtg cgtataaaca
     7141 cagcctggta ttatcggtac agacacctta tatgatcatc gtggctatcg tgttactggt
     7201 cgccctgctg atcatgctga cgaaattccc ggcattgcag agtgataatc acagtgacgc
     7261 caaacaagga tcgttctccg catcgctttc tcgtctggcg cgtattcgtc actggcgctg
     7321 ggcggtatta gcgcaattct gctatgtcgg cgcacaaacg gcctgctgga gctatttgat
     7381 tcgctacgct gtagaagaaa ttctaggtat gactgcaggc tttgccgcta actatttaac
     7441 cggaaccatg gtgtgcttct ttattggtcg tttcaccggt acctggctca tcagtcgctt
     7501 cgcaccacac aaagtcctgg ccgcctacgc attaatcgct atggcactgt gcctgatctc
     7561 agccttcgct ggcggtcatg tgggcttaat agccctgact ttatgcagcg cctttatgtc
     7621 gattcagtac ccaacaatct tctcgctggg cattaagaat ctcggccagg acaccaaata
     7681 cggttcgtcc ttcatcgtta tgaccattat tggcggcggt attgtcactc cggtcatggg
     7741 ttttgtcagt gacgcggcgg gcaacatccc cactgctgaa ctgatccccg cactctgctt
     7801 cgcggtcatc tttatctttg cccgtttccg ttctcaaacg gcaactaact gaacatattt
     7861 tccgaataaa gtgaggaatc tgtaatgaaa aaaatcagct taccgaaaat tggtatccgc
     7921 ccggttattg acggtcgtcg catgggtgtt cgtgagtcgc ttgaagaaca aacaatgaat
     7981 atggcgaaag ctacggccgc actgctgacc gagaaactgc gccatgcctg cggagctgcc
     8041 gtcgagtgtg tcatttccga tacctgtatc gcgggtatgg ctgaagccgc tgcttgcgaa
     8101 gaaaaattca gcagccagaa tgtaggcctc accattacgg taacgccttg ctggtgctat
     8161 ggcagtgaaa ccatcgacat ggatccaacc cgcccgaaag ccatttgggg ctttaacggc
     8221 actgaacgcc ccggcgctgt ttacctggct gcggctctgg cagctcacag ccagaaaggc
     8281 atcccggcat tctccattta cggtcatgac gttcaggatg ccgatgacac atcgattcct
     8341 gccgatgttg aagaaaaact gctgcgcttt gcccgcgccg gtttggccgt cgccagcatg
     8401 aaaggtaaaa gctatctgtc gctgggcggc gtttcgatgg gtatcgccgg ttctattgtt
     8461 gatcacaact tctttgaatc ctggctggga atgaaagtcc aggcggtgga tatgaccgaa
     8521 ctgcgtcgcc gtatcgatca gaagatttac gacgaagccg aactggaaat ggcactggcc
     8581 tgggctgata aaaacttccg ctatggcgaa gatgaaaata acaaacagta tcaacgcaat
     8641 gccgagcaaa gccgcgcagt tctgcgcgaa agtttactga tggcgatgtg tatccgcgac
     8701 atgatgcaag gcaacagcaa actggccgat attggtcgcg tggaagaatc acttggctac
     8761 aacgccatcg ctgcgggctt ccaggggcaa cgtcactgga ccgatcaata tcccaatggt
     8821 gacaccgccg aagcgatcct caacagttca tttgactgga atggcgtgcg cgaacccttt
     8881 gtcgtggcga ccgaaaacga cagtcttaac ggcgtggcaa tgctaatggg tcaccagctc
     8941 accggcaccg ctcaggtatt tgccgatgtg cgtacctact ggtcaccaga agcaattgag
     9001 cgtgtaacgg ggcataaact ggacggactg gcagaacacg gcatcatcca tttgatcaac
     9061 tcaggttctg ctgcgctgga cggttcctgt aaacaacgcg acagcgaagg taacccgacg
     9121 atgaagccgc actgggaaat ctctcagcaa gaggctgacg cttgcctcgc cgctaccgaa
     9181 tggtgcccgg cgatccacga atacttccgt ggcggcggtt actcttcccg cttccttacc
     9241 gaaggcggcg tcccgttcac catgactcgt gtcaacatca tcaaaggcct gggaccggta
     9301 ctgcaaatcg cggaaggctg gagcgtggaa ttgccgaagg atgtgcatga catcctcaac
     9361 aaacgcacca actcaacctg gccaaccacc tggtttgcac cgcgcctcac tggtaaaggg
     9421 ccgtttacgg atgtgtattc ggtaatggcg aactggggtg ccaaccacgg agttctgacc
     9481 atcggccacg ttggcgcaga ctttatcacc cttgcctcca tgctgcgtat cccggtatgt
     9541 atgcacaacg ttgaagagac caaagtgtat cgtccttctg cctgggctgc gcacggcatg
     9601 gatattgaag gccaggatta ccgcgcttgc cagaactacg gtccgttgta caagcgttaa
     9661 tacattacac cctgatgtga tgcccggtcg ctccggctac cgggcctgaa caagcaagag
     9721 tggttagccg gataaacaat gttatccggc tatattgcag gagcgattat gaaacaagaa
     9781 gttatcctgg tactcgactg tggcgcgacc aatgtcaggg ccatcgcggt taatcggcag
     9841 ggaaaaattg ttgcccgcgc ctcaacgcct aatgccagcg atatcgcgat ggaaaacaac
     9901 acctggcacc agtggtcttt agactgccat tttgcaacgc tttgctgatt gctgtcggca
     9961 aatcaatagt gaactgactg aatgccacat ccgcggtatc gccgtcacca cctttagtgt
    10021 ggatggcgct ctggtagata agcaaggcaa tctgctctat ccgatcatta gctggaaatg
    10081 tccgcgaaca gcagcggtta tggacaatat tgaacggtta atctccgcac agcggttgca
    10141 ggctatttct ggcgtcggag cctttagttt caatacgtta tataagttgg tgtggttgaa
    10201 agaaaatcat ccacaactgc tggaacgcgc gcacgcctgg ctcttcattt cgtcgctgat
    10261 taaccaccgt ttaaccagcg aattcactac tgatatcacg atggccggaa ccagccagat
    10321 gctggatatc cagcaacgcg atttcagtcc gcaaatttta caagccaccg gtattccacg
    10381 ccgactcttc cctcgtctgg tggaagcggg tgaacagatt ggtacgctac agaacaacgc
    10441 cgcagcaatg ctcggcttac ccgttggcat accggtgatt tccgcaggtc acgataccca
    10501 gttcgccctt tttggcgctg gtgccgaaca aaatgaaccc gtgctctcct ccggtacatg
    10561 ggaaatttta atggttcgca gcgcctaggt tgatacttcg ctgttaagtc agtacgccgg
    10621 ttccacctgc gaactagata gccaggcatg gttgtataac ccaggtatgc aatggctggc
    10681 atccggcgtg ctggaatggg tgagaaaact gttctggacg gctgaaacac cctggcaaat
    10741 gttgattgaa gaagctcgtc tgatcgcacc tggcgcggat ggtgtgaaaa tgcagtgtga
    10801 tttattgtcg tgtcagaacg ctggctggca aggagtgacg cttaatacca cgcgggggca
    10861 tttctatcgc gcggcgctgg aagggttaac tgcgcaatta cagcgcaatc tacagatgct
    10921 ggaaaaaatc gggcacttta aagcctatga attattgtta gtcggtggag gaagtcgcaa
    10981 cacattgtgg aatcagatta aagccaatat gcttgatatt ccggtaaaag ttctcgacga
    11041 tgccgaaacg accgtcgcag gagctgcgct gttcggttgg tatggcgtag gggaatttaa
    11101 cagcccggaa gaagcccgtg cacagattca ttatcagtac cgttatttct acccgcaaac
    11161 tgaacctgaa tttatagagg aagtgtgaaa tgctgaaaac aatttcgccg ttaatttctc
    11221 ccgaactatt gaaagtgctg gcagagatgg gacatggaga tgaaattatt ttttccgatg
    11281 ctcactttcc cgcccattcg atggaaccgc aggtgatccg cgctgatggc ctgttggtga
    11341 gcgacttgct ccaggcgatt atcccgttat ttgagctgga cagttatgcc ccgccgctgg
    11401 tgatgatggc ggcggtagag ggtgacactc tcgatcctga agtagaacga cgttaccgta
    11461 atgcgctttc actacaagcc ccgtgtcctg acatcatccg catcaatcgt tttgcgtttt
    11521 atgaacgggc gcaaaaagcc tttgcgatcg ttatcacagg cgaacgagcg aagtacggga
    11581 atattctttt aaaaaaaggg gtaacaccgt aatctcatac cggtacgccc gcatgacgcg
    11641 ggcggttatc gaatgatggg gtgaaaaata tgaaagcggc acgccagcaa gcgatagtcg
    11701 acctgctgct gaaccatacc agcctgacca cggaagctct ctctgaacag ctaaaggtca
    11761 gtaaagaaac cattcgtcgc gatctcaatg aattacagac gcagggtaaa attctgcgca
    11821 atcatggacg cgctaaatat atccaccgtc aaaatcaaga cagtggcgat ccctttcaca
    11881 tcaggctgaa aagccattat gcgcataaag cagatatcgc gcgcgaggcg ctcgcgtgga
    11941 ttgaagaagg gatggtgata gccttagacg ccagttcaac ttgctggtat ctggcacgcc
    12001 agttgcctga catcaacatt caggtcttca ccaatagcca tccgatttgc catgaactcg
    12061 gtaaacgcga acgcattcaa ctgatcagtt ccggcggcac acttgagcgc aaatatggct
    12121 gttacgtcaa tccctcgctg atttcccaac ttaaatcgct ggaaatcgat ctgtttattt
    12181 tttcttgtga agggatcgat agcagcggcg cactgtggga ctccaatgcg atcaacgctg
    12241 attacaaatc gatgctatta aaacgtgccg cgcaatcgtt gttattgatt gataaaagta
    12301 aatttaatcg ttcaggggaa gcccgcatcg ggcatctgga tgaggtaacg cacattattt
    12361 ctgatgagcg ccaggttgca acttctttgg taacagcctg acggaaaggg tagcaggccg
    12421 gagacgacgc cccggccttg cctgttatcg ctcgtcgcga cgaccaccca ccgcagccca
    12481 gatgcggcgg acgtgcaccg tcacttcttc gcgatcgtga tacaactgcc gcgcctgaat
    12541 ctgagcattt atgccatgtt catcaagctg tgcctgaata tacgccagat tgtgtgatac
    12601 ttcttcgtag cgttttttca tcggcagttt gagattgaaa atggtttcac ggcaccagcc
    12661 attaaccagc cactgcgcca tcaatgccgc aactttagcc ggtttttcaa ccatatcgca
    12721 taccatccag gagatattgc tgcgtgtcgg acggaatttg aaaccgtctt cccgcagcca
    12781 cgtcacctgc ccggtatcca tcagactttg cgccatcggg ccgttgtcga cggaataaac
    12841 ccacatgttg cgcttcacca gttggtaggt ccagccgcca gggcaagcgc ctaaatccac
    12901 cgcccacatc ccgttcgcca ggcgttcatc ccactcatcc gcaggaataa acacatgaaa
    12961 tgcctcttcc agtttgagcg tggaacgact cggcgcctct gccggaaatt tcaggcgcgg
    13021 aatgcccata tagaacggcg aattattgtt gctgtatgag taaccggtat agcagcatcc
    13081 tggtgcaata aagaatacat gcacaaccgg acgcttcggc gtttcatagt tcgccagcac
    13141 cccagcatcg cgcaaggcag cgcgtagcgg aacagtgaac ttacggcaga atttcagcaa
    13201 ctctttgctt tcgttggtat cggcaacttc aacacgcagt tcaccgccct tctctactac
    13261 accctgtaac atgccgacaa tgggggtaat acgatcttct ggcggcaaat gctgcaggag
    13321 ttcccccacc acaaaccact ggcgggcaaa aattaatgaa ctgaacggca gctcacggat
    13381 taacttatcg ccatcatcag gttgataaca ttcataaatg acataacccg cattctcttt
    13441 cacgcgggca aaaccgaaaa tttcccgctg gccggcttta tcggtaattt ctgcggcgca
    13501 ctctttttca aagcccggac ggcacagcaa tacaacctta ttcatgactt acgcccttgc
    13561 gctttaaacg gatagcacca actaacatta acgcccagcc cgcaaggaag ctcacgccgc
    13621 caaccggtgt gacaaacgcc cacaaacgca gatgggacag cgccaggcaa taaaggctgc
    13681 cgctgaacag caccgtgcct aacgcgagga aaacgctact ccagtaaaac cagatactga
    13741 tgcgacgctg cattgccacc gccagaccta agatcgccag cgtatgaaac gcctggtatt
    13801 cgagaccggt ttggatccag cccatctcaa cggcccccat ggttttactt aacacatgcg
    13861 caccaaaagc gcccagtgcc acaaaaatga agccgctaat ggcggcgaaa atcagcataa
    13921 aacggctggt catggtcgta ccctacgtaa attattgttc ataacgaaag cggaattttt
    13981 cttgttcagc agcggctttc gccaggatcc attggcgaaa ggcggctatt ttacccagtt
    14041 ctgcctggct gtcatgacaa accagataaa aagcattttt actgaccaga acatcattaa
    14101 acgggcaaac aagacgtccg gcctcgattt cagattgcgc catcacgtta tttgccagcg
    14161 ccactccctg cccgtgaata gccgcttgca gcaccatggc gctatgacta aaaattggcc
    14221 cttgctgaac gttgatatga ttaaacccca actgtcgggt atatgtctgc cagtcacggc
    14281 gtgacgcatc atgtaataac gtatgtttag ccagatcttc cggggtcttc aagggttttt
    14341 cgcctgtcag cagtagcggc gaacacaccg gcaataaata ttcggcgtac agtttttcca
    14401 cccgtagccc cggccagttg ccccgaccat aaaatatcgc cacatcaaca tcatccgcca
    14461 gcttatcttc ctgacgatca accgcctgga ttcgaacgtc aattcccgga taagctgaat
    14521 taaagctgga aagtcgcgga accaaccaat gaatggcgaa actggggagt aaactgaccg
    14581 tcaacgcccc cttggcgcta cgggcctgga gtttacgcgt cgcttcggtt aattgcgaaa
    14641 atatctcttt gatatcgagg aaatagcttt gcccttcctc ggtcagcagg agtgaacgat
    14701 tacggcggcg gaacagtttt agccccaaaa aatcctcaag agacttgatt tgatgactta
    14761 ctgcggcctg ggtcacaaaa agctcttctg ctgcgcgagt aaaacttaaa tggcgtgctg
    14821 cggcatcaaa aactcgtaag gcatttagcg gtggtaatcg tttagacatg gcttttaaac
    14881 tttgatgtta aatgaattta acaattagat cacactgtgt aacctattag tttttttaat
    14941 ctgagccatt ataaattgtc cgttgagctt ctaccagcaa atacctatag tggcggcact
    15001 tcctgagccg gaacgaaaag ttttatcgga atgcgtattc tggtgaactt ttggcttacg
    15061 gttgtgatgt tgtgttgttg tgtttgcaat tggtctgcga ttcagaccat ggtagcaaag
    15121 ctaccttttt tcacttcctg tacatttacc ctgtctgtcc atagtgatta atgtagcacc
    15181 gcctaattgc ggtgcttttt tttaccttgc gatcgcgaat tactgttcca gttcgaccat
    15241 ctctttcacg tcagtacggt tgatctgctg tttgttgcca ttagcgtctt tatacgaaat
    15301 cataccgata tcgttatcag tctgtggttt gccgtcagag acgatggtac gtccgtcatt
    15361 ggtgtgcatc acatagttcg aaccggaaca ggcgctcagg gcaaaagtca gcatacaggc
    15421 agaaataatt gcggcagtct ttttcatcat cttctcctta aaacgattaa taaatcttaa
    15481 tagtattgat taacaggcta aaattaacgc ctaacactat tcagcatatg ttacttacgc
    15541 ggcttcgcca ggatatccag ataattctga tggttagcac tctccttgta tcaaagtgaa
    15601 ttttgcgtca cgatcggtgc atcaagccga ggagtaccat gaacgttttt aatcccgcgc
    15661 agtttcgcgc ccagtttccc gcactacagg atgcgggcgt ctatctcgac agcgccgcga
    15721 ccgcgcttaa acctgaagcc gtggttgaag ccactcgaca gttttacagc ctgagcgccg
    15781 gaaacgtcca tcgcagccag tttgccgaag cccaacgcct gaccgcgcgt tatgaagctg
    15841 cacgagagaa agtggcgcaa ttactgaatg caccggatga taaaactatc gtctggacgc
    15901 gcggcaccac tgaatccatc aacatggtgg cacaatgcta tgcgcgtccg cgtctgcaac
    15961 cgggcgatga gattattgtc agcgtggcag aacaccacgc taacctcgtc ccctggctga
    16021 tggtcgccca acaaactaga gccaaagtgg tgaaattgcc gcttaatgcg cagcgactgc
    16081 cggatgtcga tttgttgcca gagctgatta ctccccgtag tcggattctg gcgttgggtc
    16141 agatgtcgaa cgtgactggc ggttgcccgg atctggcgcg agcgattacc tttgctcatt
    16201 cagccgggat ggtggtgatg gttgatggtg ctcaggggac ggtgcatttc cccgcggatg
    16261 ttcagcaact ggatatcgat ttctatgctt tctctggtca caaactgtat ggcccgacgg
    16321 gtatcggcgt gctgtatggc aaatcagaac tgctggaagc gatgtcgccc tggctgggtg
    16381 gcggcaaaat gattcacgaa gtgagttttg acggtttcac cactcaatct gcgccgtgga
    16441 aactggaagc aggaacgcca aatgtcgctg gcgtcatagg attaagcgcg gcgctggaat
    16501 ggctggcaga ttacgatatc aaccaggccg aaagctggag ccgtagctta gcaacgcttg
    16561 cagaagaagc gctggcgaaa cgtccaggct ttcgttcatt ccgctgccag gattccagcc
    16621 tgctggcctt tgattttgct ggcgttcatc acagcgatat ggtgacgcta ctggcggagt
    16681 acggtattgc cttgcgggcc gggcaacatt gcgctcagcc gctactggca gaattaggcg
    16741 taaccggcac actgcgcgcc tcatttgcgc catataatac aaagagtgat gtggatgcgc
    16801 tggtgaatgc cgttgaccgc gcgctggaat tattggtgga ttaatgacaa acccgcaatt
    16861 cgccggacat ccgttcggca caaccgtaac cgcagaaacg ttacgcaata ccttcgcacc
    16921 gttgtcgcaa tgggaagata aatatcgcca gttgatcatg ctggggaaac agcttccggc
    16981 tttgccagac gagttaaaag cgcaggctaa agagattgcc ggatgcgaaa accgcgtctg
    17041 gctgggatat accgtggctg aaaacggcaa aatgcacttc tttggcaaca gcgaagggcg
    17101 cattgtgcgc ggcctgttgg cggtgttgtt gactgccgtt gaggggaaaa ccgccgccga
    17161 attacaggca cagtcgccgc tggcattgtt tgatgagctg ggattacgtg cgcagcttag
    17221 cgcctcacgc agccaggggt taaatgcgtt aagcgaggcg attatcgctg cgacgaagca
    17281 ggtttaagca cagaaccggg tcggataaga cgttcgcgtc tcatccgacc tgattgttta
    17341 accctgacgc gccgctttcg ccatcatctt cttcagcgca tgagaaaccg caacaaaccc
    17401 aaaggtggcg gtcaccatcg ttgccgcgcc aaatccagat gcacaatcca tccgcttcgg
    17461 cccttctgcc gtggctttca tcgcacacac cgtaccgtct gactgcgggt acaccagcgc
    17521 ttcagtagaa aacacgcaat ccacgccgag cttaccttta ctgtttttca ctacgccaaa
    17581 atcgcttttc aggcgctcgc gcaacttcgc cgccagcgga tcctgaatcg ttttcgccag
    17641 atcggtaacc tgaatctgcg tcgggtcaat ctgcccaccc gcgccaccgg tcgtgaccag
    17701 cgggatttta ttgcgccgac aataagcaat cagcgccgct ttgggccgta cactatcaat
    17761 ggcatcaatc acgtacgaat aaccggcact catatattgc gctacgttat ccggcgtcac
    17821 gaaatcatcc accaccgtta cacggcactc cgggttaatc tggcgaatgc gctccgccat
    17881 gacttccgct tttgccagcc cgacgttatc gcgcagagcg tgaatttgcc gattggtatt
    17941 ggtgacgcac acatcatcca tatcgataag cgtgattgcg ccaatccccg tgcgcgccag
    18001 cgcttccgcc gcccaggaac cgacaccacc gataccaacc acacaaatat gcgcgtcagc
    18061 aaacagttgc aacgcttttt caccatacag acgcgctgtg ccaccaaaac gctgacgcca
    18121 tgcatcacta attaccacag acatagcacc tcagaattaa aaagggcgag gttatccctc
    18181 accctaaacg cataaatgcc tgatgcgcta cgcttatcag gcctacataa ctcctgcaat
    18241 attttggatt tgcatgattt gtaggccgga taaggcgttc acgccgcatc cggtatgaac
    18301 aacgcgcact tagtcaataa tcaggatgtg aggttacccc tcccctgtca tatccgtaaa
    18361 aacggcatac agaatatcac atcagccgct aaagacgtta cctgcgcccg gagccgtttt
    18421 cagcacccaa acccgtccat agtggttgta ccaacctgcg cggtgtccgg cttccggccc
    18481 gatcccttga tagatatcga agtgttggcc tttgattgcg cccccgacat ccaacgccac
    18541 catcagacgc aattcgtact gaccgttaaa tttgccgtta ttatccagca gcggcacttc
    18601 tgccagcaag gtagtacctg gcggaataat tgaacgatcg gaggcaacag aagcacggcc
    18661 aaccagcggc accgcacttg cccctttcac cggtgcaaaa gattgcggtt taaagaagac
    18721 gaaagacggg ttctgttcca gcagctcgcg aacctcggct tcactgtgtg tttcgcccca
    18781 atgacgaatc gcctgcatcg acatatcttc ttttttcact tcgccacggt cgatcagcac
    18841 cttaccaatg ctgcgatagg catgaccgtt tttccctgca tagctgaaaa agttaagcgg
    18901 actgccatca ccaaagtcga tatacccact accctgcaca tccataatga agttatccat
    18961 cagagagttg ctgtaagcga ggatgtattt atcgcccaat gccccggcgt agatctccgc
    19021 acgagacggc agacgaccac gttttggcgg catacggtaa ataggatact ggaactcgcc
    19081 ctggcgggta tggcgcgcct gaattaccgg cgtgtaatag cctgtaaact gcacgttacc
    19141 atagttgtcg gcaccttcca tctgccaggc atcaatgccg aactggcgca tattgcgggt
    19201 atcaccgcct gcgcgcagcc actcttgcac cgcgttataa acattactct ggttgccata
    19261 cagacgcggt gacgaattac ggatatggtt aatttgctcg acaaaatcac cggcgttaat
    19321 cggcgcgcca acggcatctg gctggttcac cagagagaaa ggctgggtaa atttcccgtc
    19381 tttatattgc tgtccgcgat cggttggttt ggaagagcag gcggcaagca ttgccacaac
    19441 cgtgcccata agaaggtact ttacccaacg tcctttcatt gttctcttct tcaggttaaa
    19501 aaataaggcg caacgaagat aacaaaccgc cgcgtttaat gaaatgagca gcctctccct
    19561 gacgcaaatt ttgcacaaaa aataggcttt agtggtttgt ttttgttcaa aatcatgcta
    19621 aatccgtgat cggggtaaaa aaaaggttgc atgaaaacgc gagcggagta tagtgcgcat
    19681 ccacggacgc ggggtggagc agcctggtag ctcgtcgggc tcataacccg aaggtcgtcg
    19741 gttcaaatcc ggcccccgca accaatcaaa tttgatgaag taaaagcagt acggtgacgc
    19801 ggggtggagc agcctggtag ctcgtcgggc tcataacccg aaggtcgtcg gttcaaatcc
    19861 ggcccccgca accaattatt gaacacccta acgggtgttt ttttgtttct gatctcccat
    19921 aaaaaaagcg ccattcagcg cctttttatc atccccttct cgccagcgtt gccccatcgg
    19981 caaaatacgc tttaatcccc gcaagaatag actccgcaac ttcctgctgg aaagtcgccg
    20041 ttttcagttt acgctcttcc tcaacgttac tgataaacgc cgtttcgacc agaatggagg
    20101 gaatatctgg tgcctttagt acggcaaacc cggcctgttc aacttgattt ttatgcagct
    20161 tgttgatttt tccgagctta ttcagtaccg ctttaccaaa cttaaggctg tcagcaatag
    20221 tcagcgactg caccatatcg aacatggtgt ggtcgacata gcggtcaccg cttttgctca
    20281 cgccaccaat caagtccgag gcgttctggg tttgtgccag atattttgcc gcagtactgg
    20341 ttgcgccttt ggttgagagc gcaaacaccg aggaaccgct cggctgacga ctggtaaagg
    20401 cgtcggcatg gatagagaca aacaggtcag cacgctgttt ctgggctttt gctacgcgca
    20461 cttgcaacgg aatgaagatg tcttcattgc gcgtcatgta caccttcata ttgccctctt
    20521 cctcgatcag agagcgcaaa cggcgagcta tttgcaatac cacatctttt tcgcgcgttt
    20581 tgtatttccc caccgcaccg gagtcttcgc caccgtggcc agggtcaagc ataatgacaa
    20641 tcggacgatc gcgccctgct ttacccggtt gtggaccact ttgcgctggc ggcacctgct
    20701 tttcgaggtc gcctttgttg taatcctcca gcagcgccag cagcgggtcc tgcatatcct
    20761 gtgcattggc cggatagagg tccatcacca gacgctcttt aaacccggcg accggcgcaa
    20821 gggcaaacag ctgcggtttt acgttttgct ttaattcaaa aaccatgcgt acggtttgcg
    20881 ggtcaaattg cccgacgcgc gccgacttga tgaacgggtc gtctgcacga atttgtgccg
    20941 ccatcccctt gagtaccgag ttcaggttta catcttcgat atccaccacc acgcgttcag
    21001 ggttactcaa cgcgaactgc ttatatttca gctgacgatt tgattctacc gtcacgcggg
    21061 tgtagctgga cgcaggccag acgcgcaccg ccacgacctg gctaaccgca gccaggctga
    21121 cctgacttac gctcaatagc cacatagcgc ccgcgccttg cagtaaacga cgacggctga
    21181 ttgcagtgtt ggatcctgac atgcctctcc cgagcaaaag aaatctaagc tgtgtaacaa
    21241 gtaaacgact aatttgaccg gaaactttag cgaaagacgc ataatctgtc atctaataaa
    21301 cggtaaacat tctttttata ttcacggcat tactgataaa aaagtcgctc tcgcataaaa
    21361 tttacacttg caccctgcga aaaaacagaa taaaaataca ataatttcaa ataatcatgc
    21421 aaaggggtgt gccgtggtaa aggaacgtaa aaccgagctg gtcgagggat tccgccattc
    21481 ggttccctat atcaataccc accggggaaa aacgtttgtc atcatgctcg gcggtgaagc
    21541 cattgagcat gagaatttct ccagtatcgt taatgatatc gggttgttgc acagcctcgg
    21601 catccgtctg gtggtggtct atggcgcacg tccgcagatc gacgcaaatc tggctgcgca
    21661 tcaccacgaa ccgctgtatc acaagaatat acgtgtgacc gacgccaaaa cactggaact
    21721 ggtgaagcag gctgcgggaa cattgcaact ggatattact gctcgcctgt caatgagtct
    21781 caataacacg ccgctgcagg gcgcgcatat caacgtcgtt agtggcaatt ttattattgc
    21841 ccagccgctg ggcgtcgatg acggcgtgga ttactgccat agcgggcgta tccggcggat
    21901 tgatgaagac gcgctccatc gtcaactgga aagcggtgca atagtgctaa tggggccggt
    21961 cgctgtttca gtcactggcg agagctttaa tctgacctcg gaagagattg ccactcaact
    22021 agccatcaaa ctgaaagctg aaaagatgat tggtttttgc tcttcccagg gcgttactaa
    22081 tgacgacggt gatattgtct ccgaactttt ccctaacgaa gcgcaagcac gggtagaagc
    22141 ccaggaagag aaaggcgatt acaactccgg tacggtgcgc tttttgcgtg gcgcagtgaa
    22201 agcctgccgc agcggcgtgc gccgctgtca tttaatcagt tatcaggaag atggcgcgct
    22261 gttgcaagag ttgttctcac gcgacggtat cggtacgcag attgtgatgg aaagcgccga
    22321 gcagattcgt cgcgcaacaa tcaacgatat tggcggtatt ctggagttga ttcgcccgct
    22381 ggagcagcaa ggtattctgg tgcgccgttc tcgcgagcag ctggagatgg aaatcgacaa
    22441 attcaccatt attcagcgcg ataacacgac tattgcctgc gccgcgctct atccgttccc
    22501 ggaagagaag attggggaaa tggcctgtgt ggcagttcac ccggattacc gcagttcatc
    22561 acggggcgag gttctgctgg aacgcattgc cgctcaggcg aagcagagcg gcttaagcaa
    22621 attgtttgtg ctgaccacgc gcagtattca ctggttccag gaacgtggat ttaccccggt
    22681 ggatattgat ttactgcccg agagcaaaaa gcagttgtac aactaccagc gtaaatccaa
    22741 agtgttgatg gcggatttag ggtaagcgat gaaaatcacc ggatgcggca ggcgtaacac
    22801 tcgtgcgtcg catccggcaa ttacgtttat tcccgcgaac taaacaacgc cgccagacca
    22861 ctgcgccgct cagtacgagt ggcgattgcc gcacttaata tacgctcatc ggcatacagc
    22921 gacagacgac gacgcgcgcg ggtcaccgcg gtgtatacca gctctcgcgt tactaccggc
    22981 gtgcgttggc tcggtaaaat caacgccgca tggtcgaact ccgatccctg cgatttatgt
    23041 accgtcatcg cccacgtagt ttcgtgctct ggcaagcgac tcggttgcac agacttaata
    23101 ttgccgtccg gcatcgcaaa ccagacgcgc gtcccctgcc cgcgatccag cgcaataccg
    23161 atatcgccat taaacaaccc aagcgcgctg tcattacggg caatcatcac cggtcggcct
    23221 tcgtaccaac gagagtgcgg atgacgatga attttgcgct tctgttgcat aaactgttca
    23281 attcgctcat tcagtccagc cacgccaaac ggcccttccc gcagggcgca caaaagctgg
    23341 tactcattga acgcctgaat gattaaatcc ggctcggcac gcgcttgcag cagatccaga
    23401 taacgtccgt aacccgcaag agcttcctca agcatcgcaa tataatcttc gccgctctgt
    23461 aaaagtcgtt tttcgatatc agtaaaatcc tgctgaaaaa cggttttcac tgccgtttta
    23521 tcaccacggt tgatcgccgc agctaactga ccaatgccag aatcgctgcc gaaacgatag
    23581 cttttttgca gcaggcagag actgtcgcgc aaagatgccg cttctgtgcc ggttcctgcc
    23641 ggaacgtgag tccccgtcag gcggcttagc tgcctggcac gctcggcggt aaagcccgcg
    23701 ttggcataag cgcagatatc gcccagcaca gccccagcct caaccgaggc cagttgatca
    23761 cgatcgccga gaaagatcac tcgcgcatga tctggcaagg cgtcgatcag tctcgacatc
    23821 ataggcagat cgatcattga cgcttcatct accaccagca catcaagatg cagcgggtta
    23881 ccggcatgat gacgtaaacg ctggctaccc ggctgcgcgc ccagcaatcg gtgcaaagtg
    23941 ctggcatctt ccggaatgcg tttcttttgt tcatcggtca gcggtaactg tcgcaaagcc
    24001 ttgccgagag attcggttaa gcgcgcggca gctttacccg ttggtgcagc cagacggata
    24061 cggcagcgtt cgccgtcggc catttgaatt aacgctgcca gcaacttcgc cacggtggtc
    24121 gttttaccgg tgcctgggcc gccggaaatc accgagatcc gccgcgtcag cgccactgcc
    24181 gctgcaactt tttgccagtt aatttcatcg cttactggaa aaagtttgtc cagggtttgc
    24241 gccagtagag cttcatcaac ctcaatggta tgattcacct cattgaagaa gcgcgctacc
    24301 gtccgctcgt tacaccacat gcgattcaaa taaagacgat cgccacagag gatcatcggc
    24361 gtgggttcat ctcccctgct gaccgcttga gaagccagca agcattcttc ccaattttgt
    24421 agctcaccga tttcactgac acaggtcgcc aacagcggat tcgacgcctc gttattttcc
    24481 agtcgtgaaa gcggcaaaca gacgtgtccc tctccggcat catgacttaa cagtgccgcc
    24541 gcgagggtga cggcaggatg ttcatctccc gccacggtca gggcaaattg cacatccagc
    24601 gggcgtagct gtttgtgctc cacagcttcc agtaattgct tttgcaattt cattacgcct
    24661 cctccagggt cataccggca aacatctcat ccatcagggc aatcaacccg gcgttgggtc
    24721 gggttgtgta aatcccttgt tgcggatgtt ctttatcaac gccacgcagg aacagataga
    24781 taacgccgcc aaagtgaaga tcatagtcgt aatcagcaat gcgatggcgc agataacgat
    24841 gcagcgccag ggtataaagc tgatattgca gatcatagcg gtgtgcctgc atcgctgctg
    24901 ccatagcctg ttgggtgtaa gccgaactgt cttcacccag ccagttggat ttatagtcga
    24961 gcaggtaata acgcccttcg tggcggaata ccaggtcgat aaagcctttt aacatgccgc
    25021 gtacctgcat gaactccagc ggcgggcagc cagcggatag cgggtcaaac tggcgaatta
    25081 gtgcatcaag ctggctggcg atgagcggtt cgctaatcgg caggtaaaac tccatctcca
    25141 cctgtttatc gcggtcggaa agctgactca ggctaacacc cgtttcattg agaggtgcct
    25201 ggaggacagc cgtgatccac tcggtcaaca ccggttccca ctgcgattca aagccgccga
    25261 gttccagttt ttcctgtacc cagttcgggt caaccggctg ggtaaaatcg aggtcttcaa
    25321 acaaactgtg caagaatgtc cccggtgacg caccgcgcgg gaactgatgc ggtgttaacg
    25381 tcggttcttc aacgacgctg accacgcctg cggcatcaac atccagccgt ggcatcagat
    25441 cctgagcgat accgtgacca cgctgctgca agccggagta gctggtgaca cgccagttat
    25501 cgccgggcaa tcgttgtagt gtcctcgcat tcagttctgc agttaaagca tcattaacct
    25561 gccagggctg gttatcacca gtttgtgccg tttgccaggc aatatcatca tcgcataacg
    25621 cttcaataca ggtgcgaagc cctgccgcat cttgcggttc ccctttttgc agcaaacgcc
    25681 cgagcgcact ttggtggacg tcggtgtcac cttttttatc gccacgacgg cgcaccagcg
    25741 gtgcaacgcc gagactgcaa tgccaaaccg aacgcgtcag cgccacgtaa agcaaacgca
    25801 gatcttccgc cagacgttcg acctccgcga ggtcgacgct ttctggcgca gcattaagat
    25861 ccaaaactgc ctcaaacgag tggcgatcgt gataaaacgc ctgatcctgg acgcggaaat
    25921 tggtaataaa tggcaaccag accagcggat attccagccc tttcgattta tggatcgtga
    25981 caatctgcac cagatgttta tcactttcga ggcgcatttg ttggctggag gcattactgt
    26041 ctggctcgag gatatgttgc gataaccagc gtaccagcgc atgttcactt tccagctgcg
    26101 ttccggcttc ttgaagcagt tcgctgatat gcaagatatc ggtaagacgc cgctcaccgc
    26161 ctgccgttgc cagcaagttt tctgcaatgt tacgcgccga catcagcgcc cgcagcatcg
    26221 gcataacgcc acgtttgcgc cagatttgcc gataaccatc gaactcttcg actaccgcat
    26281 cccacgcatg ttcgtcatta ttcagcgttt caatatccag cgcattcagc cccatcattg
    26341 acgttgccag cgcactgcgc agggtgttct cacgttcggg cgtcatcacc gcctgcaaca
    26401 accaaagcat ttcctgcgct tccagagttt caaaaacact gtcgcggttc gaaaggtaaa
    26461 cggaagggat ttccagcaac gttaaggcat cgcgcacctg ggcggcctcc tggcggctgc
    26521 gcaccagcac gctgatgtcc gaagcacgca ccggacgcgc gtcgtcgccg ttcatcagca
    26581 acgcttcgcc ccgctgtccg gcttgtagcc agtcgcggat ttgcgcagca catacctgcg
    26641 ccatggtact ttgatagtcg ccaacgccgc agctttcgcc ttccatcagc cacattttca
    26701 tcgcaggctg cgtttcacct ttaaatacaa aacgtaacgc ctgatttttc ccggctgatt
    26761 tcactggaat aaacggtatt tcgcgaaaca tgaacgtgtc atcagtctgg ctgaaaagct
    26821 tgttcacact gttcaccatt ccaggtgcgg agcgccagtt ggtgtctaaa gtgtagtggg
    26881 catgaacttc actacgcgcc ttcatataag tgaagatatc cgcaccccgg aatgcatata
    26941 tggcctgctt cgggtcgcca atcagcaaca atgcggtttc cggctgatgg tgccagatac
    27001 ggcgaaaaat tcggtactgc tgggggtcgg tatcctgaaa ttcatcgatc attgccaccg
    27061 ggaatcgcgt acggatcgcc gctgccaaca cctcgccgct ttcgctacgc agcgcggaat
    27121 cgagccgact taacatgtca tcaaaaccca attcgccacg gcggcgtttt tcacgcgcta
    27181 ctgtttcgcg gatctcagcc aatgcgcggg tgatcaacag atcgcggatc gacaatggtt
    27241 ctgcaagcag ttgatcgatc gcctcaaaca gtggatgtcg cggggtttcc cccccggcct
    27301 tcgtgcgatc ttctaagaaa cgctgggaga atttttccag cgactccggc aactgataac
    27361 tgtttgtctc ttcttctgcc caggcgctga tcttttcgat ccatttagcc tgattgctac
    27421 ggttaaactt gcgtcgatca ataccagaag attcgatcag cgcatccagt tcacccactg
    27481 cgtcgcgcca ctgctgtttt accgtatcaa tacgcgccac aatttgcgcg tggcgggaag
    27541 ccagcgtttc atcatcgggc ggcggtgctt tgataaccgg cggttcgcct tgcagataac
    27601 gattaatatc gcgcagcaac gcctgcggcc ctttccaggt ttcaaagacg acctgggcta
    27661 tttcacgcgg cagcgggtag cagtggcgac gccagaaatc ggcgcaggcc tggtagcgta
    27721 gcagagactc atcttcaatc agctgctgct caaacagcat gccggattca aaggcattca
    27781 ggttgagcat gcgctggcaa aagccgtgaa tagtaaagac tgccgcttca tccatctgcc
    27841 gttcggctaa caacaaccac tgcgcggctt gcgctttatc gtcgatctct tccagcaggc
    27901 gttcgtacag tggattgtcg gtggtttcac gcagacaggc gatgcgcaac tcgtggatat
    27961 tgctacggat acgaccgcgc aattctgccg tggcagcctc ggtaaaggtg accaccagca
    28021 gttcttcaac gatcagcggg cggggaaagg cggcggaacc gcctagtcca agtaacaggc
    28081 gcaaatagag cgccgcaatc gtaaaggttt tgcctgtgcc ggcagaggct tcaatcaggc
    28141 gctcgccctg taagggcaag cgcaaaggat ctagtgtctc ggcgacatca ctcattcttt
    28201 tcactcatca ggggcattgt ttgctgcaac gcgctgacgt tctcccacac tttccagcct
    28261 tcaggatgca catattcggc tttcccgttc tggctgccgg aaatctgcga cagaatagcc
    28321 atgccttgcg gctcgaccac cgcctgatgg aagaaatcag caagtttttg cggcgtcagc
    28381 agttttatct gggccacgat tttatcacgc gaatcgaagc gcatattgcc gcgatcgaaa
    28441 tctttactta acttcgatgc ttcttcgccg agcgtttgcg gtgcctgcag catctgggta
    28501 attaccgcct gctggatttg cgcaaactca tctggcttca tcgctcgcaa ttttgcctct
    28561 gcggttggga aaaacgcctt gtaacgctcc cacaagaatg aaggctgttt atcattgctt
    28621 tgcaaaagga agcccatgcc ccactgacgc cccacgctca ttggaaacgc aaacacggca
    28681 tagcccaatt gttcttcggt acgcaactga ttgtagaacc acggctgtac gatctgcccc
    28741 aacagagagc tataggctga gctggtgtat tcatcgtagc cagtcggtac aaataccgct
    28801 gccagtgcgg agtcggtgct gttaccggct ttttcaaaga tgacggattg ttttttatcg
    28861 accactacat ctttgtttcg acaccactct gaaccatcag cgcccaactg tttttgcaca
    28921 tcgcgtgcca gcgttgttgc ctgggcctcg gtcatgttgc cgataaccat aaactctggt
    28981 cgagcccctg attttaaggc gtcgcgatag gccagcacct ctttcaacgt aatggagggc
    29041 aaaattttac gccgttcatc tcgcgagaag tacggcactt gcgagagcat ctgcgcgggc
    29101 ataatcgcct gctcaaacgc tttacccttt tctgcggaat ccatcatctg gttataccag
    29161 gacttcgcct gctcaagctg ctcttccgta gcggtatagc taaagtaccc ctcgagcaat
    29221 gcctggaaca gctgcggcag acgctgggtg taaccattag cattaatcat aaggccgttg
    29281 ttagcgttgg tggaaaaact tatgccacca accgacgcct ggttgcttaa ctgatcaagc
    29341 gccagccctg cgagataatc attgagcgca aacatcacct gattgcgggc gctgtccatg
    29401 gctttcggat tacgcaaaat caggctgaca tcagctttgg gctcgctggc aaaataacgg
    29461 cttggcgcat acaccacgcg cagattcgac tcatcaacaa tcagctctgg atggtcgtat
    29521 ttcttctctg acttaatcag cgagaaatca tcaggaatat aagggttaag ctctggcaaa
    29581 gagagcgcaa tgtcggcggc ttttttctgc cagtcggcga aagtttgtgc gctgatttta
    29641 tcgacctgat acggcgcatc gacaaagtaa gccgttttgt tgtgcggctc tttcgggctg
    29701 atataccaga tacgcgcatt ctgcggcgtc atcatcgcca gacgttcctt tactgcttta
    29761 gcatcgtacc gatcggcaat attgactgca tccagcgtat gctcaacagg aacgcgaatc
    29821 atggtatctg ccagccattc gacgtaatcc atatcacggg tgatcgacgg ataacggaag
    29881 tcgatatcca gcacattcgc cagttcatcg aagtattgtt tatcaatgcc tttttcacgt
    29941 aacagattga gatagctaaa aattgccgcc acaacctgat cgcgattagc caggccttta
    30001 tcagttaaag acgcagagat cgctaatacg ccgctgttgc cgttgacgat aggatcggag
    30061 ttggcgctaa tgccctcaac taatccctgc ttttgcagcc agtcagaaag tgtacctggg
    30121 ctgcgattgc caatcagata ggtaatcaat tcatcggttt tactacggaa cttcgctgag
    30181 ttgttatcga tgcgaaactc aacgcgcaac actttacgcg gcagcgcagg gacgtaatga
    30241 atgataatgc ccttttgcgc gtcggtgact accggcacgg tgatttccgg ttttttgctc
    30301 tctttgttcg gcacgcgacc aaaggtgtcc gccgccattt ttgccaactc cggcagcggt
    30361 ttattactgt aaataaccgc cttcatcaaa ttggcggaat agtacttctc gtggaaatct
    30421 ttcagcgcct gctgcaccgg attaccaggt ttgtcgctta aagtttcgag gttaccacca
    30481 gaaaactttg aaccggggtg tgccgggtta atggtttctg cgctgacctg tgccatgcgc
    30541 atcccgtcac gcgtacgcgc catggttaat tcagcgttca ctgcattacg ctcacgttcg
    30601 gcgtatttct tgtcgagcaa gggttctgca atagcatcgg ccaggcggtc taccgcaccg
    30661 ggcaaggcgt cgttctcaac ttccagatag aaagccgtgc gatacggcgc agtgctggca
    30721 ttgtgactac cgccgtgcat tttgagatat tcggccagac tgtcagcctg cgggtacttt
    30781 ttcgacccca tcagactcat atgttcaagg taatgtgcca gcccctggta cgcctcggga
    30841 tcttccagcg acccaacggg cactaccagc gccgagagcg atttaactgc ctgcggatca
    30901 gaaaccagca agaccaccat accgttatcc agacgtatag cctgatactg gcggttatct
    30961 ttatcacttt tacggatggt ttcctgaatc ggctgccatc ccgtttctgc ctgacttaat
    31021 ggtgcccaga gggcaacaaa caacaataat gctttgaacc aggtgctgcg gggcattcac
    31081 ggacctcata atcaacttta ttttctggac agattcaaca ggttaacgca aatcatctgc
    31141 cagaatttaa tcttgtgctg cacgagtcag cctatgttta tataaccatc agtccgtgac
    31201 tggtgcgcat cataaagtaa gcagatagat tgcgcaattt ttatacaaca ctcatgactg
    31261 attaaagcga aacagcggta acaggaaacg ttgcgactgt tcaacgatgg cctccattgt
    31321 ctctggtgtt aattgccgcc agagccgttg ataccagata tcatcacctt cgccacgcac
    31381 catcatgttg ccttcgtaag cctgaaggaa tttcgtacgg gctttttgca acgtggaatc
    31441 gtcatccagc atggcatcgt tttgcgcgtc ataacaggtt tttagccacg cgccgccact
    31501 ttcaggtaac accagcaatg gcgcggacat tccttcacga tacccctcaa tcagttgtga
    31561 gaggtaatgc aaagcctgtt cggctgcaag cggcggaaaa cgccactcac cgtctttgcg
    31621 tagaaaaagg cgactttcgc cattaccacc gctggcacag tagacaaggt gttccagcca
    31681 aagttgcatt ccctgcgcca cacttaataa agagggacgc cagcgcaaca ggccatccgg
    31741 ctgcacctgc ggcaaccagc cagttatctg cacaccgttg caggcgagat caatttccat
    31801 actctgcccc ggctggcgac aggcaatgac tctgtcggca agctgctgca tctcctggca
    31861 ctgtgtttcc cagaaaattt caccaaaagc gccatacggt aaatcccctg ccgcccggaa
    31921 gcggcggaac aagcgttcgg catcatcctg ctcaaccagt gcattcaata actgctgatt
    31981 gatttgataa cggctaagtc cttccagaat aaatggctcg gtgtctggga tttcgctgtc
    32041 ttcagtatgg aagttcacct gcaaacgcat ctggaagaac gcccgcaccg gatgtgccca
    32101 gaatcgttgt agcgtttcca gcggcacggt ttccggtaag gtaaacggca gcggctgaac
    32161 aaattcagaa tgtgctttac cagcctggct ggccgcaggt agccattcac gagcatagct
    32221 ttgtcgttcg cctggctggt agttttgcgg atcaaacggc atccgggtat ggaggcaagt
    32281 aagatgcgct tttacccttg cctcgctttc atcacagttg agcgcttcat cgcccggtag
    32341 ataatgactt tgcccgatgt agtcgatcag ttcctgcacc agtaccgacg ggaaacgttc
    32401 actgttatcc tgaatggaac gcccgatata gctgatatag agtttttgct gcgcggaaat
    32461 taacgcttcc aggaacaggt agcggtcgtc atcgcgacgg ctacggtcgc cacgcttcgg
    32521 tttctggctc atcaggtcaa agcccaatgg cgcaagctga cgtggataaa cgccgtcgtt
    32581 cattcccagc aggcaaacca ctttgaacgg aattgaacgc attggcatca gagtacaaat
    32641 gttaaccggt ccggcgagaa aacgctggct gatacgttct tgatccagac gctgtgccag
    32701 ttcatcacgc aatagtgaca gcggcaccgc gtcgccatac tgcgcaccta aaccttcggc
    32761 gataatcgcc tgccattgtt gttcgatcag cgtcatcgcc gcttcggttt ccgcatccgg
    32821 caggaagaag gcgttgagca tatcgcgaca aaccggcaac cactcttcta gcggacgctc
    32881 ctgtgccagc ccgcgacgcc agatgtttag ctgcattagc agtgaagcca gatgccccac
    32941 cagttctgca attaagccgc tcgattcatc ataaggtaga accgattgcc actcgccctg
    33001 cgcgctctcc atcgcgtagc ccagcaacat acgcgtcagg ccaaatcgcc aggtgtgttg
    33061 tccggtggcg gggagttcca gctcgcgaac gttgtcgtca tctatccccc aacgaatgcc
    33121 ggattcgttg acccactggc gtaaataacg cagcccttct tcggtgatgt caaaccgcgc
    33181 cgccagcacc ggcacatcca gcagcgccag cacatcctct gacacaaagc gactgtcagg
    33241 cagtgataac aggctgataa acgcttccag taccggatgt gactgccgcg cacgacggtc
    33301 ggaaatggcg taaggcaggt aacgatccgc aggtgcgcta ccaaacactg cctgaataaa
    33361 cggactgtag ctgtcgatat cagccaccat cacgatgatg tcgcgcggag taagtgtcgg
    33421 ggcttcctcc agcatcgcca gcaagcgatc gtgtaaaact tcaacttcac gctgcgggct
    33481 atggcaaacg tggaatgtga tactgctatc cagtggatca agcgggcgtt tgttatcgct
    33541 acgggaaaac tcttcgatgt tcacacccgc aacggcgcgg ttttccagtt ccagaatgtc
    33601 agactgaatg ttatgcagca ggttatctgg cgtcacatcg acaaaagcgt ccagctcctg
    33661 gctgctctcc aggtcagaaa ggagataaat gtagtcgcgc ccaagcttcc cccatgaggc
    33721 cagcagcgag ttgccgacat cctgttcacc atcgctgtta aagagctgtc cggcattttc
    33781 gctatcgcga aatagcggca attcgcgatc ttcaaaactg tgtcgacgct gacgagtcag
    33841 cagtttcgcc agataagcag gatctttaat atcgccccag taataacggc aggggttggt
    33901 aaacaggaga tggatttcaa tatgtttacc cagcgcctgg agcgcctgga gataaacagg
    33961 cggtaacgcg gaaataccgc atataaagac gcgcgaaggt aaccccggcg ggcaggtcgt
    34021 cgcggactcc agcgtttcga taaagcgctg atagagattc gcgcggtgcc agcgcggttg
    34081 tccgagttca tgggtatatt ccaccagcgc cttccacaac ggggcttgcc aggcctgtgc
    34141 ttctcccagc ccttcaacca aatgtcctgt ttcccactgt gccagccagt ccggacgata
    34201 gaccagatac tggtcaaaca ggtccgccgc ttttgaggaa agctggaaca gttttcgctt
    34261 gtcgctatcg tcagtcagat aatgccgcaa cagggtaaag tcttcgcgct ccagcaattg
    34321 cggcagcaga gtcatcagtt tccagctcat gctctgtttg ttaaaggcgc tctctttggg
    34381 gatttccggt aacacccgga cgaacatatc ccagataaag ctcgctggca gcggaaaatc
    34441 aatgtttgcc gcaataccaa acttttgcga cagggtcatt tgcagccact gcgccatacc
    34501 ggtactttgc accagaatca tctctggttc gaaaggatcg tccagccgtt cgcgttcgac
    34561 aataaactcc atcaacgctt ccagcacgtc cagacgattg gaatggtaga cccttaacat
    34621 agcggctcct gactactgac aattcgggca atgcaggcgg gtcatctcgc cttctctgcc
    34681 cccgggtgaa actagcgtaa cgctgatgct gacacatccc gcctgcgatg tctgcattcg
    34741 gttgacctgc cagttggcag gtggcgaaat cgcgcgcagt tgcgtttgct gccaaccatg
    34801 ccgccagagc ttttggtact ggtttctgct ggcgagactg ttcattgatg ttcgctggat
    34861 acccgataac gcagtgacaa tcatcaccat cagcaccatc agcaccatca gcaccatcag
    34921 caccatcgca accattacct ccggcaggct aaagccttgt tgattcttca gggaagctga
    34981 cataatgccc cctctttcag cggacaaaaa tcgctccagc cgcgtggcga aaaaacaatt
    35041 tttccatcaa tgacttcgcc tgttcgccac aacgaaacgc cttcataacc ggcaatcaat
    35101 aaggcttcat tatctgccag taaacgcaaa caaacctggg catcggttcc agcgtactgc
    35161 gagcactgaa ctgctggctg cgtctgccag gagtgcattt ttccccacgc cagcgccgac
    35221 tgaacgatgg cctggcggcg caatgactga ctttccatgc tcacgcgaga ggcaaaactg
    35281 cgatcctgct gactcattcc ttgcaatagc aagctaccca aaaccagcag catcaggacc
    35341 agagccagtg acgaaacacc tttttcgcgg ttcacaggtt gaatcctgtc acgctatagc
    35401 tggcatccac cacggtttgc ggttcagact tactggcagc acgcatatta accgtcaaca
    35461 ccggcgagaa gccgctgaca tcctgacgta cgacctgaaa agtgtcgata atgatggcat
    35521 ccggattagt gactttatcc cagcccttac cttcacagga tgtcgcaccg cgtagcgttt
    35581 ccagcacatg ctccttcaga cgaaatccaa tctggtcgga ctcttttacc ggttcgcgat
    35641 cccagatacc gttactgttc gcatcccact gcacaatgat acagtcaccc tgtccgacaa
    35701 tttccagccc ttcgccggta cagctgccat gacaataacc cgccctctgg agatgcttcg
    35761 cgacggtaaa tacccgcagc cagatttcat cttccagtgc cagcttacga gtgctcgtta
    35821 aactttcacg ctgtaacgca ggcagaaagc gtgcagcccc cagcaacaat acgctactga
    35881 tcgccatagc aatcaacact tccagcagag aaaaaccttg ctcttttaca ggcatccttc
    35941 tgtttctcct tgctgataaa gccggagtct tccccacggc gaaaccacca gccaccactc
    36001 gcccgttgag tttttgaagc gaatatgccc ggcccatgcg gtattgcgca ggccaaagaa
    36061 agcaagcgaa ggtgtcaggt cgctcatttc gacttcgggc cagcgtggca caaagaccaa
    36121 tggtgaactg ccatgacagg tattggcccc agcagcggaa ctcacaaggc accataacgt
    36181 cccctccctg ataacgctga tactgtggtc gcggttatgc cagttggcat cttcacgtaa
    36241 atagagcaaa tagtcccgcg cctagctggc ggtttgccaa agccgttgcg actgctgcca
    36301 gtattgccag ccatagagtc cacttgcgct tagcatgacc aaaatcagca tcgcgaccag
    36361 cgtttcaatc agcgtataac cacgttgtgt tttcatgccg gcagtatgga gcgaggagaa
    36421 aaaaagacga gggccagttt ctatttcttc ggcgcatctt ccggactatt tacgccgttg
    36481 caggacgttg caaaatttcg ggaaggcgtc tcgaagaatt taacggaggg taaaaaaacc
    36541 gacgcacact ggcgtcggct ctggcaggat gtttcgtaat tagatagcca ccggcgcttt
    36601 aacgcccgga tgtggatcgt agccttcaat ctcaaagtct tcgaaacggt agtcgaagat
    36661 ggattcgggt ttacgtttga taatcaactt cggcagcgga cgcggttcgc ggcttaattg
    36721 cagatgagtt tgatccatat ggttgctgta caggtgcgta tcgccaccgg tccagacaaa
    36781 atcacccact tccagatcgc actgctgcgc catcatatgc accagtaacg cgtagctggc
    36841 aatgttgaac ggcaggccga ggaagacgtc acaggagcgc tgataaagct ggcaagagag
    36901 tttgccgtct gccacataga actggaagaa tgcatggcac ggtgccagcg ccattttatc
    36961 cagttcgcct acgttccacg ctgaaacaat aatgcggcgc gaatccgggt cgtttttcag
    37021 ctggttcagt accgtagtaa tctggtcaat atgacgacca tctggcgttg gccaggcgcg
    37081 ccactgttta ccatacactg gcccgaggtc gccattttca tcggcccatt cgtcccagat
    37141 ggtgacattg ttttcgtgta gataagcaat gttagtatcg ccctgcagga accacagcag
    37201 ttcgtggatg atggaacgca ggtggcaacg tttggttgtc accagcggga atccatcctg
    37261 caggttaaaa cgcatctgat gaccaaaaat ggaaagcgtt ccggttccgg tacggtcgtt
    37321 tttctgtgtg ccttcgtcga gcactttttg catcagttct aaatactgtt tcatggttcc
    37381 tcaggaaacg tgttgctgtg ggctgcgacg atatgcccag accatcatga tcacacccgc
    37441 gacaatcatc gggatggaaa gaatttgccc catgctgatg tactgcaccc aggcaccggt
    37501 aaactgcgcg tcgggctggc ggaaaaactc aacaatgatg cgaaacgcgc cgtagccaat
    37561 caggaacaag cctgagacag ctcccattgg gcgcggttta cgaatataca ggttgaggat
    37621 aataaacagc accacacctt ccagcagcag ttcgtaaagc tgtgatgggt ggcgcggcag
    37681 cacaccgtaa gtgtcgaaaa tggattgcca ctgcgggttg gtttgcagca gcaaaatatc
    37741 ttctgtacgg gagcctggga acagcatggc aaacgggaag ttcgggtcaa cgcggcccca
    37801 caattcaccg ttaataaagt tgcccagacg cccggcacca agaccaaacg gaatgagtgg
    37861 tgcgataaaa tcagagacct ggaagaagga acgtttagta cggcgggcga agataatcat
    37921 caccacgata acgccaatca ggccgccgtg gaaagacatg ccgccgtccc agacacggaa
    37981 cagatacagc ggatcggcca taaactgcgg gaaattgtag aacagaacat aaccaatacg
    38041 tcccccgagg aagacgccga ggaagcccgc atagagtaag ttttcaactt catttttggt
    38101 ccagccactg cccggacgat tcgcccgacg tgttgccagc cacattgcaa aaatgaaccc
    38161 caccagatac atcaggccgt accagtgaag cgccacgggt cctattgaga aaatgaccgg
    38221 atcaaactcc ggaaaatgca gatagctact ggtcatctgt caccacaagt tcttgttatt
    38281 tcgctgaaag agaacagcga ttgaaatgcg cgccgcaggt ttcaggcgct ccaaaggtgc
    38341 gaataatagc acaaggggac ctggctggtt gccggatacc gttaaaagat atgtatatga
    38401 tccgcgctat aaccctccgc gaatcagccc gcccatgccg cgacgctcca taaaggctgc
    38461 aacctgatga cgaacttcgg tcgccagttg cgcttccaga ctacgctgcg caagattttc
    38521 tgcttcggca aaatcaatgc gccgcagcag gtattttacc cgcgctacag aacgtccgtt
    38581 catagacaga tggcgatacc caagcccaat gaggattgcc acgcacatgg gatcgcccgc
    38641 catttcaccg cacaaacgaa gatcgatacc atgtatttcc gcttcccggg cgatcatcgc
    38701 cagagctcgt aacattgcag gatgaagact gtcgtaaatg ttcgccaccc gggtattgtt
    38761 gcgatcaacg gccagaatgt attgagtcag atcgttggtg ccaacagaga tgaaatcgac
    38821 cctctttgcc agatgcggca gcataaatac cattgacggc acttccagca tgatgccgat
    38881 acgtggtttg ggaatttcgt aaccgatcat ctcctcgact tcacgtccgg cacgttcaat
    38941 caggcggcgt gcttcgtcaa cttcatcgag gctggtgacc atcggcaaca gaatattcag
    39001 gttgcccgta gcggcattag cacgcagcat cgcccgcacc tggatcaaga agatctccgg
    39061 ctgatcgagc gtaatgcgaa tcccacgcca acccaggcat ggattctctt cgctgatcgg
    39121 catgtaaggc agctgcttat ctgctccgac atccagcgta cgcaaggtga cgggtttatc
    39181 attgaacatt tgcagcatcc cctgatactg cgccacctgt tcttcttccg acgggaaacc
    39241 actttgcagc atgaatggga tttcagtgcg ataaagaccg atgccatcaa tacagctgcc
    39301 cagtttttct tcatgttccg ggcttaaacc agcattgagc atgactttta tacgctcacc
    39361 gctttttaac tgcgcgggta aattgacgtc atcttccgcc agacggctaa gctcaatctc
    39421 ttcactaatt agccgctgat attcttgcag cagtaccggc tccggatcga ccagcaattc
    39481 accgcgatag ccatcaacga tcagcgtcct acgatgcagc accgaaggct gaatatccgc
    39541 gcccatcacg gtagggatcc ccagcgcacg taccatgatc gcagcatggg agttggctgc
    39601 gccatctcgc acgacaacac cgactaagcg atcctggggc agctcagcaa gagttgtcgc
    39661 tgacagttca tctgccacca gaatgaaacg ttccggccag gcgttcggcc cttgattagc
    39721 gtcatcaaga tgaaacagca atcgctgacc cagcgcacgt aaatcaccag cccgctcttt
    39781 gagatagtta tcgcttagcg cggcaaactg ttcggcaaat ttttcaatga ccgtttttac
    39841 cgcccactct gccaccgagc ctttatcaac ctcggcaaac aattcgcgac gcagccgggt
    39901 gtccgaaagc aggtgcgagt aaagatcgaa aatagccgcc gtttcttttt gtgctccggc
    39961 ggcaaagcgt ttgctgtagc ggcgaaactc gtttgccgct tcttccagcg ccccggtcag
    40021 tcgttcgcgt tccagagccg gatccagcgt tgatgcctga tacacctgtt ccattaaagg
    40081 taacgtggca tcctgccagc cttcggcaat cgccacacca ggtgctgccg gtaatgcgcg
    40141 gattcgcgtc tggcgatatt gcccaaacag ggcagtcaac tgcgactgag aaagaatagc
    40201 tgccatctgg gtggcaagcg tcaccaggaa ggattcttca ctttcgtcat actggcgcaa
    40261 ctctcgttgc tgtaccacca gtacaccaag caactggcga cgttgaataa ttggtacgcc
    40321 taaaaacgcg cggaaacgtt cttcttttac ggaggggatg tatttgaagc tggggtgctt
    40381 ttgcgcatct gcaaggttta tcggttccgc cagcctgcca accaggccga cgatcccttc
    40441 atcaaacgcg agcgttacag tgcgaccgcg tggttttttc agcccccggg tcgccatcag
    40501 gtagtaacaa cgtcgatcat gatcggccag gtagaccgaa cagacctcgg tatccatcgc
    40561 aagacagatg tcggtaacca gaatatttaa cgcctcattc aggcgtggtg cgctggctac
    40621 cttttcgact atttcgcgca ggcgagtgag cataattggc gtgacttaac ctcttttacg
    40681 tcgataagca gatgcgtttt gtggttttgg cgtattttcc tgcagtgaca tcaccacact
    40741 cgcgaactct ttcattaccc tacggtagac atcacgttta aatgacacca cctgtctgac
    40801 cggataccag taacttaccc atcgccagcc gtcaaactct ggtgtactgc tggtttgcat
    40861 attgatttct gcatcgccgc ttaccagctg caagagaaac catttttgtt tttggccgat
    40921 acaaaccggc ttcgtgtccc aacgcaccaa acgtttcggt aatttgtagc gcaaccagtt
    40981 acgcgttgaa gcaaggattc gaacgtcttt gcggcttaat cctacttctt caaacaattc
    41041 acggtacatc gcctgctctg cggattctcc ggggttgatt ccgccttgcg gaaattgcca
    41101 ggagtgctga ccaaatcgcc gggcccacat tacctgcccc tggcgattac aaatcacgat
    41161 acctacgttt gggcggtagc catcgtcatc aatcaccgga ctacctcaaa ataaagcttt
    41221 atatacgaat gattgtttca tactccagga agacggtaaa ccactctctg cagggcatta
    41281 cacactaata acaattgaat aactcacagt tatgtgcaga gttataaaca gaggaagggg
    41341 tggatagccg tttttattca ccttttctgt ggatagagtt gtgaagaact acggaattac
    41401 tacgggaaaa cccggagcat tctgaataag cccgatatag acattttaaa atattcatta
    41461 ttagtcaatg cattacatgt aatttgagta atgaaaaaga gtaattcgtg acccaggtca
    41521 cacctctcat ttacgggttg gcgaaagatc aaccaatgcc gtgtttatcc acagaatgtg
    41581 ccactaagtt aagcactgaa ccactaaaaa ctggagtttc gtcgcacgtc aaggctgtaa
    41641 atgaaaacag tagtggaggt ttttcacagt tatcccagct ttctgtggat aacatggtgt
    41701 aagatcctgt ttattttcag tgaccagatt tggaaaacct attgcagtgt tgcgcaactc
    41761 gattaccggc agcctaaaaa gcaatataaa tcagagaatt gaacaacaca tgtggaaaaa
    41821 gttacactgc gaatattcgg cacataattg ctgtttgttt tttaaccaag gtatcatgac
    41881 atgtcccaac ctcgcccact gctctctcct cccgaaactg aagaacagtt gttagcgcaa
    41941 gcacagcaac tttctggtta tacattggga gaactggcgg cacttgacgg gctggttacg
    42001 ccagagaatt taaaacgcga taagggctgg attggcgtgt tactggagat ctggctaggt
    42061 gccagcgcag ggagtaaacc tgagcaagat tttgctgctc tgggcgtgga acttaaaact
    42121 atccctgtgg atagtcttgg tcgtccgctg gaaacaacat tcgtttgtgt tgcccggtta
    42181 acgggtaata gcggggtgac ctgggaaacc agccatgtgc gccacaagct caaacgcgta
    42241 ctgtggatac cggttgaagg cgagcgcagc atcccgctgg cgcagcgtcg cgtaggatca
    42301 ccgttgctgt ggagcccgaa tgaagaggaa gaccgacagc tacgcgaaga ctgggaagaa
    42361 ttaatggata tgattgttct cggtcaggtt gagcggatca ccgctcgtca cggggaatat
    42421 ttacagataa gaccgaaagc agcgaatgcg aaagcgctta ccgaagccat tggtgcccgg
    42481 ggcgaacgga ttctgacgct gccacgcggc ttttatttga agaagaattt caccagtgca
    42541 ctactggccc gtcattttct gatccagtag ccatcgcttt gacctgccgc tttccgggca
    42601 tataattacc gcttcatttt tttggcaggg ctttttagat gttatttgca tggataaccg
    42661 atcctaacgc ttggcttgcg ctcggtacgc tgacgctgct ggagatcgtt cttgggatcg
    42721 acaatattat tttcctttct ctggtggtgg caaagcttcc cacagcacaa cgtgctcatg
    42781 cgcgccgtct ggggttggcg ggagccatgg ttatgcgtct ggcgctgctg gcatcaatct
    42841 cttgggttac gcgcctgaca aatccgcttt ttacaatatt cagtcaggaa atttctgccc
    42901 gtgatttgat tctgcttctg ggtggcttgt tccttatctg gaaagccagc aaggaaatcc
    42961 acgaatccat tgaaggtgaa gaagaagggc tgaaaacacg cgtttcatca ttcctcggcg
    43021 ctatcgtgca gattatgctg ctggatatta tcttcagcct cgactcggtg attaccgctg
    43081 tgggtctgtc agatcacctg tttattatga tggcagccgt ggtgattgcc gtaggcgtga
    43141 tgatgttcgc cgcgcgctcg attggtgatt ttgtcgaacg ccatccttcg gtaaaaatgc
    43201 tggcgctctc tttcctgatt ctggtgggct ttaccctgat tctggaaagt ttcgacatcc
    43261 acgtaccgaa aggttacatc tacttcgcga tgttcttctc tattgcggtt gaaagcctca
    43321 acttgattcg caacaaaaag aatccgctct gatactccgt acgctctcct gcgggagggc
    43381 gtactcactc cttttaccct cacagtttaa gattttcctg ctttcaagat atataacgtc
    43441 ggtttataaa cagactatta tcataggtag gccaaatgag gataaccgga tgaaaaaatg
    43501 ggcagtaata atttctgcag tcggactggc gtttgctgtt tccgggtgtt ccagtgatta
    43561 cgtcatggcg accaaagatg gccgtatgat tttgaccgat ggaaaacctg aaattgatga
    43621 tgataccggg ctggtgagtt atcacgatca gcaaggtaac gcgatgcaaa ttaaccgtga
    43681 tgatgtttcg caaattattg aacgttaaca aataaggtca gcatccggct ggccttaaga
    43741 tttttctctt tcccttttcc cttccctctg ccatttttat attccttatg tcgtgattat
    43801 aaaaaggaaa cggctatgca atatcaccgt ataccccaca gttcgctgga agtcagcacg
    43861 ctggggcttg gcacgatgac gtttggtgaa cagaacagcg aagccgacgc ccacgcacaa
    43921 ctcgactatg ccgtcgctca gggcattaac cttatcgacg ttgccgaaat gtacccagta
    43981 cctccgcgcc ccgaaacgca agggttaacc gaaacctacg tcggcaactg gctggcgaaa
    44041 catggcagcc gcgaaaagtt aattatcgcc tccaaagtga gcggaccgtc gcgcaataat
    44101 gacaagggca tccgcccgga tcaggcgctg gatcggaaga atatccgcga agcgctgcat
    44161 gacagcctca agcgcctaca gactgattac ctcgatcttt atcaggtgca ctggccgcag
    44221 cgcccgacca actgcttcgg caaactcggt tatagctgga cggattctgc gcctgcggtt
    44281 tcgctgctgg atacgctgga cgcactggca gagtaccaac gcgcgggaaa aattcgttat
    44341 atcggcgtgt cgaacgaaac tgcatttggc gtaatgcgct acctgcatct ggcggacaaa
    44401 cacgatctgc cgcgtattgt caccattcag aacccttaca gtctgttaaa ccgcagtttt
    44461 gaagtaggtc tggcagaagt cagccagtat gaaggggtcg aactgctggc ctattcgtgc
    44521 ctgggtttcg gcacgctgac cgggaaatat ctcaatggtg caaaacccgc tggcgcacgt
    44581 aatacgctct ttagtcggtt cacccgctat agcggtgagc aaacgcaaaa agccgtcgcg
    44641 gcgtatgttg atatcgccag acgtcatggc cttgatcctg ctcagatggc gctcgcgttt
    44701 gtacgccgtc aaccgtttgt tgccagcact ctgctgggcg caaccacgat ggatcagctg
    44761 aaaactaaca tcgaaagttt gcatctggag ttaagcgaag acgtattagc tgaaattgaa
    44821 gcggtacatc aggtttatac ttatcctgca ccataaagat taacgccggt taaaaccggc
    44881 gttaaatatt aatgacggcg ctgccagatc cacagcgccg ttattgccag cgcaaatagc
    44941 gcaccgaagc caatgccaat gggcacgacc gggatgccta ccattaccgc cagcgagtaa
    45001 atgcccagca tcaacaacat ggcgctgttt tcgccaaggt tttgtactgc aatcgcattc
    45061 cccgccccga cgcttttttt accccgctcc tgtagcaacg cattgagcgg aacgacaaaa
    45121 aagcccccca gcacgccaat cagcatcaac aaggcatagg ctggcagcag ctcgtgttgc
    45181 agggagaaaa tcggtaccac cacgccaatc aaaatcccgg ctggcataca gcgtgacacg
    45241 gtttccagcg taaccagctt cgctgccgca cctgcgccaa ccacgatacc aatcgctacc
    45301 atcgcgttga gataggtcgg cgtagcgtta tcggtaatgc ccagcgccac cggtacccac
    45361 agcaccaaca ggaaacgcag cgtgacaccc gctccccaga ataaactggt gcccaccagc
    45421 gaaaaacgcg tttcaccatt gcaccatagc gaggtgcagg cattcaggaa actgcgggtc
    45481 atgttgatga gattccagga ctgccccgga cgcgccgcca gtttgggaat gtagatattg
    45541 gcaacgaccg caccaccgta ggccagtgcg catgcggcca gagcgaccag gacatgccag
    45601 tcagccagca caccaccggc tacggaaccg agtaaaatcg ccgctatggc agaagcttcc
    45661 attaaaccgt tagcttttac taacttacta cccgtggtta attcgccgag aataccgtat
    45721 ttcgccggtg aataggctgc agcaccaaca cccaccagcg tatagccgag aaacggattg
    45781 ataccaaagc agatactggc tgcgcccagc agcttcaggc cgttggcaaa catcatcacc
    45841 cggcctttgg cgaagctatc cgccacctgc ccgacaaacg gcgccaaaag aatgtaagca
    45901 cctacaaaca ccatttgcag gatgggctgg ctccactccg gatagaactg cgctttcagt
    45961 aacgccagag tggcaaacag tagcgcgtta tcgccaaacg cagagagaaa ctgcgccacg
    46021 ataaccgctt tcatcccctt cgaccacaac gaagtgttag tgtgcactga ctcactcatc
    46081 gtgttgttcc gcttcgtcta cccagctttt caacgtgaca aagtcaggtt tgccgctgcc
    46141 aagtaatggc atctgtttca gatagcgaat atcgcgcggt acagcaagct ccggcacgcc
    46201 gtgctcgcgg gcatactgtt gcaacttatc gcgcgtcagt tcgttatctg tggtgaaaag
    46261 caccagtgcc tcgcctttgc tggcatcgct cttaatcgca gtggcatgga ctttatctgg
    46321 cgaaacacca agtgccagtt gttccaccat ttccagcgac accatttcgc ctgcaatttt
    46381 ggcaaagcgt tttgcgcggc cctgaatctg cacaaagccc tgctcgtcaa aacgcacaat
    46441 atcgccagtg tcataccagc cgcgctccat ttcgccgcga acattctcgg cggtgggcac
    46501 ttccagtaca cctggcttct ccacccgcag atagccgttc attatgttcg gccctttcag
    46561 ttgcaggcgt ccgccctctt cgataccagg gaccgacaac aggcgtgcat ccattcctgg
    46621 tagaatacgc cctaccgtac cgggtttcgc cgccatcggt acgttgatag aaacgacagg
    46681 cgcgcattcg gtcacaccgt agccttcaag gatgcgcagg ccaaatttat cctgccaaag
    46741 ctgtttggta ctttcttgta gtttttctgc gcctgccacc acatagcgta gacgatagaa
    46801 gtcatacggg ttggcgaaac gcgcgtagtg accgaggaaa gtcgaggtac cgaacaacac
    46861 ggtgcaactg cagtcataca ccaagtccgg cacaatgcgg taatgcagcg ggcttggata
    46921 aaggaacact tctgcacctg taagcagtgg cgtaaacagg cctaccgtca gcccaaagga
    46981 gtgaaacagc ggtaatgccg acataaagcg atcgttggtg gtgaagtcgg caatcgtttt
    47041 aatctgctcg acgttcgcca gaatgctttt atggctatgg acgacgcctt ttggatggcc
    47101 ttcagaaccg gaggtaaaga ggatcagcgc ctcttcttcc ggctgctgtt ttacctgcgc
    47161 caggcgcggc atcagcaaat gagcgaagat ccatactttg tcggcagtgg tgacatctgc
    47221 ttttaaatct tccagataga cccagcgcac ctgagtaagt tgctccggca gatgccagag
    47281 tttgccttta tcgagaaact ggcgggaagt gaagatggtt ttgatttcag ccgccgtaat
    47341 agcactggtc agccctttta ccccggcagt gtagttcatc attgcgggaa tgcggcgacg
    47401 cgcgatggcc ccaaaaatca ctgccgcact gatgcctgca ttgggcagca ttaagccgat
    47461 gcgttcgcct tcaacactgt atttttcaag gatgcgtcca acaaacagcg ttttcgtaag
    47521 caatttgcga taggagtctg gggtaaagtt gacgtcttcg acacatttct tcccggctcc
    47581 gaagcggtac attgcactca gtaaagattc gtacagagtt tcacgcgggc gcaccgccat
    47641 acgcgcttcc atcattattt gatgcagcat ttcgccagcg attttgcgac ggtcacgggc
    47701 acgcggcgca tccggcatcg ccacctgcgt tggaggcaaa atatgcagag taatttgcgg
    47761 gaacaagcga cgtttaacca gacctttcag gcggctgaag tgcgtaagtt ccgccccttc
    47821 aatacgcaca ggaataaccg ttgcaccaga cttcgccgcg acaaaacccg cgccatcgta
    47881 gattttcatc agcgagcctg tagtggtgat gcgtccttca gggaaaatca ccactggtcg
    47941 gccctgttcc accagacgta ccagatgttt aatagccata ggttgcgtcg ggtcgagagg
    48001 aacaaagtcg ataaatgatt tcagccaacg catataccac tgttggctta ttgaggtgta
    48061 aacggcaaac actggacgca caggtaaaaa cagtccaagc aaaatgccat caataaaaga
    48121 gacgtgatta ggcgtaatta gtacgcgctc gcccttcagt gcctgggtgt cacccgtaac
    48181 gcgaacgcga tacaaaacac ggcacaaatt tcgaaaaaag ctaaaaagca ttccaactcc
    48241 ctttgctctg attcagtaaa agcgaatgga gggagattac acgagataaa gaacgcgagc
    48301 gacagtaaat taggtgcgaa aaaaaacctg cgcatccgcg caggttggtg caagagacag
    48361 ggtacgaaga gcgtaccgaa taatctcacc aatcaatacc tctgggatct tgattgtggt
    48421 ctgcacgacg actcttcgcc agcgagaaaa cgcaaaggaa tgaagggaaa tgcaacgagg
    48481 tgtgtaaatt gtcggttact gttacagatt gatgaccggc aaaaaaaaac ctgcgcatct
    48541 gcgcaggctg gtgtaattca tgtgctcaac acgaagttga cttcacctat caatacctct
    48601 gggatcacca ctttagcaac ctgaagccaa acgccaccag cggtcaatcg caacagcgtt
    48661 tcgcaaagtg taaccaaagg tttgcaatct ctttttttgt cttacttatt tgcgtttggc
    48721 tcacattccc acgatgaaaa cacgccaccc cttgaaccaa cgggcgtttt ccgtaacact
    48781 gaaacaatgt aagcgtttac cctctaaggt attttcatgg cgaccataaa ggatgtagcc
    48841 cgactggcag gcgtttcagt cgccaccgtt tcccgcgtca ttaataattc acccaaagcc
    48901 agcgaagctt cccggctggc tgtgcatagt gcaatggagt ctcttagcta tcacccgaac
    48961 gccaacgccc gtgcgctggc gcagcagacc actgaaacgg tcggtctgat cgttggtgat
    49021 gtttccgatc cgtttttcgg tgcaatggtg aaagcggtcg aacaggtggc ttatcacacc
    49081 ggtaatttct tattgattgg caacggttac cacaacgaac aaaaagagcg tcaggctatt
    49141 gagcaactga tccgccatcg ctgtgctgcg ttggtcgtcc atgccaaaat gatcccagat
    49201 gccgatttag cctcattaat gaaacaaatg cccggtatgg tgctgatcaa ccgtatcctg
    49261 cctggctttg aaaaccgttg tattgctctt gacgatcgtt acggtgcctg gctggcaacg
    49321 cgtcatttaa ttcagcaagg tcatacccgc attggttatc tgtgctctaa ccactctatt
    49381 tctgacgccg aagatcgtct gcaagggtat tacgatgccc ttgctgaaag tggtattccg
    49441 gccaatgatc ggctggtgac atttggcgaa ccagacgaaa gcggcggcga acaggcaatg
    49501 accgagcttt tgggacgagg aagaaatttc actgcggtag cctgttataa cgattcaatg
    49561 gcggcgggcg cgatgggcgt tctcaatgat aatggtattg atgtaccggg tgagatttcg
    49621 ttaattggct ttgatgatgt gctggtgtca cgctatgtgc gtccgcgcct gaccaccgtg
    49681 cgttacccaa tcgtaacgat ggcgacccag gctgccgaac tggctttggc actggcggat
    49741 aatcgccctc tcccggaaat tactaatgta tttagtccga cgctggtacg tcgtcattca
    49801 gtgtcaactc cgtcgctgga ggcaagtcat catgcaacca gcgactaacc gcagttaaag
    49861 caattccagc gccagtaatt cttcgatggt ctggcgacgg cgaatcaacc gcgcctgacc
    49921 attatcaaac agaacttctg gtaacagcgg acggctattg tagttggatg acattgatgc
    49981 gccatatgcc cctgtatcat gcagtaccag ataatcacct gccttcactt ccggcaaggc
    50041 gcgggtttca acatttcccc cttcctgctg ggtaaagaca tcgcccgatt cacataacgg
    50101 cccggcgacg acggtttcca ccgttggcgc gtgttccaga gaacgaccat cagctgccag
    50161 ggcactgata tggtggtaac taccgtacat tgccgggcgc atcagatcgt tgaacccggc
    50221 atcaaccagc acaaagtggc ggctacccat ttgtttgacg ctccgcacct gagtaattaa
    50281 tacgccagac tgcgctacca ggaagcgacc cggttcaatt tccagtttca cagggtggcc
    50341 caaatggcga gcgatttgct cacgcgcggc attccacaga ccataataat gttcggtatc
    50401 aaccgcctct tcaccctgtt gataaggaat agaaagccca ccgcccgcag aaatagcctg
    50461 taaatcctga ccgaattcga taacctgacg caccatagca ccacacacct gctccagatg
    50521 ggcataatca acgccagaac caatgtgcat gtgaatgccg actagctgca gatgatgacg
    50581 ttgtatcacg tccagtgcgg cgggcagatc ggtgtaccag ataccgtgct tgctgttttc
    50641 gccaccggta ttggtttttt ggctatgtcc gtgaccaaac cccggattaa cgcgcagcca
    50701 tacccgatgc cctggcgaaa cctggcccag ttggtcgagc gtatcaacag aacccgcatt
    50761 caccggaatt tgcaattcac tgacgcgttc aagcgtcgcc tgatcgataa catctgccgt
    50821 aaaaacaata tcatcggggt gcgtttgcgg attgtaaccc gccgccaacg cacgctctat
    50881 ttcgcctaac gagacggaat ccactttcac gccctgctca cgcattaagc gcaaaatatg
    50941 aatattggaa caggctttct gtgcaaagcg caccacttca aactgtttca gcgctgcaat
    51001 ctgccgacga ataatttgcg catcgtagac ccacaccggg cagccaaatt cagcgggcaa
    51061 acgcagcaga ttttcggcgg tgagatcggt atcggtgctg aacagtgaat gtggcataac
    51121 aaactccaga tgagtgcttt tttatgatta cgccacatca taaaaagaat aaaaaatatc
    51181 gatttatgtc gagtctatgc aaaaatgata tggattaccg gattgcgaga gagcgctaat
    51241 ggccgccgtt aacttacgtc atattgaaat ttttcatgcg gtaatgaccg ccggaagcct
    51301 gactgaggca gcacacctgc tacacacctc acagccaacc gtcagccgcg aactggcgcg
    51361 ctttgagaag gtgatcgggc tgaaattgtt tgagcgcgta cgtggacgat tacatcctac
    51421 cgtgcaagga ctgcgtctgt ttgaagaagt gcaacgatcc tggtacggac tggatcgcat
    51481 tgtcagcgcc gcagaaagtc tgcgcgagtt tcgccaggga gaactgtcta ttgcctgcct
    51541 gccggtcttt tcgcaatctt ttttaccgca gctcctgcaa ccctttctgg cacgttatcc
    51601 cgatgtcagc ttaaatatcg tgccccagga atcaccgcta cttgaagagt ggctctcggc
    51661 ccagcgtcat gatttaggac tcactgagac actccatacg cctacgggaa cagaacgtac
    51721 cgaattactc tctttagatg aagtgtgtgt gttacctccg ggtcatccgc tggcggtaaa
    51781 aaaggtatta acgccggatg attttcaggg tgagaattac atcagccttt cccgtactga
    51841 cagctatcgc cagttgctgg atcagctatt tactgaacat caggttaaac gacgcatgat
    51901 cgtagaaacc cacagcgccg cgtcagtctg cgcaatggta cgggcggggg taggtatttc
    51961 ggtggttaac ccgctcaccg cactagatta tgcggcaagc ggtttagtgg tgcggcggtt
    52021 cagtattgcg gttccgttca ccgtcagcct gatccgcccc ctgcaccgcc cgtcatcagc
    52081 gctggttcag gcgtttagtg ggcatttaca agcggggtta ccgaaactgg tcacttctct
    52141 tgacgctatt ttgtcgtcag ctacgacagc ataaaagcga cagcatcctc ggcatggatc
    52201 gccgcggtat caaacacagg caaaacactg cgctcttctg gcaccagtaa accaatttct
    52261 gtgcagccaa aaatgacgcc ctgtgcgccc tgttctgcaa ggcgagcaat cacttgcgca
    52321 taataagcgc gtgacgcttc ggtaaattgc cccagacaca gttcttcaaa aataatctga
    52381 ttaattttcg cccgttcatc cgcttcagga ataagacaat tgatggaaaa ttgttccgtc
    52441 agccgcccgc gataaaaatc ctgttccatg gtgtaacgcg tacccagcag cgccacacga
    52501 gtcattccgg ccccggtaat tgcacgtccg gtggcatccg caatgtgtaa gaaaggcaga
    52561 gtgcaacgtg actcaatggc atccgccact ttatgcatcg tattggtaca taacacaata
    52621 ccttctgcgc ccgcccgctg taagccaagc gccgcctcag ccagaatgtc cccggtttta
    52681 tcccattccc cgcgacgctg gcactcttct atttcatgaa aatcgacgct atgtagcagc
    52741 atttgcgcag agtgaagccc accaagccgc tgtttaatgc cttcatttat cagacgatag
    52801 taaggaatgg tggattccca gctcattcct cccagcaaac caattgtttt cattcctctc
    52861 tcctgatgtg tgttaccgca acaaggctga ataaggaacg agacaaacgc ctcaacggcc
    52921 aggtgcccaa tctctattaa cgaaaaaagg gccggatgta cagcacatcc ggcccgtgaa
    52981 atcagacgcc gatatttctc aacttctcgc ctgccatcag tttgcgttcg atatgttcca
    53041 gcgtgacatt tttggtttcc ggaatgagcc agaaagtaat acccacaaac gcaatgttca
    53101 gcgcagtgta gagccagaac gtaccggcag cgccaatgct atcaagcagt gtcaggaagg
    53161 ccgcgccgat aatcatattc gacacccagt ttgtcgtcgt cgaacaggta ataccgaaat
    53221 cgcggcattt cagcggctga atttcagagc acaggatcca caccactggc gcggcgctca
    53281 tcgcataacc ggcaatacac atcatcgtca tgccaacaga gagccaggac aagccactgg
    53341 aagccgtacc gttatcaaac tgcatcaggc aatagcccag caccagagtg cctaacgcca
    53401 tcacgctgaa accaattttc agagccggtt tacgacctgc tttatctacc gtaaagaccg
    53461 caatgaaggt cgcgaacata aaggtcagtc ccacgaccag agtcgcaatc atctgttgtt
    53521 ctgtggtcgt aaagcccgcc attttgaaga tacgcggcgc gtagtacatg atgatgttca
    53581 taccggtaaa ctgctgcatc gcctgcaaca acataccgag aaacacagca cgacggacgt
    53641 tacggttgat cttaaacagt gcccaaccgc cctgttttaa cttcaggctt tcacgaattt
    53701 cgttgagttc ttctcgcgct ttttccgacg tatcgcgcag catacgcaac acttcttccg
    53761 cctcaatatg acgccccttt tccgccagcc agcgcgggct atttggcagg aagaccacca
    53821 gaataatcag cagaactgct ggtaaagcaa gaacccccaa cattgcgcgc cagttaccgc
    53881 tataactgaa cgctgtatcg gataaaaacg ccagcacgat gccgagtgtg accatcaact
    53941 ggtacatact gatcatctta ccgcgaacgt tttcacttgc catttcagaa agatacagag
    54001 gagcggtgta agacgcgatc ccgacagcaa tgcccagcac cacacgagcg gcgattaaca
    54061 tctctacgct ggtcgcaaaa gcggacccta tagaaccgag tacaaacagg atggcccccg
    54121 ccatcaggct gtatttacgc cccaggcgga acgacagcca accattaaac agcgcaccaa
    54181 ttgctgcgcc gagcatcatg ctactaacca cccattcctg caaacgactg gtcagcacaa
    54241 agtgatcggt aatgaacggc aacgctccgg cgattacgcc gatatcaaga ccaaataaca
    54301 atcctgcgac cgcagcagct accgaaacaa acatattcat acgccgcgta tcacgcaaag
    54361 aacgtggcgt taaagcagat tccgtattga tagtaaccat tttttcctgc cagcagagag
    54421 taagacattg tgaaaaaata cgtgaacaat tcacacaggt gtcaggtcgg aaacagcata
    54481 aatatggatt aaattgctgc gacatgccgt tatgtgatgg atattccaat tttaaaatta
    54541 agttgaatta ttgagattat tattaacctc ctaattttaa agcagataaa attcataaag
    54601 ttcattaatt gataattgat atggattatt tcataaccat gatatggatt atgatgatct
    54661 agagatataa aaaaccctgc catgcggcag ggtcataaaa gtaagaggaa tgaattaacg
    54721 cgccagccaa ccgccatcca cagcaatggt ataaccattt acataatctg aagcgctgga
    54781 ggcaagaaac actaccggcc ccatcaggtc actcggcagt ccccaacggc cagctggaat
    54841 gcgatcgaga atttccgcgc tacgttgttc atctgcccgc agttgttggg tattgttggt
    54901 cgccatgtaa cccggagcta tcgcattaac attaatgttg tgtttagccc attcgtttgc
    54961 catcaatcgc gtcacaccca tcacgccgct ttttgatgcg gtataagaag gcacacggat
    55021 cccgccctgg aaggagagca ttgacgcgat attgataatc ttgccgccat tgccttgcgc
    55081 gataaagtgt ttcgccgctg cctgagacat gaagaatacg ctcttgatat tcaggttcat
    55141 gacatcgtcc cagtcttttt cgctgaactc gagagcgtct tcgcggcgaa tcaatccggc
    55201 gttattcacc aggatatcaa tatgaccaaa ttcagctacc gcgcgatcca gcagtccagg
    55261 aatgccatca atctttcgca gatcggcggt caggcttaaa aaacgacgcc ccagcgccgt
    55321 gacctgctcg atggtttcag tcggttcaac gatgttaatg ccaacaatgt cacagcccgc
    55381 ttgcgccagc cccaacgcca tcccctggcc cagcccagta tcacaaccag tgacgaccgc
    55441 aactttacct tggagagaaa atgcacttaa aatcataaca acaccttatt cgtttatgcc
    55501 cacaactagc gcaaatcttt aacgcccaca tggtccatat cattaaagac ctggttttca
    55561 ccgaccatgc cccagataaa ggtataagct ttggttccga cgccggaatg gatcgaccag
    55621 ctcggggaga tcaccgcctg ctcgttatgc atcacaatgt gacgcgtttc ttgcggctgc
    55681 cccatcatgt ggaaaacgca ggcgtcatca tccatattga aatagaaata aacttccatc
    55741 cggcgctcgt gggtgtgaca cggcatggtg ttccacaagt tacctggagc cagctccgtc
    55801 agccccatac tcaattgaca ggtttccagt acatccggga caaaatattt gttaatcgtg
    55861 cgacggttac tggtgaggtt atcgcctaac gtgactggag atacttcgtc cggtgtgact
    55921 tttttggtgg gatacgtcgt atgcgcgggt gcgcaattgt aataaaactt cgccggagtg
    55981 gcggtatcaa tactggcaaa gacaacttct tttgcacctt taccaacata cagggcgtcg
    56041 cagtgaccga tttcatagca ttgaccatcg acagtaatcg taccggcacc gccaatattg
    56101 ataacaccta actcgcgacg ttcaaggaaa tagcttacgc cgagttgttt accaacttcc
    56161 ccgccaacgg aaaccgtttt agttatcggc ataatgccgc caacaataat tcggtcaatg
    56221 tggctgtaaa ccatggtgta ctcatcggcg acaaatacct tttcaaccaa aaattcattg
    56281 cgcagccctt gggtatccag cgtttttgcg tgcgcaccgt ggatgctctg tcttacgtcc
    56341 acatcaacct ccgataatca gtgaagcgtg aaaatagaaa acgatagtga aacactgttt
    56401 cgttttctct ataaagggat gatatgccca tgggctaggg ttttcaatta aaaataaaac
    56461 aatgttttat ttttaattta aggattaaaa aaagtgcagt gtcgatcacg aatatgtcat
    56521 gagaatggaa aaatgaggga gcccagaaaa tgtatgtcga cttgccagcc atcattattc
    56581 aacagattaa tgacggcaga tatttatcgg taaggaggaa atgcagagct actcgtcacg
    56641 ttcaatggtc aatgccacgc cctggccccc gccgatacaa agcgttgcca gccctttgcg
    56701 ggcattacgt ttcaccattt catgaaccag agaaaccagg attcggcaac cggaagcgcc
    56761 tatcgggtga ccgagtgcga tcgcgccacc attgacattg acccgacgct catcccactc
    56821 aagcatcttg ccaaccgaaa gcgcctgtgc agcaaacgct tcattagcct cgataagatc
    56881 gacatcagcc aactgccagc ctacacgctc caggcaacgg cgggtcgcat acaccggcgc
    56941 aattcccatc aatgccggat ctacaccaac gctggcaaat gcgcggatgc gggccagcac
    57001 gggtaaattc aacgctcgtg ctttggcttc gctcatcatc attaccgcag ctgcgccatc
    57061 gtttatggat gatgcattcc ccgcggtcac agaaccgaga ctatcaaatg aaggatttaa
    57121 acgggctaaa ccttctgcac tggcgtcagt gcgtggctgt tcatcggtat caacaaccaa
    57181 cgtctgaccg ttactttgga tcattaccgg gacgatctca tctttaaatc gtccggcgtc
    57241 aatcgccgct cgcgcttttt gttgcgagct aagtgcgtaa gcatcctgca actgacggct
    57301 gatgccatat tcgcgagcca gattttcggc ggtgacacca atatgataat cattgaaggc
    57361 atcccacaac ccatcatgca ccagactgtc aaccaactgg ctattgccaa gctgttcacc
    57421 ggtgcggcta tcagtcagaa catgtggtgc gcggctcatg ttttcctggc cacctgcgat
    57481 gacaatatca gcctcgccac actgtatcgc ctgagtagcc agatgcagtg ctttaagacc
    57541 ggaaccgcaa acgtcattaa tcgtgattgc agaaacgctg ttaggaagac cacctttaat
    57601 agccgattgc cttgccggat tctgccctgc ccctgcagtc aacacctgac caagaattac
    57661 ttcatccacc gcatatgcag gaacgccggt acgttctatt aacgctttca cgaccagact
    57721 accaagttcc acggcggaat gacccgctaa cgcaccacga aagcagccga taggtgtccg
    57781 taacgccccg acaatcacaa cgtctttcat cacaacctcg caccaattaa cagtgcaata
    57841 gtaaatgatt gttaattaca gttatcttaa ttgtttaaaa aaagtgattt ttatcacaaa
    57901 ggaaatatgc ctgagcagta gtcagagtca taactggcac gtaaggtttg caaccactaa
    57961 cccaccaata gatgggtaga tagggcgtta atctcccata cttaacttgg tttatggtaa
    58021 attgccctcc attttgttta atttgtagat gatacgttca gataatgtct aatatttggt
    58081 ctaaagaaga aactctgtga agtttcgcgc tctatggcac agccgttgat gcaggcacgc
    58141 tattccttcc tattcagtta ggttcggcag gggctgtggt cctgtttatt actgctctgg
    58201 tcgcctggcc tttaacatat tggccacata aagccttatg ccagttcatc ctctcatcaa
    58261 aaacatcagc aggtgaaggg ataacgggcg cggtaacaca ctactatggc aagaagattg
    58321 gtaatctgat taccacgctg tacttcatcg ccttttttgt cgtcgtgttg atatatgcag
    58381 tggcaattac caactcactt acggaacagc tggcaaagca tatggttatt gatcttcgca
    58441 tccgtatgtt ggtgagtctg ggtgttgtat taattctgaa tctcattttt ctgatgggac
    58501 gtcatgccac tattcgggta atgggatttt tggtattccc attgattgcc tatttcttat
    58561 ttctttccat ttaccttgtc ggtagttggc aacctgatct attaacaacc caggtagagt
    58621 tcaatcagaa tacccttcac cagatatgga tatcgattcc cgtgatggtt ttcgccttta
    58681 gccatacgcc cattatttct acgtttgcca tagacagacg tgaaaaatat ggcgaacacg
    58741 ctatggataa atgcaaaaaa attatgaaag tcgcttatct catcatctgc ataagtgtac
    58801 tgttctttgt ctttagctgc ctgctttcta ttccatcttc gtatattgaa gcggctaaag
    58861 aagaaggggt caccatttta tcggcgcttt ctatgctgcc gaacgcccca gcatggttgt
    58921 caatttccgg gattattgtc gcagtagttg cgatgtcgaa atcattcctg ggtacgtact
    58981 ttggcgttat tgaaggtgcc acagaggtcg tcaaaacaac actacagcag gttggtgtaa
    59041 agaaaagtcg tgcatttaac cacgcactat caattatgtt ggtatcgctg attaccttca
    59101 ttgtttgttg cattaacccg aacgcgattt cgatgattta cgcgatcagc ggcccgctca
    59161 ttgccatgat acttttcatc atgcctacgc tgtcaacgta tctcatcccg gcgcttaaac
    59221 cctggcgttc catcggaaat ctgattacgc tgatcgtggg tatcctgtgc gtatcggtaa
    59281 tgttctttag ctaatatccg ttgtggatgg ttcaaaattg ctacaatttt gaaccattcc
    59341 atttttcata atattcattt gttattattc cgccctatca cttctctcgt catatcctag
    59401 aattatcagt tagttaaaca cacaaagaga tctgaacatc ttaaataaga ctatttaaga
    59461 tgcataactt agattcgcaa gatatcttct ggtcattcaa aaacaatttg cgatatagat
    59521 attctcatac catgagatag ttatctggac cacgttcgac catgcaagat taaatcagtg
    59581 aaacataatc atatttaata acgagaaatg catttttaaa tatcttaatc agaattatag
    59641 caaatacaga ttaatcgcaa tttttcccag caaaattaca aaatcaaggt gtattgacat
    59701 catcaatcac caatgaaatt atgcaattat atacggatag ggaggttctt aacatggggc
    59761 tttgtagtcg ttataaaagt cttacatgca atagttgctc aatgcattgc caaataatgc
    59821 cagaagagtc accgcgtttg cagtactgcg caaactcgtg ttttgatctt acccagcaat
    59881 agtggacacg cggctaagtg agtaaactct cagtcagagg tgact
//
LOCUS       DAJTSA010000014        58573 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000014 DAJTSA010000000
VERSION     DAJTSA010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.014"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1100)
                     /locus_tag="OO369_000909"
     CDS             complement(<1..1100)
                     /locus_tag="OO369_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCR5981277.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGA"
     gene            complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="OO369_000910"
     CDS             complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="OO369_000910"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR5981278.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="OO369_000911"
     CDS             complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="OO369_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR5981279.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="OO369_000912"
     CDS             complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="OO369_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR5981280.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="OO369_000913"
     CDS             complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="OO369_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR5981281.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="OO369_000914"
     CDS             complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="OO369_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR5981282.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3404..4381
                     /gene="pspF"
                     /locus_tag="OO369_000915"
     CDS             3404..4381
                     /gene="pspF"
                     /locus_tag="OO369_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR5981283.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="OO369_000916"
     CDS             complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="OO369_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR5981284.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5804..7084)
                     /gene="puuB"
                     /locus_tag="OO369_000917"
     CDS             complement(5804..7084)
                     /gene="puuB"
                     /locus_tag="OO369_000917"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR5981285.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7086..8573)
                     /gene="puuC"
                     /locus_tag="OO369_000918"
     CDS             complement(7086..8573)
                     /gene="puuC"
                     /locus_tag="OO369_000918"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR5981286.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8723..9280)
                     /gene="puuR"
                     /locus_tag="OO369_000919"
     CDS             complement(8723..9280)
                     /gene="puuR"
                     /locus_tag="OO369_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR5981287.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9307..10071)
                     /gene="puuD"
                     /locus_tag="OO369_000920"
     CDS             complement(9307..10071)
                     /gene="puuD"
                     /locus_tag="OO369_000920"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR5981288.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10283..11701
                     /gene="puuA"
                     /locus_tag="OO369_000921"
     CDS             10283..11701
                     /gene="puuA"
                     /locus_tag="OO369_000921"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR5981289.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11850..12014
                     /locus_tag="OO369_000922"
     CDS             11850..12014
                     /locus_tag="OO369_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981290.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12004..13389
                     /gene="puuP"
                     /locus_tag="OO369_000923"
     CDS             12004..13389
                     /gene="puuP"
                     /locus_tag="OO369_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR5981291.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13523..13768
                     /locus_tag="OO369_000924"
     CDS             13523..13768
                     /locus_tag="OO369_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR5981292.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14081..15724
                     /gene="sapA"
                     /locus_tag="OO369_000925"
     CDS             14081..15724
                     /gene="sapA"
                     /locus_tag="OO369_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR5981293.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15721..16686
                     /gene="sapB"
                     /locus_tag="OO369_000926"
     CDS             15721..16686
                     /gene="sapB"
                     /locus_tag="OO369_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR5981294.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16673..17563
                     /gene="sapC"
                     /locus_tag="OO369_000927"
     CDS             16673..17563
                     /gene="sapC"
                     /locus_tag="OO369_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR5981295.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17563..18555
                     /gene="sapD"
                     /locus_tag="OO369_000928"
     CDS             17563..18555
                     /gene="sapD"
                     /locus_tag="OO369_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR5981296.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18557..19363
                     /gene="sapF"
                     /locus_tag="OO369_000929"
     CDS             18557..19363
                     /gene="sapF"
                     /locus_tag="OO369_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR5981297.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19431..19784
                     /locus_tag="OO369_000930"
     CDS             19431..19784
                     /locus_tag="OO369_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR5981298.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20152..20940
                     /gene="fabI"
                     /locus_tag="OO369_000931"
     CDS             20152..20940
                     /gene="fabI"
                     /locus_tag="OO369_000931"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR5981299.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21084..22211
                     /locus_tag="OO369_000932"
     CDS             21084..22211
                     /locus_tag="OO369_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR5981300.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22279..24213
                     /gene="rnb"
                     /locus_tag="OO369_000933"
     CDS             22279..24213
                     /gene="rnb"
                     /locus_tag="OO369_000933"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR5981301.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24291..24394)
                     /locus_tag="OO369_000934"
                     /pseudo
     CDS             complement(24291..24394)
                     /locus_tag="OO369_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24448..26422
                     /gene="pdeR"
                     /locus_tag="OO369_000935"
                     /pseudo
     CDS             24448..26422
                     /gene="pdeR"
                     /locus_tag="OO369_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26570..26743
                     /locus_tag="OO369_000936"
     CDS             26570..26743
                     /locus_tag="OO369_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981302.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26833..27582
                     /locus_tag="OO369_000937"
     CDS             26833..27582
                     /locus_tag="OO369_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5981303.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27851..28069
                     /gene="osmB"
                     /locus_tag="OO369_000938"
     CDS             27851..28069
                     /gene="osmB"
                     /locus_tag="OO369_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR5981304.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28195..28521)
                     /gene="yciH"
                     /locus_tag="OO369_000939"
     CDS             complement(28195..28521)
                     /gene="yciH"
                     /locus_tag="OO369_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR5981305.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28521..29258)
                     /gene="pyrF"
                     /locus_tag="OO369_000940"
     CDS             complement(28521..29258)
                     /gene="pyrF"
                     /locus_tag="OO369_000940"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR5981306.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29451..30620)
                     /gene="lapB"
                     /locus_tag="OO369_000941"
     CDS             complement(29451..30620)
                     /gene="lapB"
                     /locus_tag="OO369_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR5981307.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30627..30935)
                     /gene="lapA"
                     /locus_tag="OO369_000942"
     CDS             complement(30627..30935)
                     /gene="lapA"
                     /locus_tag="OO369_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR5981308.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31084..31848)
                     /gene="pgpB"
                     /locus_tag="OO369_000943"
     CDS             complement(31084..31848)
                     /gene="pgpB"
                     /locus_tag="OO369_000943"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR5981309.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32018..32608
                     /gene="ribA"
                     /locus_tag="OO369_000944"
     CDS             32018..32608
                     /gene="ribA"
                     /locus_tag="OO369_000944"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR5981310.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32672..35347)
                     /gene="acnA"
                     /locus_tag="OO369_000945"
     CDS             complement(32672..35347)
                     /gene="acnA"
                     /locus_tag="OO369_000945"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR5981311.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35511..35606)
                     /locus_tag="OO369_000946"
     CDS             complement(35511..35606)
                     /locus_tag="OO369_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981312.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35720..35887)
                     /locus_tag="OO369_000947"
     CDS             complement(35720..35887)
                     /locus_tag="OO369_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981313.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35890..36054)
                     /locus_tag="OO369_000948"
     CDS             complement(35890..36054)
                     /locus_tag="OO369_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR5981314.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(36349..37323)
                     /gene="cysB"
                     /locus_tag="OO369_000949"
     CDS             complement(36349..37323)
                     /gene="cysB"
                     /locus_tag="OO369_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR5981315.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37533..40130)
                     /gene="topA"
                     /locus_tag="OO369_000950"
     CDS             complement(37533..40130)
                     /gene="topA"
                     /locus_tag="OO369_000950"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR5981316.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40510..40761
                     /locus_tag="OO369_000951"
     CDS             40510..40761
                     /locus_tag="OO369_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR5981317.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40797..41846)
                     /gene="sohB"
                     /locus_tag="OO369_000952"
     CDS             complement(40797..41846)
                     /gene="sohB"
                     /locus_tag="OO369_000952"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR5981318.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            42066..42824
                     /locus_tag="OO369_000953"
     CDS             42066..42824
                     /locus_tag="OO369_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR5981319.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42821..43411
                     /gene="cobO"
                     /locus_tag="OO369_000954"
     CDS             42821..43411
                     /gene="cobO"
                     /locus_tag="OO369_000954"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR5981320.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(43451..44323)
                     /gene="rluB"
                     /locus_tag="OO369_000955"
     CDS             complement(43451..44323)
                     /gene="rluB"
                     /locus_tag="OO369_000955"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR5981321.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(44424..45044)
                     /locus_tag="OO369_000956"
     CDS             complement(44424..45044)
                     /locus_tag="OO369_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR5981322.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(45041..45922)
                     /gene="yciV"
                     /locus_tag="OO369_000957"
     CDS             complement(45041..45922)
                     /gene="yciV"
                     /locus_tag="OO369_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR5981323.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            46060..46104
                     /gene="trpL"
                     /locus_tag="OO369_000958"
     CDS             46060..46104
                     /gene="trpL"
                     /locus_tag="OO369_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR5981324.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    46081..46174
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            46196..47758
                     /gene="trpE"
                     /locus_tag="OO369_000959"
     CDS             46196..47758
                     /gene="trpE"
                     /locus_tag="OO369_000959"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR5981325.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            47758..49353
                     /gene="trpD"
                     /locus_tag="OO369_000960"
     CDS             47758..49353
                     /gene="trpD"
                     /locus_tag="OO369_000960"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR5981326.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            49354..50715
                     /gene="trpCF"
                     /locus_tag="OO369_000961"
     CDS             49354..50715
                     /gene="trpCF"
                     /locus_tag="OO369_000961"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR5981327.1"
                     /translation="MMQTVLAKIVADKAIWVETCKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            50727..51920
                     /gene="trpB"
                     /locus_tag="OO369_000962"
     CDS             50727..51920
                     /gene="trpB"
                     /locus_tag="OO369_000962"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR5981328.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51920..52726
                     /gene="trpA"
                     /locus_tag="OO369_000963"
     CDS             51920..52726
                     /gene="trpA"
                     /locus_tag="OO369_000963"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR5981329.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            53106..53252
                     /locus_tag="OO369_000964"
     CDS             53106..53252
                     /locus_tag="OO369_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR5981330.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGG"
     gene            53371..53871
                     /locus_tag="OO369_000965"
     CDS             53371..53871
                     /locus_tag="OO369_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5981331.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            53917..54423
                     /locus_tag="OO369_000966"
     CDS             53917..54423
                     /locus_tag="OO369_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5981332.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(54483..55121)
                     /gene="ompW"
                     /locus_tag="OO369_000967"
     CDS             complement(54483..55121)
                     /gene="ompW"
                     /locus_tag="OO369_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR5981333.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGVQQHDSVRLDPWVFMFSAGYRF"
     gene            55361..55450
                     /locus_tag="OO369_000968"
                     /pseudo
     CDS             55361..55450
                     /locus_tag="OO369_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            55478..56221
                     /locus_tag="OO369_000969"
     CDS             55478..56221
                     /locus_tag="OO369_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR5981334.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            56251..56790
                     /locus_tag="OO369_000970"
     CDS             56251..56790
                     /locus_tag="OO369_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR5981335.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            56895..57293
                     /gene="yciA"
                     /locus_tag="OO369_000971"
     CDS             56895..57293
                     /gene="yciA"
                     /locus_tag="OO369_000971"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR5981336.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(57333..58061)
                     /gene="tonB"
                     /locus_tag="OO369_000972"
     CDS             complement(57333..58061)
                     /gene="tonB"
                     /locus_tag="OO369_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR5981337.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            58276..58572
                     /locus_tag="OO369_000973"
     CDS             58276..58572
                     /locus_tag="OO369_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR5981338.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT        14064 a        15141 c        14642 g        14726 t
ORIGIN      
        1 aatgcacctt cctgctgtaa tgcctcgacc agcgctaata tttcgctttc tggaagcaag
       61 ccacgcagtg ggttcaaccc gatgccatcg tgagaggcga ggaagttaaa ccaggtcgtt
      121 ttactggagg gcaagctcag actttgcgcc cactggcaca gcgcctcgac gttctgtttt
      181 tgcaccgcat gtaacaccag cggcggcagc gagaactggt acaccatatg cgcttcgtca
      241 tcaccgttgc caaagtaagc aatgttgtct ttgtgcggaa cattggtttc ggtaataatc
      301 actgtacctg gtgctacgtc atcaataatc gaccgtaaca gcttgataat cagatgtgtt
      361 ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac ggcatccagg
      421 cggacatact cagcaccttt ctcaaggtaa cagagtaaaa catccaccat cgccagcaac
      481 acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt ccataaatgg
      541 cgcgtggaat tatcatccat ttgaaacggc gttaatagcg gtaacgcacg cggaagggtg
      601 acggcactga gatcggtttt cggatcaacc gcaataaaga aatcttcaaa acccggaagc
      661 tgttgtaaat agtttttaaa ccattcactt tttgcagaca tatggttgca gacaaaatca
      721 aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc cgcttcactg
      781 gcgacctgat gataatcaat taccgaaaat ccatcatcag atgaccatgg gtaaaaaggt
      841 aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtagaactg attaaatatg
      901 ggtagtggtt ttaaatcatt actgtgaaat tgatcggcat aggtaattaa cacgacatca
      961 ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact ctcaagacgc
     1021 gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc gtccatgtaa
     1081 tccgtaattt tctgtttcat gttgcctttc cgtggtagcg gtcccatgag gcgagagact
     1141 actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca ggaaatcatt
     1201 ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatagg gatgtaaata
     1261 aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct tttaaccttt
     1321 gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg tatatcccat
     1381 ctcgctaagg agctcttttg cctgcccgga ctggcgtccg gcattgcaat acactttcac
     1441 ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct tcaggggaat
     1501 attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa catcgatcca
     1561 gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta agcctttttt
     1621 taaacatgta agcgtccttc tatggatgaa aattgccatc cattatacgc ctgagattta
     1681 acaatttttg cgaaactccc ctcacctttt ataacgctgt gccagtttat tagcagcccg
     1741 acttaacagc ggttccagcg ccacagccag caacattttc agcggtcggc gagcaactga
     1801 ttttatcgcc cagcccgcca cacccgccgg gccatagcgc agtgcggtaa gaagtaccag
     1861 cttgcctgct aatttgaaac caagcttcac cttttgcccg gcctgttgcc agcgagtatt
     1921 cataactttc ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc ggaagtgaca
     1981 taacgttcca tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc gacttcatcc
     2041 agcaattcgc tgctggaagg tagctgctca ccaaaggcca tgttgtccgg cattggatca
     2101 agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca gcgccagacc gaaaaaaatc
     2161 gacagcacca ccaggatacg caccagtttt accggtacat caaaatagtt ggcaatcccg
     2221 gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc ataacttttt attgagatta
     2281 atgcccgcca ttagcgatcc ctccagttcg gatgttctgc atcaagaata gattccagcg
     2341 cctgaatacg ttcgcgcatc cgttttgctt catcaaccag ttgcgctaat cgctgctgct
     2401 cactttgcga caattcactg cgaccagaac gattgctgta atgcagccat aaccagatcg
     2461 gtaaaacaaa cagcacaaaa atggttaacg gaatagccag aaatagcgcg ctcataagta
     2521 ctccttagac gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt attgattgtc
     2581 ttgcttcatt ttggctttta attgtgccag ttgttcgctg attgcatcat cggctttcag
     2641 ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt ctgcttccgc
     2701 ttccatctgg tcaatacgac gttcgaaaga ttcgaaacga gccattgctt catccagttt
     2761 gccactgtcc agctgacgac gcacatcgcg cgacgagttt gccgcctgat gacgtaacat
     2821 caatgcctgc tggcgagcgc gtgtttcgct caatttgttt tccagctcgc caatctcttt
     2881 cttcatgcgt gccagcgtat cgtccaccag cgtcacttca tgttccaggg acttaatcag
     2941 atcggtcagt ttctgttttt caattaacgc tgcacgtgcc agatcctctc tctctttcag
     3001 cagcgccagt tcggcttttt cctgccattc aacttcacgc gccgacgctt gttcaatacg
     3061 gcgagtcagc tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta cttcaaccag
     3121 tgtatcttcc atctcctgga tcatcagacg caccagtttc tgtggatctt ccgctttctc
     3181 taacagagcg ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa tacccataat
     3241 gttgtcctct tgatttctgc gttcaggatt gtcctgctga actgttaata caatttgcgt
     3301 gccaattttt tatctttttg atttataaag atctgattga agaatcaaca gcaacatgcc
     3361 aggatgaatt agcgaattac actaacaagt ggcgaatttc atcatggccg aatacaaaga
     3421 taatttactt ggtgaggcga acagctttct cgaagtgctg gaacaggttt cgcatctcgc
     3481 accgctggac aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag agttgattgc
     3541 cagccgcctg cattatctct cctcccgttg gcaagggccg tttatttccc ttaactgcgc
     3601 ggcgttaaat gaaaatctgc tggattccga actgtttggt cacgaagcgg gggcgtttac
     3661 cggtgcgcaa aaacgtcatc ctgggagatt tgaacgtgcc gacggcggta cgctatttct
     3721 tgatgaactc gctacggcac cgatgatggt gcaggaaaaa ttattgcgtg tgattgagta
     3781 cggtgaactg gagcgcgttg gcggtagcca gccgttgcag gtgaatgtgc ggttggtatg
     3841 cgcgacgaat gccgatctcc cggcgatggt caatgaaggt acttttcgcg ctgacctgct
     3901 cgaccggctg gctttcgatg tagtgcaact gccgcccctg cgcgagcgcg aaagcgacat
     3961 tatgctgatg gcagaacact ttgccattca gatgtgtcgg gaaatcaagc tgcctctgtt
     4021 cccggggttt acggagcgcg ccagagaaac attgctgaat tatcgttggc cgggaaatat
     4081 tcgtgaattg aaaaacgtgg tggaacgttc agtgtatcgc cacggcacca gcgattatcc
     4141 gcttgatgac atcattattg atccctttaa acggcgtcca cctgaagaag ccatcgccgt
     4201 ttcagaaaat acctcgcttc cgacgctgcc gctggattta cgcgagtttc agatgcagca
     4261 ggaaaaagag ttgctgcaac tcagtttgca acaggggaaa tataaccaga agcgcgcggc
     4321 tgaattactg gggttaactt atcatcagtt ccgcgcgttg ttgaaaaagc accagattta
     4381 gcgaagttga gcaatacaac ttgccggatg cggcgtgaat gctttattcg gcatacataa
     4441 tcaatacgtc gtttgcaggc ctgataagcg cagcgcatca gacattattg cgttgggcta
     4501 ttaatcgctc agcgcatcct gcaaaatttt cattgccgca tcgaattgcg catccgggat
     4561 ggtcagcgga tacaggaagc gaatcacgtt gccgtatgcg ccacaggtca gcaggagcag
     4621 cccctgcgcc agcgcgcgtt gctggatttt ctgtgcaatc gccgctgacg gctcgcccgt
     4681 ttgcggatcg ttaaactctg ccgcaatcat cgaccccagg ccgcgtaccg cagcaatggc
     4741 cggaacgctt tctttggcat caatcaacgt gtttttgaga cgctggccca gttgattcgc
     4801 gcgttcgcag agtgattctt tgtcgataat attgagcacc gcgtgcgcgg cagccaccgc
     4861 cagcgggttc ccggcgtagg tgccacccaa cccgcccggc gcgggcgcgt ccataatatt
     4921 cgcgttaccg accacgcccg aaagcggcat cccgcccgcg aggcttttcg ccatcgtcat
     4981 taagtccggc ttatcggcgt aatgatccat ggcaaacagt ttaccggtac gcgcaaagcc
     5041 gctttgcact tcatcggcaa tcatcacaat accgtgctcg tcgcacaggc ggcgaatggc
     5101 ggcaaccagc tcttttggcg caacgttgaa accgcactcg ccctgcaccg gttcgaaaat
     5161 aatggccgcg acctgcttcg cttcgatgtc tgatttaaac aagcgttcga tggcgtcgag
     5221 ggagtcctgt gttgaaatgc cgtgtaaatc tgacggataa ggtacgtgat acaccgaacc
     5281 agggaacggg ccgaagccga ttttgtacgg cgcaactttt ccggtcagcg ccatggtcat
     5341 atatgtacga ccgtggaagc caccgctaaa cgcaatcacg ccaggtcgtc cggtatgggc
     5401 gcgagcaatt ttcaccgcgt tttccaccgc ttccgcgccg gtagtgaaga atgcggtttt
     5461 agcctgcccg ctcactgggg caagggtgtt aattttctcc gccagcgtga cgtagctttc
     5521 gtacggcaca atctgatacg ccgtgtgggt aaactgttgc aattgctgct ccaccgccgc
     5581 gaccagatca gggtggcgat gcccggtatt cagcaccgca atgcctgcgg cgaaatcgat
     5641 gtattcgttg ccctctacgt ccttcagcgt agcgttttct gccgactggg cgaagaagtt
     5701 acacatcacg ccaaccccgc gtggggtagc agaaagacga cgctgatgga attcattgtt
     5761 gctcatgttt cacacctttt gactcttcac tgttaatcgg aaattaaacg cccagacgat
     5821 cgcgcaggct gtaataagcc gcgcccatcg cggtaaatgg cacgcgcagc gtgcgcccgc
     5881 cggggaacgg gtaatgcggc agattggcga aggcatcgaa acgctcggcg tcaccgcgca
     5941 gcagttcagc aatcaaacgc ccggcaagat gggtacaggt gacgccgtgg ccgctgtaac
     6001 cctgcatgta atagatgttg gtatcaaggc ggccgaactg cggcatgtgc gacaaggtca
     6061 gcaggaagtt gcccgtccag cggtaatcaa ttttcacgcc cttcaactga gggaaggttt
     6121 ttagtagttt cggtaccaca agacgctcaa cgtcatccgg gttgcgtgca ccatacacta
     6181 cacccccgcc gtacagcagg cggttgtcgg cggtaagacg gtagtaatca agcagatagt
     6241 tacagtcttc cacacagtag tttttcggga tcagcgaacg ggctaaatct tccgacagcc
     6301 gttcggtggt gatcacctgc gtgccgcacg gcatgctgcg tttcgctagt tccggctcta
     6361 ctttatcgcc cagatacgca ttcccggcga caatcacata cttcgccgtc acctgaccat
     6421 tggcagtccg aaccacggct ggcgtggtgt gctggatctg cgtcacggcg gaaagctcat
     6481 acacgcgccc gccgttcagg cggatggcat cggcttcacc aatggcgagg tttagcgggt
     6541 gaatatgccc accgctgtga tccagcagcg caccagtgta gcgatcgctg gcgacctcgc
     6601 ggcgaatagc gttgacgtcc agcagctcca gctgtttatt gccgtagcgt acccagttct
     6661 ctttctgctc ttcgagtgtg gcgagttgtt tatcattcat cgccacaaac agaccgccgg
     6721 ggcggtagtc gcagtcaatc tgataacgtt tgatacgttc gcggatgatc tcgccaccct
     6781 cgaacatcat gctgccgagc atacgggcgg tgtccatgcc gtagcttttt tcgatcacgt
     6841 cgatgtcgcg gctgtaggag ttcacaagct gcccgccatt gcgcccgctt gcgccaaagc
     6901 cgatgcgtga ggcttcgaga accactacgt caaagcccgc ttccgccaga tgcagcgcgg
     6961 aggagagccc ggtatagccg ccgccaacca cgcaaacgtc gcaggtgatt gactcattca
     7021 gtgtgtcgaa tggtgcatat ttattcgcac tggcggcgta gtaactgctg gtatgttcgg
     7081 tcatttcagg cctccaggct tatccagatg gttttcagtt cagtgaattt ttcaagggca
     7141 tgcagggatt tgtcgcgacc gttgccgctc tgcttatagc cgccaaacgg cacggtcata
     7201 tcgccgtcgt tgtagttatt gacgaagacg gaaccggctt tcaggcgtcg gctcatgcgg
     7261 tgcgcgcggg agaggtcgcg cgtccatacc gccgcgccaa ggccgtactg gctgtcgttg
     7321 gcaagctgca gcgcctgatc ttctgatgtg aaacgggtga ccaccagcac cggaccgaaa
     7381 atctcttcgc ggcttaagga cgcattcggg tccacatcca cgaagatggt cgggccgatg
     7441 gccgctgcca gcccggcgtt gcgaccatcc agcagcagtt gccccttgcc ttcgccttca
     7501 cgaataaagg tgtgtaccga gtcagcgtga gcgctgtcga ttaaggtgcc catggtggtt
     7561 gcgggatcaa gtggatggcc cggctgccag ttttgcgcct gctgttttaa cagggctaag
     7621 aattcatcgg cgatgctctc ttccagtaac aaacgcgttc cggcgatgcg cacctggccc
     7681 tggttgtaga agatgcctgc ggcggtggcg cttgccgctt tttgcagatc cggacaatcg
     7741 gcgaaaacga tgttggcgct tttgccgccc gcttccagcc agacacgttt catgttgcta
     7801 tcacccgcgt ctttcagcag ttgtttaccc gtacgcgtgg agccggtaaa ggcgatggca
     7861 tcgatatcgt tatgacgcga aagcgcctgc cccgcttcat gaccaaaacc cgtcaccacg
     7921 ttcaacacac catccggcaa gcctgcttct ttcgccagcc ccgcgagacg aatcgcactg
     7981 agcggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag cgccgggccg
     8041 agtttccagc aagtcagcaa cagcgggaag ttccacggca cgatggcggc aatcacgccg
     8101 accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac ttcgccatac
     8161 actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg aatatcatca
     8221 cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc cagctcttcg
     8281 gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg ctttacgttt agccggagaa
     8341 gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc acggtcgata
     8401 tcgacgctct tgccgcgggc aattttcgcc agcggtgcct gggtgaccgg atcaacggtt
     8461 tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa gcggttttca
     8521 atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt cattatgact
     8581 cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag agatccccgg
     8641 gctgcatgcc cgtttatttc tggcgacatt ctttatggat tgccgggcgc attgcgctta
     8701 cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg ataattcggc
     8761 aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg gcataacttt
     8821 gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca ccttccagta
     8881 ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg cccggctggt
     8941 acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc agcttcattg
     9001 acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc tgcggctcat
     9061 caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc ttcagcagct
     9121 tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg atagcactgt
     9181 gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc tggcggattt
     9241 ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt ccttaaactg
     9301 tagtggtcag agtcgctgtt tttcagcgat atggtgctgc caagcggtga taaagccctc
     9361 gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc actgtacgcc
     9421 cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg gcgagcgagc
     9481 ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag agtttaccca
     9541 aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa cctgaacttc
     9601 atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat gttccagcaa
     9661 ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca ccagttcttg
     9721 taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat taattatcgc
     9781 catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt caccatatag
     9841 gtgcggctgc acattgctgg gactactagg aagataaatg ccatcgagtt tcggcaaaag
     9901 ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca agccgcctgc
     9961 gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac ccttaagcct
    10021 gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca tgctcaatct
    10081 cacaaaatgg actaaattat cgccattact gcgctttaaa tgacctttat gttcaatatt
    10141 ttttcaatct agcagtggat tttcattttt gcaaactcaa tttaacattt gacaaacatt
    10201 tggtttgcat acagattcga atgatggtca ttatatttta cgctttgata acgagcggaa
    10261 aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga actttgttca
    10321 gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct acctggagcg
    10381 ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt gcttccgtgg
    10441 aaaacgtatc cctgtttcaa gcctgaagaa gctcgagaaa gggtgttatt tcccggcctc
    10501 ggtatttgca atggatattc tgggcaacgt agtagaagaa gcaggtctgg gtcaggaaat
    10561 gggcgagccg gatcgtacct gtgttcctgt tctcggttcc ttaactcctt ctgccgccga
    10621 tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg ctccctttga
    10681 cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc gcggattgtt
    10741 cccggttgta gcggtagagc tggagttcta tttactggat cgccagcgcg acgctgaagg
    10801 ggatctgcaa ccgccctgcg cgcctggcac cgatgaccgc aatacacaaa gccaggttta
    10861 ctccgttgat aacctcaacc acttcgccga cgtgctcaat gatattgatg aactggcgca
    10921 gttgcagctg attccggcag atggcgcggt cgctgaggcc tcgccgggtc agtttgaaat
    10981 caacctttac catactgata acgtgctgga agcctgcgat gatgcgctgg cactaaaacg
    11041 cctagtgcgt ctgatggcag aaaagcataa gatgcacgcc acttttatgg cgaagccgta
    11101 tgaagagcac gcgggcagcg ggatgcatat ccatatcagt atgcaaaata atcgtggcga
    11161 gaatgtgctt tctgacacag aaggcgaaga ttcgccgctg ctgaaaaaga tgctcgccgg
    11221 gatgattgac ctgatgccgt cgtcgatggc gttgctggca ccaaacgtga actcgtatcg
    11281 ccgcttccag ccgggaatgt atgtgccgac gcaggcgtcg tggggccata acaaccgcac
    11341 cgtcgccctg cgtattccgt gcggtgaccg tcataatcac cgcgtggaat atcacgtggc
    11401 gggtgccgat gccaacccat atctggtgat ggcagcgatt tttgccggta ttttgcatgg
    11461 ccttgataac gagctgccgt tgcaggaaga agtcgaaggc aacgggctgg aacaggaagg
    11521 cttacccttc ccgattcgcc agagcgatgc cctgggtgag tttatcgaga atgatcacct
    11581 gcgccgctat ttaggcgaac ggttttgcca tgtgtatcac gcctgtaaaa atgatgagct
    11641 gttgcagttt gagcgcctca tcacagaaac agaaattgag tggatgttga aaaacgcgta
    11701 attccccttg cggggttgtt tcatcggcct gagcggagcc aggcctgaca tttgctggta
    11761 atccagacat ttcccggatg tcgcgttatg gagcgcaggc ggcaacgggc ttccgttaac
    11821 gattaagact gtgacgagga aatgagatga tgccgatgat taaatccccg catggcgagg
    11881 gaggctgcgt atgcgcccct cctgctactg actggactcc gccccctttg cttcccttgc
    11941 taaacaggtt tgatttcagg tcgacacgac cgcaaacctt attacgcagg ggaggcagca
    12001 attatggcta ttaattcacc actgaatatt gctgcgcaac ccggcaaaac ccgtctgcga
    12061 aaatcactga aattgtggca ggtggtaatg atgggtctgg cctatctcac gccgatgacc
    12121 gtatttgata cttttggtat tgtctccggc attagcgacg gtcacgttcc ggcgtcctat
    12181 ttgctggcgc tggcgggcgt gctgtttacc gctatcagct acggcaaact ggttcgccag
    12241 tttccggagg ccggttcggc ctatacctac gcgcaaaagt cgattaaccc gcacgtcgga
    12301 tttatggtcg gctggtcatc actgctggat tatctctttt tgccaatgat caacgttttg
    12361 ttggcgaaaa tctatctctc cgccctcttc ccggaagtgc cgccgtgggt gtgggtggta
    12421 accttcgtcg ccattttaac cgccgcgaat ctgaagagcg tcaacctggt cgctaacttc
    12481 aataccctgt ttgtactggt gcaaatctcc atcatggtgg tgtttatctt cctggtggtt
    12541 cagggactgc ataaaggaga aggcgttggc accgtctggt cacttcagcc gtttatcagc
    12601 gagaacgcgc acctgatccc gattattacc ggtgcgacga ttgtctgttt ctcgttcctc
    12661 ggtttcgatg cggtgaccac gctttcggaa gagacgccgg acgccgcacg cgtgatcccg
    12721 aaagccatct tcctgacggc ggtatacggc ggcgttatct ttatcgcggc gtcgttcttt
    12781 atgcagctgt tctttcccga tatcagccgc tttaaagacc cggacgccgc actgcctgaa
    12841 attgcgctgt acgtcggcgg caagctcttt cagtcgattt tcctctgcac cacgtttgtg
    12901 aacacattag cgtctggcct ggcttcacat gccagcgtgt cgcgtctgct gtatgtgatg
    12961 gggcgtgaca atgtgtttcc ggagcgcgtg tttggctatg tgcacccaaa atggcggact
    13021 ccggcactca acgtcattat ggtcgggatt gtcgcgctgt cggcgctgtt ctttgattta
    13081 gtcaccgcga cagcactgat taacttcggt gctctggtgg cgtttacctt cgtgaatctg
    13141 tcggtgttta atcatttctg gcggcgtaaa ggaatgaata aaagctggaa ggatcacttc
    13201 cactatttgc tgatgccgct ggttggcgcg ctgacggtgg gtgtgctgtg ggttaaccta
    13261 gagtcaacgt cactgacact cggtctggta tgggcttcgt taggcggcgc atatttgtgg
    13321 tatctgatcc gccgctatcg caaagtgccg ctgtacgaag gtgacagaac gccggtgagc
    13381 gaaacgtaat agccggatgc gcggcgcatt atcctgcctg agggtaacgc gccgcgccat
    13441 taactataaa aagatgtatg acccggatca cgagtgctat catccgccct tatgcttagc
    13501 gcattaaccc caggggcaaa taatgaacca cgatatcccg ctaaaatatt ttgatattgc
    13561 cgatgagtac gcgaccgaat gcgcagagcc tgtcgcagag gctgaacgca caccgctggc
    13621 ccactacttc cagttactgc tcacccgttt gatgaacaat gaagagatca gcgaagaagc
    13681 ccaacatgaa atggctgctg aagcggggat taatcccgta cgcattgatg agatcgcaga
    13741 gtttttgaat caatggggta atgagtaatt atttaattgc agacgatcgg tcagtatcgt
    13801 tgtccggata aagcgttcac gccgcatccg gctaacaatg acacgcactg cagcgtgctt
    13861 taatgggttt ctcacgatcg taccgctctg ctcctcgcta agttgttctg tgagttatag
    13921 ttatagagaa atcacgggta cgaatgttga acagatggcg acgccacctg ttcaacgacc
    13981 aaccacacgg ccctcattac gacccctgaa cacaaacggt aatggcaaat gtcattaaat
    14041 attagtaaga tagcctcttt acataaaatc ccttaatatt atgcgccagg tattatcgtc
    14101 tcttttggtg attgctggac ttgtgagtgg tcaggcaatc gccgcgcctg aatccccccc
    14161 gcatgctgat atccgcgaca gcggttttgt ctattgcgtc agcgggcaag tcaacacctt
    14221 taacccatcc aaagcgagca gtgggttaat tgtcgatacc cttgccgccc agttttatga
    14281 tcgactgctg gatgtcgatc cctataccta tcgcctgatg ccggaacttg ccgaaagctg
    14341 ggaagtactc gacaacggcg cgacctatcg cttccacctg cgtcgcgatg ttccgtttca
    14401 aaaaaccgac tggtttactc ccactcgtaa aatgaatgcc gacgatgtgg tgtttacctt
    14461 ccagcgaatt tttgaccgca acaacccgtg gcataacgtc aacggcagca acttccccta
    14521 cttcgacagc ctgcaatttg ccgataacgt gaaaagcgtc cgcaaactgg ataatcatac
    14581 cgttgagttc cgactcgccc agccggatgc ttcttttttg tggcacctcg caacccatta
    14641 tgcttcggtc atgtcggcag aatatgcccg gaagttagag aaagaagatc gccaggagca
    14701 actcgaccgt caaccggtcg gcaccggtcc gtatcagttg tcggaatacc gcgccgggca
    14761 atttattcgc ctacaacgtc atgatgactt ctggcgcggt aaaccgttaa tgccgcaggt
    14821 agtggtggat ttaggctccg gcggcaccgg acgtctgtcg aaactcctga ccggggaatg
    14881 cgacgttctg gcctggcctg ctgccagcca gctatccatt ttgcgtgacg acccgcgctt
    14941 gcgtttaacg ctgcgtcctg ggatgaacgt cgcctatctg gcatttaaca ccgccaaacc
    15001 gccgctaaat aatcccgctg tccgccatgc actggcactg gcgattaata accagcgcct
    15061 gatgcaatcc atctattatg gtacggctga aacggcggcc tctattttac cgcgcgcctc
    15121 gtgggcctat gacaacgagg ctaaaattac tgaatacaat ccggcgaaat cgcgcgaaca
    15181 gttgaaggcg ttggggctgg aaaacttaac gctgaaactg tgggtgccca cacgttcgca
    15241 ggcgtggaac cccagtccac tgaaaactgc cgaactgatt caggcggata tggcgcaggt
    15301 tggcgtaaaa gtggtgattg tgccggtaga aggtcgcttt caggaggcgc ggttgatgga
    15361 tatgagccat gatctgacgt tatccggttg ggcgacggac agtaacgacc cggacagttt
    15421 cttccgtccg ttactgagct gcgcggcaat tcattcacag accaacctcg cccactggtg
    15481 caatccgaaa ttcgacagcg tgttgcgtaa ggcgctctcc tcgcagcagc tggcagcgcg
    15541 tattgaagcc tatgacgaag cgcagagtat tctggcgcag gaattaccta ttctgccgct
    15601 ggcgtcgtca ttgcgtttgc aggcctatcg gtacgatatc aaaggtctgg tacttagccc
    15661 gtttggtaac gcctcctttg ctggggtgta tcgcgagaaa caggatgagg tgaaaaaacc
    15721 atgattatct tcaccttacg ccgcattttg ttattgattg tcaccttgtt cctgctgacc
    15781 ttcgttggct tcagcttgag ctatttcact ccacacgcgc cgttacaagg cgcgtcactg
    15841 tggaatgcct gggtgttctg gtttaatggc ctgatccact gggattttgg tgtttccagc
    15901 attaacggtc aaccaattgc cgaacagtta aaagagctct tcccggcgac gatagaactg
    15961 tgcatcctcg ccttcggttt tgcgctgatt gttggcatcc ccgtggggat gattgccggg
    16021 attacgcgcc ataagtggca ggataacttg atcaacgcca tcgccttact gggcttttca
    16081 atcccggtgt tctggctggc gcttctgttg acactgtttt gttcactcac gctaggttgg
    16141 ttgcccgttt cagggcgttt cgatctgctc tacgaagtga aaccgattac cggttttgcg
    16201 ttgattgatg cctggctttc ggactcacca tggcgggatg aaatgatcat gagcgcaatc
    16261 cgccatatga tattgcccgt gatcaccctg tcggttgcgc caacaactga agtgatccgc
    16321 ctgatgcgta tcagcactat tgaagtgtat gaccagaact atgtaaaagc ggcggcgacc
    16381 cgcggtttgt cgcgctttac tattttgcgt cgccacgttc tgcataacgc gttaccgccg
    16441 gttattcctc gcctgggctt acagttttcc accatgctga cgctggcgat gatcaccgaa
    16501 atggttttta gctggccggg tttagggcgc tggttaatta acgccatccg ccagcaggat
    16561 tacgcagcca tttccgccgg agtgatggtg tgtggctcac tggtcattat tgttaacgtg
    16621 atttctgata ttttgggtgc catggctaac cctctgaaac ataaggaatg gtatgcctta
    16681 cgatagcgta tacagcgaaa agcgcccgcc gggcacgctg cgtaccgcct ggcgcaaatt
    16741 ttatagtgac gcctctgcga tggtcggcct gtacggctgc gcgggactgg ctgtactgtg
    16801 tatttttggc ggctggtttg cgccttacgg catcgaccag caatttctcg gttatcaatt
    16861 attgccgcca tcatggtcgc gctatggcga agtttctttc ttcctgggga ctgacgacct
    16921 cggacgtgat gttttaagcc gtttactgag cggagctgcg ccaaccgtgg gtggcgcatt
    16981 tgtggtgacg cttgccgcga cgatctgtgg cctggtactg ggcaccttcg ccggggcgac
    17041 acacggcctc cgctcagcgg tgcttaacca tattctggat accttactgg cgatcccttc
    17101 gctgctgttg gcaattatcg ttgtagcgtt tgccggaccg agtttgtccc acgctatgtt
    17161 tgctgtctgg ctggcattgc tgccgcgtat ggtacgttcg atttacagca tggtgcatga
    17221 cgaactggaa aaagagtacg ttatcgccgc ccgtctggat ggcgcatcaa cgctgaatat
    17281 tctctggttt gctgtgatgc caaacatcac cgccgggctg gtcaccgaga tcacccgcgc
    17341 actgtcgatg gcaattctcg atatcgccgc gctgggcttt ctcgatctcg gcgcacaact
    17401 cccctcgcct gaatggggag cgatgctcgg tgatgcgctg gaactgattt atgtcgcccc
    17461 gtggactgtc atgctgccag gtgcggcaat tatgattagc gtgttgttgg ttaacctcct
    17521 tggcgatggc gttcgtcgtg cgattattgc gggggtggaa taatgccatt actcgatatt
    17581 cgtaacctga ccattgaatt taaaaccggt gatgagtggg ttaaagccgt cgaccgggta
    17641 agtatgacct taaccgaagg tgaaatccgc ggtcttgttg gtgaatccgg ttccggcaag
    17701 agtttgattg cgaaagcgat ttgtggggtg aataaagata actggcgtgt tactgctgac
    17761 cgtatgcgtt ttgatgatat cgatttgctg cgtctctccg cacgcgaacg gcgcaaactg
    17821 gttggccata acgtgtcgat gattttccag gaaccgcagt cgtgtcttga cccttcagaa
    17881 cgtgtgggtc gccagttgat gcaaaacatc ccagcctgga cctataaagg ccgttggtgg
    17941 cagcgttttg gctggcgcaa acgccgtgcc attgaactgc tgcaccgcgt ggggattaaa
    18001 gatcacaaag atgcgatgcg cagttttccc tatgagttga ccgaaggtga atgtcagaaa
    18061 gtgatgatag ccattgcact ggcgaatcaa ccgcgtctgc tgattgctga cgaaccgaca
    18121 aactcaatgg agccaacaac ccaggcgcaa atctttcgcc tgctgacgcg tctcaaccaa
    18181 aacagcaata ccactatttt gcttatcagc catgacttac aaatgcttag ccaatgggcg
    18241 gataaaatta acgtgcttta ctgcggtcaa acagtggaaa ccgcgccgag taaggagttg
    18301 gtgacgatgc cacatcatcc ttatacccag gcgctgatcc gcgcgatacc agacttcggc
    18361 agcgcgatgc cgcataaaag tcgcctcaat actctacccg gcgctatccc attgctggaa
    18421 cagttaccga ttggttgtcg tctggggcca cgttgcccgt atgcacaacg agaatgcatt
    18481 gtgacgccac gtttgacggg ggcgaaaaat catctctatg cctgtcattt cccgctgaac
    18541 atggagaaag agtgagatga tcgaaacgct gcttgaagtg cgtaatttaa gtaaaacgtt
    18601 ccgctaccgg accggttggt ttcgtcgtca gaccgtagaa gcggtaaaac ccttgagctt
    18661 tacgctacgt gaaggccaga cactggcgat tattggcgag aatggttcgg gtaaatccac
    18721 actggcaaaa atgctggcgg gaatgataga acctaccagc ggcgagttat tgattgacga
    18781 tcatccgctg cattttggcg attattcctt ccgtagtcag cgtattcgca tgatttttca
    18841 ggatccttcg acctcgttga atccccgtca acgtatctca caaattctgg attttccact
    18901 gcgcctgaac accgacctgg aaccggaaca gcgtcgcaaa cagattattg aaacgatgcg
    18961 tatggttggg ctgctaccgg atcacgtcag ttactatccg catatgttgg cacccggaca
    19021 aaaacagcgt ctgggtctgg cgcgcgcgct gatattgcgc ccaaaagtca ttattgccga
    19081 tgaagccctc gcttcactgg atatgtcaat gcgttcgcag ttgattaatc tgatgctgga
    19141 gttacaggaa aaacagggca tttcgtatat ttatgttacc cagcatatcg gaatgatgaa
    19201 gcacattagc gaccaggtgc tggtgatgca tcagggcgag gttgtcgagc gaggcagcac
    19261 cgcggatgtg ctggcatcgc cgctgcatga actcaccaaa cggctgattg ccgggcactt
    19321 tggcgaagca ttgacagcgg atgcgtggcg taaagatcgc taattttctg aatcctcctt
    19381 ccagattatt cacctcattc tgacaagaga ttaaatcttg tcagaatgag gtgatggata
    19441 aaataaaatc aaatgcgcgt gatttaagac gcaatctcac tttgcaggaa cgaaagctct
    19501 ggcgatatct tcgcagccga cgttttggtg atttcaaatt tcgccgtcaa catccggtgg
    19561 ggagctacat tctcgatttt gcttgctgct cggcgcgcgt agtcgttgag ctggatggtg
    19621 ggcagcatga tttggcagtt gcctatgata ccaggcgcac tagctggctt gagtcgcagg
    19681 gctggaccgt gctgcgtttc tggaataacg agattgattg taatgaggag gcggtgctgg
    19741 agattattct gcaggaactg aaccgccggt caccctcacc ctgaaagggc gagggggccg
    19801 accgagccga atagctgttg tggtgaaaac atggagacag tgctggagaa tattcggcaa
    19861 ggtctgaacc atcccagcca tcgccataaa agggttaggg gctgtatgag cctgtttgtt
    19921 gctggggtaa caatatttgc acaatatggt cccctcgccc ctctggggag agggttaggg
    19981 taaggggaaa agcgcccccc ctgccgcagc ctgctccggt cggacctggt aactatagct
    20041 actcacagcc aggttgatta taataaccgt ttatctgttc gtactgttta ctaaaacgac
    20101 gaatcgcctg attttcaggc acaacaagca tcaacaataa ggattaaagc tatgggtttt
    20161 ctttccggta agcgcattct ggtaaccggt gttgccagca aactgtccat cgcctacggt
    20221 atcgctcagg cgatgcaccg cgaaggagct gaactggcat tcacctacca gaacgacaaa
    20281 ctgaaaggcc gcgtagaaga atttgccgct caattgggtt ctgacatcgt tctgcagtgc
    20341 gatgttgcag aagatgccag catcgacacc atgttcgctg aactggggaa agtttggccg
    20401 aaatttgacg gtttcgtaca ctctattggt tttgcacctg gcgatcagct ggatggtgac
    20461 tatgttaacg ccgttacccg tgaaggcttc aaaattgccc acgacatcag ctcctacagc
    20521 ttcgttgcaa tggcaaaagc ttgccgctcc atgctgaatc cgggttctgc cctgctgacc
    20581 ctttcctacc ttggcgctga gcgcgctatc ccgaactaca acgttatggg tctggcaaaa
    20641 gcgtctctgg aagcgaacgt gcgctatatg gcgaacgcga tgggtccgga aggtgtgcgt
    20701 gttaacgcca tctctgctgg tccgatccgt actctggcgg cctccggtat caaagacttc
    20761 cgcaaaatgc tggctcattg cgaagccgtt accccgattc gccgtacagt tactattgaa
    20821 gatgtgggta actctgcggc attcctgtgc tccgatctct ctgccggtat ctccggtgaa
    20881 gtagtccacg ttgacggcgg tttcagcatc gctgcaatga acgaactcga actgaaataa
    20941 tcgttctgtt ggtaaagatg ggcggcgttc tgccgcccgt tatctctgtt atacctttct
    21001 gatatttgtt atcgccgatc cgtctttctc cccttcccgc cttgcgtcag gataacgatt
    21061 tcctttacga ccaaggagcg cccatggaac aacgccacat caccggcaaa agccactggt
    21121 atcatgaaac gcaatccagt actacggagt atgacgttct gcctctggtc ccggaagccg
    21181 caaaggtcag cgatcccttt ctgctcgacg tgatccttga tgaagagacg ctggccccct
    21241 tcctttcatg gctggtccct gcgcgcgttc ttgcagtgga gttgttccct gaccagctta
    21301 ccgtgacccg ttcacaaacc ttcaccgctt atgaacgctt gtcgacggcc ctgacggttg
    21361 ctcaggtttg cggcgtccag cggttatgta actactattc ggcgcgactt acgccgctcc
    21421 ccggacctga ttcctccagg gaaagtaatc atcggttggc acaaatcacg caatatgccc
    21481 gccaactggc tagctcgcct tctattatcg acaaccgatc gcgccagcat ctgaatgacg
    21541 tcggtcttac tgtctgggac tgtgtgatca ttaaccaaat cattggtttt attggctttc
    21601 aggcgcggac aattgcgaca tttcaggctt atctcgggca tccggtacgc tggttacccg
    21661 ggctggcgat acaaaactac gccgacgcgt cactgtttgc tgatgaatca atacgctggc
    21721 gaagcagcta tgaggtggaa aaactacctg aagagcacac aaaaagttca actgcagaac
    21781 tttgccaact ggccgaaaca ctctctctcc accctatttc actttccctt ctcgaaaagt
    21841 tgttaaacag cacacgggtc aatgcacagc cggataatca gcttgcggcg ttgttatgcg
    21901 cgcgtataaa tggcagtcct gcttgttttt ccacctgtat ggattcatca aatgaatata
    21961 aaaaaatcag cacccttctg cgcaagggcg aaaatgaaat taaccgatgg gctgaccgtc
    22021 attctgttga gcgcgctacc gttcaggcga tacaatggct gacccgagca cccgatcgct
    22081 ttagcgccgc ccagttcagc cctttactcg aacacgaaaa atcatcaacg cagattatta
    22141 atctgctggt atggagcggg ctgtgtggct ggataaatcg cttaaaaatc gcgttgggtg
    22201 agacatatta accttgccgc gtcagacaga ttcgcgtaaa actgtcagcc gctctaatgg
    22261 ccaccaaaat agacaattat gtttcaggac aacccgctgc tagcgcagct taaacagcaa
    22321 ctgcattccc agacgccacg cgctgaaggg gtggtaaaag ccacagaaaa aggctttggc
    22381 ttcctggaag tcgacgcaca aaaaagttat ttcattccgc cgccgcagat gaaaaaagtc
    22441 atgcatggcg accgaattat cgcggtgatc cacagtgaaa aagaacgtga atccgcagag
    22501 ccagaagaac tggttgaacc gttcctgact cgtttcgtgg gtaaagttca gggcaaaaat
    22561 gaccgtctga ccatcgttcc tgatcatcca cttttaaaag acgccattcc ttgccgcgca
    22621 gcccgtggcc tgaaccacga gtttaaagaa ggcgactggg cggttgccga aatgcgccgt
    22681 catccgctga aaggcgatcg ttctttctat gctgaactga cacaatacat cacttttggt
    22741 gacgaccact ttgtaccgtg gtgggttacc cttgcgcgcc ataatctgga aaaagaagca
    22801 ccagacggcg tcgctaccga aatgctcgat gaaggtctgg ttcgtgaaga tctgacctcg
    22861 ctggattttg tcaccatcga cagtgccagc actgaagata tggatgacgc ccttttcgct
    22921 aaggcgctgc cggatgacaa acttcagctg attgtggcga ttgccgatcc aaccgcgtgg
    22981 attgctgaag gcagcaagct ggacaaagcc gcgaaaattc gcgcattcac caactatctg
    23041 cctggcttca acatccctat gctgcctcgc gagctttctg acgatctctg ctcactgcgc
    23101 gccaatgaag tccgcccggt actggcatgc cgcatgacgc tctccgctga tggcaccatt
    23161 gaagataata tcgaattctt tgccgccacc atcgaatcca aagcgaagct ggtgtatgac
    23221 caggtttctg actggctgga aaataccggt gactggaagc ctgaaagtga agcaattgcc
    23281 gaacaagtcc gtttgctagc gcaaatttgc caacgccgcg gcgagtggcg tcataaccac
    23341 gcactggtgt ttaaagatcg cccggattac cgctttattc tcggtgaaaa aggtgaagtg
    23401 ctggatatcg tcgccgagcc tcgtcgcatt gccaaccgta tcgtcgaaga agcgatgatt
    23461 gccgctaaca tttgtgcggc ccgcgtactg cgcgataagc tcggttttgg catctataac
    23521 gtgcatatgg gctttgatcc ggcgaatgcc gacgcgctgg cagcgttgct gaaaacgcac
    23581 ggtctgcatg tcgatgccga agaagtgctc acgctggacg gtttctgcaa actgcgtcgt
    23641 gaactggatg cgcaaccaac tggtttcctc gacagccgca ttcgtcgctt ccagtcattt
    23701 gctgaaatta gctctgaacc cggtcctcac tttggcctcg gtctggaagc atacgccacc
    23761 tggacttcgc cgatccgtaa atatggcgac atgatcaacc accgtctgct gaaagcggtt
    23821 atcaaaggcg aaactgcgac gcgtccgcag gatgaaatca ctgtccaaat ggccgaacgt
    23881 cgccgtctca accggatggc agaacgtgat gttggcgact ggttatacgc acgcttcctg
    23941 aaagataaag ccgggaccgg cacccgtttc gcggcggaaa ttgtcgatat cagccgtggc
    24001 ggcatgcgtg tgcgtttggt tgataacggc gctatcgcct ttattccggc acctttctta
    24061 cacgctgtgc gcgatgaact ggtttgcagc caggaaaacg gcactgtaca aattaaaggt
    24121 gaaacggttt acaaagtaac tgacgttatt gacgtcacca ttgccgaagt ccgcatggaa
    24181 acccgcagca ttattgcgcg cccggtcgcg taatctcctt tcacggccca ttcctcatgg
    24241 atgggccgtt tatttccccg ctctaccttc gttatatcca ggcagatttt ttaactgact
    24301 ttcgtttgaa aactggcgtt tttccaaaac tggattacaa ataaatatat aaaaaaacaa
    24361 tgcgtttagc cgccgttaat ttcatgtaag tcatgttgtt ccttttattt aaccgcggac
    24421 tccgctgtta accggaggat atgcatcatg aaaaccgtta gggagtccac aacgttgtac
    24481 aactttctcg gatcgcacaa tccatactgg cggttgacgg aaagcagcga tgctttgcgc
    24541 ttttctacca ccaaaaccac agaacctgat cgtacattgc agttatctgc cgaacaggct
    24601 gctcacatca gggaaatgac ggtcatcacc tccagcctga tgatgagtct gaccgtcgat
    24661 gaaagcgatc tttctgtgca tctggtagga cgaaaaatca ataaacggga atgggctggc
    24721 aacgcgtctg catggcatga cacaccggcg gttgctcgtg atttatcaca cgggctttcc
    24781 tttgctgagc aggtagtttc tgaagcacat tccgcaatag tgattctcga cagccggggg
    24841 aatatccaac gcttcaatcg gttatgtgaa gattacacag ggttgaaaga acacgacgtc
    24901 attgggtaaa gcgtgtttaa actgtttatg agccgtcgtg aagctgcggc atccaggcgc
    24961 aataaccgtg tattttttcg aagcggcaat gcatatgaag tcgaactgtg gataccaaca
    25021 cgtaaaggcc agcggctgtt tctgtttcgc aataaatttg tccacagcgg cagtggcaaa
    25081 aacgagattt ttttaatctg ttccggtacc gacattaccg aagagcgccg cactcaggag
    25141 cgactgcgta ttctggcaaa taccgacagt atcaccggac tgccgaatcg taacgcaatg
    25201 caggagttaa tcgatcacgc tattaatcag gcagataaca ataaagttgg ggttgtgtat
    25261 cttgatttgg ataattttaa aaaggtcaac gacgcctatg ggcatttgtt tggtgaccag
    25321 ttattacgcg acgtgtcatt ggctatttta agctgtctcg aacatgacca ggtgttggcg
    25381 cgtccaggtg gcgatgagtt tctggtactg gcatccaata cctcacaaag cgcgctggaa
    25441 gcaatggcat cacgaatttt gacccgctta cgcctcccct ttcgtattgg tttaattgaa
    25501 gtttatacca gctgttcagt aggtatttcg ctctctcccg aacatggttc ggacagcgcg
    25561 actattattc gtcacgccga cacagcaatg tacacagcga aggaaggcgg acgaggacaa
    25621 ttttgcgttt ttactccaga aatgaatcag cgggtatttg aatatctctg gctggatacc
    25681 aacttgcgta aagcactgga aaacgatcag ttggttatcg aaaatcacct agcgtgtcga
    25741 agtgcgcagt ctggaagcac tagtacgttg gcagtcacct gaacgtgggt tgattccacc
    25801 gttggacttc atttcctacg ccgaagagtc agggctaatt gtgcctttag gccgttgggt
    25861 gattctcgat gtcgtatgcc aggtggcaaa gtggcgggat aaaggcataa acctgcgagt
    25921 ggcggtaaat atttctgcac gtcagctcgc cgatcaaacc attttcaccg ccctgaaaca
    25981 ggttctccag gaactcaatt ttgaatactg ccctatagat gttgaactga cagagagttg
    26041 tctgattgag aatgatgaac tggcactgtc tgttattcaa caatttagcc aactaggtgc
    26101 gcaagtgcat ctggatgatt ttggtaccgg ctactcttca ctttcgcaac tggcgcgctt
    26161 tccgatcgat gccatcaaac ttgaccaggt ttttgttcga gatattcaca aacaacctgt
    26221 ctcgcagtca ctggtccggg cgatcgtcgc tgtggcccag gcattgaatc ttcaggtgat
    26281 cgccgaaggt gtagagagtg caaaggaaga tgctttttta accaagaacg ggatcaatga
    26341 gcggcaagga tttttgtttg ccaaaccgat gcccgccgtc gccttcgaac gctggtataa
    26401 acgctatctg aagcgcgcat aagtagatga atatatatac ttagcgctat atcccgtcat
    26461 tatatatcgt attcctggat gataattagc cgttgctgtt aaacacttct cttttagcgg
    26521 caatttttgc attattcatt ttcccccttt tgctataagg atgtccttga tgtctgaatt
    26581 tgatgcccag cgcgttgccg aacgtattga tattgtgtta gatatccttg tggcaggcga
    26641 ttaccactct gcgatccata atctcgagat acttaaagca gaactcttgc gccaggttgc
    26701 tgaatctacg ccagacatcc cgaagacacc gtgggagatt taacaatcgt gccttcaggt
    26761 tttcctattt gaggatgccc ggcgcgtcag tattttttta tttagtatta taacgttata
    26821 agaattacag cgatgaattc ccgacaacaa actattctac agatggtcat tgaccagggt
    26881 caggttagcg taaccgatct ggcaaaagcc actggagttt ctgaagttac cattcgccag
    26941 gatctcaaca ccctcgaaaa actgagttac ctccgccgtg cacatggctt tgcagtttcg
    27001 cttgatagtg atgacgtcga aacccgtatg atgagcaact atacgctgaa gcgtgaactc
    27061 gccgagtttg ccgcgtcact ggttcaaccg ggcgaaacca tctttatcga aaatggcagc
    27121 agcaatgccc tgctcgcccg gactctgggg gagcagaaga aaaatgtcac tatcatcacg
    27181 gtcagcagct acatcgcgca tttgctgaaa gacgcgcctt gtgaagtcat tttgctcggt
    27241 ggcgtgtacc agaaaaaaag cgaaagtatg gttggccctt tgacacgcca gtgcatccag
    27301 caggtacatt tcagcaaagc atttattggt attgatggct ggcaacctga aactggattc
    27361 accggtcgcg atatgatgcg caccgatgtg gtcaatgccg tgctggaaaa agagtgcgaa
    27421 gcgatagtcc tgactgacag ctcgaaattt ggtgctgtac attcatactc catcggtccc
    27481 gttgagcgat tcaatcgcgt gattaccgat tcgaaaatac gcgccagcga tctgatgcat
    27541 cttgagcaca gtaaactcac cgttcacgtc gttgacattt aaccatttcc cttctctacg
    27601 gatgatttgc agtttggcaa atcatccgct ctaagatgat tcctggttga taattaagac
    27661 tatttacctg ttattaacac tctcaagata taaaattatt atcagcgata taacaggaag
    27721 taattatcac ctgcgtgata taaccctgcg cgcgagcaga tttcacggaa taatttcacc
    27781 aggcttattc ttagctatta tagttataga gagcttactt ccgtgaatca taaattcagg
    27841 agagagtatt atgtttgtaa cgagcaaaaa aatgaccgcg gctgttctgg caattacttt
    27901 ggcaatgtct ctgagtgcct gttctaactg gtctaaacgg gaccgcaaca ccgcaatcgg
    27961 cgcgggtgca ggggcattag gcggtgcagt actgaccgat ggcagtacgt tgggtacatt
    28021 aggtggtgca gctgtcggtg gtgttattgg tcaccaggtc ggtaaataat cgtaaataaa
    28081 tgtcgattat cgactcgcaa ttataatacg cttgattctg ttcagtacga aataactgaa
    28141 taatacacac agtaaaatat tcgaaagcca cggatatatc cgtggctttt tatgttaacc
    28201 gcctgcgagt tttaccttca tccctttcgc ttcaagtagt gattttaata aatcacgctt
    28261 atcgccctgg atttcaataa ctccatcttt aactgctccg ccgcagccgc attttttctt
    28321 cagttccgct gcaagttttg tcagttcggc atcatcgaga tcgataccgg taatcaggca
    28381 aacgccctta cctttacgtc cactggtctg acgctgaata cgcaccgctc catcgccttt
    28441 agggcgtact ggggccgctt tgggctcatc aatacgcccg gtttccgttg agtagaccag
    28501 acggctgttg gaatcactca tcatgcactc cgctgtaaag aggcgttgat cgctttcagt
    28561 gtctgcgctg gatctaccga ttgcgttacc gggcgaccaa tcaccatata atcaacacca
    28621 gccgccaacg cctgttctgg cgtcataatg cggcgctggt caccagcttc actcccctgc
    28681 ggacgaatgc ccggtgtaac cagtttgaac tcctgaccga atacctgttt aaagcgcaca
    28741 gcttcctgag cagaacacac cacaccatca aggtcacatt tttgcgtcag tgctgccaga
    28801 cgttctgcat aatctgcagg tgacagtgtc acgccaagat cggccaggtc gctggcttcc
    28861 atgctggtca acactgtcac agcaatcaaa agcggtgcat ctttgccaaa cggaaccagt
    28921 gcctcacgcg ctgcggtcat catacgcgcc ccaccggagg catgaacatt caccatccac
    28981 acgcctaagt cagctgcagc agcgacagcg tgcgctgcag tgttggggat atcgtggaat
    29041 ttcaggtcaa gaaagatatc aaaaccacgc tgttgaagtt cgcgcacaaa ctgtggccca
    29101 aacaatgtaa acatctcttt accgaccttc agacgacaat cgcgtgggtc gatcttgtcg
    29161 acaaaggaca gcgcgtcatc acaattatga taatcaaggg caacaaccac aggagaattc
    29221 gtaacggcgc gggaagaaga tgaagcagtt aacgtcatga ccagaccttc ttgatgatgg
    29281 gcaccgggag tggcgcgaaa caggtaaacg gcgagcattc tacccgtgaa aataaaaaat
    29341 taacagttgc gatttttccc ctagcaggct ttttctgctc cgtcgttgcc ggagcacaat
    29401 gggacatcaa taactagtat gttgtaacta aagtcggatt tttttaaaaa ttacaggcca
    29461 tcaagaccgc gaatcggttt aatagttgac caggcacgac aagacggaca atgccagtag
    29521 agggtgtatg cggtaaaacc acatttctgg cagctataac gaggcttact acgcaccttc
    29581 tcgccaacca tgtcacgcag caccatcaga ctctctttgg cgcgcccttc ttccgcttca
    29641 tttaagtggt aatccattag cttatggaat acacgcatgg tcggatgacg ctgaagctgg
    29701 cgtgtaatat agacctgtgc agcctcacta ccgtcgcgcg cttcgatgat atcagcaagc
    29761 atcaattcag catcggcacc ggtgttctct tccaccgcgc gttgcaggaa ttctgcccat
    29821 tcggcagttt tacccaactg ctgatagcag gtttgcaaca tttccagcgt ttcgctgacc
    29881 agttctctgt cctgggatat gacgcgttgc aaactttcga cggctttggc gtattctcct
    29941 ttcgccataa acacgcgtcc catcatgatg gatacgcggg cgctgttttt atctgccgcc
    30001 gccccttttt ttagcaaggt catggcacga tcgagatcgt cgctggccat atgctgcagg
    30061 gctaactcac agtagaaatg ggcaatttcg acgcgctgtt tatctttacc cagcttcacc
    30121 aggcgttcgg caacatcaat tgctttctgc cactcactgg tagcctggta gatttgtagc
    30181 aactgttgca gcgcgccaat gcggaagtca gtttcatcgg tcagctgatt gaacatgtct
    30241 tccgcgcggt cgtataaccc ggcggccatg taatcacgcc ccagttgttg aatcgccaac
    30301 agacgctgtt cataggtcag cgaggcgctt tccattaggg tctgatggat gcgaatagcg
    30361 cgatcaactt cgccacgcga acggaacagg tttccgagcg taaggtgggc ttcaacggtg
    30421 cctgtatcct ctttaagcat atcgagaaac aggtctaccg ctttatcctg ttgattacta
    30481 agcaggaagt taacccccgc tacgtaatca cgcgacaagc ggttggcttc atcttgcttg
    30541 ttttgttgcg cacttctgcg gcccatatac cagccatagg cagcggctac aggcaaaagc
    30601 agaaacaaca actccagcat agaaagttat tccttcgccg ctgacgagtg cggcactaca
    30661 gccacgtcag tcgcgggtga aagctggttt tccagtcgct ttattttacg ttcagcgcgc
    30721 gccagggaaa cacgaactcg cagccagaac aggccacaaa tcaaccaacc gatagcaaac
    30781 cccgcagcaa acaataccgc cagcaatgtg gaaatacggt actccccttg cgctaacaga
    30841 taattaaacg tcacctgttg atcgttctgc gcacccaacg tgaccgaaat cacgaagatc
    30901 gctaacacca gtaagaaaat gagtaaatat ttcacattac ttcccgttat gtggttagag
    30961 cgaataaatt atggtcaact taccgcaata gccctttaaa gtaccatttt cgcgccgtct
    31021 gcgaaatggc atccacgcgc attgttgatg atttaaggcc taaattcgga aaatcaacca
    31081 gcgttaactt tcttgttctc gttgtgcaat ttcgcgattt tcttccgcag gtggtgttaa
    31141 tggcccacaa attcgttgcg caagccacgt tgccaccgcc accagcgccc acgaaatcaa
    31201 cgtagctacc accagatcgc gtggccaatg catcccgagc agcaggcggc ttcccatgac
    31261 tcccgttgcc cagaccaaca agatagcaat ggttaacgtt cgccgacgcg gccacagcaa
    31321 accaacggcc agcagtgccc aactggcagc aaacatcgtg tgaccggaag gaaaggcaaa
    31381 ccccgtctct ttctgccagt gtgaacgcaa atattgtgga atatttttct cttcagccaa
    31441 ctgttctttt actagatttc cgcgttctgc tcgctttaaa gtgtagaact catcaaccgg
    31501 aatatgatgt gttttttcca accagataac aaaaggtcgt ggttcctgta ctttgtcttt
    31561 gatccaggat ttaacgcctt gtccaacaag gattgccgcc gccagaatgg caaataatac
    31621 aaaggcagcc ttaatgcgaa aacgcagaca ccagagaaac cagccgaaca aaatcaaatg
    31681 tgtaatgacg ccccagggct gggtgacagt ttcagtaacc caaaaagccg cttttagtag
    31741 ccaactttgt tctccaggtt gccaacgcca gccagaaatc catacggcta ctggcatgac
    31801 aagcaatagt gcagctccca ctgcggtacg tcgggcaatc gaacgcatgg cctctccttt
    31861 ttgataagtc ccacaatcat aactgaaaat gccagttcca agaaaaattg acagatttgt
    31921 gccattccgt gaacgatcga cgcgtcgtga ttaggtgaac cccttctcgt tatggcaaaa
    31981 taagccaata cagaaccagc attatctgga gaatttcatg cagcttaaac gtgtggcaga
    32041 agccaaactg ccaaccccat ggggcgattt cctgatggtg ggatttgaag aactggcaac
    32101 cggacacgat catgttgcgc tagtctatgg cgatatttcc gggcataccc cggtacttgc
    32161 gcgcgtccat tccgaatgtc tgaccggtga cgccctgttc agcttgcgct gcgattgtgg
    32221 cttccagctc gaagcggcat tgacgcaaat tgccgaggaa ggccgtggta ttttgctgta
    32281 tcaccgtcag gaaggtcgta acattggtct gctgaataaa atccgcgctt acgcactgca
    32341 ggatcaaggt tacgataccg tagaggctaa ccaccagtta ggcttcgccg ctgatgagcg
    32401 cgacttcact ctttgcgctg atatgttcaa actccttggc gtcaatgaag tccgcttgtt
    32461 aaccaataac ccgaaaaaag tcgaaattct gaccgaagca gggattaata ttgttgaacg
    32521 cgtaccattg attgtaggtc gtaaccccaa taacgaacat tatctcgata ccaaagccga
    32581 gaaaatgggc catttgctga acaaataacc ctcttgcatc gtgtaattca tttgcttgcc
    32641 ggaagcaaaa taaccggcaa gcaaatactt gttacttcaa catattacga atgacataat
    32701 gcaaaatgcc gtcgttctgg tagtaggtca actccgtcgc ggtgtcgata cgacaacggc
    32761 agggtacgac ttcctggcta ccatccgcgc gcgtaagcgt caccggaacc gtcgcgccag
    32821 gttgtagatt ttgcagatcg ccaatatcaa tcttctcttc cccggttagc cctaacgttt
    32881 tacgcgtcac gccttgcgga aattccagcg gcaggatgcc catgccaatt aaattcgaac
    32941 ggtgaattcg ttcaaacgat tcggcaatca ccacacgaat tccaagcaga cgaggtcctt
    33001 ttgccgccca gtcacggctt gagcctgatc catactcttt cccggcaatc accgccagcg
    33061 gcgtttgctc ctgcttatag cgcatcgcag catcataaat agagactacg tcgctgtcag
    33121 gtaaatgccg cgtcatcccc ccttcaacgc caggcaccat ttcattacgg atgcgaatat
    33181 tggcgaaggt gccgcgcatc atcacttcat ggttaccacg ccgcgaaccg taggagttaa
    33241 agtcttttcg ctcaacaccc cgaccttgta gatatcgacc cgctgggctg tcgggcttaa
    33301 tactgcccgc cggagagata tggtcagtgg tgactgaatc ccccagcatt gcgaggatcc
    33361 gcgcaccgtg aatatcttcc actggtgctg gtgttgcctg catttcatca aagaaaggcg
    33421 ataagcgaat ataggttgag tcctcctgcc aaccgtaggt atcggatcgt gtgacgttaa
    33481 tttccttcca ctctgctgtg ccttcaaaaa cttctgcgta ctctttgcgg aacatttctg
    33541 tggagacttg ttctaccgca cgggcaattt cttgtgccga tggccagata tctttcagat
    33601 aaaccggctc gcctttgcga tcatggccga taggctcaga agccaggttg atattcatat
    33661 ttcccgccag cgcataggca accaccagcg gtggcgaggc cagccagtta gttttaacca
    33721 gcggatggat acggccttca aagttacggt tgccggacag caccgcaccg acggttaaat
    33781 cgcctttttt gattgccgtt tcgataggat cgggcagcgg cccagagtta ccaatacagg
    33841 tggtacaacc gtatcccaca aggttaaacc ccagttcgtc gagatacggt gtcagtttcg
    33901 cttttgccag ataatcagaa acgactttcg aacccggtgc caacgacgct ttgacccacg
    33961 gttgccgctt gaggccaaga gttacggctt ttttcgccag caagcctgcg gccatcagca
    34021 cacttgggtt agaggtgttg gtgcacgagg ttatcgcagc aatgaccaca gcgccatcag
    34081 gtaactgata ctgatgtccg ttcataacat aatcgactgg caggcgatct ttatgcgtgg
    34141 cattcacttt cagttcgtta ctggcggcaa atgcttttgg tacatcgggc agtgcaacgc
    34201 gatcctgtgg gcgttttggc cctgccaggc tcgcttcaac gtcattcata tccagttcta
    34261 acatactggt aaaaatcggt tcatcgcccg ggttacgcca catgccctgc gctttggcat
    34321 atttttcgac caactcgacc tgatcttcgc tgcgcccgct taaacgcatg taatcgaggg
    34381 ttactgcatc gattgggaag aagccacagg tggcaccata ttctggcgac atattggcaa
    34441 tggtggcgcg atccgccaac ggtagtgaat ccagaccatc accataaaat tcgacgaatt
    34501 tccccaccac gccatgtttg cgcagcattt gggtaacagt gagaaccagg tctgtggcgg
    34561 taataccttc acgtaatttt ccggtaagtt taaagcccac tacgtccggg ataagcatgg
    34621 aaaccggctg gcctaacatg gcggcttctg cttcgatccc acccacgccc caccccagca
    34681 cgccaaggcc gttgatcata gtggtgtgcg agtcagtacc aacgagtgta tccggataag
    34741 caatccattc accgtcctgc aattcgctcc acactgcttt gccgagatat tcgaggttaa
    34801 cctgatggca aatgcctgtg cctggcggca cgacgctaaa ccgactgaac gcttgctttc
    34861 cccatttcag gaacacataa cgttcgtggt tgcgctccat ttccaggcgt acgttttctt
    34921 caaatgcctc atcatcacca aaacgatcga cggtcaccga gtggtcaatg accaggtcga
    34981 ccggtgagag cgggttaacc tttgcagtat cgccaccgag gcgtttaacc gcttcgcgca
    35041 ttgccgccag atcaacaacg gcaggtacgc cggtaaagtc ctgcatcagc acccttgccg
    35101 ggcggtaggc aatttcacgg tcagcatggg catttttcag ccatcccgcc agtgcgtgga
    35161 tatcctcttc ggtaactgag ttaccatcct gccagcgcag caggttttcg agcaaaactt
    35221 tgagtgactt gggtagacgg gtgatatcgc ccagtgattt agcagcaagc ggcaggctgt
    35281 agtagtggta agttttatct ttggcctgca acgtgtcctt actggcttct cgtagggttg
    35341 acgacatagc tcctccttaa tgacagggtt gcgaagccct gattctcttc agggtgaata
    35401 ttaaagataa cacaaacatc gcgtaacgat ttgataacaa cccaaattga taaaagagga
    35461 gaaaccttgt ggcaaagcaa atgacaaccc cgccgcagcg gggtcaagga tcagtgagta
    35521 atgatccaga ggagttcggc ccagaagagc atcgacagag aaaacgcgcc catccaggac
    35581 caatatttca tatttgtgtt gatcataggc tacaccctta acggttatta ttttgtgttg
    35641 agataactca agctgttatt gctgcggtgt attaaaagcc ctttaattca ttaaaaatgg
    35701 gtaataagaa tgatgataat tattgtttcg gctgatcatc ttcagcaaac agttctataa
    35761 aggcttgctg ctgcgatgtt agttgccagt cgtctggaat aacgacggaa cattcccgtt
    35821 tcgtttctgg ttcattgtca ctatgctggc ataatggtga cgactccagt tgctcctgac
    35881 cgaccatagt tatccccaca ctttccaaat cagcagtgcg acaaccaccc agaaaagtgc
    35941 agaaccaaga aaaaccgcca gccaggcttt acgttttaat tcaggatcac ggcggggttc
    36001 ctgatttcca gaaggcattg ctaacctcat ataatcgcca tcacttatca gcaagacgtt
    36061 gaatgattgg tgtaattaat catacgatga gacaaatcct aaagaaactt tagtcaaaaa
    36121 tccagcgaat ttacgaatcg tctccagtga ataattcaat cttttgacct gaaatagata
    36181 tttgaaaact ggaatttgcg aggcgggtaa ttagacacta ctttacaggt aatttaccct
    36241 ataaatatga gggataaaaa tattgtgcac ggtatagaaa taagtaacaa aagagaatat
    36301 attccggcac cttcgctaca taaaaggtgc cgaaaataac gcaagaaatt atttttccgg
    36361 cagttttata tctttaaaca tgacctcaat ttcttcattg gagcgcaatg cgacagccgc
    36421 atcaacgaca tcacgcgtta aatgcggtgc aaaacgctga atgaaatcat acatataact
    36481 gcgcaaaaaa gtactacggc gaaaaccaat tttggttgta ctgtggctga agatatcgtg
    36541 agcatcaacg cgcacaaggt cgggatcggc gaccggatcc accgccatgc tggcaatgac
    36601 ccctaccccc agccctaacc ggacgtaagt tttaatgacg tcagcatccg ttgccgtgaa
    36661 aacgatacgc ggcgttaacc ctgcgcggtt aaaggcagta tccagttctg aacgtccggt
    36721 aaagccgaag gtatatgtca ccaacggata ttgcgccagt tcttcaatgg taatggcttt
    36781 tttgcctgcc agcgggtgat ccggagtgac tacaatagcc cgattccagt ggtagcacgg
    36841 taacatcact aaatcttcat acagatgcag cgcttctgtg gcgatagcga aatcggcatt
    36901 gcctttagag acggcatcag caatttgtgt cggcgggccc tggtgcatat gcaaagaaac
    36961 gcggggataa cgctcaataa agcctttgat aacgtttggt aacgcgtagc gtgcctgggt
    37021 atgcgtggtg gcgatataca gcgagccttt atccggccag gtgtgctcac cggcaaccga
    37081 ttttatggca tcgactttcg acaggacttc gcgagcgata cgaattattt cttgtcctgc
    37141 tggcgttacc tgcgtcaggt gcttgccgct tcgggaaaaa atttgaatgc ctagctcgtc
    37201 ttccagcatt ctgacttgtt tactgatccc gggttgtgat gtgtaaagtc cttccgctgt
    37261 tgatgagaca ttcaggttat gattgaccac ctcaacaata tagcgaagtt gttgtaattt
    37321 catgttaaac catccactta gacgccatca gaacatcgtt ttttcgtcac acctggcggg
    37381 tgactaatac gatttaataa taaaaaaggt gctaactata accactatat catttatagc
    37441 cttagtgtat aggtgtgaag aaaaaataat atgcaataaa aaagggccgc tttcgcgacc
    37501 ctttgtttat aaaaacctga cagaattaaa ggttattttt ttccttcaac ccatttgcca
    37561 tcaacataaa atgctgacca gccagtcgcc tttccgtctt tttccgaaga gacatattgc
    37621 tgtttggttt tacggctaaa gcgaaccatg gtcttattac cttccggatc ctgctgtggc
    37681 gcatcggcca gataacgcag tttttccggc agacggtcgc ggaagcgata aagctcttcc
    37741 accagtggcg cacgcgtttc acgcgatttc gggaaagtgt tggcagccag gaacacaccg
    37801 gcagcaccgt cacgcagcac gaaataagca tctgattttt cgcacggcag ctcaggtaat
    37861 ggcaccggat cttctttcgg tggtgccact tcaccgttac gtaaaatctt acgtgtgttt
    37921 ttacactctt cgttggtgca ggccatgtat ttaccgaaac gccccatttt caggtgcatt
    37981 tcagagccac atttttcaca ctcaacgatc gggccgtcat aacctttaat gcggaattcg
    38041 ccctcttcga tctcgtaacc gtcgcaggtt gggttattac cacagacatg caacttacgt
    38101 ttcggatcga tgagatagct gtccatcgcc gtgccgcatt tcgggcaacg acgttttgcg
    38161 cgcagcgcgt tggtttcagc atcttcgcct tccagcacgt tcagcacttc gttttccggc
    38221 accaggttaa tggtggtttt gcaacgctct ttcggcggca gcgcatagcc agaacagcca
    38281 aggaataccc cggtactcgc tgtgcgaatc cccattttgc gaccacaagt cgggcagtca
    38341 atgctggtca gaaccatctg gttcgggcgc ataccaccct cttccggatc tttttcagct
    38401 ttatctaact gctgggtgaa atccgagaag aagtgatcca gtacagcttt ccactctgct
    38461 tcgtgatttg ccacctggtc gaggctgttt tccatctgcg cggtaaagtc gtagttcatt
    38521 aactcgcgga aattttcttc aaggcgatcg gtgacgattt cgcccatttt ttccgcatag
    38581 aaacgacgat tttctactcg cacatagcca cgatcctgaa tggtcgaaat gatcgacgca
    38641 taggtagacg gacgaccgat accacgtttt tccagttctt taaccagcga tgcttcactg
    38701 aaacgggctg gcggcttggt aaagtgctgg gctggtgtta gttcaacgag cgtcagagca
    38761 tcgcctttat cgactgccgg taagatacga tcttcatcgc ctttacgcag tgcaggcatc
    38821 acttttgtcc agccatcaaa gcgcaaaata cgaccgcgtg ctttcaggcg gaaatcgccc
    38881 gcaccaacgg tcagcgtcgt ggagtcatat ttcgctgggg tcatctggca ggcaacgaac
    38941 tgacgccaga ttaactggta cagtttctgc gcatctgctt ccatatcctt cagcgattcc
    39001 gccatcacat tgacgtcaga aggacgaatc gcttcgtgcg cttcctgtga gttttctttg
    39061 ctggcgtact gattcggact ttccggcaga tatttcttac caaaattatc gctgatataa
    39121 ccgcgaacca tatttaccgc gtcctgactc aggttagtgg agtcggtacg catgtaagtg
    39181 atatagcctg cttcatacaa acgctgcgcc atcatcatgg tttttttcac gccaaaacca
    39241 agacgggtgc tggcagcttg ttgcagcgta gaggtaataa aaggagcgcc aggtttactg
    39301 gttgtcggtt tgtcttcacg ttccagcacg ctgtagcgcg ctttttccag cagacttacc
    39361 gcagcctgag tttgttcttt gttgaccgga cggaacggtt tgtcattctg atgagtcacc
    39421 tgcaacgcca acgcttcacc agatggcgta gtcgtgctgg catcgacttc ccagaactct
    39481 tccggtacga acgctttaat ttcacgctca cgctctacca ccaggcggac cgccaccgac
    39541 tgcacacgac cggcagacag accacgagcg atctttttcc acagcagcgg cgaaaccata
    39601 taccccacca cgcggtccat aaagcgacgc gcctgctggg cattaacacg atcaatattc
    39661 agctcacccg gtttgttaaa tgcctggcgg atcgcgtttt tagtaatttc gttaaacacc
    39721 actcggctat agcgcgcatc atcaccacca atcacttccc gcaggtgcca tgcaatggct
    39781 tccccttcgc ggtcaaggtc ggttgcgaga tagatgtggt cggctttttc agccagttgt
    39841 ttcagttcag agacgacctt ctctttacca ggcaacactt catagtgcgc ctcccaattg
    39901 tgccacgggt caacccccat acggttgacg agagcgccac gttcatcctt tttaggcttt
    39961 ttagccgtct tggtggaggt agagtcggca ctctttttgg cagctgagcc actggtcggc
    40021 aaatcgcgga tgtgaccgac gctggatttc accacgtagt cacaacccag atacttgttg
    40081 atcgttttgg cttttgccgg ggactcaacg atgacaagag ctttacccat attcacctta
    40141 cctaatttga ttcttccagg aatgcgtcgc acgttgataa accttccact ggcaacgagt
    40201 taccgatatg gactcgctct ctgcggatat caaccccttg tcgtgactct gttcacgaaa
    40261 attcaggtga ctgagttaac aggtcttggc ctacaggcgc tatagcacga cggaattttg
    40321 gtcgaatgtc aagcaaatct attgccagat tgacgaaagc gtcacactgt acctgataaa
    40381 atccgatacg caactttatt agcatgcaaa atccaaagtt gccagtaagg cgctcttgcc
    40441 accagcgtgg tatatttgtc tgcaagccat gcctggcgta gactaatgtc cttgtttaag
    40501 gagtaaataa tgaacaaaga gacacaaccc atcgaccgcg aaacgctgtt gaaagaggcc
    40561 aacaaaatca ttcgtgagca tgaggatacg ctcgcaggga ttgaagccac cggcgtcacg
    40621 cagcgtaatg gcgtgctggt atttaccggc gactactttt tagatgaaca aggattgcca
    40681 acagccaaaa gcaccgcggt gtttaatatg ttcaaacacc tggcccatgt gctttcagaa
    40741 aaatatcatc tggttgatta aagcaaaacg cgaggtctta gcctcgcgtt tgtcttttac
    40801 atcaatggct tttgaccccg ctgccaccag cgtagcaaca atcgatcggc gctctctgcc
    40861 gcgctgccgg tgaatcggtc aatgagtcgt ttacgctgca tatagcgtac attaaccact
    40921 tcacggcctt ccatcaggct aagaataact tcatcactgg tgttgatttc atcaaccagg
    40981 cctttctcta ccgcctgttg tccgtaccag tgttcacccg ttgccacctg ttcaatatcc
    41041 agagacggac gcatacgctt cacaaaatct ttaaataact gatgcgtttc gttcagctct
    41101 tcgcggaatt tctcccgccc ttcttcggta ttttcaccca gcaaggtcag cgtgcgctta
    41161 tactgcccgg cggtgtgcag ttcgatatca atatctttgc ttttcaggaa gcggttaaag
    41221 ttgggcattt gcgccaccac cccaatggaa cccacaatag caaacggtgc ggaaacaatt
    41281 ttgtccgcca cacaggccat catgtaaccg ccgctggcag cgactttgtc taccgtaaca
    41341 gttaaaggaa tgtttttatc acgcagacgc tgcagctgcg aagccgccaa cccgtaacca
    41401 tgcaccatgc caccagggct ttccagacgg agcacaacct gatcctgcgg tttgaatgct
    41461 gcgagtaccg ccgttatctc ttcacgtagc gagttcactt catgggcgtc catgctgcct
    41521 ttaaaatcca gcacccagac gcgaggttta ctgtcagttg ccacctcgcc cagtttggct
    41581 ttcgcttttg ctgctttcgc ttcttgcttg tgctttttct tctgcgcttt gtgccactgt
    41641 ttttgctgat gtgagtccat cagcgccgcg gccagttctt ctttcatctc cttatactgt
    41701 tcgctgagat tgttgacccg taactcgcca cgctggcgtt tattacgttg agcaacattg
    41761 acaataatgg cggcaatcgc cgcaatcgct agcacaacgg taacgatttt agccaaaaac
    41821 aaaccatatt cagacaacaa ttccacgagt ccccaccttg gttaaacaac gctttggctc
    41881 acagtttaca acaggcatgt taagccgtct cgcagagatg ttattgcagc gaaagtatga
    41941 caaagtatcc attttgcggt gttatcgccc caggctgatt gaaatgccct tctgtttcag
    42001 gcataaagcc ccaaagtcat aaagtacact ggcagcgcga cagacaacgt cgttgaggag
    42061 tagccatgca ttaccagcca aaacaagatt tactcaatga tcgcattatc ctggtgacgg
    42121 gagccagcga tgggattggt cgtgaagccg cgatgacgta tgcacgctat ggtgcgacag
    42181 tgattctgtt gggccgtaat gaagaaaaat tacgtcaggt agccagccac ataaacgaag
    42241 aaactgggcg tcagccacag tggtttattc tcgatttgct gacctgcacg tccgaaaatt
    42301 gccaacaact ggcacagcgc attgtcgtta attatccgcg tctggatggt gttttgcata
    42361 atgccggatt gctcggcgat gtttgcccaa tgagcgaaca aaatccgcag gtctggcagg
    42421 acgtcatgca gatcaacgtt aatgccacct ttatgctcac ccaggcactg cttcctttat
    42481 tactcaaatc ggacgccggt tcactggtct ttacttcatc aagcgttgga cgtcagggac
    42541 gagccaactg gggtgcatat gcagcgtcga aatttgccac cgaagggatg atgcaggtac
    42601 tggccgatga atatcagcag cgcctgcgcg tcaactgcat taacccaggc ggtacgcgca
    42661 ccgcaatgcg cgcaagcgcc ttcccgaccg aagatccaca gaaacttaaa acacccgctg
    42721 atatcatgcc gctctacctc tggctgatgg gcgatgacag ccgccgtaaa accggcatga
    42781 cctttgacgc ccaaccgggc cgtaaaccag gaatttccca atgagtgatg aacgctacca
    42841 acagcgtcag cagcgagtga aagaaaaagt agatgctcgt gtggcccagg cccaggatga
    42901 acgcggtatt atcatcgtct ttaccggcaa tggaaaaggc aaaaccaccg cggcatttgg
    42961 tacggcaaca cgcgcagttg gtcacggaaa aaaagtgggc gtcgtgcagt ttattaaagg
    43021 cacctggcct aatggcgaac gcaatctgct ggagccacat ggcgttgagt ttcaggtgat
    43081 ggcaacgggc tttacctggg atacacaaaa ccgcgagtct gataccgccg cctgccgcga
    43141 agtctggcaa catgcaaagc ggatgcttgc tgattcctca ctggatatgg ttttgcttga
    43201 tgaactgacg tatatggtgg cgtatgacta tttgccactg gaagaagtgg tgcaggcgtt
    43261 aaatgaacgt ccacatcaac agacggtgat tatcacgggt cgaggctgtc atcgggatat
    43321 tcttgaactg gcggatacgg taagtgagct gcgccccatc agacatgcgt ttgatgccgg
    43381 tgttaaggcg caaataggga ttgattatta atctgtaaaa atgccggata tgacgtccgg
    43441 catactttga ttaaccgtta ttacgcccgc cagaacgacg actgccgctc acctgactgt
    43501 gacgtttcac cgcacgacga atctgattag ccttcatgcg acgacggtct ttttctacag
    43561 cgactttaga gctggtttct ggcggaagct ccaccagttc gcgcagatag ttagtctggg
    43621 cgaggtccag ctccgtccag ccaccgcgtg gcagaccttt tggcagtggg atatcaccgt
    43681 aacgaacacg gatcaggcgg ctcacctgca caccaaccgc ttcccacaga cgacgaactt
    43741 cacggttacg gccttcggtc agagtcacgt tgtaccactg gttgatccct tcgccgccgc
    43801 tgaacttgat ggttttaaaa gctgccggac catcttccag ttgcacgcca cgactcaaat
    43861 cacgcagttt cgcgtcgtca acctgaccaa atacacgcac ggcgtattca cgttcaactt
    43921 cacggcttgg atgcattaaa cggtttgcca gttcaccatc ggtggtgaac agcagcagac
    43981 cacaggtgtt aacgtccaga cgacctacgg caatccagcg tgcgccacgc agctttggca
    44041 gacggtcaaa caccgtcgga cgcccttccg gatcgttacg ggtacacaac tcaccttccg
    44101 gtttgtaata ggccagaacg cgacaaattt gttcagcgga ctcacgtacc gaaatcagat
    44161 gaccatcgat acgaattttc agcccagggg taacttcaac gcgatcgccg agtttagcaa
    44221 ttttgccatc aacactcaca cgaccggctt caataataga ttcgatttca cggcgggagc
    44281 cgtggccagc gcgggccagc actttctgta atttttcgct catagagctt ccttttggtg
    44341 tcgccttcac aggcgtcgaa caggagaatc aatggcgccg tatagcgcca ttttaaggcc
    44401 gcgtagtata cagagttacg cacttataag aaaggcttca catcacctac gccttcacgc
    44461 accacgaccg gcgtatcatc ggtaagatca ataaccgttg ttggtttctg cccgagatag
    44521 ccgccgtgaa taatcaaatc tacctgtttt tccagacggt ctttaatttc ttcgggatcg
    44581 gattcggtaa attcgctgcc tggcagcatt agcgaagtgg aaagcatcgg ttcgcccagt
    44641 gcttcaagca acgcctgcgc aatcgggtta gaaggcacac gcataccgat ggttttgcgt
    44701 ttctcctgca acagacggcg tggcacttcc ttcgtcccct tcaggatgaa ggtatagttg
    44761 cccggcgtgt tgtttttcat caaacgaaac gcaacgttat ccacgaatga ataggtcgag
    44821 agttcagaaa gatcgcgaca catcagggta aagttgtgac cgtccggcag ctggcgaata
    44881 cgacaaatac gctccatggc gtttttatct tcaattttac agccgagcgc atagccggaa
    44941 tcagttggat agacaatcac ccctccttta cgcacgatct ccaccgcctg gttgatcaga
    45001 cgttgctgcg ggttatcagg atgaatataa aaaaactggc tcataattcc ctctctgtgg
    45061 tgttctgcgg ctgttcccat agctgccaga cgccttcaac gcctgcgggc agccagagtt
    45121 tacgacccag ttcgatccac gggcatggct gatgaaaatc agatccttgt gatgcccata
    45181 aatgatgctg acgcgcaagt gtggccagct gggtgcgttc attgggcgat tgctggcact
    45241 gcgcgacttc catcgcgtcg ccgtggtgtt cggcaaaatg cgcaaccaat cttttcagcc
    45301 atttagcact aagattgtac cgcccaggat gagctagcac cgccttaccg ccagaatgat
    45361 gaatgacatc aatagcttgt tctattgtac accactgtgg cggaacgtat ccggttttcc
    45421 cgcgcgccag atacttttta aagacatctg ccattgaact ggctttgccg cactccacaa
    45481 ggaaacgcgc aaaatgaccg cgcgtcactg cgccgccctg cgccagtcgt tgtgcgcctt
    45541 ccagcgcccc aggtatttgc gctttttcaa gtctttcggc aatttgctgc gcccgctgat
    45601 tgcgccgttc tgtctgctgc gcgaggaact cacacattag cggatgagta atatcaatat
    45661 tcagcccgac aatatgaatt tcatgatttt cccagaccgt ggaaatttcc acgccgggaa
    45721 taagattcag cgccagtccc gaacgtgaaa tttcctctct tgctggcgcg attgcagctg
    45781 tggtgtcatg gtcggtgatc gccagggtgc cgacgcgcat ctcgactgca cggtgcacca
    45841 atgcttctgg cgtcaggcag ccatcggaag ctgtggtatg gctgtgcagg tcgtaaatca
    45901 ctgcataatt cgtgtcgctc aaggcgcact cccgttctgg ataatgtttt ttgcgccgac
    45961 atcataacgg gtctggcaaa tattctgaaa tgagctgttg acaattaatc atcgaaccag
    46021 ttaactagta cgcaagttca cgtaaaaagg gtatcgacaa tgaaagcaat tttcgtactg
    46081 aaaggttggt ggcgcacttc ctgaaacggg cagtgtattc accatgcgta aagcaatcag
    46141 atacccagcc cgcctaatga gcgggctttt ttttgaacaa aattagagaa taacaatgca
    46201 aacacaaaaa ccgactctcg aactgctaac ctgcgaaggc gcttatcgcg acaacccgac
    46261 tgcgcttttt caccagttgt gtgggaatcg tccggcaacg ctgctgctgg aatccgcaga
    46321 tatcgacagc aaagatgatt taaaaagcct gctgctgata gacagtgcgc tgcgcattac
    46381 agcattaggt gacaccgtca caattcaggc actttccggc aatggcgaag ccctgctggc
    46441 actactggat aacgccctgc ctgcgggtgt ggaaagtgaa caatcaccaa actgccgtgt
    46501 gctgcgcttc ccccctgtca gtccactgct ggatgaagac gcccgcttat gctccctttc
    46561 ggtttttgac gctttccgtt tattgcagaa tctgttgaat gtaccgaagg aagaacgaga
    46621 agcaatgttc ttcggcggcc tgttctctta tgaccttgtg gcgggatttg aagatttacc
    46681 gcaactgtca gcggaaaata actgccctga tttctgtttt tatctcgctg aaacgctgat
    46741 ggtgattgac catcagaaaa aaagcacccg tattcaggcc agcctgtttg ctccgaatga
    46801 agaagaaaaa caacgtctca ctgctcgcct gaacgaacta cgtcagcaac tgaccgaagc
    46861 cgcgccgccg ctgccagtgg tttccgtgcc gcatatgcgt tgtgaatgta atcagagcga
    46921 tgaagagttc ggtggcgtag tgcgtttgtt gcaaaaagcg attcgcgctg gagaaatttt
    46981 ccaggtggtg ccatctcgcc gtttctctct gccctgcccg tcaccgctgg cggcctatta
    47041 cgtgctgaaa aagagtaatc ccagcccgta catgtttttt atgcaggata atgatttcac
    47101 cctatttggc gcgtcgccgg aaagctcgct caagtatgat gccaccagcc gccagattga
    47161 gatctacccg attgccggaa cacgcccacg cggtcgtcgc gccgatggtt cactggacag
    47221 agatctcgac agccgtattg aactggaaat gcgtactgac cataaagagc tgtctgaaca
    47281 tctgatgctg gttgatctcg cccgtaatga tctggcacgc atttgcaccc ctggcagccg
    47341 ctacatcgcc gatctcacca aagttgaccg ttattcctat gtgatgcacc tcgtctctcg
    47401 cgtagtcggc gaactgcgtc acgatcttga cgccctgcac gcttatcgcg cctgtatgaa
    47461 tatggggacg ttaagcggtg cgccgaaagt acgcgctatg cagttaattg ccgaggcgga
    47521 aggtcgtcgc cgcggcagct acggcggcgc ggtaggttat ttcaccgcgc atggcgatct
    47581 cgacacctgc attgtgatcc gctcggcgct ggtggaaaac ggtatcgcca ccgtgcaagc
    47641 gggtgctggt gtagtccttg attctgttcc gcagtcggaa gccgacgaaa cccgtaacaa
    47701 agcccgcgct gtactgcgcg ctattgccac cgcgcatcat gcacaggaga ctttctgatg
    47761 gctgacattc tgctgctcga taatatcgac tcttttacgt acaacctggc agatcagttg
    47821 cgcagcaatg gtcataacgt ggtgatttac cgcaaccata ttccggcgca gaccttaatt
    47881 gaacgcctgg cgacgatgag caatccggta ctgatgcttt ctcctggccc cggtgtgccg
    47941 agcgaagccg gttgtatgcc ggaactcctc acccgcttgc gtggcaagct gcccattatt
    48001 ggcatttgcc tcggacatca ggcaattgtc gaagcttacg ggggctatgt cggtcaggcg
    48061 ggcgaaattc ttcacggtaa agcgtcgagc attgaacatg acggtcaggc gatgtttgcc
    48121 ggattaacaa acccgctgcc ggtggcgcgt tatcactcgc tggttggcag taacattccg
    48181 gccggtttaa ccatcaacgc ccattttaat ggcatggtga tggcagtacg tcacgatgcg
    48241 gatcgcgttt gtggattcca gttccatccg gaatccattc tcaccaccca gggcgctcgc
    48301 ctgctggaac aaacgctggc ctgggcgcag cagaaactag agccagccaa cacgctgcaa
    48361 ccgattctgg aaaaactgta tcaggcgcag acgcttagcc aacaagaaag ccaccagctg
    48421 ttttcagcgg tggtgcgtgg cgagctgaag ccggaacaac tggcggcggc gctggtgagc
    48481 atgaaaattc gcggtgagca cccgaacgag atcgccgggg cagcaaccgc gctactggaa
    48541 aacgcagcgc cgttcccgcg cccggattat ctgtttgctg atatcgtcgg tactggcggt
    48601 gacggcagca acagtatcaa tatttctacc gccagtgcgt ttgtcgccgc ggcctgtggg
    48661 ctgaaagtgg cgaaacacgg taaccgcagc gtctccagta aatccggctc gtcggatctg
    48721 ctggcggcat tcggtattaa tcttgatatg aacgccaata aatcgcgcca ggcgctggac
    48781 gagttaggcg tatgtttcct ctttgcgccg aaatatcaca ccggattccg ccacgcgatg
    48841 ccggttcgcc agcaactgaa aacccgcacc ctgttcaatg tgctggggcc attgattaac
    48901 ccggcgcatc cgccgctggc gttaattggt gtttatagtc cggaactggt gctgccgatt
    48961 gccgaaacct tgcgcgtgct ggggtatcaa cgcgcggcgg tggtgcacag cggcgggatg
    49021 gatgaagttt cattacacgc gccgacaatc gttgccgagc tgcatgacgg caaaattaag
    49081 agctatcaat tgaccgctga agattttggc ctgactccct accaccagga gcaactggca
    49141 ggcggaacac ctgaagaaaa ccgtgacatt ttaacacgct tgttacaagg taaaggcgac
    49201 gccgcccatg aagcagccgt cgctgcgaac gtcgccatgt taatgcgcct gcatggccat
    49261 gaagatctgc aagccaatgc gcaaaccgtt cttgaggtac tgcgcagtgg ttccgcttac
    49321 gacagagtta ccgcgctggc ggcacgaggg taaatgatgc aaaccgtttt agcgaaaatc
    49381 gtcgcagaca aggcgatttg ggtagaaacc tgcaaacagc agcaaccgct ggccagtttt
    49441 cagaatgagg ttcagccgag cacacgacat ttttatgatg cgctacaggg tgcacgcacg
    49501 gcgtttattc tggagtgtaa aaaagcgtcg ccgtcaaaag gcgtgatccg tgatgatttc
    49561 gatccggcac gcattgccgc catttataaa cattacgctt cggcaatttc ggtgctgact
    49621 gatgagaaat attttcaggg gagctttgat ttcctcctca tcgtcagcca aatcgccccg
    49681 cagccgattt tatgtaaaga cttcattatc gacccttacc agatctatct ggcgcgctat
    49741 taccaggccg atgcctgctt attaatgctt tcagtactgg atgacgacca atatcgccag
    49801 cttgcagccg tcgctcacag tctggagatg ggggtgctga ccgaagtcag taatgaagag
    49861 gaactggagc gcgccattgc attaggggca aaggtcgttg gcatcaacaa ccgcgatctg
    49921 cgtgatttgt cgattgatct caaccgtacc cgcgagcttg cgccgaaact ggggcacaac
    49981 gtaacggtaa tcagcgaatc cggcatcaat acttacgctc aggtgcgcga gttaagccac
    50041 ttcgctaacg gttttctgat tggttcggcg ttgatggccc atgacgattt gcacgccgcc
    50101 gtgcgccggg tgttgctggg tgagaataaa gtatgtggcc tgacgcgtgg gcaagatgct
    50161 aaagcagctt atgacgcggg cgcgatttac ggtgggttga tttttgttgc gacatcaccg
    50221 cgttgcgtca acgttgaaca ggcgcaggaa gtgatggctg cggcaccgtt gcagtatgtt
    50281 ggcgtgttcc gcaatcacga tattgccgat gtggtggaca aagctaaggt gttatcgctg
    50341 gcggcagtgc aactgcatgg caatgaagat cagctgtata tcgatacgct gcgtgaagct
    50401 ctgccagcac atgttgccat ctggaaagca ttaagcgtcg gtgaaaccct gcccgcccgc
    50461 gagtttcagc acgttgataa atatgtttta gacaacggcc agggtggaag cgggcaacgt
    50521 tttgactggt cactattaaa tggtcaatcg cttggcaacg ttctgctggc ggggggctta
    50581 ggcgcagata actgcgtgga agcggcacaa accggctgcg ccggacttga ttttaattct
    50641 gctgtagagt cgcaaccggg catcaaagac gcacgtcttt tggcctcggt tttccagacg
    50701 ctgcgcgcat attaaggaaa ggaacaatga caacattact taacccctat tttggtgagt
    50761 ttggcggcat gtacgtgcca caaatcctga tgcctgctct gcgccagctg gaagaagctt
    50821 ttgtcagcgc gcaaaaagat cctgaatttc aagcccagtt caacgacctg ctgaaaaact
    50881 atgccgggcg tccaaccgca ctgaccaaat gccagaacat tacagccggg acgaacacca
    50941 cgctgtatct caagcgtgaa gatttgctgc acggcggcgc acataaaacc aaccaggtgc
    51001 tggggcaggc gttgctggcg aagcggatgg gtaaaaccga aatcatcgcc gaaaccgggg
    51061 ccggtcagca tggcgtggcg tcggcccttg ccagcgccct gctcggcctg aaatgccgta
    51121 tttatatggg tgccaaagac gttgaacgcc agtcgcctaa cgtttttcgt atgcgcttaa
    51181 tgggtgcgga agtgatcccg gtacatagcg gttccgccac gctgaaagat gcctgtaatg
    51241 aggcgctacg cgactggtcc ggcagttatg aaaccgcgca ctatatgctg ggcaccgcgg
    51301 ctggcccgca tccttatccg accattgtcc gtgagtttca gcggatgatt ggcgaagaaa
    51361 ccaaagcgca gattctggaa agagaaggtc gcctgccgga tgccgttatc gcctgtgttg
    51421 gcggcggttc gaatgccatc ggcatgtttg ctgatttcat caatgaaacc aacgtcggcc
    51481 tgattggtgt ggagcctggt ggtcacggta tcgaaactgg cgagcacggc gcaccgctaa
    51541 aacatggtcg cgtgggcatt tatttcggta tgaaagcgcc gatgatgcaa accgaagacg
    51601 ggcagattga agaatcttac tccatctccg ccggactgga tttcccgtct gtcggtccac
    51661 aacacgcgta tcttaacagc actggacgcg ctgattacgt gtctattacc gatgatgaag
    51721 cccttgaagc cttcaaaacg ctgtgcctgc acgaagggat catcccggcg ctggaatcct
    51781 cccacgccct ggcccatgcg ctgaaaatga tgcgcgaaaa cccggataaa gagcagctac
    51841 tggtggttaa cctttccggt cgcggcgata aagacatctt caccgttcac gatattttga
    51901 aagcacgagg ggaaatctga tggaacgcta cgaatctctg tttgcccagt tgaaggagcg
    51961 caaagaaggc gcattcgttc ctttcgtcac gctcggtgat ccgggcattg agcagtcatt
    52021 gaaaattatc gatacgctaa ttgaagccgg tgctgacgcg ctggagttag gtatcccctt
    52081 ctccgaccca ctggcggatg gcccgaccat tcaaaacgcc actctgcgcg cctttgcggc
    52141 aggtgtgact ccggcacaat gttttgaaat gctggcactg attcgccaga aacacccgac
    52201 cattcccatt ggcctgttga tgtatgccaa tctggtgttt aacaaaggca ttgatgagtt
    52261 ttatgcccag tgcgagaaag tcggcgtcga ttcggtgctg gttgccgatg tgccagttga
    52321 agagtccgcg cccttccgcc aggccgcgtt gcgccataat gtcgcaccta tcttcatctg
    52381 cccgccaaat gccgatgacg acctgctgcg ccagatagcc tcttacggtc gtggttacac
    52441 atatttgctg tcacgagcgg gcgtgaccgg cgcagaaaac cgcgccgcgt tacccctcaa
    52501 tcatctggtt gcgaagctga aagagtacaa cgctgcacct ccattgcagg ggtttggtat
    52561 ttccgccccg gatcaggtaa aagcagcgat tgatgcagga gctgcgggcg cgatttctgg
    52621 ttcggccatt gttaaaatca tcgagcaaca tattaatgag ccagagaaaa tgctggcggc
    52681 actgaaagtt tttgtacaac cgatgaaagc ggcgacgcgc agttaatccc acagccgcca
    52741 gttccgctgg cggcatttta actttcttta atgaagccgg aaaaatccta aattcattta
    52801 atatttatct ttttaccgtt tcgcttaccc cggtcgaacg tcaacttacg tcatttttcc
    52861 gcccaacagt aatataatca aacaaattaa tcccgcaaca taacaccagt aaaatcaata
    52921 attttctcta agtcacatat tcctcaggta attgttaata tatccagaat gttcctcaaa
    52981 atatatttcc ctctatcttc tcgttgcgct taatttgact aattctcatt agtgactaat
    53041 tttaatgagt gtcgacacac aacactcata ttaatgaaac aatgcaacgc aacgggagaa
    53101 ataacatggc cgaacatcgt ggtagttcag gaaatttcgc cgaagaccgt gagaaggcat
    53161 ccgacgcagg ccgtaaaggc ggtcagcata gcggcggtaa ttttaaaaat gatccgcaac
    53221 gcgcatctga agcgggtaaa aaaggcggtt aacaaagcgg tggtaataaa tcaggcaaat
    53281 cctgattcac cgttaattat taatatcagc tgataaagat aattgcatct gcgggccgta
    53341 tggctcgcag tgatttcact ggagaaaaat atgaatatga agaccattga agatgtattt
    53401 attcaactgc tttcagatac ctacagcgca gaaaaacaat taacccgggc actggcaaaa
    53461 ctcgcaagag caacatcaaa tgaaaaatta agtcaggctt ttcatgcgca cctcgaggaa
    53521 actcatggac agattgaacg aattgatcaa gttgtggaat cagaatcaaa tctgaaaatt
    53581 aagcgcatga aatgtgtggc aatggaaggt cttattgaag aagctaatga ggtcatcgaa
    53641 agtaccgaga aaaacgaagt gcgtgatgcc gcactgattg ccgcagcaca gaaagtcgag
    53701 cattatgaga ttgccagtta cgggacatta gcgacgctgg ctgaacaatt aggttaccgt
    53761 aaagcagcga agcttctgaa agaaaccctg gaagaagaaa aggccaccga catcaaactg
    53821 actgatctgg cccttaataa cgtaaataag aaagccgaaa ataaagcctg aaatatgaat
    53881 tttaactttt agtcatttta taaagaggac attttcatga atcgtattga acattatcat
    53941 gactggttac gtgacgccca cgcaatggaa aagcaagccg aatctatgct tgaatccatg
    54001 gccagccgta tagataatta tcctgaacta cgcgctcgta ttgaacaaca tcttagtgaa
    54061 accaaaaacc agattgttca actggaaact attcttgatc gtaatgacat ttcacgttca
    54121 gtcattaaag attccatgag taaaatggct gcgcttgggc agtcaatcgg tggtatattc
    54181 ccttctgatg aaatagttaa aggctctatt ggcggatatg tcttcgagca atttgaaatc
    54241 acctgttaca cctcactatt agcagcagca aaaaatgccg gtgatacagt ctcaattccg
    54301 atcatcgaag cgattttaaa tgaggaaaag caaatggccg actggctgat tcagcatatt
    54361 ccgcaaacaa ctgagaaatt tttaattcgt tctgaaactg atggcgtaga agcgaagaaa
    54421 taataccctt tataccatgt ccttattgac cccgtatatt acggggtcgt ttttgtgcgg
    54481 aattaaaaac gatatcctgc tgagaacata aacacccacg gatcgaggcg tacgctatcg
    54541 tgttgctgta caccgcctag cttataattg gcggtggtat cgatatccat gtaccacact
    54601 gacatgttaa ccaaccagtc acggttaatc agataatcaa cccccacctg cccggcagct
    54661 ccccaggaat ctttcagact gagatcggaa agccccgcct ctttgccatg atcgttaaat
    54721 ccattatcaa agaaggtggt gtagttaata ccggccccaa cgtaaggacg gaatttgctg
    54781 ctggcatcac caaaatacca ctgcgccatc agtgttggcg gcagatggtg aacggttgca
    54841 atatcgccgg tcgcccgggt gccgatttta tggcggaacg gcgtcgctgc cagtaattcc
    54901 acaccaatgt tgtcggtcgc catataagta aacgtcaagc ccagttgagt gttattggtc
    54961 acgctgaatc cacccagact tcctaacgta ccaccagcac cttctgttgg acgtacggtt
    55021 gcagaacctg cacgcataaa aaattcgcct gcttcatgcg caaaggcact gccagaaaga
    55081 agagttgtta ctgccaaagc cgccactgtt aactttttca tatccgctcc gtcgttatgg
    55141 ttataaaaac gtgacgaata tacctacatt ggagtaacaa atgatcctac atagatcaca
    55201 ttacatatga taattcacca tttattgatc tggattaatt tagaagttgc attgagaatc
    55261 ctggttaatg tgatttaaat caatttttaa atattttctc tacgcaaata ttaactcgat
    55321 cgccacttgc cgcgctttgc ccacgcctga aggccagcaa ttacctccgc tttacaaaga
    55381 tatgcttttc catacaagac ctgatgctgc cacccgaggc ttaaccaggg tacaattgcc
    55441 cgctaattaa cacctgcata aaactcaagg agagtgcatg tctatcacgg cgcagtccgt
    55501 ataccgtgac accggaaatt tcttccgtaa tcaatttatg accattctgt tggtatcgtt
    55561 gctatgtgcg tttatcacag tggtgttagg gcatgttttc tcacccagtg atgcacagct
    55621 tgcgcagctc aatgacggcg tgcccgttag cggcagtagt gggttgttcg acctggttca
    55681 gaatatgtca ccggaacagc aacaaatttt gctgcaggct tcagcggcgt ccactttttc
    55741 agaattaatc ggtaacgcca ttctcgccgg aggcgtaata ttaattatcc agctggtgtc
    55801 tgcgggtcag agagtcagtg cgctgcgagc tattggtgcc agtgcgccga tattgcccaa
    55861 gttatttatt ctgatttttc tgactaccct tttagtacag attggcatca tgctggtggt
    55921 cgttccggga attatcatgg ccattttact ggccctggca ccggtgatgc tggtacagga
    55981 caaaatgggc atttttgcct cgatgcgtag cagtatgcgg ctgacttggg cgaatatgcg
    56041 tctggtggca cccgcagtac tgagctggtt gctggcaaaa acactgttgc tgctttttgc
    56101 ctcttctttt gccgcattaa ccccggaaat tggtgccgta ctggcgaaca ccttgagcaa
    56161 cctgatttct gccatattgc tcatctatct gttccgcctg tatatgttga ttcgccaata
    56221 accttaagca tttgattacg gaatcgtaaa atgaagcagt ttcttgattt tttaccgctg
    56281 gttgtctttt tcgcgtttta caagatttat gacatctatg cggctactgc agcgctgatc
    56341 gtcgccacgg cgattgtgct tatctatagc tgggttcgct ttcgtaaggt tgagaagatg
    56401 gccctgatca cttttgttct ggtggtcgtc ttcggtggct tgacgctgtt cttccacaat
    56461 gatgagttta ttaaatggaa ggttacagtc atttatgctc tgtttgcggg agccctgtta
    56521 gtcagccaat gggtgatgaa aaagccgcta attcagcgga tgctgagtaa agaactcacg
    56581 ctgccgcaac cggtatggtc gaagctaaat ctggcctggg ctgttttctt tatcctttgc
    56641 ggtctggcaa acatctacat cgcattctgg ctgccgcaaa atatttgggt caactttaaa
    56701 gtctttggcc tgaccgccct taccttaatc ttcacattgt taagcggtat ctatatctac
    56761 cgccacatgc cgcaggaaga taaatcctaa ccagactgcc agtccttcgg ggctggcgct
    56821 gtatctcctc tgttctcctc aggaaaatca tagtagcatc gcgcctgtga ttttcctttt
    56881 aagtcggttt taccatgtct acaacacata acgtccctca gggcgatctt gttttacgta
    56941 ctttagccat gcccgccgat accaatgcca atggtgacat ctttggtggt tggttaatgt
    57001 cacaaatgga tattggcggc gctattctgg caaaagaaat tgcccacggt cgcgtagtga
    57061 ctgtgcgggt tgaaggaatg actttcttac ggccggttgc ggtcggcgat gtggtgtgct
    57121 gctatgcacg ctgtgtccag aaagggacga catcggtcag cattaatatt gaagtgtggg
    57181 tgaaaaaagt agcgtctgaa ccaatcgggc aacgctataa agcgacagaa gcattattta
    57241 agtatgtagc ggttgatcct gaaggaaaac ctcgcgcctt acctgttgag taatagtcaa
    57301 aagcctccgg tcggaggctt ttgactttct gcttactgaa tttcggtggt gccgttaatt
    57361 ttaaacagga tattcaccac aatcccactg cctggcttac ccggctcata acgccatctg
    57421 cgcatcgcat ttttcacctc acgctcaaac atgttcgcag gcttggctga gaggatttgt
    57481 acgttatcca cgcgaccatc cggcgtgacg tcaaatttaa ctttaacctg cccttcaatg
    57541 cgcaatgcct gtgctcgtgc cggatactgc ggctgattac ggcttaatgc gcgtggtcct
    57601 gaagccacac tggtaaccgg cttgctggtt gcagccgttg ccgtacttga tgtggggcgc
    57661 gcgggtgccg tattttcaaa cggtgatgcc ggacgcgact ctacgggttt gacatcgcgt
    57721 tttggctgct cctgtacctt tttcaccggc ttcggttttg gcttaggttt cggcttcggc
    57781 ttttcaatga ccaccggtgc ttcttttggc ggttcgggga tcggctcagg ttccggttct
    57841 ggctctacca ccggctccgg tggcggctga acggcttgtg gcggttcgag atcagcaggc
    57901 gcaaccatcg tgacagaaat tggttgcgca ggcgcaggta gttcaataac ctgatgtacc
    57961 gaggtataga gcagccccgc cacaacagca ccatgaatgc agaccgaaag taacgtcggc
    58021 caggggaagc ggcgaggtaa atcaagggtt attgaagtca taatcatttc agtagaaaaa
    58081 ctaggcctcg attttaaatg caaatagcaa tcatattcaa taaggcaaga cgatcaggat
    58141 cagtgaaaat gtcatttttg gcgtaattac ccttacttta tgcatgacct taacaaagac
    58201 atcatctttc cgttgcagat cttcgccctt tcaaataacg tactttacaa ctttcccgaa
    58261 caaggagttg tgcccgtgtt gtatgttatt tacgctcaag acaaagctga tagcctcgaa
    58321 aaacgccttt ccgttcgtcc ggcacattta gcacgtttac agttactgca tgatgaaggt
    58381 cgtttgttga cagctggtcc aatgccagcg gtagacagta acgatccagg ggctgcggga
    58441 tttactggct caaccgttat tgctgaattt gaatcgctgg aagcagctca ggcctgggcc
    58501 gatgctgacc cgtacgtagc ggcaggagtc tatgaacacg tttcagtgaa accatttaag
    58561 aaagtgttct gag
//
LOCUS       DAJTSA010000015        58032 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000015 DAJTSA010000000
VERSION     DAJTSA010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.015"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(24..278)
                     /locus_tag="OO369_000974"
     CDS             complement(24..278)
                     /locus_tag="OO369_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR5981339.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="OO369_000975"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="OO369_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR5981340.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="OO369_000976"
     CDS             1228..2694
                     /locus_tag="OO369_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5981341.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="OO369_000977"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="OO369_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR5981342.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="OO369_000978"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="OO369_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR5981343.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="OO369_000979"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="OO369_000979"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR5981344.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="OO369_000980"
     CDS             5709..7265
                     /locus_tag="OO369_000980"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR5981345.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDTPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="OO369_000981"
     CDS             7347..9161
                     /locus_tag="OO369_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5981346.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="OO369_000982"
     CDS             9162..10256
                     /locus_tag="OO369_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR5981347.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="OO369_000983"
     CDS             10256..11281
                     /locus_tag="OO369_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5981348.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="OO369_000984"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="OO369_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR5981349.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="OO369_000985"
     CDS             complement(12876..13220)
                     /locus_tag="OO369_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981350.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="OO369_000986"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="OO369_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR5981351.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="OO369_000987"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="OO369_000987"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR5981352.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="OO369_000988"
     CDS             15616..17376
                     /locus_tag="OO369_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5981353.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="OO369_000989"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="OO369_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR5981354.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="OO369_000990"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="OO369_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR5981355.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="OO369_000991"
     CDS             19113..19340
                     /locus_tag="OO369_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR5981356.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="OO369_000992"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="OO369_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR5981357.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="OO369_000993"
     tRNA            21195..21271
                     /locus_tag="OO369_000993"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="OO369_000994"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="OO369_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR5981358.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="OO369_000995"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="OO369_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR5981359.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="OO369_000996"
     CDS             complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="OO369_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR5981360.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="OO369_000997"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="OO369_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR5981361.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="OO369_000998"
     CDS             complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="OO369_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR5981362.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="OO369_000999"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="OO369_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR5981363.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="OO369_001000"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="OO369_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR5981364.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="OO369_001001"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="OO369_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR5981365.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="OO369_001002"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="OO369_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR5981366.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="OO369_001003"
     CDS             complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="OO369_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR5981367.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="OO369_001004"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="OO369_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR5981368.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="OO369_001005"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="OO369_001005"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR5981369.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="OO369_001006"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="OO369_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR5981370.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="OO369_001007"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="OO369_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR5981371.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..36538)
                     /gene="napA"
                     /locus_tag="OO369_001008"
     CDS             complement(34052..36538)
                     /gene="napA"
                     /locus_tag="OO369_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR5981372.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36535..36798)
                     /gene="napD"
                     /locus_tag="OO369_001009"
     CDS             complement(36535..36798)
                     /gene="napD"
                     /locus_tag="OO369_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR5981373.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36788..37282)
                     /gene="napF"
                     /locus_tag="OO369_001010"
     CDS             complement(36788..37282)
                     /gene="napF"
                     /locus_tag="OO369_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR5981374.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37391..37555
                     /locus_tag="OO369_001011"
     CDS             37391..37555
                     /locus_tag="OO369_001011"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981375.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37690..38178
                     /gene="eco"
                     /locus_tag="OO369_001012"
     CDS             37690..38178
                     /gene="eco"
                     /locus_tag="OO369_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR5981376.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38300..39331)
                     /locus_tag="OO369_001013"
     CDS             complement(38300..39331)
                     /locus_tag="OO369_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCR5981377.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39565..39924)
                     /locus_tag="OO369_001014"
                     /pseudo
     CDS             complement(39565..39924)
                     /locus_tag="OO369_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40142..41785)
                     /gene="yojI"
                     /locus_tag="OO369_001015"
     CDS             complement(40142..41785)
                     /gene="yojI"
                     /locus_tag="OO369_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR5981378.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41861..42511)
                     /gene="alkB"
                     /locus_tag="OO369_001016"
     CDS             complement(41861..42511)
                     /gene="alkB"
                     /locus_tag="OO369_001016"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR5981379.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42511..43575)
                     /gene="ada"
                     /locus_tag="OO369_001017"
     CDS             complement(42511..43575)
                     /gene="ada"
                     /locus_tag="OO369_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR5981380.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43649..44704)
                     /gene="apbE"
                     /locus_tag="OO369_001018"
     CDS             complement(43649..44704)
                     /gene="apbE"
                     /locus_tag="OO369_001018"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR5981381.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44816..45937)
                     /gene="ompC"
                     /locus_tag="OO369_001019"
     CDS             complement(44816..45937)
                     /gene="ompC"
                     /locus_tag="OO369_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5981382.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46675..49347
                     /gene="rcsD"
                     /locus_tag="OO369_001020"
     CDS             46675..49347
                     /gene="rcsD"
                     /locus_tag="OO369_001020"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR5981383.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49364..50014
                     /gene="rcsB"
                     /locus_tag="OO369_001021"
     CDS             49364..50014
                     /gene="rcsB"
                     /locus_tag="OO369_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR5981384.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50214..53063)
                     /gene="rcsC"
                     /locus_tag="OO369_001022"
     CDS             complement(50214..53063)
                     /gene="rcsC"
                     /locus_tag="OO369_001022"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR5981385.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53338..54114)
                     /locus_tag="OO369_001023"
     CDS             complement(53338..54114)
                     /locus_tag="OO369_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR5981386.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54119..55768)
                     /locus_tag="OO369_001024"
     CDS             complement(54119..55768)
                     /locus_tag="OO369_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR5981387.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55769..56328)
                     /locus_tag="OO369_001025"
                     /pseudo
     CDS             complement(55769..56328)
                     /locus_tag="OO369_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56487..>58032)
                     /locus_tag="OO369_001026"
     CDS             complement(56487..>58032)
                     /locus_tag="OO369_001026"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5981388.1"
                     /translation="LTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRN
                     NVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDK
                     QGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDK
                     PAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQ
                     NGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFR
                     NNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAG
                     PGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPL
                     LHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESP
                     LADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQT
                     AAILMV"
BASE COUNT        14195 a        15654 c        14261 g        13922 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttatg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac actccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccgtgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc gacagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gcggcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgact atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgcgg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgacaacccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctaccg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctaaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcaaatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccagttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgccag cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct agtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagacg ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atggcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ttttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggcc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagggta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga accagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata ataaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgaaggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcagcaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgagct ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 ggatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatctct tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gttaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaagtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cttctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcta gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc aggatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgcgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacagacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggg
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtaactctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc agggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtggc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag ggagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgtta gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aatgatatag ttggcgatgt agttcaggat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac tgttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcagggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcgct ttatcccatt
    36421 taatggcttc ctgctgacca acaacggcgc gggcaacgcc cggcacgctg agaccggcag
    36481 ccgccgcagc ggccgcaacg gcgttagctt tcataaagct acgacgactg agtttcatgg
    36541 tgtttcctca ccttgctctt cctgctggtg ataaaccagc gacaccgcca gcacgccctc
    36601 tacgttgcgt actgactcaa tggtttggat cagcgtttcg ctgtcttctg cttccaccac
    36661 cacaatcaac tgaccgctcg gcgcgtcgct gacagcaact tcacagccgg gaaaggcgtt
    36721 cagttgggtg ctgatgtctg aaattcgttc gcttttggcc tgcacgacca ggctgcaaac
    36781 ttgccagtta gtgtgcatgg agatactccg cagttatggc tgataccgga cagctggctg
    36841 cacatgctcc acatccgttg caaagttggc tattaagttg cgcctggtag atcccggaga
    36901 gcgtcgggcg aaagataatt gccattggct cacagctatc ctgacagcgg cggcattcaa
    36961 ctgactgata cgccaggcac gcgtccccga tggtgaactg caaatcccag gccctggtgt
    37021 ggcgcggaga aaatagcgat tcggggcaag cctgcgcgca ggcgtaacag aagctgcact
    37081 cgttattttt gaaattaacg ctcggatagc cacccgtgcc gcgttgcaga atgttgtttt
    37141 cacaagcatt aatacaggcg tcgcagcggg tgcaatgggt cagaaaatga gattcatcac
    37201 cggaccaggg cggacggata ccgttactgg ctttgcgcca gcaaccagtg agtatgcccc
    37261 gacgggatgc atcaatcttc acattgacct tccatcatta acgcgctctg aaaattgaga
    37321 gcgaccaaat agaccgcata attaataagc catttttata gccgctaaga tattaaagga
    37381 tgtgtcaaag atgcataccc cgatcggggt aaaacctgta gcaggatcaa aagagtggcg
    37441 ggaagcgtgg caaaaacggg cttttgctca catttcaaat ggttataaac atatttatat
    37501 agcgattaat tcaccagaga tatttctgct ggtttgctcc ctcatcagaa tttaacacta
    37561 aaagagcagg taaaattgtc tgaatgttct ttaagttatt cataaagcaa attaataaat
    37621 ctgatgaata tgttaacctt cagcgacatc atcggtgaaa acctataaat gaagaaggaa
    37681 agcaaaaaaa tgaagaccat tctacctgaa gtattgtttg ccgctttcgc taccacttcc
    37741 gcctgggcgg cagaaagcgt ccagccactg gaaaaaatcg cgccttatcc acaagctgaa
    37801 acaggcatga agcgtcaggt gattcagtta accccgcaag aagatgaatc taccctgaaa
    37861 gtagaactgt taatcggtca gacgctggaa gtcgattgca atttgcatcg tctcggcggg
    37921 aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt attatgtctt tgataaagtt
    37981 agctcgccgg tttccacgat gatggcctgc ccggatggca agaaagagaa gaaatttgtc
    38041 accgcgtatc tgggcgatgc tggaatgctg cgttacaaca gcaagctgcc gatcgtggtg
    38101 tatacgccag acaatgtaga tgtgaaatac cgcgtctgga aggcggaaga gaaaattgac
    38161 aacgcggaag ttcgctaaac tgttgtgaag tacggcaccc cgttggttgg ataaggcggt
    38221 tacgccgcat ccaacatcta acgcccgagc cggttgcctg atgcgacgct ggcgcgtctt
    38281 atcagtatac gccacatcat tacaacgcaa tatccgccac ttctttttga acgggttgcg
    38341 gtttcaactg cggtttcggc gtactatctg ctgcttgcgg tttgtcgtat ttcagcccca
    38401 gcacttcgct ggtgtactgc aactcgcgtt ctgttgccgc tacatcaccg ttcagcttgc
    38461 gtccataaga cggaacgatc gctttcaacg tagcctgcca ttgcgggctg gaaacgcgat
    38521 cgccaaatac tttttccagt agattcaaca taatcggcgc ggcggttgac gcccctggcg
    38581 atgcccccag cagcgccgca atggtccctt gctggtcact gaccacttcg gtacccagac
    38641 gcagtacgcc acctttctcg gcatcacgct tgataatctg cacacgctgc cccgcttgcc
    38701 acaaacgcca gtcctctttt ttcgcttgcg gatagtactc tttcaacgct tcaaaacgat
    38761 cctcttcact caacatcacc tgactcacca gatatttcac cagatcgaaa ttatccagcc
    38821 cgacgtgcat catcggcatc acgttagagg tggtggtgga actcatcaga tcccacaatg
    38881 aaccgttttt gaggaatttg gttgagaagg tggcaaatgg cccaaacagc actacgcgtt
    38941 taccgtccag aacacgggta tcgatatgcg gaaccgacat cggtggtgcg ccaacggatg
    39001 ctttaccgta aactttcgcc agatggcgat taaccacgtc tggattttcc gaaacaagga
    39061 actgtccgcc caccgggaaa ccggcgtagt ctttcgcttc cggaatcccc gattcctgta
    39121 acagcttcag cgccgcaccg cccgcgccga taaagacaaa tttggcgcgg atgttctgtg
    39181 cagtgccgtt tttcagatcg gcaacggtaa cggtccaggt gttatcgtca ttacgcttta
    39241 gggcgcggac ttcgctgctg agttgcagcg agaagttaga tttcttctgt aaggaagcaa
    39301 ttaactggcg ggtgatctca ccatagttca catcagtgcc aatttctgtt gcggatcgcg
    39361 cccttccatc actaacggtg cccactcttt gatctgcgcg tgatcttcag agtaacgcat
    39421 accgcgaaac agcgagcttt gttgcaacgc ggcgtaacgg gcgcgcagga aattaacgtt
    39481 atcctcgccc cagacaaagc tcatatgcgg aacggtattg ataaatgaac gcggagtacg
    39541 cagcacgccg cgctcgacct ggtgcgccca gaactggcgg gaaatctgaa atgcttcgtt
    39601 aatggcgact gctttttcaa tactgatgct gccatcggcg ttttgcgggg tgtagttcag
    39661 ttccatcagt gcagaatgcc cggttccggc gttgttctag ccgttcgaac tctcctgcgc
    39721 gcccccctcc aggcgctcca ccatggtcat cgaccattca ggctccagct cgcgtcaata
    39781 ggtccccaac gtggcgctca taatgccgcc gccaatcaac agtacatcag tttcctgctc
    39841 ctcggacgct ttcgcttttg ccgccatcga aacggcatta agccccacgg ccatcgagaa
    39901 gagcatggca gtcacttttt tcatcttgtt aatgccttac ttttagtcgc tttattgcag
    39961 gtgagatttg cgcggcatca acggtaacac ttaagtaaca acatttaaat aatgtttata
    40021 aattatattc taattttaga aacattataa tttcgttgat taattatagg gttattagaa
    40081 atgaagggat ttttagcata ttgacctgac ggcagcagtc cgccaggtca gcgtcgtaat
    40141 attatgccgt ccgggcaacg gcatcacgcg aagcggcatc gcgctcttcg cccgtcagct
    40201 cgctaagttg cccattgcgc atttccagca ggcggtcggc gtggataaag taatgatcat
    40261 catgactgat agcgaaaata gttttaccca tctcctgcat cagcggcagc aacacctgat
    40321 aaaactcacg acggaagtgt ggatcctgat ccaccgccca ttcatccagc aggataatat
    40381 cgcgttcttc tgccagcgcc agcaacagcg ccacgcgttt tttctgccct tttgataact
    40441 tcaggttaac aatacgcccg ttgcttaact caagcttatg agccattttc agctgcgcca
    40501 gccacttctc aaccagttgc gggttagcgg gttgcccctc cggccccagc agttgatcaa
    40561 acagccagac atcggtaaac actgccgaaa acagtttgcg ataatcttcc ggttgttcgc
    40621 cgctgacagg tttgccatcc agcaagattt cgccgctttg tggctgatac aagcccgtca
    40681 acaacatcgc cagcgtcgat tttccgctac cgttaccgcc aatcagaaac agcagctcgc
    40741 cacgtttgat ggtgagatta atcggaccaa cggaaaacgc gttatcctga taagcaaacg
    40801 tcacgttacg cagctccagc gtttgccagt tgggaaacgc ctgcgggcgc ggaaactcag
    40861 ctttgaaagg cgcgagcgcg aatttgttca gcttgttaaa cgccacctgc gccgtcagca
    40921 gcgtcggcaa tgcgccaacc gccgaaagca gcggcgtacg caggaataac agcgtcaacg
    40981 aataggtcgc agcaacgttg gtatcagccc aaccgagact gttcgccatc cagaacacca
    41041 ggccgatagc gcccagcatc atgatgtttg accagttcac ggcactaaga tggaaggtgt
    41101 ctgcgcgaat aatatgatgg cgatactctt gcgcatcggg gatgtagagg ttgttaaaca
    41161 catactcggc gcgttcccgg ttcagagtca gctctttgcg cccttcaagt acagtttgaa
    41221 aatccgtgta cagcttgtct tcggtttcac gtagggtcgc catatgtttg tacacccgcg
    41281 ccaccagcac aaaaccgccc cagatggtga tcgccatcca gatagccgtt accagcaaca
    41341 ttttgcccga cagcatccac agatacgccg atgaaccgat agtgagaatg atcccctgcc
    41401 ccagttccgg cagacgcaca aaagcaatgg tgatattgcg cacatcgctg gttaatcccg
    41461 ccagcaacga ggcgctaccg agttgttcaa tgcgctcgac gtgggtatcc agaatccgct
    41521 tgataaattc actacgcagt cggtaaacga agtgatgccc caaagtggtg agcgccagtt
    41581 gcgatccgag agtgactgcc atcaacagca gcaataatcc cagaaactcc ggtaacacca
    41641 gcagactggt atccgccgtt tcgataaggc gctgattgat aaaagcaatt aagccaatgc
    41701 ctaatgccgc actggcgagg cttagcgcca tcacactgat aaatggccag cgatactgcc
    41761 gccagacaag tacaagaagt tccatgcaga aaacccggac aatgaattac agcccgcagt
    41821 ttaaacatct tcgcgcgcac agcaataata attcttattt ttattctttt ttacctgcct
    41881 gacggaatgt caggttgtag cggcagtcgg tggtgagtgg atgaaacccc gctttcaacg
    41941 gttgaatacc gtgataaaac agccgcgatt caccgcccca taccaccaca tcgccatgtt
    42001 ccaacaacaa acgtttgagc ggatcattac gtttcaggcc accaaattga aaaatcgcgg
    42061 gtaagcccag agaaacagaa acaattggcg cgcgcagatc cggttcgtct ttatcctgat
    42121 gcagcgacag tttcgcgcca ggagcgtagc ggttgataag acaagcatct ggctggaaat
    42181 ctggatagcc cgccgccgta gccgcacgtt gacataaatc atgaaagctt tgtggcatag
    42241 cgggccacgg tttatttgtt tgcggatcaa tgggcgaata gagataacct tgccgatggg
    42301 tcgtccagcc cagatgccca cagttggtca tcgccaccga catggtatat ccgcctggag
    42361 cgaccatctg gcgaaacggc gactggctgg caacgtcatt aatatcgcgg attagttgct
    42421 ccgcagcatt aaaagcaaaa cgccgtaaaa ttaccgcacc agccgccagt ggctcttgcc
    42481 acggttcagc atcggcaaac agatccaaca ttacctctcc tcattttcag cttcgcggcg
    42541 cagcagttgc gctttacgcg acacgcccca gcggtaaccg gaaagtgtgc catcaccacg
    42601 gaccacccga tgacagggta ttacgatagc cagcttgttg gcggcacagg cgctggcaac
    42661 ggcccgtacc gctttcggtt tgccgatggc gttagccagt tgctgataac tgacggtttc
    42721 accacaaggt atcgtgcgca gtgcctgcca gacttgttgc tgaaaagcag tgccgcgaat
    42781 atccagcggt aacgtcagcg gcgtatcgcg ttgattgagg ctggcgatca cttcacgcac
    42841 atgttgctga aacgtcagat cggcaggcgc gttgtcggca gcgggaaaca tctgctgcaa
    42901 ctcgctgatt aatgtcgcgt catcatcgcc cagcaatatc gcgcaaatcc cccgctcgct
    42961 ttctgccacc aggcaacgac ccagctcaca atcagccagc gcgtaacgca ccgccagatt
    43021 ttcgccaccg tgacggaatt gtttagccgt catgcccagc gtttcatcgg ctttgcggta
    43081 gtaactgctg ctgtcgggga atccggcgtt aagaatagac gtcgtcacgc tctccccttt
    43141 cgccagcgat tcgcgcaaac ggcgagcgcg ccaggcctgt tgccaggctt taggcgtcat
    43201 tccggtagta gctttaaaca accgatgtag atgaaatgga ctcatcgcca cctggtcggc
    43261 taaggcttcc agcgttacag gcgtttcctg ttccagcagt cgacacgcgt gggtgatttt
    43321 atccaaccga tgttgccggg gattggcttt gtctggctga caacgtttgc aggggcgaaa
    43381 tccggcggcg agtgcctcgc tggcatttgc gtagaaggag acattttccc gcaaggcatg
    43441 tctggcgcgg caagaaggac ggcaaaagat acccgtggtg cgcacggcga aaacgaattc
    43501 gccgtcggca ttcgggtcgc gggctaagac agattgccag cgttgatcgt cagttaagca
    43561 tgtggcgttt ttcataatca gctccctggt taaggatagc ctttagtctg cccggtcacc
    43621 atcacgcaaa aaccaacaat cttgcgcttt aatttttttc gctgacaagg aagcttttaa
    43681 actgtggtga catccaggtt ttaaagctat cgccttcttt ggtgatcata taaaccgcca
    43741 gcccttcccg gcgaacaacc tctttggctt tctccggccc gagtaccatc aagccagtat
    43801 cccaggcatc ggcttccagc gccgtcggag caatcaccgt cacggatacc agattgtgtt
    43861 cgatgggacg cccggtttgc ggatcgataa catgggaaag acgtttgccg tccagttcgt
    43921 aatagttacg gtagctgcca gaggtgctaa tcccatgacc gttgatatcc accacagcct
    43981 gaaccgcgtt ttctttatcg gttggttttt gaatcgctac ccgccacggc tggccttcac
    44041 cgttcatacc acggctgttc agcgcgccgc ccaccgacac cagatagcgg gaaatccctt
    44101 cctgctccat caagcgtgcc aggtgatccg ccgcataacc ttcaccgacg gtggagagat
    44161 cgacatataa atccggcagg tctttttgca gatattgctg atgcgactga ttgatgaccg
    44221 tcaggtgctg taagccggtt ttggctttca tcgcatcgat ctgttcctgg ctcggaattt
    44281 gaaccggctg ttgttccggg ccaaagcccc acagattcac cagcggcccg acggtaatat
    44341 ccatcgcacc atcggtcctt gccccaatgc gcagcgaggt ggtgacgata tcggccatcg
    44401 cttcacttac cggccacggc gacaaacttt gcgagtcgtt aaagcgcatc agcgcggaat
    44461 cttttttata ggtcgaaagc agctgatcgt cggcgtccag ctgggtctga atcttttctt
    44521 taagttcggc actgcgtttg gcatcaatgc ccgggatgct ggcacgccag aaggtaccca
    44581 tggttttgcc ttcaagaacg gtaacttcag tagcgtgggt tttggcgggt tgtggttttt
    44641 gatcgcaacc aacaaagaag agcgcggcaa ccagcagtgc cacgcgggta aagcttattt
    44701 ccatacgtga ttatcctcat gcgaacggtc gcaagagtac accaaaaaac tgtgtttgta
    44761 cgctgaaaac aatgaaaaaa gggcccgcag gccctttgtt cgatatccat cgagattaga
    44821 actggtaaac cagacccaga gctacgatgt tatcagtgtt gatgccagcg tcacgagtga
    44881 actggttgtc gtccagcagg ttgattttgt agtcaacgta ggtggacatg tttttgttga
    44941 agtagtaggt cgcaccaaca tcaacatatt tcaggatatc ttcgtcgtcg tagttacgac
    45001 cattgatgac acccaggttt ttacctttag actgcaggta agccagggac ggacgcagac
    45061 cgaagtcgaa ctggtactga gcaacagctt cgaagttctg tgctttgttc gcccaaccca
    45121 agttgcctac gcgagttgcg ttgtaggtct gggtgtactg agcagctagg tagatgttgt
    45181 tcgcgtcgta tttcagacca ccggtgtagg tttcagcgcg gtcgccatta ccgatgtaac
    45241 gctcatcata acggttcaga ccgaagttct ggtcatcagt acgtttggag ctggaaactg
    45301 cagcaccgat accgaagcct tcgtaatcat aagtaataga accgccaacg ccgtcaccgt
    45361 tctgacgcag agcttcacga ccgttgttgg tcatgccttc accttccggg ctaccgtttt
    45421 tgccctgata ctgaacagcg aagttcagac cgtcaaccag accaaagaag tcggtgctac
    45481 ggtaggttgc gaagccgtta ccacgctgct gcatgaagtt gtcagaaccg taagtgtcac
    45541 caccgaattc tggcagtacg tcggtccagg aagttacgtc gtaaactacg ccgtagttac
    45601 gaccgtagtc gaaagaacct acatcctgga atttcagacc tgcgaatgcc acacgggtcc
    45661 aggagttgtt ttcgttttca gcgctgttgc cctggatctg atattcccac tggccgtaac
    45721 cggtcagctg gtcagtaacc tgagtttcac ctttgaagcc aagacgcatg taggtctggt
    45781 cgccatctac agacttgtcg tcagagaaat agtgcaggcc gtctacttta ccgtacagat
    45841 ctaatttgtt gccgtctttg ttgtaaactt cagcagcgtt tgctgcgcct gctaccagca
    45901 gagctgggac caggagggac agtactttaa ctttcatgtt attaaccctc tgttatatgc
    45961 ctttatttgc ttttttatgc cactgcatac tgattaaccc tcattaatca gtcggcaagt
    46021 ccattctccc caaaaatgca gaataatcca acacgaatat gatactaaaa cttttaagat
    46081 gtttcattta tcgctataga tgtttcaaaa tgtaaataca agggaacttt ttaagattat
    46141 tgcggaatgg cgaaataagc acctaacatc aagcaataat aattcaaggt taaaatcaat
    46201 aacttattct taagtatttg acagcactga atgtcaaaac aaaaccttca ctcgcaacta
    46261 gaataactcc cgctatcatc attaacttta tttattaccg tcattcattt ctgaatgtct
    46321 gtttacccct atttcaaccg gatgcctcgc attcggtttt tttacccttc tttacacact
    46381 tttcattatt ctgtgctacc acagaaaaac tataacgctt gttaactatt tcacaaataa
    46441 ttaacatcag cataatttcc agcaatcttt gtttatttgc aattaatttc gttgggcttt
    46501 ttgtaggtta tttgtacagc aaaatggcgc ttgtacatct atttcccccc atacaggatg
    46561 ataaatatca cgggagaata gagaatcatc aatcaggtaa gagtctggaa tttcacactg
    46621 taccctttat actgccctat cacttcgcga agttttaaca ggtcataaac acgaatgcgt
    46681 cagaaagaga caacggccac gacccgcttt tcactcctac cggggagcat tacccgcttc
    46741 tttttactgt tgatcattgt gttactggtg acgatggggg taatggtaca aagtgccgtt
    46801 aacgcttggc tgaaagataa aagttaccag attgtcgaca ttacccacgc aatccaaaag
    46861 cgcgtcgata cctggcgtta cgtgaccagg cagatctacg acaacattgc cgcgacgacc
    46921 tccccctcct ccggcgaagg tttacaagag acgcgcctga aacaggatgt ctactatctg
    46981 gagaaaccac gccgcaaaac ggaagcgtta atctttggct ctcatgacaa ctcaacgctt
    47041 gagatgactc agcggatgtc cacttatctg gatacattgt ggggtgcaga aaatgtaccg
    47101 tggtcgatgt attacctgaa tggtcaggat aacagtctgg tgctgatctc aaccctaccc
    47161 cttaaagatc tcacctccgg atttaaagaa tcgaccgtca gtgacattgt tgattcacgt
    47221 cgtgcagaaa tgttacaaca ggccaacgcc ctcgatgaac gcgaaagttt ttctaacatg
    47281 cgccgcctag cctggcagaa cggtcattac tttaccttgc gtaccacgtt caaccagccg
    47341 ggacatctgg caacggtcgt ggcttttgat ctgccgatta atgatttgat cccaccgggt
    47401 atgccgctgg acagtttccg ccttgagcca gacgcgacgg caacgggaaa caatgataat
    47461 gagaaagaag ggacggatag cgtcagtatc cactttaaca gtacgaagat tgaaatctcc
    47521 tcggcactca actctaccga tatgcgcctg gtctggcagg ttccttatgg caccttattg
    47581 ctggatacgt tgcaaaacat tctgctgcca ctgctgctga acatcggttt gctggcgctg
    47641 gcgttatttg gctataccac attccgccat ttctccagcc gcagtacaga aagcgtcccc
    47701 agcacggcgg tcaataacga attgcgcatt ttacgggcaa tcaatgaaga gatagtctca
    47761 ctgttgccgc tcggcctgct ggttcacgat caggaatcga accgcactgt cataagtaac
    47821 aaaattgccg atcatttgct gccgcatctg aatctgcaaa acatcaccac catggcggaa
    47881 cagcatcagg ggattattca ggcgacgatc aataacgagc tgtatgagat ccgcatgttc
    47941 cgcagccagg tcgcgccgcg cacacaaatt ttcattattc gcgatcagga tcgcgaagtg
    48001 ctggtaaaca agaaactcaa gcaggcgcag cgtctgtatg agaaaaacca gcaggggcgg
    48061 atgaccttta tgaaaaacat tggcgatgcg ctgaaagaac ccgcacagtc cctggcggag
    48121 agtgcggcta aactcaacgc cccggaaagc aaacaactgg cgaatcaggc agatgtgctg
    48181 gtgcggctgg tcgaagaaat acagttagcg aacatgcttg cggatgatag ctggaaaagt
    48241 gagacggtgc tgttctccgt gcaggattta attgatgaag ttgtgccttc agtgttgcct
    48301 gccatcaagc gtaaaggtct gcaactgctg attaacaatc atctgaaagc acacgatatg
    48361 cgccgcggcg atcgcgatgc cttacgacgt attttgctgc tactgatgca atatgccgtg
    48421 acctcaacgc aattgggaaa aatcaccctt gaggttgatc aggatgagtc ctctgaagac
    48481 cgcctgacat tccgcattct ggacacggga gaaggcgtaa gtattcatga aatggataat
    48541 ttgcacttcc cgtttatcaa ccagacccaa aacgatcgtt atggaaaggc ggacccgctg
    48601 gcattctggc tgagcgatca actggcacgt aaactgggcg gtcatttaaa catcaaaacg
    48661 cgggatgggc ttggtacacg ctactctgtg catatcaaaa tgctcgccgc tgacccggaa
    48721 gttgaagagg aagaagagcg tttactggat gatgtctgcg taatggtgga tgttacttcg
    48781 gcagaaattc ggaatattgt cactcgcctg ttagaaaatt ggggtgcaac ctgtatcaca
    48841 cccgatgaaa gattaattag tcaagattat gatatctttt taacggataa tccgtctaat
    48901 cttactgcct ctggcttgct tttaagcgat gatgagtctg gcgtacgaga aattgggcct
    48961 ggtcaattgt gcgtcaactt caatatgagc aacgctatgc aggaagcggt cttacaatta
    49021 attgaagtgc aactggcaca ggaagaggtg acagaatcgc ctctgggcgg agatgaaaat
    49081 gcgcaactcc atgccagcgg ctattatgcg ctctttgtag acacagtacc ggatgatgtt
    49141 aagaggctgt atactgaagc agcaaccagt gactttgctg cgttagccca aacggctcat
    49201 cgtcttaaag gcgtatttgc catgctaaat ctggtacccg gcaagcagtt atgtgaaacg
    49261 ctggaacatc tgattcgtga gaaggatgtt ccaggaatag aaaaatacat cagcgacatt
    49321 gacagttatg tcaagagctt gctgtagcaa ggtagcctat tacatgaaca atatgaacgt
    49381 aattattgcc gatgaccatc cgatagtctt gttcggtatt cgcaaatcac ttgagcaaat
    49441 tgagtgggtg aatgttgtcg gcgaatttga agactctaca gcactgatca acaacctgcc
    49501 gaaactggat gcgcatgtgt tgattaccga tctctccatg cctggcgata agtacggcga
    49561 tggcattacc ttaatcaagt acatcaagcg ccatttccca agcctgtcga tcattgttct
    49621 gactatgaac aacaacccgg cgattcttag tgcggtattg gatctggata tcgaagggat
    49681 cgtgctgaaa caaggtgcac cgaccgatct gccgaaagct ctcgccgcgc tgcagaaagg
    49741 gaagaaattt accccggaaa gcgtttctcg cctgttggaa aaaatcagtg ctggtggtta
    49801 cggtgacaag cgtctctcgc caaaagagag tgaagttctg cgcctgtttg cggaaggctt
    49861 cctagtgacc gagatcgcta aaaagctgaa ccgcagtatt aaaaccatca gtagccagaa
    49921 gaaatctgcg atgatgaagc tgggcgtcga gaacgatatc gccctgctga attatctctc
    49981 ttctgtgacc ttaagtccgg cagataaaga ctaatcatct gtaggccaga taagacgcgt
    50041 tagtgtctta tctggcattt gcaccgattg ccggatgcgg cgtaaacgcc ttatccggcc
    50101 tacgattccc attatttcaa cagattacat taaagtaggc cagataagac gcgtcagtgt
    50161 cttatctggc atttgcactg aatgccggat gcggcgtaaa cgccttatcc gtcctacgaa
    50221 tcccgcgatt tcctgaccct ctcggcatat accgtcagcg tctgttttat cacatccagc
    50281 gttaccggct tcgagaggca gctgtccata ccggactcca gacaccgctg cttctcttca
    50341 gccaacgcat tagcagttac tccgattacc ggcaacgtca gtcccaactg acgaatgcgt
    50401 tgcgtcaagc ggtaaccatc catatttggc atgttgacgt cgctaagcac gatatcaata
    50461 tgattcttac taagtacatt aagcgcatcg acgccatcat tcgcggtttt acattgatag
    50521 cccaacgatc ccaactgatc tgccagcaga cgtcggttaa tcggatgatc atccacgacc
    50581 agaatcatca tatcgtcgtt atcgctgacc gctttgtccg tcgacggcag agcgttagca
    50641 ggatcgtcgc tctccatctc gatcaaataa atacgcgcca acaatgccgg tagctcatgc
    50701 ggagcagcca cactgtgtac ccactcccct ggcgctttct ccagcggaat accaatatgg
    50761 cgacgacaga aggtcactac cgctctgccc tgccattttt tactcactac ctcgtcagtg
    50821 attaacacat cttcgggagt cggttcctgc ccttcgtatg ttgtaacgac gatgccgctg
    50881 cgctgcaaac tggtttccag gaactgacag agcgacgcat tgcggaccgc cagccagcag
    50941 cgtttaccac tcaacccttc cacgcctttt ttctgcgggt actgagcgcc gtacaacgga
    51001 atacgcacgg taaactggct gcccattccc ggttctgaat ctaccgagat atcgccgtcc
    51061 atcatgctga tcagtttttc acaaatcgcc agacccagac cggtcccctg gaagtttcgc
    51121 tgtacgcccg ttccaacctg gaagaaggga tcaaacaagc gcaccacttc tttcgccgga
    51181 atccccacgc cggtatcgcg aacacggata gagagataat cgccatcagc gcgaacatgc
    51241 aaaactatac agccggtatc ggtgaatttt atggcgttac tcaacaggtt ggagatgacc
    51301 tgctgtaaac gcatcgggtc gccatttaag gccactggca catccggttc aataaagcag
    51361 tacaagccta actgcttgcg taccaccagc ggtaaatagt tggcggtgat gtggttcatc
    51421 acttcacgcg gtgaaaactc acgcggttcg atcttcaact gttccgattc aatcttcgag
    51481 aaatcgagaa tatcgctgat aattttcaac aacaggctgg aagagttgtt cattgccgtc
    51541 accagacgat cgacgccttt cggtaactct ttggtttgca acagatccag gttaccgata
    51601 atgccataca gcggcgttcg cagctcatga ctgacggtgg caaggaacat cgattttgac
    51661 tggctcgcct gttccgctgc ttgtgccatc tcctgcaacg actcttccat cttcacgcgc
    51721 gaagaaacat ccaccagcac acaaatggcc acgttttcat tacgatagcg cgaatggacg
    51781 aagctgattt gcagattggt attgttgctg gtcaggacat caacaaaatt gacctgctgc
    51841 ccacagataa tttgcgtcag tcgttggcgg tcctcatgcg taagcatatt gagataggta
    51901 tgcgccagtt cgttacttaa aatattgacg ccatcagcgg tacgcaaaat gcagataccc
    51961 actggcgcgg aggcgacaat cttgcgattg aactgctcat gttcttccag tcgcagggcg
    52021 tcgctttccg ccggaatgaa aatacgtcgc tcgtacatcc gcgcgagagt aaacaatgca
    52081 gctccggcaa gcacattcag caaaattgcg ttaaggatca acatgcgaat gcgttccagc
    52141 accttatcaa ccggcaccga atacacgatg cttagcgatg agggtggcag atttttcttc
    52201 agcaccagct cccggaaccc ttccgtatag ccaaaccagg agcgttcctg catccagcga
    52261 ggatcgccct taattttact ttctggtccg gtaagcgaaa tcagggtatg accattttca
    52321 tcaagaatgg taacccccat cggcaacgta cccggtaaga aaaagttctc catccggatg
    52381 gtctgctcga cacccaaaag cgcctgcaac cggttcgcca gataaactgg cgtcaacgcg
    52441 taaaaatacc cgacgcaagg gcgcggacct tcgctgatcc agtagaggtt actgccgcta
    52501 tcatcttgtg gtgcatttcg atatttattg atgcgttcat gcaacgcttt caacgcggta
    52561 tcgcgttcca ctggcatatc acgcagaccg aaattggcca tgcagaggtt atcactgccg
    52621 attaaaaata cccggttgag atcgtaagcc gcagaaaaat tatcgcgcca gtagcgcata
    52681 aaccacgcca atgactccag agaacctcgc caggtgttac tcattgcgga acaatcggag
    52741 tcggcaaaca gcggttcaaa cgcaggcaca tccgcctgcg tttctcgtcc acgcggggaa
    52801 agcacaccgt tttctgccga taagcgattt tcggcgatgt acttaagctc tttcatcaca
    52861 tcagaggtgc gttgaataaa gcgctgagcc tgatcggaac tcagattaaa ttcctgacga
    52921 atttccgatt ctcgctgatg taacgcatta acgatgtaaa aaacggatga aaaagcaatc
    52981 aacagccaga gcactaacgc caatgctctg aacatgtagc gcgaggcttt cagggttgta
    53041 cgaaaagaag caaggtattt caaaggggag aagctccgcc tcaggtgacc gatggagtgt
    53101 ggttaaggta gcggtaaaag cgtgttaccg caatgttctg tcaccattgc aataatgcgt
    53161 atcgcttaga aaccattgcc ttaaccagga aagccggagt agatggacag ttctcagtac
    53221 gaaaaataag aaatgaccaa tctgccgacg cttgttaatg gttatatcct ggcgtcggca
    53281 gatcgcccag cagtcttatc tgaaaaaaga gagagccagg ttgtgtgaag ggatggatca
    53341 ccagtcaaaa cttttcacca gcgtcagctc gccagcatta cgcatcggta caataaatgt
    53401 ttcctgtttc tcattgaccg atccttcatc ggtgatcagc gtcagttggg cggtcgtcaa
    53461 ttccgtttcg ctgcgcccac catagtagtt gatatacacc tgatagcgcc cgtgaactgg
    53521 tgctggcatg gcgaaaatct cgggtccgta ccccgtcgtg acatccatat ccagtgcacc
    53581 actgtttttc agcacggtgt taccgtacca ggcgtgttcg ccatcgggcg tgacaacatg
    53641 gagatcgaga tcggtattgt ccgtatccca cgagagaacc agccgtaaac gtgcacgaat
    53701 agttcctgta cccggcgttg agtaaaactg cattttttgc cggctttgcc catccgggct
    53761 gatgacctgc acgctgttgc tgccttcagt gaaaatataa gggcgtgcaa atgaaccatc
    53821 ggattcaatt ctctgcggca tactggcacc gttgaccacc agccggccct gctgaacttt
    53881 acccgccgca gcataattct ttattttgcc gcggatctgc gctgagatat tttgatcatc
    53941 tgccatgttg acggacgacg ccgggtaatt gatcgattgt gaaaaactgg catcttcgcc
    54001 ctctgccgga tgccagccag agagcggtgc gtcaatctcg acttcctgca caccttcact
    54061 gtgagcgaca ggcgaaagtg ccaccagtaa caacggaaga aaaatctttc gcatagcatt
    54121 agtccattaa taattgtcgg gtcagcgttt cgatatagtt ctcatcaagc ccggtgggat
    54181 aaccatcaaa ggccaagtgc agatactcat gggttaaatc cagccgatcc tgaagcgtaa
    54241 agaagttacg aataaacagc cgtttctgct gccgatcggt ataggggaaa cctgagacta
    54301 aacgacagac cgcaaacacg tctggttcgt tgtaccccgt ctcgccttgt aatatacgcc
    54361 gccactgcgg catttttttc gccagccagg ctttcgcttt gggtaataac tggcaggttg
    54421 agcgtggagc tccccagcgg ctaaggctgt tgtcgggata agcaaacgcg agtatctggt
    54481 cgtaacgctc cccctgcccc gcctgggccg tggcttgccg ccaggaaagt gtcccttcgg
    54541 tagcgcgact gccatgataa tgaaccggat cgcctgcata gatgagctcc tgcgtccagg
    54601 cggtcatcgt tcgcgccccc gtggtcgctg gcgaagcaga aacacgctgc gtggcgctgc
    54661 tgtcggggat ggtcaggcaa tcaccttccc gattggcgtt ttgctgtaaa aaagtacgta
    54721 tcgccacagt catggctttc gctgcctcag gtggcgtact tttcgcctcg cgatccagaa
    54781 ctctggcaac gtactcttca cgatcgagat gcgactgcaa tttcagtttg cccttttcgc
    54841 tcagtaaggt cgtctcaccg tggctgacaa acgtaatgtg gttaccatta gtaaacgtca
    54901 cacgatagcg cccattaagt acgctgggtt ttactgccgt cgtactcttt tccgctgtga
    54961 ttttcttcag cggatagcgg gcaaacagct ccacctctac acattgccca ctggcaacct
    55021 gcgtcggcac tggcaaaacc cgattaagca ccgtcgcata acgggttagt accgttttgc
    55081 tggtgccact gccagtaacc cacagcggtg taccatcggt taaccagcca gcaaaaccac
    55141 cctgacggat ttcctgttta tcatcggcaa accagctcca ggttttcacc cgtacccgac
    55201 taccgagcat ggatgcgaca ccgattttcg cctcgtccag caccacatcc agtaagactt
    55261 cctgagcttt gttttgcgca ggtaacgtcg ctaatgaatc aagcagcgat ttaaccgtta
    55321 ccgaggtttc tggcttcagc gttgtaagag acgctaacca cgcaggtgcc tgacgctgtt
    55381 gccagtattg cccccacaca tctgcgccaa tatgcaaccg ctgcggcgca aaatagagcc
    55441 cacaggaacg taccagcgcg gtatcgcgcg ttatgctctc cccagcctga caacaataaa
    55501 cctcttccgg ggagtttccg cgacactggt agggctgctc cggttgatgg gtatcctcca
    55561 gccaggcgta aacatacagt ttccacaaac tccctaacgg cgtggtgagc gagtcaggta
    55621 atgctgagac tttggtcacg cctgatgacg acagctgata cagttgatcg tgctgcgcac
    55681 cgcgcagcac cagttgtaac ggcgtctcct ccgcaagcgt tggcagagta accaacgcca
    55741 atagccagac gattctgcgc cagttcactt atttcacctg catcccggtc cattcactcc
    55801 ctgccgcgac actttgctgc gcaggtgcat aggaacgcac ataacgagca ggaggcagaa
    55861 cgaattgccc tttttgcgag aagcgcagca aatggcggaa agtgaccgtt cccgtcagtt
    55921 ctttcaccgg caccatatag gccagttcgc ccatttcatt acgcgctttt tccagcaatt
    55981 gcccctgctg tttcgcggcg ttgggtttat tgactgaaat gccccatgtt gtgcgctcaa
    56041 cgtcagctcc cggcgggagc ggtacttcca cctgaccata gcgcagaact gcatcctgct
    56101 cgctggtaag cgtaatttca tcgagataca gcgcatcgct gtcaatctca ttgctggtca
    56161 ccggctgcag agtaaagctc atctcttctt caccggggat aagccgatac aactggcgtt
    56221 caacggtcac cggaatgtta ctttgttgag ccgtttttgc cggttcacgc cagcggacct
    56281 gcacatttgc ggcgataatt cgtcaccaaa agagagaatg tccggcacac cctaaccaac
    56341 ccaacgccag tcttcgcccc ctccagttaa cttatgttta gcccatgcgc ccgcaggcgc
    56401 aggcaaaaca actggaggca ttgtcgccat atatttcgcc agccagttca tggccagcgc
    56461 gcgctcaata gtggattgct cagcggtcaa accattaaaa tagcggccgt ctgggtagcg
    56521 tcaccaccgc tattgagcag cacaaccgtg tgcgccagcg gctgattgct gtttgccgct
    56581 ttatcccacg catattgctg aacctgtggc atcaccgtgg acttcaactg cgcttttttc
    56641 agcaacgtca tggttaagac gtttgccacc gcatctgcca gtggagattc cggtgtatcg
    56701 aggatcaggc tgtcattgat atcgcggatg tcttcctcag agaaatcttc atctttagtt
    56761 ccgcgccggg cgatagcttc atccccccct ttcaacaacg ttttgcacgg cagattcatc
    56821 tcctgtgccc acgccagcac cagcgcccga tgcaataacg gcatattatc cgcctgctcg
    56881 gcataactgt cgagcatatg ctgccagtat tccggttgtt gcgttacgcc gagcgcctgg
    56941 ctggcctgcc agtcggcgta ccatgcccat gccgtaagga aggcgtcacc attgccatct
    57001 tcaccccacc aggtaaagcg cgctccgggc cccgccagtt gcatcagccg cagacggtta
    57061 tcctgaatca tctgacgaat gtcgttagcg gcggcacttt gatgatcggc aagcgaacgc
    57121 caggcgagac tgagcgggat cagacggcta ccggtgttga tcaccccacc ccacggttca
    57181 tcgactaacg catcaagatt gttgcggaaa atctcctgcg gcgtttcact actttgtagc
    57241 cggatattac tcgcctgctc gggcaacatc agcgcgttct cgccaccgcc gagcatgaca
    57301 tttttctgct gttcaacggg ccagctgttg tccacaaaag acagttttgt gctaatgctg
    57361 tcctgcactt gcccattttg ttgcagttct gcacttaaca agccagattg ctgaatattc
    57421 tgcgtcagcg aaatataatt cgcccctttg tgcagcgtca gcgtctggcg catctcagcg
    57481 cctgcaaatt tagtcaccag cgccaccggt tcgttatcct gctgactgaa gataaacagt
    57541 cctgccgccg gtttgtcgcc cacgcgatac accgttggca tactccactt catgtagaga
    57601 tttttttccg aacgcagata agcacgcccc tgcccgacca gcccgtcgcc gttcatccca
    57661 cgcgcggtga tacgccagcg ggttaacgaa tcaggcatca ggaacgtgaa atacgctttg
    57721 ccttgtttat cggttgtgag tgacggcatc catgccgcgg tatccacctc ttcacgccgt
    57781 ggacgttcaa gcatttttac tcgccgctcg ctgcggttag tcgcgccagg cgcaaccggc
    57841 tcgctggaga gcgcctggtc atagctgata aacgacagac tggagctggt acgcacattg
    57901 ttacgcccca gcggatagaa aaatttgccg atattcggcg cgatttctgg ttgcagcgcg
    57961 tagatcattt catcgaccac gcctaccgtt agctgcgcag aaacaggttt acctttcagc
    58021 gacgaggtta at
//
LOCUS       DAJTSA010000016        55418 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000016 DAJTSA010000000
VERSION     DAJTSA010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.016"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="OO369_001027"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="OO369_001027"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR5981389.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OO369_001028"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OO369_001028"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR5981390.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OO369_001029"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OO369_001029"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR5981391.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="OO369_001030"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="OO369_001030"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR5981392.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="OO369_001031"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="OO369_001031"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR5981393.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="OO369_001032"
     CDS             7826..9559
                     /locus_tag="OO369_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR5981394.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="OO369_001033"
     CDS             9708..10559
                     /locus_tag="OO369_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5981395.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="OO369_001034"
     CDS             complement(10546..10887)
                     /locus_tag="OO369_001034"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5981396.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="OO369_001035"
     CDS             complement(10889..11767)
                     /locus_tag="OO369_001035"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR5981397.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="OO369_001036"
     CDS             complement(11733..14030)
                     /locus_tag="OO369_001036"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5981398.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="OO369_001037"
     CDS             complement(14081..14401)
                     /locus_tag="OO369_001037"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5981399.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="OO369_001038"
     CDS             complement(14416..15495)
                     /locus_tag="OO369_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR5981400.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..16907
                     /locus_tag="OO369_001039"
     CDS             15804..16907
                     /locus_tag="OO369_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR5981401.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16908..18305
                     /gene="ptsP"
                     /locus_tag="OO369_001040"
     CDS             16908..18305
                     /gene="ptsP"
                     /locus_tag="OO369_001040"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5981402.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="OO369_001041"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="OO369_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="OO369_001042"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="OO369_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20973
                     /locus_tag="OO369_001043"
                     /pseudo
     CDS             20366..20973
                     /locus_tag="OO369_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="OO369_001044"
     CDS             complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="OO369_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR5981403.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21999..24179)
                     /gene="katG"
                     /locus_tag="OO369_001045"
     CDS             complement(21999..24179)
                     /gene="katG"
                     /locus_tag="OO369_001045"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR5981404.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24508..25398)
                     /gene="metF"
                     /locus_tag="OO369_001046"
     CDS             complement(24508..25398)
                     /gene="metF"
                     /locus_tag="OO369_001046"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR5981405.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25747..28179)
                     /gene="metL"
                     /locus_tag="OO369_001047"
     CDS             complement(25747..28179)
                     /gene="metL"
                     /locus_tag="OO369_001047"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR5981406.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28182..29342)
                     /gene="metB"
                     /locus_tag="OO369_001048"
     CDS             complement(28182..29342)
                     /gene="metB"
                     /locus_tag="OO369_001048"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR5981407.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29619..29936
                     /gene="metJ"
                     /locus_tag="OO369_001049"
     CDS             29619..29936
                     /gene="metJ"
                     /locus_tag="OO369_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR5981408.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30120..30728
                     /locus_tag="OO369_001050"
     CDS             30120..30728
                     /locus_tag="OO369_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR5981409.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="OO369_001051"
     CDS             complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="OO369_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR5981410.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31204..33402
                     /gene="priA"
                     /locus_tag="OO369_001052"
     CDS             31204..33402
                     /gene="priA"
                     /locus_tag="OO369_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR5981411.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33558..34583
                     /gene="cytR"
                     /locus_tag="OO369_001053"
     CDS             33558..34583
                     /gene="cytR"
                     /locus_tag="OO369_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR5981412.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34675..35634
                     /gene="ftsN"
                     /locus_tag="OO369_001054"
     CDS             34675..35634
                     /gene="ftsN"
                     /locus_tag="OO369_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR5981413.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35727..36257
                     /gene="hslV"
                     /locus_tag="OO369_001055"
     CDS             35727..36257
                     /gene="hslV"
                     /locus_tag="OO369_001055"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR5981414.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36267..37598
                     /gene="hslU"
                     /locus_tag="OO369_001056"
     CDS             36267..37598
                     /gene="hslU"
                     /locus_tag="OO369_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR5981415.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37665..38591
                     /gene="menA"
                     /locus_tag="OO369_001057"
     CDS             37665..38591
                     /gene="menA"
                     /locus_tag="OO369_001057"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR5981416.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38684..39169
                     /gene="rraA"
                     /locus_tag="OO369_001058"
     CDS             38684..39169
                     /gene="rraA"
                     /locus_tag="OO369_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR5981417.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="OO369_001059"
     CDS             complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="OO369_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR5981418.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39925..40770
                     /gene="glpF"
                     /locus_tag="OO369_001060"
                     /pseudo
     CDS             39925..40770
                     /gene="glpF"
                     /locus_tag="OO369_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40810..42300
                     /gene="glpK"
                     /locus_tag="OO369_001061"
     CDS             40810..42300
                     /gene="glpK"
                     /locus_tag="OO369_001061"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR5981419.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42530..43540
                     /gene="glpX"
                     /locus_tag="OO369_001062"
     CDS             42530..43540
                     /gene="glpX"
                     /locus_tag="OO369_001062"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5981420.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43637..44383
                     /gene="fpr"
                     /locus_tag="OO369_001063"
     CDS             43637..44383
                     /gene="fpr"
                     /locus_tag="OO369_001063"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR5981421.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="OO369_001064"
     CDS             complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="OO369_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR5981422.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44843..45142)
                     /locus_tag="OO369_001065"
     CDS             complement(44843..45142)
                     /locus_tag="OO369_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR5981423.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45354..45794)
                     /locus_tag="OO369_001066"
                     /pseudo
     CDS             complement(45354..45794)
                     /locus_tag="OO369_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45895..46494
                     /locus_tag="OO369_001067"
     CDS             45895..46494
                     /locus_tag="OO369_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR5981424.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46602..47369
                     /gene="tpiA"
                     /locus_tag="OO369_001068"
     CDS             46602..47369
                     /gene="tpiA"
                     /locus_tag="OO369_001068"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR5981425.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="OO369_001069"
     CDS             complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="OO369_001069"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR5981426.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="OO369_001070"
     CDS             complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="OO369_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR5981427.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="OO369_001071"
     CDS             complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="OO369_001071"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR5981428.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="OO369_001072"
     CDS             complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="OO369_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR5981429.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="OO369_001073"
     CDS             complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="OO369_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR5981430.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52438..53136
                     /gene="cpxR"
                     /locus_tag="OO369_001074"
     CDS             52438..53136
                     /gene="cpxR"
                     /locus_tag="OO369_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR5981431.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53133..54506
                     /gene="cpxA"
                     /locus_tag="OO369_001075"
     CDS             53133..54506
                     /gene="cpxA"
                     /locus_tag="OO369_001075"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR5981432.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="OO369_001076"
     CDS             complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="OO369_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR5981433.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13491 a        15017 c        14166 g        12744 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ttgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttat attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgagcccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt tagtttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatcgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg tgtatgttcc
    20581 gatgtggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    20641 gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg cccggacagc
    20701 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca
    20761 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    20821 gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga cggtacgccg
    20881 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    20941 atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga agaaaaaatt
    21001 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    21061 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
    21121 gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat actggcaggc
    21181 aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag aatcgcactg
    21241 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    21301 gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa cgataataaa
    21361 tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    21421 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    21481 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    21541 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    21601 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc
    21661 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gcgcattccg
    21721 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    21781 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    21841 cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt tcctgcggca
    21901 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
    21961 agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg
    22021 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
    22081 ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa taccagatcc
    22141 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
    22201 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    22261 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca
    22321 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    22381 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    22441 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    22501 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    22561 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
    22621 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    22681 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    22741 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    22801 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    22861 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    22921 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    22981 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    23041 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
    23101 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
    23161 tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg gatcggcgtc
    23221 tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    23281 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctacg
    23341 tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc
    23401 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca
    23461 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag gcccatctcg
    23521 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    23581 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    23641 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
    23701 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    23761 tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    23821 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc catacgaata
    23881 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
    23941 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    24001 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
    24061 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
    24121 tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga cgtgctcatc
    24181 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    24241 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    24301 atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    24361 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
    24421 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    24481 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
    24541 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
    24601 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
    24661 ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt
    24721 catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc ccgggatgat
    24781 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    24841 gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag
    24901 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggatat
    24961 atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt
    25021 accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac cgttattcca
    25081 gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg
    25141 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    25201 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    25261 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    25321 cgaaacgtta atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
    25381 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    25441 cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    25501 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    25561 aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac taaccatgta
    25621 cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc tatgataatt
    25681 caatatattt cattagaaac catagacccg aaaacatgag taccgggcat tattaaattt
    25741 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    25801 gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc agcggctttc
    25861 gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt cacgcaccgc
    25921 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
    25981 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
    26041 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    26101 tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca gcttgcgcat
    26161 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
    26221 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    26281 cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga tcagatcgcg
    26341 cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc agtgacgccc
    26401 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg ctcccgccag
    26461 tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact gatcagcaag
    26521 ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg cgcgcatcca
    26581 caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg ctaacgcgcg
    26641 gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca cgcctgccag
    26701 cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga acagttccag
    26761 ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt tttctgcgcg
    26821 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    26881 caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc ctttcagttg
    26941 ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga ccatcgccac
    27001 cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt cgaggatttt
    27061 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc gatcgttatg
    27121 cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga tctctttatg
    27181 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    27241 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    27301 ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag aaactggctg
    27361 taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg cctcatccag
    27421 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    27481 ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga tttgtgtcgc
    27541 ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt tacggctgat
    27601 aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt gcagcaacgg
    27661 gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta aaaactcacg
    27721 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgcgc
    27781 cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa taccactgtc
    27841 gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc tatcggcttc
    27901 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    27961 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    28021 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact ccgccataat
    28081 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    28141 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    28201 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    28261 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    28321 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    28381 aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc cagttcaaaa
    28441 ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc
    28501 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
    28561 gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
    28621 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt
    28681 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
    28741 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
    28801 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    28861 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    28921 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    28981 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    29041 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
    29101 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
    29161 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    29221 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
    29281 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    29341 atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    29401 ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
    29461 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    29521 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtggggt
    29581 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    29641 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    29701 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    29761 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    29821 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    29881 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg
    29941 gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc ctacgtcata
    30001 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    30061 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    30121 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    30181 cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa gcgatccaac
    30241 tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac aaaaagccct
    30301 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg attgcccatg
    30361 gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    30421 aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    30481 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    30541 gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac ccgctggtcc
    30601 aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg gtagtttcac
    30661 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    30721 cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa
    30781 atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca
    30841 ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg
    30901 aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca agagcagcta
    30961 gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag
    31021 gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca
    31081 atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact
    31141 gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat
    31201 gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg
    31261 ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag
    31321 caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcactgag
    31381 ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc cgtctggcga
    31441 ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc
    31501 ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt
    31561 gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc caagcaacaa
    31621 caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc cacgctcgaa
    31681 tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa
    31741 acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg
    31801 aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc
    31861 tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa
    31921 aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg
    31981 caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct gcattccggt
    32041 ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga ggcggcgatt
    32101 gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt
    32161 gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca tgcccgcgac
    32221 ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc cgcaacgccc
    32281 gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacgcgc
    32341 cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg tcagaaggtg
    32401 caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat
    32461 caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg ccacgactgc
    32521 ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag
    32581 catttgcgct gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc
    32641 ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg
    32701 ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg
    32761 gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt gatcggcaca
    32821 caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg
    32881 gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca gctttacacc
    32941 caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct gcaaacgcac
    33001 catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgatgc ctttgccgaa
    33061 caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca tgtgattgtg
    33121 cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact gcgtaatctg
    33181 atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt tccggcactg
    33241 gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc ttcccgcgtg
    33301 cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc ggattcccgc
    33361 aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta cgatgcgagg
    33421 cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg
    33481 cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg
    33541 ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg
    33601 ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata
    33661 aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac
    33721 cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc
    33781 cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa
    33841 atcacggata tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct
    33901 ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc
    33961 cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat
    34021 ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc gcatttgatg
    34081 cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag
    34141 agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggaa
    34201 ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag
    34261 caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc catagcgatg
    34321 tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc
    34381 tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa
    34441 ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa
    34501 tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg
    34561 gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc
    34621 cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca
    34681 caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca
    34741 cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc
    34801 gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc
    34861 gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc
    34921 tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct
    34981 tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt
    35041 gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt tccgtggaat
    35101 gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta
    35161 gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag
    35221 acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accagcttct
    35281 actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc gcagtcgaag
    35341 ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa
    35401 aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag
    35461 acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc
    35521 tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat
    35581 cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct
    35641 caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc
    35701 tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc
    35761 atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg
    35821 tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg
    35881 cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc
    35941 tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac
    36001 ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg
    36061 acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg
    36121 ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg
    36181 cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat
    36241 taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg
    36301 aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc
    36361 gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa
    36421 atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta
    36481 agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa gtgggctacg
    36541 tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg aaaatggtac
    36601 gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc
    36661 tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag cagcaggaac
    36721 cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag
    36781 aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca
    36841 tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa
    36901 aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga
    36961 aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga
    37021 tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg
    37081 tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca
    37141 ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga
    37201 ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc cgcgttgaac
    37261 tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca
    37321 ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg
    37381 gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg
    37441 ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg
    37501 atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt
    37561 tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca
    37621 ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa
    37681 ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg
    37741 ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg
    37801 ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt
    37861 acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc
    37921 aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc gtcgtgctca
    37981 tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg
    38041 gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc
    38101 gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga
    38161 gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg
    38221 cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca
    38281 atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc
    38341 gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct
    38401 tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc
    38461 aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta
    38521 ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt
    38581 gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag
    38641 cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc
    38701 cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt
    38761 tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg
    38821 gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg
    38881 tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga
    38941 atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga
    39001 tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga
    39061 aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc
    39121 cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca
    39181 ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgaa
    39241 agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
    39301 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
    39361 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
    39421 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca
    39481 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct
    39541 cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt
    39601 atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt gccacacttt
    39661 tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc
    39721 gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct
    39781 cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg
    39841 ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa
    39901 acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt
    39961 gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta
    40021 aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg
    40081 gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc
    40141 attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca
    40201 caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta caatttattt
    40261 ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct
    40321 ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag
    40381 atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt
    40441 gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca
    40501 tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc
    40561 tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac
    40621 ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc
    40681 aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca
    40741 actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga
    40801 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    40861 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    40921 ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    40981 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    41041 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    41101 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    41161 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    41221 tggatcctcg accatgtgga aggctctcac gagcgtgcac gtcgtggtga attgctgttt
    41281 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    41341 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    41401 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    41461 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    41521 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    41581 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
    41641 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
    41701 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    41761 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    41821 tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    41881 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    41941 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    42001 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    42061 tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
    42121 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
    42181 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    42241 tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa
    42301 tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct tacgcgtctt
    42361 atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca cgccgcatcc
    42421 ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa taatcccacc
    42481 cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga
    42541 acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggtt
    42601 aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc gtattatgct
    42661 caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc
    42721 gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt
    42781 tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct
    42841 ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg agaagctgat
    42901 tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg
    42961 caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga ttctggctaa
    43021 accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat
    43081 tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt
    43141 gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga tccgcgcatt
    43201 agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga
    43261 gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca tcgaagccgg
    43321 taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg
    43381 tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg cgactaccga
    43441 aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca tccactatct
    43501 ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat cgattgagcc
    43561 ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc
    43621 aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg
    43681 gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca
    43741 atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt
    43801 taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt
    43861 aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc
    43921 tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg
    43981 tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag atcgcttcaa
    44041 aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat
    44101 gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga
    44161 aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg gggaactgga
    44221 aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc
    44281 acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga cgaaacattt
    44341 acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata
    44401 aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata tgccggcatc
    44461 aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg ctctttttgc
    44521 ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc
    44581 cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt caacaattga
    44641 agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca
    44701 atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttattcacc
    44761 agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt
    44821 aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca
    44881 tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc tccagatcct
    44941 taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg gcaagctctt
    45001 tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac
    45061 tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc
    45121 atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga
    45181 cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat ggattaccat
    45241 tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat tgtcatttgc
    45301 atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggttaatta
    45361 cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata tttattttcg
    45421 ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat caacgtcggg
    45481 acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt acccgccagc
    45541 agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag
    45601 cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa cgccgcggtc
    45661 tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac
    45721 aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat
    45781 tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac
    45841 aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag
    45901 ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac aacggcgcat
    45961 gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc
    46021 gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg
    46081 aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgcaagt
    46141 aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa
    46201 agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc gaaagcgaaa
    46261 gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac caaaacacaa
    46321 agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag
    46381 acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct
    46441 gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa
    46501 gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc
    46561 ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga
    46621 tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc
    46681 gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca
    46741 tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg
    46801 acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg aaagacatcg
    46861 gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg
    46921 aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg gttctgtgca
    46981 tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga
    47041 tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt atcgcttacg
    47101 aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc
    47161 acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca
    47221 ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca
    47281 tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta
    47341 aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca
    47401 cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc ctggatttcc
    47461 tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa
    47521 ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg cgcctcaggc
    47581 acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga
    47641 cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg
    47701 ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga gatatgaata
    47761 tgaaaatggt tttgcgtatg cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg
    47821 ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag
    47881 aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt
    47941 ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta tccggcattg
    48001 ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc
    48061 tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata
    48121 ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc
    48181 tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa
    48241 cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc gtttgctgat
    48301 ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa
    48361 ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgctac
    48421 ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta
    48481 gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc
    48541 gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt
    48601 atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc
    48661 gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat ccagaacttc
    48721 gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg
    48781 atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag agcttttcgg
    48841 attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt
    48901 cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg
    48961 cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata
    49021 ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg tcgcttgttt
    49081 acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct gtttccagtg
    49141 ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt
    49201 aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac
    49261 gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag
    49321 aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg
    49381 agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
    49441 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
    49501 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    49561 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    49621 gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    49681 ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga
    49741 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    49801 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    49861 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    49921 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
    49981 gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga tcgccacaat
    50041 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    50101 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    50161 ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca
    50221 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac
    50281 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
    50341 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg taacggtcta
    50401 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc attacttcca
    50461 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    50521 caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa ctttggaatg
    50581 caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
    50641 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    50701 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    50761 cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac
    50821 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    50881 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    50941 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    51001 cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    51061 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    51121 cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    51181 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa
    51241 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    51301 gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg
    51361 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    51421 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    51481 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    51541 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    51601 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    51661 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    51721 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
    51781 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
    51841 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
    51901 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
    51961 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
    52021 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
    52081 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
    52141 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
    52201 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
    52261 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
    52321 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
    52381 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
    52441 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
    52501 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
    52561 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
    52621 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
    52681 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
    52741 tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc gcactggagc
    52801 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
    52861 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
    52921 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
    52981 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
    53041 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
    53101 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
    53161 tcgccatctt ctggctgacg ctggtgctgg tgttgatgtt ggttttgatg ttacccaagc
    53221 tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg
    53281 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
    53341 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
    53401 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
    53461 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
    53521 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
    53581 gtagttctca atccgatttc attaacttac tgtttgaccg cccgctatta ctgctgattg
    53641 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
    53701 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
    53761 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
    53821 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
    53881 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
    53941 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
    54001 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
    54061 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
    54121 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
    54181 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
    54241 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
    54301 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
    54361 gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc
    54421 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
    54481 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
    54541 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
    54601 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    54661 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    54721 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    54781 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    54841 atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg
    54901 atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat
    54961 gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt
    55021 gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc
    55081 acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga
    55141 acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca
    55201 tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg aatcttgcct
    55261 gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt ccccattaga
    55321 ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc aggaactcaa
    55381 tagcaaaccg gacgaacgtt agttcatccg gctgtggg
//
LOCUS       DAJTSA010000017        53211 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000017 DAJTSA010000000
VERSION     DAJTSA010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.017"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(11..322)
                     /locus_tag="OO369_001077"
     CDS             complement(11..322)
                     /locus_tag="OO369_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR5981434.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="OO369_001078"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO369_001078"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="OO369_001079"
     CDS             701..1054
                     /locus_tag="OO369_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR5981435.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="OO369_001080"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="OO369_001080"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR5981436.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="OO369_001081"
     CDS             complement(2810..3280)
                     /locus_tag="OO369_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR5981437.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="OO369_001082"
                     /pseudo
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="OO369_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4119..4337)
                     /gene="hha"
                     /locus_tag="OO369_001083"
     CDS             complement(4119..4337)
                     /gene="hha"
                     /locus_tag="OO369_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5981438.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="OO369_001084"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="OO369_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR5981439.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /locus_tag="OO369_001085"
     CDS             complement(5283..8432)
                     /locus_tag="OO369_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR5981440.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="OO369_001086"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="OO369_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR5981441.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="OO369_001087"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="OO369_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR5981442.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="OO369_001088"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="OO369_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR5981443.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="OO369_001089"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="OO369_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR5981444.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="OO369_001090"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="OO369_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR5981445.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="OO369_001091"
     CDS             14911..15288
                     /locus_tag="OO369_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR5981446.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="OO369_001092"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="OO369_001092"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR5981447.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="OO369_001093"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="OO369_001093"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR5981448.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17379..17443
                     /gene="dnaX"
                     /locus_tag="OO369_001093"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18099..18428
                     /locus_tag="OO369_001094"
     CDS             18099..18428
                     /locus_tag="OO369_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR5981449.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="OO369_001095"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="OO369_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR5981450.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="OO369_001096"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="OO369_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR5981451.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="OO369_001097"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="OO369_001097"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR5981452.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="OO369_001098"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="OO369_001098"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR5981453.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23892)
                     /gene="aes"
                     /locus_tag="OO369_001099"
                     /pseudo
     CDS             complement(22933..23892)
                     /gene="aes"
                     /locus_tag="OO369_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24044..25348
                     /gene="gsk"
                     /locus_tag="OO369_001100"
     CDS             24044..25348
                     /gene="gsk"
                     /locus_tag="OO369_001100"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR5981454.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25481..27157)
                     /locus_tag="OO369_001101"
     CDS             complement(25481..27157)
                     /locus_tag="OO369_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR5981455.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="OO369_001102"
     CDS             complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="OO369_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR5981456.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28833..30485
                     /gene="ushA"
                     /locus_tag="OO369_001103"
     CDS             28833..30485
                     /gene="ushA"
                     /locus_tag="OO369_001103"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5981457.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="OO369_001104"
     CDS             complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="OO369_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR5981458.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31205..31999)
                     /locus_tag="OO369_001105"
     CDS             complement(31205..31999)
                     /locus_tag="OO369_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR5981459.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32137..32478
                     /locus_tag="OO369_001106"
     CDS             32137..32478
                     /locus_tag="OO369_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5981460.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32693..35197)
                     /gene="copA"
                     /locus_tag="OO369_001107"
     CDS             complement(32693..35197)
                     /gene="copA"
                     /locus_tag="OO369_001107"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR5981461.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35459..36391
                     /gene="glsA"
                     /locus_tag="OO369_001108"
     CDS             35459..36391
                     /gene="glsA"
                     /locus_tag="OO369_001108"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR5981462.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36394..37686
                     /locus_tag="OO369_001109"
     CDS             36394..37686
                     /locus_tag="OO369_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5981463.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37811..38218
                     /gene="cueR"
                     /locus_tag="OO369_001110"
     CDS             37811..38218
                     /gene="cueR"
                     /locus_tag="OO369_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR5981464.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38219..38677)
                     /locus_tag="OO369_001111"
     CDS             complement(38219..38677)
                     /locus_tag="OO369_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR5981465.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLCWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38674..39591)
                     /locus_tag="OO369_001112"
     CDS             complement(38674..39591)
                     /locus_tag="OO369_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5981466.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39737..40414
                     /gene="fetA"
                     /locus_tag="OO369_001113"
     CDS             39737..40414
                     /gene="fetA"
                     /locus_tag="OO369_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR5981467.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40401..41180
                     /gene="fetB"
                     /locus_tag="OO369_001114"
     CDS             40401..41180
                     /gene="fetB"
                     /locus_tag="OO369_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR5981468.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="OO369_001115"
     CDS             complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="OO369_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR5981469.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="OO369_001116"
     CDS             complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="OO369_001116"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5981470.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="OO369_001117"
                     /pseudo
     CDS             complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="OO369_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43550..44236
                     /locus_tag="OO369_001118"
     CDS             43550..44236
                     /locus_tag="OO369_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5981471.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44233..46648
                     /locus_tag="OO369_001119"
                     /pseudo
     CDS             44233..46648
                     /locus_tag="OO369_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="OO369_001120"
     CDS             complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="OO369_001120"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR5981472.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48524..49450)
                     /gene="allS"
                     /locus_tag="OO369_001121"
     CDS             complement(48524..49450)
                     /gene="allS"
                     /locus_tag="OO369_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR5981473.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49680..50162
                     /gene="allA"
                     /locus_tag="OO369_001122"
     CDS             49680..50162
                     /gene="allA"
                     /locus_tag="OO369_001122"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR5981474.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50240..51055
                     /gene="allR"
                     /locus_tag="OO369_001123"
     CDS             50240..51055
                     /gene="allR"
                     /locus_tag="OO369_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR5981475.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51145..52926
                     /gene="gcl"
                     /locus_tag="OO369_001124"
     CDS             51145..52926
                     /gene="gcl"
                     /locus_tag="OO369_001124"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR5981476.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52939..>53211
                     /locus_tag="OO369_001125"
     CDS             52939..>53211
                     /locus_tag="OO369_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR5981477.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12831 a        13689 c        14017 g        12674 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtgcc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gaactatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttactgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattcttgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaatcg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gttcttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttcaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagcagg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgcgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgaagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcacagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacctgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgccactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcacca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgt tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caacatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacca ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccacttc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggaaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg aggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaaga
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactat gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacag ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgtttgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cctggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggtgaag ctagcggcag aagccattga gcgcgacccg tgggcggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggtgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca agcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgat atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tggaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaatc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagcag gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctt atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc tccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctagtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactccgccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    23461 ataacaacaa gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag
    23521 ggtgtaatta ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    23581 atcgtgggta tcgagattgc cgagaataaa accgcctcca tgcaaataaa atagcgtcgc
    23641 cgggctatct ggctgcggac aaaagagacg tgtttccacc tgcccatatt ttgttggaac
    23701 catgtaagct ctggttgcca tttctggagc gcccgcattc cagaatcggc gctcaagcgt
    23761 gtaatactgt cgttgctcag caatcgttcc cgttgcgggc caggacggta aatccggctg
    23821 aagagtattc acaacggtct tcatttcagc agaaataagg tccagaacag gtagtttgtt
    23881 ttccggcttc atacaaaaac tcctttcaaa ttacgtcatt gtaaggaacc actgccatga
    23941 aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc tcgcgcgctc ccgtcagatt
    24001 gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca accatgaaat ttcccggtaa
    24061 acgtaaatcc aaacattact tccctgtaaa tgcacgcgat ccgctgcttc agcaattcca
    24121 gccagaaaac gaaaccagcg ccgcctgggt cgtaggtatt gaccagacgc tggtcgatat
    24181 tgaagcgaaa gtggatgatg aattcattga gcgttatgga ttaagcgccg ggcattcact
    24241 ggtgattgag gatgacgtag ccgaagtgct ttatcaggag ctaaaacaga aaaacctgat
    24301 tacccatcag tttgcgggtg gcaccattgg taacaccatg cacaactact cggtgctcgc
    24361 ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat attgaaattg gcagttatgc
    24421 ctatcgttac ctgtgtaaca cttccagccg taccgatctt aactatctac aaggcgtgga
    24481 tggtccgatt ggtcgttgct ttacgctgat tggcgagtcc ggggaacgta cctttgctat
    24541 cagtccaggc cacatgaacc agctgcgggc tgaaagcatt ccggaagatg tgattgccgg
    24601 agcctcggca ctggttctca cctcatatct ggtgcgttgc aagccgggtg aacccatgcc
    24661 ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat aacgtaccgg tggtgctgac
    24721 gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg tggcagcaat tcctcaaaga
    24781 ccacgtttct atccttgcga tgaacgaaga tgaagccgaa gcgttgaccg gagaaagcga
    24841 tccgttgttg gcatctgaca aggcgctgga ctgggtagag ctggtgctgt gcaccgccgg
    24901 gccaatcggc ttgtatatgg cgggctttac cgaagacgaa gcgaaacgta aaacccagca
    24961 tccgctgctg ccgggcgcta tagcggaatt caaccagtat gagtttagcc gcgccatgcg
    25021 ccacaaggat tgccagaatc cgctgcgtgt ctattcgcac attgcgccgt acatgggcgg
    25081 gccggaaaaa atcatgaaca ctaatggagc gggggatggc gcattggcag cgttgctgca
    25141 tgacattacc gccaacagct accatcgtag caacgtacca aactccagca aacataaatt
    25201 cacctggtta acttattcat cgttagcgca ggtgtgtaaa tatgctaacc gtgtgagcta
    25261 tcaggtactg aaccagcatt cacctcgttt aacgcgcggc ttgccggagc gtgaagacag
    25321 ccaggaagag tcttactggg atcgttaagt tatcgtcggt tcgtaggcca gataaggcgt
    25381 tcacgccgca tctggcattt ggctctcgat gcctgatgcg acgctggcgc gtcttatcat
    25441 gcctacatat ttttcatatt ttacatccgg caaccaccgt ttaccccgtc accacttcac
    25501 ccgccggtgg cgtttccagc agttccagca tagtacgggc aatttcacgt tcacccatca
    25561 ctacctgatt cgcgccacgt tcggtgatat acgtcacttc atcgtcataa tgggcgcggg
    25621 cgataatctc aatatccggg tttttcgcgc gggcagacgc cacaatctca cccgcttcat
    25681 aaccgttggg gatcgtcagg atcagccatt ttgcacattc cagatgtgcc agttgcataa
    25741 tttcttcgtt cgctgcattg cccaatacag cgcggacccc acgctctcgc agctcatcaa
    25801 cacgggttcg tgacgtctca atcaccacca gcggaatatc agaggcgagc aatttctccc
    25861 ccagcaggct gcctacacga ccgtaaccca ccaatagcgc atggttgcaa atatccactg
    25921 ggatctgttt ctcttcttcg attgcctctt ccagcgtctg ctcttccagc gtttcggtct
    25981 tcgccagata tttctccagt agtgcgaaca gtaccgggtt gagcataatc gacaggatcg
    26041 cccctgccag taccaggttt tgtccggcct gcggcagtaa attcaatgcc attcccagtc
    26101 ccgccaggat aaacgcgaac tcaccaatct gcgccaggct ggcggcgatg gttaatgccg
    26161 tacgttggga gtgaccaaac agacggacaa ggaacagggc cgcgaccgat ttaccaaaca
    26221 gaataattgc cagcgtcgcc agcactgcca gcggttgctg aatcagaatt aacggatcaa
    26281 acaacatccc gactgagaca aaaaacagca ccgcaaacgc gtcgcgcaat ggcaacgtat
    26341 cgtgggcggc acggtgactc aattcagact cgttcagtac catcccggca aagaacgcac
    26401 cgagtgcaaa ggagacatca aacagctcta ccgcaccaaa ggcaatccct aacgccagcg
    26461 ccagcactga cagggtaaac agctcgcgag aaccggttgc cgcgctgcgt gccataatcc
    26521 acggcaccag acggcgacct accagcatca taatggcgat aaatgcgatc accttgccga
    26581 tggtgatccc catatcgact gcaagagtgg caaagcccac atcgccctgt tccatcattc
    26641 ctgccactgc gggcagcaac accagcgtca gaaccatcac caggtcttcc acaatcaacc
    26701 aaccgatggc gatttgccca cgctgactgt ctattaattg ccgttcttca agtgcgcgca
    26761 gtaacaccac ggtactggcg gtggaaagac ataagccgaa cacgataccg gtcattaacg
    26821 accagcccag cacggcggag agcgccatac ccagcagcgt cgccacggct atctgggcga
    26881 tcgcgccggg aatggcgatg gactttaccg ccatcaaatc cttgagcgaa aaatgcaaac
    26941 cgacgccgaa catcagcaaa ataacgccca gttcagccag ttccggcgca agcttggtat
    27001 cggcaacaaa gcccggagtg aatggtcctg ccagcacacc cgctaacaga tatcccacca
    27061 gaggagaaat acgtagttta ttggccagca tgccgaggat aaaggcgagc acaaggccgc
    27121 caacaatggt ggtgataagc ggggtggcgt gatgcattcc gtctcctttt cctggtggtt
    27181 attgtccatt tttggctggg aaaaccaaaa ttacaggtaa tagtttatga caatttcatt
    27241 gatgatgttt atgaataatt gttgaatttt gcagaaaaat ggaattagct gcaaaaaaag
    27301 cacggttcgg aaaacagaag gcggtttaac agaggaaagg cttatggcga ctgtaaaacg
    27361 atgcagccaa agtttgcctt tggctgcaat gaaatcagtc tttatgccgg ttatcaggca
    27421 ggaatatggt caacatcccc aatagtggca ggaaagcaca gattttatag actaactcga
    27481 tgctggtgtg atcggcgata agccccagaa ctgccgctcc cagacctccc atgccgaaag
    27541 caaaaccgaa aaagagtcca gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt
    27601 agaccagaat ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc
    27661 ccgtccagtg caggcgggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag
    27721 agccccaaat cacatatttc cgtccaattt tatcccctac aggcccgccg atcaccgtac
    27781 ctgccgcaac ggcaaacagg aaggcaaaca gatgaagctg ggcattctgg atagataatc
    27841 cgaatttttg catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt
    27901 tcgagaaaat gaggattaac agaatgctga ctgccagtac cactttgttg cgcggcagtg
    27961 gattgattat cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac
    28021 ggctaatttg cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt
    28081 tgcctttgcc ataaggcgcg ataatcaccg ccgccagcaa gggtcccagg gaactgccaa
    28141 agttgccgcc gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac
    28201 gggctacgcg agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgctg
    28261 ccagcagaac tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa
    28321 agcacatgcc aattggcaac gaccacggca tcggatattt atcggtccag tagccgacca
    28381 ctggttgcag tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa
    28441 atgtcagaga aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca
    28501 tgtcgttcag cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct
    28561 tggtcgttga cgcagccgcg cccgccacag gctgggtttg ttcactcatt gccataggaa
    28621 agtcactttt tcagggttgc gatgtaaaga atgatcttat ttgtgattat tacccgacta
    28681 acatacctgt atgcgtcgtc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg
    28741 gatgcagatt tatcttcacc ggacgcagac ttgtctatga tgttgcgtca tactattttt
    28801 caacacgttg aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt
    28861 tagcgctgtt aaccacattt acactggcga gtgaaactgc tctggcgtat gagcaggata
    28921 aaacctacaa aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg
    28981 aatatggcga atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg
    29041 ttgcggctga aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc
    29101 ccgagtctga cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg
    29161 acgcgatggc gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg
    29221 aaaagtgggc caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc
    29281 gcctgtttaa accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gttattgggc
    29341 tgacaaccga tgacacagca aaaattggta acctggaata cttcactgat atcgaatttc
    29401 gtaagcccgc cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag
    29461 acattattat cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg
    29521 caccgggcga tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg
    29581 gtggtcactc gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg
    29641 tgccgggtac gccatgcaaa ccagatcaac aaaacggcat ctggattgtg caggcgcatg
    29701 agtggggcaa atacgtggga cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg
    29761 ttaactacca gctgattccg gtgaacctga agaagaaagt gacctgggaa gacgggaaaa
    29821 gcgagcgcgt gctttacact cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat
    29881 caccgttcca gaacaaaggc aaagcgcagc tggaagtgaa aataggcaaa accaatggtc
    29941 gtctggaagg cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc
    30001 tggcagccca aatggatcgc actggtgccg actttgcggt gatgagcgga ggcggaattc
    30061 gtgattctat cgaagcaggc gatatcagct ataaaaacgt gctgaaagtg cagccattcg
    30121 gcaatgtggt ggtgtatgcc gacatgaccg gtaaagaggt gattgattac ctgaccgccg
    30181 tcgcgcagat gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg
    30241 cgaaagacgg caaactgaac gaccttaaaa tcaaaggcga accggtcgat ccggcgaaaa
    30301 cttaccgtat ggcgacatta aacttcaatg ccaccggcgg tgatggatat ccgcgccttg
    30361 ataacaaacc gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata
    30421 tccagaaaag ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc
    30481 agtaatccga aagtgccgga tgtttgcatc cggcacaatg cttaatcgcg gcgggcgata
    30541 tcagcaaatt tggcatcgag gatctttgcc agatcgcctg ccgccagttc gatatccagt
    30601 ccgcgcttgc cgccggaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc
    30661 gttggcagac gttttttctg ccccagtggg ctaatccccc caaccaggta tcccgtcgaa
    30721 cgctgcgcga ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact
    30781 tttttaagat ccagttgacc tgcgaccggc gtaacggcca cggcaaggtg tttcatatca
    30841 ccgttcactg ccaccagcag cgttttgtag acctgatccg gattcaaacc taattttttg
    30901 acgacttcat cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac
    30961 gaaatcttgt ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa
    31021 acaaaaaaac gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct
    31081 aagagtattg gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg
    31141 acggcataat aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat
    31201 ttttttagcg caacatttgc ggcaaattcc cttctccata caggtgtagt gcaccgaccg
    31261 cgaccacata tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct
    31321 gatgcatcag cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atatcattat
    31381 tttgcggcgg tgcattcagc caccagctca tcatttgttg cagcaaccgt gcgttggtat
    31441 gccagtgggt cagcgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca
    31501 tggcaatctg gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg
    31561 cctgcaatag ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg
    31621 tcgcctgaag aaccatcgcg atttgccaca gcggttgggt agaaaagagt gagggagaaa
    31681 tgcccatctc ctggctaatg tgctgcaggt tttggagttg ctcctcgcta atgcgctctt
    31741 ccagcgcctc gcaggcaggc aaattagcaa aaggcgtatc gctggtggaa acatccgcct
    31801 cgacgatcag cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca
    31861 tatcgtggct acccatatga atactgccaa tcagatgaaa atggcgattg ccggggaggg
    31921 tgatatcgat ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta
    31981 cccggtacaa cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggtgag
    32041 ggatggcgca aatggagttg gggacttgtt taatgtgttt gtatgattca gtatgttctt
    32101 gcatcgctat tcacagggaa gcaacagtta aaaaccatga aacaggcaac aagaaaaccg
    32161 acgacacccg gagatattct tctctatgaa tatctggaac cgctcgattt gaaaatcaat
    32221 gagttagcag agttgctgca tgttcatcgt aatcgcgtca gtgcactgat caataacaat
    32281 cgtaaactca ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat
    32341 tttaggctaa acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc
    32401 caggaagaat tgggacggat tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt
    32461 gcaaaaaagg tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttc
    32521 acgccgcatc cggcatccga gcaccattgc ctgatgcgac gcttacgcgt cttatcaggc
    32581 ctacaaacct gtgcctgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca
    32641 tgcgtcgatg ccaaatgcgc cgccctaaag cagcgcatcc gcaatgacgt acttattcct
    32701 tcggtttaaa ccgcagcaac cggttggcgt tacttactac ggtaattgac gagagcgcca
    32761 ttgccgctcc ggcaactacc gggttaagca gtgttccagt aaacggccac aaaataccgg
    32821 cggcgaccgg aataccgatg ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt
    32881 tgcgcagcgt tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg ctatggcgca
    32941 tcagggtaat cgccgcggtt tcaatggcaa catcactgcc gccacccatc gcaatgccga
    33001 catccgcctg ggccagcgct ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac
    33061 gtccttcact ttgcagacgt ttgatcgctt cggctttacc gtccggcagc actccggcaa
    33121 tcacttcatc aattccagct tctttggcga tcgcattggc ggtggttggg ttatccccgg
    33181 tcaacatcac cagacgatat cccgctttat gcaggcgttg cagcgccgcc acgctatcac
    33241 tacgcaacgg atcgcgtaca gccagcaggg ctactgcttt cccgtcaatc gccagcagca
    33301 caggcgttgc tccttgcgat gcctgagctg aaatatcggc ttcaatcgct ttagtatcga
    33361 cttgctgatc atttaacaac gcctgattac ccagcaataa cgcatgacct tctgcttcac
    33421 cgctcacgcc cagcccgcgc aatgtgcgga aaccgttgac ctgcggtagc tgcatatcgc
    33481 ctgctttatc aaggatcgct cgtgccaatg gatggctgga accttgctcc agcgccgccg
    33541 ccagacgcag cgcctgcgct tcgtcaacat cagcaaatgt tttcactgcg acaacctgcg
    33601 gcttcccttc ggtcagcgtc ccggttttat cgaacacgac ggtatcgagt gtactggcgc
    33661 gttgcagcgc gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc ccgacgccgg
    33721 aaataatcga catcggcgtc gccagtccca gcgcacacgg gcaggcgata atcagcaccg
    33781 tagtggcaat caccagggta tagacaatct gcggtgccgg accaaagaaa taccagattg
    33841 ccgcactgac aagcgcaata accactacta ccggcacaaa tatggctgag attttatccg
    33901 ccagctgacc gatttctggc ttgctgctct gggcctggcg caccatgcga atgatccttg
    33961 atagcgtggt atggctgcca accgcactgg cacgaaacag cacactgccg tcctgtacca
    34021 ctgtcccggc atggacgcta tcgccttcgc ctttttgctg cgggattggt tcgcccgtca
    34081 gcatcgcttc atccagccat gcttcgccct gggtaatctc gccatctacc ggcacgcgat
    34141 cgccggtcgt caggcgcagc aacatacctg gctgcacttc tgccagaggc acgctttttt
    34201 caccttcgtc agtaaccagg cgcgccgtcg gtggggttaa atcgagtaat ttctccagtg
    34261 ccttagaaga acgctgacgt gcgcgtgctt ccagcatatg gccgagattg atcagaccga
    34321 taatcatcgc actggcttca tagtaaagat gtcgcgcttc catcgggaac cactgcggcc
    34381 acaggttgac gctcatcgaa tagagccacg ccacgcccgt tcccagcgcc accagcgtat
    34441 ccatcgtcgc tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa tggccgctgg
    34501 cgaaaaccat caccgccagg gttatcaggc cgataaccaa ccacaggctg cggttgtcgg
    34561 cggtgactat catgttatcg ccgatcatcc cccagaccat caccgggata cccaccgcca
    34621 gtgcgacaat tgcctgccag cggaagcgct tcatcgtagc gacggcggtt tcttgctggc
    34681 gttcgcggcg tttcgcgtca tcttcaatcg cttccgcgcc gtagcccgct ttttccaccg
    34741 cctgcactaa atcttgtggg gaggcactgc ccatcaccag cgcagtacgc tccgccaggt
    34801 ttacccgtgc ctgagtgacg cccggtacgc tttgcagcgc attttgtacg cgggtgacac
    34861 agctggcgca gctcatgccg ctcagcagca actgctggct gtcatcgtca tcggcggtcg
    34921 ctgccggaag cgcctcagaa accgctgtca gtgcttccga cgggattgat gactccgcca
    34981 gcggtttagc ctttgggtgg cttacagatg cgtcataacc cgcttgtttg atggtttcga
    35041 tcagctgttc tgcactggca gtcccggtaa cgtgcgcttc agtgatagac acatccgctt
    35101 gttccacatc cgggcgctgt tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg
    35161 acaggccgtc caggttcagg tcgatagttt gtgacataaa acactccttt aagacagttt
    35221 tgactggctg tgataaaggt taaaccttcc agcaagggga aggtcaagaa attaataaac
    35281 caggcgggta aaagtccgta aagattaaaa aatcggctcg atttgcatca ggattagaca
    35341 tttatttctt tgttttcctg tagttaagtt gcgggtgcta agttaaatca ggatgcctga
    35401 aaatcggcac tggggtgagg aattacctcc cgcatctata aaaaggagtt aacaaaagat
    35461 gttagatgca aacaaattac agcaggcagt ggatcaggct tacacccaat ttcactcact
    35521 taacggcgga caaaatgccg attacattcc ctttctggcg aatgtaccag gtcaactggc
    35581 ggcagtggct atcgtgacct gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg
    35641 ctttgcactg gaatccatct ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg
    35701 cccgcaggcg gtacaggaca aaattggcgc tgacccgacc ggattgccct ttaactcagt
    35761 tatcgcctta gagttgcatg gcggcaaacc gctttcgcca ctggtaaatg ctggcgctat
    35821 tgccaccacc agcctgatta acgctgaaaa cgccgaacaa cgctggcagc gaattttaca
    35881 tatccaacag caactggctg gtgagcaggt agcgctctct gacgaagtca accagtcgga
    35941 acaaacaacc aacttccata atcgggccat tgcctggctg ctgtactccg ccggatatct
    36001 ctattgtgat gcaatggaag cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa
    36061 tactgttgaa ctggcaacgc ttggcgcgac gctggcggcg ggtggtgtga atccgttgac
    36121 gcataaacgc gttcttcagg ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga
    36181 agggctgtat ggtcgctccg gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg
    36241 tgtaggtggc ggtattctgg cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc
    36301 accgctggac gaagaaggca atagtgttcg cggtcaaaaa atggtggcat cggtcgctaa
    36361 gcaactcggc tataacgtgt ttaagggctg atcatgatga acacggaagg taataacggt
    36421 aacaaacccc tcggtctatg gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg
    36481 gggatcttcg cgctgctggg gcaggctgcg ttgctaatgg aagcctcgac ctggggcgcc
    36541 tttgcttttg gcggtattgt ggctatgttt tccggttatg cctatgcgcg tctgggggcg
    36601 agctatccca gcaatggcgg tattatcgac ttttttcgtc gcggattagg caacggcgtc
    36661 ttttcgctag cgctctcgtt gctatacctg ttgacgctgg cggtgagcat cgccatggtt
    36721 gcccgtgctt ttggcgctta tgccgtgcag tttttgcatg aaggcagcca ggaggagcac
    36781 cttattttgc tctacgcgtt ggggatcatt gcggtgatga cgcttttcaa ctccttaagc
    36841 aaccatgcgg tagggcggct ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta
    36901 ttgctgatta ttgccggtgt ctggtcgctg caaccggcgc atatttccgt ctctgcgccc
    36961 cccagctccg gtgcgttctt ctcctgtatt gggataacct tccttgccta tgcgggcttt
    37021 ggcatgatgg cgaacgcggc ggataaagtg aaagatccgc aggtcattat gccacgggcg
    37081 tttctggtgg cgattggcgt taccacgttg ctttatatct cgctggcgct ggttttgctt
    37141 agcgatgtat cggcattaga gttagaaaaa tatgctgata ccgccgtagc gcaggctgct
    37201 tctccgctgc tcgggcatgt gggttatgtg atcgtcgtca tcggcgcttt actggcgacg
    37261 gcttcaggca ttaacgcgaa cctgttcgcc gtgtttaaca tcatggacaa catgggcagc
    37321 gaacgcgaac tgccgaagct aatgaataaa tccctgtggc ggcagagtac ctggggcaat
    37381 atcatcgtcg tggtgttgat aatgctgatg acggcggcgc taaatttaga ctcactcgcc
    37441 agcgttgcca gcgccacctt tttgatctgc tatctggcgg tgtttgtggt ggcgatccgc
    37501 ctgcgtcatg atattcacgc ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg
    37561 ttggtgatcg ttggctttat ctacagcctg tggtcccagg gcagccgtgc gttgatatgg
    37621 attattggtg caatcttact cagccttatt gtggcaatgg tcatgaagcg taataaaacc
    37681 gtataacacc tctctgtgcg cagtacttcc tgtattattg tggtggcggt cgatattcgc
    37741 actggcaaaa aaacgtgctt gaatatctgt tgaaaccctt taacaaagca caggaggcgt
    37801 tgcgcgaacg atgaacatca gcgatgtagc aaaaattacc ggcctgacca gcaaagccat
    37861 tcgcttctat gaagagaagg ggctggtgac gccgccgatg cgcagcgaaa acggttatcg
    37921 cacctacacg cagcagcatc tcaacgaact gaccttactg cgccaggcac ggcaggtggg
    37981 ctttaacctg gaagagagcg gcgagctggt gaatctgttt aacgacccgc agcggcacag
    38041 cgccgacgtc aaacggcgca cgctggagaa ggtggcggag atcgaacgac acattgagga
    38101 gctgcaatcc atgcgcgacc agctgctggc actggcgaat gcctgccctg gcgatgacag
    38161 cgccgactgc ccgattatcg aaaatctctc cggctgctgt catcatcggg cagggtgatt
    38221 aagacgagac ggctcggata tgtagggtta tcccttctat cgcaatgact tcaacatgcg
    38281 tacctgcacc gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat
    38341 gaccgcgccc gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc
    38401 cgcgctggtt taaatgacta tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt
    38461 tccaccacag ccaggcggcg agcagcgtca ggatggcaaa cattacccct tgccactccc
    38521 aacacagcgg caccagccag accaccaggc cggtaatcac cgctgccacg ccgctccaca
    38581 acaaataacc atttccgccc agcatctcgg ctgccagcag caacccgccg agactgagcc
    38641 agaaaatatg tggatgaacg accattaact ccatcatggc tgagtccgtt tgttggcgct
    38701 gtctttcacc agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg
    38761 catcatcact actttgctgt tactggagga accgatctgc tgtaacgctt cggtgtattt
    38821 ttgcgctacg aagtagttca ccgcctgaat atcaccggag gcgatagctt cagacaccat
    38881 tttggtggcg cgggcttctg cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc
    38941 cgactgacgt tcgccttccg ctttcaggat ttgcgactgt ttttcacctt cggctttgag
    39001 gatttccgcc tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc
    39061 tttcatctgc gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc
    39121 aatacgggtg actttaatcc cccacgggtt ggtggcttca tcgacaatac gcagcaggcg
    39181 tgagttgatg ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt
    39241 acggatgtta gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataggc
    39301 agcgcgtggc gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt
    39361 atctttcgag ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt
    39421 gcgaccaatg cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt
    39481 atagcgacca aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc
    39541 gccgacaatg accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac
    39601 ctcctgttgt accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga
    39661 ttactgaaag agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca
    39721 gaaaaaggga acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata
    39781 tctggcgggt gatacgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt
    39841 taagttaatt accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc
    39901 attgatcagc ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa
    39961 accagaaatc taccgccagc aagtctctta ctgcgcccag acgccgacgc tgtttggcga
    40021 tacggtatac gataatctga tctttccctg gcagatccgt gaccagcagc ctgacccggc
    40081 catttttctc gattttctcg aacgcttcgc tttgccggac agcattttga cgaagaatat
    40141 cgccgagcta tctggtggtg aaaaacaacg catctcattg attcgtaacc tgcaatttat
    40201 gccgaaggtt ttattgctgg atgaaataac cagtgcgctg gatgaaagta ataaacataa
    40261 cgtcaatgag atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac
    40321 acacgataaa gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgccgg
    40381 agaaatgcag gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg
    40441 cattaatgct ggtggtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga
    40501 aagatattct atggagcgtc gggcgagcga taattcagct gattattgtc ggctatgtgc
    40561 tgaagtatat tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct
    40621 gctttaatgc ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct
    40681 catcgtttat tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag
    40741 gggcgattga atttatcccg atgcaggtga tccctgtcgc cgggatgatt gccggtaacg
    40801 ccatggtagc ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc
    40861 aacagattca ggagaaactg agtcttggcg cgacgccgaa gcaggcttca gcgatattga
    40921 ttcgcgacag tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct
    40981 tagtgagttt accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg
    41041 cgattaaata tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca
    41101 ttattgcctg ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga
    41161 ctcaattgaa gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt
    41221 tcatgtgccg aacaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg
    41281 acgccagtgc atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct
    41341 gaccgtctgc ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt
    41401 cattgcgccc aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg
    41461 gattctccgc cacctgctgt tgcaactgtt gaatttccgg cttatcagcc gcctgcttca
    41521 gcagttcgat ttgcgctacc agcccctggt agcgggtgtc ctgatcctgc aacggaatgg
    41581 ttttcagcac cgcttccgca tcttcagaac ggttcagcgc aatcagcgtt tctgccagca
    41641 gcaggccgat ctccccgttc tgattcgaca actgccaggc gtctttcagc aatggcaggg
    41701 catcggtgta attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt
    41761 cgcgcggcag cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga
    41821 agccatctac cggttgcccg ttctggaaca gatacacggt cggaatcgcg cgcagaccaa
    41881 actgggcggc aatcatctgc tccgcgtcgc agtccagctt cgccagaata aattgcccgt
    41941 tgtactgcgc cgcgaggctt tccaggatcg gggttaactg caaacagtgc tggctacgtt
    42001 cagaccaaaa atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt
    42061 tagattcgtt aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata
    42121 ttttctttga catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca
    42181 tcacgcgccc aggcagcagg cgctcgagca ccattactgc ccaggtcacc agcgttaccg
    42241 gatagcgcat cttcggcttc tcgctaataa aagcatggcg cactttgtcg accaccgctt
    42301 ccggtcccaa cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg
    42361 tctggttgac gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt
    42421 taattccgct gtggcgtagc tccatgcgca gcgcgtctga ccacgcctcc agcgcatatt
    42481 tactggccgc gtaagcgcca cgacccggcg tggagattaa tcccatcacc gatgatgtca
    42541 tcacaatacg cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt
    42601 gtgcgccgaa aaagttggcg gaaaactgct gttccatctg cgcacggctg atggtggaaa
    42661 gggggccata catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca
    42721 gggcgatcac ctcgtctgct gcgcgattaa cactttctgg tgaatccaga tcgatcaaca
    42781 cgccggtaaa tcccatgttg ttcatacgct caacatcatt cggttttcgg caacctgcca
    42841 gcacatgaaa accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac
    42901 atccggtaat taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat
    42961 gagtcatgat ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg
    43021 gcgtcgcggt tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct
    43081 tccataaaaa agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg
    43141 ctaaaggctt cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat
    43201 ggttcagcgt tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc
    43261 tgtggctgaa aaccacgcaa accatcattg cgcccagttc aaccagcacc caacgcggct
    43321 gatgctgttt gagcagagcc ggaaggcgcg ccagcccttg ttgcgaggtg tcgccgctga
    43381 tgctggcatt aactaccgac gttttactct gccacttatc attcaacaag gcaggccagg
    43441 ccgcgctggc agacattcga tacccggcgc tcaggctatc acccagaatc aataacgtgt
    43501 ccgctgcggc ggcacggaag gttaacagga ccaggaacag gaagggcaaa tgccagcgga
    43561 aaacattgtt gaagttcatc atcttaagaa gtccgtcggt cagggggagc atgaactctc
    43621 catcctcacc ggagttgagc tggttgtcaa acgtggcgag accatcgcac tggtgggcga
    43681 gtcgggatcg ggtaagtcaa ccttgctggc gatcctcgcc gggcttgatg acggcagcag
    43741 tggcgaagtg agtctggtgg gacaaccgct acataatatg gacgaagaag cgcgggcaaa
    43801 gttgcgcgcg aagcacgtcg gctttgtttt tcagtcattt atgttaattc ctacccttaa
    43861 cgcgctggaa aacgtcgagc ttccggctct gctgcgcggt gagagtagcg cggaaagtcg
    43921 taacggggcg aaagcgttgc tcgaacagtt agggctgggt aaacgtctgg atcatcttcc
    43981 ggcacagctt tccggcggtg aacagcaacg agtggcgctg gcacgagcct ttaatggtcg
    44041 acctgatgtg ctgtttgccg acgaacccac cggcaacctt gaccgccaga cgggcgataa
    44101 aattgccgac ctgctgtttt ccctcaaccg tgaacatggc accacgttga ttatggtgac
    44161 ccacgacctg caactggcgg cacgctgcga ccgctgctta cggctggtga acgggcagtt
    44221 gcaggaggaa gcatgattgc acgttggttc tggcgcgaat ggcgttcgcc gtcgctatta
    44281 attgtctggc tggcgctaag cctggcggtg gcctgcgtgc tggcgctggg caatatcagc
    44341 gatcgcatgg agaagggctt aagccagcaa agccgtgagt ttatggcggg cgatcgggcg
    44401 ttgcgcagtt cacgcgaagt gccgcaagcg tggctggagg aagcgcaaaa gcgcggcctg
    44461 aaagtcggca agcagctgac tttcgccaca atgacctttg caggcgacac accgcagctg
    44521 gcgaacgtca aagcggtgga tgatatctac ccgatgtatg gcggtctgca aactaatccc
    44581 cctggcctga aaccgcaggc gggcagcgta ttgctggccc cacgcctgat ggcactgctt
    44641 aacctgaaaa cgggcgacac cattgacgtg ggcgatgcca ccttgcggat tgccggagaa
    44701 gtgattcagg aaccggattc cggttttaac cccttccaga tggctccgcg tctgatgatg
    44761 aatctggcgg atgtcgataa aaccggagcc gtgcagccgg ggagtcgggt cacctggcgt
    44821 tataaattcg gcggcaacga gaaccagctc gacggctatg agaaatggtt gttacctcag
    44881 cttaaacccg aacaacgctg gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg
    44941 atggaacgct cgcaacagtt cctgctgctt tcggcgcttc tgaccttgct gctggcagtg
    45001 gcagcggtgg cggtagcgat gaatcattac tgtcgcagtc gctacgatct ggtggcgatc
    45061 ctcaaaacgc tgggggcagg gcgagcgcaa ctgcgtaagc taatcgtcgg tcagtggttg
    45121 atggtgctga cgctttcagc cgttaccggt ggggccatag gcctgttgtt cgaaaacgtg
    45181 ttgatggtgc tgctcaagcc cgttctgcct gctgcactac cgccagccag cctctggccg
    45241 tggctgtggg cgcttggcac catgacggtc atctcgctgc tggtggggct acgaccatac
    45301 cgcttgttgc tggcaacgca gcctttacgc gtattacgta atgatgtggt cgcgaacgtc
    45361 tggccgctga agttttatct gccgattgtc agtgtggtgg ttgtgctgct gctcgccgga
    45421 ttaatgggtg gcagcatgct gctttgggcg gtgctggcgg gcgcggtagt actggctttg
    45481 ctgtgcggtg tgctgggctg gatgctgctg aatgtacttc gccgcatgac gctgaaatcg
    45541 ctgcctctgc gcctggcggt tagccgcctg ttacgtcagc cgtggtcaac gttaagccag
    45601 ctttcggcat tttcgctctc ctttatgctg ctggcactgc tgctggtgct gcgtggcgat
    45661 ctgctcgacc gctggcagca gcagctaccg ccagaaagcc cgaactactt tttaattaac
    45721 atcgccacag aacaggtagc accgctaaaa gcgttcctcg cggaacatca tatagtcccg
    45781 gaatcatttt atccggtagt gcgggcgcgg ctgacggcga ttaatgataa gccgacagaa
    45841 ggtaatgaag atgaggcgct taatcgcgaa ctcaatctta cctggcaaaa tacgcggccc
    45901 gatcataatc cgattgtcgc cggtaactgg ccgccaaaag ccgatgaagt gtcgatggaa
    45961 gaggggctgg cgaaacgctt aaacgttgcc ctcggtgata ccgtgacttt tatgggcgat
    46021 acccaggagt tccgcgctaa agtgaccagc ctgcgcaaag tggactggga aagtctgcgg
    46081 cctaatttct attttatttt ccctgaaggg gcattagacg ggcaaccgca gagctggctt
    46141 accagtttcc gctgggagaa tggcaacggc atgttgacgc aactcaaccg ccagttcccg
    46201 accattagcc tgttagacat tggcgcgatt ttaaaacagg tcggtcaggt gctggagcag
    46261 gtaagtcggg cgctggaggt gatggtggtg ctggtcaccg cctgcggtat gttgctgttg
    46321 ctggcacagg tgcaggtggg aatgcgccag cgtcatcagg agctggtggt atggcgcaca
    46381 ctcggtgcgg ggaaaaaact gctgcgcacg acattgtggt gtgatgttcg ccatgcttgg
    46441 gtttgtttcc ggcctggtgg ccgcaattgg tgcggaaacg gcactggcag tgttgcaggc
    46501 gaaagtgttt gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag
    46561 cggtgcgctg ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa
    46621 ggcgctgttc aggcagtttg tggggtgatg acaggtgtta gattgcctga tgcgctacgc
    46681 ttatcaggcc tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata
    46741 aggcgtttac gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa
    46801 ctgtcatgtc ggttttttta aaggccgaag gaggtaaacc ttcggcctta gctacgtttc
    46861 ctcagttatt caataaactg acggatcagt tccagcagcg cgattagcgc cttaagaacg
    46921 atgagaactt tatcgagaaa agttttcatc cgttgctccc ttaagggaga aagacaatca
    46981 cagcttaccc ttacgttgcc tataactatg attttcttca tgtgctagat gaggactgtc
    47041 tctctccaaa ttagagcacc agaatcagca ccacctgtat cctgaccagg acttaaaagt
    47101 acttttccgc actgcggtaa tcccttgagg cagtatcact actagcgtcg ggattgtatc
    47161 gccaacatag ctgtttgaat atgtaggcat cctgacttat aattcacttg ctagatgagg
    47221 actgtttaat ttctgcccgg aaccaccaag tcttgggcgg aaaaaataaa aaaaccgact
    47281 gtcatgtcgg tttttttata tctgtattct ggtgcaacca ttttgttctc acgacaatcg
    47341 ccgtttcaga ataccgccca tcactgcgcc ttaacccata ccgccacttc cgcccactca
    47401 ccgcgaaaga caactttttc cgcttttttc tcaagctgat agcgatacat tgggtcgtaa
    47461 tattctttaa gtaacggcac cagccaggcc agatgaccgt cggtgctgcc ggtggtgagt
    47521 tgcgttgtca gcgccgtatc cagctgggca gcgagttcgt tatagcgctg taaccccagc
    47581 cgacgcttaa tcgccgaaag tccgtgatgc aggtattcgc aatattcctg ccagccctgt
    47641 tcgtcgccat aagcgtgggt aaaatcatga tgcatacgca agaaatactc ttcattcagg
    47701 cgttcaagac ggatctcaaa cggatcttct accaccgcaa tcgccgcctg agtcattcgc
    47761 tcgcgcagac attccggcag gtgattcgaa ccgatcatcc ggctttcatc ttccagcacc
    47821 cacaggcgca aatcctgacg ggcgtcggtt tttagcattt cggcagccag caggttttca
    47881 aaactcgcct ggctaagttg tggttgtaac gtgcgaccaa acgccgaacc gcgatgacgc
    47941 gccagccctt ccagatcaac accgtttggc tgttgttgca ctaatagcgt tttaccgctg
    48001 ccggtacaac cgccaatcag cactatcggt ttttgtgcca gttcaatggt cgcctgaatc
    48061 gcggtctggc gcagtgcctt ataaccgcct tccaccagcg gataatcaat ccccgcttca
    48121 tgcagccagc gttgcacaat atgtgaacgt tgaccgccac gggcgcagca gagaatacct
    48181 tgcggatttt gcaggcacgc tgcccgccag gcgtacatac gctgctgacg aatttcaccc
    48241 gccaccagtt tatgccccag cgccagcgct gcgtctgagc cttgctgttt atagcaggtg
    48301 ccaacggagg cgcgttcatc gttatccatt aacggcagat tgatagcggc gggcattgcg
    48361 ccttgctcaa actcgatagg ggcgcgaaca tcaataatgg gcgtatcagc aatcagcagg
    48421 gcacgatagt cctgttccgt gtgtctctct tgcatagtta aaagtgaacc tcaaatcagc
    48481 ttgcgcgcta ttttacgcgc caacgcgcaa ggaaacttga tttttaactg cgtggggtgc
    48541 cgaaaatttc taaaaatccg ctgatttccg gcctgtgttg ggtaaacaag gtcacaatat
    48601 cttctaccgc tttgcagctg ccaaatttgc gccatgccag actcaatggc gaaggagggc
    48661 gcatcgttgg gattacccgg ctgaccagtt gttgattatc gatcattgac tggcaaagtg
    48721 attttggcaa aaaaccaatg ccaacacccg ccagatgggc ggcgattttc gtttccatat
    48781 cgggaacaat aatctctttt tgccccggca atcgccaggc gacgcgtttg gttaaggtgc
    48841 cggcgctgtc ttcaatattg accgccggaa agcgacgcaa ctgcgcttct gttagcggct
    48901 cttcaacgtt cgccagcgga tgatccgccg ccatgacaaa tcgccattgc accgatccta
    48961 agggatcaag actaaaggta tttgccagcg cctcagttcc cgtgaccccg atagccagcg
    49021 aaaaaccttc gtacaatagc gagtcccaga cgcccatata gatttgtcgg gagatgtgaa
    49081 actgggtaaa ggggtaacgc tcattcagcc acgccagcaa ctgggcgacg gcctgggggt
    49141 tatagagcag gttgttgatg acaatattca cctggcgttc cacgccatca ttcacctgtt
    49201 gcagctcgct gggcatactt tccagccagc tcagccagtc tctggcctgg gaaagtagat
    49261 gctcgccagc cgctgtcaac gttacgctac gagtcgtacg gaaaaacagc gctactccgg
    49321 tattctcttc cagaagttta atgcgataac tgatcgtcgc cgtggtttta cataatcgtt
    49381 ctgccgcttt tgaaaaactt cctgtttcag caaccgcaat gaaagtccgc aaggtttctg
    49441 gatcgaacat cttcaggtat ccccttttaa atccgcaagt tgcgtgattt tcttatcctc
    49501 tgatttatca gtatttttac atgataatcc tgttcaattt gtggactaaa tctagttttg
    49561 gaaaaacatt ccaacttttg tattaatgtt gttctcttaa ggttttagat tgcctgttat
    49621 tgaaaccaag ctgaccggtc ggcggtggtt gaacggaatt atgttacaag gacaaaaaga
    49681 tgaaacttca ggtattaccg ttaagtcagg aagcctttag tgcttatggc gacgtaatcg
    49741 aaacgcagca acgggatttt ttccatatta acaatggcct ggtggagcgt taccacgatt
    49801 tggcgctggt tgagattctt gagcaagacc gtacgcttat cagcattaac cgcgcgcaac
    49861 cggcgaatct gccgctgacc attcacgaac tcgaacgtca tccgctgggc actcaggcct
    49921 ttatcccaat gaaaggtgaa gtgtttgtgg tggtcgtggc gttaggcgac gacaaaccag
    49981 acctgtcaac gctgcgggcg tttatcacca acggtgaaca gggggtgaat taccatcgta
    50041 acgtctggca tcacccactt ttcgcctggc agcgcgtcac cgattttctg accatcgatc
    50101 gcggcggcag tgacaactgt gatgttgaaa gtattcctga acaggaactc tgttttgcgt
    50161 gacgcctgca accgacttgc ataagataaa ctaattgttc attgtttatg ctcacttgta
    50221 ggtcggagtt aacgtaggta tgacggaagt tagacggcgc ggcaggccag gacaggcgga
    50281 gcctgtggca cagaagggcg cacaggcgtt agagcgggga attgcgattc tgcaatatct
    50341 ggaaaaaagt gggggaagtt cgtcggttag cgatatttct ctcaatctgg atttgccgct
    50401 ctccacgacc tttcgcttgc tgaaggtttt acaagcagcg gattttgtct atcaggacag
    50461 tcaattaggc tggtggcata taggattagg tgtctttaac gtcggtgcgg cgtacatcca
    50521 taaccgcgat gtcctctccg tcgccgggcc gtttatgcgc cgcctgatgt tactttccgg
    50581 cgaaacggtc aatgtcgcga tccgtaacgg caatgaagcg gtattaattg gtcagttaga
    50641 gtgtaaatcg atggtcagga tgtgtgcgcc actgggcagt cgtctgccgc tgcatgcttc
    50701 cggtgcgggc aaagcgctgc tttatccgct ggcggaagag gagttgatga gcatcattct
    50761 gcaaaccggt ttgcagcagt ttacgccaac tacgcttgtg gatatgccca ccttgctgaa
    50821 ggacctggaa caagcgcgtg aactgggcta taccgtagat aaagaagagc atgttgtagg
    50881 tctgaattgc atagcttcag caatttacga tgatgtcggt agagttgttg ccgctatctc
    50941 catctccggg ccttcatcaa gactgacaga agatcgtttt gtcagtcagg gtgagctggt
    51001 cagagacacc gcccgcgata tcagcacggc gttgggactg aaagcacatc catagtgtct
    51061 gtcgcatccc gctctgcgga gcgggttttt ttgacaaaat ttgaaagttg gaaaattttt
    51121 ccaactaata gaggtaggaa taaaatggca aaaatgagag ccgttgacgc ggcaatgtat
    51181 gtgctggaga aagaaggtat caccaccgcc ttcggcgttc cgggagctgc aattaatccg
    51241 ttctactcag cgatgcgtaa gcacggcggt attcgtcaca ttctggcgcg tcatgtggaa
    51301 ggtgtttcgc acatggcgga aggttatacc cgcgcaacgg cagggaatat cggcgtatgt
    51361 ctggggactt ccggtcctgc gggcacggac atgatcaccg cgctctattc cgcttctgct
    51421 gattccattc ctattctgtg cattaccggc caggcaccgc gcgcccgtct gcataaagaa
    51481 gattttcagg ccgtagatat tgaagcaatt gctaaaccgg tcagcaaaat ggcggttaca
    51541 gttcgtgaag cggcgctggt gcctcgcgtg ctgcaacagg catttcacct gatgcgttct
    51601 ggtcgtccgg gtccggtact ggtggattta ccgttcgacg ttcaggttgc ggaaatcgag
    51661 tttgatcctg acatgtacga accgctgccg gtctacaaac ctgctgccag ccgtatgcag
    51721 atcgaaaaag ctgtagaaat gttaatccag gccgaacgtc cggtgattgt tgccgggggc
    51781 ggggtaatca atgctgacgc agctgcactg ttacaacagt ttgctgaact gaccagcgtt
    51841 ccggtgatcc caacgctgat gggctggggc tgtatcccgg acgatcatga actgatggcc
    51901 gggatggtcg gtctgcaaac cgcgcatcgt tacggtaacg caacgttgct ggcgtccgac
    51961 atggtgtttg gtatcggtaa ccgttttgct aaccgtcata ccggttcggt agagaaatac
    52021 accgaagggc gcaaaatcgt tcatattgat attgagccga cgcaaattgg tcgcgtgctg
    52081 tgtccggatc tcggcattgt ctctgatgct aaagcggcgc tgacactgct ggttgaagtg
    52141 gcgcaggaga tgcaaaaagc gggtcgtctg ccgtgtcgta aagaatgggt cgccgactgc
    52201 cagcagcgta aacgcacttt gctgcgcaaa acccacttcg acaacgtgcc ggtgaaaccg
    52261 cagcgcgtgt atgaagagat gaacaaagcc tttggtcgcg atgtttgtta tgtcaccact
    52321 attggtctgt cacaaatcgc tgcggcacaa atgctgcatg tctttaaaga ccgccactgg
    52381 atcaactgtg gtcaggctgg tccgttaggc tggacgattc cggcggcgct ggggggttgt
    52441 gccgctgatc cgaaacgcaa tgtggtggcg atttctggcg actttgactt ccagttcctg
    52501 attgaagagt tagccgttgg cgcgcagttc aacattccgt acatccatgt gctggtgaat
    52561 aacgcctatc tcggcctgat tcgtcagtca caacgcgctt ttgacatgga ctactgcgtg
    52621 caactcgctt tcgagaatat caactccagt gaagtgaatg gctacggtgt tgaccacgta
    52681 aaagtagcgg aaggtttagg ttgtaaagct attcgggtct tcaaaccgga agatattgcg
    52741 ccagcctttg aacaggcgaa agccttaatg gcgcaatatc gggtaccggt agtcgtggaa
    52801 gttattctcg agcgtgtgac caatatttcg atgggcagcg aactggataa cgtcatggaa
    52861 tttgaagata tcgccgataa cgcagcggat gcaccgactg aaacctgctt catgcactat
    52921 gaataaggga gataaatgat gttacgtttc tctgctaatt tatcgatgtt atttggtgaa
    52981 tatgattttc tcgcccgttt tgagaaagct gcgcagtgtg gttttcgcgg cgttgaattt
    53041 atgtttcctt atgactacga cattgaagaa ttaaaacagg tactggcgag taataaactc
    53101 gaacatacgc tgcacaattt accggcaggt gactgggcgg cgggcgagcg cggtattgcc
    53161 tgtattcctg gccgtgaaga agagtttcgg gatggcgtag cagcagcgat t
//
LOCUS       DAJTSA010000018        52599 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000018 DAJTSA010000000
VERSION     DAJTSA010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.018"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..356
                     /locus_tag="OO369_001126"
     CDS             <1..356
                     /locus_tag="OO369_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR5981478.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
     gene            complement(394..1293)
                     /gene="nhaR"
                     /locus_tag="OO369_001127"
     CDS             complement(394..1293)
                     /gene="nhaR"
                     /locus_tag="OO369_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR5981479.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1359..2525)
                     /gene="nhaA"
                     /locus_tag="OO369_001128"
     CDS             complement(1359..2525)
                     /gene="nhaA"
                     /locus_tag="OO369_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR5981480.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3111..3263
                     /gene="mokC"
                     /locus_tag="OO369_001129"
     CDS             3111..3263
                     /gene="mokC"
                     /locus_tag="OO369_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR5981481.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3367..4497)
                     /gene="dnaJ"
                     /locus_tag="OO369_001130"
     CDS             complement(3367..4497)
                     /gene="dnaJ"
                     /locus_tag="OO369_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR5981482.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4586..6502)
                     /gene="dnaK"
                     /locus_tag="OO369_001131"
     CDS             complement(4586..6502)
                     /gene="dnaK"
                     /locus_tag="OO369_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR5981483.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6879..7283
                     /locus_tag="OO369_001132"
     CDS             6879..7283
                     /locus_tag="OO369_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR5981484.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7309..8022
                     /gene="msyB"
                     /locus_tag="OO369_001133"
     CDS             7309..8022
                     /gene="msyB"
                     /locus_tag="OO369_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR5981485.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8171..8737
                     /gene="satP"
                     /locus_tag="OO369_001134"
     CDS             8171..8737
                     /gene="satP"
                     /locus_tag="OO369_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR5981486.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8772..9359)
                     /gene="mog"
                     /locus_tag="OO369_001135"
     CDS             complement(8772..9359)
                     /gene="mog"
                     /locus_tag="OO369_001135"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR5981487.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9474..10427)
                     /gene="tal"
                     /locus_tag="OO369_001136"
     CDS             complement(9474..10427)
                     /gene="tal"
                     /locus_tag="OO369_001136"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5981488.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10706..12136
                     /locus_tag="OO369_001137"
                     /pseudo
     CDS             10706..12136
                     /locus_tag="OO369_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            12206..12982
                     /gene="yaaA"
                     /locus_tag="OO369_001138"
     CDS             12206..12982
                     /gene="yaaA"
                     /locus_tag="OO369_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR5981489.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLVSDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13135..13431)
                     /locus_tag="OO369_001139"
     CDS             complement(13135..13431)
                     /locus_tag="OO369_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5981490.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13645..14931)
                     /gene="thrC"
                     /locus_tag="OO369_001140"
     CDS             complement(13645..14931)
                     /gene="thrC"
                     /locus_tag="OO369_001140"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR5981491.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14932..15864)
                     /gene="thrB"
                     /locus_tag="OO369_001141"
     CDS             complement(14932..15864)
                     /gene="thrB"
                     /locus_tag="OO369_001141"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR5981492.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15866..18328)
                     /gene="thrA"
                     /locus_tag="OO369_001142"
     CDS             complement(15866..18328)
                     /gene="thrA"
                     /locus_tag="OO369_001142"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR5981493.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18354..18473)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18409..18474)
                     /gene="thrL"
                     /locus_tag="OO369_001143"
     CDS             complement(18409..18474)
                     /gene="thrL"
                     /locus_tag="OO369_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR5981494.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18688..19374)
                     /locus_tag="OO369_001144"
     CDS             complement(18688..19374)
                     /locus_tag="OO369_001144"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5981495.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19774..19914)
                     /locus_tag="OO369_001145"
     CDS             complement(19774..19914)
                     /locus_tag="OO369_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981496.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20010..20726
                     /gene="arcA"
                     /locus_tag="OO369_001146"
     CDS             20010..20726
                     /gene="arcA"
                     /locus_tag="OO369_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR5981497.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20785..22137)
                     /gene="creD"
                     /locus_tag="OO369_001147"
     CDS             complement(20785..22137)
                     /gene="creD"
                     /locus_tag="OO369_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR5981498.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22195..23619)
                     /gene="creC"
                     /locus_tag="OO369_001148"
     CDS             complement(22195..23619)
                     /gene="creC"
                     /locus_tag="OO369_001148"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR5981499.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23619..24308)
                     /gene="creB"
                     /locus_tag="OO369_001149"
     CDS             complement(23619..24308)
                     /gene="creB"
                     /locus_tag="OO369_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR5981500.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24321..24794)
                     /gene="creA"
                     /locus_tag="OO369_001150"
     CDS             complement(24321..24794)
                     /gene="creA"
                     /locus_tag="OO369_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR5981501.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25005..25874
                     /gene="robA"
                     /locus_tag="OO369_001151"
     CDS             25005..25874
                     /gene="robA"
                     /locus_tag="OO369_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR5981502.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25871..26518)
                     /gene="gpmB"
                     /locus_tag="OO369_001152"
     CDS             complement(25871..26518)
                     /gene="gpmB"
                     /locus_tag="OO369_001152"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR5981503.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26570..27090
                     /gene="yjjX"
                     /locus_tag="OO369_001153"
                     /pseudo
     CDS             26570..27090
                     /gene="yjjX"
                     /locus_tag="OO369_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(27175..27501)
                     /gene="trpR"
                     /locus_tag="OO369_001154"
     CDS             complement(27175..27501)
                     /gene="trpR"
                     /locus_tag="OO369_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR5981504.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27591..29528)
                     /gene="sltY"
                     /locus_tag="OO369_001155"
     CDS             complement(27591..29528)
                     /gene="sltY"
                     /locus_tag="OO369_001155"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR5981505.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29739..31406
                     /gene="ettA"
                     /locus_tag="OO369_001156"
     CDS             29739..31406
                     /gene="ettA"
                     /locus_tag="OO369_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR5981506.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31513..31680)
                     /locus_tag="OO369_001157"
     CDS             complement(31513..31680)
                     /locus_tag="OO369_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981507.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31714..32946)
                     /gene="nadR"
                     /locus_tag="OO369_001158"
     CDS             complement(31714..32946)
                     /gene="nadR"
                     /locus_tag="OO369_001158"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR5981508.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEILEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32967..34349)
                     /gene="radA"
                     /locus_tag="OO369_001159"
     CDS             complement(32967..34349)
                     /gene="radA"
                     /locus_tag="OO369_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR5981509.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34398..35366)
                     /gene="serB"
                     /locus_tag="OO369_001160"
     CDS             complement(34398..35366)
                     /gene="serB"
                     /locus_tag="OO369_001160"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR5981510.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35472..36116
                     /locus_tag="OO369_001161"
     CDS             35472..36116
                     /locus_tag="OO369_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR5981511.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36144..37159
                     /gene="lplA"
                     /locus_tag="OO369_001162"
                     /pseudo
     CDS             36144..37159
                     /gene="lplA"
                     /locus_tag="OO369_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37191..37454
                     /locus_tag="OO369_001163"
     CDS             37191..37454
                     /locus_tag="OO369_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5981512.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37615..38334)
                     /gene="deoD"
                     /locus_tag="OO369_001164"
     CDS             complement(37615..38334)
                     /gene="deoD"
                     /locus_tag="OO369_001164"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR5981513.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38391..39614)
                     /gene="deoB"
                     /locus_tag="OO369_001165"
     CDS             complement(38391..39614)
                     /gene="deoB"
                     /locus_tag="OO369_001165"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5981514.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39666..40988)
                     /gene="deoA"
                     /locus_tag="OO369_001166"
     CDS             complement(39666..40988)
                     /gene="deoA"
                     /locus_tag="OO369_001166"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR5981515.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41115..41894)
                     /gene="deoC"
                     /locus_tag="OO369_001167"
     CDS             complement(41115..41894)
                     /gene="deoC"
                     /locus_tag="OO369_001167"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR5981516.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42152..43693
                     /locus_tag="OO369_001168"
     CDS             42152..43693
                     /locus_tag="OO369_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR5981517.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43665..44528
                     /locus_tag="OO369_001169"
     CDS             43665..44528
                     /locus_tag="OO369_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR5981518.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44742..45524)
                     /locus_tag="OO369_001170"
     CDS             complement(44742..45524)
                     /locus_tag="OO369_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5981519.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45521..46594)
                     /locus_tag="OO369_001171"
     CDS             complement(45521..46594)
                     /locus_tag="OO369_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR5981520.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46716..46877)
                     /locus_tag="OO369_001172"
     CDS             complement(46716..46877)
                     /locus_tag="OO369_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR5981521.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(46994..47599)
                     /gene="osmY"
                     /locus_tag="OO369_001173"
     CDS             complement(46994..47599)
                     /gene="osmY"
                     /locus_tag="OO369_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR5981522.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(47992..49581)
                     /gene="prfC"
                     /locus_tag="OO369_001174"
     CDS             complement(47992..49581)
                     /gene="prfC"
                     /locus_tag="OO369_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR5981523.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49672..50349)
                     /gene="yjjG"
                     /locus_tag="OO369_001175"
     CDS             complement(49672..50349)
                     /gene="yjjG"
                     /locus_tag="OO369_001175"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR5981524.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50364..50810)
                     /gene="rimI"
                     /locus_tag="OO369_001176"
     CDS             complement(50364..50810)
                     /gene="rimI"
                     /locus_tag="OO369_001176"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5981525.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50779..51192)
                     /gene="holD"
                     /locus_tag="OO369_001177"
     CDS             complement(50779..51192)
                     /gene="holD"
                     /locus_tag="OO369_001177"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR5981526.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51295..52326
                     /gene="rsmC"
                     /locus_tag="OO369_001178"
     CDS             51295..52326
                     /gene="rsmC"
                     /locus_tag="OO369_001178"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR5981527.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        12527 a        14234 c        13180 g        12658 t
ORIGIN      
        1 attatcagtt tcatcatata agatttgact gcatccaggg cgagcaaggt gaattagttt
       61 ttgaacccag tgacgttgaa tattactttg aaccagtgac gatacaggag tcaggaacca
      121 gcatattaaa aaatgagtta gaaaacagtg aggacggtgc gggtcaaata ggctttcaac
      181 tctccaatga tggtacgaat gaaatccagt atggtaaaag caattactac tcttttcagc
      241 atccccatga aggaagtaat cagattcctc tatttattcg cccgcgtaca tatggaaaca
      301 atgtctcaag cgggcaaata atgagccgag tcaaaatcgt ggtaatgtac aactaatcta
      361 tctcccccac cttaactttg ggggagctgc cgattaacgc accgctggac taaaaagcgc
      421 agaataatcc gtattgcaga ttcgctgtac cgccgggtgc tgaatcatcc gctcagcaaa
      481 aatagcatgg tactcttcca tcacattctc gacgcgacca atttctacga cagttttatc
      541 ggcataaaag tcatatgcat aaagcgttgg ggcaacgaag attgcattgt gcatcgcacc
      601 aaaagctttc atcaaagcgg catcatcaaa ctcgccgagg atttctacgt ttaatccctg
      661 ggagttaaac cagttaagca atttgcgccc taacattgaa cgtcgcccag gaatcaaaag
      721 tcgccgttct tccagacaag ccgggaacgg tttttctggt ggtggatttg tacaccagaa
      781 actcacgcca cattcgccaa ttctcacgga gaacaggcct tcctgctgcg tagagtctat
      841 cggacagtca gaaatgatca tatccagttt atgctgactt aattgctcca gcagcatttc
      901 gtgggtggat tcgaagcagc gaagatgaat gggctcgcct tctaccactg cggcgttaag
      961 tacgctactg accaggcgtt tggaaagtgc atcagccacg ccaacgtcaa acaataaatt
     1021 ggattctttg cgatagttca caatatccag catttcctgg cttaaggtga acattttatc
     1081 ggcatagcga tagaccagtt ctcccagctc gctgggttcg agaccacgtc ccttgcgttt
     1141 aaataatttg ccttgcaggc gctcttccag cgctcgaatc tgtccggtaa tggtttgtgg
     1201 agttaaataa agcgcctccg ctgcgccaac cacggaacct tctttataga catgccagaa
     1261 gtaatacaag tggttgtaat tgatatgaga catgctcatt tctctccctg ataacaatga
     1321 aaagggagcc gtttatggct ccccggtaaa ccgtcctgtc aaactgatgg acgcaaacga
     1381 acgcgtaacc agctgtatcc aattaccgcc gaagagatag aaccgaccag gataccgagt
     1441 ttcgcccagt taatcagttc tggatctacg ctaccaaagg ccaggctggc aataaagata
     1501 gacatagtaa aaccgatacc gcacaggatc cccaccacca taatttgctg ataagtcgtt
     1561 ccctcaggca gatgcgccag tttcaaacgc agcgccaacc agcagaacag actaatcccc
     1621 agcggtttgc caatcagcaa gccagcgatg atccccaatg gcagaatgga ggtcaagcca
     1681 tccagcgtga cgccttgcag tgaaacgcca gcattagcaa atgcaaacag cggcaaaatc
     1741 agatacgcca cccacgggtg caacacatgc tccagtcgct tcgctggaga acgcccatgc
     1801 ttctctttca aaggaataaa gaagccgaca attacccccg ccagagttgc gtgaaccccc
     1861 gatttcaaca ccgcagtcca caacaccacg ccaacaagaa tatagacgcc cgtgcggcgt
     1921 acaccacaca gattcaatac cgcgagtacc gcaattgcta cagccgcgac gccaagggag
     1981 gccatcgata agtcattagt gtagaacaat gcgatgataa tgatggcccc aagatcgtcg
     2041 ataatagcca gagccatcaa aaagatcttc agcactaacg gaacacgact tcccaacagc
     2101 gccagtacac caagtgcaaa agcaatgtca gtagccgccg ggatcgccca cccttcgcgg
     2161 gtaatcggat cggcatagtt aaaagccaga tagagtaatg ccggcacaat catcccacca
     2221 atagcggcga taactggaaa tgcggcctgg cgtaagctgg ctagcgatcc ttgcatcagt
     2281 tcacgtttaa cttccagacc gactaacagg aaaaataccg ccatcagcgc atcatttatc
     2341 cataacagca tgtttttgtt gatttcgagt gaaccaaccc ggagctgaac cggcgtctcc
     2401 agaaagtcgt gataccatcc actggttgcg ccgctgttgg ccataatcat cgccaggata
     2461 gcggcaatga taagaataat gcctcccgag gcatcactgc taaagaatcg atgcagatgt
     2521 ttcacttttt atttctcttt caggtgaata gatcgttttt actattttac cccgcacgaa
     2581 tcatcgttaa aatcagatta tagtgtagga ataattcgtt ttttacgagc cattgaagaa
     2641 tttgcgccat cagcggtgag atgcgttttt gacggctgta agcgctcacg agtgacccta
     2701 agcccagata atcatagctt aaggtgagga ttcctgaggg attaattagg aaaagtaata
     2761 attaatttgt tgtcagtcgg cgatgcttgt taggccaata tttcaaaaat aatccagctt
     2821 gtcgtcaata attgcgatga agagataaat taaagttaaa taattaaatc attaaaaaat
     2881 tacgccacaa aaatgcgagg cgtcttcagg attagaatcg ataaaacaaa atgggttaaa
     2941 gagagcgata taatagcggc gggtgcttga ggctgtctgt ctcaggcatt agctgaacgg
     3001 cagatagaga aaagccccga gtgatatttt accatcaacc cgaggcctcc tatatgctga
     3061 acacatgtag agtgcctctt actgaccgta aggtcaagga gaagagagca atgaagcagc
     3121 ataaggcgat gattgtcgcc ctgatcgtca tctgtatcac cgccgtagtg gcggcgctgg
     3181 taacgagaaa agacctctgt gaggttcaca tccgaactgg ccagacggag gttgctgttt
     3241 tcacggctta cgaatccgag taagagcaac ggcggggagt gatccccgcc actttacagg
     3301 tgctcgcata tcttcaacgc accctatttt tacccaggcc tgcccacggg caggcttttg
     3361 gggaggttag cgggtcaggt cgtcaaaaaa cttcttcaca ccatcaaaga agctctttga
     3421 gcgcgggctg ttgtgctcgc cggttgggcc accgaagctt tcttgcagct cttgcagcag
     3481 ctgtttctgc ttttcgttca ggcctaccgg tgtttcgacg acaacgcggc acagcaaatc
     3541 accctgtgcg ccaccgcgga cagacttgac gcctttaccg cgcatacgga atagcttacc
     3601 ggtctgggtt tcgccaggca ctttcagttt gacgcgacca tcaagggtcg gtacttcgat
     3661 ttcgccaccc agcgccgcca tagcgaagtt gatcgggact tcgcaataca ggttgttgcc
     3721 ttcacgctcg aaaatcgggt gctgtttaac ctgaacctga acgtacagat cgcctgccgg
     3781 tgcgccatgc tcgcccgctt caccttcgcc cgcaagacgg atgcggtctc cagtgtccac
     3841 ccctgccggg attttaacgg acagcgtttt gctgcgctca acacgaccat gaccatgaca
     3901 tttgttgcac ggatctttga tcagcgtacc gcggccctga cagtgtggac aggtctgctg
     3961 tacagcgaag aatccctggc gcatctgcac ctgaccagaa ccatgacagg tcggacaggt
     4021 ctgcggctgt gtacctggtt ttgcaccgct accgtggcaa acgtcacact cttccagagt
     4081 cggaatgcgg atctctttgg tcacgccacg tacagcttct tcgagggtga gctccatgtt
     4141 atagcgtaaa tcagcaccgc gcgccgcacg ttgacgacca cgtccgccgc caaaaatatc
     4201 gccgaaaacg tcaccaaaaa tatcgctgaa gtctgcgccg ccgccaaaac caccgccgcc
     4261 catgcctcct tgctcaaacg cagcatgacc atactgatcg tatgccgcac gtttttgcga
     4321 gtcggtcaga acttcataag cttccttgat ctctttaaat ttcgcctcgg cctctttgtc
     4381 accctggtta cggtccgggt ggtatttcat ggccaggcgt ttgtaggcct ttctgatttc
     4441 acgctcttcc gctgttttgg aaacgcctaa aatctcgtaa taatcttgct tagccatctt
     4501 ttttaaattg cccctagatg aatgcacggg cggagaggaa attccccttc gcccgtgtca
     4561 gtataattac ccgtttatag ggcgattatt ttttgtcttt gacttcttca aattcagcgt
     4621 cgacaacatc gtcatctttc gcgttgtttg cagaagcatc agcaccggca gtctgctgct
     4681 gggcatgttg ctgctgggcg atttccatca gtttctggga aacctgtgcc agttcctgca
     4741 ttttcgcttc gatagcggct ttgtcttcac ctttcagagc agtttccagt gcagtcagcg
     4801 cagactcgat agcagttttg tcgtcagccg gcagtttgtc gcctgcttct tcaacctgct
     4861 tacgggtgct gtgcagcaga tggtcgccct ggttgcgagt ctgtaccagc tcttcaaact
     4921 tacggtcagc ttcggcgtta gcttctgcgt cgcgtaccat tttctggatt tcatcttcgt
     4981 tcagaccaga agaagccttg atggtgatct tctgctcttt accgctgttt ttatctttcg
     5041 cggaaacgtg caggataccg tcagcatcga tatcgaaggt aacttcgatc tgcggcatgc
     5101 cgcgcggtgc cgggttgata ccatctaggt tgaactgacc cagagatttg ttatcagccg
     5161 cacgtttacg ttcaccctgc agcacatgga tggttaccgc agactggttg tcttcagcgg
     5221 tagagaacac ctggctgtgc ttggtcggga tagtggtgtt tttcgcgatc agcgtcgtca
     5281 tcacaccgcc catggtttcg atacccagag acagcggggt aacgtccagc agcagtacgt
     5341 cttttacgtc accagtcaga acaccaccct gaacagcagc accgattgct acagcttcgt
     5401 ccgggttaac gtctttacgc ggctctttac caaagaactc agcaactttc ttctgaacca
     5461 ttggcatacg agtctgacca ccaacgagga taacgtcgtc gatatcagat acggacaggc
     5521 cagcatcctg cagtgcaact ttcagcggct caatggaacg gtttaccaga tcttcaacca
     5581 ggctttccag tttcgcacga gtcactttga tgttcatgtg tttcggaccg gtcgcgtctg
     5641 cagtgatgta tggcaggtta acgtcggtct gctgagcgga agacagttcg attttcgctt
     5701 tttctgccgc ttctttcagg cgctgcattg ccagcggatc gttgcgcagg tcaatgccct
     5761 gatctttctt gaattcttca accagatagt tgatcagacg gctgtcgaag tcttcaccac
     5821 ccaggtgggt atcaccgttg gttgccagaa cttcgaaggt tttttcgccg tcaacttcgt
     5881 cgatttcgat aatagaaata tcgaaagtac caccccccag gtcataaacc gcgatagtac
     5941 ggttgccagt gcctttgtcc agaccgtaag ccagcgcagc tgcggtcggt tcgttgatga
     6001 tacgttttac ttccagacca gcgatacggc ctgcgtcttt ggttgcctga cgctgagcat
     6061 cgttaaagta tgccggtacg gtgataacag cttcagttac cggttcaccc aggtaatctt
     6121 cagcggtttt cttcattttt ttcagcactt cagcagaaat ctgcggcggt gccattttct
     6181 ggcctttaac ttcgacccat gcgtcgccgt tatcagcagc aataattttg aacggcatga
     6241 tggaaacatc acgctgtact tcttcgtcct ggaagcggcg gccaatcagg cgtttaatcg
     6301 caaacagggt gttttgcggg ttcgtcactg cctgacgttt agccggctga ccaaccagag
     6361 tttcaccatc ctgggtatag gcaatgatag aaggcgtggt gcgatcgcct tcggcgttct
     6421 ccagcacgcg aggaatggtg ccatccataa tcgctacaca agagttggta gtacccaggt
     6481 cgataccaat tattttaccc atctaaacgt ctccactata tattcggtca tcatgtggtt
     6541 gtgagtctgt aataggggcg aaacgtctgg tttcaactgc ccaatttcat ttttttgcag
     6601 actcactcac tgcggttgac tactaaatgg ggtcgtaacc cacgtcatca agggggagat
     6661 gcaaaaattt tttgtgcttt tgcgcagaaa tttcgcgata tggcaggaca agacgtaaaa
     6721 ttgacagcaa ttttattcat ataaagaatg aatcgcagcc aaggcgttca tcaaaaaatt
     6781 gtaataaaaa gaaaagatta cgtgcctgaa tcttctcttt atcagcagta aactagtggg
     6841 tattcatccc cctacctctt cccactaaga gaatccttat gaaatccgtt tttacgattt
     6901 ccgccagcct ggcgattagc ctgatgctgt gctgcacggc gcaggcaaac gaccataaac
     6961 tcctcggcgt cattgcaatg ccgcgtaacg aaaccaacga tctggcgctg aaacttcctg
     7021 tttgtcgcat tgtgaaacgc atacaactct ctgccgacca tggcgattta cagttaagtg
     7081 gtgcatcggt ttatttcaaa gccgcccgta gcgccagtca gagcctgaat attccttcag
     7141 aaataaaaga agggcaaacc actgactgga tcaacattaa cagcgataac gacaataaac
     7201 gctgcgtctc aaaaatcacc ttttccggtc atacggtgaa ctcatcggat atggccacgc
     7261 tgaaaattat cggcgacgac taacgccaga atatagaagc cacaaaaaat gaatgttaat
     7321 tacctgaatg attcagatct ggattttctt cagcattgta gtgaggaaca gttggcaaat
     7381 ttcgcccgat tgctcaccca taatgaaaaa ggcaaaactc gcctctccag catactgatg
     7441 cgcaacgaac tgtttaaatc gatggaaggg catcccgagc aacatcgccg caactggcag
     7501 ctgattgccg gagaattaca gcattttggt ggcgatagta tcgccaacaa actgcgcgga
     7561 cacggtaaat tgtatcgggc cattttgctc gatgtttcaa agcgattgaa gctgaaagcc
     7621 gacaaagaga tgtctacgtt tgaaattgag caacagttac tggaacaatt tctgcgtaat
     7681 acctggaaga aaatggacga ggaacataag caggagtttc tgcacgcggt cgatgccagg
     7741 gtgaatgagc tggaagagct gctgccgctg ctgatgaaag acaaattatt ggcaaaaggt
     7801 gtgtcgcatt tgctttccag ccaactgacc cgcattttac gcacccacgc agcaatgagc
     7861 gtacttgggc atggtttgct gcgcggcgcg gggctgggag gccctgtggg cgcagcatta
     7921 aatggggtta aagcggtcag cggcagcacc tatcgcgtga cgattccagc cgtactgcaa
     7981 atcgcctgcc tgcgccggat ggttagcgcc actcaggtct gataccggaa accggggaaa
     8041 attttcattt tcccctcaaa agatcgtaga cactgcccca ctggctgatt attatgccgc
     8101 gccctgaaaa cactacagtt attcagggaa attatttcac cattcattcg atgatgattt
     8161 ttgaggaatt atgggcaaca ctaagttggc taatccggca ccgctgggcc tgatgggctt
     8221 cggcatgacc accattctgc ttaacctgca caacgtgggt tatttcgctc tggacggtat
     8281 tattcttgcc atgggcattt tctacggcgg catcgcgcaa atttttgctg gtctgctgga
     8341 gtacaaaaaa ggcaacactt tcggtttaac cgcattcacc tcttacggtt ctttctggct
     8401 gacgctggtt gcgattctgc tgatgccgaa actgggtctg accgatgcgc caaatgcaca
     8461 gttccttggt gtctacctgg gtctgtgggg cgtatttacg ctgtttatgt tcttcggcac
     8521 tctgaaaggc gcgcgcgtcc tgcaattcgt tttctttagc ctgaccgtgc tgtttgccct
     8581 gctggcgatc ggtaacattg ccggtaatgc cgcgatcatc cactttgccg gctggattgg
     8641 tctgatctgc ggtgccagcg caatctatct ggcgatgggt gaagtgctga acgagcagtt
     8701 tggtcgcacc gttctgccga ttggtgaatc ccactaattg tcttgagatc cccccgctcg
     8761 gggggatttt tttattcgct aacgtcgcgt cttgcactct tcggtctgaa tgcagcaacc
     8821 acttctggtg ccgtttcaac gtatggccct tccagcaact gaatgcagta cggtacgctg
     8881 gcaaaaatac catgcaccac aacgttaccc tcagcgtcct tcacaccttc cagcgtctct
     8941 ttaatagact tcggctgacc gggtaagtta aggatcagcg cctgtttgcg aatcacgccc
     9001 acctgacgcg aaaggatcgc agttggtaca aaatgcaggc tgatctggcg catctgttca
     9061 ccaaagccag gcatctcgcg gtccgctact gccagcgtcg catcgggcgt tacgtcacga
     9121 cgcgccgggc cagttccgcc cgtggtgagc accagatggc aactcatttc atccaccagc
     9181 tcacacaacg tttgctcgat gatcgcctgc tcatcgggga ttaagcgggt ttccagttca
     9241 aacggcgtgg ttagcgccga tgtcagccat tcttccagcg ctggaatgcc tttatcctga
     9301 taaacgccgc tggatgcgcg atcagagatg gaaactaagc caatacgtaa agtattcatc
     9361 aagatttttc cgtttaaaca atctcgttaa acagaatgat acactgcgaa gggagtgaca
     9421 gacaggcttc agaagaggta gcgtgaccga cttcccggtc acgctaagaa tgattacagc
     9481 agatcgccga tcattttttc cagtttttcc tggtcaatag caaacttacg gataccttcc
     9541 gccagtttat ctactgccat tggatcctgg ttgtgctgcc acaggaactc ggactcagtg
     9601 atacgcgccg gacgcgcttt cacttcgccg gtgtaagaca gtttacgttc gatagcccct
     9661 tcgctctccg ccagctcttt cagcagtgcc ggtgcgatgg tcagacggtc acagcctgcc
     9721 agttccagaa tttcgccgat gttacggaag cttgcgccca taaccacggt ttcataaccg
     9781 tgctctttgt agtactggta gatttcagat acagaaacca cgcccggatc ttctgccgga
     9841 gcgtactctt tcttatcggt attcgctttg taccagtcaa gaatacggcc aacaaacggc
     9901 gagatcagga acacgcccgc ctccgcacaa gcacgagcct gagcgaagga gaacagcagg
     9961 gtcaggttac agttgatacc ttctttttcc agctgttctg cagcacggat accctgccag
    10021 gtagaagcca gtttgatcag aatacgatcg ttgctaatac ctgcatcgtt gtagagtttg
    10081 atcaggcgtt ttgctttcgc aattgacgct tcagtgtcat aggaaagacg cgcatcaact
    10141 tcagttgaga tacggcccgg aaccagtttc aggatttcca gaccaatatt tactgccagt
    10201 ttgtcggtcg cgtccacgat ctgctgcgcg cgatcgttgc tctgctgttt cgcccaggcg
    10261 acagcatcat caatcaactt acggtattcc ggaatctgcg ctgcgttaag aatgagagaa
    10321 gggttggttg tggcatcctg cggttgatac agcttcattg ccgcgatgtc cccagtgtcg
    10381 gccactacgg tggtgtactg acgaagggag gtcaatttgt ccgtcatgat agtatttctc
    10441 tttaaacagc ttgttagggg gatgtaaccg gtctgccctg atgatatcac gacgcaacgt
    10501 cagcgcaacc gccgacaaga cggttatcgc caggcgagac tgtttcggat ttctgacact
    10561 gcgttgatat cgcccaccat ttttatacaa aacctcatgt atgctacgca gaagttatca
    10621 agtacctcgt agcgtatata cttcttaaac aattggtaac gtttacacag gaaagtcatc
    10681 gcgaccggca ataagaggga tatgcatgcc agattttttc tcctttatta acagcgtcct
    10741 ttggggatcg gtaatgattt acctgctctt cggcgcaggt tgttggttca cttttcgcac
    10801 cggatttgtg cagtttcgct acatccgcca gtttggcaaa agtcttaaaa atagcattca
    10861 tccacagcca ggcggtttaa cctcatttca gtcattgtgt accagtcttg cggcgcgcgt
    10921 gggtagcggc aacctggccg gcgttgcgct ggcaattacc gccggtggac caggtgccgt
    10981 cttttggatg tgggttgctg cgtttatcgg catggcgacc tcgtttgccg aatgttccct
    11041 tgcacaactt tataaagaac gtgacgtcaa tgggcagttt cgtggcggac cggcatggta
    11101 tatggcgcgc gggctgggga tgcgctggat gggcgttctg ttcgccgtct ttttgctcat
    11161 cgcctacggc ataattttca gcggagttca ggcgaacgcc gttgcccgag ccctgagttt
    11221 ttcttttgat tttcctccgc tggtgacggg cattattctc gctgtctttg ctctgctggc
    11281 aatcactcgt ggtcttcatg gcgtcgcccg gctcatgcag gggtttgtcc cgttgatggc
    11341 gataatctgg gtactgacca gcctggtgat ttgcgtaata aatatcgggc aacttcccca
    11401 cgtcatttgg tctatttttg agagtgcttt tggctggcag gaagcggcag gcggcgcggc
    11461 gggatatacc ttaagccagg cgattactaa cggttttcag cgcagtatgt tttccaatga
    11521 ggtgggaatg ggatcgacgc caaacgcggc agcggcagct gcatcctggc ctccgcatcc
    11581 ggcagcacaa gggattgtcc agatgattgg catttttatc gacaccctgg tcatctgtac
    11641 ggcaagcgcc atgctgatat tactggcggg taacggcaca acctacatgc cgctggaagg
    11701 tattcagctt atccagaagg cgatgcgggt gctaatgggt tcctagggtg ctgaatttgt
    11761 taccctcgtg gttattctgt ttgccttcag ctccatcgtt gccaactaca tttatgccga
    11821 aaacaatctc ttctttttac gcctgaacaa ccctaaagcg atctggtgtt tgcggatctg
    11881 caccttcgca acggtcatcg gcggcacctt gctaagcctt ccgctgatgt ggcaactggc
    11941 agatatcata atggcctgca tggctattac caatttgacc gccattttac tgctctcgcc
    12001 tgtggttcat accattgcca gtgattatct acgccagcgt aaactcggcg tgcgcccggt
    12061 gtttgatccg ttgcgttatc cggatatcgg tcgccagctt tctccggacg cgtgggatga
    12121 tgtttcgcag gagtaatcac aactatcgat caactcattc tctttttttg ctaaagtcgg
    12181 cataaatttc ctgcaaggac tggatatgct gattcttatt tcacctgcga aaacgcttga
    12241 ttaccaaagc ccgttgacca ccacgcgcta tacgctgccg gagctgttag acaattccca
    12301 gcagttgatc catgaagcgc ggaaactgac gcctccgcag attagcacgc tgatgcgcat
    12361 cagcgacaaa ctggcgggta tcaacgccgc tcgctttcat gactggcagc cagatttcac
    12421 gccggcgaat gcccgccagg cgattctggc gtttaaaggt gatgtctaca ccggcttgca
    12481 ggccgaaacc ttcagcgaag acgatttcga ttttgcccaa cagcatttgc gaatgctttc
    12541 cggcttgtat ggcgtactgc gcccgctcga tttaatgcag ccttatcgtc tggaaatggg
    12601 gatccgtctt gagaatgccc gagggaaaga tctgtatcaa ttctggggag atatcatcac
    12661 caacaagctg aacgaggcgc tcgcagcaca aggcgataat gtggtgatta atctggtgtc
    12721 agatgagtat tttaaatcgg tgaagccgaa gaaattgaat gccgagatta tcaagccggt
    12781 gttcctcgat gagaagaacg gcaaatttaa gatcatcagc ttctacgcta agaaagcacg
    12841 cggtctgatg agtcgtttca ttattgaaaa tcggctgacc aaaccagagc aactgactgg
    12901 ttttaatagc gaaggttact tctttgatga agattcctcc agcaatggcg aactggtgtt
    12961 taaacgctac gagcagcgtt aagtttgtag tcaatgccgg atgcggcgtg aacgccttat
    13021 ccggcctaca aaagcatgca gattcaatat attgcagaat taacgtaggc ctgataagac
    13081 gcgccagcgt cgcatcaggc gctgaatgcc ggattgttac ccggcatttg tcatttagcg
    13141 gtgatgtttg cctggaccat gaccgccgtg atgatcatga ggagctttct tatggtggcg
    13201 cggcggtggc ggcggtccgt gtgggtgcca gcgattgcct cgccattcat aatgttgttt
    13261 ccaccagccg tggtcgcgcc agtgacctcc atcccagtaa tagccacgat tatcacgatc
    13321 gcctatctgt aattttactg acgggactaa cgtaatttcc gcagcctgtg ctgccatggg
    13381 agcgaccaga accagggaaa gtgcgagtac gatagattgc atctttttca caggttactc
    13441 cttttatcat tgcccgttat gggtcgatat ccggagatta cgcgatgaga acgacggttg
    13501 ttattctccg caatcctaat ctctaagtta ccgcatttat tgataatttc tcctttttcc
    13561 ctgtcgtttt tctccataat ttcttcataa aaaagccggg ctgcataaaa gcaaacccgg
    13621 cctgattgag ataatgaata gattttactg atgattcatc atcaatttac gcaacgcaga
    13681 aaaatcggcg ggcagattat gtgaaagcaa gggtaaatca gcacgttctg ccagctcttt
    13741 tggcagatcc aacgtttcac cgagaatcgc ttccacgctc tctttaaatt tcgccggatg
    13801 cgcggtgccg aggaacaagc catattcgcc tggattcaac tggtcacgca gcgcacgata
    13861 cgctacggca gcgtgcggct ccgaggtgta gcccagttct tttaactcac gcattgtctg
    13921 ttgcgtggtt tcatcatcca cggctgcata acccagctct ttcagttgcc agattttgcg
    13981 gcggaacaac tcttccacac gcggccagtt gttcggctga ctcacgtcca tcgcgttgga
    14041 taacgtcgcc tgagtcgctt tgggtgacca ctgaccgtcg tgcaggaaac gtggcacggt
    14101 atcgttcacg ttggtcgcag caataaaacg tttcaccggc agaccgaatg acttcgccag
    14161 cagacccgcc gtcaaatcgc cgaagtttcc gcttggcacc gagacaacca gctggttgcg
    14221 cgcctcctgc ggcagctgcg caacagcttc aaagtagtag caaatctgcg ccagcaaacg
    14281 gctgatgtta atcgagttag ccgagtttaa ccccagcgcc actttcagtt cttcatcatc
    14341 aaacgcctgc ttcaccagcg cctgacaggc atcgaaatcg ccgtcgatgg caacagtttc
    14401 gatattgccg cccaacgtac agaacagttt ttcttgcagt ggactgattt tgcctcgtgg
    14461 atagaggata accactttca cattcggcaa accgtagaaa gcatgagcca ctgcagctcc
    14521 ggtatcaccg gaggtcgcgg tcagaatggt cactggctta tcatccgcaa tatgggtcag
    14581 catttgtgcc ataaagcgac cgccgaaatc tttaaatgcc agcgttggcc cgtggaacaa
    14641 ttccagacaa ccgacatcgc tttcaacatt ggcgaccgga gccgggaagg caaacgccgc
    14701 gcgcacgcgc tcttccagga tttcctgtgg gatttcatca ccaataaacg ccgagaggat
    14761 cttcgcactg cgggtgacaa aatccagctt cagcatctca tcaatttcag tcaggctgaa
    14821 ttccggcagg tcgtgcggaa aaaacagccc ctgatttttg cccaacccct gggttacggc
    14881 ttgcgcaaag ctgacctgct cgttgtgatc tttcagattg tagagtttca tttagttttc
    14941 cagtactcgt gcgcccgccg tatccagccg gcaaatatga acaaaacctt cctgattttg
    15001 caggtagttc ttacccaacc agtcggcaac gcgctgggcg gtatccggct tgtcacatag
    15061 agcgaacaag gtcgggccgg agccggagat accgctcgct accgcgccga tttccgcgac
    15121 cgcctgccgc gcctgccgga agccaggcag taaccgttca cggtagggtt cagcgataac
    15181 atctttcatc agcttcgcgg caagctcagg ctgacgggaa tagcaggcgt gaatgaagcc
    15241 agccagatgt cgcccgtgcg caatgcaatc ctggcggcga tactgcgccg gtaaaatagc
    15301 ccgggcttct gccgtcgaga ctttaattcc cggatacgcc agcacccaca gccactcatc
    15361 aaaccctggc acttgctggc tgatgatgtc gttttcttcg atcatcaact gcataccacc
    15421 aagaaaacac ggtgccacgt tgtcgtaatg aatgctgccg gagatacgtc cttccagctc
    15481 gcccatcaaa gccagcaaac gagtgtcatt aagcggcttg ccgcagtgtt cattcatcgc
    15541 catcagcgcc gcgaccaccg aacaggcgct ggagcctaag cccgaaccga tcggcatatt
    15601 cttttccaga gtcatcgcca ctggaatttg cttgccaagc tcctggcaaa aacgctccca
    15661 gcactgataa acgatatttt cccgtggttc tgacggcagc ttatcggcaa agcgtccgag
    15721 tttgttgaga ctgaatgtct ctgccgcctc aaccgtgact acatctccga gcaatgcacc
    15781 atcaacgggt gccaccgccg ccccgagcac atcaaacccg acgctcatat tggcactgga
    15841 agccggggca taaactttaa ccatgtcaga ctcctaactt ccatgagagg gtacgtagca
    15901 gatcggcaaa gacaccggca gcggtaacgt cattgcccgc accatatccg cgcagcacca
    15961 acggcagcgg ctgataatag tggctataaa aggccagggc gttttcgcca tttttcactt
    16021 tgaacagcgg atcattccca tccacttcgg caatcttcac gcggcagaca ccatcttcat
    16081 caatattgcc aacatagcgc aaaacttttc cttcatcacg ggccttcgcc acgcgcgcgg
    16141 caaagagatc gtcgagctgt gacagattcg ccataaaagc ggcaacatca ccctcggcgt
    16201 taaactctgc gggcagcaca ggttcaattt caatatccgc cagctccagt tcacgtcccg
    16261 tttcacgagc gagaatcaat agtttacgcg ccacatccat accagaaaga tcatctcgcg
    16321 ggtccggttc ggtataaccc atttcccgcg ccagcgtggt cgcctcggag aaactcatgc
    16381 cttcgtctaa cttgccgaag atataagaaa gcgaacctga aagaatgccg gagaacttca
    16441 ccaattcatc acctgcattg agcagatttt gcaggttctc aataaccggt aatccagccc
    16501 caacgttggt gtcatagagg aatttacgcc gcgatttttc cgccgcataa cgcaactgat
    16561 gatagtaatc catcgacgag gtgttggcct ttttgttcgg cgtgacaacg tggaaacctt
    16621 cgcgcaggaa gtcggcatat tgatccgcca ctgcctggct ggaggtgcag tcaacaatga
    16681 ccgggttcag cagatgatat tctttcacga ggcgaattaa gcgcccgaga ttaaacggct
    16741 ctttggcttg tgcaagttct tcctgccagt tttccagatt aaggccatgc acactagtga
    16801 gcagagcctt cgagttggca acgccgcaga cacgtaagtc gatatgttta ttcttcagcc
    16861 agctttgctg acgcttcagt tgctccagca gcgcaccgcc aacgccaccg acgccaatca
    16921 caaacacttc gataacctga tcggtattga acagcatctg atgagtaacg cgcacgccag
    16981 tggtcgcatc atcgttattt accacgacag agattgagcg ttcagaagat ccctgagcaa
    17041 tggcgacaat gttgatattg gcgcgggcca gcgcggcaaa gaatttcgcc gagatcccac
    17101 gcaaggtgcg cataccatca cctaccaccg agataatggc cagccgttcc gtcaccgcca
    17161 gcggctccag taagccttct ttcagttcca ggtagaactc ttcctgcatt gcccgttcag
    17221 ctcgcacaca gtcgctttgt ggaacgcaga aactgatact gtattcggaa gatgattgcg
    17281 taatcagcac cacggaaata cgggcgcgtg acattgcagc aaagacgcgc gccgccatgc
    17341 caaccatccc tttcatcccc gggccggaaa cgctgaacat tgccatgtta ttcagattgg
    17401 aaatgccctt gaccggtaat tcgtcttcat cacggctggc accaatgagc gtacctggtg
    17461 cttgaggatt tccggtattt ttaatcaggc aagggatctg gaactgggcg atgggggtaa
    17521 tggtgcgggg gtgaagaact ttagcgccga agtaggaaag ctccatcgct tcctggtagg
    17581 acatcgactt caacaacctc gcatcgggca cctgacgcgg gtcgcaggta tagaccccgt
    17641 caacgtccgt ccaaatctcg caacaatcgg cgcgtaaaca ggcagccagc accgccgcgg
    17701 agtagtcgga accgttacgt ccaagcacca ccagttcgcc tttttcatta ccggcggtga
    17761 aacctgccat caacaccatg tgatcagccg gaatgcggct tgccgcaata cggcgggtgg
    17821 actcagcaat atcgacggta gattcgaggt aatgccccac tgccagcagt ttttcgaccg
    17881 gatcgataac ggtaacgttg tgaccacgcg cttctaacac gccggccata atggcgatcg
    17941 acattttctc gccacggcaa atcagcgcag cgttgatgct atccgggcac tgccccaaca
    18001 aactaatgcc atgcaggaca tgttttattt gggcaaattc ctgatcgacg aaagttttca
    18061 attgcgccag cgggaacccc ggctgggcgg cggcgagtcc cgtcaaaagt tcggcaaaaa
    18121 tacgttcggc atcgctgata ttgggtaaag catcctggcc gctaatggtt ttttcaatca
    18181 tcgccaccag gtggttggtg attttggcgg gggcagagag gacggtggcc acctgcccct
    18241 gcctggcatt gctttccaga atatcggcaa cacgcagaaa acgttctgca tttgccactg
    18301 atgtaccgcc gaacttcaac actcgcatgg ttgttacctc gttacctttg gtcgaaaaaa
    18361 aagcccgcac tgtcaggtgc gggctttttt ctgtgtttcc tgtacgcgtc agcccgcacc
    18421 gttacctgtg gtaatggtga tggtggtggt aatggtggtg ctaatgcgtt tcatggatgt
    18481 tgtgtactct gtaattttta tctgtctgtg cgctatgcct atattggtta aagtatttag
    18541 tgacctaagt caataaaatt ttaatttact cacggcaggt aaccagttca gaagctgcta
    18601 tcagacactc tttttttaat ccacacagag acatattgcc cgttgcagtc agaatgaaaa
    18661 gctgaaaaat acttactaag gcgtttttta tttggtgata tttttttcaa tatcatgcag
    18721 caaacggtgc aacattgccg tgtctcgttg ctctaaaagc cccaggcgtt gctgtaacca
    18781 gtcgaccagt tttatgtcat ctgccactgc cagagtcgtc agcaatgcca tgactcgttc
    18841 gcgtaaagct tgcagttgat gttggtctgc cgttgtgtca cttttcgccg gttgttgtat
    18901 taatgttgct aattgatagc aatagaccat caccgcctgc cccagattga gcgaaggata
    18961 atccgccacc atcggcacac cagtaagcac gtcagccaac gctaactctt cgttagtcag
    19021 cccggaatct tcgcgaccaa acaccagcgc ggcatggctc atccatgaag atttttcctc
    19081 taacagcggc accagttcta ctggcgtggc gtagtaatga tatttcgccc gactacgcgc
    19141 agtggtggcg acagtgaaat cgacatcgtg taacgattca gccagtgtcg ggaaaacttt
    19201 aatattatca ataatatcac cagatccatg tgcgacccag cgggtggctg gctccaggtg
    19261 tgcctgacta tcgacaatcc gcagttcgct aaaacccatc gttttcattg cccgcgccgc
    19321 tgccccaata ttttctgctc tggcgggtgc gaccaggata atcgttatac gcatattgcc
    19381 actcttcttg accaaataac cgcgaacagg gtgatcactg tcaacttatt acgcggtgcg
    19441 aatttacaaa ttcttaacgt aagccgcaga aaaagccctt tacttagctt aaaaaaggct
    19501 aaactatttt ctgactgcac taacggttta attgttaaaa aatgctacat atccttctgt
    19561 ttacttagga taattttata aaaaataaat ctcgacaatt ggattcacca cgtttattag
    19621 ttgtatgatg caactagttg gattattaaa ataatgtgac gaaagctagc atttagatac
    19681 gatgatttca tcaaactgtt aacgtgctac aattgaactt gatatatgtc aacgaagcgt
    19741 agttttattg ggtgtccggc ccctcttagc ctgttatgtt gctgttaaaa tggttaggat
    19801 gacagccgtt tttgacactg tcgggtcctg agggaaagta cccacgacca agctaatgat
    19861 gttgttgacg ttgatggaaa gtgcatcaag aacgcaatta cgtactttag tcatgttacg
    19921 ccgatcatgt taatttgcag catgcatcag gcaggtcagg gacttttgta cttcctgttt
    19981 cgatttagtt ggcaatttag gtagcaaaca tgcagacccc gcacattctt atcgttgaag
    20041 acgagttggt aacacgcaac acgttgaaaa gtattttcga agcggaaggc tatgatgttt
    20101 tcgaagcgac agatggcgcg gaaatgcatc agatcctctc tgaatatgac atcaacctgg
    20161 tgatcatgga tatcaatctg ccgggtaaga acggtcttct gttagcgcgt gaactgcgcg
    20221 agcaggcgaa tgttgcgttg atgttcctga ctggccgtga caacgaagtc gataaaattc
    20281 tcggcctcga aatcggtgca gatgactaca tcaccaaacc gttcaacccg cgtgaactga
    20341 cgattcgtgc acgcaacctg ctgtcccgta ccatgaatct gggtactgtc agcgaagaac
    20401 gtcgtagcgt tgaaagctac aagttcaatg gttgggaact ggacatcaac agccgttcgt
    20461 tgatcggccc tgatggcgag cagtacaagc tgccgcgcag cgagttccgc gccatgcttc
    20521 acttctgtga aaacccaggc aaaattcagt ctcgtgctga actgctgaag aaaatgaccg
    20581 gccgtgagct gaaaccgcac gaccgtactg tagacgtgac gatccgccgt attcgtaaac
    20641 atttcgaatc tacgccggat acgccggaaa tcatcgccac cattcacggt gaaggttatc
    20701 gcttctgcgg tgatctggaa gattaatcgg ctttaccacc gtcaaaaaaa cggcgctttt
    20761 tagcgccgtt tttatttttc aaccttattt ccagatacgt aattcatcgt ccgttgtaac
    20821 ttctttactg gctttcattt tcggcagtga aaacgcatac cagtctatat tacgagtcac
    20881 aaacatcatg ccggccagcg ccaccaccag cacactggtt cccaacaaca gcgcgctatc
    20941 ggcagagttg agcagtcccc acatcactcc atccagcaac aacagcgcga gggtaaacaa
    21001 catgctattg cgccagcctt tcaataccgc ctgcaaataa ataccgttca ttaacgcccc
    21061 aatcagactg gcgattatcc atgccacggt aaaaccgata tgttcagaaa gcgccagcaa
    21121 gagcaaataa aacatcacca atgaaagccc caccagcaaa tattgcattg ggtgtaaacg
    21181 ttgcgcggtg agcgtttcaa aaacaaagaa cgccataaaa gtcagtgcaa tcagcagaat
    21241 ggcgtactta gtcgcccggt ccgttaattg gtattgatcg gctggcgtcg tcactgcgac
    21301 gctaaacgcg gggaagtttt cccagccggt atcattgcct gaagcaaaac gctcaccgag
    21361 attattagca aaccagctgc tttgccattg cgcctgaaaa cctgactcgc tagcttcccg
    21421 tttggctggt agaaaatcac ctaaaaaact gggatgcggc cagttgctgg ttaaggtcat
    21481 ttcgctatta cgcccggcag gcaccacaga aagatcgccg gtaccgctta aattcagtgc
    21541 catattcagc tgcaggttct gcttccgcca gtccccttcg ggtaaaggga tatgcacgcc
    21601 ctgcccgcct tgctctaacc cggtaccggg ttcaatggtc agtgccgttc cgttaacttc
    21661 aggcgctttc accacaccaa taccacgcgc atccccgacg ctaatcacaa taaagggctt
    21721 gcctaagatg atatttggcg cgttgagctc gctaagacgc gaaacatcga aatcggcttt
    21781 tagcgttaaa tcactgtgcc agacctgacc ggtataaatc cctatcttgc gttcttccac
    21841 gttctgattg ccatcaacca tcaatgactc aggtaaccaa aaatggataa aacttcgttt
    21901 ccgctccacg gttttatcct cttcctgcac cgtataaagc tcggtcacag gaatagcgat
    21961 aagcggccca acgagttttt gcggcccgct ggtactttgg cgaatcgcat cttccacatc
    22021 gctacggtaa tcggcacgtt cgacaatcac ttgccgaatc agcattatcg gaatcaacaa
    22081 cagcaatact gcaccaaaca aggtggtcat tttccagaac aggggggatt tcaacatagt
    22141 cttctccttt gcaatggcag caggatacga aggctatgtg ggaagaattt gaagctatgt
    22201 gaagtgacgg tgaagtcgta gcgaggccag cacgccacct tcctgcacgt tgcgcagcgt
    22261 gacttcgccg ttaaacaaac gggcgacctc actgacgaac gccaacccca gaccgctgct
    22321 tttttgccca tttgcacgcg gcaaagagta aaagcgttca aaaatacgtg acagcgcgta
    22381 gtcaggaata ccactaccgg tatccagcac cttaagcgtg acgtgttcct gatccacttc
    22441 ggcgcttagc gttatgcaac cgctctcggg agtaaaatcg atggcgttat ccagtaaatt
    22501 ccccagcgcc tgatccagta acgccggttc agcagcaaca ttaacctcgg tgggcataac
    22561 atgcaaagtg atgttttttt ctgccaactg cacggtgcgc gcttcgctga cgcggcgaaa
    22621 taatgccgcc acatcaacag cagtcagaac gacttcctga cgattctcca gtcttgcctg
    22681 gcgtagtaac gtttccacca gcgcctgcat tcgcgcattt tgtgtcagaa tgttgtcagt
    22741 aaaacgagcc actacttcag gcggcggacc ttcgcgtaaa atttccgccg cgccacgaat
    22801 cgccgccagt gggcttttta gctcatgggt taacgcataa acatactgct caatatagtt
    22861 tttcccttcc agcttcacgc gcatactttc cagcgcctgc gcgagtttac gcagctcgct
    22921 actaccgaga tcgggaagag gaacgggctt attgtcagtg acggaatcag catagcgagt
    22981 gagccgggca atggagcggt tgatccacca aaccatgcct gcgccaatca ccagtgcaat
    23041 ccccaacaaa atggcgctgg cccataaaat tcgccgctcg ctgcgtttaa tgaccggagc
    23101 catcgccgcg ttcggtttgc ctacgctcaa aacgccaata agccgcgagc cgcccataat
    23161 cggcgcggca acatacatca ccgaactttc gggatcggca ggattttgca acgtgctgcg
    23221 cgcaccatac tgaccacgca acgttagcca gacgtcattc cagcgcgaat aatcctgtcc
    23281 aacggcttta tttgccgaat cgaacaatac tttgccatgc gcatcggtca tatagacatg
    23341 atattcattg cgcactttgt taatgccacc aatattggcg cgaaacgggc gatgttgtag
    23401 ctgattaaac gcctgcgcca gttgcccatg cgttgggtcc ccagagagca aatcgggacg
    23461 cgccagctcc gcaagtaacg ttgccgtgtc gatcaacgtc ccctccgttg ctcttcgcac
    23521 gcccggttta acttctttga caaaaatagc cagtacgaac caggccgcca ccgccaccag
    23581 taaaaaatag cccagcagca accgcatgcc gatacgcatt acaagcccct caggctatat
    23641 cccatgccgc gatgagtatt aatcggtgaa agatcggggt tgatggcgcg cagcttggca
    23701 cgcagcgttt taatgtggat atcgacggtg cgatcgtagg tgtcctgcgc atcttcccac
    23761 acgctatcca ttagttgctg gcgggaccag acgcggcctg gcgacttgag taacgtcttc
    23821 agcaataaaa actcataccg cgtcagcgtt aatggcgtgt caaaccagct gatctgcgcc
    23881 gcgggttcat tcaattcaaa atgaccaata cggatgacgg gagacggcgt cgagaacttc
    23941 ttcacccgac gcagtaaggt gcgcaccctg gcgcacactt cgcggggtga aaacggttta
    24001 gccacatagt cgtcagcgcc aatttccagc ccaagcaggc gatcgacctc ttcacttcgg
    24061 gccgtcagga acagtacagg taacgccgga tggagcgcca gtaactggcg gcacaattca
    24121 aagccactga tatcaggcaa accaacgtcg agaatcatga cgtcgggaac ctgctggcga
    24181 gctttatcca gcaccggcaa acctcgctca aagacctcga cggcaaaacc ttcctgctgc
    24241 aacatgtaga ccagcgtgtc ggctatccct tgctcatctt ccactaacca gaccgtttcc
    24301 cgttgcataa atcgcctctg ttattgccgc cacggcatga caggaaccgc gctaatcgcg
    24361 tttttcggcg aaccttctac aactttgtcg gagtaagcca gataagcgag cgcgttgcgt
    24421 ttggcgtcat aaaagcgcac gacctgtaac gacttaaaga ccagggacgt gcgttttttg
    24481 aatactacct cgccctgagc tttgccgttt ttaatacgat ccgacagttc aatcggcccg
    24541 acttgctgac aagaaatggc cgcatcggag gtatcttctg ccaggcccaa tcccccttta
    24601 ataccaccgg ttttcgcccg gctcacataa caggtgacat ttttcacatc gggatcgtca
    24661 aaggcttcca caacaatttt gtgatccggg ccgatcattt taaatacggt gtcgaccgaa
    24721 ccaatctctt ctgcatgagc caatggcccc agcattatca ggcttaaaga caggatcaaa
    24781 tgcttgtatt tcatattgtt accattttta tctattacac gagcaattag ttcgtcacgg
    24841 taaataacat attgattaag aacaaaatct caattctttt atttccgtca agccctaaaa
    24901 catactctac taagaaaaaa acactgaatg ctaaaacagc aaaaaatgct attatccaat
    24961 tacctgatgt caggtgctcg ttgttgaaag gatgaggata ttttatggat caggccggca
    25021 ttattcgcga ccttttaatc tggctggaag gtcatctgga tcagcccctg tcgctcgaca
    25081 atgtagcggc gaaagcaggt tattccaagt ggcacttaca gagaatgttt aaagatgtca
    25141 ctggccatgc tattggcgcg tatattcgtg ctcgtcgttt gtcgaaatcg gcggtcgcac
    25201 tacgcctgac tgcgcgtccg attctggaca tcgcgctgca ataccgcttc gactctcaac
    25261 agacatttac ccgcgcattc aagaagcagt ttgcccagac tcctgcactt taccgccgtt
    25321 ctcctgaatg gagcgccttt ggtattcgcc cgccgctacg tctgggtgaa ttcacaatgc
    25381 cagagcacaa atttgtcacc ctggaagata cgccgctgat tggtgttacc cagagctact
    25441 cctgttcgct ggagcaaatc tctgatttcc gccatgaaat gcgttatcag ttctggcacg
    25501 attttctcgg caacgcgccg accattccgc cggtgctcta cggcctgaat gaaacgcgtc
    25561 cgagtcagga taaagacgac gaacaagagg tattctatac caccgcgtta gcccaggatc
    25621 aggcagatgg ctatgtactg acggggcatc cggtgatgct gcagggcggc gaatatgtga
    25681 tgtttaccta tgaaggtctg ggaaccggcg tgcaggagtt tatcctgacg gtatacggaa
    25741 cgtgcatgcc aatgctcaat ctgacgcgcc gtaaaggtca ggatattgag cgatactacc
    25801 cggcagaaga cgccaaagcg ggagatcgcc caattaatct gcgctgtgaa ctgctgattc
    25861 ctatccgccg ttaacgctgc agctcatcta atgcaggggc gtccagatgc gagatgtccc
    25921 ctgcggtttc aacgacccag ccagacgcca gccacaggct ttcctgataa tccacgcgcg
    25981 aaatagaaca gttacgcaga cgtaagcggc gctctgccca tgctggtaat ccgagaatcg
    26041 tactcaccag acatcccagt gcaataccat ggctcaccag cagcggtcgg cttccctgcg
    26101 gtaagtcccg gcaagattcc agtgccgcat tgacgcgatc gctgagctcc tgcattgact
    26161 cgccttcagg aatacgcccg tcaacggtgc cattgaccag ctgccgacgc cagttctctt
    26221 cttcttcggt cagagaatcg atatgtcttt tttccagcac acccatgttt aattcacgca
    26281 ggcgagaatc aaagatgatg tcacagccgc aggcctgggc gatgatttcc gccgtacgcc
    26341 gggtgcgtcc taaatcgcta ctgataatat gcgtaatgcc aagctctttg gcacgggttg
    26401 ccacctgcat cgcctgttgc tcacctttgg cggtcagcgg gctgtcagac tggccctgaa
    26461 tacgtcgctc ggcgttccac tgcgtttcac cgtggcggac taggtatacc tgtaacatgc
    26521 tttttttccg tatactgctg taacgttaat tttctgagta atgctgatta tgcaccaagt
    26581 tgtctgtgcg accaccaatc ccgctaaaat tcaggccatt ctgcaggcat ttcacgagat
    26641 cttcggcgaa ggatcctgcc atattgcatc cgttgccgtc gagagcggtg taccggaaca
    26701 accctttggc agtgaggaaa cgcgcgctgg cgcacgaaat cgggtagcaa atgcccgccg
    26761 tttacttcca gaggctgatt tttgggtggc gattgaagct ggcattgatg gcgacagcac
    26821 tttcagttgg gtggtgattg aaaacgccag ccagcgcggc gaggcgcgtt cggcaacttt
    26881 gccgttacct gcggtgattc tggaaaaagt tcgtgaaggc gaggcgcttg gtcccgtgag
    26941 tcgcgttata ccggtattga tgagattggt cgtaaggaag gggcgatcgg cgtgtttacc
    27001 gccgggaaac tcactcgcgc cagcgtctat caccaggcgg tgatcctggc gctgagtccg
    27061 tttcacaatg ccgtgtatca ggcattgtag gacggataag gcgttcacgc cgcatccgac
    27121 attcggtgca cgatgcctga tgcgacgctg ccgcgtctta tcaggcctac aaaatcaatc
    27181 gcttttcagc aacacctctt ccagccactg acgcagctcc acgggcgcgg ctttcaggct
    27241 gttagatcca cgcgtaatcg tcgcgatgcc cgcgccgagt tcatttttta actcacgctg
    27301 actcatttcg ccacgcaaca gctcttcgac aatacgcacg cgagtcccca acgcttcgcg
    27361 ctcatctggc gtcagcatca ggtttaacaa cggtaaatgg agatcgtttt ggtaggcatt
    27421 cttaagcagg tcgacaaaac gtaaccactc ctggtgacgc tgttctgcca tcgctgctga
    27481 atagggtgat tgttgggcca taatatgtcg ccattgttag cgggggaagc aaaatgactc
    27541 ccccggttgt actcgctaaa gagtacgata gcatatcata aacgtgcgga tcagtaacga
    27601 cgtccccatt ccgtggcgct catcaacgtc ggtttatccc ccatgaaata gcggtagtaa
    27661 gcatcataag ccagcacgtt cttcacataa ccgcgcgtct cggagaacgg aatactctcg
    27721 acaaatgcca cagcgtcgat acgcccggcg ctgttgccaa gccaggttcg cacccgtcca
    27781 ggtccggcgt tataagctgc tgaggagaaa atacggttat tgccaaactg ctgataaaca
    27841 tattgcaggt aactggtgcc aatgttgatg ttcgtttccg gatccagcaa ttgcccaggg
    27901 ctgctataac caggaataga gaacatcttc accgtatggg tcgctgtacc aggcataatc
    27961 tgcatcaggc cgctggcccc taccggtgat ttcactttcg gattccaggc gctctcctga
    28021 cgggcaatcg ccatcgcata gctttgcggg atctctttcc cactggtgta acgtttgaaa
    28081 agatcgttgt aagccagcgg gaatcgctct tccagatgat cccaaagctt cccggcgatc
    28141 gttgcctgaa cgctaagatc ccaccattgg ttattgaaag cataccgagc cagttgagcc
    28201 tgctctgttt ttgacttgct cttcaccaga ttggcccact cgctacgcgc ggtattatcg
    28261 agattccagt acatcaactc gcgcacgcgc gccatctccg gcccctgagt cagggtgctg
    28321 tcaacattct gcggcgcttt atcaatcttc agctcatact cttcaccgat gcgttgtgct
    28381 gcaaccatcg ggtagaaacc acgctgttgt atgagttgat gcaaaatctc tttcgcttca
    28441 gcttcacgtc cgcgttccag caataaatcc gcctgccagt aacgccattc atctttctct
    28501 tttgcttcca tcggcagacg cgccagccag gtattcaggc cgcgacgatc gccggtgcca
    28561 agcgccattc gcacacggcg ttcaatcaac gacgtggatt gcgagcgcat aatggcgtca
    28621 tcgcgccatt tcgcctgctc atcggtgaca tcattgccca tcaaacgcca ggcgacgata
    28681 tcgcgcagct cctgaatctg atcttcatta agctgctgcg cctgggcaag cgacgggatc
    28741 atcagccgtg cattttcggc atcctgccgc gccacactgg caaacgccac cgccgccatt
    28801 tgacgggtaa aatcggtcgc gccggttgta cgcgcgaagg tcagtaccgt attagggttg
    28861 ttcgccagtg aaatgattgc cgaggcgata gtctggtaat cggcaggcat ctgccctgcc
    28921 agcactgtta ccaggcctgt gttacccgct ttcatcgcca gacggatacg ctctaaatac
    28981 gccagcggat cttgtttacc tgacgcacgc cagacgctaa ataacttgtc acaggcgtta
    29041 ggctggctct tgccggttag ccacagctct ttcgcccctt gccaggcttc ttcactctgc
    29101 ccggtgttcc atttcgcata gtagtaatta cattgcgctt cggtagttcc gggcttttcc
    29161 gggctaaagg ctaacaagcc acgccagtct tcacgccgcg ccagttcatt gacgaaacga
    29221 gattgcagcg tgcgagcggg aggaagcgtg gggttagcgc gaacaaagtt agtgaccgtc
    29281 accgccggtt gattcatcag gtcatcggtg atctggcggt attccaggta gggataaagc
    29341 ggataatcct tcagtccagg catcatttgt tcgaccacat ccatctgtcg attatcccag
    29401 gcctgcttga tttgcgcgta acggctacgc tgctcatcca gtgagtcggc tcgcgccacg
    29461 ctgctgaccg tcagcagaca gacaccggca gccaacagcc gccaggtaac ttgtttggct
    29521 ttttccacaa gcgcatcctc taagtgtaaa tacatcaatg cagcgtcatg ccgcgtaata
    29581 gatttatgct aaccagtcat tgccgtttac gccacgttac ggacactttt ttacttttac
    29641 tgcgaaggtg atcgaccaac tgggattagg cggggaaaca ggctacactt cgcctttgaa
    29701 aactcatcat cacgataaac atagaggcga agtccaacgt ggctcaattc gtttatacca
    29761 tgcatcgtgt cggcaaagtt gttccgccga aacgtcatat tttgaaaaac atctctctga
    29821 gtttcttccc tggggcaaaa attggtgtcc tgggtctgaa cggcgcgggt aagtccaccc
    29881 tgctgcgcat tatggcgggc attgataaag acatcgaagg tgaagcgcgt ccgcagccag
    29941 acatcaagat tggttatctg ccgcaggaac cgcagctgaa cccggaacac accgtgcgtg
    30001 agtccattga agaagcggtt tcagaagtgg ttaacgccct gaaacgcctg gatgaagtgt
    30061 atgcgctgta cgccgatccg gatgccgatt ttgacaagct ggccgctgaa caaggccgtc
    30121 tggaagagat cattcaggct cacgacggtc ataacctgaa cgtacagctg gagcgtgcgg
    30181 cggatgcgct gcgtctgcca gattgggacg cgaaaatcgc taacctctcc ggtggtgagc
    30241 gtcgtcgcgt ggcgttgtgc cgcctgctgc tggaaaaacc agacatgctg ctgctcgacg
    30301 aaccgaccaa ccacctggat gccgaatccg tggcgtggct ggaacgcttc ctgcacgact
    30361 tcgaaggcac cgtggtggcg attactcacg accgttactt cctcgataac gttgcaggct
    30421 ggatcctcga actggaccgc ggtgaaggaa ttccgtggga aggtaactac tcctcctggc
    30481 tggagcagaa agatcagcgt ctggcgcagg aagcttcaca agaagcggcg cgtcgtaagt
    30541 cgatcgagaa agagctggag tgggtacgtc agggaactaa aggccgccag tcgaaaggta
    30601 aagcacgtct ggcacgcttt gaagagctga acagcaccga ataccagaaa cgtaacgaaa
    30661 ccaacgaact gtttattcca cctggaccgc gtctgggcga taaagtgctg gaagtcagca
    30721 acctgcgtaa atcctacggc gatcgcctgc tgattgatga cctgagcttc tcgatcccga
    30781 aaggggcgat cgtcgggatc atcggtccga acggcgcggg taaatcgacc ctgttccgta
    30841 tgatctccgg tcaggaacag ccggacagcg gcaccatcac tttgggtgaa acggtgaaac
    30901 tggcatcggt tgatcagttc cgtgactcaa tggataacag caaaaccgtt tgggaagaag
    30961 tttccggcgg gctggatatt atgaagatcg gcaacaccga gatgccaagc cgcgcctacg
    31021 ttggccgctt taactttaaa ggggttgatc agggtaaacg tgttggcgaa ctttccggtg
    31081 gtgagcgcgg tcgtctgcat ctggcgaagc tgctgcaggt tggcggcaac atgctgctgc
    31141 tcgacgaacc aaccaacgac ctggatatcg aaaccctgcg cgcgctggaa aacgccctgc
    31201 tggagttccc gggctgtgcg atggttatct cgcacgaccg ttggttcctc gaccgtatcg
    31261 ccacgcacat cctggactac caggatgaag gtaaagttga gttcttcgaa ggtaacttta
    31321 ctgagtacga agagtacaaa aaacgcacgc tgggcgcaga cgcgctggag ccgaagcgta
    31381 tcaagtacaa gcgtattgcg aagtaatgcg taaaatgccg gatgcggcgc gaacgcctta
    31441 tccggcctac aaaaccagca atttcaatac gttgtaaaac tgtaggcctg ataagacgcg
    31501 gtaagcgtcg catcaggcat tgagcaccga atgccggatg cggcgtgaac accttttccg
    31561 gtctacaaca gccttagcat cctcaatttc gtggcgggtc acccgtgcag gttttttcaa
    31621 accttgttta atctcgacag cctcttccag agaggttttc aactcatcaa aaaaactcat
    31681 tttatacctc gcctttgagc agtttcgtca cggttatcct tgctccccca tcatctcccg
    31741 caccagctcc acgcagcgca ggaaacggct gtcgtaatcg tcctcttcaa cccgcacgaa
    31801 ttcgatattg ttctcttcca gtatctccac cagcaagttc tggaactctt tgcgatccac
    31861 cgaactgccg aggctgcgta aaccatccgc cacccacggc gtgttgttct ccagcaggat
    31921 caccagatcg aaacggtatt catcaatcag cgcctgcacg aacggatgct cacgcccttc
    31981 gtactttttg cagaacgcct gggtagtgac aaaatcggtg tcaataaacg ccactttatt
    32041 ggcgtatttc actgcaaaat caatgtattg tgcgtgaccc aacgcgattt tatcgtagtc
    32101 ggaatactgc aatgcgatct catcaccgcc gaggtgtgaa aagacataat cgcggccata
    32161 ttcccacgca ctggtggtgt tgaagatatt ggcaagtttg tttaccaggg tggatttacc
    32221 gctcgactcg ccaccaagga tcgccacggt acgcacaaaa aacggcttca cttcggtagg
    32281 aatatattcc cagtagcgga acgggttttc gcggatctgc gcaccgctga tactcataaa
    32341 ggtacgtttc ggatcgacca gcaccgtctc gatccccaga tgttccatat actgtggcgc
    32401 atcggcttct tccgaggtgt agatcaggtc cggctggatc cccttttcag ccataaactt
    32461 tttgatgccg ttgctccaca catcccagcc atgcggatac ggctccatgc cctcttcgtt
    32521 gaaagcatga atacggatat ttttctgata tttaaaggtt tgcaatagcc agcgcagacg
    32581 atccggcacc gtgggctgct gcgacatggc gctgtcttca aacaacgcgc ggtcgcgggt
    32641 atcgtcaaaa cccataatga tatgcagctc gtcaacctgg ctacaggcgc gctggataag
    32701 gtagatatgt ccggtatgca gcgggtagaa cttaccgaat acgacaccaa tggttttctt
    32761 ctgccgggga aattcaagcc ccaaaaaacg gtgcaatgcc tccagctttt gcgcgctggg
    32821 gcttttaatt ttggcattca gtaactggct taaataccct ttggtcatac cgctggcatc
    32881 agctacctgc tgtagcgtgc agccctgttg cttgatggca gttttcaggt aatcaaatga
    32941 cgacatatct ccctccgtat ctctcattat aagtcgtcga acacgctaag cgcgtcggag
    33001 agttttttaa cgccaaaaat ctgcatccct tccggcgctt ttttcggtac gttagccgcc
    33061 ggaacaatcg cccggcgaaa accgtgtttc gccgcttcag aaatccgctc ctggccactg
    33121 ggcaccgggc ggatctcccc tgccagcccg acttcaccaa acaccaccag atcctgtggc
    33181 agcggtctgt cacgcaggct ggaaaccatc gccagcagta acgctaagtc ggcgctggtt
    33241 tcggttacct tcacgccgcc gaccacgttc acaaacacat cctgatcggc catttgcaga
    33301 ccaccgtgac ggtgcaagac agccagcagg attgccagac ggttttgttc cagccccact
    33361 gccacgcggc gcggattcgc cattatcgag tgatcgacca gcgcctgaat ctccaccagc
    33421 aacggacgcg ttccttccca caccaccatc acggagctgc cggaggtcac ttcatctccg
    33481 cgacttaaga aaattgccga agggttgctg acttcacgca gcccctgttc ggtcatcgcg
    33541 aagacgccca gctcattcac cgcgccgaag cggtttttat ggctgcgtaa ggtgcgaaaa
    33601 cgggagtcgg catcgccatc cagaagcacc gaacagtcga tacagtgttc cagcactttc
    33661 gggccagcca gcgaaccatc tttggttacg tgtccaacca tgacaatcgc cacaccgcgc
    33721 gttttggcga aacgcgtcag ataagccgcc gtttcacgca cctgcgccac gctaccaggc
    33781 gatgattgta catccgccat atgcatcacc tggatcgagt caattaccat cagcttcggt
    33841 tgctcttctt cggcaatcag gcagatctgc tcgatgctgg tttccgacaa catattgaga
    33901 ttgtcagtcg gcaggccaag gcgatgagcg cgcattgcca cctgttgcag cgactcttcg
    33961 ccggtgacat acagcgtttt catctgctgg gccagtttgc acagcgtttg cagcaacagc
    34021 gtggatttcc ccgcaccagg gttaccgcca atcagaatgg cacttcctgg caccacgccg
    34081 cctcctagta cgcggtcgaa ctctttaaat ccggtggaaa aacgcggcag ctcttcaagg
    34141 ctgatatcgg agagtttctg gacttttgcc accccggcgc taccggcata gccgctgaga
    34201 cgctcgttac gcgccaccgt tggcgacgca gcaagacgca cctcggtgat ggtgttccag
    34261 gcatgacagg cactgcactg cccctgccag cgcggataat cggccccgca ttcattacaa
    34321 acaaaggcgc gttttggagc ttttgccacg gtgtacctcg ttaatgctgt gccgcccgca
    34381 ggatgacggg caggcaatta cttctgattc aggctgccag agaggatgca gaataccccc
    34441 atcaggtcag cgtgacggat ggtgacttcc gccttttcat tcacttttgg cttggcatgg
    34501 taggcaatcc ccagccctgc tgctttgatc atcggcaggt cattagctcc atcgccaatc
    34561 gccacggtct gcgccagcgg gatttcatac tcctgcgcga ggcgagtcag agttttcgct
    34621 ttgtactgcg cgtctacgat gtcgccgatc acattgccgg taaatttacc gtccatgatc
    34681 tccagttcat tggctaccac ggctgtcagg cgcagcttgt cgcgcaggta ttcagcaaag
    34741 aaagtaaagc cgccggaggc aatcgccact ttccagccca gcgtttccag cttgagcacc
    34801 agctgcgtta agcctggcat cagcggcaga ttttcacgca cctgttgcag aatattggcg
    34861 tcagcgcctt tcagcgtcgc cacgcggctg cgcaggctgg cggtaaaatc gagttcgccg
    34921 cgcatcgccc gttcggttac ctccgccacc atctcgcccg ttccggccag cttggcaatt
    34981 tcatcaatac attcaatctg gatggcggtg gaatccatgt ccatcaccag caaacccggc
    35041 gtgcgcaggt gcgggatttt ccccagcggg gcgacatcca gctgcgcttc gtgggccagg
    35101 cgtgtagccc gtgcggtgag tgaacctgcc agacgaatca cctgataatc ttccacgcac
    35161 caggcggcaa caatcaccat cgccgcaccc agtttgctct ggtattgggt cagacgctgt
    35221 ttatccagcc cacgaccata cagcagccag ccgctacgac ctgcgtggta atccagtggc
    35281 atcacttcat caccacttaa tgaaagaggc agacccggcc ataaagagac atcttcaggc
    35341 aggtcgcacc aggtaatgtt aggcattaag gctcctgtaa aatcgttcga agcagggaaa
    35401 ataacgcatg aggctacctt gtatccattg cttctggcaa cattaagtct caaattttca
    35461 aagggtggaa gatggctcgc acaaaactga aattccggct acatcgggca gtgattgtcc
    35521 tgttctgtct tgccttgtta gtggcactga tgcagggggc gtcatggttt agtcaaaacc
    35581 accagcgaca gcgtaatcca cagctggaag aactggcccg caccctggcg cgtcaggtga
    35641 cgctgaacgt tgcaccgctg atgcgtaccg actcaccgga tgaaaaacgc attcaggcga
    35701 tcctcgatca gttaacagat gaaagccgta tcctcgacgc gggtgtgtat gacgaacaag
    35761 gcgatcttat cgcacgttct ggcgaaagcg tcgaagtgcg cgatcggctg gcgctcgacg
    35821 gtaaaaaagc aggcggctat tttaaccagc agattgtcga gccaattgcg ggtaaaaacg
    35881 gaccgctcgg ctatctgcgc ctgacactcg acacccatac gctcgccacc gaagcccaac
    35941 aggtggataa caccactaac attttacgcc tgatgttgct gctctcactg gcaatcggcg
    36001 tagtgctgac ccgcacgctg ctacagggta aacgcacccg ctggcagcaa tcgcccttcc
    36061 tgttaaccgc cagcaaaccg gtgccggaag aggaagaaag cgagaaaaaa gagtgaccca
    36121 ttactacaag aaaggaaatc gttatgtcca cattacgcct gctcatctct gactcttacg
    36181 acccgtggtt taacctggcg gtggaagagt gtatttttcg ccaaatgccc gccacgcagc
    36241 gcgttctgtt tctctggcgc aatgccgaca cggtagtaat tggtcgcgcg cagaacccgt
    36301 ggaaagagtg taatacccgg cggatggaag aagataacgt ccgcctggcg cggcgcagtg
    36361 gcggtggcgg cgcggtgttc cacgatctcg gcaatacctg ctttaccttt atggctggca
    36421 agccggagta cgataaaact atctccacgt cgattgtgct caatgcgctg aacgcgctcg
    36481 gcgtcagcgc cgaagcgtcc ggacgtaacg atctggtggt gaaaaccgtc gaaggcgacc
    36541 gcaaagtctc aggctcggcc tatcgcgaaa ccaaagatcg cggcttccac cacggcacct
    36601 tgctactcaa tgccgacctc agccgcctgg caaactatct caatccggat aaaaagaaac
    36661 tggcggcgaa aggcattact tcagtgcgtt cccgcgtgac caacctcacc gagctgctgc
    36721 cggggctcac catgagcagg tttgcgaggc cataaccgag gcctttttcg cccattatgg
    36781 cgagcacgtg gaagcggaaa tcatctcccc ggacaaaacg ccagacttgc caaacttcgc
    36841 cgaaaccttt gcccgtcaga gtagctgaga gtggaacttc ggtcaggctc cggcattctc
    36901 gcatctgctg gatgaacgct ttagctgggg cggcgtggaa ctgcatttcg acgttgaaaa
    36961 aggccatatc acccgcgccc aggtatttac cgacagcctc aaccccgcgc cgctggaagc
    37021 cctcgccggg cgactgcaag gctgcctgta ccgcgcggat atgctgcaac aagagtgcga
    37081 agcgctgttg gttgacttcc cggaccagga aaaagagcta cgggagttgt cgacgtggat
    37141 agcgggggcg gtaaggtaac tcgctgatta aaatatccaa ggaaggaaat atgatcgatc
    37201 tggaaaacca ggaacgggag attatcaatt taatgttcag ccagcgtatt tcctggctag
    37261 ccgccgtgcg tatcaggcat aaattatcgc ttgctgaagt aagtaaaatg ttaggtatct
    37321 cgattaattc tctaaaacag atcgaaaaga cggaacggct tagctcaaac atcaaaagca
    37381 aaatggcaga aatttatggt tgtccaccag agttgctgat atgcccttcc tggatgacgg
    37441 ctgaacataa atgaatagat tcgtgaatag atacttgaat gttttttatt ttctatccat
    37501 atgaaaaata atagtaaatc tataaatcag ttgctattga tgaatagata ctaaaaagcc
    37561 ggagcagtcc cccactccgg cttaacacga caatcgcctt tcagcgaaac acaattactc
    37621 tttatcgccc agcagaacgg attccagtgc gattttgatc atgtcgttga aggtggtctg
    37681 acgctcagcg gcagtggtct gctcgtgagt gcggatgtgg tcagacacgg tgcagatggt
    37741 cagggctttc gcgccaaact ctgcagccac gccgtagata ccagccgctt ccatttccac
    37801 gccgaggatg ccgtattttt ccatcacgtc gaacatttcg ccgtccggag agtagaacag
    37861 gtcagcggag aacaggttac ccacgcgagc atcaacgccc agtgctttag ctgcatctac
    37921 tgcgttacgc accatgtcaa agtcagcgat agcggcaaag tcatggtctt taaaacggat
    37981 gcggttaact ttggaatcgg tgcaggcccc cataccgata acgacgtcgc gcagttttac
    38041 gtgcggcaga actgcgccac aggaaccaac gcggataatt ttcttcacgc cgaaatcggt
    38101 gatcagttct ttggtgtaga tggagcagga cgggataccc ataccgtgac ccattacgga
    38161 aattttgcgg cctttgtaag taccagtgaa gcccagcata ccgcgaacgt tgttcacttc
    38221 acgggcatct tcaaggaaag tttcagcaat atacttcgca cgcagcgggt cgcctggcat
    38281 caaaactacg tcagcgaaat cgcccatttc tgcattaatg tgtggggtag ccattgtttt
    38341 atcctttttg tgacataaca aaggcggagt caacgctccg cccaaatcca tcagaacatg
    38401 gctttgccat attccatatc agaagtacca aaatattttg ccagagtctg gccgatatcc
    38461 gcgaaggttt cacggtgacc cagtgagccc ggttttactt tcgggccata taccagtacc
    38521 ggaatgtgtt cacgcgtgtg gtcagtaccg gtccaggtcg gatcgcaacc gtggtcagcg
    38581 gtgaggatca ggatgtcgtc atcgcgcagc agagacatca gctccggcag acggcggtcg
    38641 aacagttcca gacccgcagc ataaccggcg acgtcgcggc ggtggcccca ggaagagtcg
    38701 aagtcaacga agttggtgaa gacgattgtg ttatcacccg cttctttcat ctctttgatg
    38761 gtggcgtcaa acagcgcgtc caagccagtc gctttcactt ttttggtgat accgcagttg
    38821 gcgtagatgt ctgcaatttt accgacagaa accacctggc cgtgtttttc atcaaccagt
    38881 ttctgcagca cggtcggtgc tggcggctca acagccaagt cgtgacggtt accggtacgc
    38941 tggaagttac cagctttgtc accgataaac ggacgagcga taacacgacc gatattgtag
    39001 ccgccgttgg tcagctcttc acgggcgatt tcgcacagtt cgtagagttt atccagaccg
    39061 aaagtttctt catggcaggc aatctggaac acggagtcag cggaggtata gaaaatcggc
    39121 ttgccggttt tcatgtgctc ttcgcccagt tgatccagaa tgaccgtacc ggaagagtgg
    39181 cagttaccga ggtaacccgg cagattagcg cgttcgacca gtttatccag cagctcttgc
    39241 gggaagctgt tttcgtgatc ggagaaatat ccccactcaa acagaaccgg gacaccggcg
    39301 atttcccagt gaccagacgg ggtatcttta ccggatgaca tttcgtgcgc ccatgcgtac
    39361 gcgccgataa cttcagcgtt gccgtccatt cccgccggaa tgaaaccggt agaaccttcg
    39421 tgtgctttcg ccagccccag acgggtcaga tttggcagat tgagcggccc tttacgacca
    39481 ttatcagctt cgcctttggc acaagcttct gcgatatgac ccagggtgtc agccccgacg
    39541 tcaccaaagc gttctgcatc ttctgtagcg ccgatgccga atgagtccag caccataata
    39601 aatgcacgtt tcatatgttc tccgtactca gtgcttcaaa tttaaatgat cagatcagta
    39661 taccgttatt cgctgatacg gcgatagaca gttggtgtgc tttccggtgc gttatcggca
    39721 agtttaattg ccgctttcac cgctttcgcc gcttcctgcc agctgttttc gtctttcgcg
    39781 aggataaccg ccagcggacg ctgaccgtct acctggtcgc ccagacgcgc catatcagta
    39841 aagccgacgc tgtaatcgat ggtgtcagat gcctgacgac gaccgccgcc cattgcaact
    39901 actgccatcc ccagcgcgcg ggtatccatt tcactgacaa aaccttcggt atcagcatag
    39961 actgctttcg tcagcatcgc tgtcggcaga tacttcgcgt agttctcaac gaagtcggtc
    40021 gggccttttt gtgccgctac catacgacca aagacttctg ccgctttacc gttgtccagc
    40081 accgcctgca atttcgcgcg cgcttcggcg tcatctttcg ccagtttgcc ggagatcaac
    40141 atctccacgc acagcgccat cgtgacatca aacagacgcg ggttacgata ctcacccgtc
    40201 aggaactgca ccgcttcacg aacttcaacc gcgttacctg cactggaggc cagtacctga
    40261 ttcatgtcgg tgagcagcgc ggtggtgcgc acgccagcgc cgttagccac gccaacaatc
    40321 gcttcggcaa gggcttcaga gagttcgtag gtcggcataa acgcgccgct acccactttc
    40381 acgtccatca ccagcgcgtc cagaccttcc gccagtttct tcgccaggat cgaggcagtg
    40441 atcagcggga tggagtccac ggttgcggta atatcacggg tcgcgtagaa acgtttatca
    40501 gccggagcca gtgaactggt ctgaccgata atcgccacgc cgacgtcttt aataatttcg
    40561 cggaaacggt tgtcatccgg gaaaatgtcg aagccaggga tggattccag tttgtcgagc
    40621 gtaccgccag tatgaccgag gccgcgaccg gagatcatcg gaatatagcc gccgcaggct
    40681 gcgaccatcg gccccaacat cagcgaagtg acatcgccga cgccgccggt cgagtgttta
    40741 tcaacaatcg ggccattcag atgcaggctt ttccagtcga gaacggttcc tgaatctcgc
    40801 atcgccatgg tcagcgagac acgctcaggc attgtcatat cgtggaagaa aatggtcatc
    40861 gcgagggcgg caatctgccc ttcggaaata gtgttgtcgc gaataccgtt gataaagaaa
    40921 cgaatttctt catcgctcag cgcatgacca tcacgttttt tacgaataat ttcttgtgcg
    40981 agaaacaagg taacctcccg aggaaaagag tatgaggcgg cttttgccgg atgcgtctac
    41041 gccttatccg gcctacgggt agatacgtct cgtaggcctg ataagcgcag cgcatcaggc
    41101 gtaaagcatc ttacttagta gctgctggcg ctcttaccgt cgccgtggcc cagcgctttc
    41161 agcaggcttg ccagcaggct ggaagcacca aagcggtagt gacgcgcatc agcccagtca
    41221 gcaccgaaca gttcatctgc gatggcgaga tatttctgcg catcttccgc agtacgcacg
    41281 ccgcccgccg gtttgaaacc aacggttttt tctacgccca tatcacggat cacttccatc
    41341 atgatgcgag cgctttccgg cgtcgcgttc acagccactt taccggtaga ggttttgatg
    41401 aagtccgcac ccgctttgat ggagatttca gacgctttac ggatcagcgc ttcgtctttc
    41461 agttcgccgg tttcgatgat cactttcagc agtacattcg ctgccgcgca agcctcttta
    41521 caggctttca ccaggtcaaa accaacctgc tcgtcacccg ccatcagcgc gcggtacggg
    41581 aacaccacgt caacttcatc agcaccgtag gcgattgccg cacgggtttc tgccagcgcg
    41641 atttcgatgt cgtcgttacc gtgtgggaag ttggttaccg tagcgatacg gatttccggg
    41701 gtgccctgct ctttcagagt tttgcgagca atcgggataa aacgaggata gatacagata
    41761 gcggcggtat tgccgaccgg agttttggcc tgatgacaca gagcaattac tttctcgtcg
    41821 gtgtcgtcgt cattcagggt ggtcaggtcc atcaatttca gtgcacgcag gctgcttgct
    41881 ttcagatcag tcatttcatt ctcctggctt gtcgccaata aaattcacct tgcgagtttg
    41941 ttagtattct aacatctact ccgcaacaca cttcgataca catcacaatt aaggaaatct
    42001 acttacaagt ttgcatcact gtaatgcgat ctggttcaaa taattcactt tcaaatgaat
    42061 gcgtcagtgg tggcaaacgc atcaggatca aagtgaacat cacgaaactt cttacaatgg
    42121 cgcatcacct tcggcataaa aggaaaagaa gatgcccact tctcatgaaa atgcactgca
    42181 acaacgttgc cagcaaattg tcaccagccc agtgcttagc ccggagcaga agcgccattt
    42241 tctggcactg gaagcagaaa acaatctgcc ttacccacag cttcctgccg aagcccgccg
    42301 cgcgctggat gaaggtgtaa tctgcgatat gtttgaaggt catgcgccgt acaaaccgcg
    42361 ctatgtctta cccgattacg ctcgttttct ggcgaacggt tccgaatggc tggagctgga
    42421 aggcgcgaaa gatcttgatg acgcactctc tctgctgacc attctttacc accacgtacc
    42481 gtcggtcaca tcgatgccgg tctacctggg gcaactggat gcgttgttgc aaccgtatgt
    42541 tagaattcta acacaaaacg agatcgatgt tcgaataaaa cgtttctggc gttacctcga
    42601 cagaaccctg ccagatgcct ttatgcacgc caatatcggc ccgtctgatt cgcccattac
    42661 ccgcgcgatc ttacgtgcag acgcgcaggt ttcaccgaac ctgaccttta tctacgatcc
    42721 tgaaatcacc cctgatgacc tgctgctgga agtggcgaag aacatctgtg aatgtagcaa
    42781 accgcacatc gccaacggtc cggtgcatga taaaattttc acaaaagggg gctacgggat
    42841 tgtgagctgt tacaactcac tgccgctggc gggtggtggc agcacgctgg tacgccttaa
    42901 cctgaaagcc attgccgagc gcagcgaatc gctggatgac ttctttacgc gcactctacc
    42961 gcactactgc cagcagcaga tcgccatcat cgatgcgcgg tgtgaattcc tctatcaaca
    43021 atcacacttc tttgagaata gcttcctggt gaaagaaggg ctgattaacc ctgaacgttt
    43081 tgtgccaatg tttggcatgt atgggctggc ggaagcggtt aacttgctgt gtgagaaaga
    43141 agggattgcc gcgcgctacg gtaaagaagc cgccgcaaat gaagtgggtt atcgcatcag
    43201 cgcgcaactg gcggagtttg tcgccaatac ccccgtgaaa tatggctggc aaaaacgcgc
    43261 catgttacac gcacagtcgg ggatcagttc cgatatcggc accacgccgg gcgcgcgttt
    43321 gccgtatggc gatgagccag atccgatcac ccatctgcaa actgtcgccc cgcatcatgc
    43381 ttattattat tccggcatca gcgacattct gacgctcgac gaaaccatca aacgtaaccc
    43441 gcaggcactg gtacagcttt gcctcggtgc ctttaaagcc ggaatgcgtg aatttaccgc
    43501 caatgtcagc ggtaacgatc tggttcgcgt taccggttat atggtgcgtt tgtcggattt
    43561 agaaaaatat cgcgccgaag gttcacgcac caacaccacc tggctgggcg aagaagccgc
    43621 acgcaacact cgtattctgg aacgccagcc gcgcgtgata agccatgaac agcagatgcg
    43681 ctttagtcag taagattatc cccttctcct gcgttgacgg gccaggcagt cgtctggctc
    43741 tgtttttgca gggctgcaat ctgcgctgca aaaactgtca caatccgtgg acgatgggac
    43801 gttgcaatga ctgtggggag tgcgtgccac agtgtccgca tcaggcgttg cagattgttg
    43861 acggcaaagt ggtgtggaac gctgtggtct gcgagcaatg tgatacctgc ctgaagatgt
    43921 gtccgcaaca tgccacacca atggcgcaat ccatgagcgt ggacgaagtg cttagccatg
    43981 tgcgcaaagc ggtgctgttt attgaaggga taacggtaag cggcggcgaa gccacgaccc
    44041 aactgccgtt tgtagtggcg ctgtttactg ctatcaaaaa cgatccgcaa ctgcgccatc
    44101 tcacctgtct ggtggacagt aacggtatgt tgagcgaaac cggctgggaa aaattactcc
    44161 cggtgtgtga cggcgcaatg ctcgatctca aagcatgggg tagcgaatgt catcaacaat
    44221 taaccggacg cgataatcaa cagattaagc gcagcatcta tttgctggca gagcgcggca
    44281 agctggcgga actgcgtttg ctgatgatcc caggccaggt ggattatttg caacacatcg
    44341 aagaactggc ggcgtttatc aagggacttg gtgatgttcc ggttcgcctg aacgcgtttc
    44401 atgcccacgg cgtgtatggc gaggcgcaaa gctgggcgag cgccacgccg gaagacgttg
    44461 agccgttggc tgatgcatta aaggtgcgcg gggtgagccg gttgatattt ccggcgctct
    44521 atttgtgaca tgccggatac ggcctacggt tcggcacaga cttgtaggcc tgataagacg
    44581 cgtcaagcgt cgcatcaggc attgtgcgcc aactgccgga tgcggcgtga acgccttatc
    44641 cggcctacgg ttcggcacaa acctgtaggc atgataagac gcgttaagcg tcgcatcagg
    44701 cattatgcgc cgactgccgg atgctgccta cgaagggcaa actacccacg aacgttaaac
    44761 accgcatacg tgttattaag caacacttcg gcaatctcat ccgcgggttc cgggcgtaac
    44821 tcgcaaagca cggcaaacac acgggcagcc tgttccgggc ggttaggctg cccctgaaaa
    44881 ccgttgagcg gcatatccgg cgcatcggtt tccagcaaca acgatgccag cggtaatttt
    44941 gcgatgacat cgcgggtttt actggcgcgt ggataggtga tagtaccgcc tacgccgatt
    45001 ttgtagccca gctgtacaaa ccgttcagcc tgttgctggc tgccagaaaa tccgtggaca
    45061 acaccagcac gcggcaaatc gtggcgttta agatgcatcg ccagtttgtc gtgcgtgcgc
    45121 cgtgaatgca ggatcaccgg cagatcgtag cgtttcgcca gtttcagttg ttcgtcgagt
    45181 aaccactgct gcctctcaaa ttgcggatcg tcgccgaaga gatccagacc gatctccccc
    45241 accgccacca ccttcgccgg acgcctttcc agcgcctgct gtagctgctc aagagacaca
    45301 tcgctatgtt tttccaacat accaggatgc aagcccaata cggcatacag cggttgataa
    45361 ttttccgcta acgcctgcac acgggcaaaa ttttccgcct ctgtcgccgg aacaatgatc
    45421 ttgcctacgc ccgcttgtgc cgcgcgttgc aggctggcct cttcatcgcc aataaaaggc
    45481 gggaaatcaa aatggcagtg agtgtcgata aaacggcaaa tcaagccaga tcctcattgt
    45541 taaatgtcgg gtcgttcgcc tgcggagcgt cgctcacttc ggcaaccagc gtatcgttgg
    45601 cgactggcgc aggcggaatg acaatcgatt ccggcgtcac cactggcacc agatggcggg
    45661 taagcggcgc tttttcagtt aatagcttgc caaccgtggc gaggaaataa cgaccgcata
    45721 aacgcccaag tttatagtct tcacgcagcg ccggaatccg actgccaagc gcgatactat
    45781 gtaatggctt cggcggataa atttcggata tccgcagctt gcccggtggt ttctcaataa
    45841 attgctgaat gtcacgatag ctggtttcat gatgctgcac cagattgacc agcggctgca
    45901 ggctactgtc acccagccag cgttccatgc gtttgaacca ctgcggcgtg tagtacattt
    45961 gtgacggcac agtgcggatg acgaccaacg ttttagcgcc ctgccttgcc gcctctttaa
    46021 ccggaatcgc atcactgatc ccgccatcca ggtagttaat gccttccagc gacactccgc
    46081 tacgataaaa gccaggtatc gcactggagg cgcgaatcac atccagccag ttttgtttgg
    46141 ttggtaaaaa gtaattcggc gcgtagtcat cctgacgaca ggcgcacata taaaacgatt
    46201 tgccgctgtc aaacaaccgc gcggcggtat ccatttgcaa cggcatctgg cttgcggtgg
    46261 cctccaccag ccagtcgaga tcgataagat ttcctccacg gacaaagcgc aatggatcga
    46321 aaaattcgcg ttttgtggta tagcgcatga tgactttgcg cgcgtaaccg ggctgattgc
    46381 agataaacgc cgagaggttc tgcgccccgg cagatgtgcc gagataaaga tcgaaaggat
    46441 taaactgcgc gcgcataaac tcatccagca cgccagccgt gaaaattcca cgctgtccgc
    46501 cgccttcgca caccagagct attcgtccag gctggaacgg ccttagcgac aacggcgcaa
    46561 tattaccaag cgttacaggt attcgctgcc ccacttctgc cttcctgttt ttgttgtgaa
    46621 agaaagacaa agtaacgcaa tttattattc gctaaaaccg caaaagccag tccgctggac
    46681 tggctttgga taaaattttc ttaatggtct gaaatctagg gtcgttttcg gcccatgaac
    46741 aaactcacta ggaacagaat aatcccgacg acaaagacaa ttttagctgc gcctgcagcg
    46801 gtaccggcca gaccaccaaa cccaagtgcg gcggcgatta acgcgataac cagaaatatg
    46861 atgccccaac gaaacataag cgtctccttt accatagtta atgtcaccgc ttgatatgag
    46921 cgtgaaaatc actcaatgcg atatttttag tgtggtgcac atcgcgcctc ccgacaaagt
    46981 tcgggaggac gaattactta gttttcagat catttttaac gcttttcaca ccatctaccg
    47041 ctttggcgat actttcagca cggtcacttt gtgcctgaga atctacggta ccggagagct
    47101 gaaccacgcc atcggtggtt tcaactttca ctttacgtga agggacgata tcgtccgcca
    47161 gcagtttggc tttgatttca ctggtggtgg cggtgtcacc cgcgtagccc ttcaccgagc
    47221 cttctttagc gtcgcgaacg tgcagtttgt cgctgacaga ggtcacgcct tcaacgcctt
    47281 tcgccacttt cactgcctct tcggcctggg cctgactttc aacgaaaccg ctcagggtca
    47341 cgactttttg atcggttttg acagagatat cggtgctctt gatgttgtca tgatccacca
    47401 gcgccgcctt cactttcgcg gtgatggcgc tgtcatccat gaaattaccg actttattca
    47461 tagagctatc gactttttgc cctgcgcttt cattggtagt ctgcgcgttg ttttccgcgt
    47521 aggcagagcc ggtcgcgacg gcagaggtca acattacagc cagcagagtt ttcgaaatct
    47581 tcagtcttgt catagtcatc gatttattcc tgtatgtttg ctcgtaattt gagcctagca
    47641 acacgaggtt gcattgctga atagggagag acttcaccct ctacagaagt caatggtcgc
    47701 catcacaaaa gcgatgggtg atgaataacg accattacag cctctgaatc agttattaat
    47761 atcggtagaa tgacaatcga cggctttaga tactaatatc tacgcattga acggtattta
    47821 acgccatcag aaatgtcatc actttgttaa atatagatca caattttgaa accgctcggg
    47881 atatcagcga aaacataagc aaaagtgaat gttttaagaa cattccgtaa gcggctaata
    47941 aggaagggaa attgacaggg cgcagcggct accgcgccct ggaggcaaga attaatgctc
    48001 gcgggtctgg tggaactgaa cgtccggata acgttcctgt gccaggcgca ggttgaccat
    48061 gctggtagcg atgtaagcga ggttatcgcc gccatcaagc gccagttggc tttcgttctt
    48121 acgcttgaac tcttcgaatt tcttcgcgtc tgcacattct acccagcggg cagtggcaac
    48181 gttgactgac tcatacactg cttcaacgtt gtattcgctc ttcaggcgcg ctaccaccac
    48241 atcaaactgc agcacaccaa ccgcaccaac gatcagatcg ttgttggaga ttggacggaa
    48301 cacctgcacc gcgccctctt cggaaagctg taccagccct ttgagcagct gtttttgctt
    48361 cagcggatct ttcaggcgga tacgacggaa cagttctggt gcgaagttcg gaataccggt
    48421 gaacttcatc atctcaccct gggtaaaggt gtcgccgatc tgaatggtgc cgtggttgtg
    48481 caggccgagg atatcgcccg gatacgcttc ttcaacgtgc gaacggtcac ccgccataaa
    48541 ggtcagcgcg tcggagatca ccacatcttt cgcagtgcgc acctggcgca gtttcatgcc
    48601 tttttcatat ttaccggaca ccacacgcat aaacgccacg cggtcgcggt gtttcggatc
    48661 catgttggcc tgaattttaa atacgaagcc ggtaaattta tcttcgctcg cttctacggt
    48721 acgggtatcg gtctgacgcg gcatcggcgc aggcgcccac tccaccaggc cattcagcat
    48781 atgatcaacg ccgaagttac ccagcgcagt accgaagaat accggagtga tttcgcccgc
    48841 aaggaacagc tctttgtcga actcgttaga cgcgccttta accagttcca gttcgtcacg
    48901 cagctgctgt gccagatctt caccaaccgc agcatcgaga tccgggttat tcagcccttt
    48961 aacaatgcgg acttcctgaa tggtgtgccc tttaccgctc tgatagagat aggtttcatc
    49021 tttataaagg tggtaaacgc ctttaaacag cttgccgcag ccaattggcc aagtgatcgg
    49081 tgcgcagcca attttcagct cgttctcaac ttcatcgagc agttccatcg ggtcgcggat
    49141 atcacggtca agtttgttca taaaggtgag gatcggcgtg tcgcgcagac gggtaacttc
    49201 catcagctta cgggtacgat cttcaacacc ttttgcggcg tcgataacca tcaggcagca
    49261 gtccaccgcc gtcagggtac gataggtatc ttccgagaag tcttcgtgcc ccggggtgtc
    49321 gagcaggtta accaggcaat cgtgatacgg aaactgcatc acagacgtag taatggagat
    49381 cccacgctgc ttttccatct ccatccagtc cgacttagcg tgctggttgg aaccacggcc
    49441 ttttactgta ccggcggtct gaatggcctg tccgaacagc agcaccttct cggtgatggt
    49501 agttttaccg gcgtccgggt gagaaataat ggcaaaagtg cggcgcttcg ccacctcttt
    49561 caaataagga gacaacgtca taattctttc ttcttgagta agcggcatcg cgccgcgctt
    49621 gttgaaaacg aaaaattgcg gctattttac ccatcaacgg gggggaggca atcagtgttt
    49681 acacaggagc tgctccagtt cgtgcaacga agaaacggtc caggtgggcg cgatgccttc
    49741 tggttgctcg cgattgtgcg cattcagcca gcaggtcgca agcccggcgt tgatgccacc
    49801 gagaatatcg gactcggcag tgtcgccaac catcagcacg cgtgaacggt caggattgcc
    49861 cgcctgttcc agcgcataat cgaaaatttt cttattcggt ttggcaacgc caacttcttc
    49921 ggaaatcacc agcaaatcga agtaatcacg caggcccgtc cgttccagac gcacttgctg
    49981 caaggcgcta aaaccgttgg tgatgatgcc gattttagcg ttgctacgaa tggcgttaag
    50041 cagagaaacc gcgcccggca acggcgtaca gatttccgcc atcgcattaa taaaggcatc
    50101 gttaagcaag cctgcttcaa ctttcagccg ttcggcccag ctctcgaaac gcccgtgctg
    50161 aagctgtaat gaagtgatcg cgccgttttg ataatccacc cacagtggct tgttaacggc
    50221 ctgatagtcc tgaaaatctt cagcggtaaa ggtgacgctg taatcaagaa acatccgctg
    50281 caggccggtg aatgagtcaa aggtaaacag cgtttcatcg gcatcaaaga aaatccagtc
    50341 ccacttcatt attccacctt gtcttacata ctgattggca acgccatgat gatggcgtct
    50401 tcgcgaccgt ccgtggtggg gtagtaattg cggcgaatcg tcgcctcgtt aaagcctaaa
    50461 ctttcgtaca gggcaatggc ggcagcattt gaggcacgga cttccagcca tagtgtcgcc
    50521 acgccgcgtt tttccagttc gtcgatcaga tgttccagca gcgccctacc cagcccctga
    50581 cgctgatagt cgggatcgac cgcaatattg aacaatgtag cttcatccag caccacttgc
    50641 gtaatcgcaa acgccgccat tttgccgttt tgcgttaact gaaagttgag ataacgctcg
    50701 ccctggttgc tggcaaaggt tttttcactc cacggaaagg cgtgggcgcg ttgttcaatg
    50761 tggtaagccg ctggtaaatc agtcgtttcg agggaagaaa tcgtgttcat atgtgcaaat
    50821 ttgttgccat aacgcggcgc gtgccgttgg gtttgcccgt aattcggtga gcgccggtga
    50881 tgccacctga gcgccttcca atgatagcgg ttcgtcagta cccaaccgcc agctgttgca
    50941 gcgactgcct tgcggcagca tcgcgatttt ttctggcgtc agttgcagca cttgatcggg
    51001 gctgacggtt aatgcgcgca gaacatcgct cactaaagga tcagtcaggg cgggaagatc
    51061 gtttgccacc atcaccagac ggacgtgtgc cgggctcgca atggcaatct ccccctgcaa
    51121 cgcgccaggg cgacgcagcg accactgggt aatgcccagt tgctgtaact gccagtctcg
    51181 tcgggatgtc atagcgaatc gctcctgttg tcaggggcgc aaatatagca aattcgtcga
    51241 taccgcgcca acatatggct ataatcgccg ccagtatcaa ttgaggagca ttccatgtct
    51301 gcatttaccc cggcaagtga agtcttgctg cgtcacagtg atgatttcga acaaagccgt
    51361 attctgtttg ccggagactt acaggatgac ctgcccgcgc gtttagatac cgcggccagc
    51421 cgtgctcata cccagcaatt ccaccattgg caggtgttaa gctgccagat gggggataac
    51481 gcccgtttta gtctggtcgc cacggcgaat gatgtcgcag attgcgatac gctgatttac
    51541 tactggccga agaacaaacc ggaagcccag ttccagttga tgaatttact ttctctgctg
    51601 ccagtgggga cagatatttt tgtcgttggc gagaaccgca gcggcgtgcg cagcgctgag
    51661 cagatgctgg cagattatgc gccgttgaat aaagtcgaca gcgctcgtcg ctgtggcctc
    51721 tattttggtc gtctggaaaa acagccagta tttgatgcgg ataaattctg gggcgaatac
    51781 agcgtcgatg gcctgacggt caaaacgctg cctggcgtgt ttagccgcga cggtctggat
    51841 gtcggtagcc agttgctgct ctcgacgtta actccgcaca cgaaaggtaa agtgctggat
    51901 gtcggctgtg gcgcgggtgt actttcagtt gcctttgcgc gccattcgcc gaaaattcgt
    51961 ctcaccttgt gcgatgtctc tgccccggcg gtagaagcca gccgcgcaac acttgcagcc
    52021 aacggtgttg aaggtgaagt ctttgccagc aacgtctttt ccgaggtgaa aggttgtttt
    52081 gatatgatca tctccaaccc gccgttccac gatgggatgc aaaccagcct ggatgcggcg
    52141 caaacgctga ttcgtggcgc ggtgcgtcat cttaatagcg gcggcgagct gcgaattgta
    52201 gcgaacgcct tcctgcctta cccggacgtg ctggatgaga catttggctt ccatgaagtt
    52261 atcgcgcaaa ccgggcgctt caaggtatat cgcgccatta tgacccgcca ggcgaagaaa
    52321 ggttgagtat ctgatatctg tcgccgcata ccgtgtcgga tgcggcgtga acgccttatc
    52381 cgacctacaa aacctgcacg ttagcccttc gtaggccaga taagacgcgc cagcgtcgca
    52441 tctggcatct gcccccgcat gcctaccaaa cctgcacgct agtttcctga tggacatttt
    52501 tccagcaatt acacatcagg cgataattaa ctattgacga aaagctgaaa accactagaa
    52561 tgcgcctccg tggtagcaat tctttttaag aattgatgg
//
LOCUS       DAJTSA010000019        49828 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000019 DAJTSA010000000
VERSION     DAJTSA010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49828
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.019"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1222
                     /locus_tag="OO369_001179"
     CDS             <1..1222
                     /locus_tag="OO369_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5981528.1"
                     /translation="ELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENHP
                     VITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMII
                     PEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNIA
                     LLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVYV
                     NTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKNF
                     KVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPETV
                     LNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEKY
                     LLAQFGVDIGEAAQ"
     gene            1219..2184
                     /gene="yggF"
                     /locus_tag="OO369_001180"
     CDS             1219..2184
                     /gene="yggF"
                     /locus_tag="OO369_001180"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5981529.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2206..2715
                     /gene="fumE"
                     /locus_tag="OO369_001181"
     CDS             2206..2715
                     /gene="fumE"
                     /locus_tag="OO369_001181"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR5981530.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2712..3425
                     /locus_tag="OO369_001182"
     CDS             2712..3425
                     /locus_tag="OO369_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR5981531.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3397..4074)
                     /locus_tag="OO369_001183"
     CDS             complement(3397..4074)
                     /locus_tag="OO369_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5981532.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4068..4745)
                     /locus_tag="OO369_001184"
     CDS             complement(4068..4745)
                     /locus_tag="OO369_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR5981533.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4733..5116)
                     /locus_tag="OO369_001185"
     CDS             complement(4733..5116)
                     /locus_tag="OO369_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR5981534.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5132..5440)
                     /locus_tag="OO369_001186"
     CDS             complement(5132..5440)
                     /locus_tag="OO369_001186"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981535.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5441..6019)
                     /locus_tag="OO369_001187"
     CDS             complement(5441..6019)
                     /locus_tag="OO369_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR5981536.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6042..6473)
                     /locus_tag="OO369_001188"
                     /pseudo
     CDS             complement(6042..6473)
                     /locus_tag="OO369_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6845..7864
                     /gene="epd"
                     /locus_tag="OO369_001189"
     CDS             6845..7864
                     /gene="epd"
                     /locus_tag="OO369_001189"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR5981537.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7914..9077
                     /gene="pgk"
                     /locus_tag="OO369_001190"
     CDS             7914..9077
                     /gene="pgk"
                     /locus_tag="OO369_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR5981538.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9292..10371
                     /gene="fbaA"
                     /locus_tag="OO369_001191"
     CDS             9292..10371
                     /gene="fbaA"
                     /locus_tag="OO369_001191"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR5981539.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10562..11422
                     /gene="mscS"
                     /locus_tag="OO369_001192"
     CDS             10562..11422
                     /gene="mscS"
                     /locus_tag="OO369_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR5981540.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11561..12196
                     /gene="argO"
                     /locus_tag="OO369_001193"
     CDS             11561..12196
                     /gene="argO"
                     /locus_tag="OO369_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR5981541.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12289..13029
                     /locus_tag="OO369_001194"
     CDS             12289..13029
                     /locus_tag="OO369_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR5981542.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13196..14146
                     /locus_tag="OO369_001195"
     CDS             13196..14146
                     /locus_tag="OO369_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5981543.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14088..15566)
                     /gene="scpC"
                     /locus_tag="OO369_001196"
                     /pseudo
     CDS             complement(14088..15566)
                     /gene="scpC"
                     /locus_tag="OO369_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15590..16375)
                     /gene="scpB"
                     /locus_tag="OO369_001197"
     CDS             complement(15590..16375)
                     /gene="scpB"
                     /locus_tag="OO369_001197"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR5981544.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16386..17381)
                     /gene="meaB"
                     /locus_tag="OO369_001198"
     CDS             complement(16386..17381)
                     /gene="meaB"
                     /locus_tag="OO369_001198"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR5981545.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17374..19518)
                     /gene="scpA"
                     /locus_tag="OO369_001199"
     CDS             complement(17374..19518)
                     /gene="scpA"
                     /locus_tag="OO369_001199"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR5981546.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19722..20615)
                     /gene="argP"
                     /locus_tag="OO369_001200"
     CDS             complement(19722..20615)
                     /gene="argP"
                     /locus_tag="OO369_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR5981547.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            20998..21657
                     /gene="rpiA"
                     /locus_tag="OO369_001201"
     CDS             20998..21657
                     /gene="rpiA"
                     /locus_tag="OO369_001201"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR5981548.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21913..23145
                     /gene="serA"
                     /locus_tag="OO369_001202"
     CDS             21913..23145
                     /gene="serA"
                     /locus_tag="OO369_001202"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR5981549.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23374..23433
                     /gene="ibsC"
                     /locus_tag="OO369_001203"
     CDS             23374..23433
                     /gene="ibsC"
                     /locus_tag="OO369_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR5981550.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23766..23825
                     /locus_tag="OO369_001204"
     CDS             23766..23825
                     /locus_tag="OO369_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5981551.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23926..24528)
                     /locus_tag="OO369_001205"
     CDS             complement(23926..24528)
                     /locus_tag="OO369_001205"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR5981552.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24549..24732)
                     /gene="ssrS"
                     /locus_tag="OO369_001206"
     ncRNA           complement(24549..24732)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO369_001206"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24774..25103)
                     /gene="zapA"
                     /locus_tag="OO369_001207"
     CDS             complement(24774..25103)
                     /gene="zapA"
                     /locus_tag="OO369_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR5981553.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25271..25849
                     /gene="ygfB"
                     /locus_tag="OO369_001208"
     CDS             25271..25849
                     /gene="ygfB"
                     /locus_tag="OO369_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR5981554.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25875..27200
                     /gene="pepP"
                     /locus_tag="OO369_001209"
     CDS             25875..27200
                     /gene="pepP"
                     /locus_tag="OO369_001209"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR5981555.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27197..28375
                     /gene="ubiH"
                     /locus_tag="OO369_001210"
                     /gene_synonym="visB"
     CDS             27197..28375
                     /gene="ubiH"
                     /locus_tag="OO369_001210"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR5981556.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28398..29600
                     /gene="ubiI"
                     /locus_tag="OO369_001211"
                     /gene_synonym="visC"
     CDS             28398..29600
                     /gene="ubiI"
                     /locus_tag="OO369_001211"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR5981557.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29674..29757)
                     /locus_tag="OO369_001212"
     CDS             complement(29674..29757)
                     /locus_tag="OO369_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981558.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30048..31142
                     /gene="gcvT"
                     /locus_tag="OO369_001213"
     CDS             30048..31142
                     /gene="gcvT"
                     /locus_tag="OO369_001213"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR5981559.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31166..31555
                     /gene="gcvH"
                     /locus_tag="OO369_001214"
     CDS             31166..31555
                     /gene="gcvH"
                     /locus_tag="OO369_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR5981560.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31674..34547
                     /gene="gcvP"
                     /locus_tag="OO369_001215"
     CDS             31674..34547
                     /gene="gcvP"
                     /locus_tag="OO369_001215"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR5981561.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34814..35557
                     /locus_tag="OO369_001216"
     CDS             34814..35557
                     /locus_tag="OO369_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5981562.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GTIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35614..37047)
                     /gene="bglA"
                     /locus_tag="OO369_001217"
     CDS             complement(35614..37047)
                     /gene="bglA"
                     /locus_tag="OO369_001217"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR5981563.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37092..37402
                     /locus_tag="OO369_001218"
                     /pseudo
     CDS             37092..37402
                     /locus_tag="OO369_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37566..38225
                     /locus_tag="OO369_001219"
     CDS             37566..38225
                     /locus_tag="OO369_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR5981564.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38421..39401)
                     /gene="ygfZ"
                     /locus_tag="OO369_001220"
     CDS             complement(38421..39401)
                     /gene="ygfZ"
                     /locus_tag="OO369_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR5981565.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39644..39910
                     /gene="sdhE"
                     /locus_tag="OO369_001221"
     CDS             39644..39910
                     /gene="sdhE"
                     /locus_tag="OO369_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR5981566.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39891..40298
                     /locus_tag="OO369_001222"
     CDS             39891..40298
                     /locus_tag="OO369_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR5981567.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40338..40859)
                     /gene="fldB"
                     /locus_tag="OO369_001223"
     CDS             complement(40338..40859)
                     /gene="fldB"
                     /locus_tag="OO369_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR5981568.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40971..41867
                     /gene="xerD"
                     /locus_tag="OO369_001224"
     CDS             40971..41867
                     /gene="xerD"
                     /locus_tag="OO369_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR5981569.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41892..42602
                     /gene="dsbC"
                     /locus_tag="OO369_001225"
     CDS             41892..42602
                     /gene="dsbC"
                     /locus_tag="OO369_001225"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR5981570.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42608..44341
                     /gene="recJ"
                     /locus_tag="OO369_001226"
     CDS             42608..44341
                     /gene="recJ"
                     /locus_tag="OO369_001226"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR5981571.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44432..45530
                     /gene="prfB"
                     /locus_tag="OO369_001227"
     CDS             join(44432..44506,44508..45530)
                     /gene="prfB"
                     /locus_tag="OO369_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR5981572.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45540..47057
                     /gene="lysS"
                     /locus_tag="OO369_001228"
     CDS             45540..47057
                     /gene="lysS"
                     /locus_tag="OO369_001228"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR5981573.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47100..47648)
                     /gene="idi"
                     /locus_tag="OO369_001229"
     CDS             complement(47100..47648)
                     /gene="idi"
                     /locus_tag="OO369_001229"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR5981574.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47703..47774
                     /locus_tag="OO369_001230"
     CDS             47703..47774
                     /locus_tag="OO369_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981575.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47771..47896)
                     /locus_tag="OO369_001231"
     CDS             complement(47771..47896)
                     /locus_tag="OO369_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981576.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47898..49346)
                     /gene="uacT"
                     /locus_tag="OO369_001232"
     CDS             complement(47898..49346)
                     /gene="uacT"
                     /locus_tag="OO369_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR5981577.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        12053 a        12451 c        13404 g        11920 t
ORIGIN      
        1 tgaactgcca gaaattaccg ataatgaact gttagtgagt gtaatttctg acagcgtctg
       61 tttatcgacc tggaaagcgg cgttactcgg tagtgaacat aaacgcgtac ccgacgattt
      121 agaaaatcat ccggtcatta ccgggcatga atgtgccggg gttattgtcg aagtgggtaa
      181 aaatctcact ggcaaatata aaaaaggcca gcgttttgta ttgcaaccgg cgatggggtt
      241 accaagcgga tattcagcgg gctacagcta cgaatatttt ggcggcaatg ccacttatat
      301 gattattccc gaaatcgcca ttaatttggg ctgcgtatta ccgtatcacg gctcttattt
      361 tgctgcggcg tcgctggcag agcctatgtg ctgcattatt ggtgcttatc atgccaatta
      421 tcacaccacg caatatgttt atgagcatcg catgggcgtc aaacctggcg gcaatattgc
      481 actgctggcg tgtgcgggtc cgatgggcat tggcgctatc gattacgcca ttaacggtgg
      541 catacaaccg tcacgggtgg tggtggtcga tatcgacgac aaacgcctgg cgcaggtgca
      601 gaagctgctg ccggtggatc tggcggccag taaaggcatt gagctggtgt atgtgaatac
      661 caaagggatg agcgatccta tccagatgct gcgggcgctg acaggagatg tcgggttcga
      721 tgacattttt gtttatgcgg cggtgcctgc tgtcgttgag atggctgacg aattactggc
      781 ggaagatggc tgtctgaact tctttgccgg gctgacggat aaaaacttca aagtgccgtt
      841 taatttctac aacgtccatt acaacagcac gcacgtagtc ggtacatccg gcggttcaac
      901 ggacgacatg aaagaggcga ttgcccttag cgccacagga cagttacagc cgtcgtttat
      961 ggtgacccat attggtggcc tggatgcggt gccagaaacc gtgctcaatc tgccggatat
     1021 ccctggcggt aaaaaactca tttataacgg cgtgaccatg ccgctcactg ccattgccga
     1081 ttttgccgaa aaaggcaaaa ccgatccgct gtttaaagag ttggcgcggc tggttgagga
     1141 aacgcacggc atctggaatg aacaggccga gaaatatctg ctggcacaat ttggcgttga
     1201 tatcggggag gccgcgcaat gatgtccctg gcgtggccat tatttcgcgt tacggaacag
     1261 gcagcgttgg ctgcctggcc gcaaaccgga tgtggcgaca aaaataaaat tgatggcctg
     1321 gcggtcaccg cgatgcgcca ggcattaaac gacgtcgctt ttcgtggacg agtggttatc
     1381 ggcgaaggag aaattgacca tgcgccgatg ctgtggattg gcgaagaggt gggcaaaggc
     1441 gatgggccag aagtcgatat cgcggttgat cccattgaag gcacgcggat ggtggcgatg
     1501 gggcagagca atgcgctggc ggtcatggct ttcgctcctc gagatagcct gttacatgcg
     1561 cccgatatgt atatgaaaaa gctggttgtt aatcgactgg ctgccggggc gattgatctg
     1621 tctttgcctc tggcggacaa cttgcgtaac gtggcgcggg cgttaggtaa accgctggac
     1681 aagctgcgca tggttacgct ggataaaccg cgtctgagtg ccgcgattga agaagcaaca
     1741 cagctgggcg tgaaggtttt tgccctgccg gatggcgatg tcgccgccag cgtgctgacc
     1801 tgctggcagg ataatcctta cgatgtgatg tacaccatcg gcggtgcgcc agaaggcgtg
     1861 atttctgcct gtgccgttaa agcgttaggc ggggatatgc aggccgagct gattgacttt
     1921 tgtcaggcta aaggggatta cacggaaaat cggcagattg cagagcagga gcgcaagcgt
     1981 tgtaaggcaa tgggcgttga cgttaaccgt gtgtactcgc tcgatgaact ggtgaggggg
     2041 aacgatatcc tctttagcgc tacgggcgtg acgggcggcg agttggtgaa cggtatccaa
     2101 cagacggcga atggggtgcg gacgcagaca ttactgatcg gcggcgcgga ccaaacgtgt
     2161 aatataatag actccctgca ttgatggtga tctatcggaa gaccaatggc gacgctgaca
     2221 gaagatgatg tgcttgagca actggatgca caggacaatt tattttcatt tatgaaaact
     2281 gcgcattcta ttttgctcca ggggatacgc cagtttctgc cgtcgctgtt tgtcgataac
     2341 gatgaagaga tcgtcgaata tgcagtgaag ccgttactcg cccaaagcgg cccgcttgac
     2401 gatattgatg ttgcgctgcg tttgatttat gcgctgggga aaatggataa atggctgtat
     2461 gccgatatca cgcacttttc ccagtactgg cattacctga acgaacagga tgaaacgccc
     2521 ggatttgccg atgatataac ctgggatttt atcagcaatg tcaacagtat aactcgcaat
     2581 gcgacgctct acgatgcgtt aaaagcgatg aagttcgccg atttcgccgt ctggtcagag
     2641 gcgcgtttta gcggaatggt caaaacggcg ctgacgctgg cagtaacgac caccttaaag
     2701 gaattaacgc cgtgaaaatt gaattaacgg tgaatgggct gaacgtgcaa gcgcagtacc
     2761 atgatgaaga gatagaacgc gtgcataaac tgttgctgcg tatgctggcg gccttacaga
     2821 cggtaaatcc gcagcggcgt acggtggttt ttctttgcgc tccgccgggg acgggtaaat
     2881 ctaccctaac caccttctgg gaatatctcg cgcaacaaga cccggagctg cccgctattc
     2941 aggcgctccc gatggatggt tttcaccatt acaatagctg gctggacgcg catcaattgc
     3001 gccccttcaa aggcgcacca gagacatttg acgttgcgaa actggcggaa aatctgcgcc
     3061 aggtggtgga aggggattgt acgtggccgc agtacgatcg acaaaagcat gatcctgttg
     3121 aagatgcgtt gcacgttacc gcaccactcg tcatcgtcga aggaaactgg ttgttgctgg
     3181 acgacgagaa gtggtgccag cttgcgcaat tttgtgattt ctcgatcttt atcaacgcgc
     3241 cagctgctgc gttgcgggag cgtctggtgg ggcgcaagct ggcgggaggg gtatcgctgg
     3301 cagatgcaga agccttttat gaccgtaccg acgggccgaa tgttcgccgg gggctggaag
     3361 agagtctgcc tgcaaatttg accttaatga tgaccgctac gggtgaatat cgtctggtgg
     3421 attaacgttc ctgattacac catcttccag ccgcaccaca cgagagaaat gacgccgggt
     3481 aaatgcggcg tcatgactga ttgctaccac gctcgtgcct cgctggcggc acttctccag
     3541 ccagctttca aacacgctca accaattttc gtcaaaatcc cgactcggct cgtccagcag
     3601 taatagtggc ggagagagtg cttccaggca ggcgacggcg accatccgtc gctgcgcatt
     3661 atgcagatcc aacgggtgcg agttggcagt gtctgctaac tgacaacatt gcagggcagc
     3721 atgggtacgt tgagtgattt catccgccgg gcatttttgc agtttcaaac caaatgcgat
     3781 ctcgtcagcg acggtgctgt gaaaaagttg gttttccgct tcctgaaaca gtacgccaac
     3841 ctttgccgcg cgctggcggt ttttcaggtt tttcatcgcc tgttgttgca gcataaccgt
     3901 accggctgtg ggcgtaagaa gacctgccat tactcgtaac agcgtcgatt tccctgcgcc
     3961 attgtcaccg gtcagcgcca accattcacc ttgtttgagt tgcagagaaa tgtcgcaaag
     4021 acaatccgtt gccgcgccag gccagcgata agatatctta tttaacgtta acatagtggc
     4081 tggagcgctc cgttttgcaa ttgccacgct tgctggcagt agttcaggaa aggtgtgcga
     4141 tgacgttcaa aaagaataat cagcgaacca cgttcaaacg cccagtgttg taagcgctgc
     4201 aacagcattt cgctggcctg cggtgtcagg cggctaaagg cttcatcaag aattaagagc
     4261 tttggctgca tcgcgatggc gcaggcgatg atcacgcgct gtgtctctcc accggagagt
     4321 gttgccgggt gacgatggcg caacggctgg cactcggtca gagtcagggc ggcatcaatc
     4381 cgcgccataa tctctgcttc agcaagacac agattttctg gcccaaaggc aacttcttct
     4441 tccacgctaa aggtacagcc ggagagctgc aaatagggcg attgctggac gagctggatg
     4501 gtggcggatt gttcattaag cgggagatgc ccaatgggcg taccgaggag cgtccccgtg
     4561 cctgttatct ctcttggcag aaagtccgga taccagcccg ccatcagctg tgccagcgta
     4621 cttttgccac tgccgttgtc accaaagatg gcgaccatcc ccggtttgac atagtgaaag
     4681 tcataacaga acggaggatg cgttgagtgt gtcgggcaat aacggaactg ttctaacgta
     4741 accatatcca cgctccgaat tctgtcagca tcagcaagat catggtgtaa cgcgctacgc
     4801 gttgtagggt gctatcggct ggtgcccaca acgttgttcg tcggttgtgg atccgaaatg
     4861 cccgcatatc cagcgccgca ccacggacag ccagatcgtt aagcgcattg tgtgttagcg
     4921 gaataattaa cgcgggcata gcacgtaaac gctgatacca gccctcatcc agcggcacgc
     4981 cgcgggcacg ctgcgcttca tgaataatcg ctaattgccg ttttaactgc tcaacgacca
     5041 ataacggccc ggcaaacaga taagcgacgc ctggcggcag gcgagaagca aacagagcgc
     5101 gaataaaaca ttgcacgggg acgtactgca ttcacaactg tgaggttgaa acaatcgcca
     5161 gaatcagcaa ccagagcgta atagcatgcg accaccgttc tggcgagcgt ggcgtgccgc
     5221 ttagccattc tggtagccag ccaccgtgta ccagccacag cccggctccc agcgagaaca
     5281 tcagccagac gacatatttt gcccgccgcc gcgtggcctt tagcgcaata aggcagaaaa
     5341 aggtcgccgc gctatagatc ggcaaaatgg tctgcgcggg caggataagc gtggtgcagg
     5401 ccgctaatgc ccacagtgtt aatgaggtaa atggatgcat ttagcgcacg gcagccattg
     5461 ccggaaaatg gcgcgtggtg cgaatcggca gctggcgcag taaaagccag acaatcaccg
     5521 ctgtaagaat tttatcgacc aggttcgcgc ctatcaccgt aatcgccacc gactccacca
     5581 ggttttgccc catcgagtgc atccaggcaa cgaacaaatc ggctccgcta ccggtgacac
     5641 cgccgaacag cgcagtacgc agcggtacag cgacgaccgt taccgcaagc gtaataatca
     5701 cgccgctgac aacgactttt ggcagggtgc gaaaccaacc cgctcgcgcc agccaaccgg
     5761 agaccaggcc gatgaccatc gcgacgggtg caaatgcggc ggcgatcggg tcggtcagca
     5821 atccccagag taaattggtc agtaagccgg tcagcattcc gatcaccgga ccaagcagta
     5881 ccgcgctaat aagtgtgccg atggagtcga gaaatatagg taatttgacc atgctggcca
     5941 gttgcccgcc gatcatattg atggcgatag atataacgat caacaccagt gcctgactgg
     6001 aaaagtggct acgcgccata agaaaacctc tttgaaagta gttatttaac gggggaaata
     6061 tgcaactcgt cgtaaccaga aagcgcgcaa ggttggcggc taaacgccag ttcctcaagg
     6121 ctaccgatca ccagttcaag cgtggtccgc acggtacagc cgggcatcat atgaccgcca
     6181 agcatcgtgc cgtgtggatc ggaaacgcag agatgaagat gctcaccact ctgttccagc
     6241 gtgccgttta gtgagatcac ttcgaatgta ccgttcagga gcgtggtgcc ttcttgcccg
     6301 gcatagcgca aggcgacgtc agtcaggcta ccggtacagc ctgcaatcta ggcggcatga
     6361 agttgatgtt gctgtgcaaa agcgtgtagc tgggaaaaaa cttcctgccc ggggaggagg
     6421 cgcagtgcgt aaaagcgtgc ggaggaagaa tatgagtagg gcaatgtcat cattggagta
     6481 ctccgaaact cggacgtatt gcggataata tcgacgctgc atcagtcata ttctgatata
     6541 agtcagggtt tgtgacggca caacgaaaag attttgctac gcttattcgt gattctgcaa
     6601 agtggaataa attccacagg ggcggggctg aaaacgtgac gtaagtcaga taattgcttt
     6661 ctttgactgg acaatcattc cttttattcc acgtttcgct tatcctagct gaagcgtttc
     6721 agtcgattaa atgttcaaca attaaccaat cagtcgcagt ttgcgacagg taaggtttcc
     6781 ccggacgatt tgctggatta ctctgtctgg caaatttgct cagggaaaac cttgcaggag
     6841 atctatgacc gtacgcgtag cgataaatgg cttcggtcgc atcgggcgta atgtggttcg
     6901 tgctttgtat gaatccggac gccgggcgga aattaccgtg gtggcaatca acgaactggc
     6961 ggatgctgcg ggcatggcgc atttgttgaa atatgacacc agccatggcc gttttgcatg
     7021 ggaagtacga caggaacgcg atcaactttt tgttggtgat gacgccatcc gcgtattgca
     7081 tgaacgttca ctgcaatcgc tcccctggcg tgaacttggc gttgatgtag tcctcgactg
     7141 caccggcgta tatggctccc gcgagcatgg cgaagcgcat attgccgccg gggccaaaaa
     7201 agtgctcttt tcacatcctg gcagtaacga tctcgacgcg accgttgttt acggcgtcaa
     7261 tcaggatcaa cttcgtgcgg aacaccgcat cgtttctaac gcttcctgta ccacgaattg
     7321 cataattccc gtcatcaaat tgttagatga tgcgtacggt attgagtccg gcactgtgac
     7381 cacaattcac tccgccatgc acgatcaaca ggttattgat gcataccatc ctgacctgcg
     7441 tcgcacccgg gcagccagcc agtcgatcat tccggtcgat actaaactgg ccgctggtat
     7501 cacacgattt tttccgcaat ttaacgatcg ctttgaagcg attgcggtac gtgtgccaac
     7561 cataaatgtg acggcaatcg atttaagcgt gacggtgaag aaacctgtaa aagccaatga
     7621 agtcaacctg ttgctgcaaa aagcagcaca aggtgcattt catggtatag ttgactatac
     7681 ggaattgccg ttggtctctg tagattttaa ccacgatccg cacagtgcca ttgtcgatgg
     7741 cacccaaacc cgggtcagtg gcgcacacct gatcaaaacg ttggtctggt gcgataacga
     7801 atggggcttt gctaaccgaa tgctcgacac gacgttagct atggctactg ttgctttcag
     7861 gtaagacgca agcagcgtct gcaaaacttt tagaatcaac gagaggattc accatgtctg
     7921 taattaagat gaccgatctg gatcttgctg ggaaacgtgt atttatccgt gcggatctga
     7981 acgtaccagt aaaagacggg aaagtaacca gcgacgcgcg tatccgtgct tctctgccga
     8041 ccatcgaact ggccctgaaa caaggcgcaa aagtgatggt aacttcccac ctgggtcgtc
     8101 ctaccgaagg cgagtacaac gaagaattct ctctgctgcc ggttgttaac tacctgaaag
     8161 acaaactgtc taacccggtt cgtctggtta aagattacct cgacggcgtt gacgttgctg
     8221 aaggtgaact ggttgttctg gaaaacgttc gcttcaacaa aggcgagaag aaagacgacg
     8281 aaaccctgtc caaaaaatac gctgcactgt gtgacgtgtt cgtaatggac gcattcggta
     8341 ctgctcaccg cgcgcaggct tctactcacg gtatcggtaa attcgctgac gttgcgtgcg
     8401 caggcccgct gctggcagct gaactggacg cgctgggtaa agcactgaaa gaacctgctc
     8461 gcccgatggt ggctatcgtt ggtggttcta aagtatctac caaactgacc gttctggact
     8521 ccctgtctaa aatcgctgac cagctgattg ttggtggtgg tatcgctaac acctttatcg
     8581 cggcacaagg ccacgatgtg ggtaaatccc tgtacgaagc tgacctggtt gacgaagcta
     8641 aacgtctgct gaccacctgc aacatcccgg ttccgtctga tgttcgcgta gcaaccgagt
     8701 tctctgaaac tgcaccggct accctgaaat ctgttaacga tgtgaaagct gacgagcaga
     8761 tcctggatat cggtgatgct tccgctcagg aactggctga aatcctgaag aatgcgaaaa
     8821 ccattctgtg gaacggtccg gttggcgtgt tcgaattccc gaacttccgc aaaggtactg
     8881 aaatcgtggc taacgctatc gcagacagcg aagcgttctc catcgctggc ggcggcgaca
     8941 ctctggcagc aatcgacctg ttcggcattg ctgacaaaat ctcctacatc tccactggcg
     9001 gcggcgcatt cctcgaattc gtggaaggta aagtactgcc tgcagtagcg atgctcgaag
     9061 agcgcgctaa gaagtaaaaa atcacagggc agggaaacct gcacttgttt cagcgcgctt
     9121 ttagagcacg cattttttca tactctaaat aattcgagtt gcaggaaggc gacaagagag
     9181 tgaatcccca ggagcttaca taagtaagtg actggggtga gcgaacgcag acgcagcaca
     9241 tgcaacttga agtattatga gtataaggcc cgacgataca ggacaagaga catgtctaag
     9301 atttttgatt tcgtaaaacc tggcgtaatc actggtgatg acgtacagaa agttttccag
     9361 gtagcaaaag aaaacaactt cgcactgcca gcagtaaact gcgtcggtac tgactccatc
     9421 aacgccgtac tggaaactgc tgctaaagtt aaagcgccgg ttatcgttca gttctccaac
     9481 ggtggtgctt cctttatcgc tggtaaaggc gtgaaatctg acgttccgca gggtgctgct
     9541 atcctgggcg cgatctctgg tgcgcatcac gttcaccaga tggctgaaca ttatggtgtt
     9601 ccggttatcc tgcacactga ccactgcgcg aagaaactgc tgccgtggat cgacggtctg
     9661 ctggacgcgg gtgaaaaaca cttcgcagct accggtaagc cgctgttctc ttctcacatg
     9721 atcgacctgt ctgaagaatc tctgcaagag aacatcgaaa tctgctctaa atacctggag
     9781 cgtatgtcca aaatcggcat gactctggaa atcgaactgg gttgcaccgg tggtgaagaa
     9841 gacggcgtgg acaacagcca catggacgct tctgcactgt acacccagcc ggaagacgtt
     9901 gattacgcat acaccgagct gagcaaaatc agcccgcgtt tcaccatcgc tgcgtccttc
     9961 ggtaacgtac acggtgttta caagccgggt aacgtggttc tgactccgac catcctgcgt
    10021 gattctcagg aatatgtttc taagaaacac aacctgccgc acaacagcct gaacttcgta
    10081 ttccacggtg gttccggttc tactgctcag gaaatcaaag actccgtaag ctacggcgta
    10141 gtgaaaatga acatcgatac cgatacccaa tgggcaacct gggaaggcgt tctgaactac
    10201 tacaaagcga acgaagctta tctgcagggt cagctgggta acccgaaagg cgaagatcag
    10261 ccgaacaaga aatactacga tccgcgcgta tggctgcgtg ccggtcagac ttcgatgatc
    10321 gctcgtctgg aaaaagcatt ccaggaactg aacgcgatcg acgttctgta agtcattcct
    10381 ttctgcttat ctcaaggccc gctctgcggg tctttttttc gccaaaagca aggtgattca
    10441 gatgagaaat ctgtgatcta tttggcaaaa ttatgcttta ttgtttaccc ttgtcagact
    10501 gcccgtcata aggcggcgga gtgtatttct ccattttgag tcagttgaaa aggaatattg
    10561 aatggaagat ttgaatgttg tcgatagcat aaacggcgcg ggaagctggc tggtagctaa
    10621 ccaggcgctg ctgctaagtt atgcagtaaa catcgtggcg gcactcgcga tcatcatcgt
    10681 tggtttgatt atcgcgcgga tgatttccaa cgcggtgaat cgcctgatga tctcccgtaa
    10741 aatcgatgcc actgttgctg attttctttc tgcattagtc cgttacggta ttatcgcctt
    10801 tacgctaatc gctgccctgg gacgcgtggg tgtacaaaca gcgtcagtca ttgctgtact
    10861 cggtgccgca ggcttagctg ttggtctggc tttgcagggg tcactttcta acctggccgc
    10921 tggcgtgtta cttgtcatgt tccgcccgtt ccgtgccgga gaatatgttg acctcggcgg
    10981 cgtagccggt actgtgctga gtgtgcagat tttctccacc accatgcgta ctgcagacgg
    11041 taaaattatc gttattccga acggtaaaat tattgccgga aatattatta acttctcccg
    11101 cgagccagtt cgccgtaacg aatttattat tggcgtggcg tatgattccg atatcgatca
    11161 ggttaagcag atcctgacca atattatcca gtctgaagat cgcattttga aagatcgcga
    11221 aatgactgtg cgcctgaacg aacttggtgc atcgtcgatt aatttcgtgg tccgcgtctg
    11281 gagcaacagc ggcgatctgc aaaacgtgta ctgggatgtg ctggagcgta ttaaacgtga
    11341 atttgatgcc gccggtatca gcttcccgta cccgcaaatg gatgtgaact ttaagcgggt
    11401 gaaagaagac aaagctgcgt aatcaacgct gaggccagat aatactctct ggcctctctc
    11461 ttattagtta ttctgattgc caattaatat tatcaatttc cgctaataac aatcccgcga
    11521 tatagtctct gcatcagata cttaattcgg aatatccaac gtgttttctt attactttca
    11581 aggtcttgct cttggggcag ctatgattct gccgctcggc ccacaaaatg cttttgtgat
    11641 gaatcagggc attcgtcgtc agtaccacat tatgattgcc ttactttgcg ctatcagcga
    11701 tttggtcctg atttgcgcgg ggatttttgg tggtagcgcg ttattgatgc agtcgccgtg
    11761 gttgctagcg ctggtcacct ggggcggcgt agtcttcttg ctctggtatg gttttggcgc
    11821 ttttaaaaca gcgatgagta gtaatattga gttagctagt gccgaagtcc tgaagcaagg
    11881 cagatggaaa attatcgcca ccatgttggc agtgacctgg ctgaatccgc atgtttacct
    11941 ggatactttt gttgtactgg gcagccttgg cgggcagctt gacgtcgaac caaaacgttg
    12001 gtttgcgctc gggacaatta gcgcctcttt cctgtggttc tttggtctgg ctattctcgc
    12061 agcctggctg gcaccgcgcc tgcgcacggc aaaatcacag cgcattatca atctggtagt
    12121 gggatgtgtc atgtggttta tagccttgca gctggcgaga gacggtattg ctcatgcaca
    12181 agccttgttc agttaggcgc tatctgatgg aaaaataaaa cagaggcgct aagcttgcct
    12241 ccagaggtcc tgaattttgg gcaagtcaat taacacggag agactaacgt gaagttcaaa
    12301 gttatcgccc tggcggcatt aatgggtatt agcgggatgg cagcgcaggc taacgaattg
    12361 ccggatggac cgcatattgt cacctccggt acggcaagtg tggatgcggt gccagacatt
    12421 gccactcttg cgattgaagt taacgtggcc gcgaaggatg ccgctacagc caagaaacag
    12481 gcagatgagc gcgtcgcaca atacatttcc ttccttgaac tcaatcagat cgcgaaaaaa
    12541 gatatcagct cagcgaattt acgcacccag ccagattatg actatcagga tggtaaaagt
    12601 atcctgaaag gctaccgagc tgtgagaacg gtggaagtca cgctccgtca gttagacaaa
    12661 ctgaattcct tgctggatgg cgcgctgaag gcgggtctta acgaaattcg ttctgtgtcg
    12721 ctgggcgtgg cgcagccgga tgcctataaa gacaaagcgc gtaaggcagc gattgataac
    12781 gcgattcatc aggcgcagga actggcgaac ggctttcatc gtaaactggg gccggtatat
    12841 agcgtgcgct accatgtttc caactatcag cccagcccaa tggtgcggat gatgaaagcc
    12901 gatgccgcgc cggtgtctgc ccaggaaact tacgagcagg ccgctattca gtttgatgat
    12961 caggtcgatg tggtcttcca gttagaacct gtggatcaac aacccgctaa aacacctgca
    13021 gcacaataat acttgtagcc cgacgtattc gtcgggctaa tgcctttacc cgatattgcc
    13081 tcctttttct gtaattttct gtaaaaccat gacgtaatca cctatctttg ctcgcggttt
    13141 catcaaggat aggctgactg aaacggttaa gcggacaagg atgcggggag gggggatgga
    13201 catttttatt tcgaaaaaga tgcgcaattt cattttatta gcgcaaacca ataatattgc
    13261 ccgggcggca gaaaaaatcc atatgacggc ttcgccattt ggtaaaagca ttgccgcgct
    13321 ggaagaacaa attggctata cgctatttac ccgcaaagat aataatatca gcctcaataa
    13381 agccggtcag gaactgtatc aaaaactgtt cccggtttat caacgacttt ctgcgattga
    13441 taatgaaatc cataactcag ggcgtcgttc acgagaaatt gtgattggca tagataatac
    13501 ttatccgaca attattttcg atcagttgat tagtctcgga gataagtatg aaggcgtgac
    13561 cgcccagcca gttgagttca gcgaaaacgg cgttatcgat aatctttttg accgtcagtt
    13621 agattttatt atctccccac agcatgtatc tgcacgagtt caggaactgg aaaacctgac
    13681 tatcagtgaa ttaccaccgc tgcgattggg ctttctcgtt tctcgccgtt atgaagagcg
    13741 tcaagagcaa gagctgcttc aggagctgcc ctggctacaa atgcgctttc aaaaccgggc
    13801 taacttcgaa gcaatgattg atgcgaatat gcgaccttgt gggatcaatc ccaccattat
    13861 ttatcgtccc tacagtttta tggccaaaat cagcgccgtg gagcgcggac attttctgac
    13921 ggtgatcccg cattttgcct ggcgactggt gaatccggca acgctgaaat attttgatgc
    13981 gccgcacagg ccgatgtata tgcaggaata tctttattca atcagaaatc atcgttatac
    14041 cgccacgatc ttcagcatat tgctgaagat cgtgacggga caaaccatta acccagcatc
    14101 gagccggttg caattaaatt acggtgtaag tcgaagacgt ggctaagatc gtggtgaata
    14161 tgtccgccgg gcgcattttc cagatagcga tgcagataat cccgatacat aggatgtgca
    14221 caattatcaa tgatagtgcg ggcgcgttga agcggggaaa gaccgcgcag atcggcgatc
    14281 ccttgttcgg tgatgatcac tttgacgctg tgttcgctgt gatcaacatg gctgcacatt
    14341 ggcacgacgg ttgagatctt gccttcttta gcaatcgacg gagccataaa gatcgacaga
    14401 tacgcgttgc gttcaaaatc accgctgccg ccgatgccgt tcatcagatc gaccccggct
    14461 acgtgcgttg aatttgcatg cccgtaaata tcaaactcca ggccgacgtt cagagcgatg
    14521 acgcccagac gacggatgat ttccgggtta ttggaaatct cctgcggacg caacacaatg
    14581 cggctggcaa agtaatccat attgtcgtaa atcttgcgca gggaatcggc cgagattgtc
    14641 aggctggagg cgctggcccc gctgattttg ccggtttcca gtaaatgcac caccgattcc
    14701 tgtagcactt ccgaatacat catgaacgga ggaatttccg ggttttcccc cagacgtgcc
    14761 attaccgcat tattgatatt gcccacgcca ctttgcagcg gcagaaattc cggcggaata
    14821 cgcccatgcg ccatttcctg caataagaac gtgaccacgt tatcggcaat ctgctggcac
    14881 atgggatttt gcttatccag catattaccg gcgtcgggca agttggtttc cacgacggcg
    14941 acaatctttt tcggatcgat ttgcacatag cgggtaccga cgcgatccat tgcatggaag
    15001 atcgacacgc tattgcgccg cggtggcgcg ccaggaatca caatatctgc cagttctgca
    15061 acgcgcggat cgtgatagtg attaagttca atgatcactt tcttcgctcg cagcagctag
    15121 gtcggcgcat taccgatccc gctggttaac cagactcgac catccggtgc cagtgccgat
    15181 gcttcaatga cggcaacatc aatgtcgccg aagaaaccgt aattgaccat ttgcgccact
    15241 tcgctcaaat gcaggtcaac gaaactcacc gcgccctgat tgatcttttt acgtaaactg
    15301 gacgatgttt gatatggcgc acgccaggaa acagcatcgg cgtcagaaag tacatcgtca
    15361 gcggcggcgc tgattgacgc ccccgtcagc aggcgaattt gatacggctt tttggcctca
    15421 tgctgttcgt tagctctgcg ggcaatcgcg gcgggtaggg ctttcggcga acccgctggg
    15481 gtaaagccgc taaatgccac catgtcgtta tgctggataa tttctgccgc ttcatcggcg
    15541 gtcatccttg tccactgagt ttccattttt actccttcgt tcgcagggat taatgaccaa
    15601 cgaaattagg tttacgtttt tcgaggaaag cgttcatccc ttcctggtaa tcttcgctgt
    15661 cataaaccgc gcggcgcatc ccctgaatac gttcaaattc atcggagttc atggtgtgtg
    15721 cttcgcccag tacacgcagc tcttctttga taacggcaat ggctaacggc gctttctcag
    15781 agatgtggtg cgccatttgt aaggtgaaat cttccagttc ttccacttcc acaacatggt
    15841 tgaggatgcc gacagccagc gcgcgctggg cggtgattgg cgaagcggta aaaatcagct
    15901 ctttgacaat gtggaagccc gcgtcgcggg tcaggttgtg aatgccgacc aggttatacg
    15961 ggacgccgag gtttacaggc gtcattgaga aggttgaggt actggcggcg atgatcagat
    16021 cggaactcat gatcatttca aatgcgccac cccaaacact accttccacc atcgaaatga
    16081 tcggtttcgg gaatttttgg atcatgcggg tgatttgacg caatggatca tcataggaga
    16141 gcggatcgcg accgccagac ggcagttcgt gaatatcgtg acctgcggag aagactttgg
    16201 atccacttgg cgcccgcaaa atgatacagc gaatttccgg ccggttgaga tcgcttaacg
    16261 cctgcataag atcatcaata aagactttac ttaaggcatt aagttttcgt ccatagttaa
    16321 actcaatgac cgccactttg ttgatagtgg caacgttcac atactgataa gacataaaaa
    16381 ttcctttaat caaaatattg cgtctggata aattcactga gctgccgcag gccggtgcgc
    16441 ggtgagagcg tattgttttt gaccgctaaa agcgtctggc ggtaatagcg atcgaaatct
    16501 tcattcgcga acaggtgatt cagtacttct tcttcggtct gcttacgcag ccattccacc
    16561 gattgttgtt gccgcacttg ttgtaaacga ccactggcag ttagcgcggt tttgaagtcg
    16621 atgatggcgt gccagatctc atcgattcca cgtttttcca gtgcgctaca agtcagaccc
    16681 cgtggctgcc attcgtcgta tttacgtcgc agaatatgca gggcactctc gtacatatgc
    16741 cgggcaatgg cgacattggt atggttatcg ccatcgtctt tgttgataac gagcagatca
    16801 gccacttcca tcagcccttt tttaatgccc tgcagatcat cgccgccacc ggcaatttgc
    16861 aacgagataa aacagtccac catgcgggcg acttccgttt ccgactgcgc gatgccaacc
    16921 gtttcgacaa tcactacgtc ataacccgcc gcttcgcata acagcattaa ttcccgtgct
    16981 cgctgactgg caccgcccag atgaccggag gagggtaccg ggcgaataaa cgccgcctcg
    17041 gcacgcgcca ggtcattcat gcgggtttta tccccgagaa tgctaccgcc agtgaccggg
    17101 ctgctgggat cgaccgcaat aacagcaacc tttaatccct cacgaatcaa caacatgcca
    17161 aaggcttcaa gaaaggtact tttccccgcg ccgggtgtgc cggtaacgcc cagtcgcagg
    17221 gcgttaccac agaacggcat aatagcgtca agcagctgtg tacttagcgc ctgatgacgt
    17281 gggtgacggc tttccaccag cgtcatggct tgggcgagcg tggcacgctc accctgacgt
    17341 aagcggcgaa tactttctgc cagcgtggct tcattaatca tgatgctggc ttatcagatt
    17401 cagtacgtcg cgcacactgt cgagcatagg tgtacctgga ccataaatcg ccgccacgcc
    17461 gcgctcttgc aggaaggcgt aatcctgcgg cggaatgacg ccacccgcga ccacgcagat
    17521 atcttcgcgt ccccattttt tcagcgcttc gaccagttcc gggatcagcg ttttatgacc
    17581 ggcagccagt gaggatgcgc ccactacgtg aacgtcgttt tctacggcta ggcgggcgat
    17641 ctcttcaggt gtagagaaca tcgggcttaa atctacgtcg aaaccgagat cggaataggc
    17701 gctggcgatc actttcgcgc cgcgatcgtg tccatcctgg cccatcttag cgatcagaat
    17761 gcgcgggcga cgaccattgt cggcaaggaa ctgctccgtt tgcgcaacaa tggcatcgaa
    17821 ctcggaggcc gatttctcag actgatgata gctttgcgca atcacgccgg taacacactg
    17881 gcttggcacc agataacggt cgaaagcgac ttccagcgca tcggaaattt cacccagggt
    17941 ggcgcgaacg cgagcggcat taacagcggc agccagcagg ttttcgttat gctgtgcggc
    18001 gtgagtcagg gcgttcaacg cggcggttac ggcggcatca tcacgggtgg cgcgaatgcg
    18061 ttccagcgaa gcaatttgct cgttacgcac catcacgttg tcgatctcaa gtacatcggt
    18121 ttcgtcttcg tgatccagtt tgtacttgtt gacaccaacg atgacacgct tgccctggtc
    18181 gatcagcgac tgttcgcgcg ctgaggcctc ttcgatcatt cgttttggca gacctgcttc
    18241 gatcgctttc gccatgccac cggcttcgtc gatctgttgg ataatagctc tggcttgttt
    18301 gacgatttga tcggtcagcg actcaatgta ataggatccg gccagtggat cgacggtgtg
    18361 gcagagttct gattcttcct ggatgatgat ctgggtgttg cgggcaatgc gtgctgagaa
    18421 atcggtaggc aaaccaagcg cttcgtcaaa ggcgttggta tgcagtgact gagtaccgcc
    18481 cagcgtcgca gccagtgctt caatggtggt gcggataacg ttgttatacg gatcctgttc
    18541 agtcaggctc cagcctgagg tctggcagtg ggtacgcagc gccagtgatt tcggatcctg
    18601 tgcgccaaat ccactgaccg cttcgctcca taaataacgt gccgcacgca acatggcgac
    18661 gttcataaac agatccatgc cgataccgaa gaataacgac aggcgaggag cgaagtcatc
    18721 aattttcagt ccggcagaga ttgctgcttt gatgtactca atcccatcgg cgagcgtaaa
    18781 tgctacctgc tgcacgcagt tggcacccgc ttcccccatg tggtaaccgc tgatactgat
    18841 ggtattaaat cgcggcatgt tgccggaaca ccaggcgatg atgtcggcga taatgcgcat
    18901 tgacggtttt ggtgggtaaa tataggtgtt gcggcagagg tactctttga gaatatcgtt
    18961 ttgaatggtg ccggtcagtt tatcaggtgt aacaccttgc tcttctgcgg cgacgatata
    19021 aaacgccagt actggtagca ctgcgccatt catggtcatc gaaaccgaca ttttatccag
    19081 cgggatctgg tcgaacagga ctttcatatc ttccacggtg tcgatagcga cgcccgcttt
    19141 gccgacgtca cccgccacgc gtgggttatc ggagtcgtag ccacggtggg tggcaaggtc
    19201 aaacgcaacg gaaagacctt tttgcccggc ggccaggtta cggcgataaa aagcgttgga
    19261 ctcttttgct gttgaaaaac cagcatactg acggatggtc cacggttggg cggtatacat
    19321 agtggcacgc gggccacgaa cgtagggcgg caaaccagga agggtacctg tcacctccag
    19381 attatcgaga tcggcttcgg tatacagcgg cttgatggcg atcccttccg cggtttgctg
    19441 aaccagcgag tcgacagttt tctcccgacg gctcaattcc ttgttggcaa gctgttgcca
    19501 ctcctgctcg ttagacatta tttgctccgt aaaacgcaat caaaatgagg gtatttgcgc
    19561 ttacggtgag ctttttacgt gggtttgtcg ccagcaaaaa aaatgcattc agacgtcgtt
    19621 ttttgccgcc tgagatttgt ctattttgtg atgtgattca aggaatacaa aaagaagtga
    19681 ggacaacgcc tgatatgtgc tatcaggcat tatttgatgg attaatcctg acgaaggact
    19741 ttgtgaccat aatcgagtaa cgcatcagtg actttacgca tcatgcggct ttcaggagca
    19801 aagcggtgcc agtagagcat ccgtcgttga aatagcccag gcgttaagtc aatcagttca
    19861 ccgctggcca gctctttctc gatttgcagg tgcgggatca tacagcaggt ggtgccctgg
    19921 cgagcaagtt gtacgaacgc ttctgaagaa ttaacgatat ggcagggtac gctgccagga
    19981 ggaagatcga agttttgctg caaaaaggcc tggtgcatat cgtcaagatg gtcaaacgcg
    20041 accactggcg ctttcaataa tgccgaacgc gttacgccgt taggaaaata tttttcggca
    20101 aacggttttg agctgacgaa cagatagtcg agcgcaccga gtttatcgac aagacaactc
    20161 ggcagcgcct gatgttgaat actcaccgcg ccgaccactt cgccgcggcg cagacgttcc
    20221 tgagtacggg tttcatcttc tacctgcaag ttgaggcgga taggcgaatc agccaacaca
    20281 ggagccagtg ccggaagcaa ccacgtcgcc agactgtcgg cgttgaccgc cagtgaaagc
    20341 agcagcggag tcgaaccggt ttgttcatcg cccagccact cttcttccag caactccacc
    20401 tggcgcagca gtgccagcag tttttgccct tgttccgtcg ggcgcggcgg tacggtacgc
    20461 accaacagcg gctgcccgaa catattttcc agttgcttaa tgcgctgtga gacggctgat
    20521 tgtgtaatgc acagcttttg tgccgcgcgc tcaaatcctc gttcacgtat caccgcatcc
    20581 agtgcctgta atgttctgta gtccgggcgt ttcattgctg ttgtctgctc ctggtaaatg
    20641 ctttttcctg cactatgaca caattttgtg tcaggttgca tataccgaag ccgtcggcgg
    20701 gtaacatttg tatcaatggt taaacgcgca gcttaaaaga tcagggattt gcgctattgc
    20761 atttcgctta gcgacttcgc gccctggtta tgttgaatgg cgtggcgtta ttgcctctgt
    20821 tcgcaccgtg gagcggcata cccagaccgt tgtatgcttt atttgcgggg cgaaagggga
    20881 tgcccgccat tgcgcgatat tttatggatg agttaaccac ccggcttgcc aacggggtct
    20941 gaatcgcttt ttttgtatat aatgcgtgtg aaatttcata ccacaggcga aacgatcatg
    21001 acgcaggatg aattgaaaaa agcagtagga tgggcggcac ttcagtatgt tcagcccggt
    21061 accattgttg gtgtaggtac aggttccacc gccgcacact ttattgacgc gctcggcacg
    21121 atgaaaggcc agattgaagg ggccgtttcc agttcagatg cttccactga aaaactgaaa
    21181 agcctcggca ttcacgtttt tgatctcaac gaagtcgaca gccttggcat ctacgttgat
    21241 ggcgcagatg aaatcaacgg ccacatgcaa atgatcaaag gcggcggcgc ggcgctgacc
    21301 cgtgaaaaaa tcattgcttc ggttgcggaa aaatttatct gtattgccga cgcctccaag
    21361 caggttgata ttctgggtaa attcccgctg ccagtagaag ttatcccgat ggcacgtagt
    21421 gcagtggcgc gccagctggt gaaactgggc ggtcgtccgg aataccgtca gggcgtggtg
    21481 accgataatg gcaacgtgat cctcgacgtc cacggcatgg aaatccttga cccgatagcg
    21541 atggaaaacg ccataaatgc gattcctggc gtggtgactg ttggcttgtt tgctaaccgt
    21601 ggcgcggacg ttgcgctgat tggcacacct gacggtgtca aaaccattgt gaaatgatct
    21661 gacgggggaa cctcccccgt taaaaaaatt ctcttcatta aatttggtga catgtgtcac
    21721 gcttttacca ggcaattgtc gattgctcta aataaatcct ctaaaccagc atattcatcc
    21781 aagaattacc tttgcgtgat atttcctcaa catcgcgacg caaacgttca tattgccgca
    21841 atattatttt ttgatatgtt gaaaggcgga tgcaaatccg cacacaacat ttcaaaagac
    21901 aggattgggt aaatggcaaa ggtatcgctg gagaaagaca agattaagtt tctgctggta
    21961 gaaggcgtgc accaaaaggc gctggaaagc cttcgtgcag ctggttacac caacatcgaa
    22021 tttcacaaag gcgcgctgga tgatgaacaa ttaaaagaat ccatccgcga tgcccacttc
    22081 atcggcctgc gatcccgtac ccatctgact gaagacgtga tcaacgccgc agaaaaactg
    22141 gtcgctattg gctgtttctg tatcggaaca aaccaggttg atctggatgc ggcggcaaag
    22201 cgcgggatcc cggtatttaa cgcaccgttc tcaaatacgc gctctgttgc ggagctggtg
    22261 attggcgaac tgctgctgct attgcgcggc gtgccggaag ccaatgctaa agcgcaccgt
    22321 ggcgtgtgga acaaactggc ggcgggttct tttgaagcgc gcggcaaaaa gctgggtatc
    22381 atcggctacg gtcatattgg tacgcaattg ggcattctgg ctgaatcgct gggaatgtat
    22441 gtttactttt atgatattga aaacaaactg ccgctgggca acgccactca ggtacagcat
    22501 ctttctgacc tgctgaatat gagcgatgtg gtgagtctgc atgtaccaga gaatccgtcc
    22561 accaaaaata tgatgggcgc gaaagagatt tcgctaatga agcccggctc gctgctgatt
    22621 aatgcctcgc gcggtactgt ggtggatatt ccggcgctgt gtgatgcgct ggcgagcaaa
    22681 catctggcgg gggcggcaat cgacgtattc ccgacggaac cggcgaccaa tagcgatcca
    22741 tttacctctc cactgtgtga attcgacaac gtccttctga cgccacacat tggcggttcg
    22801 actcaggaag cgcaggagaa tatcggcttg gaagttgcgg gtaaattgat caagtattct
    22861 gacaatggct caacgctctc tgcggtgaac ttcccggaag tctcgctgcc actgcatggt
    22921 ggtcgtcgtc tgatgcacat ccacgaaaac cgtccgggcg tgctaactgc gctgaacaaa
    22981 atcttcgccg agcagggcgt caacatcgcc gcgcaatatc tacaaacttc cgcccagatg
    23041 ggttatgtag ttattgatat tgaagctgac gaagacgttg ccgaaaaagc gctgcaggca
    23101 atgaaagcta ttccgggtac cattcgcgcc cgtctgctgt actaattccc cttctctgaa
    23161 aattaacggg caggtcactg acttgcccgt ttttttatcc cttctccaca ccgttgccgc
    23221 gttgcgcatt ctactttgcg agtctgctcc agaaaataaa tcaaagtcag atcaatgcgt
    23281 tgtgtttatt taatggggat ttacgatggc gggacagcat ggggctgtaa cggtaaagcc
    23341 ctcaccgaag cgagggctta aaggagaagg gttatgatgc gacttgtcat catactgatt
    23401 gtactgttac tgataagttt cggagcttat taacagtcag tctcaaggga ggagtcatcc
    23461 tcccttaccc ttactcacta aattaggtca aagaatcaac gatgtcaatc agggcgatgc
    23521 ggggtgcatc gccctgaacc gaactttttg atagccactt catcgcccac gtttactgcc
    23581 gtaatagatc ctttttccca aaaattatct ctgttttgca tggggttttt cgagcacgct
    23641 cgaagaaaag atttaaaggt agatcaatgc gttgtggtta tttaaagaga gcttacgata
    23701 gctgggcagc atggggctgt aacggtaaag ccctcaccga agcgagggct tgaaggagaa
    23761 gggttatgat gcgacttgcc atcatactga ttgtactgtt actcataagt ttcccagctt
    23821 attaacagtc agtcacaggg gaggagcaat cctcccttac ccttactcac taaattaggt
    23881 taaagaatca acgatgtcaa tcagggcgat gcaggtgtat cgcccttacc actcccagac
    23941 tttcgacggt gtaaccaccg caggaagagg gatatcccac tcttcaacgg ggagtttttc
    24001 caccaactga caatcatgcg cataacccac cggttgcgtt ttatagtgct gccagttttg
    24061 taaggtccga tcataaaaac caccgcccat tcccagacgc tgaccgtact catcaaaggc
    24121 gaccagcggt gtgatcagca cgtctaatcg ggaaaggggt agcacgtcac gcacatccag
    24181 ttttggctca tggatcttca acctgttcat caccagttcg ctttgcggat ggtaattcag
    24241 gaacagcaaa ttaccggcac taaagggatg caaaactgga agatatacgc gcttaccggc
    24301 gcgccagagt tgttctatta gcggctgggt gtcgagttca ccatcaaaag agaggaatac
    24361 agcgaccgta tgcgccatca ccaccggggg ataagtcatc atccgggtag cggcttgttg
    24421 acccatttcc agctgttgtt ccggcgttaa cgcacgacga cgttgccgaa tcattttgcg
    24481 gatttcttgt cgggataatg tcagtgggag ctctggtagt tgcgtcatgg ctggtgccag
    24541 ataagaaggg aatctccgag atgccgccgc aggctgtaac ccttgaaccc ttggttcaag
    24601 gtgaatgtgt cgtcgcagtt ttaaggcttc tcggacgaac cgagcatgct caccaaccgc
    24661 ggagcgccac attcttgtgg tatgaaatat cggctcaggg gactggcccg cttgcgaaca
    24721 tctcagagaa attttgtctt cacggttact ctaccacagt aaaccgaaaa gtgtcattca
    24781 aagttttggt tagttttttc ggtgatgcga ccttgttcaa gtaacgcttg ttctatggtc
    24841 tgctgcagca tccgaatacg ctgttccata cttgccgcgt agtcacgagt ctttgctttt
    24901 tcttgcgcta actcatagct gatattcaat gcggcaatga agaccaactg ttcagtattt
    24961 gtgactctag tgcgttcttt cagatcttgc aaccgttggt tcagatcgtc cgctgcctga
    25021 ttcaacgcat ccctttggtc aggcgggcag ttcacacgca gtgaacggcc aaaaatttgg
    25081 atatcgacgg gttgtgcaga catgccacct tcctgctgat tgactgcgct gccttcgtct
    25141 ttagacccat ggtctgcgaa ggggcgacac tatagctacc ctgatgagaa gagacaagcc
    25201 cttttctggt ccaccagggc ccaaagtggt agcatatcat gaatattcct ccctttgacg
    25261 acgaatgctt atgtctatac agaacgaaat gcctggttac aacgaaatga accagtatct
    25321 gaaccaacaa gggacgggtc tgacccctgc cgagatgcat ggtttaatca gtgggatgat
    25381 atgtggcggt aacgatgaca gctcatggct gccgctactt cacgacctga cgaacgaagg
    25441 catggctttc ggtcatgagc tggcacaggc actgcgtaaa atgcactctg ccaccagcga
    25501 tgccctgcag gatgacggct tcctttttca gctttatctg cctgatggcg atgatgtcag
    25561 cgttttcgat cgggctgatg cactggcagg ttgggtcaat cacttcctgc ttggtcttgg
    25621 cgttacgcaa ccgaagctgg acaaagtgac cggcgaaacc ggtgaagcca tcgacgatct
    25681 gcgtaacatc gcgcagctgg gctacgacga agacgaagat caggaagagc ttgaaatgtc
    25741 gcttgaagag atcatcgaat acgttcgtgt tgccgcgctg ttatgccacg acacctttac
    25801 tcatccgcaa ccgaccgcgc cagaagtaca aaaaccgact ctacactaaa aacaaaaaac
    25861 gtaaggagag tgttatgagt gagatatccc ggcaagagtt tcagcgtcgc cgtcaggccc
    25921 tggtggagca aatgcaaccc ggcagcgccg cgctgatttt tgctgcacca gaagtaacac
    25981 gtagcgccga cagcgaatac ccctatcgtc agaacagtga cttctggtac ttcaccggct
    26041 ttaacgaacc ggaagcggtg ctggtgctga ttaaaagcga tgacactcat aaccacagcg
    26101 ttctgtttaa ccgcgttcgc gacctgacgg cggagatctg gtttggccgt cgcttaggcc
    26161 aggatgccgc gccagagaaa ctgggcgttg accgcgcact ggcattcagc gaaatcaatc
    26221 agcaacttta tcaactactt aacggcctgg atgtggttta ccatgcccag ggcgaatatg
    26281 catatgctga tgaaatcgtg aacagtgcgc tggaaaaact gcgtaaaggt tcgcggcaaa
    26341 atctcaccgc accggcaacg atgatcgact ggcgtcctgt tgttcatgaa atgcgcctgt
    26401 tcaaatcgcc agaagagatt gccgtactcc gccgcgcggg agaaatcacc gccatggcac
    26461 atacacgggc gatggaaaaa tgccgtccgg gaatgttcga gtaccatctg gaaggcgaaa
    26521 ttcaccacga atttaaccgc cacggtgcgc gctatccttc ctataacacc attgtaggca
    26581 gcggtgaaaa cggctgcatt ctgcactaca ccgaaaacga gagtgaactg cgcgacggtg
    26641 acctggtgtt gattgacgcg ggttgtgaat acaaaggtta cgcgggcgat attacccgca
    26701 ccttcccggt caacggcaaa ttcacccagg cccagcgtga aatctacgac attgtgctgg
    26761 agtctctcga aaccagcctg cgcctgtatc gtccgggaac ttccattctg gaagtcactg
    26821 gtgaagtggt gcgcatcatg gttagcggcc tggtaaaact cggcatcctg aaaggtaatg
    26881 ttgatgaact gatcgctcag aacgcccatc gtcctttctt tatgcatggc cttagccact
    26941 ggttaggact ggatgtccat gacgttggcg tttatggtca ggatcgctcg cgcattctgg
    27001 aaccggggat ggtactgacc gtagagcctg gtctgtatat cgcgccggat gcggatgtgc
    27061 cagaacaata tcgcggtatc ggcattcgta ttgaagacga cattgtgatt accgaaaccg
    27121 gcaacgaaaa cctcaccgcc agcgtggtga aaaagccgga agaaatcgaa gcgttgatgg
    27181 ctgctgcgag aaagcaatga gcgtaatcat cgtcggtggc ggcatggcgg gcgcgacgct
    27241 ggcgctggct atttcccggt taagtcacgg ggcgctgccg gtacatttga ttgaagcgac
    27301 tgcgccagag tcacatgctc atccgggctt tgatggacga gcgatagcgc tggcggcggg
    27361 tacctgtcag caactggcgc gcatcggcgt ctggcaatct ctggctgatt gcgcaaccgc
    27421 catcactacc gtgcatgtca gcgatcgtgg tcatgctgga ttcgtcaccc tcgccgcaga
    27481 agattaccaa ctggcggcgc tggggcaggt tgtcgaattg cataatgtcg ggcaacggct
    27541 gtttgcattg ctgcgaaaag cacctggcgt aatgctgcat tgccccgatc gcgtagctaa
    27601 cgttgcccgt actcagagtc atgttgaagt gacgctggag agcggcgaga tgctgacggg
    27661 ccgcgtgctg atagccgctg atggcaccca ttcagcgtta gccaccgcgt gcggcgttga
    27721 ttggcagcag gagccttacg aacaactggc agtgattgcc aacgttgcta cttctgttgc
    27781 gcacgaaggc cgcgcttttg aacgcttcac gcaacatggc ccgctggcga tgttgccgat
    27841 gtctgacgga cgctgttcgc tggtctggtg tcatccactg gaacggcgcg aagaggtgct
    27901 gtcgtggagc gacgagaagt tttgccgtga actccagtct gcctttggct ggcgactggg
    27961 gcaaattacc cacgccggta aacgcagtgc ttatccgctg gcattaaccc gcgccgccag
    28021 accgattacc catcgcactg tgctggtggg caatgcggcg caaactctgc acccgattgc
    28081 cgggcaaggg tttaacctcg gtatgcgaga tgtcatgagc cttgcggaaa ccctgactca
    28141 ggcgcatgag cgcggagaag acatcggtga ttacggcata ttgtgccgct atcagcagcg
    28201 tcgacagagc gatcgcgaag caaccatcgg cgtcacggac agcctggtac atctttttgc
    28261 caaccgttgg gcaccgctgg ttgtcgggcg caacatcgga ctgatgacga tggaattatt
    28321 caccccggca cgcgatgtgc tggcgcagcg caccctcggt tgggtggcgc gttgaaggcg
    28381 gttttaagga ataaacaatg caaagtgttg atgtagccat tgttggcggc ggcatggtgg
    28441 ggctggcggt tgcctgcggc ttacagggga gcggcttacg cgttgccgta ctggagcagc
    28501 gcgtaccgga acctctggcg gtagatgcac taccacaact gcgcgtttcg gctatcaatg
    28561 ccgccagcga aaaattactc acccgtcttg gcgtctggca ggacattctc gctcgtaggg
    28621 ccagttgtta tcacggcatg gaagtgtggg acaaagacag ttttggtcac atctcgtttg
    28681 acgatcaaag catgggctat agccatcttg ggcacatcgt tgaaaactca gtgattcact
    28741 acgcgctgtg gaacaaagcg cagcagtcgt cagatattac tctgttggcc cccacagaat
    28801 tacagcaggt cgcctgggga gaaaatgaaa ccttcctgac gctgaaagat ggcagcatgt
    28861 taacggcgcg tctggtgatt ggcgcggacg gtgctaattc ctggttgcgc aacaaagcag
    28921 atattccgct gactttctgg gattatcagc atcacgcgct ggtagcgacc attcgcacgg
    28981 aagaaccgca tgatgcggtg gcgcggcagg ttttccatgg cgaaggcatt ctggcctttt
    29041 taccgcttag cgatccgcat ctttgctcga ttgtctggtc actgtcgcca gaggaagcgc
    29101 agcggatgca gcaggcaagt gaagacgaat ttaatcgcgc gttaaatatc gcttttgata
    29161 atcgccttgg cttatgcaag gttgagagcg cgcgtcaggt gttcccgctg acggggcgtt
    29221 atgcgcgcca gtttgccgca caccgtctgg cgctggtggg cgacgccgcg cataccattc
    29281 acccactggc ggggcagggc gttaatctcg gatttatgga cgccgcagag ctgattgctg
    29341 aactgaaacg gttgcatcgt cagggtaaag acatcgggca gtacatttac ctgcgccgct
    29401 atgaacgtag ccgcaagcac agcgcggcgc tgatgctggc tggtatgcag ggttttcgcg
    29461 atctcttttc cggcgctaat ccggcgaaaa aactgctgcg tgatattggt ttgaaactgg
    29521 ccgacacgct tcctggcgtt aagccgcaac ttatccgcca ggcaatggga ttaaacgatt
    29581 tgcctgaatg gctgcgttaa aaatttctcc tctgttgttt atttgatacc catcacactt
    29641 tcatctcccg gttttttcgc cgggagattt tcctcatttg aaataaacta atttcacctc
    29701 cgttttcgca ttatattttc taataccatg attttttgat ttagtgtttt ttgacatttt
    29761 tttagctctt aatattgtct tattcaaatt gactttctca tcacatcatc tttgtataga
    29821 aattggtgta ttttttggtt ttttattctg tcgcgatttt tgcatttttt aaccataagc
    29881 taatgtgatg atcaatttta ccttatggtt aacagtctgt ttcggtggta agttcaggca
    29941 aaagagaacg attgcgttgg ggaccgggag tggctccgat gctgggtttt gtggtgataa
    30001 tttcaccatg aaaaagttgt cagccccgct tattcaatga ggacaagatg gcacaacaga
    30061 ctcctttgta cgaacaacac acgctttgcg gcgctcgcat ggtggatttc cacggctgga
    30121 tgatgccgct gcattacggt tcgcaaatcg acgaacatca tgcggtacgt accgatgccg
    30181 gaatgtttga tgtgtcgcat atgaccatcg tcgatctccg cggcagccgc acccgggagt
    30241 ttctgcgtta tctgctggcg aacgatgtgg cgaagctcac caaaagcggc aaagcccttt
    30301 actcggggat gttgaatgcc tctggcggtg tgatagatga tctcatcgtc tactacttta
    30361 ctgaagattt cttccgcctc gttgttaact ccgccacccg cgaaaaagac ctctcctgga
    30421 ttacccaaca cgctgaacct ttcggcatcg aaattaccgt tcgtgatgac ctttccatga
    30481 ttgccgtgca agggccgaat gctcaggcaa aagctgccac actgtttaat gatgctcagc
    30541 gtcaggcggt ggaagggatg aaaccgttct ttggcgtgca ggtgggcgat ctgtttattg
    30601 ccaccactgg ttataccggt gaagcgggct atgaaattgc gctgcccaat gaaaaagcgg
    30661 ccgatttctg gcgtgcgctg gtggaagcgg gcgttaagcc ctgcggtctg ggggcgcgtg
    30721 acacgctgcg tctggaagcg ggcatgaatc tttatggtca ggagatggac gaaactattt
    30781 ctcctttagc cgccaacatg ggctggacta tcgcctggga accgacagat cgtgacttta
    30841 tcggtcgtga agccctggaa gtgcagcgtg agcatggtac agaaaaactg gttggtctgg
    30901 tgatgaccga aaaaggcgtg ctgcgtaatg aactgccggt acgctttacc gatgcgcagg
    30961 gcaaccagca tgaaggcatt atcaccagcg gtactttctc cccgacgctg ggttacagca
    31021 ttgcgctggc gcgcgtgccg gaaggtattg gcgaaacggc gattgtgcaa attcgcaacc
    31081 gtgaaatgcc ggttaaagtg acaaaacctg tttttgtgcg taacggcaaa gccgtcgcgt
    31141 gatttacttt tttggagatt gattgatgag caacgtacca gcagaactga aatacagcaa
    31201 agaacacgaa tggctgcgta aagaagccga cggcacttac accgttggta ttaccgaaca
    31261 tgctcaggag ctgttaggcg atatggtgtt tgttgacctg ccggaagtgg gcgcaacggt
    31321 tagcgcgggc gatgactgcg cggttgccga atcggtaaaa gcggcgtcag acatttatgc
    31381 gccagtaagc ggtgaaatcg tggcggtaaa cgacgcactg agcgattccc cggaactggt
    31441 gaacagcgaa ccgtatgcag gcggctggat ctttaaaatc aaagccagcg atgaaagcga
    31501 actggaatca ctgctggatg cgaccgcata cgaagcattg ttagaagatg agtaacggct
    31561 ttattcctct tctgcgggag aggatcaggg tgaggaaaat ttatgcctca ccctcactct
    31621 cttcgtaagg agagaggttc acaattcact gcacgtttca ggaaccatcg ctcatgacac
    31681 agacgttaag ccagcttgaa aacagcggcg cttttattga acgccatatc ggaccggacg
    31741 ccgcgcaaca gcaagaaatg ctgaatgccg ttggtgcaca atcgttaaac gcgctgaccg
    31801 gccagattgt gccgaaagat attcagcttg cgacaccacc gcaggttggc gcaccggcga
    31861 ccgaatacgc cgcactggca gaactcaagg ctattgccag tcgcaataaa cgcttcacgt
    31921 cttacatcgg catgggttac accgccgtgc agctaccgcc ggttatcctg cgtaacatgc
    31981 tggaaaatcc gggctggtat accgcgtaca ctccgtatca gcctgaagtc tcccagggcc
    32041 gccttgaagc actgctcaac ttccagcagg taacgctgga tttgactgga ctggatatgg
    32101 cctctgcttc tcttctggac gaggccaccg ctgccgccga agcaatggcg atggcgaaac
    32161 gcgtcagcaa actgaaaaat gccaaccgct tcttcgtggc ttccaatgtg catccgcaaa
    32221 cgctggatgt ggtccgtact cgtgctgaaa cctttggttt tgaagtgatt gtcgatgacg
    32281 cgcaaaaagt gctcgaccat caggacgtct tcggcgtgct gttacagcag gtaggcacta
    32341 ccggtgaaat tcacgactac actgcgctta ttagcgaact gaaatcacgc aaaattgtgg
    32401 tcagcgttgc cgccgatatt atggcgctgg tgctgttaac tgcgccgggt aaacagggcg
    32461 cggatattgt ttttggttcg gcgcaacgct tcggcgtgcc gatgggctac ggtggcccac
    32521 acgcggcatt ctttgcggcg aaagatgaat acaaacgctc aatgccgggc cgtattatcg
    32581 gtgtatcgaa agatgcagct ggcaataccg cgctgcgcat ggcgatgcag actcgcgagc
    32641 aacatattcg tcgtgagaaa gcgaactcca acatttgtac ttcccaggta ctgctggcaa
    32701 acatcgccag cctgtatgcc gtttatcacg gcccggttgg cctgaaacgt atcgctaacc
    32761 gcattcaccg tctgaccgat attctggcgg cgggcctgca acaaaaaggt ctgaaactgc
    32821 gccatgcgca ctatttcgac accttgtgtg tggaagtggc cgacaaagcg ggcgtactga
    32881 cgcgtgccga agcggctgaa atcaacctgc gtagcgatat cctgaacgcc gtggggatca
    32941 cccttgatga aaccaccacg cgtgaaaacg taatgcagct tttcagtgtg ctgctgggcg
    33001 ataaccacgg gctggagatc gacacgctgg acaaagacgt ggctcacgac agccgctcta
    33061 tccagcctgc gatgctgcgc gacgacgaaa tcctcaccca tccggtgttt aatcgctatc
    33121 acagcgaaac cgaaatgatg cgctatatgc actcgctgga gcgtaaagat ctggcgctga
    33181 atcaggcgat gatcccgctg ggttcctgca ccatgaaact gaacgccgcc gccgagatga
    33241 tcccaatcac ttggacggaa tttgccgaac tgcacccgtt ttgcccgccg gaacaggctg
    33301 aaggttatca gcagatgatt gcgcagctgg ctgactggct ggtgaaactg accggttacg
    33361 acgccgtttg tatgcagccg aactccggcg cacagggcga atacgcgggc ctgctggcga
    33421 ttcgtcatta tcatgaaagc cgcaacgaag ggcatcgcga tatctgcctg atcccggctt
    33481 ctgcgcacgg aactaacccc gcttctgcac atatggcagg aatgcaggtg gtggttgtgg
    33541 cgtgtgataa aaacggcaac atcgatctga ctgatctgcg cgcgaaagcg gaacaggcgg
    33601 gcgataacct ctcctgtatc atggtgactt atccttctac ccacggcgtg tatgaagaaa
    33661 cgatccgtga agtgtgtgaa gtcgtgcatc agttcggcgg tcaggtttac cttgatggcg
    33721 cgaacatgaa cgcccaggtt ggcatcacct cgccgggctt tattggcgcg gacgtttcgc
    33781 acctcaacct gcataaaact ttctgcattc cgcacggcgg tggtggtccg ggtatgggac
    33841 cgatcggtgt gaaagcgcat ctggcaccgt ttgtaccggg tcatagcgtg gtgcaaatcg
    33901 aaggcatgtt aacccgtcag ggcgcagttt ctgcggcacc gttcggtagc gcctctatcc
    33961 tgccaatcag ctggatgtac atccgcatga tgggcgcaga agggctgaaa aaagcaagcc
    34021 aggtggcaat cctcaacgcc aactatattg ccagccgcct gcaggatgcc ttcccggtgc
    34081 tgtataccgg tcgcgacggt cgcgtggcgc acgaatgtat tctcgatatt cgcccgctga
    34141 aagaagaaac cggcatcagc gagctggaca ttgccaagcg cctgatcgac tacggtttcc
    34201 acgcgccgac gatgtcgttc ccggtggcgg gtacgctgat ggttgaaccg actgaatctg
    34261 aaagcaaagt ggaactggat cgctttatcg acgcgatgct ggctatccgc gcagaaattg
    34321 accaggtgaa agccggtgtc tggccgctgg aagataaccc gctggtgaac gcgccgcaca
    34381 ttcagagcga actggtcgcc gagtgggcgc atccgtacag ccgtgaagtt gcggtattcc
    34441 cggcaggcgt ggcagacaaa tactggccga cggtgaaacg tctggatgat gtttacggcg
    34501 accgtaacct gttctgctcc tgcgtaccga ttagcgaata ccagtaattc actgattcga
    34561 gtattttcta aaggcgcttc ggcgcctttt tagtcagatg acaaagtaca aaagtgctca
    34621 gacagtcccc tcgccccttt ggggagaggg ttagggtgag gggaacaggc cggcactggc
    34681 gcgaatattt accctcaccc cgaccctctc cctgaaaggg cgagggggaa aagcgtgcca
    34741 acattgaaga ttgagccagt ttgttagcaa tctcaaagat acgtcaacga attaattttt
    34801 ctcggaaaaa caaatggcta tagcacttgt gaccggtggc agtcgcggca tcgggcgggc
    34861 aactgcatta ctgttggcgc aagaagggta tacggtggcg gttaattatc agcaaaacct
    34921 ccacgcggcg caggaagtga tgaacttaat aatgcaagcc ggtggcaaag cattcgtgct
    34981 ccaggcggat atcagcgacg aaaaccaggt cgttgcgatg tttacagcaa tcgatcagca
    35041 cgatgaaccg ctagcagcgc tggtcaataa cgccgggatc ttgtttaccc agtgcaccgt
    35101 tgaaaacctt accgcagagc gaatcaaccg agtactttcc accaacgtga cgggatattt
    35161 tctctgctgc cgcgaggcgg taaaacgcat ggcgcttaaa aatggtggca gtggcggcgc
    35221 tatcgtcaat gtctcttcgg tggcctcacg gttgggttcg ccaggggaat atgttgatta
    35281 cgcggcatcg aaagggacga ttgattcgtt aaccaccgga ctatcgctgg aagtcgccgc
    35341 gcaggggatc cgcgttaact gcgtgcggcc agggtttatt tataccgaaa tgcacgccag
    35401 cggcggcgag cctggacgcg tcgatcgcgt taagtcgaac atccccatgc agcgtggtgg
    35461 acaggcagaa gaggtcgcgc aggccattgt ctggctacta agtgataaag cctcttacgt
    35521 cacgggaagt tttatcgatt tggcgggcgg gaaataaaac agggaagttg tctgaccgga
    35581 tgcaacaagt attgcatccg gtacttcatc gacttaaagc ttctcgccgt tgctggcaat
    35641 cacctctttg taccagttaa agctcttctt acgtgaacgc gacatatcgc cagtaccgtc
    35701 gtcatgttta ttcacataga taaagccgta gcgtttgctg tactgcccgg tagtgaatga
    35761 cacgcagtcg atgcagcccc acggtgtgta gcccatcaga tccacaccat cgtaagtcac
    35821 tgcttttttc atctcttcaa tatgggcgcg caggtagtca atgcggtagt cgtcgttgat
    35881 gctgccatct tcttccactt tgtcgtaagc gccaaaaccg ttttcgacaa taaacagcgg
    35941 cctctgataa cgctcataca gttcgcaaag ggcatagcgc agacctactg gatcaatctg
    36001 ccagccccag tcagacgctt taacatacgg gttcggtacg ctgccttcaa aaccagagat
    36061 cgcatcgccg gtgccgcctt cggccttcac tgcgttggtc atgtaatagc tgaaaccaag
    36121 ataatcgcag gtgccttcgc gcagtacatc gagatcgccg tcttccattt tgatattaaa
    36181 tccgcgacgt tcccactcgt tcaatacata ggacgggtaa tagccgcata gctgcacatc
    36241 ggtaaagacg tagcgttcgc gcatcgactc ctgagcgaac atcacatcgt ccgggttaca
    36301 tgagtaggga tagagcggca ccatcgccag catacagccg actttcatct ccgggttaat
    36361 gcgacgcgcg gctttcactg ccagggcgct ggcgacaaac tggtgatgca gcacctggta
    36421 catcgtctct tccgggttgt catgctcggt atacaccacg ccggagcagc agtaaccaaa
    36481 cagcggcgca cgccagttac gctggttgtt tatttcgttg aaggtcatcc agtatttgac
    36541 tttatgcttg tagcgttcga ataccacttc cgcgaaacgg acaaagaaat caacgaattt
    36601 acggttggtc cagctaccgt attgctgcac cagatgcagc ggcatttcaa agtgggagag
    36661 ggttatcacc ggttcgatgt tgtatttcag cagttcatcg aacagagagt cgtaaaactt
    36721 cagcccttct tcgtttggct gagcttcatc accttttgga aaaatacgcg tccaggcgat
    36781 ggaagtccga aaacatttga agcccatttc ggcaaatagc ttgatgtctt ccttgtagtg
    36841 accataaaaa tcaacggctt catggtttgg atagtatttt cctggcaaga cttctttggt
    36901 gatttcgcgc ggcacgccgt gtgcgccaca ggtcagaacg tcacaaatgc tcggtccttt
    36961 gccgcctttg ttccagccgc cttcgacctg atgagcggca acggcgccac cccataagaa
    37021 atctttcggt aaggtgagtt ttttcactat cataggctcc agatcagtca attcttacag
    37081 gaaatagtac catgcagcca aacgacatca cttttttcaa cgtttccagg atgacattct
    37141 ggctgggcgt aaaaccatca ccatccgcga cgagtctgaa tcgcacttca aaacgggtga
    37201 tgtgcttcgt gtcggacgtt ttgaagatga cggttatttt tgcacgattg aagtcaccgc
    37261 aacctcaacc gtaacgctgg atacactgac ggaaaaacat gcagagcagg aaaatatgac
    37321 cctgcctgaa ctgaaaaagg tcattgctga catctatccc ggtcagacac aattttatat
    37381 gattgaattt aaatgtcttt aaattgaact cacgcctgtt agttgaaatt tcgcatttaa
    37441 tttcatgatt tttaggagat tagtctcaaa tcagaattta ttttagctaa caggtgttca
    37501 ctggaactat tctcagttac gctggagagg tatacatgtg cgtgtacgtt tctctggagt
    37561 aagttatggt tcagaagccc ctcattaagc agggatattc gctggcagag gaaattgcca
    37621 acagcgtcag tcacggcatt gggctggtgt ttggtatcgt tgggctggtg ttgctactgg
    37681 ttcaggcggt ggatctcaat gccagcgcca cagcgataac cagttacagc ctctatggcg
    37741 gcagtatgat cctgctgttc ctcgcttcga cgctctatca cgccattcct catcaacggg
    37801 caaaaatgtg gctgaagaaa tttgaccatt gcgctattta cctgttgatt gccggaacct
    37861 acacgccgtt tttgctggtg gggctggatt ctccgttagc gcgcgggttg atgattgtta
    37921 tctggagcct agcattgctg gggattctgt tcaaactgac catcgcgcac cgattcaaaa
    37981 ttttatctct ggtgacttat ctggcgatgg gctggctgtc gctggtggta atttatgaaa
    38041 tggcagttaa gctcgcggcg ggcagcgtta ccttactggc ggtaggcggc gtggtttatt
    38101 cgctcggggt gattttctac gtctgcaaac gcattccata caaccatgcc atctggcacg
    38161 gcttcgtgct cggcggtagt gtgtgccact ttctggcgat ctatttgtat attgggcagg
    38221 cgtaatgggt atgcctttgt aggcctgata agacgcgtca gcgtcgcatc aggcatcgga
    38281 gcgcaaccgc cggatgcggc gtgaacgcct tatccgatct acgggcctcg tccctgttgt
    38341 gataaaggca tcaaatccca gacatgataa gacgcgtcag cgtcgcatca ggcgataaat
    38401 gcctgatgcg atacagagat ttactcttcg agcgaatacg gcagcggctc gatatgcagt
    38461 gtattcgcat cgtcgcgtac gcggaagatg ctgtccggtt ccatatcgtt attcatgacg
    38521 acctgtacca cgacctgacc atcttccagt tttaccgcag ccagcaccgt accggtacga
    38581 cgccagttct cgcccatttt cagctctaag tcttcaccag cttccggcag tcggctggca
    38641 ctacctgcca gcaaccagag cgcgcgtttg ttggcaccac ggaattttgc tcgcgccacc
    38701 atctcttgtc cggtataaca gcctttctta aagctgatac cgcccagcgc ctggagattg
    38761 gtcgcctgtg ggataaactg cccgctgttg gcggcatcaa tcaccgggaa accagcttca
    38821 atgtttaatg ccagccactg ttggctattg ttcagttccg cttcaccgcg cagtttatcg
    38881 gtcagcatat tagcagtagc ttcatcggtt acgatcagga aacgttctgc cgggtgttca
    38941 aaccatagca aagtggtcgc gccttctttg actacctgtt tttctttcga aggcagttcg
    39001 ctaaagagat ttgccagcgc ggcgcgcgcc tgaaaaccgg caacaccaag cagcacacgc
    39061 tcgtcgtctg gcgcgatggt cactttagag aataccgcat attttttcag ttcagtcagc
    39121 tgcggttcac gcacgctgcg ccgttcaatc catgcaaagc catcgccgtc gcggaacaga
    39181 cgtaaattgc tccacatttt acctttggcg tcgcaatggg cggcgagcag gtgctgatct
    39241 tctgccatct ggctgacatc tgctgtcacc tgaccctgca tatatttttc gctgtccgcg
    39301 ccagtaatgg tggcaagcgc ccagtcatca agcgtcatca gcgtcagcgg taaacgggca
    39361 gaagccgtag gctgacgtgg aggaaaaggt gtaaaagcca taatagagtc ctgagttgct
    39421 taacggcgta ttaatgacta atggtaaaag agctatgagg caatgcaagt gctttcacta
    39481 cgcgatctgt gccttctgcg aggcgactgc caaaaaaatg aagcccttga attgttgtga
    39541 attaatttcg cgatcgcgat ggtgtttact gatattcccg taaaagaagt cgaaaaacac
    39601 gttacaatag ccgggtactg gattttcgtg agacaggaag aagatggaca ttaacaacaa
    39661 agcccgaatt cattgggcat gccgccgtgg tatgcgcgaa ctcgatattt caatcatgcc
    39721 gtttttcgaa catgagtacg acagcttaag cgatgacgaa aaacgcatct ttattcgtct
    39781 gctggaatgt gacgatccgg acctgtttaa ctggctgatg aatcacggta aaccggccga
    39841 tgcagaactg gaaataatgg tccgactcat ccagacacgg aaccgggaac gtggtcctgt
    39901 ggcaatctga tttgcgcgtc tcctggcgcg cacagtggct ttccttgctg attcatgggc
    39961 tggttgccgc tgttatttta ctcatgccct ggccgctcag ttacaccccg ttatggatgg
    40021 tgttactttc gctggtggta tttgattgcg ttcgcagcca gcggtgtatt aatgctcgcc
    40081 agggggaaat tcgcttgttg atggacgggc gtttgcgttg gcaagggcag gagtggagca
    40141 tcgtcaaagc accgtggatg attaagagcg gcatgatgct gcgtttacgt tctgatggcg
    40201 gtaaacggca acatttatgg ctggcagccg acagcatgga cgaagccgaa tggcgggatt
    40261 tacggcggat tttgttgcaa caagagacgc aaagataaag atgacggcaa agcatatgcg
    40321 ctaagccgtc agtgagatca ggcgtaatgc tctgccattt cgttgaggat ttgctcgcac
    40381 cagctctgaa tacgctcgtc gctaaggtca tactggttag tttcatccag cgccagaccc
    40441 acgaacagtt gcccgtcagc aatcaccggt ttcgggctgg taaattcata tccttccgtt
    40501 ggccagtagc cgacgaactt cacgcctttg gtcgagagtt tgtcatgcag cataccgagc
    40561 gcatcgagga accactcgcc gtatcccagt tgatcgccaa gcccatacag cgcaataatt
    40621 ttaccttcaa ggttcaggtc gtcgagctga tcccagacgg cttcccagtc ttcctggatt
    40681 tcaccaaaat cccaggtcgg gatacccaga atgagcacat cgtactgctc cattaatttc
    40741 ggggagtcgt ccttgaggtt atgtaaggtc accagttctg ggccgataat atcgcggatt
    40801 ttttctgccg ccatttcggt gtaacaggtg ctggaaccgt aaaaaagacc catattcata
    40861 acgtaagctt ctcgattctg ctgtggcttt tgcagctagt gtatcagaat cgctttagtc
    40921 acggcacaat aaggcataat ggatcaaatg agtgaccata aggggcgcaa gtgaaacagg
    40981 agctggcacg catcgagcag tttcttgatg ctctgtggct ggaaaaaaat ctggctgaaa
    41041 atacgttgaa cgcttaccgt cgcgatctgt caatgatggt ggagtggttg catcaccgcg
    41101 ggttgacgct ggcgacggcg caaagtgacg atttgcaggc attactggca gaacggctgg
    41161 agggcgggta taaagccacc agctcagcgc gtttgctgag tgcagtgcgc cgattgttcc
    41221 agtatcttta tcgcgaaaag tttcgtgaag acgatcccag tgcgcatctc gcttcaccga
    41281 aattgcccca gcgtttgcca aaagatttaa gcgaagcgca ggtcgaacgt ttattacagg
    41341 cgccattaat tgatcagcca ctggagctac gcgataaagc catgcttgaa gtgttgtatg
    41401 ctaccgggct gcgtgtctct gaactggtcg ggctgacaat gagtgatatc agcctgcgtc
    41461 agggcgtagt gcgggtcatt ggtaaaggca acaaagagcg cctggtgccg ttaggggaag
    41521 aggcggttta ctggctggaa acctatctgg aacatgggcg tccgtggctg ctgaatggtg
    41581 tgtcaattga cgtgttgttt cccagccagc gtgcgcagca gatgacgcga cagaccttct
    41641 ggcaccgcat taaacattat gccgtgctgg cgggtatcga cagcgaaaag ctgtcaccgc
    41701 atgtgttgcg tcacgctttt gccactcatt tattaaatca tggtgcggat ttacgcgtgg
    41761 tgcagatgct actgggccac agcgatcttt ccaccacgca aatttatacg catgtcgcta
    41821 ccgagcgtct gcggcaactt catcaacagc atcacccgcg ggcgtgatgt ctgaaaagaa
    41881 cgggaagatt tatgaagaaa ggttttatgt tgtttacttt gttagcggcg ttttcaggct
    41941 ttgctcaggc tgatgacgcg gcaattcaac aaacgttagc caaaatgggc atcaaaagca
    42001 gcgatattca gcccgcgccc gtagctggca tgaagacagt tctgactaac agcggtgtgt
    42061 tgtacatcac cgatgatggt aaacatatca ttcaggggcc aatgtatgac gttagtggca
    42121 cggctccggt caatgtcacc aataagatgc tgttaaagca gttgaatgcg cttgaaaaag
    42181 agatgatcgt ttataaagcg ccgcaggaaa aacacgtcat caccgtgttt actgatatta
    42241 cctgtggtta ctgccacaaa ctgcatgagc aaatggcaga ctataacgcg ctggggatca
    42301 ccgtgcgtta tcttgctttc ccgcgccagg ggctggacag cgatgcagag aaagaaatga
    42361 aagctatctg gtgtgcgaaa gataaaaaca aagcgtttga tgatgtgatg gcaggtaaaa
    42421 gcgtcgcacc agccagctgc gacgtggata ttgccgacca ttacgcactt ggcgtccagc
    42481 ttggcgttag cggtactccg gcagttgtgc tgagcaatgg cacacttgtt ccgggttacc
    42541 agccgccgaa agacatgaaa gaattcctcg acgaacacca aaaaatgacc agcggtaaat
    42601 aattcgcgtg aaacaacaga tacaacttcg tcgccgtgaa gtcgatgaaa cggcagactt
    42661 gcccgctgaa ttgcctccct tgctgcgccg tttatacgcc agccggggag tacgcagtgc
    42721 gcaagaactg gaacgcagtg ttaaaggtat gttgccctgg cagcaactaa gcggtgtcga
    42781 aaaggccgtt gagatccttt acaacgcttt tcgcgaagga acgcggatta ttgtggtcgg
    42841 tgatttcgac gccgacggcg cgaccagcac ggctctaagc gtgctggcga tgcgctcgct
    42901 tggttgcagc aatatcgact acctggtacc aaaccgtttc gaagacggtt acggcttaag
    42961 cccggaagtg gtcgatcagg cccatgcccg tggcgcgcag ttaattgtca cggtggataa
    43021 cggtatttcc tcccatgcgg gagttgagca cgctcgctcg ttgggcatcc cggttattgt
    43081 taccgatcac catttgccgg gcgacacatt acccgcagcg gaagcgatca ttaaccctaa
    43141 cttgcgcgac tgtaatttcc cgtcgaaatc actggcaggc gtgggtgtgg cgttttatct
    43201 gatgctggcg ctgcgcacct ttttgcgcga tcagggctgg tttgatgagc gcggcatcgc
    43261 aattcctaac ctggcagaac tgctggatct ggtagcactg gggacagtag cggacgtcgt
    43321 gccgctggac gctaataatc gcattctgac ctggcagggg atgagtcgca tccgtgccgg
    43381 aaagtgccgt ccagggatta aagcgctgct ggaagtggca aatcgcgatg cacaaaaact
    43441 agccgccagc gatttaggtt ttgcgctggg accgcgtctc aatgctgccg ggcgactgga
    43501 tgatatgtcc gttggtgtgg cgctgttgct gtgcgacaac atcggcgaag cgcgcgtgct
    43561 ggcaaatgaa ctcgatgcgc taaaccagac gcgaaaagag atcgaacaag gaatgcaggt
    43621 tgaagccctg accctgtgcg agaaactgga gcgcagccgt gacacgctac ccggcgggct
    43681 ggcaatgtat caccccgaat ggcatcaggg cgttgtcggt attctggctt cgcgtatcaa
    43741 agagcgtttt caccgtccgg ttatcgcctt tgcgccagca ggtgacggta cgctgaaagg
    43801 ttccggtcgc tccattcagg ggctgcatat gcgtgatgcg ctggagcgat tagacacact
    43861 ctaccccggc atgatgctca agtttggcgg tcatgcgatg gcggcgggtt tgtcgctgga
    43921 agaggataaa ttcgaactct ttcaacaacg gtttggcgaa ctggttactg agtggctggc
    43981 cccttcgcta ttgcaaggcg aagtggtgtc agacggtccg ttaagcccgg ccgaaatgac
    44041 catggaagtg gcgcagctgc tgcgcgatgc tggcccgtgg gggcagatgt tcccggagcc
    44101 gctgtttgac ggtcatttcc gtctgctgca acagcggctg gtgggcgaac gtcatttgaa
    44161 ggtgatggtc gaaccggtcg gcggcggtcc actgctggat ggtattgctt ttaatgtcga
    44221 taccgctctc tggccggata acggcgtgcg cgaagtgcaa ctggcttata agctcgatat
    44281 caacgagttt cgcggcaacc gcagcctgca aattatcatc gacaatatct ggccaattta
    44341 gcgtcatctt ctctataaaa aagagcgtgg attgggtaca atcccgctct tatcaccgca
    44401 ttttgactag ctcaataaaa gaaatcagac catgtttgaa attaatccgg taaataatcg
    44461 cattcaggac ctcacggaac gctccgacgt tcttaggggg tatctttgac tacgacgcca
    44521 agaaagagcg tctggaagaa gtaaacgccg agctggaaca gccggatgtc tggaacgaac
    44581 ccgaacgcgc acaggcgctg ggtaaagagc gttcctccct cgaagccgtt gtcgacaccc
    44641 tcgaccaaat gaaacagggg ctggaagatg tttctggtct gctggaactg gctgtagaag
    44701 ctgacgacga agaaaccttt aacgaagccg ttgctgaact cgatgccctg gaagaaaaac
    44761 tggcgcagct tgagttccgc cgtatgttct ctggcgaata tgacagcgcc gactgttacc
    44821 tcgatattca ggcggggtct ggcggtacgg aagcacagga ctgggcgagc atgcttgagc
    44881 gtatgtatct gcgctgggca gaatcgcgtg gtttcaaaac tgaaatcatc gaagagtcgg
    44941 aaggtgaagt ggcgggtatt aaatccgtga cgatcaaaat ctccggcgat tacgcttacg
    45001 gctggctgcg tacagaaact ggcgttcacc gcctggtgcg taaaagcccg tttgactccg
    45061 gcggtcgtcg ccacacgtcg ttcagctctg cgtttgttta tccggaagtt gatgatgata
    45121 ttgatatcga aatcaacccg gcggatctgc gcattgacgt ttatcgcgcg tccggcgcgg
    45181 gcggtcagca cgttaaccgt accgaatctg cggtgcgtat tacccacatc ccgaccggga
    45241 tcgtgaccca gtgccagaac gaccgttccc agcacaagaa caaagatcag gccatgaagc
    45301 agatgaaagc gaagctttat gaactggaga tgcagaagaa aaatgccgag aaacaggcga
    45361 tggaagataa caaatctgac atcggctggg gcagccagat tcgttcttat gtccttgatg
    45421 actcccgcat taaagatctg cgcaccgggg tagaaacccg caacacgcag gccgtgctgg
    45481 acggcagcct ggatcaattt atcgaagcaa gtttgaaagc agggttatga ggaaccaaca
    45541 tgtctgaaca acacgcacag ggcgctgacg cggtagtcga tcttaacaat gaactgaaaa
    45601 cgcgtcgtga gaagctggcg aacctgcgtg agcagggaat tgccttcccg aacgatttcc
    45661 gtcgcgatca tacctctgac caattgcacg cagaattcga cggtaaagag aacgaagaac
    45721 tggaagcgct gaacatcgaa gtcgccgttg ctggccgcat gatgacccgc cgtattatgg
    45781 gtaaagcgtc tttcgttacc ctgcaggatg tgggtggccg cattcagctg tacgttgccc
    45841 gtgacgatct gccggaaggc gtttacaacg agcagttcaa aaaatgggac ctcggcgaca
    45901 tcctcggcgc gaaaggtaaa ctgttcaaaa ccaaaaccgg cgaactgtct atccactgta
    45961 ccgagctgcg tctgctgacc aaagcactgc gtccgctgcc ggataaattc cacggcttgc
    46021 aggatcagga agcgcgctat cgtcagcgtt atctggatct catctctaac gatgaatccc
    46081 gtaacacctt taaagtacgc tcgcagatcc tctctggtat tcgccagttc atggtgaacc
    46141 gcggctttat ggaagttgaa acgccgatga tgcaggtgat cccaggcggt gccgctgcgc
    46201 gtccgtttat cacccaccat aacgcgctgg atctcgacat gtacctgcgt atcgcgccgg
    46261 aactgtacct caagcgtctg gtggtcggtg gcttcgagcg tgtattcgaa atcaaccgta
    46321 acttccgtaa cgaaggtatt tccgtacgtc ataacccaga gttcaccatg atggaactct
    46381 acatggctta cgcagattac aaagatctga tcgagctgac cgaatcgctg ttccgtactc
    46441 tggcacagga tattctcggt aagacggaag tgacctacgg cgacgtgacg ctggacttcg
    46501 gtaaaccgtt cgaaaaactg accatgcgtg aagcgatcaa gaaatatcgc ccggaaaccg
    46561 acatggcgga tctggataac ttcgactctg cgaaagcgat tgctgaatct atcggtatcc
    46621 acgttgagaa gagctggggt ctgggccgta tcgttaccga gatcttcgaa gaagtggcag
    46681 aagcacatct gattcagccg accttcatta ctgaatatcc ggcagaagtt tctccgctgg
    46741 cgcgtcgtaa cgacattaac ccggaaatca cagaccgctt tgagttcttc atcggtggtc
    46801 gtgaaatcgg taacggcttt agcgagctga atgacgcgga agatcaggcg caacgcttcc
    46861 tggatcaggt tgccgcgaaa gacgcaggtg acgacgaagc gatgttctac gatgaagatt
    46921 acgttaccgc actggaacat ggcttaccgc cgacagcagg tctggggatt ggcatcgacc
    46981 gtatggtaat gctgttcacc aacagccata ccatccgcga cgttattctg ttcccggcga
    47041 tgcgtccggt gaaataagca ttacgttatg ctcacaaccc cggcaaatgt cggggttttt
    47101 tatttaagct gggtaaatgc agataatcgt attctggctt cgcgatttgt cgcctgcatc
    47161 accatccacg gactgaacgc ccacggcgtg gcatcaatac cgcgtaatac atcagctaaa
    47221 tcacaccatt gataatccat cacttcatca tcgttgatct gtaacgcact ggtggtgcgt
    47281 gcggcaaata ccggacacac ttcattttcc acaatgcctc tcggatcggt ggcgcggtag
    47341 cgaaagtcag gatagataga ttcaggaggc gtaatttcca cgccaagctc ataacggcaa
    47401 cggcggatca ccgcgtcttc gctgctttct cccagttgtg ggtgcccaca aaccgagtta
    47461 gtccacacgc caggccacgc ttttttgctc agtgcgcggc gggtaactaa taattgcccc
    47521 ttggcattaa acagccaact ggagaacgcg agatgtaaga gggtgtctgc cgtgtgtgcg
    47581 gcatactttt ccagcgtacc cgtgggaact ccctgtgcat tcaataaaat gacgtgttcc
    47641 gtttgcataa tttctcacat gtaattctga tcactcatca ttatagataa ttgaagagtg
    47701 aaatgattaa ccaagagagc gtttatcgac aaccgcccgt tttgagcgga tgccgacagg
    47761 taaaaaccat ttaaaaaagt agcatcatat atttgaagcg actttcaatg gccattgcaa
    47821 cacagtcaga aatgacagga atagagagag taaaaagcaa caacagactg aatatagcgc
    47881 gcattaaaaa attcatatta ttctccatgc tcatttttct tcagcttgcg catcagaagc
    47941 cacatacgta ctgtcctgac ttctaaatct ttatcagaga cggattcctt caccaggtca
    48001 gcatgatgct gatagccatt aaagaagacg ttcagcacaa cggcgctaag ggttgcgagc
    48061 ataatgccgc tatgtagcag cggttgcagt acggccggta acttagaaaa gaaatcgtga
    48121 gagagcgtcg gagtcatgcc aacgccgaga ctgatcgcca caatatagag gttgtaacgg
    48181 ttggtggtgt agttacagcg cgacagaatt cgaatccctg tcgccagtac catgccgaac
    48241 atcactagac cagcgccgcc cagcacaaat tgcggaatgg aggctaccag caccgccatt
    48301 tttggcacca tgccaaacag gattaatata attcccgaag aaatacacac ccagcggcta
    48361 tgaacgcgca tcacgctaac caggccaacg ttttgagaaa atgacgtgtg ggggaagctg
    48421 ttaaacgtgc cgcctatcat tgtccctacg ccatcgacac gcagcccgca aataatatcg
    48481 tgtgaagaga gtttacgacc gactatttca cccagtgcca ggaacatccc catcgactcg
    48541 ataaacacga tgattaacac ggcagtcatg gtcagaatgg aaacgggatc gaaaatcggc
    48601 ataccaaacg acatcggggt gacaatcgca aaccacgagg catcatgtag cccggataaa
    48661 ttgacttcat tcatcatcca cgaaagtaaa aagccaaata caatccccag taatacggcg
    48721 acgttggaca taaatccttt cgcatagcga gtaatgagca agataaaaat taagacggca
    48781 aaggagatac ctaaataaac gggattacca tattgcggat tccctttacc tccagcggcc
    48841 cagtcaatac ccacctgaat gatgctaagc ccaatagaag taataaccac accggtaacc
    48901 agtggcggga ataaaggcat caagcgacca ataagtggcg ctaataatgt ggtgataaaa
    48961 cccgcggcga tagtggcacc aaatatcccc agcaggccga tatccgggtt catcccaatg
    49021 gctatcattg gtgttacagc agcaaaggtc accgacataa tcaccggcag gcggatcccc
    49081 ataaagcggc cgataccgat acattgcaat aatgtgacga tcccgcagca aaagagatcc
    49141 gagctaatga gcatcgcaat agcatctttt gagaggccca gtcggtcacc aatcataaga
    49201 ggaacagcga ctgcacctgc gtacatgaca aggacgtgtt gcagaccgag tatgatcagc
    49261 tttcctggtg ataatatgcg gtcaacctca tcagttgggc ggtcttgccc agaagatgag
    49321 ggaagttggg aatctatggc gctcatcagt gtttctcctt tcgcccgcca gaataaaaag
    49381 caaccgttta cgttaatgaa actgtctaaa cacatgctcg tggagtgcca gtttttgcga
    49441 ggaaggggag ttagagacag gaattgcgga ggcaatcaca taatcgtgct taaaccacga
    49501 aaatgacaag agaaactgac aatggaatta tctgtaattt tctcatattg atatttcatt
    49561 aatcattctg gcatgtcgca ctctcgcatt taatcgtttt tatctggata gcgctctttt
    49621 gatcggcaat gttgaaataa tgaaagcggc atagattgag gggcaataat ttgaactcag
    49681 tcgcaaaatt ataattaaaa cggtcatgct acctaatata tgcccgttta ttgcatcgcg
    49741 aaatttgaga agacataact cattaaatga aagggatgca aatgaatacg tttatcgctg
    49801 ctgaagctgc ggaatgtata ggctgtca
//
LOCUS       DAJTSA010000020        49537 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000020 DAJTSA010000000
VERSION     DAJTSA010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.020"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1150)
                     /gene="setB"
                     /locus_tag="OO369_001233"
     CDS             complement(<1..1150)
                     /gene="setB"
                     /locus_tag="OO369_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR5981578.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIAT"
     gene            1518..2648
                     /gene="fruB"
                     /locus_tag="OO369_001234"
     CDS             1518..2648
                     /gene="fruB"
                     /locus_tag="OO369_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR5981579.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2648..3586
                     /gene="fruK"
                     /locus_tag="OO369_001235"
     CDS             2648..3586
                     /gene="fruK"
                     /locus_tag="OO369_001235"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR5981580.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3603..5294
                     /gene="fruA"
                     /locus_tag="OO369_001236"
     CDS             3603..5294
                     /gene="fruA"
                     /locus_tag="OO369_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR5981581.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5718..6659
                     /locus_tag="OO369_001237"
     CDS             5718..6659
                     /locus_tag="OO369_001237"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR5981582.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6647..7585
                     /gene="psuG"
                     /locus_tag="OO369_001238"
     CDS             6647..7585
                     /gene="psuG"
                     /locus_tag="OO369_001238"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR5981583.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7679..8929
                     /locus_tag="OO369_001239"
     CDS             7679..8929
                     /locus_tag="OO369_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5981584.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9000..9694)
                     /gene="yeiL"
                     /locus_tag="OO369_001240"
                     /pseudo
     CDS             complement(9000..9694)
                     /gene="yeiL"
                     /locus_tag="OO369_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9825..10766
                     /gene="rihB"
                     /locus_tag="OO369_001241"
     CDS             9825..10766
                     /gene="rihB"
                     /locus_tag="OO369_001241"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR5981585.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10866..12116
                     /locus_tag="OO369_001242"
     CDS             10866..12116
                     /locus_tag="OO369_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5981586.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12172..13260)
                     /locus_tag="OO369_001243"
     CDS             complement(12172..13260)
                     /locus_tag="OO369_001243"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5981587.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="OO369_001244"
     CDS             complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="OO369_001244"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR5981588.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14194..15243)
                     /locus_tag="OO369_001245"
     CDS             complement(14194..15243)
                     /locus_tag="OO369_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR5981589.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15342..16223
                     /locus_tag="OO369_001246"
     CDS             15342..16223
                     /locus_tag="OO369_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5981590.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16429..17898
                     /gene="lysP"
                     /locus_tag="OO369_001247"
     CDS             16429..17898
                     /gene="lysP"
                     /locus_tag="OO369_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR5981591.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18190..20181
                     /gene="cirA"
                     /locus_tag="OO369_001248"
                     /pseudo
     CDS             18190..20181
                     /gene="cirA"
                     /locus_tag="OO369_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20213..21049)
                     /gene="yeiG"
                     /locus_tag="OO369_001249"
                     /pseudo
     CDS             complement(20213..21049)
                     /gene="yeiG"
                     /locus_tag="OO369_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21307..21975
                     /gene="folE"
                     /locus_tag="OO369_001250"
     CDS             21307..21975
                     /gene="folE"
                     /locus_tag="OO369_001250"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR5981592.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21992..23068
                     /locus_tag="OO369_001251"
     CDS             21992..23068
                     /locus_tag="OO369_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR5981593.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23210..24250
                     /gene="galS"
                     /locus_tag="OO369_001252"
     CDS             23210..24250
                     /gene="galS"
                     /locus_tag="OO369_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR5981594.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24530..25528
                     /gene="mglB"
                     /locus_tag="OO369_001253"
     CDS             24530..25528
                     /gene="mglB"
                     /locus_tag="OO369_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR5981595.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25589..27109
                     /gene="mglA"
                     /locus_tag="OO369_001254"
     CDS             25589..27109
                     /gene="mglA"
                     /locus_tag="OO369_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR5981596.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27125..28135
                     /gene="mglC"
                     /locus_tag="OO369_001255"
     CDS             27125..28135
                     /gene="mglC"
                     /locus_tag="OO369_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR5981597.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28171..29384)
                     /gene="preA"
                     /locus_tag="OO369_001256"
                     /pseudo
     CDS             complement(28171..29384)
                     /gene="preA"
                     /locus_tag="OO369_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29378..30616)
                     /gene="preT"
                     /locus_tag="OO369_001257"
     CDS             complement(29378..30616)
                     /gene="preT"
                     /locus_tag="OO369_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR5981598.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30810..31049)
                     /locus_tag="OO369_001258"
     CDS             complement(30810..31049)
                     /locus_tag="OO369_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR5981599.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31052..31771)
                     /gene="sanA"
                     /locus_tag="OO369_001259"
     CDS             complement(31052..31771)
                     /gene="sanA"
                     /locus_tag="OO369_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR5981600.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31921..32805)
                     /gene="cdd"
                     /locus_tag="OO369_001260"
     CDS             complement(31921..32805)
                     /gene="cdd"
                     /locus_tag="OO369_001260"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR5981601.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32935..33630)
                     /locus_tag="OO369_001261"
     CDS             complement(32935..33630)
                     /locus_tag="OO369_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR5981602.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33627..34025)
                     /locus_tag="OO369_001262"
     CDS             complement(33627..34025)
                     /locus_tag="OO369_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR5981603.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34264..35211
                     /gene="dusC"
                     /locus_tag="OO369_001263"
     CDS             34264..35211
                     /gene="dusC"
                     /locus_tag="OO369_001263"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR5981604.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35582..35665)
                     /gene="yohP"
                     /locus_tag="OO369_001264"
     CDS             complement(35582..35665)
                     /gene="yohP"
                     /locus_tag="OO369_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR5981605.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35889..37325
                     /gene="mdtQ"
                     /locus_tag="OO369_001265"
                     /pseudo
     CDS             35889..37325
                     /gene="mdtQ"
                     /locus_tag="OO369_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            37378..38145
                     /locus_tag="OO369_001266"
                     /pseudo
     CDS             37378..38145
                     /locus_tag="OO369_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38262..38840)
                     /locus_tag="OO369_001267"
     CDS             complement(38262..38840)
                     /locus_tag="OO369_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5981606.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39010..39597
                     /locus_tag="OO369_001268"
     CDS             39010..39597
                     /locus_tag="OO369_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR5981607.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39771..40703
                     /gene="pbpG"
                     /locus_tag="OO369_001269"
     CDS             39771..40703
                     /gene="pbpG"
                     /locus_tag="OO369_001269"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR5981608.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40741..42456)
                     /gene="dld"
                     /locus_tag="OO369_001270"
     CDS             complement(40741..42456)
                     /gene="dld"
                     /locus_tag="OO369_001270"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5981609.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42652..44949
                     /gene="bglX"
                     /locus_tag="OO369_001271"
     CDS             42652..44949
                     /gene="bglX"
                     /locus_tag="OO369_001271"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR5981610.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45201..46118
                     /gene="osmF"
                     /locus_tag="OO369_001272"
     CDS             45201..46118
                     /gene="osmF"
                     /locus_tag="OO369_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR5981611.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46125..47282
                     /gene="yehY"
                     /locus_tag="OO369_001273"
     CDS             46125..47282
                     /gene="yehY"
                     /locus_tag="OO369_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR5981612.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVPGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47275..48201
                     /gene="yehX"
                     /locus_tag="OO369_001274"
     CDS             47275..48201
                     /gene="yehX"
                     /locus_tag="OO369_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR5981613.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48206..48937
                     /gene="yehW"
                     /locus_tag="OO369_001275"
     CDS             48206..48937
                     /gene="yehW"
                     /locus_tag="OO369_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR5981614.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48918..49025)
                     /locus_tag="OO369_001276"
     CDS             complement(48918..49025)
                     /locus_tag="OO369_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR5981615.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49085..49537)
                     /locus_tag="OO369_001277"
                     /pseudo
     CDS             complement(49085..49537)
                     /locus_tag="OO369_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        11957 a        12206 c        13313 g        12061 t
ORIGIN      
        1 gagtggcgat aatcatcacc atcgcaaacc agaacacagc gtgataattc cagatctcgg
       61 cgacgatgcc cgccactgat cctgcgatga tccagcccac gcgcgacgtg ttggtataga
      121 gcgtggtggc tgaacctgcc tgaccgggca tcaaatcctg aaaatagagc atcccgatgc
      181 cgcccagaat gccaataaaa atagcattta gcagctgcaa gcccaacaga atggcaggtg
      241 aatgcgccat cagcattcct gcgtaaaaac agacgccacc cacggcagca acgcgcatta
      301 agaaacgctt acccagacgt ttggcgaaat atccggcaat caacatcgtc gggatttcca
      361 gcccggcggc ggtccccatc atcacaccgg ccagtttctc gggaagatgc agttcgttga
      421 taataaatag cggcatgttg atgatgtaca ggctgttcga gccccacatc aatgtacaaa
      481 tgacaaacag cagtagcgta tcgcgacggt tacggcgcgg cgcttcaacc gtgccggtcg
      541 ccagcggaag ctctttttgc atcgacggta aaaacagcca caccatcacg ccgcaaacaa
      601 taaacgctac cgctgcgctc agatacatta ccgtaaagct gaaacccatc gctaacgcat
      661 aagccagcgg tgggccaatg acccatgcca gtgaaacctg agcgcgtaaa aaagagctga
      721 acatcaccgc ctcacgtccg gttttgtcgg catgttcacg ggcaagggca aacatttgcg
      781 ggttagcggt cgagccaaag ctgctaagaa agacgccaac gaatagcaaa acaaagtagt
      841 tgcgattcca agcaaaaagg gtgcaggcca gcacgcctaa caggcagcaa aagacaatca
      901 gcgatttgcg atcgccgcgc ttatcagagc gcccagcgag aaactgactt accagaatcc
      961 caatgacagc gctgccggta aagaagaatc ccaccatcgc cggacgggca tgtacttcat
     1021 cggtaaggaa aatactgagt gtcggggttt gcagagcacc cgcaataccg gtgagaaagg
     1081 caacgattaa aaacgccgtc gaggtcaggt caaacgattt cgcgctggag actgcggggg
     1141 agttatgcat gctggtcgat cactggaaga ggaagacgcg gagtttacgc tgcttgtcag
     1201 aaaataaaca gacagcgaaa tcaaaagcgc cacgaataat caaaatgcca tttcactgtt
     1261 gctgattggc tgctgacgct gctgaaatga aattgctgat aacggatttt cccatcagca
     1321 attaggaaaa atgacaaaaa attgtgcagc acatcaaact tttgctcata actttacggc
     1381 tttccttgcg tgctgaaaca gaagtattat gctttcttga aacgtttcag cgcgatcttg
     1441 tctttaaccc taagctaggt tggcgctttt tttctcatag aggctgaatc gtttcaattc
     1501 agcaagagag gagaactatg ttccagttat ccgtacagga catccatccg ggcgaaaagg
     1561 ccggagacaa agaagaggcg attcgccagg tcgctgcggc gctggtgcag gccggtaatg
     1621 tagcagaagg ctacgtcaat ggcatgctgg cgcgcgaaca gcaaacctca acgttcctcg
     1681 gcaatggtat tgctattcca cacggcacta ccgacacccg cgatcaggtg ctgaaaaccg
     1741 gcgttcaggt atttcagttc ccggaaggcg tcacctgggg tgacggtcag gtagcgtacg
     1801 tggcaatcgg tattgctgcc agctcggata aacatctggg cctgctacgc cagctgaccc
     1861 acgtactgag cgatgattcc gttgctgaac aactgaagtc agcaacaaca gcagaagaac
     1921 ttcgcgcatt actgatgggc gaaaagcaga gtgagcagct gaagctcgac aacgaaatgc
     1981 tgacgctgga tatcgtcgcc agcgatctgc tgactcttca ggcgctgaac gctgcgcgtc
     2041 tgaaagaggc gagggcagtt gacgccactt tcgtcaccaa agccatcaat gaacaaccgc
     2101 tgaatctcgg acagggtatc tggctgagcg atagcgccga aggcaatctg cgtagtgcga
     2161 ttgcggtaag ccgtgcggca aatgcttttg atgtggacgg cgaaacggca gccatgctgg
     2221 tgagtgtggc gatgaatgac gatcagccca tcgcggttct taagcgtctc gctgatttgt
     2281 tgctcgacaa taaagctgac cgcttgctga aagcggatgc ggcaacgttg ctggcgctgc
     2341 tgaccagcga tgatgcgccg accgacgacg tgttaagcgc ggagtttgtg gtgcgcaatg
     2401 aacacggcct gcatgctcgt ccaggtacca tgctggtcaa taccattaaa caatttaaca
     2461 gtgatattac cgtgacaaac cttgatggca ccggcaaacc ggcaaacgga cgtagtctga
     2521 tgaaagttgt ggcacttggc gttaagaaag gtcatcgcct acgctttacc gcccagggtg
     2581 cagatgctga acaggcgctg aaagccattg gcgacgctat cgctgctggt cttggggagg
     2641 gcgcataatg agcagacgtg ttgctacgat cacccttaat ccggcttatg accttgttgg
     2701 tttctgcccg gaaattgaac gcggcgaagt gaacctggtg aaaaccaccg gtctgcatgc
     2761 ggcgggtaaa ggcatcaacg tggccaaagt attaaaagac ctgggaattg atgtcaccgt
     2821 tggcggcttc ctgggtaaag acaatcagga tggttttcag caactgttca gcgagctggg
     2881 cattgccaac cgtttccagg ttgtacaggg gcgcacccga attaacgtta agctgacgga
     2941 aaaagacggc gaagtgaccg acttcaactt ctcgggtttc gaagtcaccc ccgccgactg
     3001 ggaacgcttt gtgactgatt ctctgagctg gctcggtcag ttcgatatgg tctgtgtcag
     3061 cggaagctta ccgtcaggcg tcagcccgga agcgttcacc gactggatga ctcgcctgcg
     3121 tagtcagtgt ccttgcatta tctttgatag tagccgtgaa gcgttagtag caggtttgaa
     3181 agcggcaccg tggctggtga aacctaaccg ccgcgagttg gaaatctggg caggccgtaa
     3241 actgcctgaa atgaaagatg tgattgaagc tgcacatgcg ctacgtgaac aaggtattgc
     3301 gcatgttgtt atttcactgg gtgccgaagg cgcgctttgg gttaatgcct ccggcgaatg
     3361 gatcgctaaa ccaccgtcag tcgatgtcgt aagcaccgtt ggcgcagggg attctatggt
     3421 tggtggcctg atttatggct tgctgatgcg tgaatccagt gaacacacac tgcgtctggc
     3481 gacagctgtt gcagccctgg cggtaagtca aagcaatgtg ggtattaccg atcgtccgca
     3541 gttggccgca atgatggcgc gcgtcgactt acaacctttt aactgacagc aggagaggca
     3601 taatgaaaac gctgctgatt attgacgcta atctcggtca ggcacgcgcc tatatggcga
     3661 agaccctgct gggcgcggcg gcgcgaaaag caaaactgga aatcatcgac aatccgaacg
     3721 acgcagaaat ggcgattgtt ctcggtgatt ccatcccgaa tgacagcgcg ctgaacggta
     3781 aaaatgtctg gctgggtgat atttcccggg cagttgcgca ccctgagctg ttcctgagtg
     3841 aagccaaagg ccatgcgaaa ccttacactg cgccggtcac tgcgacagca ccggttgccg
     3901 ccagcggtcc gaaacgcgta gttgcggtga ctgcttgccc gactggcgta gcacacacct
     3961 ttatggcggc tgaagccatt gaaaccgaag cgaaaaaacg tggctggtgg gtgaaagttg
     4021 aaacccgtgg ttctgttggc gcgggtaatg caatcactcc cgaggaagta gccgcagcgg
     4081 atctggtgat tgtggcggca gatatcgaag tggatctggc gaaatttgct ggtaaaccga
     4141 tgtatcgtac ctctaccggt ctggcgctga agaaaacctc gcaggaactg gataaagcgg
     4201 ttgctgaagc aacgccgtat gaaccggcgg gcaaagctca aacggcgacc actgaaggta
     4261 agaaagagag tgcaggcgct tatcgtcact tgctaacggg cgtctcttac atgctgccga
     4321 tggtcgttgc tggtggtctg tgtatcgcgc tttcttttgc ttttggtatc gaagcgttta
     4381 aagagccggg tacgttggca gcggcgctga tgcagattgg tggtggttca gcctttgcgc
     4441 tgatggtgcc ggtactggca ggttatattg ccttctccat tgccgatcgt ccgggcctca
     4501 cgccgggtct gattggcggt atgctggcgg tcagcaccgg ttctggcttc attggcggta
     4561 ttattgcggg cttcctggct ggttacattg cgaagttaat cagtacgcaa ctgaaactgc
     4621 cgcagagtat ggaggcgctg aaaccgatcc tgatcattcc gctaatttcc agtctggtgg
     4681 tcggtctggc gatgatctac ctgatcggta aaccagttgc tggcattctc gaagggctga
     4741 ctcactggct gcagaccatg gggactgcga atgcggttct gctgggggcg atcctcggtg
     4801 gcatgatgtg tactgacatg ggcggtccgg taaacaaagc agcgtacgca ttcggtgtgg
     4861 gtctgctgag tactcaaacc tatggcccga tggcggcgat tatggcggca ggtatggtgc
     4921 caccgctggc aatgggcctg gcaacaatgg tggcgcgtcg caaattcgac aaagcgcagc
     4981 aggaaggtgg caaagccgct ctggtattgg gactgtgctt catttcggaa ggtgcaattc
     5041 cgtttgctgc tcgtgatccg atgcgtgtgc tgccgtgctg tatcgtgggt ggggcgctga
     5101 ctggcgcaat ctcaatggcg attggtgcga aactgatggc accgcacggt ggtctgtttg
     5161 ttctgctgat ccctggcgct attacgccgg tattgggtta cctggtagca attattgccg
     5221 gtacgctggt ggcgggtttg gcctatgcct tcctgaaacg tccggaagtg gacgcagtag
     5281 cgaaagcagc gtaataaaag gtgtgttaca gggcagaaat ttcctgccct gtaacatttg
     5341 atgagcgcaa agagaatgac cagcttgtga atgtaatttt atgaatttaa atggttttat
     5401 ctttcagtag aaacttcaaa cgttcatcaa acactaaggt tatttcctca agggcagatt
     5461 attttctgcc cttttttagt gttcctgttc tgtgcatccc gtcacaaatt cctcgctttt
     5521 gtaattcagg catttaatcg cattttgtga gcctgctcaa atctgcccat tttgcctctc
     5581 tggcggtaat ttactttacg taaaacatgc atactatgag cacatgttta aatatgaaca
     5641 catgtttaaa ccgtctttgc agtatctata ttcttgtgga ttgacagggt tggtaatttt
     5701 ttaccagagg acgtgctatg cgcgaaaagg attatgtcgt aattataggt tcggcgaata
     5761 ttgatgtcgc cggatattca catgaatcat taaattatgc ggattcaaat ccaggtaaaa
     5821 taaaatttac gcctggtgga gtagggcgca atattgcaca aaacctggcg ttgctgggta
     5881 acaaagcctg gctactgagc gccgtaggca gtgattttta tggtcaatcg ctgctaacgc
     5941 aaaccaatca atctggtgtt tatgtcgata aatgcctgat tgtgccggga gaaaatacgt
     6001 cgagttattt atcattactc gataataccg gtgaaatgct ggttgctata aatgacatga
     6061 atattagcaa cgctattaca gctgaatatc tcgcacagca ccgtgaattt attcagaggg
     6121 caaaggtcat tgtcgcggac tgtaatatca gtgaagaggc actggcatgg attctggata
     6181 atgccgccaa cgtacccgta tttgtcgatc cggtttcagc gtggaaatgt gtcaaagtgc
     6241 gcgaccgtct aaatcagaac cacactctga agccaaaccg ccttgaagcg gaaaccctga
     6301 gtgggattgc gctgtcaggg cgtgacgatg tggcaaaagt tgctgcctgg ttccatcaac
     6361 atggcctgaa ccgactggta ttgagcatgg gcggcgacgg cgtttattac agcgatatca
     6421 gcggtgaaaa tggctggtct gcgccgatca aaaccaatgt tattaatgtt accggagcgg
     6481 gcgatgccat gatggcggga cttgcttcgt gttgggtaga cggaatgccg tttgccgaat
     6541 ctgttcgttt cgcacaggga tgttcgtcaa tggcgctctc ctgtgaatac accaataacc
     6601 ccgatttatc gattgccaac gttatatcgt tagtggagaa cgcagaatgt ctgaattaaa
     6661 aatttcccct gaattattac aaatttctcc ggaagtgcag gacgctttaa aaaacaaaaa
     6721 accggttgtg gcgctggaat cgaccattat ttctcacggg atgccgttcc cacaaaatgc
     6781 ccagaccgca attgaagtag aagaaactat tcgtaaacag ggcgcagtac ctgccactat
     6841 cgccattatt ggcggcgtga tgaaagtggg tttaagcaaa gaaaaaattg aattactggg
     6901 tcgtgaaggg cataacgtga ccaaagttag tcgtcgcgat ttaccttttg ttgttgccgc
     6961 cggaaaaaat ggcgcaacca ctgtggcttc aacgatgatt attgcggcgc ttgccggaat
     7021 taaagtattt gccaccgggg gaattggtgg tgtgcatcgc ggggcggaac ataccttcga
     7081 tatttctgcc gatttgcaag aactggcaaa tactaatgtc accgttgttt gtgccggggc
     7141 gaaatctatt ctcgatttag gattaaccac tgagtattta gaaaccttcg gtgtgccgtt
     7201 aattggctat cagactaaag cgctgcctgc gtttttctgc cgtaccagct cgtttgacgt
     7261 cagcattcgt ctcgacagcg ccagtgaaat tgcccgtgca atggcggtga aatggcaaag
     7321 cgggctgaac ggtggcctcg tggtagcgaa cccgatcccg gaacagtttg cgatgccgga
     7381 ggaatctatc aatgcagcca tagatcaagc cgtcgccgaa gccgaagagc agggcgttat
     7441 tggtaaagaa agtacaccgt tcctgctggc gcgcgttgct gagctgacca gcggtgacag
     7501 cctgaaatcc aatatccagc tggtgttcaa caacgccatt ctggcgagtg aaattgccaa
     7561 agaataccag cgtctcgcgg gttaatttct gacgggcaga aagccgtcgc ccgtactcat
     7621 caagcatacc aaaacctggc gctgacgccg ggtttactgg catgaaggga aaatcattat
     7681 ggatataatg agaagtgttg tggggatggt ggtgttactg gcaatagcat ttctgttgtc
     7741 agtgaataaa aagagcatca gtttgcgcac ggttggagcc gcactgctgc tgcaaatcgc
     7801 tattggtggc atcatgctct acttcccacc gggaaaatgg gcagtagaac aggcggcatt
     7861 aggcgttcat aaagtgatgt cttacagtga tgccggtagc gccttcattt ttggttcgct
     7921 ggttgggccg aaaatggatg tcctgtttga cggtgcgggt tttatcttcg cctttcgcgt
     7981 acttccggcg attattttcg ttactgcgct catcagtctg ctgtactaca ttggcgtgat
     8041 ggggctgctg attcgcatcc ttggcagcat tttccagaaa gccctcaaca tcagcaaaat
     8101 cgaatctttt gttgcggtta ccactatttt cctcgggcaa aatgagatcc cggcgatcgt
     8161 taaaccgttt atcgatcgcg tgaatcgcaa cgagttgttt accgcaattt gtagcgggat
     8221 ggcgtccatt gctggttcgg tgatgattgg ttatgccgga atgggcgtac caattgacta
     8281 cctgttagcg gcatcgctga tggcgatccc cggcggtatt ttgtttgcac gtattcttag
     8341 cccggcaacc gagccttcgc aggtcacatt tgaaaatctg tcgttcagcg aaacgccgcc
     8401 aaaaagcttt atcgaagcgg cggcgagcgg tgcgatgacc gggctgaaaa tcgccgctgg
     8461 tgtagcgacg gtggtaatgg cgtttgtcgc aattattgcg ctgatcaacg gcattatcgg
     8521 cggaattggc ggctggtttg gtttcgccaa tgcctctctg gaaagtattt ttggctatgt
     8581 gctggcaccg ctggcgtgga tcatgggtgt ggactggagt gatgccaacc ttgctggtag
     8641 cctgattggg cagaaactgg cgattaacga attcgtcgct tacctgagtt tctccccata
     8701 cctgcaaacg ggcggcacgc tggaagtgaa aaccgtggcg attatatcct ttgcgctttg
     8761 tggttttgct aactttggtt ctatcggtgt tgtcgttggc gcattttcgg ctatttcgcc
     8821 aaaacgtgct ccagaaatcg cccagcttgg tttacgggcg ctggcagcag caacgctttc
     8881 caacctgatg agtgcgacta ttgccgggtt ctttattggt ctggcgtaat gctcgatttg
     8941 tagaccggat aaaacgtatt aagcgttgca tccggcaatc gcgctattgc aaataatttt
     9001 tactgcatca tcccggagaa tttattctcc gggtccatct ccagcgccag tcctgacaac
     9061 tgctttctgt ttttaattag atatcctttc ttttaattaa taaaccgtcg tgaatgaatt
     9121 gcgcgagaac atataaaaga tgtcgataag aaacgcctaa atactctgcc gcttgcgtat
     9181 gcttttcgtg ataaagatca ccttcttgtg agagtaatat aaatgctgcc aggcgattca
     9241 ctaacggaaa tgattgattc tgagttaaag aaacaatatt acgataattt ttatgactta
     9301 aggtgacgca aagttttcgt aaaaataggg tgtcgtttaa taacagcgga cggtaatgtt
     9361 tcatagggag cgcaaggcac caacactctt caatagcctg caccgcacgc ggttcatgat
     9421 ctttgtcgat taactcaatc tcgccaataa aacagggagc ggcaaagaaa tcgatcagcg
     9481 acacgcgacc attggcaagc gtggcgtaaa gcctggcgcg gcctcgcgtc agataaaaca
     9541 gccatgacgg ctgttgccct tcctgcacaa tgtagtcacg cgctaaaaaa tgaaacagcc
     9601 gcgtatcggc tgaaacatcc gttgaaaagc aatccttaaa cgcagattca ctcatcaatt
     9661 gctgcttaag gtcattattg tggatttctt tcacgatgtt tatccgctat gagatttctc
     9721 atatttagca tacgtacgct gttgttaaga ttcaatcatt ggcccggtcg aattttcttt
     9781 gctgtagaaa aaaataacgc aactggaaac agaggaaata aacaatggaa aagagaaaaa
     9841 ttattctgga ttgtgagccg ggtcatgatg atgctattgc aatgatgatg gcagcgaaac
     9901 atccggcaat agatttatta ggcatcacta ttgtagcggg taatcagacg cttgataaaa
     9961 cattaattaa tggcctgaat gtttgccaga aactggagat taatgttccg gtttatgcgg
    10021 ggatgccgca acccattatg cgtaaacaaa tcgttgccga taatattcac ggtgaaaccg
    10081 gactggatgg cccggtattc gagccgctga cccgccaggc agaaagtacc catgcggtga
    10141 aatatatcat cgataccctg atggcaagcg atggcgatat cactctggtg ccggttggtc
    10201 cgctttcaaa tatcgcggtg gcaatgcgta tgcaacccgc aatcctgccc aaaatccgtg
    10261 aaattgtgct aatgggcggc gcttacggta caggcaactt caccccatct gccgagttca
    10321 atatctttgc cgacccggaa gccgcccgcg tggtgttcac ctccggcgtt ccattagtga
    10381 tgatgggcct cgatcttacc aaccagaccg tttgcacccc ggatgtaatt gctcggatgg
    10441 aaagggtagg cggccctgcc ggagagctgt tcagcgacat catgaacttc actctcaaaa
    10501 cgcagttcga atactacggc ctggctggcg gcccggtgca tgacgccacc tgcattggtt
    10561 atctgatcaa ccctgatggc attaaaaccc aggatatgta cgtcgaagtg gacgtcaaca
    10621 gcggcccttg ctacgggcgt acggtctgcg acgaactggg cgttcttggc aagcccgcta
    10681 ataccaaagt cggcatcact attgataccg actggttctg gggcttagtc gaagagtgcg
    10741 tgcgcggcta catcaaaacc cattaagtgt tgataacaag ccgggacagt atcgcaagat
    10801 gcaggcccgg ctatttctga tgggcagaaa gccgtcgccc aaaatgacat gaagagataa
    10861 tcactatgga tgtcatgaga agtgttctgg gaatggtggt actgctggca attgcgtttt
    10921 tactgtcagt aaacaagaag aagatcagcc tgcgtaccgt tggcgcggcg ttagtgttac
    10981 aggtcgtgat tggcgggatt atgctttggt taccgccagg gcgttgggtc gcagaaaaag
    11041 tcgcttttgg cgtccacaaa gtgatggcgt acagcgacgc aggtagcgca tttatcttcg
    11101 gttctctggt cggaccgaaa atggatacct tatttgatgg cgcaggattt atctttggtt
    11161 tcagggtatt accggcaatt atcttcgtca ctgcactggt gagtattctc tactacatcg
    11221 gtgtgatggg gatcttaatt cgcattctcg gcggtatctt ccagaaagct ttaaatatca
    11281 gcaagatcga gtcattcgtc gcggtcacca ccattttcct cgggcaaaac gaaattccgg
    11341 cgatcgtcaa accctttatc gatcgtctga atcgcaatga attatttaca gcgatttgta
    11401 gtggcatggc ctcgattgct ggttcgacaa tgattggtta cgccgcactg ggcgtacctg
    11461 tggaatattt gctggcggca tcgttaatgg cgatccctgg cgggatcttg tttgcccgcc
    11521 tgttaagccc ggctacggaa tcttcgcagg tttcttttaa taacctctct ttcaccgaaa
    11581 caccgccaaa aagcattatt gaagccgatg cgacaggggc aatgaccggg ctgaaaatcg
    11641 ccgccggtgt agcgacagtt gttatggcat ttgtcgccat cattgcgtta attaacggta
    11701 ttatcggcgg cgttggcggc tggtttggtt ttgaacatgc ctcgctggag tccattttag
    11761 gttacctgct ggccccactt gcgtgggtaa tgggtgttga ctggagtgat gcaaatcttg
    11821 ccgggagttt gattggacag aaactggcaa taaatgaatt tgtcgcttat ctcaatttct
    11881 caccctatct gcaaacggct ggcactctgg atgctaaaac cgtggcgatt atttccttcg
    11941 cgttgtgcgg tttcgctaac tttggttcta tcggggtggt ggtgggggcg ttttctgcgg
    12001 ttgcgccaca ccgtgcgccg gaaatcgccc agcttggttt acgcgcgctg gcggcggcga
    12061 cactttctaa tctgatgagt gccaccattg ccgggttctt tattggttta gcgtagtgtg
    12121 caatttatga gccggattag atgcggtcaa gcgtcgaatc cggcaacatt gttacaccat
    12181 tggcactaat gacagcgcgt tatcggcaga cagggtaggg ttgtttaagc tgccgctggc
    12241 gcgtgaaatt gccgcgcagg ccatcgcaaa acgggcgctg tcacggaaat tgtttccctc
    12301 cagaaagcta tataccagac ccgccataaa accatcgtca gcaccaaaac tgtctaccgt
    12361 cgtatgcgct ggcacagtca gcaaaaattg ttcaccatcc ttttcgctgc aataaactga
    12421 ctcatcgggc aaataaacaa acagttgctg aacaccttgc tgatgcaatg cattcactgc
    12481 ggcattacgg tcagcatcgc tggtgatcgc ctgtccccat aaaatttcca gctccggtaa
    12541 agtgggtttc agggtgtgaa tatgcgccag ccagtgtttg attttgcccg ctttgaattc
    12601 tgaaacggta tcgacaaaca ccgggatctc atcagcgagg gtaaagaccc attccagcgc
    12661 ctcggctgtc aggttgcaat cagccagtac cacgcccgca tgacgaagta aatcgcgcga
    12721 cccgttcagt agttgcggtg tcaactgttc cagcagatgg gtatcgttaa ttgccagcac
    12781 ggtttgatcg tctcgattgg caattgccag atacgtcgat gtgctttgac catgcaaacg
    12841 aacgcagccg gagacattca cgccggcacg gcgcgtttct tccaggagca tttcgccata
    12901 aaagtcatcg ccaatcactg aaagcaaatg gacgtcacgg cctaacagcg ccagattgtg
    12961 ggcgatattg cgtccaacgc cgcctgccga acaatgaatt gtaccgggat gagaagccgc
    13021 ttgcgggtaa tggatatccg ccatcccgcg aatatccata ttgattgtcc ccaccactac
    13081 gcagtattcc tgctcggtga gaatgtaacc tttgtcttta atccggcctt tgcgcattaa
    13141 atccataata tgcgccgcaa cgcgcgaacg actgatttgc agcatgtccg caatttcgtt
    13201 ctgctgaatc agcgggttac gccgtaaaat tgcaaggatc tccttttccc ggttattcat
    13261 cttcaggcta ccgctttttc agtttgttgc gctttcagcc aggcgatctc ttctgcccag
    13321 atatccgggt tgatggtttc aaggatcagc ggaatgccgt cgaaacggtc gttctgcata
    13381 atccagcgga acgcatcatg accgatattg ccttcaccga ggctatgatg gcggtcaacg
    13441 cggctgccaa aggtactttt cgcatcgtta aggtgcatcc cgcgcaaata cttaaagccg
    13501 acaatacggg caaaatcagc gaacgttttt tcgcattcgg ctggagtacg caaatcatac
    13561 ccggcagcga aagcatggca ggtatcaatg cagacgccga cgcgggattt atcttccacg
    13621 ccgtcgataa tcgccgcgag atgttcgaat ttaaacccta agttactgcc ctgaccggcg
    13681 gtgttttcaa tcaccgctgt tacaccttga gttttatcca gcgcaatgtt gatggattcg
    13741 gcaatacgcg caaggcaatc ctcttctgaa atctgcatca gatggctgcc agggtggaag
    13801 ttgagcaaag aaagccccag ttgttcgcaa cgctgcattt catctataaa ggcatcgcgc
    13861 gatttttcca gagcttcagt gaccggatgt ccgaggttga tcagataact gtcgtgggga
    13921 agaatttgcg ccgatgtgta gtgatatttt tcacaggcgg ctttgaattc atcgatggtt
    13981 tgcgtcgtga gcggtgcggc acgccactga cgttggtttt tggtgaacaa ggcaaacgcg
    14041 gttgcgtcga tttcggcggc gcgaattgcg gcatttgcca gaccgccagc agcactaacg
    14101 tgcgctccaa tgtatttcat gcgaggactc ctgttaaacc cgctggagga aaacggtaat
    14161 gatagcgggt taacaggagc aagaagtagt ggtttatgcg atgacgcttt gaatcacata
    14221 gttaatcgca ccaccaccaa caatcagcca ggcaaacagt accagtgcca tcagcagagg
    14281 tttcgcccca gcttttttca gcgcgctgac gtgagtggtc agacccagcg ccgccatcgc
    14341 cattgccagc aggaaggtat ccagcgttac cagcatgttc accacgctct gcggtaacag
    14401 gtggaacgag ttaaagatgg caactacgat gaacaagatg gcaaaccacg gaatagtgat
    14461 tttgcttttc tcgccgctgt tcgccccaga cagctgttta acacgcgccg ccagcaggat
    14521 gaggaacgga gccagcatca tcacgcgcag cattttggaa ataactgctg cgttttctgc
    14581 atccgggctg atggcatgac ctgccgccac cacctgcgcc acttcgtgca cagtagaacc
    14641 aatgtagata ccgaaagttt ccggactaaa ccattgagac atcagcggat atatcgccgg
    14701 gtagaggaaa atcgcgacgg tcccgaagat aacaacggtt gcaacagcca cggttacttt
    14761 actggcttcc gctttcacta ccggctcagt cgccagtacc gcggcagcac cacagatact
    14821 gctaccggca ccgatcaacc aactggtgtg cttatccaga ccaaacactt tctgccccag
    14881 gaagcaagcc agcaggaagg tactggacag cgtcagcacg tcaatgatga tcccactgat
    14941 accgacatcg gcaatttgcg agaacgtcag acggaagcca taaagaatga tacccagacg
    15001 taataaatat tgcttggcaa acagcacacc accgtcacag cttttccaga tgtgcggata
    15061 gatggtgttg cctaaaacca tccccaacaa gattgcgagg gtgagggcac taaacccggc
    15121 acccgcaacc gcgggaatgg aaccacccca cagggcgacc ccggtgataa ctgcactcag
    15181 ggctaacccc ggaataaaat gccacagtgt acgatgttgt ttctgtaagg tgatattcgt
    15241 cataaccctc tcctttaacc gggcataagg ttacgactaa ctggtttaaa aataaaattg
    15301 attatatatt tataattaat ctttataagt ggtaagcgac tatgcacatc accctccggc
    15361 agttggaagt ttttgcagaa gtattgaaaa gtggatcaac cacccaggcg tcggtgatgc
    15421 tggcgttgtc gcaatcagca gtgagcgcag ccttgactga cctggaaggg cagcttggcg
    15481 ttcaactgtt tgatcgcgtg gggaaaagac tggttgttaa tgaacacggg cggctgctct
    15541 atccgcgagc gttggcactg cttgaacagg cggttgaaat cgaacaactg tttcgcgaag
    15601 acaacggcgc gattcgtatc tatgccagta gtaccatcgg taactacatt ctgcctgcag
    15661 ttatcgcccg ttatcgccat gattatccgc agttgccgat tgaacttagc gttgggaaca
    15721 gccaggacgt gatgcaagcg gtgctggatt tccgcgttga tattggcttt attgaaggac
    15781 cgtgccacag cactgaaatc atttctgaac cgtggctgga agacgagctg gtggttttcg
    15841 ccgcgccgac ttcgccgttg gctcgtggtc cggtcacctt agaacagctg gccgctgcgc
    15901 cgtggatcct gcgtgaacgc ggttccggca cgcgggatat tgtcgattat ctgttgctgt
    15961 cacatttacc gaagtttgag atggcgatgg aattaggtaa ctccgaggca atcaaacatg
    16021 cggtgcgtca tgggttggga attagttgcc tgtcgcgacg tgtgattgaa gatcaattgc
    16081 aggcaggcac attaagtgaa gttgcggtcc ctctgccgcg cctgatgcgt acgttgtggc
    16141 gtatacatca tcggcaaaaa cacctttcca acgcgctacg gcgctttctg gactattgcg
    16201 atcccgcaaa tgtgccgcgt taagttgctg tacaagaaca tgctggtgct gtgtcgattt
    16261 cgtgacgcag cgccttcagc atgcattcgc cagaaaagag attggctgct ttacttataa
    16321 tccctgggcg atcatgaagg tgtcttataa ccgtgtattt ctgccggaag gattgccaat
    16381 cgtctgctac aatcgcgcca tcatttttaa gatggatagc atttttgtat gggttccgaa
    16441 actaaaacta cagaagcgcc gggcttacgc cgtgaattaa aggcgcgtca cctgacgatg
    16501 attgccattg gcggttccat cggtacaggt ctttttgttg cctctggcgc aacgatttct
    16561 caggcaggtc cgggcggggc attgctctcg tatatgctga ttggcctgat ggtttacttc
    16621 ctgatgacca gtctcggtga attggctgca tatatgccgg tttccggttc gtttgccact
    16681 tacggtcaga actatgttga agaaggcttt ggcttcgcgc tgggctggaa ctactggtac
    16741 aactgggcgg tgactattgc cgttgacctg gttgcagcac agctggtcat gagctggtgg
    16801 ttcccggata caccgggctg gatctggagt gcgttgttcc tcggcgttat cttcctgttg
    16861 aactacatct ctgttcgcgg ctttggtgaa gcggaatact ggttctcact gatcaaagtc
    16921 acgacagtta ttgtctttat catcgttggt gtgctgatga ttatcggtat cttcaaaggc
    16981 gcgcagcctg cgggctggag caactggaca atcggcgaag cgccgtttgc tggtggtttt
    17041 gcggcgatga tcggcgtagc tatgattgtc ggcttctctt tccagggaac cgagctgatc
    17101 ggtattgctg caggcgagtc cgaagatccg gcgaaaaaca ttccacgcgc ggtacgtcag
    17161 gtattctggc gaatcctgtt gttctatgtg ttcgcgatcc tgattatcag cctgattatt
    17221 ccgtataccg atccgagcct gctgcgtaac gatgttaaag acatcagcgt cagtccgttc
    17281 accctggtgt tccagcacgc gggtctgctc tctgcggcgg cggtgatgaa cgcagttatt
    17341 ctgacggcgg tgctgtcagc aggtaactcc ggtatgtatg cgtctactcg tatgctgtac
    17401 accctggcgt gtgacggtaa agcgccgcgc attttcgcta aattgtcgcg tggtggcgtg
    17461 ccgcgtaatg cgctgtatgc gacgacggtg attgccggtt tgtgcttcct gacctccatg
    17521 tttggcaacc agacggtata cctgtggctg ctgaacacct ccgggatgac gggttttatc
    17581 gcctggctgg ggattgccat tagccactat cgcttccgtc gcggttacgt attgcaggga
    17641 cacgacatta acgatctgcc gtaccgttca ggtttcttcc cactggggcc gatcttcgca
    17701 ttcattctgt gtctgattat cactttgggc cagaactacg aagcgttcct gaaagatacc
    17761 attgactggg gcggcgtagc ggcaacgtat attggtatcc cgctgttcct gattatttgg
    17821 ttcggctaca agctgattaa aggaactcac ttcgtacgct acagcgaaat gaagttcccg
    17881 cagaacgata agaaataagt ttcctccctt ccttgctaag ccctctcaac cgagagggct
    17941 ttttcaattc catttccctg acaaatcatg cggatataaa atttaacatt tggattgata
    18001 attgttatcg tttgcattat cgttacgacg caatcaaaaa aggctgacaa atcagaggct
    18061 gttccggctt tctgggatga tcacctgcat acaaataagt ccaccgcgat gctgccgtac
    18121 gcaaggggac gtaaagaaga tgtgagcgat acccatttta ttttcgtagt tacctcatgg
    18181 agatatggaa tgtttaggtt gaaccctttc gtacgggtcg ggctgtgttt gtccgctatt
    18241 tcttgtgcat ggcctgtgtt agcggtcgat gatgatggcg aaacgatggt tgtcactgca
    18301 tcttccgtgg aacaaaatct taaagatgca cccgccagta tcagcgtcat tacccaggaa
    18361 gacctgcagc gaaaaccggt acagaatctg aaggatgtcc tcaaagaagt gcctggcgta
    18421 caactgacga acgaagggga taaccgtaag ggcgttagta ttcgtggtct ggacagcagc
    18481 tacaccctga ttcttgtcga cggtaaacgc gttaactccc gcaatgccgt cttccgccac
    18541 aatgatttcg atctgaactg gatcccggtc gattccatcg aacgtattga agtggttcgt
    18601 ggcccgatgt cgtcgctata cggctccgat gcgctcggcg gtgtagtgaa tatcatcacc
    18661 aaaaaaatcg gtcagaaatg gtcgggtacc gttaccgtcg ataccaccat tcaggaacat
    18721 cgcgatcgcg gtgataccta taacggtcag ttctttacca gtggaccatt aattgatggt
    18781 gtgctgggaa tgaaagctta cggcagcctg gcaaaacgtg aaaaggatga cccgcaaaac
    18841 tcaacgacca ccgataccgg agaaacgccg cgtattgaag gattctccag ccgcgacggc
    18901 aatgtcgaat ttgcctagac accgaatcaa aatcacgatt ttactgccgg atacggtttc
    18961 gaccgtcagg atcgtgattc cgattcgctg gacaaaaacc gcctggaacg ccagaactac
    19021 tgcgtcagcc ataatgggcg ttgggattac ggcaccagcg aactgaaatc ctacggtgag
    19081 aaagtcgaga acaaaaaccc tggcaacagc agcccgataa cttccgaaag caatacggtc
    19141 gacggcaaat acacgttgcc gctgacggcg attaatcagt ttctcacggt tggcggtgaa
    19201 tggcgtcacg acaaacttag cgatgcggtg aacctgaccg ggggaaccag ctccaaaacg
    19261 tctgccagcc agtacgcgct gtttgtggaa gatgaatggc ggatcttcga gccgctggcg
    19321 ctgacgaccg gcgtgcgtat ggacgatcac gaaacctacg gtgaacactg gagtccgcgt
    19381 gcctacatgg tttataacgc caccgacacc gtaacggtga aagggggctg ggcgacggca
    19441 tttaaagcac cttctctgtt gcaacttagc cctgactgga cgagcaattc ctgccgtggc
    19501 gcatgtaaga ttgtgggtag cccggatctg aaaccagaaa ccagcgaaag ttgggagctg
    19561 gggctttact acatgggtga agaaggctgg ctggaagggg gtgaatccag cgttatcgtt
    19621 ttccgtaacg atgtgaaaga tcgtatcagc atcagccgta cgtctgacgt caacgctgca
    19681 ccgggctacc aaaactttgt tggttttgag acgggcgcta acggacggcg cataccggta
    19741 tttagctact acagcgttaa caaagctcgt attcagggcg tggaaaccga actgaaaatt
    19801 ccgttcaacg atgaatggaa actgtcgatc aactacacct acaacgatgg tcgtgatgtc
    19861 agcaacggcg aaaacaaacc gctatccgat ctgccgttcc atactgctaa cggtacgctg
    19921 gactggaaac cgctggcgct ggaagactgg tcattctatg tttctggtca ctataccggg
    19981 cagaaacgcg ccgacagcgc gacggctaaa acaccgggcg gttataccat ctggaatacc
    20041 ggcgcggcct ggcaggtgac taaagacgtc aaactgcgcg caggcgtgct gaaccttggc
    20101 gacaaggatc tcagtcgtga cgactacagc tataacgaag acggacgtcg ttactttatg
    20161 gcagtggatt atcgcttctg atgagaagat gcccggcgaa ccgggcggac tttcacttca
    20221 gtaaatactg cgcatggaag cgcaggtgat cctctataaa agaggcgatg aagtagtaac
    20281 tgtgatcata tcccggctga atacgcagcg tcatcggcca cgctttctgg cgcgcggctt
    20341 ctgccaatac cgcaggttgc aactggtcgg caagaaactg atcattatcg ccctgatcga
    20401 taagcgtcgg gatcgcatcc tgcgcgttac tggcatacat cagcgcgcaa ctatcccatt
    20461 ccagcatgca tttttgtcct cacctaaata ggtgctaaac gctttgattc cccacgggac
    20521 gctgcacgga ttcacaatgg gcgcaaaggc cgaaacgctg gtgtatttac ccggattttt
    20581 cagcgccata atcagcgcac cgtgaccacc cattgagtga ccgctaatgg cgcagcggtc
    20641 gctgacatta aattgcgact gaaccagcgc cggtaattca tcgcgcagat aatcatacat
    20701 ccggtaatgc gtcgcccacg gcggttgcgt ggcattaaga taaaagcctg cgccctggcc
    20761 taaatcgtag ccatcgtcgt tggcaacctt ttcgccgcgc gggctggtgt ctggcatcac
    20821 cagtacaatc cccagttccg ccgctacccg ctgggcaccc gccttggtgg tgaagttctc
    20881 gtcattgcag gttaatccgg aaagccagta cagcactggt ggcggggtgt gatcacgagg
    20941 ggggggggag aaagatactg aacgtcatcg ggcagtttaa ggtactggag tcgtgtcgcc
    21001 agcgttgctg ccagccttca aaacagcggt gctcttcgag catttccatg cgtggctcct
    21061 tgttgtgttg tttgcaattt cttcatcata ccgtttattc aatgtgctgt gagtaacttt
    21121 cacttccgta tttgcataac gatgttttaa catctgctga tgaaaggcag cggcaattac
    21181 aataattatc gctgtgaata ctggattatg tgcgccgcct cacgcacaat aatcaagctg
    21241 taaatcagct taataacttt gcccccacgc agggtggagg cgtcacacct gcaggagaaa
    21301 tcataaatgc catcactcag taaagaagcg gccctggttc atgaagcgtt agttgcgaga
    21361 ggactggaaa caccgctgcg cccgcccgtg catgaaatgg ataacgaaac gcgcaaaagc
    21421 cttattgctg gtcatatgac cgaaatcatg cagctgctga atctcgacct ggctgatgac
    21481 agtttgatgg aaacgccgca tcgcatcgct aaaatgtatg tcgatgaaat tttctccggt
    21541 ctggattacg ccaatttccc gaaaatcacc ctcattgaaa acaaaatgaa ggtcgatgaa
    21601 atggtcaccg tgcgcgatat cactctgacc agtacctgtg aacaccattt tgttaccatc
    21661 gatggcaaag cgacggtggc ttatatcccg aaagattcgg tgatcggtct gtcaaaaatt
    21721 aaccgtatcg tgcagttctt tgcccagcgt ccgcaggtgc aggaacgtct gacgcagcaa
    21781 attcttattg cgctacaaac gctgctgggc accaataacg tggctgtctc gatcgacgcg
    21841 gtgcattact gcgtgaaggc gcgtggcatc cgcgatgcaa ccagtgccac gacaacgacc
    21901 tctcttggtg gattgttcaa atccagtcag aatacgcgcc acgagtttct gcgcgctgtg
    21961 cgccatcaca actgattaaa aggcaggaac catggagcgc aacgtcacgc tcgattttgt
    22021 tcgcggcgtc gccattctgg ggatcctgct attaaacatc agcgcctttg ggctaccaaa
    22081 ggcggcttat ctcaatcccg cctggtacga tgctattacg ccgcaggatg catggacctg
    22141 ggcatttctc gatctcatcg gccaggtgaa attcctcacg ctttttgcgc tgctgtttgg
    22201 tgctggcctg caaatgttgc tgccccgtgg cagacgctgg atccagtcgc ggttaacgct
    22261 gttagtcttg ttgggcttta ttcacggttt attgttctgg gacggcgata ttctgctggc
    22321 ttacgggctg gtggggcttg gcgttttgct gttattgggg ctgatttccg acagccagac
    22381 cagccgcgcc tggacgccgg atgcatcggc tattttgtat gaaaaatact ggaagcttca
    22441 cggcggcgtt gaagcgatca gtaatcgtgc cgatggtgtt ggcaacagtt tactggcact
    22501 gggcgcacag tatggctggc aactggcagg gatgatgctc attggtgcgg cgctgatgcg
    22561 cagcggctgg ctgaaagggc agttcagctt acgtcactat cgtcgtactg gttttgtgct
    22621 ggtggcgatt ggggtgacca ttaaccttcc tgccatcgcc ctgcaatggc agctggactg
    22681 ggcgtatcgc tggtgtgcct tcttacttca aatgccgcgg gaactgagtg cgccgtttca
    22741 ggcgattggc tatgcgtcgc tgttttatgg cttctggccg caattgagcc gctttaagct
    22801 ggtgcttgcg atcgcctgcg tcggacggat ggcgctgacc aactatctat tgcaaacgct
    22861 gatttgtacc acgctttttt accacctcgg tttgtttatg cattttgacc gcctggagct
    22921 gctggcgttt gttattccgg tatggctggc gaatatcctc ttctctgtta tctggctgcg
    22981 ttacttccgc caggggccgg tggaatggct ctggcgtcag ttaactttgc gtgctgccgg
    23041 accggcaata tctaaaacat caagataacg atctggatta cattcgttaa caaaacggct
    23101 gtaaccgttt ccattgctgt gactcgattc acgaagtcct gtattcagtg ctgacaaaat
    23161 agccgccagc aagcagtcat ttactgcaat ctcataacag gtagtgaata tgatcaccat
    23221 tcgtgatgta gcgcgtcagg ctggcgtctc tgtggcaacg gtttcccggg tgctcaataa
    23281 cagcacgctg gtcagtgccg acacgcgtga agcagtaatg aaagccgtga gtgagctgga
    23341 ttatcggcca aacgccaatg cccaggcgct ggcaactcag gttagcgaca ccattggcgt
    23401 ggtggtgatg gacgtttctg atgcgttttt cggcgcgctg gtaaaagcgg tggatctggt
    23461 cgcccagcag catcagaaat acgtgctaat cggcaatagc tatcatgaag cggaaaaaga
    23521 gcgtcacgcc attgaggtgt taattcgcca gcgttgtaat gcgttgattg ttcactcaaa
    23581 agcattgagt gacgatgaac tggcgcaatt tatggataac attcccggta tggtgttaat
    23641 caaccgcgtt gtgccggggt acgcccatcg ttgcgtttgc ctggataatc tcagcggtgc
    23701 ccgaatggcg acgcgcatgt tgctgaataa cggtcatcaa cgtattggtt atctttcttc
    23761 cagccacggc attgaagatg acgccatgcg taaagcaggc tggatgagtg cgttgaaaga
    23821 gcaggatatt attccgccgg aaagctggat tggcactggt acgccggaca tgccgggcgg
    23881 tgaggcggcg atggttgaac tgctggggcg caatctacaa cttaccgctg tatttgctta
    23941 taacgacaat atggccgctg gcgcactgac agcattaaaa gataatggca ttgcgattcc
    24001 gttacatctc tcaatcatcg gtttcgatga tattcccatc gcccgttaca ccgacccgca
    24061 attaacgacc gtgcgttatc ccattgcttc aatggctaaa ttagccaccg aactggcctt
    24121 gcagggggca gcaggcaata ttgatcctcg tgccagccac tgttttatgc cgacgttagt
    24181 gcgtcgccat tctgtcgcaa cgcgccagaa tgcggcggcg atcactaact caacaaatca
    24241 ggcgatgtaa ccgctttcaa tctgtgagtg atttcacagt atcttaacaa tgtgatagct
    24301 atgattgcac cgttttaatg ttgtaacccg tatgtaacag tgaataatca ctttcaccga
    24361 ggtaatagcg tcataacaac aattaaagcc gttttctgga gcgttaccgg gcatggaaga
    24421 acgaatttta aaaagtgagc ttcggcgttc agtaacactt cattaactct actgccccgc
    24481 cgagcattta tctcaagcac taccctgcat aagaaaaacc ggagatacca tgaataagaa
    24541 ggtgttaacc ctgtctgctg tgatggccag catgttattc ggtgccgctg cacacgctgc
    24601 tgatactcgc attggtgtaa caatctataa gtacgacgat aactttatgt ctgtagtgcg
    24661 caaggctatt gagcaagatg cgaaagccgc gccagatgtt cagctgctga tgaatgattc
    24721 tcagaatgac cagtccaagc agaacgatca gatcgacgta ttgctggcga aaggggtgaa
    24781 ggcactggca atcaacctgg ttgacccggc agctgcgggt acggtgattg agaaagcgcg
    24841 tgggcaaaac gtgccggtgg ttttcttcaa caaagaaccg tctcgtaagg cgctggatag
    24901 ctacgacaaa gcctactacg ttggcactga ctccaaagag tccggcatta ttcagggcga
    24961 tttgattgct aaacactggg cggcgaatca gggttgggat ctgaacaaag acggtcagat
    25021 tcagttcgta ctgctgaaag gtgaaccggg ccatccggat gcagaagcac gtaccactta
    25081 cgtgattaaa gaattgaacg ataaaggcat caaaactgaa cagttacagt tagataccgc
    25141 aatgtgggac accgctcagg cgaaagataa gatggacgcc tggctgtctg gcccgaacgc
    25201 caacaaaatc gaagtggtta tcgccaacaa cgatgcgatg gcaatgggcg cggtagaagc
    25261 actgaaagca cacaacaagt ccagcattcc ggtgtttggc gtcgatgcgc tgccagaagc
    25321 gctggcgctg gtgaaatccg gtgcactggc gggcacagta ctgaacgatg ctaacaacca
    25381 ggcgaaagcg acctttgatc tggcgaaaaa cctggccgat ggcaaaggtg cggctgatgg
    25441 caccaactgg aaaatcgaca acaaagtggt ccgcgtacct tatgttggcg tagataaaga
    25501 caacttggct gaattcagca agaaataaga ctgattgggc gcagtcgttt actgcgccct
    25561 tattgtatgg ccaacaaggt aaaattatat ggtcagctca acgactccgt cttccgggga
    25621 atacttgttg gaaatgagcg gtatcaacaa gtcctttcct ggtgttaagg cacttgataa
    25681 cgttaattta aaagtccggc cacattctat ccatgcatta atgggggaaa acggtgcagg
    25741 aaaatcgaca ttattaaaat gcctgtttgg tatttatcaa aaagactccg gcaccatttt
    25801 attccagggt aaagagatcg atttccattc cgccaaagag gcactggaaa atggtatttc
    25861 gatggtacac caggagttaa acctggtatt acaacgttcg gtgatggata acatgtggct
    25921 ggggcgatat cccaccaaag gcatgtttgt cgatcaggac aaaatgtacc gcgaaaccaa
    25981 agcgattttt gatgaactgg atattgatat cgatccgcgt gcgcgcgtcg gcacattatc
    26041 cgtttcgcaa atgcagatga tcgaaatcgc caaagcgttt tcctataacg cgaaaattgt
    26101 gattatggat gaaccgactt cttcgttaac cgaaaaagag gtcaatcatc tgttcactat
    26161 tattcgtaaa ttaaaagagc gcggctgcgg tattgtttat atctcgcata aaatggaaga
    26221 aatcttccag ttatgtgatg aagttaccgt attgcgcgac ggtcagtgga tcgccaccga
    26281 accgctggca ggactgacga tggacaagat catcgccatg atggttgggc gttctcttaa
    26341 tcagcgtttt cctgacaaag aaaacaagcc gggcgaagtc atcctcgagg tgcgtaacct
    26401 aacgtcactg cgccagccgt cgattcgcga tgtctcgttt gatctgcata aaggggagat
    26461 cctcggtatt gccgggctgg tgggggcgaa acgtaccgat attgttgaga cgttatttgg
    26521 tattcgcgag aaatcggctg gcaccattac gttgcacggc aaacagatca ataaccataa
    26581 tgccaacgaa gccataaacc acggatttgc actggtaact gaggagcgcc gctcaacggg
    26641 aatttatgcc tatctggata ttggttttaa ctcgttaatt tccaatattc gcaactacaa
    26701 aaataaagtt ggtttactgg ataactcgcg gatgaaaagc gatacccagt gggtgattga
    26761 ttcgatgcgg gtaaaaacgc cgggtcatcg gacgcaaatt ggttcgctct ccggtggtaa
    26821 tcagcaaaag gtgattattg gtcgctggct actaacgcaa ccagaaatat taatgctcga
    26881 tgaaccgacg cgcggtattg atgtcggggc gaagtttgaa atttatcagt taattgccga
    26941 actggcgaag aaaggcaagg ggattattat tatctcctct gaaatgcctg agttgttagg
    27001 gataacagac cgtattctgg tcatgagcaa tggtctcgtt tccggaattg tcgatacaaa
    27061 aacaacaacg caaaacgaaa ttctgcgtct tgcgtctttg cacctttaag attaggggct
    27121 tcccatgagt gcgttaaata agaaaagttt tcttacttac ctgaaagagg gcggtattta
    27181 cgtcgttctt ttagttttgc tggcgattat tattttccag gacccaacat ttttaagtct
    27241 gttgaactta agtaatattc tcacccagtc atcggtgcgt attattatcg cgctcggtgt
    27301 ggcagggtta attgtcaccc aggggaccga tctttctgct ggtcgtcagg tagggctggc
    27361 ggcagtggtg gctgcgacat tattgcagtc catggataac gccaacaaag tgttcccgga
    27421 aatggcgacg atgccgattg cgctggttat tctgattgtc tgtgccattg gtgcggtgat
    27481 cggtttgatc aacggtctga ttatcgctta tctcaacgtg acgccgttca ttaccacgct
    27541 cggcacgatg atcatcgtct atggcatcaa ctcgctctat tacgactttg tcggggcgtc
    27601 gccaatttct ggttttgaca gtggcttctc tacctttgct cagggctttg tcgcgctggg
    27661 gagtttccgt ctctcttaca tcaccttcta cgcgttgatt gcggtggcgt tcgtctgggt
    27721 gttgtggaac aaaacccgct tcggtaagaa catttttgcc attggcggta acccggaagc
    27781 ggcaaaaata tctggtgtca acgtcggcct gaacctgctg atgatctacg cgttgtctgg
    27841 cgtgttctat gcctttggcg ggatgttaga agccggacgt atcggctctg ccaccaacaa
    27901 cctcggcttt atgtatgagc tggatgctat cgcggcgtgc gtggtaggcg gtgtatcgtt
    27961 cagcggcggt gtggggacgg tgattggcgt ggtgaccggg gtaattattt ttaccgtcat
    28021 caactatggc ctgacgtata tcggcgtaaa cccatactgg cagtacatca tcaaaggggc
    28081 gattattatc ttcgccgtag cgctggattc actgaaatac gcgcgtaaga agtgatgtga
    28141 ttgcggatat tgccggatgc gtctctggtt ttacaacgtt accgggtgtt ctttctcgcc
    28201 tttcttaaac ttcacttccc cgagctcaat acaacccacc gggcagacgt gaccacaaag
    28261 cagacaaccc acacatttct cggtattaca atgcggtgtg cgggttttct cgctccattc
    28321 catcgcctgg tgaccgccgt cgtaacagga aatataacag cgtccacagc caacacattt
    28381 gtcaagattg atacggggat agacaatata actgcggtct aaatcttccg ccgggacaat
    28441 attgttattc gccagaccta ccatctcctg caacgaatca aatccctgat cggcgagata
    28501 atgcgataac ccgctcgcca tatcttctac gatgcgatag ccgtactgca taatgccggt
    28561 ggtgacctgt aacgttgctg cgccgagcaa taaaaactcc gccgcatctt cccaggtttc
    28621 aatgccgccg ataccgctga ttgggaaatc gcgcagttct ggatgggtgc gcatttgctg
    28681 aatgaagcgc agggcgatcg gttttaccgc tttaccggaa tatccggaaa tactcgattt
    28741 accgttaacg atcggcatac cgattttctg attaagatcg atattggtga tggatttaac
    28801 ggtgttaatt gccgcaatgc catctgcgcc gccgcgcttc gccgccagcg ccacttcgca
    28861 catatcaccg atattcggcg tcatcttcgc cagcattggc agcgtggaac cccgtttcac
    28921 tgcccgacaa tatttttcta ccagctccgg gctttgcccg acatcgctac ccatcgcatg
    28981 agaagtcatt tgcggacagg agaagttaca ctcgatcata tccgcgccag ctgctcccat
    29041 tgctgctcat tttcccccat gatcgaagcg atcaatactt tgtccgggta atcttccttc
    29101 agccgacgca gggcggtcag attctcttcc aacggatgtt cagcaatctg ctccatattt
    29161 ttgaagccga taaaaccggt atcttctttc accagatgat caaaacgcgg cgagacttcg
    29221 ttggcgataa aaaagccgat cgttttaaac accacaccgc cccaacctgt gtcgtaggct
    29281 ttggcacaca tctcatagca gttgcctacc ggcgaagaag agaggcagaa cgggttggga
    29341 aagttcacgc cgcaaaaagt aatcgaaaga tctttcgtta acatgagcaa gctccctcta
    29401 aatagtgatg aatcgcctcg gcggcttctt tcccggtttt cacggcatag accacggttt
    29461 tgtcaccctc aacaatatcg ccagcagcaa agacttgcgg atcgcggatc tgataatttt
    29521 gtgttttgat ggtgttacgc tgctgctcta actccgcaaa ggcatccagt ctggcatgct
    29581 gaccgacggc gagaataatt ttatctgccg ccatcgtcag ttcgcctgat aaccgtacat
    29641 gcttaaacgt gactttattg ccttccacgg ctactggcgt gaatccatcg atgatcgaaa
    29701 cgcccagctc ccgggcgctg gcaaactctt tttcgcttgc cggaaactca tctaactctt
    29761 cacgtgctac gcaagttacc gcctgacagc cgagaacttt cagcgtgctg gctacgtcca
    29821 tcgcgacatc accgccgccg ataattaatg cgctttgtgg aatgctgata tcgccttgcg
    29881 cctgtcgtac acgttgcagg aagtcgacgg caatctcaac gtcgctatgc tcaaacagcg
    29941 gtagcccgga accgcttgat aaccccacgg tgaccagtac cgcgcggttt tccgctttca
    30001 gttgctccag ggtgagtgtt ttaccgattt cgttattgca cttaatggtc acgcccattt
    30061 tttcaatacg ggcgatctct gcatccagca ctgattgcgg taaccggaat tgcggaatac
    30121 cgttacgcaa ccagccaccg gggtgcgctt ctttctcata aatcgtgacg tcataaccct
    30181 ggtttgtcag tgtcacactg gcctgcaatc cggcaggacc tgcgccaata atcgcgactt
    30241 tgccgagcgt tttagtaccg ggctgataaa tttccatccc ggtttgttgt tcaaaatcag
    30301 taacaaaacg ctgtaagcgg ccgatatcaa tgggagcatc aacaccggca cgggtacaac
    30361 cgctttggca taatttctcc gtcgggcaca ctctggcaca aacggcaccg agggcattat
    30421 tttcgcgaat tgtctcggca gcgcctttaa aattacgaaa gtagattgag cgaataaatt
    30481 tccccggatc ggtctgtgcc gggcaagcct gactacaggg agcgtcgtga cataataaac
    30541 agcgagaggc ttctttaatc gccagtaacg gcgtaaaagc cggagtgagt tcatccagat
    30601 aattttgttg cggcatgtga tcttccttaa tgctatgagt aattatggcg aacgctaaat
    30661 aaatattgcg tgctgatatt aaggctatgc gcgtggggaa atttaagctg tgaaaggatt
    30721 cacaaatagc tgcggttcag aatgttatat ccgcagctta ttgagtgaag taataattat
    30781 tctcaataat taacagaaaa gagaaaatat taacgataga aaataagata aacaatcatg
    30841 ccgatagata aaattccgaa gccaatatat gccaccatat aagcgaagaa gagtccggga
    30901 ttttttgctc gccagacata caccggttcc ggtgcatttt taacccgttt atcaatcgcg
    30961 cctgcgcgcc agcctttcca tgcttcgcgg aaacaaaata tcgggataag gaaaaaaacg
    31021 gcaacgacaa aaaactgctg tacatccata actactttcc ttgtttcttt tgtaattcaa
    31081 gtaactgttc gggtgtaacg gcgggatacc cctgcgcatc ttccggcacc tggtgcatag
    31141 ccggaatagg gaccagcggc cctaaaaaac gcggttcacg tttaaaaata taaaggtcag
    31201 ccagcgcacc gaaacgggcg gcaaattcgc gaatacgtac tgacagcata tctttcggtg
    31261 acggtacggc ataacactga gcctgaatcc ccatatgcag cgcaataaat aatgctcgct
    31321 cacagtggaa acgttgggtg ataataatga aatcattagt atcgaaaact ttgcgtgtac
    31381 gcacgatgga atccagcgta cgaaagcctg cgtaatcgag aacaatatct gatgggtcga
    31441 caccagcagc gattaaatct ttgcgcatgg tcatcggctc gttataactt tgcaatgcgt
    31501 tatcgccgct cagtaataga taatttacct taccgctgtt ataggcatta atcgctcctt
    31561 gaatgcggta gcgataatac tgattaataa cgccggtacg ataatatttt gctgtgccga
    31621 gcaccacgcc gacctggcgg taggggagat cctgcaattc gtcgtagata taaggcgcgg
    31681 ttttccagct catccagcga tcgaggccga gcacagtcaa cagcagcaag ccgatcagga
    31741 ctaacaggct gaggaacacg cgctttaaca tgcaggagac tcgagctaag gaaaatttcc
    31801 atcaggctac tttacccgct ggttttgcgc aagaaacggc aaactgtttg ccgggaattt
    31861 cgacaccagg agagggctgg caagccggac aaggcgttca cgccgcatcc ggcaccaggc
    31921 ttaagcgaga agcacgcggt cgatactgtg acagccgaga gctttcagcg ttgcggaggt
    31981 ggcatcccac tgaatcaacg gcgcatcggc tttttctgcc agaaccgcgc gctggatatc
    32041 cgggtaatca taacccttga gattcaacag aattaacgcc ccctgcaacg gcggcagagt
    32101 cgggttgaat gcggcgtttt cagcgtagct gccgctgaaa atacgaccgt ctttacattc
    32161 cagcgcgaca ccgcttggtg acttactgta aggcatgtgc gaacggtttg ccgctgcaat
    32221 cgccgcctga gaaagcgcat caccagtcag cgcatagccg tgatcctgct cgtccatcag
    32281 cagcgtttta atctccagat ctttcggccc aaaggcatcc ggcagatagt cacgcagcgc
    32341 gtgtgcctcg cggcccggca aatgaatacg cagatccaga ccgctgttca gttcattcat
    32401 aaactgacgg cagtgaccac aaggcgtata gttaacggtg atggctgcaa gcgctttttc
    32461 accactcaac caagcgtggc tgatcgcgct ttgttcggca tgaacggttt gctgcattgt
    32521 tgcaccaata aattccatat tggcaccaaa ataccaggtt ccgctcacac cgcgcgcaat
    32581 tgcgccaaca ttaaaattcg acaatggcgt acgcgcacag gccgccgcca gcggaagtag
    32641 tgcgaatgcc agcgcgtctt cgtccagtcc cgttgcgctc ttcagcgatg agacttgctc
    32701 cccggtcaac aaagcgggga agtacttgtc tgccagaata ggttccagtg cagattgcaa
    32761 gttatccgca agttgggcaa aagcggtttg aaaacgtgga tgcatggcgt tgcctcataa
    32821 tcatgtaatg ggataacagt ttacgaaccc gttatggctt tatatgtgat ctgaatctca
    32881 ttaattatgc aacacgtgaa acttacatca aaaatgcgcg acgtatcgca aattttagcc
    32941 cattactgcc agaataatcg ggaaaaggaa cggtgcgatc agcgaagtaa ttatcccgca
    33001 taacaccagc gctagcgaac taaatgcacc ttcctgataa tccagctcgg cgcagcgcgc
    33061 cgtaccgagg gcgtgcgagg cagtccccat tgccagaccg cgcgcagctt tggtacgaat
    33121 acgcatcgca ttaagcaatg tatggccaaa taccgcgccg aggatgccga cgaaaatcac
    33181 gcaaactgcg ctgattgccg gaataccgcc aatactgccg ccaaccgcca ttgcaatagg
    33241 cgtggtgact gatttcggca ggattgacgc ggcgatttcc ggtgaagcgc ccatcaacaa
    33301 tgccacggaa gtccccgtca ccatcgcaac cacgctgccg ataaaacaga tggtgatgat
    33361 cgatttccag cgcgcgcgga tctggtgtag ctgctcatat aaaggatagg ccagcgcgac
    33421 gaccgccggt tgcagcagat cgtttagcac ttcgctacct ttaaagtaac tgtcgtaaga
    33481 gatgccggtc agcattaaaa aaggaatgat caccaccatt gctaccagca acgggttaag
    33541 caggggaaac ttataccgtg ccgccagttt gcgggcggca aaaaagacaa tcaacgtcag
    33601 cggtaatgac caccagatat tcgccatcat tcttctgatc ctttctgacc tacgacttta
    33661 cgttcaccgt gtaccagttg tgaactccag ctcaccacca gaaaaaccac cagcgtactg
    33721 acagcacagg aaaccactac cgggccaaac tgcgcgcgga gcaaatcaaa atattgcatg
    33781 acgcctacgc caatcggcac aaacaatagc gccatatagc gaatcagtac gtagcacccc
    33841 ggattgaccc atttcgccgg aagaatttgc aaggccagca ggacaaacag gatcagcatc
    33901 ccgatgatgc tgcccggaat ggttaccggt agcagggaag caatgaaaat gcctgcatac
    33961 aggcaggcat aaatcaggac gaaagcgcgt aaatattgcc agataatgtt cagtgtctta
    34021 ctcataatca acaccccaga tgagatggat tcatcataca attaattcgt aaaacgtgct
    34081 accgatcaca ttaagaaatc agcgcttatt ttttcgctac ccggtattac gcaatgagca
    34141 gcgaactcag gcatacatca agcatagaga gggattgcag tttgcagcaa tccctctttg
    34201 cagaccttta atccgctgac cataggcgct aacatagcgc ctcatttttt tgcgggtgat
    34261 gatatgcgtg tgttactggc accgatggag ggagtgcttg actctctggt gcgtgaattg
    34321 ctgaccgaag ttaacgacta cgatctgtgc atcaccgagt ttgtccgcgt ggtggatcaa
    34381 ctgctgccgg taaaagtctt tcatcgcatt tgccctgagc tacaaaacgc cagccggaca
    34441 ccatctggta cgctggtgcg cgtgcagttg ttaggtcagt tcccacaatg gctggcagag
    34501 aacgccgccc gtgcggtgga gttaggtttc tggggcgtgg atctcaattg cggctgcccg
    34561 tcgaaaacgg ttaacggtag cggcggcggg gcgacgttac tcaaagatcc tgaactcatc
    34621 taccaggggg caaaagcgat gcgtgaagct gtaccggcgc atttgcctgt cagcgtgaaa
    34681 gtgcgtctgg gctgggacag cggtgagaag aaatttgaaa tcgccgatgc ggttcaacag
    34741 gctggcgcta cggagctggt ggtgcatggg cggacgaaag agcagggtta ccgcgcggag
    34801 catattgact ggcaggcgat tggcgagatt cgccagcggc tgaatattcc ggtgattgcc
    34861 aacggtgaaa tctgggactg gcagagcgcg caacaatgca tggcgatcag cggctgcgac
    34921 gcagtgatga ttggtcgcgg ggcgctcaat attcccaacc tgagccgggt ggtaaaatat
    34981 aacgaaccgc gaatgccgtg gccggaggtg gttgctttgc tgcaaaaata tacccgtctg
    35041 gaaaagcagg gcgataccgg gttatatcac gttgcgcgga ttaaacagtg gttgagttat
    35101 ttgcgtaaag aatacgatga agcaacggaa ttatttcagc atgttcgggt gttgaataac
    35161 tcccctgata ttgcaagggc tattcaggca attgatatcg agaaactcta aaaaccatag
    35221 tgatgttttt gacaatgtgt tagctcttta tcaataggcc gcacggttgt ttggtggcgt
    35281 caaaatcgcc gaggcgttcc ctgaaggccg gggcagccca catggatgtg ggctgagggc
    35341 gcgttttaca gggatgttac ctcgcgcccg acccggtagc cgtaagggat aagccgaggg
    35401 caccgcgcag cggcgatttt gttcgccaga gcccgggggg gcagggggcg gcggcgattg
    35461 gtcgccccct gcgcgctcct tgcgccagtg attatatgaa gcaatgcaca tgacaggagc
    35521 gcaacaggat gagagactga aatattctcg ttattttcgg atctgatgac attaattaat
    35581 tttaaaatat catcttaaat acgccagtca ccaccagtag cccaatcagg aaaataatca
    35641 atacagccca gagtataatt ttcattggcg ttcctttatt tgtgagtgct gaataagtat
    35701 aggaaaaaac gaagcccttt gccgttatcc ataaacgatt aagataaatc ggaagatatc
    35761 atttacttag tctatatttt tctgggtatg tcgttttatt gctactgatg tattgccacg
    35821 cgaaactaaa aaatatatcg caacacacgg taatctccgc gcaccgtttc ttacctgatt
    35881 attcccccat gaatcgtgat tccttttatc cagccatcgc ctgttttccg ctgttactga
    35941 tgctggccgg gtgtgcgcct atgcatgaaa cccgccaggc gttaagccag caaacgcccg
    36001 ctgcacaagt tgacaccgca ttacccacgg cgctgaaaaa tggttggcca gacagccaat
    36061 ggtggctgga gtatcacgat aatcagctca cttcattaat taacaatgca ctacagaacg
    36121 caccggatat gcaggtggct gaacagcgca tttagctagc tgaagcccag gcgaaggcag
    36181 ttgccactca ggatggtccg cagatcgact tttcggcgga tatggagcgg caaaaaatgt
    36241 cggcagaagg gctaatgggg ccgtttgctc tgaacgatcc ggccgcaggt acgaccggtc
    36301 cgtggtacac caacggaact tttggcttaa cggcgggctg gcatctcgat atctggggaa
    36361 agaatcgggc ggaggttact gcccgcctgg gtacggttaa agcacgggcg gcggaacgcg
    36421 agcaaacccg ccaattgctg gctggcagcg tagcccgcct gtactgggag tggcaaaccc
    36481 aggcggcgtt aaacacggtc ttgcagcaaa tagaaaaaga gcagaacacc attatcgcga
    36541 ccgatcgcca gctatatcag aacgggatta cttcttcagt tgacggtgtg gaaaccgata
    36601 ttaatgccag caaaacccgg cagcagctca acgatgtcgc ggggaaaatg aaaattattg
    36661 aggcacggtt aaatgcactc acaaatcacc agactaagtc attgaagctt aaaccggtcg
    36721 cgttgccgaa agtggcaagc cagcttcctg atgaactggg gtactcctta ctggcccggc
    36781 gggcagattt gcaggcggcg cactggtacg ttgagtcatc gttaagcact attgatgcgg
    36841 caaaagcggc attttatcct gacatcaacc tgatggcctt cctgcaacag gatgcgttgc
    36901 acttaagcga tctgttccgt cattccgcgc agcaaatggg cgttacggca ggcctgacgc
    36961 tacctatttt cgatagtggt cgtcttaacg ccaatctcga tatcgcaaaa gccgaaagca
    37021 acttgtctat cgccagctac aacaaagcgg tggttgaagc agtgaatgac gtggcgcggg
    37081 cagccagtca ggttcagaca ctggcggaga aaaaccagca tcaggcgcaa attgagcgcg
    37141 atgccttgcg tgtggtaggt cttgcgcagg cccgctttaa cgcgggcatc attgctggtt
    37201 cccgcgtcag cgaagccaga atcccggcgc tgcgtgagcg ggccaatggc ctgttattgc
    37261 aagggcagtg gctggatgcc tccattcgac tcaccggtgc gctgggcggg ggttacaaac
    37321 gctgatgata tgcgccttct atacttaacg tttattcagc gttaagtgga gaactcgatg
    37381 gcataggtcg cgataattac cgcttccgat tcggggatcg gcaaagagtg cgcgttatta
    37441 ctggcgcagc aggggtttga tattggcatt acctggcact cagatgaaga aggggcaaaa
    37501 gataccgcgc gtgaggtggt tacccacggc gtacgtgcgg agatcgtgca actggatctc
    37561 ggcaatctgc cagaaggtgc gcaggcgctg gagacactca tccagcgatt tgggcgtatt
    37621 gatgtgctgg tgaataatgc gggtgcaatg accaaagcgc cgtttcttga tatggctttt
    37681 gatgagtggc gcaagatttt taccgttgat gttgatgttg atggtgcatt tttatgctcg
    37741 caaattgcgg ctcgtcagat ggtgaaataa gggcagggcg gtcgcatcat caacattacg
    37801 tcggtacatg aacatacgcc gctgccggat gccagcgcct acacagccgc taaacatgcg
    37861 ctcggcgggt taaccaaaac gatggcgctg gagttggtca ggcataagat tttggtgaac
    37921 gcagtcgcgc ctggggcgat agccacgcca atgaacagca tggatgatag cgacgtgaag
    37981 cccgacgcgg agccttcgat tcctctacgg cgttttggcg caacgcatga gattgccagc
    38041 ctggtggtgt ggctttgttc ggagggcgca aattacacga cagggcagtc gttgatagtg
    38101 gatggcggct ttatgttggc gaatccgcag ttcaacccag aatagcgcag tgaggtgcgt
    38161 ggatatttca atgggttagg ccagataaga caccagcgtc gcatcaggca tggaacaccc
    38221 ggtgccggat gtggagtgaa tgcttatctg tggcaactgc tttacgcctg attatccggc
    38281 tttttcttcc cgcgccgttt cagccaccag cgcacgccca ccaccagaac cacgaccaga
    38341 atcaaccaga cccagtgctt caaatgctgg tcgagattgt gcaaccacgg cgcaatcacc
    38401 tgaccaccag cgtaaccaat agtggtaaaa atcaacgccc aggcaaatgc gccgagaata
    38461 ttcagcggca gaaagatttt cggcggcagc tggctggcac caatcagcgt cgggccaatc
    38521 acccgaaagc catacataaa gcgcgtacca atgacaaaca gatacggatg gcgttggata
    38581 agtttctgtg cccgctcaat tttatcctga tgtttcgaga aacggcgtaa cagcttgccg
    38641 ccaaaccgcc gcccgcacag atagagcacc tggtcgccaa tcatgccacc aagcgccaca
    38701 gaaagtacca ccagtgggaa ctttaatagc ccctgatgtg ccgcaacgcc tcccagcaaa
    38761 gtcacggttt caccttccgc caggctaccg ataaccagcg cggcataacc atattgtgag
    38821 atgagtgtat tgagatccat aggccagttt gtctcccttt ggttgcctgt aagcatacac
    38881 cctgtgaatc attcacgctg aaaaaggaca tacatcagac ggtgcaaatt acatggatga
    38941 acaaaaaata atcacaagct ggcttatact tgaactgtca ggttcatgaa ctcacaacaa
    39001 gggggcggta tgagccatgt ctggggactg ttttcccatc ccgatcgtga aatgcaggtg
    39061 attaatcgcg aaaacgaaac gatttctcac cattacaccc accacgtttt gctgatggcg
    39121 gcgattccgg tgatttgcgc cttcattggc actacacaga ttggctggaa ttttggcgat
    39181 ggcactatcc tgaagttatc ctggtttacc ggactggcgc tggctgtctt gttttacggc
    39241 gttatgctgg ctggggttgc ggtgatgggg cgggtcatct ggtggatggc gcgcaattac
    39301 ccgcagcgtc cgtctcttgc gcactgcatg gtcttcgcgg gctacgttgc aactccgctg
    39361 tttttaagcg gtctggtggc gctttatcca ctggtctggt tgtgtgcgct ggtcggcacg
    39421 gtggcgctgt tttacaccgg ctacctgctt taccttggta ttccgagctt cctgaatatc
    39481 aacaaagagg aaggccttag cttctccagt tcgacgctgg cgattggcgt gctggtactg
    39541 gaagtgctgc tcaccctaac cgttatacta tggggctacg gttatcgttt gttctagaaa
    39601 gttctttgac gttgcattgc tggcgtaaat gtcagcaatg cagcatttac tcacgattct
    39661 cctttgacga tcgcactttt tgctcgttat gatggcgtcg ctagcctcag taaatcctta
    39721 ctccggcggt gcgcaacccg tgcgcgtgaa ccactatctg aatgctcatc atgccgaaat
    39781 ttcgagtttc tttatttagc ttggccctga tgctggctgt gccttttgca ccgcaggccg
    39841 ttgcgaaaac ggcagccgct accaccgctt cacaaccgga aattgcctcc ggtagcgcga
    39901 tgattgttga tctgaatacc aacaaagtga tctattcgaa ccacccggat ctggtgcgtc
    39961 cgattgcgtc tatcagcaaa ttaatgaccg cgatggttgt gctggatgca cgactgccgc
    40021 tggatgaaaa actaaaagtg gatatcagcc agacgccgga gatgaaaggg gtctattcgc
    40081 gcgtacgact gaatagcgaa atcagccgta aagatatgct gttgctggcg ctgatgtctt
    40141 cagaaaaccg cgcggcggca agccttgcgc accattatcc cggtggttac aaagccttta
    40201 ttaaggcaat gaatgcgaaa gcgaaatcgc tcggaatgaa caacacgcgc tttgttgaac
    40261 ctaccggatt gtcggtgcat aacgtttcaa ctgcccgtga cttaaccaaa ctgctcattg
    40321 ccagcaaaca atatccgttg atcgggcagt taagtactac ccgggaagat atggcaacct
    40381 tctctaaccc gacgtatacg ctgccgttcc gcaatactaa tcatctggtg tatcgcgata
    40441 actggaatat tcagttaacc aaaaccggct ttaccaatgc ggcgggccat tgtctggtga
    40501 tgcgtacggt tatcaataat aaaccggtgg cgctggtagt gatggacgcg tttggcaaat
    40561 atacccattt tgccgatgcc agccgcctgc gtacctggat tgaaaccggt aaagtgatgc
    40621 ctgtgccggc agcagcgttg agctataaaa aacaaaaagc cgcccaaatg gcggcggcgg
    40681 ggcagacggc acagaacgat taagtgaatt cggatggcga tactctgcca tccgtaattt
    40741 ttactccact tcctgccagt ttttccgttt actggtttta ccgatccccg gattcatgct
    40801 attggtcgga tcattctcgc gatagaactt ctgcaacgtc tccggtgctt tatacaaatg
    40861 accgacgtta tgctcggcag ggtactgcgc gccgcgctgc tgtagcagtt ccagcatctg
    40921 ttcttttaac gcatgcacat ccacgccttt tttcactatg taatcctgat ggaagacata
    40981 gcacataaaa tggccgtaat agagcttgtg caccagctgg ctgtcgatct ccggcggtaa
    41041 atgctcatac cactcggtgt cgttacgccg cagagcgata tccagtgcca gaatgtcttc
    41101 gacttcatcg gaatgcaccg cctgataacg aattgctgcg cccgcagcgg cgaaacggtg
    41161 taaaaacgct ttgctacctt cctccggcgt acagacaaag aaatcgccct cggcctgttt
    41221 gaaataatcc accagccacg atttggcttc accgacgcca tcgcctgcca tttttaacag
    41281 cagatgatgc tcgtatttat cgcgccagtt tttcatgcgc ggcggtaaat ggctggggaa
    41341 caggtgaccg aatttttgca ttgcacggtc agtaaaatgc ggacggaaga atttcacttt
    41401 ctccagcatc gcatcggtgc gtcccttgag attaaagaag aacggcatct tgtcggtgcc
    41461 taacttatca atcatcagga aggtgtcttt gccgtatttt tccgcaatat cgtagatatc
    41521 ccggtgcata tattccccgg caaccggcag attttcgaag ttagccagaa tatgacggcg
    41581 gatttcggtc agcacttccg gctggttggt gccgatataa aacacctgct gatttttttc
    41641 cgcttcgaag gtatcaagac gtaccgcaaa gacggccagc ttcccggcgc aaccagaaga
    41701 ttcaaataac cgatccggat cggcgttata acgtgcgggc gtgtcggctt caatatcacg
    41761 aacgcggtgg acatagtcat aatcgtgggc gtgacgacca tcgtgacgca catcgtcatc
    41821 tttgatgcga tcgtcatcca gcttgctaag gatctgctcc ggcgtttcgc ccagatcaat
    41881 ccccagatgg ttcaccagcg tcagtttgcc atcttcatct atacgcgcga ataacgacat
    41941 ttcggtatac gccgggccac gttgcaccag cgagccgccg gagttgttac aaataccgcc
    42001 gatgaccgat gcgcctatac acgatgatcc aatcaccgaa tgcggttcgc gtcccagcgg
    42061 tttgagggct ttttccagcg aatagagcgt ggtgcccgga taggccagca cctgttcgcc
    42121 cttgccgaga acgtgcagct tgtcgagacg cagggtgctg atgatcacga tatcgcgatc
    42181 atagtcgtta ccgtttggcg tcgatccttc ggtcaggcct gtattggcag cctgcatcag
    42241 aataattttg tcggcggtga cgcaggcttt cagcacccgc cacaattcca gtagtgagcc
    42301 agggaaaacg acagccagcg cgtcgccctg accagaacgg aagcccttgc gatagcgggc
    42361 cgtttttgcg ggatcggtga gcaggtgtga atgaccgacc agacgggcaa gttcattcaa
    42421 aaaggcttta ttatcagttg ttgtcatgga agacattttc cactccttgt ggtggcgaaa
    42481 aaatatcaca aactagaata gcgccttgta tgacaattca gcgagtgaat attttgaaaa
    42541 ttcagacaat aaactgagca ttctttttct ctctccatca tgcttatggc aaactacggt
    42601 ttttgcacca ttttcgctgc cacgtcgggc aacaaaggaa gaaaaatcca tatgaaatgg
    42661 ctatgttcag taggaatcgc ggtgagtctg gtcctgcagc cagcactggc ggatgattta
    42721 ttcggtaacc atccattaac gcctgaagcg cgggatgcgt tcgtcaccga tctgctcaag
    42781 aaaatgacgg ttgatgagaa aattggtcag ctgcgcttaa tcagcgtagg cccggataat
    42841 ccgaaagagg cgatccgcga gatgatcaaa gacggtcagg ttggggcgat tttcaacacc
    42901 gtaacccgtc aggatatccg cgccatgcag gatcaggtga tggaattaag ccgcctgaaa
    42961 attcctcttt tctttgctta cgacgtgctg cacggtcagc gcacggtgtt cccgattagc
    43021 ctcggtctgg cctcgtcttt taacctcgat gcggtgaaaa cggtcgggcg tgtctctgct
    43081 tatgaagcgg cagatgatgg cctgaatatg acctgggcac cgatggtcga tgtctcgcgc
    43141 gatccgcgct ggggacgtgc ttccgaaggt tttggcgaag atacgtatct cacctcaata
    43201 atgggtaaaa ccatggtgga agcgatgcag ggtaaaagcc cggcagatcg ttactcggtg
    43261 atgaccagcg tcaaacactt tgccgcatac ggcgcggtag aaggcggtaa agagtacaac
    43321 accgtcgata tgagtccgca gcgcctgttt aatgattata tgccgccgta caaagcgggg
    43381 ctggacgcag gcagcggcgc ggtgatggtg gcgctgaact cgctgaacgg cacgccagcc
    43441 acctccgatt cctggctgct gaaagatgtt ctgcgcgacc agtggggctt taaaggcatc
    43501 accgtttccg atcacggtgc aatcaaagag ctgattaaac atggcacggc ggcagacccg
    43561 gaagatgcgg tgcgcgtggc gctgaaatcc ggaatcaaca tgagtatgag cgacgagtac
    43621 tactcgaagt atctgcctgg gttgattaaa tccggcaaag tgaccatggc agagctggac
    43681 gatgctgccc gccatgtact gaacgttaaa tatgatatgg ggttgtttaa cgacccatac
    43741 agccatttgg ggccgaaaga gtctgacccg gtggatacca atgccgaaag ccgcctgcac
    43801 cgtaaagaag cgcgtgaagt ggcgcgcgaa agcctggtgt tgctgaaaaa ccgtctcgaa
    43861 acgttaccgc tgaaaaaatc ggccaccatt gcggtggttg ggccactggc ggacagtaaa
    43921 cgtgacgtga tgggcagctg gtccgcagcc ggtgttgccg atcaatccgt gaccgtactg
    43981 accgggatta aaaattccgt cggtgaaaac ggtaaagtgc tgtatgccaa aggggcgaac
    44041 gttaccagtg acaaaggcat tatcgatttc ctgaatcagt atgaagaagc ggtcaaagtc
    44101 gatccgcgtt cgccgcaaga gatgattgat gaagcggtgc agacggcgaa acaatctgat
    44161 gtggtggtgg ctgtagtcgg tgaagcacag gggatggcgc acgaagcctc cagccggacc
    44221 gatatcacta ttccgcaagg ccaacgtgac ttgattgcgg cgctgaaagc caccggtaaa
    44281 ccgctggtgc tggtgctgat gaacgggcgt ccgctggcgc tggtgaaaga agatcagcag
    44341 gctgatgcga ttctggaaac ctggtttgcg gggactgaag gcggtaatgc aattgccgat
    44401 gtattgtttg gcgattacaa cccgtccggc aagctgccaa tttccttccc gcgttctgtc
    44461 gggcagatcc cggtgtacta cagccatctg aacaccggtc gtccgtataa tgccgacaag
    44521 ccgaacaaat atacttcgcg ttattttgat gaagctaacg gagcgcttta tccgttcggc
    44581 tatggtctga gctataccac tttcaccgtc tctgatgtga aactttctgc gccgaccatg
    44641 aagcgtgacg gcaaagtgac ggccagcgtg caggtgacga acaccggtaa gcgcgagggg
    44701 gccacggtag tgcagatgta cttgcaggat gtgacggctt ccatgagtcg ccctgtgaaa
    44761 cagctgaaag gctttgagaa aatcaccctg aaaccgggcg aaactcagac tgtcagcttc
    44821 ccgatcgata ttgaggcgct gaagttctgg aatcaacaga tgaaatatga cgccgagcct
    44881 ggcaagttca atgtatttat cggcactgat tccgcacgcg ttaagaaagg cgagtttgag
    44941 ttgctgtaat gctttagtaa gggcgcgacg tttgcgccct ttgcctgatg ttcatatctt
    45001 aacttgctga attttcgccc tttgtaggcc ggataaggcg ctcgcgccgc atccggcagg
    45061 aaacgccgga ccgaatatgc ctcttaagta tttatctgat tctgcaactg aatccttccg
    45121 ctcaggctaa ctctgtcatt atcaactatg cttttctctt aattcgctga aaacaacagt
    45181 aaaagaatga ggaaagcatc atgccactct taaagctctg ggcaggttca ctggttatgt
    45241 tggcagccgt gagcctgccg ctacaggcgg cttcccccgt taaagtcggt tcaaaaatcg
    45301 ataccgaagg cgcgctgctc ggcaacatca tcttgcaggt actcgaaagc cacggcgtac
    45361 cgacggtgaa taaagtgcaa ctcggaacga ctcctgtggg gcgcggggcg attacttccg
    45421 atgaactgga tatctatccg gaatataccg gcaatggcgc tttcttcttt aaagatgaaa
    45481 acgatgcagc gtggaaaaat gccgggcagg gctacgagaa agtcaaaaag ctcgatgcgg
    45541 agcacaacaa gttaatctgg ctgacgcccg cgcctgcaaa taacacctgg actatcgccg
    45601 tgcgtcagga tgtggcagag aaaaacaaac tcacttcgct tgccgacctg agtcgttatc
    45661 tgcaagaggg cggcaccttc aaactggcag cctcggcaga gtttatcgaa cgcgccgatg
    45721 cgttacccac gtttgaaaaa gcctatggct ttaagctcgg tcaggatcag ttgctgtcac
    45781 tggctggtgg cgacacggcg gtgacgatta aagccgctgc ccagcaaacc tctggcgtta
    45841 atgccgcaat ggcttacggc actgacggtc cggtcgcggc gctggggctg caaaccttaa
    45901 gcgatccgca aggcgtgcaa cctatctacg cgcctgcacc agtggtgcgt gaatcggtgt
    45961 tgaaggagta tccgcaaatg gcacagtggc tacagccagt cttcgccagc ctcgatgcaa
    46021 aaacattgca gcaactgaat gccagcattg cagtggaagg actggatgcc aaaaaagtgg
    46081 ctgccgacta cctgaaacaa aaagggtggg cgaagtaatt tcccgtgact tatctacgta
    46141 ttaatcctgt tctggcgctg ctgttgttgc tgacggcaat cgcagcggcg ctgccgttta
    46201 tcagttacgc gcctaatcgt ttagtcccgg gtgaagggct tcatctctgg caactgtggc
    46261 cgcaaacgat ctggatgctg gtgggcgttg gttgcgcctg gctgacagcc tgttttgtcc
    46321 ccgctaaaaa aggcagcatt tttgcactca ttctggcgca attcgtcttc gtattgttgg
    46381 tgtggggagc tggaaaggcg gcgacccaac tggcgcaaaa tggcagtgcg ctggcgcgta
    46441 ccagcctcgg cagaggtttc tggctggctg cggcgctggc attgctggcc tgtagcgatg
    46501 ccatccgccg aatctccacg catccgctgt ggcgctggtt gttgcatatg cagattgcca
    46561 ttattccgct gtggttgctg tactccggca cgcttaacga tctctcacta atgaaagaat
    46621 acgccaaccg tcaggatgtg tttgacgacg cgctggcaca acatctgacg ttgctgtttg
    46681 gtgcggtgct gcctgcgtta gtgattggtg tgccgttggg catctggtgc tacttttcca
    46741 ctgcgcggca gggggcaatt ttttctctgc tcaatgtcat tcagaccgtg ccttcggtgg
    46801 cgctctttgg cctgttgatt gcgccgcttg ccgcgctggt tacggccttt ccgtggctgg
    46861 ggaagctcgg catagcagga accggaatga cacccgcact gattgcgctg gtgctctatg
    46921 ccttgctgcc gctggtgcga ggcgtggtag tcggtttgaa ccagatcccg cgcgatgtgc
    46981 tggagagcgc cagagcgatg gggatgagcg gggcgcagcg tttcctgcat gttcagttac
    47041 cgctggcgtt accggtattt ctgcgcagcc tgcgggtggt gatggtgcaa actgtaggta
    47101 tggcggtgat tgcggcgtta atcggcgcag gcggttttgg tgcgctggtt ttccaggggc
    47161 tgctaagcag cgccattgat ttagtgttgc tgggggtgat cccggtaatt gttctggcgg
    47221 tgcttaccga cgcgctgttc gatttgctta tcgcactgct gaaggtgaaa cgtaatgatt
    47281 gaatttagcc atgtcagcaa actgttcggc gcacaaaaag ccgttaacga tctcaatctc
    47341 aattttcagg aagggagttt ttcggtgctg attggcacat ctggctccgg caaatccacc
    47401 accctgaaaa tgattaaccg cctggtggag catgacagcg gagtgatccg ctttgccgga
    47461 gaagaaattc gctcgctgcc agtactggag ttgcgccgcc ggatgggcta tgccattcaa
    47521 tctattggcc tgttccccca ctggagcgtg gcgcaaaaca tcgctaccgt gccgcaatta
    47581 caaaaatggt cgcgggcgcg gattgacgat cgtatcgacg aattaatggc gctactgggg
    47641 ctggagtcaa atttgcgtga gcgttatccg catcagcttt ccggtggtca gcagcaacgt
    47701 gtgggagtgg cgcgtgcact ggctgccgat ccgcaagtct tactgatgga tgaacctttt
    47761 ggcgcactgg acccggtaac gcgcggcgcg ttgcaacaag agatgacgcg cattcaccgt
    47821 ttgctggggc gcactattgt gctggtcact catgatattg atgaggcgct acggctggca
    47881 gaacatctgg tattgatgga tcacggtgaa gtggtgcagc aggggaatcc gctgacgatg
    47941 ctgactcgtc cggcgaatga ttttgtccgc cagttttttg gacgtagtga actgggggtg
    48001 cacctgcttt cgttacgtag cgtggcggat tacgtgcgtc gcgaagaacg ggcagaaggt
    48061 gaggcactgg cagaagagat gacgctacgc gatgcgctct ccctgtttgt cgcgcgggga
    48121 tgcgaggtgc tgccggtggt gaacacgcag ggccagcctt gcggcacgct gcattttcag
    48181 gatctgctgg tggaggcgta agcgtatgaa gatgttgcgc gatccgctgt tctggctcat
    48241 tgctcttttt gtggcgctga ttttctggct gccttacagc cagccgctgt ttgctgcctt
    48301 gttcccacaa ctgccacgac ccgtttatca gcaagaaagt tttgcagctc tggcactggc
    48361 tcatttctgg ctggtgggaa tttcgagttt gtttgcggtg atcattggca caggtgccgg
    48421 aattgctgtt actcgcccgt ggggcgcgga atttcgccca ctggtggaaa ctattgccgc
    48481 cgttggacag acttttccgc ctgtcgcagt gctggcgatt gccgttccgg tgatcggctt
    48541 tggtctgcaa ccagcgatta tcgccttgat cctttacggt gtgctgccca tcctgcaggt
    48601 gacacttgcc gggctgggag cgattgatcc cagcgtgaca gaagttgcga aaggtatggg
    48661 aatgagtcga ggtcagcgac tgcgtaaggt cgagctaccg ctggcggctc cggtgattct
    48721 ggcgggcgtg cgaacttcgg tgattatcaa cattggtacg gcgacgatcg cctcaacggt
    48781 aggggccagt acgctgggta cgcccatcat catcgggctt agcggattta ataccgcgta
    48841 tgtgatccag ggggcgttac tggtggcact ggcggcgatc atcgcagacc gcctgtttga
    48901 aaggctggtg caggcgctta gccagcacgc aaaataaagg tataacctgc gagcatgacg
    48961 ccaccaattc cgcctaacgc cataaacagg aacagggcga tgaccccaat tttagctatg
    49021 cgcataatgc actccttatg ttaacgaaag gattgtacag taaagcgcat ttgttaacga
    49081 atcattaaat accgagtggg aaaatatcat ggccttgttc ttgccaactg gtgagttgct
    49141 gctgttgggc ggaggttcga ttttcaccgc accacaccag caatgtacgg ccttcgaata
    49201 gttcagggcg tagttgattg agcgagtggg cgagcgagga catcaatgcg ccatccttgt
    49261 tgactggcaa tccagccctc cagccacaga cgggtggtat cctgaatatt ccagccaacc
    49321 accagcgcat ctttaccctg ttttttacgt gccgaagcca gacaaatggc gatgtagttg
    49381 atcagtacgc cgtcgaggat cgccagcagc gcctggagag tcggttgttg gcactgaagc
    49441 cgtcggcgca gaggaataaa cagatgtgtg gtgagtgtct gggcggggta atcctgaccg
    49501 cgctctttga tccacgttcg caggctatgt agattgc
//
LOCUS       DAJTSA010000021        48328 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000021 DAJTSA010000000
VERSION     DAJTSA010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.021"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            188..481
                     /locus_tag="OO369_001278"
     CDS             188..481
                     /locus_tag="OO369_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5981616.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(522..1712)
                     /gene="nepI"
                     /locus_tag="OO369_001279"
     CDS             complement(522..1712)
                     /gene="nepI"
                     /locus_tag="OO369_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR5981617.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1923..2375)
                     /locus_tag="OO369_001280"
     CDS             complement(1923..2375)
                     /locus_tag="OO369_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR5981618.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2428..3762)
                     /gene="adeQ"
                     /locus_tag="OO369_001281"
                     /gene_synonym="yicO"
     CDS             complement(2428..3762)
                     /gene="adeQ"
                     /locus_tag="OO369_001281"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR5981619.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3937..5703
                     /gene="adeD"
                     /locus_tag="OO369_001282"
     CDS             3937..5703
                     /gene="adeD"
                     /locus_tag="OO369_001282"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR5981620.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5749..7140)
                     /gene="uhpT"
                     /locus_tag="OO369_001283"
     CDS             complement(5749..7140)
                     /gene="uhpT"
                     /locus_tag="OO369_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR5981621.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7278..8597)
                     /gene="uhpC"
                     /locus_tag="OO369_001284"
     CDS             complement(7278..8597)
                     /gene="uhpC"
                     /locus_tag="OO369_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR5981622.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8607..10109)
                     /gene="uhpB"
                     /locus_tag="OO369_001285"
     CDS             complement(8607..10109)
                     /gene="uhpB"
                     /locus_tag="OO369_001285"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR5981623.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10109..10699)
                     /gene="uhpA"
                     /locus_tag="OO369_001286"
     CDS             complement(10109..10699)
                     /gene="uhpA"
                     /locus_tag="OO369_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR5981624.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10774..11064)
                     /gene="ilvN"
                     /locus_tag="OO369_001287"
     CDS             complement(10774..11064)
                     /gene="ilvN"
                     /locus_tag="OO369_001287"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5981625.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11068..12756)
                     /gene="ilvB"
                     /locus_tag="OO369_001288"
     CDS             complement(11068..12756)
                     /gene="ilvB"
                     /locus_tag="OO369_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR5981626.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12862..12960)
                     /gene="ivbL"
                     /locus_tag="OO369_001289"
     CDS             complement(12862..12960)
                     /gene="ivbL"
                     /locus_tag="OO369_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR5981627.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13525..13614
                     /gene="tisB"
                     /locus_tag="OO369_001290"
     CDS             13525..13614
                     /gene="tisB"
                     /locus_tag="OO369_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR5981628.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13894..15078
                     /gene="emrD"
                     /locus_tag="OO369_001291"
     CDS             13894..15078
                     /gene="emrD"
                     /locus_tag="OO369_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR5981629.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15086..15583)
                     /locus_tag="OO369_001292"
     CDS             complement(15086..15583)
                     /locus_tag="OO369_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR5981630.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15580..15942)
                     /locus_tag="OO369_001293"
     CDS             complement(15580..15942)
                     /locus_tag="OO369_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR5981631.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15932..16279)
                     /locus_tag="OO369_001294"
     CDS             complement(15932..16279)
                     /locus_tag="OO369_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR5981632.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            16388..16801
                     /locus_tag="OO369_001295"
     CDS             16388..16801
                     /locus_tag="OO369_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981633.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16848..18290)
                     /locus_tag="OO369_001296"
                     /pseudo
     CDS             complement(16848..18290)
                     /locus_tag="OO369_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18325..20040)
                     /locus_tag="OO369_001297"
     CDS             complement(18325..20040)
                     /locus_tag="OO369_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCR5981634.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFLRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20207..21100
                     /locus_tag="OO369_001298"
     CDS             20207..21100
                     /locus_tag="OO369_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5981635.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21097..21911)
                     /locus_tag="OO369_001299"
                     /pseudo
     CDS             complement(21097..21911)
                     /locus_tag="OO369_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21911..23527)
                     /locus_tag="OO369_001300"
     CDS             complement(21911..23527)
                     /locus_tag="OO369_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR5981636.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23822..24496
                     /locus_tag="OO369_001301"
     CDS             23822..24496
                     /locus_tag="OO369_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5981637.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24535..26196)
                     /locus_tag="OO369_001302"
     CDS             complement(24535..26196)
                     /locus_tag="OO369_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR5981638.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26392..26820)
                     /gene="ibpB"
                     /locus_tag="OO369_001303"
     CDS             complement(26392..26820)
                     /gene="ibpB"
                     /locus_tag="OO369_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR5981639.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26932..27345)
                     /gene="ibpA"
                     /locus_tag="OO369_001304"
     CDS             complement(26932..27345)
                     /gene="ibpA"
                     /locus_tag="OO369_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR5981640.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27339..27411)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27650..27982
                     /locus_tag="OO369_001305"
     CDS             27650..27982
                     /locus_tag="OO369_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR5981641.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27984..29222)
                     /locus_tag="OO369_001306"
     CDS             complement(27984..29222)
                     /locus_tag="OO369_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR5981642.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTNTWTPDSQWLVFDVRPSGAS
                     FTGETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFH
                     HRRGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLD
                     PALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGW
                     VGNHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVV
                     QRRLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKL
                     THNKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSP
                     DGQWLAWMEGGQLWITETDR"
     gene            29323..30423
                     /gene="cbrA"
                     /locus_tag="OO369_001307"
     CDS             29323..30423
                     /gene="cbrA"
                     /locus_tag="OO369_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR5981643.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30384..31721)
                     /locus_tag="OO369_001308"
     CDS             complement(30384..31721)
                     /locus_tag="OO369_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5981644.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31796..32944)
                     /gene="dgoD"
                     /locus_tag="OO369_001309"
                     /pseudo
     CDS             complement(31796..32944)
                     /gene="dgoD"
                     /locus_tag="OO369_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32941..33558)
                     /gene="dgoA"
                     /locus_tag="OO369_001310"
     CDS             complement(32941..33558)
                     /gene="dgoA"
                     /locus_tag="OO369_001310"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR5981645.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33542..34420)
                     /gene="dgoK"
                     /locus_tag="OO369_001311"
     CDS             complement(33542..34420)
                     /gene="dgoK"
                     /locus_tag="OO369_001311"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR5981646.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34417..35106)
                     /gene="dgoR"
                     /locus_tag="OO369_001312"
     CDS             complement(34417..35106)
                     /gene="dgoR"
                     /locus_tag="OO369_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR5981647.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35384..36040
                     /locus_tag="OO369_001313"
     CDS             35384..36040
                     /locus_tag="OO369_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981648.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36086..36898)
                     /gene="yidA"
                     /locus_tag="OO369_001314"
     CDS             complement(36086..36898)
                     /gene="yidA"
                     /locus_tag="OO369_001314"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR5981649.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(37012..37410)
                     /locus_tag="OO369_001315"
     CDS             complement(37012..37410)
                     /locus_tag="OO369_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR5981650.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37650..40064)
                     /gene="gyrB"
                     /locus_tag="OO369_001316"
     CDS             complement(37650..40064)
                     /gene="gyrB"
                     /locus_tag="OO369_001316"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR5981651.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40093..41166)
                     /gene="recF"
                     /locus_tag="OO369_001317"
     CDS             complement(40093..41166)
                     /gene="recF"
                     /locus_tag="OO369_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR5981652.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFEHDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41166..42266)
                     /gene="dnaN"
                     /locus_tag="OO369_001318"
     CDS             complement(41166..42266)
                     /gene="dnaN"
                     /locus_tag="OO369_001318"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR5981653.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42271..43674)
                     /gene="dnaA"
                     /locus_tag="OO369_001319"
     CDS             complement(42271..43674)
                     /gene="dnaA"
                     /locus_tag="OO369_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR5981654.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43971..44051
                     /locus_tag="OO369_001320"
     CDS             43971..44051
                     /locus_tag="OO369_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981655.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44281..44421
                     /gene="rpmH"
                     /locus_tag="OO369_001321"
     CDS             44281..44421
                     /gene="rpmH"
                     /locus_tag="OO369_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR5981656.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44438..44797
                     /gene="rnpA"
                     /locus_tag="OO369_001322"
     CDS             44438..44797
                     /gene="rnpA"
                     /locus_tag="OO369_001322"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR5981657.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44761..45018
                     /gene="yidD"
                     /locus_tag="OO369_001323"
     CDS             44761..45018
                     /gene="yidD"
                     /locus_tag="OO369_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR5981658.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            45021..46667
                     /gene="yidC"
                     /locus_tag="OO369_001324"
     CDS             45021..46667
                     /gene="yidC"
                     /locus_tag="OO369_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR5981659.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQSAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46773..48137
                     /gene="mnmE"
                     /locus_tag="OO369_001325"
     CDS             46773..48137
                     /gene="mnmE"
                     /locus_tag="OO369_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR5981660.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11983 a        13063 c        12221 g        11061 t
ORIGIN      
        1 agcggtaagg tcgctgcggt ttaaaaaacg aaacgctatc gataagaata aaaaggaata
       61 aaagtggaat ataaagtctg gcacttctta ctgaccacgc aagcgcgttt cgtacagcat
      121 gatgagagcg atgaaagcaa actacactta tgcttcattc gatacacatt tgtaaaaggt
      181 tgaatccatg aaaccaacga cactactgct tattttcact tttttcgcta tgccaggcat
      241 cgtatacgcc gagtcgcctt ttagctcact gcagtccgca aaagagaaaa ccacggtatt
      301 acaagattta cgtaaaattt gcacgccaca tgcgtcatta tcagatgaag cgtgggaaaa
      361 gttaatgttg tcagatgaaa acaataaaca acatatacgc gaggccatcg tggcgatgga
      421 acgtaataat cagagcaatt attgggaagc gttgggtaag gtagaatgcc cggatatgta
      481 agaacagcaa caactactga ccaacgcttc tttacctgat atcaggattt cttcattttc
      541 acctttgcag taaccaacaa tgcagtcagc aacatcaatg tgccggacaa catcaacggc
      601 gaagtcagac caatattatc cagcgcataa ccgccgattg ccgcgccaca ggtattagca
      661 agctgaataa ccgccacctg aatagacccg gctttttctg cctgatcggc cagcgagcgg
      721 gtgatccacg ttgaccagcc gacgggaacc aatgcaaaag ttagccccca gataatcgcc
      781 acgccggtag caacgatttt atcgcttccc cacaacgtca gtaccaacgc actcacagcc
      841 agtattaacg gcgcgcctgc taaggccagt tttaccgaac gtttaagaat gaacgacgaa
      901 agcgacgtac caataaagct ggcgatacca aaactcaaca gcaccagcgt taagccatcc
      961 acgccgaatc ccgccaggtt catatacacc gggcgaatat acgtgaagaa agcaaactgc
     1021 ccggcgaaag acatgaagat ggcgatcatc cctgccatca cacccggacg ttgtaataag
     1081 cggaaagtat tttgtttctg atgcgagggt tcgcctggca gtgaaggcaa tgatttgata
     1141 atccagaaaa tacacagcac gcccatcacc gccgccgcat taaagacatt gcgccaaccg
     1201 ataagctcgc ctaaaaaact gcccaacggc gcggcaatca ccagcgcaat agaaaccgcg
     1261 ccgaagatca ccgacagcgc cttcggcacc gtacgcggcg gtaccagacg catggtcagc
     1321 gacgccgaca tcgcccagaa cccgcccagc gccagcccca gacaggcacg accgattaaa
     1381 agcaaactga atgagttagc aaaggaaacc agcaagcagg agagcgtcag caaaacggca
     1441 aacaaaataa caacgtagcg gcggtcagtg gcctgaattg tctgggtaat aaacaaactg
     1501 gcaaacattg ccacaaaggc ggtcacggtc accgattgcc cggcaacccc ttccgaaatg
     1561 cctaaatcct gggccattgg cgtcaacaaa ctgacgggca aaaactcaac gataatcaga
     1621 caggcgacac aaaacgccac cgagaaaacg gctgaccagt tcggtcgggt gatggcatcc
     1681 gcgccgcggt tttcggcaat aaattcactc atggtgttac ccgtggcatg tttcagggga
     1741 aaagccgacc actttaacat tgaaatgtgt gacgaattta acgtttttgc aacttttacg
     1801 gtgttgcttg agctggagtt aagatcgaaa cggaacaggg caaagattac cggatgcggc
     1861 gtgaacgtct tatccacccg acaggtcagg cgcggtctgt aggctgataa gacgaaacgg
     1921 tttcaatgca accagtgaac gccctcggca ggctgaaaga acgcgttata gcgcatctga
     1981 aaagcattaa tatcctgcat ttcaggttcg gtttcacgca gaaaacccag cgccagcctg
     2041 acctgagcat cggtaatcgg cgcggaaagg cgggcttttt gtaaaatccg gtgccattgc
     2101 agcaggttct gttcgctatc atcgacaatg acaatttgcc agataagcaa cacctgcgcg
     2161 gcgttttgca ggtgcgactc atccgggcga tgcaaataac gtaaaaattc gtcaccggct
     2221 gactttccca tttgatcgac catcgactcc acgggtacga catcgatatt cagccgatcg
     2281 ctgagacggc gaatcgcttt tctggccccg gatgtcagca cccgaaaacc aaccacaaac
     2341 actaccgcca gcgtggcgag cattatccag atcatgagtt ctcctgcgta atcagaactc
     2401 atgatatctg gaaaccctcg ccagttacta atccaccaga ataatcttca atgcgaacag
     2461 agctgcgacc accaccacac acaggttcag atcgcgccag cgcccggtgc acactttcat
     2521 gatgcagtac gacataaagc cgagtgcaat cccttcggtg atcgagaaag taaagggcat
     2581 catcaccgtg gtaataaacg caggcaccga ttcggtaaaa tcatcccagt taacgcgcgc
     2641 caggctcgaa gtcatcagca cgccaacaaa gattaacgct ccagcggttg cgtaaggagg
     2701 aaccatcgcc accagcggtg agaagaacat aactaacagg aacataacgc cgaccacgac
     2761 agccgttagc cccgtgcgac caccgactgc cacaccagaa gtactttcaa tataggcagt
     2821 aacagacgag gtaccgataa atgcgcctgc caccgaactg acgctatcaa catacagcgc
     2881 cttattcata ttggggaatt taccgttgct atctattaag cccgctttat cagttacacc
     2941 aattaatgtt cctgatgaat caaataggtt gatcagcata aaggagaaaa tgataccggc
     3001 gagttcaagt gttaacgcgc cgctcaaatc tacttcacca atgacgccgc taatatcagg
     3061 cggaatggaa tagacgccgc taaaatgaac atcaccgaaa aataatccac agcaagacgt
     3121 caccacaata gaaaccagca ccgcggcatg aaaatgacgt gatgacaaca cggttataat
     3181 aaaaaaccct aaaataccta acaacacgcc gtgagaactt aaatcgccaa tcatcaccag
     3241 cgtgtcttta ttggcgacaa taacaccagt gttttttaat cccattaagg caataaataa
     3301 tccgattcca ctggtgatac caatacgtaa acttaatgga atgttggaga tcatccagta
     3361 ccggatacga aacagcgtga gcaaaaatag cccaattgcg ccccagaata tcgcgcccat
     3421 cccggtctgc caggagatcc ccatcgcccc cacgaccacg aacgcaaaga aggcgttcag
     3481 ccccattgcc ggagccagcg ccacgggtaa gttagcaaat atccccatcg caatactgcc
     3541 gataccggca atcaaacagg tggtaacaaa caccactttc gggtccattt gtgccgcgcc
     3601 gaggatttgc gggttcacaa aaacaatgta caccatggtt aaaaaagtgg tcatccccgc
     3661 aatcaattct gtgcggacgg tggtcccatg ctgaggtagt ttaaataatc gcgaaagcgt
     3721 ccctgattca ttactcacgt aatcggtatt gtcattattc atttttttgt ccattggagg
     3781 agatttaatc ccttgctttt gtaaaacaaa tgacaacaac ggttcagtga taattatcac
     3841 atttcaattg cacattaata gatattcttt aataatctcg cgacgtttct ttatgataaa
     3901 taataatcaa attgataaaa tcaaaatgag aaaaatatga ataattccat taaccataaa
     3961 tttcatcaca ttagccgggc tgaataccag gaattgttag ccgtttcccg tggcgacgct
     4021 gttgccgatt atattattga taatgtctct attctcgacc tgatcaatgc cggagaaatt
     4081 tccggcccaa ttgtgattaa aggacgttac attgccggtg ttggcgcaga atacgctgat
     4141 actccggctt tgcagcggat tgatgctcgc ggcgcaacgg cggtgccagg gtttattgat
     4201 gctcacctgc atattgaatc cagcatgatg acgccggtca cttttgaaac cgctaccctg
     4261 ccgcgcggcc tgacgaccgt tatttgcgac cctcatgaaa tcgtcaacgt gatgggagaa
     4321 gccggattcg cctggtttgc ccgctgtgcc gaacaggcaa ggcaaaacca gtacttacag
     4381 gtcagctctt gcgtgcccgc tctggaaggc tgcgatgtta acggtgccag ttttaccctt
     4441 gaacagatgc tcgcctggcg ggaccatccg caggttaccg gccttgcaga aatgatggac
     4501 taccctggcg taattaacgg gcagaatgcg ctgctcgata aactcgatgc atttcgctac
     4561 ctgacgctgg acggtcactg tccgggtttg ggtggcaaag aacttaacgc ctatattgct
     4621 gcgggtattg aaaactgcca cgaaagttat cagctggaag aaggacgccg gaaattacaa
     4681 ctcggcatgt cgttgatgat ccgcgaaggg tccgctgcca gcaatctcaa cgcactggca
     4741 ccgttgatca acgaatttaa cagcccgcaa tgcatgctct gtaccgatga ccgtaacccg
     4801 tgggagatcg cccatgaagg gcatatcgat gccttaattc gccgcctgat cgaacaacac
     4861 aatgtgccgc tgcatgtggc atatcgcgtc gccagctggt cgacggcgcg ccactttggt
     4921 ctgaatcacc tcggcttact ggcacccggc aagcaggccg atatcgtcct gttgagcgat
     4981 gcgcgtaagg tcacggtgca gcaggtactg gtgaaaggcg aaccgattga tgcgcaaacc
     5041 ttacaggcgg aagagtcggc gagactggcg ctgtctgccc cgccatatgg caataccatt
     5101 gcccgccagc cagtttccgc cagcgacttt gccctgcaat tcacgcccgg aaaacgctat
     5161 cgggtcattg acgtcatcca taacgaattg attacgcact cccgctccag cgtctacagc
     5221 gaaaatggtt ttgatcgcga tgatgtgtgc tttattgccg tacttgagcg ttacgggcaa
     5281 cggctggctc cggcctgtgg tttgctcggc ggctttggcc tgaatgaagg cgcgctggct
     5341 gcgacagtca gccatgacag ccataatatt gtggtgatcg gtcgcagtgc cgaagagatg
     5401 gcgctggcgg tcaatcaggt gattcaggat ggcggcgggc tgtgcgtggt acgtaacggc
     5461 caagtacaaa gtcatctgcc gttacccatt gccgggctga tgagcaccga cacggcgcag
     5521 tcgctggcgg aacaaattga cgccttgaaa gccgccgccc gtgaatgcgg tccgttaccc
     5581 gatgagccgt ttattcagat ggcgtttctt tctctgccag tgatccccgc gctaaaacta
     5641 accagtcagg ggctatttga tggcgagaag tttgccttca ctacgctgga agtcacggaa
     5701 taataaaaaa agcccggcgt catgccgggc aaaagtcacc agttacgttt atgccactgt
     5761 caactgctga atttttttct cgcggcggat tttgcgttct tccattaccg ccactatcgc
     5821 catcaggcag atacaaccaa tcgcggcgat atccagcgcg gcgaaggtgc ctgcccagcc
     5881 ggtaaggccg aataccggcg tcccatcggc aatcattccc agacctaact tggcaaagct
     5941 gtcaccaatc aggtaagcaa aggtgccttt aataccatcg gcagcgccaa tcgctttttt
     6001 aggtacaaag ccaacagcag ccacaccaat caacaattgc gggccaaaga ccaggaaacc
     6061 caacgcaaag agagaagcca gatagatata ttcgttactg gcatgttgat acacaccgag
     6121 cgtggcgata atcagcgcca gcgcgatgca ggccaccagg ccacggcgac cgttcgccag
     6181 gtcagagagc cagccccaca gcagcgtacc gaccagcgca ccagcttcaa acagcgtaaa
     6241 gccctgaatc gccaccgctt tagagagttt cagttcctgg aacgcgtata cggttgacca
     6301 ctggtcgata ccaatacgta ccacatagag gaaaatgttg gcgaagcaca gcagccagat
     6361 cactttgttt ttcagcacat actcaacaaa gatctgccac ttggtcatat cggtagattc
     6421 tgtctctttg tcctcttcgc tgatctcctc gccgaacagt tcttcagctt tgccgaggcc
     6481 ataagattcc ggggagtcgc tgccgtaacg caggccgata aaaccgacaa tcagcgcgat
     6541 aatcgacggg aagataaaca tgccgatgac atggccatcg aacaggtaat ttgccccgaa
     6601 cagcgccaca cctgctgcgc ctgcaccgcc aaggttgtga gaaatattcc agaaaccgag
     6661 gaatgtcccg cgtttacgac gcggcgtcca tttggtgatg gtggagtaac tgcacgaacc
     6721 gccggtactc tggaaaaagc cgcttaaggc gtagaaggca atcatcagga acaggctaac
     6781 cgagccgctg cccatactgg cactgaagcc cagcatacaa atggcagaga ggataagcat
     6841 gaacggcagg aattgtttgg tgtttttgcc gtcggcgtag tatgaaacca acgttttacc
     6901 cacgccataa gtgatggaga aacccaggcc gatcatcccc agctgcgtca tgctcaaccc
     6961 gtaggtcgaa atcatatcgt tctgcgcgat gttaaagttc ttgcgaatca ggtacatcgt
     7021 caggtagccg ataaagacca ccaggtagga ttgcatgaac ggtttgaacc acattttgcg
     7081 ccgcacttcg agcggaaggt ccagggtcgg cttgcgaact tggtttagga aagccagcat
     7141 gggttactcc tgaaatgaat acctgcgtga ggcatgcatt gtaaaaatca gcagcaaatc
     7201 aggcctgaga tagcgtccaa gaaaaacctg gaaaatttct tagttttgcc ggatatgaag
     7261 tgaaaaggtg agatgcatca cgcttcacgc ggtgtctggg cgttcaaaaa gggcagtaac
     7321 agcagtgcgg aaatcccggc ggcgatagag ataaccacaa aaaatccgct ccagtgccag
     7381 gtatcgagca ctttcgccag cggccaacca gcaagcgacg cccccagata agcaaacaag
     7441 ccgacaaacc ccgtcgccgc ccctgccgcc tctttgtggg aacactctgc cgccgccata
     7501 ccaattaaca tctgcgggcc aaagacaaaa aaaccaatgg tgaagaagca ggttgcctgc
     7561 atcacgtagc tggcaaatgg catcagccac agcgagccga ctgaaagcaa aattccggcg
     7621 gcgaaaatca aattcatcgg cccacggttg ccgttaaaca atttgtccga gccccaaccg
     7681 gctaccagcg caccgataaa tccgcccagt tcaaacatcg tcactgccgt attcgccgtg
     7741 accagatcga cgcccagcgt ctcggacata tacaaattgc cccagtcgtt gatcgccgcc
     7801 cggaccacat agaccagcac atagcaaaac gaaagcagcc agatatacgg attcagcaac
     7861 acatatttgg tgaggatctc tttacgcgtc aaccctgccc cttcttgttg ttgagcaatt
     7921 tccagcgcgt cgtgccgcca ttcaccgacc gccggtaaac ctaacgcctg cgggcgatcg
     7981 cgtagccgcc agcagagaaa aatccccacg actatcgcca tacaaccagc aatcatcatc
     8041 ccggcacgcc agccgtaatg cagcgcgctc gctgccatca caatgggaat gagtgcgccg
     8101 ccgacgttat gcgccgtgtt ccataatgcc caccaaccgc cgcgctcggt acgtgaatac
     8161 caggccgtta acagacgcgc acacaccggt gaaccccagc cctggaaaaa ggcgttcagc
     8221 acccagagca cggcaaacgc ccatagcgac gtcgagaagc caaacagaat gttcatgatg
     8281 cccgtggcga taagccctat ccccataaaa taacgggcat ttgagcgatc gctgacaatg
     8341 ccggagacaa acttcgacac gccgtaagta atgtaaaaca gggtcgctaa caggccgata
     8401 tcgctacggc tgagcacgcc gttagcaagg atttctggta ccgcggcgtt aaaacttttc
     8461 cgcgtgaagt aaaacagcgc gtaacccagc cagatggtca gcagaatatg ccgacgccag
     8521 tagcgataac gggcatcaat ttcatgttta tcagtcatta atggcgcatc ggcaggcgct
     8581 ttcagaaacg gcaacatcat cgaaccttag acataacgtt gaggtagaga aacgctgaca
     8641 cgcgtgccgt gcagacagga aatgtgtaat gtgccgccca gcgccgttac gcgctcgcgc
     8701 attccggtga ggccaaaacc ttgttgcccg gaacccggcg gcaaaccgct gccatcgtct
     8761 tcaatcacca gcatcaaccg ttcatcctgc tgccagcctt gcagcgtaac cgcgctggca
     8821 tcggcatgtt tcacaatgtt gttcagccct tcctggcaga cacgaaacag cgtcacgcgc
     8881 tggttttcgc ttaatgctga ttcatcgata cgccattcga gatggctgac aatgccgcgc
     8941 ccttccagct ccatttcccg catcagtgag cggatggcct gctccagggt gagatcatcc
     9001 agctggcgcg gacgtaaccg tcctaacaaa cggcgcaccg cgtcgtaaac gcccagcgac
     9061 agttgttcga tgagctgccc gctctgcttc acactggcgt tatctgccgc cagccgctga
     9121 acaatgcccg cctgagtacg aatagcagtg atggtctgac cgatatcgtc gtgcagctca
     9181 cgcgccacgt cgcggcgcac gctctcttcg gtttctaaca aacgttcagc cagatgctga
     9241 ttgcgcgcca gttccttttg tagcgactgg ttaagttcac gcaaccgctg gatgcctgcg
     9301 cccagtaaca accctgtcag actttgcacc agcaacgaga gtaataaatc caccagatga
     9361 tcgcgccagg tttgactggc gatcagcgcg atggcgttca tcaacgtcgc aatcagcgct
     9421 ccttgccagc catagtgcca ggccagcgcg ataatcggca gcgccagaca gaatggcgta
     9481 aagcgcgaca gttcgtccgg caatcccaac tggagccaga gactgataac aaacagcagc
     9541 aagtaccaga ccagatgccg cccgcgccag ttgatgggct gagaaaccag tgacgggccg
     9601 agcggcagcc aggtgttatt ggcgagatag tgccagaaca ccagacatat cggggccagc
     9661 gtcagaccgc cagttaaagt cagcaacagc gcattccacg actctttgcc gtgccaaagc
     9721 cagggcagcg actgcaacaa cgccgccgcc gttaacgccg caccctgtaa cagcaaggtg
     9781 cgccagtcac gctgatggcg atagcgcgag atcagcgcta cgggcagtaa cgtcagcaaa
     9841 ctaccgatca tcaataacgg aaaatgggtt aaaccgaccg cctgcgttag ccagtaaatc
     9901 agcagccact ccgcacccag caatacgggc cagtatccgc gcgggcattg cagcattagc
     9961 cccagacgca gaccaaacgg aaataacagc accgccatat cagggcgctc aaccagatgc
    10021 aggctgatac tccataggca aaaccatgcg gcagagaaga taaaaaagca ggcaataacg
    10081 gtaattaagc gggagaacaa cgtcttcatc accagccatc aaacatgcgg cgcgccagct
    10141 ctacgtcgtt actgacgccc agtttttcca tcagattggc gcgatggacg tgtaccgttt
    10201 tcggtgacaa gcccagttcg gcggcaatct ctttcaccgc cattccttgc gccagttttt
    10261 ccgccacctg gcgttcacgt ttggtcagcg ggtcctgacg accggatgcc agtttaatgg
    10321 caatatccgg cgtcagataa cagccgcccg tggcaaccgt atgcaccgca gcaatgagtt
    10381 catccgggct acagcgtttg gaaagaaagc cgcgtgcccc cgcgttaagc gcctgctcaa
    10441 ccagcgcagg actgtcgtga acggagagca taatcgtcgc catacctttc ggcagctggc
    10501 ttagcagctc cagaccggag atatcgggca tggagatatc gcaaatacac acctgcacac
    10561 cgcgccccgg cagccccgcc agcgcctcgc gccccgaacc aaactcggca actacctgca
    10621 aatcaggttc cagccccagc agctgcgcaa agccggagcg gacgatgagg tgatcgtcta
    10681 taagggcaac ggtgatcatg gtcttgtcct ggcgggtaaa aaaaaacgcg cttaccttaa
    10741 cgataagcgc gatgttgttc aagccttgag cggttactga aaaaacaccg cgatcttgtt
    10801 aaacatcgtc ggatcggact gattacgctg cactttcacg acatcttcca gcttagcgat
    10861 ttggcttatc atctgctcca gacgctggtc gtcattgacc agtagccaga tatggctttt
    10921 gtcgctgtcc tgaatcggca gacaaagaat gccttcaacg ttaaaagcgc ggcgggcaaa
    10981 aaggccacaa acgtgggtca ttacgcccgg atggttgcga acggtgagct ccagaattac
    11041 gttgttatga gttgtgtttt gcatggctta ttcccccacc atttcagtat tcgccgcacc
    11101 tggcggcacc atcgggtaaa ctttttcttc ggcatcaatg cgcacatgga tcagcgccgg
    11161 gccagggcga ttgatgattt cctgcaatgc agcctgcgga tcggcttcgt tattcaaatc
    11221 acaggtttcg aggccgaatc cggcggcaat ctgcataaag ttgattttgc ccggataggt
    11281 ggcggcaaaa acgccttgct cgtagaacag actctgttgc tgatgcacca gccccagcgc
    11341 ttcgttgttc atcagaatga ttttgacatc cagctgattt tcactggcgg tcgccatctc
    11401 ctgaatattc atcatcaggc tgccgtcgcc ggagaaacac aacactttgc gatccgggtt
    11461 cgccagcgcc gcgccaatcg ccgcaggcag gccaaaaccc atcgtgccca gcccaccgga
    11521 ggtcagccac tggcgtgggc gattgagcgg ataagcttgc gcggtccaca tctgatgctg
    11581 acccacatcg gtggtgataa tcgcattgtc atcgacacag gcggcaacgg cgttgatcag
    11641 gccgtaatgg cttaatggat cgcacgcttt cgggattgga cacgggaatt cgcgctgcaa
    11701 atccgctacc aactggtgcc actctgcacg cggttgcgct tccacccgcg ggatcaattg
    11761 cgccagcacg tcatcaacat ccgcctgaat cgccacatgc ggctgcttga ttttacccag
    11821 ctctgcgcga tcgatatcga catgaataat tttggcattc ggacagaact gctcggtttt
    11881 gccaatcgcc cggtcatcaa aacgcgcacc gagcacaatc aacaaatccg cctcctgcaa
    11941 aatatagttg gtactgcgca cgccgtgcat ccccagcata cccagcgaca acggatgcgc
    12001 ttttggcagc atgcccagcg ccattaaagt catggtggta ggcagttgcg ctttctccgc
    12061 cagttcacgc acccgcgcag gcgcattgat cacaccacca cccagataaa gcaccgggcg
    12121 tttggcagcg ttaatcattg tagctgcgtc acgaatgctt tcttcgctaa aggcgggtgc
    12181 aacggctttt tctgccaccg cgggctgagc ttcaatctca aaaaccgccg tttgtacatc
    12241 cttaggaatg tctatccaca ccgggctagg gcggcctgat tgcgcaatgc ggaaggcatc
    12301 gctcatgacc tgtgggagct cttcgatatg tctgaccaga tagttgtgtt tggtgatggg
    12361 gatagagatg ccgtaggtgt ccacttcttg gaaggcgtcg gtgccgatca tcgaggcggg
    12421 aacctgacca gtgatgcaaa tcagcgggat ggagtccagc cgcgcatcag caatggcggt
    12481 caccaggtta gtcgcacccg gcccgctaca ggccatacag accgccggtt taccgtcggt
    12541 gcgcgccatt ccctgagcga taaaccccgc gccctgttca tggcgagcca gaatatggcg
    12601 gatttgcgtg ctttggctta aggcgtcgta aacaggcagg atagaaccgc ccggaatgcc
    12661 cgtcacaatc ttaatgccct gctgttccag gaaatgaacg ataaattctg cgccggtaaa
    12721 gcgcttacgc gtcgatgttg tgcccgaact tgccatgctc cagtcctttt cttctgggcc
    12781 gactttccgg gaggtgctta aacgaaaaac cccgcccggt ttgcgccggc ggggttttgg
    12841 aatcgtgtgt tgttccagtc cctacggcgc attgccgacg accaccacca cacgcacgac
    12901 gaccactgcg gcggatggcg cagttggtag tagttttgcg ttgaacatgg aagtagtcat
    12961 tggggacctt gttggttttg tgtttaacaa tatttataca agcacagctt tacaggggag
    13021 acaatggaaa atttttcagc aagggaaaat tgaggggttg atcacgtttt gtactgaatt
    13081 gcagataaca aaaaaccccg ccggagcgag gtttcgtcag ccgcctgcgg ctggtaaccg
    13141 caaagcacac tgtattatgt caacactgaa agtatacgtg ttccgcgcag aacgcgcaat
    13201 ttcggcacga attttgacgt atttagtgca tagttgagta tcgttcacag tttgcgtttt
    13261 gtccaaatat tactgtttat ttatacagta agcttctata atatcactgt acgcaatgtg
    13321 ttatgcgggg gccgcatcgt tacccggcgc actaagtcct ggctgaaacg ggtggtgccg
    13381 tcagcgcctt aaccccgcgt gagcacactg tgttatgtca acaagcacaa cgtttctcct
    13441 tgagataccg cgtgcacaac agctggcaac aggcagcgga aaggtacgtc agctggcagt
    13501 gctcctgaac cacaggagac gcgtatgagc ctggtggata tcgccattct tatcctcaaa
    13561 ctcattgttg cagcactgca actgcttgat gctgttctga aatacctgaa gtaattcaga
    13621 ttcaagtcgc accaaagggg agcgggtaac cgctcccctt ttatatttag cgtgcgggtt
    13681 ggagtcggat gcgatgctga cgcatcttat ccgccctacc atccctcccg gcaacattta
    13741 ttgccgcttt tgtttacata ttctgccgct aaacaattcc ccattcctgg cgtatatctg
    13801 gctaacattc atcaatgtga tagattcctc tcccgcattt atgggaatgc gtagtgactt
    13861 attctaatta tttttataaa agcatccgtg ataatgaaaa ggcaaagaaa cgtcaatttg
    13921 ttattgatgt tggtattact cgtggccgtc ggtcagatgg cgcaaaccat ttatattcca
    13981 gctattgccg atatggcgcg cgatctcaac gtccgtgaag gggcggtgca gagcgtaatg
    14041 ggcgcttatc tgctgactta cggtgtctca cagctgtttt atggcccgat atccgaccgt
    14101 gtcggtcgcc gaccagtgat cctcgtcgga atgtccattt ttatgctggc aacgctggtc
    14161 gcggtcacga cctccagttt gacggtattg attgccgcca gcgcgatgca ggggatgggc
    14221 accggcgttg gcggcgtaat ggcgcgtact ttgccgcgtg atttatatga acggacacag
    14281 ttgcgccatg ctaacagcct gttaaacatg gggattctcg tcagtccgtt gctcgcaccg
    14341 ctaatcggcg gtctgctgga tacgatgtgg aactggcgcg cctgttatct ctttttgttg
    14401 gttctttgtg ctggtgtgac cttcagtatg gcccgctgga tgccggaaac gcgtccggtc
    14461 gatgcaccgc gcacgcgcct gcttaccagt tataaaacgc ttttcggtaa cagcggtttt
    14521 aactgttatt tgctgatgct gattggcggt ctggccggga ttgctgcctt tgaagcctgc
    14581 tccggcgtgc tgatgggcgc ggtgttaggg ctgagcagta tgacggtcag tattttgttt
    14641 attctgccga ttccggcggc attttttggg gcatggtttg ccgggcgtcc taataaacgc
    14701 ttctcaacgt tgatgtggca gtcggttatc tgctgcctgc tggctggctt gctgatgtgg
    14761 atccccgact ggtttggcgt gatgaatgtc tggacgctgc tcgttcccgc cgcgctgttc
    14821 tttttcggtg ccgggatgct gtttccgctg gcgaccagcg gcgcgatgga gccgttcccc
    14881 ttcctggcgg gcacggctgg tgcgctggtc ggcggtctgc aaaacattgg ttccggcgtg
    14941 ctggcgtcgc tctctgcgat gttgccgcaa accggtcagg gcagcctggg gttgttgatg
    15001 accttaatgg gattgttgat cgtgctgtgc tggctgccgc tggcgacgcg gatgtcgcat
    15061 caggggcagc ccgtttaagc gcacgtcacc gcagcatcgt catcagctcc atgggagaac
    15121 gatgctgctt tatcagatca cgcatcaccc gcatatgcgg tgcggagtaa gaataaaacg
    15181 cctgatagcc cgcacaaagc acgctgtctg gcgtctcctg ccgagcgtgc gggcacaggc
    15241 gatgccagtc gcacgcctgg cagtcacggc gcggttgcgg gttttcgctg ccacaccatt
    15301 tttgtaaggt ggcgtcaaaa agctcaatcg agatacgact gatatctgcc cgcacccaac
    15361 actcaaacag ggcaattaaa aatttccccc acagctctgc tgtcaccgaa gcggccgtca
    15421 gatcgccccg gtcatcaaat tcaagatagg gaatgagctg tacggagcgg atgccccgct
    15481 gtttgaaatt gtgataaaaa tcggcaggtt gcagagcatc cttgtactct acaaccagct
    15541 tatgcctgtc ctcttcagca tcaatgacct gacttcctgt catgcgactc tctcaataaa
    15601 tacgataagt tgatgtatat gcgttacagc aaacagtatt gcgagagata acaccgcgag
    15661 ggagatcaaa aatttgtcac ggactacgtg aaattgggaa aaatcgctat tcgtgacatc
    15721 cattaaattg cgattacggg tgtagtgcca gaggatcagc gccacgatgg cgaggatgcc
    15781 aatcgaaatc cagaataaca tgcccgcctg gtgccagttg tgtttgatag ccaacgccat
    15841 caacgcgccg tagcccagca tggtacgaaa ccacgccagt gatgtgcgct ccggctgcag
    15901 ccctgggtcg gcaatgcgtc tggctttacg gctatccggc atacaacacc agtcccataa
    15961 cgatcaccgc cacaaccatc aatattaagc tgatgattaa taagctgttg gtgtagggca
    16021 ggtcctcttt cagacgcatc gctttttcat tgcgtaacca gcgcaggtag ccatacattg
    16081 ctaacccgcc ggaaaacaga cacaacaaca acgccagcag ttcacgaatg acgggcgtgg
    16141 cgaaatctgg cgcaagttga tcaagcccaa cccccgccgc cagaaatcct agtgcggtac
    16201 ggatccacgc cagaaaagta cgctcattag ccagcgagaa gcggtaatcg ggcgcttctc
    16261 caaggcggga aattttcatt gttgctcctt tgcaatttca ctaacgctct atctttagcg
    16321 gtttttttta agaatttcgt ccgcccgttt cctgatatgc ttttgccatc cgctacaagg
    16381 aggtcagatg ggtatcatag cgcaaaataa aatcagttca ttgggcatgc tgttcggtgc
    16441 catcgcttta atgatgggga ttatccactt ttcattcggc cctttttccg caccaccgcc
    16501 aacgcttgaa agcatcgtgg cggacaaaac ggctgaaatt aaaagaggtc ttctcgccgg
    16561 aataaaaggc gaaaaaatta ccactgtgga gaaaaaagag gacatggata tcgataaaat
    16621 cctcgatcaa agcggtatcg cactggccat tgctgcgcta ctttgcgcat ttattggcgg
    16681 tatgcgtaag gaaaatcgct ggggcattcg cggcgcgctg gtttttggta tcggcatcgt
    16741 gtgcagcatt ttgctgatat tcctgatttt ttccttcctg accggcggtt cgctggttta
    16801 agcagcctga ataatgcccg ccgggtgatc atcagcgggc attatcgtca gaatttctgt
    16861 tttttctcct ccaccttcac tccttgcgtc ggtaggccgg tgtcatagtc gcgtaccact
    16921 ggcgaatagc catcttgtgg gcgtgggcga aacgccccca tccagcgcgg tcgtgcatct
    16981 ttacgccacg gacgcagact ccattggtaa ctgcggaacg gatcgcgaat tttgtccatg
    17041 taatccaata aggcgtcatg cattttgctg cgaacgtctg caaaacggat atcatcgatc
    17101 aggttatgca tttcatttgg gtcattacgg cgatcgtaaa gttcatcact ggtgaagagg
    17161 ttgagcacca gtttaaagtc atccgtcacc cagcaacgca ccggaataaa accgccaaag
    17221 ctgtcatgct caatctcgta gcggttaaat tccaccatca cgccgcgtgg ctctttcacg
    17281 gcaaggatat tttcccccgg cagaatctct ggtttttcaa tatctgccag cgccatcatt
    17341 gtcggcagta aatcaatatg actgactggc gtatcgacct gtcgccgctc cccttgcggc
    17401 gaacggatga tcagcggaat acgggtgatg tcgtcataca tcgccgctcc tttactgatc
    17461 agcttatgtg cgcccatcat ttcaccgtga tcggaggtat aaatgaccca cgtattttca
    17521 cgttgctctg gcgttaaggc attgatgaca cgtccgattt ggtcatcaac aaagtcattg
    17581 caggcaaaat agagcggatg gtgataaagc ccgtcatcac cgactggcga tggcatcgcc
    17641 tgcgcccata agcggtgatg ttccggtttg ttcgccaggt catcctgtga tttctcgcca
    17701 agttcgtagt aaaaatcagc gtatttctct aaatactcca ccggacaggt gaacgggtga
    17761 tgcggctcat catacgaaac caccatcagg aagggttcct cggcgcgcac gggctattgc
    17821 agaaaatcta ccgctcgatt gctgatgcgg tgcgcccagg tgaaggtttc gtcaatatgg
    17881 ttcgcctgta aatcttcgac gctgtttagg ccattgcgcc acaggctgat ctctttttcc
    17941 gtcagttcgc taagatagtt cgccccatca aaccagtaat cagcatccca ctccggcgga
    18001 cactcgccag tgccgaaata gtcatgaccg tcgagatgcc atttgccgat gtaacaggtg
    18061 tgatagccgg catccttaaa gtagcgcccc atagtggaga tgtttttgcc tggcgcgacg
    18121 ttgttggtcc acgggccgga ctggttagcg tagataccgg tgaatagtcc ggcccgtgca
    18181 ggcgtacaaa ccggtgaaca agtgtaggcg gaattaaagc gaataccttc cgccgccaga
    18241 ctatcaatat tttgcgtatt cagcggttta ccgctatagc aaccgaccat attggtggcc
    18301 tgggtatcgg tcaattgggg cgtttcatag gctatccttc gcgtcgggag taaaggttac
    18361 ggctgggtcg tgacgatgcc accagctgtc aaaaatcagg tagacaatca ctacggcagc
    18421 gatgaaataa ctgatcatcg ctaaccagcg cgtaccgtag ccgccgaatt cagccagccc
    18481 gacatagacg ccaatcatcg cgaacaggat accaattgac gcgatcttga cgtttttcca
    18541 cggtttcatg tccaccgcaa acgcatcttt gaaggtgaac ggcgtggcgc gcggtttgat
    18601 aaaaccgatc accagcatca cgaccacgtt gatgcagaac gtacaggcca gcacatagag
    18661 gaaatggaag tcgaacttca ccagatagtt gatggtgatg tagctgatta tgccaatccc
    18721 catcgccact tttgccgcca gcgccgggat gcgcagaaag aaaaagccca taatgatgat
    18781 ggtaaccagc ggcacgttgt aaatgccgtt gagctgtttc atccagctat acagcccctg
    18841 cggcgcgttc gcgatccacg gcgctaccaa aaccgaaacg atagcgataa agaaaccaaa
    18901 tttgcgcccg acggtgacca gctgctgcgg ctcggcattc tggttaatga tgcgacggta
    18961 aatacccata ctgaataatg tactggcgct attcagaaag ccgttgaagg tactgatcac
    19021 cgcaccaaat aacaccgcgc cgaagaaacc ctccagtggc actggcagaa cgttattgac
    19081 cagcgtcggg taggccatgt cggctttcgg tagatcctga tacagatgaa acgcaatcaa
    19141 ccctggcagc accagtacca gcgggtcgag cattttcagc accgccgtta acagcgcccc
    19201 cttttgccct tccgccaggc tttttgacgc cagcgtgcgt tgcacgatgc cctgatttgt
    19261 acaccagtaa aaggtgttca ccagaattaa accggtaaat gccgcgccaa tcggcaaggg
    19321 atcggtcggg ccaccgactg agtttaattt ctcggcgtga acggtggtaa tttgctcaat
    19381 gccctgcata aagctgccct tgcctatcgc gatcaggcca aataccggca ccatcaaccc
    19441 gccgataacc agcccaatac cgttgatgga gtccgccact gccattgcgc gcagtccgcc
    19501 gatcaccgca tacaaaattc ccgccagacc aagcaaaatt accagtagcc agatagccgc
    19561 accgtgagaa atctgtagcg attccccgac gtgaaacagg ctgttgagcg ccaacgcgcc
    19621 ggagtagaga acaatcggca gaaagcagac gccggtggca atgaggaagc agaagtcgat
    19681 gataatacgc gtcgttttat cataacgttc ctccagaaaa tcggggatgg tggcaatgcc
    19741 gcgcttcaga tagcgcggta aaaagattag cgcgaggaag atcagcgtca ccgctgaagt
    19801 cacttcccag cccatcaccg acatgccgct tttgtaggcc tgcccggaaa gaccaaccag
    19861 ttgttccgtg gaaaggttgg ttaacattaa cgaagcggca ataaccggcg cttttagtga
    19921 acgtccggca agaaaatagc cttgttgcga tccggtatct gttttgcgaa ccttccacca
    19981 ggtgatcacc gccaccagca gcgtaaaacc gacaaaactc aagatttgta acgaattcat
    20041 ctcatagccc ttatttatgt ttatgtcccg gacgcatgaa tatagggaga ggtaaaatgc
    20101 gtcgggtcag gcagtcagat tcttaaaata caaacgtcgt atccctgaac ggattgtttt
    20161 ctgttaagtt caggttgtga gcataattcg ccggagggat attaaaatga atggaaaatt
    20221 gcaaagttca gatgtaaaaa acgaaactcc gtacaatatt cccttattga ttaacgaaaa
    20281 tgtgatctcc agcggaattt ctctgatctc gctctggcat acctacgccg acgagcatta
    20341 ccgggtgatc tggccgcggg acaagaagaa accgcttatc gccaactcat gggttgcggt
    20401 gtataccgta caaggatgtg ggaaaattct tttaaagaat ggcgaacaaa taactctgca
    20461 tagcaactgt attatatttt taaagccaat ggatattcac tcttatcact gtgaaggttt
    20521 agtctgggaa cagtactgga tggaatttac ccccaccagt atgatggata tttccgttgg
    20581 tcagcaaagc gttatttata atggcgaaat ttataatcag gaactcaccg aagttgctga
    20641 gttaataact tcaccagaag caataaaaaa taatctggca gtcgcttttc tgacgaaaat
    20701 tatttatcag tggatttgtc ttatgtccgc agatggtaaa aaagatccac aacggcggca
    20761 aattgaaaaa ttaattgcca ctttacatgc cagtctgcaa caacgctgga gcgtagctga
    20821 tatggctgcc acgatcccct gtagcgaagc ctggttgcgt cgtctgtttt tacgctatac
    20881 cggcaagacg ccgaaagaat attacctcga tgcgcgtctg gatctggcgc tatcgctatt
    20941 aaagcaacaa ggaaactcgg ttggcgaagt cgctgatacg ctcaacttct tcgactcctt
    21001 tcatttcagc aaagccttta aacataaatt tggttatgcg ccgtcagccg tgctaaagaa
    21061 tacggaccag cacccaacgg atgccagtcc acacaattaa cgtaactctg gccagtacgc
    21121 tttgttggcc tccagcaact catccagaat agctttggcg accgaggcgc tcggtaccgt
    21181 tttcgacaac gtaatcgcct gccacaggtg ctgatatgaa cgctgttccc aggcgtccac
    21241 gaccagtttt tccaccgcca cttgctgact cattaaccct ttctgaaact gcgggatatc
    21301 gccgaccacc agtggttcag gccgtagtag cgcacgcgca tctgtttgcg atcttgcagc
    21361 ccaacaattt gcgccatccg gctttcaata ccgattggca tgtcacagat gttgaggatt
    21421 ttcgcattcg ggcgcagacg acgcgtggct tctgcgacaa ttgctgccgg gttggagtag
    21481 ttgagcatct aggcgtttgg tgaatatttt tccatataat ccaccagttc caggacgccg
    21541 ccaatggaac gcatgccgta cgctattccg ccgggtccgc aagtttcctg accaacaaca
    21601 ccgtggcgca gtgggatttt ttcatccagt tcgcgcatcg ggtacttgcc gacgcggatg
    21661 tgcgccatga caaaatcaac gtcgctgaac gccacttcag gatcggtggt gtaactaaac
    21721 gcaatgtccg gcgctttttc tttaaggatg actttacagg cttcggcaat cacttcctgc
    21781 cgcgcgccat cgttatcata aaatttcagt gcacgaagcg ggaaacggtc ctgattcgcc
    21841 aggagcatca acacgatgcc tggcgtaaag gtgcttccac cgcctgcgac aaccactgag
    21901 aatttggtca ttatactgcc tccgtaatgg caacattttc tgctgattga tgagaattaa
    21961 ttaagctatc gagctgttca cgcaattggg atacatgcag accaatgatc acctgaatgg
    22021 catcgccact acggaagacg ccgtgcgctc ccagcttttt aaagacttcg tcatccagcg
    22081 tttgtgacat gtcatgcagt gcaatacgta gacgcgtcgc gcaattgtta atgctggaga
    22141 tattgccgac cccgcccagg gcttgcagga taccggcagc ctgatccagc tctttttttg
    22201 gctctgcagc ggtggtttgg cctcgcgagg ctttgtattc ggcttttgag tagagtttca
    22261 cttccgcatc ttcacgtccc ggcgtgcaca tgttgaactg cagaatcagg gtgcggaaaa
    22321 ccacgaagta cagcagggta aagcacaacc caatggcgat ttgggtcagc atcatatccg
    22381 cgtggttgct gaacatcggg atccagtttt gcggtaaaac ctggtcaatc agacctccgc
    22441 ccatgttgcc caccacacca aagagataca ttacggtcga cattgaggcc gccagcacgg
    22501 cgtgtaccgc aaacagcaac ggtgaaatga acaggaaggt aaattccagc ggttcggtaa
    22561 ttcccaccag catggcggtt aaggttgcgg gaatcagcaa gcccgccact tttacccgat
    22621 tttccggtgc ggcagtgaag tacatcgcta aagaaatgcc cacggcacca aagatttttg
    22681 agttaccgtg cagggcaaaa ccgccttccg ggaacaacga tttcagtggc tcggcgctca
    22741 gactgaactc ttgcagatgc tgcgcccagt acatctgaat gccgccttca acagctgccg
    22801 gaccaaagat aaacggtccg tagatgaagt ggtgtaaacc ggttgggatc agaatacgtt
    22861 cgaggaaggt gtaaacccac accccaagcg cacccgccga acgcaggaac gcttgcagag
    22921 attcaatccc catttgtact tttggccagc cgagcaatgt cagccaggca caggggatca
    22981 tcaccaggaa ggcgataatc accacataag acgtcccctg gaaaatgccg agaaaaaccg
    23041 gcagtttttt atcgaacaga cggttatgca gcgccgtcac aatgccggaa atgataattg
    23101 cgccgataat gctggtatcg agggttttaa tcccggccat cattgtcaga ccgctacctg
    23161 ccaccgcgtc ctgagtgaaa tcgacgccga agtagcttcc ccaggtcatt cccatcgcat
    23221 tgatgaaata gttccaggtc aggaaactca ccatcaccgc cagacaagca cgtccctgcg
    23281 cttgcttagc aaggccaatg ggtaaaccga cagcaaaaat cagcggcata ttacggaata
    23341 ccgtccaacc gccctcttca ataatgtgta cgatttgcgc gaataaactg ttcggatcgg
    23401 tcagtgattc cccgacaaac atcgggtttt gcagcaagat ggcaagaccc accacaatcc
    23461 cggcgaaggg aaacagcagc actggcgtga acatcgcgcc gccaaagcgt tgaatttgac
    23521 tgagcatttc cacatcctct tttctcaatt ctgtaagtag gaagcctgat tggttatgcg
    23581 gcagacctgg agcgaggata ggaagatgcg atcggcagaa catgacgacc caatgggatt
    23641 cgtgatcgct ttcatgcttt tcgttgggcg gtagagatct acgttttcgc gaaaaagtag
    23701 acatctgtga gcacaggtac acagcatcga acacatcttt aaaaaaaaga tttttttcaa
    23761 tcgattaagc agaacttgtg ggcgcattac ccgtgcttgc aggcaaaaaa gagagctaga
    23821 gatgatctac aaaagcattg cggagcggtt aagaattcga cttaattccg cggatttcac
    23881 gctaaacagc cttcttcccg gtgaaaaaaa gctggcggaa gagtttgcgg tatcgcggat
    23941 gactatccgt aaagccattg acctgctggt agcgtggggg ctggtggtcc gccgccacgg
    24001 cagcggcact tacctggtgc gcaaagatgt gctgcatcaa accgccagcc tgaccggact
    24061 ggtggaggtg ttaaaacggc agggaaaaac ggtcaccagc caggtgctga tttttgaaat
    24121 catgcctgcg cctccggcca ttgccagcca gttacggatt caaatcaacg agcagatcta
    24181 cttctcccgt cgcgttcgtt ttgtggaagg gaaaccgcta atgctggaag acagctatat
    24241 gccggtaaaa ctgttccgta acctttcgct gcaacatctg gaagggtcga agtttgaata
    24301 tattgaacaa gagtgcggga ttttgattgg cggtaattat gaaagcctga tgccggtgct
    24361 cgccgataga ctgctggcgc ggcaaatgaa ggtagcggaa cacacgccac tgctgcggat
    24421 cacctcgttg tcatatagcg agagcgggga gtttttgaat tattcagtga tgttcagaaa
    24481 tgccagcgaa tactaggtgg agtaccattt acggcgactc cacccggaaa aaagttaacc
    24541 gatactccag aagagcaccg ccagtaattg gggggtgata atgcgcagga acatcactaa
    24601 cggatagaca gtggcgtaag agagcgccgc cgcaccactg gttggatgaa gattattagc
    24661 aaacgccagc gccggaggat cggtcatgga acctgccagc atcccgcaca tggtcaggta
    24721 attcattttg gctaacatcc gcgccagaat gccaacagta atcagcggaa cggcggtgat
    24781 cagggcacca taaccaatcc agcttagccc ttcgccattg accagggtat tcacaaaatc
    24841 cccaccagat ttcagaccaa cgaccgagag gaacagcacg atccccagct cccgcagcgc
    24901 gaggttggca cttggcggca taaaccagta cagcttgccg atactgccga tacgcccgag
    24961 gatcaacgcc ataatcagcg gaccgcccgc cagccccagt ttcaacgcgg ccgggaatcc
    25021 tggcacaaag acgggaatag aacctaacaa tacgccaagc ccgatgccaa taaacactgg
    25081 cagcatctga acctgttgca gtttttgctg cgcattcccc agcacattgg caacggcatc
    25141 aattgcggac ggacgcccca ccaggttgag gatatcgccg aactgcaggc tgatatcgcc
    25201 actggcgacc agttcgaccc cggcacggtt caggcgcgag ataacaacgt catagcgttc
    25261 tttaaagtgc aggtcgcgaa tacgttttcc gagcacgttt tcattggtga ccaccacacg
    25321 ctcgacgcgc aaatcagtgc ctttcgtaga cagcgaggta tcgacctcct gaccaatcac
    25381 cagttgcgca ttatgtaaat ccgctggctg ccccaccaga tgcagcaaat cgcccagttg
    25441 gataatggta tctggcgaag gtacttttag ggtttcttct cgtttcagac gcgagcagat
    25501 aattttgtcg ccgttgagaa tcggtacatc tttaatggct aaatcatgca ggttagggtt
    25561 ctcaacgcga atattgatag tcttgatcag cgcgccgcca ttggtgcgtg aagactcgtg
    25621 ctgctgagct tctgtctcga cattgacgcg gaaaataacc cgcaacatcc acatggtgaa
    25681 caaaatcccg caaatgccga atggatacgc catcgcgtaa ctcatcccca tctgatcgac
    25741 catttccatt ggtgtaccca ggtcgcgcaa gatctgctgc cctgccccca gcgctggcgt
    25801 attggttacc gcaccggaga aaatccccag cactaccggc agtggaatat caaacagttt
    25861 atgcaggatg gcggtaacca gaccaccgat gatgacgatc agaacagcaa acaggttgag
    25921 gcgtaatccg gagacgcgca atgaggcaaa gaagcccggc cctacctgaa tcccgatggt
    25981 ataaacgaac aggatcaggc caaattcctg aataacatgc agcatatcgc tacttaatgt
    26041 catccccgcc tgagaaacaa aatggccgac gatgatccca ccaaacagca cgccgccaat
    26101 acctaatcct atgccgcgaa atttgacgtt gccgataaac aaaccgacga ctgccaccaa
    26161 agccagaata ctgaccgtta atgctatatc actcatcgtc catttctctt gagataacat
    26221 tttaattgct acggattatg gctgaatcgg tgaagcagat ttgccttata gcgcacatta
    26281 tcacgggaaa gaacgtgccg aaatatctta aacagtcgaa atttaacgca ttgttgaaga
    26341 agaaagacaa aaaaagcccc accagaatgg cggggcaaag agaatagcta gttagctatt
    26401 taacgcggga cgttcgctga tagcgatacg ctgcgctgcg atgggttcag gctcattacg
    26461 aattaaatca atatgcagta aaccgtttac gaaggttgcg ccagagactt ccatattttc
    26521 agccagcgta aaactcaggc taaatggctg attcataagc ccttgatgca gccatttttt
    26581 ctcttctttt ggctgctccg gcgtgccttt tacgctcagg cgcgtgcctt ccagttgaat
    26641 ctctaaatct tcctgacgga aacctgccag cgcaagggta atgcggtagt ggttatcgtc
    26701 gcttttctca atgttgtacg gcgggaagct ctggctttca ccggcgtttt gcagtgcgtt
    26761 ggccagtttg tcaaaaccga tccattgacg catcagtggg gataaatcga agttacgcat
    26821 agtcatttct ccttctaaga agcgagtaag tacctgcaaa tccgaagatt cgcatatgct
    26881 ccctgacggc gagcatggag atgtcaggcc gcgccaggcg gccttaggga attagttgat
    26941 ttcgatacgg cgcggttttt tcgcttccgg aatcacgcgt tcgagatcga tatacagcaa
    27001 accatttacc aggttagccc cacgaacatg aatgttctca gctaactgga atttgcgttc
    27061 aaagttgcgt tcagcgatgc cctggtacag ataggtgcgc tctttttgtt cgtcggcgtg
    27121 agcacctttc accaccagca gattatcctg ggcggtaatt tccagttcgc tctcagcaaa
    27181 accagccaca gcgatagcaa tgcggtaatg gttttcgtct accagttcaa cgttatacgg
    27241 agggtagccg ccattactct ggctctggtt gttttctaag tggttaaaca aacggtcaaa
    27301 tccaatagca gaacggtaaa gcggggataa atcaaagtta cgcataatca atagctcctg
    27361 aaatcagcga gaatgtaaga ccttccacaa tggacaggtc aggtagccag aacacccatc
    27421 aggcgcgtcc tcatcgacta cgatgtaaaa tgggtctgga aatgaacttt tcaagcctta
    27481 tcagcggact tttttttgca gtttatggtc tattgcatag actgaggggg cagcacgaaa
    27541 cgttaaagtg cgatagccgc cacagtgcga cgtaatgatg atgttatttt cagcaaggga
    27601 tcgctataac tcatcaggcc aacacccacg atcaacagat gaatttatta tgataagaaa
    27661 tgtgttgtta gcgttcatga tatgcagcgg aatgacatta ctcggaggat gctccagcgt
    27721 gatgtcccat accgggggga aagaaggaac gtatcccggc acccgcgcca gcgccaccat
    27781 gattggcgac gatgaaacca actggggcac taagtcgctg gcgattctcg atatgccgtt
    27841 taccgctgta atggacacgc ttttactgcc gtgggatgtg ttccgcaaag acagctcggt
    27901 gcgctcgcgc gttgaaaaaa gcgaagccaa cgcgcaagcc accaatgcgg ttattccacc
    27961 ggccagaatg cctgataact gatttagcga tcagtttcgg tgatccacag ctggccacct
    28021 tccatccacg ccagccattg accatccggc gagaagacca cggcgtccgc agaaggtgga
    28081 ttggcgtggt tttcggttaa atactcaacc tcgccgcttt gcgcatgggc acaaacaatt
    28141 cgattatcca gcacaaagcc caaccattct cctgacggat gccagttaaa tgcagactga
    28201 atatccgttt tgttatgggt taacttgcgc ggttcgccgc cctgtggcga gataagccac
    28261 agttgcacaa tgccgttatc atcacgcatt aaaaacgcga tttgcgtacc ctgcggatta
    28321 cagcgcaccc agtggcgcgg gacgttgact aaccccggat aagcccgatg atgggtaaag
    28381 gttaaacgtc gctgcacgac gccacgcggt ggcgcgggta gggttgtttc cgttccactt
    28441 aacggcgcat cacctgccga tttccagcta gcttcatctt gcggtaactc aacgataaat
    28501 agctccggca ctttctcgcc ctttggcgaa agtgtgtcgc caataaacgc cagcgcgtga
    28561 tttcctaccc atccttcttc ataagcacga ttgatttcat cactgccagg ctgcggcgtg
    28621 ggcgtggttt tactcaccag cacacaccag tggctaccgc tgtattcacg cggatgttgc
    28681 ttttgtacgt tgaccgggcc aaacggtgca gcaaccccga cgtttcgcaa atccagcgcc
    28741 ggatcgagtt gatgcattac atggtcgtta taggtaaagc tccccctttc accgttcggg
    28801 ctaaagacat gcacatggct gccaccgcgc agcgctcctg gcgtatacgg cgcggtaata
    28861 tccattgcat cgagattgct caccttgccg ccttcagcaa tgactccgcg tcgatgatgg
    28921 aagtcgtaat gccatgtttc atcaggattt tcagggccgt gaataaagac atatttctct
    28981 gacttcggat gaacggtcac cacgccgacg tgtgcgccct gtgacgcgcg atagataacc
    29041 tcgacctcgc ctgtatggat attcacacgc tcaatggtct cgccggtaaa cgacgcccca
    29101 gaaggacgca cgtcaaatac cagccactgg ctgtcgggcg tccaggtatt ggtatttgta
    29161 ttggtattgg tattggtatt ggtgagcagg tgattacggg gagcaaaggt tatctgtttc
    29221 atggcgcggt cctgatacaa aacggttccc gccatcatac ttcacaagga cttcactttc
    29281 agacaatagt cttttgccat cgtgatgaat gtgagtgaaa agatggaaca tttcgacgtg
    29341 gcgattattg gcctcggccc ggcagggtcg gcgttggcac gaaagttagc cggcaaaatg
    29401 caggtgatcg cgctggataa aaagcaccag tgcggtactg aaggtttcag caaaccttgc
    29461 ggcggtctgc tggcaccgga cgcgcagcga tcttttattc gcgatggact gacgcttcct
    29521 gtcgatgtga tcgccaatcc acagattttc agcgtcaaaa ctgtcgacgt cgccgcatcg
    29581 ctcacgcgta actaccagcg aagctatatc aatattaatc gccatgcttt cgacttgtgg
    29641 atgaaatcgc tgatccccgc cagcgttgag gtttatcacg atagcctgtg ccgaaaaatc
    29701 tggcgtgagg atgataaatg gcatgtcatt tttcgtgcag acggctggga gcaacatatt
    29761 accgcccgct atctggtcgg tgccgatggc gcaaactcga tggtgcggcg acatctctac
    29821 ccggatcatc aaatccgtaa atatgtcgct atccagcagt ggttcgcaga gaaacatccg
    29881 gtgccgttct actcctgcat ctttgataat gcgataactg actgttactc atggagtatc
    29941 agcaaagatg gttattttat ctttggcggt gcctatccaa tgaaagacgg tcagacgcgt
    30001 ttcacgacgc tgaaagagaa aatgagcgcc tttcagttcc agtttggcaa agcggtgaaa
    30061 agtgagaaat gcacggtgtt gtttccctca cgctggcagg attttgtctg tggtaaggac
    30121 aacgcctttc tgattggtga agcggcggga tttatcagcg ccagctcgct ggaggggatt
    30181 agctatgcgc tggatagcgc agagattctg cgtgcggtgt tcctgaaaca gccggagaag
    30241 agcaacgccg cttactggcg cgccacacac aaactgcgtt taaaactctt cggcaagata
    30301 gtaaaaagcc gatgcctgac cgcaccggct ttaagaaagt ggattatgcg cagtggtgtg
    30361 gcgcatattc cacagttgaa agattatcca acgcgcttca catcacccac cagcaggatg
    30421 taagagagcg cgccaatcaa cgcgacggcg gagatataaa ccagtgcagg cgcgaaaccg
    30481 taaccctgcg ccaggtaccc caccaccagc ggaacggtga tgccgcccag accaccggcg
    30541 aagttaaaca cgccgccggt taaaccaatc aggcgcatcg gtgccagaga agagaccagc
    30601 gaccaggtaa tcgaagcaaa accgttgccg aagaatgcca gcgccatcag gcacataatc
    30661 atcatcggat cgttggtgta gttagcgccc ataatgcagg tggagatcag caagccgcag
    30721 ataatcggcg ttttacgcgc aaagcccagt gaaaagccct tacgtaccag cagatccgcg
    30781 acccagccag agagcaggac gccgacaaac gccgcgagga atggcaccgt ggtcataaag
    30841 cctgctttca gcgccgtgat tcctttttcc tgggttaaat agttcgggaa ccaggttaag
    30901 aaaaaccaca gtgcagaagc caccgcaaat tgcccaagat aaacgccgat cagtttacga
    30961 tggaacacca gtttccagtc tttggctgtt aacggctgac gcgcctcttt cttcaccggc
    31021 gcatcaccat ccaccagacc gccgccatca cgaatgtaat ccagttcagc tttgctgata
    31081 cctttggtca ggcgcggcgg ctgataaacc ttaaaccaaa tcagcgacca gataatgccg
    31141 ataccaccag tgacaatgaa cacccagtgc cagctcaaca tctcctgaat ccagatcagc
    31201 agcggagtca aaaacgccag accgacaaac tgaccagacg tataaaaacc aacggcggaa
    31261 gcgcgttcat gttccgggaa ccagctggtc accatccggt tattggtcgg gaaagcgggc
    31321 gcttcgaaaa taccggttat cgcacgcaga ccaattaatg acattaatcc cgtggcaaag
    31381 ccttggaata aagtcgccac tgaccaacca aatatcgcaa taaaataagt cacgcgagaa
    31441 cctacgcgat ctaaaaacca accgccgggg atctggcata gcgtataaag ccaggcgaag
    31501 gccgaaaata catagcccat ttccgctttc gtaatgccga actcttcctg aatatgggcg
    31561 gaagccacgg ccaggttagc gcggtcaaca taacaaatga ccaccgtaat aaagatcatc
    31621 accagcgtca gataacgccg acgccccggc tttgctgcat taacgggaat atccatagcg
    31681 agctttctcc agattttggg catagcgaag ccgctcacca tgccctgtaa tttacagagg
    31741 gttatatttt tgtattgctg ttttagtgcc cgatgagggg cttacgtggc aggaattacc
    31801 actctgctac gctgttatct tcatgacgcc agagcggatt acgccagtcc ggggcatttt
    31861 tactgaactc aataacttta gcttcgtcga tttccacacc taagcccggt ttcgttaacg
    31921 gtttaaagaa gccgccaacc atgctgaagt cttctttgtt tttcacaaag tcgagtaact
    31981 ccgcgccttt gttgtaatga atgcccatac tttgttactg aagtaccgcg ttataggaaa
    32041 caaagtcgat atgcaggcaa gccgccagtg caatcggtcc gaacggacag tgcggcgcaa
    32101 gggtcacatc ataggcttct gccattccag caattttgta gcattcggta ataccgcctg
    32161 catgggagag atccggttgc agaatcgaaa taccgcctgc ctccagcacg cgtttaaaat
    32221 cgaaacgtga gaacatgcgc tcacccgccg ccagtggaat atgcgtttgt gccgccaatt
    32281 tcgggtagta ttcggcttgt tccgccagca ccggctcttc aataaacagc gggcgatacg
    32341 gctccagttc tttaatcagc actttcgcca ttggcgcgct gacgcgaccg tggaaatcaa
    32401 gaccaaactc aatctgattg ccaaaagctt cacgaatttg tgccacggtg ttgactgccg
    32461 catctaccgc gcgggagtta tcaattagcc ccagttcttc acaaccgttc agtttgaagg
    32521 tatcgaagcc gatttcgcgc agggttttaa tgccgtcgat aacatccgcc ggacgatcgc
    32581 cgccgaccca actgtaggct ttaattttgt cgcgaaccag gccgcccatc agttgccaga
    32641 ctggcgcatt cagcactttg cctttgatat cccataacgc ctggtcgatc ccggcgatgg
    32701 cgctcatcag gattggaccg ccacgataaa atccggcgcg atacatcact tgccataagt
    32761 cgttgatgcg cgaaggatcc tgaccaatca aatagtcacc cagctcgtga actgccgctt
    32821 ccaccgtacg ggcgcggcct tcaatcacgg gctcgcccca accgaccacg ccttcatcgg
    32881 tttcaatttt caggaacatc cagcggggag gtaaacgata cgtggtaatt ttggtgattt
    32941 tcattgcact gcctctcgat acgccttaac aaatgctgct gcctgctgcg cggtgcgttc
    33001 tacggactgc ccggcgcgat aaagatcgct gcctaagccc gccccaacac aacctgcgtc
    33061 tatccactgc gccaggtttt ctggcgtcac gccgccaacg gcaaagactg cgatgtccgg
    33121 tggtaatacc gcttttaacg ctttgatgta ttgcggacca aaagccgatg acggaaatat
    33181 tttcagcgcc tgcgcgcccg cttcgagcgc ggtaaaggct tcggtcgccg tcgcgcagcc
    33241 ggggcaaacg gtcatgccgt agcccaccgc gcggcggatc acttcactat ggatattggg
    33301 cgtaacgatg agctgacagc ccatcctggc gagcgcatcg acctgttcag gtttcagtac
    33361 cgtacctgcg ccaatcaacg ccttgtcgcc atacgcatca acgatggcgg gaatgctctg
    33421 ctcccattgt ggggaattca gcgggatttc aaccgcgtcg aacccggcgt caatcaccgc
    33481 gccaacatgc gccagcgcct cgtcgggcgt aatgccgcgc aaaatggcga tcagcgggag
    33541 tttagtttgc cactgcatga gcgatgctcc ttataccagc ctgaaatgcc gtgtcgcccg
    33601 ccaccgccgc cacgtcgcaa cccatcgcct gaaaggcttg ctggtagcgc gcggtcagcg
    33661 atgttccggc gacaagggtg atggcgtgtt gatgggtcac atagtcgcgc atactggcaa
    33721 cctctgcgcc aatcaacaaa ccagagagaa attcgctgac ctgttcgcgg ggaagtgttc
    33781 ccagcacatg cgaggcgcga acttcaaaaa gctgcggcaa tatggcgggc gcattaaggc
    33841 cgcgctcaag gccagccgcg aaggcatcgg cagagttttc ctgcggcggc aaacctgcgc
    33901 caatcaatga gtgatttaac agtaaatgat gtaattcacc ggtcatcacg gtgcgaaaat
    33961 cgttgatttg ctggctatcg gcctgcaccc atttgcaatg cgtaccgggc ataacataaa
    34021 gagaggaagg agccagagct cgcgcgccga tcaattgtgt ttcttcgccg cgcatcacat
    34081 tgtggttatc gtcatgagag acgcataatc cgggaataat ccagatattg tcgccaacag
    34141 acgttaattg ttcgccaata gacgaaaaac gggcaggaac agataaatac ggagcaactt
    34201 tccagccgac attgctgccg accattcctg ccatcaccac tggcgtattt tcttcacgcc
    34261 agtcggtcgt gacttctgct aacactgcag ccggagattt tccgttcagg cgcgtgacgc
    34321 ctgcttctga ttgcctgctc tccaggcagt ggtcgccctg ataaagccag gcgcgcagat
    34381 tggtcgatcc ccagtcaatt gcgatgtagc gagctgtcat gtgatttcct ttaaccttcg
    34441 tgtcgagctg gcaatcatgg taagcgccgc ctgctctgcc gcatcgccgt cctgatgccg
    34501 tatcgcatcg aacagcgcct tatgttcctg gagcgtttgc ggcatgttgg cctcatcgcc
    34561 catccaggtt cgttcaaaaa ccgcccgctg cagcgaactg atcgcaatgc taagttgctg
    34621 taacaccggg ttatgcaccg actgcagcac cgcctcgtgg tagcgaatat ccgcttcgtt
    34681 aaacgcttcg cggtcctgat tgttggcaat catctcgttc agcgccgatt caatctgcgc
    34741 cagatcgctg gaggtcgcgc gctctgctgc ccaacgggca atcgccggtt ccaccagatt
    34801 tcgcacttcg ctcatggcac tgataagccg tgggtcgtag tcgttttcca gcacccattg
    34861 cagtacgtca gtgtcgaggt aattccactg gttacgcggt gccacaaacg ccccgcgata
    34921 acgtttcatt tcaatcagcc gcttcgccat cagcgaacgg aacacctcac ggatgatgtt
    34981 gcgcgaggtt gcaaactctt cacagagctc cgcctcagcc ggaagtggcg agccaggtac
    35041 gtatttgccg tgaacgatct gtttacccag cgtaatgaca atgcgatcgg ttttattgag
    35101 agtcatggag agtccttgtg ctctgtatgt tcttctctac tttaccccga tcgatgcata
    35161 acgcggcaac tttgtagtac cagcgtgatg acgttcgcgt ttgccgcgcg cgtaatgtag
    35221 tacaaactta tattgttgcg ctacaattta gatcaaaaaa agaacaacac ataaaaaaat
    35281 gacatgcgtc gggcagaaat ctgaaaaggg atatcaggcg ctaaacagga gggaaagaag
    35341 agtatgcttt caacggctta gctactcgtt taaaggatta atcatgaagt tgaattttaa
    35401 gggatttttt aaggctgccg gtttattccc actggcgctg atgctttcag gctgtatctc
    35461 gtatgctctg gtttcccata ccgcaaaggg tagttcagga aagtatcaat cgcagtcaga
    35521 caccatcact gggctatcgc aggcaaaaga tagtaatgga acaaaaggct atgtttttgt
    35581 aggggaatcg ctggattacc ttatcactga tggtgccgat gacatcgtta agatgctcaa
    35641 tgatccagca cttaaccggc acaatattca ggttgccgat gacgcaagat ttgttttaaa
    35701 tgcggggaaa aagaaattta ccggcacaat atcgctttac tactactgga ataacgaaga
    35761 agaaaaggca ctggcaacgc attatggttt tgcctgtggt gttcaacact gtaccaggtc
    35821 actggaaaac ctaaaaggca caatccatga gaaaaataaa aacatggatt actcaaaggt
    35881 gatggcgttc taccatccat ttaaagtgcg attttatgaa tactattcac ccagaggcat
    35941 tccggatggt gtttccgcag cattactgcc agtgactgtt acactggaca tcattactgc
    36001 accgctgcaa tttctggttg tatatgcagt aaaccaataa tcagtaagcg ggcaaacgcg
    36061 tttatgctgt ttgcccgccc acagattaat tcagcacata cttctcaata gcaaacgcca
    36121 cgccatcttc aaggttggat tttgtgacaa agttcgccac ttctttcacc gaaggaatag
    36181 cgttatccat cgccacaccg acgcctgcat attcaatcat tgcgatgtcg ttttcctgat
    36241 cgccaatcgc catgatttct tccggtttaa tacctaacac gtcggccagt gatttcacac
    36301 ccgtaccttt gttaacgcgt ttatcgagga tttcgaggaa gtacggcgca cttttcagca
    36361 cggtatattt ctctttcact tcctgcggaa tacgcgcgat agcctggtcg aggatggcgg
    36421 gttcatcaat catcatcact ttcaggaact gggtattggg gtccattttc tccgcttcgc
    36481 agaacaccag cggaattgtg gcaacgaagg attcatgcac cgtgtagtag ctgatatcgc
    36541 ggttagcggt gtacagcgtg gtgcggtcca gggcgtggaa atgagaaccg acttcgcgag
    36601 agagtttttc caggaaacga tagtcgtcat agctgagagc ggtttgcgcc acggtgctac
    36661 catcagcggc cttctgtacc agcgcgccgt tataagtaat gcagtagtcg cccggctgtt
    36721 ccatatgcag ctctttcagg tagttgtgca cacctgcata cggacgaccc gtcgttagca
    36781 cgacattcac gccacgggcg cgagctgcgg caatcgcatt tttaacggcg ggtgaaatgg
    36841 tgtgatcggg cagcagaagg gtgccatcca tatcgatagc aatgagtttg atagccatga
    36901 gttcccaagg tagattagtt cctgacccat gctaacgcga ttccgctcaa aaatcagtac
    36961 aacacccgag ggaaaagggg atgcaacgcg ggttgcatgc tccccatttg cttagcggaa
    37021 gagtttccct ttcagcagtt ccatgcctgc ggaaagcaga tcgttattgg cttgtgctga
    37081 cacttcacct tgcggtgaga gcgcatcaat aatcttcggc aattgctctg ccagtaaact
    37141 ggaagccgta ctggtatcca cgccaagttt ttgcccgaga tcggacaccg catttgtgcc
    37201 gagcgccgac tccagttgct cgccgctaac tgattgattt ctctgttgat tactcaacca
    37261 ggttgagaga atggccccta agccgccact ttgcagtttt tccagcagca cctgaatgcc
    37321 accctgctcc tcaacccaac ttaaaatcgc ctgatatttc cccgcatcgc ctttcagaaa
    37381 ggcaccgaca acttcatcaa aaagccccat gataatcacc tgtaaagcgt tacgtgttga
    37441 cccaaaaagt atagatctgc gggatgataa ttgcggattg cagaaataaa aagggcggag
    37501 atgatctccg cccttttctt atagcttctt gccggatgcg gcgtgaacgc cttacccggc
    37561 ctacaaaatc atgcaaaatc aatatattgc aggagtctcg taggactaat aagcgtagcg
    37621 catcaggcac gctcgcatgg ttaacgccat taaatatcaa tattcgccgc tttcagggcg
    37681 ttctcttcaa taaacgcacg acgcggttct acggcgtcgc ccatcagcgt ggtgaacaac
    37741 tggtcagcgg caatcgcatc cttaacggta acgcgcagca tacgacggct ttccgggtcc
    37801 atggtggttt cccacagctg ttccgggttc atctcgccca gacctttata acgctggatg
    37861 gagaggccac gacgggactc tttcaccagc cagtccagcg cctgctcgaa gctggctacc
    37921 ggctgacgac gctcgccacg ttcgataaac gcatcttctt ccagcaagcc acgcagtttc
    37981 tcacccagcg tgcagatacg acgatattcg ccaccggtga taaactcgtg atccagcgga
    38041 tagtcagtat ccacaccgtg ggtacgcacg cgaacaatcg gctcgaacag gttttgttcg
    38101 gcattggtgt ggacatcaaa cttccactgg ctgccgtgct gttctttgtc gttcagttcg
    38161 ctgaccagcg cgttcaccca gcgggtaacg gtctgctcat cagaaaggtc agcttccgtc
    38221 aacgtcggct gatagataag ctctttcagc attgctttcg gataacgacg ctccatgcgg
    38281 ttgatcattt tctgcgtcgc gttgtactca gataccagtt tctctaacgc ttcgccagcc
    38341 aatgccggtg cactggcgtt ggtgtgcagc gttgcgccgt ccagcgcgat agagatctgg
    38401 tactgatcca tcgcttcgtc gtctttaatg tactgttcct gcttgccttt cttcactttg
    38461 tacagcggcg gctgagcgat gtagacgtga ccgcgttcaa cgatttccgg catctgacga
    38521 tagaagaagg tcaacagcag cgtacgaatg tgcgagccgt cgacgtccgc atcggtcatg
    38581 atgatgatgc tgtgataacg cagtttgtcc gggttgtact cgtcacgacc gataccacag
    38641 ccaagcgcgg tgataagcgt cgccacttcc tgagaagaga gcatcttatc gaagcgcgct
    38701 ttctcgacgt taaggatttt acccttcagc ggcagaatcg cctggttctt gcggttacgc
    38761 ccctgcttcg cagagccgcc cgcggagtcc ccttccacca ggtacagttc ggaaagcgcc
    38821 ggatcgcgtt cctggcagtc tgccagtttg cccggcaggc cagctaagtc gagcgcacct
    38881 ttacggcggg tcatttcacg cgcgcgacgc gccgcttcac gggcacgggc agcatcgata
    38941 attttgccaa ccacgatttt cgcatcagtt gggttttcca gcaggtattc cgccagcagt
    39001 tcgttcatct gctgttcaac cgccgatttc acctcagaag aaaccagttt gtctttggtc
    39061 tgggaggaga atttcgggtc cggcactttt acggaaacga ccgcaatcag gccttcacgc
    39121 gcatcgtcac cggtggcgct gactttggct tttttgctgt agccttcttt gtccatgtag
    39181 gcattcaggg tacgggtcat cgccgcacgg aagcctgcca ggtgagtacc gccgtcacgc
    39241 tgcggaatgt tgttggtaaa gcagtagatg ttttcctgga agccatcgtt ccactgcagc
    39301 gccacttcaa cgccaatacc gtctttttcg gtggagaagt agaagatatt tgggtggatc
    39361 ggcgttttgt tcttgttcag atattcaacg aacgctttga tgccgccttc atagtggaag
    39421 tggtcttctt tgccgtcacg cttgtcgcgc agacgaatgg aaacgccgga gttgaggaac
    39481 gacaactcac gcagacgttt cgccagaatt tcatattcga actcggtcac attggtgaag
    39541 gtttcgaggc tgggccagaa acgcaccatg gtgccggttt tttcagtctc gccggtaacc
    39601 gccagcggag cctgcggtac accgtgttcg tagatctgac ggtgaatttt accctcgcgc
    39661 tgaataacca gctccagttt ttgcgacagg gcgtttacta ccgaaacacc aacgccgtgc
    39721 agaccgccgg acactttata ggagttatcg tcaaatttac cgcctgcgtg cagaacggtc
    39781 atgatcactt ccgccgccga tacgccctct tccgggtgaa taccggtcgg aatgccgcgc
    39841 ccgtcatcct gtacagagac agagttatcg gcgtgaatgg tgacgataat ttctttacag
    39901 tgacccgcga gcgcttcgtc gatagcgtta tctaccacct cgaataccat gtggtgcaga
    39961 ccggtgccgt catccgtgtc gccgatatac atacccgggc gcttacgcac cgcatccagc
    40021 cctttcagga ctttgatact ggaggagtca taagaattcg acatcaacgt ttctcgctca
    40081 tttatacttg ggttaatccg ttattttacc cttttccacg gtaaacatct tcgaattttc
    40141 gtccgacatg tctataacgt gttcagcact gatcgcgctg acaaagacct gtgattgcgt
    40201 cgcttttaag cggctggcaa gcaacccgcg acgctcatca tcaagctcag aggcaaaatc
    40261 atctatcagg tagagacacc gccgcccgct ttcacgagtg aggaactctc cttgcgccag
    40321 acgtaaggcg cacatcaaca gcttaagctg cccacgcgat aaggtatctt ccaccggcgc
    40381 accgtcggcg cgaatgcgta agtccgcttt atgcgggcca tgcgcggtgt aggttagctg
    40441 gcgatcgtgt tcaaaattac gttccagcac ctcagcgtac tctgtctctt tctcccagcc
    40501 gcgctggaaa gagaaagtca gagaaaactc agggagaaat tgcttacagg tatcatccat
    40561 atcggccgcg ataccggcgc tatactccgc gcgccaggtg ctgatttgct ccgccagcgg
    40621 gatcagttct ttatcccacg ggcgtagctg ttcgtaacga gtcacctggc gcagcgccgc
    40681 attgcgctgc ttgagcaatc gcttgagatt gctccaggcg gtgaaaaatc caggttcgtt
    40741 gtgaaagcat ccccagtcga ggaatgctct tctgtatttg gggccgccgt tgagtaaagt
    40801 aaacccttct ggcgttatca actgcatcgg catcaggtgc gccagttccg cgactttatg
    40861 cccgtccgta ccgtcaatac ggactttgct gtcgccctgt ttgtctttgg ttaagccaat
    40921 cgccgtctcg cgctcttcgc cctgtaatcg cccgtggaga acaaatgcct cctgctcatg
    40981 gcgaatgacg cgaccaatct gcaaactgcg aaacgcccga ccatggccga gcgtatagat
    41041 ggcttccagc acgctggttt tgccactgcc gttggcacct accagaaagt taaagccggg
    41101 agataaagcg agatccgcgg tttcaatatt gcggaaatcg cggatcaaca agcgggtgag
    41161 ggacattaca gtctcattgg catgacaaca taggcagccg actgtgatgc ggcatcttca
    41221 atctgcacgc tggaaaccga atcggtcagc atcatgcgga cgttttcgca tttcagcgcg
    41281 ttcagaacat ccagcacata gctgacgttg aagccgattt ccatctccgc accgctatag
    41341 gtaacgtcga ggatctcttc cgcttcttcc tgttccgggt tgttggcagt aattttcagc
    41401 tggttttcgc tgacatacag gcgcacaccg cggaatttct cgttagagag aatcgccgcg
    41461 cgagcaaacg cctgcttgag cagatcgcag cctgcttcca gatgtttgtc cgggttcttc
    41521 ggcagaacgc gacggtaatc cgggaagcga ccatccacca gtttcgaggt gaaaataaag
    41581 tcgccaacgt gggcacgaat gttattgctg ccaatctgca cacgcagcag attgtcgccg
    41641 ccgtcgagca tacgcatcag ttcaatcacg cctttacgcg gtacgatcac cgaatggctt
    41701 ggcaaagatt gaccaattgg cattgaacag accgccagac ggtggccgtc ggttgccacg
    41761 gtgcgcagtt cttcaccttc ggtttcaaac agcataccgt ttaagtaata gcgaacatcc
    41821 tgatgcgcca tcgaaaactg ggttgcttca atcagacgct tcatcgtcgc ctgcggcagg
    41881 gtaaattcga cttcgctctg ccagtcatcg aggttcggga aatccgccgc tggcagggta
    41941 gacagcgaaa aacggctacg cccggagcgc accagcatcc gttcaccttc cagctgcacg
    42001 gcaatttccg cgccttcagg cagaccacgg cagatatcaa agaatttgcg cgccggaacg
    42061 gtcgtcgctc ctggctcgtg tggctgaacc agcgcaacgc gtgccaccat ttccatctcg
    42121 agatcagtac cggtcagcga caacgtaccg tcagcaacct gtaacagcag attaccgaga
    42181 atcggtagcg taggacgacc acctaacgga ccgctcacct gttgtagcgg ttttaataaa
    42241 tgctcacgtt ctacggtaaa tttcataggt ttacgatgac aatgttctga ttaaatttga
    42301 aaaatcttct ttgatatcgt ggctctcttc acgcaactgc tcgatcttac ggcaggcatg
    42361 aagcaccgtc gtgtggtcac gcccaccaaa cgcatcgcca atctctggca gactgtggtt
    42421 agtcagctct ttcgctagcg ccatcgccat ctggcgcgga cgcgccaccg agcgggatcg
    42481 acgcttggaa aggagatccg cgactttgat cttgtagtac tccgccaccg tcttctgaat
    42541 attgtcgatg gtgaccagtt tttcctgcaa tgccagcaag tcgcgcagcg cctcacgcac
    42601 gaagtcgatg gtgatcgccc gtccggtaaa gttggcgtta gcaataacgc ggttcagcgc
    42661 cccttccagc tcacgtacgt tagatcgtag acgcttggcg ataaagaacg ccacttcacc
    42721 cggcaaacga atgtcatttt cgtcggcctt tttcatcagg atcgccacac gggtttccag
    42781 ctctggcggt tcgatcgcca cagtcagtcc ccaaccgaag cgggatttca aacgatcctc
    42841 aacgccgttg atctctttcg gatagcgatc cgaggtgaga atgatctgtt gattaccttc
    42901 cagcagggcg ttgaaggtgt ggaaaaactc ttcctgagat cgttctttat tagcaaaaaa
    42961 ctgaatatcg tcgatcagca gtgcatctac ggaacggtag tagcgtttaa actcttcgat
    43021 cgcgttgttt tgcagggctt taaccatgtc ctgaacaaag cgctcggagt gcatataaac
    43081 cactttggca ttcggcttgc gcgccataat gccgttaccc accgcatgca gcaggtgagt
    43141 tttacccaga cccgtgccgc cataaaggaa caacgggtta taggcaccgc cagggttatc
    43201 cgccacctgg caagccgccg cgcgcgccag ttggttagat ttaccttcaa cgaagttatc
    43261 aaacgtgtgt ttgacgttta cgttagaacg ataggtcggt tctgccgggg ccgggacgtt
    43321 atcccaacct gagcgcgtag aaggcgcagc acgttgcggc tgcgtttgcg ccacctgtgc
    43381 aggggccgcg acgttgctcg tcactgccgc ttgtggcgtt tgcgtcaccg gtttggtgcc
    43441 gacttcaaaa cgcagctgtg gggcatccgc tccgcagaaa ctggttagca gtccattgat
    43501 attattaagg tacttgtccc gtacccaatc gaggacaaaa cggtttggcg cgtacagggc
    43561 cagcgtgtta tcgctcagtt ccgcctgcaa tgggcgtatc cacatactga attctgtggc
    43621 tggtaactca tcctgcaatc gggcaagaca ctgctgccaa agcgaaagtg acacggcgga
    43681 ctccactcga acaaaagtcg ataatgacta aggctgaaac attcatgatt gttgacgtac
    43741 gtcgaaaaga ccctgcttga gggtgacgca cgaaccgctg tctgcggtta tatgcccgat
    43801 caagatcctg caaaacgatc gggaccgcgg atcatagcct aaactgcgca agagatcttc
    43861 tgtttctcac agattttccc tatttatcca caggactttc cagaactcgc taagtgtaaa
    43921 cgatcctgcc gcgaggcggg cacgatttac gccgcatatt ggaaaattta atgaccatag
    43981 acaaaaattg gcttaatcga tctaataaag atccaggacg atccttgcgc tttacccatc
    44041 agcccgtata atcctccacc cggcgcgcca tgctggtttc cactggtgtg aggtcgtaca
    44101 ttttccctgc gaaaaggtgc ggaaaagcgc ggtaaataag gaaagagaat tgactccggt
    44161 gtgtacaatt attacaatcc ggcctcttta atcacccatg gcttcggtgt ccatcgtttc
    44221 atttttcggc ggatatccaa taaagccatt gaatttattc aagtttaggt agaaatcgcc
    44281 atgaaacgca cttttcaacc gtctgtactg aagcgcaacc gttctcacgg cttccgtgct
    44341 cgtatggcta ctaaaaatgg tcgtcaggtt ctggcacgtc gtcgtgctaa aggccgcgct
    44401 cgtctgaccg tttctaagta ataaagctaa cccctgagtg gttaagctcg catttcccag
    44461 ggagttacgc ttgttaactc ccagtcaatt cacattcgtc ttccagcagc cacaacgggc
    44521 tggcacgccg caaattacca ttctcggccg cctgaattcg ctggggcatc cccgtatcgg
    44581 tcttacagtc gccaagaaaa acgttcgacg cgcccatgaa cgcaatcgga ttaaacgcct
    44641 gacgcgtgaa agcttccgtc tgcgccaaca tgaactcccg gctatggatt tcgtggtggt
    44701 ggcgaaaaaa ggggttgccg acctcgataa ccgtgctctc tcggaagcgt tggaaaaatt
    44761 atggcgccgc cactgtcgcc tggctcgcgg gtcctgatag ccctcattcg ggtctatcaa
    44821 cgcctgatta gtccgctact cgggccgcat tgtcgtttca ctccaacctg ttcaagctac
    44881 ggaattgagg cattgcgcag gtttggagtg ataaaaggca gttggttgac ggtgaaacgc
    44941 gtattaaaat gccacccttt acaccctggt ggtgacgatc ccgtcccgcc cggaccattt
    45001 gataccagag aacactaacg atggattcgc aacgcaatct tttagtcatc gctttgctgt
    45061 tcgtgtcttt catgatctgg caagcctggg agcaggataa aaacccgcaa cctcaggccc
    45121 aacagaccac gcagacaacg accaccgcag cgggtagcgc cgccgaccag ggcgtaccgg
    45181 ccagtggcca ggggaaactg atctcggtta agaccgacgt gcttgatctg accatcaaca
    45241 cccgtggtgg tgatgttgag caagctctgc tgcctgctta cccgaaagag ctgaactcta
    45301 cccagccgtt ccagctgttg gaaacttcac cgcagtttat ttatcaggca cagagcggtc
    45361 tgaccggtcg tgatggcccg gataacccgg ctaacggccc gcgtccgctg tataacgttg
    45421 aaaaagacgc ttatgtgctg gctgaaggtc aaaacgaact gcaggtgccg atgacgtata
    45481 ccgacgcggc aggcaacacg tttaccaaaa cgtttgtcct gaaacgtggt gattacgctg
    45541 tcaacgtcaa ctacaacgtg cagagcgctg gcgagaaacc gctggaaatc tcctcgtttg
    45601 gtcagttgaa gcaatccatc actctgccac cgcatctcga taccggaagc agcaacttcg
    45661 cactgcacac cttccgtggc gcggcgtact ccacgcctga cgagaagtat gagaaataca
    45721 agttcgatac cattgccgat aacgaaaacc tgaacatctc ttcgaaaggt ggttgggtgg
    45781 cgatgctgca acagtatttc gcgacggcgt ggatcccgca taacgacggt accaacaact
    45841 tctataccgc taatctgggt aacggcatcg tcgctatcgg ctataaatct cagccggtac
    45901 tggttcagcc tggtcagact ggcgcgatga acagcaccct gtgggttggc ccggaaatcc
    45961 aggacaaaat ggcagctgtt gctccgcacc tggatctgac cgttgattac ggttggttgt
    46021 ggttcatctc tcagccgctg ttcaaactgc tgaaatggat ccatagcttt gtgggtaact
    46081 ggggcttctc cattatcatc atcaccttta tcgttcgtgg catcatgtac ccgctgacca
    46141 aagcgcagta cacctccatg gcgaagatgc gtatgctgca gccgaagatt caggcaatgc
    46201 gtgagcgtct gggcgatgac aaacagcgta tcagtcagga aatgatggcg ctgtacaaag
    46261 ctgagaaggt taacccgctg ggcggctgct tcccgctgct gatccagatg ccaatcttcc
    46321 tggcgttgta ctacatgctg atgggttccg ttgaactgcg tcaggcaccg tttgcactgt
    46381 ggatccacga cctgtcggca caggacccgt actacatcct gccgatcctg atgggcgtaa
    46441 cgatgttctt cattcagaag atgtcgccga ccactgtgac cgacccgatg cagcagaaga
    46501 tcatgacctt tatgccggtc atcttcaccg tgttcttcct gtggttcccg tcaggtctgg
    46561 tactgtacta tatcgtcagc aacctggtaa ccattattca gcagcagctg atttaccgtg
    46621 gtctggaaaa acgtggcctg catagccgcg agaagaaaaa atcctgattc ggtgagtttt
    46681 cgctaaaata agggcggtca gttgaccgcc ttttttcttt tcgtagggcg gataagcacc
    46741 gcgcatccgc cacacaaagc aacaggaaca tcatgagcga taatgacact atcgtagccc
    46801 aggccacgcc tccgggacgt ggcggcgttg gcatcctgcg catctccggc ttcaaagccc
    46861 gtgaagttgc cgaaaccgtg ctgggtaaac tgcctaagcc gcgctacgcc gattatcttc
    46921 cgtttaaaga cgccgacggc agcgtgctcg atcaggggat tgcgctatgg ttccctggcc
    46981 cgaactcgtt caccggcgaa gatgtgctgg aactgcaagg tcatggcggt ccggtgatcc
    47041 tcgacctgct gttaaaacgc attctgacca ttcccggcct gcggattgct cgccctggtg
    47101 agttttccga acgcgcgttt cttaacgata aacttgactt agcccaggcc gaggcgattg
    47161 ccgatcttat cgacgccagt tcggaacagg cggcccgttc ggcacttaac tcgctgcaag
    47221 gcgcattctc cgcacgggtt aatcatctgg tagaagccct cacccacttg cgcatttacg
    47281 tcgaagcggc aattgatttc cctgatgaag agatcgattt cctctccgac ggcaaaattg
    47341 aagcccagct taatgacgtg atggccgatc tcgatgcagt gcgtgctgaa gcacgtcagg
    47401 gtagtttgtt gcgcgaaggg atgaaagtag tgattgccgg acgtcctaat gccggtaaat
    47461 cgagcctgtt aaacgcgctg gcgggccgtg aagcggcaat cgtaaccgat atcgccggaa
    47521 ccacgcgtga cgtgctgcgt gagcatatcc acattgacgg aatgccgctg catatcatcg
    47581 acactgccgg gctacgtgag gccagtgacg aagtggaacg tattggtatc gagcgcgcgt
    47641 ggcaggaaat tgaacaggcc gaccgcgtgc tgtttatggt cgatggcacc acgacagacg
    47701 ccgttgatcc ggcagagatc tggccggaat ttatcgcccg tctgcccgcg aaactgccga
    47761 tcaccgtggt acgcaataaa gccgatatca ccggcgaaac gctgggaatg agtgaagtga
    47821 acggtcacgc gttaattcgt ctctcggcaa ggactggtga aggcgtggac gtgctgcgta
    47881 accatctcaa acagagcatg ggctttgaca ccaatatgga aggcggcttc ctggcgcgcc
    47941 gtcgccactt acaggcgctg gaacaggcag cggagcattt gcagcaaggt aaagcgcaac
    48001 tgttgggagc ctgggcaggt gaactgctgg cggaagagtt gcgcctggca cagcagaact
    48061 taagcgaaat caccggggaa tttacttcag acgacctgct ggggcggatt ttctccagct
    48121 tctgtattgg taagtaaccg cgcttacgaa gccgtattct gactgtcaga tgcggcttcg
    48181 cttcattgtt agcactcctg ttatgcctca accctttttt aaacattaaa attcttacgt
    48241 aatttataat ctttaaaaaa agcatttaat actgctcccc gaacgattgt gattcgattc
    48301 acatttaaac aatttcagaa tagacaaa
//
LOCUS       DAJTSA010000022        47956 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000022 DAJTSA010000000
VERSION     DAJTSA010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.022"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO369_001326"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO369_001326"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR5981661.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="OO369_001327"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="OO369_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR5981662.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO369_001328"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO369_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR5981663.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO369_001329"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO369_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR5981664.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO369_001330"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO369_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR5981665.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="OO369_001331"
     CDS             5377..5835
                     /locus_tag="OO369_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR5981666.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="OO369_001332"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="OO369_001332"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR5981667.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="OO369_001333"
     tRNA            6887..6971
                     /locus_tag="OO369_001333"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="OO369_001334"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="OO369_001334"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO369_001335"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO369_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR5981668.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO369_001336"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO369_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR5981669.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO369_001337"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO369_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5981670.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="OO369_001338"
     CDS             complement(10252..13995)
                     /locus_tag="OO369_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR5981671.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO369_001339"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO369_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR5981672.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="OO369_001340"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="OO369_001340"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR5981673.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="OO369_001341"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="OO369_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR5981674.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO369_001342"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO369_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR5981675.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="OO369_001343"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="OO369_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR5981676.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO369_001344"
     CDS             complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO369_001344"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR5981677.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO369_001345"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO369_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR5981678.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21887..22987
                     /gene="chaA"
                     /locus_tag="OO369_001346"
     CDS             21887..22987
                     /gene="chaA"
                     /locus_tag="OO369_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR5981679.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23392..23499
                     /locus_tag="OO369_001347"
     CDS             23392..23499
                     /locus_tag="OO369_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5981680.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO369_001348"
     CDS             complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO369_001348"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR5981681.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24538..25347)
                     /locus_tag="OO369_001349"
     CDS             complement(24538..25347)
                     /locus_tag="OO369_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR5981682.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25351..25743)
                     /locus_tag="OO369_001350"
     CDS             complement(25351..25743)
                     /locus_tag="OO369_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR5981683.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO369_001351"
     CDS             complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO369_001351"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5981684.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO369_001352"
     CDS             complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO369_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR5981685.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO369_001353"
     CDS             complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO369_001353"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR5981686.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29167..29790
                     /gene="lolB"
                     /locus_tag="OO369_001354"
     CDS             29167..29790
                     /gene="lolB"
                     /locus_tag="OO369_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR5981687.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29790..30641
                     /gene="ispE"
                     /locus_tag="OO369_001355"
     CDS             29790..30641
                     /gene="ispE"
                     /locus_tag="OO369_001355"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR5981688.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30792..31739
                     /gene="prs"
                     /locus_tag="OO369_001356"
     CDS             30792..31739
                     /gene="prs"
                     /locus_tag="OO369_001356"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR5981689.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31864..33543
                     /gene="dauA"
                     /locus_tag="OO369_001357"
     CDS             31864..33543
                     /gene="dauA"
                     /locus_tag="OO369_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR5981690.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO369_001358"
     CDS             complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO369_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR5981691.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34154..34738
                     /gene="pth"
                     /locus_tag="OO369_001359"
     CDS             34154..34738
                     /gene="pth"
                     /locus_tag="OO369_001359"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5981692.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34855..35946
                     /gene="ychF"
                     /locus_tag="OO369_001360"
     CDS             34855..35946
                     /gene="ychF"
                     /locus_tag="OO369_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR5981693.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36790..39657
                     /locus_tag="OO369_001361"
     CDS             36790..39657
                     /locus_tag="OO369_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5981694.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="OO369_001362"
     CDS             complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="OO369_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR5981695.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41904..42974
                     /gene="dhaK"
                     /locus_tag="OO369_001363"
     CDS             41904..42974
                     /gene="dhaK"
                     /locus_tag="OO369_001363"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR5981696.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42985..43617
                     /gene="dhaL"
                     /locus_tag="OO369_001364"
     CDS             42985..43617
                     /gene="dhaL"
                     /locus_tag="OO369_001364"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR5981697.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43628..45046
                     /gene="dhaM"
                     /locus_tag="OO369_001365"
     CDS             43628..45046
                     /gene="dhaM"
                     /locus_tag="OO369_001365"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR5981698.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45366..47063
                     /gene="treA"
                     /locus_tag="OO369_001366"
     CDS             45366..47063
                     /gene="treA"
                     /locus_tag="OO369_001366"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5981699.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(47142..47581)
                     /locus_tag="OO369_001367"
                     /pseudo
     CDS             complement(47142..47581)
                     /locus_tag="OO369_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        12041 a        12342 c        11743 g        11830 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgctgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct atagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggc ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgacca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgtggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatagt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt taccgctcat
    21301 tttgaaacca ctaacggaac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    21781 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    21841 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    21901 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggtgctggt
    21961 agtgctgttc ctgtggggaa gtagccagac actaccagtg gtcattgcca tcaatcttct
    22021 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    22081 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    22141 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    22201 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    22261 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    22321 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    22381 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    22441 tttgctgatc caggccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    22501 tgatgacgac gacccgcacc acggtaaaac gtctgcccat agcagcctgt ggcatgctat
    22561 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagctcgct
    22621 ggagacattg ctcgacagca tgaatgcccc tgttgccttt actggcttcc tggtggcact
    22681 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggttca
    22741 gcgcgcgatg aatctgttct ttggttcagt gttagcaaca atttcgctta ccgtacctgt
    22801 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    22861 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    22921 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    22981 tgcctgacag ttatatctcc tccgatgacg gaaatcggga gagataagat aaattcctgc
    23041 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    23101 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    23161 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    23221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    23281 aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc
    23341 agacacttca agaatagccg caaaatgttg tcattacaac aaggcggcta tatgacgctc
    23401 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
    23461 gccgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
    23521 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc ggcctttttt
    23581 gtatctggca cccatgaaaa aagtcttaac gcagaacgct aagactttat ttttcaagca
    23641 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    23701 tcatctgctt gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    23761 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    23821 cggctcgtgc cagctcagcc acctgagcac gacgaccacc ggaagctgcg ccaaacggat
    23881 cgcggcattg cagtgcgtgg gtcacgtcga aaatcaccgg cgagttacca gacactttct
    23941 tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag ttagcaccac
    24001 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    24061 tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg gttttcgcca
    24121 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    24181 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    24241 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    24301 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    24361 gtttctgggt cacagttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    24421 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    24481 tgctaaccac tttttgtttc ataggatcgc cttaatcttg agtaaaatgt cgattaatta
    24541 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    24601 cgggtcttcc ggacaccgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    24661 atccagttgc gcataaatca acccacgatc gcgaatttca tagggatctt cagggttgaa
    24721 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    24781 ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    24841 ttcataaaac agttccgccg acgggctgat atttcccttt aaccatccgt ccagcatatg
    24901 ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat ccggacattc
    24961 aatgcgcaat atcagctgcg tagggaaaat caccggcagc agcggcaaat cgagacgatt
    25021 cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat tctttaacac
    25081 ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt taaatcccca
    25141 gtcgccgtaa aacagcgcga tcagtttttc cagctgctct tctaaaggca gaagctggct
    25201 gatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat cttgcgaggg
    25261 aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg gcgctttatt
    25321 aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt ttgtggtggc
    25381 gagtttaatg atgatgtaca gcaccaccaa cgccagcggg aaggcaataa tgcgcgcctg
    25441 ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacgatat aaatgataac
    25501 tccaaacagc ttttcagtca gccactgtgc ctgcccggaa aatggcagga tgtgcgcttt
    25561 agccatcaac gcaatgccgc ttaacaataa caccgtatcg acaaccggcg gcacaatgcg
    25621 cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac gtaaggttaa
    25681 tagcccaaca gaaagcgcga tactaataag atgaacacta agcagtgtag aaaaacttgt
    25741 cattgataat agcggccgag cgttacgcgc tcgttatcac catagtcacg gcaggtttcg
    25801 acgtcatgat accccgcgag gataaatgct tgtcgcaccg cttcgccctg ctgccagcca
    25861 tgttccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc gatgatatgc
    25921 acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc aaagcggaca
    25981 tcgccttgtt gaagatgtgg gtcctgctcg tcaatatacg gcggattgct gacaatcatc
    26041 gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg
    26101 atcgccagat gctgggcatt acgttgtgcc agggagacag catcaggcat acgatctaca
    26161 gcgataattt cgcagtccgg gcgctcgcta gccagcgcaa gcgcaatcgc cccggtaccc
    26221 gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc
    26281 agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa cggcaacgac
    26341 cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg acgtgtcagt
    26401 agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata
    26461 aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg ccgcgggctt
    26521 tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc cattattcct
    26581 gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt tcaatcagca
    26641 tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg atgcggtgat
    26701 cggtaacgcg cccctgcggg aaattgtaag tacggttacg gtcgctgcga tcgccactcc
    26761 ccagcaggtt acgacgggta gacgcttcgg cctgttggcg ttttgccatt tcagcagcgt
    26821 ggatgcgagc accgagcact gaaagcgcct tcgctttgtt tttatgttgt gaacgttcgt
    26881 cctgacattc aacaacaatc ccggtcggca agtgagtaat acgaattgcc gaatcggtgg
    26941 tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt atcaatgcgt aaatctgctg
    27001 ggttgatgtc cggcagttct gcgtcaggca gttctggcat taccgcaacg gtacaagcag
    27061 aagtatgaat acgaccctgc gattccgtag caggaacacg ttgcacgcga tgaccgccgg
    27121 attcaaattt cagacgacca tacacaccat caccgctaat tttggcgatg atctctttat
    27181 aaccaccatg ttcaccctcg ctggcgctca tgatttctac ccgccagcgg cgggcttcgg
    27241 cgtaacggct gtacatacgg aacagatcgc ctgcaaacag cgctgcttcg tcgccgccgg
    27301 ttccggcacg gacttcgagg aaggcgttac gttcgtcatc aggatctttt ggtagtaata
    27361 gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt agcttcgcgc agttcatcct
    27421 gcgccatctc acgcatttca ggatcgtcga gcatcatctg tgcggtttcg atatcttcct
    27481 gaacctgttg ccagtcggta aaacagcgcg aaacatcact taactgcgca tattcgcgtg
    27541 ataatgcgcg aaaacgttcc tggtcggcga tagtttgcgc gtcacccagc aacgcctgaa
    27601 cttcttcatg gcgttcatgc agggcttcca gtttggcaac gatagaaggc ttcataggcg
    27661 taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac tccagcccga ggctgtcgcg
    27721 cagaatattc aggcgttcgt tatccccgtc acgggcggcc tgttgaagtg atttcgttgg
    27781 cgcatggatc aagcggttag tcagtttcca tgccagatcc tgcataatgg cttgcgcgtc
    27841 gccgccctgc tcaagggccg ctaacgcttt ggcggttaac tcatcgcgaa cgtgctctgc
    27901 ctggctgcga tactcgcgaa tggtttcgct ggcgctttgt gctcgcagcc acgccataaa
    27961 ttcgctggtt tcctgagcga caatagtttc cgcctcaacc gctgcggctt tacgctgcgc
    28021 caggttgtgc gaaatgatgc tttgcagatc gtccacacta taaagataag cattcgccag
    28081 tttgccaact tccggctcaa catcgcgcgg aacggcaata tccaccaaca gcattggttg
    28141 gttacgacga ctttttaatg cgcgctccac catgcctttc ccgataatcg gtaacgggct
    28201 ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt tcgtcgatct cactcagggc
    28261 aatcacttcc gcgccaactt catctgccag aatttgggca cgttcgcgag tgcggttggc
    28321 gataatcatc ttctgtactt tatgttcgcg cagatgacgc gctaccagct cgatggtttc
    28381 gcccgcgcct accagcaaca ctgtgaccgt agagagcgat tcaaagatct gccgcgccag
    28441 cgtacaagcc gcaaaagcga cagacacagc actggcaccg atatctgttt cagtgcgaac
    28501 gcgtttcgct acagagaaag atttctggaa catgcgttcc agttcgctgg ccttcatatg
    28561 acctttttgc gaatcgacaa acgctttttt aacctgaccg aggatctgcg gctccccaag
    28621 aaccagtgaa tccaggccgc tggcaacacg cattaaatgg ctaaccgcgt cgttatcctg
    28681 atgccagtag aggcttttac gcagatcttc ttcattaaga ttgtgataat cgcaaagcca
    28741 gcggattaac gcctcttgca ggttatcctg ctcttcaacg ctaagataaa gttccgtgcg
    28801 gttgcacgtc gacagcacca cgccgccctg caccatcggc tgcgcaagca ggctgtcaag
    28861 cgcctgatcg agcttatccg gcgaaaacga tacacgttct cgcagcgata caggtgccgt
    28921 tttatggttg ataccgagtg ctaaaagggt catgtctgcg ggaaataata ccaacgttga
    28981 tagggttagt ctgcttgcat catacaggat gcgtaagatc aataaaagag agcgccccct
    29041 tttggagtaa ttgccgaaag ccgttagatt ctggcaatta agacaacttg aacatagacg
    29101 atagcggacg gtaacgctag cattaagggt tataactgca acgtatctca aggacttgtc
    29161 atcactatgc ccctgcccga ttttcgtctt atccgcctgc taccgctggc tgctcttgtg
    29221 ctcactgcct gttccgttac cacgcccaaa ggtcctggca aaagcccgga ttcgccacaa
    29281 tggcgtcagc atcagcaaga cgtgcgcaat cttaatcagt atcagactcg cggcgcgttc
    29341 gcttatattt ctgaccaaca aaaagtgtac gcccgctttt tctggcagca aaccggccag
    29401 gatcgctacc gtctgctgct cactaaccca ttgggcagca cggaactgga gctgaatgct
    29461 caaccgggta acgtgcagtt agtcgacaat aaaggtcagc gttataccgc cgatgacgcc
    29521 gaagagatga ttggcaaatt gaccggaatg ccaattccgc tcaacagctt gcgccagtgg
    29581 attttaggtt taccgggtga tgcaaccgac tacaaactgg acgaccagta ccgcctgagc
    29641 gaaattacct acagccagaa tggcaaaaac tggaaggttg tttatggtgg ttatgacacc
    29701 aaaacgcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca acgcatcaag
    29761 ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct ccggcaaaac
    29821 ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc
    29881 tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac gatggggata
    29941 ttcgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag
    30001 cgcggttgtt gatgaaaact gcggcagaca gcgggcgtct tccgacggga agcggtgcga
    30061 atatcagcat tgacaagcgt ttgccgatgg gcggcggtct cggcggtggt tcatccaatg
    30121 ccgcgacggt cctggtggca ttaaatcatc tctggcaatg cgggctaagc atggatgagc
    30181 tggcggaaat ggggctgacg ctgggcgcag atgttcctgt ctttgttcgg gggcatgccg
    30241 cgtttgccga aggcgttggt gaaatactaa cgccggtgga tccgccagag aagtggtatc
    30301 tggtggcgca ccctggtgta agtattccga ctccggtgat ttttaaagat cctgaactcc
    30361 cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa atgtgaattc agcaatgatt
    30421 gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc ggtgctttcc tggctgttag
    30481 aatacgcccc gtcacgcctg actgggacag gggcctgtgt ctttgctgaa tttgatacag
    30541 agtctgaagc ccgccaggtg ctagagcaag ccccggaatg gctcaatggc tttgtggcga
    30601 aaggcgttaa tctttcccca ttgcacagag ccatgcttta agccgggcaa gctgagtttc
    30661 ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc tttaatacac cgcctggaaa
    30721 ggatcatgcc tggcccgcac agttttcggc agattctttc caccaatgga cgcatgcctg
    30781 aggttcttct cgtgcctgat atgaagcttt ttgctggtaa cgccaccccg gtactagcac
    30841 aacgtattgc caaccgcctg tacacttcac tcggcgacgc cgctgtaggt cgctttagcg
    30901 acggcgaagt cagcgtacaa attaacgaaa atgtacgcgg tggtgatatt ttcatcatcc
    30961 agtccacttg tgcccctact aacgacaacc tgatggaatt agtcgttatg gttgatgccc
    31021 tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc ctactttggc tatgcgcgcc
    31081 aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc gaaagtggtt gcagacttcc
    31141 tctccagcgt cggtgttgac cgtgtgctga cagtggatct gcacgctgaa cagattcagg
    31201 gtttcttcga cgttccggtt gataacgtat ttggtagccc gatcctgctg gaagacatgc
    31261 tgcagctgaa tctggataac ccaattgtgg tttctccgga catcggcggc gttgtgcgtg
    31321 cccgcgctat cgctaagctg ctgaacgata ccgatatggc aatcatcgac aaacgtcgtc
    31381 cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga cgttgcaggt cgtgactgcg
    31441 tactggtcga tgatatgatc gacactggcg gtacgctgtg taaagctgct gaagcgctga
    31501 aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca cccgatcttc tctggcaacg
    31561 cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt tgtctgcgat accattccgc
    31621 tgagcgatga aatcaaatca ctgccgaacg tgcgtactct gaccctgtca ggtatgctgg
    31681 ccgaagcgat tcgtcgtatc agcaacgaag aatcgatctc tgccatgttc gaacactaat
    31741 cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc catttgtatg
    31801 acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg atgaaacatt
    31861 attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat cgacgtttgc
    31921 tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg gataaccgtc
    31981 gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt ggccccccag
    32041 tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctaacggg tgggtcacgc
    32101 tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt ttcgcaacag
    32161 tttggactgg caggactgct ggttgcgacc ttgttgtcgg gaatcttttt gattctgatg
    32221 gggctggcac gctttggtcg tctgattgag tatattccgg tttccgtcac cttaggtttc
    32281 acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct cggtctgcaa
    32341 atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat ggcgctgccg
    32401 accattaatg tgggtgatgc tgccattggc attgtgacgc ttggcattct ggttttctgg
    32461 ccgcgtctgg gcattcgttt acccggtcac cttccggcct tgctggctgg ttgcgcggtg
    32521 atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca attccactac
    32581 gtcctggccg atggttctca gggtaacggt attccgcaac tgctgccgca actggtgctg
    32641 ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg caccctgctg
    32701 cctgcggcat tctcaatggc aatgctcggg gcgatcgaat ctctgctctg cgctgtggta
    32761 ctggatggta tgaccgggac taagcacaag gcgaatagtg aactggtcgg tcaggggctg
    32821 gggaatatca tcgctccatt ctttggtggt attaccgcta ctgctgccat cgcgcgttct
    32881 gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca ctctattctg
    32941 gttattctcg ccctgctggt actggcacca ctgctctcct ggctgccgct ttccgccatg
    33001 gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcacaaagt ggtcgacttg
    33061 ctgcgtcatg cgccgaaaga tgacatcatc gtcatgctgc tgtgtatgtc actgaccgtg
    33121 ctgtttgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct gctgtttatg
    33181 cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagatgttcc agacgatgtc
    33241 ctggttctgc gcgttattgg cccgctgttt tttgctgctg ctgaaggctt attcacggac
    33301 ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc cgttccggta
    33361 cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc cgaaggatgt
    33421 gaactgcgcg tgtgcaacgt ggaattccag ccactgcgaa ctatggctcg cgcaggcatt
    33481 caaccgatcc cgggacgcct cgcgttcttc ccgaatcgtc gcgcggcgat ggcggattta
    33541 taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca ttatttgtta
    33601 gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtgacgaa cccaccagta
    33661 acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga gaatggcacc
    33721 gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa actgtggtaa
    33781 ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca accccatgag
    33841 cacgttaccg agtatcgaag cgtttttgcg tttcatatgt cacctccgga actttctggg
    33901 ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc ctgtgggtta
    33961 tgtgcgggcg taatcacaat tacaaccctt atttcaacaa aactttacaa ataaacgcct
    34021 gaacaccttg actttctaat agtccactca ggcaattatt acgttatctg catttgatac
    34081 gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat gtacacactc
    34141 aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata
    34201 cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag agcgtttgcg
    34261 cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca cccttggaag
    34321 cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca aagccgttgc
    34381 ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtggccc acgacgaact
    34441 ggatctgcct cctggcgtcg ccaaatttaa gttgggcggt ggccatggtg gtcacaatgg
    34501 actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt tacgcatcgg
    34561 aatcggtcat ccgggcgata aaaataaagt tgtcggtttt gtgttaggca aaccgcctgt
    34621 tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta ctgaaatgtg
    34681 gtttacagat ggcttgacca aagcaacgaa ccgattgcac gcctttaaag cgcaataagt
    34741 cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat ttccatttct
    34801 gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta aatcatggga
    34861 ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt caacgcgctg
    34921 accaaagccg gtattgaagc ggccaacttt ccattctgca ccattgagcc gaacacaggc
    34981 gtcgtaccaa tgcctgaccc tcgcctggat caactggctg aaatcgtaaa accgcaacgt
    35041 acgcttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg cgcatcgaaa
    35101 ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc gatcggtcac
    35161 gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt taacccggct
    35221 gacgatattg aagttatcaa caccgaactg gcgctggcag acctcgacac ctgcgaacgt
    35281 gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa agctgagctg
    35341 gcggtcctgg aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg cgcgctggat
    35401 ttaagcgctg aagagaaagc ggctattcgt tacctgagct tcctgacgct gaaaccaaca
    35461 atgtacatag ccaacgtcaa cgaagatggt tttgaaaaca acccatatct tgaccaggtg
    35521 cgtgaaatcg cggcgaaaga aggttctgtt gtggttccgg tttgtgctgc tgttgaagca
    35581 gacattgccg aactggacga cgaagaacgt gacgagttta tgcaggagct tgggctggaa
    35641 gaaccgggcc tgaaccgtgt gatccgtgcc ggttataagc tgctgaacct gcaaacttac
    35701 ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggagc aaccgcgccg
    35761 caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc acaaaccatc
    35821 tcgtttgaag atttcatcac ttacaaaggt gaacaaggcg cgaaagaagc aggcaaaatg
    35881 cgtgcagaag gtaaagatta catcgtgaaa gatggcgatg tgatgaactt ccttttcaac
    35941 gtctaattaa tttattgtct catgaggttt cgctaaatct catgaagagc gcaaaagtat
    36001 ataaaatcca cgcaattgcg tggatttttc atttcaccga gtcctaatca ttcttgtaac
    36061 atcctacact tttgtgctta ttgcctacag tgaaccacga gaataattaa agacataagt
    36121 gttcagatga ctgatatctc attagcagtg atataacgtc agtttttgtt ccactacaca
    36181 tcagccctct tcacagctga tcaatattga tctcgctcca atatgctggc gagaaaagga
    36241 cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt acgtgcgatc
    36301 ctggcaagat gaaaatgata accagtagca caataataac gataggccag aaaatccatt
    36361 gaacaaaaat gaatatcgat gttttttgct ttttcttatt agtaatgcaa aaatggcgca
    36421 tattgttttt gttatgcaga tacatcaagc gaaaatccca taactaatta ggattattac
    36481 aaaaaatctt caccaaattt tttaaccgct gtttgttgta atatttttgc catattcata
    36541 gtaatgaatt ggaataagta acgacaactt agggaataac tcgatataaa ctcacctttg
    36601 taaggggggg gttaaatata tattaattcc tgtgttcctg attttgcccg gtaatagaga
    36661 attattctca gagaagctca tcatgattgc accatacaga tatcatgatg ataagaggat
    36721 attattcatt gacttaaaca aaatgacaaa atccatagac ccatagcctg ggtactatat
    36781 aggagtggta tgggcatcaa acaacacaat gggaatacca aagccgatcg tctcgctgaa
    36841 ttaaaaatcc gttcgccctc aattcaactg ataaaatttg gcgctattgg tttgaatgca
    36901 attatctttt cccccctgct gatagctgct gatacaggaa gtcaatatgg caccaatatt
    36961 actattaatg atggtgacag aattacagga gataccgccg atccatcagg aaacctctat
    37021 ggtgtaatga ccccagcagg aaacacgcct ggcaatatca acctgggtaa tgatgtcacc
    37081 gtcaatgtca acgacgcctc tggatatgca aaaggaatca ttattcaggg caaaaacagc
    37141 tccctgacag ctaaccgact cacagtagat gttgttggtc aaacctctgc catcggcatt
    37201 aacttaattg gtgactatac ccatgctgac ttaggcacag gcagcaccat taagagtaac
    37261 gatgacggca tcattattgg gcatagctca acactaacag ccactcaatt caccattgaa
    37321 aactcgaacg gtataggcct aaccatcaat gactatggca ccagtgtcga tcttggaagc
    37381 ggaagtaaaa tcaagaccga tggaagtaca ggtgtttata tcggtggtct caacggcaat
    37441 aacgccaatg gtgctgcgcg ttttacggcg acagacctga caatcgatgt tcagggctac
    37501 agcgccatgg ggataaacgt acagaaaaac tctgttgtcg atctcggaac aaacagtacc
    37561 attaaaacca atggcgataa tgctcacggc ctctggagct ttggccaggt tagcgcgaat
    37621 gcactcactg ttgatgtaac tggagccgcg gccaatggcg tcgaagttcg tggtggtaca
    37681 accactatcg gtgcagatag ccatatttct tccgcgcagg gcggtggcct cgtcaccagt
    37741 agttcagacg cgacaatcaa tttttctggc acggcagcgc aacgaaacag catcttttcc
    37801 ggcggttctt atggtgcctc ggcccagacg gcaacggctg ttatcaacat gcaaaatacc
    37861 gatattacgg ttgatcgtaa tggcagtctg gcgctgggtt tgtgggcgct cagcggcggt
    37921 agaataaccg gagacagttt ggctatcacc ggcgcggcag gagccagagg gatttatgcc
    37981 atgaccaaca gccagatcga cctcacgagc gatctggtca ttgatatgag tacacccgac
    38041 cagatggcca tcgcaacgca acatgacgat ggttatgccg ccagccgcat caacgcctcg
    38101 ggtcgtatgc ttatcaacgg tagcgttctt tccaaaggtg ggctaatcaa tctggatatg
    38161 caccctgggt cggtttggac aggttcctcc ctcagcgata atgtcaatgg cgggaaactg
    38221 gacgttgcaa tgaataacag cgtctggaac gtaacaagta attctaatct cgacacgctg
    38281 gcgctgagcc attcaactgt cgattttgcc agccacgggt caactgccgg cacatttacc
    38341 acattaaacg tagagaacct gagcggtaac agtaccttta ttatgcgtgc tgatgttgtt
    38401 ggcgagggta atggcgttaa taatagaggg gatttattga atatcagcgg gagtagtgct
    38461 ggtaatcacg tattggctat ccgcaaccag ggcagcgagg ccacaacggg aaatgaagtt
    38521 ctgacagtgg taaaaaccac tgacggcgcg gcctcgttca gcgcgtcttc tcaggttgag
    38581 ttggggggat atctgtacga tgtgcgtaaa aatggcacta actgggagct ttacgcttcc
    38641 gggacagttc cggaaccgac tcctaatcct gaacccacac cagctcccgc tcagcctccc
    38701 atagtcaacc ccgatcctac gcctgaaccc gctcccacgc ctaaacccac cacgaccgca
    38761 gatgctggcg gcaattatct caatgtcggt tacttattga actatgttga aaaccgtacg
    38821 ctgatgcaac ggatgggtga cttgcgaaat cagagtaaag acggtaatat ctggttgcgc
    38881 agttatgggg gaagcctgga ctcctttgcc agtggcaaac tgagcggctt tgacatgggt
    38941 tacagcggta tccagtttgg tggggataaa cgtctctctg atgtaatgcc gttgtatgtc
    39001 ggtctgtata ttgactcaac acatgcatcg ccggactata gcggaggcga cggtaccgca
    39061 cgttcagact acatgggaat gtacgccagt tacatggcac aaaacggttt ttacagcgat
    39121 ctcgttataa aagcatcgcg ccagaaaaat agtttccacg tactggacag tcagaacaac
    39181 ggcgttaacg ccaacggcac tgcgaatgga atgagcatct ccctggaagc cgggcagagg
    39241 ttcaacctgt cccctactgg ttatgggttc tatatagagc cgcaaaccca gcttacatac
    39301 agccaccaga atgagatggc tatgaaggcg agtaatggcc tcaatataca tctgaatcac
    39361 tacgaatcgc tgctggggcg tgccagcatg atactggggt atgacatcac cgcaggcaac
    39421 agccagctga atgtctatgt gaagactggc gctatccgcg agttttcagg ggataccgaa
    39481 tatctgttga acgactcccg ggagaagtac agtttcaaag gtaatggctg gaataacggc
    39541 gtgggagtca gtgcacagta taacaaacag cacacattct atctcgaagc ggattacacg
    39601 cagggtaacc tctttgatca gaagcaagtc aacggaggat atcgcttcag cttttaagtg
    39661 cgggatagac aagcccttaa acgatgctga aaattgttag cgtgcgccgg aatgaaatac
    39721 agatcactct gatgtatcag aaatcgtttc tgtctttcat acccggcgct taaactgccc
    39781 tgcatcaatg ccatgttgct tcattttccg ccacagcgta gtgcggccga tcccaagtaa
    39841 agccgacatt tcctgaatgc gaccgcctgt gacctgggct gcgttaataa ttgcctcttt
    39901 ttcaacttcc gcaaatgaca gactggtgga aaggcgggtg gcgctgacat catctgtcgc
    39961 ctgctcggta aacagatgtt caggtaaatc actgacgcga atgcgcccgt tatcactact
    40021 cagagcaaga ttctcgatga cgctgtaaag ttcaaaatcg ttgcctggcc atgcacaaga
    40081 aactaggcga gcgagggcat cgtcatcaat tttcagccgc gtagagaagc gtttttcaag
    40141 actgcgtaat ttgttattca ccagcgccgg aatgctgcca cgccgcatac gcagaggcgg
    40201 gatggtaatt tcaaatgcat gcagcgcgta atacagctgg cgactaaaac gattttgttc
    40261 caccagcatt gcgaggtccg cggtcgttgt tgcaatcact ttgacatcaa ttggtattaa
    40321 acgccgcgca tccagtcgcg tgataacccc ctgcttgata acctgcagca aagcagactg
    40381 taactccacc gccagatatt caatcttttc aagaaacagc gtgccgccgt gtgccagttc
    40441 cagccgactc agacggccat tttcattgtc cgtgcgatcg ccaccaataa attcttccgc
    40501 cagcgcagca tcaccatata actcacaatt gacggcgata taaggacctg cagcacgctc
    40561 gctttcatta tgaattgcct gacttagcag tgccttgccc acgccctctt ctccacaaag
    40621 caggacagga aagctactgc gcgccgcctg gcgaccaaaa tgaatcaagc ggcgggtttg
    40681 cggatcgtcc tgtggcatat gagcgaaggt atggctgact tttcctaatt gactggtcat
    40741 taactgccgc atctgttcca caggatggag caacaaaata aagctggttc cctgcgtttc
    40801 tattatcggt ttaagggtta tcaccgcatc aataaactgg tgctggcttt caaaggttgc
    40861 ttctacgtgt ttgagcggat gtgccagttt tattgcttgt tgcaatacgg cgggtaacgt
    40921 taagagttca gtgattgccc gtccctgact tgccgtcgcg tcaaggcgca agacccgcgc
    40981 cgcctgggca ttaataaatt gcaaattacc ctgctcgtcc cagctaatca cgccatcatc
    41041 catactttct aacagggcat taagttgatt taaatgacgg ttagtttcag cgagcaaact
    41101 gtccgtcagc agtaaattac cgacctcgcg ggcgattgcc aacgtcaacg gcaaatcagc
    41161 tgcggtagtt tgctcaaccg gacacgccag cgctattgtt cccgtcaatc ggcccttgct
    41221 gtcaaacaac ggcgttgcac aaaaggccca gttccagagt gcctgtttga aatgttgatc
    41281 ggccatcgtt ttcacggcct gaccagagat agccgctaac gatagcgcac aagtaccaat
    41341 aattccctcg gcgcaatacg tgccgtcatt gaatcccagt gcacttagct gctgcaaggt
    41401 ttgcggatcg ccattacggc tgagaatgca ggcggtttca tcgaggataa acagcgcaca
    41461 ctctcgcggt gccatatatt cccaggcatc ttccagcgca gcctgcccga gcgtcagcat
    41521 cgctttctta cgccgataaa tagaagcaaa tgtcacgccc tgggcctgat gtggtacgtt
    41581 ccatgtctcc cgtttcatca gcttattgca tcgctcccag gaggttgcaa ttaagggaga
    41641 tatgccccga ccatcgttgt taaaagcgcc actcatatcc cgcatccctt atgacaaaag
    41701 gtgttccggc tcgcaatttt aagtattaat acacgtaaaa gtttcaatat ggaacattca
    41761 ttaaagcata tgtttcacta tgaaacataa tttgggctaa ttttcttttc ccttgcctga
    41821 tgcacaatgg attcatcggc agtcagtggt cgccgtgtcg ttgaacatca tccatgccct
    41881 accgtaattg ctggagcaaa ataatgaaaa aattgatcaa tgatgtgcaa gacgtactgg
    41941 acgaacaact ggcaggactg gcgaaagcgc atccatcgct gacactgcat caggatccgg
    42001 tgtatgtcac ccgagctgat gcccctgttg caggaaaagt cgccctgctg tcgggtggcg
    42061 gcagcggaca cgagccgatg cactgtggtt atatcggtca ggggatgctt tcgggggcct
    42121 gtccgggcga aattttcacc tcaccgacgc ccgataaaat ctttgaatgc tccatgcaaa
    42181 ttgatggcgg cgaaggtgta ctgttgatta tcaaaaatta caccggcgat attcttaact
    42241 ttgaaacagc gaccgagtta ctgcacgata gcggcgtaaa agtgaccact gtggtcattg
    42301 atgacgacgt tgcggtaaaa gacagtcttt ataccgccgg gcgacgcggc gttgccaaca
    42361 ccgtattaat tgaaaaactc gtaggcgcag cggcggagcg tggcgactca ctggacgcct
    42421 gtgcggaact ggggcgtaag ttgaataatc aaggccactc aataggtatc gctctcggtg
    42481 cctgtaccgt tcctgccgcg ggcaaacctt cttttaccct tgcggataat gagatggagt
    42541 ttggcgtagg cattcatggt gagccaggta ttgaccgccg ccctttctct tcccttgatc
    42601 aaaccgtcga tgaaatgttc gacaccctgc tggaacatgg ctcataccat cgcactttgc
    42661 gtttctggga ttatcaacaa ggcagctggc aggaagaacc acaaaccaaa caaccgctcc
    42721 agtctggcga tcgggtgatt gcactggtta acaatcttgg cgcaactccg ctttctgagc
    42781 tgtacggcgt ctataaccgc ctgaccacac gttgccagca agcgggattg actatcgaac
    42841 gtaatttaat tggcgcgtac tgcacctcac tggatatgac cggtttctca atcaccttac
    42901 tgaaagttga tgacgaaacg ctggctctct gggacgcccc ggtccacacc ccggccctta
    42961 actggggtaa ataaggagaa agcaatgtca ctgagcagaa ctcaaattgt taactggctc
    43021 actcgttgtg gcgatatttt caacaccgag agcgagtatc ttaccgggct ggatcgcgag
    43081 attggcgacg ctgaccacgg gctaaatatg aaccgaggct ttagcaaagt ggtggaaaaa
    43141 ctccctgcta tcgcagataa agatatcggt ttcattctca aaaataccgg tatgacgctg
    43201 ctttccagcg tcggtggtgc cagtggtccg ctgttcggta ccttctttat ccgcgccgca
    43261 caggcgaccc aggcacggca aagcctgaca ctggaagagc tttatcagat gttccgcgat
    43321 ggcgcggacg gcgtaatcag tcgcgggaaa gccgaacctg gcgataaaac catgtgtgat
    43381 gtgtgggtgc cggtggtgga atcgttacgt cagtccagcg agcaaaatct ctctgttccg
    43441 gtggcgctcg aagctgccag tagcatcgcc gaatccgctg cacaaagtac gattacgatg
    43501 caagcccgca aaggccgcgc cagttatctc ggtgaacgca gtattggtca ccaggatccc
    43561 ggcgcgacct cggtgatgtt tatgatgcaa atgttggcgt tagccgcaaa agagtaagga
    43621 attggtgatg gtaaacctgg tcatagtttc acatagcagc cgactgggag aaggtgtcgg
    43681 tgaattagcc cgtcagatgt taatgagtga tagttgtaaa atcgccattg ccgcgggaat
    43741 tgacgatcca caaaatccca ttggtaccga tgccgtcaaa gtgatggagg ccatcgaatc
    43801 tgttgctgat gccgaccatg tgctggtcat gatggatatg ggtagcgcat tattgagtgc
    43861 tgaaactgcg ctggaattgc tggctcccga gatcgccgca aaagtacgtt tgtgtgctgc
    43921 gccgttggtc gaaggtacac tggcagcaac ggtcagcgcg gcctcggggg cggatatcga
    43981 caaagttatc tttgacgcta tgcatgcgct ggaagccaaa cgtgaacaac tgggtttacc
    44041 gtcctccgac actgaaatct ctgacacatg tcctccgtac gatgaagaag cccgttctct
    44101 ggcggtggtc ataaaaaacc gtaacggcct gcatgtacgt ccggcctccc ggctggttta
    44161 taccttatcg acatttaatg ccgatatgtt gctggaaaaa aacggcaaat gcgtcacacc
    44221 agagagtatt aaccagattg cgttactaca agttcgctat aacgatacgc tgcgcctgat
    44281 tgcgaaaggg ccagaagctg aagaggcact gatcgctttc cgtcacctgg ctgaagataa
    44341 ctttggtgaa acggaggaag tcgctccacc tactctgcgt cccgttctgc ctgtttcggg
    44401 taaagccttt tattatcaac cagttttatg tacggtacag gcaaaatcaa ccctgaccgc
    44461 ggaagaagaa caagatcgat tacgccaggc tattgacttc acgttattag atctgatgac
    44521 gttaacagcg aaagcagaag ccagcgggct tgacgatatt gccgcaatct tttctggtca
    44581 ccatacactg ttaggtgatc cggaactgct ggcggcggca agcgaactcc ttcagcatga
    44641 acattgcacg gcagaatatg cctggcagca agttcttaaa gaacttagcc agcaatacca
    44701 gcaactggat gatgaatatc tacaagctcg ctatattgat gtggacgatc ttctgcatcg
    44761 caccctggtc cacctgaccc aaacgaaaga agaactcccg cagtttaact cgccaactat
    44821 tctactggcg gagaacattt atccttccac agtactgcaa ctggatccgg cggttgtaaa
    44881 aggtatctgc cttagcgccg gaagtccggt atcccacagc gccctaatcg cccgtgaact
    44941 ggggattggc tggatttgcc agcagggtga gaaactgtat gcgatacaac cagaagaaac
    45001 gctaacgctg gacgttaaaa cgcaacgttt caaccgtcag ggttaagcca ccgttcggat
    45061 ggcatcgttc tgatgtcatc cggcaattta cccagcactg atgtttttta actacctgtt
    45121 atatttttta ccaatttatg atgatttttc ttcgctaatc gcgaaccaaa tgtcgctggc
    45181 tggctaataa acagacgtta tttgatcact ctatttcgcc gttatttatc cctttaattc
    45241 ccttttctaa aatgcctgac cgttcgcaga atgagatttc gatcatgcag ctagtgcgat
    45301 cctgaactaa ggttttctga tacttgaata ccgttttatt cggtttcgcc aaaggagaat
    45361 gattgatgaa atcccccgca ccttctcgcc cgcaaaaaat ggcgttaatt ccagcctgta
    45421 tctttttgtg tttcgctgcg ctatcggtgc aggcagaaga aacaccggta acaccacagc
    45481 cgcctgatat tttattaggg ccgctgttta atgatgtgca aaacgtcaaa ctttttccgg
    45541 accaaaaaac ctttgccgat gccgtgccta acagcgatcc gctgatgatc cttgctgatt
    45601 atcggatgca gcaaaaccag agcggatttg atctgcgcca tttcgttaac gtcaatttca
    45661 ccctgccgaa agaaggcgag aaatatgttc cgccagaggg gcagtcactg cgcgaacata
    45721 ttgacggact ttggccggta ttaacgcgtt ctaccgaaaa caccgaaaaa tgggattctc
    45781 tgttaccgct gccggaacct tatgtcgtgc cgggcggacg ttttcgcgag gtatattact
    45841 gggacaatta cttcaccatg ttaggacttg ccgaaagtgg tcactgggat aaaatcgcgg
    45901 atatggtggc caattttgct catgaaataa acacttacgg tcatattccc aacggcaacc
    45961 gcagttacta tttaagccgc tcgcaaccgc ccttctttgc cctgatggta gagttactgg
    46021 cgcagcatga aggcgatgcc gcgttgaagc aatacctgcc gcaaatgcaa aaagaatatg
    46081 cttactggat ggacggtgtt gaaaacctgc aagccggaca acaggaaaaa cgcgttgtaa
    46141 aacttcagga tagtaccctt ctcaaccgct actgggacga tcgcgatacg ccacgaccag
    46201 agtcatgggt ggaagatatt gccaccgcca aaagcaatcc gaatcgacct gccactgaaa
    46261 tttaccgcga cctgcgctct gccgctgcgt ctggctggga tttcagctcg cgctggatgg
    46321 acaacccgca gcagttaaat accttacgca ccaccagcat cgtaccggtc gatctgaaca
    46381 gcctgatgtt taaaatggaa aaaatcctcg cccgcgccag caaagctgca ggagataacg
    46441 cgatggcaaa ccagtacgaa acgctggcga atgcccgtca aaaagggatc gaaaaatacc
    46501 tgtggaacga tcaacaaggc tggtatgccg attacgacct gaaaagtcat aaagtgcgca
    46561 atcagttaac cgcggccgcc ctgttcccgc tgtacgtcaa tgcggcagcg aaagatcgcg
    46621 ccaacaaaat ggcgacggcg acgaaaacac atctgctgca acccggcggc ctgaacacca
    46681 cgtcggtgaa aagtgggcaa caatgggatg cgccaaatgg ctgggcaccg ttacagtggg
    46741 tcgcgacaga aggattacaa aactacgggc aaaaagaggt ggcgatggac attagctggc
    46801 acttcctgac caatgttcag cacacctatg accgggagaa aaagctggtg gaaaaatatg
    46861 atgtcagcac caccggaacg gggggcggcg gtggcgaata tccattacag gatggctttg
    46921 gctggaccaa tggcgtgacg ctgaaaatgc tggatttgat ctgcccgaaa gagcaaccgt
    46981 gtgacaatgt tccggcgacg catccgaccg ttaagtcagc aacgacgcaa ccctcaacca
    47041 aagaggcaca acccacacct taaccagcgc ttactccgac aacgtcattc ggcttctccg
    47101 gatggcgttg tcggcagata acaccatatc tatggttttt tctacacaga cggtttttca
    47161 agtcctggcg ggaagtattg cgcaaatgtt tgccatacgt ggttgccgaa gatagcgtca
    47221 tttctacggg taagtgtttc actttcatca cccggcgatt tacgccgacg ttggctatat
    47281 aaagcatcaa ttgctaccga atattgttac ggtgttcact catcaacgtt tttttgcttt
    47341 ctggagttaa attaataatc acataatcca gacactccag agaatttagc aaccaaaaaa
    47401 agaattcaga ccattcctct tcgcgtaact gaagcattgc cgaacgggta ttttggctta
    47461 atctgccaga aggattgatc aattctaaat gttcaataag cgcagccaaa accgcctcac
    47521 tatccgcccc atttttaacg cctgactggc tactgtctaa aatatttatt ttctgaatca
    47581 caccaaaaac aacgaaacaa taattaaaaa aggattctgt tgttgttacc gggaaccgac
    47641 ttatttcgct cagaaagtcg ttcaaccagc tccgcagtga taagattcgc tgatgataca
    47701 gtgtatcctc actgcgtgct ggaggtgcca gtataatatg ttgatcaata cagtatttat
    47761 tgtgttcagt cgtttcctga tgcgtttggt tctctgcgga ttgtcgctgg cgaaataaat
    47821 ggcttgcggt aaatgccgtt acagagcagc gcaacgcgtc aattccttcg gagtgcccga
    47881 cttttggcga gagttccggt tttatgcgtc caggtagcgg aaatttcacc cgaatgagaa
    47941 atgcattaat aaaatg
//
LOCUS       DAJTSA010000023        47508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000023 DAJTSA010000000
VERSION     DAJTSA010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.023"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            172..654
                     /gene="sseA"
                     /locus_tag="OO369_001368"
                     /pseudo
     CDS             172..654
                     /gene="sseA"
                     /locus_tag="OO369_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
     gene            complement(1473..2249)
                     /gene="sseB"
                     /locus_tag="OO369_001369"
     CDS             complement(1473..2249)
                     /gene="sseB"
                     /locus_tag="OO369_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR5981700.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2391..3674)
                     /gene="pepB"
                     /locus_tag="OO369_001370"
     CDS             complement(2391..3674)
                     /gene="pepB"
                     /locus_tag="OO369_001370"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR5981701.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3733..3933)
                     /gene="iscX"
                     /locus_tag="OO369_001371"
     CDS             complement(3733..3933)
                     /gene="iscX"
                     /locus_tag="OO369_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR5981702.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3945..4280)
                     /gene="fdx"
                     /locus_tag="OO369_001372"
     CDS             complement(3945..4280)
                     /gene="fdx"
                     /locus_tag="OO369_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR5981703.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4282..6132)
                     /gene="hscA"
                     /locus_tag="OO369_001373"
     CDS             complement(4282..6132)
                     /gene="hscA"
                     /locus_tag="OO369_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR5981704.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(6149..6664)
                     /gene="hscB"
                     /locus_tag="OO369_001374"
     CDS             complement(6149..6664)
                     /gene="hscB"
                     /locus_tag="OO369_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR5981705.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6760..7083)
                     /gene="iscA"
                     /locus_tag="OO369_001375"
     CDS             complement(6760..7083)
                     /gene="iscA"
                     /locus_tag="OO369_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR5981706.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(7100..7486)
                     /gene="iscU"
                     /locus_tag="OO369_001376"
     CDS             complement(7100..7486)
                     /gene="iscU"
                     /locus_tag="OO369_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR5981707.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7514..8728)
                     /gene="iscS"
                     /locus_tag="OO369_001377"
     CDS             complement(7514..8728)
                     /gene="iscS"
                     /locus_tag="OO369_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR5981708.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8840..9328)
                     /gene="iscR"
                     /locus_tag="OO369_001378"
     CDS             complement(8840..9328)
                     /gene="iscR"
                     /locus_tag="OO369_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR5981709.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9598..10338)
                     /gene="trmJ"
                     /locus_tag="OO369_001379"
     CDS             complement(9598..10338)
                     /gene="trmJ"
                     /locus_tag="OO369_001379"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR5981710.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10457..11260
                     /gene="suhB"
                     /locus_tag="OO369_001380"
     CDS             10457..11260
                     /gene="suhB"
                     /locus_tag="OO369_001380"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR5981711.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11405..12259
                     /locus_tag="OO369_001381"
     CDS             11405..12259
                     /locus_tag="OO369_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5981712.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     APWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12450..13730
                     /gene="csiE"
                     /locus_tag="OO369_001382"
     CDS             12450..13730
                     /gene="csiE"
                     /locus_tag="OO369_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR5981713.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13722..14861)
                     /locus_tag="OO369_001383"
     CDS             complement(13722..14861)
                     /locus_tag="OO369_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR5981714.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(15021..15911)
                     /gene="hcaR"
                     /locus_tag="OO369_001384"
     CDS             complement(15021..15911)
                     /gene="hcaR"
                     /locus_tag="OO369_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR5981715.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            16047..17408
                     /locus_tag="OO369_001385"
     CDS             16047..17408
                     /locus_tag="OO369_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR5981716.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRRADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17405..17923
                     /locus_tag="OO369_001386"
     CDS             17405..17923
                     /locus_tag="OO369_001386"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR5981717.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17923..18243
                     /locus_tag="OO369_001387"
     CDS             17923..18243
                     /locus_tag="OO369_001387"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR5981718.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18240..19052
                     /gene="hcaB"
                     /locus_tag="OO369_001388"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18240..19052
                     /gene="hcaB"
                     /locus_tag="OO369_001388"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR5981719.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            19062..20279
                     /locus_tag="OO369_001389"
     CDS             19062..20279
                     /locus_tag="OO369_001389"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCR5981720.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRSAAQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVV
                     DGEKVELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIF
                     AGGDVAITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQ
                     FIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDA
                     KLLIDENIALKSL"
     gene            20376..20798
                     /locus_tag="OO369_001390"
     CDS             20376..20798
                     /locus_tag="OO369_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5981721.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20846..21718)
                     /locus_tag="OO369_001391"
     CDS             complement(20846..21718)
                     /locus_tag="OO369_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR5981722.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21730..22824)
                     /locus_tag="OO369_001392"
     CDS             complement(21730..22824)
                     /locus_tag="OO369_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5981723.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22857..23855)
                     /locus_tag="OO369_001393"
     CDS             complement(22857..23855)
                     /locus_tag="OO369_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5981724.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23880..25391)
                     /locus_tag="OO369_001394"
     CDS             complement(23880..25391)
                     /locus_tag="OO369_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5981725.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25414..26396)
                     /locus_tag="OO369_001395"
                     /pseudo
     CDS             complement(25414..26396)
                     /locus_tag="OO369_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(26493..29594)
                     /locus_tag="OO369_001396"
     CDS             complement(26493..29594)
                     /locus_tag="OO369_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR5981726.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISVGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWVNPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKKPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29601..29774)
                     /locus_tag="OO369_001397"
     CDS             complement(29601..29774)
                     /locus_tag="OO369_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981727.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29892..31085
                     /locus_tag="OO369_001398"
     CDS             29892..31085
                     /locus_tag="OO369_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR5981728.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31283..32536)
                     /gene="glyA"
                     /locus_tag="OO369_001399"
     CDS             complement(31283..32536)
                     /gene="glyA"
                     /locus_tag="OO369_001399"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR5981729.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32863..34053
                     /gene="hmpA"
                     /locus_tag="OO369_001400"
     CDS             32863..34053
                     /gene="hmpA"
                     /locus_tag="OO369_001400"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR5981730.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(34098..34436)
                     /gene="glnB"
                     /locus_tag="OO369_001401"
     CDS             complement(34098..34436)
                     /gene="glnB"
                     /locus_tag="OO369_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR5981731.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34497..35831)
                     /gene="glrR"
                     /locus_tag="OO369_001402"
     CDS             complement(34497..35831)
                     /gene="glrR"
                     /locus_tag="OO369_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR5981732.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35821..36534)
                     /gene="qseG"
                     /locus_tag="OO369_001403"
     CDS             complement(35821..36534)
                     /gene="qseG"
                     /locus_tag="OO369_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR5981733.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36699..38126)
                     /gene="qseE"
                     /locus_tag="OO369_001404"
     CDS             complement(36699..38126)
                     /gene="qseE"
                     /locus_tag="OO369_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR5981734.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38684..42571)
                     /gene="purL"
                     /locus_tag="OO369_001405"
                     /gene_synonym="purI"
     CDS             complement(38684..42571)
                     /gene="purL"
                     /locus_tag="OO369_001405"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR5981735.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42830..44386
                     /gene="mltF"
                     /locus_tag="OO369_001406"
     CDS             42830..44386
                     /gene="mltF"
                     /locus_tag="OO369_001406"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR5981736.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44383..44919)
                     /gene="tadA"
                     /locus_tag="OO369_001407"
     CDS             complement(44383..44919)
                     /gene="tadA"
                     /locus_tag="OO369_001407"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR5981737.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44944..45579)
                     /gene="yfhb"
                     /locus_tag="OO369_001408"
     CDS             complement(44944..45579)
                     /gene="yfhb"
                     /locus_tag="OO369_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR5981738.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45788..46636
                     /locus_tag="OO369_001409"
     CDS             45788..46636
                     /locus_tag="OO369_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR5981739.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46975..47508)
                     /locus_tag="OO369_001410"
                     /pseudo
     CDS             complement(46975..47508)
                     /locus_tag="OO369_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11327 a        13021 c        11952 g        11208 t
ORIGIN      
        1 acgttacaga cggtcccggc aaaagtgcct gacctgaccc ctattagcgg ttaccagcgc
       61 cccaccagac ccggtgacat ccccccccgg atcatggacg actgggggca gtaatcatgc
      121 cgggtctggc tggctaacac cccattataa ggggtacgaa taaagtaacg gggacttgca
      181 ggctggcagc gcgatgacct gctattagaa gaaggtgcag tagagctgcc ggaaggagag
      241 tttaacgccg cgtttaatcc tgaagcagtg gtgaaagtaa ccgatgtgtt attggcaagc
      301 catgaaaata cggcgcaaat tattgatgcc cgcccggctg cacgttttaa cgcagaagtt
      361 gatgaacctc gcccaggttt acgtcgcgga catattcccg gtgcattgaa cgttccgtgg
      421 acggaactgg tgcgcgaagg cgaactaaaa acgaccgatg aactggatgc gatatttttt
      481 ggtcgcggcg tcagctatga caaaccgatt atcgtcagct gcggctctgg tgtaacggca
      541 gccgtggttt tgttagcact cgcgacgctg gatgtgccaa acgtgaaact gtacgacggc
      601 gcatggagtg aatggggcgc gcgggcagat ttaccggttg agccagtgaa ataagtatat
      661 tacaggcaat aaaaaaccgc cgaatttggc gtttttttat tgctggtctg gttcgcggcc
      721 tttccagcag gttgacttgt gttacatgag caacgcaggt gcttcacagc aaaacaatac
      781 tcaccagtaa ctctcttttt gtcaagcaaa agagagtaat tattgtttat ttagcgtatt
      841 atcgacaccg gccctttccg ccgtgttcgg taataaaata acctggctta ttagtccgaa
      901 ttcagacaaa tataaacaaa tcctgctcaa aattaaaaat tctaaccggt aaaagatatt
      961 acttaaacat gtaaatttac tttcctttaa aaaacaaaaa accgccaaaa tcaggcggtt
     1021 atttgttgct ggtccggttc gcggcctttc cagcaggttg tattaccgta gtaatgcaag
     1081 cgcgtctcag cggagacaat actcgccagt aactctcttt ttgtcaagca aaagagagtt
     1141 attattgttc tgttagtgta ttatccactg cggccctttc cgccgtctcg caagcgggcg
     1201 ctggcttaag gaaaggatgt tccgcgaccg ttagagcaag cgcgtctcag cgcttgctat
     1261 cgcggcaata tcgccagtgg tgctgtcgtg atgcggtctt cgcatggacc gcacaatgaa
     1321 gatacggtgc ttctgtatcg tacttattgt ttctggtgcg ctgttttccc aagtaaataa
     1381 taaccggagt ctctccggcg acaatttact ggtggttaac aaccttcaga gcagcaagta
     1441 agcccgaatg ccgcccttta gggcggcata ttttagatta tccggttttg tttaaagtca
     1501 cgcaaaaaac caccccagcg acgttcatag aatggcgcaa tatgttcggt aataaagtgg
     1561 ctaattcctt tttccccttt tttcacctga caaatatcga ttggttcatc gccaggtaat
     1621 gtatcggtcg ctacacttcc cgtcgcctga ataatttctt cgatatcacc atcggcttca
     1681 atgccaataa gtaaattagg ctgtgcctct tcgttctctt taattgaaca aataaaagca
     1741 cgcttcaccg gcttaatggt tttaaataag gtggtgagtg aatcaatcat ttgtgctggc
     1801 ggctctgcga cttccgataa tatcagcgat tcaccgcctt ccaggatttc ctggctgctc
     1861 agcggatttc cctcttcacc aatcaacaaa ctgatttcac gcggcataaa ttctttaccg
     1921 gtcggcagtt tggcattaag gaagagcgtt tcgccaaggg tcatctcaaa caatgtgcga
     1981 acgggcatta cgacaaatgc ctgttcgtct tcaaccgcct gttgaagcgc ttctaacgag
     2041 gtgaaaaaag gaatgacgct ggtgccgtct tctttttccc agtgctgtaa atcaagcgca
     2101 ctatcttcaa ccacagcctc gccctgcgcc gccgtaccag gcacccagac ggtggattcc
     2161 agtagagtac ggaaaaaggc cgggcggtgc gccggttcgg ttgctgcttt ttccagcagg
     2221 tcttcaagtt cgttttttgt ttcggacata agaaccacaa ttcattcaac gttcggggcg
     2281 caaatgcctg atgcgctacg cttatcaggc ctacaaggga ttcgcaattt gttgaatttg
     2341 cagaatttgt aggccggata aggcgtttac gccgcatccg gcatattagt ttacgccgtt
     2401 aacagattag ctatcgtgcg cacaccaagt cccgtagcgc ccgcagacca ctgttcaacc
     2461 ggcgctttac ggtaagtcgc cgagcagtcg atatgcagcc agccttgctg atagttctca
     2521 acaaagtgcg acaggaagcc cgccgccgtg ctcgcgcctg ccggatacgc cgcgcttccg
     2581 gtattgttca gttcggcaaa gttagacggc agctggctgc ggtggaattc cgccagcggc
     2641 agacgccaga atggttcgtt ctcttgtgag gcactcgcca gtaaacgacc ggcaagcgca
     2701 tcgtcaaaac tgaacagcgc gtgataatca ttacccagcg cagttttcgc cgccccggtg
     2761 agggtcgccg catcaatgat catttccggt ttctgcgcac tggcatcaat cagaccatcg
     2821 gcaagcacca gacgcccttc ggcatcagtg ttcatcactt caactttttt accgttgcga
     2881 taggtgatga tatcgcccag cttgaacgca ttgccgctaa tcaggttatc cgcacagcag
     2941 aggaacagct tcacgcgctt gttcagtccg cgcataatgg caaatgccag cgccccggta
     3001 accgttgccg cgccgcccat gtccgacttc atcgagtcca taaacgcagt ctgtttgatg
     3061 ctgtagccgc cggagtcaaa agtgatacct ttacctacca ggcacgcgta caccggtgct
     3121 tctttatcgc cagttgggtt gtaatccagc gccagcaata ccggagaacg ttctgaaccg
     3181 cgcccgacag tgtgcagccc catataacct tgctcacgca gatcttcgcc tttggtgatc
     3241 cgataagtca cacgatcgcc cgcgacgttg ctgatcagat caacagcacg ctgtgccagt
     3301 tgcgatggtc ccaattcttc tgccggtgcg ttgatggtgt cacgcaccca gtcgatgatc
     3361 atcaggcggt tatccagttc ctggcgctgg gcatcgtcca gatccggcca ctccacttta
     3421 cgcgtgcctt tcgggccttt ataaccttgc cagaatgccc agcagcgatc cgcatcccag
     3481 ccttcaccgc ttaactgaac atgcttgatg cccagaccgt caatcttgcg ggccgcacgc
     3541 tggatcagcc ccagatcgtc tgccccgttc aaatgcaggg taatgccgtc attattaatg
     3601 ctgtaagttg ctttttctcc ccagcgcgcg tcggcaggtt gggtagagag ggtaatcttc
     3661 atcgcttctg tcattttagt tatccttatt cgtttaacag caaaacgggc tacccccagg
     3721 cagcccgttc attcactcgg cttcgtccag ccagactaac aaaatcgctt cgaggatttt
     3781 ctcgttggat gcctgcgggt cgtcgtcgaa atcttccaga tcgcaaatcc actgatgcat
     3841 atcggtgaat cgaaccgttt tcggatcaag atcgggatac gcatcgtaca gtgcttcgcc
     3901 aatttcgcgg ctatcggtcc acttaagtcc catactaacc tctgttaatg ctcacgcgca
     3961 tggttgatag tgtaacgcgg gatttcgacc actaaatctt cgtcggtgac gcgcgcctgg
     4021 cagcttaaac ggctttccgg ctccagtccc caggctttgt ccagcatgtc gtcttcctgc
     4081 tctgagcttt ccggcagtga gtcaaaacct tcacgaacga tgcagtggca ggtggtgcaa
     4141 gcacaggatt tttcacaggc gtgttcaatc tcaataccgt tacgcagcgc tgcgtcgaga
     4201 atggtttcac cgctattagc ttccagaaca gcgccatcag ggcagaggtc ctgatgaggc
     4261 aaaataacaa tctttggcat attaaacctc gtccacggaa tggcctttca gcgcacgacg
     4321 aaccgactgg tccatgcggc gagcggcgaa atcctgggtt tgtttgtcta cgtttttaat
     4381 cgcttgttcg atggcgtcaa catcatcgcc ctgcgccact tcactcaggt gagcggcagc
     4441 attgtcaatg acctgacgct ctgcggcgct taacagcgcg gcatcagcag ccagcgcgcc
     4501 gtgcagactt tccagcacac gcgccgcttc tactttttgt tctgccagca tacgggcttt
     4561 tacgtcctgc tcggcatagc tcattgagtc tttgatcatc gaagcgattt cgctatcggt
     4621 cagaccgtaa gacggtttga cctgaataga cgcctcaacg ccggtggatt tctccatcgc
     4681 cgtcacgctc aaaagaccgt cggcatcgac ctggaacgtc acgcgaatat gcgcaccgcc
     4741 agccggtagc gccggaatac cacgcagcgc aaaacgcgcc agtgagcggc agtcctgcac
     4801 cagttcgcgc tcaccctgca ttacatggat agacatcgcc gtctgaccat ctttaaaggt
     4861 ggtgaaatcc tgagcgcggg ccaccggaat agtggtatta cgcggaatca ctttctccac
     4921 caggccgccc atcgtttcga ggcccagcga cagtgggatc acatcaagca gcagcatttc
     4981 gctgtctggc ttgttaccca ccagaatatc cgcctgaatc gccgcgccaa tagcgacgac
     5041 tttatccggg tcgatggaag tcagcggtgg acgaccgaaa aattcgccta cccgttcacg
     5101 caccagcggc acgcgagtag aaccgcccac catcaccact tccagcactt cgtcagcttc
     5161 tacacccgcg tctttcagcg cgcgacgaca agccagtaag gttcgtttta ccagtggcgc
     5221 gatcagttca ttgaattgtt cacggctgat ttcgccctgc cagcccgcaa cgttaacggt
     5281 cacggagtcc gcatcgctca gcgcgatttt ggctgcaatg gtggcatcca gcagttcacg
     5341 ctgaacgcgg ttatcgctac gatcaggaat gtccgcctgc tcgcgaatgt aatccgccag
     5401 cagatggtcg aaatcatcgc cgccgagcgc ggaatcaccg ccggttgcca gcacttcaaa
     5461 cacgccgcga cttaagcgca gaatggaaat atcaaacgtc ccgccaccga ggtcataaac
     5521 agcgatcacg ccttcctgac cggaatccag cccgtaggcg atagccgcag cagtcggttc
     5581 gttaagtaag cgcagaacat gcaaccccgc cagacgcgcc gcgtctttgg tgccctgacg
     5641 ctgggcatcg tcaaagtacg ccggaacggt gataactaca ccatccagct cgcctgccag
     5701 ggcttcagtt gcccgcgccg ccagtgcttt gaggatgtcc gcagaaacgc gcaccgggtt
     5761 cagtagcccc gccgccgttt caatcatcgg caggccgttt tcgctggcct ggaattgata
     5821 aggcagatgc ggatagcgtt gctggatatc agccagcgag cgccccatca ggcgtttaac
     5881 agaactaatg gtgttggcgg tgtcaagcgc tgcgttagta cgcgcgtcat aacccaccga
     5941 atgcccttgc tgttgatagt gaacaacaga tggcagcagg tgacggcctt catggtcggc
     6001 taacgtttcg gcctgaccgc tgcgcactgt cgccaccagc gagttggttg tgcccaggtc
     6061 aataccagcc gccagacgac gctgatgcgg cgcagcactc aaaccaggtt cactaatttg
     6121 taataaggcc atgtttagct tccagaaatt aaaaatcgag cagtttttct tcgagttgtt
     6181 cggcactgct ccgcagttta tcgagaaaac gcagcttacg caccgtatcc gccgccgcgt
     6241 cccacgcctc gttgtctaac tgttcaacca tcaactgatg gcgggtatca aacatctttt
     6301 tcacacgttt gataaagctt tccagccgcg cttcatcttt cgcctgttcg atctcgtcca
     6361 gctcttcgcg cagctccaac tgttccatca ggaacgcggt gtcgcgcaca gtatgctgct
     6421 cgctggcgag atcaaaaccg tgtaaagaaa gcaaatattc cgcgcgcatt aacggatgac
     6481 gcagcgtttg ccaggcctgg ttaatggttg cagattgctg tacggcggcg agttgttccg
     6541 ccaggcttcc gctggcgaat ttatcaggat gatactgacg ttgtagatcc tgaaaacgca
     6601 ggctcagtgc ctgggtatcg agttgataac gggcaggcaa gccaaagagg gtgaagtaat
     6661 ccataacatt ctcagggctg cgatccgccg cgataacggc gaatcaggta aaacaaaccc
     6721 cacgcgcagg cgaccacggt ggggttatcg gtatgcgcat caaacgtgga agctttcgcc
     6781 gcaaccacac tcatctttaa cgttcgggtt ggtgaattta aacccttcat tcaggccttc
     6841 ttttacgaag tccagctgcg taccgtccag aaattgcagg cttttgccat cgaccacgac
     6901 tttcacgcct ttgtcttcaa acacgatgtc ttccggcgtc ggttcgtcaa caaattccag
     6961 tacataagcc atacctgaac atccggaggt tctcacgccc agacgcaggc caaacccttt
     7021 gccgcggtta gccagaaagg tatttactcg cgctgctgca ctgtcgctca gtgtaatcga
     7081 cataaccaaa cctcaactct tattttgctt cacgtttgct tttatagtcc gcaatggcgg
     7141 ctttgatcgc gtcttctgcc agaatagaac agtgaatttt caccggcggc agttcaagtt
     7201 cttcagcaat atcggtgttt ttgatcgcct gcgcttcgtc gagagacttc cctttcaccc
     7261 attcggtgac cagggagctg gaagcgatag cggaaccgca gccgtaagtt ttaaaacgcg
     7321 cgtcttcaat gataccttca tcgttgactt taatctgcaa cttcattacg tcgccacagg
     7381 ccggtgcacc caccatgccg ctgccgacgt tctcgtcgtt gttatcaaag gaacccacgt
     7441 tacgcggatt ctcgtaatgg tcgataactt tttcgctgta agccattata aattctcctg
     7501 attccgatac cgattaatga tgagcccatt cgatgctgtt cagatccacg ccctgcttgt
     7561 acatttccca cagcggagaa aggtcacgca gacgaccgat ggatttacga actaactcga
     7621 tggtgtagtc gatctcttct tcagtagtaa aacgacctaa agagaaacgg atagagctat
     7681 gtgccagctc gtcgttcagc cccagcgcgc gcagcacgta ggacggttcg aggcttgctg
     7741 acgtacaggc ggaacctgaa gaaactgcga ggtctttcag cgccataatc agcgactcac
     7801 cttcaacgta gttgaagctg acgttgagaa tgttcggcgc accgtgttcc aggtcaccgt
     7861 tcaggtaaac ttcttcgata tctttgatgc cgttccacag acggttacgc aggccgcgca
     7921 gacgttccat ctcggtcgcc atctcttctt ttgcgatgcg ataggcttcg cccattccga
     7981 cgatctggtg aacaggcaga gtgccggaac gcataccgcg ctcgtgaccg ccgccgtgca
     8041 tttgcgcttc gatgcgtaca cgcggtttac gacgcacata cagcgcaccg atacctttcg
     8101 ggccatagat tttgtgaccg gagaaagaca tcaggtcaac tttcaactgg ctcaggtcga
     8161 ttggcagttt gcccacgctc tgggttgcat caacgtgata gataatgcca cgagcacggc
     8221 acatttcgcc gatagccgcg atatcctgca ccacgccgat ttcgttattt acgtgcatga
     8281 tggaaacaag gatggtgtcg tcacgcatcg ccgcttcgag ttctttcagg tcgataatac
     8341 cgttacgctg cggtgccagg taggtgactt cgaaaccttc gcgctccagc tgacggcaag
     8401 tatccagtac cgctttgtgt tcggttttgc tggtgatgat gtgcttgcct tttttctgat
     8461 aaaagttggc tgcacctttg atcgccaggt tgtcagattc ggttgcacca gaggtaaaga
     8521 cgatttcacg cggatcagcg ccgaccagat cggcaatctg attacgggcg atatctaccg
     8581 cttcttcagc ctgccagccg aaacggtgag aacgagaggc cgggttacca aaggttccgt
     8641 ccatcgtcat gaactgcatc attttctcgg caacacgcgg gtccaccggc gtggttgcgg
     8701 agtagtcgag ataaatcggt aatttcattg ctctataaac tccgtacatc actcaatgca
     8761 aggaatcagg ctaccggctg gatgtacgac cgtgtttacg gagtatttag cactccggcc
     8821 tgattctgaa ttctttttat taagcgcgta acttaacgtc gatcgcgtct tgtgtgcggg
     8881 tgcgtggcgc gtcgtgagta tgctgacgac cagacacatc cagcacttcc tggttattaa
     8941 ccagttcgcc taaagtaatg ttgttgagaa aaccggtgag acggtcgctc aaatcacgcc
     9001 acagcgcgtg ggtcaggcat ttatcgccgc cctggcagcc gcctttaccc tgacaacggg
     9061 tggcatctac agattcgtca acggcgctaa tcacttcgcc aacggcgatg ctgctggcat
     9121 ctttgcctaa cagataacca ccgcctggtc cacgtacgct ggaaaccaga ccatttttac
     9181 gcagacggga aaacagttgt tccagataag aaagggaaat tccctgacgt tcggaaatat
     9241 cagccaacgg taccgggccc gcttcagagt tgagcgcaac gtcaagcatt gcggtcacgg
     9301 catagcgccc tttagatgtc agtctcatgt cttacttcac ctcaaactcg cccctgcccg
     9361 gggtttttta ttgtaaaagt gggggtattg catagcaggg tcaagtctga cattcccgag
     9421 taaattggtc aactatttac ttgactgatt tagtcgggta tttaaccttc agtgccattt
     9481 ttttatcgtg gcgtttgtac ctgttgtcgg atgcggtgtg aacaccttat ccgacctacg
     9541 attctgtaca ttgtaggcct gataagacgc gttaagcgtc gcatcaggca accactgtta
     9601 ttcggccttg ttacctttat tctgctgctc aatagaagcc agaatcccgc gcaggatatt
     9661 caattcctgg ctttccgggc gcgcacgggt aaacagacgg cgcaatttat tcatcacctg
     9721 ccccggatgg ttttcacgga taaaaccggt tgccagcagc gtttgttcca gatgaccata
     9781 aaaacgctcc agatcatcga ccagcggata cggcgtctct tcatgctcga cctgctcgcc
     9841 attttcctga gttgccagcc aggccatacg cacttcatag gcaataacct gaaccgccat
     9901 tgccaggttc agcgaactgt attccgggtt tgccgcaatc gcgacatgat aatggcattt
     9961 ctgcaactct tcattggtca agccgacgcg ctcacgacca aacaccagcg ccaccggggt
    10021 atttgccgct tcagcgacgc ttttcagacc gcattcgcgc gggtcgagca tcggccacgg
    10081 cagcgtgcgg gagcgtgcgc tggtgcccac caccaggcta caaccggcta acgcttcgtc
    10141 gagcgtatcg acgatgtgag cattaccaat cacatcgctg gcccctgcag ccagggcaat
    10201 cgcctgggag tcgggtttca ccagtggatt aaccagccac agattggtta atcccattgt
    10261 tttcatggca cgggcaacag aacccatatt gccggtgtgt gacgtctcca ccagcacaat
    10321 tcgaatattt tgcagcattg tctttcttca tctaaagatt attcacgcat cttatcataa
    10381 aacgaagaca gatgccgatc tcgctgctat actctgcgcc gttttcccgt tctttaacat
    10441 ccagtgagag agaccgatgc atccgatgct gaacatcgcc gtgcgcgcag cgcgcaaggc
    10501 gggtaattta attgccaaaa actatgaaac cccggacgct gtagaagcga gccagaaagg
    10561 cagtaacgat ttcgtgacca acgtagataa agctgccgaa gcggtgatta tcgacacgat
    10621 tcgtaaatct tacccacagc acaccatcat caccgaagaa agcggtgaac ttgaaggtac
    10681 tgatcaggat gttcaatggg ttatcgatcc actggatggt actaccaact ttatcaaacg
    10741 tctgccgcac ttcgcggtat ctatcgccgt tcgtatcaaa ggccgcaccg aagttgctgt
    10801 ggtatacgat cctatgcgta acgaactgtt caccgccact cgcggtcaag gcgcacagct
    10861 gaacggctac cgtctgcgcg gtagcaccgc tcgcgatctc gacggtacca ttctggcgac
    10921 cggcttcccg ttcaaagcaa aacagtacgc cactacctac atcaacatcg tcggcaagct
    10981 gttcaacgaa tgtgcagact tccgtcgtac cggttctgcg gcgctggatc tggcttacgt
    11041 cgctgcgggt cgtgttgacg gtttctttga aatcggtctg cgtccgtggg atttcgcggc
    11101 aggcgagctg ctggttcgtg aagcgggcgg catcgtcagc gacttcaccg gtggtcataa
    11161 ctatatgctg accggtaaca tcgttgctgg taacccgcgt gttgtgaaag ccatactggc
    11221 gaacatgcgt gacgagttaa gcgacgctct gaagcattaa tgactcaggc gggtgatatc
    11281 actcacccgc cctcgccttt caggcgctat tccgaaatac ttcctcaccg ctttactttc
    11341 tttcgtcact ctcccaccat ctttccccga ttaatggatg ataaagaagt cgcaaggaaa
    11401 ggttatggca ctgccagtga acaaacgcgt tcccaaaatt ctgtttattc tctttgttgt
    11461 tgccttctgc gtttatttag tgccgcgcgt tgccatcaac ttcttctatt atcctgacga
    11521 taaaatttac ggtcccgccc cctggtcggc ggaatccgtc gaatttacgg ctaaggacgg
    11581 tactcgtctg caaggctggt ttattccttc ttcgacgggc cctgctgaca acgccatcgc
    11641 aaccatcatt catgctcacg gcaatgccgg aaatatgtcc gcccactggc cgctggtcag
    11701 ttggttacct gagcgtaatt tcaacgtttt tatgtttgat tatcgcgggt ttggtaaatc
    11761 aaaaggcact ccgtcccagg ccggattgct ggacgatacg caaagcgcca tcaatgtggt
    11821 gcgccatcgc agtgatgtaa acccacaaca gctggtgctg ttcgggcaga gtattggcgg
    11881 ggcgaatatt cttgctgtta ttggtcaggg tgatcgtgaa ggcatacgtg cggtgatcct
    11941 cgactccaca tttgcctctt atgcaaccat cgccaaccag atgatccccg gcagtggcta
    12001 cttacttgat gagagttaca gcggcgaaaa ttacatcgcc agcgtcagcc cgatcccgct
    12061 tttactcatt cacggtaaag ccgatcacgt tatcccatgg cagcacagcg aaaagttgta
    12121 tagcctggca aaagagccaa aacggttgat cctaatcccg gatggcgaac acattgatgc
    12181 tttttccgat cgtcacggca atgtttatcg cgaacagatg gtggacttta tccttagcgc
    12241 gttgaatccg cagaactaac ccatgatcgc tagcacgata atcattcaca aaaccacctt
    12301 aagacatgct aatccactgg tcagaacagt ttaaaatgag aaaaattctg tgacgcttgc
    12361 caacatttct gatgattagc attcccttcg ccatttccct gagcaaactt tagctattct
    12421 tatcaattat gcttatggga gatctacaga tgatgcctac gcttgctcca ccatctgtcc
    12481 tttcggctcc ccagcgccgc tgccagatct tgctgacgct ctttcagccg gggttgaccg
    12541 ccaccatggc aaccttcagc gagcttaatg gtgtggatga tgatattgcc agtcttgata
    12601 tcagcgaaac aggacgggag atcctgcgct atcatcaact cacactgacg actggttatg
    12661 acggtagcta ccgggttgaa ggtacagtgc ttaaccaacg tttgtgttta tttcactggc
    12721 tacgacgtgg tttccgtctg tgtccgtcat ttattaccag ccaattcacc cccgccctga
    12781 agagtgaact gaagcggcgc ggaattgcgc gtaactttta cgacgatacc aatctacaag
    12841 cgttagtgaa tctctgctcc cgacggctgc aaaaacgctt tgaatcgcgc gatattcatt
    12901 tcctgtgtct gtatctgcaa tattgtttgc tgcagcacca cgctggaatt acgcctcagt
    12961 ttaatccgct ccaacgtcgc tgggcggaat cctgccttga atttcaggta gcgcaggaaa
    13021 ttggacgcca ctggcagcgt cgggcgctcc agcatgtacc gcctgatgag ccactgttta
    13081 tggcattact tttttccatg ttgcgggttc ccgatccact gcgggatgcg catcagcggg
    13141 acagacaatt gcgtcagtct atcaaacgtc tggtaaacca ttttcgtgag ctgggaaatg
    13201 ttcgttttta tgatgaacag gggttatgtg atcagcttta tatccacctc gcccaggcgt
    13261 taaatcgcag tttgtttgcc atcggtattg ataataccct gccggaagag ttcgccagac
    13321 tgtatccacg cctggtacgc accacccgcg cggcgctggc cggatttgaa agtgaatacg
    13381 gcgtccatct ttctgatgag gaaagtggtc tggtcgcggt gattttcggt gcctggctaa
    13441 tgcaggaaaa cgatctgcat gaaaaacaga ttattctact gaccgggaat gatagcgagc
    13501 gagaagcgca gattgagcag cagttacgcg aactaacgtt actgccgctc aacattaagc
    13561 atatgtcggt aaaggcattt ttgcagacag gcgctccgcg tggcgcggca ctgattattg
    13621 cgccttatac catgccgtta ccgctctttt caccaccgct gatctatacg gacctgacgc
    13681 tgacaacaca tcaacaggag cagatccgca aaatgctcga atcagcatga gggaacaact
    13741 ttcgggcgca aaaacattgc cggaagcgcc accagcgcca ttacccagaa cacgccgtgg
    13801 cccagatatt gatacaggaa accggcgaaa acggtcatga tagcgatact gccgcccatc
    13861 gcgacggcag agtaaaccgc ctgtaaacgg atgacttcgc taacctggcg agcagcaata
    13921 taacgcatgg cggccaggtg gcagaccgtg aaggtgccgc aatgcagaat ttgcaccact
    13981 atcaaccacg gcaacgccgt agttgctccc ataatgcccc agcgcactac gccgcaaatc
    14041 gccgagatca acagcatatc gcgagcacta caacggcgga aaagtttatt actcagcgca
    14101 aagataatga cttccgccac cacgcccagc gaccacaaat accccaccgc cgaggccgag
    14161 tagccagctg cctgccagta aatggcgcta aaaccgtaat aggccgcatg tgccccctgc
    14221 aataaacaaa cgcaggccag aaagcgccag ttctggcgaa ccagcgccaa ccacgcagac
    14281 caaccggtgc tctcctgctg gcggcttgcc ccttgtggct gaatcgtcgg acggatgaga
    14341 aagccgagca gcatggatgc cacgcccaac gtcaacagca cgaggatcac ccgataatca
    14401 aacatagtga ccagtttgcc cgtcagcgcc gagccaatga caaacgccac cgagccccac
    14461 agtcgcactt tgccgtaatc aagcgggaac tgcttttgcc acgtattcgc cagtgcatcg
    14521 gtcaacggta ccagcggtga gaaaaagagg ttaaagccaa tcatcaccag catcagccac
    14581 gctacgtgcg cccccgccca gaaggcgaca gcaaagagaa gtggcagcag tgccagcacg
    14641 cgcaaggcgg aaatcaggcg ggaaggatcg ctgacgcggg gcgcgatgag caaactcccg
    14701 agaaaacggg caaccagacc tgcccccaat aacaggccga tggtttctgg cgttaaacca
    14761 atccctttaa gccagacgct ccagaaaggt aaaaaaatgc cgtaactaaa aaagtatgtg
    14821 aaatagccga gcgccaacca gcgcgtggat tgcaaaacca tgagtccctc ccgtcaggag
    14881 gcgttagtct ggggttaatc gaatgttggt gcaagttgaa aaataagaat attcgagcct
    14941 gcttcccgat gtctgtaacg ccagttcaca agcactaaaa aactgcgatc cgcgacgcat
    15001 gttgcaaaaa taatgcgtaa ttatgccgtt acgcttgcca aacgttcctg cacaatggcg
    15061 atgaaatcgc gtaatgccag cttcatctcc cctttcttcc acgccatcag taaagcaatg
    15121 gaaggcacat ttccggcaat gggacgaaaa acaacctgcc cggtattaaa attattcata
    15181 taaccgggta tcaaagtgac gcccagcccc atgcccacca gattcatggt caccagaata
    15241 ttcgttgcca cctggacgat atttggctgg ctgttttctt gcgcaaacca cgctttaacg
    15301 atcggcgcaa gcgaaccgga atacgccgga tcggtactga cgaaattcac gccatccagt
    15361 tgcgcggcgg tgatctcttt ttcatgtgct aaagggtgat caaccggtaa caccaccact
    15421 aatggttcgt caaaaagctc cagataatca atctccgggc tataaacagg atggcgcatt
    15481 aagccgacgt cgagttcacc acggcgaatt ttttcctcct gttgcgtggt gattaaactc
    15541 accagctcaa tcaaggtgtc tggctgtctg agacgaaaca tcggtaatac ttttggcaat
    15601 aaattcactt ccgccgatgg cacaaagcca atggttaatt gtctgtcttc ctgaacaatt
    15661 ttccgcgccc gtaatttggc attttccgct tgttcgagga ttgccagcgc gtcctggaga
    15721 aaacattctc ccgccgccgt caacgcgact ttgcgcttat ccctcaccag taacggaaca
    15781 ccgacacagt tttcaagatc gcggatctgg ctgcttaacg aaggctgtga ggtatgcagt
    15841 ttttccgccg cacgggtaaa gttcagtgcc tgcgccactg cgacgaaata gcgtaaatgc
    15901 cgtagttcca tcaccttccc cttgttatcg aaaaaacgtc tcagctggta gaaattaact
    15961 atttcacata ttagcaacca accagcaaca tccttatggc acaaaaatag aaggtcaata
    16021 catcttatct ttcaggatta aaaaatatga ccacaccctc agatttgaac atttaccaac
    16081 tgattgacac ccaaaacggt cgggtcactc cgcgtattta taccgacccg gacatttacc
    16141 aactggagct tgagcgtatt ttcggtcgtt gctggctatt tctcgcccac gaaagccaga
    16201 tcccaaaacc cggtgatttc tttaacacct acatgggaga agatgcagtt gtcgtggtgc
    16261 gccagaaaga cggcagcatc aaggcgtttc tcaaccaatg ccgccaccgg gccatgcgtg
    16321 tgagttatgc agattgtggc aacagtcgcg cctttacctg cccgtatcac ggctggtctt
    16381 atggcattaa cggcgagttg atcgatgtac cgctggaacc tcgcgcctac ccacaagggt
    16441 tgtgtaaatc ccactggggg ctaaacgaag ttccttgtgt ggagagttat aaaggactga
    16501 tttttggcaa ctgggatacc agcgcaccgg gcctgcatga ttacctgggt gacattgcct
    16561 ggtatctgga tggcatgctg gatcgtcgcg aaggcggcac cgaaattgtc ggcggcgtac
    16621 aaaagtgggt gatcaactgt aactggaaat tcccggcaga gcagttcgcc agtgaccagt
    16681 atcatgctct gttcagccat gcttctgccg ttcaggtatt aggggcgaaa gatgatggca
    16741 gcgataagcg cctcggtgat ggacaaaccg cccgcccggt gtgggaaacc gccaaagatg
    16801 cgctgcaatt tggtcaggac ggtcacggta gcggtttctt ctttactgaa aaaccggatg
    16861 ctaatgtctg ggtcgatggc gcagtttcaa gctattaccg cgaaacctat gccgaagcag
    16921 aacaacgttt aggtgaagtt cgcgccctgc gcctggcggg tcataacaat atattcccca
    16981 cgctttcatg gctaaacggc actgccacac tccgcgtctg gcatccgcgc ggccctgatc
    17041 aagttgaagt gtgggcgttc tgtattactg acaaagccgc ctccgatgaa gttaaagccg
    17101 cttttgaaaa cagcgccact cgtgcttttg gtcctgctgg ttttctcgag caggatgact
    17161 cggagaactg gtgtgaaatc cagaaattgc ttaaaggcca ccgcgcccgc aacagcaaac
    17221 tgtgtctgga aatggggctt ggtcaggaaa agcgccgcga cgacggcatt cctggcatta
    17281 ctaactatat cttttcagaa actgccgctc gtggaatgta ccaacgccgg gccgatcttc
    17341 tgagtagcga aagctggcag gaagtgctcg ataaaaccgc cgcttaccag caggaggtga
    17401 tgaaatgagt gcgcaagttt cactggagtt acatcaccgc attagccagt ttctctttca
    17461 cgaagccagt ctgctggatg actggaaatt tcgtgactgg ttggcacagc tcgacgaaga
    17521 gattcgttac accatgcgca ccacggttaa cgcgcaaaca cgcgaccgtc gcaaaggcgt
    17581 ccagccgccg acgacctgga ttttcaatga caccaaagac cagctggagc ggcgaatcgc
    17641 ccgtctggaa acgggcatgg cctgggcaga agagccgccg tcacgcactc gtcacttaat
    17701 cagcaactgc caggtaagcg aaaccgacat cccaaacgta tttgccgtga gggtgaatta
    17761 tctactttat cgggcacaaa aagagcgcga tgaaacattc tatgttggaa cgcgtttcga
    17821 caaagttcgc cgtctggaag atgacaactg gcgcttgctg gaacgggata tcgtcctgga
    17881 tcaagcggtg atcacttccc ataacctgag tgtactgttc tgatgaatcg aatttatgcg
    17941 tgtcccgttg cggatgtgcc ggagggtgag gctctccgga tagatacctc gcccgtcatc
    18001 gccttgttca acgtgggcgg cgagttttat gccattaacg atcgttgcag ccatggtaat
    18061 gcgtcgatgt cagaagggta tctggaagat gacgccacgg tggagtgccc gctacacgcc
    18121 gccagttttt gcctgaaaac ggggaaagcg ttatgcctgc ccgccaccga tccgctcacc
    18181 acttatccag tacacgttga aggtggtgac attttcatcg acttaccgga ggcgcagcca
    18241 tgagcgatct gcataacgag tccattttta ttaccggcgg cggatcggga ttagggctgg
    18301 cgctggtcga gcgatttatc gaagaaggcg cgcaagttgc cacgctggaa ctgtcggcgg
    18361 caaaagtcgc cagtctgcgt cagcgatttg gcgaacatat tctggcggtg gaaggtaacg
    18421 tgacctgtta tgccgattat caacgcgcgg tcgatcagat cctgactcgt tccggcaagc
    18481 tggattgttt tatcggcaat gcaggcatct gggatcacaa tgcctcactg gttaatactc
    18541 ccgcagagac gctcgaaacc ggcttccacg agctgtttaa cgtcaacgta ctcggttacc
    18601 tgctgggcgc aaaagcctgc gctccggcgt taatcgccag tgaaggcagc atgattttca
    18661 cactgtcaaa tgccgcctgg tatcctggcg gcggtggccc gctgtacacc accagtaaac
    18721 atgccgcaac cggacttatt cgccaactgg cttatgaact ggcaccgaaa gtgcgggtga
    18781 atggcgtcgg cccgtgtggt atggccagcg acctgcgcgg cccacaggcg ctcgggcaaa
    18841 gtgaaacctc gataatgcag tctctgacgc cggagaaaat tgccgccatt ttaccgctgc
    18901 aatttttccc gcaaccggcg gattttacgg ggccgtatgt gatgttggca tcgcggcgca
    18961 ataatcgcgc attaagcggt gtgatgatca acgctgatgc gggtttagcg attcgcggca
    19021 ttcgccacgt agcggctggg ctggatcttt aaggaagcac gatgaaagaa aaaacgatca
    19081 ttattgtcgg tggcgggcaa gcggcggcaa tggctgcggc ctcgctacgc cagcaagggt
    19141 tcaccggtga gctgcatctg ttttccgatg agcgacatct tccttatgaa cgacctccac
    19201 tctcgaaatc catgttgctg gaagattccc cgcagttaca gcaggtgtta cccgctaact
    19261 ggtggcagga aaacaatgtt catctgcatt ccggtgtaac catcaaaacg ctgggtcgcg
    19321 acacacgaga gttagtgtta accaacggcg aaagctggca ctgggatcag ctttttatag
    19381 caaccggcgc ggcagctcga ccgctgccgt tgcttgatgc actgggagaa cgctgcttta
    19441 ccctacacca tgccggtgat gccgccagac tgcgagaagt tctgcagccc gaacggtcag
    19501 tcgtgattgt cggtgccgga actattggtc tggaactggc tgccagcgcc acgcagcgca
    19561 gcgcagcgca gcgcagatgt aaggtgacag tgattgaact ggcggcaacc gtcatgggcc
    19621 gtaatgcacc accgcccgtg caacgctatc ttttacagcg ccatcagcag gctggtgtgc
    19681 gcattctgct caataatgcc attgaacatg tggtcgatgg tgaaaaagta gaactgacgc
    19741 tgcaaagtgg ggaaacgctt caggctgatg tggtgattta cggtattggt atcagcgcca
    19801 acgagcaact ggctcgcgag gccaaccttg atactgccaa tggcattgtc attgatgagg
    19861 cttgccgcac ctgcgatccc gcgatctttg ccggtggcga tgtggcaatc actcgtcttg
    19921 ataatggtgc actacaccgc tgcgaaagct gggaaaacgc caataaccag gcgcaaattg
    19981 ccgctgccgc aatgttaggg ctaccgctac cgctactgcc gccgccgtgg ttctggagcg
    20041 atcagtacag tgataactta cagtttattg gcgatatgcg tggcgatgac tggctttgtc
    20101 gtggcaaccc ggaaactcag aaggcgattt ggtttaatct gcaaaacggc gtgcttatcg
    20161 gtgcggtcac gctgaatcag ggccgtgaga ttcgcccaat tcgcaaatgg atccagagcg
    20221 gcaaaacgtt tgatgcgaaa ctgctgatag atgagaacat cgcgcttaaa tcactgtaat
    20281 caggataatt agcgaatatc tcaatgcctg gggcgtggcg aggtgcaaga gtgtgtatta
    20341 cgtttaaatc acattatctt gcaaagggat tggttatgaa cacactacgt tattttgatt
    20401 ttggagctgc ccgccccgtt ttgttattaa ttgcccgtat cgccgtggtc ttaattttca
    20461 ttatttttgg ttttcccaaa atgatgggct ttgacggtac ggtccaatat atggcctcgt
    20521 tgggcgcgcc aatgccgatg ctggcagcga ttattgcggt agttatggaa gtgcccgccg
    20581 cgatattaat cgtgcttggc tttttcaccc gtccgctggc ggtgctgttt attttctaca
    20641 cgctgggtac ggcggtgatt ggtcaccatt actgggatat gaccggcgat gcggttgggc
    20701 caaatatgat taatttctgg aagaatgtca gtatcgctgg cgcgttcttg ctattggcaa
    20761 ttaccggacc gggggcaatt tctctcgatc ggcgttagga aagatgccgg atgcggcgtg
    20821 aacgccttat ccggcatttt ataaattaca gccactctac ccgcaacgac atctctgagg
    20881 ttgttgattc ccctggtgct agcgcaatga gatcaccccc ttccgggcga tgatgctcat
    20941 ccggcgcatg gctcatcggt tccagacaga agaaatcaaa cgcatatcct ttatcaaacg
    21001 cagggtcgga aacaaagatg aaataacacg gtgcaggtgg cgtcgtttcc atgatgatgg
    21061 cataaccttc ctgcggctgc tcgatgcggg cctgaccatt ccatccggca aaaccattgt
    21121 tcacccactg acgcggcaac ggcgcgagct ggttaaaatc cagttcctgc ggtagctgct
    21181 cgcaaaactc acccgccagc cactgctccc gttccagcca gtaaccgctc gcctgcgcct
    21241 gaatccgcgt ttgtggtgac aacggaaaat aaggatgcca gccggtacca aatggcagcg
    21301 tctctgcccc ttgattggtg acagagagcg tcaccgtcag cgtatccgcc gttaaatgaa
    21361 acgcctgact tacccgatag tgatagacac cgctgcgatg ttcatacacc agacacaaac
    21421 tatcatcgct gtgcgagaca cattgccact cgcccagcca gccatcgccg tgtagatagt
    21481 gcgcatccca ctcaacattc ggttgcagtt gatactcacg cccctgccag acaaaacggt
    21541 tgccgctcac ccggtttgca aatggcacca gcggaaaaca tgaggcatcg gttgccacac
    21601 cgcttttttt accaggacgt aagagcggcg tcgtatcgcg ccagaagcct tcgattacgc
    21661 cgccctggtc agaaacgtcc agctttagcg acccgtggga taaggtatag atggtcatcc
    21721 gctaactcct taatccggga agttaatcac aactttcccg catttgccgc tcgccatcag
    21781 cacataggca tcacctgcct gttctagcga gaagcgatgg gtaatggcgt tacgcggcca
    21841 cagcttccag tcagtcaaat cgtgggcgca tttttccata tggaacagac tggtcaccca
    21901 ggagccgata atccgccgtt gatggtgcat cagatcagcg ctgacctcga attccacttt
    21961 tccggtttca ccaatgtaaa ccacccgccc ccagtcagca gtggattgca gtgccagcaa
    22021 gcgacctgcg gcattaccgg aacaatcgag cgcaacatcc gcgccaccgt gggtaagttc
    22081 agcaattatc tgcggcagac cttcggtggt tgctaaatag ccgtgatcca tcccccctaa
    22141 ctgttttgcc atcgccagac gttccggcag catatcaacg ccgatgatcc gttttgcacc
    22201 gcgacctttc gccagcatca tcgccatcat gccgaccggc cccagaccga ccaccagcac
    22261 gttatcgctg ccggaaactt cgccgcgcaa aatcccttca taggccgtgc caacgccgca
    22321 actgataaac gcaccatctt cgtagctcag cgcatccggc aggaggatca gatctttttc
    22381 ttccgccagc agatattcgg catgaccgcc gtcacgctgc cagccgtaag ccgcttttcc
    22441 tttgccagta caagagatag gaaagccgcg gcggcagttc gggcaaaaac cacagccaga
    22501 aatgtgatac accagcacgc ggtcgccctc tttaaaatgg cggcagcctt gccccatcgc
    22561 cacaatctgc ccgcacggtt catgaccgtt gataaatccc tggtataacg gtttatcggg
    22621 tgccgccgcc gtggcacggt gttgatgata gatatagtgg acatcgcttc cgcaaatccc
    22681 ggaggatttc attttgatca gtacctggtt aatccccggc gtcggcaccg caacttcccg
    22741 cagatcgacg gttgaatttc ctagtaaata agcagccagc atcgttttca taaatccctc
    22801 attaacaata cgattaattt tcatccctgc agcacccgcg ccaggggcgt taaggtttag
    22861 cgtttcgctt tactgcttcg ctgggtcagc aagatattcg ccagcaccgc caccacgatg
    22921 atgacgccgc gtaccacctg ctggaaaaag gagttaatac cgagcagcac cagaccgtta
    22981 ccgattagcg taatcaccag cacaccaagc aatgtaccga acagggaacc gcgaccgccg
    23041 gaaagtgccg taccgccgac cacgaccgcg gcgatgacgt caaactccag accgtttgcg
    23101 gcacctgcgt taccggaacc gaggcgtgcc gccaacaaaa tgccggtcac cgccgctaat
    23161 aatcccgaaa gggtaaagat aagaatgcgc actcgacgaa cgttaatacc gcacaactgc
    23221 gccgccgtgg cattaccgcc aacggcaaaa accgagcgcc cgaaggcggt ttttcggcta
    23281 atgaacacaa acagcgcaaa caacaccatc atgatcagcg cggatactgg cacaccgaga
    23341 aattgtccgc ccagccagtc cagcacctcg ttttcgtcaa tcggcactgg cagtgcgttg
    23401 gtcataaaca gccccattcc gcgcagagcg ctccacagcc ccagtgtggc aacgaaactt
    23461 ggtacgttaa acacgccgcg cagcaccccg gcgagcgttc ccatcagcgc gcctaacagc
    23521 aacaccagca gacaagcaat cgccaacgga acttcaaatt gcagcaaaaa tgccaggcac
    23581 accgagacaa aagccaccat cggcccaacg ctgacatcaa tttcaccgga gataataatc
    23641 agcgtcatcg cccaggcggc aatcccaatg gtggcggcat cgcgcagcac gttcatctgg
    23701 ttattcaatg agataaagcc aggcgcgttc agggagaaga ccagataaag aatggcaatc
    23761 accaccagca aaccgatctc attaatatgg cgactgacaa attgtttgag cgagacgctc
    23821 ttgccctgcg gcaatggtaa tgacgaagca tacatcttca ggttcctcgg taaaattcat
    23881 cagtgcacag acagaatggc ggacatcagc tcatccacat tgaccggcga gtgaaactcc
    23941 tgcgagaacg tgccgtgctg taataacagg atgcggtcac acaccagcgg caattcctcc
    24001 acttcactgg agataaacac cacgcttttt ccttcggcag ccagctcacg gacaatacgg
    24061 taaatctgct gtttggcttc gatatcgacg ccgcgcgttg gctcgtcgag caacaaaatc
    24121 tggctggcgg catagaccca acgaccgatc accacttttt gctgattgcc accagaaagc
    24181 gtgccgatgg gtgtttcgct actggcagcc ttgaccgtca tccgctgcat cacctcttcg
    24241 gtcaggcggc ggatggtgga ccattgcagc acaccgttgg tgctgatttt ttgccgattg
    24301 gtcagcactg tattttcgtc aacgcctagc cagggaatga tccccgcttc tttgcggttt
    24361 tctggcgtat agccaatgcc gcgtttcagc atgtcgccgt aatcggggcg catgattttc
    24421 tcgccgttga taacaatttc gccctgttca tacgtctcca gcccaacaat cgccttcagc
    24481 aattcactgc gccctgcccc cagcaggcca gcaatgccaa gcacttcgcc acgacgtagc
    24541 gtaaagctga tatcctccag cttgggctta tggcgtaacg cacggacttc cagtacggcc
    24601 tgatccacaa tttcctgagg ggcaaccggc gcaatatcaa cgtgatcacg ccctagcatt
    24661 agcgacacaa tatgatgcgt ggaggtgttt tcgagcatca catcgcccgc cacctgacca
    24721 tcgcgcataa cggtggcata ggaggcaatg cggcggattt cttccatccg gtggctgaca
    24781 taaatcaccg ccacgcccag tgccgacatc tttttcaccg cactgatcac cagttcaact
    24841 tctgcactgg caagcgagct ggtgggttca tcaagaatga ccacgcgcgg ctcgcccttc
    24901 atcacccgtg caatttccac cagttgcttt tgcgccgggc ttagcgttga aacaagttgt
    24961 tcaggactaa cgtcaacgcc cagcgcctgt aagcaacgtt gggcatcctg cgccatttgc
    25021 aggtaatcaa tcatgccgtt gcggcgaggc cattgaccaa ggcagaggtt ttctgccacc
    25081 gtcagccctt ccaccagact taattcctga taaaccgcac gaacccccag ttcagcggca
    25141 cggcgagtca acgtagcttc gtcaccttcc agtcgcgtct cgccaatcca gatatcaccg
    25201 ctatccgggc gttcgctgcc ggtaagcatt cgaatgagag tcgatttgcc cgcgccgttt
    25261 ttacctaaca gcgcacgaac ttcgccttta ttgagcgtga agttaacgtt atccagcgca
    25321 acgacgccgg ggtaacgctt atttcctgcc accacttttg ctaccgggac tgcctctgtt
    25381 gccgtgaaca tagggacctc tgcgaatcag cgattagggc agaccatcaa cgtgcgttgc
    25441 cagccactgt ttaccatctt ccgttttggt atagagatcg attggcacct gaatcacttt
    25501 ttcaccgtcg gcttgtttat tgataacctt caatgtttgc gcgaaaacag cattgcccat
    25561 tttcttaccg gaaatatcca ctaccgcttt cagcacctga ttgttttcca gctcctgagc
    25621 aatttcggtt gtcatatccg aaccgaaaac agcaattttt ccggcctgat tttgattacg
    25681 taccgctttt accgcgccga gtgtcgcacc gcccgactcc cccataatgg cgttgagatc
    25741 cggcgtggag ataatcagtt tttcaccaac ggaaatcgct ttatctaaaa cagtcccttc
    25801 ctggttagcg acaatttgcg cgccgggaac gcgggatttt aatacttctt caaatccttt
    25861 acgtcgctgc acacaaactt caaaggcttc gcaattgatg acggcaattt tcggctggtc
    25921 aattttattg gcaataaaat aatcggcggc agcgttaccc agtttttacc aaattccagt
    25981 ggatcgccga ccagatacgc cgagacatat ttatcgacac ccttttgatt aatacaggtg
    26041 ttgtagcaaa tcaccggaat acccgcttca ctggcgcgac gaacggtacg gctactgcca
    26101 ttttcagaca ctgccgataa aataatggca tcgacattac gcgccacgag ggtatcaaca
    26161 aaggtacttt ctttcgaaat atccccctgg gcgttagttt caattaactg tacctgtact
    26221 gaagaatctt ttgccgcatc ctgaacgccc tgacgcactc cagcgtaata tccctgggta
    26281 tcaaggtata ttgcaccaat ggtcatttct ttatccgcag ccctggcaaa tagtgcgcta
    26341 cctaatagcg tagccattaa taatagatta cgggttgttc tcattttttt aggcatagtg
    26401 cctcctgtag ggtttttatt aacaacggct tattctaatt attttgtgat gagcggcagc
    26461 gcgtgccgct caaatattac agttaatagg gtttagtgaa cgtagttaaa aataaaaggc
    26521 atcacagtgg tgaataatgc cgctttcggc caggccggat taatcgccgt caattcagcg
    26581 cgagcagact catattgcgc aacctccccg agtcccgcgg acgccagcat cgccaccatc
    26641 aggcattttt ctttatgctg ctgttgtaaa tcgccataca gcgataacag gtcaggttgt
    26701 gaaacagcaa agaaatccgc ctcaatactg gttttcgcca tctcttgcgc ccactgtttc
    26761 atttcactaa acagttgctg tgcggtttgc tgttcaccca gcagtcgcag cgccattcct
    26821 tgccagaaga gataatcaac cggctgatcg ttgtaataac tgtggatgtt aatggtgcga
    26881 tcgccggtcg ccgccagacg taaacaacgc atcgcttcag tttcatcgcc ctgcgcgttg
    26941 gcgcatatcg cctgccagaa ccagatgtcg ttatcagttt gccccggtaa acggccttcg
    27001 cttaaattct ccggataata cagcgcggca tgaagcagtt cgctggcctg ctgcggctct
    27061 ctggcatcaa gatgctgcca ggcgcgtaat aactggttga ggataaactg actggtgacc
    27121 ttcccttccc cgccttccca cgggtggaat ttacgcgtgg cgagaatgtc cgccgctttg
    27181 tctgcctgac ccgtgagatg ccacaaattg agcagttctg cggtcatgtc gtcgcgtttc
    27241 agcgcaattt ccagattatt ttccagacgc gccagtcgtt tctccggtgt ggctccactt
    27301 aacttatcca gcaaatcccg ttcgaaaagc agacgtgcat cctgcggcgc aagctgataa
    27361 gcattatcaa gataacgcgc ggccagctca taatcgtgtt tcttattcca cgcatggatc
    27421 gctaacccgc gccagccgtc ggcaaactcc ggcgacatct ctacgcaacg ttgccataag
    27481 gcaatggctt tgttgtagct acgtttgttg tagtagaagc aggccagtaa atggcgagca
    27541 aaccaacact cttcaatact ctccagcgcc gccacttctt ccagcgtatt cgggaaacgg
    27601 acaaactgcg ggaagacatc aatggctttt gcgaccagtt cgccacgttc ggctttcggc
    27661 agcaagctgg cttgcaggta aagcggcagc gtgcgctggc agtccagtgc gttcagcatc
    27721 tctgccgcca gggtgggcat tccccagtta atcagttgcc cggcggtcat cagagcgtta
    27781 acgtcgcgtc cctgacacag accgcgccac tgcgcgaggg ctgattcgct acgaccatcg
    27841 aaccagttca gccaccacag agtggcgttc agcggataat cacgcagcag tttctcgcgc
    27901 tgcaaacgcg cgttgtcctg acgaccactt aacaccagca gcagattatg caagcaaagc
    27961 acttcctgat tggttgggca ggcacgaaga ctttgttggc aaaaatccag acccgcgtcg
    28021 aagttatcat tacgcgccgc cagtcgtgcc agaccataat agccgcctgc tttactattg
    28081 ccgctccaga ccgcacgcca gaaatcctct tcggcttgtt gatattgtcc ctgacgttcg
    28141 taagcactgg cgcgaatcaa acttgcttgt ccacactgcg gttttttgtt cagcgcatgt
    28201 gcacgtttca gcgcctgact ggcatacgcc accgcttgcg ggaaatctgc gcggttatat
    28261 tccagcatcg ccagcgccag gttacagcga taatccagcg ggtccagcgc cacgccgcgc
    28321 aggtagtaat cgaacggtga acggctggcg tgatgatatt gctccagatg ctgaccgata
    28381 aaccaggctt catctgtact ggtaatgtct tgtgctgcca gtggcgcttt ggcgacgtcc
    28441 ggcaacggca actcctgcgg ctgatgttcc tgataactca gtacaatatt gccgtcggca
    28501 tcggagagtt caatggtcag tctgtcagga ttgataccgt gcaacacgcc ctggatggcg
    28561 gtcgctggtg tcagggccac ggcatcatcg agcaacgcgt tgcatttgcc gatttcgcgg
    28621 atcgccagac ggtatccgtt caacggagag atggcataca gcccccactc aatcccccgc
    28681 tcactacgct ggagttttat caccgcatcg cgggaggcat tttgcaccat gcccagagaa
    28741 tgataaggca ggaaatactg ctcgaaacgc ttctcttcgt aagcatcaag ccaggtaaaa
    28801 tcaggctggt tatcggcaaa aataccggtc atcagttcga tatacgggcc gttattgtcg
    28861 gtcagactct tatcccacgc ctggccaaat tcactgtgtc cccaactcca ctgtttttta
    28921 cctggcgcaa tatggtggtt ggcaacgtgc agcaaaccgc catcttcatc gtgacaccac
    28981 gcgccgacaa aatcgtactg cgatttttcg gccatatatg aggttggaac aggcacattt
    29041 ttatagcgag aaatgtccac tccggcggag tagtccactt tgtagtaagt gccggtggcg
    29101 atggggaaag cggagacggc ccgtttgccg tgatcaaaca ccgcagtcac atccggtggg
    29161 aaaacgctct gatgcccttc accccctttc actgccgggt tgacccacca caagaaatga
    29221 cgcggcgtgg cgttgccgtt atagacgcgg ctggcgattt ccagtgccgc ccggtcaggg
    29281 cgcagggtga aacctgtcat cacctgtaaa ccatgcatcg gctccgtttc gccgacccac
    29341 accgtctgtg caccgtcttc atgggcttcg agggtgaaat caacgggcat aaaggtggtc
    29401 gggcgatggt gttgcggcca gttaaactca atcccgacgg agatccacgg tcccagcatc
    29461 cccaccagcg caggtttaat gacttcattg tgatagacaa aatcgcgctg tttcacttta
    29521 tcccatgcgc gatgcacccg accgcccagt tccggcagga tcatcacttt gatgtagtcg
    29581 ttttccagcc acaccgcctg ctaggatttc agggttttct gctcgctcag cgtatcggtc
    29641 acgccgtagg gataaaccgc gccggacgat ccctgataaa cgcgattttc caggaacatg
    29701 ggatgtatat cctgcggccc ggtttcatag gtcgggatct caacgcgctc ttgccacact
    29761 tttactggag tcatggtgcc ctcaatatta acaagacata ctgaattaaa agatttgtgg
    29821 cagtgtattg aacaatctgg caatgttttc gcggaataat cacgcaatta actaaacaag
    29881 gtttagtgaa gatgagagcc tgcattaaca atcaacagat tcgccaccat aacaaatgcg
    29941 tgattctgga actgctgtac cggcaaaagc gcgccaataa atcaacgctg gcccggctgg
    30001 cgcaaatttc gattccggca gtcagtaata ttttgcagga actggaaagc gaaaaacggg
    30061 tggtgaatat tgacgatgaa agccagacgc gcgggcatag tagcggtaca tggctgattg
    30121 cgccggaagg tgactggacg ctgtgcctga acgtgacgcc caccagtatt gagtgtcagg
    30181 ttgctaatgc ttgtttaagt ccgaaaggcg aatttgagta tttacagatt gatgtaccga
    30241 cgccgcaggc gctgctgtcc gaaatcgaaa aatgctggca tcgccaccgt aaattgtggc
    30301 cggaccgcac catcaatctg gcgctggcaa tccacgggca ggttgatccg gtgaccggcg
    30361 tatcgcaaac catgccgcaa gcgccgtgga caacgccagt tgaggtgaag tatctgctgg
    30421 aagagaagct gggcattcgg gtgctggtcg ataatgactg cgtgatgctg gcgctggcgg
    30481 agaaatggca aaataattcg caggaacggg atttctgcgt gatcaacgtt gattacggca
    30541 ttggctcgtc gttcgtgatt aacgagcaaa tttatcgcgg cagtttgtat ggtagcggac
    30601 agattggtca caccatcgtt aatccggatg gagtcgtctg cgactgtgga cgttacggca
    30661 gcctggaaac cgtcgcctcg ttaagtgcat taaaaaaaca ggcgcgggta tggctaaaat
    30721 cacaaccggt taatactcag cttgagcctg aaaaactgac aaccgcgcag ttaatcgctg
    30781 cctggcagag tggagaaccg tggatcacca gctgggttga tcgctctgcc aatgccattg
    30841 gtttgagtct gtataacttc ctcaacatcc tcaatattaa tcagatttgg ttgtacggtc
    30901 gcagttgtgc ctttggtgag aactggctta atactattat tcgccagaca ggatttaacc
    30961 cgttcgaccg cgacgaagga ccgagcgtga aagcgacgca aattggcttc gggcaattaa
    31021 gccgcgcaca acaggtgctg ggaattggct atttgtatgt tgaggcgcag ttacgacaga
    31081 tttgatggcg cgataacgta gaaaggcttc ccgaaggaag ccttgatgat cataaacgaa
    31141 aaattgcctg atgcgctacg cttatcaggc ctacacggag attgcaatat attgaatttg
    31201 caagattttg taggcctgag aaggtgttca tgccgcatcc aacatgaaca acgagcacat
    31261 tgtcagcaaa tcaccgtttc gcttatgcgt aaaccgggta acgtgcgcag atgtcgagaa
    31321 ctttaccttt gatgcgctcg ataacggctt catcattgat gctgtccagc acgtcacaca
    31381 tccagccagc cagttctttc gcttcggctt ctttaaagcc gcgacgggta atcgccggag
    31441 tacctacacg aataccggag gtcacaaacg ggctcttcgg atcgttcggt acgctgtttt
    31501 tgttgacggt gatgttagca cggcccagag cggcgtctgc ttctttaccg gtcaggtttt
    31561 tatcaaccag atcaaccagg aacaggtggt tatcagtgcc gccggaaacc actttgtagc
    31621 cgcgctcgag gaacacttct accatcgctt tagcgttttt cgcgacctgc tgctggtaag
    31681 ttttgaactc aggctccatc gcttctttca gagcaaccgc tttaccggcg attacgtgca
    31741 tcaacggacc gccctgacca ccagggaaaa cggcagagtt cagttttttg tacagctctt
    31801 cgctaccacc tttcgccaag atcaggccgc cgcgcggacc cgccagggtt ttgtgagtgg
    31861 tggtagtaac aacgtgagca tgaggaaccg ggttcgggta gacgccagca gcaaccaggc
    31921 ccgcaacgtg cgccatatca acgaacagat aagcaccgat gctgtcagcg atttcacgca
    31981 ttttcgccca gtcaaccacg ccggaatatg cagagaaacc accgataatc attttcggct
    32041 tgtgttcttt ggcttgtttt tccagatcgg cgtaatcgat atgaccggta gcatcgatac
    32101 cgtaaggaac gatgttgtac agtttaccgg agaagttaac cggagaaccg tgagtcaggt
    32161 gaccgccatg cgccaggttc atacccagaa cggtatcacc tggttccagc agcgcggtgt
    32221 agaccgcaaa gttagcctgg gagccggagt gcggctggac gttagcgtag tcagcgccga
    32281 acagttcttt cgcacgatcg atcgccagtt gttcaacgat atcaacatac tcgcaaccgc
    32341 cgtagtagcg tttgcccgga taaccttcag catatttgtt ggtcagctga gaaccctgcg
    32401 cctgcattac gcgcgggctg gtgtagtttt cggaggcgat cagttcgatg tgctcttcct
    32461 gacgtacttt ttcctgctcc atagcctgcc acagttcggc atcataatcg gcaatgttca
    32521 tttcacgctt taacatccgc atctcctgac tcagctaaca ataaaatttt tggcctttat
    32581 aggcggtcct gttggacaac ggcgaacagt ataaccgaat cattgtgcga taacaggtct
    32641 tgacaaagga atttacgcaa acgattacct tcaggctacg caaggctttg gagaataaag
    32701 agcttgcaac cggaaacgga tttcttttca ggtttgtgat gcaaattttt cacttcatca
    32761 cattctttct gaaaaacacc aaagaaccat ttacattgca gggctatttt ttataagatg
    32821 catttgagat acatcaatta agatgcaaaa aaggaagacc atatgcttga cgctcaaacc
    32881 atcgctacag taaaagccac catcccttta ctggtggaaa cggggccaaa gttaaccgcc
    32941 catttctacg accgtatgtt tactcataac ccagaactca aagaaatttt taacatgagt
    33001 aaccagcgta atggcgatca acgtgaagcc ctgtttaacg ctattgccgc ctacgccagt
    33061 aatattgaaa acctgcctgc gctgctgcca gcggtagaaa aaatcgcgca gaagcacacc
    33121 agcttccaga tcaaaccgga acagtacaac atcgtcggtg aacacctgtt ggcaacgctg
    33181 gacgaaatgt tcagcccggg gcaggaagtg ctggacgcgt ggggtaaagc ctatggtgta
    33241 ctggctaatg tatttatcaa tcgcgaggcg gaaatctata acgaaaacgc cagcaaagcc
    33301 ggtggttggg aaggtactcg cgatttccgc attgtggcta aaacaccgcg cagcgcgctt
    33361 atcaccagct tcgaactgga gccggtcgac ggtggcgcag tggcagaata ccgtccgggg
    33421 caatatctcg gcgtctggct gaagccggaa ggtttcccgc atcaggaaat tcgtcagtac
    33481 tctttgactc gcaaaccgga tggcaaaggc tatcgtatcg cggtgaaacg cgaagagggt
    33541 gggcaggtat ccaactggtt gcacaatcac gccaatgttg gcgatgtcgt gaaactggtc
    33601 gctccggcag gtgatttctt tatggctgtc gcagatgaca caccagtgac gttaatctct
    33661 accggtgttg gtcaaacgcc aatgctggca atgctcgaca cgctggcaaa agcaggccac
    33721 acagcacaag tgaactggtt ccatgcggca gaaaatggcg aagttcacgc ctttgctgat
    33781 gaagttaagg aactggggca gtcactgccg cgctttaccg cgcacacctg gtatcgtcag
    33841 ccgagcgaag ccgatcgcgc taaaggtcag tttgatagcg aaggtctgat ggatttgagc
    33901 aaactggaag gtgcgttcag cgatccgaca atgcagttct atctctgcgg cccggttggc
    33961 ttcatgcagt ttaccgcgaa acagttagtg gatctgggcg tgaagcagga aaacattcat
    34021 tacgaatgct ttggcccgca taaggtgctg taatttgatg ttgccggatg gaaacatccg
    34081 gcaacccttg acgcggttta aattgccgcg tcgtcctctt caccggtacg gatgcgaatg
    34141 acccgagcca cgtcaaagac gaagatttta ccgtcaccga ttttgccggt ttgcgccgtg
    34201 cgaataatgg tatcgacaca ggtatcgaca atgtcgtccg gtacgacaat ctcaattttc
    34261 actttcggca gaaaatccac catatactcc gcgccgcggt acagctcggt atggcctttc
    34321 tggcgaccaa agcctttcac ttcggtcacc gtcatgccgg taataccgac ttcggccagt
    34381 gcttcgcgga catcgtccag cttgaagggt tttataatcg catcaatctt tttcatgcta
    34441 ttccttgaaa aggttgcctg tcttttgatc tgctaaacgt aacacataac gccaattcat
    34501 tccttgaaat cgtttgcatc cagctcgtgt cgggaaagca gtttataaaa ttctgtccgg
    34561 ttgcgccccg ccattctcgc cgcgtgggtg acgttgcctt tggtgatttg cagcagctta
    34621 cgcaagtagt tgagttcaaa atgattacgt gcctcaacaa aggttggcag cgccgtattt
    34681 tcaccctcca gcgcctgctc caccagcgca tcactaatca ccggagatga ggtcagcgcc
    34741 acgcactgtt caatcacgtt gaccaactgg cgcacattac ccggccagct cgcggtcatc
    34801 aggcgtttca tcgcatcggt agagaacgcg cggacaaacg gtttatgtcg ctctgccgcc
    34861 tggcgcaaca ggtgatttgc cagtagcgga atgtcttctg tgcgctccgc cagtgccgga
    34921 attttcaggc tgacaacgtt gaggcggtaa tagagatctt cgcggaattc cccgcgcgcc
    34981 atcgcttttg acagatcacg gtgagtggca gaaataatcc gcacattgat atcaatatcg
    35041 cggttactgc ccagcgggcg cactttacgc tcctgcaaca cgcgcagcag tttgacctgt
    35101 aacggcgcag gcatatcgcc aatttcatcg agaaatagcg ttccgccttc cgccgcctgg
    35161 aataaacctt cgcgattgct gacagcgcca gtaaacgcgc cacgcgcatg accaaacagc
    35221 tccgactcca gcaattgctc gggcaatgcg ccacagttaa tagcaataaa tggtttgctg
    35281 ttgcgcgggc tggcgttgtg gatagcctgg gcgaaaatct ctttcccggt gccgctctga
    35341 ccgttaatca aaacgctgac gtctgattgc gccaccagcc gcgcctgttc cagcaaacgc
    35401 agcatcagcg ggctgcgggt gacaattgcc tcgcgccagc gttcatcggt ggctggcgcg
    35461 gattgctcca gcgcatcgtc aattgcctga tatagcgcat ctttatcgac aggcttggtg
    35521 aggaaactaa aaacgccctg ctgtgttgca gcaacggcat cggggataga accatgcgca
    35581 gtaagaataa ttactggcat tcccggctgc actttctgga tttcagcaaa tagctgcata
    35641 ccgtccattt catccatccg caggtcgctg ataactaaat ctactttttc gcgattcagt
    35701 acccgtaatc cttcagcgcc actttccgcc gtgaccacac tgtagccttc gctggtcagg
    35761 cgcaggccaa gcagtttcag caatcccgga tcgtcatcga ccaataataa atgcgcaggt
    35821 ttatggctca tcaggagtga cctcatgggt ggatggcgcg ggcttttcac tctcgtgcgg
    35881 cgtatccggc gagaaatttc cagccggttt gcgggtcgag agctggcgtt caatatcggt
    35941 caggttttcc agcttgcggg tggtgagttc cagttgctgc tgtagaacgt gatgttgctg
    36001 gcgcaatgta tccagctcgc tgtcgctgga ctgctggagt ttactgtaac gttggcgctc
    36061 ttccgccagt tgcagttgca gcgcctgacc gtcgcgccag agttgataca gtgggcgaac
    36121 ctgtgccggg atctcggtac ttaacgcttc aatacgcgcg accagctggc ggcgctcata
    36181 cggcgtaatt ttggcgtcgg cgagcaaaat cccttgttta aaggtatttt gccagctgcc
    36241 gtcgtcatat tggcgagctt gctgacgcga ctgcgcaggc attaaacgat cagcacaatc
    36301 catcgcccgc agccagtaaa gcggattggt ttcggttgat ttaccttgca gcgcccagat
    36361 gtcgctacat tcagtagaaa gatagtcagc cagttgataa accggaattt tttcttctgc
    36421 tggcgtatca atggctggct tattgtgatt ctgcacgcaa cccagcaatg ccagacatgg
    36481 cagccctgcc agccacagcc gtcggggcaa taatcgttga aaaatgtgtc gcatattcac
    36541 cagacttaaa gcctatccca gtgggcgtaa ttgttgcaga cagtctggac atggacagcg
    36601 cggagaaacc ggggcgtaca tatcgtacgt gaagatttcg agcactaccc ggggccgaac
    36661 tgacaaataa aatagcctga taggataggc tgttagcatt atttcgtgtt tttcgacgac
    36721 ggtaattcaa tgcggaaaca aacgtcttgc ccgctctcgt cgaccagata cagttcccct
    36781 tgcatacggc gaatacaatc cctggcaatg cttaatccca gaccgctgcc cttcaccgcc
    36841 ccttttcgct ggtggcttcc ctgaaaaaag ggttcgaaga tcatggcgcg ttcctcttgc
    36901 ggaatgggcg tgcctgtatt gatgacatca atataaaccc gcgcaccatg taaactgctg
    36961 cgaaggcaaa tgttaccgga ttcagccccg tagtgcaccg cattggagta aagattatcc
    37021 agtacgctca tcagcagcat tggctccgcc aggcaagctg ttgctttgag atcgacgtcg
    37081 gtatgcatca ttttagcccg tgcgggcagg ctatgagcag aaaccactgt ctccaccagc
    37141 ggtgctaatt caacattctc cagttccacc gcactgtccg cctgtttacg gttgtaatca
    37201 agcagttgtt cgatcagttt ttgcaaattg cagctgctgc tatcaagaat gctcaccacc
    37261 tctttttgct ctggcgtaag cggcccgaca acctgatcag ccagtaattc agtgccctcg
    37321 cgcatactcg ccagtggcgt ttttaattca tgagataaat gtcttaaaaa ttgatggcgt
    37381 tgggattcca gccatgacag gcgctcactt aaccaaagaa tacgttgccc aaccgagcgt
    37441 aactcgctcg gtccacagaa cgaaacgcta ttgcccagag aacgcccttc ccccagacgg
    37501 ttgatcatgc gctcgatatt tttcaccggc ccgataatca tccgcgtgaa aagtagcacc
    37561 atcaccagac tcaccagaaa tagcaccagc gattgccaac caaaatattg cccacgttcg
    37621 gcgatttcac gctgaagttg ctgcccacga gagaacacca ctgtgcgcgt ggcctgcacc
    37681 atttcggtat tggcactggc aaaggcttca agacgcgcgg cggcggcagc atcgggacca
    37741 ctgttgttac actgaagttg ggccagattg ttcaaatcct gacgcaatgc ctggtaaagt
    37801 ttatcgtctg gcagtacgcc tgcgtgggca tcgagcattt cgctgtaacg cttgcgctgg
    37861 ctttgataaa ccttcgcaag cgtagggtcg tccagcacgc aatactgacg gtagctacgc
    37921 tccatctcca gcgccgcgtt ggtcatcgct tcactacgcc gggcatcaat aagcgtagtg
    37981 cggttaacca gcgccgcctg atcgctaagc gcattcaggc tttgccaggc ttgccatgcc
    38041 agcaccaaca ggggtagtag aattagcaaa aatgccagca ttaccagttg tcgtaatgag
    38101 cggggaaaaa cgggccagcg tttcaaggtg ttactctcgt cagacgcgaa tagcctgatg
    38161 ctaaccgagg ggaagttcag atacaacaaa gccgggaatt acccggcttt gttatggaat
    38221 aaggcggtgc ctaactcgac gtttcgcccg atggttgata tagctacgct gatatcagaa
    38281 gttggacggc aggcaccttg ttgtgcgtca ttcgaatttt atgtagcacg tcccgaaggg
    38341 gctgacataa gtcggtgaat gagccactgc ttactattat gcagtaactg tgccaataaa
    38401 gaaaatagtt tggtaacgta ctgattatac gttgctttga gggatttatg gcttcttcgc
    38461 tatttgaatg taaatcagcc actgtgtcgc taaaaagaga caacttaaga taaacttatt
    38521 agattacaat aaaatcaatc atttaagtgt cgccagaaag cgacacggta aaccacccat
    38581 tgtcgtgatt tacagacaca aaaaagctcc cggagttggg agcttatgat agtggttggt
    38641 gcttaatgcc gcatccgggc tgcaaaacca atgaactgat gacttacccc aactgcttac
    38701 gcgcattgcg gaaaatgcgc atccatgggc catcctcgcc ccagttttcc ggatgccagg
    38761 agttgctgac agtacggaaa acacgttccg ggtgcggcat cataatggtg actcgaccac
    38821 tttcagtcgt aaccgccgta ataccgttcg gtgaaccgtt cgggttagcc gggtaggttt
    38881 cagtgacttt gccgaagtta tcgacatagc gcagtgccac cagccctttg ctttccagtg
    38941 ccgccagatg cgccgcatca cgcacttcca cgcgcccttc accatgagag acagcaatcg
    39001 gcatttgcga gcccaccatc ccctgcaaca gcagagacgg gctttgggtt acttcaacca
    39061 ggctgaaacg cgcttcaaag cgatcggagg tgttacgcac aaaacgtggc cacaactcac
    39121 tccccgggat cagttcacgc aggttagaca tcatctggca accgttacat acccccaggg
    39181 ccagcgtttg cggacgatgg aagaaggttg caaactcatc gcgtacacgg tcattgaaca
    39241 ggatagactt cgcccaacct tcaccggcac ccagcacatc accgtaggag aaacctccgc
    39301 acgcgaccag ggcgtgaaaa tcctccagtc ccgtacgtcc agtcagcagg tcgctcatat
    39361 gcacgtcgat agcatcaaag cccccacgtt ggaaagctgc cgccatttca acatgcgagt
    39421 tcacgccctg ctcacgcagt acggcaactt tcggacgtgc gccagtggca atatacggtg
    39481 ctgccacatc ttcgttgata tcgaacgaca gttttacatt caggcccgga tcggcgtcgt
    39541 tagatttcgc ctgatgctcc tgatcggcac actccgggtt gtcacgcagg cgctgcatct
    39601 gccaggtagt ttctgcccac cagacacgca acgtagtgcg gctttcgctg aatacagtct
    39661 gcccgttggc ggtaatcaca aaacggtcac cggaaaccgc ctgcccaaca taatggacgc
    39721 aatccgcaag cccatgctgt gccagtacgg actcgaccgc ttcacggtca gcggcacgaa
    39781 cctgaatcac cgcacccagt tcttcgttaa acaacgccgc caggcgatcg tcacccagag
    39841 aggcgatatc cgcgtcaatg ccacaatgac ctgcaaaggc catttccgcc agcgttacca
    39901 gcaggcctcc atcagagcgg tcgtgatacg ccagcagctt acgctgtgca accagcgcct
    39961 gaatcgcgtc atagaagcct ttcagttgga caacatcgcg tacatctgcc ggtttgtcgc
    40021 caagctgacg ataaacctgc gccagcgccg ttgcgcccag cgcgttattg cctttgccca
    40081 aatcaatcag cagcagtgcg ttatcttcgg tacaaagctg cggcgtgatg gtgtgacgta
    40141 catcttccac gcgggcaaat gcagaaatca ccagcgacag cggcgacgtc atttcgcgct
    40201 cttcgttacc ttcctgccag cgggttttca tcgacataga gtctttaccc accgggatcg
    40261 tcaggcccag cgccggacaa agttcttcac ccacggcttt aacggcttca tacaggcccg
    40321 catcttcacc agggtggcct gccgccgcca tccagttggc ggaaagttta atgcgtttga
    40381 tatcgccaat ctgtgttgcg gcaatattgg ttaacgcttc accgaccgcc agacgggcag
    40441 aggcggcgaa atccagcagc gcaaccggcg cacgctcgcc aatcgccatc gcttcaccgt
    40501 agtagctgtc gaggctggcg gtagtgaccg cgcagttagc gaccggcacc tgccacggcc
    40561 ccaccatctg atcgcgcgcc accatgccgg ttacgctgcg gtcaccgatg gtcaccagga
    40621 aagttttctc cgccacggtc ggcagatgca gtacgcgttt caccgcgtct gcaatggtga
    40681 tcccttcacg gaccagcgca tcgcccttcg ctttcagcgt ttgtacatcg cgggtcatct
    40741 tcggcgtttt accaagcagg acgtccagcg gcagatcgat cggctgatta tcaaaatgac
    40801 gatcgtgcag agaaagatgc agttcttcgg tcgcttcacc aatcaccgcg tagggtgcgc
    40861 gctcacgctt acacagttcg tcaaacagcg gtaattgatc ggcagcaacc gccagcacgt
    40921 agcgttcctg ggattcgtta caccagattt ccagcgggct catgcccggt tcgtcgctta
    40981 gaatatcgcg cagttcaaat ttaccgccgc gtccgccgtc gctcaccagc tccggcatcg
    41041 cgttagaaag accgcccgca ccgacgtcgt ggataaacag aattgggtta gcatcaccaa
    41101 gctgccagca acggtcgatc acttcctggc aacgacgctc catctccggg ttgtcgcgct
    41161 gtacggaagc aaagtcgagg tcggcatcag actgaccaga cgccatggaa gacgccgcgc
    41221 cgccgccaag accgatgttc attgccgggc cgccgagaac gaccagcttc gcaccgacgt
    41281 tgatctcgcc tttttgtacg tgatcggcgc gaatgttgcc gatcccgccc gccagcatga
    41341 tcggtttgtg ataaccgcgc agctcttcgc cattgtggct gttcactttt tcttcataag
    41401 tacggaagta gccgttcagt gccggacgac caaattcgtt gttaaacgcc gcaccgccca
    41461 gcgggccttc ggtcatgatg tccagcgcgg taacaatgcg ctcaggctta ccgaaatctt
    41521 cttcccacgg ctgttcgaag ccaggaattc gcaggttgga tacggagaaa ccaaccaaac
    41581 cagctttcgg cttcgcgccg cgcccggtgg caccttcatc gcggatttca ccgccggaac
    41641 cggtcgccgc gcccggccac ggagaaatcg ccgtcgggtg gttgtgagtt tcgactttca
    41701 tcaggatgtg cgccggttcc tgatggaaat tgtagcggcc cgtttcgtgg tcagcaaagt
    41761 agcggcccac ttcagaacct tccattacgg cggcgttatc tttataagca gagagaacgt
    41821 gatctggcgt ggtttcgaaa gtatttttga tcatcctgaa cagcgatttc ggctgttgtt
    41881 caccgtcgat aatccagtcg gcgttaaaaa ttttgtggcg gcagtgctca gagttcgcct
    41941 gagcgaacat atacagctcg atgtcgttcg ggttacgacc aagctttgtg aaagcatcct
    42001 gcagatagtc aatttcatct tccgccagag ccaagccaag acgcaggtta gcgtcgatca
    42061 gcgcctgacg gccctgcccc agcaaatcaa cgcttgtgac cggagtcggt tgatggtggg
    42121 caaacaactg ctctgcatca tctaaatcaa aaaagaccgt ttccatcatg cggtcgtgca
    42181 gttcagcggt aacctgctgc cattgttcat tggtcagcgt accggcttct atatagtaag
    42241 caacgccgcg ctcaaggcgg tttacctgtt gtagcccgca gttatgggca atatcggtcg
    42301 ctttcgaaga ccagggagag atggtgccag gacgcggggt caccagcagg agtttgcctt
    42361 gcggggcgtg gctggcgagt gccggaccat atttcagcag gcgttcaagt tgtgcgtgct
    42421 catcatcgtt taacggcgca ttgaggtcag caaaatggac atactcggcg taaatattgt
    42481 gaaccgggag cctggcagcc tgaaaacgtg ccagcagttt gttgattcgg aatgccgaca
    42541 gtgcaggcga accacgcaga atttccatca taagtctctc gtcttctaag ctttcggtgt
    42601 acccaagggg ggggaaacgg gcgtcattat aaagaatctg atgcgctgac gaaaccgttt
    42661 gcgtggaaat aaaatcacca tcgtgaatta gcaacgcgtg ccgccaatgg ctgtaataag
    42721 ttgccatctg gcgcaggttt acgcaaaatg ccgctcattt atgagtaaac ctttcactat
    42781 tattacgttt tttcaagctg ggacgcgcac gacacagaga attaactaat tgaaaaaatt
    42841 aaagattaat tatctgttca tcggcattct ggcactgctg ctcgcggtcg ctctctggcc
    42901 atccattccc tggtttggta aagccgacaa ccgtatcgcc gccattcaag cgcggggaga
    42961 gttgcgtgtg agcaccattc atactcccct gacttataac gaaatcaacg ggaaaccttt
    43021 tggcctggat tacgaactgg cgaaacagtt tgccgattac ctcggcgtaa aactgaaagt
    43081 gaccgtgcgg cagaatatca gccagctgtt tgacgacctt gataatggta acgccgacct
    43141 gctggcggca ggactggtct ataacagtga gcgggtaaaa aattatcagc ctggccctac
    43201 ctattattcc gtgtcacaac aactggttta taaagtgggt cagtatcgcc cacgtacgct
    43261 gggcaacctg acggcggagc aactcaccgt tgcaccgggt catgtggtag ttaacgatct
    43321 ccagaccctg aaagaaacaa aattcccgga attaagctgg aaggtagacg acaaaaaagg
    43381 ctctgcggaa ttaatggaag atgtcatcga aggaaaactc gattacacca ttgctgattc
    43441 tgtcgccatc agcctgtttc agcgcgttca cccggagctc gccgtagcgc tcgatatcac
    43501 cgatgaacaa ccggtgactt ggtttagccc gttagatggc gataataccc tttccgccgc
    43561 cctgctcgac ttcttcaacg aaatgaatga agacggtacg ctggcacgca ttgaagagaa
    43621 atacctgggg catggcgatg attttgatta cgtcgatacg cgcacatttt tacgcgccgt
    43681 cgatgcggta ctgccgcagt taaagcccct gtttgagaaa tacgccgaag aaattgactg
    43741 gcgtttgctg gccgctattg cttatcagga atcgcactgg gatgcacagg ccacttcacc
    43801 gacgggtgtg cgcggcatga tgatgttaac caaaaatacc gcgcaaagcc tcggcattac
    43861 ggatcgtacc gatgccgaac agagcatcag cggtggcgtg cgttatttgc aggatatgat
    43921 gagtaaagtg ccggaaagtg tgccggagaa cgaacggatc tggtttgccc tcgctgcgta
    43981 caatatgggc tatgcgcata tgctggatgc ccgcgccctg acggcaaaaa ccaaagggaa
    44041 tcctgacagt tgggctgacg taaaacagcg tctgccttta cttagccaga aaccctatta
    44101 cagcaagctg acttacggct acgctcgtgg gcatgaagcc tacgcttatg tcgaaaatat
    44161 tcgtaagtat cagattagcc tggtgggtta tctgcaagag aaagagaagc aggctacaga
    44221 agcggcgatg caactggcgc aggattatcc ggcggtatcg cctacggagt tgggcaaaga
    44281 gaaatttcct tttctctcgt ttctttccca gtcgtcatca aactatttga cccattctcc
    44341 ctctctgctg ttttccagga aagggagtga agagaaacaa aattaatccg tcgaggattg
    44401 cgcttttttc tgcgctttaa tttcctggcg gcgcatgcga aagaagtcac tgagcaacgc
    44461 cgcgcactca tccgccagta ttccttccgt aatttccact cggtgattca tacccggatg
    44521 atgcagcaca tccattaaag atcccgcagc gccagttttc gcgtcacgcg caccaaagac
    44581 cacgcgacca atgcgactgt ggatcatcgc tccggcacac attacacatg gttcaagcgt
    44641 gacatacaac gtggcgtcga tcagacgata attttgcatc accagaccac cctgccgcag
    44701 ggccatgatt tctgcatgtg cggtgggatc atggcgacca atcgggcggt tccagccttc
    44761 gccgattacc cgattgttat gcactaatac cgcgccgacc ggcacttccc gctcatccca
    44821 ggcacgtttc gccagcgtca gcgcgtgacg catccaatat tcgtggctaa attcgacttc
    44881 agacaaaaag aaaactccgg ttataaaagc gcggcgcatt atacacggac gctatgcttt
    44941 actctattcc agttgctgga gttcaccgcg cggggttact cgccaacgat gctgacagaa
    45001 ataaagcagc ggattgtcct gattactgtc gctatagcca ctgtacagcc gcagcggcgt
    45061 gccgattttg cgctccagtt gtgcgacctt ttcatgtccc agacaacgca tcgtcaatac
    45121 ccaaccacca tagccacgct gaatttggct ggcgataaga ttaacccgcg gcagccaggg
    45181 cgtatcgaaa taaaccgctt caaccagcgg ctgcggagag ccggtaatca accagatatc
    45241 agcatcggaa cttaacaggt aggtggttaa tcgctcctga accagcggaa aggcggtaac
    45301 attgtcgcga aaccagcgca cgaaatcggc ctgcaacgtc tgtaaacgtg cttcgctgtg
    45361 accaaaagtg cacccccaca gaagcagact catcggccag cgtgccgcac gaccttttat
    45421 caataacgca atggctataa tcggtaacaa cggcaggaca agtaacgcat tcagcggttg
    45481 gcgacgcagt aaatagcgca gaaaactgcc gaacatatcc tgctgatgca atgttccatc
    45541 taagtcaaaa aacaccacac gacgctcgtg agttgccaaa ccatactcct ctggactgaa
    45601 acatcctgat taattttcgc tactacatag cctaacagat agatcatcac ttttccggca
    45661 acaatcgacc gcatcatggc taactggaat ttttaattca tggcaattag cggcaatgga
    45721 atataaaatt cactcgcgtg tgtctcatat tgcctgttgt cgccactatt tcatcgcgag
    45781 gaaagatatg aacggcttac ttcgtatccg tcagcgttac caagggcttg cccagagtga
    45841 taaaaaactg gcggattatc tgctgctaca acctgatacg gcgcgccatt taagctctca
    45901 gcaactggcc aacgaagccg gagtcagtca gtccagcgtc gtgaagttcg cgcaaaaact
    45961 cggctataaa ggttttccgg cgcttaagtt ggcgttgagt gaagcgctgg caagccagcc
    46021 ggaatcgccc tccgtgccca ttcataacca aatccgcggt gatgatccgt tacggctggt
    46081 cggcgaaaaa ctgattaaag aaaataccgc cgctatgtac gcaacgctaa acgttaatag
    46141 tgaagagaaa ctgcatgaat gcgtaacaat gttgcgctct gcgcggcgga taattctgac
    46201 cggtattggc gcttcgggtc tggtggcgca aaactttgcc tggaagctga tgaagattgg
    46261 cttcaatgct gccgcactgc gcgatatgca tgcgctactc gcaacagtac aggcgtcgtc
    46321 ccctgacgat ctgttattag ccatttccta caccggtgta cgacgcgagt taaacctggc
    46381 ggcagatgag atgctgcgag tgggcggaaa agtgctggcg attaccggct ttactccgaa
    46441 tgccctgcaa cagcgtgctt ctcattgcct gtataccatt gccgaagaac aagcgacaaa
    46501 cagtgcttca atctctgctt gtcacgctca gggaatgtta acggatttgc tgttcattgc
    46561 gctgattcag caggatctgg aactggcacc agaacgtatt cgtcatagtg aagcgctggt
    46621 gaaaaaactg gtctgagtaa agaatgcgcg tataatgccc gcccggtttg tgttgttttg
    46681 agagtttcct tatggcgctg ttaatcacta aaaaatgcat caattgtgat atgtgtgaaa
    46741 tcaaataaaa acacatttat tttcatagtg atacagcaat gtgttattga atgtgttgtt
    46801 ggatttatgg tgcaaaaaaa ttttgcataa gttcagcaac acaaagaaca ccactttctt
    46861 taacgtcctc cgcgccctaa aggacggggc tttacggcgc aaaaggcctg tgacatacaa
    46921 cattaacttt tcagtcaacc gtagcacgtt cttgtatacg ccgtgtctgc ggttttatat
    46981 aaccctggct ggaaacctct tatacaaagt cgattcgcca acatcataga tgatcgccac
    47041 cttctggcga gaaactcctg atgcaattag tcgcccggcc tgcgcccatt attctggtga
    47101 aagtttggga cggcgaccgc caattcgtcc ctgtgcgcga gcagcttcca gtcctgcttt
    47161 tgttcgttca acaatcagtt cacgttccat ttcagccagg gcacccatca catggaagaa
    47221 aaagcgtccc attggtgtgc tggtatcaat tgaatcagtc agactacgaa agttgatgcc
    47281 tcgttcgcgc aacgcctcca ccagcacgac aagatgccgc atactgcgcc ccagccgatc
    47341 cagcttccag acaaccagtg tgtcgcctgc cgataatgtc ttgagcagtt ttttcagccc
    47401 cggtctttcg gactttgtac cacttatctt gtcttcaaaa atcagctcgc atcctgcaca
    47461 gttcaacgca ttacgttgta gatcggtatt ctggtcattt gttgatac
//
LOCUS       DAJTSA010000024        47425 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000024 DAJTSA010000000
VERSION     DAJTSA010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47425
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.024"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..654
                     /locus_tag="OO369_001411"
     CDS             <1..654
                     /locus_tag="OO369_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5981740.1"
                     /translation="KKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEHLLPHF
                     LEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHKISHDV
                     FEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATILGDGV
                     VHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSASGKK"
     gene            1152..1334
                     /locus_tag="OO369_001412"
     CDS             1152..1334
                     /locus_tag="OO369_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR5981741.1"
                     /translation="MTEHRGGAGNFAEDRQKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQNSHGGGRKSDS"
     gene            1413..1913
                     /locus_tag="OO369_001413"
     CDS             1413..1913
                     /locus_tag="OO369_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5981742.1"
                     /translation="MQIKSVEDIFIHLLSDTYSAEKQLTKALSKLSRSAYSDKLTAAF
                     QSHLDETHGQIERIDQVVDSEDGLKLKRIKCAAMEGLIEEANEVIESTDKNEVRDAAL
                     IAAAQKVEHYEIASYGTLVTLAEQLGYKKAAKLLKETLEEEKATDVKLTDLAFNNVNK
                     KAQDNS"
     gene            1950..2456
                     /locus_tag="OO369_001414"
     CDS             1950..2456
                     /locus_tag="OO369_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5981743.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAEKMLESMASRIENYPELRSRIEQ
                     HISETKNQLSQLESILDRNNISRSVIKDSMSKMAAFGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASVPIIEAILNEEKQMAEWLLNHIPDTTEQFMVRSE
                     IDGVEAKK"
     gene            2475..3365
                     /locus_tag="OO369_001415"
     CDS             2475..3365
                     /locus_tag="OO369_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HCR5981744.1"
                     /translation="MFRHVKKLQYTVRVSEPNPGLANLLLEQFGGPQGELAAACRYFT
                     QGLSDEDAGRRDMLMDIATEELSHLEIIGSLVGMLNKGAKGALAEGTENEAELYRSLT
                     QNGNDSHITSLLYGGGPALTNSAGVPWTAAYVDTIGEVTADLRSNIAAEARAKIIYER
                     LINVTDDPGVKDTLAFLMTREAAHMLSFEKALHSIRNTFPPGKLPPIEKYKNVYYNMS
                     EGEDVRGSWNSDENFDYVSDPVPAVDGGDGKASINLSTKQEAMIKAMATRLKSHEDIN
                     PVTGAELAEGEPQTKINSKN"
     gene            complement(3485..4066)
                     /locus_tag="OO369_001416"
     CDS             complement(3485..4066)
                     /locus_tag="OO369_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5981745.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTY
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPVIPEV"
     gene            complement(4066..7419)
                     /locus_tag="OO369_001417"
     CDS             complement(4066..7419)
                     /locus_tag="OO369_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5981746.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHAADAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNASASQQSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASSSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASVSATAAGKSAESAASSA
                     STATTKAGEAAEQASAAARSASAAMTSETNAKASETSAESSKMAAASSASSAASSASS
                     ASASKDEASRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGAD
                     IPDKGRFLNNINAVSKTDFADKRGMRYVRVNAPAGATSGKYYPVVVMRSAGSVSELAS
                     RVIITTATRTAGDPMNNCEFNGFVMPGGWTDRRRYAYGMFWQYQNNERAIHSIMMSNK
                     GDDLRSVFYVDGAAFPVFAFIEDGLSISAPGADLVVNDTTYKFGATNPATECIAADVI
                     LDFKSGRGFYESHSLIVNDNLSCKKLFATDEIVARGGNQIRMIGGEYGALWRNDGAKT
                     YLLLTNQGDVYGGWNTLRPFAIDNATGELVIGTKLSASLNGNALTATKLQTPRLVSGV
                     EFDGSKDITLTAAHVAAFARRATDTYADADGGVPWNAESGAYNVTRSGDSYILVNFYT
                     GVGSCRTLQMKAHYRNGGLFYRSSRDGYGFEEDWAEVYTSKNLPPESYPVGAPIPWPS
                     DTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGI
                     KSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSVSGTAASAGNHTHSVTGASA
                     VSQWSQNGSVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHTHSVAIGS
                     HGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            complement(7484..8083)
                     /locus_tag="OO369_001418"
     CDS             complement(7484..8083)
                     /locus_tag="OO369_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5981747.1"
                     /translation="MRKLYAAILFAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            complement(8150..11548)
                     /locus_tag="OO369_001419"
     CDS             complement(8150..11548)
                     /locus_tag="OO369_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5981748.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSDGNTNIAGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     AITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNCSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVMGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGRYTLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRITDIRQVETTARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVELTEDNASRLEEFSKEWKDAN
                     DKWNAMWAVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGNVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTNSYASGTITVRISDDQKFDRQV
                     MIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMP
                     AGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            complement(11609..12241)
                     /locus_tag="OO369_001420"
     CDS             complement(11609..12241)
                     /locus_tag="OO369_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR5981749.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDVSTSGLTAQLHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAITGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     TYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(12178..12921)
                     /locus_tag="OO369_001421"
     CDS             complement(12178..12921)
                     /locus_tag="OO369_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5981750.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRNGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLVAASTFV"
     gene            complement(12927..13625)
                     /locus_tag="OO369_001422"
     CDS             complement(12927..13625)
                     /locus_tag="OO369_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5981751.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSGADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(13625..13954)
                     /locus_tag="OO369_001423"
     CDS             complement(13625..13954)
                     /locus_tag="OO369_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5981752.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPRWEATALESFLAEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(13951..16530)
                     /locus_tag="OO369_001424"
     CDS             complement(13951..16530)
                     /locus_tag="OO369_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5981753.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTETDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRILALSRAGQAAGLTFNQTSESLTALVNAGVRGGEQFESISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDSVLDIGRPDTAQGMLEKAEKAFDE
                     ADKKWQWYQSRSHRRGKTSAFLANLRGAWEDRANAQLGLSAATLQADLEKAREMAAKD
                     WAESEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYEATLKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDL
                     WKAESQFAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQ
                     QADKFAQQQRAKRAAIEAKNRGLTDRQAAREATEQRLKEQYGDNSLALNNVMSEQKKT
                     WAAEDQLRGSWMAGLRSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQN
                     WRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFA
                     TGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQ
                     MRRAEGINFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQAQMRDGGLFSG
                     GGR"
     gene            complement(16523..16957)
                     /locus_tag="OO369_001425"
     CDS             complement(16523..16957)
                     /locus_tag="OO369_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR5981754.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            complement(16939..17361)
                     /locus_tag="OO369_001426"
     CDS             complement(16939..17361)
                     /locus_tag="OO369_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR5981755.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGACVVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            complement(17377..18117)
                     /locus_tag="OO369_001427"
     CDS             complement(17377..18117)
                     /locus_tag="OO369_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5981756.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGNGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDHSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEINVTAS"
     gene            complement(18125..18520)
                     /locus_tag="OO369_001428"
     CDS             complement(18125..18520)
                     /locus_tag="OO369_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5981757.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEADFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            complement(18517..19095)
                     /locus_tag="OO369_001429"
     CDS             complement(18517..19095)
                     /locus_tag="OO369_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5981758.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIRVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(19107..19460)
                     /locus_tag="OO369_001430"
     CDS             complement(19107..19460)
                     /locus_tag="OO369_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HCR5981759.1"
                     /translation="MADSDNLFDAAIARADETIRGYMGTSATMTSGELSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWVDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(19472..19867)
                     /locus_tag="OO369_001431"
     CDS             complement(19472..19867)
                     /locus_tag="OO369_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5981760.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELVLRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVIDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTEHGLARMQ"
     gene            complement(19909..20934)
                     /locus_tag="OO369_001432"
     CDS             complement(19909..20934)
                     /locus_tag="OO369_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR5981761.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSNSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            complement(20990..21322)
                     /locus_tag="OO369_001433"
     CDS             complement(20990..21322)
                     /locus_tag="OO369_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR5981762.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGTAVGILAVDADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(21332..22651)
                     /locus_tag="OO369_001434"
     CDS             complement(21332..22651)
                     /locus_tag="OO369_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR5981763.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAQEALATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            complement(22632..24233)
                     /locus_tag="OO369_001435"
     CDS             complement(22632..24233)
                     /locus_tag="OO369_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5981764.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIARRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            complement(24230..24436)
                     /locus_tag="OO369_001436"
     CDS             complement(24230..24436)
                     /locus_tag="OO369_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HCR5981765.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            complement(24433..26358)
                     /locus_tag="OO369_001437"
     CDS             complement(24433..26358)
                     /locus_tag="OO369_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR5981766.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            complement(26333..26878)
                     /locus_tag="OO369_001438"
     CDS             complement(26333..26878)
                     /locus_tag="OO369_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCR5981767.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            27267..27461
                     /locus_tag="OO369_001439"
     CDS             27267..27461
                     /locus_tag="OO369_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HCR5981768.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIKSDEE"
     gene            complement(27626..27832)
                     /locus_tag="OO369_001440"
     CDS             complement(27626..27832)
                     /locus_tag="OO369_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981769.1"
                     /translation="MNKEQSADDPSVDLIRVKNMLNSTISMSYPDVVIACIEHKVSLE
                     AFRAIEAALVKHDNNMKDYSLVVD"
     gene            28118..28528
                     /locus_tag="OO369_001441"
     CDS             28118..28528
                     /locus_tag="OO369_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HCR5981770.1"
                     /translation="MAQVAIFKEIFDQVRKDLNCELFYSELKRHNVSHYIYYLATDNI
                     HIVLENDNTVLIKGLKKVVNVKFSRNTHLIETSYDRLKSREITFQQYRENLAKAGVFR
                     WITNIHEHKRYYYTFDNSLLFTESIQNTTQIFPR"
     gene            28819..29112
                     /gene="bor"
                     /locus_tag="OO369_001442"
     CDS             28819..29112
                     /gene="bor"
                     /locus_tag="OO369_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HCR5981771.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKQTAVAPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSK"
     gene            complement(29144..29605)
                     /gene="rzpD"
                     /locus_tag="OO369_001443"
     CDS             complement(29144..29605)
                     /gene="rzpD"
                     /locus_tag="OO369_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage endopeptidase RzpD"
                     /protein_id="HCR5981772.1"
                     /translation="MSRVTAIISALIICIIVSLSWAVNHYRDNAIAYKVQRDKNAREL
                     KLANAAITDMQMRQRDVAALDAKYTKELADAKAENDALRDDVAAGRRRLHIKAVCQSV
                     REATTASGVDNAASPRLADTAERDYFTLRERLITMQKQLEGTQKYINEQCR"
     gene            complement(29602..30099)
                     /gene="rrrD"
                     /locus_tag="OO369_001444"
     CDS             complement(29602..30099)
                     /gene="rrrD"
                     /locus_tag="OO369_001444"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HCR5981773.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(30099..30314)
                     /gene="essD"
                     /locus_tag="OO369_001445"
     CDS             complement(30099..30314)
                     /gene="essD"
                     /locus_tag="OO369_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HCR5981774.1"
                     /translation="MKFMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            30903..32000
                     /gene="ompC"
                     /locus_tag="OO369_001446"
     CDS             30903..32000
                     /gene="ompC"
                     /locus_tag="OO369_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5981775.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRSDFDNYTEGNGDGFGFSATYEYEGFGIGATYAKSDRT
                     DTQVNAGKVLPEVFASGKNAEVWAAGLKYDANNIYLATTYSETQNMTVFADHFVANKA
                     QNFEAVAQYQFDFGLRPSVAYLQSKGKDLGVWGDQDLVKYVDVGATYYFNKNMSTFVD
                     YKINLLDKNDFTKALGVSTDDIVAVGLVYQF"
     gene            complement(32190..32573)
                     /gene="quuD"
                     /locus_tag="OO369_001447"
     CDS             complement(32190..32573)
                     /gene="quuD"
                     /locus_tag="OO369_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HCR5981776.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(32591..33580)
                     /locus_tag="OO369_001448"
     CDS             complement(32591..33580)
                     /locus_tag="OO369_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5981777.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPEHYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCAHWVLSVVRRDLDFNDNHAV
                     TMPELCWWLVRNDLADALPESAARKALRLPKSVLPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEQYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            complement(33588..34385)
                     /locus_tag="OO369_001449"
     CDS             complement(33588..34385)
                     /locus_tag="OO369_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCR5981778.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSLDKHKPAFWLRNE
                     QTERLISELQICNSVNIEPVNVIRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSASEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRTAINN
                     IKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            complement(34405..34794)
                     /locus_tag="OO369_001450"
     CDS             complement(34405..34794)
                     /locus_tag="OO369_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5981779.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACTAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKTAGAAA"
     gene            complement(34791..35117)
                     /locus_tag="OO369_001451"
     CDS             complement(34791..35117)
                     /locus_tag="OO369_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCR5981780.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEVAGIIRALLAGEENARLRAAHWLH
                     ERGLKV"
     gene            complement(35117..35611)
                     /locus_tag="OO369_001452"
     CDS             complement(35117..35611)
                     /locus_tag="OO369_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5981781.1"
                     /translation="MIMSLLNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSSSKLR
                     QSGRVAHRCEGDTRRHFPRLTEIPQEPEPQPVRESRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQQERNNFLARRELCLRKSSKRAVSGEEWYLSGN
                     YVGA"
     gene            complement(35608..36549)
                     /locus_tag="OO369_001453"
     CDS             complement(35608..36549)
                     /locus_tag="OO369_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5981782.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRRARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKTSRPDASQP
                     DTQTAEQDFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEHAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKREDPYFKASYDNVDYSQIPAGFRG"
     gene            complement(36539..36718)
                     /locus_tag="OO369_001454"
     CDS             complement(36539..36718)
                     /locus_tag="OO369_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5981783.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            complement(36894..37445)
                     /locus_tag="OO369_001455"
     CDS             complement(36894..37445)
                     /locus_tag="OO369_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981784.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCVSE
                     SSDARECAAPGAVACRDCGETNA"
     gene            complement(37438..37698)
                     /locus_tag="OO369_001456"
     CDS             complement(37438..37698)
                     /locus_tag="OO369_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5981785.1"
                     /translation="MQSPLRNVRKAHGFTLQHVAAGVQVNPATLSRIERLEQIPSIEL
                     AERLANFFKGEVSEMQILYPARFQSSQNQNGFKPQEQEVNRG"
     gene            37796..38488
                     /locus_tag="OO369_001457"
     CDS             37796..38488
                     /locus_tag="OO369_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5981786.1"
                     /translation="MNIGNRVRQLRQAKNMKIADLAEAIGVDAANISRLETGKQKQFT
                     EQTLSNIAKSLGVDIADLFTSAHKSNTVYKNSNNEDVAQVKDVFRIEMLDISASAGNG
                     LIQGGDVIDVIHAIEYRTDNAVSMFGGRPANHIKVINVRGDSMCPTIEPGDLIFVDVS
                     INQFDGDGIYVFGFDDKIYVKRLQMIPDKLLVISDNQIYREWGITSENEHRFMVFGKV
                     LISQSQTLKRHN"
     gene            complement(38808..39323)
                     /locus_tag="OO369_001458"
     CDS             complement(38808..39323)
                     /locus_tag="OO369_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981787.1"
                     /translation="MIAELTAAMTAIRETAQIAKLMNEAKTQAEVNAAIGELNSKLAS
                     IQRECVSLVELVGSYQEINASLKAKIAEFENFEAQTEGYILNQLESGTFVYSKEVIVN
                     GGSITMHLCPKCFGQKIVSILQPFPVSEDELFHKSRCLHCENKFLMNKNPDYVSPPSI
                     EELSRKLNGNL"
     gene            39468..39779
                     /locus_tag="OO369_001459"
                     /pseudo
     CDS             39468..39779
                     /locus_tag="OO369_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            39776..40156
                     /locus_tag="OO369_001460"
     CDS             39776..40156
                     /locus_tag="OO369_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981788.1"
                     /translation="MMRSSKMASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMN
                     TKTKGKVSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTI
                     LQEDQGQLLTLAGEPDGKLRAAGH"
     gene            40222..41046
                     /locus_tag="OO369_001461"
     CDS             40222..41046
                     /locus_tag="OO369_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HCR5981789.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            41174..41710
                     /gene="yfdR"
                     /locus_tag="OO369_001462"
     CDS             41174..41710
                     /gene="yfdR"
                     /locus_tag="OO369_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR5981790.1"
                     /translation="MSFIKTFSGKHFYYDKINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPGHA
                     YGMFMERFNDLSELRKCA"
     gene            41701..42063
                     /locus_tag="OO369_001463"
     CDS             41701..42063
                     /locus_tag="OO369_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981791.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLLRKFDALEAKCAA
                     LENKIIPVSAELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            42063..42368
                     /locus_tag="OO369_001464"
     CDS             42063..42368
                     /locus_tag="OO369_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981792.1"
                     /translation="MTTFTNKELIKEIKERISSLEVRDDIERRAYEIALVSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAVIHFSTMTQQEVK"
     gene            42595..43758
                     /locus_tag="OO369_001465"
     CDS             42595..43758
                     /locus_tag="OO369_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5981793.1"
                     /translation="MSLFRRNEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVRAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHSGIMEDIKKA"
     gene            44241..45194
                     /gene="ompT"
                     /locus_tag="OO369_001466"
     CDS             44241..45194
                     /gene="ompT"
                     /locus_tag="OO369_001466"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HCR5981794.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            45381..46865
                     /locus_tag="OO369_001467"
     CDS             45381..46865
                     /locus_tag="OO369_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5981795.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENIYCPGNSEAEIQ"
     gene            complement(47124..47354)
                     /locus_tag="OO369_001468"
                     /pseudo
     CDS             complement(47124..47354)
                     /locus_tag="OO369_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937477.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT        11749 a        12792 c        11134 g        11750 t
ORIGIN      
        1 aaaaaaaaca cagatgatgg ggctaaaatt tacacaccac ttaccctaaa gctttatgac
       61 tggtgggttt tgggagtatc aaatcgtctt gcatggggat gtcctacaaa ggaacacctt
      121 cttccacact ttctggaaca tttaggtaac aaccatctgg atattggtgt tggaactggg
      181 ttttacctta ctcacgtacc tgagagtagt ctgatatctt taatggattt gaacgaagct
      241 agcctgaacg cggcatctac aagggctggg gaatcaaaaa ttaaacataa aattagccat
      301 gatgtttttg aaccttatcc cgcggcgtta catggtcaat ttgattccat ttccatgttt
      361 taccttcttc actgcctgcc tggaaatata tctacaaaaa gctgtgtaat acgcaatgcg
      421 gcgcaggcct taactgacga tggaactcta tacggagcca caattcttgg cgatggagtt
      481 gtgcacaata gcttcggtca aaaactgatg cgcatttaca atcagaaagg catcttttca
      541 aacacaaaag attccgaaga aggcttaaca catatactct cagagcattt cgagaatgtt
      601 aaaaccaagg ttcaaggtac tgtagtaatg ttttccgctt cagggaaaaa atagcatcca
      661 accgcagcac gttcttgcat acgccgtgtc tgcggtttaa tttttactct caaatattgt
      721 agttaagtac aataatgatt cctgtggcta tatagatgtt tttgtaatta gtccttaaga
      781 taaaatttct ctatttatcc aagaaacaga cttcattagg aagagtcgta aaaacattaa
      841 caactaccgc cgtgtaatta aattattttt tagaatgttc tgattgagaa aaacaacgcc
      901 atacacatca aaagatatag aaatgtaaat ttagatttat taatcttttg agttacaacc
      961 taaactttcc gttaatcacc aagatatcaa tagattatac tcactgagta cacattttta
     1021 caatttctat ttctcacatt aagatgcgca taccaatctc ttatttgaca ctttcttatt
     1081 tgttactaga gttaattggg tgctgacaag gcacatgtta tttacaattt gaaaatcaga
     1141 ggagaaatga tatgaccgaa catcgtggcg gtgcaggtaa tttcgctgaa gatcgacaaa
     1201 aagcttctga cgctggtcgt aaaggcggcc aacatagtgg cggtaatttt aaaaacgatc
     1261 cgcaaagagc atcagaagca gggaaaaaag gtggtcagaa tagccatggt ggcggccgta
     1321 aatctgactc ttaatctatc atcccctaca ttcgtgaata actgaagcta actgcgagcc
     1381 tacgggctcg cagtgaaaac tctggagtga ttatgcaaat taaatctgtt gaagatatct
     1441 tcatccactt attatcggat acatacagcg ctgagaaaca actaacaaag gcactgtcta
     1501 aacttagtcg ctctgcatac agtgataaat taaccgctgc ttttcagtca catcttgatg
     1561 agacacatgg tcaaattgaa cgcatcgatc aggttgttga ttctgaagat ggtcttaaat
     1621 tgaaaagaat taagtgtgcc gcaatggaag gacttatcga agaggcgaat gaggtaattg
     1681 aaagcactga taaaaatgaa gtacgtgatg cagcattgat tgctgcagca caaaaagtag
     1741 agcactatga aattgccagt tatggcactt tggtaacact cgctgagcaa ttaggatata
     1801 aaaaagcggc taaactcctg aaagaaacac tggaagagga aaaagctact gatgtcaaat
     1861 taacagatct ggcatttaat aacgttaata aaaaagctca ggataactcc tgaactatag
     1921 acttcaattt cttttaggat aaacagatta tgaatagaat tgaacattac catgactggc
     1981 ttcgtgatgc acatgcgatg gagaaacaag ctgaaaaaat gcttgagtct atggccagcc
     2041 ggatagaaaa ctatccagag ttacgttcca gaattgagca gcacattagc gaaacaaaga
     2101 accagctttc acagcttgaa agcatacttg atcgtaataa tatttcgcgc tctgtcatta
     2161 aagactctat gagtaaaatg gccgcgtttg ggcaatccat tggtggaatt tttccatctg
     2221 atgaaattgt caaaggttcg atcagtggct atgtgttcga acaatttgaa attgcgtgtt
     2281 acacatctct tctcgcagca gccaaaaatg cgggtgatac agcttcagta cccattattg
     2341 aagcaattct taatgaagaa aaacaaatgg ctgaatggct tcttaaccat attcctgaca
     2401 ccactgagca attcatggtc agatctgaaa ttgatggagt tgaagccaaa aaataattct
     2461 tttgaggagg cattatgttt agacacgtga aaaaacttca atatacagta cgtgtaagtg
     2521 agccaaaccc aggcttagct aatctacttt tagaacaatt cggcgggcca cagggtgagc
     2581 ttgcagcagc atgccgatac ttcactcaag gcttaagtga tgaagatgca ggccgaaggg
     2641 atatgctgat ggacatagca acagaagaac taagtcatct cgaaattatt ggttctcttg
     2701 ttggtatgct caacaaaggt gcaaaaggag cacttgctga aggaactgag aatgaagctg
     2761 agctttaccg ttccttaact caaaatggca atgacagcca tataacttct ctcctctacg
     2821 gaggaggccc agcattaact aattcagctg gagtaccctg gactgcagct tatgttgata
     2881 ctattggcga agtcacggcc gatcttcgtt caaatattgc agctgaagct cgggcaaaga
     2941 tcatttacga acgtctcatt aatgttactg atgatcctgg agtaaaagac actctggcat
     3001 ttttaatgac cagggaagca gcacacatgc tttcatttga aaaagcatta cattcaattc
     3061 gtaacacctt tccaccagga aaattaccac caattgagaa atacaaaaac gtatattaca
     3121 acatgtcaga aggtgaagac gtcagaggta gttggaacag tgatgaaaac tttgactatg
     3181 tttccgatcc agttccagcg gttgacggtg gggatgggaa ggcctcgata aatctttcga
     3241 ccaagcaaga agctatgata aaagctatgg caacaaggtt gaaatcccat gaagacatca
     3301 atcctgtcac tggcgctgag cttgctgaag gagaaccaca aacaaagata aatagtaaaa
     3361 actaattatc ttctggtatt tagctatccc tcataagtta attaccgctg agatatccta
     3421 agctctagcc tctgaaaata catttatatt tcgaagcccg cgatatttta agcgggcttt
     3481 tattttaaac ttcaggtatt acaggccact caatatcagg tgcagttgat gtatcaacac
     3541 ggttcagcaa cacccgatac tttttccagg cttccagcaa tgaggtttct tcctccgttg
     3601 cgatttccag atctacagca tcctgaagtg gcgcaatatg ctcactggct acctgcatca
     3661 ggctgttttt tgtttcttcc gcctcccgga tccggaacag tttttctgct tctgcatctt
     3721 tcacccaggc tgtgccgttc cacttctgaa actccccttc cggcgataac caggtaacat
     3781 tttccggtaa tgagccgagt tcagaaataa ataacgcgtc gccggaagcc acgtcataaa
     3841 ccgttttacc ccgatgatct tcaacgagat gccacgatga ctcatcactg ttgaaaacag
     3901 ccacgaagcc agccggaata tctggcggtg caatataggt actgtttgct ggcagacctg
     3961 tatgaggcgg aatatatgcg tcaccttcac caataaattc attagttccg gccagcagat
     4021 tataaatttt tatggtccgt ggttgttcac tcattctgaa tgccattatg caagcctcac
     4081 aatgtagtta aatgcgatgt ttttgacggt gttttccgcg ttaccagcag cgttaacggt
     4141 gatggtgtgt ccatgtgagc caatcgcaac agagtgcgta tgagcaccaa taccgacagt
     4201 atgtgcgtga gcacctgcag atgcggctgt gccgctgaca ctatgagtgt gcgctcctgc
     4261 agcacttgta ttcacactgg ccgcagacac taccttatgt actgacccat tttgtgacca
     4321 ctggctgact gctgatgcgc ctgtgacact atgagtatgg tttccggcac ttgcggctgt
     4381 accgctcaca ctgtgcgtat gcgccccggt gttattcgtg gatttagtgc cgtaatcaaa
     4441 cgacgatgtg gttttcgtcc ccaaatccgt actggatgcg ctggcgctgt gggtgtgcga
     4501 tttaatgccg tcctgttcct gagacaatac ggcacgacca ctggcaggtt tgcccttaat
     4561 cgtccagcca cgcatatcag ggatcacgcc tgacggataa gcggctgcaa gtttcgggta
     4621 tgcagatttg tcaaaagtct gcccctgcat cagggcataa ccagacggaa cggtatctga
     4681 tggccacggg attggtgcac cgactgggta gctttctggt ggaagatttt tcgaggtata
     4741 aacttctgcc cagtcttcct caaaaccata gccatctctt gaggaacggt agaacagacc
     4801 tccatttctg taatgtgcct tcatctgcaa ggtccggcaa cttccgactc cggtatagaa
     4861 gttaaccaga atatagctgt cgccagagcg ggtgacattg taagcgcctg attcggcatt
     4921 ccatggaacg cccccatccg catcggcata cgtatccgtt gcccttctgg caaaagcagc
     4981 cacatgcgcg gcggttaaag taatatcttt ggaaccatca aactcaacac cagaaaccag
     5041 tcttggcgtt tgcagctttg ttgctgttaa tgcattaccg ttcagacttg cggacagttt
     5101 ggttccaata accagttcgc cggttgcgtt atcaatagca aacggtctta atgtattcca
     5161 gccaccataa acatcacctt gattggtaag cagcaggtaa gttttagcgc catcattacg
     5221 ccataatgca ccatactccc cacctatcat tcgaatctga ttaccaccac gcgctacaat
     5281 ttcgtctgtg gcaaaaagtt ttttgcacga caagttatcg ttaacgatta acgaatgaga
     5341 ctcataaaaa ccacgcccac tcttaaaatc aaggataacg tccgccgcga tacattcagt
     5401 cgctggattt gttgccccaa acttataggt cgtatcatta acaacgagat cagcaccagg
     5461 tgcggatatt gacaggccat cttcgataaa cgcaaaaaca gggaaagcag cgccatcaac
     5521 atagaacaca gagcgcaaat catcgccctt attactcatc attattgagt ggatggctcg
     5581 ttcattgttt tgatattgcc agaacattcc ataagcataa cgcctcctgt cagtccagcc
     5641 accaggcata acaaatccgt taaactcgca gttattcatc ggatcgcctg cggttcgcgt
     5701 tgccgtggtg ataatgaccc ttgatgccag ttcgcttact gagccagcag aacgcataac
     5761 aacaacaggg taatattttc cagatgttgc acctgcagga gcgttaaccc gcacataacg
     5821 cataccacgc ttatcagcaa agtctgtttt actgaccgcg ttaatgttgt tcaggaagcg
     5881 tcccttatcg ggtatatcag cgccgttctg gtctttctgc agacgtttct cagcattgtc
     5941 attggctgct tttactgcct ttggcgttgc cgccagcgtt tcagacgtgc tgttggtcgc
     6001 actactgagc tgtactatcc cctttttcgt cgtgctcgca tcctcaagcg ccacggcgga
     6061 tgcaatatcc tctgcccgtt ttgctgctgt ctcggcgcgc gttgccgcgg attccgccgt
     6121 acttttgctc tgagctgccg ccgtcgcact accagctgcc tctgtcgcct tcgtggatgc
     6181 cgtcgtggcg ctgcctttcg ctgctgatgc ctgtctgctc gcctcatctt ttgaagcaga
     6241 cgcagatgat gccgatgacg ccgccgaact ggcggacgat gcagctgcca tttttgagga
     6301 ttctgcactg gtttccgagg ctttcgcgtt cgtttcggat gtcatcgctg cggatgcaga
     6361 cctcgctgct gcgctggcct gttcagcggc ttcgccagcc ttcgttgtgg ctgttgaagc
     6421 ggatgatgcg gcgctttctg ccgattttcc ggctgcggtg gcactgactg aggcctgccc
     6481 ggcacttgtt gacgcggcac tggcagacga cgcagccgct gtttttgagc ctgccgcagc
     6541 tgaggcgctc tgtcccgctg ccgtctcaga agacctggcg ttcgtctcgg acgtttttgc
     6601 cgccttcgcg gaatttcctg ccgccgttgc cgaggaagct gcactactgg cgcttgatga
     6661 tgcgttcgtt tctgatgatt ttgccgcttc ttttgaggcc gccgcatccc gggccgaggt
     6721 cgcagcttct gatgccttcg tggtcgcggt ggatgcagat gtggctgctg attgttgtga
     6781 cgctgaagca ttcgtttctg acgttttcgc cgcaccggca ctggtggccg ccgcgctttt
     6841 tgaggactct gcagcggcag cactttttga tgcttcagtg gcctttgttg atgccgttcc
     6901 tgcgctggaa gacgctgact gagccgacga cgcggcctgt ccggctgacg tgctggctgc
     6961 gcgtgctgag tccgcagcat cagccgcatg ggttgccgcc tcacgggctg atgtgctggc
     7021 atcactggct gacttcttcg cggctgccgt gttctgtgcc accgcggacg cgttacgcgc
     7081 cacctcttcc accatcagtt caaaacggcg cagtgcctcc ggacgggcat catcctccgt
     7141 catggcaccg agaaaatcat tcagcgtacc gggttgagaa tcttcataca cggtgatggt
     7201 cccggcatgt gacggcggga atccttccac caacagaata acgctgtact gaccgtactc
     7261 aacgtccatg ctgtaacgcc cggcttcatc cggattttct gaggccagcg tgttcaccac
     7321 caccgtggtg ctgttacgtt ttgctttcag ctggattgtg cagttctgta ccggttttcc
     7381 tgtgccgtct ttcagtacac ctgaaatctt tactgccata ttcaccccac aaaaaagccc
     7441 gcctgaaccg gcgggctgtc ataacactgt gttacctggc taatcagaac ttataaccga
     7501 cacccacgat gaaaccgtca gtgcgccagt cgccactgcc ggagccttca taagcaatat
     7561 caatggccac ggattcggtc gggttaaact gcacgccagc tccccacgcc agagacgtgt
     7621 tgctgtggcg accgtcatca cttccggtca gtacatcgtg cgttttcccc ttgttgtcag
     7681 ttacgcgaag ataatccccg gagaaagtcg acacacggct gtaagccaca cccgccatcg
     7741 catacgcgct gaaccattca ttcacgcgca cagacggccc cgccatcacg ctgaaccagc
     7801 ggttacgcac ggaatcttca tgccagcggg tatcgctgta atgggtcagc tggcgattct
     7861 tgtctcctgc atagctgaat gacgtcacca tccccagtgt gtccgtaaac tcataacggt
     7921 atttcacgtt aatcccgttc agatcatcgc tgccgggaac gttcgtcgag gcatgaagat
     7981 accccgcgct cagcgtggac tgatgttcag acgcccatgc aggcgcaccg gatacggcca
     8041 gacaaatggc tgcgaacaaa atagcggcat aaagtttacg cataattacc tctcgctttt
     8101 ctgcaataaa aaaggcgtca tttctgacgc ccgttatggg ttataaaatt cagctgatac
     8161 tgatacctgc tgtggatttc ttcatcacca caaccagcag atcgctgata ctggttgttg
     8221 gtgtccagtt attcgctcct gatgaagata cggtgaatgt cagtgtcagt gtcccctgtc
     8281 cggcaggcat atctataact gaggaaaata cgccctgagc atccgtcgtg gactgattaa
     8341 aaatctcctg accattgcgg gtcactctta accggcaggt tgaataccag tatgactgtt
     8401 ggttattact gttgaaattc tcatgcttac caccgcggaa taacactggc ggtatcatga
     8461 cctgccggtc aaatttctga tcatcactga ttcttaccgt gatggtgcca ctggcataac
     8521 tgttcgtgcg ggggaaagac ttgctgaccg ttttgacaat atcgccttca atctgattgg
     8581 ctgacagttt ccccttaatc tgacagtttt cattaatcgt gacattgttg agcgtccctg
     8641 aatttgcatt cacattaccg ctgatatccg catttttagc ggtcagtttt ccgtccggtg
     8701 tcagggaaaa tgccggcgga tttccaccgc tggtaatggt gggagccgtc aggcgtttca
     8761 ggaacacgtc gttcatgaat atctggttgc cctgcgccac aaacatcggc gtttcattcc
     8821 cgtttgccgg gtcaataaac gcgatacggt tagcggcaac cagaaactgg ctcagtttgc
     8881 cttcctccgt gtcctccatg ctgaggccaa tacccgcgac ataatgtttg ccgtctttgg
     8941 tctgctcaat tttgacagcc cacatggcat tccacttatc gttggcgtcc ttccactctt
     9001 tcgaaaactc ctccagtctg ctggcgttat cctccgtcag ctcgactttt tccagcagct
     9061 ccttgccgag atgggattcg gttatcttgc ctttgaaaaa atccaggtaa ccttccgcat
     9121 catcgctcgc ccgaccgacg gcctccacga atgccgattt gccaacggtg ttcacactgc
     9181 gaacgtaaaa ataataatca tggcccggcc tgatattgat actggcagct atccagtaca
     9241 gcgccgtgcc aagatagcgg gctgtggttt caacctgcct gatatcggta atccgcgttt
     9301 ccgagaacca gaactcaaac tgtaccgtcg gatcgtaaac cgcaagatgc ggcgtggcgg
     9361 ttatctgaaa atagcccggc gtcagctcaa tccgcgacgg cgctgccggt gcggcaatcc
     9421 ggaacgatac cgacgccgga tcgccctgct gtccccacgc atttaccgcc cggactgtca
     9481 gcgtgtaccg ccccagcgcc agttgcgtga agcggtatgt ggtttccgtc gtccgggccg
     9541 tgctgaccag ccgctcactg ccgtcatccg ctgccacggt caggcgaagc ataaagctca
     9601 cccccatcac caccttcggc gtgtcccagc gcgccagcac ctgatattcc ccgctgtctg
     9661 cggtgacttc ggcagtcagg tgctgcaccg ctggcggcgt gacaccattc accgtgccgc
     9721 tctggtcgcc gtcaaagtgc gccccgttat ccacgatggc ctctttttcc ggtacatgct
     9781 gcacggcggt gatggcatac gtgccgtcat cgttctcacg gatactcacg cagcggaaca
     9841 ggcgctggcg cagcgtcggc agcttcagcc cccatacgct gtatccggca acgccgtcag
     9901 gaacacggct cacttttacc ttcacgccgt cggtgacgga ctggacctcc acgctgaccg
     9961 gattgccact tccgtcaacc aggcttatca gcgtggtacc ggaggatggc agcgtgattt
    10021 cacggtcgag cgtcagcgtc cgggtctggc tgtttaccgc cagcacgcgc ccgccggtgc
    10081 tgataccggc atagtcatca tcgcagattt caatgacatc gcccggtaca tggcgaagcc
    10141 cttcggcacc cacgctgaag tccacggtct gcgtctccag cagctccgtt ttaatcagcc
    10201 acagccctgc gcggtgtgcc tgcccccggc tggtacagcc aaaggcatcc atcttcgtga
    10261 cgttacgacc gtaacgggca atggcctgcg tgtcctccac aagctctgtc gccgtctccc
    10321 agccgttatc cgggtcaatc cagttcacct caacggcatt atgacggtcc ttcagggcac
    10381 tgaagctgta gcggaacggc gcgccatcat ccggcatcac cacattactg cagttatagg
    10441 tccacacctt atccgacggt cggtcctgta cgaacgtcag cgtctgcccg ttccataccg
    10501 gcatacagcg catcgccgag cagaaatcac tgagaacatc ccacgccttg cgctgtgtgg
    10561 tcagccaggc attacaggtg atgcgcggct ccgtgccacc aaagccatcc ggcaccgact
    10621 ggtcgcaatt ctggccgatg acatacagcg cccatttgtc cacatccgcc gcaccgagac
    10681 gcttccccat gccgtagcgc ggatgggtca gcatatccca cagacaccag gccatgttgt
    10741 tgctgtatgc tggcttaaac gttccgtccc agataccgct gtattgccgc gtctgcgggt
    10801 tatagttcga cggcacctgc agaatgcgcc cgcgaagatg ataattacgg ctcacctgct
    10861 ggctgccgaa ctgttccgag tccacctgca cgccgaccag tgccgtgttc gggtagcact
    10921 gtttcacatc gatgatttcg gtgtatgacg accagagcgt tttgttctgc agctggtctg
    10981 tggtgctgtc cggcgtcatt ctgcgcatcc ggatattaaa cgggcgcggc ggcaggttat
    11041 ccaccaccac cgaggccaga tactgcgagg tggttttgcc cttaatggtg atgtcttttt
    11101 ccgtcaccca gccaccatta cgttgtatct gaaccagcag gcgaacttcc gacggattcc
    11161 ggtccccctt tgaggtggtt tccaccagtg cctgcacacc gaaggtaaag cgcagtcggt
    11221 cgatgtttgc cgacgtgatg gcgcgggtga tcggcgtgtc gtatttcact tccgtaccca
    11281 acaccgtctc ggagccggag gattcaaatc cctccggcgg agtctgctcc tgctcaccag
    11341 cccggaacac caccgtgaca ccggcgatgt tggtattccc atcactgtcc agcaccggcg
    11401 tactgttcag cagcacgctt tttaatccat ccaccggacc ttcaatcggc ccttcactga
    11461 tggcatcaat cacactcagc aactgcgtgg acttcaggtt gtccttcgct tcgcgcgggg
    11521 tatgcccctt actgcttcct ttacccattc ctcacgctcc ataaacgaca aaaccgcccg
    11581 caggcggttt cacataaaac attttgcatc agcgaccaat caccacaacc tgaccaccgt
    11641 ccccttcgtc tgccgtgctg atctcctgag aaaccacgcg tgaccccacg cgcatttccc
    11701 cgtacagaac aggcagaaca ttgccctggg caaccatgtt atccagtgag gagaaatagg
    11761 tgttctgctt accgttatcc gttgtctgtg tacggggagt tctggctttc ggtgccagca
    11821 tctgcgccac accaccgagc accatactgg caccgagaga aaacaggatg ccggtcatac
    11881 caccggcccc aatggctgcc ccccatgctg caagggtggc tccggcggta aagaatgatc
    11941 cggtaatggc ggcagccccc aggacaatct ggaatacgcc acctgacttg gccccggcga
    12001 ctctgggaac aatatgaatc acagcgccat caggcagagt ctcatgtaac tgcgccgtta
    12061 acccggacgt gctgacgtcc cgcccggcaa tccgtacctg ataccagccg tcgctcagtt
    12121 tctgacgaaa cgccgggagc tgtgtggcca gtgcccggat ggcttcagcc cccgttttca
    12181 cacgaaggtc gatgcggcga ccaaatcgtt gtaaatcccc gtaaaggcag atgcgcgcca
    12241 tgcccggtga cgccagaggg agtgtgtgcg tcgctgccat ttgtcggtgt acctctctcg
    12301 tttgctcagt tgttcaggaa tatggtgcag cagctcgccg tcgccgcagt aaattgcggc
    12361 gtgattcggc accgatgaac caaaacagca cagcagcaca tcgcccggct gtgccgctga
    12421 caacggcacc tgatacagcc ccgtcgcctc cagattatcc agatagagat tctggccgtt
    12481 acgccaccag tcatcctcac gatgaaagtc cggcatctca atccccgcca gatgataagc
    12541 atcccggaac agtgtgtaac agtccgtcac accgtgctca aagcgccgcc cggtgagatg
    12601 cggcacacag cggaacttat gaatcgtccc ccggcagacc agccaccacg gcaaatcact
    12661 ctgcacctgc agccgccggt cggcctcact cagccagggc agaccaccgg ggtggctgtg
    12721 gaccagcgcc acaatctcac cctgcatttc tgcctgcagc cagtcttccg gcgacatacg
    12781 gaaatacgcc tccggctcac cggagatatt cacgcagggg aaatatcttt ccccctccgg
    12841 cgtgcttacc acgaagccgc acgactccgc tggcgcacat cgccgggcgt gcgccagaat
    12901 cgctgattct gtctgtgtca tgggatttac tgcgaaagtt tgttaatgga aaggaagccg
    12961 ccaaagttgc cgacgttatt gcggaactta caaccgctca ggcatttgct gcatttatcc
    13021 ttcgtgatat cggacgttgg ctggtcatat tcatccgcga cagccggacc gctataaccg
    13081 cactcgtcac cgcgataggt ccaggtgcag gtgttggcca gcatgatacg tcccggaaaa
    13141 acagcgccat ccgtttccgt cggcgtggac agtacaaaag aggcactcac cgcgctcagt
    13201 tcgctgcact gctcaatgcg ccagcggctg atcacctcct gctccggatc ggcgccactg
    13261 tttccgttga cgaagttcac cgcatccaga aaacgggcgt aaaccttacg ccggaccacc
    13321 gttccaccga ccagactctg cagatcttcc gccatcccgg tgaccatacc gtacaggtta
    13381 gaaaccgtca gtgtggggcg cgtactggtg cctttgccat tcagttcaaa accactcccc
    13441 tgaatgggat acggctgata ctgtcgcccc tgccaggtga ccggctcacc tttttcgttc
    13501 tgctcattac agaaaaaata acgttctcca ccgacctctg tcaggtcgat ttcccagagc
    13561 accacgctgg ccgactgctc cgcacgggtg cattcattca gtgtttcctg ccggatatcc
    13621 tgcatcagtt caccacctgt tcaaactctg cgctgaactc aacacgcagc atactgaccc
    13681 gcgacgacca ttttgcgcag gtcaccttta tctgccgcca ctcataaggc ggcgtccaca
    13741 gaaaggcttt ccagcccccg tgctcagcca gaaacgactc cagcgccgtg gcctcccaac
    13801 ggggaacaga aagcgtcacg ctgtacgttt tcaggtcggc attcagcccg gcaggcgctc
    13861 gctgggaata gccatcacca aagcgcactt tcctgacgga aggggccgaa gccacatcca
    13921 taccgggttt cactttccag cggaaggtct tcatcgtcca cctccggaga acagaccacc
    13981 atcgcgcatc tgtgcctgaa tttcatcacg ggcacccttg cgggccatgt catacactgc
    14041 cttcatcatc tgtggacctg gcagaccatt cgtaccgtcg ttctgaatca ccacgtgatt
    14101 gttctgatta aaattaatgc cttcagcccg ccgcatctgc gccggacttc cggcaccgcc
    14161 cacataacca ccttccgcat agccccgcat caggcggtac aggttgccga caccaattcg
    14221 gctggttgcc tccttcgtga agacaaattc accacggtga acaatccccg ctggctcata
    14281 tttgccgccg gttcccgtaa atcctccggt tgcaaaatgg aatttcgccg cagctgcctg
    14341 aatggctgta ccgcctgatg cggatgcgcc gccaccaaca gccccgccaa tggcgctacc
    14401 gatactcccg acaatcccca ccattgcctg cttaagcaga atttctgtca tcatggacag
    14461 cacggaacgg gtgaagctgc gccagttctg ctcactgccg gtcagcatcg ccgccatatt
    14521 ctgcgcaata ccatcaaagg tctgcgtggc tgcactttta acctgcgaca tactgtccgt
    14581 ggcgctctct tcccactcac tccagccgga cctgaggcct gccatccagc tcccgcgaag
    14641 ctggtcttca gccgcccagg tctttttctg ctctgacatg acgttattca gcgccagcga
    14701 attatcgcca tactgttcct tcaggcgctg ttctgtggct tcccgcgctg cctgccggtc
    14761 agtaagcccc cggtttttcg cctcaatggc tgcccgtttt gcccgttgtt gctgtgcgaa
    14821 cttatccgcc tgctgcgcca gcgcgttcag gcgctcctga tacgtaacct tgtcgccaag
    14881 tgcagccagc tggcgtttgt actccagcgt ctcatcttta tgcgccagca gggatttctc
    14941 ctgtgcggac agctggcgac gttgcgccgc ctcctccagt accgcgaact gactctccgc
    15001 cttccacaaa tcccggcgct gctggctgat tttctcattt gctccggcat gcttctccag
    15061 cgtccggagt tcagcctgaa gcgtcagcag ggcagcatga gcactgtctt cctgacgatc
    15121 gcccgcagac actttcacgc cggactgttt cggctttttc agcgtcgctt cataatcctt
    15181 tttcgccgcc gccatcagcg tgttgtaatc tgcctgcagg attttcccgt ctttcagtgc
    15241 cttgttcagt tcttcctgac gggcggtata tttctccagc ggcgtctgca ggcgttcgta
    15301 agccttctgc gcctcttcgg tatatttcag ccgtgatgcc tcagactcgg cccagtcctt
    15361 tgctgccatc tctctggcct tttcaagatc ggcctgcaac gtggcggctg aaagcccaag
    15421 ttgcgcattc gctctgtcct cccatgctcc ccggagattg gcaagaaatg ctgaggtttt
    15481 accgcgccgg tggcttcggc tctgatacca ctgccatttt ttgtccgcct catcaaaagc
    15541 cttttctgct ttctccagca ttccctgggc agtgtccggg cgaccaatat ccagcaccga
    15601 atcccacatg gatttgaatg cccgtgctgt cctgtctgcc caggtctcca gcgtgcccat
    15661 gttctctttc aggcggcggg tctggtcatc aaaccctttc gttgcggcct cgttcgccgc
    15721 ctgcaatgcc ccggcttcat ctccggaacg ctgcaactga gcaacatacg caatctgctc
    15781 cgccgtcacg ttatggaact gacgtgccat cgctgtcagt cccgacgtcg ggtctgtggt
    15841 cagcttcccg aaggcttcag cgaccttgtc cacctccacg ccggatgcag aggagaaacg
    15901 cgccacactc tggctgatcg actcaaactg ctcaccacca cgcacaccgg cattcaccag
    15961 cgccgtcagt gactcgctgg tctggttaaa cgtcagccct gccgcctgcc cggctctgga
    16021 cagggccaga atacgatctg ccgtcagtcc cgcctgattg ccggaaagga ccagcgtttt
    16081 gttgaaatcg gacagggttg agttgccctg ataccaggca tacgccagcg caccggtcgc
    16141 caccgccagc gaggtggccc ccaccatcgg cagggtgatc gcaccggcaa gccccctgaa
    16201 catggggatc atcccgccga aggagtcctt aacctgacca ccctgttgca gcaggatcag
    16261 ccacggactt tgcccgcctg caagctgcgt ggccacgtcg gtgaactgtg caggcagcat
    16321 acgcatggcg gctttatact gcccgacgga aatccccgct ttctgtgcag ccagcgcctg
    16381 tcggctcagc gactgttcaa cgactgccgc tgtttttttc gcatcagttt ccgtaccgga
    16441 aaaatgacgc ctgactctgg ccatctgctc gtcaaatctg gccgcatcca gacttaaatc
    16501 aacgaccaga tcgcctaccg gttcagccat accggactcc tcctgcgatc ccttctgata
    16561 ctgtcatcag cattacgtca tcctccgtca tgtccgccac atccggggaa gcggggataa
    16621 cttcattccc gtccgggcca aaacgaacgc ctccggcaag ccctgccgct ttctgcatca
    16681 gcacatcatc ttcaggctct tcgtcagcct cgcgccggtt cagcagactg aaatccagcg
    16741 gatgcatatc cggatcgctg aaaaacaggc tgagcacggt gtacgtcagc ccggaaaagt
    16801 gcatatccag cagaacatca tgaaaataat gggtactgta aaagcggtgc cagtcggcat
    16861 actccgtgga tgacatcccg gcaagcatgg cacgccagtc gggtcgcccc atctcgcgcg
    16921 ccagtttcag ggcaaaactc agctcaccgt cgaacacttt cccgcagaaa caggctctgc
    16981 aggcccggca tcctctgcct gttcaggggc atcattcacc acaaactcat acataccgga
    17041 cagccggtac accacgtttt cagcatgaga aattgcctct gtaggccagg tggtaagcac
    17101 ttcctgctca atctgtttaa cggcttcatt catggaaggc atctgcgtct tctgcggatg
    17161 gttatgccac agggacatcg ccaccacaca agcaccggtt ctgatggcgt cttccacagt
    17221 aaacttccgg ttgctgtctg actccgcctg ttctgcctgc cgtttcatca gggcgagatg
    17281 ctcaatacgc tgcagggctg acagttcaga aagcgtgacg gtcacaccgt tatgttcaaa
    17341 tgattcggtt ttcaggaaca tcgctgactc tccggattaa ctggcggtga cgttgatttc
    17401 tgcaaccgca gcaagttcac cattaccgga tacgaccgga atgttgacct tgcctgcagc
    17461 aacacctttc acggtgatgg tcataccact gaccgacacg gtggcttttg ttttatccgc
    17521 tgacaccgca cggaagctct tgtcggttgc gccttccggc tggaatgcca cggtcagcgt
    17581 ggtgctctgc cctttcacca ccgaggtgct ggcaggcgtc acggtcatgc cggttgccgc
    17641 tgtgaccgtg ctgtgatctt ctgccatcga cggacggccc acattggtga ccttcaccgt
    17701 gcgggtgatc acttccttcg ccgtcaccgc cttaccgata ctgctgaccc agccgcggaa
    17761 cacatcgacc gtgccattcg ggaagcggat tttataggct cgggtatccc cttcattaaa
    17821 ccacgccagc agcgcctgct gcccctgctc tccgggcatc cacgccagcg tgaagctggt
    17881 atctccggca gatttctgcc cctgcccggt cgcagtccag tccgcatctt catcatcgag
    17941 atagctgtcg tcataggact cagcggtcag ttcgccgggc gtcaggtctt taactttagc
    18001 cagacgcgac cagtcaacgt ctgaaagcgg gttcgcataa gggtcaccgt tccccttata
    18061 aacccacagt gtggtcccgg cccctttcac cggcattact ggatttggta caggcatagc
    18121 gtcctcacat ttcataggta atgacataag tcagatcggc tgaactccac aggcccgcat
    18181 catcgtcgcg ccggtagtca tagccgctgg ccaccatact ggtgatcaaa tctgacagtg
    18241 ccgggatatc gctcatcacc ggataaatcc gggactccat ccacgaatcc agctctgaat
    18301 ccggcacctg agcaggcagg aaaacttcaa tatgcagctc cgcctgccag gtatcgctgt
    18361 ccagctcttc gcccgtgtat tcagcgccgg tgagataaac ggcaattgcc ggaaaatccg
    18421 cctcatcaaa aacagcgggg cgaccatcaa aaagcgtcgc cccggtgtca tgcttctcca
    18481 gtgcatccag tacggctgca cggagttcag tatgtttcat cgctttatta ccattctcag
    18541 ttgatgctgc agcgcatagc ccagctcttt cggaagacgt tcacgccgta tccgttcaat
    18601 attctgttta aacgccgtgg taagcggcac cgccatcggg attttcacca catcaatggg
    18661 gtaacggttt ttcccggcca cacgctgcat gacatgccac cggccatttt tcagttgctg
    18721 aataaacgcg ccgggaatac gacggtttcc caccacaagc acgctgccgc cacctttcag
    18781 ggatgaacgc tgcccctttt tacgacgtct gcggcgggaa aggacaaccc gcgcgttacc
    18841 cagcttgatt acgggcaaat ccccccggtt aaccctgatt ctggcctgcg gatttttgac
    18901 cgtggccctt ttcagcctgg ccctttcctt taccagtttc cggcgtacct ttgtctcacg
    18961 ggcaacctgt gacgcagact gcgatatcgc ggatgacgca acgcggttaa tggccattgc
    19021 ggcggcacca ggcaccgccg ttttgctgat acggctgagg ttttcaacgg cctgctcaag
    19081 accttttatg gccatacatc cccctttcag cggcgacggt taacggcagg cggcacgcca
    19141 cgcccaagcc agagatgaca gcttccgcca tcatccggcg aaatccggtc cacccagaag
    19201 ttttcctcgc cgatggtcag tgtgtctcca cgccgcagct gccgcacatc atcagtccgg
    19261 acaaacaggg acgggctgga gccttcaacg cgcaccccct gtccggcata gctgatattt
    19321 tcagggtcat caaaaacacc acgtatcaca gcaccggaca gctcaccgga tgtcatggtg
    19381 gctgacgttc ccatgtaccc gcgtatcgtt tcatcggcgc gggcaatggc agcatcgaac
    19441 aggttatcgg aatcagccac agcgcctccc gttattgcat tctggccagg ccatgttctg
    19501 tcatttcggc tgccacaccg gcagagacac gaaacgccgt tcccggcagc acaaatgcca
    19561 caggctcatc ccgcgtggcg tgaagtgcat cagtatgcag cgtcaccagt gccacgaccg
    19621 tgaccagagc agccgtatca atcacggtat ccggctgcgc tgataccacc tcattttcat
    19681 gtccggtcag cgcattttcc gggctgacag atgtgtcctg accggcagcg tcatccgtgt
    19741 catcaagctc ctcttccagc tctgccacac ggagcaccag ttcttctttc gtccccgtca
    19801 ggctgacatc acggttcagt tgctcaccca gcacctgaag acgggcaatc agttcatctt
    19861 tcgtcatgga ctcctccaca gagagaaaat ggccccgaag ggccatgatt acgccagttg
    19921 tacggacacg aacgcatcag gatcagccag cagcatcagc ggtgctgact gaatcatggt
    19981 gaactcacgc gccggatcgc cggtggtcac ccagtttttc gggtagcggg cagaggcgtt
    20041 aataccttcg cgctgtgcgt ccgcatcctg aatgcagcca taggtgcgca gaccgcgtgc
    20101 ctgagtgttc cccagcacca tcgtgttgtc cggcaggaag ttctttttga cgtcgttttc
    20161 cacgtactgt cctgaataca cgacgatcgc cgtatcgcca tacatcccct tataggacac
    20221 cgcttcgccc aggtctttta ccgctgtctc cagctcggaa ttagagccgc gacgggtatc
    20281 cagcttctcc ttgacggctt tgaaggaacg gaacagcgcc cagcctttcg gatcaaacac
    20341 gatgatattc accacgccgc tggcgttcag cgcataggct tcgatatcgt cggtcgggtc
    20401 atacgtggac ttgtcacgct tgctccactc cgtaccaccg gactgtgtga tgttgttcgc
    20461 cgcactgcgg cccatatcca cctcaaccgg atcgaaggct tcaccggtca tggtgtattt
    20521 gcccttaagc acagcagaaa ctgcctgcat ctcttcgacc tgagcaatgg ccagctcttc
    20581 gtctcgcatg ttctgcatga tgatgcgacg gcggcggtaa gccgggtccg ccagattctg
    20641 cggatcttca tccggcaggc gacgcagggt catctgcgga ttcacctcat gcttgggttt
    20701 gacatatccc ggcgtaaatt cagaggtgga gccgccacgg gaacggataa cctcaccgga
    20761 aacaatcggc gaaacgtaca gcgccatgtt taccagtccc ggaatttgtg agagatagac
    20821 tttctccgtg gtgaagggat agctctcacg gaaaaagaga cgcagaaaca gcggatcaaa
    20881 cttaaatttc tgctcatttg ccgccagcag ctgggcggtt gtgtacatcg acataaaaaa
    20941 atcccgtaaa aaaagccgca caggcggcct ttagtgatga agggtaaggt taaacgatgc
    21001 tgattgccgt tccggcaaac gcggtccgtt ttttcgtctc gtcgctggca gcctccggcc
    21061 agagcacatc ctcataacgg aacgtgccgg acttgtagaa cgtcagcgtg gtgctggtct
    21121 ggtcagcatc aaccgccaga atgccaacgg cagtaccgtc ggtggtgcca tcccacgcaa
    21181 ccagcttacg ggtggaggtg tccagcatca gcggggtcat tgcaggcgct ttcgcactca
    21241 atccgccggg cgcggttgcg gtatgagccg ggtcactgtt gcccagcggc tggtaatggg
    21301 taaaggtttc tttgctcgtc ataaacatcc cttacactgg tgtgttcagc aaatcgttaa
    21361 cggcatcaga tgccgggtta cctgcagcca gcggtgccgg tgccccctgc atcagacgat
    21421 ccagcgcagt gtcactgcgc gcctgtgcac tctgtggtgc agctgccaga atgcggcggg
    21481 ccgtttccac ggtcataccg ggggtttctg ccagcacgcg ggcctgttct tcgcgtccgt
    21541 gagcctcctc acagttgagg atccccataa tgcggctgtt ttctgccgca accgcagcgg
    21601 tgatctgcgc gttcacgtcc ggctgcgccg cgctggcgtt ttcgccctcc gtcgctggca
    21661 ccacgtcagt aacgtcagcc tgcgaagcag tggctgaaac agttgttgat tgagtctctt
    21721 tggtcattcg ccctcctgag agacgggatt tacgtgcatc cagtgcatca cgcataacgg
    21781 tgatcgcatc ggtgctgttg acaagttcat cagccagtcc ggcatcaatg gcctcctgac
    21841 cgctgtacac tgcagcctcg gtatccagca cagcctgcac ggacaggccg gtatatgccg
    21901 acaccttctg tgcaaacatc cggcgggttg catccatccg ggactgcaat gtttcccgga
    21961 catcatccgg tagatggctg taggggttgc catccacctt atggctgccg ctgtaaatca
    22021 gcgtgatttc cacgccctgt ttctccagtg cagcgccgta attactgtga gccatcatga
    22081 cgccgatgga gcctgtccgg gcggtctgcg tgaccagacg ccgggaggcg gcactggcaa
    22141 gcagctgacc tgcgctgcag ttcatgtcat tggccagcgc ccataccggc tttatgtcac
    22201 gcacacgggc gatgatgtca gcgcagtcaa atgcccccgc caccatcccg ccgggcgtgt
    22261 ccatatcgag cagaatgccg tccaccatcg gatcgctggc agcctgttgc agacgggcga
    22321 taatgccgtt gtaaccggtc atccccgagt acggctgcag cgcccgcgtc cggctgacca
    22381 gcgtgccgga caccggcagc acggcgatgc cgttcatgac ctgataactg cgggcctgtc
    22441 gtggtccgtc atcatcaccg gataatgcca gcgtcgcgag tgcctcctgg gcagtcaggc
    22501 tgtcgccgga caccgcatcc gtcaggcggc tgatcccaag ctggcctgca agcgcacaaa
    22561 agaaaacccg cgcataggcg ggttcaagca tcagcggctc attaaaagcc atgctggcaa
    22621 tatgcgggag attacgcagc tctgctgtca ctcttctcct cctctgttga ttgtcgcagc
    22681 ccggattcaa atgccgcagc cgcccaggcg ggcggtttaa gaccagctgc gcggcgctcc
    22741 atcgtttcac ggacctgctg ggcaaaaatt tcctgatagt cgtcaccgcg tttcgcgcac
    22801 tctttctcgt aggtactcag tccggcttct atcagcatca ccgcttcctg tacttctttc
    22861 agaccatcga tggccatacg accggagcct atccagtcgc agttccccca ggcacttcgg
    22921 gcttcctgaa agctgaagcg cgcttttgaa ggtaacgtca ccacgcggcg agcgatggcc
    22981 tcttccagcc agcacagaaa catctggctc gcctgacggg atgcgacgaa ttttcgccgc
    23041 cccataaagt acgcccacga ctcgttcgca ctggcccgtg ccgtggagta gctcatctgg
    23101 gcgtaattcc gggaaagctg ctcatacgag acacccagcc cggcagcgat ataccgcaac
    23161 agtgactgct caaacacgga gtagccgtta tccgtgtcct gagccgtctg caggttcagt
    23221 gagtcccccg gcatcaggtg cggcactttt gcgcctccca gccggaccgg tgctgcggcg
    23281 taatacgcgg caatttcacc aatccagccg gtcagccttt cccgctgctc ctgactgttc
    23341 gcgcccagaa taaaatccat cgctgactgc gtatccagct cactctcaat ggtggcggca
    23401 tacatcgcct tcacaatggc gctctgcagc tgcgtgttct gcagcgtgtc gagcatcttc
    23461 atctgctcca tcacgctgta aaacacattt gcaccgcggg tctgcccgtc ctccacgggt
    23521 tcaaaaacgt gaatgaacga ggcgcgtccg ccgggtaact cacggggtat ccatgtccat
    23581 ttctgcggca tccagccagg atagccgtcc tcgctgacgt aatatcccag cgccgcaccg
    23641 ctgtcattaa tctgcacacc ggcacggcag ttccggctgt cgccggtatt gttcgggttg
    23701 ctgatgcgct tcgggctgac catccggaac tgtgtccgga aaagccgcga cgaactggta
    23761 tcccaggtgg cctgaacgaa cagttcaccg ttaaaggcgt gcatggccac accttcccga
    23821 atcatcatgg taaacgtgcg ttttcgctca acgtcaatgc agcagcagtc atcctcggca
    23881 aactctttcc atgccgcttc aacctcgcgg gaaaaggcac gggcgtcttc ctccccgatg
    23941 cccagatagc gccagcttgg gcgatgactg agccggaaaa aagacccgac gatatgatcc
    24001 tgatgcagct ggatggcgtt ggcagcatag ccgttattgc gtaccagatc gtctgcgcgg
    24061 gcattgccac gggtaaagtt gggcagcagg gctgcatcca cactttcacc cggtgggttc
    24121 cacgcccgca actgcccacc aaatccgctg ccaccgccgt gataaccggc atattcacgc
    24181 agcgatgtca tgccgtccgg tcccagaagg gtgggaatgg tgggcgtttt catacataaa
    24241 atcctgcagg tcccctgcgt cgctgtgtca tgccggtctg cacctccagc tccgcaatgt
    24301 attttttcag gtcagacacg gaagtggccg taaactccac tcgccgtccg tctttctgta
    24361 ccgttgccac ccgtttacct gtcatcaggt catgcagtgc cgcacgggca gcggcaagtt
    24421 cttcctgtcg cgtcattcat cctctccgga taaggcacgg gcgtaatctg ccagtgtttt
    24481 cttgttggtt gctgcaccat cttcttcctg caggctcgcc agcagtgcac tgagatccag
    24541 ctgccagcgg gaaatactga tgcgcagcgc cgccagcgca taaacgaagc agtcgagcgc
    24601 ctcattgcgt cgctttttgc tgtcccacag tatttttttc ctgccatcca cccatttttc
    24661 gacctgctct tcagcagtca gctgctgcgc ttcggtcaga tcaaaaatat ccgggttatt
    24721 cgggaagtga acggcaccgg gaagcggttc atccccttcc ggcgtcagtg tgaagcggtt
    24781 ataaatctgc tctttcgcgg tatccgtacc gatttcggta aggtaaaccc cgtttttgtt
    24841 tcgcttacga ggcatgctgg ccaccggctt accgtagacg gatgcccctt taatggggat
    24901 cacccggaac agcccatgct ttttcgagcg ttcatacaca atggtcgggt caatcccgcc
    24961 aatatcccag cagatacggg atatcgacat ttctgcacca ttccggcggg tataggtttt
    25021 attgatggcc tcatccacac gcagcagcgt ctgttcatcg tcgtggcggc ccataataat
    25081 ctgccgatca atcagccagc tttcctcacc cggcccccat ccccatacgc gcatttcgta
    25141 gcgatccagc tgggagtcga taccggcggt caggtaagcc acacggtcag gaacgggcgc
    25201 tgaataatgc tctttccgct ctgccatcac ttcagcatcc ggacgttcgc caattttcgc
    25261 ctcccacgtc tcaccgagcg tggtgtttac gaaggtttta cgttttcccg tatccccttt
    25321 cgttttcatc cagtctttga caatctgcac ccaggtggtg aacgggctgt acgctgtcca
    25381 gatgtgaaag gtcacactgt caggtggctc aatctcttca ccggatgacg aaaaccagag
    25441 aatgccatca cgggtccaga tcccggtctt ttcgcagata taacgggcat cagtaaagtc
    25501 cagctcctgc tggcggatga cgcaggcatt atgctcgcag agataaaaca cgctggaggg
    25561 gtcatccggc gtccatttga ggccaaacgg cgtctctttg tcgccaaatt taagatactg
    25621 ctcctccccg cagtgcgggc aggcaacatg aaaacgcata aaatgcgggg attcactggc
    25681 tgcacgctca atctggcagg tgcctctcac tttgggcgtg gagccacgga tggactttgg
    25741 ccagaccgag ccttcaatac gtttgtcgcc aaggaacgtc ggagagcctt cctgttcaat
    25801 atcctcatca aaggcagcaa gttcatcata acccgccaca tccaccgatt tttcacggta
    25861 gttttttgcc gctttaccac ccaggcacca gaagccacga ccattggaaa aacgcttcat
    25921 agtgagcgtg ttatcccggt gctttttgcc ataccacgga gccagcgcca gcagcgacgg
    25981 aatatcgcgg atggtcggct caacgtgggt tttcataaag ttctcggcat caccatccgt
    26041 cggcaaccag ataagtgtgt tgcgctgctt atgctctata aagtaggcat aaacacccag
    26101 cagcattttg gaataaccga cacgggcaga cttcaccaca ttcacctcac ggatgtagtc
    26161 gctgcccatc gcattcatga tggcccgctg aaagggcagt gtttcccagc gcccttcctg
    26221 gtatgcggat tctttcggga gatagtaatt agcatccgcc cattcaacgg cggtctgtgg
    26281 ctccggcctg aacagtgagc gaagcccggc gcggacaaaa tgccgcagcc tgttaacctg
    26341 actgttcgat atattcactc agcaaccccg gtatcagttc atccagcgcg gctgctttgt
    26401 tcatggcttt gatgatatcc cgtttcagga aatcaacatg tcggttttcc agttccggaa
    26461 aacgccgctg caccgacagg gggatcccgt cgagaatact ggcaatttca cctgcgatcc
    26521 gcgacagcac gaaagtacag aatgcggttt ccaccacttc agcggagtct ctggcatttt
    26581 tcagctcctg tgcgtcggcc tgcgcacgcg taagtcgatg gcgttcgtac tcaatagtcc
    26641 ctggctggag atctgtctcg ctggcctgcc gcagttcttc aacttcccgg cgcagctttt
    26701 cgttctcaat ttcagcatcc ctttcggcat accatcttat aacggcggca gagtcataaa
    26761 gcacctcatt acccttgcca ccgcctcgca gaacgggcat tccctgttcc tgccagttct
    26821 gaatggtacg gatactcgca ccgaaaatgt cagccagctg ctttttgttg acttccattg
    26881 ttcattccac ggccaaaaac agagaaagga aacgacagag gcccaaaagc tcgttttcag
    26941 cacctgtcgt ttcctttctt ttcagggggt attttaaata aaaacattaa gttacgacga
    27001 agaagaacgg aaatgcctta aaccggaaaa ttttcataaa tagcgaaaac ccgcgaggtc
    27061 gccgccccgt aacctgtcgg atcgccggaa aggacccaca aaatgataat aattatcatc
    27121 tacatatcac aacgtgcatc tacgccatca aaccacgtca aataatcaat tatgacgcag
    27181 gtatcgtatt aattgatctg catcaactta acgtaaaaac aacttcagac aatacaaatc
    27241 agcgacactg aatacggggc aacctcatgt caactaagaa cagaacccgc agaacaacaa
    27301 cccgcaacat ccgctttcct aaccaaatga ttgaacaaat taacatcgct cttgagcaaa
    27361 aagggtccgg gaatttctca gcctgggtca ttgaagcctg ccgtcggagg ctaacgtcag
    27421 aaaagagagc atatacatca attaaaagtg atgaagaatg aacatcccgc gttcttccct
    27481 ccgaacagga cgatattgta aattcactta attacgaggg cattgcagta attgagttgc
    27541 agttttacca ctttcctgac agtgacagac tgcgtgttgg ctctgtcaca ggttaagtag
    27601 tttgaatgat tagcagttat ggtgatcagt caaccaccag ggaataatcc ttcatattat
    27661 tatcgtgctt caccaacgct gcctcaattg ccctgaatgc ttccagagac accttatgtt
    27721 ctatacatgc aattacaaca tcagggtaac tcatagaaat ggtgctatta agcatatttt
    27781 ttacacgaat cagatccacg gagggatcat cagcagattg ttctttattc attttgtcgc
    27841 tccatgcgct tgctcttcat ctagcggtta aaatattact tcaaatcttt ctgtatgaag
    27901 gtttgagcac gttggcctta catacatctg tcggttgtat ttccctccag aatgccagca
    27961 ggaccgcact ttgttacgca accaatacta ttaattgaaa acattcctaa tatttgacat
    28021 aaatcatcaa caaaacacaa agaggtcaga ccagattgaa gcgataaaaa cgataatgca
    28081 aactacgcgc cctcgtatca catggaaggt tttaccaatg gctcaggttg ccatttttaa
    28141 agaaatattc gatcaagtgc gaaaagattt aaactgtgaa ttgttttatt ctgaactaaa
    28201 acgtcacaat gtctcacatt atatttacta tctagccaca gataatattc acattgtgtt
    28261 agaaaacgat aacaccgtgt taataaaagg gcttaaaaag gttgtaaatg ttaaattctc
    28321 aagaaatacg catcttatag aaacgtccta tgataggttg aaatcaagag aaatcacatt
    28381 tcagcaatac agggaaaatc ttgctaaagc aggagttttc cgatggatta caaatatcca
    28441 cgaacataaa agatattact atacctttga taattcatta ctatttactg agagcattca
    28501 gaacactaca caaatctttc cacgctaaat cataacgtcc ggtttcttcc gtgtcagcac
    28561 cggggtgttg gcataataca atacatgtac gcgctaaacc ctgtgtgcat cgtttttaat
    28621 tattcccgga cactcccgca gagaagttcc ccgtcagggc tgtggacata gttaatccgg
    28681 gaatacaatg acgattcatc gcacctggca tacattaata aatattaaca atatgaaatt
    28741 tcaactcatt gtttagggtt tgtttaattt tctacacata cgattctgcg aacttcaaaa
    28801 agcatcggga ataacaccat gaaaaaaatg ctactcgcta ctgcgctggc cctgcttatt
    28861 acaggatgtg ctcaacagac atttactgtt caaaacaaac agacagcagt agcaccaaag
    28921 gaaaccatca cccatcattt cttcgtttct ggaattgggc agaagaaaac tgtcgatgca
    28981 gctaaaattt gtggcggcgc agaaaatgtt gttaaaacag aaacccagca aacattcgta
    29041 aatggattgc tcggttttat tactttaggc atttatactc cgctggaagc gcgtgtgtat
    29101 tgctcaaaat aattgcatga gttgcccatc gatatggtca gctctatctg cactgctcat
    29161 taatatactt ctgggttcct tccagttgtt tttgcatagt gatcagcctc tctctgaggg
    29221 tgaaataatc ccgttcagcg gtgtctgcca gtcgggggga ggctgcatta tccacgccgg
    29281 aggccgtggt ggcttcacgc actgactgac agactgcttt gatgtgcaac cgacgacgac
    29341 cagcggcaac atcatcacgc agagcatcat tttcagcttt cgcatcagct aactccttcg
    29401 tgtattttgc atcgagcgca gcaacatcac gctgacgcat ctgcatgtca gtaattgccg
    29461 cgttcgctag cttcagttct ctggcatttt tgtcgcgctg gactttgtag gcgattgcgt
    29521 tatcacggta atgattgacc gcccatgaca ggctgacgat gatgcagata atcagagcgg
    29581 atataatcgc ggttactctg ctcactgttg cccccacaaa cagacttcac gctcaatctc
    29641 acgacgagtc atcaggcctt tccattgctt accgccagcg tatgtccagc gacgcagctg
    29701 atcacatgcg cctttgatat cgccctggtt tattttgcga agaagcgtcg atgttctaaa
    29761 attgccagca cccacgttgt aaacgaatga gtaaagagcg ccgcgcgttg tttccggtat
    29821 atcgactttg atatacgggt taatttgtct ggcgacagtg gcaaggtctt tattcaagag
    29881 tgctttgcat tctgctttgg tatacgtttt accgagcatg atgtcttttc cggtgtgtcc
    29941 gtgacataca gtccatacac caacaatatc tttgtatggt atgtagctga caccttccag
    30001 accatcgtta ccacttgggc cagtgattaa cactgatgct atagcaattg ctccgccacc
    30061 aatagcagca gcaacggctt ttcgtaatga tggaggcatt attcacctct cgcagccttg
    30121 cgcttatctt ctttaatctt gaaataaagg tttgtcaggt acgtcagcag gccaaatacc
    30181 aggctaccca gcacacctat tgctgcccac tgtgagggag tgactttatc gagcagctgt
    30241 aaaaaccagt aaccggcact acctgctgag gtgccatagg cgactccagt tgttaactta
    30301 tccatgaatt tcataacccc acctcgcaga caaagcgggt gtaaattgag ggaatacaac
    30361 gtatcgcaaa aaagcagaaa cgtaactgac tccgagtcag tgaataactc aggtattgag
    30421 ttatcagcta atatcgagac tcaaaaaatg gaaaaaccag ctcgacggcg ggtttaagct
    30481 gtgtgacgaa gtaaccactc ttaacagcat aaccaatttt ttacgtacgt aaaccactga
    30541 atgatattta tgagaatgct accgagtgtt caaaacatca ccacaaatac ataagaaaac
    30601 ttcaacaaat aaccaacaaa taatttccag tgttattttt agctggttta aattaaacag
    30661 acgaattata gaaccaccat aaataacagc cattaatata aattagctaa tagatttatt
    30721 tttgttcaaa taagagccat aaataggttt cgatagaaaa agttcagata aaaatagaga
    30781 tctacttcac aaattaaatg agaaactaaa acttacatct tgaaataatc acattgatta
    30841 gatgaatatt tatcgcgcag tgacatcatt ttttaataat agttcaaaaa aaagggctca
    30901 cgatgaaaaa attaacagtg gcaatttctg ctgtagctgc atcagtactg atggcgatgt
    30961 ctgctcaggc agctgaaatt tataataaag acagtaacaa gctggatctg tacgggaaag
    31021 ttaatgccaa gcactacttt tcctctaacg atgcagatga tggtgatact acttatgttc
    31081 gtctgggctt caaaggcgaa acccaaatca acgatcagct gactggtttc ggtcagtggg
    31141 aatatgaatt caaaggcaac cgcgctgaat ctcaaggttc ctccaaagac aaaacccgtc
    31201 ttgcatttgc aggcctgaaa ttcggtgact acggctcaat cgattacggc cgtaactacg
    31261 gtgtagcata cgacatcggt gcgtggactg acgttctgcc agaattcggt ggcgatacct
    31321 ggacccaaac agatgtgttc atgactggtc gcaccactgg tgttgcaacc tatcgtaaca
    31381 acgacttctt tggtctggtt gatggtctga actttgctgc tcagtaccaa ggcaaaaacg
    31441 atcgtagcga tttcgataac tacaccgaag gtaacggtga tggcttcggt ttctctgcta
    31501 cctatgaata cgaaggattc ggtatcggtg caacttatgc gaaatctgat cgtaccgaca
    31561 ctcaagttaa tgcagggaaa gttcttcctg aagtatttgc ttccggtaaa aatgcagaag
    31621 tttgggccgc aggtctgaaa tatgacgcta acaacattta cctggccact acctattctg
    31681 aaacccagaa tatgactgta tttgctgatc acttcgttgc taataaagct caaaacttcg
    31741 aagctgttgc acaatatcag ttcgatttcg gtctgcgtcc gtccgttgct tacctgcaat
    31801 ctaaaggtaa ggatcttgga gtatggggcg atcaggactt agtcaaatat gttgatgtag
    31861 gtgcaaccta ttacttcaac aaaaatatgt ctactttcgt tgattacaaa atcaacctgc
    31921 ttgacaaaaa tgacttcact aaagcactcg gtgtaagcac tgatgacatc gttgctgtag
    31981 gtctggttta ccagttctaa tctgattacg aaaaagatat gttgcgggag gcgttgcctc
    32041 cccaacatat aagtggctcc ctcaagccac ttcctttagg agcacaacct tgcttctaac
    32101 tatataaacc ttctgttata tattaccctt tatttttggg ggcgttgcaa cgccccattt
    32161 ttaataactt tcagtaaaca attggcatat taattagagt tattaacaac gatatccatc
    32221 tctaaccgga tatctaatgc cattaacatc ccttcaatta tgccctcagc cttctgtaac
    32281 cttttcccga tataaccatc agagcagcaa tgcttacctg ccagtgacat gaatgtcata
    32341 ccgactacat aataatctac taataaatcg tgcaaatcgc tgttgttctt tttcagacgg
    32401 gccatgcacc cgcaaatgat catcgcgtca tcgtcacaac attgcgggcg agattttact
    32461 tttgaaggaa ttaatccctt aaaaccggcg gcaatggacg accaggtcac atcttcatga
    32521 ttattagccg cccacgctcc ccaacgttca agaaccatct gaatatcacg cattaacttt
    32581 ctccacaaaa tcaggccagc acaccaatcg ccagtgcgcg atcgataaaa cgaaatatca
    32641 gctccagctg ggagccatac ttctcttcaa atgccacggt atccgcatgc agttcgtcgt
    32701 ggtgttttct gcacaaaggc agcacaaaaa ggtcatgcgc ttttgtaccc attccaccct
    32761 gaccatggcc tatcagatga tgcggatcat cagctggctt tccacaacat gcacacggct
    32821 gcgtcttaac ccagcgcgtg tactgttcat taacccagcg acgacgtttg gggcgtaaca
    32881 taaaagactc cggcgactcc ggatccactt tcagcgccag cacctttttc gctttatcct
    32941 ggataatgct ggtggcagga accgaaggca caaggtcact ttcccgggtg acagacggca
    33001 aaacagactt cggtaatctc agtgccttac gggctgcgct ttccggtaag gcatccgcca
    33061 ggtcattacg aaccagccac cagcacagtt ccggcattgt cacggcatgg ttatcattaa
    33121 aatcgagatc ccgacggact acagacaaca cccaatgggc acagttatcc gttgccattg
    33181 attccagccg ctccgtgaac tgatcacgta actgattatc gcagtgccag cacagacgga
    33241 ttgcgcccgg cgcgtgtcgc attgtggtca tgttctcgct gtgccagtcg gaatgaggcc
    33301 actggcagcc tttttcacga agtaaccagc tttcaagaca ttccacccca ccagcacgac
    33361 gaatcactgc ctcattacgg aacacggccc gaacggcagg atcatccgcc agcggttgtg
    33421 atgccgcggg aacggcacca ctggcgaaag atgaataatg ctccggctca ggctccagca
    33481 gtacacgccc ctgcataaac aggggcatca gctctgaacc gggcctgaac aatacgatcc
    33541 ccatacgcgg ggcaatttca ggggtcagta gtgctctcac ggtcacctca atgaacggta
    33601 tcgagcagct ttaacagctc agggaatcgg gattcgaaga aatgcggctg cgtctcgcgc
    33661 ggatttgcgg gactggtgat gttcttgccg aacatgcagc ctttcgctgt cagcgaccag
    33721 aattttttga tgttgttaat cgcggtacgg ctgtatcgtt cgcgctgctc gacgatcccc
    33781 agcttcacca tctggtgata tgcctgatta gctgtcaggc ggataccata ctgcttcagc
    33841 agtgcactca gtgacagcgt gggacggctt gagccatcag gcgcgtcagc aggagcatca
    33901 atggcatagc gcggtgccag attcggtaag ccaacagcct cctggagttt ctgacaggca
    33961 ccaagcactg aagagttaga caggtttaac tcccggcgca taaagtccag cagaatcacg
    34021 ccagcctgca tcttgtcagc agcctgcccg gataattttt ccgatgcgct ggttaccata
    34081 tcgaaagtac ggatcacctt cagatggaat gacgggctga tccacattgc ataggcatac
    34141 accagttctt tgcagacata cgtcccctgg ttatttccgc cacgaataac gttaactggc
    34201 tctatattga ccgagttgca aatctgcaac tcgcttatta aacgttcagt ttgctcattg
    34261 cggagccaga atgcaggctt atgcttatcc agagaaccgg cagccctgtg cagatcgttc
    34321 aggctgtaac gcccataagc atcacgacga acttcaatac catcaatgac catcagatta
    34381 ttcatacttc gtttctcctc ttgatcaggc ggctgcaccc gccgttttct cgtacttact
    34441 gatagtgatc tcgaccttcc cttccgggat aaccggtccc cactccacca gcattctttt
    34501 cacctggctg tcgtcttccc acacacccgc gtgggtcagg gcgtcaaaca gcgccttgtt
    34561 atagttgtcc agatcgcgga tccggttatc cggaggaaac aacacgatct ccactgaagc
    34621 aggtgccgac gttggtttcg gcagacgacg taactgctca actattgctg tgcacgccgc
    34681 actctggaat tttcgccccg ccgcgcttat caggctctta ccagcaaatg cccctttgtt
    34741 ggggtgtcgc cagtaggtgt tcacgctggg tggaaaaggc agaatcagct tcatactttc
    34801 agccccctct catgtaacca gtgggctgca cgcagcctgg cgttttcctc accagcaagc
    34861 agtgcgcgga taatcccggc cacctcgctg tcgtcgtcct tcaccgcggt atgaagcgtg
    34921 atgccccggg ccacgccacg ctttatcgtg atgacgcctt ttttctccag tgcgcgaaga
    34981 tgctccaccg ctgcattcac tgaacggtat cccagcatgg tagccacctc ctgattggtt
    35041 ggcgggaagc cacgttcttt ctggtaagaa atcagcatat ccagcacctg ctgctggcat
    35101 tgggttaacg tcgtcattaa gcccccacgt aattccctga cagataccac tcttcacccg
    35161 atacagcgcg cttgctgctt ttccgtaaac acagctcacg acgcgccaga aaattgtttc
    35221 gctcttgctg ggagtggctt tcacggaatg ccgccatcca caccgttgca gcacgacggt
    35281 ataagcccct ggactccagt tcttccgcct ggcgggtcag gcacaaaatc acccggggat
    35341 cgttagtgcc gacatagaaa ttgcgcacag gtctggattc acgaactggt tgcggttccg
    35401 gctcctgcgg tatctcagtc agccgcggga aatgtctgcg tgtatcccct tcacaacggt
    35461 gagccacacg cccactctga cgtaacttgc ttgatgactg cagaacgcgc tgtcgtgagt
    35521 aacctgcaaa agcatccgca atgtctccgg aagtacaccc cggatgggct tcaatgaatt
    35581 tctgaacgtc attcaaaaga ctcatgatca ccccctgaat cctgccggga tctggctgta
    35641 gtccacgttg tcgtaactgg ctttgaagta cgggtcttcg cgtttttcgg tgtacgtgct
    35701 gacggacggc gataagcgca gggaaagctc atcccatttt tcccgcagct tcgacgggct
    35761 gagcacgtta cggcaccaga acggatcgcg actgacgcgg ctgtacatct cgcagatttg
    35821 tttatgagta cgaccatcct gcacacacat caggcgaatt tcgtttgccc aggctgtcca
    35881 gttcggttct ttgggacgaa ccacctcgcc gtcacattcg gcggcatgct cgtacagggc
    35941 gatgattttt ttccagagcc actgtgcgca ggtcaaatca tcctgcgtcc cccactggcg
    36001 ctttttaggg ctgaatacaa ccgcatcagg atggcgagtt aaaaaatcct gttcagccgt
    36061 ctgcgtgtcc ggttgcgaag cgtccggacg agaagttttt ttatctgacg gatcatgttt
    36121 tgattttact gacggatccc caccagattc tgacgggtga aaacccgctt ttttgccaga
    36181 tttcgacgca tcaaattttg acgggtcaga ttttgatgcg tcagattttg acgggtcaga
    36241 atctgacagt tgagaaaatg ccgctgcctg aagcttcgca acgttaagct gataaacatt
    36301 cgacgcattg cggttaccct ggcgacgcgc cctacgcgtt aaccagcctt ctgcttccag
    36361 ccgtgcgata gccgttctga cggtactcat ccccgcgcca atctggcggg caatggtttc
    36421 aattgatggc cagcacacac cttcgtcatt actgaaatca gccaggcggg ccataattgc
    36481 cacgctggat aatttcatgc ctgatgcagc gcaaccatcc catacatagc cggttaattt
    36541 agtgctcatg accgacctct atttccctga atttacgacg aaactgttcg agcgggctga
    36601 agcactcatg ctcatagcct tcgcggaggt agataacacg ttgtgtttcc ggctcccaac
    36661 gaatgactct gacgggcact ccgtagtgat ctttgaacca gcggttaact tgtcgcaaag
    36721 gactgtctcc ttctgccggt tgaaatcacc cacagcccac tctgcaaagc tgtgggttac
    36781 aatttccctg tcacctggta catttactgc atagcaatac tccaccttcg cttttccacc
    36841 cggtacagga agcgcaatca gttgcgagcg acggtagtgt gttgttaaac tgttcatgcg
    36901 ttagtttctc cacagtcacg acacgccacg gcgcccggag ctgcacactc gcgggcgtca
    36961 ctactttctg aaacgcaaaa gattttgtag accagtgctg catgctcctg cagcttcgaa
    37021 attgagaggt acagctcatc gttaattgct gtcttctcat gcggttccac tacaccgtct
    37081 tcaattgctg aacgaatctg ttttgaataa ctgccgatct gttcaatgac ttccagcagg
    37141 cgttggttga tatcggcgtt gtccacatcc tcgacatcag gaagagacac aaagacgcca
    37201 tttgcagact gcgccacagc gtcagcaatg aagtgagttc caccagcacg ttgcaaaatc
    37261 attgcccatc ccagcgggaa aatctgatcg ccatcggcac gaaggcggtt aaataatgcg
    37321 ttctctgtta catccagcca gtcagcagct tcagcgtacc cccccggcaa cgctgcgata
    37381 gtttttctga cagctttcac gtaccactca ggctgttttt ctactttcca gtgatgctta
    37441 cccacggttc acctcctgtt cctgtggttt aaacccattc tggttttggc tagattgaaa
    37501 acgtgccgga taaagaatct gcatttcgct gacttcaccc ttaaaaaaat tggctaaacg
    37561 ttctgcaagc tcgatagatg gaatctgctc cagcctctca atacgactca acgtcgctgg
    37621 attgacttga acacccgcag caacatgctg caaagtgaaa ccatgcgcct tacgcacatt
    37681 tcgtaatggt gattgcatat accctccaaa tattgcgcat tatgcatgtt atttcacgca
    37741 attattttgc gcaagttgat ttgcttatca cgcaataaag aaatgtaata aacgcatgaa
    37801 cataggaaac cgagtcagac aacttcgcca agcgaagaac atgaaaatcg ccgatctcgc
    37861 tgaagcaata ggagtagatg cggcgaacat ctcgcgctta gaaacgggta agcaaaaaca
    37921 atttaccgaa caaacactga gtaatattgc caagagctta ggtgttgata ttgctgatct
    37981 ctttacctct gcccacaaaa gtaatactgt atataaaaac agtaataatg aggatgttgc
    38041 gcaggtgaag gatgtgttcc gtattgaaat gctggatatc agtgccagtg cgggaaatgg
    38101 ccttatccag ggcggtgatg tcattgatgt gattcatgcc atcgaataca gaactgataa
    38161 tgctgtatca atgttcggcg gacgaccagc caatcacatc aaagttatca acgttcgtgg
    38221 ggacagtatg tgtccaacca ttgagccagg agatctcatc ttcgttgatg tcagcatcaa
    38281 tcagtttgat ggtgatggta tatatgtctt tggttttgat gacaaaatat acgttaaaag
    38341 acttcaaatg attcctgaca aactgctggt gatttctgat aaccagattt accgtgaatg
    38401 gggaattact agcgaaaacg aacaccgatt catggtcttt ggaaaggtct taatcagtca
    38461 gtcgcaaacc cttaagagac ataattaacc tcaatatccc atccatcggc caccgaaagg
    38521 tggcttttta ctacctataa atttgcatac atcgcaaata tcacttgcat atctcgcaat
    38581 ttaattttat cttttgttcc agaccaacta caggattaca acaaaatctg gttgcaacac
    38641 ggtgcatgtg tcgtaagcag tcagtaaatg tcaaaaacga acaggcagga cgcccacgaa
    38701 gtagccgcct ggggcatatg aagtccagga tgattcgtta gcaacaaaaa agcgccctac
    38761 aggacgctta gctctttagc aatctggata tccacaacag tagtaatcta cagattgccg
    38821 ttaagttttc tggacaactc ctcaatggat ggaggcgata cgtaatccgg atttttattc
    38881 atcagaaact tattttcaca gtggaggcac ctgcttttat gaaaaagctc atcttcgcta
    38941 accgggaatg gttgaagtat cgatactatc ttttgtccaa aacattttgg gcaaagatgc
    39001 atggttatgc tgccaccgtt cacgattacc tccttcgagt atacaaaagt acccgactca
    39061 agttggttaa ggatatagcc ttccgtctga gcctcaaagt tttcgaattc tgcaatttta
    39121 gctttgagag aagcatttat ttcttgataa gagcccacca gttcaacgag agacacgcat
    39181 tcgcgctgaa tagacgcaag ctttgagttc agctcaccaa tagccgcatt tacttcagct
    39241 tgagtttttg cctcgttcat tagttttgca atctgggctg tttcacgaat agccgtcatt
    39301 gctgccgtta attcagcgat cacattgaat actcttattg ttgttgggga tatccagatt
    39361 aaccgaatcc ttgttgttgg ggaataacca ggtccacctc gcctgatgtg gctaaaagca
    39421 ggcacataac agctaagtat tttcaaccag agagaatcct tagcgttgtg gtgaatgcgg
    39481 ctcagcgcac gcgggttaag gttgaggctg acagtcgacc ttctgtggat acccacccgc
    39541 ctggtgtgca accttcgcca ggcaccggga ggcacccggc accacaactt tatgctgtgt
    39601 gtagtcctgg cggtaccagt ttgtaccctt gcttccggct ggtaccgtcc ttttttgcaa
    39661 aacagagaag agcatcaccg gacgacgggc tcataaccca atccatccgg gcggctgcca
    39721 ccgcaggtgt tcttctctgt tttgtggaga aaccaaccga ccttgcaggg tcgatatgat
    39781 gaggagcagc aaaatggcta gcgaacgcag tactgatgtg caggcattta tcggggagct
    39841 ggacggcggc gtatttgaaa ccaaaatcgg cgcagttctc agtgaggtcg cttccggtgt
    39901 gatgaacacg aaaaccaaag ggaaggtctc actcaatctg gaaatcgaac cgtttgatga
    39961 gaaccgtgtg aaaatcaaac acaaactctc atatgttcgc ccaactaacc gcgggaaaat
    40021 ttccgaagaa gacaccaccg aaacgccgat gtatgtcaat cgcggtggtc gcctgactat
    40081 tctgcaggaa gaccagggac aattactgac tcttgccggt gaacctgacg gaaaactccg
    40141 cgcagcaggt cattaatatc gtttttaata aactgattat ttctctcatc actgaatatt
    40201 tttatatagt gaggacttat tatgtctcag aacttagacg caaccgcaat taatcaaatc
    40261 catgccctta tttctgctca gggtgttaat gaaattatca gtaagattgg tgccgatgct
    40321 gtggcattac ctgagaattt ccgcattcat gatctggaaa aatttaattt aaatcgcttc
    40381 cgtttccgtg gtgcgctttc cactgccagc atcgatgact ttacccgtta ttctaaagat
    40441 cttgcagatg aaggcacccg ctgctttatc gatgccgata atatgcgagc cgtcagtgtg
    40501 cttaacctgg gtactattga tgaaccaggt cacgcagata acactgccac tctcaaactg
    40561 aaaaagacag caccgttctc tgctctgttg tctgttaacg gcgagcgtaa ctcccagaag
    40621 tcactggcag aatggatcga agactgggcc gactaccttg tgggctttga tgctaatggt
    40681 gacgccattc aagcaacaaa agcggctgcg gcagtccgta aaatcacgat tgaagcaaac
    40741 cagaccgctg attttgaaga taatgacttc agcggcaaac gctccctgat ggagtctgtc
    40801 gaagcgaaga ccaaagacat tatgccagtg gcatttgaat ttaaatgcgt tccgtttgaa
    40861 ggtctgaaag aacgtccgtt taaattacgc ctcagcatta tcactggcga tcgtcctgta
    40921 ctggttctgc gcattattca gctggaagcg gtgcaggaag aaatggctaa cgaatttcgt
    40981 gatctgcttg ttgagaaatt caaagacagc aaagtagaaa cctttattgg tactttcacc
    41041 gcctgatttc attactgcaa atgcccctgc gggggcattt atggaaacgt aattaactca
    41101 ataatcaccg gatggtgagg gcttcctttt accagaattc agcgcggtgc agtgcatata
    41161 cgtggagaac aaaatgtcat ttattaaaac tttttccggg aagcattttt attatgacaa
    41221 gataaataaa gacgacatcg atattaacga tatcgcggtt tccctttcaa atatctgtcg
    41281 ctttgccggt catctttcgc acttctacag cgtcgcccaa catgcggttc tttgcagcca
    41341 gctggtgccg caggaatttg cttttgaagc gttaatgcat gatgcaacag aagcgtattg
    41401 ccaggacatt cccgcaccac tgaaacgcct tcttcctgac tataaacgga tggaagaaaa
    41461 aatagacgcc gtaatccgtg agaaatacgg gttaccccca gttatgagta cacccgtgaa
    41521 atatgccgat ctcatcatgc tggcaaccga acgccgcgat ctcgggcttg atgatggctc
    41581 tttctggcct gtactggaag gcatcccggc aacagagatg ttcaacgtga ttccactggc
    41641 accgggtcat gcctacggga tgtttatgga acgttttaac gatttatcgg agttacgcaa
    41701 atgcgcatga atgttttcga aatggaaggg tttcttcgcg ggaaatgtgt accgcgagat
    41761 ctgaaagtga atgaaacaaa tgctgagtac ctgttacgta aattcgacgc gcttgaagct
    41821 aaatgtgcgg cactggaaaa caaaataata ccagtgtcag ctgaactgcc accagcaaat
    41881 gaaagtgttc tgttatttga tgctaacgga gaaggctggc tgattggctg gcgttctctc
    41941 tggtacacct ggggacaaaa agaaaccgga gaatggcagt ggacatttca ggtcggggac
    42001 cttgaaaacg tcaatatcac tcactgggca gtaatgccaa aagcaccgga ggctggagca
    42061 taatgaccac atttaccaat aaagaactga ttaaagaaat caaagaacga atcagcagcc
    42121 tagaggttcg agacgatatt gagcgccgtg cttatgaaat cgcactcgta tctctggaag
    42181 tagagccaga tgaacgcgaa gcctatgaat tattcatgga aaagcgtttc ggtgacttag
    42241 tagatcgtcg gagagcaaaa aacggcgata acgaatacat ggcatgggat atgactctcg
    42301 gttggatcgt ctggcagcaa cgagctgtta tccatttttc aacaatgaca cagcaagagg
    42361 tgaaataatg gagccataca gcctcacact cgatgaggcc tgtcagtttc ttcagatatc
    42421 cagaccaacc gccaccaact ggatacgaac aggccgccta caggcaacac gtaaagatcc
    42481 aaccaagcca aaatctcctt acctcacaac acggcaagcc tgcattgcgg cgcttcagtc
    42541 tccgctgcat actgtccagg tgagcgcggg tgatggcata acagaggaaa gaaaatgtca
    42601 ctcttccgca gaaatgaaat atggtatgcc tcgtattcgc tcccgggcgg gaaacgaatt
    42661 aaggaatctc ttggcacaaa ggacaagcgg caagctcagg agttgcacga caagcgaaaa
    42721 gcagaactct ggcgagtaga aaagctaggg gatttacctg atgtcacttt tgaagaggcc
    42781 tgcctaagat ggcttgagga aaaggctgat aaaaaatctc tcgattcaga taaaagccgg
    42841 attgagttct ggcttgaaca ttttgagggt ataaggctta aagatatctc ggaggcaaag
    42901 atttactctg ctgtaagcag aatgcataac agaaagacga aagaaatatg gaaacagaaa
    42961 gttcgggccg ccatcaggaa aggtaaagaa ccgcctgttt atgaaccaaa gccagtatca
    43021 actcagacaa aggcaaagca tcttgccatg ataaaggcca ttctccgtgc tgcagaacgc
    43081 gactggaagt ggctggaaaa agcgcctgtc atcaagatac cagcggtcag aaacaagcga
    43141 gtcagatggc tggaaaagga ggaagcaaaa cgccttattg atgagtgccc cgaaccactg
    43201 aaatctgtcg tcaagtttgc gctggcaact ggtctgagaa agtcgaacat cataaatctg
    43261 gaatggcaac aaatcgacat gcagcgacga gttgcctggg tgaatccaga agagagcaaa
    43321 tcaaaccgcg ccattggtgt ggcgctgaac gataccgcct gtaaagtgtt gcgtgatcaa
    43381 ataggcaagc atcacaaatg ggtgtttgta cataccaagg cggctaagcg agcagatgga
    43441 acatcaacgc ctgcggtcag gaagatgcgc atcgacagca agacatcatg gctatcagct
    43501 tgtcgtcgtg caggaattga agatttccgt ttccatgacc tcagacacac ctgggcaagc
    43561 tggctgattc agtcaggcgt cccattatca gtgcttcagg aaatgggcgg atgggagtcc
    43621 atagaaatgg ttcgtaggta tgctcacctt gcgcctaatc atttgacaga gcatgcgagg
    43681 aaaatagacg acatttttgg tgataatgtc ccaaatatgt cccactctgg aattatggag
    43741 gatataaaga aggcgtaact gattgaattg taatggcgcg ccctgcagga ttcgaacctg
    43801 cggcccacga cttagaagtt cctagaacga cattttaagt caacaaatta ccgcgccatc
    43861 tctgcgctca cacgtcccac gacctcaaaa catgtaaagc cttgcaagcc attgtgaggc
    43921 cttatgtgtc tcagttttgt ccctcttttt tgtactaaaa aacatagcaa ttgaggataa
    43981 acctcatgct attttcgctt atatgccttt aaaggcatgg cgcttaaata gataaaaaca
    44041 ccacaaaagc ataaaaaacc acacagtaaa accacaacat aaaacaataa cagataatta
    44101 aaccaaaaac agatagcgca ttgtgataat cattcaatac taaacaaaat ataaacagtg
    44161 gagcaatatg tgattgactc attaagttag atataaaaaa tacatattca atcattaaaa
    44221 cgattgaatg gagaactttt atgcgggcga aacttctggg aatagttctg acaaccccta
    44281 ttgcgatcag ctcttttgct tctaccgaga ctatatcgtt tactcctgac aacataaatg
    44341 cggacattag tcttggaact ctgagcggaa aaacaaaaga gcgcgtttat ctagctgaag
    44401 aaggaggccg aaaagtcagt caactcgact ggaaattcaa taacgctgca attattaaag
    44461 gtgcaattaa ttgggatttg atgccccaga tatctattgg ggctgcgggc tggactactc
    44521 tcggcagccg aggtggcaat atggtcgatc aggactggat ggattccagt aaccccggaa
    44581 cctggacaga tgaaagtagg caccctgata cacaactcaa ttatgccaac gaatttgatc
    44641 tgaatatcaa aggctggctc ctcaacgaac ccaattaccg cctgggactc atggccggat
    44701 atcaggaaag ccgttatagc tttacagcca gaggtggttc ctatatctac agttctgagg
    44761 agggattcag agatgatatc ggctccttcc cgaatggaga aagagcaatc ggctacaaac
    44821 aacgttttaa aatgccctac attggcttga ctggaagtta tcgttatgaa gattttgagc
    44881 taggtggcac atttaaatac agcggctggg tggaagcatc tgataacgat gagcactatg
    44941 acccaggaaa aagaatcact tatcgcagta aagtcaaaga ccaaaattac tattctgttg
    45001 cagtcaatgc aggttattac gtaacaccta acgcaaaggt ttatgttgaa ggcgcatgga
    45061 atcgggttac gaataaaaaa ggtaatactt cactttatga tcacaatgat aacacttcag
    45121 actacagcaa aaatggtgca ggcatagaaa actataactt catcactact gctggtctta
    45181 agtacacctt ttaacgaagt taaccagatt ttctccccgg tggtattgta taaccccggg
    45241 gaatttgttt acagttacaa gtaggctata tccactaatt ttgtaagcat ttaacctatg
    45301 tcataacgtg taatctctta ccgcaacaca agacctcaat atattcaaat cttatcttta
    45361 ttccgatatt cagagacaca atgcgaaaaa taattactca tttcaaagtt gttttaacgt
    45421 tacttctacc agtaaccgta tctgcccagc agatacagtg gcaatcctgt atggccagtc
    45481 aattcaacca ctggtttggt gaggaaaaac cgtctcctga cttactatgt ggttatttgt
    45541 ctgttccatt aaaatataca gacacaggcg gagatgcttc ttatgaaaaa aaatcacaag
    45601 tcaaactagc gttgacaaaa ttgccggcaa aaagcaagca taaaggaagt atcctgataa
    45661 taagtggtgg tcccgggtta ccaggcataa atccttatat taactttgac tggccagtca
    45721 caaatcttcg tgagtcatgg gatattattg gatttgatcc tcgaggcgtc ggacagtcca
    45781 ctccgacaat aaactgccgg caatcagata cagagactca ggaaaacata accgaaaagc
    45841 aacaagtatt aaataaaatt aatgcctgta tccataatac cggagccgaa gtcattcgcc
    45901 atatcggctc taacgaggct gtatacgata ttgatcgtat taggcaagcc ttgggggata
    45961 aacaactgac agccgtggcg tattcgtatg gaactcaaat tgcagcctta tatgcagaac
    46021 gttttcccta caacgtaaga tctatcgttc ttgatggagt cgtcgatatc gatgacctgg
    46081 aggacaactt cacatggcaa ctcaaacagg cacagagtta tcaggaaacg tttgatcgct
    46141 ttgcatcctg gtgtgcgcgt acaaaaagtt gcccgctttc ttcagacaga gataaggcaa
    46201 taactcagtt ccatgagcta ttatcaaaat tacatcacaa acctttatta gacagtaagg
    46261 gagaaaatat atcttcagat gaactcatat cattaacaac agaccttctg ctatggcgtt
    46321 catcatggcc aacccttgca actgccatac gccagttctc tcaggggatt gtcagtaatg
    46381 aaattgaaac tgcgctcagt gctccgatag cctcagaaga gtcaagcgat gcttcggggg
    46441 taatcctctg tgtagatcag ggggatgagc aattaacacc agaagagcga aaatcccgaa
    46501 aagacgctct tgcgaatgcc ttcccggcta ttaactttga caatggacgt tccgattcac
    46561 ctgatttttg tgaattatgg ccaatacata gcgacctgaa caaaactcgc ctgaaaaata
    46621 ctgttctgcc ctctggttta ctgtttgtag cacacaaata cgacccaaca acgccctgga
    46681 ttaatgcccg taagatggca gagaaatttt ccagcccgtt actaacaata aatggtgatg
    46741 ggcatacatt agctctcacc ggagttaatt tatgtgtaga taaagcagtt gtacatcacc
    46801 tgatcactcc acaaaaaata gaaaatatat actgcccagg aaattctgaa gcagaaatac
    46861 aataatttca aaatattccg ctatttgctc ccaatgcaaa acatatattg cattgggagc
    46921 atattcatat ttttgttatt attgtatgaa atcgtctcat gtacacggtc cttttttcgc
    46981 cgaaaactga tattacttta atcaaaagtt gacgtgccag attcgaagta aatttataaa
    47041 atatcaaatc aatatacatt ttgcaccttc gagatagtaa tgactgttac tgtaacatga
    47101 gttacaaaca gcttcatacc cagagctact gatgtatcaa ctcgattcaa caatacccga
    47161 tatttattcc atgcctccag caacgatctt tcttcctccg ttgcgatttc cagatctaca
    47221 gcatcctgca gtggcgcaat atactcactg aattcctgga tgtagaactg tgtagtgacg
    47281 gtcttccagc cattcggctc ctgctgtatc gaagcatacc aggctatttc aatatcgcta
    47341 tgctgcggca gcatttaacc ccttgtaatt catcgccata attgatttaa ttcacaaata
    47401 aaactataac atggtgaaat taatg
//
LOCUS       DAJTSA010000025        45804 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000025 DAJTSA010000000
VERSION     DAJTSA010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45804
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.025"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            114..2267
                     /locus_tag="OO369_001469"
     CDS             114..2267
                     /locus_tag="OO369_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5981796.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQKK"
     gene            2320..4050
                     /locus_tag="OO369_001470"
     CDS             2320..4050
                     /locus_tag="OO369_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981797.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYNSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINTKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4110..4601)
                     /gene="yajQ"
                     /locus_tag="OO369_001471"
     CDS             complement(4110..4601)
                     /gene="yajQ"
                     /locus_tag="OO369_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR5981798.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4769..5680
                     /gene="panE"
                     /locus_tag="OO369_001472"
     CDS             4769..5680
                     /gene="panE"
                     /locus_tag="OO369_001472"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR5981799.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5643..6233
                     /gene="yajL"
                     /locus_tag="OO369_001473"
     CDS             5643..6233
                     /gene="yajL"
                     /locus_tag="OO369_001473"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR5981800.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6287..7735)
                     /gene="thiI"
                     /locus_tag="OO369_001474"
     CDS             complement(6287..7735)
                     /gene="thiI"
                     /locus_tag="OO369_001474"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR5981801.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7941..8183
                     /gene="xseB"
                     /locus_tag="OO369_001475"
     CDS             7941..8183
                     /gene="xseB"
                     /locus_tag="OO369_001475"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR5981802.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8183..9082
                     /gene="ispA"
                     /locus_tag="OO369_001476"
     CDS             8183..9082
                     /gene="ispA"
                     /locus_tag="OO369_001476"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR5981803.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9107..10969
                     /gene="dxs"
                     /locus_tag="OO369_001477"
     CDS             9107..10969
                     /gene="dxs"
                     /locus_tag="OO369_001477"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR5981804.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNVVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11024..11941
                     /gene="yajO"
                     /locus_tag="OO369_001478"
     CDS             11024..11941
                     /gene="yajO"
                     /locus_tag="OO369_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR5981805.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12052..12570)
                     /gene="pgpA"
                     /locus_tag="OO369_001479"
     CDS             complement(12052..12570)
                     /gene="pgpA"
                     /locus_tag="OO369_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR5981806.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12548..13525)
                     /gene="thiL"
                     /locus_tag="OO369_001480"
     CDS             complement(12548..13525)
                     /gene="thiL"
                     /locus_tag="OO369_001480"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR5981807.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13603..14022)
                     /gene="nusB"
                     /locus_tag="OO369_001481"
     CDS             complement(13603..14022)
                     /gene="nusB"
                     /locus_tag="OO369_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR5981808.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14042..14512)
                     /gene="ribE"
                     /locus_tag="OO369_001482"
     CDS             complement(14042..14512)
                     /gene="ribE"
                     /locus_tag="OO369_001482"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR5981809.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14601..15704)
                     /gene="ribD"
                     /locus_tag="OO369_001483"
     CDS             complement(14601..15704)
                     /gene="ribD"
                     /locus_tag="OO369_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR5981810.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15708..16157)
                     /gene="nrdR"
                     /locus_tag="OO369_001484"
     CDS             complement(15708..16157)
                     /gene="nrdR"
                     /locus_tag="OO369_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR5981811.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16308..16847
                     /locus_tag="OO369_001485"
     CDS             16308..16847
                     /locus_tag="OO369_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5981812.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17145..18029
                     /gene="tsx"
                     /locus_tag="OO369_001486"
     CDS             17145..18029
                     /gene="tsx"
                     /locus_tag="OO369_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR5981813.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18206..18553)
                     /locus_tag="OO369_001487"
     CDS             complement(18206..18553)
                     /locus_tag="OO369_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR5981814.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18682..19653)
                     /gene="secF"
                     /locus_tag="OO369_001488"
     CDS             complement(18682..19653)
                     /gene="secF"
                     /locus_tag="OO369_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR5981815.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19664..21511)
                     /gene="secD"
                     /locus_tag="OO369_001489"
     CDS             complement(19664..21511)
                     /gene="secD"
                     /locus_tag="OO369_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR5981816.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21539..21871)
                     /gene="yajC"
                     /locus_tag="OO369_001490"
     CDS             complement(21539..21871)
                     /gene="yajC"
                     /locus_tag="OO369_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR5981817.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21894..23021)
                     /gene="tgt"
                     /locus_tag="OO369_001491"
     CDS             complement(21894..23021)
                     /gene="tgt"
                     /locus_tag="OO369_001491"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR5981818.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGNIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23076..24146)
                     /gene="queA"
                     /locus_tag="OO369_001492"
     CDS             complement(23076..24146)
                     /gene="queA"
                     /locus_tag="OO369_001492"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR5981819.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24239..24812
                     /gene="acpH"
                     /locus_tag="OO369_001493"
                     /pseudo
     CDS             24239..24812
                     /gene="acpH"
                     /locus_tag="OO369_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24817..26634)
                     /gene="malZ"
                     /locus_tag="OO369_001494"
     CDS             complement(24817..26634)
                     /gene="malZ"
                     /locus_tag="OO369_001494"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR5981820.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26790..28163)
                     /gene="proY"
                     /locus_tag="OO369_001495"
     CDS             complement(26790..28163)
                     /gene="proY"
                     /locus_tag="OO369_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR5981821.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28239..29558)
                     /gene="brnQ"
                     /locus_tag="OO369_001496"
     CDS             complement(28239..29558)
                     /gene="brnQ"
                     /locus_tag="OO369_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR5981822.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29965..31260)
                     /gene="phoR"
                     /locus_tag="OO369_001497"
     CDS             complement(29965..31260)
                     /gene="phoR"
                     /locus_tag="OO369_001497"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR5981823.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLHLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31318..32007)
                     /gene="phoB"
                     /locus_tag="OO369_001498"
     CDS             complement(31318..32007)
                     /gene="phoB"
                     /locus_tag="OO369_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR5981824.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            32197..33399
                     /gene="sbcD"
                     /locus_tag="OO369_001499"
     CDS             32197..33399
                     /gene="sbcD"
                     /locus_tag="OO369_001499"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR5981825.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33396..36539
                     /gene="sbcC"
                     /locus_tag="OO369_001500"
     CDS             33396..36539
                     /gene="sbcC"
                     /locus_tag="OO369_001500"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR5981826.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36665..37846
                     /gene="araJ"
                     /locus_tag="OO369_001501"
     CDS             36665..37846
                     /gene="araJ"
                     /locus_tag="OO369_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR5981827.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37989..38897)
                     /gene="mak"
                     /locus_tag="OO369_001502"
     CDS             complement(37989..38897)
                     /gene="mak"
                     /locus_tag="OO369_001502"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR5981828.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSAWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39022..39933
                     /gene="rdgC"
                     /locus_tag="OO369_001503"
     CDS             39022..39933
                     /gene="rdgC"
                     /locus_tag="OO369_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR5981829.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(40011..40466)
                     /locus_tag="OO369_001504"
     CDS             complement(40011..40466)
                     /locus_tag="OO369_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981830.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40463..40798)
                     /locus_tag="OO369_001505"
                     /pseudo
     CDS             complement(40463..40798)
                     /locus_tag="OO369_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40953..41237)
                     /gene="ppnP"
                     /locus_tag="OO369_001506"
     CDS             complement(40953..41237)
                     /gene="ppnP"
                     /locus_tag="OO369_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR5981831.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41309..41986)
                     /locus_tag="OO369_001507"
     CDS             complement(41309..41986)
                     /locus_tag="OO369_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR5981832.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42244..42435)
                     /gene="yaiA"
                     /locus_tag="OO369_001508"
     CDS             complement(42244..42435)
                     /gene="yaiA"
                     /locus_tag="OO369_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR5981833.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(42485..43009)
                     /gene="aroL"
                     /locus_tag="OO369_001509"
     CDS             complement(42485..43009)
                     /gene="aroL"
                     /locus_tag="OO369_001509"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR5981834.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43192..43650)
                     /locus_tag="OO369_001510"
     CDS             complement(43192..43650)
                     /locus_tag="OO369_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR5981835.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43770..44579
                     /gene="proC"
                     /locus_tag="OO369_001511"
     CDS             43770..44579
                     /gene="proC"
                     /locus_tag="OO369_001511"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR5981836.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44596..45711)
                     /gene="adrA"
                     /locus_tag="OO369_001512"
                     /gene_synonym="dgcC"
     CDS             complement(44596..45711)
                     /gene="adrA"
                     /locus_tag="OO369_001512"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR5981837.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11907 a        11946 c        11206 g        10745 t
ORIGIN      
        1 taagttggca gcattacctt cgcttaattc catataactg gacgattaca ttagaagtaa
       61 actatacttt ttatgaaata tcagggataa attattcaca tggaaaatac atcatgtaca
      121 aatcattatt tactattatc attctactgt atagcgccag ttcatatgca aaaagcgaat
      181 cccctcccgc aagcactata actttcattg cacaaatcga tcatgttgag ctaaataaaa
      241 cagcattatc tcatgatatg aaactcgttc ttgccgatcg tttttatttc aaaaccagta
      301 atccttgcga aacaaattca atttctgctc gaccagaatc aatggatatt actgacaaag
      361 cctatgccga caaattaaaa aaattagaaa ctccttttct tgatgatcgc tatttcttta
      421 tgaccatcaa tcagtgtgac ggtaatggca ctcccatgct gacagacata aaagtatgta
      481 caacagaatt atgtggtgct gaatatacaa agaaaatgtc tactgtatgg ctcgatgaaa
      541 ctttaaaacc aactgctaaa cgtctggcaa tttattttat tcaatctcct ttaccctatg
      601 acgaggagaa aaaacaatgg aaagtcaccg gatggtatat ctcatcaact gataatcagt
      661 cttcaactaa acagattgca tttgaaggat atacagacac agataacctc tccgattcta
      721 aatttatttc ttcatttaaa tcctggtttg aaagtggaaa gataaaaaac attgctaact
      781 ataatccaaa aggagaaaga gatggaaaat acgaaagtta ttacaatagc aatggtaacg
      841 tctatgaatc tctaagtttt tctaatggtc ttatcaacgg tgagtttatt acatattatg
      901 aaaatggggc cattgaaagt aaacgtcatt ttaataacga caaaattaaa gatggtgaat
      961 gccctcattt ttatgaagat gggaaaatca aacagcaaca tagctatctg aatagtaaac
     1021 tcgatggtcc tgcatatgaa tattatcctg atggcaaact catgcaggaa aatttctatc
     1081 agcaaagtgt gttgattgga aacgatacca gttactacca atccggaaaa ataagctcta
     1141 tacacaatag aaattcccgt ggtcagtatg atggagctaa cgaacgttat agtgaagagg
     1201 gtaagttact gtctaaatcc gtctataaag acggaaaaca aatttctgtt caaacctggt
     1261 atgaaaatgg gcagaaagaa gaggagaaac attttgatga gcaaggccaa ttaaatggtc
     1321 tggttaagca atggtataaa aatggtaatc ttgcaaaatc acaaaactat aaacatgaca
     1381 tactcgatgg taacagtgaa gagtggtacg aaaatggcat ccctgaaagt ttgtaccctt
     1441 ataagaatgg aaaaaccgac ggcattgcca aatcatggaa taagtatggt aaattaacct
     1501 attcaatcga atacaaaaat ggcgtagaga atggggcata tcgtaactgg agcaaaaaca
     1561 caggtaaact aaccaaagaa accctgtacg tcaatggtat cagacaggga gtggaaaaag
     1621 agtttaacga tcgcacaggt aaactattaa cctccacgca atatgttaat aataaaagac
     1681 atggtacgga ggaaacttat gatcaaaacg gaataaaata tattacatgc tatcaaaatg
     1741 atcagaaatt atcgtcttta gacaatccaa cacaaataaa agataatgca acaacaggcg
     1801 attccagtgc tcaatttgca ttagggaaat acgaattcat ctgtgcaaat atagatgaag
     1861 ggattaaatg gttaaccaaa tctgcagagc aaaaaaacac tgatgcgata tactttttag
     1921 ctacagccta caaaggcaat ggtatacctg caaacaatga aaaataccta gcatatctgc
     1981 aacaagctgc aacactgggc agtagtaatg cccaggcgga aattggctat ttatatctca
     2041 tcggaaaaga actaccacag aatcttcctg atgctggtgt ctggttcaaa aaagcagctg
     2101 cacaaggtaa ttttgccgca catttctatc tgggaaggat gtatcagaat ggtgacggcg
     2161 ttgagagaaa tatggaaaaa gccaggtttc atctatctaa tgcggcagaa ggtggaatag
     2221 aacctgcgct taaagcatta aatgaacttg aacatcaaaa aaaataaagc gctcagggac
     2281 cacgtaaggt tcatcaaaca gaaaaggata tagtttgcga tgaaagcgaa atccactttg
     2341 ttattaatga ttttatctgt tttttattct catcaggttt tatccagcga acccactcag
     2401 ccggatgttt attctgtcgt cgagaaaaaa ctaaactctg ctttacctct ggaagaaaat
     2461 accaccttca gatcgcaggc tgaatggttt aatttgcaat atgaattatt agtatctggc
     2521 taccctgaac gtgcttacaa tttactgagc aaacttgaga cagaatcgaa aaacgctaaa
     2581 tattatctgg atttaactgc ctccagagtc gctgaagaag aagcaccaga gacagcctta
     2641 acttttttat caaaaattgg gttaaataaa ccggctactc ttacatcctt tgagagctat
     2701 atcaatagct ggataaaaag caaacaaccc gaaaaggcta tgcagcttct cagcctcgat
     2761 gagaatgccc tgaattatta tttaccgact gtgttagtcg catatcagat cgttcctgat
     2821 aaggcaataa gtatttataa ttctgtatat ggagataatg ttgttatacc ctatcagcaa
     2881 gtcaccatgt tacttacagt tgcggagggt tatcaaacaa aaggcgatat aaaaaacgcc
     2941 attcattatg ccgataaggc gctgaatatg tttgataaag ctattgctgt agatagcagt
     3001 cgtgaatccc attatagtga tgagtatttg aaactcatga atatctacgc cgccaatggc
     3061 aacaaagaaa aagcggtggt tttatctcaa cgtctacaca aagccattac cgaatctcaa
     3121 tcgaattact taagtactct ggaaggaatt ttattgtttt atagaaataa tgatatgcag
     3181 caagactatc aaaacagcct ttcaaattac atagtattta tcgataaaat ttttgccttt
     3241 tccccatcaa caagaagtga attaagcctc attaatttat taaattcatt aaatgaggtg
     3301 gaattgatga gaaaacgtct ggcgtttctg atgacagcac cagaatacgc ttgttataat
     3361 agtcagtatt gttatgaaga caaagtcagc gcattaaaaa tcctgtatcg tcatcatgac
     3421 aatgagctaa tggacaaata tttcacgact cttcttaaag agatcagtaa tcaaccatta
     3481 gaagactggg atataataat aagtaatact ggtgaaaaat ttagtgaagt gggtctaacc
     3541 aaatatgctc aacatctcgc atctttcgct gaagaatttt atataaaaga gcaaaaatca
     3601 cattctgaaa gtgaaatgag aggactattt tcatcacttg ccgaattata tagttttggt
     3661 aatgatactg ccagcgcaaa ccgagtgttc catcagcatg tccctgcctt caatacagat
     3721 catatgattc aatattttat aaataccaaa gaatggaaca atgccaggga attattgata
     3781 aaagaggaaa tgttgggtat gcataatctg atattgttgg agaatatttg tatgcaaaaa
     3841 aatgcagaat gcatggcaca cattaccttt acgcttaata aattaacaac acaaccagca
     3901 ataacaaaaa tagatagcgt gggtaatgag caactttatc agattggcaa aatctaccat
     3961 aagttggaaa ttaaacctga gccagagcaa caggttttaa ttcagtcgct ttatgaccgg
     4021 gcatcaggaa gcgcctctgc aactcaataa atgcatggtc aaaagggcca ccacggtggc
     4081 cctgacaatc agtagctata tccagaaagt taatcgcgga agtttttgaa ctggaacggc
     4141 tgaccgagat cgccaccgcg caccatcgcc atgacagcct gcaaatcatc acgagatttg
     4201 ccagtcacgc ggatctcatc gccctgaatt tgcgcctgca ctttcagttt gctgtctttg
     4261 atcatcttga cgattttttt ctgagtggcg ctctcaatgc cctgtttcag tttcgcttcc
     4321 acaaaccagg ttttaccgct atgaacgata ttttccggta catccagtga actgccttca
     4381 atgccgcgct tcagcagctt ggcacgcaga atatccagca actgattgac ctggaagtcg
     4441 gactcgctca acactttgat ggttttgctg gcgtcgttca gctcaaatga ggcttcaacg
     4501 ttacggaagt caaaacggga ctccacttcg cgactcgcgt tatcgaccgc gttacgtgct
     4561 tcctgaagat caacttcaga gacaatatcg aaagatggca tcttttcctc tcccttcatt
     4621 tttgatgcga agcataatac ctgcaaagtt aaataaccaa cagtcctgca ccaggacgta
     4681 tcccttaatt gtgcatagtc gcaacagcat gcacaattaa gggatagcct tattgaacac
     4741 ccggagtggt tgcgggtgag gaggaacaat gaaaattacc gtattgggat gcggtgcctt
     4801 agggcaatta tggcttacag cactttgtaa acagggtcat gaagttcagg gctggctgcg
     4861 tgtaccgcaa ccttattgta gtgtgaatct ggttgagaca gatggttcga tatttaacga
     4921 atcgctgacc gccaacgatc ccgattttct cgccaccagc gatctgctcc tggtgacgct
     4981 gaaagcatgg caggtttccg atgccgtcaa aagcctcgca tccacactgc ctgtaactac
     5041 gccaatactg ttaattcaca acggcatggg caccatcgaa gaattgcaaa acattcagca
     5101 gccattactg atgggcacca ccacccatgc cgcccgccgc gacggcaatg tcattattca
     5161 tgtggcaaac ggaatcacgc atatcggtcc ggcacggcaa caggatggcg attacagtta
     5221 tctggcggat attttgcaaa ccgtgttgcc tgacgttgcc tggcataaca atattcgcgc
     5281 cgagctgtgg cgcaagctgg ccgtcaactg cgtgattaat ccactgactg ccatctggaa
     5341 ttgcccgaac ggtgaattac gtcatcatcc gcaagaaatt atgcaaatat gcgaagaagt
     5401 cgcggcagtg atcgaacgcg aagggcatca tacttcagcg gaagatttgc gtgattacgt
     5461 gatgcaggtg attgatgcca cagcagaaaa tatctcgtcg atgttgcagg atatccgcgc
     5521 gctgcgccac accgaaatcg actatatcaa tggttttctc ttacgccgcg cccgcgcgca
     5581 tgggattgcc gtaccggaaa acacccgcct gtttgaaatg gtaaaaagaa aggagagtga
     5641 atatgagcgc atcggcactg gtttgcctcg cccctggtag tgaagagact gaagccgtca
     5701 ccacgatcaa cctcttggtt cgcggcggta tcaaagtcac cactgccagc gtcgacagcg
     5761 atggcaacct ggcaattacc tgctcgcgcg gcgtgaagct gctggcggat gcgccgctgg
     5821 tcgaagtggc tgatggcgaa tatgacgtga tcgtgctgcc tggtggcatt aaaggcacgg
     5881 agtgttttcg cgatagcact ctgctggttg aaaccgttaa acagttccac cgttccgggc
     5941 gtatcgtcgc ggctatttgc gccgcgccag ccaccgtgct ggtgccgcac gatatctttc
     6001 cgattggcaa tatgaccggc ttcccgacgc tgaaagacaa aattcccgct gaacaatggc
     6061 aggacaagcg cgtcgtctgg gatgcacggg taaaattgct gaccagccag gggccgggta
     6121 cagctatcga ctttggtctg aaaattatcg acctgctggt tgggcgcgag aaagcccatg
     6181 aagtggcatc acaactggtg atggcggcag ggatttataa ttattacgag tagtgtcgga
     6241 tgcggcaaac gtcgcatctg accagatgcg acgtaaaaac tacaaattac gggcgatata
     6301 ccttcacatt gttaaagcct tgctcgcgca gatagagcgc ctgcagacgg ctcatcaccc
     6361 cgcgctcaca ccacagcagc caggttttgt tctggtcgag atcgccaaat ttggtgctca
     6421 gtttatagaa cggcagagaa accacatcaa tcccttcgac tttcagtggc ttatcttcct
     6481 gttcatcgac agaacggata tcgagaatca cgtcgttcgg gccgaagccg ttgacggttt
     6541 ccacttccac cacttcctgc tcggtctgct gggcgatttc gcggatatca acgttattcg
     6601 cttcctcaac cactttatcg agaatactga agtcgaactt ctcttcttcc gcttcaatct
     6661 tcgatttaac cgctttcacc gtcgggcttt tggagatcac gccacaatat tccggcatcg
     6721 tgcgagcaaa gtcttcggtg ccaatctggc gagccaggtt gatgatgtgc tctttgtcgt
     6781 aagaaatcag cggacgcagg atcagcgtgt cagagacgtt atcaatcagg cgcaggttgg
     6841 tcagcgtctg actggacacc tggccgagcg cttcgccggt gaccagcgcc tgtacgccgt
     6901 aacgttcagc cactttagat gcggcacgca ccatcatacg tttgaggata acgcccatct
     6961 gaccgtcgtc gattttctcg agaatttccc cgacaaccgg ttcgaaatta atggcgacaa
     7021 aacgcacgcg gtgggagctg ccaaagcggt tccacagata atgcgccacc tgacgcacgc
     7081 caatttcatg cgctgcgccg ccgaggttaa agaagcagta atgcacgcgg cagccgcgac
     7141 gcatcaacat ataactggaa acaccggagt cgaaaccacc ggaaatgagc gacagcacat
     7201 cttcctgggt gccaatcggg aaaccaccaa taccttcgta gcgaccttta atcagcagga
     7261 ggcgatcgtc ttccacttcc agatgaacag ttacatccgg gttagtcagc ttcacgcgcg
     7321 cggattcaat atgctgattt aaaccgccgc cgacgtaacg ttccacatca atcgagctaa
     7381 aatcatgttt gccacggcgc ttcacgcgta cgcagaaggt tttgccttcc agctgatcgc
     7441 gatactgaac caacgctttc tcaaaaatat cgtgcatgtc ggtaaacggc acgtcttcga
     7501 cttcgagaat atggtggata cccggaatac gggtcagggc gtcgcgaata gtcagacgct
     7561 ggttttcatc tttcgcgcga acttcgatgt tatcccagtg gcggacgaca gcgagcgtct
     7621 catcatagtg ctttaaaacg ttacgaatgt tcccggtaag gatttttata aagcgcaagc
     7681 gcacagattg gcttttgatg gtgatttccg ggaacaattt aatgataaac ttcatggcgg
     7741 caatggttcg ttggcaagcc ttaagcgact tgtataggga aaaatacagc agcccacacc
     7801 tgcggctgca tccaggcgcg gaagtatacc actaacatcg ctttgctgcg cacatcacct
     7861 taccattgcg cgttatttgc tatttgccct gagtccgtta ccatgacggg gcgaaaaata
     7921 ttgagagtca gacattcatt atgccgaaga aaaatgaggc gcccgccagc tttgaaaagg
     7981 cgctgagcga gctggaacag attgtaaccc gtctggaaag tggcgacctg ccgctggaag
     8041 aggcgctgaa cgagttcgaa cgcggcgtgc agctggcacg tcaggggcag gccaaattac
     8101 aacaagccga acagcgcgta caaattctgc tgtctgacaa tgaagacgcc tctctaaccc
     8161 cttttacacc ggacaatgag taatggactt tccgcagcaa ctcgaagcct gcgttaagca
     8221 ggccaaccag gcgctgagcc gttttatcgc cccactgccc tttcagaaca ctcccgtggt
     8281 cgaaaccatg cagtatggcg cattattagg tggtaagcgc ctgcgacctt tcctggttta
     8341 tgccaccggt catatgtttg gcgttagcac aaacacgctg gacgcacccg ctgctgccgt
     8401 agagtgtatc cacgcttact cattaattca tgatgattta ccggcgatgg atgatgacga
     8461 tctgcgtcgc ggtttgccga cctgccatgt gaagtttggc gaagcaaacg cgattctcgc
     8521 tggcgacgct ttacaaacgc tggcgttctc gattctaagc gatgccaata tgccggaagt
     8581 gtcggatcgc gacagaattt cgatgatttc tgaactggcg agcgccagcg gtattgccgg
     8641 aatgtgcggt ggtcaggcat tagatttaga cgcggaaggc aaacacgtac ctctggacgc
     8701 gcttgagcgt attcatcgtc ataaaaccgg cgcattgatt cgcgccgccg ttcgccttgg
     8761 tgcattaagc gccggagata aagggcgtcg tgctctgcca gtactcgaca agtacgcaga
     8821 gagcatcggc cttgccttcc aggttcagga tgacatcctg gatgtggtag gagatactgc
     8881 aacgttggga aaacgccagg gtgccgacca gcaacttggt aaaagtacct accctgcact
     8941 tctgggtctt gagcaagccc ggaagaaagc ccgggatctg atcgacgatg cccgtcaggc
     9001 gctgaaacaa ctggctgaac agtcactcga tacctcggca ctggaagcgc tagcggacta
     9061 catcatccag cgtaataaat aaacaataag tattaatagg cccctgatga gttttgatat
     9121 tgccaaatac ccgaccctgg cactggtcga ctccacccag gagttacgac tgttgccgaa
     9181 agagagttta ccgaaactct gcgacgaact gcgccgctat ttactcgaca gcgtgagccg
     9241 ttccagcggg cacttcgcct ccgggctggg cacggtcgaa ctgaccgtgg cgctgcacta
     9301 tgtctacaac accccatttg accaattgat ttgggatgtg ggacatcagg cttatccgca
     9361 taaaattttg accggacgcc gcgacaaaat cggcaccatc cgtcagaaag gcggtctgca
     9421 cccgttcccg tggcgcggcg aaagcgaata tgacgtatta agcgtcgggc attcatcaac
     9481 ctccatcagt gccggaattg gtattgcggt tgctgccgag aaagaaggca aaaatcgccg
     9541 caccgtctgt gtgattggcg acggcgcgat taccgctggc atggcgtttg aagcgatgaa
     9601 tcacgcgggc gatattcgtc ctgatatgct ggtggtcctc aacgacaatg aaatgtcgat
     9661 ttccgaaaat gttggcgcgc ttaacaacca tctggctcag ctgctttccg gtaagcttta
     9721 ctcttcactg cgcgaaggcg ggaaaaaagt tttctctggc gtgccgccca ttaaagagct
     9781 gctcaaacgt accgaagaac atattaaagg catggtggtg cctggcacgt tgtttgaaga
     9841 gctgggattt aactacatcg gcccggtgga cggtcacgat gtgctggggc ttatcaccac
     9901 gctaaagaac atgcgcgacc tgaaaggccc gcagttcctg catatcatga ccaaaaaagg
     9961 tcgtggttat gaaccggcag aaaaagaccc gatcactttc cacgccgtgc ctaaatttga
    10021 tccctccagc ggttgtttgc cgaaaagtag cggcggtttg ccgagctatt caaaaatctt
    10081 tggcgactgg ttgtgcgaaa cggcagcgaa agacaacaag ctgatggcga ttactccggc
    10141 gatgcgtgaa ggttccggca tggtcgagtt ttcacgtaaa ttcccggatc gttacttcga
    10201 cgtggctatt gccgagcaac acgcagtgac ctttgccgcc ggtctggcga ttggtggata
    10261 caaacccatt gtcgcgatct actccacctt cctgcaacgc gcctatgatc aggtgctgca
    10321 tgacgtggca attcaaaagc taccggtcct gttcgccatt gaccgcgcgg gcattgttgg
    10381 tgctgacggt caaactcacc agggcgcttt tgatctctct tacctgcgct gcataccaga
    10441 aatggtcatt atgaccccga gcgatgaaaa cgaatgtcgc cagatgctct ataccggcta
    10501 tcactataac gatggcccgt ccgcggtgcg ctacccgcgc ggcaacgtgg ttggcgtgga
    10561 actgacgccg ctggaaaaac taccaattgg caaaggcatt gtgaagcgtc gtggcgagaa
    10621 actggtgatc cttaactttg gtacgctgat gccagaaacg gccaaagtcg ccgaatcgct
    10681 gaacgctacg ctggtcgata tgcgttttgt gaaaccactt gatgaagcgt taattctgga
    10741 aatggccgcc agccatgaag cgctggtcac cgtagaagaa aacgccatta tgggcggcgc
    10801 aggtagcgga gtgaacgaag tgctgatggc ccatcgtaaa ccagtacccg tgctgaacat
    10861 tggcctgccg gacttcttta ttccgcaagg aactcaggaa gaaatgcgcg ccgaactcgg
    10921 cctcgatgcc gccggtatgg aagccaaaat caaggcctgg ctggcataat ccctactcca
    10981 ctcctgctat gcttaagaaa ttattcatgg caggagtggc agcatgcaat acaacccctt
    11041 aggaaaaacc gaccttcgcg tttcccgact ttgcctcggc tgtatgacct ttggcgagcc
    11101 agatcgcggt aatcacgcat ggacactgcc ggaagagagc agccgtccca taattaaacg
    11161 cgcgctggaa ggtggcataa atttctttga taccgccaat agctattccg atggtagcag
    11221 cgaagagatc gttggtcgcg cactgcggga tttcgcccgt cgtgaagacg tagtcgttgc
    11281 caccaaagtg ttccatcgcg ttggtgattt accggaagga ttatcccgtg cacaaatttt
    11341 gcgctctatc aacgacagcc tgcgacgtct cggcatggat tatgtcgata tcctgcaaat
    11401 tcatcgctgg gattacaaca cgccgatcga agagacgctg gaagccctga acgacgtggt
    11461 aaaagccggg aaagcgcgtt atatcggcgc gtcatccatg cacgcttcgc agtttgctca
    11521 ggcgctggaa ctacaaaaac agcactgctg ggcgcagttt gtcagtatgc aggatcacta
    11581 taatctgatt tatcgcgaag aagagcgcga gatgctgcct ctgtgttatc aggagggcgt
    11641 ggcggtgatt ccgtggagcc cgctggcgcg ggggcgactg acgcgtccat ggggagaaac
    11701 taccgcacga ctggtgtctg atgaggtggg gaaaaatctc tataaagaaa gcgatgaaaa
    11761 tgacgcgcag atcgcagagc ggttaacggg cgtcagtgaa gaactcggtg caacacgagc
    11821 acaagttgcg ctggcctggt tgttaagtaa accgggcatt gccgcaccga ttatcggaac
    11881 ttcgcgggaa gaacagcttg atgagctgct gaacgcggtg gatatcactt tgaagccata
    11941 acagattgcc gagctggaaa cgccgtataa accgcatgcg gtcgtaggat ttaaataaga
    12001 gtccaggcct gatgagacgt gacaagcgtc acatcaggca tcggtgcaca actacgacag
    12061 aatacccagc ggccagtgat gaccgataaa gtacaggatg cctgcggaaa tcaccccggc
    12121 gacaatatcg tcgatcatga tccccatgcc gccatgtaca ttgcgatcaa accagcggat
    12181 cggccacggc ttccacatat ccagaatacg gaaaatcaca aacccggcgg caacccactg
    12241 ccagtcattg gtcggcagcg ccatgagcgt gatccacata ccaataaatt cgtcccagac
    12301 aatgctgccg tgatcgtgca cgcccatatc tttcgccgtt tgatgacaaa gatagacgcc
    12361 gatacagatc cccaacatca ccaccagcga gtagagctgc cagggtaaaa aggtcatcag
    12421 ataccagaaa gggatcgccg ccagcgatcc catcgagcca ggaacgattg ggcttaatcc
    12481 acttccgaat ccgacagcaa gcagatgcca cggattactc atcttcaggc gacttttcgc
    12541 gacatcttta tggcgtgaca aaatggtcat atcctttcca gtcaaacgtg acaggttcgc
    12601 cgtcacgaat aaaacaaagc ccttcgatat cggcggtcat ttgcccgata caggtaaacg
    12661 gtacgccaag gtgtccgaga gcaacatcca gcgcgccacg gttcagttcc ggcaccgtga
    12721 aacacaactc gtaatcttca ccgccagaga gcgcccagcg cagcgcctgt tccggttcaa
    12781 catggcgaga aagcgcatca gaaaacggca gcaatgccag gtcaatacgt gcgccgcagt
    12841 cgctggcttt cacgatatgc ccgagatcgg aaatcagacc gtcagagaga tcgatagctg
    12901 aatttgccag atcgcgcagt gcctgcccct gtaaaatacg cggcgatgga cggagatgac
    12961 gtttgatcaa gtagtcagca tctttagcat cggcaacctg caaacggttt tgcaaaatcg
    13021 ccagcccggc ggcgctatcg cccggtgtac cggtcacata gatccagtca cccggtttcg
    13081 ccccagagcg cgttaaggct cgtcccatcg gaacaaagcc gtggataccc aacgtcattg
    13141 ataatggccc acgcgtggta tcgccgccaa tgagttgcat atcgtaataa ttgagaagat
    13201 caaacaaact gtcgctgaag gactcaagcc acgcttcgtc tacgtccggt aaggttaatg
    13261 ccagcgtcag ccaggccgga tcggccccca tcgctgccag atcgcttagg ttcaccgcca
    13321 gtgctttata agccagatca gcaggatcga tatcagggag gaaatggttg cccgccacca
    13381 gcgtatcagt gctgatcgcc agggtctgtt tctcggggat attgagaaga gcgcaatcgt
    13441 cgccgatgcc cagttcgaca tcaagacgag aacttcttac acggtcaaaa taacgggcaa
    13501 tcagggagaa ctcgccacat gccatacgtt atgcctcagc aggtaaaaaa gaaaaggccg
    13561 gcgatcatgg aacagtcttc cgtgaatcta ccggcctgga tattactttt tgttagggcg
    13621 aatcacaggt gctgctttat cgagtacgcc gttgacgaac ttatggctgt cttctgcgcc
    13681 gaacgatttc gccagttcga tcgcttcgtt aatggccact ttgtatggca catcactacg
    13741 tttagacagt tcgtacagcg caatgcgcag tactgctttt tctacctggc ccagttcttc
    13801 cagcaggcgg gacaggtagg gcttcatcag tccgtcgagg tatgcggtgt tagtcgccac
    13861 cccggccagc agctcacgga agtacaggac gtcaacgtct tttacatcct gttcagccag
    13921 gaactggtat tcaacatcag cgatgtcgtt ctgggacaac tgccaggagt agagcgcctg
    13981 gacggcacac tcacgagcgc ggcgacgagc agcaggtttc acggatttcc ccttactaat
    14041 ttcaggcctt gatggctttc aatacattaa tcatttcaag cgcggtcagt gcagcttctg
    14101 cacctttgtt gccagctttg gtgccagcac gttcgatcgc ttgttcaatg ctttcagtgg
    14161 tcagaacccc aaaagcaacc ggaatttcgc tgtcctgggc aacatgcgcc aggccgttgc
    14221 ttgcaccacc agcgacatat tcaaagtggg cagtgccacc acgaataacc gtacccagcg
    14281 caatcaccgc gtcgtattta ccggttttag ccagtgcacc cgccgccagc ggcagctcat
    14341 aggcaccagg cacccaaaca acggtaatgt tttcatcttt tacctgaccg atacgtttca
    14401 gtgcgtcaat tgcaccttcc agcaggctgt cattgataaa gttgttgaaa cgcgcgatgg
    14461 tgatggcgac gcgagcgtcc ggggtagcaa cgttagcttc aataatgttc attttcttcc
    14521 ttcgggttcg agtatggccc cgcagggggg cggattttag cataatattt cgtgcgctgc
    14581 ttccctttcg agccgggaga tcatgcaccc actaaatgca ggcaaacatc cgggcctaca
    14641 tgacgtatct ctttgaattt aaattggggg gcgtcggcta atttctcaag ccctggcagc
    14701 gagcataatc cacgggcgtc gctgcctaat agtttaggtg cgatatagac aatcagctca
    14761 tcgactaaac ccgcctgtag caatacgcca gcgagcgttg gccccgcttc cacccagatg
    14821 ctgttaatct gctgtttacc cagttgcatc atcagtacaa ccagatccag atgaccttta
    14881 tgctctggaa tcagcaaggt acgcaccgtt tccggccact cacgagaatc ttcctgcgta
    14941 cgcgcgaacc aggtttcgcc gggctgctgc acaatgcgat gttccggcgt cacgcgattt
    15001 tggctatcaa tcacaatacg tactggctgg cggagatttt gttgcgggta gagcgcctga
    15061 gtttgttcat ccagttcaga ccaacgcact gttaaggctg gatcatccgc cagcaccgtg
    15121 gcgctgctgg ttaaaatggc atgactttgt gcgcgctgtc gttgtacgtc gcgccgcgcc
    15181 tgaggcgaag tgatccactg gctttcgccg ctcgccatcg ccgtgcggcc atcaagcgat
    15241 gcgccaagtt ttaactgaat ataaggaaag ccggtgcgca tccgcttgag aaagccttta
    15301 ttcaattgct cggcttcact catcatcagc ccgtggctga cgtcaatgcc agcctgttgc
    15361 agacggtaaa gtccacgccc agcgacctgc gggttaggat cttgcatcgc tgcgaccacg
    15421 cgcgctacgc cagcggcgat gagtgcgtca cagcacggtg gcgtacggcc atgatggcta
    15481 cagggttcga gtgtcacata cgccgttgcg cctttggctt tttcacccgc catacgcaac
    15541 gcgtgtactt cggcatgtgg ttcacccgct cgttggtggt aaccttcacc gacaatttcg
    15601 ccatctttga caatgacgca cccgacattc gggttggggt gggtggtaaa acgtcctcgt
    15661 tgcgccagct ttagcgcccg cgccatgtaa tactcgtcct gcacggctta gtcctccagg
    15721 cgcgcgatct cttcgccaaa ttctttgata tcttcgaaac tgcggtagac agaggcaaaa
    15781 cggatatagg cgactttatc gagctttttc aattgctcca tcaccagatt gccaatcatc
    15841 ttgctcggta cttcgcgctc accggtggcg cgcagctgcg atttaatatg attgattgcc
    15901 atttcaacgt aatcggaact caccggacgt ttttccagcg cccgcagcat tccgctacgc
    15961 aatttctctt cattaaacgg ttcacgcacg tcgttgcttt ttacaacacg cggcataacc
    16021 agctccgcca cttcaaaggt ggtgaaacgt tcattacaca ccagacactg ccgacggcgg
    16081 cgtacggatg aaccctcgcc cacgagacga gagtcaatta ccttagtgtc cacggcgaaa
    16141 cagaatgggc aatgcatacg gcgtcctgac caggtggtta acggaaatct attttaccct
    16201 gaactgacga tacaacaaag gcgcaacgct tttgagataa aggatcgatg ctttcgctgc
    16261 ttttaccctc taccattagg ctaattgcga cgagaaaggg acgacctatg aatactaacg
    16321 tttttcgact gctcctgctg ggaagcctgt tcagccttag cgcctgtgtg cagcaaagtg
    16381 aagtgcgaca gatgaaacac agcgtcagca cgctgaacca ggagatgacg caactcaatc
    16441 aagaaacagt caaaatcact cagcaaaaca ggctgaatgc aaaatccagc agtggggttt
    16501 accttctgcc tggagcgaaa acaccggcaa gactggaaag ccagatcggt actttacgta
    16561 tgtcgctggt gaatattacg cctgatacag atggcaccac ccttacacta cgtattcagg
    16621 gtgagtcgaa tgatcccttg cccgcattca gcggaactgt tgaatatggg caaattcagg
    16681 gaacaataga caactttcag gaaatcaatg tgcagaatca attgattaat gcccctgcca
    16741 gcgtcctcgc ccccagcgat gttgatattc cgttacaatt aaaggggatt tctgtggatc
    16801 agttaggctt tgtacgtatc cacgacattc aacctgttat gcagtaaacg tatttcggga
    16861 cgattttgtg cgtcccgcaa catcttcccc gtcattttgt tactctgctt acatcacctg
    16921 gattgatagt aaaagtttgc aacaagggcg aaagtcagta caatccccgc ccgaatgtgt
    16981 gtaaacgtga acgcaatcga ttacgtaaat gatagaactg tgaaacgaaa catatttttg
    17041 tgagcaatga tttttataat aggctcctct gtatacgaaa tatttagaaa cgcaatttgc
    17101 gcctttttca ctcccgcaag ggattttcaa acagtggcat acatatgaaa aaaacattac
    17161 tggcagccgg tgcggtactg gcgctctctt cgtcttttac tgtcaacgca gctgaaaacg
    17221 acaaaccgca gtatctttcc gactggtggc accagagcgt taacgttgtc ggaagctatc
    17281 acacccgttt cggaccgcag atccgcaacg atacctacct tgagtacgaa gcattcgcta
    17341 aaaaagactg gttcgacttc tatggttatg cggatgcgcc ggtattcttc ggcggtaact
    17401 ccgatgctaa aggtatctgg aaccacggtt ctccgctgtt tatggaaatc gaaccacgtt
    17461 tctccatcga caagctgacc aatactgacc ttagcttcgg tccgttcaaa gagtggtact
    17521 tcgcgaacaa ctacatttac gacatgggtc gtaataaaga tggtcgccag agcacctggt
    17581 acatgggtct gggtaccgat atcgacactg gcctgccgat gagcctgtcc atgaacgtct
    17641 atgcgaaata ccagtggcag aactatggcg cagcgaacga aaacgagtgg gacggttacc
    17701 gtttcaaaat taaatacttt gtgccgatta ccgatctgtg gggcggtcag ctgagctaca
    17761 tcggcttcac caacttcgac tggggttccg atttagggga tgacagcggt aacgcaatca
    17821 acggtattaa gacccgtact aataactcta tcgcttccag ccatattctg gctctgaact
    17881 acgatcactg gcactactct gtcgtagctc gttactggca cgacggtggt cagtggaacg
    17941 acgatgcaga actgaacttc ggcaacggca acttcaacgt gcgctctacc ggctggggtg
    18001 gttacctggt agtaggttac aacttctgat tatgaaaatg ccgggattta ttcccggcat
    18061 ttctgattgt taacaacgtg tgctttgttc atgccggatg cggcgtaaac gctttgcccg
    18121 gcccacaaaa gcacgtaaat tcaatatatt gctgaaataa cgtaggcctg ataagcgtag
    18181 cgcatcaggc aattttacgt tttaatcact tcttcttgtt catcatcgct ttcaggtcag
    18241 cgaatgggtt gtacttcgct tcaccgacat ctttctgcgc atcttcccct gcgataacgg
    18301 tcgtaccata ctgatccgct tcggtatatt tcgaatgctc atgatcgtgg caatagagac
    18361 acaacaattc ccagttactg ccatcttccg ggttattggt atggtcgtga tcaatgtggt
    18421 gaacggtaag ttcacgcagg ttagaataaa caaactcgcg ggaacagcga ccgcagaccc
    18481 acggatagat ttttaatgct ttttcgcgat agccgctttc taaccgtgca tagttttttg
    18541 ggatgatagc catgaaaagt tacctggatg aaatgtaggg ttacgcgcag tttcccaaga
    18601 tgcggggatt ttcgcaatct ccatacatca aaaaaaatcc cgatcttctg accgggattt
    18661 tcaacatcaa cgggaacttg attaaggcag aattgacggc tgatccgccc cttctttttc
    18721 cactttctgc tgcaacatgt gttcgcgctt catacccagt ttcagagcca acgcagatgc
    18781 tacgtagatg gaagatgcag tgccgatgga aacaccgata agcatggtca gcgagaagcc
    18841 ttccagtacc ggaccaccga agagatacag catcaggata accatcaagg tagtaccgga
    18901 tgtgatcaag gtacggtgca gcgtctgggt caaggacacg ttaaagattt cgtaaggcgt
    18961 accgcgacgg atcttgcgga agttttcacg aattcggtcc gataccacga tactgtcgtt
    19021 aagcgagtaa ccgataaccg acatcaacga tgccacaatg gtcaggtcaa tctcgatatg
    19081 gaataacgac aaaataccca gcgtaatgat aacgtcgtgc gtcagcgcaa taaccacccc
    19141 tgccgccagt cgccactcaa agcggaaacc tacgtacacg aggatagaca gcagcgctgc
    19201 catcaacgcc atcgcaccgg tttgcgcaag gtcagccccc acgctcggac cgacgaactc
    19261 aatacgcttc actgctgcat tctgattggt ggattcgtta atcaccttca gaacttggct
    19321 gcccagcacc tgaccgccgg tttcgccttc agcaggcggc atacggacca tgatgtcgtg
    19381 gctgctacca aagttttgca gcattggctc ttcaaaaccg gctttttgca atgcatcacg
    19441 cattacgtca atttcagccg gtttttcgag cgtaatttcg ataaccgtac caccggtgaa
    19501 atccagcccc cagttaaagc cgcgcacgcc cataataacg atagcagcga ttaacagcag
    19561 accagagatg ccgaaagccc agtagtccca gcgcataaag tcatagactt tacggccgtg
    19621 gtttagttgt tcaacagtat attcctgtgc cacatcgcac tcctcagatt gacagcttct
    19681 tgacgcgctt gccgccatat agcaggttta cgatggcacg cgtaccgaca atcgcggtaa
    19741 acatcgacgt cgccacaccg ataccggtag taatcgcgaa ccctttaatt gccccggtac
    19801 ccactgcgta caggatgatg actttaatca gcgtggtgat gttcgcatcg aagatagaac
    19861 tgaatgcgcc acgataacct tcatcaattg cctgttgaac agtacgtccg ttgctcaact
    19921 cttctttaat acgttcgttg atcagtacgt tcgcatcgac cgctaccgca agggttaaga
    19981 cgatacccgc gatacctggc atactcagtg ttgcgcctgg caacagcgac ataataccga
    20041 cgatcaagat caagttggca atcagagcac tggtcgcaat cagaccaaac ttcttataga
    20101 agatgatcat gaacagaata gaaaccagca gaccggcaag gcaagcctcc agcccctgct
    20161 caatgttctg catacccagg gtcgggccaa ttgtacgttc ttcaacaatc tgaatcggcg
    20221 cgatcaacgc accggcacgc agcagcagtg acagctgacg ggcttcgttc gggttgttga
    20281 tgccggtgat acggaagctg ttacccagac gagactggat gttggcgatg ttaatcacct
    20341 cttcctgttt caccagaacc gcacgaccat ttgcatcttt cttaccgctg tctttgtact
    20401 ccacaaacag ggttgccatc ggtttgccga tgttgtcctt ggtgaagtta gacataatgt
    20461 tgccaccagc gctatcgagc gagatgttaa cctgcggctg gttgtattcg tcctggctgg
    20521 aagtggagtc ggtgatatgg tcaccagtca gaattacgcg tttgtacagc acaaccggct
    20581 gaccttcgcg ggtctgtttc acttcagagt cgcccggtac gcgaccggat gccgcagcgg
    20641 cctgatcaac attggtgttt accagacgga attccagcgt tgccgtcgcg cccagaatct
    20701 ctttcgcacg cgcagtgtcc tgaataccgg gcagttcaac aacgatacgg tcagcaccct
    20761 gacgctgaac caccggctcc gccacgccaa gttggtttac acggttacgc aggatattaa
    20821 tgttctgctg caccgcatat tcacgcgctt cactcagacg agcatcgctc attaccgcac
    20881 gcagctggtt gctgccctgg ctgctaatca ccaggtccgg atggcgcttg ctcagatacg
    20941 caatggcttc atcacgagct ttagcatcgc ggaaagtgat gctcaggccg tagttgtttt
    21001 ctttacgaac agtggtatac gggatgccct tttcgcgcag gtcactgcgt aggctatcga
    21061 tattttgttc ctggagtttg ccaagcgcgg tgtccatatc cacttccatc aggaagtgaa
    21121 cgccgccacg caggtcaagg ccgagcttca tcggctcagc atgaatagct gccagccagc
    21181 gcggcgttgc cggggcaagg ttaagcgcca cgacgtattt gtcacccata acgcccatta
    21241 atgcttcacg agcgcgcaac tgggtgtcag tggagtcgaa gcgcgcaaga atagcgccct
    21301 cttccagtgc cacagactta gcagttattt tttcttcttg taacgttttc tggacctgga
    21361 tcagcgtttg ctcactggcg gcgactccgc gcgcaccagt gatctgaaca gccggatcct
    21421 caccaaacag gttgggaagc gcatacagca gaccgatgac aatcaccacg atcagcatga
    21481 cgtacttcca caaaggataa cggtttaaca cggcaattcc cttagggaaa aattttaatt
    21541 acagcgcctt catggtgcct ttcggcagga cggcagctac gaagtcacgt ttaataacta
    21601 cttcagtggt gtcattcagc gcgatagcaa tgtagccgtt ttccgctact ttggttacgc
    21661 gaccaaccag gccaccgttc gtcagaactt catcaccttt ggcaatggag tccatcagct
    21721 ttttgtgttc tttggtgcgc ttctgctgtg gacgcaggat catgaaatag aaaatcagac
    21781 cgaataccac cagcatcaaa atcaaagaca tcgggctacc ttgcgccggt gcacccgttg
    21841 ccgctaccgc atcagaaata aaaaagctca ttaaatttcc ctcattatta atattaatca
    21901 acgttcaaag gtggtacttc tcgcccctga cgctggtaaa aatcagttac gaagctctct
    21961 aatttaccct cttcaatagc cttgcgtaaa cccgccatca aacgctggta gtaacgaagg
    22021 ttatgaatgg tgttgagtcg cgcgcctaat atttcgttgc aacggtcgag atgatgcaag
    22081 taagcgcgtg aataattgcg acaggtgtag caatcacact caggatcgag tgggccagta
    22141 tcgctcttat atttcgcatt gcggattttc accacgccat cggtcacgaa caaatgacca
    22201 tttcgggcgt tgcgggttgg cattacgcag tcaaacatat cgataccgcg acgtacgcct
    22261 tcaaccaggt cttccggttt accaacgccc atcaggtaac gcggtttgtc tgccggaatt
    22321 tgcggacaca catgctccag aatacggtgc atatctgctt tcggctcacc cacagccaga
    22381 ccgccgacag cgtagccatc aaagccgata tctaccagac ctttaacaga tatatcacgt
    22441 aaatcttcgt aaatactgcc ctgaattata ccaaataacg catttttgtt accaagactg
    22501 tcaaaacgct cacggctacg cttcgcccaa cgcagagaca tctccataga gcgttttgcg
    22561 taatcccagt cagcaggata cggcgtacac tcatcaaaga tcatgacgat atccgaacca
    22621 agatcgtact gaatctccat cgacttttcc gggtcgagga aaatcgggtc gccgttgatc
    22681 gggttacgga agtgaacgcc ctgttcggtg attttacgaa tattaccaag gctgaagacc
    22741 tggaagccgc cggagtcggt aagaatcggt cctttccact gcataaaatc gtgcagatcg
    22801 ccgtgcagtt tcatgatttc ctggcccggg cgcagccaca ggtggaaggt gttgccgagg
    22861 ataatttgcg cgccagtggc ttcaacttct tccggcgtca tcccttttac ggtgccgtag
    22921 gtgccaacag gcataaaaca aggcgtttcc actacgccac gatcaaagac caggcggcca
    22981 cggcgtgcgc gaccgtcggt ggtgtccagt tcaaatttca ttttttctcc actacgtcag
    23041 aaaaacagtc caacgtttaa accagcgccg cggaattact ccccgacgcg ctcattaatt
    23101 gcctgcggat tgtacgtgat aaacatcgca tcaccgtaac taaaaaagcg atatttctct
    23161 tctaccgccg ctttataggc attcatggtg tgttgataac cggcaaaggc cgaaaccagc
    23221 ataatcagcg tcgactctgg caagtggaag ttcgtcacca gcgcatcgac cactttgtac
    23281 tggaagcccg gatagataaa gatttgggta tcgtcaaaga acggttcgat aagatcgttt
    23341 tttgccgcct gtgccgcgct ttccagcgaa cgtactgaag tggtgccaac cgcaatcacc
    23401 cggttaccgc gcgctttcgc cgccagtacc gcgtctacca catcctgcgg tacttcagcg
    23461 tattccgagt gcatgatgtg atcttcaatg gtgtcgacgc gcaccggctg gaaggtgccc
    23521 gcaccaacgt gcaacgtcac aaacgccatc tccacgcctt tggcgcgtaa tttttccagc
    23581 aaaggctcgt caaaatgcag accagcggtc ggggctgcaa ccgcgcccgg tttttcgcta
    23641 taaacggttt gataaagttc gcggtcagcg tcttcgtccg gacgatcgat atacggcggc
    23701 agcggcatat ggccgatgct gttgaggata tccagtaccg agcgttcatc attaaattcg
    23761 acttcaaaca gtgcgccgtg gcgcgcggtc attgttgcgt tgatactttc gtcatcgccc
    23821 agcaacagtt ctgcgccagg ttttggcgct ttcgaggcgc gaatatgcgc aagaatgcgt
    23881 ttgtcgtcga gcatccgctc aaccagcact tcaatcttgc cgccgctggc tttacgccca
    23941 aacagacgcg ccgggatcac gcgggtatta ttaaaaacca gaagatcgcc ggggttgagc
    24001 ttatcgagta aatcggtgaa agtaccgtgc gtcagcgcgc ccgtcggccc gtccagcgac
    24061 agtaaacgac agctactgcg ttcaggcatg ggatagtggg caatcaggga ttcgggcaat
    24121 tcaaaggaga aatcggtaac gcgcatgaca ctgactcaga ctaaaataag agtcgggtag
    24181 tctagtgccg gggcgctctc cctgcaacct ttacccccct ttggatagaa tataatcgat
    24241 gaatttttta gctcacctgc atttagccca tctcgcggaa agctcgcttt ctggcaattt
    24301 actggctgat ttcgtacgcg gaaatcctga agaaagtttt ccgcccgacg tcgtggctgg
    24361 cattcatatg catcgacgta ttgactgaca atctgccgga agtccgcgaa gcacaggagt
    24421 ggtttcgtag tgaaacgcgc cgcgttgcgc ctattacgct ggatgtcatg tgggatcact
    24481 ttctttcccg ccactggtcg cagctgtcgc cggactttcc gctacaggaa tttgtctgtt
    24541 atgcccgcga gcaagtgatg acgattttgc cggactcacc gccacgtttt atcaatctga
    24601 acaattactt gtggtcagag cagtggctgg tgcgctatcg cgatatggat ttcatccaga
    24661 acgtgttaaa cggcatggca agccgccgcc cacgtctgga tgccctgcgt gactcctggt
    24721 acgatttaga cgctcattat gacgccctcg aaacccgctt ctggcagttt tatccgcgga
    24781 tgatggcgca ggcgtcacac aaggcgttat aaaacgttag ttcatccata ccgtagccga
    24841 aatggcaggc aaagccagaa tcccgtcagt cagttgccca tgcccttctt tgcattgcca
    24901 ttgcacggca ttgagaaacg gtgacgcggg tagcaccact tcactggcct cgccacggtt
    24961 gattgccacc agtacacgtt gctgattcag cacgcggaca aataccacta cgttatcttc
    25021 cgcatacagc acctgacagc cgccacgacg cagcgcctga cttttcttac gcagcacaat
    25081 cattcgccgg tacagcgcga ataacgccgt atcctgcttt tccacctgcc aggggaacgg
    25141 tttacggcaa aacggatcgt ttttaccatc cagtcccact tcatcaccgt aataaatgca
    25201 cggtacacca ggccaggtga acagccagac caccgccagc ggcaggcgcg caatatcccg
    25261 accgagcagc gttttaaatc gcgcagtatc gtggctatcg agctgattaa acatacgtaa
    25321 ttgttgttga tgagaaagcc ctgcgcggta attatccatc caggccatac aggtttgggc
    25381 gtcaatttgc tgcggatcat aagagatgtc ggtattggca agaaatcccc acaacgggaa
    25441 tgtgaagcca cgatagttca tggcagcatc ttccacatcg gcctgtaacc attgccgtgc
    25501 atcgccaaaa tgttcgccga caatatacgc ttccggctgg gtttctttcg ccgcttcggt
    25561 gatcccggca acgtgctgca tattattgcg cgccccaccc gcctccccca gcatatgcac
    25621 cacatccagc cgccagccgt ccatattcca cggcgctttc agccagtggc ggacaatact
    25681 gtcttccccg cgataaattt cattcaccag actttccgac tgataatcca gcttcgtcaa
    25741 gctggcatag ccaagccagt cgagcgccgt gccatcatca ctaaacgagt accagtcgcg
    25801 ccagggcgat tcagagttgt gacaagctcc ccccgtgcca agattgtgcc tgtcaaacca
    25861 ggcatgggaa tcgccactgt ggttaaacac gccgtccagc accagccgca ttcccagctg
    25921 ctgcgtattg tgtcgcaaac gcagcaacgc cccatcaccg ccaaactgcg gatcgacatg
    25981 gcgataatcc tcggtatcgt atttatgtac gctgggagct ttaaacaccg gattgagata
    26041 cagcgctgtc acgccaagct ttttcagata cggcagtttt tcgcttatcc cgtccagatc
    26101 gccgccatag aacgttgatc cgcccgcctg cgccgtgacc ggttcatccc agtcacgcaa
    26161 gatgatctct tgtccggctg catgatggta atagacatga tcctgttcag cttcacgagg
    26221 aagactacgc gcaaaacgat cagggaagat ctgataaaaa atctgatccg cagcccattg
    26281 tgggccgata tcaggtacat cgacggcaaa ctgctccagt cgtgccggcg gcattcggct
    26341 gaagccctgc ggtgtaaacc aacgctggcg atcgtgccac agcagtttga aactgtaacg
    26401 ccgccggggt tgtccgctgg agagatcaat cgccgcacgc catgcggtga cgcctggctg
    26461 cggctgactg cgctgcttat gcattggtac tgacatttct tcgttatcgt gttctgtacg
    26521 cagcataatg cgctgcggtg ggtcttcgcc cgtcagccac agtgtaatga gcagttgatc
    26581 tttgctttgt ttaacaaatg ggggcaccgg caggtgccat gcatttaaca tcataattcc
    26641 cctgtgatga aaatctgatc accttgccat agcctaatgc aggatcactc atcatctgaa
    26701 ggatatttgg gggaggagga cgggggcgga taactttcgg aatagccgga tcaggatttc
    26761 ctcctgaacc ggcttacaga acgcaagtat tattggtttt cagccagctg acgatcgtgg
    26821 cggcgtttaa acatccagcc aatcaacagc acaacaatcc acgcgaaacc gacatacagc
    26881 gagatacgcg tatccgggtg ataaccaatc aacccgataa taaagagcag gaaaatcagc
    26941 ccgccgatgg tcgttgctac cccacccggc actttaaatt tcagcgcctt aacttcttct
    27001 ggcggcaaac ggcgacggaa ggcgatttgc gacagcagga tcataatcca cacccatacc
    27061 gtggcgaagg ttgccagcga agcgatcacc aggaagacgt tttccggcat gatgtagttc
    27121 agatacaccg caaacagcag cgcggtagtc atcaccagca ccgtaaccca cggaataccg
    27181 cgacgcgacg ttttgctgaa aattttcggt gcgctgccct gctctgccat accgtggagc
    27241 atacggccta cgccaaatac gtcactgtta attgccgaca gcgaagcggt cagcacaaca
    27301 aagttaagaa tgctggcggc aaaggtaatg cccatatgct ggaacgtcag tacgaacggg
    27361 ctaccggcag tgccaacctg attccacggg tagatagaca taatgacgaa cagcgtaccg
    27421 acgtagaaca ccagaatacg catcggcacg gagttaatcg cacgcggaat cgatttctca
    27481 ggatctttcg cttcaccggc ggtaatcccg ataatttcga tcccaccgta agcaaacatc
    27541 accatttgca acgacattac catgccaagc cagccgttac tgaagaagcc gccgttgctc
    27601 cacaggttat gaataccggt cggttgcccg ccgttgccaa tcccccagat gatgatgccg
    27661 aaaccggcga caatcatgat gatgatggtg gcgactttaa agaacgagaa ccagaattcc
    27721 agctcaccga agaccttcac gctcatcagg tttacggcgc aaatgatcag caccacgctc
    27781 agtacccaaa tccagtgcgg caccgtcggg aacaagacac ccatatagat accaaaagcg
    27841 gtcacatcgg cgatggcgac aataaggatt tcaaaacagt aggtccatcc ggtaatgtaa
    27901 cctgccagcg ggccaaggtt ttcctgcgca taacgcgaga aagagctggc ggccgggtta
    27961 tgtaccgaca tttcccccag cgcacgcata atgatatacg ccgcgatacc accgataata
    28021 taggccaaca acacgctcgg accggccatt ttgatggcgt ctgccgaacc gtaaaacagc
    28081 ccggtgccaa ttgctgaacc cagtgccata aagcgtatgt ggcgggtact tagcccacgc
    28141 tttagcttat tcttactttc catcaattcc taacccatca acacaataaa aaaccacggg
    28201 gcatcgcagc cccgtggtta aaacaaatgt tcagtgattt agtgagcgct ggaggtcacc
    28261 tgacgacctg ccgcgcgatc ccagataatg gccagaacca ccatcaccac tgttggcatt
    28321 aaccacgcca gaccttgttc ggccagcggt aaacgctgcg cccaggacgg taagatatcg
    28381 ctgaatgcag atgctttgat cccgtcgaga ataccaaaaa gcaggctgat aaacatcggc
    28441 ggagcaatca cgcgggacga attatgccac catgagcgtg taaaacttaa tacaaccagt
    28501 gcgatacacg gcggataaat agcggtcagc accggtacgg agatctggat cagctggctt
    28561 aagccgaggt tagacaccac catcgagaag ccgccgagga taaacaccag cgtacgataa
    28621 gagagcggta cgtactgggc aaagaattct gcacaagcac aggtcaggcc aaccgccgtg
    28681 accagacagg cgatgaagat taacgccgcc agcaggaagc taccgccgcc gccaaaggtg
    28741 tgctgaacgt aagcatgcag aatagcagcg ccgtttgcag actgatcgac cagcgacgcg
    28801 ctgtctgaac ccagacagaa cagcgccagg tacagcaaag tcagaccaac acccgccatc
    28861 aggccagccc agacggtata acgggtcagc agacgcgctt cggtaacgcc acgagaacgc
    28921 gccgcgttaa caataacgat accaaacacc attgcgccca gcgtatccat ggtcagatag
    28981 ccgttaacga agccgttaga aaatgcagcg ttttgataag cctcagtcgc cgtgctgata
    29041 gaacccgccg gccagacaat cgcgacaaca gacaggatga ccagcgcgat aattttcagc
    29101 ggcgcaagga agttgcccac ggtatccagc agcttgcccg gatagagcga aaccagaata
    29161 acgatagcga aatagaccag gctgtagata aacagcggca gcgcggaatc acccgtcagc
    29221 ggcgcaatcc ccacttcaaa ggaaacggta gctgtacgcg gcgtagcgaa aagcggcccc
    29281 accgccaggt aacaaaccgt tgccagcagt acgccagcga ctttaccgat tggcgtgctg
    29341 aggctgtcaa caccgccgcc aacttttgcc agcgccacta ccgttaatac cggcaggcca
    29401 acggcagtaa tgaggaagcc gaatgccgca gtccagacgt gttcgcctgc ctgtaagccg
    29461 accattggag ggaaaataat gttacctgcg ccgacgaaca acgcaaatgt cataaagccc
    29521 agagcgatga tatcgcgcga tcttaattga tgggtcataa atcatactgc ctgtggatgt
    29581 ggtgttgtaa cgttaaattt ttgtgccaat ccctggggat gatacagcgg tatatttaaa
    29641 caattttagc gggaaatact cccaaccagg cgcggcgaag aatagttttt cgatttaccg
    29701 cgttaatgca gtcagtatga cagcacctga agcgcaattt aaacgcttat aacgttttaa
    29761 agcaaggagg gatctaaaaa ccagaggtat atagcgataa gaaatcacca accagtataa
    29821 aacactattt ccgtgaaaaa gacatggcta atcatgcgaa caaaaaatca ccaaccgttg
    29881 taaaatctaa cgcccgtatt ttgactgcaa agaaaataag ccagcatcgg ctggcttatg
    29941 gcaataaaag atgacaaagg cggattaatc gctgtttttg gcaattaaac gttccgggat
    30001 aacaaaactg aaacgtgttc cttttcctac tgtactctca atattcaggc gactttcgtg
    30061 atgattcacc gcatgtttca cgatcgctaa ccctaatccg ctaccgccgg tttgccggga
    30121 acgcgcttta tcaacgcgat aaaaacgctc ggtcaggcgc ggaatatgct ccggtgcaat
    30181 gcccggtccg ttatcttcaa cgctaaattc ggcaccgtgc ggcactcgct gccagcgtac
    30241 ggtgatatgc gtgccttccg gcgtatgatt cacggcgtta tagaccaggt tcgaaatcgc
    30301 actgcgtagc tgatcttcgt tgccagacac cttgaggccg ttatctatct caaaggtaaa
    30361 tgtctgtttt ttctgactca gagtctgagc ctcgcgctca acaacgcgca gcatcatcgg
    30421 cacatcaacc ttttcattga gcaattgcgt cggcgcggct tcaattttcg acagcgttag
    30481 caactgcttc accagccctt ccatccgctg ggtctgctcg cgcatggtgt gcaacgcttt
    30541 ttcgcgtacc gcgccttcca gcggctgctc atccatcatc tccaggtaac cctgtaacac
    30601 ggtcaatggc gtacgtaact catggctcac gttggcaaaa aagttacgcc gcgccccttc
    30661 cagttgatgc atttgcgtga catcacgcgc caccatcagc aactgtttgt gggtataagg
    30721 catgacgcga atttccagat gccgcccggt gttgagcacc agattgagcg ggcgagaaaa
    30781 atcacgcgtt ttcagatatt gcgtaaactc cgggtaacgc agtaggttaa ggatgttctg
    30841 cccgttatct tccggccagc gcaaaccaag aatttgttgc gccagaccgt tacaccagaa
    30901 aataccgccc tcttccgtgg tcagcaccac cgcgtcgggc agcgactccg cgccgctacg
    30961 aaagcgttta atcagattgc ccagttcacg gcggcgtttt ttatttcgca gctgcatctg
    31021 gtgtaagccg tatagtagcg gttcccagct accacgcccc ggtggcgggg tcatactgcg
    31081 atccacccac agccaccatg aaaggtgcaa taaattccag aaatgccaga taagcagtcc
    31141 tgttaccgat gccagcaaaa accagggcag gtaaccaaaa aatgcaccca ggatgaaagc
    31201 cgggaggcag caaagtagca gctccagcac cagccttttc cacgacagcc gttccagcac
    31261 gtaagatact ccagttaaga aatcataagc cctgctctgc gtccgatgag caaggcgtta
    31321 aaagcgggtt gaaaaacgat atcctgtacc gcgcacggtc tgcaccatgc ggtcatgccc
    31381 gccgggctcc agtgctttac gcaggcgacg aatgtggaca tcgaccgtgc ggtcttccac
    31441 atacacgtta gttccccaga cgtggtttaa cagctgctcg cggctgtaca cgcgctcagg
    31501 atgcgtcata aaaaagtgca gcagtttaaa ttctgtcggc cccatctcca gcggctcttc
    31561 gcccgccatc actcggtgag atgtcgggtt gagacttaat ccctgcatct taatcacctc
    31621 ttccaccgcc attggcgaaa tacggcgcat taccgctttg attcgcgcca ccagctcctt
    31681 cggcgaaaat ggcttggtga tatagtcatc cgcgccggtt tcaaggccgc gcacgcgatc
    31741 ttcttcttcc cctctggcgg tcaacatcac cactggaata tcccgggtca tcgactcgcg
    31801 cttgaggtgt ttgatgaact ggataccgga gccgccaggt aacatccagt caaggagaat
    31861 taaatccggc cagggttcat tcagttgatt cacagcactg tcataatctt ccgcttcgac
    31921 cggctgaaag ccattttgtt cgagcacgaa gcagaccatt tcgcgaattg gagcttcatc
    31981 ttctacgacc agaatacgtc tcgccatgat ttgccctgtt gtaataaata ggttgcgatc
    32041 attaatgcga cgtcattatg cgtcagattt atgacagatt tatgaaaagc tcgtcgcaca
    32101 tatcttcagg ttattgattt ccgtggcgca gaaaaaagca aatggcacat ctgtttgggt
    32161 ataatcgcga ccatgctttt tcgccaggga accgttatgc gcatccttca cacctcagac
    32221 tggcatctcg gccagaactt ctacagtaaa agccgcgaag ctgaacatca ggcttttctt
    32281 gactggctgc tggagacagc acaaacccat caggtggatg cgattattgt tgctggtgat
    32341 gttttcgata ccggctcgcc gcccagttac gcccgcacgt tatacaaccg ttttgttgtc
    32401 aatttacagc aaactggctg tcatctggtg gtactggcag gaaaccatga ctcggtcgcc
    32461 acgctgaatg aatcgcgcga tatcatggcg ttcctcaata caaccgtggt cgccagcgcc
    32521 ggacatgcgc cgcaaatctt gcctcgtcgc gacgggacgc caggcgcagt gctgtgcccc
    32581 attccgtttt tacgcccgcg tgacattatt tccagccagg cggggcttaa cggtattgaa
    32641 aaacagcagc atttactggc agcgattacc gattattacc aacaacacta tgccgatgcc
    32701 tgcaaactgc gcggcgatca gcctctgccc atcatcgcca cgggacattt aacgaccgtg
    32761 ggttccagta aaagtgacgc cgtgcgtgac atttatattg gcacgctgga cgcgtttccg
    32821 gcacaaaact ttccaccagc cgactacatc gcgctcgggc atattcaccg cgcacagatt
    32881 attggcggca tggaacatgt tcgctattgc ggttccccca ttccactgag ttttgatgaa
    32941 tgcggtaaga gtaaatatgt ccatctggtg acattttcaa acggcaaatt agagagcgtg
    33001 gaaaacctga acgtaccggt aactcaaccc atggcggtgc tgaaaggcga tctggcgtcg
    33061 attaccgcac agctggaaca gtggcgcgat gtatcgcagg agccacctgt ctggctggat
    33121 atcgaaatca ctactgatga gtatctgcat gatattcagc gcaaaatcca ggcattaacc
    33181 gaatcattgc ctgtcgaagt attgctggta cgtcggagtc gtgaacagcg cgagcgtgtg
    33241 ttagccagcc aacagcgtga aaccctcagc gaactcagcg tcgaagaggt gttcaatcgc
    33301 cgtctggcac tggaagacct ggatgaatcg cagcagcaac gtctgcagca tcttttcacc
    33361 acgacgttgc gtaccctcgc cggagaacac gaagcatgaa aattctcagc ctgcgcctga
    33421 aaaacctgaa ctcattaaaa ggcgaatgga agattgattt cacccgcgag ccgttcgcca
    33481 gcaacgggct gtttgccatt accggcccaa ccggtgcggg gaaaaccacc ctgctggacg
    33541 ccatttgtct ggcgctgtat cacgaaactc cgcgtctctc taacgtttca caatcgcaaa
    33601 atgatctcat gacccgcgat accgctgaat gtctggcgga ggtggagttt gaagtgaaag
    33661 gtgaagcgta tcgcgcgttc tggagccaga atcgggcgcg taaccagccg gacggtaatt
    33721 tgcaggtgcc tcgcgtagag ctggcgcgct gcgccgacgg caaaattctc gccgacaaag
    33781 tgaaagataa gctggaactg acagcgacgt taaccgggct ggattacggg cgcttcaccc
    33841 gttcgatgct gctttcgcag gggcaatttg ctgccttcct gaatgccaaa cccaaagaac
    33901 gcgcggaatt gctcgaggag ttaaccggca ctgaaatcta cgggcaaatc tcggcgatgg
    33961 tttttgagca gcacaaatcg gcccgcactg agctggagaa gctgcaagcg caggccagcg
    34021 gcgtcacgtt gctcacgccg gaacaagtgc aatcgctgac agcgagtttg caggtactta
    34081 ctgacgaaga aaaacagtta attaccgcgc agcagcaaga acaacaatcg ctaaactggt
    34141 taacgcgtca ggacgaattg cagcaagaag ccagccgccg tcaacaggcc ttgcaacagg
    34201 cgttagccga agaagaaaaa gcgcaacctc aactggcggc gcttagtctg gcacaaccgg
    34261 cacgaaatct tcgtccacac tgggaacgca tcgcagaaca cagcgcggcg ctggcgcata
    34321 ttcgccagca gattgaagaa gtaaatactc gcttacagag cacaatggcg cttcgcgcga
    34381 gcattcgcca ccacgcggcg aagcagtcag cagaattaca gcagcagcaa caaagcctga
    34441 atacctggtt acaggaacac gaccgcttcc gtcagtggaa caacgaactg gcgggttggc
    34501 gtgcgcagtt ctcccaacaa accagcgatc gcgagcatct gcggcaatgg cagcaacagt
    34561 taacccatgc tgagcaaaaa cttaatgcgc ttgcggcgat cacgttgacg ttaaccgccg
    34621 atgaagttgc taccgccctg gcgcaacatg ccgagcaacg cccactgcgt cagcgcctgg
    34681 tcgcgctgca tgggcagatt gttccccaac aaaaacgtct ggcgcagtta atggtcacta
    34741 tccagaatgt cactctggaa cagacgcaac gtaatgtcgc acttaacgaa atgcgccagc
    34801 gttataaaga aaagacgcag caacttgccg atgtgaaaac catttgcgag caggaagcgc
    34861 gcatcaaaac gctggaagcc cagcgtgcac agttacaggc gggtcagcct tgtccacttt
    34921 gtggttccac cagccacccg gcggtcgagg cgtatcaggc gctggagcct ggtgttaatc
    34981 agtctcgatt actggcgctg gaaaacgaag ttaaaaagct cggcgaagaa ggtgcggcgc
    35041 tgcgcgggca actggatgca ttaacgaagc agcttcagcg cgatgaaaac gaagcgcaaa
    35101 gcctccgaca agatgagcaa gcacttactc aacaatggca agccgtcacg gccagcctca
    35161 atatcacctt gcagccacag gacgatattc aaccgtggct ggatgcacaa gatgagcacg
    35221 aacgccagct gcggttactc agccaacgac atgaattaca agggcagatt gccgcgcata
    35281 atcagcaaat tatccagtat caacagcaaa ttgaacaacg tcagcagcag ctcttaacgg
    35341 cattggcggg ttatgcactg acattgccac aggaagatga agaagagagc tggttggcga
    35401 cacgtcagca agaagcgcag agctggcagc aacgccagaa cgaattaacc gcgctgcaaa
    35461 accgtattca gcagctgaca ccgattctgg aaacgttgcc gcaaagtgat gatctcccgc
    35521 acagcgaaga aactgtggcg ctggataact ggcggcaggt tcatgaacaa tgtctcgcat
    35581 tacacagcca gcagcagacg ttacagcaac aggatgttct ggcggcgcaa agtctgcaaa
    35641 aagcccaagc gcagtttgac accgcgctac aggccagcgt ctttgacgat cagcaggcgt
    35701 tccttgcggc gctaatggat gaacaaacac taacgcagct ggaacagctc aagcagaatc
    35761 tggaaaacca gcgccgtcag gcgcaaacgc tggtcactca gacagcagaa acgctggcac
    35821 agcatcaaca acaccgacct gacgggttgg ctctcactgt gacggtggag cagattcagc
    35881 aagagttagc gcaaactcac caaaagttgc gtgaaaacac cacgagtcaa ggcgagattc
    35941 gccagcagct gaagcaggat gcagataacc gtcagcaaca acaaacctta atgcagcaaa
    36001 ttgctcaaat gacgcagcag gttgaggact ggggatatct gaattcgcta ataggttcca
    36061 aagagggcga taaattccgc aagtttgccc aggggctgac gctggataat ttagtccatc
    36121 tcgctaatca gcaacttacc cggctgcacg tgcgctatct gttacagcgc aaagccagcg
    36181 aggcgctgga agtcgaggtt gttgatacct ggcaggcaga tgcggtacgc gatacccgta
    36241 ccctttccgg cggcgaaagt ttcctcgtta gtctggcgct ggcgctggcg ctttcggatc
    36301 tggtcagcca taaaacacgt attgactcgc tgttccttga tgaaggtttt ggcacgctgg
    36361 atagcgaaac gctggatacc gcccttgatg cgctggatgc cctgaacgcc agtggcaaaa
    36421 ccatcggtgt gattagccac gtagaagcga tgaaagagcg tattccggtg cagatcaaag
    36481 tgaaaaagat caacggcctg ggctacagca aactggaaag tacgtttgca gtgaaataac
    36541 tattcagcag gataatgaat acagaggggt gaattatctc ttggccttgc tggtcgttat
    36601 cctgcaagct atcactttat tggctacggt gattggtagc cgttctggtg gttgtgatgg
    36661 tggtatgaaa aaagtcattt tatctttggc tctgggcacg tttggtttgg ggatggccga
    36721 atttggcatt atgagcgtgc tcacggagct ggcgcataac gtaggaattt cgattcctgc
    36781 tgccgggcat atgatctcgt attatgcact ggtggtggtg gtcggtgcgc caatcatcgc
    36841 actcttttcc agccgctact cactcaaaca tatcttgttg tttctggtgg cgttgtgcgt
    36901 cattggcaac gccatgttca cgctctcttc gtcttacctg atgctcgcca ttggtcggct
    36961 ggtatccggc tttccgcatg gcgcattttt tggcgtcgga gcgatcgtgt tatcaaaaat
    37021 tatcaaaccc ggaaaagtca ccgccgccgt ggcggggatg gtttccggga tgacagtcgc
    37081 caatttgctg ggcattccgc tgggaacgta tttaagtcag gaatgctggc gttacacctt
    37141 tttattgatc gctgttttta atattgcggt gatggcatcg gtctattttt gggtgccaga
    37201 tattcgcgac gaggcgaaag gaaatctgcg cgaacaattt cactttttgc gcagcccggc
    37261 cccgtggtta attttcgccg ccacgatgtt tggcaacgca ggtgtgtttg cctggttcag
    37321 ctacgtaaag ccatacatga tgtttatttc cggtttttcg gaaacggcga tgacctttat
    37381 tatgatgtta gttgggctag ggatggtgct gggaaatatg ctaagtggca ggatttcagg
    37441 acgttattca ccactgcgca ttgcagcagt gactgacttt atcattgtac tggcactgct
    37501 gatgctcttt ttctgcggcg gcatgaaaac aacgtcgctt atttttgctt ttatttgttg
    37561 cgcgggatta tttgcccttt cagcacctct gcaaatattg ttactgcaaa atgccaaagg
    37621 aggagagtta ttaggtgccg caggtgggca aatagcgttt aacctcggta gcgccgtcgg
    37681 cgcatattgc ggaggtatga tgctgacgct ggggctggca tataattacg tggcgctgcc
    37741 tgccgccctg ctttcgtttg ctgcgatgtc atcgttgctg ctgtatggtc gctataagcg
    37801 ccagcaagcg gcggatagtc cggtgctggc gaaaccactg gggtagatta ctgtcacggt
    37861 gcgcgttgct tattgccgga tgcggcgtga acgccttatc cgccctacgc ggttctggca
    37921 cattttgtag gcctgataag acgcggcaag cgtcgcatca ggcatgatgc gccaattgcc
    37981 tacgtttttt actcttgtgg ccataaccac gcagcgccgc gtacgccgct ggaatcaccg
    38041 tgcttcgcct tactcaccgg cgtttcacat tcgccgccga agacaaattg tttaatcaac
    38101 tgcccaaccg tttgatataa acggtctaca ttgctcatcc cgccccccag gacaatcaca
    38161 tccggatcga gaatattcac gacatgtgcc agcgattttg ccagccgcag ctcgtagcga
    38221 cgcaatgcca gttccgctac cggatcgctt tcttcaacca ggcggataat ttcactgcct
    38281 ttcagcgcat gtccgctcaa acgacgataa tccgtcgcga atcccgtgcc cgaaataaag
    38341 gtttcaatac aaccttgttt accgcaataa caagggactt cctcgcgata acgcagttcg
    38401 tcttcgtcca tccacggtag cggattgtgt ccccactcac ctgccgtgcc attgccgccg
    38461 atatgcgtcc gcccattgaa tgccacgccc gcgccgcatc ccgtgccgat aatcacggca
    38521 aataccgtct gcgctcccgc tgccgcgcca tctactgctt ctgaaaccgc cagacagttg
    38581 gcatcatttg ccagccgcac ttcccgctgc aacctcgcgc ttaagtcttt atcgaatggc
    38641 tgaccgttga gccaggctga attgacattc ttcaccacac cggtgtaggg cgaaatcgaa
    38701 cctggaatgc ccatacctac cgttccgcgc tgccctgtcg cctgctccgc catatcaacc
    38761 aacgtggcga tcgtttcaat agtctgccgg taatcatcac gcggcgtggg cagacgatgg
    38821 cggtacaact gctcccctgc atcgcccagt gcaatcactt cagttttggt gccgcctaaa
    38881 tcgataccta tacgcactgt actctcctta tttttttcaa tatcaatagc gtagagacgg
    38941 acaaccggat tggcaatgca aggccgccga caattcgtta tcatgcccgc taaatttaac
    39001 gacaaggccg tggaaattat catgctgtgg ttcaaaaatt taatggttta ccgtcttagc
    39061 cgcgagattt cgctgcgtgc agaagagatg gaaaaacagc tagcctcgat ggcatttacc
    39121 ccatgcggca gccaggacat ggcgaagatg ggctgggttc ctccgatggg atcgcacagc
    39181 gatgcgttaa cgcacgttgc caatggtcaa attgttatct gcgcgcgcaa agaagaaaaa
    39241 atcctcccgt ctccggtgat taaacaggcg ctggaagcga aaatcgccaa actggaagcg
    39301 gaacaggcgc gtaagctgaa gaaaaccgaa aaagattcgc tgaaagacga agtgctgcac
    39361 tctctgctgc cgcgtgcttt cagccgtttt agccagacaa tgatgtggct cgacacggtt
    39421 aacggtttga ttatggtgga ctgcgccagt gccaaaaaag cggaagatac gctggcatta
    39481 ctgcgtaaaa gcctggggtc gttaccggtt gtaccattga gcatggaaaa cccgattgaa
    39541 ctgacgctga ccgaatgggt tcgctccggt agtgcggcac agggcttcca gctgcttgat
    39601 gaagccgagc tgaaatcgtt gctggaagat ggcggcgtga tccgcgcgaa gaaacaagat
    39661 ctgaccagcg aagagatcac caatcacatt gaagccggaa aagtggtgac taaactggcg
    39721 ctcgactggc agcagcgcat tcagtttgtg atgtgcgacg atggttcgct caagcgtctt
    39781 aagttctgcg acgagctgcg cgatcaaaac gaagatatcg accgtgaaga tttcgcccag
    39841 cgttttgatg ccgatttcat cctgatgact ggtgaactgg cagcgttaat tcaaaacctg
    39901 attgaaggat taggtggcga agcacaacgt taattgctga ttttccttta atgccggatg
    39961 cgacgcctgc cgcgtcttat ccggcgtacg aagccacacc aggcatataa ttattcgcta
    40021 cggcgagcaa taatttttag cgcagcaata ttatgcgttt tacgccgtaa ctgatctaat
    40081 cgcctcatcc ctattcgcct gtcagcagca cgcagaatat tcactaaccc ccatttatcg
    40141 ctggtaaatt gttttgttaa cagctccgct tttggggtat caatatattc acgcaataaa
    40201 tcagaaattg aacaaatatc cgtcaggtaa ggataccagc aagtttcacc gtgaaaatta
    40261 cgcactccag cgttacctgc aataaagtct ttcgggtaat cccagatcac gtttttatcc
    40321 aacgtgatcc aataacgcgg taaatcggta cttccaccat tttggctgcg catgggataa
    40381 cgggtacagt gaatttgcag gttgatcgtt ggcgtcagta agtcatagat ctcccgctgt
    40441 aacttgctcc atggacgttg tgtcattgtt tttcctcaag ccggtgacgc gcttcactgc
    40501 gaattttccg cgttgccccg gttttcacta aatcacgtaa ctgcgaaaga tcgggttgtt
    40561 cgacaaataa gagtaatgat ggatcctctt ttaaccacgc cgttttcaca tcggcagcca
    40621 gctgtggatt attaatagcc agcgaccgat cctgaggctc agtcaatgtc gttaacaacg
    40681 acgccggggt atgtgcgttg cgtaatgccg tttggcgcaa atgatcattg cgactttgca
    40741 gcaatgcacg ccaccattca tcatctgatt tatcttttat cgtagccagt tccgcaaaaa
    40801 atacctggag atcttcactg atatttatat caaccttgat gaattatgaa tatatcggaa
    40861 attgaaaaaa tcgcagcaaa ggaaatatta ttatgggtta aagagaggat atttcagaaa
    40921 aatgccctcc cgttcaaggg gaaggcgagg aattacagat agcggcacag ataagaggta
    40981 ggttcggcaa cttgaagatg aaactcactg tgaccgggaa cattaaacac cgaaccggct
    41041 tcatacactt gccagtcggt cgcgtcaggc agtaacacat tcagcgcgcc actgattacc
    41101 gtcatctctt ccggctcagc ggtgctgaag gtgtattcgc cttcaaccat aacacccacg
    41161 ctggcgcgac cagtgctgct gctggaaaag ccgatcgatt tcactttgcc ggaaaagtac
    41221 tcattacttt gaagcataaa ctggccctgt ctggtagttg aacatctact tcactatagg
    41281 ggccagaggc gctggctgtc acgcaaaatt acaccagtaa ttccgcagcc agccgtgcaa
    41341 tcaatacgtt agagagcaag acaggaacat cgagctgttt ttgcagtaaa tcgcgatgac
    41401 gttggtgaaa tcctaaacaa tccagcatga tgacatcagc cccttttgcc agtaattctt
    41461 tcccagcatc aatgattttt tgttctgaat catgaatggg gttacccaat gaaaataccg
    41521 gcgatttctg caaaatttgc catttttgcg cctgaacggg cagcatctcc tcaaccggaa
    41581 cgataacccc cacctgatga tcttcaacaa tagaggaaac cagtggaggc aatattcgcg
    41641 acggctcaag aaagatcgta ttacgcgcag tcatactact aatgtttgct gtactcatta
    41701 ataaaatgac gtcataaccc tgattatcga gcacttcaac cacaccttgc agatctcgct
    41761 ccactttacg acgcgaaaca tgggccagct ggttgtcatt taataatgtg agaatggtgt
    41821 cttcgcctgc ttctggcgcg tactccgcca tcacttcttc acgacttaac ttccccagca
    41881 ggctatgatg ggaaatatta tcttcgtcaa tgtattccgt caggagcggc aaaacctcct
    41941 gcataggtac aatgccgatg gtcaggatcg ccaacgacgc actcattgtt catccacctt
    42001 acttttcttt cgtcgttaat taccgggcaa gtgtgaagca ccactgctga cacttacttg
    42061 tgctacaaat gacaaaaagc gtagcagcag acgtgcacgg cataatgtaa agattccaaa
    42121 tgattccagt aatggaatgt tattgtttaa tattctaatt attagaaaaa cataaattat
    42181 gaaaaatgtg acgcagatcc cttgtagtgg cactcagaat cccttccgag cagtctgatg
    42241 cggtcactct ttgtcagggt cctcatagcg ttttttcgca tccatcgctt cctgagccgt
    42301 cggatgttca ctgatcaacg agtctggttt aggatgatcg gctctgagtt ggtaccaggt
    42361 taccgtctgt cctggctttc ctttttcaat cgtcactata tatgcttcac gaggataagg
    42421 cggtttcgtt ggcatcgttc ttccttattt cacgggatga acgttaagta taggcgctct
    42481 aaaatcaaca attgatcgtc tgtgccaggg cgctacgaat ttcagaaatc acctggctgg
    42541 gttcgtttgt tgcgtcgatg ataatatgcg caacttcgcg atatagcgca tcgcgttctt
    42601 ccagcacttc ctgaacttct tcgctcagcg gttttcccgt taaggttggc cgtaaatctt
    42661 cttccggtgc agcttgcagt cggttaacca ggactgatac tggcgcacac aaataaacca
    42721 cgatcccgtt attttgcatg aagtgacgat taaattccgt cagaataatg ccgccgcctg
    42781 tagcgataac ggtggatgcc gcagttaccg cttccagcgc cgccgtttct ctggcgcgaa
    42841 atcccgccca ctcttccctt tcgacgatct ccgcgaccgt catattgagc tgtgattgca
    42901 accactgatc ggtatcgaca aaccgacggt taagcgaatc ggcaagggcc attccgaccg
    42961 ttgttttacc acagccccga ggcccgatca gaaaaagagg ttgtgtcatc gtgggttttc
    43021 cccaataggt cgcaatgcgg cgaaagccgg tgtcatgaga atagcgatca taccatcaaa
    43081 ctagtacaat ttcgattgta aagaaaaaat tccacttaaa gtaaaaatct caatacatcc
    43141 cttactatac caataaatat tcaagaatga agtgtaaata ataaattaca tttagccacg
    43201 actacgttgc acttccagcc accacttctc cagctccgcg gcaaaggcct ggcggtcacg
    43261 ttgtgaaagg ctatctggtc cgccggtctg gatcccactg gcacgtaagg tatccataaa
    43321 atcgcgcatc gtcaggcgct cacgaatggt cgctggcgtg taacgttcgc cgcgcgaatt
    43381 aagcgccgct gcgcctttct cgatggcttc agcagccaaa ggtatatctg cggtgatcac
    43441 caaatcgccc gcttcacact gccggacaat ttcgttatcg gcaacgtcga aacctgccgc
    43501 gacgcgcagc gtacgaataa atcgcgatgg cggcacgcgt aaactctggt ttgctaccag
    43561 taccagcggc atctgcatac gttccgccgc gcgatacaaa atctctttaa ttacattggg
    43621 acacgcgtcg gcatccaccc aaattgtcat aaagtcatcc tttgttaggt aatcctctat
    43681 tgtgtcgcgc ttttgccttc cggcatagtt ctgtttatgc ttctgccagc gattatcaaa
    43741 acaatgaatt tcacggcagg agtgaggcaa tggaaaagaa aatcggtttt attggctgcg
    43801 gcaatatggg aaaagccatt ctcggcggtc tgattgccag cggtcaggtg cttccagggc
    43861 aaatctgggt atacaccccc tccccggata aagtcgccgc cctgcatgac cagttcggca
    43921 tcaacgccgc agaatcggcg caagaagtgg cgcaaatcgc cgacatcatt tttgctgccg
    43981 ttaaacctgg catcatgatt aaagtgctta gcgaaatcac ctccagcctg aataaagact
    44041 ctctggtcgt ttctattgct gcaggtgtca cgctcgacca gcttgcccgc gcgctgggcc
    44101 atgaccggaa aattatccgc gccatgccga acactcccgc gctggttaat gccgggatga
    44161 cctccgtaac gccaaacgcg ctggtaaccc cagaagatac cgctgatgtg ctgaatattt
    44221 tccgctgctt tggcgaagcg gaagtaattg ctgagccgat gatccacccg gtggtcggtg
    44281 tgagcggttc ttcgccagcc tacgtattta tgtttatcga agcgatggcc gacgccgccg
    44341 tgctgggcgg gatgccacgc gcccaggcgt ataaatttgc cgctcaggcg gtaatgggtt
    44401 ccgcaaaaat ggtgctggaa acgggagaac atccgggggc actgaaagat atggtctgct
    44461 caccgggagg caccaccatt gaagcggtac gcgtactgga agagaaaggc ttccgtgccg
    44521 cagtgatcga agcgatgacg aagtgtatgg aaaaatcaga aaaactcagc aaatcctgat
    44581 gactttcgcc ggacgtcagg ccgccacttc ggtgcggtta cgtccggctt tctttgcttt
    44641 gtaaagcgcc aaatctgccg atttcaacca ctcacgatag tgactcattt gtgggttcag
    44701 cggcgcaacc cccacactaa tccgtaaagt tacctgtggc gtattcggca agcgtaacgt
    44761 gtttagccct tcacgcaccc gtaacatggc ggtaatggcg ctctcagctg gcgtaccgga
    44821 catgattact gcaaactcat cgccgccaaa ccgaccaatc acatcgctac cgcgcagggt
    44881 aatttgtaac tgtcgggtaa gcgccacaat cgcttcatcg cccacatcat gaccccaggt
    44941 atcgttgatg ctcttgaaat ggtcgatatc gataatcagt aacgttgcat cgcgattatg
    45001 ccgccgacag ttatcaaatt cattgcgtaa catagtttcc caatgacgtc ggttatacac
    45061 gcccgtcatg ccgtcgcgag tactcatgac ctgcaacctg cgtttatgtt ccgccagttt
    45121 ggttgccgtc tggtagctga cccagccaaa catcagagga taaacgacaa taatgggaag
    45181 ggagagccac cattccagcg gagcactatt gaacgacacg gtaatgcccg taagctcgag
    45241 ggtgacaagg caggaaacca ccatcaacac cagacccgcg acaaacagac gggggccgcc
    45301 tgcccccatc aaattcagac acagaatcat caacatagcg gtggaaggca gcacgtttac
    45361 gcccattacg cctacccaca ttcccgctaa tactgcatcg gtttttaagt tgtaaatttc
    45421 ccggctaagc ggatcgacgg ccctgctcgc tatctgccag gctaaatgcg gccagacgaa
    45481 cgcccagccg accaacacca gccaccacca gcccggcggc gggtgcgaaa ccagcgttga
    45541 agcaatcggt aagaacatgc cagccaggcc aaccgcacgg ggtagtctga cgcgacgggc
    45601 gaagcgcagc ccggaccgct gatgttcgtt ttgaggagtt aaaggaggtt cctccccatg
    45661 cgccgccgct tttttgaaaa agttttcatc attcattatt tttgggaaca ttcagaaaca
    45721 attttcccaa attatagaga cggggctaaa ggcagaagta tgaaatttcg ggtgagccac
    45781 tttcgtagct cacccgttag cact
//
LOCUS       DAJTSA010000026        42790 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000026 DAJTSA010000000
VERSION     DAJTSA010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.026"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OO369_001513"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OO369_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR5981838.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="OO369_001514"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="OO369_001514"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR5981839.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="OO369_001515"
     CDS             complement(3736..4092)
                     /locus_tag="OO369_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5981840.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="OO369_001516"
     CDS             complement(4096..4512)
                     /locus_tag="OO369_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR5981841.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OO369_001517"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OO369_001517"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR5981842.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5942
                     /locus_tag="OO369_001518"
                     /pseudo
     CDS             5738..5942
                     /locus_tag="OO369_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="OO369_001519"
     CDS             complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="OO369_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR5981843.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7891..8970)
                     /gene="alr"
                     /locus_tag="OO369_001520"
     CDS             complement(7891..8970)
                     /gene="alr"
                     /locus_tag="OO369_001520"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR5981844.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="OO369_001521"
     CDS             complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="OO369_001521"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5981845.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10521..11504
                     /locus_tag="OO369_001522"
     CDS             10521..11504
                     /locus_tag="OO369_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR5981846.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="OO369_001523"
     CDS             complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="OO369_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR5981847.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="OO369_001524"
     CDS             complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="OO369_001524"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR5981848.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13446..14431)
                     /locus_tag="OO369_001525"
                     /pseudo
     CDS             complement(13446..14431)
                     /locus_tag="OO369_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14749..15264
                     /gene="zur"
                     /locus_tag="OO369_001526"
     CDS             14749..15264
                     /gene="zur"
                     /locus_tag="OO369_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR5981849.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15306..15515)
                     /locus_tag="OO369_001527"
     CDS             complement(15306..15515)
                     /locus_tag="OO369_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR5981850.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="OO369_001528"
     CDS             complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="OO369_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR5981851.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="OO369_001529"
     CDS             complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="OO369_001529"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR5981852.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="OO369_001530"
     CDS             complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="OO369_001530"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR5981853.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18286..20709
                     /gene="plsB"
                     /locus_tag="OO369_001531"
     CDS             18286..20709
                     /gene="plsB"
                     /locus_tag="OO369_001531"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR5981854.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="OO369_001532"
     CDS             complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="OO369_001532"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR5981855.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="OO369_001533"
     CDS             complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="OO369_001533"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR5981856.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22469..24049)
                     /locus_tag="OO369_001534"
                     /pseudo
     CDS             complement(22469..24049)
                     /locus_tag="OO369_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24278..25198)
                     /gene="malM"
                     /locus_tag="OO369_001535"
     CDS             complement(24278..25198)
                     /gene="malM"
                     /locus_tag="OO369_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR5981857.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="OO369_001536"
     CDS             complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="OO369_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR5981858.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26762..27877)
                     /gene="malK"
                     /locus_tag="OO369_001537"
     CDS             complement(26762..27877)
                     /gene="malK"
                     /locus_tag="OO369_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR5981859.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28242..29432
                     /gene="malE"
                     /locus_tag="OO369_001538"
     CDS             28242..29432
                     /gene="malE"
                     /locus_tag="OO369_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR5981860.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29586..31130
                     /gene="malF"
                     /locus_tag="OO369_001539"
     CDS             29586..31130
                     /gene="malF"
                     /locus_tag="OO369_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR5981861.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31145..32035
                     /gene="malG"
                     /locus_tag="OO369_001540"
     CDS             31145..32035
                     /gene="malG"
                     /locus_tag="OO369_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR5981862.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            32406..33881
                     /gene="xylE"
                     /locus_tag="OO369_001541"
     CDS             32406..33881
                     /gene="xylE"
                     /locus_tag="OO369_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR5981863.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(33925..34335)
                     /gene="psiE"
                     /locus_tag="OO369_001542"
     CDS             complement(33925..34335)
                     /gene="psiE"
                     /locus_tag="OO369_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR5981864.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(34461..34604)
                     /locus_tag="OO369_001543"
     CDS             complement(34461..34604)
                     /locus_tag="OO369_001543"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981865.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            34605..34883
                     /locus_tag="OO369_001544"
     CDS             34605..34883
                     /locus_tag="OO369_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981866.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(34930..37026)
                     /locus_tag="OO369_001545"
     CDS             complement(34930..37026)
                     /locus_tag="OO369_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5981867.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(37026..37763)
                     /locus_tag="OO369_001546"
     CDS             complement(37026..37763)
                     /locus_tag="OO369_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5981868.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(37760..38398)
                     /locus_tag="OO369_001547"
     CDS             complement(37760..38398)
                     /locus_tag="OO369_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5981869.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(38512..38754)
                     /locus_tag="OO369_001548"
     CDS             complement(38512..38754)
                     /locus_tag="OO369_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981870.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(39217..40866)
                     /gene="pgi"
                     /locus_tag="OO369_001549"
     CDS             complement(39217..40866)
                     /gene="pgi"
                     /locus_tag="OO369_001549"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR5981871.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            41391..42740
                     /gene="lysC"
                     /locus_tag="OO369_001550"
     CDS             41391..42740
                     /gene="lysC"
                     /locus_tag="OO369_001550"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR5981872.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
BASE COUNT        10642 a        11014 c        10504 g        10630 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag
     5881 acctggagaa gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt
     5941 agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc
     6001 cgaggaggtt aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca
     6061 atataaggtt aaacttacac aaagcggaaa aaagagttct ctttaacatt agaatttaac
     6121 gatactataa ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttgaggat
     6181 atagaaatat tctatcgtaa caaacccgat acttattatt aaatttaata atggttagat
     6241 gtgcagaaat tattactcca cacgaaaaaa tagggaaagg aattccagat aattgggata
     6301 aacttaacaa taaaagaact gacaacgttt tattatctct cacgtagaac aacggcatca
     6361 aaaaaatcat ctacaaagaa atagtgaaag cgacgacagt ttaatttcac tgcaggctgg
     6421 gtaactccag cctgctttcc tgcattacat caccgcagca aacgcctttg ccacacgttg
     6481 tacatttgcc gtatttaacc cggcgacaca catgcgaccg ctggcgatga gatagacacc
     6541 aaattcttca cgtagtcggt caacctgagc ggcacttaaa ccggtataac tgaacatgcc
     6601 gcgctgatta agcagataat cgaaattgcg ttctggcatc tctgtgctta ataccttcac
     6661 caattcctga cgcattgcca gaatgcgagt acgcatctct tctacttccg ccagccagct
     6721 ggctttcaat gcctcgtcat tcagcactgc agccaccacc tgcgcaccaa aattcggcgg
     6781 gctggagtag ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc cagcggcttc
     6841 ggcatcttca cacagaacag aaagtccgcc gacgcgctcg ccgtaaaggg agaaaatttt
     6901 cgagaacgaa ttgctcacca gagcgggtaa tccagcactg gcaatggcgc gaatagcgta
     6961 ggcatcctct tccataccgg cactaaatcc ttgataggca atatcgagga atgggataag
     7021 ctcgcgggct ttgagaattt caatcaccgc atcccactga tcattagtga gatcggcacc
     7081 cgttgggttg tggcaacatg gatgcagcaa cacaatactg cgggcaggta atgttttcag
     7141 cgtcgccaac aggtcattaa agcgcacgct gttagtcact tcgtcatacc aggggtaagt
     7201 actcacttcg aatccagccc cggcgaatat tgctacgtgg ttttcccaag taggatcgct
     7261 gacccagacg cctgattccg ggaagtagcg tttcaggaaa tccgcgccca ctttcaatgc
     7321 ccctgagccg ccaagggttt gaatggttgc tacgcgctgt tgttgcagta ccggatggtc
     7381 cgcaccaaac agcagcggcg caatggcatg gcgatagctg ttaagccctt ccatcggtaa
     7441 ataaagcgaa gcgccatggg gctgcgcatt caggcgcgct tccgcctccg ccacggcttt
     7501 caattgtgga ataattccgt cttcgttgta gtacagaccg atacttaaat tcactttgtc
     7561 gctgcgaggg tcttctttaa aacgctccat aagcgtaaga atcgggtcgc cagcgtaggc
     7621 gtcaactttt tgaaacacgc gatggttctc caggtttacg ggcaggtggt taaaacacaa
     7681 taaaccggaa gaaggcgaag atcgagtgga tgttcaggag cgaacggcaa ttagcaacag
     7741 agtgagacta atgataaacg tacatccgcc agaggcacga cctttataag agcatgaaag
     7801 aacatcaact tattgaattt ttaggatttt attggccgga taaggcattc acgccgcatc
     7861 cggcacagac aatcaaatat tacagaacga ttaatccacg tatttcatcg cgacccttga
     7921 agtcaggcgc gtaataagtt cgtaagcgct tactttcgtc atttcagcga tacgttctac
     7981 gggcaaacct tcgccccata aaatgaccgg atccccggct ttgtcctgcg cctgtggacc
     8041 taagtctacg cagatcatat ccatcgccac gcgcccgaca atcggtactt cgcgaccgtt
     8101 caccagcact ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat agcccatcgc
     8161 gactacgcca agacgggtat cacgttcgct tacccaggtt ccaccataac caacaggctc
     8221 tccggcttta tgctcacgca cggcaatcag gctggaggtt agtgacatca ctggctgaca
     8281 gccaaaatcg gcaccggtgg agcgatcttc cagcggcgag acgccataaa gaatgatgcc
     8341 cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga atgccacccg acgcggcaat
     8401 ggaacgttga ccaggtttgc cttcgcaaaa ggtattaaag atagcgagtt gtttctcggt
     8461 tgcgccacat tttggttcat ccgcgcgcgc aaaatggctg acgatattca ccggctgacg
     8521 aacgtttttg cactgggtca ggcgatgata aaacgcctca gcctgttccg gccttacgcc
     8581 cagacggtgc ataccggtat cgagtttcat ccagacggta accggctcgt ccaagctagc
     8641 ctcttccagc gcagccagct gttcttcgtt atgcacggcg gtatgaaaat gttgcgcaga
     8701 aatcgtcggc agatctctgg catcaaaaaa gccttcgagt aacagtacag gtttggtgat
     8761 tccccccgca cgcagtcgca gagcttcttc gagacgggct acgccaaagg cgtcagcatc
     8821 ggggagcgtt cgcgcggtct caagaagacc gtgaccataa gcgttcgctt tcaccaccgc
     8881 aaccatttta ctggcaggtg ccagttcacg aagacgttgc aggttgtgtc gcagagcgcg
     8941 gcggttaatc acaacagttg ccgcttgcat ttgtgttcct tgataattgt ttgctttaat
     9001 tacctaattc ataaaataat tattattcgt cgtcgtactg cggccccgca tagttgtcga
     9061 agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg ttacgttgtt
     9121 taccgataat aatttccgcg atgcctttta aatcactgtt ttcgtgatac acctcatcac
     9181 gatagataaa catgatcaag tccgcatcct gctcgataga gccagattca cgcaggtcgg
     9241 agttgaccgg acgtttgtcg gcacgttgtt ccagagaacg gttcaactgg gacagcgcca
     9301 ccaccggcac gttcagttct ttcgccagtg ctttcagcga gcgggagatt tctgcaattt
     9361 ccagcgtacg gttatcggaa agcgccggta cgcgcatcag ttgcaggtag tcgatcatga
     9421 taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc acttccgttg
     9481 gcgtcaggcc ggaagagtca tcgatataga tattgcgttt ttcgagcaaa atacccatgg
     9541 tgccggaaat gcgcgcccag tcttcatcat cgagctgccc ggtacggatt ttagtctggt
     9601 caacgcgcga cagcgacgcc agagaacgca tcatgatctg ttctgatggc atctccagcg
     9661 agaagataag tactggttta tcctgcaaca ttgccgcgtt ttcgacgagg ttcatcgcaa
     9721 atgttgtttt acccatcgac ggacgcgcgg cgacgatgat caaatccgac ggctgcaagc
     9781 cagcggtttt tttgttgaga tcgtcataac cggtgtttac cccggtaacg ccatcgtgtg
     9841 gctgctgaaa caactgctca atacgcgcca cggttgcgtc gagcacatcg gcgatgttct
     9901 tcggcccttc gtctttgttt gcacgacttt cggcaatttt aaagacgcgg gattcagcaa
     9961 ggtccagcag atcttcgctg gtacgcccct gcggatcaaa accggcttca gcaatctcat
    10021 tcgcaaccga gatcatctca cggacaacgg cacgttcacg cacgatgtcc gcataagcac
    10081 tgatgttcgc cgcacttggc gtattttttg acagctctgc cagataagca aaaccaccga
    10141 cgctatcgag ttgcccctgg cgttccaacg attccgcaag ggtaatcaga tcgataggac
    10201 taccgctttc ctgcaaacgc gccatttcag taaagatatg acggtgtggg cgggtgtaaa
    10261 aatcgtctgc taccacacgc tcggctacat catcccagcg ttcgttatcc agcattaaac
    10321 cgcccaacac cgactgctcc gcttcgatcg agtgcggagg cactttcagc ccggcaactt
    10381 gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa gggtttattt cctgccatag
    10441 tgaatggagt taccgaaata aagaatgggt cgaaacttta ccatatgaag cagaccctga
    10501 cgatacgttc tggaggacac atggcaacac gaattgaatt tcacaagcac ggtggcccgg
    10561 aagtacttca agccgtagag ttcactcctg ccgatccggc ggagaatgaa atccaggtcg
    10621 aaaataaagc catcggcatc aattttatcg acacgtatat ccgcagcggc ctttacccgc
    10681 cgccatcgct acccagcgga ttaggtaccg aagcggcagg catcgtgagt aaagtcggca
    10741 gtggtgtaaa gcatattaag gctggcgatc atgtagtcta tgcgcagtcg gcgttaggcg
    10801 cttacagctc tgtgcataac attaatgcgg ataaagcggc gattctgcct gcggcaattt
    10861 cttttgagca agctgcggca tccttcctga aaggcttaac ggtttattat ctgctgcgca
    10921 aaacctatga aattaaaccc gatgaacagt tcctgttcca cgcagcggct ggcggcattg
    10981 gcttaattgc ctgccagtgg gcaaaagccc tgggcgcgaa actcatcggc accgtaggaa
    11041 ccgcgcaaaa agcgcagagc gcgctaaaag ccggcgcgtg gcaggttatt aactatcgtg
    11101 aagagaatct ggtcgagcga ttgaaagaga tcaccggcgg taagaaagtg cgcgtagtgt
    11161 acgattccgt gggcagagac acctgggaac ggtcgctgga ttgcctgcaa cgccgcggct
    11221 taatggtcag ttttggcaac tcatcaggtg cggttaccgg tgtgaactta ggcattctca
    11281 atcaaaaagg ctcgttgtat gtgacacgcc cttccctgca aggctatatc accacgcggg
    11341 aggaattaac cgaggccagt aatgaactgt tctctttgat tgccagcggt gtgattaagg
    11401 tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg catgagattc
    11461 tggaaagccg ggcgacgcaa ggttccagcc tgctgattcc ataaaagaaa tagggcttcc
    11521 acctgggaag ccctttcttt ttatagttcg gctgtatgta gggtacagca cgatgaatct
    11581 gttagaggcg caatagtgac agatttgatt atcaattcct attttgttct aaggataaaa
    11641 ccttaggttg tgatcatccg cacaatccct tagtaacgcc agcggtcata acgctgatat
    11701 ttcggcattt ttggtgcttt aatcgcctta ataacccaca ccaccgcaat cgccagtagt
    11761 aaccacggca gcaacttaat catcaatgcc agcataccgc cgaggaacat aatggccgtc
    11821 gccacaacca gcgcggcgat aatgcccagc aacgaaacgc cggtgaccat cagcatgaca
    11881 aaaaagccaa tcacaaaaag tagttccagc atgatgctct cccaaatatg aaatctcttg
    11941 ctggcattac aagaatcatg ccaaaaataa tctattgatt taacagcaaa acgccccgcg
    12001 acggtgcgca gggcgtggtg aatttgacta ctttttggtg aaaagttaac gcttatccgc
    12061 caccagtttg agcgcgtgtt ccagcacgtt aatgtcagca cccgctttat gggcattttc
    12121 acttaaataa cgccgccact gccgcgcgcc aggaataccc tggaacaaac ccagcatatg
    12181 ccggataata tggccgagat acgtcccctg gctgagttca tgctcaatgt acggatacat
    12241 ggcacgcact actgccaccg gatcggcatc ggtatccgag gaaccaaaga tctcccggtc
    12301 taccgccgcc agaatacccg gattctgata cgcctcgcgc ccgaccatca cgccatccat
    12361 atgttgcaaa tgcgctttag cttcttccag cgacttgata ccaccgttaa tcgacattgt
    12421 cagatgcggg aagtcacgct tcagttgata cacacgcgga taatcgagcg gcgggatttc
    12481 acggttttct ttcgggctta accccgaaag ccaggcttta cgtgcgtgga tgatgaacat
    12541 ctcacactcg cctttgccgg aaacggtatt gatgaaatcg cagagaaatt catagctgtc
    12601 ctggtcgtcg atgccaatac gcgttttcac cgtcaccgga atcgacacca catcgcgcat
    12661 cgctttcacg cagtcggcaa ccagctgcgc attacccatc agacacgcac caaacatgcc
    12721 gttctgcacc cggtcagacg ggcagccgac attcaggttg atctcatcat atccgcgcgc
    12781 ttctgccagc tttgcacact gcgccagcgc cgccggatcg ctaccgccca gttgcaacgc
    12841 taccggatgt tcttcttcac tgtacgccag ataatcacct ttaccgtgaa ttatcgcccc
    12901 tgtggtcacc atttcggtat aaagcaacgt attgcgggaa agcagacgca agaaatagcg
    12961 gcaatgtctg tccgtccagt cgagcattgg tgcaacacta aaccgaccac tccagtgaac
    13021 gtcagttttt tcaggcattg cgctggtttg gttgattttt tgcatttcag aattaccgtg
    13081 cattttcaaa tgtagagatt attttatcga tatatcatgg ggttatgtta ttcagcttca
    13141 ctgttcagga ggctcaatag cggggtacta taccataaca acaggaagcg cctgtctcat
    13201 tgcaaaagaa aattgagatc ctctcaaggc atgaagctct cacgaagtga tggaaataat
    13261 cttattagcc gttaactttg ttaaggccaa tgataaacaa tccaggttcg acgataaata
    13321 aaaaatcaca cattaaactc tggtgatata tctccctgct aatagcattg atagagaaaa
    13381 aagaacccaa taaatattgg gtccttttat ataatgcctt ccatactatc gaagaacttc
    13441 acatattatt tctccgattt aaccccgaac aagtcataaa ttaatttaga agtgtctgta
    13501 agtattttaa tctcttcctt tgaggttggg tcaaacgact tcgcgaagct aattaattgt
    13561 ggtgcagcat ctcgcatttt gcttaaaata tcaggatcga gcgttccttc attcaccaga
    13621 tgtgacatct tatccagata gccatcaaaa ttcatttctc ccccatctgc cagacgcttc
    13681 atctctccca ggtacttctt catatcacgt tgatataatt tttcaaactg agctttcagg
    13741 tagctctcat tattttcatt aatatacact gtttctgata aatctgcgta ggcactagaa
    13801 tatgaaagtg aaattaacag cgaagcaaat aattttaagc tgttgtcatc atgttcctgg
    13861 caggcattaa caaacgttaa aaaaccagaa cctattgctt gaaaatgtat attcgctaaa
    13921 ggttcattat ccttagattc ctcataaaat agctgaaccg tggagggaag gccattctta
    13981 gtttctacat caaaagtaca ttgttcaatg ggcattgcat ctttattatc cccaggatta
    14041 catatactat taatcgcgaa atgaaaaata gcccggtcaa caactgatgc aataaagtca
    14101 cgatcgttta aatcctcatc agtgcaatcg tcagtatact tattgaccca cattttgaga
    14161 tcatttatgt gctcaattaa aaacacattt ttttacgtgc atgccataaa ttatgcgtga
    14221 ttcgttgaat aaacaaatct atttttgcaa taattatatc ccgatgacgc agcgctgcat
    14281 aatcaataga tttaccatat atattattta atttaccttt gattgaacct ctattatcca
    14341 atttatcttt ggttgcatct ttattttcga attgatttga attgagtgtt ttattaaagt
    14401 atccagttgc accgggtatt atcttcaaca tgattcatct accttttttg tcgtaccgtg
    14461 tcctccttat aattcttttg ccatctggtc actatcaaaa atcgctcata atttctgaac
    14521 tacaggtatg cgtttaaacc cagttaaata aatagcgagt taacttgaag caatttattt
    14581 aaaaatgaat tattaattat tatcgtgttg ataatggtac gataaggcaa cacaacacct
    14641 tacctacaaa acagaaaatc aacattacgg caacaataag ggttctcggt gtttttcctg
    14701 gaacatggta aagtaaggac attcttaacc cccactttga ggtgcccgat ggaaaagacc
    14761 acaacgcagg agttattagc gcaggctgaa aaaatctgcg cgcagcgtaa tgtgcgcctg
    14821 accccacagc gcctggaagt gttgcgcctg atgagtctgc aggatggcgc tatcagcgct
    14881 tatgatctgc ttgatttact gcgcgaagct gaaccgcaag ccaagccgcc aacggtttat
    14941 cgcgcgctgg attttctgct tgagcaaggt tttgtgcata aggtggaatc caccaacagt
    15001 tatgtgctct gtcatctgtt cgaccagccc acgcatacgt cagccatgtt tatttgcgat
    15061 cgctgcggcg cggtgaaaga agagtgtgca gaaggcgtgg aagacattat gcatacgctg
    15121 gcggcaaaaa tggggtttgc cctgcggcat aatgtgattg aagcacatgg gctctgtgcg
    15181 gcatgcgtgg aagtggaagc gtgtcgtcat cctgaacagt gccagcatga tcactctgtg
    15241 caggtgaaaa agaaaccgcg ttaagagggc gtacatcctt gtacacgtcg ggcaggaggg
    15301 attaattacc agcgatattc attgcgggtt tcccaatcca cgacctcttt ttctgcctga
    15361 tctttctgat aaccataacg ttcctggatt ttaccgacca gttgatcacg tttaccttca
    15421 atgatcgtca tatcatcatc ggtcagtttg ccccattgct ctttcacttt acctttaaac
    15481 tgtttccagt taccgccggc ttcatcttta ttcataatca agacctcatc gttaggttgt
    15541 gaatgagagt acgttcactt ttcttctgaa cgtgagatta agtgtagtca gcaatttggc
    15601 ttaggataat ttattcagaa tttttaaccg tcacgttgcg gcaaaccagg taccgttgcg
    15661 ccagtgacgc cgccagatag ccgccagaga aagcccgcgc aacgccagaa agacggttaa
    15721 tgccagccac aaaccatgat tacccagcca cggcagcgta aggagcgtca gcgcaaaacc
    15781 tgcggcggcc accgccatac tgttacgcat ttcggcggca cgcgttgcgc ctataaacat
    15841 gccgtccagc agataacacc agacgccaac caacggcaaa atcacctgcc agataagata
    15901 gcggtcagcc agctgctgaa tctgggttaa cgacgtcagc aacgcaatga tgtgttcccc
    15961 tgccagcaaa taaaccaccg aaaatagtaa cgctacaatc cccgactggc ggcacgctgc
    16021 ccgccataca tccagcaact ggctaccgtc gcgcgcaccg tatgcctgac cggagtgcgc
    16081 ttcaaccgcg taggcaaaac catcaagcgc ataggcggta aaggtgagta gcgtcatcag
    16141 aaccgcgtta acagcgataa tgtcactccc cagtcgcgca ccaagtacgg tgatcgcgcc
    16201 gaaacagagt tgcaacaaca gcgaacgcag catgatatcg cggttaagcg ccagcaagcg
    16261 acggaagttt cctcgccagg cagttttcag catttcgccg gaaattccgc gtagtttgag
    16321 gattttacgc accattagca gaccaatcag caatgttgca tattccgcaa taaccgtcgc
    16381 cagcgccgcg ccctgcacgt tcatatgcag ccccatcacc agccagacat ccagcacaat
    16441 gttgaggata ttgccgacca ctaacaaaat tactggcgca cgggcatatt gcacgccgag
    16501 taaccaaccg agtaatacca gattcgccag cgacgccgga gcgcttaacc agcggatttc
    16561 aagaaagcgc cgcgcctgtt ccaggaccgc ttcactaccg ccaacaatat gcagcgccag
    16621 atcgataatc ggcgtacgca acagcgcaat taacgccccg gcccccaacg ccaacagcaa
    16681 cggttgcacc agcgcacggg ctaatgcctg aggatttttg gcaccataag cctgcgcagt
    16741 cagcccggtg gtgctcatgc gtaaaaacag caacagcata aagagaaagc tggtcgccgt
    16801 tgcaccaacc gccacgccgc ccaaataaac cggactatca agatgaccaa ttaccgccgt
    16861 atcgaccagt cccagcaacg gaacggtgat attggagaaa atcatgggta aggcgagatg
    16921 ccagagtgct ttatcagatg aagtgaggaa tgccatgcag acaagcctga tgaagagaga
    16981 tgaaaaacaa accgcgacgc caagcggcat cgcggtctca gagatatgtt acagccagtc
    17041 gccgttgcga ataaccccaa ccgccagccc ttcaatggtg aagctctgct gacgaaggtc
    17101 aacgacaatt ggtttaaact cgctattttc tggcaacagt tcgactttat tgccctgttt
    17161 tttcaggcgc ttaacggtaa cttcgtcatc aatacgtgcg acaacgacct gaccgttacg
    17221 tacatcctga gttttatgca ctgccagcaa gtcgccatcc ataatgccga tatctttcat
    17281 cgacatcccg ctgacgcgca gcaggaaatc agcattcggc ttgaacaagg aaggatcgac
    17341 ctgataatga ccttcaatat gctgttgcgc cagaagcggt tcaccggcag ccacacgacc
    17401 taccagcggc aacccttctt cctcttcctg caacagacga atcccgcgtg atgcgccgga
    17461 aacaatttca ataacgcctt tgcgtgccag cgccttcaga tgttcttcag ccgcgtttgg
    17521 ggaacggaac cccaaacgct gcgcgatttc cgcacgcgtc ggcggcatac ctgtctggct
    17581 gatgtgatca cggatgagat caaacacctc ttgttgcctg gccgttaacg ctttcattcc
    17641 gccccctggg tgtatataca gttatactgt gagtatatac agcaaaaggc gattttggaa
    17701 ccataaactg cacaataaac cagagattta tcgaaatctg gaagggttat ccaaaatgcg
    17761 accataacag gatgcaccag gtaatcacgg cgacgataat ggcaatcagc accgcggcgg
    17821 atcccatatc ttttgcgcgt ccggaaagct catggtattc agagccaatt cggtcaacca
    17881 ctgcttcgat ggcgctattg aggatttcca caatcatcac cagcatcacg gagctgataa
    17941 gcagcacgcg ggtaattgcg tccacatcca gccagcaggc gatgaccacc gccaacaata
    18001 ccgctacgcc ttcctgacgg aatgccgctt cgttgatcca tgcagcgcgt aaacctttcc
    18061 aggaatagcc agcagctttg ataattcggg tgaatccagt tgtattattg gccattgaaa
    18121 gaaccttttt acattatgag cgtcaatatc agtgtaccgt taaatccgat gattcgggtt
    18181 tgcgtaacgc cgcgcgaaac atgagcggat accacagaat ttcccatgac tttctgctat
    18241 ccttgccgcg catttgcatt attaaccaga ggctttacat cgtttatgtc cggctggcca
    18301 cgaatttact acaaattact gaatttacca ttaagcatcc tggtaaaaag caagtctatt
    18361 ccggcagatc ctgccccgga actggggctg gatacctctc gtccaattat gtacgtttta
    18421 ccgtacaact cgaaagcaga tttgctgacg ttgcgcgccc agtgtctggc acatgatttg
    18481 cctgacccgt tagagccgct ggaaatcgac ggcacgctac tgccgcgcta tgtgttcatt
    18541 cacggcgggc cgcgtgtatt cacctattac acgccgaaag aagagtctat taagcttttc
    18601 cacgactatc tcgatttgca ccgtagcaac ccaaatctgg atgtgcagat ggtgccagta
    18661 tcggtgatgt ttggtcgcgc gccggggcgt gaaaaaggcg aagtgaaccc gccgctgcgt
    18721 atgcttaacg gcgtacagaa atttttcgct gtactgtggc tcggtcgcga cagttttgtg
    18781 cgtttctcgc cgtcagtgtc gctgcgccgt atggcggatg aacacggcac ggataaaact
    18841 atcgctcaga aactggcgcg cgtggcgcgt atgcactttg cccgtcaacg tctggctgcc
    18901 gtaggcccac gtctgcctgc tcgtcaggat ctgtttaata agctgctcgc ctcccgcgcc
    18961 attgccaaag cggtagaaga tgaagcgcgc agcaaaaaaa tctcccatga aaaagcgcag
    19021 cagaacgcga ttgcgctgat ggaagagatt gcggcgaatt tctcttacga gatgatccgc
    19081 ctgactgacc gcattctggg cttcacctgg aaccgatttt atcagggcat caacgtccat
    19141 aacgccgagc gcgttcgcca gctggcccac gacggtcatg agctggtgta tgtgccttgc
    19201 caccgcagtc acatggacta cctgctactt tcttacgtgc tgtaccacca ggggctggtg
    19261 ccgccgcata tcgccgccgg gatcaacctg aacttctggc cggccgggcc gattttccgc
    19321 cgtctggggg cgttctttat tcgccgtact tttaaaggca ataaacttta ttccaccgtt
    19381 ttccgtgagt atctcggcga actgttcagc cgtggttatt ccgtcgagta cttcgtggaa
    19441 ggcggtcgtt cccgcacggg gcgtttgctg gatccgaaaa ccggtacgct gtcgatgacc
    19501 attcaggcga tgctgcgtgg cggcacgcgt ccgattacgc tgattccgat ctatatcggt
    19561 tatgagcacg tcatggaagt gggtacttac gccaaagaac tgcgcggcgc gacgaaagag
    19621 aaagagagcc tgccgcagat gctgcgcggt ttaagcaagc tgcgtaatct cggtcagggt
    19681 tacgtcaact tcggtgaacc aatgccgttg atgacctacc ttaaccagca cgtaccagac
    19741 tggcgtgaat ctatcgatcc catcgaagcg gtgcgtccgg cctggttaac gccgacggtc
    19801 aataatattg ctgccgatct gatggtacgc attaacaacg caggcgcggc aaacgccatg
    19861 aacctgtgct gtactgcgct actggcatca cgtcagcgct cactcacccg cgagcagtta
    19921 accgagcaac tcaactgcta cctggatctg atgcgcaacg taccttactc cacggactct
    19981 accgttcctt cagccagcgc cagcgagctt atcgatcacg cgctgcaaat gaacaagttt
    20041 gaagtcgaga aagacactat cggcgacatc atcattctgc cacgcgagca agcggtgctg
    20101 atgacctact atcgcaacaa cattgcgcat atgttggtgc tgccttcgct gatggcggca
    20161 attgtcaccc agcatcgcca catctcccgc gacgtattga tggagcacgt caatgtgctt
    20221 tacccaatgc tgaaagcgga gctgttcctg cgctgggatc gcgacgagtt gccggacgtt
    20281 attgatgcgc tggcaaatga gatgcaacgt caggggctga ttaccctgca agatgatgag
    20341 ttgcatatca acccggcgca ttctcgcacc ctacagctgc tggccgcagg cgcgcgcgaa
    20401 acgctgcaac gttatgccat caccttctgg ttgttgagtg ccaacccgtc gatcaaccgc
    20461 ggtacgctgg agaaagagag ccgcaccgtt gcgcaacgtc tctccgtgct gcacggcatc
    20521 aacgcgccgg agttcttcga caaggcggtg ttcagttctc tggtgctgac gctgcgtgat
    20581 gaagggtata tcagcgatag cggcgatgcc gaaccagcag aaacgatgaa ggtttatcag
    20641 ttgctggcgg agttgattac atcagacgtg cgtttgacga ttgagagtgc gacgcagggc
    20701 gaagggtaat cagagagaat tgccggatgc ggcgaaaacg ccttatccgg cctaccatga
    20761 cctgcaaatt caataaattg cgattcaccg ggtaggcctg agaagcgcaa cgcatcaggc
    20821 aacccagaag aaagccggat gatcatccgg cttttttaca tcatcagaaa tgccagtaac
    20881 tcattgccag tcctaaaaac agtaccagac caacatagtt attattcata aatgctttaa
    20941 agcaggcttc acgctcgcgg ttggcaatca gtttttgttg ataaacaaac agcgcgccag
    21001 ccaccacaat tgaccagtaa tatccccagc ctaagccatt taactcaccg atgatcgcca
    21061 tcagtgccag tacgccaatc tgcaaaatac caataatcaa tttatcgtat tggccgaaca
    21121 ggattgccgt ggatttaatg ccaatcttca catcatcatc gcggtcaacc atcgcatact
    21181 gcgtgtcgta agccaccgcc cagagaatat tggcgaggaa cattaaccag caactcaatg
    21241 gcaccgactc actcacagcg gcaaaagcca ttggaatcga ccagccaaac gccgcgccca
    21301 gcaccacttg cgggagatgg gtataccgct tcataaacgg gtacacccac gccaatgcca
    21361 gagcggcaat cgacaacaga atggtcatcg tattcagcgt cagaaccagt aaaaacgaaa
    21421 tcagtaccag cactacaaac agcgcgcgcg cctctttctc tgttaccgcg ccgctgggaa
    21481 gtggtcggtt cgccgtgcgc ttaacatgac catcaaactt gcgatcagca taatcattca
    21541 ccacgcaacc agcagcgcgc atcagccaga cacccgcgac aaacaccgcc atgatccaga
    21601 gctgaggaac gcccggtgtc gccacccaca acgcccataa tgttggccag agcagcagta
    21661 acgcgccaat tggcttatcc gtacgcatta agcgatgaaa cgccagcagc ttattctgcg
    21721 tcagactcca ctccatattt ttttcctctt agtacaacgg tgacgccggt aaaaacagtt
    21781 ctgttagcaa cagcggttta ccgcttaatc gcaggcggga acgtcgcccc cacagtccgg
    21841 catcacggcc tatctcgata aaatcccggg ttagtgtcga tgatgtgaac agatagcgtc
    21901 ctaacggcgt tttacccaat ttttgtaacg ctagttccgg cccgcttaat gttgatacag
    21961 gaacgacggt acgaccggca agccacggtt caccatcggc acataacaaa atttcacgta
    22021 accagtaacg agactctttc ggcagcagcg gcagttcttc ggggatttca ttctgctcga
    22081 caaacccctc gcggatcatc gtcacactca ccgtttttcc ctgctgttca aaacgttttg
    22141 tcatggaatc ctccagcaac agccagtcga gcagttgcgg atccagggca gggatctctt
    22201 taaaatagcg cagcgcacgc agttgcgtta acgcggggtg tgacatgccg aactctccgt
    22261 tacataacgt aaaggcattg tatcgcagaa aagtgtttac gggagatgta aagcaaatta
    22321 agtgatctaa tgcgcaacaa aaacgcaaca atcgcatgaa gtggtaaaac gctgttgaac
    22381 cttacgcttt gccgatgccc gtgctggcga gggcaaccat aggggcatca ccgcccatga
    22441 tgctaggaac ggtgatgttt tcctttactt aaataccttt attctttata cgagtagctc
    22501 gccaactcgc agtaaaaatt gggaaaagcg catatttacc atgagatttg gagctgaaaa
    22561 gtgtttcctg agtaagatta ataggcaaca tcttatgtct ggatttatag atacacgtgg
    22621 aattccaatt ggtatctttt gtaggattca acatatcatg taagcgctgg gagcttacca
    22681 ccataacagg cccatcatcg acagaggaga acaggataaa tatattatcg tttcgtgtta
    22741 atttattagt gttaaaggtt cttaactttt cttcagtttc tataaatata ggtgaaacat
    22801 catcactacg aatccactct ttataaagat accaggcatt tgctttgatc atatcatccg
    22861 ttgatcgtaa ggcctgatcg ataagttgga taaaaaaatt gttattttta tcaaccagcc
    22921 ctttattcat attaaatctg ttaataaaat ggatcatctg ttgggtatcg aaattcacct
    22981 tactgaatcg atgttgacgc ataaaattat cgaacttgtc atcttcccca gaatgtatat
    23041 aactacgtat aattggatct tttatataag gacaatggct aaatgttttc ttcatattct
    23101 caataaagtt tttattaaca atattgagaa tttttaattg catcaccagt tttactgcca
    23161 gaacggattt caatatcatt gcattatctg gcaatgtatc aatcattttt ataatgttat
    23221 tgatataacc atcgagatct tcattcccaa atatatctga ataatcaata gttaattcca
    23281 attgttctgt aatgcaatct taaaaattcc ctgtcgacat tcgcacatga tcaaacataa
    23341 cagctgtcaa attcgattta ataaatgata tttctttggc agacagaaac tctgctctgg
    23401 tgaagtgcga agcgtaaaga taacaattat cgaagtttga atttctaatc tgacattcgc
    23461 gaaaattact atgaccaagt tgactctgcg agaagtcaac tgtatcaaga atattttggc
    23521 taaaatcaac acattcgcca ctcgttttga gaaaaatcac attgtgtaaa cgtcgacatt
    23581 taaatatgac attatcaact gttgccttag taaaattgca attctcaatt tctttacatt
    23641 cgaaagttac atgttgtaga tttgatgata aaaaagacac cttagaaata acgtcacctt
    23701 taaaatgaca atttgttaaa tcaaaatcag acaggtcgat atacccctgc gcatctttat
    23761 ccatcagaaa attacaagcg cattctattt tttcatttct gtagaatttt acacttatag
    23821 tatcattact aattgagact tcacgtaaca tatttattgc atgatttttt tcgttccgtt
    23881 gttttatatt taaaaatata tcactgatat aaataaagta atctctgttt aaacctgtca
    23941 ccccattgaa attacgtagt aaatcaagat ctactatatt atttccttgc accacatcat
    24001 gtttcatcaa agagttattg ccgccagaat ttgtgatatg acctatcata aaacctctgt
    24061 aatacagaaa taaaataacc tggaaaagta gcctgagcgt ccagacacta ccggcgaaag
    24121 gaaaagaatc tctgataatg cattgaaata atggacattc ttagtgaaga agaagtgaag
    24181 tactataaaa aatcgctcat atcccaggaa ggaagcataa aaaaaggtgc gccaggagac
    24241 gcaccagttg ttgcaaaatc agcactgtgg ggcgtaatta ccccttgcct tttacactgc
    24301 tgataaaggt ggaacgggca gatgtcgatc ccaggcgttc agcttcatca agcagtttta
    24361 acgccttatc aacatcacct ttcgcgacag cgtttttgat cgcggtatta aaataacttt
    24421 ccgtgtcgtt gagcatcggc tcgcttttct tcaccggcac cggagcaggt gcagccacag
    24481 ctggtgccgc cgtgttgcct accgtaaccg gagctggagc ggaagaacca aatagcggtc
    24541 ctaccaacac gctggagctg gagttcgttt tgactttcag tttcagtaag ccatcgctgg
    24601 tatgacgagc aaccggatcg ggaatatccg ggatcgagtt accgacgcct ttggcatagg
    24661 ctttagccgg gtcgagcagt tgggtcgtct gctggagatc tttttccgtg gtaaagacca
    24721 gaacataaag tttttgctgc cccaacgccg gtgtcaggcg cataacgcct tccagccgat
    24781 ctgcactcat cacgcctagt tcctggtagg tgaaataact gctggggaag aaggctgatg
    24841 gggtcatgtt ctgatcaaga atcagcacgt tcggcgcaaa aacgctggtt tgtttgttca
    24901 cttcgctggt cagcgtcagg gtcagttcgc caatgtttgc cgggacgctg tacgcagcaa
    24961 ccggaccact gatgccggga acgttcagtt gttggccgcc ggtcgccagt tgggtggtct
    25021 gggttttaga ttgatcgacc ggtgtccagg tgagttgttg cagcgcggca gatggaatgg
    25081 ctggcgcgtc gctggtgttt tgcggtacgt agttaacatc ggcaaggcta attccaggcg
    25141 cgcttgccag taaccctgct gataaacaga gggcgatgag acttttattc attttcattg
    25201 ttttcacctc aaaatctgga gctcagcggt agccaggcaa tagcgcgcta aacccgataa
    25261 tcagaggggc tttcgcccct ttcagataat gacaacctgt ttttatgccg gatgcggcgt
    25321 aaacgcctta tccggcccag gttttgctat taccaccaga tttccatctg ggcaccgaag
    25381 gtccactcgt cgctgtcgcc acgaccgaag ctgccgccgt tgaaatcagc aggaacggct
    25441 ttgccgtagt tcgggttaac cttgctatcg ccgttgtagt cgtaacccca tttctcatcc
    25501 cacttggcgt aggttgcgaa gacacgaata gccgggcgtg accagatgct gtcgccagcc
    25561 tgccattgtt gtgcgagggt aattttgtac tgattgttct tgtcgccggt gcgctgggat
    25621 tcgacgttgt cgtagccgat ttccatcacg gtgctcatga ttggcgtcca cttgtacatc
    25681 gggcgaatac caacggtcca ccacttggtg ccgttgtcgt tatcccagtt gatatcctgg
    25741 tacataccca cgtacatcat gtcccagttg tcgcccatgg agatcgcacc gtggtcgagg
    25801 atacgcagca tgtgaccgtt gttgttgata ttgtaggcaa atttttcgtt atcaaacgcg
    25861 acgccagaac cctgcgacag acctttaccc tgcgaggtca tcgagtcagt agcgtactga
    25921 acaacaaact tgttaaagcc cttcaggaca ctctgagtat gttcagcagt gaataaccag
    25981 ccgtctttcg atgcgccatc aaccagacga tagttatcac gcaggttggc acgaccgtag
    26041 tcgacaccca gttctaatgt gccgcccggg ttgatttcca tctgcgctaa acgcacatcg
    26101 aaaacgtcgt tcgcggtttc gttggtatag tcataaatat tgttgctggc aaaagaggaa
    26161 gaaccaccag cttcagagga gcgggttgct gccagagaga gtttaccgaa gccaacatcg
    26221 atgttttcca gaccggcacc aggaccagaa atatcccagt agtagaagtc gatcatatga
    26281 acgtcatgac gttggtagaa gcgcttacct gcccagatgg tggaacctgg cagccattcg
    26341 atcaggtttt ttccctgcac gtttgcttca cggaaggccg gatcggtagc ttcccagtca
    26401 ttctgttgcg cgacggaata ggccacgtta gtgtcgaaat agaagctctt atcgccctct
    26461 ttccacactt cctgacccaa ttttaattca gcataagttt cacattcgtt gccaagacgg
    26521 tatttacttt gagcaccggt agtctggaaa cactgttgtt caccgccgct acctgtccag
    26581 ccaataccgg aacgtgcata gccgtggaaa tcaacagcca ttgcctgagc agacattacg
    26641 cccgctgcga cggcaaccgc cagaggaagt ttgcgcagag taatcatcat tctatctcct
    26701 gagtcattgc ttttcttttt tcacatcacc tgtgacaggc tttgtgtgtt ttgtggggtg
    26761 cttaaacgcc cggctcctta tgcagtcgac gacatgcagt gccatcctca cggaacagat
    26821 ggcaacgctc tggcggcagg ccgatagcga atgtggcacc ttcttctacc aacaccacgt
    26881 cgttctggcg gtacaccagg ttttgacgaa tggaagggat ctggatatag atttgggttt
    26941 cgttgccgag ttgctcgacg acctgaactt caccctcaag gatgacgtca gcgatatcac
    27001 tcggcagtag atgttccggg cgaataccca gcgacatatt ggctccaacc tggacatcac
    27061 ggctttcaac aggcagccag acttgttgac gatttggcat cggcagctcc acctgcactt
    27121 gatcgattgc ggtggcggtc acttttaccg gcaggaagtt catctttggc gaaccgataa
    27181 atccggcgac aaaacggtct gccggatagt ggtacagttc cagcggtttc ccaacctgtg
    27241 ccacgcgacc ggcgtccagc accacgattt tgtcggccag cgtcatcgct tcgacctgat
    27301 cgtgggtgac gtaaatcatt gtgcggccca ggcgtttatg cagacgggag atttcgatac
    27361 gcatttgcac acgcagtgca gcatcgaggt tggagagcgg ttcatcgagc aaaaatacgc
    27421 ttggctcggc caccagcgta cggccaatcg ccacgcgctg acgctgacca ccggagagcg
    27481 ctttcggttt gcgatccagc aaatgcgcca gttgcagcac ttccgccacc tggttgacgc
    27541 gttggttaat cacctctttt tttgcgccag ccagtttcag gccaaatgac atgttttctg
    27601 ctactgacag gtggggatag agcgcgtaag actgaaacac cataccaacg ccgcgttctg
    27661 ctggcggagt gtcattcatc cgtttctcac cgatgaacag gtcgccgctg gtgatcgtct
    27721 caagcccggc aatcatgcgc agtaaagtcg atttaccgca gccagacggt ccgacaaaca
    27781 ccacgaattc accttcatgg atatcgagat tgatatcttt cgataccacg acctcgcccc
    27841 aggctttcgt tacattttgc agctgtacgc tcgccatgcc cttctccctt tgtaacaacc
    27901 tgtcatcgac agcaacattc atgatgggct gactatgcgt catcaggaga tggcttaaat
    27961 cctccacccc ctggcttttt tatgggggag gaggcgggag gatgagaacg cggcttctgt
    28021 gaactaaacc gaggtcatgt aaggaatttc gtgatgttgc ttgcaaaaat cgtggcgatt
    28081 ttatgtgcgc atccccacat taccgccaat tctgtaacag agatcacaca aagcaacggt
    28141 ggggcgtagg ggcaaggagg atggaaagag gttgccgtat aaagaaacta gagtccgttt
    28201 aggtgttttc acgagcactt caccaacaag gaccatagat tatgaaaata aaaacaggtg
    28261 cacgcatcct cgcattatcc gcattaacga cgatgatgtt ttccgcctcg gctctcgcca
    28321 aaatcgaaga aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg
    28381 ctgaagtcgg taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg
    28441 ataaactgga agagaaattc cctcaggttg cggcaactgg cgatggccct gacattatct
    28501 tctgggcgca cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc
    28561 cggacaaagc gttccaggac aagctgtatc cgtttacatg ggatgccgta cgttacaacg
    28621 gcaagctgat tgcttaccca atcgctgttg aagcgttatc gctgatttat aacaaagacc
    28681 tgctgccgaa cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag
    28741 cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga
    28801 ttgctgctga cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg
    28861 tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa
    28921 acaaacacat gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg
    28981 aaacagcgat gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga
    29041 attatggtgt aacggtactg ccgaccttca agggtcaacc atctaaaccg ttcgttggcg
    29101 tgctgagcac cggtattaac gccgccagtc cgaacaaaga gctggcgaaa gagttcctcg
    29161 aaaactatct gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg
    29221 ccgtagcgct gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacca
    29281 tggaaaacgc ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt
    29341 atgccgtacg tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgaagccc
    29401 tgaaagacgc gcagactcgt atcaccaagt aatgctgtga aatgccggat gcggcgtgaa
    29461 cgccttgtcc ggcctacaaa actgaaacgc atgtaggcct gataagacgc atcagtgtcg
    29521 catcaggcag ttgttgtcgg ataaggcgtg aaagccttat ccgtcctgga atgaggaaga
    29581 accccatgga tgtcattaaa aagaaacatt ggtggcaaag cgacgcgctg aaatggtcag
    29641 tgctaggtct gctcggcctg ctggtgggtt accttgttgt tttaatgtac gcacaagggg
    29701 aatacctgtt cgccattacc acgctgatat tgagttcagc ggggctgtat attttcgcca
    29761 atcgtaaagc ctacgcctgg cgctatgttt acccgggaat ggctggaatg ggattattcg
    29821 tcctcttccc tctggtctgc accatcgcca ttgccttcac caactacagc agcactaacc
    29881 agctgacttt tgaacgtgcg caggaagtgt tgttagatcg ctcctggcaa gcaggcaaaa
    29941 cctataactt tggtctttac ccggcgggcg atgagtggca actggcgctc agcgacggcg
    30001 aaaccggcaa aaattacctc tccgacgcgt ttaaatttgg cggcgagcaa aaactgcaac
    30061 tgaaagaaac gaccgcccag cccgaaggcg aacgcgcgaa tctgcgcgtg attacccaga
    30121 atcgtcaggc gctgagtgac attaccgcca ttctgccgga tggcaacaaa gtgatgatga
    30181 gctccctgcg ccagttttct ggcacgcagc cgctctacac actcgacggt gacggcacgt
    30241 tgacgaataa tcagagtggc gtgaaatatc gtccgaataa ccaaattggc ttttaccagt
    30301 ccattaccgc cgacggcaac tggggggatg aaaagctaag ccccggttac accgtgacca
    30361 ccggctggaa aaactttacc cgcgtcttta ccgacgaagg cattcagaaa ccgttcctcg
    30421 ccattttcgt ctggaccgtg gtgttctcgc tgatcactgt ctttttaacg gtggcggtcg
    30481 gcatggttct ggcatgtctg gtgcagtggg aagcgttgcg cggcaaagcg gtctatcgcg
    30541 tcctgctgat tctgccctac gcggtgccat cgttcatttc aatcttgatt ttcaaaggat
    30601 tgtttaacca gagcttcggt gaaatcaaca tgatgttgag cgcgctgttt ggcgtgaagc
    30661 ccgcctggtt cagcgatccg accaccgctc gcacgatgct gattatcgtc aatacctggc
    30721 tgggttatcc gtacatgatg atcctctgca tgggcttgct gaaagcgatt ccggacgatt
    30781 tgtatgaagc ctcagcaatg gatggcgcag gtccgttcca gaacttcttt aagattacgc
    30841 tgccgctgct gattaaaccg ctgacgccgc tgatgatcgc cagcttcgcc tttaacttta
    30901 acaacttcgt gctgattcag ctgttaacca acggcggccc ggatcgtctt ggcacgacca
    30961 cgccagccgg ttataccgac ctgcttgtta actacaccta ccgtatcgct tttgaaggcg
    31021 gcgggggtca ggacttcggt ctggcggcag caattgccac gctgatcttc ctgctggtgg
    31081 gtgcgctggc gatagtgaac ctgaaagcca cgcgaatgaa gtttgattaa gggagataac
    31141 aaaaatggca atggtccaac cgaaatcgca aaaagcacgt ttgtttatta ctcacctgct
    31201 actgctactt tttatcgcgg cgattatgtt cccgctgctg atggtcgtcg ctatctcgct
    31261 gcgtcaggga aacttcgcga ccggtagcct gatcccggag caaatctcct gggatcactg
    31321 gaaactggcg ttaggtttta gcgttgaaca ggctgatggt cgcattacgc caccgccatt
    31381 cccggtactg ctgtggctgt ggaactcggt aaaggtcgct gggatttccg cgattggcat
    31441 tgtggcgctc tccaccacct gcgcctacgc tttcgcccgt atgcgctttc caggcaaagc
    31501 gacgctgctg aaaggaatgc tgattttcca gatgttcccg gcagtacttt cactggtcgc
    31561 gttgtatgcg ttgtttgatc gtctgggtga gtacattcca ttcattggcc tgaatactca
    31621 cggcggcgta attttcgcgt atctgggtgg gattgcgctg catgtctgga ccatcaaagg
    31681 ctatttcgaa accatcgaca gttcgctgga agaagcggct gcgctggatg gtgcgacacc
    31741 gtggcaggcc ttccgccttg tcctgttgcc gctgtcagta ccgattctgg cggtggtatt
    31801 catcctgtcg tttatcgctg ccattactga agttccggtc gcgtcgctgt tactgcgtga
    31861 cgtaaacagc tacaccctgg ccgtggggat gcagcaatac ctcaacccgc aaaactacct
    31921 gtggggtgac tttgccgccg ctgccgtgat gtctgcatta ccgatcacca tcgtcttctt
    31981 gctggctcaa cgctggctgg tcaacggcct gacggcaggt ggtgtgaaag gttaaagatg
    32041 ttgttctgcc aatgttatgc cgctgcaccc tcaacttacg ttatcccaac ttgtgactat
    32101 tattcggcgc tccacggagc gccttttttt ctttcgtctg caatctgaat cgttcgccgg
    32161 ttaatattcc catcatagaa cttattattt ttacgttatt tgttttccca cttacgataa
    32221 ttctctttcg tgctctgagt cacggcaata gtattgtttc atcaattttt gataattatc
    32281 acaattaaga tcacagaaaa gacattacgt aaacgcattg taaaaaatga taattgcctt
    32341 aactgcctga caattccaac atcaatgcac tgataaaaga tcagaatggt ctaaggcagg
    32401 tctgaatgaa tacccagtat aattccagtt atatattttc gattacctta gtcgctacat
    32461 taggtggttt attatttggc tacgacaccg ccgttatttc cggtactgtt gagtcactca
    32521 ataccgtctt tgttgctcca caaaacttaa gtgaatccgc tgccaactcc ctgttaggat
    32581 tttgcgtagc cagcgctctg attggttgca tcatcggcgg tgccctcggt ggttattgca
    32641 gtaaccgctt cggccgtcgt gattcactta agatttctgc tgtcctgttt tttatttctg
    32701 gtgtaggttc tgcctggcca gaacttggtt ttacctctat aaacccggac aacacagtac
    32761 ctatttatct ggcaggttat gtcccggaat ttgttattta tcgtattatt ggcggtattg
    32821 gcgttggttt agcctcaatg ctttcaccaa tgtatattgc ggaactggct ccagctcata
    32881 ttcgcggaaa actggtttca tttaaccagt ttgcgattat tttcgggcaa cttttagttt
    32941 actgcgtaaa ctattttatt gcccgttccg gtgatgccag ctggctgaat actgacggca
    33001 ggcgttatat gtttgcctcg gaatgtatcc ctgcactgct gttcttaatg ctgctgtata
    33061 ccgtgccaga aagtcctcgc tggctgatgt cacgcggcaa gcaagaacag acggaaagta
    33121 tcctgcgcaa aattatgggc aacacgcttg caactcaggc agtacaggaa attaaacact
    33181 ccctggatca tggccgcaaa accggtggtc gtctgctgat gtttggcgtg ggcgtgattg
    33241 taatcggcgt tatgctctcc atcttccagc aatttgtcgg catcaatgtg gtgctgtact
    33301 acgcgccgga agtgttcaaa acgctggggg ccagcacgga tatcgcgctg ttgcagacca
    33361 ttattgtcgg agttatcaac ctcaccttta ccgtactggc aattatgact gtggataaat
    33421 ttggtcgtaa gccactgcaa attatcggcg cactcggaat ggcaatcggt atgtttagcc
    33481 tcggtaccgc gttttacact caggcatcgg gtattgtggc gctactgtcg atgctgttct
    33541 atgttgccgc ctttgccatg tcctggggtc cggtatgctg ggtactgctg tcggaaatct
    33601 tcccgaatgc tattcgtggt aaagcgctgg caatcgcggt ggcggcccag tggctggcga
    33661 actacttcgt ctcctggacc ttcccgatga tggacaaaaa ctcctggctg gtggcccatt
    33721 tccacaacgg tttctcctac tggatttacg gttgtatggg cgttctggca gcactgttta
    33781 tgtggaaatt tgtcccggaa accaaaggta aaacccttga ggagctggaa gcgctctggg
    33841 aaccggaaac gaagaaaaca caacaaactg ctacgctgta atcttcctgt ccagcacgcc
    33901 gcgccatttc ggcgtgctgt ctttttactc ccgcttcagc cgtttcgaat tacacagcca
    33961 cagggtgatc accagtaaca ggatcgcagc cgagtagatc aacacatcca gtggcgattt
    34021 atgatcgacg atgatcaagc gcacaatggc ggtgatccca atatagacaa agtaacgtag
    34081 cgggaagtga aaaccggact gaaagtactt cacaatcagc gcgataaatt cgaaatagag
    34141 aaagtaaacc accagtcctt ctaccagctc atatttgctg gtttgttctg gcgcgaacag
    34201 cacatcagcc agatgcaccg tttctttgcc gaggaagaca accaaaatca ggccgaggca
    34261 cagcaggcca agattgagta cggtctgcaa aatggtggag ataaactcca cgcgcggacg
    34321 agagagtgac gtcatagcag tacctccttc acagtggcga gcttcctgta taacgcaaaa
    34381 atgtgacgga gatctatatt ttttgtgtat gttttgttca acctggattt ggagcgcgcc
    34441 tccaccaaag ccggatatac ctacccgtag gccggataag ccgcgcctgg cggcgcatcc
    34501 ggcaagacaa accgcaccaa agccggtaat tcaaaaatca cccattcagg tgattcccag
    34561 ccaccagcaa tgcgcgaaaa atcgacaatg ttcctctgga acaaatgaaa cgtaacctga
    34621 taaaggtggt aaaaatgaag ccttattttg ctgctttgat gttatcagtc tctgttcttc
    34681 ctgcttatgc tggtccgctc ggtactgctg ataaagccga cctaccgcaa agcaatgtct
    34741 ccagcccaat gatggcccag tcgctcaggc aaccagacct gcaaccgata tcgacggaca
    34801 ggaagacaga atgttttcgc ctttacacac cagatcgtaa accgggggtg aactgcgttc
    34861 ctgatggctc aacaggtcat taagaatgag ggtgccagtg agtgcgaaaa ctggcacctt
    34921 tcccatgact tagcggaaat ttacgctacg gtcgctggtc atgtcataca actggaactt
    34981 acgcccaagt tgctgaccac cgtcacgcgt cagcggcgtc cagccaatcg ccgcacggct
    35041 gcgtgtcggg ccagacgaga agagatccaa cggtaccgag acatacacgc ctttggtgaa
    35101 gtccccttcg ccgtactcct ctttcgaaac gttggtgatc gtggcatagc cacccaccac
    35161 cacgccgctg tcaaagcgtt tagcgatctc cagcgtgccg cctttatccc ctgccagata
    35221 ctgcccgacg ctggctttca ccaacacgtc ctgggcgaaa gatggcgtcc agtaggcggt
    35281 cagatgtccg gttttcacgc tgtagtcggt gaatttcatc atatcttttg cactacgcca
    35341 gtcgcgctgt ttaacgtagt tggcatccag accaaacgcc cagttactgt ccagcggacg
    35401 atacaacact tcggcccccg caccgccaaa catggtttcg agataacctc catagacctg
    35461 accgtagaag ccgttgccca gatgctggaa gtagttggct tgcaggttat tcacatagac
    35521 atcgttctgc acatactcgc gcacatgggt acgaacgcgc ggtaagtgcg agtcctgcgg
    35581 tggattggtg tagttaaact tgtcgtagtt gttggcaaga tttgcaaaca ggctgccggt
    35641 ggtcagtaga tggtccgtca gccacaaatc tgccgttccc atcacgccca gctgatacat
    35701 gtaaaagttt tccgggccac cgaccgactg gttcagcacc ggatcgatat ggaaatcaaa
    35761 gcgtgatttg tcgatatacc agccctgctc ggtggacttc ggaaccactg gctcgacgcg
    35821 tttgtgcgcc agcgtcgttt cgtggcccaa cggctctccg gcgagatgat ttttcaggct
    35881 ggcgacatcg gtttccgtcg tcacttgcgg catgttaagg cgattttccg taatgcggat
    35941 cgttttgatc ccatccggca gatcgttcat cacgatccga ttagcacgga tgatcccttc
    36001 gcgcgaatca cgatatttca cctgctcgcc ggtaacatac agcgtatcgc ctttcgcctg
    36061 gatctgcgga tcggcaagtc cggcgttgta tttcagcaac gttaactgat tcgccaccac
    36121 cgaatgctgc aaaatggcat cctgcggctg cggttgatat tgcgggcggg cgttatcgat
    36181 gtaggacggg cgcagatcgt taaagttggt gcgcagcgtg acgccaaaca taaaggtgtt
    36241 gccacgttca tagctaaggt taacgtcggc ccaatcggta acgcgataaa tcgccccgac
    36301 gttaaactta cttttttgct ccagcttccc ggcaaaatcc tgctgataat tattaccttc
    36361 atactccagt ttcagacgca gcggttgcca gggcgtctgg tattccacgc cgccaaacag
    36421 tgaggcagga ccgtggaaca tctggctacc gtcgatagat cccgcctgtt tgtagctgtt
    36481 atcgcgatag caatatttat cactggctga acagagcgga ttttttacat taccgctggt
    36541 gcccagatat ccccagccca ggccgagcgt aaaatcgaac ggcccccagg ctttgctggc
    36601 aacaagatat tccgcatcaa acagccccgt accgccgata tcccgcgcac caaccgccac
    36661 ttgcggcagc cagtaactct cttcccacaa acgcagtttg agatcgaagg ctttatcttt
    36721 atacgtttga tcgccagaga acgcttcgac gctgctgtac tgccgggtgc gcacgtcggt
    36781 gtagcgcagc gttgtttcca gccacgggaa gagttgcact gaagctgagt aataacggta
    36841 ctgatcgtta tcgcgatagt tcagactcaa ctccccttcc cgtgccatac gcgcggtggg
    36901 agtttgtaat aatcctacgc caccgaaatc cgactgcgac ggaccaatgg gcgcaggata
    36961 tgtttcgccg tagcaagctg tgctaatgcc cagcgccagt aagctaagca gatgtctttt
    37021 tttcattatt ggggtatccg ctgcgtcaga gtctgaagaa tgtcggcgtt aagggcatca
    37081 ggcgtctcac tccagacgga atctgcgagg ccaacataaa taatgctgcc gggcatcggc
    37141 tctacgtgac gcttgttcca gtaagccacc ggcgcttttt gcgtgcgtcc gtccgggtaa
    37201 acgacccacg cgtagctgcg atccgcaccg ctgagcaggc tctggtcaga gagatagctc
    37261 gccacgtcgc gacctggagt gaatggctga ttgccaggat ggctgataag cccgaacaac
    37321 gtgaccgtgg acggcggtgg tccgacccac agcgtgtagt tgccctgcaa cggcgggtta
    37381 ccgcgttcgg caacgcggac gatatcggga tcaagattga ttttttgcct gcctgtcact
    37441 ttcaatgcct ggatttgctg gcgtaaggcg ttaatggcgg cggcatcgtc ggtgctggaa
    37501 tctgcgccct gttctgccag ccgtgtcagc aacgcctgct gctgacgcaa tgctgccgcc
    37561 gttgccagct cttcgctaat caccgcacca ggccaccagc tgtttgccag tcgaggttgc
    37621 cctaccagat cgattaaatg ctctgcgccc gttagcgttt tggcctcact gctgccattg
    37681 ctgaacacct tgacggttcc tgccgcgaag accgatgacg ctcccacact caaaagcaac
    37741 gcgacaatag tttgtttaat cattgttttg ccgccttgat gagagtggtt ttcaccggga
    37801 aataatccgc gccgagatac tgttccgact ggcgaatttg cccttcgctg tcgatccagt
    37861 agcggttatg ccagcgcgcc tggtcggtgg agacttcttc gtccagcacg cgaaccaggg
    37921 tttcatcgct gccgaccttg acggtatcgg tgccatccca tttgaagact gagcgtgcgg
    37981 tggcgtagcg tacctgctgg tactcggtcc agcccatcgt gcgcgtccat gttgcaccat
    38041 caacaatttg tgcgggttta atcagcgggt cggcggcaag gttattcact tcaatcaggt
    38101 tgtcgccgcc aagcaaagtc ttcaccagac gaccatgttg tgtgacaaga gtggcttgat
    38161 cctgagtgac ccatttttgt tgtccgtctt cagcgaaggc cagcaccaca aacaattgcg
    38221 gcccgccatt aagctgcatg tactggctgg cgtagggcat attttgaata tcatcgtccg
    38281 tcagctgtac gcctggcgtg ccgaacagac tgtcccacag tgaattgccc agctctttag
    38341 tcgtggctga acaggcctgt aatagcaggc agataagaat gagtgcaggt cgcttcacga
    38401 ctcttctcca gaggggcaga ataaccacac cggagtgtgg ttatggtaaa tcccttcata
    38461 cttcaagcca cagatgcaat gactcaaatt attttgggga tacatacttt attactgggt
    38521 actggtggtc gtggttgtgg tggtcccggt attagaacca tcaccgccac cggttgccgc
    38581 gagcgcgaca cccaccgccg agcttacggt gctgacgctg gtcgcggagg aactccccgc
    38641 cgaaaccgac gttgctgccg actctgccgc ttcgcccacc tgtacaggtg cagcccacgc
    38701 agaagtcgcc gcaagcgcag atatggcaaa aatgccatac agaacttttt tcataacaat
    38761 ttcccttcat tgaatgaatg gagatttacc caaaaatatt tcgggcggga tcgagtatac
    38821 acaactaaag catgcgaatt acggcgcggg gaattagcga tggagtttta ggataattgg
    38881 aatgaatatc aaatataagt ttcaacttat atttaaataa caataaaaat cataagctta
    38941 aaataaaaaa caaaagtaat taatctgcgt attctccgaa ataaacaacg attaaaaatc
    39001 tattttagga ttaacctcac ggtatgattt ccgttaaatc acagacaagg ggatttatct
    39061 gataaaacaa tgccgactta ttgtcggcat tgtttttaag gttatttaac tcctcatact
    39121 atgcattaat gtcgatattg gcatcaggct ccggttgccg gatgcggcgt aaacgcctta
    39181 tccggcctac atatcggcat caggcacgca tgatgattaa ccgcgccacg ctttatagcg
    39241 gttaatcaga ccattggtcg agctatcgtg gctgctgatt tctttatcat ctttcagctc
    39301 tggcagaata cggtttgcca gttgtttacc cagctccacg ccccactggt cgaaggtgaa
    39361 gatgttcagg atcacgccct gagtaaagat tttgtgctca tacagcgcaa tcaacgcacc
    39421 caggctgaac ggagtgattt cgcgcagcag gatggagttg gtcgggcggt taccttcgaa
    39481 cactttgaac ggcaccacgt agtcaagcgt tgccggatct ttaccctgat cgcgatattc
    39541 ctgctcaacc acttcgcggg atttaccaaa cgccagcgct tcggtctggg cgaagaagtt
    39601 agacagcagt ttctggtggt gatcagagag cgggttatgg gtgatagccg gggcgatgaa
    39661 atcgcacggt accattttgg ttccctggtg gatcagctgg tagaacgcgt gctgaccgtt
    39721 agtgcctggt tcaccccaga taatcgggcc agtctggtaa tccacaacgt taccgttacg
    39781 gtcaacatac ttaccgttgg actccatatt gccctgctgg aagtacgccg cgaaacggtg
    39841 catatactgg tcatacggca gaatcgcttc agtttccgca ccaaagaaat tgttgtacca
    39901 gatgccaatc agcgccagca ggacaggcag gtttttctcg gcaggcgtgg tggagaaatg
    39961 cttgtccatt gcgtgtgcgc cagaaagcag ttcaacgaag ttatcaaagc cgatggagag
    40021 aacaatcgac aggccaatcg ctgaccacaa agagtaacgg ccgccgaccc agtcccagaa
    40081 ctcgaacatg ttggcagtat caataccaaa ctcgccaacg gctttggcat tggttgagag
    40141 cgccgcaaaa tgtttcgcaa cgtgtttttc atcacctgcc gctttcagga accagtcacg
    40201 cgcgctatgg gcgttggtca tggtttcctg agtggtgaag gttttagatg ctaccaggaa
    40261 cagcgtggtt tccgggttta cttttttcag cacttccgag atgtgagtcc catcgacgtt
    40321 agaaacaaag tgcatgttca ggtggttttt gtacggacgc agtgcttcgg tcaccatgta
    40381 cgggccgagg tcagaaccgc cgatcccgat gttcactacg tcagtgattg ctttgccggt
    40441 ataacccttc cactcaccgg agataatcgc ttccgagaag gttttcatct tctccagcac
    40501 cgcgttgact tctggcataa catctttgcc atcaaccaga atcggggtat tgctacgatt
    40561 acgcagcgct acgtgcagca cggcgcggtt ttcagtgcgg ttgatcttct cgccagagaa
    40621 catcgactta atcgcgcccg ccagatcgca ctctttcgcc agatcctgta atttcgccag
    40681 cgtctcttca gtgatgcggt ttttggagta atccaccagc atctgatcgt cgaaggttgc
    40741 ggagaactta gaaaaacgat cgccgtcttt agcaaaaaga tcggcgatcg taacgtcttt
    40801 catttcatcg aagtgtttct gtagtgcctg ccaggcagcg gtctgcgttg gattgatgtt
    40861 tttcattagc aatactcttc tgattttgag aattgtgact ttggaagatt gtagcgccag
    40921 tcacagaaaa atgtgatggt tttagtgccg ttagtgtaat gttgagtgta aacccttagc
    40981 gcagtgaagc atttattagc tgaactactg accgccagga gtggatgaaa aatccgcatg
    41041 accccgtcgt tgacaaccgc cccgctcacc ctttatttat aaatgtacta cctgcgctag
    41101 cgcaggccag aagaggcgcg ttgcccaagt aacggtgttg gaggagccag tcctgtgata
    41161 acacctgagg gggtgcatcg ccgaggtgat tgaacggctg gccacgttca tcatcggcta
    41221 caggggctga atcccctggg ttgtcaccag aagcgttcgc agtcgggcgt ttcgcaagtg
    41281 gtggagcact tctgggtgaa aatagtagcg aagtatcgct ctgcgcccac ccgtcttccg
    41341 ctcttccctt gtgccaaggc tgaaaatgga tcccctgaca cgaggtagtt atgtctgaaa
    41401 ttgttgtctc caaatttggc ggtaccagcg tagctgattt tgacgccatg aaccgcagcg
    41461 ctgatattgt gctttctgat gccaacgtgc gtttagtagt cctctcggct tctgctggta
    41521 tcactaatct gctggtcgct ttagcggaag ggctggaacc tggcgagcga ttcgaaaaac
    41581 tcgacgctat tcgcaacatc cagtttgcca ttctggaacg tctgcgttac ccgaacgtta
    41641 tccgtgaaga gattgaacgt ctgctggaga acattactgt tctggcagaa gcggcggcgc
    41701 tggcaacgtc tccggcgctg acagatgagc tggtcagcca cggcgagctg atgtcgaccc
    41761 tgctgtttgt cgaaatcctg cgcgaacgcg atgttcaggc acagtggttt gatgtacgta
    41821 aagtgatgcg taccaacgac cgatttggtc gtgcagagcc agatgtagcc gcgctggcgg
    41881 aactggccgc gctgcagctg ctcccacgcc tcaatgaagg cttagtgatc acccagggat
    41941 ttatcggtag cgaaaataaa ggtcgtacaa cgacgcttgg ccgtggaggc agcgattata
    42001 cggcagcctt gctggcggag gctttacacg catctcgtgt tgatatctgg accgacgtcc
    42061 cgggcatcta caccaccgat ccacgcgtag tttccgcagc aaaacgcatt gatgaaatcg
    42121 cgtttgccga agcggcagag atggcaactt ttggtgcaaa agtactgcat ccggcaacgt
    42181 tgctacccgc agtacgcagc gatatcccgg tctttgtcgg ctccagcaaa gacccacgcg
    42241 caggtggtac gctggtgtgc aataaaactg aaaatccgcc gctgttccgc gcgctggcgc
    42301 ttcgtcgcaa tcagactctg ctcactttgc acagcctgaa tatgctgcat tctcgcggct
    42361 tcctcgcgga agttttcggc atcctcgcgc ggcataatat ttcggtagac ttaatcacca
    42421 cgtcagaagt gagcgtggca ttaacccttg ataccaccgg ttcaacctcc actggcgata
    42481 cgttgctgac gcaatctctg ctgatggagc tttccgcact gtgccgggtg gaggtggaag
    42541 aaggtctggc gctggtcgcg ttgattggca atgacctgtc aaaagcctgc ggcgttggca
    42601 aagaggtatt cggcgtactg gaaccgttca acattcgcat gatttgttac ggcgcatcca
    42661 gccataacct gtgcttcctg gtgcccggcg aagatgccga gcaggtggtg caaaaactgc
    42721 atagtaattt gtttgagtaa atactgtatg gcctggaagc tatatttcgg gccgtattga
    42781 ttttcttgtc
//
LOCUS       DAJTSA010000027        40532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000027 DAJTSA010000000
VERSION     DAJTSA010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.027"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..955)
                     /locus_tag="OO369_001551"
     CDS             complement(<1..955)
                     /locus_tag="OO369_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR5981873.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAG"
     gene            1201..1827
                     /gene="gstB"
                     /locus_tag="OO369_001552"
     CDS             1201..1827
                     /gene="gstB"
                     /locus_tag="OO369_001552"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCR5981874.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1824..2938)
                     /gene="yliI"
                     /locus_tag="OO369_001553"
                     /pseudo
     CDS             complement(1824..2938)
                     /gene="yliI"
                     /locus_tag="OO369_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3049..3432)
                     /gene="bssR"
                     /locus_tag="OO369_001554"
     CDS             complement(3049..3432)
                     /gene="bssR"
                     /locus_tag="OO369_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR5981875.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3645..4970
                     /gene="rimO"
                     /locus_tag="OO369_001555"
     CDS             3645..4970
                     /gene="rimO"
                     /locus_tag="OO369_001555"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR5981876.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5017..6345)
                     /locus_tag="OO369_001556"
     CDS             complement(5017..6345)
                     /locus_tag="OO369_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5981877.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6353..8695)
                     /locus_tag="OO369_001557"
                     /pseudo
     CDS             complement(6353..8695)
                     /locus_tag="OO369_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8873..9784)
                     /gene="gsiD"
                     /locus_tag="OO369_001558"
     CDS             complement(8873..9784)
                     /gene="gsiD"
                     /locus_tag="OO369_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR5981878.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9787..10707)
                     /gene="gsiC"
                     /locus_tag="OO369_001559"
     CDS             complement(9787..10707)
                     /gene="gsiC"
                     /locus_tag="OO369_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR5981879.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10725..12263)
                     /gene="gsiB"
                     /locus_tag="OO369_001560"
     CDS             complement(10725..12263)
                     /gene="gsiB"
                     /locus_tag="OO369_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR5981880.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12283..14154)
                     /gene="gsiA"
                     /locus_tag="OO369_001561"
     CDS             complement(12283..14154)
                     /gene="gsiA"
                     /locus_tag="OO369_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR5981881.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14141..15106)
                     /gene="iaaA"
                     /locus_tag="OO369_001562"
     CDS             complement(14141..15106)
                     /gene="iaaA"
                     /locus_tag="OO369_001562"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5981882.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15310..16545
                     /gene="moeA"
                     /locus_tag="OO369_001563"
     CDS             15310..16545
                     /gene="moeA"
                     /locus_tag="OO369_001563"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR5981883.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            16545..17294
                     /gene="moeB"
                     /locus_tag="OO369_001564"
     CDS             16545..17294
                     /gene="moeB"
                     /locus_tag="OO369_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR5981884.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17473..18135)
                     /gene="fsa"
                     /locus_tag="OO369_001565"
     CDS             complement(17473..18135)
                     /gene="fsa"
                     /locus_tag="OO369_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR5981885.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18266..19165
                     /locus_tag="OO369_001566"
     CDS             18266..19165
                     /locus_tag="OO369_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR5981886.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19171..21603
                     /locus_tag="OO369_001567"
     CDS             19171..21603
                     /locus_tag="OO369_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR5981887.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21749..22564
                     /gene="ybiV"
                     /locus_tag="OO369_001568"
     CDS             21749..22564
                     /gene="ybiV"
                     /locus_tag="OO369_001568"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR5981888.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22716..23981
                     /locus_tag="OO369_001569"
     CDS             22716..23981
                     /locus_tag="OO369_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR5981889.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24222..25814)
                     /locus_tag="OO369_001570"
     CDS             complement(24222..25814)
                     /locus_tag="OO369_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR5981890.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26033..26953
                     /gene="ldtB"
                     /locus_tag="OO369_001571"
     CDS             26033..26953
                     /gene="ldtB"
                     /locus_tag="OO369_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5981891.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27011..28129)
                     /locus_tag="OO369_001572"
     CDS             complement(27011..28129)
                     /locus_tag="OO369_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR5981892.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28126..28593)
                     /gene="mntR"
                     /locus_tag="OO369_001573"
     CDS             complement(28126..28593)
                     /gene="mntR"
                     /locus_tag="OO369_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR5981893.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28779..28907
                     /gene="mntS"
                     /locus_tag="OO369_001574"
     CDS             28779..28907
                     /gene="mntS"
                     /locus_tag="OO369_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR5981894.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29179..30762
                     /gene="opgE"
                     /locus_tag="OO369_001575"
     CDS             29179..30762
                     /gene="opgE"
                     /locus_tag="OO369_001575"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR5981895.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30810..31325)
                     /gene="ompX"
                     /locus_tag="OO369_001576"
     CDS             complement(30810..31325)
                     /gene="ompX"
                     /locus_tag="OO369_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR5981896.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31378..31443)
                     /locus_tag="OO369_001577"
     CDS             complement(31378..31443)
                     /locus_tag="OO369_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981897.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31678..32565
                     /gene="rhtA"
                     /locus_tag="OO369_001578"
     CDS             31678..32565
                     /gene="rhtA"
                     /locus_tag="OO369_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR5981898.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32864..33367
                     /gene="dps"
                     /locus_tag="OO369_001579"
                     /gene_synonym="pexB"
     CDS             32864..33367
                     /gene="dps"
                     /locus_tag="OO369_001579"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR5981899.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33771..34517
                     /gene="glnH"
                     /locus_tag="OO369_001580"
     CDS             33771..34517
                     /gene="glnH"
                     /locus_tag="OO369_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR5981900.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            34656..35315
                     /gene="glnP"
                     /locus_tag="OO369_001581"
     CDS             34656..35315
                     /gene="glnP"
                     /locus_tag="OO369_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR5981901.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35312..36034
                     /gene="glnQ"
                     /locus_tag="OO369_001582"
     CDS             35312..36034
                     /gene="glnQ"
                     /locus_tag="OO369_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR5981902.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36295..38519
                     /gene="ybiO"
                     /locus_tag="OO369_001583"
                     /pseudo
     CDS             36295..38519
                     /gene="ybiO"
                     /locus_tag="OO369_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38516..39431)
                     /gene="rlmF"
                     /locus_tag="OO369_001584"
                     /pseudo
     CDS             complement(38516..39431)
                     /gene="rlmF"
                     /locus_tag="OO369_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
     gene            39707..39967
                     /locus_tag="OO369_001585"
     CDS             39707..39967
                     /locus_tag="OO369_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5981903.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            40232..>40532
                     /locus_tag="OO369_001586"
     CDS             40232..>40532
                     /locus_tag="OO369_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5981904.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT        10097 a        10714 c         9861 g         9860 t
ORIGIN      
        1 agcccgcttc gcctgccccg agattcactt cgatcttatc accaaaccag acgcgctgag
       61 tcacaaaggt ggcatcaggt ttaattggcg tcacagtttc aaagaagcgg aaaccccagg
      121 tcaataattt ctcagactca ttaaaacgga tacggtcagt tttcgccccc agcactacgg
      181 agattaaacg catatcaccc tgggtagccg aagcaaccag attatatccc gcacctgccg
      241 tggttcctgt cttcatgcca tcaacattca gattgctgct ccacagcaga cggttacggt
      301 taggctgacg aattttgttg aaggtgaact ctttctcttt atgaatggcg tactcttccg
      361 gcacatcgtg gatcaatgct ttacccagca atgccatatc gcgcgcggta ctgaactgcc
      421 ccggcgcatc caggccgtgc accgtctgga aggtagtgtt ggtcagcccc agttttttgg
      481 cataaccatt catcagacca ataaaggact cctggctccc ggcaacgtaa tctgccagcg
      541 caatacaggc gtcattaccg gactggataa tcacaccttt gttcaagtct gccaccgaaa
      601 cctgatcgcc cggtttgagg aacattaccg atgaaccacg cagtgccgga tttcccgtcg
      661 cccaggcatc tttaccgacc gtcaccatat cggtgagttt aatcttatcg gccttaagcg
      721 cctgcccaac cacatagctg gtcatgattt tagtcaggct cgcaggatcc agtttctcat
      781 ccgcattgcc ttctgccagc actttaccgc tggcgtaatc cattaaaatc catgcacgcg
      841 catccacgct cggcgcttca acggtttgtt cggccgcgaa tgccgttggg gcaaaaagga
      901 ataaaaatgc agaacccgct gcaagaccac gaaggagaga ggagtattgc gtcataaatg
      961 ccacccgagt atccattcaa aaaaatacgt tatatcgccg tatgataagt tcggtgatta
     1021 ataacgcaca cacaaactta aagaaaccgt aagttggtaa agtttttaaa gtttatgcaa
     1081 tatcctgccg cttcgctgct tctggtgtca tttttttgcc agctgttgct taaaagttaa
     1141 ctttacttca ccatcggagt cctgttcagt ctgtggctgc ctgtcggaaa aggggtgagt
     1201 atgattacgc tgtggggtcg gaataattca actaacgtaa aaaaagtatt gctgacgctc
     1261 gaagaactgg aactacctta tgagcaaatt ctcgcgggcc gtgagtttgg gataaatcac
     1321 gatgctgatt ttctggcgat gaaccctaac gggctggtgc cgttgttgcg tgacgacgaa
     1381 agtgatctca ttctttggga atcaaacgcc attgtccgct atctggcggc acagtacggg
     1441 caaaaacgcc tgtggatcga ctcaccggca cgtcgtgcgg aagcggaaaa atggatggac
     1501 tgggcaaacc agacgctcag taatgctcat cgcgggatcc tgatgggatt agtcagaaca
     1561 ccaccggaag agcgcgatca ggccgccatt gatgccagtt gcaaagagtg cgacgccctg
     1621 tttgccctgc tcgatgcgga actggcaaaa gtaaaatggt tctctggcga cgagtttggt
     1681 gtgggcgata tcgctatcgc accgttcatc tacaatttgt ttaacgttgg cctgacctgg
     1741 acaccgcgtc caaatctgca acgctggtat cagcaactca ctgaacgccc cgcagtgcgc
     1801 aaagtggtga tgattcccgt tagctaattg cgtgggctaa ctttaagtaa ttccccactg
     1861 gactcgtcgg tgagaacgta taaataaccg tcgggtccag tgcgaacatc acgaattcgc
     1921 tgccctctgt ccgttaaaat acggccatct tctgtcactt tgtcgccgtt gacgctcatc
     1981 acaatgacat ctttatcttt cagctcccaa taaataattt ttgctgccac tgggggaatt
     2041 tatcgctgtt atagaaggcc atgccgctca cagcgggcga atctttccag taaaaaacag
     2101 gttgctcggt cccggcgacg atctcccctt tcgcttccgg tatcttaaag cctgaatagt
     2161 tgattcccca ggttgccagc ggccagccgt agtttttgcc tttttgcggg atattaattt
     2221 catcaccacc gcgcgggcca tgttcattca gccacagtgc attactccac ggattcatcg
     2281 ccattccttg cggattacga atgccataag accagatctc ggcgcgcgca ccggattcct
     2341 ttataaaagg attatcatcc gggatttcgc cctggtcggt cagacgcacc agtttgccct
     2401 gtaatttatc cagatcctgc gccgtcgggc gctgattgtt ttcgcccaga gcaataaaaa
     2461 gataaccttt accgtcgaat accagccgcc cgccaaaatg gttgccggta gacagttttg
     2521 gcatctggcg gaagacggtg cggaagtcgg tcacttttga gagatcatca cttaagcggc
     2581 cataacccac agcagttccg gctttgccat catcgccaac ttcggaataa cttaaccaga
     2641 tgcggcgaga ctgagcaaaa tcaggcgcta aaaccacgtc cagcaggccg ccctgcccgt
     2701 gcgcccaaac gtccggaact ccggaaagcg gcgcagataa tccttttcct gcttgccagt
     2761 gacgcaactc gccgccgcgc agagtgatta acataccgtg attatcgggt aaaaaggcca
     2821 gtgcccaggg atggtcgagt ttgtcttgca gtacttcgac atttaccgtt gcaggagccg
     2881 cccagagagc ggaagaaaga caaataaggg gcacaaggaa aaaggattgt cgatgcataa
     2941 cgcctccgga ttgactctgg cttaaagcgt agtcagtgga ggagataaca aattcatttt
     3001 tacaaaaact taaacatgaa gggggagacg ctttctcccc cttagttttc aggccttctc
     3061 aagcatggcg tgcttctgca ggctctggat actcagcgtt aagctcatca gacaattttc
     3121 aagcttatcg gcgttgacgg taataacagt cgggcaatca tggtgcccac tcatcaaaca
     3181 tactgcggct gtcgctaatg cttcttcagc atgatgaaga gcactccact cttcctgatc
     3241 cagatgaaga ttcaaccgca gcgatttatc gtgcagttcg cgattcagtt taaaaaagtt
     3301 atctcgtaga tgattgcttt cgctgacgga catgtatcct tttgccttcc tcagttgcac
     3361 gtatgcagcg aggtcaacgc gtgcgtcgat caaccggttc agcagatcga ttcgctgtct
     3421 gtcaacgaac atacttcgat cctcctcttt ccagccttgt tgcaattaaa tagtatggct
     3481 attttttgag caaaatactg tccaggatca attcttcacg ccgaattaac aaaatttaca
     3541 tttccttcgc ctccgccagc gaatgagatt gcgatacaaa ttttatccag aactctgtag
     3601 aatccctgcc ctgacctttc ctgtaactga atatttttta agctatgagc aaagtaactc
     3661 cccagccgaa aatcggcttt gtttcccttg gctgtccgaa aaaccttgtc gattcagagc
     3721 gtattctcac cgaactccgc actgaaggtt atgacgtggt accgagctat gacgatgcga
     3781 acatggtgat cgtcaacacc tgcggcttta ttgacagcgc ggtacaagaa tcactggaag
     3841 ccattggtga agcgttgaat gaaaacggca aggtaattgt gaccggttgt ctgggggcaa
     3901 aagaagatca gatccgcgaa gtccacccga aagtgctgga aatcaccggg cctcatagct
     3961 atgagcaggt tctggagcac gttcatcact acgtgccaaa accgaaacac aacccattcc
     4021 tgagcctggt gccagaacaa ggtgtgaaac tgacgccgcg tcattatgcc tatctgaaaa
     4081 tttcggaagg ctgtaatcac cgctgcacct tctgcattat tccgtctatg cgcggcgacc
     4141 tgatgagccg tccgattggc gaagtgttaa gtgaagcgaa acgtctggta gatgcgggcg
     4201 ttaaagagat tctggtgatc tcgcaggata cttccgccta tggcgttgat gttaaacatc
     4261 gtactggctt ccacaacggc gagccggtaa aaaccagcat ggtcagcctg tgcgaacagt
     4321 tatcgaaact ggggatctgg acacgtctgc actacgttta cccttatccg catgtggacg
     4381 acgtcatccc actgatggca gaaggcaaaa tcctgccgta tctggacatt ccgttgcagc
     4441 acgccagccc gcgcattctc aaactgatga agcgtccggg ttctgtagat cgccaactgg
     4501 cgcgcatcaa acagtggcgc gaaatctgcc cggaactgac cctacgctca acctttattg
     4561 tcggcttccc tggcgagacg gaagaagatt tccagatgct actcgacttc ctgaaagaag
     4621 cacgtctgga tcgcgttggc tgctttaaat acagcccggt tgaaggtgca gacgccaatg
     4681 ccctgcctga ccaggttccg gaagaagtga aagaagaacg ctggaaccgt ttcatgcagc
     4741 tgcagcagca gatttccgcc gagcgcctgc aagagaaagt gggccgtgaa attctggtga
     4801 ttatcgacga agtggacgaa gaaggcgcga ttggtcgcag catggcagat gcaccggaaa
     4861 tcgacggcgc ggtttatctc aatggtgaaa ccaacgttaa gccgggtgat atcctgcgtg
     4921 tgaaagtcga gcacgccgat gagtacgatt tgtggggtag ccgggtttaa ttaattcagg
     4981 ccgggcaaaa tcatttgtcc ggcctgcgaa taatgattac tgccagctaa tctgtgccct
     5041 gccgtttttc ttactcttat acagccgctc atccgccagc attttcaagc gttcgacggt
     5101 agtacattca tttgaatgcg ctacaccgat actggtgctt aaattaatgc ttcccacctg
     5161 gaaatgaacg atttctttta gtatcgatag caactcttcc agcgtgccgc cagtataaat
     5221 caccgcaaat tcgtcacctc cgaagcggta gactttacct ttatctaccg ggatctgtga
     5281 tttaaggatt ttcaccactt tcatcaacac ttcatcgccc agcaaatgac cgaaggtgtc
     5341 gttaatgttt ttgaatttat cgacgtcaaa cagacacaaa taaccgcctt tttccggcag
     5401 cgcttttaaa tcttcatcaa aggattttct gcccattgct ttggtcaggt cgtccaggaa
     5461 cgccgagtta tacatactgt agagtttttt gacatcggca gccgaaaaga aaagcccgcc
     5521 atccagtacg cgttcaaaat ggttattaat gatggcttct ttctccacaa taccacgatt
     5581 aatcactctg cgtaccagat agaggcaact ggcacccaat atcactgcca ccaatgcggc
     5641 aaagaaaata atcaatgtac aaacccgcaa ataaacatct tttttatacg caaatacgta
     5701 caatccggtc ggcaaatagg cgaagtgctc catcgcttcc gaaacggtga agatggcgtt
     5761 tttagcatta tattcatcac cggtcaccgg tttaccgttc ttaaatagag agaacatcag
     5821 gttctcgctg tccatcacca gcgattttga cagtaaagaa aaacgctcca gaccaagcaa
     5881 aaatatcaga ctacgattgt gagttaccgg atagaggtaa tagacgaagg ttttaccgtc
     5941 aaaagagata aaccctgtat ttttgacatg tcgatacttc acaacctgca taatatctgc
     6001 acaataattt ttctcgaact cctttacctg aatacattcg tcactggaaa tcagataagg
     6061 accgacatcg tacaaataag agggggaaac cgagtcggcg taagctgtgc gcaaaccaac
     6121 caacagctgt tcctccgtgg gatttagcgt gttgataaga tcaattgcca cgctacgccg
     6181 atattcagca atatttgcca ccgcaaaagc agtatcaata acggactgtt tcaggctata
     6241 cacccgttcc tttaccattt gagtgtagat cccgcaggca actgctgaaa tcatgataaa
     6301 aatcagcaga ctgactgtaa gtacgaactt attgattctg gacatacgta aactactctt
     6361 ttactaattt tccactttta tcccaagcgg agaatggcat gggtttctgg tacagatacc
     6421 cctgagcctg aacacatcct aatttaatta attcgcgcgc aacgtcttcg ctctctacgc
     6481 cttccgcaat caccgggatc cggttatagc gtgccagatc gaaaataatg cgcacaattt
     6541 ttcccgcagt accgaaaata tcgccggtaa attgcgagtc cacctttatg ctgtctggat
     6601 taatttcata aaaacgtttc aggtttgaca aaccggtgcc aaagtcatca atcgatatcc
     6661 gtaccccttg tttacgcagt tgttcaatat tgagataaaa atgttcatta attcgctcga
     6721 tcacggtttc agtgatctca acacagaggc tgttcgcctt caggccattt tctttcatga
     6781 tggtggtaaa acgctcatga aagtgcggtt cgtttaactg ccagggcgaa acattaatat
     6841 gcagtaagaa atcatccttc agcgacgcac gttgactaaa atgtctgaat taacccattg
     6901 ccgttttaat aatctgataa cccagctcat tgataaaccc gatatcttca gcaatggtaa
     6961 tgaacttcag cggtgaaatg atcccccgct ccgcagaaac ccaacggcac aacgcctccg
     7021 cgctcagaat ggcgcgggtg ttaatatcaa cgatcggttg atagacgagg aatagttcgc
     7081 caatttccat tgcatggcga atattacgcg caatccagat attctcttct atttcgttga
     7141 gtttcgcgtc atcacagaaa ataaactcgg agaacatatg gtcacggata ctggaaatcg
     7201 ccagtttcag tttttctgcg taactccagg cgttttcccc ctgcaaaggt tcacaaatgg
     7261 cagacttatg gatgcgcaga ttgcggtagc caaagctgct gtcaaaatcg tttaccatcg
     7321 ctattttacg ggtaaatgtc tgtacatttt cccgacacag aagtaaatag agatcggcat
     7381 tgaatgtcac gatatcgcag cattgcgcat atttctcggc aattcgctga cacaatttcc
     7441 tgacgatttt attgcgttca tcaacaccaa acaccgcctc cacggcttcc agaccacaca
     7501 gtttgatgat ccccacagcc agatactgat tattgttgga ctcaataatc gccatatttt
     7561 taataaaacc gctttcctga tcgtaagaga tcagtgagag caatttattt gaccgttcaa
     7621 tttcggcatc tttcatttcc gcgatcgaat ttttgatgct gataatctct ttcgcatcgc
     7681 cttcgcgaat tttcagagcc tcgatctgat ccgttgagtc gggcaaaaac gccagcgtat
     7741 taaccagctc ggtcatttca gaaaattgcc gacggaacac acgagtggtc atgaaatata
     7801 ccaccagcaa cacgataata atgaacgcaa acggtagata aaaaagttta tctgatgcgt
     7861 tcaataaatt accgccagga taataaaaat agatttcgaa caatgcatta tttggcactg
     7921 ctgatgtctt tgcaatatac tccgtatcga cagaatcaat aatattataa ttaccttcgc
     7981 tattataaat aatcagtttt tcacgagcga gttgattctt ggcaatgatc tcatgacctt
     8041 gtttcaaaaa tagaacttca tcattagcgc tgatttgatc aagatactcg ttcagatacc
     8101 gccagtttaa atcgacatga attttatcta ccgctaacaa atcgtgatct ttgccgttga
     8161 tacgtagcgt aaaagagata cattttttac ccggacgatc aaagcaattg tatgccggat
     8221 tccagctgtt gttagagtca tctgaagcca ccgaccacgg tcgtttcgat gggttaaaat
     8281 cggcaaaaat aacccgccct gactcgtcga caagcggtcc atttacttgt tcctgatgat
     8341 aaacaacgat tttatcattc gccctccgcg aaaagagata atatttatta tcatcaagta
     8401 ccagtcccac cgagtctaaa aatggcatca tttccatttt cagaatcacg gcgcgttgaa
     8461 tctcctctgt attttttaat aaatacagtg agtcctttaa tgtcagggcc gaacgttcaa
     8521 tatctttaaa gaaatagcca acgtttttct gaataagttc aaagcggcta tcggcacgtt
     8581 ttaccgcgcg ttccgatacc agttgatagt taatcatgaa aaaaagacca ataaatgaca
     8641 gtgctgccaa taaaaataaa attatcagcc ttatcttttc gtataaactc agcatgttaa
     8701 tacatttaac aaaataacta tctgaacgct gtgccgctgc aaaaatagtc ccttgctgcg
     8761 gctttttaac caaattaccc gattctacaa aatcagcaga aagttacaac acttgcttag
     8821 ggcaataatt tcctgtgagg tgattaccct ttcaagcaat attcaaacgt aactatcctt
     8881 taattttcgg atccagcgca tcgcgtaaac catcgcccaa caaattgaac gccagtacgg
     8941 tcagaaaaat agccagggcc ggaaaaacag cgacatgcgg cgcgataacc atatccgctc
     9001 gtgcctcatt gagcattgct ccccactctg gtgtcggcgg ctgcgcaccg aggccgagaa
     9061 atgacaggct ggcggcagag ataatcgagg tgccaatgcg catggtgaaa aacaccacga
     9121 tagaagagac ggtcccaggc aggatatgac gcaataaaat ggtcatatcg ctggcaccaa
     9181 tactgcgtgc tgactcaata aaggtttgct gtttcaacac cagcgtgttg ccgcgcacca
     9241 ggcgggcaaa cgcggggatg gaaaaaatgg cgactgcgat aatcacgtta gcaatgccgc
     9301 ttcccaacac cgcaacaaca gcgatcgcca gtaaaatacc cgggaaggca aacagcacat
     9361 cgcaaatgcg catgatcagc cgatcccacc agccttcata atatccagcc aacaagccca
     9421 gcaacgtccc tatcgccaca ccgataaaca cggcaaacac gcccgccgcc agcgagattt
     9481 gcgcaccaac caggacacgg ctgaaaatgt cgcgccccag tgaatcgacg ccaaaccagt
     9541 gctgcaaaga aggtccgtta ttcagattgt cataatcaaa ataattttcg gcgtcatagg
     9601 gagcgatcca gcgtgcaaaa atggccacca caatcaataa aataacgaat aatgcggcgg
     9661 tcatcgccat atgctgacgg cgaaatcgtc gccagaattc atgccacggt gtacgtacct
     9721 ggtcaggttt gaccagtggc atggcgttta acaccgcctg acgtcgccag ttaaatagtc
     9781 gcatccttac ttgtacctga tagccgggtt aatggcggcg taaagcacat ccaccactaa
     9841 gttgataaga ataaattcca gcgagaaaag cagaatttcc gcctgaatca ccgggtaatc
     9901 gcgcatttct acagagtcaa cgagtaagcg tccaagtccg ggccagttga aaactttctc
     9961 caccacgata gaaccaccga gcaaaaagcc aaactgtaag cccatcatgg tcactaccgg
    10021 gatcatcgcg ttgcgtagcc cgtgtttgag gacaacccag gtttcgctca cccctttcgc
    10081 cctcgcggta cgcatataat cttcgcttaa aacgtcgaca aatgacgcac gggtaaagcg
    10141 cgccatcacg gcggccactg ccgcgccgag cgtcagggag ggtaaaatgt agtgctgcca
    10201 actgtctgct cccacagtag gcagccagcc cagttcaacg gagaatacct gaattaaaaa
    10261 catccccagc gcaaatgccg gaaacgagat ccccgacacc gcaatggtca tactcaatcg
    10321 atccggccaa cgattacgcc agacggcggc gataattccc gccgccatac caaatatcac
    10381 cgcccagacc atactggtta tggtcagcca tagcgttggc ataaagcggc tggcaatctc
    10441 atcggcaacc ggacgacgcg acaccatcga caggccaaaa tccccctgca cagcattgct
    10501 gatatagtgc cagaactggt gatacagcgg ctgatccaac cccagctgct gacgcaccag
    10561 ttctataacc tgcgcatcag cttcgggccc ggcaatcaat cgcgccggat cgccgggcag
    10621 catgtggaca aataaaaaca ccagcaccga gacgataaac agcgtcggaa tcaaccccag
    10681 taatcgtttg ataacgtaat aaagcattcc actccctgcg ttgcttattg caaatccgcg
    10741 tcttcaaagc tgaagccggt atctggcatg atccaaaaac cggtcaggtt tttactgtgt
    10801 gccgacacca gtttttctac caccagcggg atccacggcg attcttgcca gatgatatcc
    10861 tgcgccgcct tatataagcg ggtcttttcc gccggatcat tcgttttcag tgcctgagcc
    10921 aggaagtcat ccacctgttt attgctgtaa aacgcggtat taaacagcgt cggtggccag
    10981 ttttgcgagg caaacagcgg cgatagtgcc cagtcggctt cgccggttga agccgaccag
    11041 ccagtgtaga acatccgcac gccactctct ttttgccctt taccttcaac ttctgccgcc
    11101 cgctgtccgg catccatcgc ggtcacctgg gctttaatcc cgacctgcgc taactgctgc
    11161 tgggtaaatt gcagcacttt ctgcgcggtg ctgtggttat gtgacgacca cagcgtggta
    11221 ctgaaaccgt tgggatatcc cgcctctttc agtaattcgc gcgctttcac tggatcgtaa
    11281 ggccacggtt tataactttg cgcgtaggcg atacttggcg gtaccacacc agtagctgga
    11341 gttgcatagc ccgcaaaggc aactttcacc agcgccggac ggttaatggc gtaattcagc
    11401 gcctcacgga ccttcgggtt atcgaacggc ttttgcgtca cgttcatact gatataacgc
    11461 tgcataattg acggactggc catcaactcg atatttttgt ttttctccag cagtgtggcc
    11521 tgctcgtagg gaatggggaa agcaaactgc gcttcaccgg tttgcagcat tgccgcgcgg
    11581 gtgttgttat ccgccaccgg acgccaggtt atgctgtcca gtttgggcaa tcctggctgc
    11641 cagtaacccg cgaatttttt caccttcaca aaatcggtct gattccaggt atccagttca
    11701 tacggtccgg ttcccaccgg atgaaaacca atctccttgc catatttttc cagcgctgtc
    11761 ggtgaaatca tcgcggtcgc cggatgggca agaatattaa taaacgctga gaacggctgt
    11821 ttgagggtaa tctttaccgt tgtcggatcg atcgcctccg ttttggcaat attcttatac
    11881 aggttatagc gtttaagatg attcgccgga tcgctggccc ggtccagatt cgcttttacc
    11941 gccactgcgt tgaaatcggt gccatcctgg aatttaattc cttcccgcaa tttcacggtg
    12001 taagtaatgc cgtcatcgga aacggtataa ctctccgcca gcacgttttt cagtttcatc
    12061 tctttatcca gaccgaacag cccctggtaa aacgatttcg ctacggcctg agataacgtg
    12121 tcattcgcgt catacggatc gagcgtggtg aaattcgatc ctaccgccac caccacatct
    12181 ttggcagcga atgcacaaga tgccatcaac gctgtcgcaa tgcccagcgc cactaaccca
    12241 ctacggtgta cagctcttgc cattttattc tccgcctgaa tgttatctac gcatgaatgc
    12301 gtattctgat tgtggttgtg cgacgtaatg ccccggcccg acgcattgca acgagacggc
    12361 tgccacctct tcgccacgca gatgaatatt gctgggaaga tcgtccgaca gcagtacacg
    12421 ctgcggtcgt tgtcgggacg gttcagcgac cggaactgcc gccagtaatt tacgcgtata
    12481 aggatgctgc gggttttcga agaccgcgca ccgtgaacca atttcaacaa tttgcccgag
    12541 atacatcacc gccacacgat gactaatccg ctctaccaca gccatatcgt gggagataaa
    12601 cagatacgca atgccgaaat cacgctggag atcgagcaac aagttgataa tctgcccgcg
    12661 aatagaaaca tccagcgccg aaacggcttc gtcggcaatg atcacttttg gattcaatgc
    12721 caacgcgcga gcaatgcaga tgcgctggcg ctgaccgccg gaaaactcat gcgggtaacg
    12781 ccaggcatgt tcaggtaaca ggcccacgcg ctccagcaac cacgcaacgc gtgcagccgc
    12841 gtctttacct ggcaataaac cgtgtacacg cagcggttcg ataatcgaat caccgatggt
    12901 ctgacgtggg tccagcgaag cgtaagggtc ctgaaaaata aactgaatat cccggcgcaa
    12961 tgcctgaagt ttgccaggtg acaaggtatc gattcgctga ccgttaaaga taatttcgcc
    13021 gccctgcgat tcgaccaggc gcagcaacgc ccgcccggta gtggatttac cgctgccaga
    13081 ctcgcccacc agcgatagcg tttcgccagg ccagagatca aaactgactt tctcaacggc
    13141 atgcacttcc cgcgttacgc gattcaacaa accgctgcgc aaagggaaac gggtgacaag
    13201 attacgcacc cgtaaaacag gttcgccatc caccaccgtt ttctgctcga tggggggttc
    13261 ctgtttcgct ggatgttcaa gcgatatcaa tgggaaacgt cggggataat ctaacccttt
    13321 catcgcacca agttgcggaa cagcagctaa cagcgcacgg gtgtaaggat gttgcggtgc
    13381 atgaaaaatc tgttcgacgg tacccgtttc caccgcctcg ccctgataca tcaccagtac
    13441 ccgatcggca atctctgcca ccacgcccat atcgtgagtg ataaagataa cgcccatcga
    13501 catctctttt tgcaatactt tgattaattg caggatctgc gcctgaatag tgacatccag
    13561 cgcggtggtt ggctcatcgg caatcagcac cgccgggcgg catgacagcg ccatcgcaat
    13621 catcactcgc tggcgcatcc cgccagagag ttgatgcgaa taacgtgaaa gaatggtttg
    13681 tgcctccgga atgcgtacct gatccagcat ccgcttcgcc tcgaccatcg cttcttcacg
    13741 actggcgttc tgatgcagac gaattgattc ggcaatctgt tcacccacag taaatactgg
    13801 gttcagcgat gtcatcggct cctgaaatat catcgccata tccgcaccgc gcacatggcg
    13861 catttgtgca gcgctctgct cgctaagttc aatcacatca cgactgcgcc gccgcaacag
    13921 cattttatcg cactgtacta aaccgcccgc ctgttccaac agacgcatca atgccagcgc
    13981 agtcactgac ttaccggagc cggattcgcc tacaattgcc agcgtctcac cgcgttgcag
    14041 actaaaagag agattgcgga ccgcagctat tttctgctgg tcctgcataa aggcaatatt
    14101 aagattttca accgccagca cattaccggc atcaagttca tcactgtgtg gcaacggtgt
    14161 cccctttttc acggtagata ccggtggttg gcgtatcgcc tgcgtagccc caggcgcgat
    14221 acattccttc ggtgttaaac ggtagcgcga cattcccttc atggtcgata gcgattaagc
    14281 caccgctacc gccaagcgca gggagttttt ccattactac ccgctcgcag gcttccgcga
    14341 gacttaatcc gccgtaatcc attaacgcgg cgatgtcata tgccgccagc gcgcggatga
    14401 agacttcgcc tgtgccggta caagaaaccg ccacactggc gttattggcg tagcatccgg
    14461 cacccactaa gggactatcg ccaactcgtc cgggtaattt attggtcatt ccgcctgtgg
    14521 acgtggctgc cgccagattg ccgtctaaat ccaacgccac ggcccccacg gtgcccattt
    14581 tctgtttttc atccagtggc gcaccgctat ggtcgaggac tgttgcccct tcctcgcgcg
    14641 ctgccagtag ttgttcataa cgcaaaggcg tggagaaaat ctccggtgag acgcgctcca
    14701 tgccatgagc aaacgcaaaa ttttctgccc cttcgccaat catcatcaca tgcgggcttt
    14761 gctccatcac cagccgggcg gcaagaaccg gattacgcag atgactaacg cccgccaccg
    14821 caccggcttt cagggtgtta ccatccatca cacaggcgtc cagttcatgg gtttcatcac
    14881 gcgtaaagac agcgccaatt ccggcgttaa acagtggaca ctcttccagc agacgcaccg
    14941 cttccgtcac cacatccagc gcactttcgc cggcttccag cattttctgc ccggtttcaa
    15001 caatggcaga caacgcctcg atgtagcgta attcctgttg cagactcatc tgcgcgcggc
    15061 taattgcacc tgcgccacca tgaattgcaa tgactgcttt gcccatagta ttcgtccatt
    15121 aagggtggct cgccactttt gctataaata tcagaatttt tatgaattac tcattcaatt
    15181 tttgaatata gaaagatgga tatggcatgt aaaggcagga gccaacgcta cgcccgaggt
    15241 ggcatacagt ctatggcgtt tttctgacat aataggcaaa ttcgattttg cctccgcagg
    15301 agtgttttca tggaatttac caccggattg atgtcgctcg acaccgcgct taatgagatg
    15361 ctttctcgcg ttaccccact gaccacccag gaaacgctgc cactggttca gtgttttggt
    15421 cgtattctgg cgagcgatgt cgtttcgcca ctggatgtcc cggggtttga taactccgct
    15481 atggacggct acgcggtgcg tttagccgat attgcctccg ggcaaccgct gcccgttgcc
    15541 ggtaaatcct ttgccggtca gccataccat ggtgaatggc ctgcgggtac ctgcattcgt
    15601 attatgaccg gtgcgccggt gccggaaggc tgcgaagcgg tggtgatgca ggagcagact
    15661 gaacaaacgg acaatggcgt gcgttttact gctgaagtgc gtagcgggca aaatattcgc
    15721 cgtcgcggtg aagatatctc tgcaggtgcg gttgttttcc cggcgggaac tcgcctgact
    15781 accgcagagc tgccagtaat tgcttcactg gggattgccg aagttccggt gattcgtaaa
    15841 gtgcgtgtag cgcttttttc taccggtgat gaactccagt tgcccggtca gccgctgggc
    15901 gacggccaaa tctacgacac caaccgtctc gccgtacacc tgatgttaga acagctgggt
    15961 tgcgaggtaa tcaacttagg gattatccgc gacgatccac atgccctgcg cgccgcattt
    16021 attgaagccg acagccaggc ggatgtagtg atcagttccg gcggtgtttc agtgggtgaa
    16081 gcggattaca ccaaaatgat tcttgaagag ctgggggaga tcgccttctg gaagctggcg
    16141 attaaaccag gtaaaccgtt cgcgttcggt aaactcagca atagctggtt ctgcggcctg
    16201 ccgggcaacc cggtttcagc gacgctgacc ttctatcaac tggtacagcc tttgctggca
    16261 aaactaagcg gcaacaccgc cagcggcctg cccgcgcgcc agcgcgtacg cacagcgtcc
    16321 cgcctgaaaa aaacgccagg acgtcttgat ttccagcgcg gcgtgctgca acgcaacgcc
    16381 gacggtgaac ttgaagtgac gaccaccgga catcagggtt cacatatatt tagctccttt
    16441 agtctcggca actgctttat cgtgctggaa cgcgatcgcg gcaatgtgga cgtgcgcgaa
    16501 tgggtggaag tagaaccgtt taacgcgttg ttcggaggcc tgtaatggcg gaactcagcg
    16561 atcaggagat gctgcgctac aaccggcaaa tcattctgcg cggttttgat tttgacggtc
    16621 aggaggcgct aaaagattct cgtgtgctgg tagtcggcct cggtggactc ggctgcgcag
    16681 cttcgcagta tctggcaagc gccggtgtcg gcaacctgac gctgctcgac ttcgacacgg
    16741 tttcgctctc gaatctgcaa cgccagacac tgcacagtga tgccacggtc gggcaaccga
    16801 aggtggaatc cgcccgtgac gccctgacgc ggatcaaccc acatatcgcg attacgccag
    16861 tcaatgcact gctagatgac gcagaacttg cagcattgat tgctgaacac gatctggtgc
    16921 tcgactgtac ggataacgtt gcggtacgta atcaactgaa cgcaggctgt tttgccgcga
    16981 aggtgccgct ggtttccggc gcggcaattc gtatggaagg gcaaatcacg gtcttcactt
    17041 atcaggacgg cgaaccgtgc tatcgctgcc ttagccgttt gtttggcgag aacgcgttaa
    17101 cctgcgtaga agcaggcgtg atggcaccgt tgataggcgt aattggttcg ttgcaggcaa
    17161 tggaagcgat caaactgctg gcaggttatg gcaaaccggc cagcgggaaa atcgtcatgt
    17221 acgatgcgat gacctgtcag tttcgcgaaa tgaaactgat gcgcaatccg gggtgtgagg
    17281 tgtgcgggca gtaattgcca cctccaagcc tgataagacg cgacaagcgt cgcatcaggc
    17341 atttgctcca gacgccagat gcggcgtgaa cgccttatct ggcctacggt tcgggcacag
    17401 atttccaagc gcggcaggca tcggtgcact ccgccacgtc atcagcaatt tatgaggatg
    17461 cagaacgtcc ggttaaatcg acgttctgcc aaacgctccc tgccagtcct gctcaaactt
    17521 cgtcacagcg gcttccaccg ccggatagct aatcatctgt tgtgccacat ccagtggcag
    17581 agtaattgat tcacatccgg ccagtaagca gtccagcgcc tgacgcgggg ttttgaaact
    17641 cgctgccagc actttcgcct gcggcgcatg cattttcaat aactggtgta agtcggtcac
    17701 agtctgaatg ccgctaccgc cctgagcatc aatacgatta acgtaaggcg caacatattc
    17761 cgcacctgcc agcgccgaca gcagcccttg tgccgcgcca tataccgcgg ttcccagcgt
    17821 cggaatccct tccgctttta acatcttaat cgctgccagc ccctcggctg tcaccggaac
    17881 tttcaccacg atatccgcaa taatagaacg cagcttaagc gcgtcattca ccatcccttc
    17941 agcagtggta gccattacct gggcaaacag acgcccctga ccgcccatcg cttcatgaag
    18001 ttgcggaagc acaacatcca gcggtttttt acccgcggcg ataatgcttg ggttagtggt
    18061 cacacccgcc agcggaaaaa tacgtgacag cgccttcacc gcaacaacgt ctgaagtatc
    18121 cagatacagt tccataacca tcctcaaaat gtactttttg tctgaatgta gactatcacg
    18181 ggaaaactct ctgaacagtt gacgcacatc aatctaactt tcattcgaaa gtattttaat
    18241 gtttgaacga aacagagagg caactatgat tttcaatatt cagcgctact cgacccatga
    18301 tggccccggt atccgcacgg tcgtatttct taaaggctgt tcgctgggct gccgctggtg
    18361 tcagaacccg gaaagccgcg cccgtacgca ggatctgctg tatgacgcac gactgtgtct
    18421 ggaaggctgc gagctgtgcg ctaaggccgc gccggaagtg attgagcgcg cgctgaatgg
    18481 tttgcttatt catcgggaaa agttaacccc ggagcatctg acggcgttaa ccgactgctg
    18541 tccgacacag gcgttaaccg tgtgtggtga agtgaaaagc gttgaggaga tcatggcgac
    18601 cgttctgcgc gataaaccgt tttacgatcg cagcggcggc ggcttaacgc tttcgggtgg
    18661 tgaacccttc atgcagccgg aaatggcgac ggcgctgctg caagcctgcc acgaggcagg
    18721 cattcatact gcggtagaaa cctgtctgca tgtgccgtgg aaatatatcg ccccttctct
    18781 gccctatatc gatctgtttc ttgccgattt aaaacacgtt gccgacgcgc cgtttaaaca
    18841 gtggaccgac ggtaacgccg ccagggtgct ggataacctg aaaaaactcg ccgcagcggg
    18901 caaaaaaatc attatccgcg tgccgctgat tcagggcttt aatgccgacg aaacctctgt
    18961 aaaagccatt accgattttg ccgccgacga gctgcacgtt ggcgaaattc attttctgcc
    19021 ctaccacacg ctgggcatca acaaatatca cttacttaat ctgccctatg acgccccgga
    19081 aaaaccgctt gatgcgccag agctgctcga ttttgcccag cagtatgcct gccagaaagg
    19141 gttaaccgcg accttacgag gataacaacc atgaccacac tgaaactgga cacgctcagc
    19201 gaccgcatta aagcgcacaa aaatgcgctg gtgcatattg tgaagccgcc agtctgtacc
    19261 gagcgcgcgc agcactatac cgagatgtat caacaacatc tcgataagcc gatcccggta
    19321 cgtcgcgcgc tggcactggc gcatcacctg gcgaatcgca ccatctggat caaacacgat
    19381 gagttgatca ttggcaacca ggcaagcgaa gttcgcgccg cgccgatctt cccggaatat
    19441 actgtctcgt ggatcgaaaa agagattgat gatctggcag atcgtcccgg tgctggcttt
    19501 gcggtgagcg aagagaacaa acgtgttctg catgaagtgt gcccgtggtg gcgcggtcag
    19561 acggtacagg atcgctgcta tggcatgttt accgatgagc aaaaaggtct gctggcgacc
    19621 ggcatcatta aagcggaagg caatatgacc tccggcgatg cgcacctggc ggtcaatttc
    19681 ccgctgctgc tggaaaaagg gcttgatggt ctgcgtgaga aagtagcgga acgtcgctcg
    19741 cgcatcaatc tgacggtgct ggaagatttg cacggcgaac agttcctgaa agcgattgat
    19801 atcatgctgg tggcagtcag tgaacacatt gaacgtttcg ctgccctggc gcgtgcaatg
    19861 gccgcgaccg aaacccgcga aagccgtcgc gatgaactgc tggcgatggc agaaaactgc
    19921 gatcttatcg cccaccagcc gccgcagact ttctggcagg cgctgcaact gtgttacttc
    19981 atccagttga ttttgcagat tgaatctaac ggtcactcgg tgtcgtttgg tcgtatggat
    20041 cagtatctct acccgtacta tcgccgcgac gttgaactga accagacgct ggatcgcgaa
    20101 cacgccatcg aattgctgca tagctgctgg ttgaaattgc tggaagtgaa caaaatccgc
    20161 tccggctccc actcaaaagc ctctgcggga agtccgctgt atcagaacgt caccattggc
    20221 ggacaaaatc tggttgatgg tcaaccaatg gacgcggtga atccactctc ttacgcgatc
    20281 ctcgaatcct gcggtcgcct gcgttccact cagcctaacc tcagcgtgcg ttaccatgca
    20341 ggaatgagta acgatttcct cgacgcctgc gtacatgtga tccgctgcgg cttcgggatg
    20401 ccggcattca acaacgacga aatcgtgatc ccggaattta ttaaactcgg tattgaaccg
    20461 caggacgctt atgactacgc agcgattggt tgtatagaaa ccgccgtcgg tggcaaatgg
    20521 ggctatcgct gtaccggcat gagctttatc aacttcgccc gcgtgatgct ggcggcgctg
    20581 gaaggcgggc gtgatgccac cagcggcaaa gtgttcctgc cacaagaaaa agcgttgtcg
    20641 gcaggtaact tcaacaactt cgatgaagtg atggacgcgt gggatacgca aatccgttac
    20701 tacacgcgta aatcaatcga aatcgaatat gtcgtcgaca ccatgctgga agagaacgtg
    20761 cacgatattc tctgctcggc gctggtggat gactgtattg agcgagcgaa aagtatcaag
    20821 caaggcggcg cgaaatatga ctgggtttct ggcctgcagg tcggtattgc caacctcggc
    20881 aacagcctgg cggcagtgaa gaaactggtg tttgaacagg gcgcgattgg tcagcaacag
    20941 ctggctgccg cactggcgga tgacttcgat ggcctgactc atgagcaact gcgtcagcgg
    21001 ctgattaacg gtgcgccgaa gtacggcaac gacgatgata ccgtcgatac gctgctggct
    21061 cgcgcttatc agacctatat cgacgaactg aaacagtacc ataacccacg ctacggtcgt
    21121 ggtccggttg gcggcaacta ttacgcgggt acgtcgtcta tctccgctaa cgtaccgttt
    21181 ggcgcgcaaa ctatggcaac gccggacggg cgtaaagccc acaccccgct ggcagaaggc
    21241 gctagtccgg cctctggtac tgaccatctc ggccctactg cggtcattgg ctcagtgggt
    21301 aaactgccta cggcagcgat tctcggcggc gtgttgctca accagaaact gaatccggca
    21361 acgctggaga acgaatctga caagcagaaa ctgatgatcc tgctgcgtac cttctttgaa
    21421 gtgcataaag gctggcatat tcagtacaac atcgtttccc gcgaaacgct gctggaggcg
    21481 aaaaaacatc cggatcagta ccgcgatctg gtagtgcgtg tcgcgggtta ttccgccttc
    21541 ttcaccgcgc tctctccaga cgctcaggac gatatcatcg cccgtactga acatatgctg
    21601 taatctcttc ggcccggctg cggcgtcgcc gggctaaatc attcacatca tcaatttcat
    21661 ccttactttc attcgaaata taatttgtgc tctgcgtcac attgttatta cactgttacg
    21721 gtttcatttt cgagccagga gcacacctat gagcgtaaaa gttatcgtca cagatatgga
    21781 cggtactttt cttaacgacg ccaaaacgta caaccaacca cgttttatgg cgcaatatca
    21841 ggaactgaaa aagcgcggca ttaagttcgt tgtcgccagc ggtaatcagt attaccagct
    21901 tatttcgttc tttcctgagc taaaggatga gatctctttt gtcgcggaaa acggcgcact
    21961 ggtttacgaa catggcaagc aactgttcca cggcgaactg acccgacatg aatcgcggat
    22021 tgttattggc gagttgctaa aagataagca actcaatttt gtcgcctgcg gtctgcaaag
    22081 tgcatatgtc agcgaaaatg cccccgaagc atttgtcgca ctgatggcaa aacactacca
    22141 tcgcctgaaa cctgtaaaag attatcagga gattgacgac gtactgttca agttttcgct
    22201 caacctgccg gatgaacaaa tcccgttagt gatcgacaaa ctacacatag cgctggatgg
    22261 cattatgaaa cccgtcacca gtggttttgg ctttatcgac ctgattattc ccggtctaca
    22321 taaagcaaac ggtatttcgc ggttactgaa acgctgggat ctgtcaccgc aaaatgtggt
    22381 agcgattggc gacagcggta acgatgcgga gatgctgaaa atggcgcgtt attcctttgc
    22441 gatgggcaat gctgcggaaa acattaaaca aatcgcccgt tacgctaccg atgataataa
    22501 tcatgaaggc gcgctgaatg tgattcaggc tgtgctggat aacacatccc cttttaacag
    22561 ctgacctctc taccggagcc atgcgctccg gttttccctt ctttttacat cctgtacatc
    22621 acatcaaatc attaaacttg cattaactta tttataactt gtattgtcac atgcaaatta
    22681 gcatagtttc gaatgaaagt taatgcaagg tggttatggc ttctactttt accagcgaca
    22741 cattgcctgc cgatcacaaa gcagctatcc gtcagatgaa gcacgcgctg cgggcgcagc
    22801 ttggcgatgt ccagcagatc tttaatcagc taagcgatga cattgccacg cgagtggctg
    22861 aaatcaacgc actcaaagca cagggcgatg ccgtctggcc ggtgctgtct tatgccgata
    22921 tcaaagcagg tcatgttact gcagagcagc gcgaacagat taaacgtcgc ggttgtgcgg
    22981 tgataaaagg ccatttcccc cgcgaacaag cgctaggctg ggatcagtcg atgctggact
    23041 atctgaaccg caaccgcttt gacgaggtct acaaaggccc cggcgataat ttcttcggga
    23101 cgctcagcgc ttcacgtccc gagatttacc ccatctactg gtcgcaggcg caaatgcagg
    23161 cccgccagag tgaagaaatg gcgaatgcgc agtcgtttct caatcgtctg tggacatttg
    23221 aaagtgatgg aaagcaatgg tttaacccgg atgtgagcgt catctaccct gaccgtatcc
    23281 gccgccttcc gcccggaacg acctccaaag gtcttggagc gcataccgac tccggggcgc
    23341 tggaacgctg gctgcttcca gcgtatcagc acgttttcgc caacgtcttt aatggcaatc
    23401 tggcgaaata cgatccctgg catgcggcac atcgtacgga agttgaagag tacacggtgg
    23461 acaacaccac caaatgttcc gtgtttcgga cattccaggg ctggactgcg ctctcggata
    23521 tgctgcctgg tcaggggttg ctgcacgtcg tgcccattcc tgaagccatg gcgtacgtac
    23581 tgttacgtcc gctgcttgat gatgtgccgg aggatgaact gtgcggcgta gcgcccggaa
    23641 gagtgttgcc ggtatcagag caatggcatc cactgttaat tgaggcgtta accagcattc
    23701 caaaactcga ggccggagac tccgtctggt ggcactgcga cgtcatccat tccgttgccc
    23761 ccgttgaaaa tcaacagggt tggggcaacg tgatgtacat tcctgcggca ccgatgtgcg
    23821 agaaaaatct tgcctacgcg cacaaggtga aggccgcact ggaaaaaggc gcatcgccgg
    23881 gcgacttccc gcgcgaggac tatgaaacaa actgggaagg acgctttacg cttgccgacc
    23941 tcaacattca cggtaagcga gcgttgggca tggatgtttg agagttaatg gaagtgaatt
    24001 gatgtgggat taagaaaatc gattcgacgt tactcagatt gctgacaagt gcgctttctt
    24061 aatgcctgat gcggcgttaa cgccttatcc ggcctacaaa gtcctgcaga ttcaatatat
    24121 tacaggtact gcgtaggcct gataagcgta gcgcatcagg ctattttgca tttgtcatca
    24181 gccgtcgtca ccccgacagt atcggggtga cggataatca cttactcgat ccctttacta
    24241 cgcaggtaat cttcgtaatt accgctaaag tcgatcacgc gttccggggt gatttccaga
    24301 atgcgggtcg ccagggagct tacgaactca cggtcgtgtg aaacaaagat cagcgtgccc
    24361 tgatacagtt ccagcgccat gttcagtgac tcgatggatt ccatatccag gtggttggtc
    24421 ggttcgtcca tgatcagaat gttcggcttc tgcatcatta acttaccaaa cagcatccgc
    24481 cctttttccc caccggaaag cactttagct ggctttttga tgtcgtcctg gctgaacagc
    24541 aaacgaccga gaatgctgcg taccgcctgc tcgtcatcgc cttcctgctt ccactgactc
    24601 atccattcga acacggtcag atcattttca aactcatatt cgtggtcctg ggcgtagtaa
    24661 ccaatgcgcg cgttttcaga ccattttacg gtgccgctgt ccggttgcag atcgcccacc
    24721 agcgttttca gcagcgttga tttaccgacg ccgttggtac ccagtaccgc aagtttttca
    24781 ccgacctcca gcagcaggtt gagattttta aacagtggac cgttatcaaa ccctttggtc
    24841 agaccttcca cttccagcgc gttacggaac agtttcttat cctgttcaaa acggataaac
    24901 gggttctgac ggctggaggc tttcacctct tccagtttga ttttatcaat ctggcgcgcg
    24961 cgcgaagttg cctggcgaga tttcgaggcg ttggcgctaa agcggctaac gaaagattgc
    25021 aactcagcaa tctgcgcttt cttcttggcg ttatcggcca gcagacgttc acgcgcctgg
    25081 gtcgccgccg tcatgtactc atcgtagtta cccggataaa cgcgcagctc gccgtaatcc
    25141 agatccgcca tgtgggtaca gaccatgtta aggaagtgac ggtcgtgcga gatgatgatc
    25201 atggtgctgt cacgctcgtt cagcacctgt tccagccagc gaatggtgtc gatgtcgagg
    25261 ttgttggtcg gttcgtcgag caggagaata tccggctcag caaacagcgc ctgcgccaga
    25321 agcacacgca gcttccagcc aggagcaact tcactcatcg ggccgtagtg ctgttccact
    25381 ggaattccca cgccaagcaa cagttcaccg gcgcgagctt ccgcagagta accgtccatt
    25441 tcgccgtatt tcacttccag atcggccact ttatagccgt cttcttcact catttccggc
    25501 aaagcataga tgcggtcgcg ctcctgcttc acttcccaca actctttatg ccccatgatc
    25561 accgtatcca gcacagtgaa ctcttcaaag gcaaactgat cctgacgcag cttaccaatg
    25621 cgctcgttgg gatcgaggga aacgttaccc agcgtcggct caaggtcgcc gccgagaatc
    25681 ttcataaagg tggatttacc actaccgttc gcgccaatca ggccgtaacg gttgccgccg
    25741 ccgaatttga cggaaatgtt ttcaaacaac ggcttactgc cgaactgcat ggtgacgtta
    25801 ctggaaacta acacaggcgt atcctgaaaa gagatatgac aaaccgcgca ttatgccata
    25861 agtctgaaag aagatcctgc tcctcgctta aggtctacac tcaagccaaa ggcaaaaaat
    25921 gtgatttcgt acacatctga tttcactgtg agctggaatg aacttataat gcgcttccaa
    25981 tactctaata ttctcaaccc aatggcctgc caggcacaaa atctcgctta acatgaatat
    26041 gaaattgaaa acattattcg cagcggcctt cgctgttgtc ggcttttgca gtaccgcctc
    26101 tgcggtaact tatcctctgc caaccgacgg gagtcgcctg gttggtcaga atcaggtgat
    26161 caccattcct gaaggtaaca ctcagccgct ggagtatttt gccgcggagt accagatggg
    26221 gctttccaat atgatggaag cgaacccggg tgtggatacc ttcctgccga aaggcggtac
    26281 tgtactgaac attccgcagc agctgatcct gccggatacc gttcatgaag gcatcgtcat
    26341 taacagtgca gagatgcgtc tgtattacta tccgaaaggg accaacaccg ttatcgtgct
    26401 gccgatcggc attggtcagt taggcaaaga tacgcctatc aactggacca ccaaagttga
    26461 gcataagaaa gcaggcccga cctggacgcc gaccgccaaa atgcacgcag agtaccgcgc
    26521 tgcgggcgaa ccgctaccgg ctgtcgttcc ggcaggtccg gataacccga tgggtctgta
    26581 tgcactctac atcggtcgcc tgtatgctat ccatggcacc aacgccaact tcggtatcgg
    26641 cctgcgtgta agtcatggtt gtgtgcgtct gcgtaacgaa gacatcaaat tcctgttcga
    26701 gaaagtaccg gtcggtaccc gcgtacagtt tattgatgag ccggtaaaag cgaccaccga
    26761 gccagacggc agccgttata ttgaagtcca taacccgctg tctaccaccg aagcccagtt
    26821 tgaaggtcag gaaatcgtgc caattaccct gacgaagagc gtgcagacag tgaccggtca
    26881 gccagatgtt gaccaggttg ttcttgatga agcgatcaaa aatcgttccg ggatgccggt
    26941 tcgcctgaat taatcgtcaa aacgtaaaac aaaggcggac tcataatccg ccttttttat
    27001 ttgccagacc ttaccaggcc gggatcataa ctaacaaaac gtatcccacc agcgccgccc
    27061 acaacagcat cggtattgaa tagagatgga aacgccacca gatgcggcga tcgttcgcca
    27121 tacgtagcgc aatcaaattt gccagcgatc ctggtaataa accaaagccc cccacgttta
    27181 ccgcccatgc cagtaataaa gacggaggta cataattgag cagcaaaatg gtactcggca
    27241 cgttactgat cacctgcgat aaaccgactg ccgttaacca taacccgggt tcagataaat
    27301 gactcacgtt acccaacacg ccttgcaacg ctggaagctg ggtcagtaaa tggacgtcga
    27361 taaacatcgc cataaacacc agcagcaacg tccagtccac actcaacacc acgcgacgcg
    27421 ccagcagcac aaagcccgcc gccacaatca ccagtcccca cagctcttgt ttgaactcca
    27481 gcgccgtcag aaagacgata tacagcccca gacaattcca caccagccgc ggtttccact
    27541 ccggtgtttg cacccccgta tggtattgca gcgcctttcc agggaaacaa caccagcaca
    27601 acagcaggag tgtcagcatc attgcgccag ccatcggtgc catttgggca ataaatccgg
    27661 caaacgaaag accagaacgt ccccagataa gaatattttg cgggttgcca attggcgtca
    27721 gtagcgaacc agcgttgact gccagcgcct caaaaataat cagccgatta accgggatct
    27781 cacacagtct ttttagcgtg atagtcagcg gaacaacaat aaacagcgcg acatcgttgg
    27841 tcagaaaggt agaaagcagc gccgccgcca gcaccataaa catcgccagc cgacgctccg
    27901 tagcaaagcg gcgcaccatt ttgcgcccca gcacatcaaa ataaccgctt aactccacac
    27961 ctttggtcag cagcatcagg ccgcttaagg tgatgatggt gtgccagtcg atagcagcag
    28021 gccaggattt cggtgcaaag ggcacgaaaa agcttaatcc gataccaaca agaattaata
    28081 actgaaaaaa acgatcgcct tgcagcgtgc gtaaaaaagg caggctcatt tggcaccgtg
    28141 tttctgggta aacaaacgaa aagcgtcgag cgtctcttca ctaacatggt gctccatgcc
    28201 ttccgcgtcg cgacgggcga tttccggact gacgcccaac accaacaaga aattttcgac
    28261 tatctgatgt cgctcgcggc tttcctgcgc cagcttctct ccttctgccg ttaaaaacac
    28321 gccacgccag gggatcattt caatcagccc catggttgcc agccgcttaa gcattttagc
    28381 caccgtcggt tgcgaaactc ccagacgagc agccatgtcc acctgacgag cttcccccac
    28441 ttccctgatc aagtcagaaa tcagctcaac gtaatcatca ataagctcgc gccgatgcgc
    28501 ctctctgacc tggcggaacc cttcaacgtg ctcttccacg ttcactaact gcgtcacttt
    28561 ttttgctatt ggcgtacctg cgcgacgact cattgtgctt cctcattatg gtgacgcata
    28621 catagcgttc tcatttcgtg cgcacattgt aaaccagagt tgcgaaggta caaaaaatta
    28681 acgttttagc aatagctata taatatagcc tgtgctatat ctgtatgtaa tgcaatcatc
    28741 cctcaaggat cgacgggatt agcaagtcag gaggtcttat gaatgagttc aagaggtgta
    28801 tgcgcgtgtt tagtcattct ccctttaaag tacggttaat gctgctctct atgttgtgcg
    28861 atatggtcaa caacaaaccg cagcaagata aaccttcaga taaatagcgg cgtcgcggta
    28921 cgccgcttca ctcctgcttt tatgcaggca taacgcgttt tggtctgaaa aaaaccactt
    28981 tttgtcggat ttgcaatccc cttcgcaaaa gatttgttcg tcagtagttg acctgaacgg
    29041 cggctcgctc tatcttcttg cagccctgcg tatattgcgg ctcgcggatg cggacccctt
    29101 tcccctcttc acgcactctt gcaggtattg acccttgacg ccagggtaag cacatggcgt
    29161 ttgttacgat agtggcatat gaatttaacc ctcaaagaat cgcttgttac ccgtagccgg
    29221 gtatttagcc cgtggactgc gttctacttt ttacagtcgc tattaattaa cctcggctta
    29281 ggttacccct tcagtttgct ctacaccgct gcgtttacgg ctattttgct tttgctatgg
    29341 cgaacattgc ctcgcgtaca aaaagttctg gtcggtgtca gttcgctggt ggcggcttgt
    29401 tatttccctt ttgctcaggc ctacggcgcg cctaatttca atacattgct ggcattgcac
    29461 tccaccaata tggaagagtc gaccgaaatc ctgacgattt ttccgtggta cagctacctg
    29521 gtcggcttat ttatttttgc gctcggcgta atagcaatca ggcgaaaaaa agagaatgaa
    29581 aaagcgcgct ggaatacctt cgacagcctg tgtctggtat tcagtgtggc gacatttttt
    29641 gttgctcccg tgcaaaacct ggcctggggt ggcgtattta aactgaaaga tactggctat
    29701 ccggtatttc gttttgctaa ggatgtcatc gtcaataata acgaggtgat tgaagagcaa
    29761 gaacggatgg caaaactttc cggaatgaaa gatacctgga cggtcactgc cgttaagccg
    29821 aagtatcaga cctatgtggt agtgatcggt gaaagcgcgc gtcgcgatgc cctcggtgcc
    29881 tttggcggtc actgggacaa taccccgttt gccagcagcg ttaacggttt gatatttgct
    29941 gactacattg ctgccagtgg ctccacgcag aaatcgcttg gcttaacgct caatcgcgta
    30001 gtcgatggca aaccacagtt tcaggataac tttgtcaccc tggcaaatcg cgcgggcttc
    30061 cagacctggt ggttttccaa ccagggccaa atcggcgaat acgatactgc tatcgccagc
    30121 atcgccaaac gtgcagatga agtgtacttc ctgaaagaag gtaattttga agcagataaa
    30181 aacacgaaag acgaagcgtt actggatatg accgctcaag tgctggcgca agagcactcg
    30241 caaccgcagc tgattgttct gcatctgatg ggctcgcatc cgcaggcctg cgacaggaca
    30301 caaggcaaat acgaaacctt tgtgcaatcg aaagaaacgt cgtgctatct ctataccatg
    30361 acgcaaacgg acgatttact gcgcaagctg tacgatcagt tacgcaacag cggcagcagc
    30421 ttctctctgg tttacttttc tgaccacggt ctggccttta aagagcgcgg caaagacgtg
    30481 caataccttg cccatgatga taaatatcag caaaatttcc aggtgccttt tatggtcatt
    30541 tccagcggcg ataaagcgca tcgggtgatt aaagcccgcc gctcagccaa tgacttctta
    30601 ggctttttct cgcagtggac gggaattaaa gcgaaggaaa taaatatcaa atacccgttt
    30661 atatctgaga agaaagccgg gccgatatac atcaccaact tccagttaca gaaggtggat
    30721 tacaaccatc tcggaaccga tattttcgac ccgaaacctt aaaaacaaaa atccgccccg
    30781 agaggcggat ttttatatca ccaaagtgat tagaagcggt aaccaacacc ggcaatccag
    30841 gtgcctacgt caacgctacg aatacggctc tgctcgtaag agaagtccag agcaacgttt
    30901 tccatcgggt tgaactgcag accagcaccg taggagaaac cgtagtcgct ggtgtcgtgt
    30961 ttgtaggtcg ggtattcagt ggtctggaat ttaccataac ccacacccac tacaccgtag
    31021 atgcttgccc agtcgttaat gcggtaagcc ggaccagcag tgatgccgta gtactggttt
    31081 ttgttgtagt caccagagct tgcagtacgg cttttctcgg tgtaagtgaa agaaccgatc
    31141 acacccagcg ggctgttgtc ttcttcatag cggtatttca ggttgaaacc gcccattttg
    31201 ttcatttggc cctgagcgtc gctctgtgcg taaccgccag ttacggtaga agtcgcagct
    31261 acggaagtac ctgcggtgaa agccagaact gcggccagtg ctgaaagaca tgcaattttt
    31321 ttcataacca cctcaaatgt gattcaaata agtcctaagt tttaaatata tcaaaaatta
    31381 atgggaaact cttcgcgatt tgtgatgtct aacgggccat ttcatgtaac agaacgtttc
    31441 catacaccgc tatccatcta aatttaaatc actttttcag agaactgcgt aagtattacg
    31501 catgttttcc ctgtcattca tccagattat tcctaatcac cagactaatg attccatcaa
    31561 tcctggcgca ttttagtcaa aacgggggaa aattttttca acaaatgctc aaccagcatt
    31621 gggtatatcc agtacactcc acgctttact taagtctaga tatttgtggg agaaaggatg
    31681 cctggttcat tacgtaaaat gccggtctgg ttaccaatag tcatattgct cgttgccatg
    31741 gcgtctattc agggtggagc ctcgttagct aagtcacttt ttcctctggt gagcgcaccg
    31801 ggtgtcactg cgctgcgtct ggcattagga acgctgatcc tcatcgcgtt ctttaagcca
    31861 tggcgactgc gctttgccaa agagcaacgg ttaccgctgt tgttttacgg cgtttcgctg
    31921 ggtgggatga attatctttt ttatctttct attcagacag taccgctggg tattgcggtg
    31981 gcgctggagt tcaccggacc actggcggtg gcgctgttct cttctcgtcg cccggtagat
    32041 ttcgtctggg ttgtgctggc ggttcttggt ctgtggttcc tgctaccgct ggggcaagac
    32101 gtttcccatg tcgatttaac cggctgtgcg ctggcactgg gggccggggc ttgttgggct
    32161 atttacattt taagtgggca acgcgcagga gcggaacatg gccctgcgac ggtagcaatt
    32221 ggttcgttga ttgcagcgtt aattttcgtg ccaattggag cgcttcaggc tggtgaagca
    32281 ctctggcact ggtcggttat tccattgggt ctggctgtcg ttattctctc gaccgctctg
    32341 ccttattcgc tggaaatgat tgccctcacc cgtttgccaa cacggacatt tggtacgctg
    32401 atgagcatgg aaccggcgct ggctgccgtt tccgggatga ttttcctcgg agaaacactg
    32461 acacccatac agctgctggc gctcggcgct atcatcgccg cttcaatggg gtctacgctg
    32521 acagtacgca aagagagcaa aataaaagaa ttagacatta attaaattta catttctgta
    32581 tggttatgca taaccatgca gaatttctcg ctacttttcc tctacaccgt atttatatat
    32641 cgaattatgc aaaagcatat ttattccgaa aattcctggc gagcagataa ataagaattg
    32701 ttcttatcaa tatatctaac tcattgaatc tttattagtt ttgtttttca tgcttgttac
    32761 cactattagt gtgataggaa cagccagaat agcggaacac atagccggtg ctatacttag
    32821 tctcgttaat tactgggaca taacatcaag aggatatgaa attatgagta ccgctaaatt
    32881 agttaaatca aaagcgacca atctgcttta tacccgcaac gatgtctccg acagcgagaa
    32941 aaaagcaaca gtagagttgc tgaatcgcca ggttatccag tttattgatc tttctttgat
    33001 taccaaacaa gcgcactgga acatgcgcgg cgctaacttc attgccgtac atgaaatgct
    33061 ggatggcttc cgcaccgcac tgatcgatca tctggatacc atggcagaac gtgccgtgca
    33121 gctgggcggt gtagctctgg ggaccactca agttatcaac agcaaaaccc cgctgaaaag
    33181 ttacccgctg gacatccaca acgttcagga tcacctgaaa gaactggctg accgttacgc
    33241 aatcgtcgct aatgacgtac gcaaagcgat tggcgaagcg aaagatgacg acaccgcaga
    33301 tatcctgacc gccgcgtctc gcgacctgga taaattcctg tggtttatcg agtctaacat
    33361 cgaataaatc catcgctgat ggtgcagaac tttagtaccc gataaaagcg gcttcctgac
    33421 aggaggccgt tttgttttgc agcccacctc aacgcactta tttagtgcat ccatctgcta
    33481 tccccagctg attaagtaaa ttttttgtat ccacatcatc acacaatcgt tacataaaga
    33541 ttgttttttc atcaggtttt acgctaaata atcactgtgt tgagtgcaca attttagcgc
    33601 accagattgg tgccccagaa tggtgcatct tcagggtatt gccctataaa tcgtgcatca
    33661 cgtttttgcc gcatctcgaa aaatcaatga gttgcaaaat tggcacgatt ttttcatata
    33721 tgtgaatgtc acgcagggga tcgtcccgtg gatagaaaaa aggaaatgct atgaagtctg
    33781 tattaaaagt ttcactggct gcactgaccc tggcttttgc ggtttcttct catgccgcgg
    33841 ataaaaaatt agttgtcgcg acggataccg ccttcgttcc gtttgaattt aaacagggcg
    33901 ataaatatgt gggctttgac gttgatctgt gggctgccat cgctaaagag ctgaagctgg
    33961 attacgaact gaagccgatg gatttcagtg ggatcattcc ggcactgcaa accaaaaacg
    34021 tcgatctggc gctggcgggc attaccatca ccgacgagcg taaaaaagcg atcgatttct
    34081 ctgacggcta ctacaaaagc ggcctgttag tgatggtgaa agctaacaat aacgatgtga
    34141 aaagcgtgaa agatctcgac gggaaagtgg ttgctgtgaa gagcggtact ggctccgttg
    34201 attacgcgaa agcaaacatc aaaactaaag atctgcgtca gttcccgaac atcgataacg
    34261 cctatatgga actgggcacc aaccgcgcag acgccgttct gcacgatacg ccaaacattc
    34321 tgtacttcat caaaaccgcc ggtaacggtc agttcaaagc ggtaggtgac tctctggaag
    34381 cgcagcaata cggtattgcg ttcccgaaag gtagcgacga gctgcgtgac aaagtcaacg
    34441 gcgcgttgaa aaccctgcgc gagaacggaa cttacaacga aatctacaaa aaatggttca
    34501 gtactgaacc gaaataataa cgctacacct gtaaagcgca ctggcagttc cctctccccc
    34561 ctggggagag gattagggtg aggggcgcaa acccgctccg gggccattaa ttaccctgaa
    34621 tttgattatt tacaccacgg taacaggaac aacatatgca gtttgactgg agtgccatct
    34681 ggcctgccat tccgcttctg attgaaggtg ccaaaatgac cctgtggatt tcggtcctcg
    34741 gtctggctgg cggtctggta atcggattgc tggcaggttt tgcacgcacc ttcggtggtt
    34801 ggatagccaa ccacgtcgcg ctggtcttta ttgaagtgat ccgcggcaca cctatcgtcg
    34861 tccaggtgat gtttatctat ttcgccctgc cgatggcgtt taacgactta cgcatcgacc
    34921 catttactgc ggcggtggtc accatcatga tcaactccgg cgcgtacatt gcggaaatca
    34981 cgcgtggtgc ggtgctgtct atccacaaag gttttcgtga agcaggactg gcgctcggtc
    35041 tttcacgttg ggaaaccatt cgctacgtca ttttaccgct ggcactgcgt cgtatgctgc
    35101 cgccgctggg taaccagtgg atcatcagca ttaaagacac ctcgctgttt attgttatcg
    35161 gcgtggcgga actgacccgt caggggcaag aaattattgc cggtaacttc cgcgcccttg
    35221 agatctggag cgccgtggcg gtgttctatc tgattattac cctggtgctg agctttattc
    35281 tgcgtcgtct ggaaagaagg atgaaaatcc tgtgattgaa tttaaaaacg tctccaagca
    35341 ctttggccca acccaggtgc tgcacaatat cgatttgaac attgcccagg gcgaagtcgt
    35401 ggtgattatc gggccgtccg gttccggtaa atcgaccctg ctgcgctgca tcaacaaact
    35461 ggaagaaatc acctccggcg atctgattgt cgatggcctg aaggtcaacg atccgaaagt
    35521 tgacgagcgc ctgattcgcc aggaagcagg tatggtgttc cagcagtttt acctcttccc
    35581 gcatctgacg gcgctgggaa acgtcatgtt tggtccgcta cgcgtgcgtg gcgcgaacaa
    35641 agaagaggcg gaaaaactgg cgcgtgagct gctggcgaaa gtcggtctgg cagaacgtgc
    35701 acatcactac ccttccgaac tttctggtgg tcaacagcag cgtgtggcga ttgcccgcgc
    35761 gctggcggtg aagccgaaaa tgatgctgtt tgatgaaccg acttccgctc ttgacccgga
    35821 actgcgccat gaagtgctga aggttatgca ggatctggct gaagaaggga tgacgatggt
    35881 gatcgtgacc cacgaaatcg gttttgccga gaaagtagct tcgcggctga tctttatcga
    35941 caaaggccgg attgcggaag atggcaatcc gcaggtgttg atcaagaacc cgccgagcca
    36001 gcgcttgcag gaatttttgc agcacgtctc ttaataagac acattgcctg atgcgctacg
    36061 cttatcaggc ctacaggata tctggcaact tattaaaatt gcatgaactt gtaggacgga
    36121 taaggcgttc acgccgcatc cggcaaaaaa gcccgcacgt tgtcagcaac ctgcttaata
    36181 tcccttcctc cctttcaccc gaaagggagg cacaccagat tcctctcatt taaaatcgcc
    36241 cctcccccag catctatact tatctttttg ctctgttttc tcactggagg agtcatgcgg
    36301 tggatcctgt tcatcctctt ctgcctgctg ggcgcacctg cccacgcggt atccataccc
    36361 ggcgttacaa ccacaacgac aacggactca acgactgaac cggccccgga accggatatc
    36421 gaacaaaaaa agcggcctat ggcgcactgg cggatgtgct ggataacgac acctcgcgta
    36481 aagagttgat cgaccagttg cgtactgtcg ccgcgacgcc ccctgcggaa ccggtcccga
    36541 agatcgtgcc gccgacgctg gttgaagagc aaaccgtgct gcaaaaggtc accgaagtca
    36601 gccgccatta tggtgaagcc ctttccgccc gcttcgggca actttatcgc aatatcaccg
    36661 gctccccgca taagccattt aatccacaaa ccttcagcaa tgcgctgagc catttttcaa
    36721 tgttagcggt attagtgttt ggtttttact ggctgattcg cctgtgcgca ctgccgctat
    36781 atcgcaaaat gggtcagtgg gcgaggcaaa aaaatcgtga gcgcagcaac tggttgcagc
    36841 ttccggcgat gattattggg gcgtttatta tcgacctgct gttactggcc ctgacattat
    36901 ttgtcggcca ggtattaagc gacaacctga atgcaggcag tcgcaccatc gctttccaac
    36961 aaagtttgtt tctcaacgcc tttgccctga ttgaattttt caaagccgta ctacgcctga
    37021 ttttttgccc aaacgtggcg gagctgcgcc cgttcacgat tcaggacgag agcgcccgtt
    37081 actggggtcg tcgcctgagc tggttaagca gcctgattgg ctatggcctg attgtggccg
    37141 tgccgattat ctctaatcag gtgaatgtac agataggtgc gctggcgaac gtcatcatta
    37201 tgctgtgcat gaccgtctgg gcgttgtacc tgatcttccg aaataaaaaa gagattaccc
    37261 agcatatgct caactttgcg gagcattcgc tggctttttt cagcctgttt atccgcgcct
    37321 ttgcgctggt gtggcactgg ctggcaagcg cctattttat catgctgttt ttcttttcgt
    37381 tgttcgatcc gggcaacagt ctgaaattta tgatgggtgc aacggtgcgc agcctggcga
    37441 ttattggtat cgcagcgttt gtttccggta tgttttcccg ctggctggcg aaaaccatca
    37501 ccctctcgcc acatactcag cgtaactatc cggagctaca aaaacggctg aatggctggc
    37561 tttcggcggc gctgaaaacg gcgcgtattc tgaccgtctg cgtggcagta atgttgctgt
    37621 tgagcgcatg gggattgttc gatttctgga actggctgca aaacggcgcg gggcagaaaa
    37681 ccgtagatat cctgatccgc atcgcactca ttcttttctt ctcggcggtt ggctggacgg
    37741 tgctcgccag tttgatcgaa aaccggctgg cttcggatat tcatggccgc ccgctaccca
    37801 gcgcccgtac gcgtaccctg ctgacgctgt tccgtaacgc gctggcggtg attatcagta
    37861 ccatcaccat catgattgtg ttgtcggaaa tcggcgtcaa tatcgcgcca ttgctggcag
    37921 gtgccggggc attaggtctg gctatctcgt ttggttcgca aacgctggta aaagatatta
    37981 tcaccggggt atttattcag tttgaaaacg gcatgaacac tggagatttg gtgactatcg
    38041 ggccgttgac cggcacagtg gaacggatgt cgattcgctc cgtgggcgtg cgccaggata
    38101 ccggggcgta tcacatcatt ccgtggtctt cgataaccac ctttgctaac ttcgtccgcg
    38161 gtattggttc ggtagtggca aactacgatg ttgatcgcca tgaagatgct gataaagcca
    38221 atcaggcact gaaagatgcg gtaacggaat tgatggaaaa cgaagaaatt cgcgggctga
    38281 ttattggtga accgaacttt gccgggattg tcggcttaag caataccgcg tttacactgc
    38341 gtgtttcgtt caccacgctg ccactcaaac agtggacggt acgctttgcc ctcgacagcc
    38401 aggtgaaaaa acatttcgac ctggcgggcg ttcgcgcgcc agtgcagact tatcaggtgc
    38461 tgcctgctcc gggcgcgacc ccggctgaac cgctgccgcc gggggagcca acgctttaac
    38521 gttggcgatt aacaaaacgg cggcgctgct cgtcgttcat aaaggtccag gcaataaagc
    38581 gactctgctt ttgcccctgg gccatctctt tttttaccac cttcaccgcg cccacgtcgg
    38641 tcagagcacg atacaacggc ggtaagtttt caccacgaga taccagcgag gtaaaccaca
    38701 tcacctgctt cgcgaagcct ttgctctctt caatcatctt tttgataaag ttgacttcac
    38761 cgccttcaca ccacaactcc tgttgctgac caccaaagtt cagtgcatcg tctttgttca
    38821 gccccaggtt acggcgttta cgctcactac ctgcccgcgc cgcagcggcg gaatcgtgga
    38881 atggcgggtt acacaaggtc gcgtcgtatt gctcgttttt gtggatgatg ccgttaaaaa
    38941 tcgccccact ctctttttgc cgacgcagac gaatggcgcg gttaagcccc ggattagcac
    39001 tgataatcgc ctgcgcactg cttaatggtt tcactaccgg taaaacgcca gccatattca
    39061 tgtacgccaa tcagcggata aatacagttc gcaccaacgc cgatatccag aatgctggca
    39121 ttcgctggaa ttgttccgct tgcttcagcc agtaaatcgg caaggtgatg aatataatcc
    39181 gcccggcccg gtactggtgg gcagagaaaa ccgtcgggga tatcccagtt cgctacggcg
    39241 taaaaatggg ccagcaacgc cttattgagc gccttcaccg ccagcggatt ggcaaagtct
    39301 acgctttgct cccctgcggg tgtaagcgtg aggaattgcc tgagttcagg attgacctga
    39361 caaagcgtgg cgagatcgta gcggctgtga tgacggttgc gcggatgcaa ccccggtttc
    39421 tgggcggaca tagcattctc ctttaagcag cggcgtaaga tacccgcata acgcgacagg
    39481 gtaaatatct ggctgcgttt atcattcccc aaaaccttaa catggctatg gtttatggca
    39541 attcatcata aaaatcagca ttatgcatgt cattgatttg tcttattttg taaaaatata
    39601 tcgttcaaaa aataagcatt tctctgtcaa ttttttgcgt catttgcatc gcgcaggcga
    39661 cctacactta tcaggcacta cccgttgtat ttatgaggat gtcattatga aaaagtgcct
    39721 cacactactg attgccaccg tcctgagcgg aatctctctc acggcttatg ccgcgcaacc
    39781 gatgagtaac ctggacagcg gtcagctacg gcccgccggt accgtttcgg caactggcgc
    39841 atcaaaccta agcgatctgg aggataaact ggcagaaaaa gcgcgcgaac aaggcgcgaa
    39901 aggttatgtc attaattccg caggcggaaa tgaccagatg ttaggtactg caaccatcta
    39961 caaataaccc caccaacgta actgcattcg cctctggatg cagttttcgc ctgttttgta
    40021 atcaaaaaac atcaaatgtg aacgcaatgt aaataaatgt atttcttttt cgcgcaatgg
    40081 gtgatagaaa atcgcatcaa gtgataatgc ttatcaaaat tattatcact tgcacgaaca
    40141 ctatcacggg attaacagtg gcatcgcatc cgcagagatg ctttctcgtg gcagtgaaaa
    40201 tttcaacata taagaaaaag tcacctgcaa aatggaaaac aatcgcaatt tccctgccag
    40261 acaatttcat tcgctcacgt tctttgccgg tctttgtatt ggtattacgc ctgtggcaca
    40321 ggcactcgcc gccgaagggc aagctaacgc ggatgacacg ctggttgtcg aagcatcaac
    40381 gccttcgctt tatgcgccac aacaatctgc cgatccgaaa ttctcgcgtc cggtagcgga
    40441 tactacccgc acgatgacgg tgatttctga acaagtgatt aaagatcagg gcgcaaccaa
    40501 ccttaccgac gcgctcaaaa acgtccccgg cg
//
LOCUS       DAJTSA010000028        39987 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000028 DAJTSA010000000
VERSION     DAJTSA010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39987
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.028"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..155
                     /locus_tag="OO369_001587"
     CDS             <1..155
                     /locus_tag="OO369_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5981905.1"
                     /translation="EQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG
                     GVQELN"
     gene            384..974
                     /gene="uidR"
                     /locus_tag="OO369_001588"
     CDS             384..974
                     /gene="uidR"
                     /locus_tag="OO369_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR5981906.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1362..3065
                     /gene="uidA"
                     /locus_tag="OO369_001589"
     CDS             1362..3065
                     /gene="uidA"
                     /locus_tag="OO369_001589"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR5981907.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3071..4096
                     /gene="uidB"
                     /locus_tag="OO369_001590"
                     /pseudo
     CDS             3071..4096
                     /gene="uidB"
                     /locus_tag="OO369_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4135..5400
                     /gene="uidC"
                     /locus_tag="OO369_001591"
     CDS             4135..5400
                     /gene="uidC"
                     /locus_tag="OO369_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR5981908.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5626..7134)
                     /locus_tag="OO369_001592"
     CDS             complement(5626..7134)
                     /locus_tag="OO369_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5981909.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7235..8410)
                     /gene="manA"
                     /locus_tag="OO369_001593"
     CDS             complement(7235..8410)
                     /gene="manA"
                     /locus_tag="OO369_001593"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR5981910.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8609..10255
                     /gene="fumB"
                     /locus_tag="OO369_001594"
     CDS             8609..10255
                     /gene="fumB"
                     /locus_tag="OO369_001594"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5981911.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10398..11801
                     /gene="fumC"
                     /locus_tag="OO369_001595"
     CDS             10398..11801
                     /gene="fumC"
                     /locus_tag="OO369_001595"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR5981912.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11798..12727)
                     /gene="tus"
                     /locus_tag="OO369_001596"
     CDS             complement(11798..12727)
                     /gene="tus"
                     /locus_tag="OO369_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR5981913.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="OO369_001597"
     CDS             complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="OO369_001597"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR5981914.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14283..15602)
                     /locus_tag="OO369_001598"
     CDS             complement(14283..15602)
                     /locus_tag="OO369_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCR5981915.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="OO369_001599"
     CDS             complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="OO369_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR5981916.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16699..17034
                     /locus_tag="OO369_001600"
     CDS             16699..17034
                     /locus_tag="OO369_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR5981917.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17031..17753)
                     /gene="folM"
                     /locus_tag="OO369_001601"
     CDS             complement(17031..17753)
                     /gene="folM"
                     /locus_tag="OO369_001601"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR5981918.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17790..19172)
                     /locus_tag="OO369_001602"
     CDS             complement(17790..19172)
                     /locus_tag="OO369_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5981919.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19358..20302)
                     /locus_tag="OO369_001603"
     CDS             complement(19358..20302)
                     /locus_tag="OO369_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5981920.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20826..22358
                     /gene="pntA"
                     /locus_tag="OO369_001604"
     CDS             20826..22358
                     /gene="pntA"
                     /locus_tag="OO369_001604"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR5981921.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22369..23757
                     /gene="pntB"
                     /locus_tag="OO369_001605"
     CDS             22369..23757
                     /gene="pntB"
                     /locus_tag="OO369_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR5981922.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="OO369_001606"
     CDS             complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="OO369_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR5981923.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25227..25592
                     /gene="mdtJ"
                     /locus_tag="OO369_001607"
     CDS             25227..25592
                     /gene="mdtJ"
                     /locus_tag="OO369_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR5981924.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25579..25908
                     /gene="mdtI"
                     /locus_tag="OO369_001608"
     CDS             25579..25908
                     /gene="mdtI"
                     /locus_tag="OO369_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR5981925.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25947..26768)
                     /locus_tag="OO369_001609"
     CDS             complement(25947..26768)
                     /locus_tag="OO369_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR5981926.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26868..26951)
                     /locus_tag="OO369_001610"
     CDS             complement(26868..26951)
                     /locus_tag="OO369_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981927.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27044..27379)
                     /locus_tag="OO369_001611"
     CDS             complement(27044..27379)
                     /locus_tag="OO369_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR5981928.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27776..29029)
                     /locus_tag="OO369_001612"
                     /pseudo
     CDS             complement(27776..29029)
                     /locus_tag="OO369_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29136..30047
                     /locus_tag="OO369_001613"
     CDS             29136..30047
                     /locus_tag="OO369_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5981929.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAKEPFVFFDPHVGTGLYDDILGL
                     MRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSE
                     MWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            30182..31402
                     /gene="mlc"
                     /locus_tag="OO369_001614"
                     /gene_synonym="dgsA"
     CDS             30182..31402
                     /gene="mlc"
                     /locus_tag="OO369_001614"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR5981930.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31527..32222
                     /gene="bioD"
                     /locus_tag="OO369_001615"
     CDS             31527..32222
                     /gene="bioD"
                     /locus_tag="OO369_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5981931.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32175..33467)
                     /gene="clcB"
                     /locus_tag="OO369_001616"
     CDS             complement(32175..33467)
                     /gene="clcB"
                     /locus_tag="OO369_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR5981932.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33626..34240)
                     /gene="dmsD"
                     /locus_tag="OO369_001617"
     CDS             complement(33626..34240)
                     /gene="dmsD"
                     /locus_tag="OO369_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR5981933.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34283..35137)
                     /locus_tag="OO369_001618"
     CDS             complement(34283..35137)
                     /locus_tag="OO369_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5981934.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35139..35756)
                     /gene="dmsB"
                     /locus_tag="OO369_001619"
     CDS             complement(35139..35756)
                     /gene="dmsB"
                     /locus_tag="OO369_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5981935.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35767..37805)
                     /locus_tag="OO369_001620"
                     /pseudo
     CDS             complement(35767..37805)
                     /locus_tag="OO369_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37866..38975)
                     /locus_tag="OO369_001621"
     CDS             complement(37866..38975)
                     /locus_tag="OO369_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5981936.1"
                     /translation="MINVSHIRTLSSIESTTSSPSIRLAMESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLEDLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            38958..>39987
                     /locus_tag="OO369_001622"
     CDS             38958..>39987
                     /locus_tag="OO369_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5981937.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYRYQQVYTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNLTDDSTIGDSTSILNNEQGDRPAVFTQE
                     RQSSVVFSSLVSSLLAEKVQTLKTSSYKDLSSSLNTVSRFLPEDMDLMSRSGWLAVRD
                     SMLASEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDIDSKRRAFTDEE
                     LERLLVRVE"
BASE COUNT        10163 a         9701 c        10242 g         9881 t
ORIGIN      
        1 ttgaacaaaa aatgttacag cacacgccga tcagacgtct gggccaaccg caagatattg
       61 ctaacgcggc gctgttcctt tgctcgcctg cagccagctg ggtaagcgga caaattctca
      121 ccgtctccgg tggtggggta caggagctca attaatacac taacgaaccg gtaaacggtc
      181 gtatgtgttg tttaccggga tagcgtgtgc aattcatcaa cgtctctgct aaacaactgg
      241 cagccaaatc acggctattg gttaaccaat tgctgggtga aaagtatacg aatagagtgt
      301 accttcgcac tattcaacag caatgatagg cgctcacctg acaacgcggt aaactagtta
      361 ttcacgctaa ctataatggt ttaatgatgg ataacatgca gactgaagca caaccgacac
      421 ggacccggat cctcaatgct gccagagaga ttttttcaga aaatggattt cacagtgcct
      481 cgatgaaagc catctgtaaa tcttgcgcca ttagtcctgg gacgctctat caccatttca
      541 tctccaaaga agccttgatt caggcgatta tcttacagga ccaggagagg gcgctggctc
      601 gtttccggga accgattgaa gggattcatt ttgttgacta tatggtcgag tctattgtct
      661 ctctcaccca tgaagccttt gggcaacggg cgctggtggt cgaaattatg gcggaaggga
      721 tgcgtaaccc acaggtcgcc gccatgctta aaaataagca tatgacgatc actgaatttg
      781 ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat aagcccagac atcaacacgg
      841 caatgacttc acgtttactg ctggatctga cctacggtgt actggccgat atcgaagcgg
      901 aagacctggc gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt ggcggtatat
      961 taaccgcatc ctgatcctct catttcgcgg gctggtgata actgtgcccg cgtttcatat
     1021 cgtaatttct ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc cccaaaatat
     1081 tcaccgtagc catatgtcat gagagtttat cgttcccaac actctcgaac gaacgttcga
     1141 ttgcttattt tatggcttct gtcaacgctg ttttaaagat taatgcgatc catatcacgc
     1201 tgtgggtatt gcagtttttg gttttttgat cgcggtgtca gttcttttta tttccatttc
     1261 tcttccatgg gtttctcaca gataactgtg tgcaacacag aattggttaa ctaatcagat
     1321 taaaggttga ccagtatttt tatcttaatg aggagtccct tatgttacgt cctgtagaaa
     1381 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     1441 actgtggaat tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg gcaattgcag
     1501 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gtgggcaacg
     1561 tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc
     1621 gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc
     1681 atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa
     1741 gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa
     1801 tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact
     1861 atgccgggat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata
     1921 tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg
     1981 tggccaatgg tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg
     2041 gacaaggcac cagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag
     2101 gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc
     2161 tgcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac
     2221 cgttctactt tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc aaaggattcg
     2281 ataacgtgct gatggtgcac gatcacgcat taatggactg gattggggcc aactcctacc
     2341 gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg
     2401 tggtgattga tgaaactgca gctgtcggct ttaacctctc tttaggcatt ggtttcgaag
     2461 cgggcaacaa gccgaaagaa ctgtacagcg aagaggctgt caacggggaa actcagcagg
     2521 cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga
     2581 tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc
     2641 cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa
     2701 tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc
     2761 gttattacgg ttggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
     2821 aagaactgct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
     2881 tggatacgtt agccggcctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
     2941 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
     3001 aggtatggaa tttcgccgat tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca
     3061 attgacctac atgggcctcg ggctttgcta cagcctggtg aatattcctt atggttcact
     3121 tgctaccgcg atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg ctcgtgggat
     3181 tgccgcttca ttgacctttg tatgcctggc atttctgata ggaccgagca ttaagaactc
     3241 cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg tgattgccgg
     3301 aatggtgctt tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac gtatcgtggc
     3361 gcagccgtca ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc tgtttatgtt
     3421 gtgcatcggt gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt
     3481 ctacgtgcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa
     3541 cctggttggt actgtggcat cggcaccgct tgtgccgggg atggtcgcga ggatcggtaa
     3601 aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg
     3661 ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt caattggtca
     3721 gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagaat acggtgaata
     3781 cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg
     3841 cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc
     3901 caatcaggtg taaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc
     3961 ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa
     4021 attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag
     4081 cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga
     4141 aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttac
     4201 gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc
     4261 agggctgata agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga
     4321 ttgctcgatt tcaatagtgg ttattattcc aatattgttg gcgttgaagg cggagcgtat
     4381 tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat
     4441 aaaagttttg gctttgccct gggggcagta aaaataaaac ccagtgaaaa tagcctgctt
     4501 aaattaggtc gcttcgggac ggattatagt tatggtagct taccttatcg tattccgtta
     4561 atggttggca gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg
     4621 gctttaacgc caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact
     4681 gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat
     4741 aaacatcgcg cacgttcttt tttagccgcc agttggtatg atgataccag tcgctattct
     4801 ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc
     4861 ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc
     4921 gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg
     4981 acctggaatg cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg
     5041 aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac
     5101 cgtgaaggaa tgcagtcctg gcaattaggt gccaactacc gtgtaactcc gcaatttacg
     5161 ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt
     5221 gaaggcgcgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc gttacaaggg
     5281 atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc
     5341 gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa
     5401 gaaataacgc cggagagaaa aacctccggc gtttcagatt gttgacaaag tgcgcgtttt
     5461 ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagtg
     5521 ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca
     5581 gatgaaaacg ccggagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat
     5641 tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa
     5701 cgacattttc tgcccattta acgttatctg accgttagca tattgcaggc tggtggtgat
     5761 ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac
     5821 ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg
     5881 agtcatcaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac
     5941 cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt
     6001 caggaacagc gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc
     6061 gatagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga aggcttccgt
     6121 cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg
     6181 agtttgcgcg ttatattgct ggctaaactg atgccaggct tcaccatcaa tctggccgac
     6241 ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga
     6301 gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt taccgctgat
     6361 ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag
     6421 tgacagtttt tggtttccta cgcgctcacc aaaacttccc agcgtgctgg aaccgtcggt
     6481 tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg
     6541 accactttgc gcctctccgg aaagggagat ggctttgccg tctctgtcag catttaactg
     6601 gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggattgag
     6661 cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt
     6721 ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg
     6781 aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc
     6841 atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt
     6901 cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc gatgatagtt
     6961 ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg cgttcgcctg
     7021 cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt
     7081 ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat
     7141 tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag
     7201 cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta
     7261 aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac
     7321 ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga
     7381 acaaaatggc ggcactctgc tggctgatgg tggtttcttt atcactaagg tcatgcagcg
     7441 agaaggcaaa atcatccaca ggaatcggga agtccagttc tgcaccttgt ttcaccggct
     7501 gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa
     7561 tatcaatgta tttaggcgtc agacctgcac gcagcacgtt atcggagttc gccatcactt
     7621 ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc
     7681 caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt
     7741 aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg
     7801 attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac aggttggcga
     7861 acagttcgct taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc gccggatgtg
     7921 caccggcgac cggctggagt agggagacaa tctcggaaaa ttcacgaaac gcgttcatcg
     7981 caaggaaagg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac
     8041 gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat
     8101 tgtgtttgtt tggatgaacc tgaatggaga gtggctgtgc tgcgcataat actttgaaca
     8161 ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac
     8221 tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc
     8281 ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac
     8341 cataaagttc agtcaacgcc gttttgctac cccaggcata gttttgcact gagttaatga
     8401 gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccgctatt aaagcaagaa
     8461 tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa
     8521 actgtgtagg atattgttac tcgcttttaa cagggcaacg gaacacccgc ccagagcata
     8581 accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcagtctcc
     8641 ttttccactc aaaaaaaatg atactgagta ttacctgcta accagtgaac acgttagcgt
     8701 atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc
     8761 gcgtcaggcg tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac aacaggtggc
     8821 cgacattctg cgtgacccgg aggccagcga aaatgataaa tatgtggcgc tgcaattcct
     8881 gcgtaactcc gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata ccggcaccgc
     8941 gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg aagcggcgct
     9001 ggcgcgcggt gtctataaca cttatatcga agataatctg cgctactcgc aaaacgcgcc
     9061 gctggatatg tataaagaag tgaataccgg caccaatctg ccagcgcaga tcgatcttta
     9121 tgccgttgat ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg gttcggcaaa
     9181 caagacgtat ctctatcagg aaaccaaagc gttactgacg ccggggaaac tgaaaaatta
     9241 cctggttgag aagatgcgca cgctgggtac cgcggcctgt cctccgtatc atattgcgtt
     9301 cgttattggt ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg cttccgcgaa
     9361 atactatgat gaactgccaa cggaagggaa tgagcacggt caggcgttcc gcgatgtgga
     9421 actggaaaaa gaattactga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg
     9481 taaatacttc gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg catcctgtcc
     9541 ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca
     9601 ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg
     9661 taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct
     9721 cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt
     9781 tggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc
     9841 gcaatacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta
     9901 tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact
     9961 gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac
    10021 ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt
    10081 attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga
    10141 agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg
    10201 aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga
    10261 gccgcccttc ggggcggttt ttttacatgg cacgaaagac caaacatttg ttatcaaata
    10321 gtaaataatg agtgagctaa aagttgctta acgaaagcaa aacagaaaga aaaaattaat
    10381 caggtgagga gcaggccatg aatacagtac gcagcgaaaa agattcgatg ggggcgattg
    10441 atgtcccggc agataagctg tggggcgcac aaactcaacg ctcgctggag catttccgca
    10501 tttcgacgga gaaaatgccc acctcactga ttcatgcgct ggcgctaacc aagcgcgcag
    10561 cggcaaaagt taatgaagat ttagacttgt tgtctgaaga gaaagcgagc gccattcggc
    10621 aggcggcgga tgaagtactg gcaggacagc atgacgacga attcccgctg gctatctggc
    10681 agaccggctc cggcacgcaa agtaacatga acatgaacga agtgctggct aaccgggcca
    10741 gtgaattact cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac gacgacgtga
    10801 acaaaagcca aagttccaac gatgtctttc cgacggcgat gcacgttgcg gcgctgctgg
    10861 cactgcgcaa gcaactcatt ccgcagctta aaaccctgac acagacgctg agtgaaaaat
    10921 cgcgtgcatt tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac gccacgccgc
    10981 taacactagg gcaggagatt tccggctggg tagcgatgct cgagcataat ctcaaacata
    11041 tcgaatacag cctgcctcac gtagcggaac tggctcttgg cggtacagcg gtgggtactg
    11101 gactaaatac ccatccggag tatgcgcgtc gcgtagcaga tgaactggca gtcattacct
    11161 gtgcaccgtt tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt gatgccctgg
    11221 ttcaggcgca cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc gccaatgatg
    11281 tccgctggct ggcctctggc ccgcgctgcg gaattggtga aatctcaatc ccggaaaatg
    11341 agccgggcag ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa gcattaacca
    11401 tgctctgctg tcaggtaatg gggaacgacg tggcgatcaa catgggtggc gcttccggta
    11461 actttgaact gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa tcggtgcgct
    11521 tgctggcaga tggcatggaa agttttaaca aacactgcgc agtgggtatt gaaccgaatc
    11581 gtgagcgaat caatcaatta ctcaatgaat cgctgatgct ggtgactgcg cttaacaccc
    11641 acattggtta tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa gggctgacct
    11701 taaaagctgc ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac agctgggtac
    11761 ggccagaaca gatggtcggc agtatgaaag ccgggcgtta atctgcaaca tacaggtgca
    11821 gccgtggaat gatcaaacga agcggctgcg cctgaggttt ataacggtgc tgcacattgt
    11881 cggcatcgta atttaacaac tcaccaacgt ccggcacgcc cgcgccatta tcccgattaa
    11941 tcagtgcaat cagtggtgta gggcaggcgt gttggacttg tttttgatct cctttgtacc
    12001 agacgcgggc aatcggctgc accttcactg gacgtttgat ttttaacttc gcgttctgtg
    12061 gcagggcagc gatatcctga tactctcgct ccagttttct ttgccactcc tcgcgcgtcc
    12121 acggtgcgac actgcgtggt gatttcaggc ttttttccag ctgtgccagg acttcatcac
    12181 gatgcaaatt cttaatgata tgtttattag cccaaccaaa gcgtaaagtg gcggggtcgt
    12241 gcagaacggt gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa tgacgatgca
    12301 cccattcaaa acgtgccgcg gtggggagtt ctgattcaac cgtgacgata tgctcgaacg
    12361 tggtcttgag tttattgatg tgctgaatat gactgaccaa cgctgcttgc gaaaggttat
    12421 cgacctggta acacaacacg ccaggcagac ggacagcggc cttgctgctg cgattttcgg
    12481 actgttgttg aataaataaa tggcggaaat gacgcaacgc cagcgactgc gcgtcgttgc
    12541 cgagatgttg ttttacctca atacgattaa gcggattatg ctcatcctct ttttttacct
    12601 ccggcaaaga gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga gcggcaaatg
    12661 tagccagctc ttgttccatc tggcgaaagg tagtgttgag tcggtctacg agatcgtaac
    12721 gcgccataat attaaccact ttagttacaa catacttatt ttatagcaca gtcgtggtga
    12781 cttgaccatc gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta tgccataacg
    12841 gccagctaaa cgagaagcga gcaccaccca gttcgctgat gtcacaatta accgtaccgc
    12901 ccattgtcag tgctattgag tggacaattg ccagccccag cccgcagccg ccggttgagc
    12961 gatcccggct gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg ttttctgggg
    13021 caatccctgg gccatcatcc tcaacaatta atgtcgctct attccccgac agtagcaggc
    13081 tggtttcaac cgttgaatgg cagtagcgca gggcgttatt gagcaaatta tccagcacgc
    13141 gctccattaa gcgcatatcc aacgccgcat aatggccttg catgagcgtt tttatccgta
    13201 ccgttttatc gggcgttact gcctgaatat ctgccagatg cgttgacagc cacaacggca
    13261 ggtctggttc gctaagatga agctcgtttt gtgggcgatc gagtcgggca taagtcagca
    13321 gctcttcaat taaagcttca agttgactga tatcacgatt caacgcctgg gattcggcgg
    13381 cgctcaggtt atcgctcatc tccagtcgat aacgcaggcg cactaacggt gttcgcagtt
    13441 cgtgagcgat accgtcaata agctgttttt tgctggcaat taaggcgttg atattgtccg
    13501 ccatctggtt aaatgcgata ccaagtcgtt caaagctcga accctcatca aagtggatac
    13561 gttcattgag atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt aacatatcct
    13621 gccagtgcgg acgcatccag ataaacaccg gaaaggcgag ggaaatagca ataaaagcga
    13681 tcagggcgat atccagcaat cgcatttgat ggaggtaata aagataagga acaggaccaa
    13741 ctgccagcac gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga tcgtccaggg
    13801 cgacaatttc gccgccacgc agtcggtgca tggaaatatc atcaagatgg tatttactca
    13861 gtggctcgac acgcagatcg aaagagagat ttaaatccat ctctttcagc gtcttacccc
    13921 agtcgtgtgg ggggatctct cgcaattcgc tgcgcatcag atacagcgaa ctgttcatca
    13981 aatcatccag cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc agcccaacca
    14041 gcagagacat cacaaggaag cagacaaaca ataacaggta aaactggata aacaattttt
    14101 tcattggcat cgcccgcaac agaatactat tcgtattaat tcacgactgt ggagcaatgt
    14161 aaaccgccaa ctggataaga ccttgtttgg aagataatgt ccggaacaga cggatgtatc
    14221 cccggtgtat tcatattcag taaattcaat tttccaccgg ggcttgttta ttatgctaag
    14281 gtttaattta acgtgacgct ttgttgttct ttagtctctc tatccttaag aagacgggct
    14341 gatattacac cggacattat tgaaccattc acattaagcg ctgttcttgc catatcaacg
    14401 atcggctcta tggcaataaa aataactact aatgcgacag ggaaattaag tgttgaaagg
    14461 acaatcagcg ccgcgaatgt cccaccacta cctacgcctg ctacccctat tgagccaagc
    14521 gcgatggcag gtaaaagagt tgcgaggaat tgcaatgaga agggatcgat acccattgtt
    14581 ggagcaatca tcgccaccat cacggcagga taaatcccag cgcagccatt ttgtcccatg
    14641 ctggaaccaa aagacgctgc aatattagca atcgtatttg gaacccccaa tttttccaat
    14701 gcagaaatag ccagaggaat tgaagccgca ctactgcgag aaacaaacgc aaaggtaagt
    14761 acttgccaga cggtcttgta atagttaacg gggtttttac ctattaacag caaaatgccc
    14821 gagtgaacaa taaacatcac aaaaacggcc agataacagg cgacgatgaa cccgagcaaa
    14881 ttggtaaact gtgaaagctg atacgttgag aagactttcg ccattagcgc cattacccca
    14941 taaggcgtca gcgcaatgac aatacggacc attttcatta cccatatttg aatcgcgttg
    15001 atccccgcgc tcaatttttc gccctctgct ggtgcatctt tctttacttt taacagcgca
    15061 ataccagcaa gaattgtaaa gatgacgatg ccaataaccg aaacacttct tgccccggtc
    15121 agatcaagga agaggttggt tgggatcaat gacgtaatga gtcgcggtaa agataccgca
    15181 gctgttttcg agacgtcatc gctggttaaa gaggattgca tatgaccaaa ggcgctggca
    15241 tcaaggccaa ttgcactggc aatagccagg ccaactaaac ctgcaatcat gactaaacac
    15301 aacatacagg caacaatagt taaagacatc ttgccaatac cagcgctatt ttcgagctta
    15361 ttaatcgctg aaagaatcga aatgaatatg agtggaacgg caacaagttt taataaatta
    15421 acataaccgt taccagcaat attataccat tcgataacgg tttcactgct tgtctcagga
    15481 atgaaatgca gggagatacc aatgatagcc cccaggaaaa tcgcgataaa aacacttttg
    15541 gaaagggatt cgctcttttg atccgttttg aaaatccccc atgagcagat caaaaaaagc
    15601 acaatatatg tgaaagttgt aaccgttttt taagcctctt gcctcattca gcacctgaaa
    15661 tgccggaaag agtttgttgt aaaaaatctg cgccataaga cgcagggacg aggcgattat
    15721 agagaaattt tttctcattt tcatatcaaa tgtagggggt ttggaatttg aataagctct
    15781 tattaattga attcgttaat tgttctcttt ttcagatgaa caaatattag tcatctgatt
    15841 gtttattgtc actatcccat gcatgaggcg caaaaagata gcctttgtta cgcacagttt
    15901 taatgcgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc gaaatagcca
    15961 cgtccacgct acgatccagt ccgtcataac tgacgccgcg taaatttttc agcaatgcat
    16021 cgcggtccat gatttgcccg gcatgcgtag ctagttccca caataattcg aaatcagctg
    16081 tcgagagcga gatttcagtg ttagccaggg tgactacgcg gttgatgggg tcgatagtta
    16141 acgtgccgaa atgcagggct ttgtagggag taagagacgt ttcctgaaga cctttggtca
    16201 gtgtggcttg ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa acagcagggg
    16261 gcgtcgtttt gagaatatag tcgcaggcac ccatttccag tgccaggatg tggttcatat
    16321 cgctatcgag agaggttaga agaacaatcg gtccagacca ctttgcgcgt aaatcacgac
    16381 aaatggtcat gccgtccttg cctggtagca tgatgtcgag taacaccaaa tccggatttt
    16441 ctcgcaaaat ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc tgcatatcat
    16501 gttttgccag gtacgcggca atcagtgaac cgacttccgc atcatcttcc acaaatacga
    16561 tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgcg ctgtttttac
    16621 ttactatcaa tcgtttctaa acaagttttc atcaggtatt ctactgatga cttgttatgg
    16681 aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc gctggtagtg
    16741 ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct gattccactt
    16801 ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgcgg cattgaagcc
    16861 ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt ttacctggtg
    16921 tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg ttctgttgcg
    16981 tgttggggga taagcgcatg ggtgttgatt atatgctgga taaagctgca ttaacgcaga
    17041 tgacgaccgc catcaagtgg gaaactgcgt ccggtgacaa agcaactggt aagtaagtaa
    17101 tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga tttattcagc
    17161 gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga tggcgcaata
    17221 gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat attatccagt
    17281 gccgctttgc ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac cacataatcg
    17341 gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct ttccagcgca
    17401 tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag tacgtcggcc
    17461 agtggcgcac ccggtttttc cgcaatccac gcactggcgt tatgcaaaat agcacgcaga
    17521 ccatgggtgg tttttagtac ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa
    17581 tcagcctgaa tacactgcgc acctgcatta atcagtccat caatggctgg atagtgtgtc
    17641 cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccatgcgag ggcgaggccg
    17701 atgcgacgac ctccgccagt aattaatatt ggcaagggct gggttttacc catcgttatc
    17761 tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt cgccggaact
    17821 gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac attatcatgt
    17881 gtatgcgttt tgcgcgcata caggaaaacc agcagccccg gtgcatacag aacaacggat
    17941 aacagcaagt gcattggacc agaggcgtac aataaccata agccataaat gcaggcaccg
    18001 acacctaccg ctttatgcag tggacgggtt gcgattttca gcaagaacgc gccgaccagg
    18061 aaatagggca ccagaatcat ttctgaggcg atggtcagca acgtgttgta atcggaaccg
    18121 gtcagccaga tgagtaccag acaaatttgc acgcagatgt tggtcaacca cagtgaggcg
    18181 gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt atgagtggca
    18241 gccaggaacg gaacttctgc cgccatgatg gtccagctta agtacgcgcc gcaaacggaa
    18301 acgatcaaac cggcagcgat gatgatttcg ccccatggtc ccatcatttc caccatcaga
    18361 ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac acccagcgaa
    18421 agcagcgtta ccagcaagta aacgcccaga gcggagagaa ccgccagcag tgtcgctttg
    18481 ccaacatcac gtttattacg cgcacgcgca gaaacaacca cggcaccttc cacaccaatg
    18541 aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccaaacagg tacgccaagt
    18601 gcaagtccgg tgaagtcgag cttgaaggta tccagtttga acatcatcat cgccagcaca
    18661 acaaacagac ccagcggcaa caattttgcc agtgtcgcca ccaggttaat gctggcagcg
    18721 gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga tgcaccgact
    18781 atcgattgcc aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc cgtaaaaaag
    18841 cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgaccgc gcacagccag
    18901 tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc ataggtaaag
    18961 ataccgccat caagttcggg acgaatgcgc gtgaggatca gcatggcaaa ggccagcaat
    19021 aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct ggcaactgcc
    19081 gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg
    19141 gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa acgtgataag
    19201 aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag cgcgggattt
    19261 tacgaagaga tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa aaatgcgaaa
    19321 ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga tctgcgctga
    19381 cggtcaggtt attaccacgt ttctgccact ggcgggtgat gtggtaatac ttagcacctt
    19441 tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg tagttgccag
    19501 agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc agttcttcga
    19561 ctttagtgcc atccgggagc gtgacggtgt aacgcccgcc ttttgatgac tgggtttcaa
    19621 agaagcgacc gacttcagaa cttggtgatg cggtagtcgc aacacccggg atctcaactt
    19681 tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa tctgccggga
    19741 tcacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg aatgcagtaa
    19801 tacgctggtt gccgccctgg ttggcatcga tttgacgaac gatgtagaaa gagtaggcac
    19861 ctttcgcttt tgccgctttg gtgatggcat cattgacttc tggctggcta cgatagaagc
    19921 cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc ggcagttcga
    19981 caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg gctttataga
    20041 ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg acaacataaa
    20101 aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct tcgccaatag
    20161 cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc gcttgctccg
    20221 gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt gccgacgcca
    20281 ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc aggtactggt
    20341 attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc
    20401 atttttcata tcacattcct taaaccaatt ttaatcctgc tcaaatgacc gtctatgctt
    20461 aaaaaacagc cgtatcagca tcattgctac tgaagcaact gaattgtata agttaattta
    20521 atgttaagta gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt cgaagacaat
    20581 tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg aatcgttaat
    20641 attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc ataattttca
    20701 gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta gctcgtacat
    20761 gagcagcttg tgtggctcct gacacaggca aaccatcatc aataaaaccg atggaaggga
    20821 atatcatgcg aattggcata ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa
    20881 cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta gagagcggcg
    20941 cgggtcaact ggcaagtttt gacgataaag cgtttgtgca agcgggcgct gaaattgtag
    21001 aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg ttagatgatg
    21061 aaattgcgtt actgaatccc gggacaacgc tggtgagttt tatctggcct gcgcagaatc
    21121 cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg gactctgtgc
    21181 cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaat atcgccggtt
    21241 atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg caaatcactg
    21301 cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgccggtgtt gcaggtttgg
    21361 ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac acccgcccgg
    21421 aagtgaaaga acaagttcaa agtatgggcg cggaattcct cgagctggat tttaaagagg
    21481 aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa
    21541 tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc
    21601 caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg aaggcgggca
    21661 gtgtgattgt cgacctggca gcccaaaacg gtggcaactg tgaatacatc gtgccgggtg
    21721 aaatcttcag tacggaaaat ggtgtcaaag tgattggtta taccgatctt ccgggccgtc
    21781 tgccgacgca atcctcacag ctttacggta ctaacctcgt taatctgctg aaactgttgt
    21841 gcaaagagaa agacggcaat atcactgttg attttgatga tgtggtgatt cgcggcgtga
    21901 ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta tcagctcagc
    21961 cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac
    22021 cgtggcgtaa atacgcgttg atggcgctgg caatcatcct gttcggttgg ctgtcaagcg
    22081 ttgcgccgaa agagtttctt gggcacttca ccgttttcgc gctggcctgc gttgtcggtt
    22141 attacgtggt gtggaatgta tcgcacgcgc tgcatacacc gttgatgtcg gttaccaacg
    22201 cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccaggga ggctgggtta
    22261 gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt ggcttcaccg
    22321 tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat gtctggagga
    22381 ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct ggccggtctt
    22441 tcgaaacatg aaacgtctcg ccagggtaac aacttcggta tcgccgggat ggcgattgcg
    22501 ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt gctggcgatg
    22561 gtcattggtg gggcaattgg tatccggctg gcgaagaaag ttgaaatgac cgaaatgcca
    22621 gaactggtgg cgatcctgca cagcttcgtg ggtctggcgg cagtgctggt tggctttaac
    22681 agttatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca cctgacggaa
    22741 gtgttcctcg gtatcttcat cggggcggta acgttcacgg gctcggtggt ggcgttcggc
    22801 aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac
    22861 ctggcggctc tggtcgtttc cttcttgctg ctgattgtat ttgttcgcac ggacagcgtc
    22921 ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt cggctggcat
    22981 ttagtcgcct ccatcggtgg tgcagatatg ccggtggtgg tgtcgatgct gaactcgtac
    23041 tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct gattgtgacc
    23101 ggtgcgctgg tcggttcttc gggggctatc ctttcttaca ttatgtgtaa ggcgatgaac
    23161 cgttccttta tcagcgttat tgcgggtggt ttcggcaccg acggctcttc tactggcgat
    23221 gatcaggaag tgggtgagca ccgcgaaatc accgcagaag agacagcgga actgctgaaa
    23281 aactcccatt cagtgatcat tactccgggg tacggcatgg cagtcgcgca ggcgcaatat
    23341 cctgtcgctg aaattaccga gaaactccgc gctcgtggta ttaatgtgcg tttcggtatc
    23401 cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga agcaaaagta
    23461 ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga taccgatacc
    23521 gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga tccgaagagt
    23581 ccgattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa
    23641 cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc
    23701 cacatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc
    23761 tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa cggcgatgct
    23821 ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgtcaacg gcacggaaag
    23881 cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa
    23941 tgtcgaaagc cccagcccac gtcccatgat gcgcggctcc agaatattgc cgaagaccag
    24001 attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat taaacaccag
    24061 tacctgagcg atagggggga ttgccgcgag aactgaacca atattaggga tgtaattaag
    24121 cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc
    24181 ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat aatgagaaac
    24241 actgtcaatc gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca tcatttgctg
    24301 aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca gcagcaataa
    24361 aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca acgtcatcgc
    24421 cgcgttcgga tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc ctacgcgttg
    24481 caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt gcggtaacgt
    24541 ccgcgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca tcgccatcac
    24601 gatgattgtc atcaaaatcg acactgccag tacacgcggc acacgccagc ggaccatatg
    24661 ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg gcacgatgat
    24721 ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca taatgacgat
    24781 ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccgttt ttcttccttt
    24841 gctaataata atgtcttttt cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa
    24901 gaagtgaaaa tattttgagt taattcttaa gctatgatac aaatcaggcg tgttcaacta
    24961 ccgaggacaa ttatcatctg cgatgacgag aagcaacact gcggataatt gtaatattat
    25021 ggacaatttg ttcaggacgt tattttctac atttacccat cttcgtacct catccactat
    25081 cttgctcgta ggtgagcagc actggcgcaa cgcgctttag tcctttcttc tgcctaagct
    25141 tgccctatgc gcagggcttg accactttac tttggtttcg ctgaattaag cgaaaattaa
    25201 aatagttctc ttgcaggaga aggacaatgt atatttattg gattttatta ggtctggcta
    25261 ttgctacaga aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt gagggaaatg
    25321 gcggctttat tttaatgctg gtgatgattt ctctgtcgta tatatttctc tctttcgccg
    25381 ttaaaaaaat cgccttaggc gtagcttatg cgctgtggga aggtatcggt attttattta
    25441 ttaccttgtt tagcgttttg ttattcgacg aaagtttatc gctgatgaaa attgccgggt
    25501 taaccaccct ggtcgccggg atagtgttga tcaaatcagg tacccgtaaa gcgcgtaaac
    25561 ctgaactgga ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg gctggcattg
    25621 gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg ctttcgtcgc
    25681 aaaatatttg gcttgctctc cctggcggcg gtgctggcgg cctttagtgc gctttctcag
    25741 gccgttaaag ggatcgactt gtctgtcgtt tatgcattgt ggggcgggtt tggtattgcc
    25801 gccacgttag ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa aggctggatt
    25861 ggcctggtct tgctgttggc tggaatgatc atggtgaaac ttgcctgatg aagacgctgc
    25921 ccgcgctgtc gggcagcgtt tgaacattat ttttgcgaca gttgatccag cttgtcgcga
    25981 aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc gatctttcgc ggcaggagcc
    26041 gaactttgca ccccaattaa ttgccagccg tcatcggtat gcaacatcag aggcgaaccg
    26101 ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc ccagccagtc
    26161 acttcacagt tttgatgact gtataacgta tcgagatgat cttcagggta tcccgcctga
    26221 gtcactttac gaccagccga ttttaatgcg gcagtaagcg cggctttatc tccctcaaat
    26281 aacggcaacg gtgtaatgcc agaaggggga ttacgtagca caatcaatcc gaagtcccac
    26341 ggtgcagctg cgggaggaac aatccaacca tccccatctg cttttaaccg ctttcccagt
    26401 gtcggatcaa cgcggccttc tatgtcgtgg atctcatagc gccaaagacc tttatttgac
    26461 acaaaacgca gcgctaccgc cttatccgct ttacccttag gcggcgtcaa taaacagtgt
    26521 cctgccgtta atgccagatt gggtgcaatc agcgtcgccg tacataaatt gccgctggcc
    26581 gtttccagtt gcccaaccgc atcccacggt gattgggtcg tgtcattcac tggcacacga
    26641 tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc aacgtctggt
    26701 ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac ccaacactac agcaatggtt
    26761 gtacgcatat cacactctgg tgggtaatta tgattattaa aagcaaaccc tcataaatac
    26821 tatagacggg acgggatgaa agtgggagta aaatcagata actacagtca ggaaagataa
    26881 aaacgggcgg caatcagtgc gcataaaata aggatgataa gaatgaactc aaagcgataa
    26941 cggcccacca tactaccctc cgctaaaggc ggtgctaaac gttacccgct tagcaccgta
    27001 attttcaggc gcgagggggc gcgccagcat tactgttgaa aacttacgct gcgggttgtg
    27061 cagcaggttt tgccggttgc tgatggccgt gtttcccggc gtgtttcttc gctgcctgcg
    27121 ctttttgttc aggggctttc tgttcagctt tcgtattttt atgatgcttt ttagccgcct
    27181 gcgctttctg ggcaggtgct gctttatgct gttttttatg atgtgtagtt ttcgctggcg
    27241 ctgctttggt ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca gcaaaggcgg
    27301 cagaagacag acccatagca gcggcaacaa ccagagctaa tacttttttc attgtcatac
    27361 cctcaatttg ttttttcatt taaccccact gcggggccgt tgaaataact atatccctgg
    27421 gaatggcaga cttccgtgag tggttggttt cagcgtgtaa cgatatgtac aaacgctgaa
    27481 taaattacag cgttgataat aacgtattgt gacttaagga aaatttagct accaataata
    27541 gtagtcttga tcgggttaac tattatacta cttacaaagc ggtagaataa ctgcgcatca
    27601 gtaataaatg acacacagca aaatgaatcc gtttatttgg gtacttataa tcctgatgac
    27661 gctagatgcg ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc tattaacgtt
    27721 ggtttgagca ggcaataagt ccggacgggt atttaccgca gtccggactt atttttcagg
    27781 cgtgcagacg acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc atcagagcaa
    27841 taaatgcgcc gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg
    27901 caatactcga ccccagatag taactgaaca gatacagcga ggaggcctgg cctttagcgc
    27961 gttttgcgcg ggggccgatc cagctgctgg ctactgagtg ggctgcgaag aatccggctg
    28021 agaagagtaa cattccggca aagatcagcc acagcgagct aaataaggtc atcagtaaac
    28081 caaacggcat aactcccgtc gaaaacaaca tcaccggacc gcgcccatag cgggtggtca
    28141 tggttccggc tttgggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa
    28201 ccacggcctg actgacatgc tagggggaaa gcatcaaccg atagccgatg taattaaaca
    28261 gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt
    28321 cacgccagtg cagacgaaag ttgataaata acgtcttagg gcgcagcgaa gtcgggcaaa
    28381 aatggcgtga ttcagggagg attttccaga acatcaacgc cgaggccagc gcgaaacaac
    28441 cgattgccgc cagagcaatt cgccagttga aaaagtccgt gaagacacca ctaattaagc
    28501 gtccgctcat gccgccaatt gagttgccgc tgatatacaa ccccatcgaa aaggccacga
    28561 aactggggtg gatttcctcg ctaagataag tcatgccaac agctgccacg ccacttaacg
    28621 aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa
    28681 gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg
    28741 catcggatag cgggccagta aatagcaaac cgatagccaa catcgccgtg gaaatggaca
    28801 gtgaaatact actgttcgcg ggcgttaagc caaactcctg cgaaagcacc ggaaggatag
    28861 gctgcacaca atagagaagt gcaaaggttg ccagtccggc agagaacagc gccagggtga
    28921 cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa atgctttgct
    28981 tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt
    29041 gctaaatatg cctgtggatc aggcttatac atagggtagg aaaatcgaat tgttctgtct
    29101 aatatattaa taatctcaaa taagatgttt taaatatgaa tattgaactt cgtcatctgc
    29161 gttactttgt tgctgttgcg gaagagctgc attttgggcg cgcggctgcc cgcctgaata
    29221 tttcgcaacc gccgctaagt cagcagattc aggcactgga gcaacaaatc ggtgcccgac
    29281 tgctggcacg aactaatcgc agtgtattgc tgacggcagc aggaaaacag tttcttgcag
    29341 atagtcggca aatcctgtct atggtggatg acgctgccgc tcgcgctgaa agactgcatc
    29401 agggtgaagc gggggagttg cgtattggtt ttacttcgtc cgctcctttt attcgggcgg
    29461 tgtccgatac gttatcgctg tttcgccgtg attatcctga tgtccattta caaacccgcg
    29521 aaatgaacac tcgcgagcaa atcgctccgc tcattgaagg aacgctggat atgggattgc
    29581 tgcgtaacac agcgttaccg gagacgcttg aacacgcagt catcgtccat gaaccgctta
    29641 tggcgatgat cccgtacgat catcccctgg caaataatcc gagtgtaaca ctggctgaac
    29701 tggctgaact ggctgaactg gcgaaagaac cctttgtctt ttttgatccg cacgtcggga
    29761 ctgggctgta tgacgatatt ctcggactga tgcgacgtta ccatttgacg cccgtcatca
    29821 ctcaggaggt gggcgaggca atgaccatca tcggtctggt ttccgccggt ctgggtgttt
    29881 caattttgcc tgcgtcattt aaacgtgttc agctcaacga aatgcgctgg gtgccgattg
    29941 ctgaagagga tgcggtttct gaaatgtggt tggtctggcc gaaacatcat gaacaaagtc
    30001 cagctgcgcg taactttcgt attcatctgc tgaatgctct caggtgaggg aaatttcagc
    30061 gaaaaagccc gaaaaatgtg ctgttaatca catgcctaag taaaaatttg acgacacgta
    30121 ttgaagtgct tcaccatagc ctacagatta tttcggagcg cgaaaatata gggagtatgc
    30181 ggtggttgct gaaaaccagc ctgggcacat tgatcaaata aagcagacca acgcgggcgc
    30241 ggtttatcgc ctgattgatc agcttggtcc agtctcgcgt atcgatcttt cccgtctggc
    30301 gcagctggct cctgccagta tcactaaaat tgtccgtgag atgctcgaag cacacctggt
    30361 gcgagagctg gaaatcaaag aagcggggaa ccgtggccgt ccggcggtgg ggctggtggt
    30421 tgaaactgaa gcctggcact atctttctct gcgcattagt cgcggggaga ttttccttgc
    30481 tctgcgcgat ctgagcagca aactggtggt ggaagagtcg caggaactgg cgttaaaaga
    30541 tgacttgcca ttgctggatc gtatcatttc ccatatcgat cagtttttta tccgccacca
    30601 gaaaaaactt gagcgtctaa cttcgattgc cataaccctg ccgggaatta ttgatacgga
    30661 aaatggtatt gtacatcgca tgccgttcta cgaggatgta aaagagatgc cgctcggcga
    30721 ggcgctggag cagcataccg gcgtaccggt ttatattcag catgatatca gcgcatggac
    30781 gatggcagag gccttgtttg gtgcctcacg cggggcgcgc gatgtgattc aggtggttat
    30841 cgatcacaac gtgggggtgg gcgtcattac cgatggtcat ctgctacacg caggcagcag
    30901 tagtctcgtg gaaataggcc acacacaggt cgacccatat ggtaaacgct gttattgcgg
    30961 gaaccacggc tgcctcgaaa ccatcgccag tgtggacagt attcttgagc tggcacagct
    31021 gcgtctcaat caatccatga gctcgatgtt acatgggcaa ccgttaaccg tggactcatt
    31081 gtgtcaggcg gcattgcgcg gcgatctact ggcaaaagac atcattatcg gggtgggcgc
    31141 gcatgtaggg cgcattcttg ccatcatggt gaatttattt aacccacaaa aaatactgat
    31201 tggctcaccg ttaagtaaag cggcagatat cctcttcccg gtcatctcag acagcatccg
    31261 tcagcaggcc cttcctgcgt atagtcagca cattagcgtt gagagtactc agttttctaa
    31321 ccagggcacg atggcaggcg ctgcactggt aaaagacgcg atgtataacg gttctttgtt
    31381 gattcgtctg ttgcagggtt aacatttttt aactgttcta ccaaaatttg cgctatctca
    31441 atttgggcca ggaaagcata acttagactt tcaaggttaa ttattttcct agtttatatt
    31501 tgtgaagcat aacggtggag ttagtgatgc tgaagcgttt ctttattacc ggtacagaca
    31561 cttctgtagg gaaaacggtg gtttcccgcg cattgctaca agcgttagcc tcccagggaa
    31621 aaagggttgc gggatataaa cccgtagcga aggggagcaa agagacaccc gaagggctgc
    31681 gtaataaaga tgccttggtg ttgcagagtg tttcaaccat cgaactgcct tatgaagcag
    31741 ttaatcctat cgcgttaagc gaagaagaaa gtagcgtggc gcacagttgc ccaatcaatt
    31801 acaccctcat ttcaaacggc ctggcaaacc tgaccgataa agtcgatcat gtcgtggtag
    31861 aagggactgg cggctggcgc agtctgatga atgatttgcg tccactctct gaatgggtag
    31921 tgcaggaaca actgccggtg ttgatggttg tcggtattca ggaaggttgc attaaccatg
    31981 cactgctaac agctcaggcg atcgccaacg acgggctgcc gctcattggc tgggtggcta
    32041 accgaatcaa cccaggactg gcgcattatg cggaaatcat tgatgtgctg ggtaaaaaac
    32101 ttccggcacc gctcattggt gaactgcctt atctgccgcg cgccgaacag cgtgaactgg
    32161 ggcaatacat ccgcttagct atgctgcgca gtgtgctggc ggtagataga gtcacggtgt
    32221 aacgtccgcg aaattaccga cgcaattacg caggcaatca ataaaccggg gagtagctga
    32281 tactccccgg tcatttcaca tatcatcaac gtcgacataa tcggcgcgtg cgtggttgcc
    32341 gccaacagtg tcgccatccc ggtcaatccg agtaaaagcg taatttcttc gccatcaggg
    32401 aaccataatc ccaggctacg accatacaac atgccaatgg caagaccgat aaatagcgtc
    32461 ggtgtaaaga caccaccggg tgcgccggaa ccgctactcg ccagcacggc acacagttta
    32521 caaaggaaga tcccggcaat gatcattaac agtggtgggg cggttaaaaa ggattgtacg
    32581 gtgctatagc cgttgcccca cactgcaggt gtgaacaggg aaagcagacc cacgatcaac
    32641 ccgcctagtg ccagttgcca gggcggcgca agtttgagac tcacaaatcc acgatgacag
    32701 gcgttcatta acgttaacaa cagtggtccg cacagacctg ccagcacacc tgtactgata
    32761 atcagcgcat agtcacgagc ctgaaccgtc actgagagtt gtacgttgta gagtaacgcg
    32821 tcgctatgat taatcagatt gctaaccagc catgccacga tggcggaaat aatcaccggg
    32881 ccgagagagg ccaacatcat agtgccaaac agcacttcgg ctataaataa actgccagca
    32941 agcggggcac gataggccgc agccattccc gccgcggccc cacaggcgat ccataatttc
    33001 cattcctggc gtggcgtaaa acgttgggca aaacaggagg cggcaagggc agctaaaaga
    33061 atcatcgcac cttcgcgacc aattgcactg ccgctggtta ctaccagcag cgaggcaagc
    33121 gatttaacca ggcttgctgc gtaatcgaac tgtccatcgg tttgcaacgc ttccatgtaa
    33181 tcggtcggcg catgagggcg ttgttgggta aatttctgcc agcccatcag caacaaaccc
    33241 gccgccagtc cgccgagcgc cggagttagc agccgtcgcc aggaggaaag gtttgtcgct
    33301 gcattgacca gactgccgga gtcattattg aggaacaacc actccagtag cagcatcgca
    33361 tgacgaaacc cggcaacggc aaagacagcg agaataccga cgactgttgc gataagcaga
    33421 cggcggaaca tagtgcgcag atccggatag atgtgtagat gatgcatgag cagaaatggg
    33481 caatgaaaca cgggcgacta ttttgcggca aaagaggcca accgcaaaag cagtgcgtgc
    33541 tttaagagat aaaaaaacgt gacattgtaa cgtattattg ccgaatcgcg cgtaatcgtc
    33601 accatccggc aatattacgg tgatcctatc gaaagagtgg tttaaccgcg acaggaatca
    33661 acagttgtga ttgccattgc gccagcgtta agcgagccag ttcacccagt gcacggtaga
    33721 aagggtgttc tgctttttcg ataaaaacat caagaaaacg tgttgaccac ggaaaaaggt
    33781 gccatgccag caattcttcg cattctgtct ggcgaccatt ctccgccaac cacgcagcca
    33841 tcaacagcag tgaaccaaaa tgatcttccg gttcgttttg cttcatttca aactgaatgc
    33901 ctttctcgcg catccactga cgaagtgcca atgttgaatc gccaaacagc acagattcgc
    33961 gatccagcca gaccgacccc cacggcggag acggcagtgc ccacgggccg acaaacaaac
    34021 gctgccaggc ctgggcgtga gtctcttcac actgtgtctg aaaagccgtc accagcggcg
    34081 ctaatgacgc ttctggtaga ggccactgag tttcccagcc gtcactggtg agtaccgcaa
    34141 ccagaggtgc ggcttccgca ctctctggag cgtaataaaa cagcgcgccc aacacgcgcg
    34201 ccgcgacaga aaaattatct tgctgtgaaa aatgggtcat tcctaacatc ctgaaatgtg
    34261 cgggttgccc cgcaccaatt tgttaacctg caatagccat tcctactgtc atgtgcaggc
    34321 cataaaagag cacacgcccg atcatctcac caacaagtat gaggattagc cccagaacaa
    34381 gtccggcaac gtgaggttca cggcgacgaa tgagcggaca aagccagcat ccaagacctg
    34441 cgcataacaa taccacacgc cagacctgta aggaggcgta atctggtacc agtgcgctgg
    34501 cttgttgcac ggatgagtga atagacgcca gagacaaacc ttgcagcaca atcacctcgg
    34561 cacaagcgat cagcgccaga acgctaatga tggcaaatgg tgtggtatta aatgtaacgc
    34621 gggctgcccg cagaattgcg gcagcaagta ttggaccgct caacaatacc gtcaggaaga
    34681 aagccagcgt agtgtaaccg ttatgccagg ttggcacggt gtcgatttga tacacgcagg
    34741 tcatcatcca gacgaaaatg acgccgagcg ccatgctgac aagtaaccag agtttcccca
    34801 acgcttgcgg cattttaccg atgaccgcca ccagccacca tagtccgcca acggcaaaaa
    34861 atatggaacc ggcagcaatc tcgttactca gaccagaagc tccgattcga ttcagcgagt
    34921 taaacgctcg cagtggcgat cccaggtgca tgacagaggc aataaacccg atgcccatca
    34981 acagccagag gaaaaacatg ccacgaacaa tgtgctgacg gtctgcgtca tttttggcgg
    35041 cgaaccagcc aattccgcta acaatcagcg cgcctactac gcattgaccc aatacggtaa
    35101 agataaccag tggccattca tgccatccat ttcccatttt acacttcctc cggattagcc
    35161 agataaccag tggtatcgcc ggtcgggcgg ctgttggcgt taggtttgat aacgatattg
    35221 ggttttgtga aatgcgcgcg cggcagtggc gcgacggcag ccagagtgcc gtgcttctgg
    35281 cgcagctctt caattggacc gaactcaagt gcacgcagcg ggcaggattc gacgcatatg
    35341 ggttgtttcc cctcggcgac gcgcgaataa caaccatcgc acttcgtcat gtgccctttt
    35401 tcagcattgt actgtggtgc gccgtacgga caagccatgt ggcagtagcg gcagccgata
    35461 caaacatctt catcaaccac cacaaagcca tcttcacgct tatgcattgc gccgctgggg
    35521 cagacttttg tacatgcggg gtcatcgcaa tggttacagg agatggagag ataataagcg
    35581 aacacgttct ggtgccagat gccattatct tcctgccagt cgccgccagc atattcataa
    35641 atacggcgga aactgacttc cgggccaaga tctttaaaat ctttgcacgc cagttcgcag
    35701 gttttacagc cagtacagcg gctggaatcg ataaaaaatc catattgtgt ggtcatgggc
    35761 tactccttaa accttttcga tctggacaag attgctgtgc gacgggtttc cctttgccag
    35821 cggtgaaggg cggtgagagg tcagaatatt gatactgccg ccgtgatcga cccggtcacc
    35881 aaacatatcc gctttaagcc acgcaccttg cccgatggcg gtaacgccag gcagaatacg
    35941 cggagtcact tttgcggcaa tcagcatttc tccattattg ttaaataccc gcacggtatc
    36001 gccatgacgg ataccgcgtg cctgagcatc aatggggttg atccacacct cttgtgggca
    36061 ggcctgctgt aacacatcaa tattgccgta gctggagtgg gtacgcgctt tggtttggcg
    36121 acttcactca gcatccagta gatgggtttg cgttcaaatt ttgctgaggt tgcgggttgg
    36181 gcgaggatca catagcccat attccctgca gattcatgag agataaggtc ttcttgctct
    36241 gttggcatca ggtcaggcaa caggatatcg caatacttag ccgaggccgt catgaagtgg
    36301 tcaatgccaa caatcatctc gcacttgctg tcatcctgaa gcacctcatg ggtgtgattg
    36361 atgtcgccat gttgattgat caatgtgtta ctggcgtagc accataaaaa cttgatgggg
    36421 acatccagtt tttcttttcc acgaacacca tcacgggtcg cggtcatttt cgtaccatgg
    36481 tcgatggcat ctgtccatgt aaaaacggaa atctgcgttt taacaggatt ctcgagcatc
    36541 gggaaccatt ctacccccag atcccagcta ccttcgcgta cacctgagtt gccgccgttt
    36601 atgccgacgt taccggtaag aacggaaagc atggcaatag cgcgggacgt ttgttcgccg
    36661 ttggagtgtc gttgtggccc ccaaccctga caaatataag caggttttgc tgaaccgatc
    36721 tctcgtgcca actggataat tttttctgcc gggatgctgg tgatttttgc tgcccattcc
    36781 ggcgttttag ctatgccgtc aggcccttcg cccagaatat aggctttata atgcgcgtta
    36841 cgtggtgcgt tggcgggcag cgttttttca tcgtaaccaa cacaatattt gtcgagaaat
    36901 ggctgatcga ccatgttttc agtaatcagt acccaggcaa tcgcacaggc cagtgcgcca
    36961 tcggtgccag ggcgaatggg cagccattca tcttcacgcc cggcagctgt gtcgttatag
    37021 cgtggatcga tgacgatcat gcgtgcgttt gaacgttcgc gggcttgctc gacgtagtaa
    37081 gtgacaccac cgccgctcat ccgcgtttct gccgggttat ttccgaacat aacgaccagt
    37141 ttcgtattgg cgatatcatc cgggctgttg ccatcattgg caccgaacat ataactcatt
    37201 gcggcactga tctgtgcggt actgtagctg ccatagcgac tgagaaaacc cccgcaagag
    37261 ttcatcagac ggtacgggac gttcgagttg gtgatatttc cgccatctac gcctgttccg
    37321 tacaggacat gtacagcctc attgccgtaa tctttcagga tccgccgaag attatcactg
    37381 atggtatcca gggcttcgtc ccaacttatc cgttcaaatt taccttcacc gcgctcgccg
    37441 acgcgcttca tgggatattt caacctgtca ggatgattca tccgtcggcg gatagagcgc
    37501 ccgcgtaaac atgcccgaac ctgatgatta ccgtaaacgt cgtcacctgt cgtatcagac
    37561 tccacccagt acacggtgtc atctttcaca tgcaaacgta gcagacagcg gctcccgcag
    37621 ttaacggtgc aggaactcca gaccgttttc tcttctaccg gagcctctgc cgcccggacc
    37681 atttgggaaa atggcagagt gaaagcactg cttgccagcg cgagactgcc aagtgcggag
    37741 gttttcatca gacttctatg gctgatttca gccttcatga gcgcctctgt ggtatggatt
    37801 ttcatcatta ctcacttatt gcttttcaaa caaaatgtca tgccagaatt tatggttgta
    37861 gtgggttata ttttttcgat ctcgaccaga ttagtgtgct gcgggtttcc cttcgccagt
    37921 ggtgaagggc gcagagtggt tagcgtattc acacatccgc catggtcgat tttatcgcca
    37981 gacatattgg gctcgtgcca ggctccctgg cccatagcgc taactccagg gagaatgcgt
    38041 ggtgttactt tggctggtag ccgaacttcg ccacgatggt taaacacccg caccatatcg
    38101 ccgttggcaa tcccacgttt ctgtgcatct atagggttga tccacacctc ctgacggcag
    38161 gctgccttca ggacatcaat gttgccgtag gtcgagtgag tacgggattt gtaatggaaa
    38221 ccaaacagtt gcagtgggaa ggttctacgt tcaggggagt cccagccttc aaaggttgag
    38281 gcataaactg gcagtgggct tatcacttca tctttttcca gttcccaggt acgggcaatt
    38341 tccgccagct tgctggaata aatttcaatc ttaccggaag gcgttttaag tggatttgcc
    38401 tcggggtcgt cacgaaatgc tttgtaggcg acaaaatggc cattgggatc tttacgctta
    38461 tagataccca tttttttcag ttcgtcgtaa gacggtaacg ccggatcttt ggcaagcatt
    38521 ttggcgtaca gatgttgtaa ccattgttcc tgcgtgcgac cttctgtgaa cttttgatag
    38581 acgtcaggtc caagacgttt cgcgacttca ctcaggatcc agtaaatcgg tttacgttcg
    38641 aatttttcgc tggtgactgg ctggaggaaa atgagatatc ccatgttacc ggcgtagtcg
    38701 ttaggaataa tatcttcctg ctcaacggtc atcaggtctt ccagcagaat gtcggcatat
    38761 tttgccgatg aggtcataaa gttttcgatg accacaatca tttcgcattt cgattcgtcc
    38821 tgcagaattt catgcgtttt gttgatgtca gaatgctgat taacgagggt atttcccgcg
    38881 tagttccaga tggactccat tgccagacgg atggatggac ttgaagtagt tgactcaatg
    38941 ctacttaagg tcctgatatg actgacatta atcatgataa cactgtatat ccatccagtt
    39001 ctttctctgg gtctccctat cggtatcagc aagtctacac ctcaagccct gacacaaggc
    39061 tgacacaaat caatctactc agcgtagcta agcctcagtt agtccgcaga gccaatggtc
    39121 gatatactat ccgcttcaga ctcaagggac agaccacacc gtttctttca gtatcgaccc
    39181 gcagcacaga caggagagta gccacaatgc gtcagagaga gcttgcagcc accgctaagg
    39241 ccttcatgtt ggataggcct gaagtctcac tacaggagct tacagagcat ctccggtcta
    39301 tggctgagca gttcctgaca gacgctagtg acgattactg gaatggactt gaggtcgcta
    39361 ctctggtcga cgagaagtcc aacctgaagg agctagccgc tacgcaagcc ttgagtctgg
    39421 accagcagaa gggcataagg ttagcgctgg aagtcctgac cgcagcacag cagagagtcg
    39481 acactggtga tacctctggt ctgattaagc tgattgatga taataacctt actgatgact
    39541 caactatagg cgactctacg tcaattctga ataatgagca gggggataga ccggcagttt
    39601 tcacacagga gcgccagtca tctgtagttt tttcaagcct agtgtcctcg ctgctggctg
    39661 agaaggtcca gaccctgaag acatccagct acaaggacct ctcgtcctca ctcaatactg
    39721 tctcccggtt cctgcctgaa gacatggacc tgatgtcccg ctctgggtgg ctggctgtca
    39781 gggactctat gctggcttct gaggtgagac catcgacaat caacaagctg ctgaccaaag
    39841 cgaagatgtg tctcgattat ggcctgatga atgggcaact tgagggccgc aaccctattg
    39901 aacggatgaa gctgaccaag gatatcgact ccaagcgcag agcgttcaca gacgaggagc
    39961 tggagagact tctggtccgt gttgagt
//
LOCUS       DAJTSA010000029        37627 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000029 DAJTSA010000000
VERSION     DAJTSA010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37627
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.029"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..175)
                     /locus_tag="OO369_001623"
     CDS             complement(<1..175)
                     /locus_tag="OO369_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR5981938.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="OO369_001624"
     CDS             complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="OO369_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5981939.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1229..1525)
                     /gene="fis"
                     /locus_tag="OO369_001625"
     CDS             complement(1229..1525)
                     /gene="fis"
                     /locus_tag="OO369_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR5981940.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="OO369_001626"
     CDS             complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="OO369_001626"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR5981941.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="OO369_001627"
     CDS             complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="OO369_001627"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR5981942.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3738..5189)
                     /gene="panF"
                     /locus_tag="OO369_001628"
     CDS             complement(3738..5189)
                     /gene="panF"
                     /locus_tag="OO369_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR5981943.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5179..5421)
                     /locus_tag="OO369_001629"
     CDS             complement(5179..5421)
                     /locus_tag="OO369_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR5981944.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5530..6879)
                     /gene="accC"
                     /locus_tag="OO369_001630"
     CDS             complement(5530..6879)
                     /gene="accC"
                     /locus_tag="OO369_001630"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR5981945.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6890..7360)
                     /gene="accB"
                     /locus_tag="OO369_001631"
                     /gene_synonym="fabE"
     CDS             complement(6890..7360)
                     /gene="accB"
                     /locus_tag="OO369_001631"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR5981946.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8338..9312)
                     /locus_tag="OO369_001632"
     CDS             complement(8338..9312)
                     /locus_tag="OO369_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5981947.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9464..11404
                     /gene="csrD"
                     /locus_tag="OO369_001633"
     CDS             9464..11404
                     /gene="csrD"
                     /locus_tag="OO369_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR5981948.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11709..12752
                     /gene="mreB"
                     /locus_tag="OO369_001634"
     CDS             11709..12752
                     /gene="mreB"
                     /locus_tag="OO369_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR5981949.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12818..13921
                     /gene="mreC"
                     /locus_tag="OO369_001635"
     CDS             12818..13921
                     /gene="mreC"
                     /locus_tag="OO369_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR5981950.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13921..14409
                     /gene="mreD"
                     /locus_tag="OO369_001636"
     CDS             13921..14409
                     /gene="mreD"
                     /locus_tag="OO369_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR5981951.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14418..15011
                     /gene="yhdE"
                     /locus_tag="OO369_001637"
     CDS             14418..15011
                     /gene="yhdE"
                     /locus_tag="OO369_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR5981952.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15001..16470
                     /gene="rng"
                     /locus_tag="OO369_001638"
     CDS             15001..16470
                     /gene="rng"
                     /locus_tag="OO369_001638"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR5981953.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16538..20338
                     /gene="yhdP"
                     /locus_tag="OO369_001639"
     CDS             16538..20338
                     /gene="yhdP"
                     /locus_tag="OO369_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR5981954.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20494..21939
                     /gene="tldD"
                     /locus_tag="OO369_001640"
     CDS             20494..21939
                     /gene="tldD"
                     /locus_tag="OO369_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR5981955.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="OO369_001641"
     CDS             complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="OO369_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR5981956.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23179..23382
                     /locus_tag="OO369_001642"
     CDS             23179..23382
                     /locus_tag="OO369_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR5981957.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23390..24322
                     /gene="aaeA"
                     /locus_tag="OO369_001643"
     CDS             23390..24322
                     /gene="aaeA"
                     /locus_tag="OO369_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR5981958.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24328..26295
                     /gene="aaeB"
                     /locus_tag="OO369_001644"
     CDS             24328..26295
                     /gene="aaeB"
                     /locus_tag="OO369_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR5981959.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26387..26659
                     /locus_tag="OO369_001645"
     CDS             26387..26659
                     /locus_tag="OO369_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR5981960.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="OO369_001646"
     CDS             complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="OO369_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR5981961.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27343..27813)
                     /gene="argR"
                     /locus_tag="OO369_001647"
     CDS             complement(27343..27813)
                     /gene="argR"
                     /locus_tag="OO369_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR5981962.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28248..29186
                     /gene="mdh"
                     /locus_tag="OO369_001648"
     CDS             28248..29186
                     /gene="mdh"
                     /locus_tag="OO369_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5981963.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29248..30315)
                     /gene="degS"
                     /locus_tag="OO369_001649"
     CDS             complement(29248..30315)
                     /gene="degS"
                     /locus_tag="OO369_001649"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR5981964.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30405..31752)
                     /gene="degQ"
                     /locus_tag="OO369_001650"
                     /pseudo
     CDS             complement(30405..31752)
                     /gene="degQ"
                     /locus_tag="OO369_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31906..32304)
                     /locus_tag="OO369_001651"
     CDS             complement(31906..32304)
                     /locus_tag="OO369_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR5981965.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32498..33625
                     /gene="zapE"
                     /locus_tag="OO369_001652"
     CDS             32498..33625
                     /gene="zapE"
                     /locus_tag="OO369_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR5981966.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33844..34272
                     /gene="rplM"
                     /locus_tag="OO369_001653"
     CDS             33844..34272
                     /gene="rplM"
                     /locus_tag="OO369_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR5981967.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34288..34680
                     /gene="rpsI"
                     /locus_tag="OO369_001654"
     CDS             34288..34680
                     /gene="rpsI"
                     /locus_tag="OO369_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR5981968.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35075..35713
                     /gene="sspA"
                     /locus_tag="OO369_001655"
     CDS             35075..35713
                     /gene="sspA"
                     /locus_tag="OO369_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR5981969.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35719..36216
                     /gene="sspB"
                     /locus_tag="OO369_001656"
     CDS             35719..36216
                     /gene="sspB"
                     /locus_tag="OO369_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR5981970.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36259..37626)
                     /gene="dcuC"
                     /locus_tag="OO369_001657"
     CDS             complement(36259..37626)
                     /gene="dcuC"
                     /locus_tag="OO369_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5981971.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVDGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMPFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         9114 a         9412 c         9901 g         9200 t
ORIGIN      
        1 ttctgtcctg aaaattcagt tcgtcacagt taaccagttt tagtggatta actgagttca
       61 tattacggca tttgtctgtt tctgttgtca gcagttcaat ccactgcatc agtaaagtat
      121 caaacagaaa aacgaagaca gcgaaaacga cgagccacca atacttgcga atcattacct
      181 gagcctgtgc atgtttaacc aagtctggca ggaaatatac acgaaatgaa taatctgaaa
      241 actaggctta cacttccttt actttgcaat gagatcgggg tcaacttcgc ttaaccggct
      301 tcgttttgac aggttgccgg tctttctttt tttcactttc gccagttctt ccgcggagta
      361 atgcgacgcg acatccagcc gtcgaagccc cattttgatg tactcgctgt tgatctcaat
      421 accaatgaat tttcgtccgc tggcgatggc tacggcaccg gtagtaaagc taccggcaaa
      481 cgggtcgaga acgatatcgc ctgggttgga agaggcgaga ataatgcgtt tcagtaaggc
      541 tttcggtttt tgcgtcgggt ggttctcata ttcatccatc aaataacgca cgcgcggaaa
      601 atcccagacg ttacccggta ctttttgatg attgtatggc tgtggaggat ttttgcgata
      661 atcgatcaac gcgcgctgcg atccggtttt ggcttctacc agaatagcat caccgttgaa
      721 tgtgtagttc tttgcgtctt tcaccatcat caggatgggt tcgtacatgg agccgtagtg
      781 ttttttcgcc tgtactccag aactgtcata tgaccagacg atgcgacttt tgatggtaaa
      841 aagcttgcgg cactggagat cgataaaggg catgttttcc gtactgttca taatgtacat
      901 gctgccctgc tttttcagaa cgcggtggca ctctgcaatc acttcaaaca gccagtcgat
      961 aaacagatct tctttccagg cttcgatcag accatcaaaa tttttaccga tgttatacgg
     1021 tgggtcggca aagagcagat cgacgctttc agtaggtagc tttttaagtt cggcaaaggc
     1081 atcaccgtga ataatggtct tagcttcatt accaaaccgg gtcggttcac atcctgttct
     1141 catggtcact ccctttgtga cacctataaa aaaggcgctt ccccatgccg agtagcgcct
     1201 ttttaatcaa gcatttagct aacctgaatt agttcatgcc gtattttttc aattttttac
     1261 gcagcgtacc acggttgatg cccatcatca gcgcagcacg ggtctggtta ccacgggtgt
     1321 attgcatcac catgtccaac aggggctgtt ctacttcagc cagtaccagc tcatagaggt
     1381 cattcacatc ctgaccattc agttgagcaa aatagttctt cagtgcctgt ttaaccgagt
     1441 cacgcagggg tttttgggtt acctgatcct gagagttaac ggtagaaacg gtcagtacgt
     1501 cagaatttac gcgttgttcg aacatagttc tgtcagctct ttatttctgt ttacgcaaaa
     1561 ttttcgaagt atgcctccaa cgcctccagc tgttcgctgg catcctcaat ggcgttgaat
     1621 gtgcgccgaa actggtcatt tggagcgtgt tcctggagat accaggaaac gtgtttacgt
     1681 gcaattcggt acccttttgc cggaccataa aagtcatgca gttcccgaac gtgcgcgcaa
     1741 agcaagcgct taacctctgc caaaggcagt gggggcagca actccccagt gtccagataa
     1801 tgctggattt cccgaaagat ccagggtctt ccctgagctg cgcggcctat catcagggca
     1861 tccgcccctg tatagtcgag cacagctctg gctttaagcg ggtcagtaat gtcgccattc
     1921 gcgataaccg gaatggaaac tttctgctta actgcccgaa tactgtcgta ctcagcttct
     1981 ccattgaaca aacaggcgcg tgtacggcca tgaatggtca gagcctgaat gccacagtct
     2041 tcagccagtt gggcaatctc ttcgcagtta cggtgttccg gtgcccagcc ggtgcgaatc
     2101 ttcagggtaa caggaacgtc cactgcattg acgacctcgg taaggatcga tttaacgaca
     2161 tccgggtact gcaagagggc tgaacctgcg agcttgcgat tcactttttt agccgggcaa
     2221 cccatattga tatcaataat ctgggcaccg ctttccacgt taatacgtgc tgcatctgcc
     2281 atttctttcg gagcgctacc agcaatttgc acggtgcgaa taccgggttc atcaatgtgc
     2341 accatccgta aacgagattt gtcgctttcc caaacctgtg ggttagaaga catcatctcg
     2401 gatactgtca atccggctcc catctcgtag cacaacgtcc gaaaaggtct gtctgtaatg
     2461 ccagccatgg gcgctgcgat caggcgattt ctgagctgat attgtccgat gcgcatgagt
     2521 taagaaatga ccatactgtg actgcaaggc ggcgtatatt acgcattttt tgcacgagat
     2581 gaaaggccaa actttgacca atcctctgag atggatcaaa gaattgcatt taaaatgagc
     2641 gtggtgcgat aattactcat aaaaatcatc atattagaaa atagtgacta aaatttacac
     2701 tcaaagaaat ttgagtaagt tctcaatttt tctttatgaa tgaaaatttt ggcacgcaaa
     2761 tttgcgtaaa taatcggcaa atttacgtgc cttttgtgag cttgctcgca cttcgccccg
     2821 cgtcacccta cggcgatgcg aaggtcaatt cttacgaccg gtaatacggc accactcttc
     2881 tttttccacg accgggtcca gtgcgaagct atcggcataa gcttcacaaa cgctctctgc
     2941 ctggcttgcc agaataccgg aaaggcccag caaaccgcct gaaactggca ggacgctgat
     3001 taacggtgcc agttcacgta atgggcctgc aaggatgtta gcgaccacca cgtcggcttt
     3061 catttcttct ggctgatctt tcggtaagta gagttccaga cggtcagaaa cgccattacg
     3121 ttcggcgtta tcgcggctgg cctgaatcgc ctgcggatcg atatcaatac caatggcttt
     3181 tgctgcaccc agtttcagcg ccgcgatcgc cagaatgccg gaaccacagc caaagtcgat
     3241 gactgtttta ccggttaaat cgaggctgtc gagccattgc aggcacagag aggtggttgg
     3301 atgggtaccc gtaccaaacg ccagccctgg atctaacatc acgttgacgg cgttttcgtc
     3361 cggcacatca cgccagctag ggcagatcca cagtcgttca ccaaagcgca tcgggtggaa
     3421 attatccatc cattcgcgct cccagtcttt atcttctagt tgttcgattt tatgcgcgaa
     3481 gcctgcgccg agcagcggat ggttttccag aatcgccacc acgtcgttca tatcggtttc
     3541 agcgtcgaac agaccaatca catcggtgtc gccccacagg cgcgtttcgc ccggcagcgg
     3601 ctcaaatact ggcgtatcgt gggtatcctg aaaagtgata gaaacggcac ccgcttccat
     3661 cagcgcatcg ctaagatctt ccgcgttcgc gccggtggtg ttcagtttca gttggatcca
     3721 aggcatggca aaactcttta tttatcagta gtcaaaacgg tagcttgcgg gacggatgta
     3781 ccgaaacggt ttccgaccag gaaagccagc aaacttagta gtaacgaggg cacgataggg
     3841 tggaagccca ggtactgaat attcagcgtc gcgagtacgg catacagcac gccgccaacg
     3901 atcatcgcac ttagcgcgcc tttggcgttg gcgcgttccc agtaaagacc cagcaccagc
     3961 ggccacagga aaacggcttc cagcccaccg aaggccagca aattcagcca gatgatcatt
     4021 tctggcggct tccaggcggc aagcagcagc aacgcgccga gaactaacgt aattaccgcc
     4081 gacatccgct tcagacgcgt ctcgttttgc atttgatccg gacggatatt cagatagaga
     4141 tctttaatga tcgtagcgga actttgcagc agttgggcgt taattgtcga catgatcgca
     4201 gccataggtg cagccagaaa gatcccggca gcaaaaggtg gcagcacttt taccattaac
     4261 gtcgggatca ccagatctgg tacggtgaga tcggggatca ctgcacgacc taacgctccg
     4321 gccaggtgca taccgaacat caggattgcg actataatcg taccgatgat gatcccccgg
     4381 tgtactgctt tgctgtcttt ataagagata cagcgcaccg cagtatgcgg caggccaatc
     4441 acgccaaagc acaccagtac ccagaacgac gtcataaagg caggcgacag aatatcgtca
     4501 gcgccttgtg gcgtaaccag ttgcggatcg atggtttgca aggtctgtac tgcgttgctt
     4561 aagccgccag cggcatgtac cacgccaata agcagcacaa cggtgccaat cagcatcaca
     4621 agcccttgca tggtgtcgtt cagcacgctg gcgcgaaagc caccaaaggc ggtatataac
     4681 gcaatgctga taccaaaaat cagcagcccg gtttcataag gaatacccgc cgcggtttcc
     4741 agcaggcgcg caccgccgat aaactgcacg gtcatcgcac caacgaacgc aaccagcaaa
     4801 ctcaaactcg ccagccacac cagaagacga ctctggtagc gggcaaacag catatcgttc
     4861 agcgtcactg cattgtagcg gcgcgcaaga atcgcaaact tcttgccgag aataccgagt
     4921 gaaagccaga cagcaggaag ctgaatcatc gccagcaata cccagcccag cccgtattta
     4981 taagcagctc ctggcccgcc gataaacgaa ctggcactga tataggtcgc ggtgagagtc
     5041 atcgccagca caataccgcc catagagcgg ctgccgagga aatactcatt aaggaaggtg
     5101 ccggtgctcc gtttacgcat cgcataaacc gagataccga acaccaccac cagataggcg
     5161 accagcggta gaattacttc aagctgcatc gtcatcctcc agtgggatat cgcgatagat
     5221 aaatttcacc atcgcccagc acagtccaat aaacagtagc ggcgtcagga tgcaggccat
     5281 ctcaaaccag cgcggaaagc cggtaaaacc gggggcaacg ccaggtaagt aagcgtctac
     5341 taaccaaact gccagataca aaagggtcag ccccagcgcc cagcgggcct ctttatgggc
     5401 ctgaacaaaa cgagtgtcca ttttttgtcc ctgatgggtg aagaaagcga ggattgtacc
     5461 ttatgggggt tgtcgatccc cagtaataaa aaaggccgga aaatccggcc ttttgacgct
     5521 tttgcagtct tatttttcct gaagaccgag ttttttctcc agatagtgga tgttagtgcc
     5581 accatgctgg aagttctcgt cattcatgat gcggatctgc agatcaacgt tggttttgat
     5641 accgtcgatg atcagctcct gcagcgcatt cttcatgcgg gcaatcgcca cgtcacggtt
     5701 ttcaccgtag caaatcagct taccgatcat tgagtcatag tacggcggta cggtgtagcc
     5761 cgcgtagata tgagactccc aacgtacgcc aaaaccgcca ggtgcgtgga aacgggtgat
     5821 tttgcctggg cttggcagga aggtgttcgg atcttcggcg ttgatacgac attccaccgc
     5881 atggccgcga acgtgaactt cttcttgctt gatcgacagc ggttgaccgg cagcgatacg
     5941 cagctgttct ttgatcaggt caacgccggt gatcatttct gtaaccgggt gttctacctg
     6001 aatacgggtg ttcatttcga tgaaatagaa ctcgccgttt tcgaacagga actcgaaagt
     6061 acctgcaccg cgatagccga tatcaacaca cgctttagcg caacgttcgc cgatgtagcg
     6121 acgcagttcc ggggtaatgc ccggtgctgg tgcttcttcg accactttct ggtggcggcg
     6181 ctgcatggag cagtcacgtt ccgccagata gatagcgttg ccctgaccgt cagccagtac
     6241 ctgaatctcg acgtggcgag gattctccag gtatttctcc atgtaaacca tatcgttgct
     6301 gaaagcagct ttcgcttccg cacgggtcat ggagatggat tgtgccagtt cagcgtcgcc
     6361 gcgcactacg cgcataccgc gaccgccgcc gccgccggag gctttgataa tcaccggata
     6421 accaatgcgt ttagcaatgg cgcggttttt atccatatcg tcgcccagcg ggccgtcaga
     6481 accaggtacg caaggaacgc ccgctttttt catcgccgcg attgcggata ctttgtcgcc
     6541 catcaggcga atggtttctg ctttcgggcc gatgaagata aagccggagc gttcaacctg
     6601 ctcggcaaag ttggcgttct cggagaggaa gccgtaaccc ggatggattg ctactgcgcc
     6661 ggtgatttca gcggcgctga tgattgccgg gatgttcaga taacttttta ctgacggagc
     6721 agggccaata cagaccgttt catctgccag taatacgtgt tttagatcgc gatccgtgct
     6781 ggagtgcaca gcgacagtct tgatgcccag ttctttacag gcacgaagaa tacgcaatgc
     6841 aatctcgccg cggttggcaa taacaatttt atccagcatg ttcgcctcgt tactcgatga
     6901 cgaccagcgg ctcgtcaaat tctaccggtt gtccactttc gaccagaatt gctttcacgg
     6961 taccggattt gtccgcttcg atctggttca tcattttcat ggcttcaacg atgcacaggg
     7021 tatcgcccac gttgactttc tgacccactt cgatgaacgc ttttgcgtcc gggcttgggg
     7081 tgcggtagaa agtaccaacc atcggggaac gtacgatgtg accactgatt tccgctgctg
     7141 ctggcgcttc catggaagga acggtcgccg gagcggctgc gttagattga gctggctgct
     7201 gcatcattgg tgcagcgtaa gcttgttgca tcacagggaa acttgcggca ggagctgcac
     7261 ggctaatgcg tactgactct tcgccttcag aaatttccag ttcggagatg cctgattctt
     7321 caaccagctc gatcagtttt ttaatcttac gaatatccat gagtgggttc cgtactcttt
     7381 gtttagtgtg attgtgacag gcgtttcacc gccgtctgta aagcgtatgc cccgacagct
     7441 gtatgcatag cgataaattc cagcaggccg ggacgccggt ctattttgcc ccagagtgtc
     7501 aatgttagac ttgacggaca ttgtgcagcg atctgctgct atcctccggc aaaaaacaaa
     7561 atataccttc gttgcgccaa tgtcaccttt tccgcagcgc aaaaactgcg tcagggagga
     7621 cgaggtcgca cattataacg atttcgtagc aattggcagc taaatactgg tcttatcagg
     7681 gaatataatc aacagctaac atgtaaataa ccttcaacac cgtgtaattt gcaacaagcc
     7741 gcacaattca cgaaattagc gccatcatcg acggaacttc ttataacgta aggtgaaaag
     7801 ctgaaaagcc agccctgcga agatgagggg ctgcaatgag ataatcttca cagaccacaa
     7861 ataatgtagg gatgctagga tcgcgacaag atagacgaca ttacgcagcg gttgctgggg
     7921 attacccaat tttcactgca tcgtctggat agatgtgaat gctaaagcac gcaaaatcaa
     7981 ccaactgaca attctcagca ttaaataggt tcgagtggtt aatttgtagt caaataaaat
     8041 cagattattc accccatggt accagaaatc agtaacaggt taagcgtagt gtcgcccagg
     8101 caaagcacca taaacctaac aggcgacgga ggcgtatcaa taatggctgt ttagaggagt
     8161 atcaatgcgc atcaatttgt ggtcagtcga aatcatgact ttccagtttg gatgatgttg
     8221 tacaacttcc tgaccaaggt gttcgacgaa agctgtccgg ctaaccgctt ttcccgttaa
     8281 ccgattgaat aatgatgtca aaatgagttc cctctcttat tattccttgc taaatggtta
     8341 gttaaccttc accagcgtgc gaccctggat ctggttatta atgatggcct cggcaaagtt
     8401 cggtgcctct gacagagata tctcttttgc cgcctgggta tagaatgatt ccggtaaatc
     8461 ggcgaccagt cgctgccagg cttgtgcgcg gcgttctggt ggcgtcatta ctgaatccac
     8521 cccttgcaaa cggacattac gcagaataaa tggcatgacc gtggttggca gagtaaaacc
     8581 acccgccaga ccacaggccg ccacgcagcc gccgtaattc atttgcgcca gcacttttgc
     8641 cagcactttg tcgccaacag tgtcaattgc cccagcccag acttgttttt ccagaggacg
     8701 ggattcggca aactcatcac gagggagaat accgctggca cctaaacttt tcagatattc
     8761 atgggtactt tcgcgaccgg aaacggcaac gacctgataa cccaacttat gcagcagcgc
     8821 cacggcggta ctgccgacgc caccactggc acccgtcacg acaatctcac cgtcctgcgg
     8881 gcgaacaccg gcatcttcca gcgccatcac acacagcatg gcggtaaaac cggcagtacc
     8941 gataatcatt gctttacgcg cgtccagccc ttgcggcatg gcaaccagcc agtcaccttt
     9001 cactcgcgcc tgctccgcca gcccacccca gtggttttca ccaacgcccc agccagtgag
     9061 taacacctcc tgaccggcat gaaaacgcgg atcttcgctg gtgcgtacag ttccggcaaa
     9121 atcgatccca ggaatcatcg gaaaattacg gatgattttt cccttaccgg taatcgccag
     9181 cgcatcttta tagttcaggc tcgaccagtg aacatcgacc gtgacatcgc cctccggcag
     9241 gcgacgttcg tccagagtct gtactgatgc gagagttttg ccgtcctgct gttctaaaag
     9301 taacgcctgc ataagtggtc ctcatgtgca tgatggattg gaaaataatt tctgaagact
     9361 atactcgcta atggaaaagc aaagccgatg aagcgcaata aattgcgaga taaatctgat
     9421 ttgctagtat gcccgcttcc tcactatcgg agttaacaca aggatgagat taacgacgaa
     9481 attttcggcc tttgttacgc tgctcaccgg gttaacaatt tttgtgactt tgctgggctg
     9541 ttcgctaagt ttctacaacg ccattcagta taagtttagt catcgcgttc aggcggtggc
     9601 gacggcgatt gatacccacc ttgtgtcgaa tgacttcagc gcattaaggc cacaaattac
     9661 cgaattaatg gtgtcagcag atatcgttcg tgtagacctg ctgcatggtg ataaacaggt
     9721 ttataccctg gccagaaatg gtagttatcg tccggttggc accagcgatc tgtttcgtga
     9781 actgagcgtt ccgttgataa agcatccggg gatgtcgttg cgtctggttt atcaggatcc
     9841 gatgggcaac tatttccatt cgttgatgac caccgcgccg ctcacggggg cgattggctt
     9901 tatcattctt atgctctttc tggcggtacg ctggttacaa cggcaacttg ccgggcaaga
     9961 attgctggaa acccgggcta ctcgtatctt aaacggtgag cgtggctcta atgtgttggg
    10021 aaccatctat gaatggccgc ccagaaccag cagtgcgctg gatacgctgc ttcgtgaaat
    10081 tcagaacgca cgcgaacaac acagccgtct tgatacgctg atccgctctt atgccgcaca
    10141 ggacgtgaaa accggcctca ataaccgact ctttttcgat aatcagttag caacgttact
    10201 ggaagatcag gagaaagtag gtacccacgg gatcgtgatg atgattcgtc tgccggattt
    10261 caatatgttg agcgatacct gggggcacag ccaggttgaa gaacagttct tcactctgac
    10321 gaatctgctg tcgacattta tgatgcgcta ccctggcgca ctgctggcgc gttaccaccg
    10381 cagtgatttt gctgcgctgt taccgcaccg gacgttaaaa gaggcagaga gcatagccag
    10441 tcagttaatc aaagccgtcg ataccttgcc gaacaataaa atgctcgatc gcgacgatat
    10501 gatccacatt ggtatctgtg cctggcgtag tggtcaggat accgagcagg taatggaaca
    10561 tgcagagtct gccacgcgta atgcgggatt gcagggtggc aatagctggg ctatttatga
    10621 tgactcgttg cctgaaaaag gacgcggtaa tgttcgctgg cgtacgctta tcgagcaaat
    10681 gctcagtcgc ggcggcccgc gcctttatca aaaaccggcg gttactcgcg aaggtcaggt
    10741 tcatcatcgc gaactcatgt gccgcatctt cgatggtaat gaagaggtta gctcggcgga
    10801 gtatatgccg atggtcttgc agtttggctt atcggaagag tatgaccgtc tgcaaatcag
    10861 ccgtcttatt ccactattgc gttactggcc agaggaaaat ctggcgattc aggttaccgt
    10921 tgagtcgctg attcgtccgc gttttcagcg ttggctgcgc gatacgttaa tgcaatgtga
    10981 aaaatcacaa cgaaaacgca taattattga acttgcagag gccgatgtag gtcaacatat
    11041 cagtcgttta caacctgtta ttcgtttagt gaatgcttta ggggtacggg tagccgtcaa
    11101 ccaggctggt ttgacgctgg taagcaccag ttggatcaaa gaacttaatg ttgagttact
    11161 caagctccat ccggggctgg tcaggaacat tgagaagcga acggagaacc agctgctggt
    11221 tcaaagcctg gtggaagcct gctccgggac cagcacccac gtttacgcca ccggcgtgcg
    11281 ttcgcgaagc gagtggcaga ccctgattca gcgcggtgtt acaggcgggc aaggggattt
    11341 tttcgcgtcc tcacagccac ttgatactaa cgtgaaaaaa tattcacaaa gatactcggt
    11401 ttaacctgcc gtttaatccg ttttcacgta gaataacgcg cgctgcgtct catgggagtg
    11461 tgcttgtctg ctcgccagat tgttgcagca catatgcaga tgaatgacct tacgcggttg
    11521 caaacaggcg aggaatgctg ctgatgcatt aagcctttct ggactcaggc agagatttgt
    11581 aacaaaggaa acgaactgca ctaattttca ccgtagcaga tgatttttgc gccttgtcgc
    11641 tgctgcgtgt ggttggtaaa gtaagcggat tttcttttcc gccccagctt tcaggattat
    11701 cccttagtat gttgaaaaaa tttcgtggca tgttttccaa tgacttgtcc attgacctgg
    11761 gtactgcgaa taccctcatt tatgtaaaag gacaaggcat cgtattgaat gagccttccg
    11821 tggtggccat tcgtcaggat cgtgccggtt caccgaaaag cgtagctgca gtaggtcatg
    11881 acgcgaagca gatgctgggc cgtacgccgg gcaatattgc tgccattcgc ccaatgaaag
    11941 acggcgttat cgccgacttc ttcgtgactg aaaaaatgct ccagcacttc atcaaacaag
    12001 tgcacagcaa cagctttatg cgtccaagcc cgcgcgttct ggtttgtgta ccggttggcg
    12061 cgacccaggt tgaacgccgc gcaattcgtg aatccgcgca gggcgctggt gcccgtgaag
    12121 tcttcctgat tgaagaaccg atggctgccg caattggtgc tggcctgccg gtttctgaag
    12181 cgaccggttc tatggtggtt gatatcggtg gtggtaccac tgaagttgct gttatctcct
    12241 tgaacggcgt ggtttactcc tcttccgtgc gcattggtgg tgatcgtttc gacgaagcta
    12301 tcatcaacta tgtgcgtcgt aattacggtt ctctgatcgg tgaagccacc gcagaacgta
    12361 tcaagcacga aatcggttcg gcttacccgg gcgatgaagt ccgtgaaatc gaagttcgtg
    12421 gccgtaacct ggcagaaggt gttccacgcg gttttaccct gaactccaac gaaatcctcg
    12481 aagcactgca ggaaccgctg accggtattg tgagcgcggt aatggttgca ctggaacagt
    12541 gcccgccgga actggcttcc gacatctccg agcgcggcat ggtgctcacc ggtggtggcg
    12601 cactgctgcg taaccttgac cgtttgttaa tggaagaaac cggcattcca gtcgttgttg
    12661 ctgaagaccc gctgacctgt gtggcgcgcg gtggcggcaa agcgctggaa atgatcgaca
    12721 tgcacggcgg cgacctgttc agcgaagagt aatcggatgc aggcagggga agcgtctgtt
    12781 taccctgcct ggtctgatac gagaatacgc ataacttatg aagccaattt ttagccgtgg
    12841 cccgtcgcta cagattcgcc ttattctggc ggtgctggtg gcgctcggca ttattattgc
    12901 cgacagccgc ctggggacgt tcagtcaaat ccgtacttat atggataccg ccgtcagtcc
    12961 tttctacttt gtttccaatg ctcctcgtga attgctggat ggcatatcgc agacgctggc
    13021 ctcgcgtgac caactagaac ttgaaaaccg ggcgttacgt caggaactgt tgctgaaaaa
    13081 cagtgaactg ctgatgcttg gacaatacaa acaggagaac gcgcgtctgc gcgagctgct
    13141 gggttccccg ctgcgtcagg atgagcagaa aatggtgact caggttatct ccacggttaa
    13201 cgatccttat agcgatcaag ttgttatcga taaaggtagc gttaatggcg tttatgaagg
    13261 ccagccggtc atcagcgaca aaggtgttgt tggtcaggtg gtggccgtcg ctaaactgac
    13321 cagtcgcgtg ctgctgattt gtgatgcgac ccacgcgctg ccaatccagg tgctgcgcaa
    13381 cgatatccgc gtaattgcag ccggtaacgg ttgtacggat gatttgcagc ttgagcatct
    13441 gccagcgaat acggatattc gtgttggtga tgtgctggtg acctccggtc tgggcggtcg
    13501 tttcccggaa ggctatccgg tcgcggttgt ctcttccgta aaactcgata cccagcgcgc
    13561 ttatactgtg atccaggcgc gtccgactgc aggactgcaa cgtttgcgtt atctgctgct
    13621 gctgtggggc gcggatcgta acggcgctaa cccgatgacg ccggaagagg tgcatcgtgt
    13681 tgctaatgaa cgtctgatgc agatgatgcc gcaggtattg ccttcgccag acgcgatggg
    13741 gccaaagtta cctgaaccgg caacggggat cgctcagccg actccgcagc aaccggcgac
    13801 aggaaatgca gctactgcgc ctgttgcgcc gacacagccc gctgctaatc gctctccaca
    13861 aagggctacg ccgccgcaaa gtggtgctca accgcctgcg cgtgcgccgg gagggcaata
    13921 gtggcgagct atcgtagcca gggacgctgg gtaatctggc tctctttcct cattgcgctg
    13981 ttgctgcaaa tcatgccctg gccggataac ctgattgttt tccggccaaa ctgggtgtta
    14041 cttatcttgt tgtattggat cctggccttg ccccatcgcg taaatgtggg cacaggtttt
    14101 gtgatgggtg ccatactgga tctgatcagc ggctcgatgc ttggcgtacg cgtattggcg
    14161 atgagcatca ttgcttacct ggtggcgctg aaataccagc ttttccgcaa cctcgcatta
    14221 tggcagcagg cgctggtcgt catgttgctt tcgctggtgg tggatattat tgttttctgg
    14281 gcagagtttt tagtgattaa cgtctctttc agaccggaag tgttctggag tagtgtagtc
    14341 aatggggtgc tctggccgtg gattttcttg ctgatgcgta aagtccgtca gcagtttgca
    14401 gtgcaataaa ggtttctatg acttctctgt atttagcttc cggttctccg cgtcgtcagg
    14461 agttacttgc gcaacttggc gtgacctttg aacgtattgt tacgggcatt gaggagcagc
    14521 gtcagccgca ggagagcgcg cagcagtatg ttgtgcgtct ggcacgcgag aaagcacagg
    14581 cgggtgtcgc gcaaacggca caggatctcc cggtgctggg tgcggatatt atcgttatcc
    14641 tgaacggaga agtgctcgag aaaccgcgcg acgcagagca tgcggcgcag atgttgcgca
    14701 aattatcggg tcagacccat caggtgatga cggcagtggc gttggccgac agccagcaca
    14761 ttctcgattg cctggtggtc accgatgtga ctttcagaac gttaacagac gaagacatcg
    14821 cgggctatgt cgccagcggt gaaccgttag ataaagcagg tgcatacggt attcaggggc
    14881 tgggtggctg ttttgtcagg aagataaatg gcagctatca cgccgtagtc ggcttaccgc
    14941 tggttgaaac gtatgaatta ttaagtaatt ttaacgcact gcgtgagaaa agggataaac
    15001 atgacggctg aattgttagt aaacgtaacg ccttcggaaa cgcgagtggc gtatattgat
    15061 ggcggtattc tgcaggaaat tcatattgaa cgtgaggcgc gacgcggaat agtaggcaat
    15121 atctacaagg gtcgtgtaag tcgtgtactt ccgggtatgc aggcggcttt tgtagatatt
    15181 gggctggata aagccgcgtt tcttcatgca tccgacatca tgccgcatac cgaatgtgtg
    15241 gcgggtgaag aacaaaagca attcacggtg cgcgacatct cggaactggt tcgtcagggg
    15301 caagatctga tggtgcaggt ggtgaaagat ccgcttggca ctaaaggtgc gcgcctgacc
    15361 accgatatca cgctgccttc tcgctatctg gtatttatgc caggggcttc tcacgttggg
    15421 gtttcccaac gaattgaaag cgaatcagaa cgtgaacgtc tgaaaaaagt ggtcgcagag
    15481 tattgcgacg agcagggcgg gtttatcatc cgcaccgcag cggaaggggt tggcgaggct
    15541 gaactggcct ccgatgccgc ttatctgaaa cgcgtctgga ccaaagttat ggagcgtaaa
    15601 aagcgcccgc agacccgtta tcagctgtac ggcgaactgg cgctggcgca gcgtgttctg
    15661 cgtgattttg ccgacgccga actggaccgc attcgcgttg actcacgcct gacttacgaa
    15721 gcattgctgg agtttacctc ggagtacatt cccgagatga ccagcaagct ggagcattac
    15781 accggacgcc agccgatttt cgatctcttt gatgtcgaaa acgaaatcca gcgagcgttg
    15841 gaacgcaaag tagaactgaa atccggtggt tatctgatta tcgaccagac cgaagcgatg
    15901 accaccgtgg acatcaatac cggagcgttt gtcggtcatc gcaatctgga cgacactatt
    15961 ttcaatacca atattgaagc gacgcaggct atcgctcgcc agttacggtt gcgtaatctg
    16021 ggcgggatta tcattattga tttcatcgat atgaataatg aagatcaccg ccgccgcgtg
    16081 ctgcattcgc tggagcaggc gttgagcaaa gaccgggtga aaaccagcgt taatggtttt
    16141 tcggcgctgg ggctggtgga gatgacgcgt aaacgcaccc gcgaaagcat tgagcacgta
    16201 ctgtgtaacg aatgcccaac ctgccacggt cgcggaacgg tgaaaaccgt ggaaacggta
    16261 tgctatgaaa tcatgcgcga gattgttcgt gtccaccatg cttacgactc cgaccgtttc
    16321 ctggtctatg cttctccggc agtagccgaa gccttgaaag gcgaagagtc acactcgctg
    16381 gcggaagtgg aaattttcgt tggcaaacag gttaaagtac aaattgaacc gctctataac
    16441 caggagcagt ttgacgtcgt aatgatgtaa acagatgctg ggccgccatc cggcaaaggg
    16501 tttttgagtc acatttttag cagacaagga gtgacgggtg aggcgattgc cggggatttt
    16561 actgcttact ggagccgcgc tcgttgtgat cgctgccctg ctggttagcg gcctgcgtat
    16621 tgctttaccg catcttaacg cctggcgtcc ggaaatcctc aacaaaatag aatccgcgac
    16681 tggcatgccg gtagaagcca gtcagctctc agccagctgg cagaattttg gcccgacgct
    16741 tgaagcacac gacatccgtg cagaactaaa agatggcggc gaattttcgg ttaaacgcgt
    16801 tactctggcg ctggatgtct ggcagagcct gttacatatg cgctggcagt ttcgcgacct
    16861 cactttctgg cagctgcgct ttcgcaccaa cactcctatc accagcggtg gtagcgatga
    16921 cagtctggaa gccagtcaca tcagcgatct gtttcttcgt caatttgacc atttcgatct
    16981 ccgcgacagt gaagtcagtt ttctgacgcc atccggtcag cgcgccgagc tggcgatccc
    17041 acaactcacc tggctgaacg atccacgtcg acaccgtgcg gaaggcctgg taagcctctc
    17101 cagccttacc ggacagcacg gcgtgatgca ggtgcgcatg gatttgcgcg atgatgaggg
    17161 gttgttaagc aatggtcgcg tctggctaca ggcggatgac atcgacctga agccgtggct
    17221 cggtaaatgg atgcaggaca atattgctct ggaaacggca cagttctccc ttgaaggctg
    17281 gatgacgatc gacaaaggcg atgtaaccgg cggtgacgtc tggctgaaac agggcggtgc
    17341 cagctggttg ggcgagaagc aaacgcatac gctgtcggtg gataatctga ccgcgcatat
    17401 tacgcgtgaa aatccgggct ggcagttctc tattcccgat acacggatca cgatggacgg
    17461 caaaccctgg ccgagcggag cattgacgct ggcctggata ccggaacagg acgttggcgg
    17521 caaagacaat aaacgcagtg acgaactccg gattcgcgct agtaatctgg agctggcagg
    17581 cctggagggc atacgcccgc tggccgcgaa actttcacct gcactgggtg atgtttggcg
    17641 ctccacacaa ccgagcggca agattaacac tctggcgctg gatatcccgc ttcaggcggc
    17701 agacaagacc cgttttcagg catcgtggag cgatctggcc tggaagcaat ggaaattatt
    17761 accgggtgcg gaacacttct ccgggacgct ttccggcagc gttgaaaatg gtttgcttac
    17821 cgcgtcgatg aagcaggcaa agatgcctta cgaaacggta ttccgtgcgc cactggaaat
    17881 cgccgacggc caggcaacta taagctggct gaacaatgac aaaggtttcc agctggatgg
    17941 gcgtaatatt gacgttaaag ccaaagccgt ccatgcgcgc ggcggttttc gttacctgca
    18001 acctgctaac gatgaaccct ggctgggtat tctggctggc atcagtaccg atgatggttc
    18061 acaagcctgg cgctatttcc cggaaaactt gatgggtaaa gacctggttg attatttaag
    18121 tggtgcgatt cagggcggtg aagcggataa cgcgacgctg gtttatggtg gcaatccgca
    18181 actcttcccc tataaacaca acgaaggtca gtttgaagtg ctggtgccgc tgcgcaacgc
    18241 gaagtttgcc ttccagccgg actggcctgc attaactaac cttgatattg aactggactt
    18301 tattaacgac ggtttatgga tgaaaaccga tggcgttaat ctgggcggcg tgcgcgcgag
    18361 taatctcacc gcagtgatcc ctgactactc aaaagaaaaa ctgctgattg acgctgacat
    18421 taaaggtccg ggtaaagccg ttggccctta ctttgatgag acaccgctga aagattctct
    18481 gggtgcgacc ctgcaagaac tccagctcga cggcgatgtg aatgctcgct tacatcttga
    18541 tatcccgctg aacggcgaac tggtaaccgc gaaaggtgaa gtgacgctgc gtaataacag
    18601 tctgtttatc aaaccactcg acagcatcct gaaaaatttg agcggtaaat tcagctttat
    18661 caatggcgat ctgcaaagtg aaccactgac agcaagctgg tttaatcagc cgttgaacgt
    18721 ggatttttcc accaaagaag gggcaaaagc ctaccaggtt gcggtgaatc tcaacggtaa
    18781 ctggcaaccg gcgaaaaccg gcgttctgcc tgaagcggtg aacgaagcat tgagtggcag
    18841 cgtggcgtgg gatggtaaag tgggcattga tctgccttat catgctggcg cgacctataa
    18901 cgtagagctg aacggcgatc tgaataatgt gagtagtcac ttaccttcac cgttagccaa
    18961 acctgcgggt gaaccactag cggtaaacgt taaggttgat ggcaatctca acagctttga
    19021 attaaccgga caggctggtg cggataacca tttcaatagc cgctggttgc ttggtcaaaa
    19081 gctgacgctc gatcgtgcta tttgggcggc agacagtaaa acgctcccgc cgttgccgga
    19141 acaaagtggc gttgaactta atatgccgcc gatgaatggt gccgagtggc tggccctgtt
    19201 ccagaaaggc gctgcggaga gtgtcggtgg tgcagcgagt ttcccacaac acataacgtt
    19261 acgtacgcct atgttgtcac tgggaaatca gcaatggaat aacctgagta ttgtttcgca
    19321 accgacggca aatggcaccc tggttgaggc gcaagggcgt gaaatcaacg ccacgctagc
    19381 gatgcgtaat aacgcgccgt ggctggcgaa tatcaaatat ctttattaca acccgagcgt
    19441 ggcgaaaact cgtggtgatt caacgccgtc atcacctttc ccgacaacgg agcgcattaa
    19501 cttccgtggc tggccggacg cacaaatacg atgcacagag tgctggttct gggggcaaaa
    19561 attcggtcgc attgacagtg atatcaccat ttctggcgat acgttaacgc tgaccaatgg
    19621 actgattgat actggtttct cgcggcttac tgccgatggt gaatgggtta ataatccggg
    19681 gaatgaacgt acctcgctga aaggaaaact gcgtgggcag aaaattgatg ccgccgcaga
    19741 attttttggt gtcacgacgc ccatacgcca gtcgtcattt aatgtggatt acgatttaca
    19801 ctggcgcaaa gcaccctggc agccagatga agcgacgttg aatggcatca ttcatactca
    19861 actgggtaaa ggcgaaatta ccgaaatcaa taccggacat gccgggcaat tgctgcgctt
    19921 attgagcgta gatgccctga tgcgtaagct gcgttttgat ttcagagaca cttttggcga
    19981 agggttctat tttgactcca ttcgcagcac cgcgtggatt aaagacggcg ttatgcacac
    20041 cgacgacacg ctggtggatg gcctggaggc ggatatcgcc atgaaagggt cggtaaatct
    20101 ggtacgccgc gacctgaata tggaagcggt tgtcgcacca gagatttctg cgacggtggg
    20161 cgtggctgcg gcttttgcgg ttaaccccat tgttggcgcg gcagtgtttg ccgccagtaa
    20221 agtgctgggg ccgctgtgga gcaaagtctc cattttgcgc tatcacattt cgggtccgct
    20281 ggacgatccg caaatcaacg aagtgttgcg ccaaccgcgt aaagaaaaag cgcaatgatt
    20341 tgacgagggc gcgtaattgc cccaatctca tatgataatc gttgccaaag gccaacgagc
    20401 cggaacataa ctgtaggccg gatagggcgt tcacgccgca tccggcagcc gtaaaaaatc
    20461 ctctactgca gtaactaacg agtagcaaaa acgatgagtc tgaacctggt aagtgaacaa
    20521 ttgctagcgg cgaacggcct gaaacatcag gacttgttcg cgatcctcgg tcaactggcc
    20581 gaacgtcgcc ttgattatgg cgatctctat tttcagtcga gctatcacga atcctgggtt
    20641 ttagaagacc gcattattaa agatggttct tacaacatcg atcagggcgt tggcgtgcgt
    20701 gcaatcagcg gtgaaaaaac cggatttgct tacgctgacc aaatcagcct gctggcgctg
    20761 gaacagagtg cgcaagcggc gcgcaccatc gtccgtgata gtggtgatgg caaagtacag
    20821 acgctgggcg cggtagagca tagcccgttg tatacctcgg tagatccgct gcaaagcatg
    20881 agccgtgaag agaagctgga tatcctgcgt cgcgtcgata aggttgctcg cgaagcggac
    20941 aagcgcgtac aggaagtgac tgccagcctc agtggtgtct atgaattaat tctggttgcg
    21001 gccaccgacg gcacgctagc ggcggatgtc cgtccgctgg tgcgtctttc cgtgagcgtt
    21061 ctggtcgaag aagatggcaa acgcgaacgc ggtgccagtg gcggcggcgg tcgttttggt
    21121 tatgagttct tccttgccga tcttgacggc gaagtccgtg cggatgcatg ggcaaaagaa
    21181 gcagtgcgta tggcgctggt caatctttct gccgttgctg caccagcggg caccatgccg
    21241 gtagtacttg gcgcaggttg gccgggcgtg ctgttgcatg aagcggtagg tcacggtctg
    21301 gaaggcgact tcaaccgccg tggcacttca gtatttagtg gacaggtcgg ggagctggtg
    21361 gcttcagaac tgtgtaccgt ggttgatgac ggcacgatgg tcgatcgccg tggttcggtg
    21421 gcgattgatg atgaaggtac gccaggccag tacaacgtgc tgattgagaa cggcattctg
    21481 aaaggctaca tgcaggataa actcaacgcg cgtttgatgg ggatgacgcc gactggcaac
    21541 ggtcgccgtg aatcctacgc ccatctgccc atgccgcgta tgaccaacac ctatatgctg
    21601 ccgggtaaat cgaccccgca agaaattatt gaatccgttg agtacggtat ctatgcaccg
    21661 aactttggtg gcggtcaggt ggatatcacc tccggcaaat tcgttttctc cacttcagaa
    21721 gcatatctga ttgaaaacgg taaagtaacg aagccggtga aaggcgcaac gttgattggt
    21781 tccggtatcg aaaccatgca gcagatttcg atggttggca acgacctgaa actggataac
    21841 ggcgtgggtg tctgcggtaa agaagggcaa agtttgccgg ttggcgtggg ccagccaacg
    21901 ttgaaagtcg ataacctgac tgttggcggt actgcgtaat aatttcatta ttttcagaag
    21961 gataattaat ctttctacgt gcacgaacgg tcccctcgct cctctggggt tagggtgagg
    22021 ggaacccgtt ggcacaggtt tgtagaacgt aacagtacaa tatgaattac ttctctttcc
    22081 cgcgcccgtg catctcctga aacaatttac cgacctcaac aaaataatcc gtcagcgagt
    22141 tgatcacgac ctgtaccttc agcggcagct tatctttttc ggtatataac gcataaaccg
    22201 ggcgtggatc tgactgataa cgcggcagca ggatctccag ctccccacga ttgatctcgt
    22261 tgatcaccca catcagcggc acgtaggcga tcccggcacc cgccgttagc cagcgcacca
    22321 gcgtcatcgg atcattagtc acaaatctcc cttgcgggat caggcgagtc gagatccctt
    22381 ccggtgcaat cagttcaaat tcattgtcgg gccgcacgct gtattcaagc catgaatggc
    22441 tacttaaatc ggcgggtttt tccggtatgc cgtattgtgt gagatagctt ttcgcggcgc
    22501 acaccaccat tggcatcgcg ccaagacggc gggaaaacag gctggaatcc tgcaacgcgc
    22561 cgacgcggat caccacatcc agaccgtcgg caatcaggtc gggggctgga attccggtaa
    22621 ccagattggc gctcaaacct gggtattctt tcagcatttt ggctgtcagc ccggcgagaa
    22681 cattttgtgc catagttgaa gaacagccaa tgcgtagcgt cccgatgggg gtgttattga
    22741 aggcatacag ttgctcatga acatcctgca cttcatgaag catacgacgg cagccctggt
    22801 agtaaattct accggcttcg gtcaggccaa tgctgcgtgt gctacggttt aacagcttta
    22861 cctgcaactc atcttccagt tttgacaccg tctgactgat ggacgaaacg ctcatctgta
    22921 gctgtctggc ggcggcggta aaagagccaa attcaactac tttggcaaac accgacatgc
    22981 gttttagtcg ttccattatt cactctgact taaaagtgat ttagatcaca taatatagat
    23041 aacagcataa cagttacgct aatatattaa atatcaatct acagcaatgt tgctctcgcc
    23101 cggctttcca tgcccttctc tgcggcgaca gatgctgaaa ataataacgc ctgctctctc
    23161 tatacaacca aggtcaacat gagtctgttt cccgttatcg tggtgtttgg gctgtccttc
    23221 ccaccgatat tttttgaatt gcttttatca ctggcgattt tctggctggt gcgccgggta
    23281 cttgtgccaa caggtatcta cgactttgtc tggcatccgg cgttgttcaa caccgcgctc
    23341 tattgctgct tgttttattt gatatcgcga ctgttcgttt gaggttgaag tgaaaacact
    23401 aataagaaaa ttctcccgta cggccatcac ggtcgtatta gtcattctgg ccttcatcgc
    23461 aatttttaat gcctgggtct attacaccga atccccctgg acgcgtgacg cgcgctttag
    23521 cgctgacgtc gttgcgatcg cgccggacgt ttctggactc attacccagg tgaatgttca
    23581 tgataaccag ctggtgaaaa aaggacagat actgttcacc atcgaccagc cgcgctatca
    23641 aaaggcgctt gaggaagcgc aagccgatgt tgcttattat caggtactgg cacaggagaa
    23701 acgccaggag gccggacgtc gtaaccgtct cggtgtgcag gcgatgtctc gcgaagagat
    23761 cgaccaggct aacaacgtac tacaaacggt tctgcatcag ttagcgaaag cgcaggcgac
    23821 ccgcgatctg gcaaaactgg atcttgaacg cacggtgatc cgagcgccag cagatggctg
    23881 ggtgaccaac ctcaacgtct ataccggtga gtttattact cgaggatcaa cggcggttgc
    23941 gctggtgaaa cagaactcct tctatgtact ggcctatatg gaagaaacta agctggaagg
    24001 ggtgcgtccg gggtatcgtg cagagatcac gccgcttggc agtaacaaag tgctgaaagg
    24061 gactgttgat agtgttgccg ctggggtcac caacgccagc agcacgcgtg acgacaaagg
    24121 gatggcgacc atcgactctg accttgaatg ggtgcgtttg gcgcaacgtg ttccggttcg
    24181 tattcgtctc gacaaccagc aagagaacat ctggcctgcg ggcaccactg ctacagtggt
    24241 ggtcactggc aaacaagatc gcgacgaaag ccaggattcg ttcttccgta aaatggccca
    24301 tcgcctgcgt gagtttggtt aatcacgatg ggtattttct ccattgctaa ccaacatatt
    24361 cgctttgcgg taaaactggc gaccgccatt gtactggcgc tgtttgttgg ctttcacttc
    24421 cagctggaaa cgccacgctg ggcggtactg acagcggcga ttgttgccgc cggtccggcc
    24481 tttgctgcgg gaggtgaacc gtattctggc gctattcgct atcgtggctt tttgcgcatc
    24541 atcggcacat ttattggctg tattgccgga ctggtgatca tcattgcgat gatccgcgca
    24601 ccattattga tgattctggt gtgctgtatc tgggccggtt tttgtacctg gatatcctcg
    24661 ctggtacgaa tagaaaactc gtacgcgtgg gggctggccg gttataccgc gctgatcatt
    24721 gtgatcacca ttcagccgga accattgctt acgccgcagt ttgccgtcga acgttgtagc
    24781 gagatcgtta tcggtattgt gtgtgcgatt atggcggatt tgctcttttc tccgcgatcg
    24841 atcaaacaag aagtagatcg agagctggaa agtttgctgg tcgcgcaata tcaattaatg
    24901 caactctgta tcaagcatgg cgatggtgaa gttgtcgata aagcctgggg cgacctggtg
    24961 cgacgcacca cggcactaca aggcatgcgc agcaacctga atatggaatc ttcccgctgg
    25021 gcgcgggcca atcgacgttt aaaagcgatc aatacgctat cgctgacgct gattacccaa
    25081 tcctgcgaaa cttatcttat tcagaatacg cgcccggaat tgatcactga tactttccgc
    25141 gaattttttg acacgccggt agaaaccgcg caggacgtcc acaagcagct caaacgcctg
    25201 cggagagtta tcgcctggac tggggaacgg gaaacgcctg tcaccattta tagctgggtc
    25261 gcggcggcaa cgcgttatca gcttctcaag cgcggcgtta tcagtaacac aaaaatcaac
    25321 gccaccgaag aagagatcct gcaaggcgaa ccggaagtaa aagtagagtc agccgaacgt
    25381 catcatgcaa tggttaactt ctggcgaacc acactttcct gcattctggg cacgcttttc
    25441 tggctgtgga cgggctggac ttccggcagt ggtgcaatgg tgatgattgc ggtcgtgacg
    25501 tcactggcaa tgcgtttgcc gaatccacgc atggtggcga tcgactttat ctatgggacg
    25561 ctggccgcgc tgccgttagg gctgctctac tttttggtga ttatccctaa tacccaacag
    25621 agcatgttgc tgctgtgtat tagcctggca gtgctgggat tcttcctcgg tatagaagta
    25681 cagaaacggc gactgggctc gatgggggca ctggccagca ccataaatat tatcgtgctg
    25741 gataacccga tgactttcca tttcagtcag tttctcgaca gcgcattagg acaaatcgtc
    25801 ggctgtgtgc tcgcgttcac cgttattttg ctggtgcggg ataaatcgcg cgacaggact
    25861 ggacgtgtac tgcttaatca gtttgtttct gccgctgttt ccgcgatgac taccaatgtg
    25921 gcacgtcgta aagagaacca cctcccggca ctttatcagc agctgttttt gctgatgaat
    25981 aagttcccag gggatttgcc gaaatttcgc ctggcgctga cgatgattat cgcgcaccag
    26041 cgcctgcgtg atgcgccgat cccggttaac gaggatttat cggcgtttca ccgacaaatg
    26101 cgccgcacag cagaccatgt gatatctgcc cgtagcgatg ataaacgtcg tcggtacttt
    26161 ggccagttgc tggaagaact ggaaatctac caggaaaagc tacgcatctg gcaagcgcca
    26221 ccgcaggtga cggaaccggt tcatcggctg gcggggatgc tccataagta tcaacatgcg
    26281 ttgaccgata gttaagtcaa aaccgacgcc aaaagcgtcg gttttttcat ggctatactt
    26341 agcgatgcac ggcagaactc gccgcgaaac gtgacggtgg caacagatga atatttatgc
    26401 ctttgatttt gatgagattg agagtcagga ggatttttat cgtgacttta gccaaacctt
    26461 tggtctggct aaagataagg tacgcgatct cgactcacta tgggatgtgt taatgaacga
    26521 tgtcctgccg ctaccacttg agattgaatt tgttcatctg ggagagaaaa cgcgtcgccg
    26581 ttttggcgcg ttaatattgc tgtttgatga ggcagaggaa gagctggaag ggcatttgcg
    26641 ttttaatgtt cgtcattagc gcaaaaaaaa gcccacgaac cgggggcaat atcgtcggac
    26701 aaaacgatga gggtttattt gtacagttca gccgtagcgt gccaggtgtc accgctacga
    26761 gcttcagtaa tctggtaggc cgttgcgcct ttctcttccg cttttttgtt cagcatttca
    26821 cgtatatcca ttggcgaaga cgccacacca cttacggata cggtcccgat tgcttcacga
    26881 ttttgtgctt gtgcagcatc aatggagtcg gcagcgaatg caccgaaaga gagaacagaa
    26941 agtacgctta atgcagctac agtggttttg attttcatga tttttacctc gacataatct
    27001 tttagctggg cctttgtttc gtgacccata tcacaaaatc aagtatacac taattattga
    27061 actaattaat accacactaa ctgtttcagt tgatactaag tgttaaaaat gtgaatttta
    27121 ataacaaaaa agtataaaaa acacggttat ttattattaa ttatagtatt ttcaattaga
    27181 taatgaattt tgcataatgt gcttttttta ccgtatgtat tcattttgtg aatgatgtgt
    27241 cgcggtaaaa cgcactattc gttaaatgta tcgcaggaga aagaagggga atgagaggga
    27301 taagcaacat tttccccgcc gccagaagcg acggggcaga gattaaagct cctggtcgaa
    27361 cagctctaaa atcgcttcgt acaggtcttt gacggtgaaa ccgttagcag gggtggtaaa
    27421 gatggtgtca tcgccagcga tggtgcccag aataccttct gctttgccca gtgagtccag
    27481 caggcgagca attaactgcg ccgcgccagg gctggtatga atcacgacaa ctgcatcgtt
    27541 gtagtcgata tccagcacca gattcttcaa tggactggag gtggttggta cacccagttc
    27601 agctggcagg cagtaaacca tttccatttt ggcattgcgt gtacgtacag caccaaactt
    27661 ggtcaacatc cgcgagactt tagactgatt aatattgtca aagccttgct cctgcaacgc
    27721 ggcgacgatt tcgccctggg agctaaattt ctcttcttta agtaatgctt taaatgcttt
    27781 aactagttct tcttgcttag ccgagcttcg cataagtcac ccgatatggt ggttgataca
    27841 acattattgt gcatacagat gaatttttat gcaaacagtc agccctgaag aaggctgaaa
    27901 taatgttatg aaagagcggg attttatcaa atttcgttat tgagaaacat gcctgcgtca
    27961 cggcatgcaa attctgttta aaagtaaatt aattgttatc aaattgatgt tgttttggct
    28021 gaacggtagg gtatattgtc accacctgtt ggaatgttgc gctaatgcat aagcgactgt
    28081 taattacgta agttaggttc ctgattacgg caattaaatg cataaacgct aaacttgcgt
    28141 gactacacat tcttgagatg tggtcattgt aaacggcaat tttgtggatt aaggtcgcgg
    28201 cagcggagca acatatctta gtttatcaat ataataagga gtttaggatg aaagtcgcag
    28261 tcctcggcgc tgctggcggt attggccagg cgcttgcact actgttaaaa acccaactgc
    28321 cttcaggttc agaactctct ctgtatgata tcgctccagt gactcccggt gtggccgtcg
    28381 atctgagcca tatccctact gctgtgaaaa tcaaaggttt ttctggtgaa gatgcgactc
    28441 cggcgctgga aggcgcagat gtcgttctta tctctgcagg cgtagcgcgt aaaccgggta
    28501 tggatcgttc cgacctgttt aacgttaacg ccggcatcgt gaaaaacctg gtacagcaag
    28561 ttgcgaaaaa ctgcccgaaa gcgtgcattg gtattatcac taacccggtt aacaccacag
    28621 ttgcaattgc tgctgaagtg ctgaaaaaag ccggtgttta tgacaaaaac aaactgttcg
    28681 gcgttaccac gctggatatc attcgttcca acacctttgt tgcggaactg aaaggcaaac
    28741 agccaggcga agttgaagtg ccggttattg gcggtcactc tggtgttacc attctgccgc
    28801 tgctgtcaca ggttcctggc gttagtttta ccgagcagga agtggctgat ctgaccaaac
    28861 gtatccagaa cgcgggtact gaggtggttg aagcgaaagc cggtggcggg tctgcaaccc
    28921 tgtctatggg ccaggcagct gcacgttttg gtctgtctct ggttcgtgca ctgcagggcg
    28981 aacaaggcgt tgtcgaatgt gcctacgttg aaggcgacgg tcagtacgcc cgtttcttct
    29041 ctcaaccgct gctgctgggt aaaaacggcg tggaagagcg taaatctatc ggtaccctga
    29101 gcgcatttga acagaacgcg ctggaaggta tgctggatac gctcaagaaa gatatcgccc
    29161 tgggcaaaga gttcgttaat aagtaattga ttagcgaata ataaaaaacc ggagcacaga
    29221 ctccggtttt tgttttgagc actcgactta attggttgcc ggatattcct gaatggtgac
    29281 ctgcagcgtt aactgcttat catcacgcat cactacaacc gggatcaccg aaccagggcg
    29341 aatttccgcc acctgatcca tcgtctccag agcagagatg gccggtttgt tatccaccga
    29401 aataatcaga tcgttgacct gaatacccgc attcgccgct gggccgtcag gtgacacttc
    29461 attaaccacg atcccttgca gttgatctat accaccgctc tgcgcgtgca gtggtgcgat
    29521 ctcgcgtccg ccgataccaa tgtagccgcg gatcacgcga ccatcgcgga tcagcttatc
    29581 cataattttg gttgctaact ggaaaggaat cgcaaagccg ataccttccg gcgtttcgcc
    29641 atcgttactc ttatcaaacg acagcgtgtt aatgcccatc agttctccca gcgagttcac
    29701 cagcgcgccg ccagagttac cgtggttaat ggaagcatcg gtttgtagga agttttgccg
    29761 cccggtcggg ttcagaccga ttcgacccgt ggcactaata atcccttggg taatggtctg
    29821 cccgaggttg tacgggttac cgatcgccag tactacgtcg ccaatgtgcg gtacgcgacg
    29881 tgcattaatt ggaatggtag gtaaaccgcc agtggcatta attttaagta ccgccagatc
    29941 ggttagagag tcagatccca ccagcaatgc ttcaaataca cgtccatcct gtaaggcgac
    30001 gatgatctga tcggcgtcgt tgatgacgtg tttattggtg atgatataac cgcgttgatc
    30061 catgattaca ccggatccca gggtgcggat ctcaagctgg ttgtgagagt tggtgttcaa
    30121 accacggttg taaacgttaa ccaccgctgg cgcggcgcgg cgaaccgcca gattatagct
    30181 ggcaggcgtc tcatcggtac tgtcaaattg cggagtggaa agcgggttaa ggctgcgcag
    30241 cgaaggcatg gcaaccagca gaatagcgcc gacaattaat ccaatcgcaa cggaacgtaa
    30301 gagcttcaca aacatgatgg aggcgtcatt aaaaaaggga acggcagcag cataccacga
    30361 gttaaccgga catcacacgt cagcctgatg cccggtttac gacattaacg catcagcaga
    30421 tagatgcttt cattgccgcg tacaatttgc agggcgatga tggccggttt tgccgccagc
    30481 actttacgca tttcagcaat cgagttcacc cgatcgcggt tgacgccaat gatcacatcg
    30541 tctttttgca agccagcctg agcagctggg cttcctttga caacttcatc tatcttaata
    30601 cctttgccgc catcttttag ctgaccatcg ctcaacgttg caccttccag cgctggccga
    30661 cgaagaggtg ctggtatcaa gcgtcacttc tacttccagt ggtttgccgt tacgcagcag
    30721 gccaagcttc actttcgtgc ccggctctgt agtcgcgata cgagagcgca actcagcaaa
    30781 gctattcagc ggtttgccgt tgaggctggt aataatatcg cccgctttga cgcccgcttt
    30841 cgctgagcca gaacttggca acacttcgct gacaaacgcg ccacgctgca cgtcaaggtt
    30901 gaaggctttg gcgatatcgg cactcatctc ggtgcctttg atgcctaaca aaccgcgttt
    30961 gatttcacca aagtcgataa gctgctgcgc cagtgttcgc gccatattac tggggatggc
    31021 aaatccaatc ccgacgctcc cgccgccagg cgcaaggatt gcagtgttga tgccaattaa
    31081 ctcaccgtta aggtttaata gtgcaccgcc ggagttaccg cggttaatgg aagcatctgt
    31141 ctggataaag ttttccagac cttcaagatt caacccgctg cggcctaatg cggaaacaat
    31201 gccagaggtg gcggtttgcc caaggccaaa tgggttaccg accgctacgg caaaatcacc
    31261 gacgcgcaat ttatcggagt cggcaatagc gatttgcgtt aatttgctcg ggttttgaat
    31321 ttgtaacagg gcgatatcgc tctggtcatc gctaccaatc agttttgcat caaactcgcg
    31381 cccatcattg agctgaatac tgattttctg tgcctgatta atcacatggt tgttggtcag
    31441 cacatagcct ttactggcgt tgatgatgac accggaacct aaaccttcga agggttgtgc
    31501 aggttgatcc ggtaaatcat caccaaaaaa ctttttgaat tcttccggga ttttctgtcc
    31561 ctgactggcc gttccttcca cccgtacgct caccactgcc ggaagcactt tttccagcat
    31621 tggagccaga ctggggagag gggcctgatc ggcaacctgg cctggaatcg acgcgacggc
    31681 ctgaaatgac gccgagagag ttaacccggc acttaacgct aatgcactca acagctgggt
    31741 ttgttttttc attattcctg ctctcgtacc tgaatgataa gaaaagagat tcaaaacagt
    31801 ttgatgttat tgaattttca gacggctaac aatgagacac cagattaaat gatagtcggg
    31861 gagggagaga agaggagggc gcaatggctg cgcccgaaaa ataaattagt cgcgcttcgc
    31921 gccagtacgc agcaggccgg atgcgccttc agaatagtcg cgaggcacct gcaccggtgc
    31981 ctgatcgttg ctggcttcag actctgccag acgattacgg aacgggtttg cttcagcaga
    32041 cagttccggc agcaggctgc tggagctttt tgccatgtgc tgatacagct ggcgatagtc
    32101 gtgcgccatg gtatccagta attccgcgct gcgggcaaag tggctaacca gctcttcgcg
    32161 atactcgtcc agttcagctt tattcttttc cagttcgtac tgcaacgcct gttgctggcg
    32221 tagtttacga ttaccaaaac gcatggccac agcaccaata atgatgccga cgactaaccc
    32281 aattagcgca tattcccagg tcatgaacat ctcccgttgt cttttgtttc cgtagggtgt
    32341 tggcttcagg ctccatgcct gcggctgatt atgccactat aaccgttaat tccacagaag
    32401 tggaatcccg actgcatatc gcgtagtgta gaacggcctt tttttcgtca accgtgaaca
    32461 acggcgcacc gattattcaa ggaataacaa taagatcatg caaagcgtta ccccaacatc
    32521 gcaatacctg aaggcgctca atgaaggcag ccatcaaccc gacgacgttc aaaaagaggc
    32581 cgtcagccgc ctggaaatta tttatcaaga actcatcaat agcaggccac cagcccccag
    32641 gacgagtggg ctaatggcgc gggtcggtaa gctgtggggt aaacgcgaag acacaaagca
    32701 tacgccagtg cgtggcttat atatgtgggg cggtgtagga cgcgggaaaa cctggctgat
    32761 ggaccttttc tatcaaagcc tgccgggaga gcggaaacag cgcctgcact ttcaccgttt
    32821 tatgctgcgg gtgcacgaag agctaactgc cttacagggg cagaccgatc cgctggaaat
    32881 tattgccgat cgctttaaag ccgaaactga cgtgctctgt tttgacgaat tttttgtttc
    32941 tgatattacc gacgccatgc tacttggcgg tctgatgaaa gccctgttca cccgcggtat
    33001 taccctggta gcgacgtcaa atattccgcc ggacgaactt tatcgaaatg gcctgcaacg
    33061 tgcgcgtttt ctgcctgcaa tcgatgccat taaacagcat tgtgatgtaa tgaacgtgga
    33121 cgctggtgtt gattatcgac tgcgtacact cactcaggcg catctgtggc tttcgccact
    33181 caacgatgaa acccgggcgc agatggataa actatggttg gcgctggcgg gggtgaaacg
    33241 agaaaattca ccgacgttag aaatcaacca tcggccattg gcgacaatgg gcgtcgagaa
    33301 ccagacgctg gcggtttctt ttactacgct gtgcgtcgac gcccgcagtc agcatgacta
    33361 tattgcgctc tcacgcctgt ttcacacagt aatgttgttt gatgtgccag tcatgacgcg
    33421 gttgatggag agcgaagcgc ggcgctttat tgcgctggtg gatgagtttt acgagcgcca
    33481 tgtcaaatta gtggtgagtg cagaagtgcc gctgtatgaa atttatcagg gtgagcggct
    33541 gaagtttgag ttccagcgtt gcctgtcacg tctgcaagag atgcaaagcg aagagtatct
    33601 gaagcgcgag catttggcgg gttaaaacct gccacaaatc acaaaaaggg gtcgatcttt
    33661 gaccccgact tctctataat cctgcgaccc cacgttacaa gaaagttttt ttcccaaaac
    33721 tttttgtgtg ctggcatagg ctattcgaag gggtaggttt gccggacttt gtcgtgtgaa
    33781 cctcaacaat tgaagacgtt tgggtgttca ccaacgtgta actatttatt gggtaagctt
    33841 ttaatgaaaa cttttacagc taaaccagaa accgtaaaac gcgactggta tgttgttgac
    33901 gcgaccggta aaactctggg ccgtctggct actgaactgg ctcgtcgcct gcgcggtaag
    33961 cacaaagcgg aatatactcc gcacgtagat accggtgatt acatcatcgt tctgaacgct
    34021 gacaaagttg ctgtaaccgg caacaagcgt actgacaaag tgtactatca ccacaccggc
    34081 cacatcggtg gtatcaaaca agcgaccttt gaagagatga ttgctcgccg tcctgagcgt
    34141 gtgattgaaa tcgcggttaa aggcatgttg ccaaaaggcc cgctgggtcg tgctatgttc
    34201 cgtaaactga aagtttacgc gggtaacgag cacaaccacg cggcacagca accgcaagtt
    34261 cttgacatct aatcgggatt ataggcaatg gctgaaaatc aatactacgg cactggtcgc
    34321 cgcaaaagtt ccgcagctcg cgttttcatc aaaccgggca acggtaaaat cgtaatcaac
    34381 caacgttctc tggaacagta cttcggtcgt gaaactgccc gcatggtagt tcgtcagccg
    34441 ctggaactgg tcgacatggt tgagaaactg gacctgtaca tcaccgttaa aggtggtggt
    34501 atctctggtc aggctggtgc gatccgtcac ggtatcaccc gcgctctgat ggaatacgac
    34561 gagtccctgc gttctgaact gcgtaaagct ggcttcgtta ctcgtgacgc tcgtcaggtt
    34621 gaacgtaaga aagtcggtct gcgtaaagca cgtcgtcgtc cgcagttctc caaacgttaa
    34681 ttggcttctg ctacggcaga aaacaatttg cgaaaaaacc cgcttcggcg ggttttttta
    34741 tggctaaatt ctgaatcagc gtaaaaaatg ggaagttgct ttttgctgcc gtctgaaaga
    34801 caggtaaaac aaaaaccaca tcgccaataa gggtctaagt caactatttc agactaaagc
    34861 gcatctcttt ttccccattt ccggcatcca ctcaccacaa aggtcgcaaa atctggtaaa
    34921 ctatcatcca attttctgcc caaatgtcgg gtattgctca ttttttgttt gattttcgaa
    34981 caaagagagt cgttccttat tgggtaacac aacttctgac tggccacctg gtggctggta
    35041 gcagtaaaaa ttctgactat acctggaggt tttcatggct gtcgctgcca acaaacgttc
    35101 ggtaatgacg ctgttttccg gtcctactga catctatagc catcaggtcc gcattgtgct
    35161 ggctgagaaa ggtgtaagtt tcgagatcga acacgtggaa aaggacaatc cgcctcagga
    35221 tctgattgac ctcaacccga atcagagcgt tccgaccctg gtggatcgtg agctgaccct
    35281 gtgggaatct cgcatcatta tggaatatct ggatgagcgt ttcccgcatc cgccactgat
    35341 gcctgtttac ccggtagctc gcggtgaaag ccgtctgtac atgcatcgca tcgaaaaaga
    35401 ctggtacacg ctgatgaaca ctatcatcaa cggttcagct tctgaagcag atgccgcacg
    35461 taagcaactg cgcgaagaac tgctggcgat tgcgccggtc ttcggtcaga agccgtactt
    35521 cctgagcgat gagttcagcc tggtcgattg ctaccttgct ccgctgctgt ggcgtctgcc
    35581 gcaactgggc atcgagttca gcggcccggg tgcgaaagag ctgaaaggct atatgacccg
    35641 cgtctttgag cgtgactctt tccttgcttc tttaactgaa gcagaacgtg aaatgcgtct
    35701 gggccggagt taatctgtat ggatttgtca cagctaacac cacgtcgtcc ctatctgctg
    35761 cgtgcattct atgagtggtt gctggataac cagctcacgc cgcacctggt ggtggatgtg
    35821 acgctccctg gcgtgcaggt tcctatggaa tatgcgcgtg acgggcaaat cgtactcaac
    35881 attgcgccgc gtgctgtcgg caatctggaa ctggcgaatg atgaggtgcg ctttaacgcg
    35941 cgctttggtg gcattccgcg tcaggtttct gtgccgctgg ctgccgtgct ggctatctac
    36001 gcccgtgaaa atggcgcagg cacgatgttt gagcctgaag ctgcctacga tgaagatacc
    36061 agcatcatga atgatgaaga ggcatcggca gacaacgaaa ccgttatgtc ggttattgat
    36121 ggcgacaagc cagatcacga tgatgacact catcctgacg atgaacctcc gcagccacca
    36181 cgcggtggtc gaccggcatt acgcgttgtg aagtaataca aaacaggccc aggcggcctg
    36241 ttttgtcttt ttaatgactt aataaaatag cgtgattgtg gcaatcatat tcaccacgaa
    36301 accgactgcc atggggatcg ctgtccgctt caccaccgca aaaggcgaaa cgcctgcaat
    36361 tcctgacgta acgaccacca ccgcagtaat cggcgaaacc gcgcgcgcta gcgtcgtggc
    36421 aaaatgcatc ggcataatca ttacaaccgc tggtacatgt agtccggctg cgatattcgg
    36481 aataagactg gcaaatggca taaacggcgc attgccagag cccatcacaa tggcacaaat
    36541 ggcaatgact agcgccataa taatcatcac gccaataccg cccagaccag aatgctccgc
    36601 acccctgata accgcatcaa cagtgccaat cgtcgttaag cctttcgcaa atatttcacc
    36661 cgcgaccacc agcgttacca cgttgacaaa ctgcgtaccc atgccgtcaa aaaacgcctg
    36721 cacatcgtcc attgtttcgc gcaagttatg cttgcggaag aactcaacaa acatcgtcac
    36781 cgtcaaactt agtaacatca ccaccaccag atgcagttct gactgcatca gcccgacgtg
    36841 ggcgaggaac agcgagccga gcatcaggat taacggcatc acaggtaaaa tggcgtaata
    36901 gagcggcggg acattatcga gagttttttg ctctgcctgt tcgtgattga tatttttatc
    36961 ttttttgtca aaagcgcgtt gcacgaaaaa gtgggagatc gccaccgaga taatgacgca
    37021 agaggcgacc ggaagctggt agtggaagaa gtatgtggca attttcattc ccgcgacctg
    37081 ggctgcaaaa atggagttcg tttccagaat cccccactca atggacatcg tggttgcgat
    37141 aaccgccacc gcagagaggc gacttactcc cagactcacc agcgtcggaa ataaggtgac
    37201 catcagcaac atacccagac cggaggcgct ggtaataaac tgcgccatga tttggccgat
    37261 gacgtaagtt gccgacagaa taatatacgg cgagcgaatg agttttaacg ggcggcttag
    37321 caagctcacc atcgcgcaac tggctcctat gcgctccatg tagcgggcat aaccgtccac
    37381 cgccataatc gacagcccca atccggcaat gcggttggac agcatgcgca ggatttcatt
    37441 ataaatgtcg acgatcaagt agccgctgct tttggcggga tcgagtacac caccgaaccc
    37501 taaccagact ccgcacatca tcaggaagac acctgcggca gccagcacca cctgaggttt
    37561 gtagtttttc aggatcaaat agcccatcgt aattaatacg atgacagata taattatgcc
    37621 gaacatt
//
LOCUS       DAJTSA010000030        35758 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000030 DAJTSA010000000
VERSION     DAJTSA010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.030"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..246
                     /locus_tag="OO369_001658"
     CDS             <1..246
                     /locus_tag="OO369_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5981972.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(293..1051)
                     /gene="deoR"
                     /locus_tag="OO369_001659"
     CDS             complement(293..1051)
                     /gene="deoR"
                     /locus_tag="OO369_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR5981973.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGA
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="OO369_001660"
     CDS             complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="OO369_001660"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5981974.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1990..3216
                     /gene="mdfA"
                     /locus_tag="OO369_001661"
     CDS             1990..3216
                     /gene="mdfA"
                     /locus_tag="OO369_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCR5981975.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3257..3541)
                     /locus_tag="OO369_001662"
     CDS             complement(3257..3541)
                     /locus_tag="OO369_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5981976.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="OO369_001663"
     CDS             complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="OO369_001663"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR5981977.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4442..5650)
                     /locus_tag="OO369_001664"
     CDS             complement(4442..5650)
                     /locus_tag="OO369_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5981978.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5734..6270
                     /gene="rcdA"
                     /locus_tag="OO369_001665"
     CDS             5734..6270
                     /gene="rcdA"
                     /locus_tag="OO369_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR5981979.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6445..8130)
                     /locus_tag="OO369_001666"
     CDS             complement(6445..8130)
                     /locus_tag="OO369_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR5981980.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8400..8777
                     /locus_tag="OO369_001667"
     CDS             8400..8777
                     /locus_tag="OO369_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR5981981.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="OO369_001668"
     CDS             complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="OO369_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR5981982.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9224..9511
                     /locus_tag="OO369_001669"
     CDS             9224..9511
                     /locus_tag="OO369_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR5981983.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9495..10217
                     /gene="nfsA"
                     /locus_tag="OO369_001670"
     CDS             9495..10217
                     /gene="nfsA"
                     /locus_tag="OO369_001670"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR5981984.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10278..11180
                     /gene="rimK"
                     /locus_tag="OO369_001671"
     CDS             10278..11180
                     /gene="rimK"
                     /locus_tag="OO369_001671"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR5981985.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11268..11744
                     /locus_tag="OO369_001672"
     CDS             11268..11744
                     /locus_tag="OO369_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR5981986.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12096..13208
                     /gene="potF"
                     /locus_tag="OO369_001673"
     CDS             12096..13208
                     /gene="potF"
                     /locus_tag="OO369_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR5981987.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13303..14436
                     /gene="potG"
                     /locus_tag="OO369_001674"
     CDS             13303..14436
                     /gene="potG"
                     /locus_tag="OO369_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR5981988.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14446..15399
                     /gene="potH"
                     /locus_tag="OO369_001675"
     CDS             14446..15399
                     /gene="potH"
                     /locus_tag="OO369_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR5981989.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15396..16241
                     /gene="potI"
                     /locus_tag="OO369_001676"
     CDS             15396..16241
                     /gene="potI"
                     /locus_tag="OO369_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR5981990.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16301..16789
                     /locus_tag="OO369_001677"
     CDS             16301..16789
                     /locus_tag="OO369_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR5981991.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16830..17957
                     /gene="rlmC"
                     /locus_tag="OO369_001678"
     CDS             16830..17957
                     /gene="rlmC"
                     /locus_tag="OO369_001678"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR5981992.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18132..18863)
                     /gene="artJ"
                     /locus_tag="OO369_001679"
     CDS             complement(18132..18863)
                     /gene="artJ"
                     /locus_tag="OO369_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR5981993.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19155..19823)
                     /gene="artM"
                     /locus_tag="OO369_001680"
     CDS             complement(19155..19823)
                     /gene="artM"
                     /locus_tag="OO369_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR5981994.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19823..20539)
                     /gene="artQ"
                     /locus_tag="OO369_001681"
     CDS             complement(19823..20539)
                     /gene="artQ"
                     /locus_tag="OO369_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR5981995.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20546..21277)
                     /gene="artJ"
                     /locus_tag="OO369_001682"
     CDS             complement(20546..21277)
                     /gene="artJ"
                     /locus_tag="OO369_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5981996.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21295..22023)
                     /gene="artP"
                     /locus_tag="OO369_001683"
     CDS             complement(21295..22023)
                     /gene="artP"
                     /locus_tag="OO369_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR5981997.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22241..22756)
                     /locus_tag="OO369_001684"
     CDS             complement(22241..22756)
                     /locus_tag="OO369_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5981998.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23405..25174
                     /locus_tag="OO369_001685"
     CDS             23405..25174
                     /locus_tag="OO369_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5981999.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25385..25708
                     /locus_tag="OO369_001686"
     CDS             25385..25708
                     /locus_tag="OO369_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR5982000.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25705..26535
                     /gene="amiD"
                     /locus_tag="OO369_001687"
     CDS             25705..26535
                     /gene="amiD"
                     /locus_tag="OO369_001687"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR5982001.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26532..27545)
                     /locus_tag="OO369_001688"
     CDS             complement(26532..27545)
                     /locus_tag="OO369_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5982002.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27644..29074)
                     /locus_tag="OO369_001689"
     CDS             complement(27644..29074)
                     /locus_tag="OO369_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR5982003.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29085..30086)
                     /gene="ltaE"
                     /locus_tag="OO369_001690"
     CDS             complement(29085..30086)
                     /gene="ltaE"
                     /locus_tag="OO369_001690"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR5982004.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30123..31841)
                     /gene="poxB"
                     /locus_tag="OO369_001691"
     CDS             complement(30123..31841)
                     /gene="poxB"
                     /locus_tag="OO369_001691"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR5982005.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31974..32942)
                     /gene="hcr"
                     /locus_tag="OO369_001692"
     CDS             complement(31974..32942)
                     /gene="hcr"
                     /locus_tag="OO369_001692"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR5982006.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32954..34606)
                     /gene="hcp"
                     /locus_tag="OO369_001693"
                     /gene_synonym="priS"
     CDS             complement(32954..34606)
                     /gene="hcp"
                     /locus_tag="OO369_001693"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR5982007.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34750..35649)
                     /gene="lysO"
                     /locus_tag="OO369_001694"
     CDS             complement(34750..35649)
                     /gene="lysO"
                     /locus_tag="OO369_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR5982008.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         8910 a         8839 c         9192 g         8817 t
ORIGIN      
        1 gtgaccatac cgcgtgggca gctgaaaaac ctgaaggcga gctacacgtt aacggaaccg
       61 cagcttaccg caccgctgaa aaaaggtcag gttgtcggga ccattgattt ccagcttaac
      121 ggtaaatcca ttgagcagcg tccgctgatc gtgatggaaa atgtggaaga gggcggattc
      181 tttggtagga tgtgggattt cgtgatgatg aaattccatc agtggttcgg cagctggttc
      241 tcttaatctt ctgataaccg gatggcgcga aacgtcatcc ggttatacgt cattaataca
      301 tcaacttaat acggtgcgtc tgcgcgtact tcacatactc atcttccggg caacaatcac
      361 tcaccacaat atcaaagcgt ttcaggtcac ccatgcgcgc cggacgcacc ttgccaaatt
      421 tactgtggtc gacaaccagc acatgctttt gcgccatcga catggcccag tgttttaccg
      481 gcaactcttc aagattaaaa caggtagcgc ctttactgac atgcacgccc gccgcggaat
      541 aaaaagcgat atccgggcaa aaattattca acgtttgctg aaaatcgatg ggtttgaaaa
      601 tggcgttgct ggcgtgaaat tcaccaccgc acagaaatgc gcggcaatgg ggtttctctt
      661 tcagcgccag aaaggtattt agcgaataac aaacggcggt aaaagggatt tcattatcaa
      721 tcgcttcaat aatccacggc gtggtggcgc cacagtcaaa aaagagggtc tgatcgggtt
      781 ctaccagtgt cgcagccagt tttgccgccc ggcgtttttc ttccaccagg cgggattttt
      841 gatcgcttaa caggtaatgg ctggcactgc gcggttccag aacaatatag ccgccgagca
      901 aaacgacggg cgcactgtgg ttgttcagat cgcgacgaat cgtcatctcc gaaaccccaa
      961 gcagggcggc ggcgtcttta agatgtaact tatcgctgcg ttttaattct tgcagcagct
     1021 gcccgatacg ctcttcgcga cgtgtttcca taatccctct gaatagttat tgaagcgagc
     1081 cgctcaatac tacacttttt agcagagatc agtcacgcac ccagcctttg cggatcggta
     1141 atgcaaaaca gacgcgatac cacgattgca gacgttgata gagtttatgc cccatcgctt
     1201 gccagataat ctgggcacta aggcagccaa tcatacctgc cagcaatcca ccgagcatat
     1261 ccagcggcca gtggacgcca agataaacgc gcgaccaggc aatgacgacg gccagcacca
     1321 ttaaaagtga gccggaccac aggcgatgcc agcataaaaa tgccagtgca aaggtgaaaa
     1381 tcaccgtacc gtgatcgctc gggaatgagt catccgccgc atgatgcagg aagttatagc
     1441 cgatattttc gacaaagggt cggtcgtgcg gaaaaagatg tcccatcgtc caggacacaa
     1501 acaggctgac cgccagcgcg atagcgattt ttatcaccag ttgccgttgt gctgtaagcc
     1561 cccacaacca aagtaccgcg gccagcaacg gcaccacggt aatcaaatct ttagcaataa
     1621 aaatcgccaa cgagatcatc cacggagccg agtctggcgt cgcgttaata agagagaata
     1681 gagagagatt caaattttcc agcataactt cccgacgcaa agtgattaaa agggagccaa
     1741 tacaggcaag tcgttgagaa taaagtgcag gttaaactgg gtaaagcggc atcgtcttat
     1801 ttccctcaag cggcctgttt acggtgggtg attgtaacgg gcataggtta aataaaactt
     1861 aaagaaagcg tagctatact cgcaataatg taagaatgtg cttaaccgtg gtttcagcta
     1921 caaaattcgc tttctcgtta gctgcgcttt tattaaactc tgcgcgatta ttattggcga
     1981 agaaattgca tgcaaaataa attagcttcc ggtgccaggc ttggacgtca ggcgttactt
     2041 ttccctctct gtctggtgct ttacgaattt tcaacctata tcggcaacga tatgatccaa
     2101 cccggtatgt tggccgtggt ggaacaatat caggcgggca ttgattgggt tcctacttcg
     2161 atgaccgcgt atctggcggg cgggatgttt ttacaatggc tcctggggcc gctgtcggat
     2221 cgtattggtc gccgtccggt gatgctggcg ggtgtggtgt ggtttatcgt cacctgtctg
     2281 gcaatattac tggcgcaaaa cattgaacaa ttcaccctgt tgcgcttctt gcagggcata
     2341 agcttctgtt tcattggcgc tgtgggatac gccgcaattc aggaatcctt cgaagaggcg
     2401 gtttgtatca agatcaccgc gctgatggcg aacgtggcgc tgattgctcc gctacttggt
     2461 ccgctggtgg gcgcggcgtg gatccatgtg ctgccttggg aggggatgtt tgttttgttt
     2521 gccgcattgg cagcgatctc ctttttcggt ctgcaacgag ctatgcctga aaccgccatg
     2581 cgtataggcg agaaactgtc actgaaagaa ctcggtcgtg actataagct ggtgctgaag
     2641 aacggccgct ttgtggcggg ggcgctggga ttcgttagcc tgccattgct ggcgtggatc
     2701 gcccagtcgc cgattatcat cattaccggg gagcagttga gcagctatga atatggcttg
     2761 ctgcaagtgc ctattttcgg ggcgttaatt gcgggtaact tgctgttagc gcgtctgacc
     2821 tcgcgccgca ccgtacgttc gctgattatt atgggcggct ggccgattat gattggtctg
     2881 ttggtcgctg ctgcggcaac ggttatctca tcgcacgcgt atttatggat gaccgccggg
     2941 ttaagtattt atgctttcgg tattggtctg gcgaatgcgg gactggtgcg attaaccctg
     3001 tttgccagcg atatgagtaa aggtacggtt tctgcggcga tgggaatgct gcaaatgctg
     3061 atctttaccg tcggtattga aatcagcaaa catgcctggc tgaacggggg caacggactg
     3121 tttaatctct tcaaccttgt caacggaatt ttgtggctgt cgctgatggt tatcttttta
     3181 aaagataaac agatgggaaa ttctcacgaa gggtaaaaaa atgcctgatt gatttgtgcg
     3241 atcaggcatt ctcgaattaa tggtgatggt cgtcaatctg gtgttcgata accatccctt
     3301 cacctacgct ggcaagatgg cgaacataag gatgcgggcg gtaagccgga gctggcgcag
     3361 gagccacata gacggtttgc ggcacagtcg cgacgctgac cgcttgtgga acgctgactg
     3421 aatcaggaac cacaaccact ttgtatccac tcggcacatc gacggtgaca ctttgcgcca
     3481 tcgccacacc agtaaagccc attaaaagcg cgcccaacag tagacaattt ttcataatca
     3541 tttcgcctga aataatgcag cgggttaatt tccgcgaatt atgcagcctt cattcaggca
     3601 ttgtgttgcc gggcgtgctg ccgtttttat tggtcaaatg gcgcttcgtg tttaagaact
     3661 ttatcgatca catccagtac gccttcacgg ttattgtagc ctgcccggta ttttgccgct
     3721 gcgacgactg cgctgccggc attttccatt gcaaaactaa agcctgcctg acgcagcatc
     3781 tcaatatcgt taccgccatc gccaaagacc accacttcgc tgtcgtctat tccccataat
     3841 ttctgcagtt ggcgaaggcc attggcttta tgtacgccgg ggataatcag atcgatgctg
     3901 ccgttgccgg tgtggactgg caccataata tcgccgatgg cctcatgtaa tgctttttgt
     3961 acttgtggaa tcagttcatc ggaaagattc aggccaaact taaagaaaat atcttctaag
     4021 ttgtcaaagt tatcgacgta ttccagacgg tgataataca tttccgccac cgttttcatg
     4081 gcatcgtcat attttttgag tgtataggcg ctattttttc cgcaggcaat aatttccact
     4141 tccgggcgcg tcagcaaatg ttccacgaca gtagcaaacg catccttcga cagctcgcca
     4201 ttaaaaacat ctttgccttc actcattacc cagccgccgt tttcagccac aaaggcaatt
     4261 tcattcgcaa tttcagggaa gaaagagatc aactgataat attgattccc gctggcgacc
     4321 acaaagcgaa tgccttgtgc tttcatttgc tgatactgag ccataaaccg ctcacggtta
     4381 taggtttttt gatcgcttaa gaaagtacca tccatgtcta ccgcaattaa tttaatgctc
     4441 atcaactatt ctccatcgcc gtctgcgttt tggtatcggg tttggcgacg gctttcgcga
     4501 caatagccgc gagaataacc agcgccagta caaccagcat tgcactacgt aatccataat
     4561 gttcgccgag atagcccagc agcggcggcc cgacgaggaa agccagataa ccggtcgttg
     4621 ctaccacact gacgcgggtt ggtgcatcgg ggccggtatc gctggcggca gaaatggtca
     4681 gcgggaagcc cagcgaggca cccagtcccc aaagtacaac agacactcct gcgacccagg
     4741 cgctatcgac aaaaataatc agcccaatac ccaacgcccc cattagtgca ctggcccgaa
     4801 ccacggcaac gcgactgtaa ctgtcgatga accaaccgcc ggtgaagcgt ccaacggtca
     4861 tccccagggt aaaaccggca taaatcagtg agccggaagt ggggctaaaa ccgtgaccat
     4921 caaccattaa taagggtaac cagtcgttgg cagaaccttc ggcaaaggcc atcgccagca
     4981 ctacaacacc aatcaacagc aactggatat cgcgataaaa aggaatgcct ttttcgccat
     5041 gctgggtgcc atcggcagca tttttgcccg taccgtcagg gattgcctga atggcgatat
     5101 aaataggcgc gatacctacc agcgccgcca ataaaatgtg caccgttgcc ggaacgccaa
     5161 aggccgtcag tgccatcccg acaccagcgc ctgccagcgt gcccaggcta taaaaaccgt
     5221 gcatcatcgg caaaaccgtt ttattcattt ctcgctcaac ggcggcacct tcaacgttta
     5281 tcgccacttc cgcagaacca aaacttgccc caaagacgcc gagaccaacg gcaaagagca
     5341 ggggcgatgt cagccagagt gccagactta atatcatcat cccgatcaat gcgcaggaca
     5401 tcgtgaccag gatgacatta cgtgtcccaa agcgtttcac taaccacgcc gagcagagaa
     5461 taccgctcat cgaaccgatc gacagaccaa agagaacacc gcccatttca gcgatcgaga
     5521 cagagagaat atcgcggata gcaggcgtac gggttgccca ggacgccatt aacaggcctg
     5581 gcaaaaagaa gaacataaac agcgcccagg ttcggcgttt caatgcatta cgtgaagaat
     5641 ttacggtcat agatcacgtc aaaataagaa gagggaagac aacattagca aggttgtgta
     5701 catttgtaca caattgcaga cagaggaaat gacatgcgtc gcgctaacga tccgcaacgg
     5761 cgagaaaaaa ttatccaggc cacgctggag gcggtgaaac tttacggaat acatgctgtt
     5821 acgcaccgca aaattgctac ccttgccggg gtaccgttgg gatcgatgac ctactatttt
     5881 tcaggaattg atgagttgtt gctggaggcg ttcagcagtt ttactgagat tatgtcccgg
     5941 caatatcagg cattttttag cgatgttagt gatgctcaag gcgcatgcca ggctatcacc
     6001 gatatgatct acagctcaca ggttgcaacg ccggataata tggagctgat gtaccagctc
     6061 tacgcgctgg ctagccgaaa accgctatta aaaacggtaa tgcaaaactg gatgcagcgc
     6121 agtcagcaaa cgctcgaaca atggtttgaa cccggaaccg cccgcgcgct tgatgcgttt
     6181 attgagggga tgacgctgca ttttgtcacc gaccgtaagc cgctatcgcg cgaggagatt
     6241 ttgaggatgg ttgagagggt tgcagggtag tagataaatt ttagataaca aaaaacccat
     6301 caaccttgaa ccgaaatggc ggggttgatg ggctccacaa aatggggaca tcaaagaaaa
     6361 gcagtggcac taattaagac tgatgccctg cggaaaagtt ctgcggttgt gcaaaaaaat
     6421 ttcattttca gggcaacttc agttttatcc taatcctggc cataccatga cgatgattgt
     6481 ccctgccagc gtcagcagga cgttggcgat tgcgtaggtg cccgcatagc ccagcgcagg
     6541 gatgttactg cgagctgtat cactgatgat ctccattgcc ggcgcgcagg tgcgtgcgcc
     6601 catcattgcg ccgaacaaca gtgcgcggtt cattcgcaat acataagcac cgaacaagaa
     6661 acagataacc acgggcacaa gactgacaat taatccggca atcaacatct gaccgccaat
     6721 cgcgcccagg ccgttattaa taccgctacc ggcgctcaga ccaacgcctg ccataaacac
     6781 catcaagccg aactctttca ccatgcttaa tgcaccttgc ggaatgtaac cgaaggtcgg
     6841 gtggttagca cgcataaagc ccagcataat tccggcgaat aacaacccgg cagcgttccc
     6901 catgccgaaa ctgaatgtgc tgaactggaa ggtgatcatc ccgatcatca gcccaataac
     6961 aaagaaggcg caaaatgcca gcaggtcagt gacctggctg tgaatcgaga taaagccgat
     7021 gcgatcggcg atggttttta cgcgacgggc atcaccgctg acttgtaaaa cgtcaccttt
     7081 gttaagcacg acgttgtcat ctatcggcat ctcaatctgg ctacgaatga cgcggttaag
     7141 gaagcaaccg tgatcggtca acttcagttg tgcgagacgt ttacctacag cgttatggtt
     7201 tttaacgacc acttcttcag tgacgatacg catgtcgaga aggtcacgat cgaaaacttc
     7261 tttaccgtta cggaagctgg gatcgagtcg ggcatgggcg tcgggatagc ctaccaacgc
     7321 tatttcatcg cccatttgta gcacggcatc accgtctgga tttgccagaa tcccgttacg
     7381 tcgaatacgt tcaatgtagc agccggtttg tcgataaata cccagttcac gcagattttt
     7441 gccgtcggtc caggccacca gctccgggcc gacgcgatag gcgcggatca ccggtaaata
     7501 aaccttacgg ttggcatcag tgtccaggcc acgttcgcgg gcgatttgct gggcgctggt
     7561 ctgtaagtcc tgatgctgca atttcggcaa gtaacgcgca ccaacaatca aactcaccag
     7621 accgattaaa taggttaagg catacccgag gctcagatta tccagtgcca gtgagagctg
     7681 cctgctttcc atgccggaat gatgcagtgt atcgccagca ccgaccagaa ccggtgtcga
     7741 cgtcatagag cctgctaaca taccggccgt caggccaata tcccagccaa acagcttacc
     7801 taaccctaag gcgatcacca gcgcactgcc aaccatcacc agtgctaaca ttaggtaatt
     7861 tttcccatcg cgaaaaaaaa tggaaaaaaa gttcggtccg gcttcgaccc cgacgcagaa
     7921 aataaacagc ataaagccaa gattaagcgc atcggtgtta atgctgaaat gttgttggcc
     7981 taataacagc gatacgacta aaacgccaat ggaattaccc agttggatcg aaccaagtcg
     8041 taactttccg agacatagcc caagcgcgag gaccacaaat aataacagaa tgtaattccc
     8101 atttaacaat tcggcgacgt ttatattcac ggaggctaac ttcttgttta ctagtaagct
     8161 gttgaaagaa atgctaattt acgataatgt gttttaccag aattcagggc gcagattcat
     8221 tcagcgcacc taaacgatag taaagtaaca atatatttta ctagtgtaat cacattaggt
     8281 atcaacggct atatgaattg cgttggccta tattagcatg gaatgcgaag cggctttatc
     8341 ttactgaacg ccacactggc gaaaaatgtg ttcgatagac gcagtgtcag gaggaacgag
     8401 tgaaacataa acaacgttgg gcgggggcaa tctgctgttt tgtcctcttc attgtggtgt
     8461 gcctttttct ggcgacgcac atgaaaggcg cttttcgggc tgccgggcat cctgaaatcg
     8521 gcttgctgtt tttcattctt cctggagcag ttgccagttt cttttcacag cgtagagaag
     8581 tcctgaaacc tctatttggc gcaatgctgg cggcaccctg ttcaatgctc attatgcggc
     8641 tgtttttttc accgacgcgc tcattctggc aagagctggc atggttacta agcgcggtgt
     8701 tctggtgtgc gctgggggca ctgtgtttct tatttatcag tagtttgttt aaaccacagc
     8761 acagaaaaaa tcagtaaagc cctcaacgcg agggcttgtc agacgatcag gcgtccagat
     8821 tttctttcac ccatgcagca aaatcggtat agccgccgat atgttgctga tcgacaaaaa
     8881 tctgcggcac ggtttctacg ggtttacctg ccttttgttg tagatcttct ttagtgatcc
     8941 cttccgcacg aatatctaca tactgatact gaaaatcatc gcgttcattg ctcaatttct
     9001 cagccagatc ttttgcacgc acacagtaag ggcaacccga acgaccaaaa ataacggttt
     9061 gcattatttc tctcctcata gatttatgcc tgtaatgatc acgctaaaat gtattcgctg
     9121 aaagtaggtt taacctgttg cattaattgc taaaagctat aactgttaaa cacaatacag
     9181 tgaaaagttt tagactgaag gctcactttg cagagggaag cgtatgcgcg cgatcggtaa
     9241 attgcctaaa ggcgtgttga tactggaatt tatcggaatg atgctactgg cggtggcgct
     9301 gctgtcggta agcgactccc tgtcgctgcc tgagccattt tctcggccag aagtgcagat
     9361 tctgatgatt tttctcggtg ttttgctcat gcttcccgct gcggtggtgg ttattcttca
     9421 ggtggcaaaa cgtcttgccc cacagctgat gaactgtcca ccgcaatatt cacgttcaga
     9481 aagagaaaaa gataatgacg ccaaccattg aacttatttg tggccatcgc tccattcgcc
     9541 atttcactga tgaacccatt tccgaagcgc agtgtgaggc gattattaac agcgcccgtg
     9601 cgacgtccag ttccagtttt ttgcagtgta gtagcattat tcgcattacc gacaaagcat
     9661 tacgtgaaga actggtgacg ctgaccggcg ggcaaaaaca cgtagcgcaa gcggcggagt
     9721 tctgggtgtt ctgtgccgac tttaaccgcc atttacagat ctgtccggat gctcagctcg
     9781 gcctggcgga acaactgttg ctcggtgtcg ttgatacggc aatgatggcg cagaatgcat
     9841 taaccgcagc ggaatcgctg ggattgggcg gggtatatat cggcggcctg cgcaataata
     9901 ttgaagcggt gacgaaactg ctgaaattac cgcagcatgt tctgccgctg tttgggctgt
     9961 gccttggctg gcctgcggat aatccggatc tcaagccgcg tttaccggcc tccattttgg
    10021 tgcatgaaaa cagctatcaa ccgctggata aagacgcact ggcgcagtat gacgagcaac
    10081 tggcggaata ttacctcacc cgtggcagca ataatcgctg ggatacctgg agcgatcata
    10141 tccgccgaac aatcattaaa gaaagccgcc catttattct ggattatttg cacaaacagg
    10201 gttgggcgac gcgctaaaac cgccacgtcg atgtatgata cgcgggcttt tgaccaggtc
    10261 tgacagagag gtgcagggtg aaaattgcca tattgtcccg ggatggaacg ctctattcgt
    10321 gtaagcggct gcgtgaagcc gctatacagc gcggtcacct ggttgaaatt cttgatccgc
    10381 tttcttgcta catgaacata aatcctgcgg cgtcttctat ttactacaaa ggccgcaagt
    10441 taccccattt tgacgcagtg atcccgcgta ttggcaccgc cattaccttt tatgggacgg
    10501 cggcactgcg ccagttcgag atgctgggga gctatccgct caatgagtcg gtcgccattg
    10561 cccgggcgcg tgacaaattg cgttccatgc aactgctggc gcgtcagggc atcgacctgc
    10621 ctgtcacggg cattgcgcat tcgccggatg ataccagcga tttaatcgac atggtcggtg
    10681 gtgcgccgct ggtggtcaag ttggttgaag gcacgcaggg aattggcgtc gtgctggcgg
    10741 agactcgtca ggcggcggaa agcgtgattg acgctttccg cggtctgaac gcgcatattc
    10801 tggtgcagga atatatcaaa gaggcgcaag ggtgcgatat ccgctgtctg gttgttggtg
    10861 atgaagtggt cgctgcgatt gaacggcggg cgaaagaggg ctattttcgt tccaatttgc
    10921 atcgtggcgg cgcggcaagc gtcgccagta tcacaccaca ggagcgtgaa atcgcgataa
    10981 aagccgcgcg aacgatggcg ctggacgttg ctggtgtgga tattctgcgt gctaatcgcg
    11041 ggccgttggt gatggaagtg aatgcgtcgc cggggctgga aggaatagaa aaaaccaccg
    11101 gtatcgatat cgcgggtaaa atgatccgct ggatcgaacg ccacgctacg acagaatatt
    11161 gcctgaaaac gggtggttag tcgcaatcac attactgatc atggttttgc ctgcgctttt
    11221 tgcgtaagct gtgccggtct ttttatcgaa agaggttgta caaaattatg acatcgctgg
    11281 tcgttcctgg tctggatacg ctgcgtcaat ggctcgatga cctggggatg agtttttttg
    11341 aatgtgataa ctgtcaggct ctgcatctgc cccatatgca gaatttcgac ggtgtctttg
    11401 atgccaaaat cgatctgatc gataacacga tcctgttttc tgccatggcg gaagtccgac
    11461 cttcagccgt attgccgctg gcggcggatt tatctgccat caatgccagt tcgctgaccg
    11521 tgaaagcatt tcttgatatg caggatgata atctgccaaa gctggtggtt tgccagtctt
    11581 tatccgttat gcagggcgta acctatgagc agtttgcatg gttcgtgcgt cagagcgaag
    11641 agcagatttc gatggtcatt cttgaagcta atgcccatca actgctgtta ccgactgacg
    11701 atgaagggca aaacaacgtt accgaaaact attttctcca ctgataattc ctttcgagca
    11761 cgcagtcgct gccgcagtga ctgcgcgctg caaaattatc tgctgttttt aaccttttct
    11821 taaagattat ttcacttctc ttgtgtcgat ttggctttat cacatagagc aaatacgcat
    11881 aaaaatttgt taaataccgt tttttaatcc gagctatagt ctcaaaccct ggctaaagtt
    11941 attcttgcga tgcttctata tagcgagcag tgctggccgg gagagagttc tcttttctta
    12001 caccgcgccg ataaaaaaat atgcacgttt attgcatatc tttcagtgtg acaacttttg
    12061 ttcgtttgtt aacgaacttt cagaaggaaa gagatgtgac cgccttaaat aaaaaatggc
    12121 tatcgggtct ggttgcgggt gctctgatgg ccgtctctgt cggcacgctc gcggctgaac
    12181 aaaaaacact ccacatttat aactggtctg attatatcgc ccctgacacg gtggccaatt
    12241 ttgaaaaaga aaccggtatt aaagtcgtct acgatgtttt cgactctaac gaagtactgg
    12301 aaggcaaatt aatggcgggt agcaccggct ttgacttggt ggttccatct gccagctttc
    12361 tagaacgcca gttgactgcg ggagttttcc agccgctgga caaaagcaaa ttgccggagt
    12421 ggaagaatct cgacccggag ctgctgaagc tggtcgccaa acacgatccc gacaataaat
    12481 ttgctatgcc ctatatgtgg gcgacgaccg ggattggcta taacgttgat aaagttaaag
    12541 ctgtgctggg cgaaaacgcg cccgtcgata gctgggactt gatcctcaaa cctgaaaatc
    12601 tggaaaaact gaaaagctgc ggtgtctctt tcctggatgc gccagaagaa gtttttgcta
    12661 ccgtgttgaa ttatctcggc aaagatccca acagcactaa agcggatgat tacaccggac
    12721 cggcaaccga tctgctgtta aagctgcgcc cgaacattcg ttatttccat tcatctcaat
    12781 acattaacga cctggcaaac ggcgatattt gcgtcgctat cggctgggca ggtgatgtct
    12841 ggcaggcgtc aaaccgcgcg aaggaagcga agaatggagt gaatgtctcg ttctcgattc
    12901 caaaagaagg ggcgatggcg ttctttgatg tattcgccat gccagcggat gccaaaaaca
    12961 aagacgaagc ctatcagttc ctgaattacc tgctgcgccc ggatgtagta gcgcatattt
    13021 ccgaccatgt gttctatgtc aacgccaata aagcagccac gccgctggtg agtgcggaag
    13081 tccgtgagaa cccgggtatt tatccgcctg cggatgttcg tgcgaagctg ttcactctga
    13141 aagtgcagga cccgaaaatc gaccgtgtgc gcacccgcgc gtggaccaaa gtgaagagcg
    13201 gaaaataatc cgcagcctta gatgccgggc gggcgcacca cacccgccgg caattcgcac
    13261 catcatggtg cgcttgcaca cattcaatgc cggagagcag ccgtgaatga cgctatccct
    13321 cgcccgcagg cgaaaacccg taaggcgctg acgccgctat tagaaatccg caacctcacc
    13381 aaatcctacg atggtcaaca tgcggtggat gatgtcagcc tgaccatcta caaaggtgaa
    13441 atcttcgcgc tgctgggcgc atccggctgt ggcaagtcca cgctgctgcg tatgctggca
    13501 ggtttcgaac aaccttctgc cggacagata atgcttgatg gcgtcgattt gtcacaggtt
    13561 ccgccttacc tgcgccccat caatatgatg tttcagtctt acgcgctgtt tccgcatatg
    13621 accgtggaac agaacatcgc ttttggcctg aaacaggaca aactaccgaa agcggaaatt
    13681 tccagtcggg tcaatgagat gctcggcctg gtgcacatgc aggagttcgc caaacgtaaa
    13741 ccgcatcagc tttctggtgg tcagcgacag cgtgtggccc tggcccgaag ccttgccaag
    13801 cgcccgaaac tattactgct cgacgagccg atgggcgcgc tggataaaaa gctgcgtgac
    13861 cggatgcagc ttgaagtggt ggatattctg gagcgcgtcg gtgtgacttg tgtgatggtc
    13921 acccacgatc aggaagaggc gatgaccatg gcggggcgca tcgccattat gaatcgtggg
    13981 aaatttgtcc agattggcga accggaagag atctacgagc atccgactac ccgctatagc
    14041 gccgaattta ttggctcggt aaacgtcttt gaaggcgtac tcaaagagcg tcaggaagat
    14101 ggcttggtgc ttgattcgcc ggggctggtg catccactga aagtcgacgc ggatgcctcg
    14161 gtggtcgata acgtgccggt acatgtggcg ctgcgcccgg aaaaaatcat gctttgcgaa
    14221 gagccgcccg ccaatggttg taacttcgcg gtgggggagg tgatacacat tgcctatctc
    14281 ggcgatcttt cggtgtatca cgttcgtctg aaaagtgggc agatgattag tgcccagcta
    14341 caaaacgccc atcgtcatcg taaagggtta ccgacctggg gcgacgaagt gcgtttgtgc
    14401 tgggaagtgg acagctgtgt ggtgctgacg gtttaaggag caaagatgaa tacacttgaa
    14461 cctgctgccc agtcgaaacc gccgggcgga tttaagctgt ggttgtcgca gctgcaaatg
    14521 aagcatgggc gcaaactggt cattgcgttg ccatatatct ggttgatctt gctgtttctg
    14581 ctgccatttc tgattgtctt taaaataagc ctggcggaga tggcgcgcgc tattccacct
    14641 tataccgaac tgatggagtg ggctgacggg caactgtcca tcactcttaa tctcggtaat
    14701 tttctgcaac tgaccgacga tccgctctat ttcgatgctt atctccagtc gctacaggtg
    14761 gctgcgattt cgacattttg ctgtttactg atcggctatc cgctggcgtg ggcggtggcg
    14821 cacagtaagc cttcgacccg taatatttta ttactactgg tgatcctgcc gtcgtggacc
    14881 tcgtttctga tccgcgttta tgcttggatg ggaatattaa aaaacaacgg tgtgctgaat
    14941 aattttctgc tgtggctggg ggttatcgat caaccgctga ccattcttca taccaatctg
    15001 gcagtttata ttggcattgt ttacgcctac gtgccgttta tggtgctgcc gatttatacc
    15061 gcgttgattc gtattgatta ttcgctggtg gaagcagcgc tggatctcgg tgcacgaccg
    15121 ctgaaaacgt tctttactgt gatcgtgccg ctgactaaag gtgggattat tgccggatcg
    15181 atgctggtgt ttatcccggc tgtgggcgag tttgtgatcc cggaactgct cggtggcccg
    15241 gacagcatca tgatcgggcg cgtgctatgg caagagtttt tcaataaccg cgactggccg
    15301 gtggcctcgg cggtagcgat catcatgttg ctgctgctaa ttgtgccgat aatgtggttc
    15361 cacaaacacc agcaaaaaag cgtgggagaa cacggatgaa taatttaccg gtagttcgtt
    15421 cgccctggcg gattgtgatt ttgctgctgg gctttacctt tctctacgcg ccaatgctga
    15481 tgctggttat ctattcgttt aacagctcga agctggtgac ggtgtggggc ggctggtcaa
    15541 cgcgctggta tggtgagtta ttgcgcgatg acgcgatgat gagtgcggtt ggttcaagcc
    15601 tgacaattgc tgcctgtgcg gcaacggcgg cggcgatcct cgggactatt gcggcggtgg
    15661 tgctggtgcg ctttggcagg tttcgcggat caaatggctt tgcctttatg atcaccgcgc
    15721 cgctagtaat gccagatgtc atcacgggct tgtcgctgtt gttattattc gtcgcgcttg
    15781 ctcatgccat tggctggcct gcggaccgcg gtatgcttac catctggctg gcgcatgtca
    15841 ctttctgtac ggcttatgtg gcggtcgtta tttcgtcgcg tctgcgggaa ctggatcgct
    15901 cgatagaaga agccgcgatg gatctcggtg cgacgccgct gaaagtgttt ttcgtcatta
    15961 cgctaccgat gatcatgccc gcgatcattt ctggctggtt actggctttt actttgtcgc
    16021 ttgatgatct ggtgatcgcc agctttgttt ccgggccggg agccaccacg ttaccgatgc
    16081 tggtcttttc cagcgtgcgg atgggggtga atccggaaat caacgccctg gcaacattga
    16141 tcctcggcgc ggtcggaatt gtcggattta tcgcctggta tctgatggcg cgcgcagaaa
    16201 aacagcggat acgcgacatc caacgtgcaa gacgtggctg aagacactaa aatttgccaa
    16261 cctggctaca taatgccgcg catgtcgcgg cattgttttc atggaagacg aaacgttggg
    16321 attttttaag aaaacatctt catctcatgc tcgcctgaat gtgcctgcgc tggtgcaggt
    16381 ggcggcgctc gccattatta tgatccgtgg cctcgacgtg ctgatgattt tcaatacgct
    16441 gggcgtgcgc ggtattggcg agttcattca tcgcagcgta caaacctgga gtttaacgct
    16501 ggtcttttta agcagtctgg tgctggtttt cattgagatc tggtgtgcgt tttcactggt
    16561 gaaagggcgt cgctgggcgc gctggctata tctgctgaca caaatcaccg ccgcaagtta
    16621 cttgtgggcg gcttcgctgg ggtacggtta tccggagctg ttcagcattc ccggtgaatc
    16681 aaaacgtgaa atcttccata gcctgatgct gcagaagctg ccggatatgc tcatcctgat
    16741 gctgctgttc gttccctcga ccagtcggcg gttcttccag ttgcaataat gtgtataatc
    16801 gtcgcccctg atgatgtgaa ggtcaatgta tgcagtgcgc actttacgac gcgggtcgct
    16861 gtcgttcctg tcagtggata acgcagccga ttccagagca actctccgct aaaaccgccg
    16921 atcttaaaaa tctgctcgcc gattttccgg ttgaggaatg gtgcgcgccg gtgtcaggcc
    16981 cggaacaagg gtttcgtaat aaagccaaaa tggtggtgag tggtagcgtt gaaaaaacac
    17041 tgctcggtat gctgcatcgc gatggcacgc cggaagacct ttgtgactgc ccgctttatc
    17101 cagcctcatt tgcgcccgtt tttgcggcgc taaaaccgtt tatcgcccga gcggggttaa
    17161 cgccctacaa cgtggcgcgt aagcgtggcg aactgaaata cattctgctg actgaaagcc
    17221 agagtgatgg cggcatgatg ctgcgctttg tactgcgttc tgataccaag ctggcgcaac
    17281 tgcgtaaggc gctgccgtgg ttacacgaac aactaccgca gctgaaagtt attaccgtca
    17341 atattcagcc ggtacatatg gcgattatgg aaggggagac ggagatctac ctgaccgaac
    17401 aacaggcact ggcggagcgt tttaatgacg taccgctgtg gatccgtccg caaagtttct
    17461 tccagactaa tccggcggtc gccagccagt tgtacgccac cgcgcgcgac tgggtacgac
    17521 agctgccggt taaacatatg tgggatctgt tctgcggtgt ggggggcttt ggtttacact
    17581 gcgcgacgcc tgacatgcag ttaaccggga tcgaaattgc gccagaggcc attgcctgtg
    17641 caaagcagtc agccgctgaa ctgggcttaa ctcgtttaca atttcaggcg ctggactcca
    17701 ctcagtttgc cactgctcag ggggaggtgc cggagctggt gctggttaac ccgccgcgcc
    17761 gcggcattgg taaaccgctg tgtgattatc tttcaacgat ggcaccgcgt tttattatct
    17821 actccagctg taacgcccaa accatggcga aagatatccg cgaactgcca ggttaccgca
    17881 tcgaacgggt acagcttttt gatatgttcc cgcacaccgc gcactatgaa gtgttgacgc
    17941 tgctggtgaa gcaataaaaa agccacaggt gcggcttcag attgctgatc aggtgcgctt
    18001 tgttcatgcc ggatgcggcg tgaacgcctt atccggccta caaaatcatg ctaaatcaat
    18061 acattgcaga gttgatgtgg gccggataag cgcagcgcat caggcaatct tacttttgtc
    18121 atcagtctca attactgcgg aaaccactgg tcattgattt tctgatatgt accgtcagct
    18181 ttaattgctg ccagcgcgtt attcagtttt tccagcagag ctttgttatc cggacgtaca
    18241 gcgataccca ggccggtgcc gaagtattgc ggatcggtca ctttttcagt cgcaacgccc
    18301 agttgcggat tggttttcag ccattcgttt accaccgcgg tgtcaccaaa taccccatca
    18361 atacgaccat ttttcagatc gataaaggca ttctgataac tgtcatagga gacagttttc
    18421 acttccgggt gctgatcctg aatatatttc tggtgcgtcg taccgttttc catcccaatg
    18481 cgtttgcctt tcaggtcggc aaacgttttg taggtatctt ttttggaaat cacgacggct
    18541 gagttttcat agtagggtgt ggtaaacgaa acctgcttgc tacgctccgg ggtgatatcc
    18601 ataccggaga ttacggcgtc atattttctg aatttcagtg acgggatcag gctgtcgaac
    18661 gcgtgattag taaaagtaca ttctgcctgc atttgtttgc acaaggcttt tgccagatcg
    18721 atatcaaagc cgacaatctc attattagca cctatagatt caaagggtgg ataggtggct
    18781 gaaacgccaa aattgatttt ctctgcggca gaagcaccga aagtaaagga agcaagtaaa
    18841 gcggcaagaa ctaacttttt catgatggaa ctcccgtctg tcaatcttat gatttttggc
    18901 cgtgtctgcg gcatgggata acaatgccat caagtgaatt tatatgcaat aaacatgatt
    18961 aaataattta aatgaaataa aaaaagacgg ataacttagt gagttgtccg tcttcattat
    19021 aagaatttat gcactatgta ggccggataa ggcgtccacg ccgcatccgg cacaggcacc
    19081 gtgctgatgt ctgatgcgac gctggcgcgt cttatcagac ctacaaaccc ccccggcgaa
    19141 tgtacgcagc tacattaatt ccgccgttcg aatgccagtg ctttgcgctc gatcagacgc
    19201 atcatcagcg tcagcaggcc gttgacgacc aggtaaataa tccctgccgc accgaacacc
    19261 attacatcgt aggtgcgtcc gtacaacaac tggctgtatc ccatcacttc catcagcgta
    19321 atggtgtaag ccagagaggt acttttgaac accagcacca cttcgttgga ataagaagag
    19381 agcgagcgtt taaaggcata cggcagcagg atcgccagcg tatctttttt gctcattccc
    19441 agggcgctac aggactgcca ctgaccttct gggatcgcac gaattgcacc gtaaaacagc
    19501 tgcgtggtat acgccgcact gttcagcgat aacgcaatca gcgcacataa ccacggttct
    19561 gacaacaaat gccacagtgc cggatactcc tgcaacgtcg gaaactggcc cggcccgtaa
    19621 taaatcagga agatctgcac cagcagcggc gtaccggtaa acagcgtgat ataaccccgc
    19681 accagccaca ccagcaccgg cgttttcagc gtcaggatga tggtaaaaat caatgccaga
    19741 atcagtgcca caatcagcga ggcaacggtt agcgtcaggc tggtgtgcag ccctttcatc
    19801 agctcgggta aatactcaaa cattagctgg gtctccgctc aaaacgtgtc gcgcgcaggt
    19861 caatgcgttt gagaatgtac tgactgagca gggtgatcac caggtaaatc gccgccgcca
    19921 caatgtacca ggtaaatggt tcctgggtac gagtagcaat gcttttggtt tgtaacatta
    19981 aatcattcac actaatcaaa ctgaccagcg cggtatcttt cagcagcacc agccactggt
    20041 taccgaggcc aggcagcgca tgacgccaca tctgcggcat caccagacgg aaaaagatag
    20101 ccgatttcga cagccccagc gcctgaccag attcccactg gcccaccggt acagctttca
    20161 acgcgccgcg cagcgtttgc gaggcatagg cggcatacag cagtgacaga gcgatgacac
    20221 cacaaaggaa cggactcacg tcgaagttct caatgtccat ctgcaccggg atctgcacga
    20281 acccaagatt gatagtgaag ccatccgaaa gcgtcagcag taactgcgag gagccaaaat
    20341 agataaacag cactaccaga atttctggca ggccacgcag aatggttacc agcgctgaac
    20401 ctgcccacgc gacaggacgc catttcgctg actcccatac cgcaaagaac atcgccagcg
    20461 ccagcccgac aatcaatgca caaacggcaa ggccgacggt catcccggcg gcgcttgcta
    20521 aaggaaaaaa ttcattcatc aggaattact tctggaacca tttgttgtag atggtttcgt
    20581 aagtgccatc tttcttcact ttttccagcg cagtgttgag tttctgctgc agttcagtgt
    20641 ttccctgacg taccgcgatg ccgaggccag taccgaaata atctttatcg gtcactttat
    20701 cgcccactgc cgccagtttt gggttatctt tcagccactc agtgaccact gcggtgtcac
    20761 caaatacgct gtcgatacgc ccgttttgca gatccagttt tgcgttctgg tagctgtcat
    20821 acggaacggt ggtgatttcc gggtgcttat ccataatgaa cttctggtgt gtcgtcccgt
    20881 tctgaacgcc gacttttttg cctttcagct gatcaacact ggtgtatttg ccttgctgac
    20941 ccacaaacag ggcagagttg tcatagtacg gggtagtaaa cagtacctgc ttttcacgct
    21001 ccggagtgat atccatgccc gccatcactg cttctacgcg acggaatttc aggcttggga
    21061 tcaggctgtc aaacgcctgg ttagagaaag tacaggttgc atcaatctct ttacacagtg
    21121 cctgcgccag gtcgacgtca aaaccaacga tctggttgtt tgcatcaatc gattcaaacg
    21181 gaggataggg ggcttcggta gcaaaacgaa tggtttcggc agctgtggcg gaaagactaa
    21241 aacctgcaat taacgcggca atcagaactt ttttcattgt tgttatcccg aatcttagtg
    21301 agagagatag tttttaaatg cttcggtttg cggctcggta aagcagctcg cgtcgccttg
    21361 ttctacgata tgaccatttt ccatatacac cactcggctg gcggttttac gcgccacttc
    21421 aacttcgtgg gtgacgatca cctgggtaat attcgtttct gccagctcac gaatgatgct
    21481 gacgatttgt gccgtaattt ccgggtccag tgcggcggtc ggttcatcga acagcagtac
    21541 ctgcggttcc atcatcaacg cacgggcaat agcaacacgc tgctgctgac caccagaaag
    21601 atgcagcggg taacgatcgc tataaggttt gagacgcaga cgttccagca gtttttctgc
    21661 acgggccagc gcctgatctt tactcaaccc cagtacacgg cagggcgctt caatcaggtt
    21721 ttgctgcacg gtcagatgcg gccacaggtt gtattgctga aacaccatgc caacgttacg
    21781 acgcaaatcg cgaatcgctt tgtcagaggg tgttttggtg aaatcgaaat ggttgcctgc
    21841 aatgttgagc gtaccggagc gcggcatctc aagcagattg agtacacgca gcagcgagct
    21901 tttacccgcg ccgctggggc caagtaacac cagcgtttcg ccctgtgggc aatccagcgt
    21961 gatatcgaac agcgcctgat gcgcgccgta gaagcaatta atgccgttta attgaatact
    22021 cattgacact cgtatactgg cagtctgata gctattgagg tcgaagatag tacctttgac
    22081 agaataatta tgcaatattt atgctttaaa agttaaaagc aaagcgcatt attcaataaa
    22141 catagcacaa aataacgggg gcggtggtcg gtgagcataa atgtcggcat tcctcacgaa
    22201 atgccggata atttacgggg tttattggtt gatcaaggcg ttagcgattc tcgatggact
    22261 gacggagcgt acccgccgtg gcatgaacgc taccgcctaa gtagcgcaca tcgtcgatga
    22321 cccaacactg gccttcctga atcattaaca cttcatcctg ccagccctgg tcaccctgtt
    22381 tgagatccac ccgcaatgga atgttacggg catcacgatt agggatagtc gatgcactgg
    22441 caacgtgggc gctgtctggc aaggtggttc gactggagaa cggatcgctg cttaacaatt
    22501 cacgatgact attatcgcgg ctggcatcgc taagttgggt tgccagtttg tcactcaaat
    22561 aggggcgcag ggcggtaatg tcattgctgc gatgttgaat gcggtagtcg tagaattgtt
    22621 gagccacact gtcaggccct ccctggacac aggaaccggt gcgggggccg ttatctttat
    22681 aagctggcgt gacggtggta caggcactca gcagcagtgc acatggaata agtagagata
    22741 gtgaacggta gcgcataatc atttccttat tagcttgctg tctgcgtcac actacgacca
    22801 aacgctatca gattatttaa tacattgttt ttaatacaat tctgaaacaa tctgaacacc
    22861 tgattgggtt tgagtccatt gctggcgacc cactcaaggt tgctcgtagc tgacttactt
    22921 aaattaccca aacaatagac agtgtaaacc atttgtgagg ttgagcgtgt tatatggctg
    22981 aaatgatcat ttaaggtaaa tatgatagtt ctgaaatggt ggtataaatc ctcctgtaga
    23041 ggttaagagc atctgcaaga agccagtttc acatttataa cgcgtgataa atgaatgcgg
    23101 ttattgtcta atcattacaa aatttttcac atgcgataag agatcctgcg attaagatga
    23161 ttgccaaaat tacgttgtat ttttgaaaat gagcgaaatt tgatagtccc agctttgact
    23221 ctcgataata ttatttatgc tacaattgtg ggtaaagaat ttaagctaat gaatttgagt
    23281 gaagaataaa ttaaattgct tcagtatttc gttaattaat ataaatacaa aaggcgttgt
    23341 gcttttataa ttataaagcg cgaggagttc ggtggattat cgtttttctt gggtgatttt
    23401 taatatgata aacaatatat ctgatcaagc atcatcattt ccaggcacac aactaaatca
    23461 aagtgataat ttcctggatt cactgaggga atttttcgcg atattaaatc ccagtcgtaa
    23521 gggtgagttg tctacatggg ataccatcta cttgcatctg atattagcaa taaatgctga
    23581 cagtgatctc ataaaaaatg atgtgttgct cgcggaaaat attccatcag cgaattacca
    23641 atttaataca ttttttagca atactttcga gattgatgtt aaaaaatatc tgggtaaatc
    23701 agaagataac gaagttgaaa taaaagcggg taatgaaaga atctctattg gaatacgtaa
    23761 tgtatcgaat ggaaaacttg aaagacaaca gtttcttttc ccactggact atgagaataa
    23821 attacaagaa caattagata agtattttac gattgaatcg cacccattat tatacaggta
    23881 taccattggt agtaagattg cgaatgtgat ttttgaaaag ttatacagtc gtattgactt
    23941 taacaaagaa caatatatat cctttataaa ggacgcattt attcattttt atgattatag
    24001 caggcgatat gctattagtg agaatatcga taaagacgct gtaacaaaca atatcgcatt
    24061 gatgagcact ttttatgaca gtgataatac gtcaggtgag gttttaaata atgattttac
    24121 agaagaagag tcatttgaaa ctgcgttaga tgtagagcat gcaatagtgc ttggatttgc
    24181 tgatgataat tttgaaacaa aacctgttca ttatcaggat ttattaacac gatttagtgc
    24241 ctttcaagat accgtgttta atctgtttcc tgaaatgcac tcttcgcatt accacgatat
    24301 ttgtagcgta agtgtggata tgacaaaggg aacgcaatgc atgattcact tgatggtgaa
    24361 tgaagaagtt ttcatgtctc tgcctgttcc agttgccacg atggtacgtg aagatgcaag
    24421 taatttagtc aatcttaaaa cactactcaa tgatggttgt ttcattaaat atagtcattt
    24481 taatgacgtg gcattaataa agcaaaacat aagtaatctc tatttatcac acactgttgt
    24541 gaatgaaagc atattgaaaa aatgttgttt tgaaaacggc agtttaggtg atgttaaaat
    24601 aacaaatagc aatgtaataa acagtgcatt caaaaatatt tcgtttagat ctgttaaaat
    24661 taataacgta aatactcatt ctttaaaatt tattaactgc aattttttca atgttgatat
    24721 gataagagtt aatctcagca aatgtctttt tcatgaatgt agtatgcatg gtgtcaagat
    24781 aaaaccctgg ttgcctgtaa aatggacaaa agaattaata agtgattatt tgtatggctg
    24841 tttactatct ctgtactcta tctgtgcaag ggatatatat aatatgaacg ccgggaataa
    24901 cgtgaaggtt gctgcggatg ctttccttga aattattttc tctcttaaaa ataaatattg
    24961 tattaaacta ttaagcgctc aggatcgggc gtttatatac gagtttgcaa gaatgatctt
    25021 cgcttatatt aatgacaaaa gcattgagat cttattactt tcttgttttg cggcggctga
    25081 ccaaaaagca atccagcggt atagacctca gtcacaagat ggggaagact ttaggagtca
    25141 tttacaatat aagttgcctc tttccgctca ttaatgttgt tggtgcaaca taataagaca
    25201 ggtaagcggt accctataat gaaaccaata tcgggttatg caacggataa tcgtaaagac
    25261 ttaagtgggt gtttttgaca caatgagatg atttcctaaa ctgatcaaaa ccttcttccc
    25321 aacatggcat atcagtccga taatgcttta gagatcgaag gcttaacaaa caaggagatc
    25381 gatcatgcaa ttttctacaa ccccaactct ggaaggcctg accatcgttg aatattgcgg
    25441 tgtggtgacc ggcgaagcga ttttaggtgc caatattttc cgtgatttct ttgccggtat
    25501 ccgcgatatc gttggcggac gttccggtgc gtatgaaaaa gaactgcgta aagcacggga
    25561 gatcgccttt gaggaattag gctcccaggc gcgggcgctg ggggccgatg ccgtagttgg
    25621 tattgatatc gactacgaaa cggtcgggca aaacggcagt atgctgatgg ttagcgtcag
    25681 cggtacggcg gtgaaaacgc gtcgatgaga agagtcttct ggctggtcgc tgccgctctg
    25741 ttactggcag ggtgtgcagg cgaaaaaggc attgtcgaga aagagggata tcagcttgat
    25801 acccgacacc aggcgcaggc ggcgtatccg cgcattaaag tgctggtgat ccactacacc
    25861 gcagatgatt ttgatagctc gctggcgaca ctgactgata agcaggtcag ctcgcattat
    25921 ctgatccctg cggtgccacc gcgatacaac ggtaaaccgc gcatctggca actggtgccg
    25981 gaacaagaac tggcctggca tgcggggatt agcgcctggc gcggggcaac gcgccttaac
    26041 gacacctcta ttggcattga gctggaaaac cgtggctggc aaaaatcatc cggagtgaaa
    26101 tattttgccc cgtttgaaca ggcacagatt caggcgctta ttccgctggc gaaagatatt
    26161 attgcccgtt atcacatcaa gccggaaaac gtagtggcac atgcggatat cgcaccgcag
    26221 cgcaaagacg atccggggcc attatttccc tggcagcaac tggcgcagca ggggattggt
    26281 gcctggccgg atgcgcagcg ggtgaacttt taccttgccg ggcgcgcgcc gcacacaccg
    26341 gtagataccg cgtcattgct ggagcttttg gcgcgctacg gttatgacgt aaaacctgat
    26401 atgacaccgc gcgagcagcg gcgcgtgatt atggcattcc agatgcattt ccgcccgacg
    26461 ttatataacg gcgaagcgga tgcagaaact caggcgattg ccgaagcatt gctggagaaa
    26521 tacgggcagg attagcgcgg cagttttccg tggtcgcgta gccaggcggc agttttctcg
    26581 atgccttcat ccagggtgat gaccggctga taacctaact cttcctgcgc acgcgtaata
    26641 tccagcgtaa agtcaaaatt caacttggag gcgccgtagt gggtcagcgg cggctctttt
    26701 gctgacttgc ggcctaaacg ctccatgctg cgggcgatca tatccagcat cgggtaaggg
    26761 acggaacgaa tacgacagtc aatattcaac tcgtcgatca gcttctgcac gatgctgcgc
    26821 agtgtgcgat gctcgccgtt ggtgatgttg tacacacgcc cggaaggtag cttatcgcag
    26881 gcttcctggc ttgccagcca cattgcgtgc acggcatttt catagtaggt catatcgacc
    26941 agcgcactgc cgccatgcgg taacagaata ctgccgtagt ggtgcatcat atgcgccaga
    27001 cggggaataa agactttatc gtgcggtccg aacagacttt gtgggcgcag aatagtaaag
    27061 cgcgtttgtg gattcgcctg cgaaagcata ttgatcactt cttcgctggc tgctttgctg
    27121 cgggcaaact cgttggcgaa gcggtgaggg cgaaaatctt ctttaatatt gcgatggtgg
    27181 tgataatcga agtacaggga gggggaagag atatgaataa agttacgcac accccaggcg
    27241 acagcccatt cacccaggcg gcgagtggcg cgaacgttag ccagatcgaa agcctgttgt
    27301 gtcccccagg gtgaggtaaa gctggagcag tgccacagcg tatcaatgcc cgcgagcatc
    27361 actttagctt gtgatgaaac cagctcggtc agatccgtcg gaacaaactc tgcgcccatt
    27421 ttttccagca atttacccat tgcttcgttg cgaccgctcg cacgcacgct gatgcctttc
    27481 tggcataaaa actctaccgc gtttcgacct aagccgctgg tggcgccggt aaccagtacc
    27541 ttcatatcaa tccactgttg ttgagaaaat gacgtgcgca ttcttccgtg atttccccca
    27601 tgatgcaatg ggaaacatga aagaataacg caggttttgt cgattaatct gtgctttgtt
    27661 ctgccagtct ggcgatttgt tttgccattc cgcgaaaaat aaacagatgc gcggggatca
    27721 tcaataacca gtaaaacagc cccggcatac cgtgcggatg ccagaaagcg cggacatcga
    27781 tagtacgata gtcaccttta tcttccaggg taaaacacag tcgtcccagc cccggcgctt
    27841 tcatgccaaa taacaacgta agttgctttt tcggttcaac gacaatcact ttccagctat
    27901 ccaccgcatc gccggtctgt aaatattcgc gctccgggcg gcctttcgcc agattatgac
    27961 cgatcgcgcg gtccatcaac gcccgtgtct gccacaaaat attgccaaag aaataacgct
    28021 ctttaccgcc gatttggttc accaccagcc ataaagccgc aaggctggcg gacgttttaa
    28081 cggtaaaccc cgcctgtttg gcgaaataac cgtactccgg tcgccagcgg gcaaaggcct
    28141 gagcgtcgta gccccagtcg ctggagttga ccagtttttc ttcctctttc aacgtgctac
    28201 gtaccgcgtc atcgaaagcg atcagccgtt gtgggatgag tgcacgtagc gcggtatcat
    28261 ctgccagcag atcgtgtttc agcccctgaa tcaacgccct ggcggtggtg ggcggtacgg
    28321 aagtaatcac attgagaaac cacaccgaaa tccagcgggt ggggaggggg atggggatca
    28381 accagcggcg cttaccgctc accgccataa aatgttcaaa ctgttgctga taactgagca
    28441 cctctggtcc ggcggcttcg aagatgcggt gttcgttggc tggatggtct aacagcgcca
    28501 ccagatagtg cagcaagttt tccagcgcga tgggcgtggt gcgtgaacgt acccagcgtg
    28561 gcggcgttaa cactggcagg ttgtagacca tatcgcgcat gacttcgaac gccgctgaac
    28621 ctgcgccaac gataattccg gcccgaagtt cggtcactgg tacattcgct tcacgaagaa
    28681 tgtccgccgt agcctgacga gctcgcagat gatccgactg ctcatgtggc ggggcctgca
    28741 acgaactgag aaagattaat tgcttaactg gtacttcacg tagcgcatcg cggacgttga
    28801 gagccagctg gcgctcctga gcgataaaat cgccgccttc gcccatgctg tgcaccagaa
    28861 aatagacggt atcgatatcc tgcaacaggg ccggaaggtt atccggccag ctgagatcga
    28921 ctttatggca actgacgttt gccagttgca gctttgcaag cctgtcgaca tgacgtgccg
    28981 ccgctaggat ctgatgccct tgctggctga gtgtgcgcac cagatgctga ccaatgtagc
    29041 cactggcacc gagaactaaa atgcgttgcg gcacgtctct ctccttaacg cgccaggaat
    29101 gcgcgccagt gggcggcgac ttccgccagt tgttcgcgcg agacgtcaag atgcgtcacc
    29161 aggcggacaa tcggcgaggc gttaatcagc acgtttctcg ctttcatgta ttcgcctaac
    29221 gcggcagcat tttcttcccc gacgcgaaca aacagcatat tagtgtcctg acgcatcaca
    29281 tccgcgcctg cttcacgcag ttgctccgcc atccaggcgg cgttgtcgtg atcttcctgc
    29341 aaccgtgcga cgttattttc cagggcatac atcccggcgg cagccagaat cccggactga
    29401 cgcatcccgc caccggccat tttccgccag cggatggcac gtttaatgta atcacgatta
    29461 ccgacgagta atgaaccgac tggcgtccca agacctttcg acaggcaaat ggtgaacgaa
    29521 tcacaatatt gcgtgatctc tttcagttcg cagccgtaag ccaccacggc attaaagatg
    29581 cgcgcaccgt caacatgcag cgccagattg cgctcgcggg taaattccca tgcttctttc
    29641 aggtattcac gcggcagcac tttgccgttg tgggtgtttt ccagactgag taatttggtg
    29701 cgggcgaaat ggatatcgtc gggtttgatt ttcatcgcca ctttatccag cggtagcgtg
    29761 ccgtcggcag ccgcgtcgat gggttgcggc tgaatactgc ccagcaccgc tgcgccaccg
    29821 gcttcaaaca gatagttatg cgcggcctga ccgacaatat actcttcgcc acgttcgcag
    29881 tgactgagca gagcgaccag gttggcctga gtgccggtag gcagaaaaat ggcggcttct
    29941 ttaccggaaa gctctgctgc atagtcctgc agagcattaa cggtagggtc gtctccgtaa
    30001 acgtcgtccc caaccggggc ggccatcatc gcttcgagca tggcgcggct cggtcgggta
    30061 acggtatcac tgcgtaaatc aatcatggca tgtccttatt atgacgggaa atgccaccct
    30121 ttttacctta gccagttcgt tttcgccagt tcgatcactt catcgccgcg tccgctgata
    30181 attgcgcgca acatatacag gctaaaacct ttggcctgtt cgagtttgat ctgcggtgga
    30241 atggctaatt cttctttggc gaccaccaca tccaccaaca ccggaccgtc gatagagaag
    30301 gcgcgttgca gggcttcatc aacttcagag gctttttcta cacggatacc cgtaatgccg
    30361 cacgcttcgg caatgcgggc aaagttggtg tcgtgcagct cagtaccgtc tgtcaggtaa
    30421 cctccggcct tcatctccat cgccacaaag cccagcacgc tgttattaaa gacgataatt
    30481 ttcaccggca gtttcatctg cactactgag aggaaatcgc ccatcaacat gctaaaaccg
    30541 ccatcgccgc acatggcgac cacctgacgt tccggctcgg ttgcctgcgc acccaacgcc
    30601 tgcggcatgg cgttagccat cgaaccgtgg ttaaacgaac ctaacaggcg acgcttgccg
    30661 ttcattttca gataacgtgc cgcccatacc gttggcgtgc cgacgtcaca ggtaaagatg
    30721 gcgtcatcgg cggcaaaatg actaatttgc tgcgccagat attgcgggtg aatggctttc
    30781 tcgctcggtt tagctaaatc gtccagccct ttgcgggcat cgcggtaatc ttccagcgct
    30841 ttatccagaa acttgcgatc ggttttttct tccaccagtg gcagcaatgc gcgcagggtt
    30901 gatttgatat cgccgaccag cgccatatct accttgctat gcgcgccgat gctggctgga
    30961 ttaatatcaa tctgaataat tttggcatcg gtcgggtaga acgcacggta ggggaactga
    31021 gtaccgagta gcactaacgt gtcggcgttc atcatggtat ggaaacctga cgagaatccg
    31081 attaacccgg tcattccaac atcatacgga ttatcgtatt cgacatgctc tttaccgcgt
    31141 agggcatgaa caataggtgc tttaattttc ccggcaaact caactaactc tttatgcgcc
    31201 cccgcgcagc cgctgccaca catcagggcg atattgctgg aataacgcag cagttgcgcc
    31261 agtttgcgta actcttcttc ttccggcatc acgactggct gtggcgcatg ataccagtgc
    31321 gtggttgccc cttctggcgc aggttttaac gccacgtcgc ccggtaatac aacaacggaa
    31381 acgccacggt taagcaccgc tttgcgcata gcaattgcca gcacttgtgg gatctgctcc
    31441 gggctggaaa ccagctcgca atagtgacta cattcgcgga atagctcttg tgggtgggtt
    31501 tcctggaaat agccgctgcc aatttcgctg gagggaatat gagcggcaat cgccagtacc
    31561 ggaacgttat tgcggtggca atcgaatagg ccgttgatca agtgcaggtt gcctgggccg
    31621 cacgatccgg cacagaccgc cagttctcct gaaagttgtg cttcagcgcc agcggcaaac
    31681 gccgccactt cttcatggcg ggtggacatc cactcgatgg tgcccatgcg attaagactg
    31741 tcgctaagac cgttcagaga gtcgcccgtg actccccaga tgcgtttcac gcctgccgat
    31801 tcgagtgttt tggcgatata agctgcaacc gtttgtttca tggttctcca tctcctgaat
    31861 gtgataacgg taacaagttt agttcatctg acggaggggg aagggatggg agagaaagga
    31921 ggcactaacg gttaaatagc ccgatgaaag gaatatcatc gggcataagg cgattatgcg
    31981 agaaccaaat ccccctgcgg atggcaggag caggccagta cgtaaccttc agcgatttcg
    32041 gcgtcggtca gcgtcattgt actgctcacc gtatattcac cggaaaccac ttttgtctta
    32101 cagcagccgc aaacacccgc acggcaggca gcgacaaccg gaacgttatt gctttccagc
    32161 gcctccagta gcgtggtgcc aaccggggcg taaaattctc gtgccggttg cagtttggtg
    32221 aatttcagac cgctggtcgc cgcttctgct actggggtga agaatttctc tttaaagaaa
    32281 cgcgtcacgc cgagcgcttt cacttcctgc tctacccaat ccatatacgg agccgggccg
    32341 caggtcatca cggtacgtga agctaagtca ggtacatctg ccagcagttc gcgagtgagg
    32401 cgaccagcga taaagccttc ggtaacgtta ttttctgcca ccagcgttac cggatagtta
    32461 cgccactcat cggcgaaaat aacatcctgc ggcgtacgca cgttgtagat cacccgcaca
    32521 tcggcctgtg gacggttctt cgcaagccag cgacgcatcg acataatcgg cgtgacgccg
    32581 cagcctgccg ccagcaacag gaatttatct tctgctttat cgtcgcaggt aaattccccc
    32641 atcgcgtccg aaagccagag ataatcaccg cgttttacat cgcgcgtcag ccactgggag
    32701 ccgacaccgt catcaatccg ccgcacggtc agggtgatat attcactcac gcctggcgtg
    32761 gaggaaatgg tgtaagcacg cagcgtttcc gctgagtttc gcacgctgac cagtgcatat
    32821 tgcccggcgc gatatgggta gtgatcgtgg caaatcaggg aaatcgtcca cacatccggc
    32881 gtttcttgcg taatgtgatg aacctgtatc cgccacgggc attgattcgt tggcatcgtc
    32941 atcgacaaac tccttacgcg ttcaacagtt gcttcatgtc ttcttcaaca gtggtgatag
    33001 aacgcaggcc gaatttctcg ttcagcaccg ccagcaggtc tggtgtcagg aaaccaggtg
    33061 cagtcgggcc ggtgacgata tttttcacgc ccagagaaag cagcgtcagc agaatgacaa
    33121 tcgctttctg ttcaaaccag gagagcacca gcgacagcgg cagatcgttc acaccacagc
    33181 ccagtttctc tgccagagtg acagccagaa taattgctga gtaagcatcg ttacattgac
    33241 ctgcatctac cagacgcggc agaccttcga tatcgccaaa ctcaagtttg ttaaagcgat
    33301 atttaccaca ggcgagggtc aggatcaggc agtcatccgg cacgctggtg gcgaaatcgg
    33361 tgaagtagtg gcgctcgccg cgtgcgccgt cacagccacc aagcaggaag atatgacgca
    33421 gtttttcacg gctaaccaga tcaatcagcg tatcggctgc accaagcagc gtctggcgac
    33481 caaagcccac ggtgatcagg tgaggaattt cgctgtacgg gaagcccgcc atttgttgcg
    33541 cctgggcgat gaccgcagag aaatcttcgc catccagatg acgcacgcca ggccagccaa
    33601 cgatgctgcg ggtccagata cgatcgtcat aagcgcctac ggttgggtcg atgatgcagt
    33661 tcgaggtcat cacgatgggg ccagggaaac gagcgaactc cacttgctga ttctgccagc
    33721 cgctgccgta gttaccgacc agatgcttga atttacgcag ctccgggtag ccatgcgcag
    33781 gcagcatttc gccgtgggtg tagacattaa cgcccgtgcc ttcggtctgt tccagcaggt
    33841 tgtagagatc tttgagatcg tgaccggaaa tcagaatgca tttacccgcc gtcgctttga
    33901 cgttgacctg ggttggcgtc gggtgaccgt atttaccggt ttcgcctgca tccagaatgc
    33961 tcatcacttt gaagttcatc tggccgattt ccattgaaca ctcaagaagc gcgttcatat
    34021 cggcaggcca ggtccccagc cacgccatga ttttatggta ctgggcataa atatcgttgt
    34081 cgtattgacc gagaacatgc gcgtgttcca tataggccgc cgcacctttc aggccataca
    34141 ggcacagcag acgcaggccg agaatgtttt cgccaatcgc cgctttatct ttgttagggg
    34201 taaattctgc tgcctgacgt tgcagctcgc cgagatcatc gctcaccagt tgcaggtcag
    34261 ccatcgggtt atcgacgcgc gcgttggcat ctacagccag gcattgtgct ttcagcgcct
    34321 cgcgcaaggc aatcgcttca cgagcgtagc cgacaatacg cggagaatcg aagttaacgt
    34381 tggtcagggt tgagaaaaag gcacgtggcg cgaagctgtc aacatcgtgg ttgatgatgc
    34441 cgtattcacg cgcttttacc gcccaggcag aaagcccttg cagcgccgcg atgagtaaat
    34501 cctgaaggtc agaagtttcc gccgttttac cacacatccc ctgcgcgtat gagcagccgt
    34561 tttctgccgg agtacggata gtttgttcac attgcacaca aaacatgatc acacctttta
    34621 aagttatatt taatatacat gtttaagggt aagacgctta acgcggggat aaaagggatt
    34681 tttcatgcaa ctttaaggga gattgattta gcgcaatttt ggcggcaggg aactaccgcc
    34741 agagaggtat tacgcagaga aaaaggcgat gaggatcggc actaacaggc taagaataaa
    34801 accgtgaaca attgccgccg ggaccatatc cagcccgcca gtacgttgaa gaacgggcag
    34861 ggtgaaatcc attgatgtgg caccgcataa gcccagtgca gtagagcggc tgcggcgaat
    34921 cagcccaggg atcaacataa tagcaatcag ttcacgggcc agatcattaa aaaacgccgc
    34981 gctcccgatt accggaccaa aagattcggt caataaaata ccggaaagag aataccagcc
    35041 gaaaccggag gccattgcca gcgcggtatt gatggggaga tcaagaataa aggcgttaat
    35101 taaaccacca attaatgaac tgacaaccac caccacggcg acaatcattc cccggcgatt
    35161 aaggacaatc tgctttaagg tcatgccatt attgcgcaac tgaataccaa cgaggaaaag
    35221 tagcaaaatt aacgtgtatt cactggcttc ggtcgcgtgt tgtaagaaag ccagtccact
    35281 cagaccaatg gcaaaaccaa tcactactac gccgcacagt tttagcgact ccagcgccat
    35341 cgcaatacgc gacgggagtt tttcttgctg atggtggttg cgccacggca ggcctcgctc
    35401 cagccacatc agggcggcaa tattacacag taaaataacg gtaatactga cggcagaata
    35461 atgcagaatc gccaacaggt tactggcgag gttatcgaga aacgccagac tgatacccat
    35521 aaaaaagaga ataaggtaaa ccatccagct taatagctga ttaatagctt ttaacgcagc
    35581 ttgttggcga agcggaatga ggtaacccac aatcagggga accagaatga ttaacagccc
    35641 agaaaacatg aaaatccagt ccttgcaaag aagaagtcga aatgcgcgat gacacactac
    35701 tgaaagcgga agggcgagta aagttgcaat taaaaggaaa tgttatgcat cagagggg
//
LOCUS       DAJTSA010000031        35006 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000031 DAJTSA010000000
VERSION     DAJTSA010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35006
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.031"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..825)
                     /locus_tag="OO369_001695"
     CDS             complement(<1..825)
                     /locus_tag="OO369_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5982009.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSSPDY"
     gene            917..1003
                     /locus_tag="OO369_001696"
                     /pseudo
     CDS             917..1003
                     /locus_tag="OO369_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1408..1884)
                     /locus_tag="OO369_001697"
     CDS             complement(1408..1884)
                     /locus_tag="OO369_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR5982010.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1943..3232)
                     /gene="bioA"
                     /locus_tag="OO369_001698"
     CDS             complement(1943..3232)
                     /gene="bioA"
                     /locus_tag="OO369_001698"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR5982011.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3319..4359
                     /gene="bioB"
                     /locus_tag="OO369_001699"
     CDS             3319..4359
                     /gene="bioB"
                     /locus_tag="OO369_001699"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR5982012.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4356..5510
                     /gene="bioF"
                     /locus_tag="OO369_001700"
     CDS             4356..5510
                     /gene="bioF"
                     /locus_tag="OO369_001700"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR5982013.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5497..6252
                     /gene="bioC"
                     /locus_tag="OO369_001701"
     CDS             5497..6252
                     /gene="bioC"
                     /locus_tag="OO369_001701"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR5982014.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6245..6922
                     /gene="bioD"
                     /locus_tag="OO369_001702"
     CDS             6245..6922
                     /gene="bioD"
                     /locus_tag="OO369_001702"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5982015.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7501..9522
                     /gene="uvrB"
                     /locus_tag="OO369_001703"
     CDS             7501..9522
                     /gene="uvrB"
                     /locus_tag="OO369_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR5982016.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9714..10622)
                     /gene="yvcK"
                     /locus_tag="OO369_001704"
     CDS             complement(9714..10622)
                     /gene="yvcK"
                     /locus_tag="OO369_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR5982017.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10887..11031
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11019..12008
                     /gene="moaA"
                     /locus_tag="OO369_001705"
     CDS             11019..12008
                     /gene="moaA"
                     /locus_tag="OO369_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR5982018.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12030..12542
                     /gene="moaB"
                     /locus_tag="OO369_001706"
     CDS             12030..12542
                     /gene="moaB"
                     /locus_tag="OO369_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR5982019.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12545..13030
                     /gene="moaC"
                     /locus_tag="OO369_001707"
     CDS             12545..13030
                     /gene="moaC"
                     /locus_tag="OO369_001707"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR5982020.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13023..13268
                     /gene="moaD"
                     /locus_tag="OO369_001708"
     CDS             13023..13268
                     /gene="moaD"
                     /locus_tag="OO369_001708"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR5982021.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13270..13722
                     /gene="moaE"
                     /locus_tag="OO369_001709"
     CDS             13270..13722
                     /gene="moaE"
                     /locus_tag="OO369_001709"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR5982022.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13859..14563
                     /locus_tag="OO369_001710"
     CDS             13859..14563
                     /locus_tag="OO369_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5982023.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14768..15481
                     /locus_tag="OO369_001711"
     CDS             14768..15481
                     /locus_tag="OO369_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5982024.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15517..16473)
                     /locus_tag="OO369_001712"
     CDS             complement(15517..16473)
                     /locus_tag="OO369_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR5982025.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16473..17714)
                     /gene="clsB"
                     /locus_tag="OO369_001713"
     CDS             complement(16473..17714)
                     /gene="clsB"
                     /locus_tag="OO369_001713"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR5982026.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17711..18472)
                     /locus_tag="OO369_001714"
     CDS             complement(17711..18472)
                     /locus_tag="OO369_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5982027.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18605..19015
                     /locus_tag="OO369_001715"
     CDS             18605..19015
                     /locus_tag="OO369_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR5982028.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18977..20083)
                     /locus_tag="OO369_001716"
     CDS             complement(18977..20083)
                     /locus_tag="OO369_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5982029.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20094..21227)
                     /locus_tag="OO369_001717"
     CDS             complement(20094..21227)
                     /locus_tag="OO369_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5982030.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21220..22956)
                     /locus_tag="OO369_001718"
     CDS             complement(21220..22956)
                     /locus_tag="OO369_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5982031.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLEPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22949..23944)
                     /gene="hlyD"
                     /locus_tag="OO369_001719"
     CDS             complement(22949..23944)
                     /gene="hlyD"
                     /locus_tag="OO369_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR5982032.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23947..24618)
                     /gene="cecR"
                     /locus_tag="OO369_001720"
     CDS             complement(23947..24618)
                     /gene="cecR"
                     /locus_tag="OO369_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR5982033.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24847..26034
                     /gene="rhlE"
                     /locus_tag="OO369_001721"
                     /pseudo
     CDS             24847..26034
                     /gene="rhlE"
                     /locus_tag="OO369_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26241..26723)
                     /gene="ybiA"
                     /locus_tag="OO369_001722"
     CDS             complement(26241..26723)
                     /gene="ybiA"
                     /locus_tag="OO369_001722"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR5982034.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26843..28993
                     /gene="dinG"
                     /locus_tag="OO369_001723"
     CDS             26843..28993
                     /gene="dinG"
                     /locus_tag="OO369_001723"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR5982035.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29021..29983
                     /gene="ybiB"
                     /locus_tag="OO369_001724"
     CDS             29021..29983
                     /gene="ybiB"
                     /locus_tag="OO369_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR5982036.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30124..31209
                     /gene="hcxB"
                     /locus_tag="OO369_001725"
     CDS             30124..31209
                     /gene="hcxB"
                     /locus_tag="OO369_001725"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR5982037.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31438..31698)
                     /locus_tag="OO369_001726"
     CDS             complement(31438..31698)
                     /locus_tag="OO369_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5982038.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31963..32229)
                     /gene="ybiI"
                     /locus_tag="OO369_001727"
     CDS             complement(31963..32229)
                     /gene="ybiI"
                     /locus_tag="OO369_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR5982039.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32303..32980)
                     /gene="ybiX"
                     /locus_tag="OO369_001728"
     CDS             complement(32303..32980)
                     /gene="ybiX"
                     /locus_tag="OO369_001728"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR5982040.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LQEWSEI"
     gene            complement(33022..35006)
                     /locus_tag="OO369_001729"
                     /pseudo
     CDS             complement(33022..35006)
                     /locus_tag="OO369_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8294 a         8973 c         9458 g         8281 t
ORIGIN      
        1 gtagtccggc gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt
       61 gttataaata ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt
      121 cagctgattg ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg
      181 tagttggttt tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg
      241 taatctgtta tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt
      301 aagctggttg ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc
      361 caggctattg agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt
      421 taaaactctg ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct
      481 gctcagtctg ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga
      541 gcgtagctca ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga
      601 gcgtaatctg agtaaactta aattgagggc tggctcttga aaagccagac atattttcag
      661 taatcgaact gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca
      721 gatgcggtga taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa
      781 accacaaaag gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc
      841 attgtttata tattaatata ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc
      901 tgtaaatagt taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt
      961 gaagttctgg ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt
     1021 catcaagcca gtagtctgcc caccacagca tcatttctct gcgcttatcg agatactgag
     1081 catggttgta aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa
     1141 aatcaccttg cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat
     1201 agtgactgga tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat
     1261 tataatgcat tgatattaat gatttttact gtttctcggt cagctttttt atactaactt
     1321 gagcgaaacg ggaaggtaaa aagacaaaaa gttgttttta atacctttaa gtgataccag
     1381 atggcattgc gccatctggc agagtgatta actaaacatc gcggtaatcg atgcgcttgc
     1441 cagagagtgg aaatgaacgt taaacccgac catcgcgccg ctggctcctt catcgacatc
     1501 aatacgctct acatccagcg cgtgaacggt aaaaatgtag cgatgggttt cgcctttcgg
     1561 cggcgctgcg ccatcgtatc cggttttacc aaaatcggta cgcgtctgca aaacgccgtc
     1621 tggcattgct accagaccag agccaaaccc ttgcggtaat acgcgggtat cagcgggtaa
     1681 attaacaact acccagtgcc accagccgga gccggttggc gcatccgggt cgtagcaggt
     1741 gacaacaaaa cttttcgttc ccgcaggaac atcatcccac gccagatgcg gtgaaatatt
     1801 atcgccatcg taacccatgc cgttaaagac atgacgatgc ggcaacttat cgccatcgcg
     1861 cagatcgtta ctgatgagtt tcatgaaccc tcctttcttg tttgcagaaa gtgtagccag
     1921 aaaccctcac gctgacttcc cgttattggc aaaaaaatgt ttcatcctgt accgcgcggt
     1981 taaccgctgc ggtcagacgc tgcaactgtt gcgggagaat aatatagggc ggcatcaggt
     2041 aaatcagttt gccaaaaggc cggatccata ccccctgttc gacaaagaat ttttgcagcg
     2101 ccgccatatt caccggacga gtggtttcga ccacaccgat tgcccccagt acgcgcacat
     2161 cggcaaccat ttcggcatca cgggctggtg ccagttgctc gcgcagctgc acttcaatag
     2221 ccgccacctg ctgctgccat tcgccggatt cgataatcgc taggctggcg tttgctgccg
     2281 cgcaggccag cggattgccc ataaaagttg gcccatgcat aaagcagccg gcttcgccgt
     2341 tactgatggt ttctgcaacc tcgcgcgtgg taagtgtggc ggaaagggtc attgtgccgc
     2401 cggttaaggc tttaccgagg cacaaaatgt ccggcgcgat ttctgcatat tcacaggcaa
     2461 acagtttgcc ggtacgacca aatccggtgg cgatctcgtc ggcaatcagc aagatacctt
     2521 cgcgatcgca cattttgcgg attcgtttta accattccgg atggtacatg cgcatcccgc
     2581 ctgcgccctg gacaatcggc tcaatgatca ccgctgcgat ttcatgacga tgcgccgcca
     2641 tcaggcgggc aaaaccaacc atatcgcgtt catcccattc accatccatg cggctttgcg
     2701 gggcgggagc aaacaagttt tctggcagat agcctttcca caggctgtgc attgagttgt
     2761 ccggatcgca caccgacatc gcgccaaagg tatcgccatg ataaccattg cggaaggtca
     2821 gaaaacgctg gcgcgcttcg cctttggctt gccagtactg caacgccatt ttcatcgcca
     2881 cttccaccgc tacggaaccg gagtccgcga gaaaaacgca ctccagcggt tgcggcgtca
     2941 ttgccaccag tttgcggcat agctcgatgg cgggcgcatg ggtgataccg ccaaacatca
     3001 catgcgacat ggcatcaatt tgcgtcttca tcgccgcatt aagctgcggg tgattgtagc
     3061 cgtggatcgc cgcccaccag gacgacatac cgtcaaccag gcgtctgccg tcagacaaaa
     3121 ttagctcgca accttcggcg ctcgccaccg gataaaccgg cagaggggag gtcatggatg
     3181 tgtatgggtg ccagatatgg cgttggtcaa aggcaagatc gtccgttgtc ataatcgact
     3241 tgtaaaccaa attgaaaaga tttaggttta caagcctaca ccgaattaac aacaaaaaac
     3301 acgttttgga gaagccccat ggctcaccgc ccacgctgga cattgtcgca agtcacagaa
     3361 ttatttgaaa aaccgttgct ggatctgctg tttgaagcgc agcaggtgca tcgccagcat
     3421 ttcgaacctc gtcaggtgca ggtcagcacg ttgctgtcga ttaagaccgg agcttgtccg
     3481 gaagattgca aatactgccc gcaaagctcg cgctacaaaa ccgggctgga agccgagcgg
     3541 ttgatggaag ttgaacaggt gctggagtcg gcgcgcaaag cgaaagcggc aggatcgacg
     3601 cgcttctgta tgggcgcggc atggaagaat ccccacgaac gcgatatgcc gtacctggaa
     3661 caaatggtgc agggggtaaa agcgatgggg ctggaggcgt gtatgacgct gggcacgttg
     3721 agtgaatctc aggcgcagcg cctcgcgaac gccgggctgg attactacaa ccacaacctg
     3781 gacacctcgc cggagtttta cggcaatatc atcaccacac gcacttatca ggaacgcctc
     3841 gatacgctgg aaaaagtgcg cgatgccggg atcaaagtct gctcaggtgg cattgtgggc
     3901 ttaggcgaaa cggtaaaaga tcgcgccgga ttattgctgc aactggcaaa cctgccgacg
     3961 ccgccggaaa gcgtaccaat caacatgctg gtgaaggtga aaggcacgcc gcttgccgat
     4021 aacgatgacg ttgatgcctt tgattttatt cgcaccattg ccgtagcgcg gatcatgatg
     4081 ccgacctctt acgtgcgcct ttctgccgga cgcgagcaga tgaacgaaca gactcaggcg
     4141 atgtgcttta tggcaggcgc aaactcgatt ttctacggtt gcaaactgct gaccacgccg
     4201 aatccggaag aagataaaga cctgcaactg ttccgcaaac tggggctaaa tccgcagcaa
     4261 actgccgtgc tggcagggga taacgaacaa cagcaacgtc ttggacaggc gctgatgacc
     4321 ccggacaccg acgaatatta caacgcggca gcattatgag ctggcaggag aaaatcaacg
     4381 cggcgctcga tgcgcggcgt gctgccgatg ccctgcgtcg ccgttatccg gtggcgcaag
     4441 gagccggacg ctggctggtg gcggatgatc gccagtatct gaacttttcc agtaacgatt
     4501 atttaggttt aagccatcat ccgcaaatta tccgtgcctg gaagcagggg gcggagcaat
     4561 ttggcatcgg tagcggcggc tccggtcacg tcagcggtta tagcgtggtg catcaggcgc
     4621 tggaagaaga actggccgag tggcttggct attcgcgggc gctgctgttt atctctggtt
     4681 ttgccgcgaa tcaggcagtc attgccgcga tgatggcgaa agaggaccgt attgttgccg
     4741 accggcttag ccatgcctca ttgctggagg ctgccagttt aagcccgtcg cagcttcgcc
     4801 gttttactca taacgatgtc gctcatctgg cgcgactgct tgcttccccc tgtccggggc
     4861 agcaactggt ggtgacagaa ggcgtgttca gcatggacgg cgatagcgcg ccactggcgg
     4921 aaatccagca ggtaacgcaa cagcacaatg gctggttgat ggtcgatgat gcccacggca
     4981 cgggcgttat cggggagcag gggcgcggca gctgctggct gcaaaaggta aaaccagaat
     5041 tgctggtagt gacttttggc aaaggatttg gcgtcagtgg ggctgcggtg ctttgctcaa
     5101 gtacggtggc ggattatctg ctgcaattcg cccgccacct tatctacagc accagtatgc
     5161 cgcccgctca ggcgcaggca ttacgtgcgt cgctggcggt cattcgccgt gatgaggggg
     5221 atgcacggcg cgaaaaactg gtgtcactca ttgcgcgttt tcgtgccgga gtacaggatt
     5281 tgccgtttac gctggctgat tcatgcagcg ccatccagcc attgatcgtc ggtgataaca
     5341 gccgtgcgtt acaactggca gaaaaactgc gccagcaagg ctgctgggtc acggcgattc
     5401 gcccgccaac cgtacccgct ggtactgcgc gactgcgctt aacgctaacc gctgcgcatg
     5461 aaatgcagga tatcgaccgt ctgctggagg tgctgcatgg caacggttaa taaacaagcc
     5521 attgcagcgg catttggtcg ggcagccgca cactatgagc aacatgcaga tctacagcgc
     5581 cagagtgctg acgccttact ggcaatgctt ccacagcgta aatacaccca cgtactggac
     5641 gcgggttgtg gacctggctg gatgagccgc cactggcggg aacgtcacgc gcaggtgacg
     5701 gcgttagatc tctcgccgcc aatgcttgcg caagcccgcc ataaagatgc cgcagatcat
     5761 tatctggcag gagatatcga atccctgccg ttagcgactg cgacgttcga tcttgcatgg
     5821 agcaatctcg cggtgcagtg gtgcggtaat ttatccacgg cactccgcga gctgtatcgg
     5881 gtggtgcgcc ccggaggcgt ggtcgcgttt accacgctgg tgcagggatc gttacccgaa
     5941 ctgcatcagg cgcggcaggc ggtggacgag cgtccgcatg ctaatcgctt tttaccgcca
     6001 gatgaaatcg aagagtcgct gaacggcgtg cgttatcaac atcatattca gctcatcaca
     6061 ctatggtttg atgatgcgct cagtgccatg cgttcgctga aaggcatcgg tgctacgcat
     6121 cttcatgaag ggcgcgaccc gcgaatatta acgcgttcgc agttgcagcg attgcaactg
     6181 gcctggccgc aacaacaggg gcgatatcct ctgacgtatc atcttttttt gggagtgatt
     6241 gctcgtgagt aaacgttatt ttgtcaccgg aacggatacc gaagtgggga aaaccgtcgc
     6301 cagttgtgca cttttacaag ccgcgaaggc agtaggttat cggacggcag gttataaacc
     6361 ggtcgcctct ggcagcgaaa agaccccgga aggcttacgt aatagtgacg cgctggcgtt
     6421 acagcacaac agcagcctgc agctggatta cgcaacggta aatccttaca ccttcgcaga
     6481 atccacctcg ccgcacatca tcagcgccca agagggcagg ccgatagaat cattggtaat
     6541 gagcgccgga ttacgcgcgc ttgaacaaca ggctgactgg gtgttagtgg aaggtgctgg
     6601 cggctggttt acgccgcttt ctgacacttt cacttttgca gattgggtaa cacaggaaca
     6661 actgccggtg atactggtag ttggtgtgaa actcggctgt attaatcacg cgatgttgac
     6721 tgcgcaggca atacaacacg ccggactgac tctggcgggt tgggtggcga acgatgttac
     6781 gcctccggga aaacgtcacg ctgaatatat gaccacgctc acccgcatga ttcccgcgcc
     6841 gctgctggga gagatcccct ggcttgcaga aaatccagaa aatgcggcaa ccggaaagta
     6901 cataaacctt gccttgttgt agccattctg tatttggtta aattgcgagc gagatcgcgt
     6961 cttcgattga ctgcaattta accaattaaa ttctaaaata atcacgaaaa aaattttact
     7021 tccgcctcat gcggcgaatg tgggaattgc ccaggcggcg ggggataggg gctggagaca
     7081 gttatccact attcctgtgg ataaccatgt gtattagagt tagaaaacac gaggcaagcg
     7141 agagaatacg cggcttgcac gcgaattggc gttaaagacg gctcaaagaa atatctttta
     7201 ttttttaact ggttagataa atgcaatggc agtcactgaa caggcatctc ttgccataaa
     7261 actgtcatca ctcatcttga caaatgttaa aaaagccgtt gctttgggga taacccggta
     7321 aggctggagt tttatctcgc cacagagtaa attttgctca tgattgacag cggagtttac
     7381 gctgtatcag aaatattatg gtgatgaact gtttttttat ccagtataat ttgttggcat
     7441 aattaagtac gacgagtaaa attacatacc tgcccgccca actccttcag gtagcgactc
     7501 atgagtaaac cgttcaaact gaattccgct tttaaacctt ctggcgatca gccagaggcg
     7561 attcgacgtc tcgaagaggg gctggaagat ggcctggcgc accagacgtt acttggcgtg
     7621 actggctccg ggaaaacctt caccattgcc aatgtcattg ctgaccttca gcgcccaacc
     7681 atggtacttg cgcccaacaa aacgctggcg gcccagctgt atggcgaaat gaaagagttc
     7741 ttcccggaaa acgcggtgga gtatttcgtc tcctactacg actactatca gccggaagcc
     7801 tatgtaccga gttccgacac cttcattgag aaagatgcct cggttaacga acatattgag
     7861 cagatgcgtt tgtccgccac caaagcgatg ctggagcggc gtgatgtgat tgtagtggcg
     7921 tctgtttccg cgatttatgg tctgggcgat cctgatttat atctcaagat gatgctccat
     7981 ctcacggtcg gtatgattat cgatcagcgc gcgattctgc gccgactggc ggagctgcaa
     8041 tacgctcgta atgatcaagc attccagcgt ggtactttcc gcgttcgtgg cgaggtgata
     8101 gatatcttcc cggcagaatc ggatgacatt gcacttcgcg tggaactgtt tgacgaggaa
     8161 gtggaacgat tgtcgttatt tgacccgctg accgggcaga ttgtttccac tattccacgt
     8221 tttaccatct acccgaaaac gcactacgtc acaccgcgcg agcgcatcgt acaggcgatg
     8281 gaggagatca aagaagaact ggccgccaga cgcaaagtgc tgttggaaaa caacaaactg
     8341 ctggaagagc agcggctgac ccagcgtacc cagtttgatc tggagatgat ggacgagctg
     8401 ggctactgtt cggggattga aaactactcg cgcttcctct ccggtcgtgg accgggtgag
     8461 ccaccgccga cgctgtttga ttacctgcct gccgatgggc tgctggtcgt cgatgaatct
     8521 cacgtcacca ttccacaaat tggcggcatg tatcgcggtg accgggcgcg taaagagaca
     8581 ctggtggagt acggcttccg cctgccatca gcgctggata accgtccgct taagtttgaa
     8641 gagttcgaag cattagcgcc gcaaaccatc tatgtttcgg cgacgccggg taattacgag
     8701 ctggaaaaat ccggcggcga tgtggtggat caggtggtgc gtccaaccgg attgcttgac
     8761 ccgattatcg aagtgcggcc ggtggcgaca caggttgatg atcttctttc ggagattcgt
     8821 cagcgagcgg caattaacga acgcgtgctg gtcaccacac tgaccaagcg aatggcggaa
     8881 gatcttaccg aatatctcga agaacacggt gagcgcgtgc gttatcttca ctcagatatc
     8941 gacaccgtcg agcgtatgga gattatccgc gacttgcgtc tgggtgagtt cgacgtattg
     9001 gtagggatca acttactgcg tgaaggtctg gatatgccgg aagtgtcgct ggtggcgatc
     9061 ctcgacgctg acaaagaagg cttcctgcgt tccgaacgtt cgttgatcca gaccattggt
     9121 cgtgcggcac gtaacgttaa cggtaaagcg attctctacg gcgataggag cactccatca
     9181 atggcgaaag cgattggcga aaccgaacgt cgccgtgaga aacagcagaa gtacaacgag
     9241 gaacacggca ttacgccgca aggcttgaac aagaaagtgg tcgatatcct ggcgctgggg
     9301 cagaacattg ccaaaaccaa agcgaagggc agaggaaaat cgcgcccgat tgttgagccg
     9361 gataatgtgc cgatggatat gttgccgaaa gcgttgcagc agaaaatcca tgaactggaa
     9421 gggctgatga tgcaacacgc gcagaatctg gagttcgaag aagcggcaca aattcgtgac
     9481 cagttgcatc agttgcgtga gttgtttatt gccgcatcgt aacaggatag cgaagaagac
     9541 tgatgacaaa cggaaaacag tctgatgcgc tacgcttatc aggcctacat tttctccgca
     9601 atatattgaa tttgcgcggt ttgtaggccg gataaggcgt tcacgccgca tccggcataa
     9661 acaacgcgca ctttgtcttc aataggaagc cggaattttc cttcggattt ccgttaacct
     9721 aaagcctgta acgccttttc cagcgcgtta tgtaacaact ggcggtcatg acgatacgga
     9781 atatcgctgg cctccagtac ctcctggatc acaatccgct ctttcaccgc cgagacatcc
     9841 acttttggcc cgacgatgac cgcatcaatg acctttttac caacatactg ctccataatt
     9901 gccagcttgc tttccagctt caaattagcc gcaggtaaac tcaactcacg ccccagattg
     9961 ccgatataaa ccatcggcgc tggcgtgcgg cgtaatgcct gggcgatttc cttcagcagc
    10021 agaattggca tcaggctggt ataaaaactg ccaggcccaa taatgatgag atccgcttca
    10081 ttgatagcgt gaaccgcctc acgcgttgcg ggtacattag gcgttaacaa taactcttga
    10141 atcggcgtag ttaactggtc gatattgacc tcgccgtaaa cttcatgccc ctgatcgtca
    10201 atcgccatca gatcaacagg atgctctgac attggaatca aatgcgtatc cactttcagc
    10261 agattacgaa ttaaattgat ggcttccaga ggccgcacgc taaggtgatc cagcgccttt
    10321 aacatcaagt ttccgagatt atgaccggaa agttcgccat tgccgccaaa acggtattca
    10381 aacatcgcgg aggcgacgtt cggttccgtt atcagctggt tgaggcagtt gcgcatatcg
    10441 ccccaagcaa tgccgccttc tgaacggcga atacgccccg tcgagccacc attatccgtg
    10501 gtggtgacga tacccgttaa acgagaaccc aaagacgaaa gtgatgagag aacgcgtccc
    10561 agtccatgcc ctccgccgag agcaacgaca cgatcaagat cagccagcgt acgattgcgc
    10621 atatagtttc ctggagtcag attatccgcg ctacagtagc gcaaattgcg gggaaacagc
    10681 aattaacctg ccaatattga ggatacaaac tattttcttc tatctctaaa ggacgatgca
    10741 cgctatgcct ccctgatgat gtatatcaaa gtaaaaccgc cattttccct tattctgtag
    10801 cgaaatagca cgatcatgac gctatataca tgattacata gcgaaagtgt ggatggtaaa
    10861 aatctcattt acacgctagt atcggcttaa ccactaaaca ctctagcctc tgcacctggg
    10921 tcaactgata cggtgctttg gccgtgacaa tgctcgtaaa gattgccacc agggcgaagg
    10981 aagaaatgac ttcgcctccc gtatctggaa aggtgtacat ggcttcacaa ctgactgatg
    11041 tatttgcgcg taagttttac tacttgcgcc tgtcgattac cgatgtgtgt aactttcgtt
    11101 gcacctactg cctgccggat ggctacaaac cgagcggcgt caccaataaa ggctttctta
    11161 ccgtcgatga aattcgccgg gttacgcgcg ccttcgccag tctgggcacc gaaaaagtgc
    11221 gcctgacagg aggagagccg tctttacgcc gcgactttac cgatatcatc gccgctgtgc
    11281 gggaaaacga cgctatccgc cagattgcgg tcaccaccaa tggttaccgt ctggaacgcg
    11341 atgtggcgaa ctggcgcgat gcgggactta ctggcattaa cgtcagtgtc gacagtttgg
    11401 acgcccgcca gtttcacgct attaccgggc aggataaatt caaccaggtc atggcaggga
    11461 ttgatgctgc atttgaggcc ggttttgaga aggtcaaagt caataccgtg ctgatgcgtg
    11521 atgttaatca tcaccagctc gacacctttc tgaactggat ccagcatcgc cctatccagc
    11581 tgcgtttcat cgaactgatg gaaacgggcg agggcagcga gctcttccgt aagcatcaca
    11641 tctctggtca ggttctgcgt gacgagctac tgcgtcgcgg ctggatccac caattacgtc
    11701 aacgcagcga cggtcccgcg caagtctttt gccatccaga ttacgccgga gagattggcc
    11761 ttatcatgcc gtatgaaaaa gacttctgcg ccacttgcaa ccgcctgcgc gtttcctcca
    11821 ttggtaaact ccatctctgc ctgtttggtg aaggcggcgt taacctgcgc gatctgctgg
    11881 aaggcgatac ccagcaacag gcgctggaag cgcgtatttc agcggcgctg cgggagaaga
    11941 aacagaccca tttcctgcat caaaacaaca ccggtattac gcaaaactta tcgtacattg
    12001 gcggctaaaa cgtcaaaagg agagatcaga tgagtcaggt aagcactgaa tttatcccga
    12061 cccgtattgc tattcttacg gtttctaatc gtcgcggtga agaagacgat acctccggtc
    12121 actatctgcg cgattcggcg caagaagcgg gccatcacgt tgtcgatgaa gccattgtga
    12181 aagaaaaccg ctacgctatt cgcgctcagg tatctgcgtg gatcgccagc gacgatgtac
    12241 aagtggtatt gattacgggt ggtactggcc tgacggaagg tgatcaggct cccgaagcgc
    12301 tgctgccgtt gttcgaccgt gaggttgaag gttttggtga agtgttccgt atgttgtcgt
    12361 ttgaagagat tggcacttcc acgttgcaat ctcgtgcggt agcgggcgtc gctaacaaaa
    12421 cgctgatttt cgccatgccg ggttcgacca aagcgtgccg taccgcatgg gaaaatatca
    12481 tcgcgccgca gctggatgcc cgtacgcgtc cgtgtaattt ccatccacat ttgaagaaat
    12541 aagtatgtcg caactgaccc atatcaacgc cgctggcgaa gcgcacatgg tggatgtctc
    12601 cgccaaagcg gaaaccgtgc gtgaagcgcg ggcggaagcc tttgtcacca tgcgcagcga
    12661 gacgctggcg atgattattg atggtcgcca ccacaaaggc gacgtatttg ccactgcgcg
    12721 tattgccggt attcaggcgg caaaacgcac ttgggatctg atcccgctgt gtcacccgct
    12781 gatgctcagc aaagttgaag tcaatttaca ggccgagccg gagcacaatc gggtgcgtat
    12841 agaaacctta tgccgcctga ccgggaaaac cggtgtcgaa atggaagcgt taaccgcggc
    12901 ctccgtggcg gcgctgacca tttatgacat gtgcaaagcg gtgcaaaaag atatggtgat
    12961 tggtccggta cgtttgctgg cgaagagcgg cggcaagtcg ggtgacttta aggtggaagc
    13021 ggatgattaa agttcttttt ttcgcccagg tgcgcgagtt ggtgggaaca gatgcaaccg
    13081 aagtggctgc ggattaccca accgttgaag cgttacgcca gcacctggct gcgcagagcg
    13141 atcgctgggc gctggcgctg gaagatggca aattgctggc tgccgtcaac cagacgctgg
    13201 tgagttttga ccatccgctg actgacggcg acgaagtagc tttcttcccg ccggtaaccg
    13261 gaggttaaga tggcagaaac caaaattgtt gtcggcccgc agccgttcag cgtaggagaa
    13321 gagtacccgt ggctggcgga gcgtgacgaa gacggtgcgg tagtcacctt caccggaaag
    13381 gtgcgcaacc ataacctggg cgacagcgtc aaagcattaa ccctcgaaca ctatccgggg
    13441 atgactgaaa aagcactggc agaaattgtt gatgaagcgc gtaaccgctg gccgctgggg
    13501 cgcgtcacgg tgattcaccg catcggggaa ttatggccgg gcgatgaaat cgtttttgtc
    13561 ggtgtcacca gtgcgcatcg cagcagtgcg tttgaagccg ggcagtttat tatggattat
    13621 ctcaaaaccc gcgcaccgtt ctggaagcgc gaagccacgc cggaaggcga ccgctgggtt
    13681 gaagcccggg agagcgatct gcaggcggca aaacgctggt agttttttgt tagccggata
    13741 aggcaccgct gcgtccggca atttcaccgc tcagtacatg cgtttacgct gcgtcgatgc
    13801 acagcctcat cattttgcag tatccttaag atattcctta tatcttcagg agatcgtcat
    13861 ggacagattc ccacgttctg attcaatcgt acaaccctgg gctggcttgc aaacttatat
    13921 ggcgcaagtc tatggctgga tgaccgttgg cttgttgctg accgcatttg ttgcctggta
    13981 tgcggctaat tccgcggccg tgatggagct gttgttcact aaccgtgtct ttttaatcgg
    14041 tctgatcatc gcgcaattag cattggttat tgtgttatca gcgatgattc aaaagctgag
    14101 cgcaggtgta acgacgatgc tctttatgct ttattcggcg ctgacgggtc ttacgctttc
    14161 cagtatattc attgtctata ccgctgcttc tatcgccagt actttcgtcg ttactgccgg
    14221 gatgttcggc gcaatgagcc tgtacggtta caccacgaag cgcgatttaa gtggctttgg
    14281 caatatgctg tttatggcgt taatcggcat tgtgctggca tcgctggtca acttctggct
    14341 gaaaagcgaa gcattgatgt gggcagttac ctacatcggc gtgattgtct ttgtcggatt
    14401 gacggcgtat gacactcaga aattgaaaaa tatgggtgag cagattgata cccgcgacac
    14461 gtcgaacctg cgcaaatatt ccattcttgg tgcgttaacc ttgtatctgg acttcatcaa
    14521 cctgttcctg atgttgttac ggatcttcgg caaccgccgt taattctttt tcaccatcac
    14581 tatcccggcc atgtgtcggg atttttttct tactaaaaac gacacttaac aagtaccagg
    14641 tataaaatat gccttatgag cgagcggaat cgttttccgt atccataaat acaaaagtat
    14701 tttgtatgcg tggttttaaa tttattgatg gtgaatatta atgttttttc taaggacaac
    14761 taaaagaatg gaatcatact cgcaaaacag caataaatta gattttcagc acgaggccag
    14821 gatattaaac ggtatatggc tcattaccgc tttaggtttg gtggcaaccg caggactagc
    14881 ctggggagct aagtatctcg aaattacggc aaccaaatat gattcaccac caatgtatgt
    14941 cgccataggg ttattattac tttgtatgta tggcttaagt aaggatatca acaagataaa
    15001 tgccgccatc gcgggcgtaa tatatctgtt tttactctct ttggtggcga ttgtcgttgc
    15061 aagtttagtt cctgtatatg ccattatcat cgtgttcagc actgcgggcg cgatgttttt
    15121 aatcagtatg ctggccggtt tattatttaa tgttgatcct ggttctcacc gttttatcat
    15181 tatgatgacg ttgacagggt tggccctggt aatcatcgtg aatgcggcat taatgagtga
    15241 acggcccatt tgggtaataa gttgcttaat gattgtgtta tggccaggca ttatctcgca
    15301 tggacgaaat aagctccttg aactggcagg aaaatgccat agtgaagagt tgtggagtcc
    15361 ggttcgttgc gcttttacag gtgcattaac actctattac tattttatcg gcttctttgg
    15421 gatacttgcc gcgatagcta taacgcttgt ctggcaaagg catacgcgtt tttttcatta
    15481 gtcgtaaatg cagatactaa agaacttaac taaacttcac atcgccgctt catttttcgc
    15541 ccgtagcttc ttcgcttggc tttccagtag caggtaacag atcagcgcca gcagcagcgg
    15601 gataaagtaa tacaggacgc gataagcgag cagggcggcg ataatgctcc ctttcgaggt
    15661 atgctcccca gccagtagtg cgataaacac cgcttccagc acaccgatcc ccgccggaat
    15721 atgcacgatg acgccagcaa tactactaac cagtaacacg cccagtacaa agaaatagtt
    15781 cacgctttga ccaagtaaca gccagataat cgcccccatt accatccagt taacgctgga
    15841 aatcagcatc tgagcgaggg cgaatttcca tgaaggcagc accagctttt gccctttgat
    15901 ggtcatatgg cggtgcttcg cgaaagcgca aaaccacaaa taaaccgcga taatcatcag
    15961 taagccaatg ccgagaatac gcagcgtagt ttgatcgaca taccagtaat ccggcaactc
    16021 caccacgcct gcggtaaaga taatccctgc cagtaaaata tagcccagcc agttggtggt
    16081 aatactgagc gaggaaatcc gcgtaatagt gctgcccggt aaccctagtc gggagtacaa
    16141 acgatagcgc ataccaatgc cgccgaccca ggtactgagc gtcaggttga aggcgtagca
    16201 gataaacgac accagcatca cctggcgctt cgccagtttg tgaccgcagt aaaagcgggc
    16261 gagcaggtca tagcagccgt aaatcagata gctgacgacc accagcccga ccgcactaag
    16321 cagcgcaacg cgattgtagt cgcggatgac cttccagacc tcttcccagt ccactttttt
    16381 ggcgtagacc accagcaaca caatcaccgc gataaaaaac agccaggtga ggatcttctt
    16441 tgctaagcgc cagcgcgggt gtgatttact catcaggggt ttacccccgt gttttcagtt
    16501 tctacccgat cctgcgtttc cattgtcggt tgtgcggggg gatcaacctg cgtcagacgt
    16561 ggtgtgtgtg ccggaagcca gccaaccagc gccgggaagt ggcgtaaaaa gtggaacgcc
    16621 agcacgcttt tggtcaggtt ccaccaggtg cgtttgggca gcatggtttc atccacctgc
    16681 tgacaatctg cggcaataat gccgttcaga ttatcgcgca gcgtctggtt aaaatgacga
    16741 tcgtggatga tgacatttgc ttcgagattc agtgacaaac tgagcggatc gagattactg
    16801 gaccctactg tcgcccagtg atcgtccatc aatgccactt tgccgtggag cgggcggcgg
    16861 cggtactcaa aaacctgaac gccgccttta accagatagt tatacagcaa gcgcgcaccg
    16921 actctgacaa tcggcatatc cggttcgccc tgaatgatca gtttgatccg caccccgcgc
    16981 cgtgccgctt tacgcaaggc gtgtaaaaat cgatagccgg ggaagaagta ggcgttggcg
    17041 ataatcactt cccgctgcgc ctgagtgagc attttcagat aatggcgttc aatatcatcg
    17101 cgatgttctt cgttatcgcg ccagaccagc aatacctgcg cttctcccgg ctggcggttc
    17161 tcttccgctt tgtgatgacg tcgccaccag cgtcgtgccg cgctctgtcc aggcaggttt
    17221 tccagctcaa actggagaat atcttcgaca atcggccctt caaggcgtac cgcgtaatcc
    17281 tgtttagcct ctggaccgta gctggacata tgctcggcgg agtaattcag cccgccaata
    17341 aaggcgatac gcgcgtcgat caccacaatt ttgcgatgca tccggcgaaa cacattggtg
    17401 cgcataccaa aaaggcgagg gcggggatcg tagtagcgga acactacgcc agctgccgtc
    17461 agttcattga caaactcatc gctgagatcc ggcgaaccgt agccatccag caagacttcc
    17521 gctttaaccc cgcgttgcgc tgctgccagt agtgccgcat gcagctgttt gccgacgtta
    17581 tcctcaaacc agataaacgt ttcaagaatg atgcgttctt gtgcctcgcc aatcgcctta
    17641 aacaccgcgg gataatattg ctcgccgttt tccagcaact ggatcttatt gccttcgcgc
    17701 cagctacatt tcataaatga atctccgcac ttaaaggggc atgatcagaa aggtgtcgcc
    17761 atgtccgcag cggcaacgcg gttggcgcgc tggcgctggc atttttgacg tagatcctgt
    17821 ccagtcgtag tagaggaaat tgcaccggaa acgtgcgcgc cgggcgtccg tgggcgcggg
    17881 taaaaatctc atccagtccg gcctgcactt ttaacggatg attagctttt tgccgccagt
    17941 cattgaaatc acccgccacc aataccggtt cgccgtccgg tagctcattc acccattcgg
    18001 cgagcatcgc aagctgcgcc tgacggtgcg cctcacgcag gcccagatgt acgcacatca
    18061 catgaatcgc ttttccggtc atcggcggca caatgcggca gtagagcacg ccgcgctttt
    18121 ccgcaccatc gaccgaaaca tcgcgattct cataatgttc aatgggataa cgcgacagta
    18181 cggcgttgcc gtgatgccct tccgggtata cggcattgcg accgtaggca aaatcgctcc
    18241 acatagtgtc ggcgagaaac tcgtagtgcg aggtatcggg ccagttttcc acatgcagcg
    18301 gatgaacttc gtgcgcgccc atcacttcct gcaggcaaac aatatcggcg ctgacggtac
    18361 gcacggcatc gcgaagttcc ggcaaaatga agcgtcggtt aaacgcggta aagcctttgt
    18421 gaatgttgat ggtgagcacc ttgaacgaaa attgttgtgt ttgatcgggc ataattttcc
    18481 tgtctttgcc tcttatctca ttgaaatagt gtagtcggcg tcacaaaaag gtgcggtctt
    18541 acggaatttt ccgtaaagtt cggtactctg agtaagtaga gataaattct tcaggagaga
    18601 agccatgaag tggcaacaac gtgttcgtgt cgcaacgggt ctaagttgct ggcagattat
    18661 gttgcattta ctggtagtgg cgctgctggt ggtgggctgg atgagtaaga ctctggttca
    18721 cgtcggcgtg ggattatgcg cactgtattg tgtcacggta gtgatgatgc tggtgtttca
    18781 gcgccacccc gagcaacgct ggcgtgaggt ggcagacgtg ctggaagagc tgaccacgac
    18841 ctggtatttt ggcgcagcgc tgattgtgct gtggctgttg tcccgcgtac tggaaaacaa
    18901 ctttttgctg gcaattgcag ggctggcgat ccttgctggc ccggcggtag tgtctttgct
    18961 ggcgaaagat aagaagttac atcaccttac gtctaaacat cgcgtacgcc gctgaccctg
    19021 tcgtggccgt tatcaccagt agcggccaca aactattcca cacaatatcc agactcgcat
    19081 ccttcaaata aatctgcttg gtaatgtccg taaagtggcg aatagggtta atccacgtca
    19141 gattttgcag ccacactggc atgttttcca ccggcgaaac gtaaccggaa aggagaatgg
    19201 cgggcatcat aaaaacaaac acgccgataa acgcctgctg ttgtgtcgag cagagtgatg
    19261 aaatcaacag accgaatccc accagcgata aaccgtaaat caccatcgta aagtagaaca
    19321 gcgctagcga tccggcgaag gggatttgat acgcccagat accaatcgcc agcacaatgg
    19381 tggcctggaa ggtggcgaca attaacgccg gtacggcttt gccgatgaag atctgccagg
    19441 tggtgagcgg cgaaaccagt agctgatcga gcgtaccttg ttcacgttcg cgggcgacgg
    19501 aaagtgaagt gacgatcatt acgccgatag tggtgatcat ggcgatcagc gacggcacca
    19561 caaaccattt gtagtcgaga ttcgggttat accagttgcg taccaccagc tcgctgttgt
    19621 tgggtttcgg ttttccttcc agcagctcct gctgataatt tttgacgatc tgttgcaggt
    19681 agttggcggc aatttgcgca ctgttggagt tacgcccgtc gaggatcaac tgcaacggcg
    19741 cggtctggaa ggtatccagt ttgcgcgaga agtcagccgg gaaacgcacc agtagtaacg
    19801 ccttttgtgt gtcgatggtc gggcggatct cctgtgggct tttcagcagc agcacatgtg
    19861 taaaggcgct ggcgcgggca aaacgttgag taagttccac cgaatgctcg ccgttatctt
    19921 catcgtagat ggcgatggtg gcgttagtca cttccagcgt ggcggcgaac gggaacagga
    19981 tcacctgaat tagcacgggt aaaatcagaa tcgcgcgggt ttgtggttcg cgcagcaacg
    20041 actgcaactc tttgcggatt aacgtccata agcgatgaaa catgctcttc tccctaatcc
    20101 agccgacgtt tggttttcag ccacgtcagg ccgataaaca tcaccgccga agcgatcaaa
    20161 aacagcacgt ttaccaccag caccactgga atattcccgg cgaggaacag gctttgcagg
    20221 gtgctgacga aataacgagc gggaataatg tacgtcaccg cgcggatcac cgcgggcata
    20281 ctgtcgatct gaaaaataaa gccggaaagc ataatcgacg gcagaaaagc ggcgttcagg
    20341 gcgacctgag cggcattgaa ctggttgcgg gtaatcgtgg aaatcagcag ccccattccc
    20401 agggtactga gtaaaaacag gctggagata aaaaacagaa tcagcagcga accgcgatac
    20461 ggcacgccga gaataaacac tgacaccagc atacacagca acatcgccag catcccgaga
    20521 aagtaataag ggatcagctt acacagcagc agttccgtgc gggtaatctc cgtagagagc
    20581 agagcctcca tggtgccgcg ttcccattct cgcgccacca ccagcgaggt gagaatcgcg
    20641 ccgatgaccg tcatgataat ggtcaccgca cccgggataa taaagtgctg gcttatcgcc
    20701 gccgggttaa accagtagcg ggtttgtaca tcaataagcg gttcaaaagt ctgcccgttg
    20761 tcctccgctc gctgcatttg ccagatctgc cagatccctt cgacataccc ctgcacaaag
    20821 ttagcggtat tcggctcact gccgtcggtg atcacctgaa tcggtgcggt ggcgttggcg
    20881 cgctccatct gttcggcgaa atccacggga ataaccacca gaccgcgaat tttccccgac
    20941 tgcattttgg cgatcagttc ctgacggtta tcgctgatgg tggcgtcgat gtagggcgaa
    21001 ccggtcatgg tgtgggtgaa atccagcgcc gcttcgctac gctgttccag caaaatcccg
    21061 acccgcagct tgctggagtc gaggttaatg ccgtaaccaa aaataaacag cagtagcagt
    21121 gggatcacta ccgcaatcag ccagctactc ggatcgcgaa cgatctgccg cgtctctttg
    21181 acgcacagcg cccgcacgcg atgccaggaa aggatcgggt tactcattgc tatgctcctt
    21241 atcccagtcg tggatcaact gaataaaggc ttgctccatg gtgggatcgg gttgctcatc
    21301 gttagctgac tgtgctttca aatcgtccgg cgtaccgctg gcgattaatt tcccacggta
    21361 caccaggccg atgcggtcgc aatattccgc ttcatccata aagtgggtgg tgaccatcac
    21421 cgtgacgcct ttctctacca tgctgttgat gtgcagccaa aattcacggc gggtgagggg
    21481 gtcaacgccg gaagtcggtt cgtcgagaaa gagaatatcc ggttcatgca tcagcgaaca
    21541 ggccagcgcc agccgctgtt taaaacctaa tggcagttca tcggtggcgt gtgaggcaat
    21601 acttttcagg ccaaatgcct cgctcatacg ggagattttt tcgttctgcg cccgaccgcg
    21661 taagccatac acaccagaga aaaagcgtaa attctgttcg accgtcaggt tgccgtagag
    21721 cgaaaatttt tgcgccatat agccgagatg ctggcgcgct ttaccggaac tctctttcag
    21781 atccatcccc agcaccagcg cctggccgga agtcggctcc agcaaaccgc acatcatctt
    21841 aaaggtggtc gatttacccg cgccgtttgg ccccagcaaa ccaaaaatct ccccacgttt
    21901 aacggcaaag ttgacgtgat cggtggcggc aaaatcacca aatttcttgg tcagttcttt
    21961 cgcttcgatc accgtctcgc cgggtgtgcc ttctaccgta tgcaatattg cacccagcgg
    22021 cgattctgag gttccggcac cgcccagcaa atcaataaac gcatcttcaa aacgcggcgt
    22081 agtttcgttg atgttgattt ccggcatccc gtcggcatgg cgaatatcgt ctggtgtggc
    22141 ctcttttttg aggatcagac gtaccgattt cccctgaatc atgccatcgc tgacctgcgg
    22201 cagtttcaag gcgcgttgca acagtttgcg gttgccctcg tgtggactgg tcatcagaaa
    22261 gctgcgtccg gccatggttt gtgtcagggc ttttggttct ccctgataca gcaactcgcc
    22321 ttcgttcatc agtaacacgt cacggcactg ctcggcttcg tcgagatacg aggtactcca
    22381 gaggattaac atcccttcgc ccgccagctc atgcaccatc tgccacagtt cgcgccgtga
    22441 gatagggtca acgccgacgc cgggttcatc gagcagcaac actttcggtt cgcccaccag
    22501 ggtacaggcc aggccgagtt tttgtttcat cccaccggag agcttgcccg ccaggcgtcc
    22561 ggtaaacggc ccaagagacg taaactccag caggcgagca aaagtttgct tacgtgcctc
    22621 gccggtgacg ctgcgcaaat ccgcgtacag attgaggttc tccatcaccg tgagatcttc
    22681 atacagacca aatttctgcg gcatatagcc gagcacggcg tgcagcgcgc cgtcgttttt
    22741 gatcggatca aagccaatca ccgtggcact gccgctgtcg ggtttcagta atcccgccaa
    22801 catccgcatc agtgtggttt tacctgcacc gtccggcccc accaaccccg tcacataacc
    22861 ggcgtgaatg gtacaatcga gcggcgcgac ggcgggcttg tccatgcccg gaaagcgttt
    22921 ttccaggccg ttcagcgtga taacggcatc attcatgtcc tgcctcgtca ccgaattgta
    22981 ccgtcactgg cattccctgg cgtaacgcat catcggcgtc ggtcaccaca atacgcaggc
    23041 gatagacgag gtcggtacgc agatccggtg tttcgacggt tttcggggta aattcagcag
    23101 tcggcgaaac gaaaccaatc tgcccgtgat acggcttgtc cgggcgacca tcggtataaa
    23161 gcagcacttt gcgccccggc tgggcctggt caagattccg ttcatcaaca taagcgcgca
    23221 cccacaccgg acgcgttagt gaaacggtaa acaccgtgcc accttcattg agaaccgtgc
    23281 ctggctctac cgcgcgcgtt aacagcgtgc catcagacgg tgctatcaac gttgagtcct
    23341 gtaaattcag ctccgcctgc gccagttgcg cctgcgcctg ttcgaggctg gctttcgcct
    23401 gagcgatgtc ctgttcacgg ttaccggaac ggtactgacg caatttatcc tgtgctgatt
    23461 tcagcgttgc ctgtgcctgg tcgcgcgagg agcgggcatt ttccaggtca tttgccgaaa
    23521 tggtgcggct tttccacagt ccttgctggc ggttatagaa gttctgcgca tagtcatagg
    23581 cggcttgcgc ctgtttcacc gctgcggcgg cctgagcgat ttcttcatcg cgatacccgg
    23641 caagcatcag atcatactgc gcctgtgcca ccgagacgcc cgctttcgcc tgcatcaggg
    23701 caatctcata cggcttgtga tccagttcgc ccagcacctg gcccgctttg atagcatcac
    23761 cttcgtccac cgccagcgat tcaacgcgcc ccccaacacg gaaactaaga ttcaccgtac
    23821 gaatatccac gttgccatac agcgtcaggc cgttatcctg gcggctttga taccaccagt
    23881 agcctccggc aaccacggcg gcaagtacca ctaccgccag tccgatcacg acaggttttt
    23941 tcatcactac aaactccttt gcgataatcc ttgcaaaatc aggtcgatat gacaggtcac
    24001 cgtctggttg atcagttcgg tcttttcttc atcgaacgcg gtccagccgg tacgtaacag
    24061 aattgtttct ttgccaagac ggaacgccag aatctcacca atcaacgcat gggtatgaag
    24121 gatcatgcgg gtgtcattag cgtcgcagcc ggtccaggcg gcaatcaggc gtgtcaggtg
    24181 gctgtgtagc ggactaatca cctgttcgtg caccaggtgg taggctgccg tgggagagag
    24241 ctgctcacgg gagataaact tgctgaggtt gacggtgtca tcctgggtga gcagcttaat
    24301 catgttcctg caggcgcgaa ggatcagttc acggatggca gcccgatcag gctgtggttg
    24361 ggcgaacaag cgttcggctt cctcggcatg cggacggaac tgctcgccaa taaaatcggc
    24421 aatccactgg gcgcaggcga ggtacaaatc ttctttcgaa ccgaagtagt aggtgatggc
    24481 agcgatattc tgcccggcct gggcggctat ctcgcgggtg gtggcgttca ttccatattc
    24541 accaaactgc gccagtgcgg cagcaatcag ctgttttttc gcctgttcac ccttgattgt
    24601 catggcagga ttattcatcg cacagtccat tcttaatcaa atgattgatt aagattatga
    24661 ctccatatgg gagttgtcca gtatggctaa gaattttagc tacgccagtc acagggataa
    24721 tttatgcgct gcgtcacaaa aactgctaca ctccgctccc tcatgacatt gtggtttttg
    24781 tcattttcct tttcagtatc tccctgaaaa ctacaccggt aacggtcggg gcggttcgga
    24841 gtagttatgt ctttcgattc tttgggttta agccctgata tcctgcgcgc cgttgccgag
    24901 cagggttacc gtgaacccac ctctattcag cagcaggcga tccctgctgt gctggaaggc
    24961 cgcgacctga tggctagcgc ccagaccggc accggcaaaa cagcgggctt tacgctgccg
    25021 ctgttgcaac acctgatcac tcgccagccg cacgccaaag ggcgtcgtcc ggtacgtgcg
    25081 ctcattctta ccccgacccg tgaactggcg gcacagattg gcgaaaacgt ccgtgattac
    25141 agcaaatacc tgaacattcg ttcgctggtg gtgtttggcg gtgtcagcat taacccacaa
    25201 atgatgaagt tgcgcggcgg cgttgatgtg ctggtggcaa cgccgggacg tttgctggac
    25261 ctggaacatc aaaatgcagt gaagctggat caggttgaaa tcctcgtcct cgatgaagct
    25321 gaccgcatgc tcgacatggg ctttatccac gatatccgtc gcgtgttaac aaaactgcca
    25381 gcgaaacgcc agaacctgct gttctctgcg accttctctg acgatattaa agccctggcg
    25441 gaaaaactgt tgcacaaccc gctggaaatc gaagtggcac gccgcaatac cgcgtctgat
    25501 caggtgaccc agcacgttca ctttgtcgat aagaaacgca aacgcgaatt gctgtcgcac
    25561 atgattggta aagggaactg gcagcaggtg ctggtgttta cccgtaccaa acacggcgct
    25621 aaccatctgg ctgagcagct caataaagat ggcatccgta gtgtggcgat ccacgggaat
    25681 aaatcgcaag gtgcgcgtac ccgtgcgctg gctgatttta aatcgggtga cattcgcgta
    25741 ctggtggcga ccgatatcgc tgcgcgcggt ctggatatcg aagagttgcc gcacgtcgtc
    25801 aactatgaac tgccaaacgt accggaagat tatgtccacc gtatcgggcg taccggtcgt
    25861 gcggctgcta ccggtgaagc gttgtcgctg gtgtgtgttg atgaacacaa actgctgcgt
    25921 gatatcgaaa aactgctgaa aaaagagatc ccgcgcattg cgattccggg ctatgagccg
    25981 gacccgtcaa tcaaagccga accgatccag aacggtcgcc agcaacgtgg cggcatcagg
    26041 ctgatgaaca aacgcaaaac tgcctgatgc gctacgctta tcaggcctac gtgaactctg
    26101 caatatattg aatttgcatg cttttgtagg ccggataagg cgtttacgcc gcatccggca
    26161 tttcacaaca agcacttgtc agcaatttga gaacacggga aaaaatttat ctgttttacc
    26221 gcccataagc caccgagacg ttacttctct atagccaatt gttcgcgcaa ctccattaaa
    26281 aggtagccca gtcgattctt gcccttacca ttaccaccgt ctccccagta agcatcgttt
    26341 tccgtatgct caaccagttt tgcgggcgcg gttgccagca agagcacacg cagttctgca
    26401 tgctgttcga atttagcgcg aagtgcttta cgcatcacct gttctttaac cgactcccag
    26461 tttttacgaa gtggcttgga acgattacgc cccatgcgag cggcgaccat gggggaagaa
    26521 acccgacgga tctcttcgcg gtatttttca tcaaggaatt tttgtgcctg aaaatagtgt
    26581 tcggaggtgg gccaggtatt cccgtcaacc ttgatgggcc tggcggcaaa gttagaaaaa
    26641 tcaccgtagt catcgctggt gctgtagaaa tttatgatgg tgtcttgcat gacgtgttgg
    26701 atcctttgtg ctcgaacggg cattaaaccg cattatgttg gtggttattg cgagccgctt
    26761 tccagaaaca gaaaaaccat tacccctgaa aaccgaaaaa tgccacaata ttggctgttt
    26821 atacagtatt tcaggttttc tcatggcatt aaccgccgcg cttaaagcgc aaattgccgc
    26881 ctggtataag gcgcttcagg aacagatccc cgactttatt ccccgtgcgc cgcagcggca
    26941 gatgattgcg gacgtcgcca aaacgctggc cggagaagaa gggcggcatc tggcgattga
    27001 agcccccacc ggcgttggga aaacgctctc ctatttgatc cccggcatcg ccattgcccg
    27061 cgaagagcaa aaaacgctgg tggtgagtac cgccaacgtg gcgttgcagg atcagattta
    27121 cagcaaagat ttaccgctgc tgaaaaagat cattcccgat cttaaattca ctgccgcttt
    27181 cgggcgtggg cgctacgttt gtccacgtaa tctgacggcg ctcgccagca ctgaacccac
    27241 gcaacaggat ctgctggcgt ttcttgacga cgaactgacg ccaaataatc aggaagagca
    27301 aaaacgttgt gcgaagctga agggcgatct cgacacttat aaatgggatg gtctgcgcga
    27361 tcatacggat atcgctatag atgatgatct ctggcgtcgt ttgagtaccg acaaagccag
    27421 ctgcctcaac cgtaactgtt actactatcg tgaatgcccg ttttttgtta ctcgtcggga
    27481 gattcaggaa gcggaagtgg tggtggcaaa ccatgcgctg gtgatggcgg cgatggaaag
    27541 cgaagccgta ctgcctgacc cgaaaaatct gctgctggtg ctggacgaag gtcatcacct
    27601 gccagatgtg gcccgggatg cgctggagat gagcgctgaa atcaccgtgc cctggtatcg
    27661 actacagctg gacttgttca cgaaactggt cgctacctgc atggagcagt ttcgcccgaa
    27721 gaccatccca ccgctggcga tccctgaacg tttgaatgcg cattgtgaag agttgtatga
    27781 gcttatcgcc tcgttaaaca acattctcaa tctctacatg cctgccgggc aggaggcaga
    27841 gcaccgtttt gcgatgggcg aactgcctga tgaattgctg gagatctgcc agcggctggc
    27901 aaaactcacc gagatgctgc gtggtctggc ggagttattt cttaacgatt taagtgagaa
    27961 aaccggcagc cacgatattg tgcgtctgca tcggttgatt ttacagatga accgcgcgct
    28021 ggggatgttt gaggtgcaaa gcaaactctg gcggctggcg tctctggcgc aatcttccgg
    28081 tgcgccggtg accaaatggg cgacgcggga agagcgcgaa gggcagctac acctctggtt
    28141 tcactgcgtg ggaatacgtg tcagcgatca gctggaaagg ctgctgtggc gcagtattcc
    28201 gcacattatt gtcacctccg caaccttgcg ttcgctgaat agtttttcgc gtttgcagga
    28261 gatgagtggt ctgaaagaga aagcgggcga ccgttttgtg gcgctggatt ccccctttaa
    28321 ccactgcgaa cagggcaaaa ttgttattcc ccggatgcgc tttgagcctt ccatcgacaa
    28381 cgaagagcaa cacattgccg aaatggcggc ctttttccgt gaacaggtgg agagcaaaaa
    28441 atatctcggt atgttggtac tgtttgccag cggacgggcg atgcagcgct ttctcgacta
    28501 tgtgacggat ttacgcctga tgttgctggt gcagggcgat cagccgcgtt atcgcttagt
    28561 tgaactgcac cgcaaacgcg ttgccaacgg tgagcgtagc gtgctggtgg gcttacagtc
    28621 atttgccgaa gggcttgatt tgaaaggtga tctgctcagc caggtgcata tccacaaaat
    28681 cgcttttccg cccatcgaca gcccggtggt gatcaccgaa ggggaatggc tgaaaagcct
    28741 caaccgctat ccgtttgagg tgcaaagcct gccgagcgcc tcgtttaacc tgattcagca
    28801 ggttgggcga ctgattcgaa gccacggttg ctggggcgaa gtggttatct acgacaaacg
    28861 cttgctgaca aaaaattatg gcaagcgact actggatgca ttaccggtat ttccgataga
    28921 gcaaccggaa gtccctgaag gtatagttaa aaagaaagaa aaaacgaaat ccccacgccg
    28981 tcggcggcgt taatgatgtg agtcaggtaa ggagtcgtaa atggactatc gcaaaatcat
    29041 taaagagatc gggcgcggga aaaaccacgc gcgcgattta gaccgggata ctgcgcgcgg
    29101 tctgtatgct catatgctca atggtgaagt ccctgacctt gagttgggcg gcgtattgat
    29161 tgcgctgcgt atcaaagggg aaggggaagc agagatgctc ggcttttacg aagccatgca
    29221 aaatcacacc atcaagctga cgccgcctgc aggcaagccg atgccgattg tcatccccag
    29281 ttacaacggc gcgcgtaaac aggctaacct gacgccatta ttggcgattc tgctgcataa
    29341 actcggtttt cctgtggtgg ttcacggagt tagcgaagat ccaactcgcg tgctgacaga
    29401 aaccattttc gaactgatgg gtattacacc aacgttgcac ggcgggcagg cgcaggcgaa
    29461 gctcgacgaa catcaaccgg tgtttatgcc tgtcggcgcg ttttgcccgc cgctggaaaa
    29521 acaactggcg atgcgctggc ggatgggcgt gcgtaacagt gcacataccc tggcgaaact
    29581 ggcgacgcca tttgccgaag gtgaggcact gtgtctttcc agcgtttcgc atccggaata
    29641 cattggacgc gtcgcgaagt tctttagcga tatcggcggg tgggcgttac taatgcatgg
    29701 tactgaaggc gaagtgtatg ctaatccgca gcgctgcccg cagatcaatc tcattgaccg
    29761 tgaagggatg cgggtgctgt atgaaaaaca ggaccctgct ggtagcgagt tactgccaca
    29821 agcaaaagat ccggaaacca cggcgcagtg gactgagcgt tgcctggctg gcagcgaagc
    29881 gattcccgaa tcgctgaaaa tccagatggc ttgctgcctg gtggctacgg gtgaagcggc
    29941 aactatcagc gatggcctgg cgcgcgttaa tcaggcattt taatttttat ctcagccgga
    30001 tgagccacgt catccggctt ttaccccacc gcaatatgaa attcctgcat ctttattgac
    30061 cttcccgcgc ccggcgtgca gcataaaaaa acaacaaaca cataacataa acaggagtta
    30121 accatggaaa gtggtcatcg ctttgatgct cagacgctgc acagttttat tcaggctgta
    30181 tttcgtcaga tgggtagcga ggaacaagaa gcgaaattag ttgccgatca tttaatcgcg
    30241 gcaaacctgg cagggcatga ttctcatggt attggcatga tcccaagcta tgtgcgttcc
    30301 tggagtcagg ggcatctgca aattaaccat catgccaaaa tcgttaaaga ggcgggggcg
    30361 gcagtcacgc tcgatggcga tcgcgcattc ggtcaggtcg cggcacacga agcgatggcg
    30421 ctggggattg agaaagcgca tcagcacggt attgctgccg tggcgctaca taactcgcat
    30481 catatcggcc gtatcggtta ctgggcggag cagtgttcag cggcggggtt tgtctctatc
    30541 cactttgtta gcgtggtcgg tattccaatg gtcgcgccgt tccacggtcg cgacagccgc
    30601 tttggcacca atcccttctg tgtggttttc cctcgtaaag ataatttccc gctgttgctt
    30661 gattacgcca ccagcgccat tgcatttggc aaaacccgcg tcgcctggca taaaggcgtc
    30721 cccgtgccgc caggttgcct gattgacgtt aacggcgtgc cgacgaccaa tccggcggta
    30781 atgcaggagt cgccgttggg gtcgctgttg acctttgccg aacataaagg ctacgccctt
    30841 gcagcgatgt gtgaaattct tggcggggcg ctttctggcg gtaaaacgac gcatcaggaa
    30901 acgttgcaaa ccagccccga tgccattctt aactgcatga ccactatcat catcaacccg
    30961 gaactgttcg gcgcgccgga ttgtaacgcg cagaccgaag cctttgccga gtgggtgaaa
    31021 gcctcgccgc atgatgacga taagccgatt ttgctaccgg gcgagtggga agtgaacacg
    31081 cgtcgcgaac ggcaggagca ggggattcca ttggatgcgg gaagctggca ggccatttgt
    31141 gatgcggcgc ggcagattgg tatgtcggaa gagacgttgc aggctttctg tcagcagtta
    31201 gccagctaaa aaaaagcccg tccagtggcg gacgggcaaa caagggtaac ataggatcaa
    31261 tgagggttag agcatatgcg tctgtcggca aacagacagg gaaatacttg tgctggacgt
    31321 agcgtaaacg cctgatccgt ccaacggttc ggattttgta ggcctgataa gacgcggtag
    31381 cgtcgcatca ggcatctgtt gccggatgcg gcataaacgc cttatccgtc ctacgaatta
    31441 cttataaatt accgcagtac cgcttaattt attgttgttg gtggcggaag tgatgctgta
    31501 tccactggca ccggctgccg ccgctttctc agccagtttc gcttccaggg catcgagggt
    31561 ggatgcgcca tcggcagaaa ccacgccgat tttattcatg ttctgtgcct gggatgccgt
    31621 taccggttcc gccgcgaaca cgccaaatga cagagttgaa agagccatag cagcaacaac
    31681 agtattgata gttttcataa ttaatctctc gcaggtgatt tttgtataag tgacgttgtt
    31741 tcgtcgatgt gatgagtatc acgttttttt acgagagata aaatcgaaga gaattgaccg
    31801 ccttgttcaa ataaattgat tgatatctaa catactgata aaccatttat ttgttgtact
    31861 tatggacttg ttttatgccg cgtggcagtc acagcagcgt taaaggcacg gaatgccacg
    31921 caaaatacaa attatactcg ctatgcatct cgccagttgt agttaacgta actgtctgtc
    31981 tttcgaacct ctgcgattat atcctgtata cgctggtttt tgtaaatctt tctcctgctg
    32041 acaatgaata cataagcgca cgccaggaat ggcttcccga cgggcttgcg ggatgggggc
    32101 accgcactct tcacattcat acaggctttc gccacgcgga atttcacccc gagcgcgggc
    32161 aatcgcatct tcaattgtac tgttgatctg ttcgttgacg gcgtcatcgt tagcccaagc
    32221 ggatgccata tcgtcctccc catatcaatg cttgtacagt taagtgtagc taatataggg
    32281 acgaacctgg gcagttcaag cgtcagatct ccgaccattc ctgcagcaga ttatgataaa
    32341 gattgagcag cgacaggatc tcttcatttt caccatagcg gcttttcagc gactgaatat
    32401 ttttgtccag ttcaaacagc atggcgcgct ttttatcatc gcggatcatc gactggatcc
    32461 acataaatga tgccactcgt acgccgcggg ttacgggtgt cacgcaatgc aggctgctgg
    32521 agggatacaa caccagatca cctgccggga gttttacccg atgttgtcca aaggtgtcat
    32581 taacgaccag ttcgccgccg tcgtagcttt gtggatcgct taaaaacagc gtggcagaaa
    32641 ggtcagtgcg catccagccg ttttgtggat ggctgcgtac tgcgccatcc acatggaaac
    32701 cataggtttc attgttctga tagcgattaa acagcggcgt ggaaagggta cgcggcaagg
    32761 cagcggcaaa gaacaatgca tgttggttaa ccgcgttcag cacctcattt tgcagtgcgg
    32821 cgtataacgc gctgcgggtg tctacttgct gattgttctt aacttgtgca ccttgtgctc
    32881 cggtggtgac gcgtccatcc acccattcgg cttgttccag ttgttcgcga aaatgggcga
    32941 cgtcctgtgg cgataacacg ccgggaatgt ggtacatcat acaagttctc caaaaagtgg
    33001 ggcctgcgcc ccacatctga atcagaaatg catattggct gtgagcaaga aggttcttgg
    33061 ctcgcccggg tgataacggt agccgctctt gttgattgag gcgacgtaat cggtatcaaa
    33121 caggttgtaa acgtttagct ggaagtcgag attgcgatta acccgatacc ccagtttggc
    33181 atcggcgacc cagtaacctt ctgtaaatgc tggcgttccc accgcgccgt ctgaaccttt
    33241 atgtatactg ccgatatagc gtgcgcccgc gccaacagag atatcatcgg ttgcctgata
    33301 ttggctccat aaggtgaagg cgtgctccgg ggtatacggc agcgatgagg aaccatcctg
    33361 ggcaacatct ttgccgtttt tgatggttgc tttttgctgg gtatagccgc caatcacctg
    33421 ccacgcggga gtgatattcc ctgccacgga tatctcatag ccttcgacgc gtttcttacc
    33481 gtattgcgag taagtcccgt catcattttg ctcaacttca ttttcgatat cagtacggaa
    33541 cagcgcggcg gtgagcaaca ggcgtttatc cagaacctgc catttggtgc caatctcgct
    33601 ggtgttggct ttttgcggtt taaaatcggt gcggttggca ctgttaccac tgccagactg
    33661 cgcaagggcg aagttgttgc cgcccggagg ctgctgggaa acggcatagt taatatagac
    33721 attgccgttt tccgtcaggt ggtacagcgc cccggctttc cagttcacca gattgcccga
    33781 cttggcggtg tcgacggtgg tgactggaga accttttgcc acaccagctg ggcaggtgat
    33841 ggcaccgcgt ccgctgccgc cgcaggcggt ggcactgtca tattcagtat gataattatc
    33901 cagacggatc ccgccgttca gctcaaaatc acgggtgatt tgcagcgtat cgaaggcgta
    33961 aattgcgaag gtatccgtct gaccattggc gtttgcgccg ttgcgcgtca ggccgccggg
    34021 atgaattctg ctgtcaggat gataaatatt taccgccggt aacgtcaccg gattaacgcc
    34081 atagttagtc tgcgtttcac gggtaaattc cacgccggta ctgacatcat gaccgataga
    34141 accggtatag aacgtcgagg tcaggttggt ctggttggtg agaattttat tactcacatc
    34201 tttggtattc gccgtgcgtg actaggtcca gctattcaca tcgctggtgg gctgagtaat
    34261 attcgacgcc ccgcccataa tcgccgtcat caggtaatcc tgctttacgc gcgaccaacg
    34321 ggtagtattg cgaatggtgg tgttatcgtt gatgtcgtgc tcaaaacgca tggtggcggt
    34381 gtcggtggtc gaatcgtcgt aatcggaatc cgtgccgtaa aagttatgag tatcaacttt
    34441 tccggaatga ttcagggccg ccgctcccgc agatggggca gaatagcccg gcaaaccgat
    34501 ggtcggaatg ccgccgtctg gcgtgttgtg ctgagtgaca tgcagataat taagatacaa
    34561 acgatttgct gtaccaaggc caaaagcgac agaaggggcg acgccgtaac gctcattttt
    34621 gactttgtcg cgtccggcat cgtgggtttt ttcgcccatc acattcaggc gcaccgcggt
    34681 agtatcacca atgacctgat tgacgtccag cgtgccgcgg cggaaccagg cgctgccaat
    34741 actggcggag gcgtcaatgc cggaatcatt gcgcggctgc ttgctgatca tattgatcga
    34801 gcctgtcggt gcgctgcgcc cgtagtcggt gccggacggc cctttaatca cttcgacctg
    34861 ctcggtattg aaggtgtcgc gcgagacgct gccgatatcg cgaataccat caatataaat
    34921 actgttagag gtatcggcac cacgcatata aatggcgtcg ccagtggtgg agttaccgtt
    34981 ctcacccgca aaaaacgcac ccacgc
//
LOCUS       DAJTSA010000032        34906 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000032 DAJTSA010000000
VERSION     DAJTSA010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.032"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            858..3143
                     /gene="nrdA"
                     /locus_tag="OO369_001730"
     CDS             858..3143
                     /gene="nrdA"
                     /locus_tag="OO369_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR5982041.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3377..4507
                     /gene="nrdB"
                     /locus_tag="OO369_001731"
     CDS             3377..4507
                     /gene="nrdB"
                     /locus_tag="OO369_001731"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR5982042.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4507..4761
                     /gene="yfaE"
                     /locus_tag="OO369_001732"
     CDS             4507..4761
                     /gene="yfaE"
                     /locus_tag="OO369_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR5982043.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4815..5465)
                     /gene="inaA"
                     /locus_tag="OO369_001733"
     CDS             complement(4815..5465)
                     /gene="inaA"
                     /locus_tag="OO369_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR5982044.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5644..5886
                     /locus_tag="OO369_001734"
                     /pseudo
     CDS             5644..5886
                     /locus_tag="OO369_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5928..7004)
                     /gene="glpQ"
                     /locus_tag="OO369_001735"
     CDS             complement(5928..7004)
                     /gene="glpQ"
                     /locus_tag="OO369_001735"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5982045.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(7009..8367)
                     /gene="glpT"
                     /locus_tag="OO369_001736"
     CDS             complement(7009..8367)
                     /gene="glpT"
                     /locus_tag="OO369_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR5982046.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8640..10268
                     /gene="glpA"
                     /locus_tag="OO369_001737"
     CDS             8640..10268
                     /gene="glpA"
                     /locus_tag="OO369_001737"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR5982047.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            10258..11517
                     /gene="glpB"
                     /locus_tag="OO369_001738"
     CDS             10258..11517
                     /gene="glpB"
                     /locus_tag="OO369_001738"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR5982048.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11514..12704
                     /gene="glpC"
                     /locus_tag="OO369_001739"
     CDS             11514..12704
                     /gene="glpC"
                     /locus_tag="OO369_001739"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR5982049.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12897..13847
                     /locus_tag="OO369_001740"
     CDS             12897..13847
                     /locus_tag="OO369_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR5982050.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13888..14358)
                     /locus_tag="OO369_001741"
     CDS             complement(13888..14358)
                     /locus_tag="OO369_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR5982051.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14413..15615)
                     /locus_tag="OO369_001742"
     CDS             complement(14413..15615)
                     /locus_tag="OO369_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR5982052.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15702..16244)
                     /locus_tag="OO369_001743"
     CDS             complement(15702..16244)
                     /locus_tag="OO369_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5982053.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16523..16948
                     /gene="nudI"
                     /locus_tag="OO369_001744"
     CDS             16523..16948
                     /gene="nudI"
                     /locus_tag="OO369_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR5982054.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16987..17589)
                     /locus_tag="OO369_001745"
     CDS             complement(16987..17589)
                     /locus_tag="OO369_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR5982055.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17897..19036
                     /gene="arnB"
                     /locus_tag="OO369_001746"
     CDS             17897..19036
                     /gene="arnB"
                     /locus_tag="OO369_001746"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR5982056.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            19040..20011
                     /gene="arnC"
                     /locus_tag="OO369_001747"
     CDS             19040..20011
                     /gene="arnC"
                     /locus_tag="OO369_001747"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR5982057.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVVSILINRNYGQHSAIMAGFSHVTGDLIITLDA
                     DLQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDY
                     GCMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRL
                     INLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFA
                     VLFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            20011..21992
                     /gene="arnA"
                     /locus_tag="OO369_001748"
                     /pseudo
     CDS             20011..21992
                     /gene="arnA"
                     /locus_tag="OO369_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            21989..22879
                     /locus_tag="OO369_001749"
     CDS             21989..22879
                     /locus_tag="OO369_001749"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR5982058.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22879..24531
                     /gene="arnT"
                     /locus_tag="OO369_001750"
     CDS             22879..24531
                     /gene="arnT"
                     /locus_tag="OO369_001750"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR5982059.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24528..24863
                     /gene="arnE"
                     /locus_tag="OO369_001751"
     CDS             24528..24863
                     /gene="arnE"
                     /locus_tag="OO369_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR5982060.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24863..25249
                     /gene="arnF"
                     /locus_tag="OO369_001752"
     CDS             24863..25249
                     /gene="arnF"
                     /locus_tag="OO369_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR5982061.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25243..25515)
                     /gene="pmrD"
                     /locus_tag="OO369_001753"
     CDS             complement(25243..25515)
                     /gene="pmrD"
                     /locus_tag="OO369_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR5982062.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25620..26975)
                     /gene="menE"
                     /locus_tag="OO369_001754"
     CDS             complement(25620..26975)
                     /gene="menE"
                     /locus_tag="OO369_001754"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR5982063.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26972..27934)
                     /gene="menC"
                     /locus_tag="OO369_001755"
     CDS             complement(26972..27934)
                     /gene="menC"
                     /locus_tag="OO369_001755"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR5982064.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27934..28791)
                     /gene="menB"
                     /locus_tag="OO369_001756"
     CDS             complement(27934..28791)
                     /gene="menB"
                     /locus_tag="OO369_001756"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR5982065.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28806..29564)
                     /gene="menH"
                     /locus_tag="OO369_001757"
     CDS             complement(28806..29564)
                     /gene="menH"
                     /locus_tag="OO369_001757"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR5982066.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29561..31231)
                     /gene="menD"
                     /locus_tag="OO369_001758"
     CDS             complement(29561..31231)
                     /gene="menD"
                     /locus_tag="OO369_001758"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR5982067.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31320..32615)
                     /gene="menF"
                     /locus_tag="OO369_001759"
     CDS             complement(31320..32615)
                     /gene="menF"
                     /locus_tag="OO369_001759"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR5982068.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32694..32999)
                     /gene="elaB"
                     /locus_tag="OO369_001760"
     CDS             complement(32694..32999)
                     /gene="elaB"
                     /locus_tag="OO369_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR5982069.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(33054..33515)
                     /locus_tag="OO369_001761"
     CDS             complement(33054..33515)
                     /locus_tag="OO369_001761"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5982070.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33580..34497
                     /gene="rbn"
                     /locus_tag="OO369_001762"
     CDS             33580..34497
                     /gene="rbn"
                     /locus_tag="OO369_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR5982071.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34688..34876
                     /locus_tag="OO369_001763"
                     /pseudo
     CDS             34688..34876
                     /locus_tag="OO369_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         8536 a         9342 c         8649 g         8379 t
ORIGIN      
        1 cgtaatgcct gaaaggcttt gcctgctggc gtgacaactc gctttgatat catatccctg
       61 gcataaatca atggccgccg cgacaccgtt agcagagaaa agagatgcaa tcattgacgg
      121 gagtagagat aaatactcct tgcgtagaaa gataatccgc atattaatct gaccatcatt
      181 ttatataaga cggcatattt aaacgcatta tacaatatgg gttatctttc ttttgtaaat
      241 tgtccaaccc gatgcaatgg ctattttgtt attatttgat gaataatatc agtgcgtcat
      301 aattcaagtt aataacctta agggatacca gttatattta aactaaatta aagtaatgaa
      361 taactttctt ataatataag gtaaattaac aaaatggctt cgcatttaac aataaccgaa
      421 tagaaaacaa ccatttcgac atcaacaatc gcttacattc gcttatatat tgaccacaac
      481 tgatacatca gattatgtga tgactcgtgc ttagatcaat ttttgcaatc attagcaaaa
      541 agattaataa accatctata tcaatttatc caacctattc tgccgttcaa gaaatcgccg
      601 aacagttatt tttaacaaat ttttctcttc ccattgactt tcccggacac cttgtctgac
      661 ctaaggtgcg cgaaagccac tttttccttc ctgagttatc cacaaagtta tgcacttgca
      721 agagggtcat tttcacacta tcttgcagtg aatcccaaac atacccccta tatatagtgt
      781 tctaagcagc ttcccttact acaggtagtc tgcatgaaac tattgcggaa agaattccaa
      841 aaacaggtac gacatacatg aatcagaatc tgctggtgac aaagcgcgac ggtagcacag
      901 agcgcatcaa tctcgacaaa atccatcgcg ttctggattg ggcggcagaa ggactgcata
      961 acgtttcgat ttcccaggtc gagctgcgct cccacattca gttttatgac ggtatcaaga
     1021 cctctgacat ccacgaaact attatcaagg ctgccgcaga cctgatctcc cgtgatgcgc
     1081 cggattatca gtatctcgcc gcgcgcctgg cgatcttcca cctgcgtaaa aaagcctacg
     1141 gccagtttga gccgcctgcg ctgtacgacc acgtggtgaa aatggtcgag atgggcaaat
     1201 acgataatca tctgctggaa gactacacgg aagaagagtt caagcagatg gacacgttta
     1261 tcgatcacga ccgtgatatg accttctctt atgctgccgt taagcagctg gaaggcaaat
     1321 atctagtaca gaaccgcgtg accggcgaaa tctacgaaag cgcccagttc ctttatattc
     1381 ttgttgccgc gtgcctgttc tcgaactacc cgcgtgaaac gcgcctgcaa tacgtgaagc
     1441 gtttttatga cgcggtttcc acatttaaaa tttcgctgcc gacgccaatc atgtccggcg
     1501 tgcgtacccc gactcgtcag ttcagctcct gcgtactgat cgagtgcggt gacagcctgg
     1561 attccatcaa cgccacctcc agcgcgattg tgaaatatgt ttcccagcgt gccgggatcg
     1621 gcatcaacgc cggacgtatt cgtgcgctgg gcagcccgat tcgcggtggc gaagcgttcc
     1681 ataccggctg tatcccgttc tacaaacatt tccagacagc ggtgaaatcc tgctctcagg
     1741 gcggtgtgcg cggcggtgcg gcaacgctgt tctacccgat gtggcatctg gaagtggaaa
     1801 gcctgctggt gttgaaaaac aaccgtgggg ttgaaggcaa ccgcgtgcgt catatggact
     1861 acggggtaca aatcaacaaa ctgatgtata cccgcctgct gaaaggtgaa gatatcaccc
     1921 tgttcagccc gtccgacgta ccggggctgt acgacgcgtt cttcgccgat caggaagagt
     1981 ttgaacgcct gtataccaaa tatgagaaag acgacagcat ccgcaagcag cgtgtgaaag
     2041 ccgttgagct gttctcgctg atgatgcagg aacgtgcgtc taccggtcgt atctatattc
     2101 agaacgttga ccactgtaat acccatagtc cgtttgatcc gaccatcgcg ccagttcgcc
     2161 agtctaacct gtgcctagaa attgccctgc cgaccaaacc gctgaacgac gtcaacgacg
     2221 agaacggtga aatcgcgctg tgtacgctgt ctgctttcaa cctgggcgca attaatagcc
     2281 tggatgaact ggaagagctg gcgattctgg cggttcgtgc acttgacgcg ctactggatt
     2341 atcaggatta cccgatcccg gccgccaaac gtggagcgat ggggcgtcgt acgctgggta
     2401 ttggtgtgat taacttcgct tactatctgg cgaagcacgg taaacgctac tccgacggca
     2461 gcgccaacaa cctgacgcat aaaaccttcg aagcgattca gtattacctg ctgaaagcgt
     2521 ctaatgagct ggctaaagag caaggcgcgt gcccgtggtt taacgaaacc acttacgcga
     2581 aagggatcct gccgatcgat acctataaga aagatctgga tgccatcgct aatgagccgc
     2641 tgcattacga ctgggaagca ttgcgtgaat caatcaaaac gcacggcctg cgtaactcca
     2701 cgctttctgc cctgatgccg tccgagacct cttcgcagat ctctaacgcc actaacggta
     2761 ttgaaccgcc gcgcggttac gtcagcatca aagcgtcgaa agacggtatt ttgcgccagg
     2821 tggtgccgga ctacgagcac ctgcacgacg cctatgagct gctgtgggaa atgccgggta
     2881 acgatggcta cctgcaactg gtaggtatca tgcagaaatt tatcgatcag tcgatctctg
     2941 ctaacaccaa ctacgatccg tcacgtttcc cgtcaggaaa agtgccgatg cagcagttgc
     3001 tgaaagacct gctcaccgcc tacaaattcg gggtcaaaac gctgtattat cagaacaccc
     3061 gtgacggcgc tgaagacgcg caggacgatc tggtaccgtc aatccaggac gatggctgcg
     3121 aaagcggcgc atgtaagatc tgatattgag atgccggatg cggcgtaaac gccttatccg
     3181 gcctacggct cggtttgtag gcctgataag acgcgccagc gtcgcatcag gctccgggtg
     3241 ccggatgcag cgtgaacgcc ttatccggcc tacggctcgg atttgtaggc ctgataagac
     3301 gcgccagcgt cgcatcaggc acaggatgcg gcgtaaaatg ccttatccgg cattaaactc
     3361 ccaacaggac acactcatgg catataccac cttttcacag acgaaaaatg atcagctcaa
     3421 agaaccgatg ttctttggtc agccggtcaa cgtggctcgc tacgatcagc aaaaatatga
     3481 catcttcgaa aagctgatcg aaaagcagct ctctttcttc tggcgtccgg aagaagttga
     3541 cgtctcccgc gaccgtatag attaccaggc gctgccggag cacgaaaaac acatctttat
     3601 cagcaacctg aaatatcaga cgctgctgga ttccattcag ggtcgtagcc cgaacgtggc
     3661 gctattgccg cttatttcta ttccggaact ggaaacctgg gtcgaaacct gggcgttctc
     3721 agaaacgatt cattcccgtt cctatactca tatcattcgt aatatcgtta acgatccgtc
     3781 tgttgtgttt gacgatatcg tcaccaacga gcagatccag aaacgtgcgg aagggatctc
     3841 cagctattac gatgaactga tcgaaatgac cagctactgg caccttctgg gcgaaggcac
     3901 ccacaccgtt aacggtaaaa ccgtaaccgt tagcctgcgc gagctgaaga aaaaactgta
     3961 tctctgcctg atgagcgtta acgcgctgga agcgatccgc ttctacgtca gctttgcttg
     4021 ttcctttgcc tttgcagaac gcgaactgat ggaaggtaac gccaagatta ttcgcctgat
     4081 tgcccgcgat gaagccctgc acctgaccgg tactcagcat atgctgaatc tgctgcgcag
     4141 cggcgcggat gatccggaga tggcggaaat tgccgaagaa tgtaagcagg agtgctatga
     4201 cctgtttgtc caggcggcac agcaagagaa agactgggcg gattatctgt tccgtgacgg
     4261 ttcgatgatt ggtctgaata aagatattct ctgccagtac gttgaataca tcaccaatat
     4321 ccgcatgcag gcagtcggtt tggatctgcc gttccagacg cgctccaacc cgatcccgtg
     4381 gatcaacacc tggctggtgt ctgataacgt gcaggttgct ccgcaggaag tggaagtcag
     4441 ttcttatctg gtcgggcaga ttgacgcgga agtggacacc gacgatttga gtaacttcca
     4501 gctctgatgg cccgcgttac cctgcgcatc actggcacac aactgctgtg ccaggatgaa
     4561 cacccttccc ttctggcggc gctggaatcc cacaatgtgg cggttgagta ccagtgccgc
     4621 gaaggttact gcggctcctg tcgcacccga ctggtcgcag gtcaggtgga ctggattgcc
     4681 gaaccgttag cctttattca gccgggggaa attttgccct gttgttgccg ggcaaaaggc
     4741 gatattgaaa tcgagttgtg atcgggtact agcgccagat acgacgctta cgcgtattat
     4801 ctggcctaca acgattacat ggcgtagtaa tacgctttca cctgctccca gtccgcttta
     4861 gggatcggct ccagatattt ttccagctgg cggaagtcat gattaatcgc tttatcgcga
     4921 cgcaagcggc gacgactttt ctccagatca agaaaaccag cttctgcctt accttctgtt
     4981 ttcacataga tatggcgaac atagcaacag ccatgctgac gattaacgct atgcattttc
     5041 ttaaacgcca gtgccactgc tttcaacatg gcttgccgta cttcgtcaga ataaggcgat
     5101 actgcatgcc gggcatacca gtcagcaatg ctgatgaacc ccgccatatc ttcagtcacc
     5161 agcaacgcgc gccattcacc ctcaattttc accgcttcac caaaaacgat cttcggcacg
     5221 atgacaccgg cccgttccag ttctttaatt accgcaacct cacggacaat cgttggtcgg
     5281 ccgaacggat aacgtactga atgaaacaga tgatgcgtca tgcgctttac atacagcttt
     5341 ttgccgttgc gctcgacgca ttgcaccccg ctcataccat tacggcgata gttaggctct
     5401 tcaacccagt cgccctctgt tgcccaccag tgattaaatt cgtcgtactt tgctgaaact
     5461 gccataccct atcgcctgtc atttttatta acgacaatga ctataggtgg ttacctgagg
     5521 aaaatcttaa tgaaacgtgt cgtattaatg aatttctttt gccgctacag gtgtgacacc
     5581 gcttttgccc ttcatttgca gcgcatagat tccaccagcc actaccacta atagtgcgcc
     5641 cagcagataa aatgtgaatt taatcagtcg gatacagcga tgaattttat tcgacaggga
     5701 ttaggcatcg ccttacaacc agagttaacg ctgaaaagca ttgcaggtga attgtgttcc
     5761 gttcctcacg aaccaacctt ctatcgacag atttcgttgc tgactaaaga aaagccggta
     5821 gaaggcagtc cactgttttt actacaaatg tgcatggaac aattagtggc gattggaaaa
     5881 atttgatatg agacaggatg gcgcatgaac gccatcctgc agtaacatta ctctttatta
     5941 agaaatttta ctgccttatc agggaaatca gtaaacagcc catttacacc cgctttgtta
     6001 tacagagcat catataactg attcacatca ggagtgtatt caggcagttt atctgaccgc
     6061 acggtataag gatgcactac cagtttattc tgctgggcat cttgcaccat gccagtgagt
     6121 ttgatattac ccggctgcga tgtctcctca atcaacatat ggtaatccgg accaatacca
     6181 tctgcatatt ccgccacctg tttcatggct cccggcttaa acatccagtc gtagttgtaa
     6241 ttaacccagc ttccatccgg ctgtttctgc tgcgtttcat tccagtcggt ataggcaatc
     6301 agctgtacca gattgagctc catgcccatt ttgggttcca gctcattctt aatacgcttc
     6361 agctcatcag catcaaaaca ttgcaaataa actttatcgt ctttaccggt gtaaccatat
     6421 ttcttcagca cttccagcgt ttttgccgca atatccttcc cttcctgatg atggaaccac
     6481 ggcgctttga tttctggata gataccgata tttttcccgg tagagtgatt taacccctga
     6541 acaaattcaa tctcttcttc aaaggtgtgc acccggaagt cggacttacc cattgggaaa
     6601 cgccccggat aagtctgtac ttttttaccg ttttcaatat cgaaaccttc ggtaaatttc
     6661 agcgacttaa tttcatccag cgtgaaatct atcgcgtagt aacgaccgtc tttgcgcgcc
     6721 cgatccggga aacgatcggc aacatcagta acacgatcga gataatggtc atgcagaaca
     6781 accagatggt cgtctttggt catcaccaaa tcctgttcca gataatccgc tccctgcgca
     6841 tacgccatcg cttttgctgg cagcgtatgc tccggcaaat atccactggc accgcgatgg
     6901 gcgatgacta ttttttcgtt gctgtccgcc gccattgcac tgcttcccat gactatagtg
     6961 ctcatcatga tcgccatgct aaggtttttc agcttcaatt tcatgccatt agcctccgtt
     7021 gcgtttttgc agtaattgtt catggcgacg tttttcgcca atcatcacaa caatcaacaa
     7081 gataaccgcc agaatgctgc cgccaatcat taccataaag ccgccatccc agccgaagaa
     7141 gtccacggtg tagccaacaa tcgcgctcgc cgccaccgaa ccgcccaggt aaccaaacag
     7201 cccggtaaag cccgctgccg tacctgccgc ttttttcggt gccagttcca gcgcatgcag
     7261 accgatcagc atcacaggac cgtagatcag gaagccgata acaatcatac aaatcatatc
     7321 gacggttggg ttacctgccg ggttcatcca gtaaacgata gtcgcgatgg tcaccagtgt
     7381 cataaagaag acgccggttg ccccacggtt accacggaag actttgtccg acatccagcc
     7441 gcacagcagg gtgcccggaa tacccgcata ctcatagaag aagtaggccc aggaggattt
     7501 atccagcgcg aaatgcttaa cctctttcag ataagtcggt gaccagtcga ggatgccgta
     7561 acgcagcaga taaacgaaca cgttggcgat ggcgatgtac cacagcagtt tgttcggcag
     7621 cacgtactgc atgaagattt gcttcgcggt cagctcctgt tccgcttttt cgttatagtc
     7681 gtccggataa tcatttttgt actcttcgat cggcggcaag ccacaggatt gcggggtatc
     7741 gcgcatcatc gcaaaggcga ataatgccac cagaatggcg cagaaagcag gcatatagag
     7801 cgccgcatgc cagtcattga accaggccat ccccagcagg aacagcagcg gcggaatacc
     7861 accaccgacg ttgtgcgcac agttccacac tgacacaatg ccgccacgtt ctttctgcga
     7921 ccaccagtgc accatagtac gaccacacgg cggccacccc atcccctgga accaaccgca
     7981 gaggaacaac agtacaaaca tcaccgcaat gctcgacgtc gcccatggca caaagcccat
     8041 aaacaacatc actgccgccg ccagaatcaa acctgcgggc aggaaaacgc gcggattcga
     8101 gcgatccgat accgaaccca tgatgaattt cgaaaatcca taagcaatcg agatccccga
     8161 aagggcaaaa cctaaatcac cgcgtgagaa tccctgctca accagataag gcatagcaag
     8221 cgcaaagttc ttacgaacca aatagtaagc cgcatagcca aagaatatcc ccaggaaaat
     8281 ttgccagcgc aatcgacgat aagtcggatc gatctccgcg gcaggtaagc gcgctttgtg
     8341 tggcgctggt ttaaaaatac tcaacattga tagcctccgt ggcccgtggt cttatttatg
     8401 attaacagcc tgattcagtg agagaacctg ccgtttcttg agttgccgcg atgttaagaa
     8461 aacattcata aattaaatgt gaattgccgc acacattatt aaataagatt tacaaaatgt
     8521 tcaaaatgac gcatgaaatc acgttttact ttcgaatcat gagcgaatat gcgcgaaatc
     8581 aaacaattca tgtttttact atggctaaat ggtaaaaaac gaacttcaga gggataacaa
     8641 tgaaaactcg cgactcgcaa tcaagtgacg tgattatcat tggcggcggc gcaacgggag
     8701 ccgggattgc ccgcgactgt gccctgcgcg ggctgcgcgt gattttggtt gagcgccacg
     8761 acatcgcaac cggtgccacc gggcgtaacc acggcctgct gcacagcggt gcgcgctatg
     8821 cagtaaccga tgcggaatcg gcccgcgaat gcattagtga aaaccagatc ctgaaacgca
     8881 ttgcacgtca ctgcgttgaa ccaaccaacg gcctgtttat caccctgccg gaagatgacc
     8941 tctcattcca ggccactttt attcgcgcct gcgaagaagc agggatcagc gcagaagcta
     9001 tagacccgca gcaagcgcgc attatcgaac ctgccgttaa cccggcactg attggcgcgg
     9061 tgaaagttcc ggatggcacc gttgatccat ttcgtctgac cgcagcaaac atgctggatg
     9121 ccaaagaaca cggtgccgtt atccttaccg ctcatgaagt cacggggctg attcgtgaag
     9181 gcgcgacggt gtgcggtgtt cgtgtacgta accatctcac cggcgaaact caggcccttc
     9241 atgcacctgt cgtggttaat gccgctggga tctgggggca acacattgcc gaatatgccg
     9301 atctgcgcat tcgcatgttc ccggcgaaag gatcgctgct gatcatggat caccgcatta
     9361 accagcatgt gatcaaccgc tgccgtaaac cttccgacgc cgatattctg gtgcctggcg
     9421 ataccatttc gctgattggt accacctctt tacgtattga ttacaacgag attgacgata
     9481 atcgagtgac ggcagaagag gttgatattc tgctgcgtga aggggaaaaa ctggcccccg
     9541 tgatggcgaa aacgcgcatt ttgcgggcct attctggcgt gcgcccgctg gttgccagcg
     9601 atgacgaccc gagcggacgt aacgtcagcc gtggcatcgt gctgctcgac catgctgaac
     9661 gcgatggtct ggacggattt atcaccatca ccggtggcaa actgatgacc tatcggctga
     9721 tggctgaatg ggctaccgac gcggtatgcc gcaaactggg caacacgcgc ccctgtacga
     9781 ctgccgatct ggcactgcct ggttcacaag aacccgctga agttaccttg cgtaaagtca
     9841 tctccctgcc tgccccgctg cgcggttctg cggtttatcg tcatggcgat cgcacgcctg
     9901 cctggctgag cgaaggccgt ctacaccgta gcctggtatg tgagtgcgaa gcggtaactg
     9961 cgggtgaagt gcagtacgcg gtagaaaatt taaacgttaa tagcctgctg gatttacgcc
    10021 gtcgtacccg tgtggggatg ggcacctgcc agggcgaact ctgcgcctgc cgcgctgccg
    10081 gactgctgca acggtttaac gtcacgacgt ccgcgcaatc tatcgagcaa ctttccacct
    10141 tccttaacga acgctggaaa ggcgtgcaac ccatcgcctg gggagacgca ctgcgcgaaa
    10201 gcgaatttac ccgctgggtt tatcagggac tgtgtggtct ggagaaggag cagaaagatg
    10261 cgctttgata ctgtcattat gggcggcggc ctcgccggat tactctgtgg cctgcaactg
    10321 caaaaacacg gcctgcgctg tgccattgtc acgcgtggtc aaagcgcgtt gcatttctca
    10381 tccggttcgc tggatttgct gagccatctg ccagatggtc aacccgtggc agacattcac
    10441 agtggactgg aatctttgcg tcagcaggca cctgcacatc cttactctct tctcgggcca
    10501 caacgcgtgc tcgatctcgc ttgccaggcg caggcattaa tcgctgaaag cggtgcgcaa
    10561 ttgcagggca gcgtagaact ggctcaccag cggattacgc cgctcggcac tttgcgtgca
    10621 acctggttaa gttcgccaga agttcccgtc tggccgctac ccgcgaagaa gatatgtgta
    10681 gtggggataa gcgggctgat ggatttccag gcgcatcttg cggcggcttc gttgcgcgaa
    10741 ctggatcttt cggttgaaac cgcagaaata gagctgccgg aactggatgt cctgcgcaat
    10801 aacgccaccg aatttcgcgc ggtgaatatc gcccgtttcc ttgataatga agaaaactgg
    10861 ccgctgttac ttgatgcgct tattcctgtc gccaatacct gcgaaatgat cctgatgccc
    10921 gcctgcttcg gcctggccga tgacaaactg tggcgttggt tgaatgaaaa actaccttgt
    10981 tcactgatgc ttttgccaac gctgccgcct tccgtgctgg gcattcgtct gcaaaaccag
    11041 ttacagcgcc agtttgtgcg ccaaggtgga gtgtggatgc cgggcgatga agtgaaaaaa
    11101 gtgacctgta aaaatggcgt agtgaatgaa atctggaccc gcaatcacgc cgatattccg
    11161 ctacgtccac gtttcgcggt tctcgccagc ggcagtttct ttagtggcgg actagtagcg
    11221 gaacgtaacg gcattcgcga gccgattctc ggccttgatg tgctacaaac cgccacgcgg
    11281 ggtgaatggt ataagggcga tttttttgcg ccgcaaccgt ggcagcagtt cggtgtaacc
    11341 actgatgaga cgctacgccc gtcgcaggca gggcaaacca ttgagaacct gtttgccatc
    11401 ggttcggtgc tgggcggatt tgatcccatc gcccagggat gcggcggcgg tgtttgtgcc
    11461 gtcagtgctt tacatgccgc tcaacagatt gcccaacgcg caggaggcca acaatgaacg
    11521 acaccagctt cgaaaactgc attaagtgca ccgtctgcac caccgcctgc ccggtgagcc
    11581 gggtgaatcc cggttatcca gggccaaaac aagccgggcc ggatggcgag cgtctgcgtt
    11641 tgaaagatgg cgcactgtat gacgaggcgc tgaaatattg catcaactgc aaacgttgtg
    11701 aagtcgcctg cccgtccgat gtgaagattg gcgatattat ccagcgcgcg cgggcgaaat
    11761 atgacaccac gcgcccgtcg ctgcgtaatt ttgtgttgag tcataccgac ctgatgggta
    11821 gcgtttccac gccgttcgca ccaatcgtca acaccgctac ctcgctgaaa ccggtgcggc
    11881 agctgcttga tgcggcgtta aaaatcgatc atcgtcgcac gctaccgaaa tactccttcg
    11941 gcacgttccg tcgctggtat cgcagcatag cggctcagca agcacaatat aaagaccagg
    12001 tcgctttctt tcacggctgc ttcgttaact acaaccatcc gcagttaggt aaagatttaa
    12061 ttaaagtgct caacgcaatg ggtaccggtg tacaactgct cagcaaagaa aaatgctgcg
    12121 gcgtaccgct aatcgccaac ggctttaccg ataaagcacg caaacaggca attacgaatg
    12181 tagagtcgat ccgcgaagct gtgggagtaa aaggcattcc ggtgattgcc acctcctcaa
    12241 cctgtacatt tgccctgcgc gacgaatacc cggaagtgct gaatgtcgac aacaaaggct
    12301 tgcgcgatca tatcgaactg gcaacccgct ggctgtggcg caagctggac gaaggcaaaa
    12361 cgttaccgct gaaaccgctg ccgctgaaag tggtttatca cactccgtgc catatggaaa
    12421 aaatgggctg gacgctctac accctggagc tgttgcgtaa catcccgggg cttgagttaa
    12481 cggtgctgga ttcccagtgc tgcggtattg cgggtactta cggtttcaaa aaagagaact
    12541 accccacctc acaagccatc ggcgcaccac tgttccgcca gatagaagaa agcggcgcag
    12601 atctggtggt caccgactgc gaaacctgta aatggcagat tgagatgtcc acaagtcttc
    12661 gctgcgaaca tccgattacg ctactggccc aggcgctggc ttaaactccc ttctgatgcc
    12721 cggtaagcat gtggttaccg ggcatttttg cgtacacgat tccgtgccca atgtatgcgt
    12781 tgcaacgcag tgaaaattcc tctgaaaacg tctcgcaaag gctgaaactg gcagatgtca
    12841 aaggcctggg ataaccgtaa tgtcgcgtca tcataaatat caggtgacgg acaaccatga
    12901 ccgaatcaac aacctcctcc ccgcatgatg cggtatttaa aacctttata ttcacacccg
    12961 aaaccgcacg ggattttctc gaaatacatt taccagaacc actgcgcaag ctttgcaacc
    13021 tgcaaacctt acgcctggaa cccactagtt ttattgaaaa aagtttacgc gcttactact
    13081 cggatgtttt gtggtccgtg gaaaccagcg acggtgacgg ctatatctac tgcgtgattg
    13141 aacatcaaag ctctgcagaa aagaatatgg cttttcggcc aatgcgctat gccactgccg
    13201 ccatgcagag tcacctagat aaaggctatg acagagttcc gctggtggtg ccattgctgt
    13261 tttatcatgg cgaaacatcg ccctacccgt actcacttaa ctggctggat gagtttgacg
    13321 atccgcaact tgcccggcag ttgtacaccg aagcttttcc gttggtggat atcaccatcg
    13381 tacctgacga tgagatcatg caacatcggc gtatagctct gctggaactg attcaaaagc
    13441 atattcgcga ccacgattta atcggcatgg tcgacaggat caccacgctt ttggttagag
    13501 gcttcactaa tgacagccag ctacaaacac tgtttaatta tctgctgcaa tgcggcgata
    13561 cctcccgttt cacccgtttt attcaggaga ttgccgaacg ttcaccacta caaaaggaga
    13621 gattaatgac tattgctgaa cggctacggc aggaaggaca tcaaattggc tggcaagaag
    13681 gtaaattagt aggtttgcag caaggtaaat tagaaggttt gcaggaaggt atgcatgaac
    13741 aagccattaa aattgctttg cgcatgctgg aacagggcat tgatcgtgac caagtgctcg
    13801 cggccaccca gctaagcgaa gccgatctgg cagcgaataa ccactaatta acacaggccc
    13861 acagccgatc cccatgggcc tttgatatca ataactacct tttatgcgtg gcccattttt
    13921 gcctgattta aacatcgcca gtcgttgatc cagggcatcg ctgtagagca tcgtgtcaac
    13981 gccaacagcg acaaagttcg ctccccacgc caggcattgc tgcgccatat caggagccac
    14041 agccagaaaa ccagccgctt tacccgcagc acggatccgc cgaatgctgg tttcaataat
    14101 tcgctgcact tccgggtgcc cggcgttatc cgggtagccc aacgacgcag aaagatccgc
    14161 tggtccaata aacacgccat caatcccttc gacgtcgagg atctcgtcca ggttatccag
    14221 tgccgtttta ctttccacct gcactaacag acaaagcgaa tcgttgactt gcgccatgta
    14281 attctcaatg cgtccccagc gcgcagcccg tgccacactg gccccaacac cacgctcacc
    14341 gtagggaggg ttggacaaat tttgccggag ggggaatgtg ccggtgcagt aaaccggcac
    14401 agagtggcga aatcagacgg ataaagtcat ggactcaaca acctcaatcc agccatgctc
    14461 actagcgatg tcctggccgt ttaaccaacg gcgtagcata ttcagtgcca tcattgcgca
    14521 cacttcctga cggatagcca ggctgtagcg tgtagtgctg aaacgcacac gcagagcgaa
    14581 agtgccgtct ggcgtggcta gcgcaaagtt gagatgctcg ttctcgaaac ccgaaacagc
    14641 cagtgccagc ccggcaaaat ggttggcccg ccgttctgta atccagtgcg cagtttgcgc
    14701 cagggtttct tcctgtgaag gaatcacttc acacgccagc aatggagcac ctgcgcgaga
    14761 aagttgcaaa gccaataaac caccggtgaa ttgctcgctc aacgtcaggc tgaactggcg
    14821 gttttgcaat tcgcgactga tctgcgcggg cagtccttcg gtgccttcga aaatcacgct
    14881 ctgtccggca acacgtttaa cgtccagcca cagtttttcc atcgcctgtt gctcgcttgc
    14941 cggtccggtg agtttcagtt cgatgatcgg cattgaggag cgatagccca ttgttacgcc
    15001 cggcggcagt tgtagagtgt ccaggctttg tgccagatcg ctttccgaac gaccaaaggt
    15061 agttaaacgc agacaaaccg gcggctgtgg taaagaaaaa cgctcgcgca ggcgcggcag
    15121 gatttcgtgc tcgaccatca ccttaaattc tgacggtacg ccgggagtaa agaacatcag
    15181 gcaacgatta agctgcacgg caaaaccaca ggcggtgcct accgggttat tgataaattc
    15241 agcactggca ggcagctccg cttgtttacg gttgctcggt gccattactc gtccacgttc
    15301 gtggaaatag cgttccatct ctttgagcca ggcttcatgc agcaccaggc cttcaccttt
    15361 tgctgtcgca gcggcgagtg cgcttaaatc atcgctggtc ggtcccagcc cgccgttaac
    15421 gatcagcaca tcggcgtgct gactacgttc gcgaagaatg gtgactaagt catcaaggtt
    15481 atcccccacc gtattgcggc gagataatgg cagcccctga tgaaagaaaa aatcggccag
    15541 ccaggcagcg ttagtgtcaa cgatttgccc gtataacact tcatccccgg tggatagcat
    15601 ttccactttt aacatggtta tctcctgctt ttgttatcga atcactataa cgcaaaggga
    15661 gataaggcgt agtaagaaac tgaatgccgc gccggaaggg atcagaaact ggcgttaacc
    15721 ccaacataca ggccgtcagc cacagcgttg tcgcggttac cgtctttacc cgacagattc
    15781 aggtagcgat aacccgcctc aatactgact ggacgcataa tggtgtaacg cgcgccagca
    15841 ttcgcttcct cataactttg aataccgctg gagagcgaat ccggagagta gtaatactcg
    15901 ccaaacaaac ggaagctgtt gccaattttc cactgcaaac cacctcctac cgctgcggca
    15961 taaccttcat cgccgtaatt cgggttggtg tacacgcctt ttccgccaac ggtcgccatt
    16021 aacggcccga gaggcagatt caaccccagc cccacgcccg cgacgtcacc gtcgtcgtcg
    16081 ttatgtgtcc agttaccgct taaagccagg cccgtcgatt cagtcccaaa accgacacca
    16141 atattggtgt attctttacc cgcctggccg ctgatgctca ttgcattgac cgatgcagaa
    16201 accaacagca ttccggccag acctgctagc gcaatttttt tcattatccc ctttcccaca
    16261 agcaattaat caaaattccc aaagctgcgg gattgtactg cctggcacag aggattcaat
    16321 gagcgaaaaa agggatctag tcacttattg taactaaata cttcttaaat aattaaacgc
    16381 cgaaaaaata aacacacttt accgatacac acaaaaataa taataagtgc agttatcatt
    16441 gcaaaagaga ttactgtcac tttcacatat ttctgagtta ccgtattctc atggtttctt
    16501 tttatttata aggaataccc ctgtgcgaca acggactatt gtatgccctt tgattcaaaa
    16561 tgatggtgct tatttgctgt gcaaaatggc cgacgatcgc ggcgttttcc ccggtcaatg
    16621 ggcgctttcg ggtggcggcg tggagtctgg cgaacgaatt gaagaggcac tacgccgcga
    16681 aattcgcgaa gaactgggag aacagctgct tttgacagaa atcacgccgt ggaccttcag
    16741 cgatgatatt cgcaccaaga catatgcaga tggtcgcaag gaagagattt atatgattta
    16801 cctgattttt gactgcgttt ctgccaaccg agaagtgaaa ataaacgaag agtttcagga
    16861 ctacgcgtgg gtaaaacctg aagatctggt gcattatgat ttaaatgttg ccacccgaaa
    16921 aacgttacgt ttgaaaggtc ttctttaaca atgatgacag ccatcactgg ctgtcagatt
    16981 gaaaatttag tggttaacga attccccatc cagataaact ttgccttttt ccacatgcat
    17041 gactaaacca tccagataat caggtttaaa tatcgcatca cgcttatttt tagcaatata
    17101 tgtcaggcaa tgattatggg tgaaaataac gatattttta tcaggcgctt tgctttgtaa
    17161 gtccttaatt gcactataaa tctcattacc gcactgaaga agtcgtttat ctaccgtcag
    17221 ctttttaccc gcggaaaacc aggtcgccga ctgaatggtc cggacggtat tactggaata
    17281 aagatcgaaa tcagagatgt cagcgctaaa agcgttgccc agttcacggg catcctgggt
    17341 acctttaacc gtaatacctg ttttatctga caagcattga ttggttgaac gatcgcaacg
    17401 ttcagcatgg cgaaacaaaa cgacaaccgg gtgctgctgt gccagtctgg ccaatgtttt
    17461 attgtcgata cgtggcaaac cattgctact ccaggcggca tgagtaccca gtccggcaat
    17521 tgcagcgagc gccagtaaaa tgatgaaata tttttttgac ttcaatgatg agcggcaaaa
    17581 agctaacacg taaactccac ctatagacaa gcgcaaccag acaattaccg tgaaattgag
    17641 cgacatttct ggcgataatt agcagtcggt gtaatattaa aaatcctacg atgtcggcaa
    17701 aatgcctcaa atttttgcca aatgcaaagc ctaaataaga aaaaatataa aaatttcaat
    17761 atttacgtct aatattagtt tcttaaggtt aagttaatat tctatcctta aaatttcgct
    17821 ccaaatggca aaatatacac aacactcttt atagcaaata taagtggaca ggtattcaat
    17881 ggcggaagga aaagcaatgt cagaattttt gcctttttcg cgaccagcaa tgggcgtgga
    17941 ggaactcgct gcagttaaag aggttctcga atccggttgg atcacaaccg gtccgaagaa
    18001 tcaggcgctt gagcaagcct tttgccagtt gacgggaaat cagcatgcca tcgcggtcag
    18061 ttcagccacc gccggaatgc atatcacgct aatggcgttg gaaattggca agggcgatga
    18121 agtgattacg ccttccctga cctgggtttc aaccctcaat atgatttcct tgctgggtgc
    18181 aacgccggta atggtggatg tcgaccgcga tacgctgatg gtcacgcctg aagcgatcga
    18241 atcagccatt acgccacgca ctaaagccat cattccggtg cattatgccg gtgcgccagc
    18301 agatattgac gccattcgcg ccattggcga acgttacggc atcgcagtta tcgaagatgc
    18361 tgcccatgcc gtcggtacgt attacaaagg gcgacatatt ggcgcaaaag gtaccgctat
    18421 tttttcattt catgccatta aaaatattac ctgtgctgaa ggtggcctga ttgtaactga
    18481 taatgaaaac cttgcccgcc agctacggat gctgaaattt cacggtctgg gtgtcgatgc
    18541 ctatgacaga caaacctggg gccgtgcacc gcaggctgaa gtcttaacac cgggctataa
    18601 gtacaatctg accgatatta acgccgcgat tgccctgaca cagttagtca aattagagca
    18661 cctcaacacc cgtcggcgcg aaattgccca gcaatatcag caagcactgg cagctctccc
    18721 ctttcagcca ttaagccttc ccgcctggcc gcacgttcac gcctggcatc tgtttattat
    18781 tcgtgtcgat gaacaacgtt gtggtatcag tcgcgatgcg ttgatggaag cgttaaaaga
    18841 aagaggcatt ggtaccgggt tacatttccg cgccgctcac acacaaaaat attatcgcga
    18901 gcgttttccc acgctgtcgt taccgaatac cgaatggaat agcgaacgca tctgttcgtt
    18961 gccgctgttc ccggatatga ctaccgccga tgccgaccgc gtcatcacag cccttcagca
    19021 actcgcagga caataagcca tgtttgaaat ccaccctgtt aagaaagtct cggtggttat
    19081 tcccgtttat aacgagcagg aaagcttacc ggaattaatc aggcgcacca ccacagcctg
    19141 tgaatcgttg gggaaagagt atgagatcct gctgattgat gacggcagta gcgataattc
    19201 cgcgcatatg ctggtcgaag cctcacaagc ggagaacagc catattgttg tgtctatttt
    19261 gattaaccgc aattacgggc aacattcagc gattatggcg ggattcagtc acgttactgg
    19321 cgacttaatt attacccttg atgccgatct ccagaatccg ccagaagaaa tcccccgcct
    19381 ggtggcaaaa gccgatgaag gttacgacgt ggtagggact gtacgccaga accgccagga
    19441 cagctggttt cgtaaaacag cttcgaagat gattaaccgg cttattcagc gcaccactgg
    19501 caaagcgatg ggtgactacg gttgtatgct gcgcgcctat cgccgtcata ttgtcgatgc
    19561 gatgttgcac tgccatgaac gcagcacctt tatcccgatt ctggcgaata tcttcgcccg
    19621 ccgtgcaatt gaaattccag tacatcatgc cgagcgtgag tttggtgaat ccaaatacag
    19681 ttttatgcgc ctgattaatt tgatgtacga cctggtgacc tgccttacca caacgccgct
    19741 acgtatgctg agtctgctcg gcagcattat tgcgattgga ggttttagca ttgcggtgct
    19801 gctggtgatt ttacgcctga ccttcggacc acaatgggcg gcagaaggcg tctttatgct
    19861 atttgccgtg ctgtttactt ttattggcgc tcagtttatc ggcatgggat tactcggtga
    19921 atatatcggc aggatctaca ccgatgtccg cgcccgcccc cgctattttg ttcagcaagt
    19981 tatccgtcca tccagcaagg aaaatgaata atgaaaaccg tcgtttttgc ctaccacgat
    20041 atgggatgcc tcggtattga agctctgctg gctgccggtt acgaaattag cgccattttt
    20101 acccataccg ataatcccgg tgaaaaagcc ttttatggtt cggtggctca tctggcggcg
    20161 gaaagagaca ttccggttta tgcgccggat aacgttaatc atccgctgtg ggtggaacgc
    20221 attgcccaac tgtcaccaga ggtgattttc tctttttatt atcgccatct tatttgcgac
    20281 gagatttttc agctcgctcc cgcaggtgca tttaatctgc acggttcgct gttaccaaaa
    20341 tatcgtggtc gcgcgccgct gaactgggtg ctggtgaacg gtgaaacgga aacgggcgtt
    20401 acattgcacc gaatggtaaa cgtgccgatg ctggggccat tgtggcgcaa ctgcgcgttg
    20461 ccattgcgcc agacgatatc gctattacgc tgcatcataa attgtgccat gccgcgcgcc
    20521 agctactgga acagacatta cccgccatta aacacggtaa tattctggaa atcgcccagc
    20581 gcgaaaacga agccacctgt tttggtcgca gaacgccgga tgacagtttc cttgaatggc
    20641 ataaaccggc atccgtactg cacaacatgg tacgtgccgt tgccgatccg tggccgggtg
    20701 ccttcagcta tgttggcaat cagaaattca ccgtctggtc gtcgcgtgtt catcctcatg
    20761 ccagcaaagc acagccgggg agcgtgattt ctgttgcgcc actgctgatt gcctgtggcg
    20821 atggcgcgct ggaaatcgtc accggacagg cgggcgacgg cattactatg cagggctcgc
    20881 aattagcgca gatgctgggc ctggtgcaag gttcacgctt gaatagccag cctgcctgca
    20941 ccgcccgacg ccgtacccgg gtactcatcc tcggggtgaa tggctttatt ggcaaccatc
    21001 tgacagaacg cctgctgcgc gaagatcatt atgaagttta cggtctggat attggcagcg
    21061 atgcgataag ccgttttctg aatcatccac attttcactt tgtcgaaggc gatatcagta
    21121 ttcattccga atggattgag tatcacgtca aaaaatgtga tgtcgtcttg ccgctggtgg
    21181 cgatagccac gccgattgaa tatacccgca acccgctgcg cgtatttgaa ctcgattttg
    21241 aagagaatct gcgcattatc cgctactgcg ttaagtaccg taagcgaatc atcttcccgt
    21301 caacttcaga agtttatggg atgtgtagcg ataaatactt cgatgaggac cattctaatt
    21361 taatcgtcgg cccggtgaat aaaccacgct ggatttattc ggtgtcaaaa caattacttg
    21421 atcgggtgat ctgggcctat ggcgaaaaag agggtttaca gttcaccctc ttcctcccgt
    21481 ttaactggat gggaccacga ctggataacc ttaatgcagc gcgaattggc agctcccgcg
    21541 ctattacgca actcattctc aatctggtag aaggttcacc aattaagcta attgatggcg
    21601 gaaaacaaaa acgctgcttt actgatatcc gggacggtat cgaggcgttg taccacatta
    21661 tcgaaaatgc gggaaatcgc tgcgacggcg aaattatcaa cattggcaat cctgagaacg
    21721 aagcgagcat tgaggaactg ggcgagatgc tgctggcgag cttcgaaaaa catccgctgc
    21781 gccatcattt cccaccgttt gcgggctttc gcgttgtcga aagtagctgc tactacggca
    21841 aaggatatca ggacgtagag catcgtaaac cgagcatccg caatgcccac cgctgcctgg
    21901 actgggagcc gaaaattgat atgcaggaaa ccatcgacga aacgctggat ttcttcctgc
    21961 gcaccgttga tcttacggat aaaccatcat gaccaaagta ggcttacgca ttgatgtcga
    22021 tgcctttcgt ggcacccgtg aaggcgtgcc gcgtctgctg gaaatcttga gtaagcataa
    22081 tattcaggcc agcatttttt tcagcgtcgg cccggacaat atgggccgcc atctctggcg
    22141 actggtgaag ccacagtttt tgtggaagat gctgcgctca aacgcggcat cgctttatgg
    22201 ctgggatatt ttactggcag gtacggcctg gccaggtaaa gagattggtc atgccaatgc
    22261 cgatatcatt cgtgaagcgg ctaaacatca cgaagtcggt ctgcacgcct gggatcacca
    22321 tgcctggcaa gcccgtagcg gtaactggga tcggcaaaca atgatcgacg atattgcacg
    22381 cggtcttcgc actctggaag agattatcgg tcaaccggta acctgttctg ccgctgcggg
    22441 ctggcgtgcc gaccagaagg tgatcgaagc aaaagaagcg ttccatttgc gctacaacag
    22501 cgattgtcgt ggggccatac cgttccgtcc actgcttgaa tcaggaaacc ctggcactgc
    22561 gcaaattccg gtgaccttac ccacctggga tgaagtgatt ggtcgggatg tgaaagcaga
    22621 agattttaac ggttggttac tcaaccgcat cctgcgagat aaaggcacgc cggtttatac
    22681 cattcatgca gaagttgaag gctgcgctta tcagcataat tttgtggatc tcctcaaacg
    22741 cgcagctcag gaaggcgtga cattttgccc tttaagcgaa ctgttatcag agacgttgcc
    22801 gctcggacaa gttgttcgcg gaaatattgc cggacgtgaa ggctggctgg gttgccaaca
    22861 aattgcgggt agtcgctgat gaaatcggta cgttacctta tcggcatctt cgcgtttatt
    22921 gcctgctatt acctgttacc gatcagcacg cgtctgctct ggcaacccga tgaaacgcgt
    22981 tatgcggaaa tcagtcgaga aatgctggca tccggcgact ggattgtgcc ccatctgtta
    23041 gggctacgtt atttcgaaaa acccattgcc ggatactgga ttaacagcat tgggcaatgg
    23101 ctatttggcg cgaataactt tggtgtgcgg gcaggcgtta tctttgcgac cctgttaact
    23161 gccgcgctgg tgacctggtt tactctgcgc ttatggcgcg ataaacgtct ggctctactc
    23221 gccacagtaa tttatctctc attgtttatt gtctatgcca tcggcactta tgccgtgctc
    23281 gatccgttta ttgcattctg gctggtggcg ggaatgtgca gcttctggct ggcaatgcag
    23341 gcacagacgt ggaaaggcaa aagcgcagga tttttactgc tgggaatcac ctgcggcatg
    23401 ggggtgatga ccaaaggttt tctcgccctt gccgtgccgg tattaagcgt gctgccatgg
    23461 gtagcgacgc aaaaacgctg gaaagatctc tttatttacg gctggctggc ggttatcagt
    23521 tgcgtactga cggttctccc ctggggactg gcgatagcgc agcgggagcc tgacttctgg
    23581 cactattttt tctgggttga gcatattcaa cgctttgcac tagatgatgc ccaacataga
    23641 gctccgttct ggtactacgt gccggtcatc attgccggta gcctgccgtg gctgggatta
    23701 ctccccggtg cactgtacac aggctggaaa aaccgcaagc attccgcaac cgtctatttg
    23761 ttgagctgga cgataatgcc gctgctgttt ttctccgtcg ctaaaggcaa attgcccacc
    23821 tatattcttt cttgctttgc acctctggca atgctgttgg cgcattacgc gttgctggca
    23881 gcaaaaaata atcctctggc gctgcggatt aatggctgga ttaacatcgc ttttggcgtc
    23941 actggcatta ttgccacatt tgtgatctcc ccctggggac caatgaacac gccggtgtgg
    24001 caaaccttcg agagctataa agtcttttgt gcctggtcga ttttttcgct atgggcattt
    24061 ttcggctggt acaccttaac aaacgtcgaa aagacctggt ctttcgccgc gctttgcccg
    24121 ctggggctgg cgttgctggt aggattttca attcctgaca gagtcatgga aggaaaacat
    24181 ccgcaatttt ttgtcgagat gacacaagaa tcacttcagc caagccgcta tattcttact
    24241 gatagcgtcg gtgttgccgc aggtctggca tggagcctgc aacgcgatga catcatcatg
    24301 tatcgccaga caggtgagtt gaaatacggc cttaattatc cggatgcgaa agggagattt
    24361 gtcagcggtg atgagttcgc aaactggctt aatcaacatc gtcaggaggg gattattaca
    24421 ctcgtgcttt cggttgaccg cgatgaagat atcaacagtc tcgccattcc gcccgcagat
    24481 gccatcgttc gtcaggagcg tctggtgctg attcagtatc gtcccaaatg atctggctaa
    24541 cattagtctt tgccagcttg cttagcgttg ccgggcagtt gtgtcagaaa caggcaacct
    24601 gctttgtggc gataaacaaa cggcgcaaac atatcgtgct gtggctggga ctggcgctgg
    24661 cttgtcttgg tcttgccatg atgctctggc tgctggtctt gcagaacgta ccggtaggca
    24721 ttgcctaccc gatgttaagt ctgaattttg tctgggtgac gctggctgca gtaaaactgt
    24781 ggcacgaacc ggtatcgccg cgtcactggt gtggggtggc gttcattatt ggtggcattg
    24841 tgatcctcgg gagtacggtg taatgtgcct gatatgggga ttattcagcg tcattattgc
    24901 ctcagttgcg caattaagcc tgggttttgc ggcaagtcat ctgccgccga tgacgcactt
    24961 atgggatttt atcgcggctc tgttagcttt tggcctggat gcccggatac tgttactcgg
    25021 gctgctgggt tatctgcttt ccgttttttg ttggtataaa acgctacata aacttgcgct
    25081 aagcaaagcc tatgccttgc tcagtatgag ttatgtgctg gtatggattg cttcaatggt
    25141 acttcccggc tgggaaggga ctttttcgct gaaagcacta ctgggagtag cctgtattat
    25201 gagcgggttg atgctgattt tcctgcccac gacaaaacaa cgttactgag ttttccctgc
    25261 cgctgtacac tgccgttccc actcatcggg gctataactg cttgccgaga ggactttcac
    25321 cgtatgcagc ttttcacgat taatacaata aagcgcattc tgcaaaggcg agagtaaatc
    25381 ccccactttc acggcgaagt cactcttcac ttcggcgatc atttttatcg cgccgccagc
    25441 atcgcagagc ataagtacat gtctgttatc ttgtttattg cagcacgatt ttttgtgcca
    25501 gccattccat tgcattatcc tgtttgctaa gagttttcac atcaatttac agattaacag
    25561 ataaactctt tatcaacggc aatggaatgg cagaaatgag aagtgaaacg gagggttaat
    25621 tattgctgac gttgcaccca ctcttttagc gcctgacgtg aaattttaat accgccgttt
    25681 ttcagctccg gcggtaaagt cagccagcgc accggttgtt gaaaacgagc cagcttatct
    25741 ttcacccatt cgccaagatc aacggtttgc tggtcatact ccaccaccgc taccggccga
    25801 tgaccaaact ccttgtcggc aacggaaacg ataaacacct gcaaaaccgc aggatgtgca
    25861 gcaataacac gctcgacttc ttccggctga ataccctcac cgccactgaa gaacagattg
    25921 tctaaacgtc cgacaatggt cagcttgcca ttatgcatct caccgcgatc gcgcgtagcg
    25981 taccagcctt cgtcattaac cagtgaaacc agttgcccgt tacgccagta accttctgcc
    26041 atactggcag cccgcagcca cacttcatta ttaacgattt tcacttcccg acccggcagc
    26101 ggcaaaccaa cgtctgccag gccgtcggct tctttcgcac ataccgtgga ggcaaactcg
    26161 gtcagaccat agccgcaaaa gcaacgaatc ccctgctcgc gcgcctgttc cgtcaactcg
    26221 accgggatag ccgcgccgcc aagtaacacc gctttcaggg aaacggaact gcggttaacc
    26281 agcaaacgcc agagttgtgt tggcaccagt gaagcgtgag tacagcctgc cagcatttgc
    26341 tccaatggct gtttatcacg caccgtcatc cgcgcaccag cgtataacca gcgccacata
    26401 attccctgac cggagacgtg aaacagcggt aaagagagca accagtcatc gtgatcgcca
    26461 aacggaatca gcgataacac accttgcgca ctggcaagat gggcctgata agtatggaca
    26521 gcggctttcg gcaaaccggt agaacccgag gtcaacgtca ttgagcacag acgcgtcggc
    26581 tgccacgcag cggcatgtac gccttcaacc agctgaatgt gcagcgacgt taatgccgga
    26641 aacgtgtttt ccccatccgg caccagcgca aattgcagcg tcagattggg cagcaattct
    26701 tcaagcaacg gttgcggcag ctgagggttc acgggcaaca cccgcgcccc gcattgcagt
    26761 aacgccagcc aggcgagcag cgtttgcggc gtattccacg cccgcaacat cacgccgctg
    26821 ccctcaacca ccccctgcgc cgcaaagccc gtagctaact catcgacgcg agcacaaagc
    26881 tcgcgccagt tgagttgctc gtcattaaga cgtaaggcga tggcttctcc ccgcacttgc
    26941 cgccagtgac gccacggcca gtcagagaag atcataacaa ctgctcctgt acatcagcgt
    27001 ccactaacgg cagtgggctc cccggccagc gacgaacctg ctgcgcctgc atcagatcca
    27061 gcgtgtccag tcccggaatg gtgtctggcg ttaaccaggc agcaatccgc gccagttgcg
    27121 ttagacctaa gctcgactca atggaagaac tgatcaccgc cgtcagcccc agcgcatgcg
    27181 ccgcctgtac ctgctcgcgt actttttcca gactgcccgt gagcgtgggt ttgataacta
    27241 ccgcgcgcac gccctcttca gccacaaagg caaaatccgg ctcgcgcagg ctttcatccc
    27301 aggcaatggc aatgccggtt tcacgggcaa acgctcgcga atcatcgcgg gttttgcacg
    27361 gctcttcgag aaacgcgatg cggtggcgat aatccgggtt aacatatttg gcaaactgct
    27421 gacctttcag cggtgtccag gcgcgatttg cgtcaagacg caaatgcaga tccggaattg
    27481 cctccagcaa cagattcacc accatgccgt cgcgcaccgc ttcgtacaat cccactttga
    27541 ccttcgccac tttctcgccc ggcatatcgg caagtttgag gatcagatcg tccggatcgc
    27601 cattacacag cggtgccgca cggtagttgg ctgcttgcgg caacgtatct gtcagttctg
    27661 ccaatgcaca gcttacgcca aaggccacgg aaggcatctg cggtagctcg caatcgcctg
    27721 ccagccagtt atttacccag gcaagcagca cgctttgcgc ctcttcccag ttttcctggc
    27781 tgaagcccgg cagtggggag atctcccccc acccttcgcg ctcgccctca cgcaggcaaa
    27841 cgtacagccc gtcgcgggtt tttaaccgcc tgtcgcgcag aaccaccccc gcgtccatgg
    27901 ggatctgcca gcggtatacc tgcgcgctac gcattacgga ttccgtttaa atttgctgaa
    27961 atcaggctga cgtttctggt tgaaagcgtt gcgaccttcc tgaccttctt ccgtcatgta
    28021 gaacagcatg gtggcgttgc ccgccagctc ctgcagcccc gcctgcccgt cacagtcggc
    28081 gttcagcgca gctttcaggc agcgcagcgc catcgggctg ttttgcagca tttcgcggca
    28141 ccaacggacg gtttcttttt ccagatccgc cagcggtacc acggtgttga caaggcccat
    28201 atccagcgcc tgttttgcgt cgtactgacg gcacaggaac cagatttctc gcgctttttt
    28261 ctgcccgacg atacgagcca tgtaggaagc gccccagccg ccgtcgaagg aaccgacttt
    28321 cgggccagtc tgaccgaaga tggcattatc tgctgcgata gtcaggtcgc acatcatgtg
    28381 cagaacgtga ccaccgccga tggagtagcc agccaccatc gcgacaaccg gtttcggaca
    28441 ggtacggatc tgacgctgga agtccagcac gttcaggtga tgtacgccgg aatcatcttt
    28501 atagccgccg taatcaccac gcactttctg gtcaccaccg gagcagaacg ctttatcgcc
    28561 tgcgccagtc agaatgatca cgccgatatt gtcgtcataa cgcgcatctg ccagtgcctg
    28621 gatcatctct tttaccgtca gaggacggaa ggcattgcgc acctgcggac gattaatggt
    28681 gatttttgcg ataccgtcgg tggatttttc ataacgaatg tcctcgaaac cttcggagca
    28741 gtcgtgccat tcaaccggtg cgtaaggcat tgcttcatca ggataaatca tattgtgtcc
    28801 tttagtcaga aacgcaagat ctgcgccaga cttgcgatta cgccagcggg attttcccga
    28861 tgcgcgttat gtccggcgcg aggaatgaca tggcagtcgg cagccagttc cgccgccagg
    28921 gcgcggaatt tgctgtcacg ttcaccacat aaataataaa acgcaaatgt gcgggcgcta
    28981 aggttagcac gtaaatcagg ctggacggcg agagaagtcg cctccagcat ggcggcaagg
    29041 gttgcgccat tattgttgct gcgcagcgcc accagctccc ggcgttgatc gtcattgagc
    29101 gaggcaaaaa caggctgttg ataccagtcg gcaaataccg ccgttaacgg ttctgtgcga
    29161 aaacgctgcg cccattggcg atcggaacgc tgacgttccg cacgttgttc agcattttgc
    29221 agccccggat gcccgccttc gacaataacc ccacaaagcc ccgccagccc ctggcaagcc
    29281 gccatcatcg ccacccgtcc gccaagcgag taccccacca gccagaagtc aaggatgttg
    29341 taactaacca aggttttacg cagtaagtcg gtgacatcat caaatccatc gacgctaatc
    29401 gccgccgaac caccatgacc taggagatca acatacaacc gtgagtagtc ggcaaacgcc
    29461 tcgcccactt cttgccattc gtggcaatcg ccggaaaaac cgtgcaaaaa caccagccag
    29521 ggtaaaccta gttttccgtg ttttgcctgc gcgtgcagga tcataaatgg cttacctgcg
    29581 ccagaagttg ctggagcgtt tgcgcaccat cggtgtcgtt aaccaccatt tcaatcaccg
    29641 tggtggttgg cgtgcgccag gcgtcggcaa atgccgtttc aagttcctgc cagttttgcg
    29701 gatgatgata tttcagctcg aacatcgcgg cggcgtgctc aaaatggacg ttttgcggca
    29761 tcagatagaa acgctcacgc tcgctttgcg gcgttggcaa cagcgagaaa atttgcccgc
    29821 cgttgttgtt caccacaatt aataccagcg gcgcagaaac ctgacgcaat aacgccagcg
    29881 cgttgagatc gtaaagcgcg gagagatcgc ccacaatcgc cagcgtcggt ttgccgcttg
    29941 cccgctgaac gcctgcggcg gtcgaaagca gtccgtcgat accgctggcc cctcggttgc
    30001 tgtacaccgg gtaacctgcc ggaagttgcg aaagcgcatc aatcagacgt accaccaggc
    30061 tgttaccaac aaacaattgc ccctgttcag gcagatagtc gctgatgcga tgcgccagtt
    30121 gcgcttcgcc aaacgcatca cggcgggcaa taaccgcctg cattgcctgt tccgccaggc
    30181 gcgggatttc aacgcaccag ggctggcgtt tttctgccgg atgcagctcc agccagtcgg
    30241 caatattggc aattaagcga cgtccgcgat ggtgtgccgg atcaagtcgc ccttcaatgt
    30301 catcaacaat ccagtactct tctggttcac agcttgcctg ccattgcagg agccgtttcc
    30361 ccgtcaggct acttcccagt tgcaccacaa tttgcgcctg ctgcagctcg ctggtcgctt
    30421 tggcattgcc taaccaaaga tcggcacacg gcagcggctg cccggtttgt gacagcacat
    30481 cgccaatcag cggccagcca agagtttgcg cccacagggc aacttttttg ccctcttccg
    30541 cactcatgcg cccggcaacc accacgccgc gcttttgtcg ccagaagaac cagtcgcgct
    30601 gtttttcact ttccagacga ggcgcttcac gcagccacgg tttgtcgtcc tgccaccagt
    30661 cacccagacg ctgttgccag ctaagcccgg tatcgtccat ttcgccatac agcggttcag
    30721 caaacgggca gttgatatgg actccccccg catgaagcgt accgagagcg tggtcgatgg
    30781 tagaaaccag ccaacgtgcg gggatatcct gggtcgggcg cggcaatgaa atactgtgcg
    30841 tggggtgaga ggcgaacatt cccggctggc gaattgcctg attcgcgccg cagtcaatta
    30901 gctccggcgg gcgatcggcg gttaagagaa tcagtttttc tccggttaac ccggcttcaa
    30961 tcagtgccgg atagagattt gccaccgccg tgccggaggt cacaatcacc gccaccggct
    31021 gcttgctgac tttcgccagc cccagcgcca gatgccccaa cccacgctca tcgaaatggg
    31081 tgtggtgaat gaatgcggaa ttctccgccg ccgctaacgt taacggtgta gaacgcgagc
    31141 ctggggcgat acagatgtgt ctgacgccgt gacgcgttaa tgcttccaga atgaccgccg
    31201 cccagcgtcg gttaaatgcg cttactgaca tgagtttgtc cggtatcaat attgcggcta
    31261 agtataagga gctacaaaaa tagattattg atatgaatcg gtaatgatgc gactcattac
    31321 tattccattt gtaataaagt acgcagccct gccgctttgt tgtcgatttc ctgccactct
    31381 tgctcggggt cggaaccacg gacaatgccc gcgccagcat ataatcgcac gacattgccg
    31441 ctaatttttg ctgagcgcag ggaaacgcag aattcgcttt gttgtaatga gagatagccc
    31501 gcagaaccgg cgtaccattc tcgggtgaac ggttcgtgac gggcgataaa ctgtcgcgcc
    31561 agatcgcgcg gtaagccagc aactgctgcc gttggctgca actgatgtaa acagatcaca
    31621 tcatccgctt tgttgagtga agtccagata cagcggcgaa gatgctgcac tttacgcaga
    31681 cgcagtacct gcggcggtaa aacatccagc gtctgggtat cggcctgtaa tcgttggcag
    31741 atatcttcca ccaccagcat gttctcgcgc tggtttttat catccgccat cagccactct
    31801 cctaactgct gcgcctgctt atcatcagga tgatttgcta ctgttcccgc cagcgcttca
    31861 gtacgcagcg ctttgtcacg ccgccgccat aaccgttccg gtgaagagcc aagaaaagca
    31921 ttttcgccat caaaggccat gtaaaaatgg tagcaattca gattcagtcg acgactggca
    31981 gccatcatcg ccgccgcgtt gaccggactt gcgaaatgca ggtcagttgc ccgagcgagc
    32041 accactttgt cgagctcacc ttcggcgatg gttttcgttg ccagttcgat taattgcgtc
    32101 cagcccgttt tgtccggcca gtgttgttct cgcgtggtgg ttaaatgtaa cccaggcaag
    32161 ggcttgatac tcaccagtgt ggcgataaat tcttttgcct gaatcgcatc gtgctgaagg
    32221 gagctttcgc tgaatagcgt cagccgcagc gtggctttac cgccacagcg tcgccattcc
    32281 aggcggggta aaagtaaatt gccctgcgac gggtcaaaag cattcagccc ccaaatgcgt
    32341 aagtcagcgt gttccgggtg ctggcgaaga aaacgttgtg cctggtccaa cgacgtaaaa
    32401 cgggtaatcg cgcccaggac ggcagcttct tcatcaccat tacgttgttg ccagtagaat
    32461 tgcgggtacg tttgctgact ggccagccag ctcaaggcat caaaagcgtc tttgagaggg
    32521 aaaggaatat cgataacccg aatgccgggt gtcgccggaa tctcttgcga caaatggcgc
    32581 agtagatttt ccagcgccgt agtaagtgat tgcacgcgga cctctccctg ttaaaagcct
    32641 cacattatac ggggtactac aaaaaaaatg cagtaccccg gtgtagggag gttttaacgg
    32701 cgtgccagca acagtcctag taccagcccg acggccgcgc ccacaccaat tccttgccag
    32761 ggtttttcgt ggacgtagtc atcagcacga taaacagcct gcttcgcccg atagtaataa
    32821 ctgtctgaag cctggctaac ccgttttttc acatcatcca gcgctttttc tgcatgcgct
    32881 ttcagctcaa catatttctg atcggcggga tcgccagagg agcggagcac ctcttccagt
    32941 gtttcactaa gcagcgtcag gtcgtcatcg atacgtgtat caccaaactg attagacatt
    33001 ctcgttctcc atttgcgtaa aacctgtcac taactataga caagagttgt tgattacgcc
    33061 tgaattacct cgcgtgccat cccaatgtgt gggataccat cttcttcata gacctccgtc
    33121 acagggataa aaccgaaact ctggtagaag ttttgcagat gcgcctgcgc cccgaggtaa
    33181 acaggcttat caggccagtg atgcgtacat gtttcgagtg ttttactcat cagctgctgc
    33241 cccacttttt caccacgcaa cgcttcactg acaatcaccc gacctataac gaccggctca
    33301 agatcatcat cacttttcag aatcctcgca tacgccacca gttcatcatt tttccaaccg
    33361 agaatatggc gattatcccc cgtcaaatca tcaccatcga tatcctgata agggcaattc
    33421 tgctcgacaa caaacaccgc gcaacgcagt tgtaataagg cataaagctg ggaaaccgat
    33481 aattcagagt gatgcagatc ttgccattca atcataatgc tctcctttta gatgtatgcc
    33541 ttatactagc cggaatattt tttgaaacgt gatgaactca tggaattaat ttttttaggt
    33601 acttcagccg gtgtgccaac ccgcacgcgc aatgtcacgg caatattgct taacctgcaa
    33661 catccgaccc agagcggact ttggttgttt gactgcggtg aaggcaccca gcatcagcta
    33721 ctgcataccg cctttaaccc tggaaaactg gacaagattt ttatcagtca ccttcatggc
    33781 gatcatcttt ttggtttacc cggcttgctg tgcagtcgtt ctatgtcagg cattatccaa
    33841 cccttaacga tttatggtcc gcaaggtatc cgtgaatttg tggaaaccgc gctgcggatt
    33901 agcggctcat ggaccgatta tccgctggaa attgtcgaaa ttggcgctgg cgaaattctc
    33961 gatgatggcc tgcgcaaagt aaccgcttat ccgctggaac acccactgga atgttatggc
    34021 tatcgtattg aagaacatga taaaccgggt gcattaaatg cccaggcatt aaaagctgct
    34081 ggcgtgccgc ctggcccact gtttcaggaa ttaaaagcgg gcaaaacaat cacgctggaa
    34141 gatggaaggc agattaacgg cgcagattac ttagctgctc cagtgccagg taaagcgctc
    34201 gctattttcg gcgataccgg cccctgcgat gccgcacttg acctggctaa aggtgtcgat
    34261 gtcatggtgc acgaagcgac gctggatata accatggaag ccaaagccaa tagtcgcggc
    34321 catagctcta cacgccaggc tgcgacacta gcccgtgagg ctggagtcgg caagctaatc
    34381 attacccacg tcagctcgcg ctatgatgac aaaggttgtc aacacctgtt acgtgaatgc
    34441 aggtcaattt tcccggcgac tgaactggcg aatgatttca ccgtgtttaa cgtttaactc
    34501 tgcttattat taaaaacagg gtgaaacttg ccctgttata gcaacccgcg ctacatatat
    34561 ccacccacac aggcatattg agcgattttt gatatttatt catctcctta cgcactatat
    34621 tcagagcaat aagttgcctc ttttcatctt gattcagcac aacctttgca actaaaagga
    34681 gcccatgatg gttacagttg tcagcaatta ttgtcaatta tctcaaaccc aactcagtca
    34741 gacatttgca gaaaaattta ctgtgaccga ggaattactg cagtctttaa aaaaaacagc
    34801 gttatccgga gatgaagaaa gtattgagtt actgcataat attgcgttag gttatgatga
    34861 atttggaaaa aaagctgggt aatgctgcca acttactgat ttagtg
//
LOCUS       DAJTSA010000033        34788 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000033 DAJTSA010000000
VERSION     DAJTSA010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.033"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..2098
                     /locus_tag="OO369_001764"
     CDS             <1..2098
                     /locus_tag="OO369_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR5982072.1"
                     /translation="FNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNA
                     QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLG
                     SFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQA
                     GKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNL
                     AISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYES
                     ISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSA
                     SRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTD
                     YRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYA
                     TVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDE
                     ESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAG
                     NRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLE
                     INGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQ
                     PKLRGNVQTAQREVAP"
     gene            2095..3183
                     /locus_tag="OO369_001765"
     CDS             2095..3183
                     /locus_tag="OO369_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR5982073.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="OO369_001766"
     CDS             complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="OO369_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR5982074.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5054..6466
                     /gene="uxaC"
                     /locus_tag="OO369_001767"
     CDS             5054..6466
                     /gene="uxaC"
                     /locus_tag="OO369_001767"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR5982075.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6481..7968
                     /gene="uxaA"
                     /locus_tag="OO369_001768"
     CDS             6481..7968
                     /gene="uxaA"
                     /locus_tag="OO369_001768"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR5982076.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8051..8602
                     /locus_tag="OO369_001769"
     CDS             8051..8602
                     /locus_tag="OO369_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR5982077.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="OO369_001770"
     CDS             complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="OO369_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR5982078.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10447..11412)
                     /gene="alx"
                     /locus_tag="OO369_001771"
     CDS             complement(10447..11412)
                     /gene="alx"
                     /locus_tag="OO369_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR5982079.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11683..12681)
                     /locus_tag="OO369_001772"
     CDS             complement(11683..12681)
                     /locus_tag="OO369_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5982080.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12760..13452)
                     /locus_tag="OO369_001773"
     CDS             complement(12760..13452)
                     /locus_tag="OO369_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR5982081.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13529..14032)
                     /locus_tag="OO369_001774"
     CDS             complement(13529..14032)
                     /locus_tag="OO369_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR5982082.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14117..15253
                     /gene="rlmG"
                     /locus_tag="OO369_001775"
     CDS             14117..15253
                     /gene="rlmG"
                     /locus_tag="OO369_001775"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR5982083.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15538..15852
                     /gene="higB"
                     /locus_tag="OO369_001776"
     CDS             15538..15852
                     /gene="higB"
                     /locus_tag="OO369_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR5982084.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15849..16265
                     /gene="higA"
                     /locus_tag="OO369_001777"
     CDS             15849..16265
                     /gene="higA"
                     /locus_tag="OO369_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR5982085.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="OO369_001778"
     CDS             complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="OO369_001778"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR5982086.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="OO369_001779"
     CDS             complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="OO369_001779"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR5982087.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20922..21341)
                     /locus_tag="OO369_001780"
     CDS             complement(20922..21341)
                     /locus_tag="OO369_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5982088.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21319..21672)
                     /locus_tag="OO369_001781"
     CDS             complement(21319..21672)
                     /locus_tag="OO369_001781"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5982089.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21673..21993)
                     /locus_tag="OO369_001782"
     CDS             complement(21673..21993)
                     /locus_tag="OO369_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5982090.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22127..23560)
                     /locus_tag="OO369_001783"
     CDS             complement(22127..23560)
                     /locus_tag="OO369_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5982091.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23623..24072)
                     /locus_tag="OO369_001784"
     CDS             complement(23623..24072)
                     /locus_tag="OO369_001784"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR5982092.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="OO369_001785"
     CDS             complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="OO369_001785"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR5982093.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="OO369_001786"
     CDS             complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="OO369_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR5982094.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28539..28870
                     /locus_tag="OO369_001787"
                     /pseudo
     CDS             28539..28870
                     /locus_tag="OO369_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="OO369_001788"
     CDS             complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="OO369_001788"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR5982095.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30709..32229
                     /gene="aer"
                     /locus_tag="OO369_001789"
                     /pseudo
     CDS             30709..32229
                     /gene="aer"
                     /locus_tag="OO369_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32383..33006)
                     /gene="nfeR"
                     /locus_tag="OO369_001790"
                     /gene_synonym="yqjI"
     CDS             complement(32383..33006)
                     /gene="nfeR"
                     /locus_tag="OO369_001790"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR5982096.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33283..34028
                     /gene="nfeF"
                     /locus_tag="OO369_001791"
                     /pseudo
     CDS             33283..34028
                     /gene="nfeF"
                     /locus_tag="OO369_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(34082..34157)
                     /locus_tag="OO369_001792"
     tRNA            complement(34082..34157)
                     /locus_tag="OO369_001792"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34122..34124),aa:Ile,seq:cat)
     gene            34282..34788
                     /gene="mug"
                     /locus_tag="OO369_001793"
                     /pseudo
     CDS             34282..34788
                     /gene="mug"
                     /locus_tag="OO369_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8483 a         8946 c         9142 g         8217 t
ORIGIN      
        1 tttcaacggt tatgatgcag atgatgtcag tgacagtaat actgacgtca gcgatctcta
       61 ttatcgttat gattttttac gtcgttatta tgtgcaggcg ggtcgcatgg acaaccgcac
      121 actatttaat gcacaaggcg ggaactttac ctttaacttt ctgccactcg gtgcaatcga
      181 tgggatgcgt atcgggtcga cactgagcta tttaaaccag gcgcaaagcc agcaaggaac
      241 cccagtaatg gttctgcttt cgcgcaattc tcgtgttgac gcttatcgta atgagcaact
      301 tttgggatcg ttttatctca atagtggttc gcaatttatt gataccagtt cctttccgcc
      361 aggcagctac agcgtggcat taaaagtcta cgaaaataac caactcaccc gcaccgagct
      421 tgtgccgttt accaaaaccg gcggtctgac tgacggaaat gcgcaatggt tcttacaggc
      481 aggtaaaact acatcacagg tttctgatga tgaaagttca gcttatcagc taggagtacg
      541 cctgccatta catccgcaat atgagctcta cgcagggctg gcgaatgctg atgatgtgag
      601 tgctttcgag ttaggtaata actggacggc agatttaggc agggtgggga atcttgcaat
      661 cagcgccagc gtgttccgta acgatgacgg cggcaaaggt gatatgcaac aggccaactg
      721 gagtaatccg ggatggccga cgttgggctt ttatcggacc aactctgacg gcgatgcttg
      781 tacaaccgac agcagagaga gctataacgc cttaagctgt tatgaaagta tttccgcgac
      841 agtttcactg aattttgtcg gctggaatat gatgctgggt tatacctgca cacaaaataa
      901 cactgatgat agtttgcgtt gggataaaca gcagagcttt gaaaataact atcttcgcca
      961 gacaacagcg caaagtatct ctgaaactgt acaacttagc gcttcccgcg ctattgtgat
     1021 gcgtgactgg attttgagta cgtcagtcgg tgttttccat cgtaatgaca acggtggcga
     1081 taacgacgac aacggcttgt acttatcgtt ttcgttatct gacacgccaa cgatggatag
     1141 caataacaac agccattcaa ccaatgtgcc tacggattat cgttatagcg aacaggatgg
     1201 cgatcaaacg tcatggcagt tatcgcatac cttttataac gattcattca gccataaaga
     1261 acttggcgta accgtcggag gcctgaacac cgataccata aacagcgcgg ttaacgggcg
     1321 ttgggatggg caatacggaa atgtctacgc taccgtatct gatagttatg accgtaagaa
     1381 tcatgatcat ctttcggcct ttacgggcac ttatagctct acgctggctg tcagtcgcta
     1441 tggcgttaat ttgggagcca gtggtacaga cgatttgctg ggtgcggtac tggtggatgt
     1501 gaaaggcttc tctgaacagg atgaagagag tcaggatctg caactcgaag cgcgggtggc
     1561 aggcagccga actttgcagc ttggtcaaag tgacagtgtg ttgttccctt atcctggatt
     1621 tcagtctggt tttgttgagg ttaacgacag tagccagggc aatcagcagg ggacaacaaa
     1681 catcattaac ggtgcaggga atcgtgaatt aatgttgttg cctggcaagc tgcgctatcg
     1741 cgaagtgtct gccagcttta actacaacta tatcggtcgt ttattactgc cggcagcggt
     1801 gaaaaaattc ccgattgtgg ggctgaacag cgccatgtta ctggtagctg aagatggcgg
     1861 atttacactt gaaattaacg gcagcgaaaa agagctctat ctgctttccg ggcagcaatt
     1921 ccttaagtgt ccactgagtg ttgtaaagaa acgcgccagc attcgttaca gcggagatgt
     1981 tacttgtagt gtggtgactt attcacaatt accggagtcg attcaggttc aggcacagtt
     2041 gaaacagcct aaattacgtg gaaacgttca gacggcgcaa agggaggttg caccatgaga
     2101 aatcgattga ttgcggcgat attgggcttg tttggcacgc tcactggcgt tcaggcagct
     2161 cctgacgtga ccagtgaaat tacgtatgat ttggcatctg gtagagcgga ttattacttc
     2221 tggaaggatg aagcgtcggc aggaaataat ggatatatgt ggtatgaatg ttcgtatcct
     2281 gacctccaac aaacctgtac agctaatgga aatatatcga cagtacaaat ctatttaact
     2341 gaacaacgca gtgggatgcg ttggccggta aaactcaaag gatttaaaac agccattgta
     2401 agtagcgatg aagcgccgct aggatgcaag gggggcaaag ggcttcagac gaatcttaag
     2461 gattctaata gatcttcatg tacagaagat ggtcaacatt attatatata cgatacaaag
     2521 tttcttacgc tctaccttga gcagacagag atgaagaatt tgccgattgg tggcgtctgg
     2581 aaggggaaag ttaaattaca ttcgaacagc ccggcccagg actatttcgc aaatattacc
     2641 ctgaatacgc tcgaccccaa ccatattgac gtgttcttcc cggagttcgc ccacgccacg
     2701 ccaagggtgc agttagactt gcatccaaca ggaagcgtta acggcagcaa ctacgcgcaa
     2761 gatctgacca tgttggacat gtgcctgtac gatggtttta acggtaatgc catcagttat
     2821 gaaatcatgc tcaaagatga agggcgacca gctgcagggc gcagagacgg ttacttctct
     2881 atctatcgtc agggagggac caccaccgac gagggagaac gcattgatta ccgggtcaaa
     2941 atgtacaacc cggaaaccgg tgggcaaatt gatgtgcgca ataatgaaaa tatggtctgg
     3001 aacagcatta acctgaaacg tgtgcgtccg gtggtactgc ccggtattcg ctatgccgtg
     3061 atgtgtgtgc cgacaccctt gactctggca gtcgataaat tcagcgtgat ggataaacag
     3121 gccgggtact acatgggcaa attgtcagta atctttacgc cttccttgcc aaccatcaat
     3181 taaccctgca aaccggacaa acgctttttt ctatataaac gacgaagccg cctgataagg
     3241 cggcttcgtc gttataactt acgttaaacg ttaatgttgc ggtgcgggat cgttatgtgt
     3301 ttcctgcgcc acctcaatcg ccggtttgtt ctgcaatacg gtccagataa ccagcgcacc
     3361 cagcaggtca aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag
     3421 tgcaccgaca accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc
     3481 gttcgccgtc gccacttcgt tacgaccgaa cacgtcagaa gagagcgtaa tcagcgcgcc
     3541 agacagtgcc tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt
     3601 ggtgaacaga ccgatcatac ccgggccaat catcagcaca gcacccagcg ttacgaccat
     3661 cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc
     3721 accgaggata caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc
     3781 tttcaggtta aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc
     3841 ccaggccggt tctgcaagga aacgcggcag tgcgataccc cagaactgac ggttacgcag
     3901 gatctgacca acggacattt tcttcgccgt gctcacctgg tgctgggctt cctgaccatt
     3961 aataatatag tcgcgttctt catcggtcag atgcttctgg tcgcgcggat gtttatagaa
     4021 aatcagccat gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg
     4081 ccagctgtgc atcacgattg cccataccac cagcggcggc gcaatcatcg caccaatcga
     4141 agaacctacg ttaaagtagc ctactgcgat ggaacgctct ttcgccggga accattcgga
     4201 gctggctttc agacccgccg ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc
     4261 aacagctagg ccaccccagc tacctgccag cgcggttgca ccacagaaca cggcccacag
     4321 cacagcaaac attgcataac cgattttcgt acccagcaca tccagtacat aacctgctac
     4381 cggttgcatg accgtataag cagcagaata ggctgcgatg atataggaat actgttgggt
     4441 ggagatgttt aactcttcca tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag
     4501 gtaaccaagc acggtgccga gcgtcaccag tgcgatcata taccaacgta accctttaat
     4561 tttacgcatg taaaacctca tcgttttgtt atatccgcag gaagcgcggc catcatcccg
     4621 ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa aaaaaaaccg gcacgccccg
     4681 aacgttgcca tgaaagtttc gtgctaattc ggcttccgta ccggttaacc cgattcatct
     4741 aacaaatgaa tgaatcggca atttgcattc cgtcgactta tgatttgcga cggcagaaag
     4801 ataacttgtc atacaacctt aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg
     4861 gacattacct gacgacagcg aggccagtac tggcgcggcc tgcagcgaga tttaccactt
     4921 tgagagtaat ttttttaact acgtttattg atctaactca cgaaaatatc ttcggactct
     4981 ggaaattggt gtgataactt tgtcagcatc gcaccataag caagctagct cactcgttga
     5041 gaggaagacg aaaatgactc cgtttatgac tgaagatttc ctgttagata ccgaatttgc
     5101 ccgccgtctg tatcacgact acgcaaaaga ccagccgatt ttcgattacc attgccattt
     5161 gccgccgcag cagattgcgg aagactatcg ttttaaaaac ctgtatgaca tctggttgaa
     5221 gggcgatcac tacaaatggc gcgctatgcg taccaacggt gtggctgagc gtctgtgtac
     5281 tggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg gcgactgttc cgcacactat
     5341 cggcaacccg ttataccact ggacgcacct cgaactgcgt cgtccgttcg gtatcactgg
     5401 caaattgctt tctccgtcaa ctgccgatga aatctggaac gaatgtaacg aactgctggc
     5461 gcaggataac ttctccgcgc gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac
     5521 caccgatgac ccgatcgatt ctctggagca tcacgcagag atcgccaaag acggctcttt
     5581 caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc ttcaatatcg aacaggcgac
     5641 ctttaacgac tacatggcga agctgggcga agtttccgat accgacattc gccgctttgc
     5701 tgacctgcaa actgccttga ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt
     5761 gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg aacgaagcgg aactggacag
     5821 cattctggcg cgccgtctgg ctggcgaaac cctgagcgag cacgaagtgg cacagttcaa
     5881 aactgcggta ctggtgttcc ttggtgccga atatgcacgt cgcggctggg tacagcagta
     5941 ccacattggc gcgctgcgta ataacaacct gcgtcagttc aaactgctgg ggccggatgt
     6001 aggctttgac tccatcaacg accgtccgat ggcggaagag ttgtctaagc tgctgagcaa
     6061 gcagaacgaa gaaaacctgc tgccgaaaac cattctctac tgcctgaacc cgcgcgataa
     6121 cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca
     6181 gttcggttcc ggctggtggt ttaacgatca gaaagacggt atggaacgtc agatgaccca
     6241 actggcgcag ctcggtctgc tgagccgctt tgtcggtatg ctgactgaca gccgtagctt
     6301 cctgtcatac acccgtcacg aatacttccg ccgcattctg tgccagatga tcggtcgctg
     6361 ggtggaagcg ggcgaagcac cggcggacat caacctgctg ggcgagatgg tgaaaaatat
     6421 ttgctttaac aatgcgcgtg actacttcgc cattgaactg aactaaggtc tggggttgat
     6481 atgcaataca tcaagatcca tgcgctggat aacgtcgcgg tcgctttagc agatttggct
     6541 gaaggcacag aagtcagtgt cgataaccag actgttacgc tgcgccagga tgttgctcgt
     6601 ggacataaat ttgcgttaac ggatatcgca aaaggggcca acgtcatcaa atatggcctg
     6661 ccgattggtt atgcattggc ggatattgcg gcgggggaac acgtccacgc ccacaatacg
     6721 cgcacgaatc tgagcgatct ggatcagtat cgctatcaac ttgattttca ggatctgcct
     6781 gcgcaagcgg cagatcctga agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg
     6841 cgtaacgagc tgtggatcct gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc
     6901 cagaaccgtt tcctgaaaga gaccaacaac gccgaaggta ccgacggcgt gttcctcttc
     6961 agccacacct atggctgctc acaactgggc gacgatcaca tcaacacccg caccatgctg
     7021 caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa
     7081 aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat
     7141 ttcatgatct gccaacagca ggatgatgag atcgaagccg ggatcgagca tttgcatcag
     7201 ctgtataacg tgatgcgcaa cgataaacgc gagccaggca aactcagcga actgaagttt
     7261 ggtctggagt gcggtggttc tgacggtctt tctggtatta ctgctaaccc gatgctgggg
     7321 cgtttctctg actacgtgat tgctaacggc ggtactaccg tactgaccga agtgccggag
     7381 atgtttggcg cagagcagtt gctgatggac cattgccgcg acgaagcaac gtttgaaaaa
     7441 ctggtcacca tggtcaacga cttcaaacag tactttattg cccatgatca gccgatctac
     7501 gaaaacccat caccggggaa caaagcgggc ggtatcacca cgctggaaga caaatcactt
     7561 ggctgtaccc agaaagcggg ttccagtgtc gtggttgacg tgctgcgtta cggcgagcgt
     7621 ctgaaaacgc cagggctgaa cttgttaagt gcgccgggta acgatgccgt agcgaccagc
     7681 gccctggcgg gtgcgggctg ccatatggtg ctgttcagta ctggtcgcgg cacgccatac
     7741 ggtggatttg tgccgacggt gaaaatcgcc accaacagtg aactggcggc gaagaaaaaa
     7801 cactggatcg actttgacgc gggtcagctg atccacggta aagcgatgcc gcagttgctg
     7861 gaagaattta tcgacaccat cgttgagttt gccaacggta agcaaacctg caacgagcgt
     7921 aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag
     7981 ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt ttgcttttcc atacctgcta
     8041 aggatttatc atgaccgcgt attggctggc ccagggcgtg ggtgtcatcg cctttctgat
     8101 tggtatcaca acatttttca atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta
     8161 tagcgccgtt attggcgtac atttttttct tctgggcacc tatcccgctg gtgccagtgc
     8221 catccttaat gccattcgta cattgattac cttacgcacg cgcagcttat gggtaatggc
     8281 gatttttatt gtgctgactg gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact
     8341 attgccggtt atcggtacga ttgtcagtac ctgggcgctg ttccgctgta aagggctcac
     8401 catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg attcacaact tctgggcggg
     8461 gtcgataggc ggcacgatga ttgagggtag ttttctgctt atgaatggcc tgaatatcat
     8521 tcgtttctgg cggatgcaga aaaggggaat tgatccgttt aaagtagaga aaacccctcc
     8581 cgccatagac gaaaggggtt aaacaattaa ttacgcaggg cgctatttgc cagacgatcg
     8641 tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt
     8701 tcgcaagagt cctgcaatac gccgataata aagccgacgg caaccacctg catggcgata
     8761 tcgttcgaaa taccgaacat attacaggcc agtgggatca gcagcaaaga accccccgcc
     8821 acgccggatg cgccacaggc acacagagaa gccactacgc tcaacagcag cgctgtgggc
     8881 agatcgaccg gaatacccag cgtattaacc gcagccagcg tcaacacggt aatggtgatt
     8941 gctgcgcccg ccatattgat ggtggctccc agcggaatag aaactgaata ggtatcgcga
     9001 tccagattca gcttttcaca cagcgccata ttcaccggaa tgttcgctgc agagctgcgg
     9061 gtgaagaagg catacacacc gctttcgcgc aggcacagca gcaccagcgg gaacgggtta
     9121 cgacgaattt tccaccacac cagcaatggg ttaaccacca gcgccaccag taacatacag
     9181 ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc
     9241 agggtagaag aaaccagccc aaaaataccg aacggtgcga agcgaatgac cagtttcacc
     9301 ataaaggtaa cggcattcga catatcattg accaggtttt tggtggtctc gttaccgtga
     9361 cgcagtgcga agccgaggcc gatcgcccac accagaatcc cgatgtagtt acctttcagc
     9421 agggcgtcga tggggttgga aaccatgctc attaccagtc cgcgcatcac ttcgacaatg
     9481 cctgacggcg gcgaaatatc acccgcgcta ctggacaagt gcagggtaga agggaaggca
     9541 aagctgaaga ctactgcggc cagagctgca gagaaggtgc ccagcagata gaggaacaaa
     9601 ataggacgga tattggtttt ctgcccgtgc tggtggttag caatagatgc cattaccagc
     9661 atcaacacca ggatgggggc aacagctttc agtgcgccga cgaacaaagt acctaacaga
     9721 ccaacagctt ccgccgcggg ttttgagatc catgccagaa gaatccccag aacaaggccg
     9781 accaggattt gttttaccag gctgccatga gccagacgcc ggaatagccc cggtgaacgt
     9841 tgcgtagtca tttttcgatc ctttcattgt gttgtcgcac atccctggtg cgttctgtcg
     9901 acgcatcttt caggatgtaa caaagtgttt gcaaggtcga gtataaggaa agttacagct
     9961 tcgggaagcg aaaaatgctg gattttaaag agaggtcatg ttttgcaact taatggttgc
    10021 atttatttaa cataaacaga gggaaggact ggctactaag gctgattaga taacatatgg
    10081 gcaaaactat ttggaatatg agaacggttg cgctccttta acgtgttatg ccatataaag
    10141 tcactggcgc agggagcgcg cagggggcgg ccaatcgccg ccgccccctg cacccccagg
    10201 ctctggcgaa caaaatcgcc gctgcgcggt tccctcagcc tatcccttgc ggctaccggg
    10261 tcgggcgcaa ggtaacgtcc ctgtaagatg cgccctcgcc ccggcctcca gggaacgctt
    10321 cggcgatttt gacgccacca acacccgtgc ggtctttttg taaagagcta taagccgatt
    10381 acataattaa tagctcacct ttgtctggaa tgagttatct ctaggaatgg cttaatatac
    10441 agcgaatcat tctccccgct gcttatcatg ccgataattc acccaggcgt tgataataaa
    10501 cgtcatcacc agaatgccaa acaccacgcc cagcgagacg gcgattggaa tatggtagaa
    10561 gtcgacaatc agcatcttga taccgataaa caccagaatc accgccaggc catatttgag
    10621 catcgagaaa cgctctgcta cgcccgccag caggaaatac atcgcacgca ggccgaggat
    10681 tgcaaacagg tttgaggtca gcacaatgaa cgggtcagtg gtcacggcga agatagccgg
    10741 aatgctatcc acggcaaaaa tcacgtcgct caattccacc agaatcagta ccagcatcag
    10801 cggtgtggcg tacagcaatc cattcttacg cacaaagaaa tgctcgttgt cgatggtgtc
    10861 ggtcatgcgc aaatgaccgc gtagccagcg caccagcggc ttgtcgccaa tgcctgattc
    10921 atcttcatgg gcgagcgcca tcttcacgcc ggtaaacagc aggaaggcac cgaagatata
    10981 caggatccag tcgaactgcg aaatcagcca gctgcctgtg aagatcatga tggtacgcag
    11041 aacaatcgcc ccgagcacgc catacaccag cacgcgacgt tgtaatgccg ccggaacgga
    11101 gaaatagctg aacaacatca gccagacaaa gacgttatcg accgccagcg atttctcaat
    11161 cagataaccc gtgagaaagg ccagggcctg tggatcggca acggcgcgac cttcggtttg
    11221 caccagatac caccagaaag cggcgttaaa cagtaacgac agcgtcaccc agaccagcga
    11281 ccaggccgca gcctgtttca tggtcatggc atgtgcccca cgacgcccct gcaacaacag
    11341 gtcgatagcc agcataatgg cgacaacaac agcgaatccg ccccatagca acggcgtgcc
    11401 gacagtattc atagaagttc cttacacata aaaaaacggc caacgtcgga agacgtcagc
    11461 cgctgctttt tatgcataga cctcgccttc cggcaaggtc tcacttacaa caaaaaagga
    11521 atacgtctgc gtattccttt cgggttgccc ggtgaccgga tgtggttttt cactcatcgt
    11581 aatgacgatc gaccggcaat gaagttactc ccctttgcag gtaacaaagt atgacagacc
    11641 attcagtcag tcaatggtct gttacatacc ttttacactg ttttactttg gcaagtatag
    11701 ttttacgcta tctgccggaa aaatcacccc agtttggcgg cggatctcgg tcagcagttt
    11761 ggctgtgatg cgactgaccg ccagccctgg atgatccacc agatgctcat ccaccagggt
    11821 agcgaacagc tctgcttcgt agagcatggt attaatatgc tgcggctggg tgagatcctg
    11881 catctggctg ccacgcggca caaagcagac tttctggcat tcagacagtt tttcaatcac
    11941 cagcgatccc gcttcgccct gaatctcgct tcccaggaca gaatcactga cctttgagtg
    12001 ctgcaaggtg acgctgaaat caccgtaatc catcaccacc acgccgtggg cgtcaacgcc
    12061 gcttgccagc aaactcgccg ttgcctgcac gcttttcggc tcaccaaata acgccaccgc
    12121 cgacgccagg cagtaaaagc cgatatccat aattgaaccg ttagagaatg ccggattaaa
    12181 ggtgttggga ttctcaccgt ccaggtaacg ttgataccgc gaggaatact ggcaatagtt
    12241 gaaaaagact ttacgcagtt tgcctgcttt cggcagcgcc tggcgcaaca aatgaaagtt
    12301 cggcaggcag gcggttttaa atgcctcaaa cagcaccacc tgattttcgc gcgcacaggc
    12361 aatggcggca tccacttccg ccagattcga cgccagcggt ttttcgcaaa tcacatgaat
    12421 tttatggcta aggaaaagtt gtgtctggga aaagtgcagg gaattcgggc tggcaatata
    12481 caccgcgtca atggcatcgc tttccgccat cgcttccagc gacgtaaaca gatgctcgac
    12541 agaaaaatca ttggcgaagt gctgagcctg ctcaaggctg cgggaatata cggcggttaa
    12601 cttgtattta ccgctctcat gggcggcctc gacgaactgg cgagtgatcc agttcgtacc
    12661 aatcacagcg aaacgtatca taacgcgtgg atactccata aagggattct gtcgtcaatg
    12721 tagcacgcct ttttcatctt cgacgtgcgc agatacgcat tattctttcg ccggacagcc
    12781 atgctcacta aacggcccaa tcatgaccga tggcccgaga tatttcggct ggcgatggag
    12841 gatttttgcg tcaatcaccg cgctgacacg agcgagtttc tcccgcagtt gagtataaaa
    12901 accgtgcttc atattgaggt ctggcgcgtt aaacccgata gcgtcgatgc cgtactgttt
    12961 tgccagccag atggtgcgtt ggttatgaaa ttcttgcgag ataatggtga tatggttttc
    13021 cccaaacacc tttttggcac gcactaccga atccagcgtt gagaatccgg catagtcgca
    13081 aaagatcact ttcgccggta cgcctttagc gatcaaagcc tgctgcatac ctgatgcttc
    13141 atcgtaattt ttacgtccgt tatcgccact caccagtagc catttcactt ttccggcgtg
    13201 gtacaacgct gctgcggtat caatacgtcg ggtaaagtag cgattacccg gcctcgcccc
    13261 cagcaataaa cccacgttac gcgccgggac ggcgttgaca tcgccccagg tcagctgctt
    13321 gctggcgttg accataacgc gatcggcgat aaaaatggtc gctcctgcca caagtaacag
    13381 taagcaggca atctttagag tccggcgaga aaaacaaata cggagaagaa ggcgggcaaa
    13441 tgcgcgcagc atggtcgtta tccttaacga aaggggggag gtcgatttta ggcgctaaag
    13501 aaagaaaaga taacgcctga catcattttc agattttgtc ctgcccaagc gacaactgcg
    13561 ttagccacag ccgggtgtcg aactcaagct ggtggtactg cggctccata tggcagcaca
    13621 attgatagaa cgctttgtta tgctctttct ccttcaggtg cgccagctcg tgcaccacga
    13681 tcatgcgcaa aaacggttcc ggcgcgttgc gaaacacggt agcgacgcgg atctctgctt
    13741 ttgctttcag cttgctgccc tgcacacgag aaacagcggt atgtaacccg agcgcgtttt
    13801 ttagcacatg gatcttattg tcatacatca ctttattgat cggcggggca ttacgcagaa
    13861 actgattttt cagatcctga gtatattgcc agagagcttt atcggtggcg tagtcgtgtg
    13921 ttcccggata gcgttttgcc agcacatcgc ccagacgctg ttcgttaatc agtgttcgca
    13981 cctgcgaaag cagctgctcg ggataaccct gaagataagt aagattgctc ataaccgccc
    14041 acgaaaaaga gaaaaagggt atactcacgc acccttttca ggggcaacgt cgaaatttta
    14101 tcattcagga gggccgatga gccacttaga caacggtttc cgttcactga cactacaacg
    14161 ttttccggcg acggatgacg ttaacccgct acaggcgtgg gaagcggcag atgaatattt
    14221 gctgcaacag ttggacgaca cagaaatcct cggcccggtg ttgatcctga atgatgcctt
    14281 tggtgcgtta agctgcgcgc tggcggaaca taagccgtac agcattggcg actcatacat
    14341 cagtgaactg gcgacgcgcg agaatttacg cctaaacggg attgatgagt cgagcgtgaa
    14401 gtttctcgac agcaccgccg actacccgca acagccgggt gtggtgctga tcaaagtgcc
    14461 gaaaacactg gcattgctgg aacagcaact gcgtgcgctg cgcaaagtgg tcacgtcgga
    14521 tacacgtatt attgccggtg ccaaagcccg tgacattcac acttccacgc tggaactgtt
    14581 cgaaaaagtg ctcggcccga ccaccaccac gctggcatgg aaaaaagcgc gcctgattaa
    14641 ttgcacgttc aatgaaccgc cgctggccga tgcgccgcag accgttagct ggaagctgga
    14701 aggtactgac tggactatcc ataaccatgc gaatgtcttc tcccgcaccg ggcttgatat
    14761 cggcgcgcgc ttctttatgc aacatctgcc agagaatctc gaaggggaga ttgtcgatct
    14821 cggttgcggt aatggcgtta ttggtctgac gctgcttgat aaaaacccgc aggcgaaagt
    14881 ggtgtttgtc gatgaatcgc cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa
    14941 catgccagag gcgttggatc gcagcgagtt tatgatcaac aacgcgctct ccggcgtgga
    15001 gcctttccgc tttaatgctg tgctctgcaa ccctccgttt caccaacaac atgcgctgac
    15061 cgataacgtc gcctgggaga tgttccacta cgcccgccgc tgcctgaaaa tcaacggcga
    15121 gctgtatatc gttgccaacc gccatctgga ttacttccat aaactgaaga aaattttcgg
    15181 caactgcacc actattgcga cgaataataa atttgtggtg ctgaaagcgg tgaagctggg
    15241 acgtcgtcgg taagattggg agttacctgc cggatgcggc gtaaacgcct tatccggcct
    15301 acgttcagca cgttaccccc aggccggata agacgcggca agcgtcgcat ccggcaatgt
    15361 ctgctcagtt ctggaatcgc gcttccataa aacaaaaaat ccctgtcacc atcctctaca
    15421 ttctcttgtt tagcgttttt ctacgtttat tcttccgtca cacagataaa ttcatccgtt
    15481 gccaatctgt caacggatgt tatcatattg ccaatttgtc aacggacgtg atgaagaatg
    15541 cacctgataa ctcaaaaagc attgaaagat gctgcggaaa aatacccgca acataaaacg
    15601 gagttggtgg ctctggggaa cacgattgct aagggatatt tcaaaaaacc tgagtcatta
    15661 aaagcagtat tcccatctct ggataacttc aaatatctgg ataagcatta tgttttcaat
    15721 gttgggggca atgaattacg tgttgtagca atggtctttt ttgaatcgca aaagtgctac
    15781 atacgtgaag ttatgacgca taaagaatac gatttcttta ccgctgttca tcgtactaag
    15841 gggaaaaaat gattgcgatt gccgacatct tgcaagcagg agaaaagcta actgctgtgg
    15901 caccttttct ggcgggtatt cagaacgagg aacaatacac ccaggcgctg gaactggtag
    15961 atcatctgct gctcaacgat cctgaaaacc ccttgctgga tctggtgtgt gccaaaataa
    16021 ccgcgtggga agaatcagcg cccgaatttg cggaatttaa tgccatggct caagccatgc
    16081 ctggcggtat agccgtgatt cgtaccctta tggatcaata tggtttaacc ctttccgatc
    16141 tgccggaaat tggcagtaaa tctatggtgt cacgcgtttt gagcgggaag aggaaattaa
    16201 cgctggaaca cgctaaaaaa ttggcaacgc gattcggcat ttctcccgcc ttgtttattg
    16261 attaacgcga cgtgccgaat gcgttgcatc cggcgcttca aataacgatt taaatctcca
    16321 gcgccagccg tgttccctgg gcaattgccc gtcgtgcgtc cagctccata gccacatcgc
    16381 agccgccaat taaatgcaca gttttcccgc tatcaatcag cggttgcgcc agcgcgcggt
    16441 ttggctcttg ccctgcgcag atcaccacat tgtccactgc taatacctgc gtttcgccgt
    16501 tgatcaccac atgcagcccg tcatcgtcaa tcttctgata acttacgcct gggatcattt
    16561 tcacaccccg cgagagcagg gtggtgcgat ggatccagcc ggtggttttg cctaacccct
    16621 gtcccggttt gctggctttg cgctggagca tcacaatttg ccgtgggcta cgcgggatct
    16681 gcattccctg cgggcttaag ccaccagcct gttgtaggct gctgtcgatc ccccactcat
    16741 tacagaacct ggcgatattc tggctggtgg attcgcccgg ctgacttaaa tacatcgccg
    16801 tatcaaaacc aatcccgcca caaccgatga tggcaacctt gttgcctacc ggcgctttgt
    16861 cgcgcagtac atcgagataa ctcaatacct tcggatgatc gatcccgtcg ataggcggaa
    16921 cacgcggcac gatcccactg gcgaggatcg tttcatcgaa cgcctgtaac tgatccgccg
    16981 tcacggtgtg attgagtttc agcgtcacgc ccgtcacttc gatcatccgg cggtagtagc
    17041 gcagcgtttc gtaaaactcc tctttgccgg ggatctgttt ggcgatatta aactgcccgc
    17101 cgatctcgct gtgcgcatca aacaatgtta cctgatgccc acgcgccgcc gcgttaatgg
    17161 caaacgccag cccagcaggt ccagcgccga ccaccgccag attttttttc tgcacggcgg
    17221 gaaggattgg cattttggtt tcgtggcagg cgcgaggatt caccaggcac gaggtgactt
    17281 tgccaacgaa gatctgatcg aggcagccct ggttgcagcc aatacaagtg ttgatctcat
    17341 cggctcgccc tgattgcgct tttgacagca gttccgcatc cgcgagaaac ggtcgcgcca
    17401 tcgatactat atcggcatcg ccgcgcgaga gaatatcgtc ggcaacctgc ggatcgttaa
    17461 tccggttggt ggttaccagc ggcagcgaga cgtggccttt cagtttgcgc gtgacccagc
    17521 taaatgcgcc gcgcggcacg ggcgtggcga tggtcggaat ccgcgcttcg tgccagccta
    17581 ttccggtgtt gataatggtc gcgcccgccg cttcaatggc ctgcgccagt tctaccgttt
    17641 cggcaaaagt gccgccgcct tcgactaggt cgagcatcga caaccggtaa ataataatga
    17701 agtcgttgcc gacgcgttcg cgcaccgtac gcactacttc tacggcaaat cgcatccggt
    17761 tgcggtaatc gccgccccac tggtcactac gctgattggt gcgcagcgtc agaaattcgt
    17821 tgatcaaata cccttcagag cccatcactt cgacgccatc gtatccggcc tcccgcgcca
    17881 gttgcgcgca gcgggcgaaa tcgtcgatca gttgcaggat ctcttcatgg gtgagttcgt
    17941 gaggaacgaa acggttgatg ggggcctgca atgcggacgg tgcgaccaga tgcggttggt
    18001 agctgtagcg cccggtatgc aaaatttgca gggcgatttt gccgccttcc tgatgtacgg
    18061 cttcggtaat ggtgcgatgg tgtgggatct ggctggcgtc gttgagcatt gcgccacctt
    18121 ccatgccaac gcctgttaaa tctggtgcaa tgccgccgct gacaatcagc gccacgccgt
    18181 gacgggcgcg ttcggcataa aacgcagcca gccgttcagc accgtccggg tattcctcca
    18241 gcccggtgtg cattgaaccc atcaacacgc ggttttttaa cgtggtaaaa cctaaatcca
    18301 gcggggcgaa cagcgacggg tagctcataa aattgtccag tatgtaaaat aattgttatg
    18361 tagtcggatg agttctaatt tagccgtcga tggagaaaag ggaaaagggg agtgcggggt
    18421 ttgtgatggg attcaaaaag aatacgcccg gtggtaacgc cggatgcgac gcttgccgtg
    18481 ttttatccgg cttgggggat tgcacatgta gggcggataa ggcgttcacg ccgcatccgc
    18541 cagccgttgc aaattactgc ttacggaaaa aatcgttata caacatatac aaatgagcgg
    18601 cactccagga gaaatttggt gcgccttgct gcgcgcctgt cagcgggttg taattctcct
    18661 gaattgggcc atcggcggtt aatcctttgg cgtgcctgaa gaacgtatcc gccagcttca
    18721 gggcatcatc acgataaccg taacgctcca tccctttcag accaaaccag aactgatcca
    18781 cccatacgcg cccgcgccag tagatatcag cgccaaaagc cggattggtt aacgccgccg
    18841 ttcccagcgg aacaaatgtg ttgaactctt taggatcgag catcaccttc accaccgcgt
    18901 cggcattggc ctgcgttgcc gcaccgttaa acagcggcga ccagccttcc ggccctttac
    18961 cgcgctcaac aatcggtttg cccgcgcagc cgttcgccag tggtttatct tcaatacgta
    19021 cgtcatagta gaactgggta gtcgggtcga acatacaggt gttgatgtag tccgcgagct
    19081 gctgtgccaa ctggcgatag cgtttggcct cttctggctt accgaggatg gttgccatct
    19141 ccgccagata atggttatcg ctgtacatat agctggcctg atccaccgac tcctgcaata
    19201 gcgagtagcc cagcaacgtt ccgtcctgac tgcggttttc ggcgaatttc accgtccagt
    19261 cgctacgttt gccgccatta gcgacatatt tatccaactg ttctttgtcg ataaaaccaa
    19321 agacggcggc atcatcacgc cccgattccc acgacgcagc gacctgtgcc ggaatttcca
    19381 gactgtcgta ctggcctttc tccaccacgc gggcgtagtt gttcagccca gactgcgtct
    19441 cttctttatc gccttttttc accgtaaaca gcatctcgcc gctctcggtg ttgtgggctt
    19501 tgtcgcgggt cgcgccatat tccggcaccc cgttgccgtt atgatcgcgg ttacgtaacc
    19561 accagtcgtg ataggccacc agtttcgggt acatctctgc caaccaggct ttatcctggg
    19621 tgacgttgta tacttccatc accgaccagg cggcaaggct gggtttggta ttacgttcgt
    19681 tccagttgcc gccatcgcca ccacgctcgg ggctaaggtt ccacgctatc aggtcgggga
    19741 caaagcccac atcctgcgga cgcacgctgt cgccaggctg gatctgccag gagaagaccg
    19801 cgcggatatt ctctttggcg atatccggat tgaaatgcgc catcgcaaac gcctgcttcc
    19861 aggtatccca cggccaggtc tgattgccgg agaaccagcg tccggtcacc gacggcgtga
    19921 cggtgttata attcaccgca ccgcccggtg agcgccagtt accgttgagc gtttcgatcg
    19981 ctttcaccgc gacgcgcgtc tgttccggcg tcgcatccgg attggttaac cctttcttca
    20041 ggtattcttc ccagcgttgc tgcgaggcgg tgagataaaa cgccggacgc gccagaatat
    20101 cgcggatctg catttgctct ttgctaactt cctgagcggt cagcagatgg gaataggtgg
    20161 tgtagagcgt ggtcgaaccg ttgatatgcg ccttactggt aaagcgatta ccgttgattt
    20221 cagtctgcac cggcagggat ttatgcacct gatattccga ttcgccggag gtcagcagat
    20281 cccaggtggc gcgcactttg ccaaaggtga ctttcaggcc atcacgggtg gcgctgattt
    20341 tgcgctgata gtcagggtat tcgccagcaa tggttttatc ggaaagcggt ttcccttctt
    20401 tcgcttccag tttttccagc agttcgccat cccacaccag atccagcggt ttattgctga
    20461 tgattttagt ttccagtagt gacgtgcgcg gcgtggcgaa gcgcagagtc atttcgacct
    20521 gcacatcttt tgctgtcagt ttttgcacca gcgcgccggg aatactgtat gcctccagcg
    20581 tgaagttgac tttcttgccg tcttgccaga cagtcaggcg gtcgaaattg ctggccataa
    20641 agttgatgta ttcttccgtc agcagcgcaa cgcccggaaa gccgcccatg gtgttcgggc
    20701 cgtctggcaa cagatgacca tgccaggctc cgagatcgaa aaacggatta aaacgctggt
    20761 gatcgtcgta atcgtaatcc ttcatgtact gcggcgcacc agtacggtta atcacgtttt
    20821 tataattgtc ggcgttagcg gcatgagcgg aaaaacttat cagcagagcg caggttactg
    20881 gcgttaaaat agttttgatt ttcatgagat tacagtcctt attaccagta gaaatattgc
    20941 atcaggaaga tttggtcgct gggatcgtta gaggcatttt tcatgtagaa gcggctatca
    21001 aaagccgtgg cgaaacgtcc gccgccatat tcgtaccaga taccaaactg catgaaagag
    21061 gtggtttcat cgtcagcgtg gtcagggcga tgtttggcgt aggtgtaagc ggtggagaga
    21121 taaacgcctt ttaacgcttc atacatggcg ctcaccatca tcccgctttc acttcccggt
    21181 acgtccagcc cgtcggcacg tccggtatgg tgccaggcgc gagcggcaaa gttaggggta
    21241 aacaggcccg tgagatagag ttgtcctgtg ccgtcggtaa tttccagacc attcatccag
    21301 gtgatgcctt cggcaaggtc atactggatg taaccgttca tccattgccg gataggtgta
    21361 tttgtcgtcg tagcgatcgt acttgccaaa atgtagccag gctttgcttt cgtcatgatg
    21421 tccggcaggt gtggcggtga cgctataacg aaagttgccg cttaagttct gtaccttcag
    21481 cgcggtcata atgtcgcggg tattgggaat gacgtagccc aaatcgggtg tgaagtcgcc
    21541 ccaccattgc aggtcatcca gcgaggaatc ctgacgcaga ctgagcatga tttcggtgcc
    21601 atcattggta cgataaccgc catagaaacg gttaatccca ccttcgaaac caccccagct
    21661 atggtcaggc acctaggcat taccgtcgtg gtcggcctgc acggtccagc cttcgccgta
    21721 aatcaggccg aaccaggagc cgtacttcat ctccaggcca ccttcaatat aggtgccgtc
    21781 gctgtattta tgtttattct caccctctaa atccatatat ccaccaagcc ctaattcgcc
    21841 gtaaaaacga aatgaaggag aagatgtcga ggtaattgcg ggtgtttttg tgtcgtcatg
    21901 ttctgccgga cgtgcttctt ctcctgccgc agaaaaggcg tagcaaaacg gcagagcaag
    21961 taatgctctg catggtgcag tgattaattt cattgtgtta atccatgagc taaagaaaaa
    22021 gtacagcctt ttatcgttgt aattataaaa atataaaggc agaaactatt ttccctctcc
    22081 tcatccagga agagggaaaa tgcctgctca ttttttatta gtctgcttaa tcaacattgc
    22141 tggctggcgt cgcttcaata tggcgctctt cagccgttaa tccctttata tatttgctgt
    22201 atttccacca cgcaaagccg aggaagataa caataccgcc gacgttataa aaaatgatgg
    22261 tcagaatatt cgcgccagtc gggaatgtcg aagcgacaaa ccctacggca aatatcgcaa
    22321 tcagcattga aacaacgata ataccggtgc ggcgggagcc catacggaaa tcgcgtggca
    22381 ggtgatctaa tttggcgcgt aaattcaggt aagccagcat gatgaataac ggcggaagca
    22441 tggacgctgc ggcggtcata ttaataatgg tattcatcag atcctgcacg gtattggaac
    22501 cgagcatcgg gataatcatc agcgggatga cgatcagaaa ctggatccat gctgcgcgcg
    22561 ccggaacgcc gttttcgtta agttcgacgg ttttcttacc aaagatgcct tccgggattt
    22621 cggagaagaa aattttcacc ggcgttgcgg tccacatcag cagggaaccg aacattgcgg
    22681 taaaggacac cagaccgaca aagcgattca tcaacgcttc cggcagacca aaatacgccg
    22741 ccatgccgtg gaatacctgc accgatccgc cggtaaattt caactctttg ctgctgacga
    22801 agacgttaat cagcaccgag gagacggaat acagtacgcc gataaaaatc ccggcgagga
    22861 tgatcacttt aacgaacgat ttcgaaccgc ctttgacgtc gttaacgtac accgcgacgg
    22921 actccgcgcc acctgcggcc ataaagatcc aggtggtaac gccgaggaac gcccagttga
    22981 agttcgggat catcgcgtca acggtgatcg gatcggcagg ctgtacgccg ccaaccagtg
    23041 ccgtacctgc cagtaaaatg taggagagcg ttaacagcag catcagcgtt gaagtgacgg
    23101 aggtaattgg ccccagcatt ttcgccccgt tggtggaaac ccaggtggag aaggcgaaca
    23161 gcaccatact gataacggtg gtggcaaccg gcgtcataat atattcgtag ccgaggaagg
    23221 catacgaagc ataagcaata acgcgcggca acagcgaggt gaaaaagaac agatttacga
    23281 accagtaagt gtaggcagta ataaatgccc aacgtccgcc cagcgaactt tttacccacg
    23341 cgtagacacc ggcttctgag tttttattta acgaaacaaa ttctgcgatg atcagacaga
    23401 agggaataaa ataaaaaatc gtcgcgagga aaaacatcgg tgccgaggcc agtccaagct
    23461 caatattatt attgataacg ttgttaaagc tgtaaacggc ggcaaaagtc agcgagagca
    23521 agccgaattt gccgattgta ttacgtttgg tatcagacat aacacatctc cgaaaatgtg
    23581 tagggtagcg gtggcggcaa actccttttg ccgccgtagt tgttatttgt tatggaaata
    23641 accatcttcg atggtgactt tgagaaccac ttttttgacc gcgttattgc agataaaccg
    23701 atacgcttca tggatatcgc aaataacgat ttgcccttcg tggacctcta cggtttctcc
    23761 gcagcctttt aaatattcac ggtcagtttc atcacgataa tattccacta cctgtaatgt
    23821 ctctttcggc gcatattcaa ttttttgctg cccttgcagg tagtaatgca cttcaaaata
    23881 gcgtcgatgc ccggtaaata gcgcatcggt cgcggagtca ttttccacac ggtaggtcaa
    23941 tgaatcaccg atggagtggg cgacgccagg ctgaatgttg tcgatatttt caatcgcttc
    24001 aacgcagcgt tgccacttct tgccagaggc gtaaatctgg cggaactgtt ctaagttatc
    24061 gatgatcctc attgcttatt ctcgctgtgc aaattcgtgg aaaagaaccc tgcgccgaac
    24121 tcatacgacg ccaggctttg cgcggtagct tctccgccag aaaccggcag caacgtaaag
    24181 ccgtagctga agtcacggaa ccagacgcgc caggagtcca gcacctcgct gccccaggag
    24241 ttggagccga ggccaagtag ctggtggtcg aggttcaggg tgatgtcatc actgcgctgc
    24301 agctcgttac agtgctgggc agcgtggatg ttttcctggg tatagtgcca ggcgctgaag
    24361 ttaattgggc gctgcggaac caccagcaga ccgttgccgt ggcggttagt cagtgccgtc
    24421 cagcggacat gctgacggtt gccgttgttc tgcgggaagg gatagttctc gaacatggca
    24481 tcgacggtgc tgcgccagat atcgatgatg ttagcctgct ggctgtcggc gtagttttcg
    24541 cccggtccac gaccgtaata cgccacctga tcgtattcgc cgttaatccc catggtgaaa
    24601 ccgatgcacg gaatgatgtg cggatagtcg ccgtaacgct cgccggaaag cgccacgtta
    24661 acctggccat cggcagcgat gcgccagatg taggtacagc gcatcccgaa gtcaaacacc
    24721 ggcggtgcaa taactgtgcg gctgatgatc aacacttcac catcgctctg ttctacggca
    24781 aagtcgcgca gatgttcctg catgatctgc aaatgattcg gctgccacaa cccttcgtac
    24841 tcctgcttgt ggttgtcgat catcggcttg aagaagttga tctttggctc gcgggtcagc
    24901 agcgactcgc catttacctg ccaggatgtc ggtttgccac tcatttttga gaaggtgatc
    24961 gcgaagttgt agccgcgaac ggtgcagctc aaacgatcgt cttccagcgt cagcggacgc
    25021 gcattatttg gtgcgaaagg cactggctgc gcggtgtttt ccttcagcgg gaactgataa
    25081 gtggcgatag aatgcccggc ttcgctgtag cgggtgcggg aatctttggt caccgtaatg
    25141 ttgaggaacg cttcgcgggc gtccagctgc ggcagcgtga tctgcaaggg ggcttcgctg
    25201 ttcggcgcaa cgtcgcgcag tttaatctgc tgcgtcgcga gcgtttcacc ttcggcgcgc
    25261 acctctgcgt gcagggtgta gtcatcaagc gtggtaaacc acagtttatt ttcgactttc
    25321 aactcgccgc gagtcagcgc gtggattttt accggcgcga taacctgttt gtactctttc
    25381 aggcccgatc ccggcgtctg atcggaatag atcaaaccat caagacagaa gttatagttg
    25441 ttgggatagt cgccgtagtc gccgccgaat ttataccaga cattgccgtg gtcgtcctgt
    25501 gcctggatcc cgtggtcgca ccactcccag acataatgac cctgaatgca atcgtgctta
    25561 tagaagacgt tctggtactc cgtcagcccg cccggtccgt tccccatcgc atgagcatat
    25621 tcacagatga tgcgcggctt cggatgcggg tattcaccaa actcattcat cagcggcacg
    25681 cgggtgtaca tggtggaaat aatatcgacc acttcagcat cgcgatcttc ttcgtaatga
    25741 accagtcgcg tgtcatccag cgctttcgcc gcatggtaca tcgcgcggat gttacagcca
    25801 tagccggatt cattgcccag cgaccagatg atgatcgacg gatggttttt ctgtgcgtgg
    25861 atatggcgaa caatgcgctc gacgtagact ttttcccact gcggatcgtc ggtaatacgg
    25921 ctgatatcgc cgacattagc aaagccgtgc gattcgacgt cggtttccgc catcacaaac
    25981 aggccgtaga tatcacacag ttcgtaaaaa cgcggatcgt tcgggtagtg agcggtacgc
    26041 acggagttga tattgtgctg cttcatcaac tggagatctt tctcgacgcg atccattcca
    26101 acggcgcggc ctttgcgatg atcgttgtcg tgacggttga cgccgtgcag catcacataa
    26161 cggttattga tccagaacag accgtcgcgc actttgatat cacggaagcc aacgcgttgt
    26221 ggcaccactt ccagaacgtt gccgttggcg tctttcagcg tcatgaccag atggtaaaga
    26281 taaggggatt ctgctgacca ttgctgcggc tgttcgacag taaaagcaaa gctggcgctg
    26341 gtcagttttt caattgccaa atgatcaatg gcgctgctgt gcaccacgcg ttcgccatca
    26401 aacagggtat attccagcgt cgtgacgaca ggggaggcgg cgagattttc cagcaccact
    26461 tcgcaggaaa gcgtggcatc gcaataggct tcgtcaaagt cggtacgcac agtgaaatcg
    26521 ttaatatgcg ttaggtgttt tccgaccaga taaacatcgc ggaagatccc cgccgaccac
    26581 cacatatcct ggtcttccac gtaggtagag tccgcccact gcatcacgcg cacacacaac
    26641 aggttgtcgc cggttttaac catcgcgctg atgtcaaact ctgcggtcag gcgactgccc
    26701 ttgctgaaac ccacatactg accgttaacg tagacttcaa aataggtttc gacgccgtca
    26761 aatttaatca gcgtctgttt accctgccag ccgtcgctga gggtgaaaat acgttgatag
    26821 gcaccggttg ggttatcgct ggggacaaac ggcacatcga tggggaacgg aaaaccttcg
    26881 tcggtatatt gcagtttgcc gtgaccttcc atttgccaca tggcggggac ggtaatatgc
    26941 ccccagtcag ccattaactc agaggtgaag gcttctggta cttgcagcgg atggtcaaaa
    27001 aagtggaaat tccactgact gcttaagggc agaaacaggc tgctggtttc gcgggcaaag
    27061 gtacgcgctt gcgcaacaga atcatatgaa aaaaagtacg cacgcggcgc aagtcggttt
    27121 tcgtgggtga gctgaatgtt ttcccagcga ttcatagggc ctcccggtag agagaaatcg
    27181 gaaccattca tggtgatggt agtttgtgta attgctgggg gtttagtaaa atttttacta
    27241 aaaagcagca ggtaaatgca tttactttaa ctggatcacg aaaataaacg gcagcactgt
    27301 ccggcaaaaa tgattgccga acagtgtgta aggggattta acgggtcgtg ccgcgcagtt
    27361 ttaatttgct gggaacgaag actagcagcg gcagcgcgcg accatcgcgg gctttttcat
    27421 acaccaggtt aacgccctga cttcccatca tctcggaatg gatgcgcacg gtggagagcg
    27481 gcggaaaggt aaatcgcgcg gtggggatat cgttaacgct gataagcgaa atatcttgtg
    27541 ggatgttcag gccacgttca tgaattgccc gcagtacgcc gatagcaatg gaatcggaag
    27601 caacaaacag tgccttcgga tagtcttccc gcgccagcat ttgttttgcc agttcataac
    27661 ccgacgaact ggaaaaaccg ccgcgccaga tatcctcttc gcgtaccact tgtttcagtc
    27721 ggccatattc cgcaaaggcg acctcacgaa tatccgcctt gccaggctca tcttcaccgc
    27781 caataaaacc aatacgatta acgccctggt tgatatagaa gtcgatgatt tctttactga
    27841 tgcgtgccag atcgatatcc accgcatcgt aaccgctgcc gggttcgtga aagtcgataa
    27901 aacagatatt gtcggtcaac gcgctggcag cggcgcgcag ggcgggcgtg ggtttgccga
    27961 caattaaaat accggtgacg tttttaatgt ctggtaatcc gttgtgttca taacagttgg
    28021 tcagttcgat cgccagcttt tcgcactgag tttcaatgcc gtggcggatc gccagatagt
    28081 aaggatcgtt gatctccagc tcctgctggt agctgtagat agccagaata tggtgttggt
    28141 tgactgcgcc tgtctgtagt ttacgggcgc tactggtctt gtactccagc ttttcggcga
    28201 tctcgagaat gcgatgtttc gtctcttctt tcacattcaa tgtcggatcg tcatttaaga
    28261 ccctggatac tgtcgccagg gatacgccag cttcgattgc gatgtctttt aatgttgcca
    28321 tgtttacctt ttttactttc cctgatgtgc gcccccattg tagaggaagt gcttatcggg
    28381 tcacctgttt tcagtaaata aagcgcgttt ctttgacgct gtaacggtaa cacagcggct
    28441 taggaatgtg atcacgcctg cattctccat ttttagccta caggattgag aaaaactttt
    28501 ctatagttta gtaaaatttt actcgaaggg aggaagcaat ggaaaccgtg gcttacgctg
    28561 attttgcacg tctggaaatg cgcgtcggaa agattgtgga agtgaaacgc catgaaaacg
    28621 ccgacaagct gtacatcgta caggttgatg tggggaaaaa acactgcaaa ccgtgaccag
    28681 cctggtgccg tactacagcg aagaggagct gatggggaaa acggtggtgg tcttatgcaa
    28741 tctgcaaaaa gcgaaaatgc gtggtgaaac gtcggaatgc atgctgttgt gtgcggaaac
    28801 cgatgacggc agtgaaagcg tgttgttaac gccggagcgg atgatgcctg cgggcgtacg
    28861 cgtcgtataa aaagatcgga tggcgacgtc gtatcgccat ccgatttgat attacgcttc
    28921 ttcgacactt actcgcatgg ccgccagcgc cttacgcgcc gctttgatca ccagttcaca
    28981 ctgttcaatg gtcagtgtca gtggcggttc aatgcggatc gttttggcgt tattgagcgt
    29041 tccggccacc agtacgcgct ggcggaacat ctcgctggca aagttatagc cgatttcgtt
    29101 atcaacaaac tcaatcgcca tcaacatccc tttaccacgc gcttcctgta ccagatcggg
    29161 atattcccgc gccagttgac ggaaaccgtc cagcaacata tcgccttttt gctcagcctg
    29221 agccggtaag ttctgctcca gcaacacatt gatggtcgcc agcgccgccg cacaggccag
    29281 cgggttgccg ccaaaggtgg tggtatgcag gaatgggttg tcgaacagaa ctgaaaacac
    29341 ctcttcagtg gcgatggtcg cgccaatcgg catcacgccg ccgccgaggg cttttgccag
    29401 acacagaatg tccggctgaa cgttctcatg ttcgcaggca aacatcttgc ccgtgcgccc
    29461 catgcccgtt tgtacttcat cgaggatcat cagtgcgccg aactcatcgc acagcttacg
    29521 cacggcggtg agatagcccg gcggcggcag aattacgcca ccttcgccct gaatcggttc
    29581 gaggatcacc gcagccacat catcaccggt ttttttgcac tcattaagag ccgtgcgcat
    29641 ggcttcgata ttgccaaacg gcacatgacg gaaccctggc agcagtggca taaacggttt
    29701 gcggaaggtc gatttcgccg tggccgacag cgcgccaagt gatttaccgt ggaacacgcc
    29761 gctggtggca ataaaagtaa atttgccgcg cggcgactga taagccttcg ccagcttcag
    29821 cgccgcttca acggactcgg tgccgctatt acagaagaag ctgtatttca gtttaccggg
    29881 cgttagcgca gcaagggttt tcgccagcat agcccgtaac ggatcgagta gctcctggct
    29941 gtgcagtggt tgtttcgcaa gttgattctg tacggcggaa accacaactg gattacggtg
    30001 ccccacgttg aaaattccaa aacctcccag gcagtcgata aactcctgtc cctgggtgtc
    30061 gacaagcgta tttaaacctc ccgcttgcca ctctacggct ccgtaatccc cgccggcggt
    30121 aacagatttg cgatactcta aaaaccccgg attgacatgc tctttgaagt attcaatcac
    30181 ctctcggtta agtgctttca tctcctcatg atccagcgtt cgcttctcaa tgagattcag
    30241 ggcgtgggcg ctgcacgcta aagccgatgc gctcgaaggt aacctgttca aaatatgctc
    30301 cggaggctcg cgtatcacat gataccgatt taagtattgc agggattgcg ccactccggc
    30361 tgcgatcgcg aaaatcggga taaacacaaa tgcggttatc atttgtgcaa tatttaatgc
    30421 tatccgttaa catttgttgt tattttgcac ggtttttgtc gggtatggat ttgatgattg
    30481 ctgcgcaaat gcacgttttc agggcaataa ttgcctgatt tttgggcaag ataattaacg
    30541 ttttcgtata gtaacagagt gaaagtattt taaatcaaag aattagattt gtgctttaat
    30601 cgcaaattgc gatctaaatc aaattaatcg gttaaagata accgcagcgg ggccgacata
    30661 aactctgaca agaagttaac aaccatataa cctgcacagg acgcgaacat gtcttctcat
    30721 ccgtatgtca cccagcaaaa taccccgctg gcggacgata ccactctgat gtccactacc
    30781 gatctgcaaa gctatatcac tcatgctaat gacacttttg tgcaggtgag cggctttacc
    30841 ttgcaagagt tacaagggca gccgcacaac atggtgcgtc acccggatat gccaaaagcg
    30901 gcgtttgcgg atatgtggtt caccctgaaa aaaggggagc cctggagcgg catcgtgaaa
    30961 aatcgccgca aaaatggcga ccattattgg gtgcgggcca atgcggtacc gatggtgcgc
    31021 gagggaaaaa tcagtggcta tatgtcgatt cgtacccggg cgacggatga agagattgcg
    31081 gcggtggagc cgctgtacaa agcgttgaac tccggacgta ccagtaagcg tattcataaa
    31141 ggcctggtgg tgcgtaaagg ctggctgggt aaactgcctt cattaccgct tcgctggcgg
    31201 acgcgtggag tgatgaccct gatgtttatc ttgctggcgg ccatgctttg gtttgttgct
    31261 gccccggtgg tgacgtattt cctctgtgtg ttagtggtat tgttggcaag cgcttgtttt
    31321 gaatggcaga ttgtgcgccc gatagaaaat gtcgcccgtc aggcactgaa ggtggcgacc
    31381 ggagagcgta atagtgttga gcacctgaat cgcagcgatg agctggggct gacattacgc
    31441 gcggtagggc agcttggcct gatgtgccgt tggctaatta acgatgtctc aagccaggtg
    31501 tccagtgtca gaaatggcag tgagacgctg gcgaaaggca ccgatgaact gaacgaacat
    31561 acccagcaga cagttgataa cgttcagcaa acggtggcga ccatgaacca aatggcggcg
    31621 tcggtgaaac agaactctgc cacggcgtcg gctgccgata aactttctat caccgccagt
    31681 aatgcggcag tgcagggtgg ggaggcgatg accacggtga tcaagacaat ggacgatatc
    31741 gccgacagta cctagcgcat tggcaccatt acttcgctga ttaacgatat tgcgtttcag
    31801 accaatattc tggccctgaa tgcggcggtg gaagcggcgc gtgccggcga acagggcaaa
    31861 ggttttgcag tggtggcagg ggaagtgcgt catttagcca gccgcagcgc taatgctgcc
    31921 aacgatattc gcaagctgat tgatgccagt gctgataagg tgcaatccgg ttcgcagcag
    31981 gtacacgccg ccggacggac gatggaagat attgtggcac aggtgaaaaa cgtcacccag
    32041 ttgatcgccc agattagcca ttcaacgctg gaacaggccg atgggctttc cagcctgacc
    32101 cgtgcagtgg atgagcttaa cctgatcacc cagaaaaatg ccgagctggt ggaagagagt
    32161 gcgcaggtgt cggcgatggt gaaacaccgc gccagccgac tggaagacgc ggtgacggtg
    32221 ctgcattaat cggtatgccg gatccggcgg ttggagcaca atgcctgatg cgatgctggc
    32281 gcatcttatc aggcctgcgg ggtgtgtagc gggtgtaggc cggataaggc gcgtcaagcg
    32341 tcgcatccgg caattgcacc gcgccactgg cggatgcggc gattaatcca gttgcgtaat
    32401 atcaaaagcg gcgcggtcga tcaccgcaat gatctttttt atttgtgcat cactgatatc
    32461 gctctggttg acgcgtaaat ccagcaccgc tttaaaatta tccagcgccc gcttcatttg
    32521 cgggttctgg cgcagcgccg cgccaacgca acgcgctttg atgcgttctt caatcatctc
    32581 cacttgttcg cggttttctt ccagccactg cgcgccttgt tcggtcagcg caatctgctt
    32641 cttaccacct tcctcttcgc ggatggtaat cagcgactgc tcctgcagaa aatccagcgt
    32701 cgggtagatg acgcccgggc ttggggtgta attcccctgg gttagattct caatcgcttt
    32761 aatcaattcg taaccgtggc tgtcatcgcg cgagagaata tccagaatca ccagacgcaa
    32821 ttcaccgtga ccaaagaaac gttgccgacg accgccgccg cgaccgtggc gaccaccgca
    32881 acattgaccg tgttcatgct ggtgaccgtg tccgcagtgc tcgtgttctg acttctcacc
    32941 tttgcagcag ccctcatggc gtggctggcc ttcatgttta caacaccctt cgtgatgatg
    33001 gctcatatca gcctcctttt gcttgattag atatatctaa tttatatctg atgtgagcat
    33061 tactgcaagt acaaaaatga tttttaaacg tggaagcacc tcacatctag gccggataag
    33121 acgttcacgc cgcatccggc agcattgcta tggcactgaa tgccttcaca tacttcagac
    33181 atatcagttc tacaaatcgc ttgcatttat catgattaac aatcattatc atttgcgaat
    33241 tttatttaga tatatctgat taccatcacg aaggcgataa caatgaataa caccccccgc
    33301 tacccgcagc gcgttcgcaa tgatctgcgc ttccgtgaac tgactgtgct acgcgttgag
    33361 cgcatcagcg ccggttttca gcgcattgtc cttggcggcg aggcgttgga cggttttaca
    33421 tcgcgcggct ttgacgatca cagcaaactc ttctttcctc aacctgacgc tcactttgtg
    33481 ccgccaacgg taacggaaga gagcatcgtc tggccggaag gaccacgccc accgtcgcgt
    33541 gactatacgc cactgtatga cgaactacgc catgaactgg cgatcgattt ctttattcac
    33601 gacggtgggg tcgccagcgg ctgggcgata aacttacggt ggcaggtccg cgcggttcgc
    33661 tggtggtgcc ggaagattac gcgtatcagc tgtatgtctg cgatgaatcc ggaatgcccg
    33721 cactgcgccg ccgcctggaa acgttgagca aacttgccgt taaaccgcaa gttagtgcgc
    33781 tggttagcgt gcgggataac gcctgtcagg attatctcgc gcaccttgat ggttttaata
    33841 tcgaatggct ggcacatgat gagcaggcgg tagatgcgcg tctggcgcag atgcaaatcc
    33901 ctgccgatga ttacttcatc tggataaccg gcgaaggtaa agtcgttaag aatttaagcc
    33961 gccgctttga agcggaacag tatgacccac agcgggtacg tgcagcggct tactggcacg
    34021 caaagtaact gatatataaa aagaaaaagg ctgacgattt ctcgtcagcc tttgctatat
    34081 ctggtggccc ctgctggact tgaaccagcg accaagcgat tatgagtcgc ctgctctaac
    34141 cactgagcta aggggccatg gtagcggatt ataaagtaac tccgtgccgc aatccagcca
    34201 ttaccgcgcg cctgctgttt ttataaacaa tgtattttca atccgttata cttttcctgg
    34261 tgatgttaga aaggagtaaa tatggttgag gatattttgg ctccagggtt acgggtcgtg
    34321 ttttgcggta tcaaccctgg gctttcatcc gccgggactg gttttccctt tgctcatccg
    34381 gcaaatcgct tctggaaggt gatatatcag gccgggttta ccgaccgtca gttgaagccg
    34441 caggaggcac agcatctgct ggattatcgt tgtggcatca ccaaactggt agaccgtcca
    34501 acggtgcaag ccaatgaagt ttcaaagcag gagctgcacg caggtgggcg taagctgatt
    34561 gaaaaaattg aagattatca gccgcaggcg ttggctattc tgggcaaaca agcatatgaa
    34621 cagggattca gctagcgcgg tgcacagtgg gggaaacaaa cgctcaccat tggttcgacg
    34681 cagatttggg tgctgccaaa tcccagcggt ttaagtcgcg tttcactgga gaaactggtt
    34741 gaagcgtatc gcgagctgga ccaggcgctg gtagtgcgtg ggcgataa
//
LOCUS       DAJTSA010000034        34745 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000034 DAJTSA010000000
VERSION     DAJTSA010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34745
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.034"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(30..105)
                     /locus_tag="OO369_001794"
     tRNA            complement(30..105)
                     /locus_tag="OO369_001794"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(70..72),aa:His,seq:gtg)
     gene            complement(164..240)
                     /locus_tag="OO369_001795"
     tRNA            complement(164..240)
                     /locus_tag="OO369_001795"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Arg,seq:ccg)
     gene            complement(343..1728)
                     /locus_tag="OO369_001796"
     CDS             complement(343..1728)
                     /locus_tag="OO369_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5982097.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1919..2659)
                     /gene="rffM"
                     /locus_tag="OO369_001797"
     CDS             complement(1919..2659)
                     /gene="rffM"
                     /locus_tag="OO369_001797"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR5982098.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2662..4014)
                     /gene="wzyE"
                     /locus_tag="OO369_001798"
     CDS             complement(2662..4014)
                     /gene="wzyE"
                     /locus_tag="OO369_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR5982099.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(4011..5090)
                     /gene="rffT"
                     /locus_tag="OO369_001799"
                     /gene_synonym="wecF"
     CDS             complement(4011..5090)
                     /gene="rffT"
                     /locus_tag="OO369_001799"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR5982100.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5087..6337)
                     /gene="wzxE"
                     /locus_tag="OO369_001800"
     CDS             complement(5087..6337)
                     /gene="wzxE"
                     /locus_tag="OO369_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR5982101.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6339..7469)
                     /gene="rffA"
                     /locus_tag="OO369_001801"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6339..7469)
                     /gene="rffA"
                     /locus_tag="OO369_001801"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR5982102.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7474..8148)
                     /gene="rffC"
                     /locus_tag="OO369_001802"
                     /gene_synonym="wecD"
     CDS             complement(7474..8148)
                     /gene="rffC"
                     /locus_tag="OO369_001802"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR5982103.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8126..9007)
                     /gene="rfbA"
                     /locus_tag="OO369_001803"
     CDS             complement(8126..9007)
                     /gene="rfbA"
                     /locus_tag="OO369_001803"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5982104.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9026..10093)
                     /gene="rffG"
                     /locus_tag="OO369_001804"
     CDS             complement(9026..10093)
                     /gene="rffG"
                     /locus_tag="OO369_001804"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5982105.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10093..11355)
                     /gene="wecC"
                     /locus_tag="OO369_001805"
     CDS             complement(10093..11355)
                     /gene="wecC"
                     /locus_tag="OO369_001805"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR5982106.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11352..12482)
                     /gene="wecB"
                     /locus_tag="OO369_001806"
     CDS             complement(11352..12482)
                     /gene="wecB"
                     /locus_tag="OO369_001806"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR5982107.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12538..13584)
                     /gene="wzzE"
                     /locus_tag="OO369_001807"
     CDS             complement(12538..13584)
                     /gene="wzzE"
                     /locus_tag="OO369_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR5982108.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13596..14699)
                     /gene="wecA"
                     /locus_tag="OO369_001808"
     CDS             complement(13596..14699)
                     /gene="wecA"
                     /locus_tag="OO369_001808"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR5982109.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14939..16198)
                     /gene="rho"
                     /locus_tag="OO369_001809"
     CDS             complement(14939..16198)
                     /gene="rho"
                     /locus_tag="OO369_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR5982110.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16283..16384)
                     /gene="rhoL"
                     /locus_tag="OO369_001810"
     CDS             complement(16283..16384)
                     /gene="rhoL"
                     /locus_tag="OO369_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR5982111.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16525..16854)
                     /gene="trxA"
                     /locus_tag="OO369_001811"
     CDS             complement(16525..16854)
                     /gene="trxA"
                     /locus_tag="OO369_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR5982112.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16985..18250
                     /gene="rhlB"
                     /locus_tag="OO369_001812"
     CDS             16985..18250
                     /gene="rhlB"
                     /locus_tag="OO369_001812"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR5982113.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18384..19868
                     /gene="gppA"
                     /locus_tag="OO369_001813"
     CDS             18384..19868
                     /gene="gppA"
                     /locus_tag="OO369_001813"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR5982114.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(19915..21936)
                     /gene="rep"
                     /locus_tag="OO369_001814"
     CDS             complement(19915..21936)
                     /gene="rep"
                     /locus_tag="OO369_001814"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR5982115.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            22023..22304
                     /gene="ppiC"
                     /locus_tag="OO369_001815"
     CDS             22023..22304
                     /gene="ppiC"
                     /locus_tag="OO369_001815"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR5982116.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(22350..23825)
                     /gene="ilvC"
                     /locus_tag="OO369_001816"
     CDS             complement(22350..23825)
                     /gene="ilvC"
                     /locus_tag="OO369_001816"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR5982117.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            23975..24865
                     /gene="ilvY"
                     /locus_tag="OO369_001817"
     CDS             23975..24865
                     /gene="ilvY"
                     /locus_tag="OO369_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR5982118.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(24862..26406)
                     /gene="ilvA"
                     /locus_tag="OO369_001818"
     CDS             complement(24862..26406)
                     /gene="ilvA"
                     /locus_tag="OO369_001818"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR5982119.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(26409..28259)
                     /gene="ilvD"
                     /locus_tag="OO369_001819"
     CDS             complement(26409..28259)
                     /gene="ilvD"
                     /locus_tag="OO369_001819"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR5982120.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(28324..29253)
                     /gene="ilvE"
                     /locus_tag="OO369_001820"
     CDS             complement(28324..29253)
                     /gene="ilvE"
                     /locus_tag="OO369_001820"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR5982121.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(29273..29536)
                     /gene="ilvM"
                     /locus_tag="OO369_001821"
     CDS             complement(29273..29536)
                     /gene="ilvM"
                     /locus_tag="OO369_001821"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR5982122.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(29533..31179)
                     /locus_tag="OO369_001822"
     CDS             complement(29533..31179)
                     /locus_tag="OO369_001822"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR5982123.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(31182..31232)
                     /locus_tag="OO369_001823"
     CDS             complement(31182..31232)
                     /locus_tag="OO369_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5982124.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(31318..31416)
                     /gene="ilvL"
                     /locus_tag="OO369_001824"
     CDS             complement(31318..31416)
                     /gene="ilvL"
                     /locus_tag="OO369_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR5982125.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            31768..33247
                     /locus_tag="OO369_001825"
                     /pseudo
     CDS             31768..33247
                     /locus_tag="OO369_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(33272..33610)
                     /gene="maoP"
                     /locus_tag="OO369_001826"
     CDS             complement(33272..33610)
                     /gene="maoP"
                     /locus_tag="OO369_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR5982126.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            33729..34568
                     /gene="hdfR"
                     /locus_tag="OO369_001827"
     CDS             33729..34568
                     /gene="hdfR"
                     /locus_tag="OO369_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR5982127.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(34664..34739)
                     /locus_tag="OO369_001828"
     tRNA            complement(34664..34739)
                     /locus_tag="OO369_001828"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34704..34706),aa:Trp,seq:cca)
BASE COUNT         8477 a         9503 c         8772 g         7993 t
ORIGIN      
        1 caccttcgca ttgtcacaac ttctaataat ggggtggcta atgggattcg aacccacgac
       61 aactggaatc acaatccagg gctctaccaa ctgagctata gccaccacta caaatcttgt
      121 tacgcggtat tactaccacc gcagctcaag cgctgggact aaatggcgcg cccgacagga
      181 ttcgaacctg agacctctgc ctccggaggg cagcgctcta tccagctgag ctacgggcgc
      241 ttagcgccgt tgcgggggtg gataatacgg acttcccacc cctctgtcta gtcccttttt
      301 aaataaattg ctcgtttggt tacggtttgt gcgttttgct gcttattcct ccagtttatg
      361 cgctttaccg tggcgattaa gcccaaaaac tttataaatc gccgtcaccg ccagcataaa
      421 gatgatgccg acaaacagcg acatacgcgt atcttcatta aagtacatgc cgatcaaaac
      481 gcaaatcagg aatgccattg ttacgtagtt ggcccacggg aacaggattg agcggaacgg
      541 atggctggca atcgccgctt tatgtgcacg ccgaaaacgc agctggctta tcaatatcac
      601 aaaccatggc accatccccg gaagcacgct ggcactgtag acgtagacaa acacacgctg
      661 cggattggga atgatgtagt tcaggcatga gccaattagc agaatagcaa tagatactgc
      721 cacacccgca accggtacgc cgtgacggga aacttttgcc attgccgccg gtaactgacg
      781 gtttttcgcc agtgcgtaga gcatacgtcc gcaactgtac atgccgctgt tacagccaga
      841 gagcgcagcc gtcagcacca caaagttgat aatgcccgcc gctgcggtaa taccgatttt
      901 ggcaaaagtc agtacgaacg ggctgccgtt gctgccaatt tcattccacg ggaagatggt
      961 gacgataacg aaaatcgcgc ctacgtagaa aatcaggatc cgccacagca ctttgcctac
     1021 ggcactgcgc agcgtcacct gcggattctt cgcttcaccg gcagtaatgc caatcagctc
     1081 cacgccctgg taggacgcca ccacaataca cagagcggtc aggaaccctt tccagccacc
     1141 cgcaaagaaa ccgccatgct ctgtgagatt gctaaaacca atcgactgcc cgccattgcc
     1201 aaagccaaag aaaatcacgc ccaggccaat gacaatcatc acgataatcg tggtgacttt
     1261 gatcatcgcg aaccagaact cgatttcgcc gtacaaccgt accgccgcca gattcgccaa
     1321 cgccaccagc gccaccgcga tcaatgcggg tatccactgc gccatctccg gggaccaaaa
     1381 ctggacataa acgccaatgg cggtgatttc tgagatcccc accgccatcc acataaacca
     1441 gtaagaccag gcggtgagat agccaaaaaa cgggctcata taacgatgcg cataaacggc
     1501 gaacgaaccg gtaaccggtt cgaggaacaa catttcgccc attgaacgca tgatgaaaaa
     1561 aacgaacagc ccggcgatga tataggccaa caatacggat ggcccggccc atttcagggt
     1621 actggcggcc cccataaaca ggccgacgcc aatggtgccc cccagggcga tgagttcgat
     1681 atgtcgagct tccagcccac gctgtagctc tggtttgtta tctgccataa atcctcgtgt
     1741 tgtgtttgca tgctttccgg tgttaccggt tatcgttatg ggtacatcga gtgttgcgaa
     1801 tgttttcgta attcaggaga aatggcaaat aaagcattaa aaatttgaat gctttgtgta
     1861 ataaaaaagc agacaggcga cgaagtgacc actccgtcgc tttacaaaga gaggaaaatc
     1921 agaggttgcc ggtgtagtgc cagcgtaaat aacgcagcaa acgaagctga cgcttaatgc
     1981 ggctcggctg cgaaagcagg cggtagagcc actccagtcc cagcgtttgc cagattttcg
     2041 gtgcgcgttt tacgtgaccg gtgaaaacat cgtaagtccc gccaacgccc atatacagcg
     2101 catctggatg caccagacgg cagtcgcgca tgaagatctc ctgctttggc gatcccatcg
     2161 caacggtgac gatttgcgct ccgctggcat gaatgcgttc aaacagcgcc tgacgctgct
     2221 cgggtttaaa ataaccatcc tgactgccaa cgatattcac attccattgg ttgcgcagtt
     2281 tagcttcagt ttgcgccagc acttcaggtt taccgcccac aagaaatacc ggcgtccctt
     2341 ctttgcctgc gcgcgccatc agctcttccc agagatcggc accggcaacg cgggaaacct
     2401 gcgcctgcgg gtacttttta cgtactgaac gtacaatgct gatgccatcc gcatatttaa
     2461 attcggcagc gttaattaac tccctgacct cggcgttatc ttcaatggtc agcatttttt
     2521 cggcattaat ggcaaccagc gttccctgct taagctgccc gtcagcaaac agataatcga
     2581 gggcatgctg catatcacgc caaccaatca actgtaagcc acgcagcgta taggtcggtg
     2641 ccgtggtgtt gttattcatt gttatccttc aacctgcgtc cggagcgatg attttgtacg
     2701 tttatgaatg agtccggcgc tttcaaaaag ccagtacaac agttttgcga tcatcagaca
     2761 tgcgccgaag accacgataa aaaagactac gcgtgagaca aacgaatcca gcccttcacg
     2821 tgccagcacg atcatattga agatcgcccc aaagcagaaa ctgtgcaata tcgcagcctt
     2881 atagcgatta gtctcgcggt tgcccagctc atacagccag tcgaaccatt tgatgatcag
     2941 tccaaccaca atcgccccga gtgggatgaa caacgcgccg cccatcacca ccagtgagcc
     3001 tataagcgta ggcgagatcg ccagtccgga gtggttattc agtacttccc aggtaaagta
     3061 attggctgag ttcagcacca tactcgggcg ccccggccac agccaggaag ggataaagac
     3121 atagaaatcg cggacaattg gagccaggcc ctggaagtcg atgttgtcgt agttctgcaa
     3181 cagcaacgcc agattctccc acggtgaaaa ggtgtcgcga gtgagataga gaaacgtata
     3241 gaacgcttca tcgccgctca cattcattcc atagcgtttt aacgccagcc agaacatgcc
     3301 aacaatcccc agcacgcccg ccgccgccag catccacaac gaaatccagc cgcgaataat
     3361 gccaataaac aggaagatag cgaatgcgat gatgatattg gcgcgagtgc cgccgacaat
     3421 catataagtc agcaagccaa aggcgaccgt gctgacgagg aaaaacagcc acgctttgct
     3481 gtcctggcgc agaaagtaga ccaccagcat cgccgggatg aaaaagtaaa agaagcgttt
     3541 taacgccacg ccggagactt cactggaaaa gatctgactg taggagttaa gccggaacag
     3601 caaaaagccg ttgtgcatga agaagatgcc gacgcttacc agcgcgatac ccatcaggat
     3661 cacccacgta agattggtct ccacgcggtt catggtaaac agcggacggc gcggtacatc
     3721 agcaacgcgt ttgcgtaggc gggttttgta ggtgacatag taaaccgcgt agaagcagcc
     3781 cgcagaaagc aacacctgca acaagatttc tggcggcgcg acaccaacat caaagcgaaa
     3841 taccagcacg ctggtcagcg ggaagccgaa gaaaaaggtg agcaaaaaca gcaatgaaaa
     3901 gaagacattg aagttaaagc gcacacggcg aaactcaaac caggtcagcg tggcaataaa
     3961 cagcgtgcag agcagccaga ccacaaacag gccactgaat tgcagcagac tcatgcgacc
     4021 tccccggcgg caatcgccaa cgcccgctgc cagccttgta gatagttagg gctaaagaag
     4081 gcaatggtgt ttttatccgc cgacgccaac tggcgctgcg cttcacgcac aatatcctcg
     4141 ttgagatcgt cggtagtaaa cagcactggc aaatgctgtt ccgtcatatc ctgccagaac
     4201 ggattttccc tgttaagcac acaaggaatg cccgcctgaa tcagtaagca aagcgtacca
     4261 atgccctgct ggcgggcaaa aataaagtaa ccaagatcgc actgacgaag tagcgccaga
     4321 taggcgtcaa attccagttt ttcgctcaaa atttgcagat tttcttcgct gaataactcc
     4381 agccccgcct gacgaacttc ctcaatgtac gcttcgttat taggcggata tcccatcggc
     4441 accaccactt ttaccgtatc gccaaattgc tgatgaacgg cacgtaaagc ggcgacatgc
     4501 tcattgctgc ggtcgccgga gttccccacc agaatggtca tttttccttc acgttgccga
     4561 tcgttcgcca tcgtattgag agaagggtcc atccgcgtcg ggaagtacag cagttctccc
     4621 cgcacctttg ggtgtgtttt ggcaaaaaag ctcaaatcgc cgcgggtggc aaatacacag
     4681 ccgactcgct tttgcgccag gcgacgtagt gggtaaaaaa gcttatatct caagccactg
     4741 gaaagctcat acaggtctgc cccccagata tgccagaaaa actggctggg cttaatgcca
     4801 ccactcagca gagccagcca cagtgtggga ttgaactgac cgtggaaaaa aaatcgctgc
     4861 tgacggttag cttttgcttt cgcgatgacc gcttccgcca gcgatttttt cccagggaaa
     4921 aattgcacag aaagcgccgg acagctatca cttaagccgt cgtccttgcc aacaaccata
     4981 aactcgcgcg cgtgctcgct cgtcgcggcc agcgcgtcat tgaaaaaccg caaaacggtt
     5041 cggttatggt gagggatatc cgatcccagt acgtgaatca gtacagtcat gcccgcctac
     5101 gccagagtaa aaacacgcca caacaaagag aaaaatagac gatataagtt gccatatatg
     5161 cctgcgccgc gcccagtgca ccatgcgcag ggattagcca gtgggcaaat accatcaata
     5221 aagtgaactg gctgacttcc gccagaatat aaaaccgcag tgacgctttg gcgatcacca
     5281 gataaccaaa gacataagcg cccactttta acacatcacc cactaactgc caggcaaaga
     5341 gatcgcgcat agcggtaaat ttattcgaca acagcaacca gatagcaaaa tcacgcagca
     5401 accagacggt aaaactcgct gccgccactg ccggtaagac gaatttcagc gatttaacca
     5461 cttcccgggt gatatcgcgc ttttccgtta gccgcgacaa cgtgggcagc aaatatacgc
     5521 tgaacgatgc cgtaataaat tgcaggtagg catcggaaat actgctcacc ccttgccaga
     5581 tccccacttc atcccagcta tactgcgccg ccagcagttt acgcatcatg atgtaagcaa
     5641 caggcaaggt caccgacgta atcaacgcca tgagcgtaaa tttgctcaac tgccctgcca
     5701 gaccgttatc ccagctgggt ttcagatagc ttaacgggat gacaccacgt ttaatcaaca
     5761 tgatggcggc aggaattacc accagcgcgg gaatcagcgc cagacccagc aacgcccctt
     5821 cataaccgcc caaacggtaa ctgacgtagt acgcgagaac gccaatcaag ctgccgacaa
     5881 tcagcgataa cgcattacct gcggcatcgc gaaagccttt catcagcgcc agtaacaggt
     5941 tgccccaggc gatccccatt tgcaccagcg ccactaaacg caccagcccc tgatagtcgg
     6001 tattaccaaa caatccctgg ctgattggcg cagctgccag cacaaaaacc agcgccatca
     6061 gcgtagagaa accaagtacc atcgctgatg aagtgccgac cacgcggcgc agctgttgcg
     6121 gattatcatg gtactgggca acgtatttgg ttacaccgtt aaagatgcca gccccggcaa
     6181 gcacgccgag cacggtaatc aactggcgga aatttgccgc cagcccaagc cccgccggac
     6241 caaatgacac cgccagcaac ttaccgacca gtaacccggc accaatcttg accagtgtac
     6301 tggccgccgt ccacaaggac gcttttgcca acgacatatc aggagaagta gttcaacaaa
     6361 gtcgcaatta ccgtacgctg attgacgggc gacaggttgt agaacagcgg caggcgcagc
     6421 aggcgctcgc tctctttggt ggtgtagcga tcttcaccgt ggaactcacc aaagtgttcc
     6481 cccgcagggc aaccgtgcag cggaatgtaa tgaaacaccg ccatgatttc cgcttctttc
     6541 agaaagttaa tcaacgcgct ccggtcatca atatcccgca gtttaatgta gaacatatgc
     6601 gcgttctgca cgcagccatc gggaatcgac ggcagctcga tacgcccggc tttcgccaga
     6661 ggcgctaacg tatcgtagta gttttgccac agcgccagac gttgctggtt gatacgatcc
     6721 gctgcttcca gttgcgccca caggtacgca gcttgcagat cggacatcaa atagctggag
     6781 ccaatatcgc gccaggtata tttatcgacc tgaccacgga agaactggct gcggttagtg
     6841 cccttttcac ggatgatctc ggctcgttcg attaacgctt tatcgttaat cagcgtcgcg
     6901 ccgccttcac cacccgccgt gtagtttttg gtttcatgga agctaaagca gccaatatga
     6961 ccaatggttc ccagtgcacg ccctttgtaa gtggacatca cgccctgagc ggcatcttcc
     7021 accacaaaca aattatgctt tttcgccaac gccataatgg tgtccatttc gcaggccaca
     7081 cccgcgtaat ggaccggcac gataacgcgc gttttgtcgg tgatcgctgc ttcaatcaac
     7141 gtttcgtcga tgttcatggt gtccgggcga acatccacaa agacgatttt cgcgccacgc
     7201 agcacaaagg cattggcggt ggagacaaag gtgtagctcg gcatgatcac ttcatcgcca
     7261 ggctggatat cgagcagcag cgccgccatc tccagcgaag cggtgcagga cggcgtcagt
     7321 aacactttgg cgctgccaaa acgttgctcc agccactgct ggcagcgacg ggtaaaaccg
     7381 ccatcgccac acagtttgcc gctacccatt gccgactgca tatagtcgag ttcggttccc
     7441 accaccggcg gtgcgttaaa tggaatcatg tgatcacctg tataaccagt acgcggtgct
     7501 ttctacattc gcaccacttt gtatgtatcg tttaagcgcg gcggtgttgc ccatttgggt
     7561 cgccacccgc aaagttgttt taccgcgagc atacgcccag tttagcgccg tttgcatcag
     7621 ctcagcacct gcaccgcgtc cagccagcag gccaattcgc gcatctgtcg cattgagttc
     7681 ccgtaaagag acatagccgc gaatatcgcc ggacgccgca cgtagaatca gacattgatg
     7741 atcaaaggtg ccgcgcacgg cattttcaat ccactgtgca taaaagcgac cgctggcgtc
     7801 aggcgcatac cacggcgcac gaaaacggct ttgcgcaaat gcggcgctag ctaactgacg
     7861 taatgcggga atatcggtct cttgtgccac gacagcaccg ctatcactga cattgttcac
     7921 gggtagcgcc aaatcaactt caccttctac cagggagaat cccagctgtt gcagggcatc
     7981 cagttccccc gtatttgatg ccgcaatttt ggcctgcacc cgtgaccatg gcgctaaggc
     8041 gtctgacgtc aggagcggtg cttcagacgt aatgcgcacg atggcgctgt taacaccaaa
     8101 gaaggcgttt tcccaggtta gtggctcaat actggcgcgg acgggcacga agtaactcca
     8161 gcagatattg gccgtagcca gttttcgcta atgaactggc agcacgcttc acaccctcgt
     8221 catcgagcca gccgttacgc caggcgatct cttccaggca ggcaatctta aagccctggc
     8281 gtttttctac tgtctgcaca aaagtgctgg cctcaatcag gctgtcgtga gtgccggtat
     8341 ccagccaggc aaatccgcgc ccgagcagtt caacggtcag gttgcccgcc tcgaggtaca
     8401 tctggttgat ggaggtaatc tccagttcac cgcgctccga cggcttcacc tgctttgcgt
     8461 actccacgac tttactgtcg tagaaataaa gcccggtcac cgcccagttt gacttcggct
     8521 gttttggctt ttcttcaagc gagatagcgc ggaaattgtc gtcaaattcc accacgccaa
     8581 agcgttccgg gtccatcacc tgatagccaa aaaccgtcgc cccttccgtg cgcgccgcaa
     8641 catgacgcag cttcggactg aagccctgac cgaagaagat gttatcgccc agcaccagac
     8701 aagaaggttc accattaagg aaggtttcac cgatgataaa ggcctgcgcc agaccgtccg
     8761 ggctgggctg ttcggcatat tccagctgga taccgaactc actaccatcg cccagcaggc
     8821 gctggaaata acctttatct tccggcgtag tgatgatgag aatttcgcgg ataccggcca
     8881 gcatcagcac cgacagcgga tagtaaatca ttggcttatc gtaaatcggc aacagttgct
     8941 tcgatacgcc gcgcgtaatc ggatgcaatc gggtaccgga accgcccgcc aggataatac
     9001 ccttcatgtt tgcctccgct gaaaattagc ctttcagacc taaacgctca ccctgatagc
     9061 tgccgtcctg cacctgcttc caccagcttt cattagccag ataccactga accgttttac
     9121 gcattccact ttcaaaggtt tcctgcggca gccagccaag ttcacgggca attttcgaag
     9181 catcaatggc ataacgcaga tcatgccccg gacggtcagc gacaaaggtg atcaggtcac
     9241 gatagtacgc cacgccgtgc ggcttgttcg aagccagttc ttccagcagc tcgcaaatgg
     9301 tttccacaac atcgagattc ttacgctcgt tgtgaccacc aatattatag gtttcaccga
     9361 ctttcccggt ggtcgccacg caatacagcg cgcgggcgtg atcttccaca tacagccagt
     9421 cacggatttg ctgcccgttg ccatataccg gcagcggttt acccgccagc gcgttgagga
     9481 tcatcagcgg gatcagtttt tccggaaagt ggtaaggacc gtagttattc gagcagttgg
     9541 tgatcagcgt cggcagaccg taggtccgca gccaggcgcg caccagatgg tcgctgctgg
     9601 cttttgacgc ggaataaggg ctgctcggcg catacggcgt ggtttcggtg aagaaatcat
     9661 ccgtcgagtg caggtcacca tatacttcgt cggtggagat atgatgaaaa cggaacgctg
     9721 attttttatc ttccgtcagc gtattccagt aagcccgcgc cgcttcaagc aatgtataag
     9781 tcccgacaat gttggtttca ataaacgctg ccgggccgtc aatagaacgg tcaacatggc
     9841 tttcggctgc cagatgcatg acacagtctg gctgatgctc agtgaatacg cgtgccagtt
     9901 ctgcccgatc gcagatatca actttctcaa aggcaaagcg ctcgctttgc gcgaccggtg
     9961 ccagcgacat caggtttccg gcgtaggtca gcttatcgac cactaccacc gcatcgctcg
    10021 tttcgttgat gatataacgc accagcgccg agccaataaa cccggcacca cctgttatca
    10081 gaatttttct catcagcgcc agactccttt ggcatcgacg acatactgct gatggacatt
    10141 gtcgccattg ataactttga actgactatg atcgaccagc atcaccagca cgtctgccgt
    10201 tgccagcgcc tcgtcaagct gcgccagagt acaaagcccg gtcagttttt tcggcaactg
    10261 gtggatgtta ggctcaacaa ccagcgtttc gccgctgtgc cactgggcga tcagttcagc
    10321 gatttccatc gccgggcttt cgcgcaggtc atcaatattc ggtttaaacg ccagaccaaa
    10381 gcaggcgatt ttcagttcac tggctcgttt atcggtagca gcaaggcagt cggcaaccgc
    10441 cgctttcacc tgatcgataa cccagaacgg tttgtgatcg ttaacttcgc gcgcggtacg
    10501 gataagccgc gcctgctggg ggttctgtgc cacgataaac cacggatcaa cagcaatgca
    10561 gtgaccgccc acgccagggc caggctgaag aatattaacg cgagggtgac gattcgccag
    10621 gcgaatcagt tcccagacgt taatcccctg atcggcacaa atcagcgaca attcattagc
    10681 aaaagcgata ttcacatcgc ggaagctgtt ttcggtgagc ttacacattt ccgccgtccg
    10741 cgagttagtg acgacacact caccttcgag gaaaattttg tacaattcgc tggcgcgggc
    10801 cgaacaaacc ggcgtcatac caccaatcac gcgatcgttt ttaatcagct cgaccataac
    10861 ctggcccggt aatacacgtt ccgggcagta agcaatgttg acgtccgcct gctcgcccac
    10921 ctgctgtggg aaagtcagat ccgggcgcat ctctgctaac cattctgcca tcttctcggt
    10981 tgaccccacc ggcgaggtgg attcaaggat caccagcgcg ccttttttca gcactggcgc
    11041 aatggagcga gcagccgatt caacgtaggt catatctggc tcatgatcgc ctttaaacgg
    11101 cgtaggtaca gcaatcagcc aggcatccgc ttcaactggc gtcgtgctcg ctcgtaaaaa
    11161 accgccttct acggcagttt ttactacgct cgccaaatca ggctcgacga tatggatttc
    11221 gccacgattg atggtatcaa ccgcgtgttg gttgatatcg acaccaatca cctgtttttg
    11281 ccgtgaggca aacgctgctg ctgtaggcag cccgatgtaa cccagtccga taacagaaat
    11341 ggtcgcaaaa ctcatagtga tatccgatta ttttttaacg cttccagaat gcgagagcat
    11401 gcctgaccat caccatacgg gttatgggcg cggctcatag cttgatattc gttttcgtct
    11461 tttaaaagac gcgtcacttc ctcgacaatt cgctgcttat ccgtgcctac cagacgcacc
    11521 gtacccgccg tcaccgcttc cggacgctca gtggtatcgc gcatcaccag caccggtttc
    11581 cccagcgaag gcgcttcttc ctgaatgccg cctgagtcag tcaaaatcag ccaggcgtgg
    11641 ttcatcagcc agacaaacgg taaatactcc tggggatcga tcagaatgac atttttcaca
    11701 tgccccagaa tgcgattgac cggctctctg acgttcgggt tgagatgcac cggatagaca
    11761 atctggatgt cctggtgcgt ggtggcgatg tctgccagcg cgtggcagat ttcttcaaag
    11821 ccacgaccga aactctcacg cctatgaccg gtcaccagaa tcatcttttt atcgggggcg
    11881 ataaacgggt aatttgccgc cagttctgaa cgcagcgtgt cgctgctcat cacctggtca
    11941 cgcacccata acagtgcatc aatgactgta ttaccggtaa tgaagattcg gctatccgca
    12001 acgttttcac gcagcaagtt ttgccgggaa gtttcggttg gagagaagtg atacatcgcc
    12061 agatgcccgg tcaatgtacg gttagcctct tccggccacg gcgaatagag agcgcccgtg
    12121 cgcagaccag cctcaacgtg accaacagga atacgctgat aaaacgccgc caggctggtt
    12181 gccagcgtcg tcgtcgtatc gccgtgaacc agcacgacgt ctggtttgaa ctcggcaaga
    12241 ataggtttta gcccttccag aatccgacag gttatctctg tcaggccctg tcctggctgc
    12301 attatgttga gatcgtagtc aggtacaatg gaaaagagtt tcagtacctg atcaagcatc
    12361 tcccgatgct gcgcagtgac gcaaacttta gcctcaaaaa aaggatcttt tgccaacgca
    12421 tgcaccaacg gagccatctt gatggcttcc gggcgcgtac caaatacagt cagtactttc
    12481 acatcgattc tcttcgaata agcggcgagc gcctttgcgc tcaccgcagc agtgttgcta
    12541 tttcgagcaa cggcgggtta atgcgacacc agccccgatc agccccccga caatgcccca
    12601 cataatcatc aggaaggcac gacgtgggct atcgcgtttt accggttctt ccggcgtacg
    12661 caaatagcga taggtctgaa aacgcggatc cagggttgga ccaacattca gggtgtttaa
    12721 catggctcga ttctgatcat agtcgagatc aaaggccgga ccgacggcct gtaaattttc
    12781 cagtcgagcc tggagcattg gacgcccaag caggaacatt tctgaatcag gtaattcctc
    12841 ggcaggcaca tctgtcgcac tgcgcgaaat attatgctgc tcagcaattt tcagcgcctg
    12901 ctcaatactg ttcatccggc ggtcgtagat ggctttcgcc acctcttcct gacgcttcac
    12961 ctgagctttc atctggatgg tgcgtgccgc ccatgcgcct ttcagctcat cattcagatg
    13021 gctggctgca cgctggctgg caaaagcaac atactgacgt aacaggttat tagcgtcagg
    13081 cgcggtttcg gcaataagct tcacgctgtc attgaccgcg cgagtaaagt ctccggggat
    13141 aaactggatg ttgttaatca tttcatccag caacgccgca tcggctttgc tgttgcccac
    13201 catccgctgt ttgtaatagt cggtttgcag ccagaactct ctgcgggtat cccacgaggc
    13261 cagttgcata acaaactctt tataggcttc gtccatgacc gatggttgat cggcagaagc
    13321 catgtttgaa cggacatcca ggttacgcaa aaattgctgc tgcgagtaat atccccccag
    13381 catattcacc gttggacgat cggtaatcgc cgtcgagctc cactcctgac gagcaaaaaa
    13441 agtatacgcc agcgcgatta acgcaaacgc cagccccatg ccaataatcc atagcttccc
    13501 agcccacaag gtacgaaaca acccacgaat atccagttca ttttcagcgt cttcggccgg
    13561 tttcccaggc attggttgtg tcatcacatc ctcatttatt tggttaaatt ggggctgcca
    13621 ccacgatttc tacgcagtct gcgttttacg cgcttaataa agcgagcaac tttccaggca
    13681 cgcttaatgc aatatccata gaggaagaat gctagcaaaa agagcaccag catgacccac
    13741 tccgggacaa aatgagaata ttctgccagc acgccaatgg aagcgagcag tgctgcggca
    13801 agggtaatca gcacaaacgc ctgacgggaa gtaaacccgg cacgcatgat caaatggtga
    13861 atatgctgac ggtcaggaga gaatgggctc atgcctttac gcaggcgacg gtacataatc
    13921 gccaccatat ccattagcgg aatggcgatt atccacaaag cggtaaccgg gctgatggga
    13981 tgggttttgc cctgggtcgt ttcgagcagg atccagataa cggtaaaacc aatcagcgta
    14041 ctgcccgcat cacccataaa gactttgtag cggcgaccca ggataccaag gttaagcatg
    14101 atgtatggca ggatggcggc gatcatcgca aagcaccaga ttgcgaggct ggtttgcccg
    14161 tcgaaccaca aaatcatacc gattgctgca aacgagacgc aggacaaccc gcccagcaag
    14221 ccatcaatgc catcaaccat gttgaacgca ttaatggccg cccagacggc aaatagcgtc
    14281 aggaagtaac caaacggtcc gagcaccatc tcccaggagc caaagatata acccaggcta
    14341 ctgagataaa gtttgccgaa caccatcata acaatgccaa cagcggcctg tatggtggca
    14401 cggattttta cgctgatatc aaaacgatca tccagcgcgc caatgaaaac aagcacaccg
    14461 gcacaagcga gatagagaga tgcatgcgga atatagtaat cgacaattcc gaacgtgaag
    14521 caaatccctg cgtaaaccga aatcccccca acgagaggta tcaatccctg gtgacgtttg
    14581 cggaagtttg gtttatccac taaaccgact ttttttgcca ccttacgggc aaaaaacaga
    14641 aacagtgtcg tgaataaaaa aatactgatg agatcagtac tcactgtcag taaattcaca
    14701 atgcatgctc tcagagaaaa ttattagcag aagtataacc acgaagacct ttattcagaa
    14761 gggaaagtcc gttccgaacc caccagccct gtgcaaaaat caaccatttg atatgcaaat
    14821 tgcataacat gccagcaaat tccaggaatt ttcctaacat cgccagcgcg acattaagat
    14881 tacagatata aaagcaaaac gccacgtaaa cacgtggcgt ttttggcata agacaaattt
    14941 atgagcgttt catcatttcg aagaaatcgt cattggtctt ggtcatggcc agtttattaa
    15001 tgaggaattc cattgcatcg atttcgccca tcgggtgaat gattttgcgc aggatccaca
    15061 ttttctgcag ttcttcctga gtcgtgagca gctcttcttt acgggtaccg gaacggttgt
    15121 agtcgatagc cgggaagacg cgtttttcag cgatcttacg agagaggtgc agttccatgt
    15181 tgcctgtacc tttaaactct tcgtaaataa cttcgtccat tttagaaccg gtatcgataa
    15241 gcgccgtcgc gataatggtc aggctgccgc cctcttccac gttacgcgcc gcaccaaaga
    15301 agcgtttcgg acgatgaagg gcattggcgt ccacaccacc ggtcaacact ttacctgacg
    15361 ccggaacaac agtgttgtaa gcgcgcgcca gacgagtgat ggagtcgagc agaatgataa
    15421 cgtctttctt gtgctcaacc aggcgtttgg ccttctcgat caccatttcc gcaacctgaa
    15481 cgtggcgaga tgcgggttcg tcaaaggtag aagcaacaac ttcacctttt accagacgct
    15541 gcatctcggt tacttcttcc ggacgttcgt cgatcagcag aaccatcagc acacaatccg
    15601 ggtggttgta agcaatgctc tgagcaatgt tctgcagcag catagtttta ccggctttcg
    15661 gcggtgccac aatcagacca cgctgaccac gaccgatagg tgatgccaga tccagtacgc
    15721 gagcagttaa atcttcagta gaaccgttac cacgttccat acgcagacga gagtttgcgt
    15781 gcagcggggt taagttctca aagaggattt tgttgcgggc gttttcaggt ttgtcgaagt
    15841 taacttcgtt aactttcagc agcgcaaaat agcgttcacc ttctttcggc gggcgaatct
    15901 taccagagat ggtatcacca gtgcggaggt tgaaacggcg gatttggcta ggggaaacgt
    15961 agatgtcatc aggaccggcg aggtaggagc tgtctgcgga acggaggaaa ccaaatccat
    16021 cctgcaatat ctccagtacg ccatcaccaa agatatcttc gccactcttt gcgtgctgct
    16081 tcaggatggc aaaaataatg tcctgcttac gcatgcgagc caggttttcc agccccatat
    16141 tttcgccgag agtgatcagc tcagaaaccg gcgtattctt taattcggta agattcatag
    16201 tggtgtgagt tcttaaactt ggggtaaatc tcgaacttaa tgttgtgaat ggtatggcag
    16261 gatcatccat gcctgtttac ggtcatcgac tcatgtcttt tcgctgcctg gtcacaggag
    16321 agtacgcaga actgaaacga caagacggat tgagtgacga gccagaaatc tgttcacttc
    16381 gcatttaaga taaaacggga agcgttggaa attacaagat tcaaacttaa taaggtatgt
    16441 ttaatacgaa gtcaacacta agttagcatg actcacgccg ggcgtccagt ttttagcgac
    16501 ggggcacccg aacatgaaat tcccttacgc caggttagcg tcgaggaact ctttcaactg
    16561 acctttagac agtgcgccca ctttggttgc cgccacttca ccgtttttga acagcagcag
    16621 agtcgggata ccacggatgc catatttcgg cgcagtgcca gggttttgat cgatgttcag
    16681 ttttgcaacg gtcagtttgc cctgatattc gtcagcgatt tcatccagaa tcggggcgat
    16741 cattttgcac ggaccgcacc actctgccca gaaatcgacg aggatcgccc cgtccgcttt
    16801 gagtacatcc gtgtcaaaac tgtcgtcagt caggtgaata attttatcgc tcatatataa
    16861 ctccacagga ataagcctgg cgtgttggtt tcgttgttgg tgtaacatta accaactaaa
    16921 ggttgacttt atttcaccgg atacgctttc gtaaagcaat agtaagctga tattctacca
    16981 cactatgagc aaaacacatt taacagaaca gaagttttcc gacttcgccc tgcatccgaa
    17041 ggttgtagaa gcccttgaaa aaaaagggtt tcataactgt acgcccattc aggcactggc
    17101 ccttccgctg acgctggcgg gtcgtgacgt agccgggcag gcgcaaaccg gtaccgggaa
    17161 aacgatggcg tttcttacgt caacgtttca ttatcttctc tctcatcctg cgattgccga
    17221 tcgcaaggtg aatcaaccgc gtgccttaat tatggcaccg acgcgtgaac ttgccgtgca
    17281 gatccacgcc gacgcggaac cgctggcgga agctaccggc ctgaagctgg gcctggctta
    17341 cggtggtgat ggctatgaca aacagctgaa agtgctggaa agcggcgttg acattctgat
    17401 tggcaccacg ggtcgtttaa ttgactacgc caagcagaac cacattaacc tcggtgccat
    17461 tcaggtagtg gtactggacg aagccgatcg catgtacgat ctaggcttta ttaaagatat
    17521 ccgctggctg ttccgccgta tgccgcctgc aaatcagcgc ctcaacatgc tgttctccgc
    17581 cacgctttcg taccgggtac gtgaactggc gttcgagcag atgaacaatg ccgaatatat
    17641 tgaagtggaa ccggaacaga aaacgggcca ccgtattaaa gaagagcttt tctacccttc
    17701 taacgaagaa aaaatgcgtt tgctgcaaac gctgatcgaa gaagagtggc cagacagagc
    17761 gattattttc gccaatacca aacaccgttg tgaagagatc tggggccacc tggcggcaga
    17821 tggtcatcgt gtcggtttgt tgacaggcga tgtcgcgcag aaaaaacgtc tgcgtattct
    17881 tgatgaattt acccgtggcg atctggatat tctggttgcc accgacgttg ccgcgcgtgg
    17941 tttgcatatt ccggcagtga cgcacgtctt taactacgat ttacccgatg actgtgaaga
    18001 ttacgttcac cgtattggtc gtacaggtcg cgcaggcgca agcggtcact ctatcagcct
    18061 ggcgtgtgaa gagtatgcat tgaatttgcc tgctattgag acctatattg gtcactcaat
    18121 tccggtaagc aaatacaatc cggacgcatt gatgaccgat ctgccaaaac cgctgcgcct
    18181 cacgcgcccg cgcacaggca atggtccgcg tcgtactggc gctccgcgta atcgtcgtcg
    18241 ttcaggttaa ataaaatgtc atactcaaaa tcattcaaac tgtatcaaga tggcaagaga
    18301 atgaatcccg atgagcttac tgtagtaagt gattcgggtg aaagaatgca gccaacacag
    18361 agacagattg aaggatgaag agtatgggtt ccacctcgtc gctgtatgca gccattgatc
    18421 tcggttcgaa tagttttcat atgctggttg tgcgcgaggt ggctggaagc atccagacgc
    18481 tgacgcgaat taaacgcaaa gtgcgtctgg ctgctggcct gaatagcgaa aatgacctct
    18541 ctaatgaagc aatggagcgc ggttggcaat gtctgcgcct gtttgctgaa cgtctgcaag
    18601 atatccctcc ctcgcaaatt cgcgttgtcg ctacggcgac attacgactt gccgtcaatg
    18661 cgggtgattt tattgccaaa gcgcaggaaa tcctcggttg tccggtacag gtgatcagcg
    18721 gtgaagagga agcgcgtctg atttatcagg gcgttgctca caccactggt ggtgccgatc
    18781 agcgcctggt ggtggatata ggcggtgcca gtactgaact ggtaaccggc acgggtgcac
    18841 aaaccacctc gttgttcagc ctgtcgatgg gctgcgtcac ctggctggaa cgctattttg
    18901 ccgatcgtaa tctggggcaa gaaaattttg atgctgcaga aaaagcggca cgcgaagtgt
    18961 tacgtccggt tgccgatgaa ttacggtatc acggctggaa agtgtgcgtt ggtgcttccg
    19021 gcaccgtgca ggcgttacag gaaatcatga tggcacaggg gatggatgaa cgcattaccc
    19081 tggaaaagtt gcagcaattg aaacagcgag ccattcattg cggtcggctg gaggaactgg
    19141 agattgacgg gctgacgctg gaacgtgcat tagtgttccc gagtggtctg gcgatcctga
    19201 tcgccatttt taccgaactg aatattcagt gtatgaccct ggcgggcggt gcgctgcgtg
    19261 aaggcctggt ctacggcatg ttgcatctgg ccgtcgagca ggatattcgc agccgtacac
    19321 tgcgtaatat tcaacgccgc tttatgatcg atattgatca ggcacagcgc gtagccaaag
    19381 ttgcggctaa cttcttcgat caggtggaaa acgaatggca tcttgaagca ataagccgcg
    19441 atttgctcat cagcgcctgt cagcttcatg aaatcggcct gagcgttgac ttcaaacaag
    19501 ctccgcaaca cgctgcttat ctggtgcgta acctggatct tcccggtttt acccccgcac
    19561 agaagaaact gctggcgacg ctactgctca accagactaa cccggtcgat ctctcatcgc
    19621 tgcatcagca aaatgccgta ccaccgcgcg tcgcagaaca actctgccgt ttactccgcc
    19681 tggctatcat ttttgccagc cgtcgccgtg acgatctcgt gccagagatg acattacagg
    19741 ctaaccatga actgttgacc ttgacgcttc cgcaaggttg gctaacccaa catccgctgg
    19801 gtaaagagat tattgctcag gaaaaccagt ggcagagcta tgtccactgg ccgctggaag
    19861 tgcattaatg agtaagtgcc ggatgcgatg ctgacgcatc ttctccggcc ttgattattt
    19921 ccctcgtttt gccgccatca tcgctttcag attcgccaga tggctttgcc ctttctgcat
    19981 ccgttcttcg gcactgacca ctttgcgctc ctgttcccaa atcagatcat cctgcggcag
    20041 ctccagcaaa aagcggctcg gctccgggcg caccagttcg ccgtactgac ggcgttcttt
    20101 acacagcgta aaggtcaatt ccttctgggc gcgggtaatg ccgacatagg ccagccgccg
    20161 ctcctcatcg atattatctt catcgatact gctctggtgc ggcaaaaacc cttcttccat
    20221 accgaccatg tagacataag gaaactccag cccttttgac gcgtggagag tcatcagttg
    20281 cacctgatcc agctcttctt cactctcacc acgctccatc atgtcgcgta aagtaaagcg
    20341 cgtcaccacc tgggtgagcg tcatcggctc atccagttcg ctgccttcca gcatctccgt
    20401 catccagcta aacagttggt tgacgttctt catgcgcatt tcggcagctt tcgtgctggg
    20461 cgatgtttcg tacagccagg attcataatc catgccgtgg atcagatcgc gcaccgcggc
    20521 aatcggctcc cgctccgcca gacgctggat ttctgccaac cagtgagtga agcgggtcaa
    20581 tgcttcataa ccacgtccgc taagcgtctg actcaggccc atatcaaagc tggcggtaaa
    20641 catgctttta ttgcgcgtca tcgcccactc acccagcttt ttcagcgtcg ccgggccaat
    20701 ctctcgcttc ggcgtgttaa cgatacgcag aaatgcgctg tcatcgtccg ggttagtcag
    20761 cacgcgcaga taagccagca agtccttgat ttcaggacga gagaaaaacg acgtaccacc
    20821 agaaattttg tacgggatgc ggttttgcat caggaatttt tcaaacaccc gcgactgata
    20881 gttaccgcga taaagaatag cgtaatcttt gtactgcgtt ttattgacga agtgatgcgc
    20941 aatcagttca ccagtgacgc gctcggcttc atgttcttcg ttattcgcgc ttaaaacttt
    21001 tagctctgtg ccgtaaccca gttcggagaa cagacgcttt tcaaagacgt gcgggttatt
    21061 ggcgatcagg atgttcgccg ctttcaaaat acgtccggaa gagcggtagt tctgctcaag
    21121 cttaatcacc ttcagcgccg gaaaatcctg gctcagcagc accaggtttt gcggacgtgc
    21181 gccgcgccag gagtagatcg actggtcatc gtcacccacc acggtaaagc gcgcgcggct
    21241 gcccaccagc agtttcacca gttcatactg gctggtgttg gtgtcctgat attcgtccac
    21301 cagcaggtag cgaattttgt tctgccagcg ctcgcggact tcttcattgc gttgcagaag
    21361 tagcgtcggc aataaaatca gatcatcgaa gtcgagcaca ttacaggctt tcaggtgtgc
    21421 atcatacagc ccataacaat gggcaaaaat acggtcccgc tcgccaatcg cactggctgc
    21481 cgcctgggac ggtgttttga gatcgttctt ccagttagag atggtcgaaa tcaactgttg
    21541 caggagaact ttgtcatctt caatcagccc ttcggtcaac tctttgagca acgccagctg
    21601 atcggtatcg tcaaacagcg aaaagttcgc cttcatcccc agcgccgcat actcacgctt
    21661 gatgatatcc agccccagcg tgtggaaggt tgagatcatc agcccatgcg cctctttgcg
    21721 acccagcgtc tgtccgacac gctctttcat ctcgcgcgcc gccttattgg taaaggtcac
    21781 cgccgcgata tgtcgcgcct gataaccgca cccgcggatc agatgggcga ttttattggt
    21841 gataacacga gttttaccgg aacccgcacc cgccagcacc aggcaggggc cggtaacgaa
    21901 ttcgacagct tgttgttggc cggggtttag acgcatagga aaatcgctca atgaaagtcg
    21961 ggaggggaaa ataacagcgt ggtagtatag ccagcctcat ccttaccact caaggcacga
    22021 tcatggcaaa aacagcagca gcactgcata tccttgtaaa agaagagaaa ctggctctgg
    22081 atcttctcgt gcagattaag aacggtgccg atttcggcaa gctggcgaag aaacactcca
    22141 tttgcccatc aggcaaacgc ggcggtgatt taggtgaatt ccgccagggt cagatggttc
    22201 cggcgttcga taaagttgtg ttctcttgtc cggtactgga gccaaccggc ccgctgcaca
    22261 cccagttcgg ttaccacatc attaaggtgc tgtaccgtaa ctaatagcaa ggccttctcc
    22321 gggagaaggc cttgaactct gaatcgctat taacccgcaa cagcaatacg tttcatatcc
    22381 gtcatatagc cgcgcagttt cttacctact tgctcaatcg catggctgcg aatcgcttca
    22441 tttacatcac gcagctgcgc gttatctacc gcaccttccg gaatagcttt acccaaatcg
    22501 cccggttgca gttctgccat aaacggtttc agcagcggca cacaagcgta agagaacaga
    22561 tagttaccgt actcggcagt atcagagata accacgttca tttcatacag acgcttacgg
    22621 acgatggtgt tggcaatcag cggcagttcg tgcagtgatt catagtaagc agattcttcg
    22681 atgatgccgg aatcaaccat ggtttcgaac gccagttcaa cgcccgcttt caccatcgcg
    22741 atcatcagta cgcctttatc gaagtactcc tgctcgccga ttttgccttc atactgcggc
    22801 gcggtttcga aggcggtttt gccggtctct tcacgccagg tcagcagttt cttatcatcg
    22861 ttggcccagt ccgccatcat accggaggag aattcgccgg agatgatgtc gtccatatgt
    22921 ttctggaaca gcggtgccat gatctctttc agctgttcag acaacgcgta agcacgcagt
    22981 ttcgccgggt tagagagacg gtccatcatc agtgtgatac cgccctgctt cagcgcttcg
    23041 gtgatggttt cccaaccgaa ctgaattagt ttttctgcgt atgccggatc ggtgccctct
    23101 tccaccagct tgtcgaagca cagcagcgag ccagcctgca acataccgca caggatggtt
    23161 tgctcaccca tcaggtcaga tttcacttcc gcaacgaagg acgattccag cacgcccgca
    23221 cggtgaccac cggttgctgc cgcccatgct ttagcgatcg ccatgccttc gcctttcgga
    23281 tcgttttccg ggtgaacggc aatcagcgtc ggtacgccga atccacgttt gtactcttca
    23341 cgcacttcgg tgccagggca tttcggcgca accatgacta cggtgatgtc tttacggatc
    23401 tgctcgccca cttcgacgat gttgaaaccg tgcgagtaac ccagcgccgc gccgtctttc
    23461 atcagtggct gtacggtgcg cactacatca gagtgctgct tgtccggcgt caggttaacc
    23521 accagatccg cctgtgggat cagttcttcg taagtaccca ctttaaaacc attttcggtc
    23581 gctttacgcc aggacgcgcg cttctcggca atcgcttctt tacgcagagc gtaggagata
    23641 tcgagaccag aatcacgcat gttcagaccc tggttcagac cttgtgcgcc acagccgacg
    23701 atgactactt ttttaccctg aaggtagctc gcgccatcgg cgaattcatc gcgccccata
    23761 aagcgacatt tgcccagctg tgccagctgc tgacgcagat tcagtgtatt gaagtagtta
    23821 gccatggtga ttcctcgtga tgttgtgctt cttattgttc ggttcgcgtt tgcgagattg
    23881 aattcactat atgacaggaa atttattgcg gaaattgata tattcacaac gtcacattgc
    23941 aatttctgca acgtcaacat tgagggctgc gccattggat ttacgcgatc tgaaaacctt
    24001 cctgcatctg gcggaaagcc gccattttgg ccgcagcgcg cgggcgatgc acgttagccc
    24061 gtccacgctc tcacggcaga ttcagcgcct ggaagaagat ctcggccagc cgctgtttgt
    24121 gcgcgataac cgcacggtga cgctgactga agcgggcgaa gaactgcgcg ttttcgccca
    24181 gcaaacgctg ttgcagtatc agcagctgcg ccacaccatc gaccagcaag ggccgttgct
    24241 ctatggcgaa ttacatatct tctgctcggt gaccgccgct tacagccatc tgccgccgat
    24301 tctggatcgc ttccgcgcgg aacacccgtc ggtggagatc aaactcacta ccggtgatgc
    24361 ggcagatgca atggaaaaag tcgtcacagg tgaagctgat ctggcgattg ccgggaagcc
    24421 agagactctg cccggcgcgg tggcgttttc gatgttggag aatctggcag tggtgttgat
    24481 tgcacccgca ttgccctgcc cggtgcgtaa tcaggtttct gtcgaaaagc cggactggtc
    24541 aacggtaccg tttatcatgg ccaatcaggg gccggtgcgc cgccgcattg agttgtggtt
    24601 ccgacgcaat aaaatcagta acccgatgat ttacgccacg gttggcgggc atgaagcgat
    24661 ggtatcgatg gtggcgctcg gctgtggcgt ggcattgttg ccggaagtgg tgctggaaaa
    24721 cagccccgaa ccggtgcgta accgcgtgat gattttagag cgcagcgatg agaaaacgcc
    24781 gtttgagctg ggcgtgtgtg cgcagaaaaa gcggctacat gagccgctaa ttgaggcatt
    24841 ctggaagatt ttgccgaacc attaacccgc caaaaagaac ctgaacgccg ggttattggt
    24901 ttcgtcgtgg caatcgtagc ccagctcatt cagccgggtt tcgaaatccg gttcatggtc
    24961 gccaagttcg aacgccgcca gtacgcgccc gtagtcggtg ccatggctgc gatagtggaa
    25021 caaagaaatg ttccagtgcg tacccagcgt gttgaggaag cgcagcagcg cgcccggtga
    25081 ttccgggaat tcgaagctgt agaggcgttc ctgcaacgga tgtgacggac gcccgccgac
    25141 catatagcgc acatgcaact tcgccatttc gtcgtcggag agatccacca cgctgtagcc
    25201 gccgtcgttg agcatctgca aaatttcttt gcgctcttcg aggccgcggc tcaggcgcac
    25261 gccgacaaag atgcaggcgt ttttggcatc ggcaaaacgg tagttgaact cggtgaccga
    25321 tcgcccgcca agcagttggc agaatttgag gaagctgcct ttttcttccg gaatggtcac
    25381 cgccaacaac gcttcacgct gttcgcccag ttcgcagcgt tccgagacgt agcgcaggcc
    25441 gtggaagttc acgttggcac cggaaagaat atgcgccagc cgttcgccgc gaatgttgtg
    25501 ctgggcgata tattttttca ttcccgccag cgccagcgcg ccagagggtt ccgccaccgc
    25561 acgcacatct tcgaataaat ccttcatcgc cgcacagatc gcatcgctat cgacggtgat
    25621 gatgtcgtcg agatactcct ggcataaacg gaaggtttcg tcaccgatgc gttttaccgc
    25681 gacaccttcg gcgaacagtc ccacgcgtgg cagatcaacc ggatgacccg catccagcgc
    25741 tgctttcagg caggcggagt cttccgcttc tacggcgatc actttgattt gcggcatcag
    25801 ttgtttgatc agcaccgcta cgcccgccgc cagaccgccg ccgccgactg gcacaaatac
    25861 gcggtcgaga tgggcgtcct gctggagcag ttccagcgcc agcgtgcctt gcccggcaat
    25921 caccatcgga tggtcgaacg gcggcaccca ggtgaacccc tgctgctgtg acagttcgat
    25981 cgctttagct ttcgcttcgt caaagttcgc gccatgaagc agcacttcgc cgccgaatcc
    26041 acgtaccgca tcgactttga tgtcggcggt ggcggttggc ataacgatca gggccttcac
    26101 gcctaaccgc gcagaagaaa acgcgacgcc ctgcgcgtgg ttacccgcag aagcagtgat
    26161 cacgccgtgc gctttttgtt cttccgtcag gcccgccatc atggcgtatg cgccgcgcag
    26221 cttaaagctg tgcactggct ggcgatcttc gcgcttcacc agaatgacgt tatcaagacg
    26281 cgacgacagt ttttccattt tttgtagcgg cgtaacctgc gccgcctcgt aaaccggcgc
    26341 gcgcagcact gctcttaaat attcggcacc ttccggagca ccggacaggg gttgcgagtc
    26401 agccattatt aaccccccag tttcgattta tcgcgcaccg cacctttgtc ggcgctggtt
    26461 gccaggctgg cgtaggcgca cagggcaaag gaaacctgac gttcacggtt tttcggcgtc
    26521 caggctttgt caccccgagc ttcctgcgct tcacgacgcg ccgccagttc ggcatcgctt
    26581 acctgtaact gaatgccacg gttcgggatg tcgatagcga tcaggtcacc atcttcaatc
    26641 agaccaatgc tgccgccgct tgccgcttcc ggtgagacgt ggccgatgga aagaccagag
    26701 gtgccgccag agaaacgacc gtcggtgatc agcgcacagg ctttaccgag acccattgat
    26761 ttcaggaagc tggttgggta gagcatttcc tgcatgccag ggccgccttt cgggccttca
    26821 tagcggatca ccaccacatc gcccgcgaca actttgccac cgaggatcgc ttctaccgcg
    26881 tcgtcctggc tttcgtacac tttcgccggg ccggtgaatt tgaggatgct gtcatcgacg
    26941 cccgcggttt tcacgatgca gccgttttca gcaaaattac cgtagagcac cgccaggccg
    27001 ccgtctttgc tgtaggcgtg ttccagcgag cggatacagc cgtttgcgcg atcgtcatcg
    27061 agagaatccc aacggcaatc ctgcgagaat gcctgtgtag tacgaatgcc cgcagggcct
    27121 gcgcggaaca tattttttac cgcgtcatcc tgggtcagca taacgtcgta ttgttccaga
    27181 gtttgcggca acgtcaggcc aagtacgttt ttcacatcac ggttcagtaa ccccgcgcga
    27241 tccagttcac cgagaatacc gataacacca ccagcacggt gaacatcttc catatggtat
    27301 ttttgggtgc tcggcgcaac tttgcataac tgcgggactt tacgcgaaag tttgtcgata
    27361 tcactcatgg tgaagtcgat ttccgcttcc tgcgccgccg ccagcaggtg aagtaccgtg
    27421 ttggtagagc cgcccatcgc gatatccagc gtcatggcat tttcaaacgc cgctttgcta
    27481 gcaatattgc gcggcagtgc actttcgtcg ttttgctcgt agtaacgttt ggtcaattca
    27541 acaatgcgtt taccggcatt gaggaacaac tgcttacggt ccgcgtgggt tgccagcagc
    27601 gagccgttgc ccggctgcga caggcccagc gcttcggtca ggcagttcat tgagttagcg
    27661 gtaaacatcc cggagcagga gccgcaggtc ggacacgcgg aacgttcaac ctgatcgctc
    27721 tgggagtcag aaactttcgg gtctgcgccc tggatcatcg catcaaccag atcgagtttg
    27781 attatctgat cggacagttt ggttttcccg gcctccatcg ggccgccgga aacaaagatc
    27841 accggaatat tcaggcgcag ggaagccatc agcatccccg gggtgatttt gtcgcagtta
    27901 gagatgcaga ccatggcgtc ggcgcagtgg gcgttgacca tatactcaac ggaatcagcg
    27961 atcagttcgc gagatggaag tgaataaagc atccccccgt ggcccatggc aatcccatca
    28021 tccaccgcaa tggtgttgaa ctctttggca acgccgccag ccgcttcaat ttgttcggcg
    28081 accagtttac cgagatcgcg cagatggacg tgacccggta caaattgggt gaacgagttc
    28141 acaaccgcga taatcggctt gccaaaatcg gcgtcggtca ttccggtggc gcgccacagc
    28201 gcacgagcac ccgccatatt acgaccatga gtggtggtgg cggaacggta cttaggcata
    28261 ctttatttac tcccagtgtc tgtctcgtaa atgggacggt gcgtgccgtc ccattttttg
    28321 tatttattga ttaacttgat ctaaccagcc ccatttatct tcggtttcgc cagtgaagag
    28381 gccgaagaag gcttgctgaa tgcgtttggt aaccgggcca caacggcctt cgccaatctg
    28441 aataccgtct acgctgcgca ctggcgtgat ttctgccgcc gtaccggaca taaagacttc
    28501 atccgccaag tacagggatt cgcgcgacag cacctgctca cgcacttcaa ttcccagctc
    28561 tttcgccagt ttgatgatgg catcacgggt aatacccggc agcgcggagg aggtgaacgg
    28621 tggggtgaac agcacaccat ctttcacttc aaacaggttt tcgcctgcgc cttcagagat
    28681 ataaccgttc acatccagcg cgataccttc ctgataaccg tggcggcgcg cttcgctacc
    28741 caccagcagg gaagagagat agttaccacc ggcttttgcc gcagttggga tggtgtttgg
    28801 tgctgcgcgg ttccaggagg aaaccatcgc atcgatcccc tgctccagcg cttctgcgcc
    28861 cagatacgct ccccacggga aagcggcgat aatcacatcg gttgagtatc ccgctggcgg
    28921 gtttactccc atgccaacat caccgacgaa gatcagcgga cggatatagg cgctggtgag
    28981 attgtttttg cggatcacgt cacgacaagc ttccatcagc tcatcaatgc tctgtgaaac
    29041 cgggaagcga tagattttgg cggagtcatg cagacgctgc atatgctcac gatggcggaa
    29101 tacaaccggt cctttgtgtg agtcgtagca acggatgcct tcaaaaaccg aggtgccata
    29161 gtgcagcgcg tgcgacatca catgcacctt cgcgtcttcc cagcgaacca tctccccatt
    29221 gaaccaaatg taatcagctt tcttcgtggt catttttata ttccttttgc gctcaggcgc
    29281 ggatttgttg tgatgtggtt gtgctctggc agatggcaac gtgtgcgacg tccaccagtt
    29341 tatttaactg actaaacagt aagtcgaccg accgtgggct ggcaacggtc aattcgatat
    29401 ttatattttg tgcatcgctg gcggcagcca tattcattga gcagacgtgg aaaccacgat
    29461 gacgcaccac gcgtaaaaca cgttctaagg tttccggatt gaagcgagcc gatacattga
    29521 cctgatgttg catcatgata atttctccaa catttctgaa ttactggcac ctggcggcac
    29581 cagcggccag acgttctcaa gttcgtcgat tgagacatga agcaggtatg gcccatcact
    29641 gttcagcatg gtgtcgagtg ccgcttcaac ctggtcttta cgggtgatgt gttggccagg
    29701 gatgccgaag gcgctggcta acatgaggaa atcggggtta tcggtaaggg tggtttcgct
    29761 gtatcgttcc tgaaaaaaca gttgctgcca ttgtcgaacc atccctaacc gttggttatc
    29821 gagtaagacg attttcaacg gtaactgctt gcgttttacg gtgcccagct cttgcacatt
    29881 catcatgaaa gagccgtcac cggagataca gacaacggta tcgttcggtc gcgcgacttg
    29941 tgcgccaact gccgccggta aaccaaaacc catggtacct aagccgctgg aggtgatgaa
    30001 attttccggg cgagtgtggg cgatgtgctg cgcagcccac atctggtgct gccccacatc
    30061 tgtggtcacg acgcaatccg caggtttacg atccgacagt tgttttaaca acaacggagc
    30121 gtagatagcg tcaccgggat ggtcgtaacg ccaggaatgt tcatcacgca gctgcgcgca
    30181 gtgttgctgc cagtcattga tatttaacgg ctgctgtaat gctggtaaca gagcatttaa
    30241 atcaccttgt aatgccacat gtgcctgacg cagcttgttc atttctgccg ggtcgatatc
    30301 catatggata acactggcgt gtggtgcgaa ggtgttcagt ttgccggtca cccggtcatc
    30361 aaaacgtgca cccacggcga tcagcaggtc acactcctgc accgcgaagt ttgccgcttt
    30421 ggttccgtgc atccccagca tgcccagata gtacggataa tctgcttcta ctgcgcccag
    30481 ccctttcagc gtacaggtgg caggcatttt tgtggcagcg agaaattcac gcaaagccgg
    30541 aactgcctgc gccatcccca cgccaccgcc aacgtacagc atcggttttt gcgcttttgc
    30601 cagcatctgg cgcgcttgct cgacttcggc atgtgggaaa gtcacttcgt tttcaacggt
    30661 ggtgaaccac ggttccagat cgccgctggc taactggata tcttttggga tatcgaccag
    30721 aaccggacca ggacgacctg agctggcaac gtcgaatgct tcagccatga tgcgcggcaa
    30781 ctcttccaga gactgcacca gaaagctgtg cttggtacag gctaacgaca atcccaggac
    30841 atccacttcc tgaaatgcgt cggtgccgat aaacggtgcg gacacctgac cagtgatggc
    30901 aacgacaggg atggaatcta acagtgcgtc cgcaagcccg gttatcaggt tggttgcgcc
    30961 cggaccagac gtggcgatac atacgccagt tttaccggta gcacgggcat aaccgatagc
    31021 cgccattgcc gcaccctgct catgtcggca cagcaagtgc tccacgccgc cgtcatacaa
    31081 tgcatcgtaa accggcataa ttgcgccacc cggataaccg aaaacggtgt tcacaccctg
    31141 tgcccgcaac gcatgtacca cccactgtgc gccattcata gttagttccc cgtcctgaat
    31201 cttgagaaac agaattttgt gctgttattc attgtctgct cctcggttat gtttttaagg
    31261 tcaaaaaaac ccccggacct ttcggtgcgg gggtcttagt tcgttaaggc ttgatctcta
    31321 agcctttcct cgtccaagtg cagccccgca cggtgggata ataatcacca ccacgctaat
    31381 cacgaccagg ctaatcactc gtagaagggc tgtcattttg tcttttcttg catcttgttc
    31441 gaaggaatgc ctaaagagtt accatagatt ttgcgaatag cacaagatat ttttgggcct
    31501 atttttatga tggctttaat aatttcaata gaaattgttg ttttttaaaa agaaaatagt
    31561 gagtgaaaaa attcaatttc agtataaatg ttgttccctt cctcgtagtt catcctttgt
    31621 ccgctgcatt tttgcgagca tcattccacc gccagaacaa ctcaacttca cttgcacaaa
    31681 taaacaggaa tcagccacta aaattcacga attagcgggc tacagcagcg atatcttatg
    31741 cacataatgg tgactcaagg agggtttatg tcactgtcaa ttgttcatac ccgcgcagcc
    31801 ctggaagtaa atgcgccccc aatcactgtt gaggtacata tcagtaaagg tctacccggc
    31861 ttaacgatgg tgggcttaac agaaacaacg gtgaaagaag ctcgcgatcg cgtgcgcagc
    31921 gccattatca atagcggata tgaatatccg gcgaaaaaaa tcaccatcaa ccttgctcca
    31981 gccgatctgc caaaagaagg gggacgatat gatttaccta tcgccattgc gttgctggcg
    32041 gcctcagaac agcttacagc caataagtta gatgaatatg aattagtcgg agaactagcg
    32101 cttacaggcg ctctgcgtgg cgttcccggt gcaatctcca gtgcaactga agctattaag
    32161 tcgggcagaa aaattatcgt cgcgaaagat aacgaagatg aagtggggct aattaacggt
    32221 gaaggatgcc tgatagccga tcatctgcag gctgtctgtg cgtttctgga aggtaagaac
    32281 actctcgaac gcccgaaacc aactgatgca gtatcccggg cgctacaaca tgatctcagt
    32341 gatgttgtcg gtcaggagca aggaaaacga ggactggaaa ttaccgccgc tggtgggcac
    32401 aaccttttac tgattgggcc gccgggaaca ggtaaaacaa tgctcgccag ccgtattaat
    32461 ggcctgttgc cagatttaag caatgaagag gcactggaga gcgctgcgat attaagtctg
    32521 gtaaatgctg aatcagtaca aaaacaatgg cggcagcgcc cgttccgctc acctcatcac
    32581 agtgcgtcgt taactgcgat ggtgggcggt ggtgcaattc cagggccagg tgaaatttcg
    32641 ttggcgcata acggcgtgct ttttcttgat gagctacctg aatttgaacg gcgtacactg
    32701 gatgccttgc gagagccgat tgaatccggg cagatccatc tttcacgcac acgagcaaaa
    32761 ataacctatc cagcccgttt ccagcttgtt gcggcgatga atcccagccc taccggacat
    32821 tatcagggaa accataaccg ctgcacgcca gaacagacat tacgttatct cactggagat
    32881 cccattacca ccccccggca ttttgagtaa aacggtagtg ccgggagaaa gcagcgccac
    32941 cgttaaacaa cgcgtaatgg ccgccagaga gcgccaattt aagcggcaga ataaactgaa
    33001 tgcctggctg gatagtccgg aaatacgcca attctgcaaa cttgagagcg aagatgcgat
    33061 gtggttggaa gaaacgctga tccatctggg gttatcgatt cgtgcctggc agcggttact
    33121 gaaagttgca cgaaccattg ctgatattga tcagtctgac attatcacac gtcagcattt
    33181 gcaggaggca gttagctatc gtgcgattga ccgtttgctc atccatctgc aaaaactact
    33241 gacataaaaa aagggcattt cgcccttttt attaatcgtc agaatcggtg tagtcttcag
    33301 caccttcaac ctgcggttta ccgccggaaa gggtgtgaaa acgttttgga cgcttaatac
    33361 gcgtcatata cttggaccac acgcgttctg cttctgtcac tggctcacgt tcgccacggc
    33421 atactgctac gaagagtttc tcttcctcgg taaccggctc gcgtttgcca agatccaact
    33481 cattgaaggc ataaccatga cgctcaagca gttgtgcctc tttgatggtg aaatcaccat
    33541 gacgagagaa tccacgtgga taatgtttat tgtcgaaata tcgattagtc gtcgtaaagc
    33601 tttccgccat cctgcacgct cctaattctt tgaccgagct agttatggcg cggagtatta
    33661 gttacgcttg acagagtgta aaacaaaaca tttaaatcat aacgacaaat aattttgcgg
    33721 agagcactgt ggatacggaa ttgttaaaaa ctttcctgga agttagccga acgcgtcact
    33781 ttggtcgagc ggctgaatcg ctctatctga cccagtcagc agtgagcttt cgaatcagac
    33841 aactggaaaa tcaactgggt gtgaaccttt tcacccgcca cagaaacaat atccgtttaa
    33901 ccgctgccgg tgaaaaacta ctgccttatg cagaaacgct catgagcacc tggcaggccg
    33961 cccgcaagga ggtggcgcat acctcacgac ataacgagtt ttctatcggt gccagcgcct
    34021 cgttgtggga atgtatgctt aatcagtggt tgggacgctt gtatcaaaat caggatgccc
    34081 atacaggctt acagttcgaa gcgcgaattg cccaacggca gtctctggta aaacagctgc
    34141 atgaacgcca gcttgatctt cttattacca ctgaagcgcc caaaatggac gaatttagta
    34201 gtcagttgct ggggtatttc actttagcgc tttataccag tgccccttct aaactaaagg
    34261 gagatcttaa ctatctgcga ctggagtggg ggccagattt tcaacagcat gaggcaggtt
    34321 tgatcggtgc tgacgaagtt cccattctga caaccagttc tgctgaactg gcacagcaac
    34381 agattgcgat gcttaatggt tgcacctggc tacccgtcag ctgggcgcgt aaaaaaggcg
    34441 gcctgcatac cgttgtcgat agcacaacac tttcacggcc gctttatgcc atatggctgc
    34501 aaaatagcga taaaaatgcg ttgattcgcg atcttttgaa aattaacgtg ctggatgaag
    34561 tgtattaatc tgaatggctg gcaaggatgc cggtagaagg atttacttcg gagaggatta
    34621 tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggcagg ggcggagaga
    34681 ctcgaactcc caacacccgg ttttggagac cggtgctcta ccaattgaac tacgccccta
    34741 attag
//
LOCUS       DAJTSA010000035        34446 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000035 DAJTSA010000000
VERSION     DAJTSA010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.035"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..333)
                     /locus_tag="OO369_001829"
     CDS             complement(<1..333)
                     /locus_tag="OO369_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5982128.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQK"
     gene            703..1653
                     /gene="corA"
                     /locus_tag="OO369_001830"
     CDS             703..1653
                     /gene="corA"
                     /locus_tag="OO369_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR5982129.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1694..2074)
                     /locus_tag="OO369_001831"
     CDS             complement(1694..2074)
                     /locus_tag="OO369_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCR5982130.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2088..2504)
                     /locus_tag="OO369_001832"
     CDS             complement(2088..2504)
                     /locus_tag="OO369_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5982131.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2563..3453)
                     /gene="rarD"
                     /locus_tag="OO369_001833"
     CDS             complement(2563..3453)
                     /gene="rarD"
                     /locus_tag="OO369_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR5982132.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3505..3972)
                     /locus_tag="OO369_001834"
     CDS             complement(3505..3972)
                     /locus_tag="OO369_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR5982133.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4137..5006
                     /gene="pldA"
                     /locus_tag="OO369_001835"
     CDS             4137..5006
                     /gene="pldA"
                     /locus_tag="OO369_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR5982134.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5139..6968
                     /gene="recQ"
                     /locus_tag="OO369_001836"
     CDS             5139..6968
                     /gene="recQ"
                     /locus_tag="OO369_001836"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR5982135.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7032..7652
                     /gene="rhtC"
                     /locus_tag="OO369_001837"
     CDS             7032..7652
                     /gene="rhtC"
                     /locus_tag="OO369_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR5982136.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7714..8334)
                     /gene="rhtB"
                     /locus_tag="OO369_001838"
     CDS             complement(7714..8334)
                     /gene="rhtB"
                     /locus_tag="OO369_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR5982137.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8445..9467
                     /gene="pldB"
                     /locus_tag="OO369_001839"
     CDS             8445..9467
                     /gene="pldB"
                     /locus_tag="OO369_001839"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR5982138.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9475..10275
                     /gene="yigL"
                     /locus_tag="OO369_001840"
     CDS             9475..10275
                     /gene="yigL"
                     /locus_tag="OO369_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR5982139.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10351..11250
                     /gene="bioP"
                     /locus_tag="OO369_001841"
     CDS             10351..11250
                     /gene="bioP"
                     /locus_tag="OO369_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR5982140.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11138..12091)
                     /gene="metR"
                     /locus_tag="OO369_001842"
     CDS             complement(11138..12091)
                     /gene="metR"
                     /locus_tag="OO369_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR5982141.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12327..14588
                     /gene="metE"
                     /locus_tag="OO369_001843"
     CDS             12327..14588
                     /gene="metE"
                     /locus_tag="OO369_001843"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR5982142.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14627..15442)
                     /locus_tag="OO369_001844"
     CDS             complement(14627..15442)
                     /locus_tag="OO369_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5982143.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15704..16465
                     /gene="udp"
                     /locus_tag="OO369_001845"
     CDS             15704..16465
                     /gene="udp"
                     /locus_tag="OO369_001845"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR5982144.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16606..18033
                     /gene="rmuC"
                     /locus_tag="OO369_001846"
     CDS             16606..18033
                     /gene="rmuC"
                     /locus_tag="OO369_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR5982145.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18128..18883
                     /gene="ubiE"
                     /locus_tag="OO369_001847"
     CDS             18128..18883
                     /gene="ubiE"
                     /locus_tag="OO369_001847"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR5982146.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18897..19502
                     /gene="ubiJ"
                     /locus_tag="OO369_001848"
     CDS             18897..19502
                     /gene="ubiJ"
                     /locus_tag="OO369_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR5982147.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19499..21139
                     /gene="ubiB"
                     /locus_tag="OO369_001849"
     CDS             19499..21139
                     /gene="ubiB"
                     /locus_tag="OO369_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR5982148.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21218..21487
                     /gene="tatA"
                     /locus_tag="OO369_001850"
     CDS             21218..21487
                     /gene="tatA"
                     /locus_tag="OO369_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR5982149.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21491..22006
                     /gene="tatB"
                     /locus_tag="OO369_001851"
     CDS             21491..22006
                     /gene="tatB"
                     /locus_tag="OO369_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR5982150.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22009..22785
                     /gene="tatC"
                     /locus_tag="OO369_001852"
     CDS             22009..22785
                     /gene="tatC"
                     /locus_tag="OO369_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR5982151.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22827..23609
                     /gene="tatD"
                     /locus_tag="OO369_001853"
     CDS             22827..23609
                     /gene="tatD"
                     /locus_tag="OO369_001853"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR5982152.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23606..24094)
                     /gene="rfaH"
                     /locus_tag="OO369_001854"
     CDS             complement(23606..24094)
                     /gene="rfaH"
                     /locus_tag="OO369_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR5982153.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24261..25754
                     /gene="ubiD"
                     /locus_tag="OO369_001855"
     CDS             24261..25754
                     /gene="ubiD"
                     /locus_tag="OO369_001855"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR5982154.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25800..26501
                     /gene="fre"
                     /locus_tag="OO369_001856"
     CDS             25800..26501
                     /gene="fre"
                     /locus_tag="OO369_001856"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR5982155.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26693..27856)
                     /gene="fadA"
                     /locus_tag="OO369_001857"
     CDS             complement(26693..27856)
                     /gene="fadA"
                     /locus_tag="OO369_001857"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR5982156.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27866..30055)
                     /gene="fadB"
                     /locus_tag="OO369_001858"
     CDS             complement(27866..30055)
                     /gene="fadB"
                     /locus_tag="OO369_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR5982157.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30245..31576
                     /gene="pepQ"
                     /locus_tag="OO369_001859"
     CDS             30245..31576
                     /gene="pepQ"
                     /locus_tag="OO369_001859"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR5982158.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31576..32190
                     /locus_tag="OO369_001860"
     CDS             31576..32190
                     /locus_tag="OO369_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR5982159.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32229..33680
                     /gene="trkH"
                     /locus_tag="OO369_001861"
     CDS             32229..33680
                     /gene="trkH"
                     /locus_tag="OO369_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR5982160.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNAYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33692..34237
                     /gene="hemG"
                     /locus_tag="OO369_001862"
     CDS             33692..34237
                     /gene="hemG"
                     /locus_tag="OO369_001862"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR5982161.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8108 a         8700 c         9302 g         8336 t
ORIGIN      
        1 cttctgctga ccgttttcga tgtacaaacc gagcggcgca tagctttcat catagatgcc
       61 gccgttcatc gccatctgca cctgaccctg actattaata tccgccagca gagcatgtaa
      121 cgttccccac gcttcgccat tggctttttg ccagtacatt ttcacccgct ctgtttgagg
      181 attaacggta tacgcctgta cggtcagcgt cggatctgag agtgcgcaat catcagcggc
      241 aacagcaaac aagggaagta aggtgagggt gagaaaaatc cgtttgagat tcaaggtgat
      301 cattccttta ccaatgagta gccgatgcgc cattataggt cctagatgtg ggattttttt
      361 atcctgttag cgaccttgac gagtaccaaa aagcgcgaag ttcaactatt gttctgtggt
      421 gttctgttgc gtgttgacgg caaaattttg ctggcgtaac atgcgcgcac gatcactcta
      481 agaggacatt cgccttggac acacccagta gatactggct cactatcctg tcatccagga
      541 tcaactccta aggctatccc tttttgctga tagccttagc ggttgtcagc gacctcaatt
      601 tttcccgtcg cgctgagtca ggctgtttaa tggtctgaaa cccaatttgt ttctgtgtgc
      661 ccaccgaact gtccgatatt ttaagcattg ggagtcccgg tcatgctgag cgcatttcaa
      721 ctggaaaata accgactgac ccggctggaa gtcgaagagt cacaacccct tgtaaatgca
      781 gtatggattg atcttgtcga accggacgac gacgagcgac tgcgcgtaca atctgaactt
      841 ggccagagcc tggcaacccg cccggaactg gaagacatcg aagcatcggc acgtttcttt
      901 gaagacgacg acggcctgca tattcactcc ttcttcttct ttgaagatgc ggaagatcac
      961 gccggtaact ccactgtggc atttaccatc cgtgatggtc gtctgtttac tctgcgtgag
     1021 cgtgaactgc ccgcttttcg tctgtatcgt atgcgtgccc gtagccagtc gatggtagac
     1081 ggtaacgcct acgagttgct gctggatctg ttcgaaacca aaatcgaaca gttggcagat
     1141 gaaattgaaa atatctatag cgacctggag cagttgagcc gggtgattat ggaagggcat
     1201 cagggcgatg agtacgacga ggcgctctcc acgctggcgg aactggaaga tatcggctgg
     1261 aaagttcgcc tgtgtctgat ggatacccag cgcgctctca acttcctggt gcgtaaagcg
     1321 cgtttaccgg gtgggcaact ggagcaggcg cgtgaaatcc tgcgcgatat cgaatccctg
     1381 ctgccgcata acgaatccct gttccagaag gtgaacttcc tgatgcaggc ggcaatgggt
     1441 tttatcaaca tcgagcagaa ccgcatcatc aaaattttct cggtggtatc cgtcgtgttc
     1501 ctgccgccaa cgctggttgc ttccagctac gggatgaact ttgagtttat gccagaactg
     1561 aagtggagtt tcggctaccc tggcgcgatt atctttatga tcctcgcggg cctggcaccg
     1621 tatctgtact ttaagcggaa gaactggttg taaaaagaag gcggtggctt agcctggcta
     1681 agccacctgt tattcaaagg ctccaggtat ttaacccttt tacctctttc tcataaaacc
     1741 atttgttcgt gttaacagca acataggctg ctacggcaat tcccagaatg ttaacgccaa
     1801 ttaatgcacc aacaaataga gatagaatgc caagtaatag aactataatt gcttttttcc
     1861 ataagcccag aacaaataaa tagatccagg aacagaagaa agcaatgaaa ttcatttgaa
     1921 tagttaatcg ttgtcttact tttaatgctt taaatgctgc tttatattct ggtgttgctc
     1981 cccagaatcc aggaaagcca tgttgatcat aaaaattaaa tcggtatttc catttttcac
     2041 ttaatgaatc atcgttcata tattccttac tcataaatac tccataacta ttgttttgat
     2101 gaatcagtag gtgcaagcat tagcatactg aaagtggaaa aataacaaat caaaaaaatc
     2161 atcgaaccat tgcctgaaca ggcaaaatct tcggctatca ttgtgatgat agagatgata
     2221 tatactgcta atgtaccaaa aacataaatt tttatatata tgaaaccact atcacggagt
     2281 cgttggcaat tcatgttgat gacgagataa tggagtgcga tggtagagac tataacaaga
     2341 aaacctgctt ccccatcgtt aaaaaagata ataagaaagg caaaaatgaa atttattaaa
     2401 ataaataaaa atatatatcg gtgtctggaa atcttaccgt tagatgttgg gataaacata
     2461 cgtaacataa attttacatc cttgtgtgag tctcctgtca gcatggcaat atgcccactc
     2521 tcatacaaga gtcggcatat tttttcagaa tatatttatt ttttatttgg acgttctacg
     2581 ctgcgtataa atcgcgtcca tcacaaaaat tgccagcgcc acccaaataa aggtgaaagt
     2641 caccatctta tcggcacccg gtttttcacc ataaaacgtc acagccagca ggaacatcag
     2701 cgtcgggcca atgtactgaa aaaagcctaa cgttgagaga cgcaggcgcg tggcagcggc
     2761 agtaaaacac aacagcggta cggtagtgac aataccggcg gcgatcagca gtaaattcag
     2821 cgacatcggg ttttgcccca tatggctggt tgaactgtcg gcaatagcaa acaggtaaat
     2881 cgccgccacg ggcagcagcc acatggtttc aattaacatg ccggtttgtg cttctaccgc
     2941 aatcttcttg cgcaccaggc cgtagaaggc aaaactaaat gccagcccca gcgcgataat
     3001 aggtagcgaa ccaaaagtcc acagctggac caacacgcca catatcgcca gaatcaccgc
     3061 cagccattgc atccggcgga atcgctcgcc gaggaaaatc atccccagca caatgttcac
     3121 cagcgggtta ataaagtaac caaggctcgc ttccagcata tggtgattgt tcaccgccca
     3181 gataaacagt agccagttgc caccaatcag tacggcagag actgccagca taaaaatttt
     3241 ctgtggcgtc tgaatcagcg tttttaaata ggaccactgg cggcaaatgc tcatcagcac
     3301 caccataaag aaaaacgacc agatcacgcg atgcgtcagg atctcatcgg cgggcacgta
     3361 gtaaatcagc ttgaagtatg ctggcgcaat accccaaata aaataagcgg caagagcgag
     3421 taatacgccc tgccgcgttt gttttgcatc catcgggaat actcattttt aattggtaac
     3481 agcagtttac ctgcttttat gtcttcaacc taccatatag gtggcggtgg cactggcaat
     3541 ataaagctgt tcttcattgt gtaattcaac gcgggcgacg gcgactttat tgcctgcacg
     3601 caacaggcta ctggtagcag taaaacgctc gcccctgcct gggcgcagat aatcaacgcg
     3661 aagatcaatg gtccccatcc gcgatagccg ctggcgtagt tcatcttcac tgatggtttc
     3721 gtggcgggtt aaggtacttc ccacgcacac cagaccggcg gcgacatcca gcgccgacgc
     3781 aatgactccg ccgtgcaaaa tgctttgcgc ccagttaccc accatcatcg gctgattttt
     3841 aaaggccagc tgtgcgaact ctttttcgta acgctccagt tccatcccca atgcgcggtt
     3901 aaatggcatg tgataaacaa acatctcacc cactaatttc agggcttgtt cagcggtcag
     3961 tacggcagac atatcatcct tacacttcat tggttaatga aatgttgatt ttatgcttct
     4021 ttgttgttgg tttctacttt aggaagggat aactaacggc aatggagtta agtatgtaaa
     4081 atagcccgca gaaaaacatt caccttatca ataattcgtt acggagaaca cgaccgatgc
     4141 ggactctgca gggctggttg ttgccggtgt ttatgttgcc tatggcagta tatgcacaag
     4201 aggcaacggt gaaagaggtg catgacgcgc cagcggtgcg tggcagtatt atcgccaata
     4261 tgctgcagga gcatgacaat ccgttcacgc tctatcctta tgacaccaac tacctcatct
     4321 acacccaaac cagcgatctg aataaagaag cgattgccag ttacgactgg gcggaaaatg
     4381 cgcgtaagga tgaagtaaag tttcagttga gcctggcatt tccgctgtgg cgtgggattt
     4441 taggcccaaa ctcggtgttg ggtgcgtctt atacgcaaaa atcctggtgg caactgtcca
     4501 atagcgaaga gtcttcaccg tttcgtgaaa ccaactacga accgcaattg ttcctcggtt
     4561 ttgccaccga ttaccgtttt gcaggttgga cgctgcgcga tgtggagatg gggtataacc
     4621 acgactctaa cgggcgttca gacccgacct cccgcagctg gaaccgcctt tatactcgcc
     4681 tgatggcaga aaacggtaac tggctggtag aagtgaagcc gtggtatgtg gtgggtaata
     4741 ctgacgataa cccggatatc accaaatata tgggttacta ccagcttaaa atcggctatc
     4801 acctcggtga tgcggtgctc agtgcgaaag gacagtacaa ctggaacacc ggctacggcg
     4861 gcgcggagtt aggcttaagt tacccgatca ccaaacatgt gcgcctttat actcaggttt
     4921 acagcggcta tggcgaatcg ctcatcgact ataacttcaa ccagacccgt gtcggtgtag
     4981 gggttatgct aaacgacttg ttttgatgaa cggttgagtg gttggcaaat ctggaatcca
     5041 gcatccagga ttaccctctc agagactaaa agcattgcag tttctcgcgc aggcgctgaa
     5101 aatagcgcct gtttttattt caggcaatcg gggtgaatgt ggcgcaggcg gaagtgttga
     5161 atctggagtc cggagctaaa caggttttac aagaaacctt tggctaccaa cagtttcgcc
     5221 ccggccagga agaaattatc gacactgtgc tttccggtcg cgattgcctg gttgttatgc
     5281 ccactggtgg cggaaaatcc ctttgctatc aaattcctgc cttattgcta aacggcctta
     5341 ccgtggttgt ttcaccgctg atttcgttga tgaaagatca ggtggatcaa ctacaggcca
     5401 acggcgtggc ggcggcgtgc cttaactcga cgcaaacccg cgaacagcaa cttgaagtga
     5461 tgacaggctg ccgcaccggg caaattcgct tactgtatat cgccccggaa cgcctgatgc
     5521 tggataactt tcttgagcat ctggcgcact ggaatccggt gttattagcc gtcgatgaag
     5581 cgcactgtat ctcccaatgg ggtcacgatt tccgcccgga atatgccgcg ctcggtcagt
     5641 tgcgccagcg gttcccgacg ctgccgttta tggcgctgac cgccacagcc gacgacacca
     5701 cgcgccagga tatcgtgcgc ctgctggggc tgaacgatcc gctgattcaa atcagcagtt
     5761 ttgaccgtcc gaatattcgc tacatgctga tggagaaatt caaaccgctc gatcagttga
     5821 tgcgctacgt gcaggaacag cgcggtaagt ctggcattat ctactgcaac agccgggcga
     5881 aagtagaaga caccgctgcg cgcctgcaaa gcaggggtat tagcgcggcg gcctatcatg
     5941 ccgggctgga aaataatgtc cgcgccgacg tgcaggagaa attccagcgc gatgatctgc
     6001 aaattgtggt ggcgacggtg gcgttcggca tgggcatcaa taaaccaaac gttcgcttcg
     6061 tggtccactt tgatattccg cgcaatatcg aatcctatta tcaggaaacc ggtcgcgccg
     6121 ggcgtgatgg cctgcctgcg gaagcgatgc tgttttacga tccggctgat atggcgtggc
     6181 tgcgccgttg tctggaagag aagccgcagg ggcagttgca ggatatcgag cgccacaaac
     6241 tcaatgcgat gggcgcgttt gccgaagcgc aaacttgccg tcgtctggta ttgctgaact
     6301 attttggcga agggcgtcag gagccgtgcg ggaactgcga tatctgcctc gatccgccga
     6361 aacagtacga cggttcaacc gatgctcaga ttgccctttc caccattggt cgtgtgaatc
     6421 agcggtttgg gatgggttat gtggtggaag tgattcgtgg tgctaataac cagcgtatcc
     6481 gcgactatgg tcatgacaaa ctgaaagtct atggtatggg ccgtgataaa agccatgaac
     6541 attgggtgag cgtgatccgc cagctgattc atctcggcct ggtgacgcaa aatattgccc
     6601 agcattctgc cctacaactg acagaggccg cgcgcccggt gctgcgcggc gaatcctctt
     6661 tgcaacttgc cgtgccgcgt atcgtggcgc tcaaaccgaa agcgatgcag aaatcgttcg
     6721 gcggcaacta tgatcgcaaa ctgttcgcca aattacgcaa actgcgtaaa tcgattgccg
     6781 atgaaagtaa tgtcccgccg tacgtggtgt ttaacgacgc aaccttgatt gagatggctg
     6841 aacagatgcc gatcaccgcc agcgaaatgc tcagcgttaa cggcgttggg atgcgcaagc
     6901 tggaacgctt tggtaaaccg tttatggcgc tgattcgtgc gcatgtcgat ggcgacgacg
     6961 aagagtagtc agcagcataa aaaagtgcca gtatgaagac tccgtaaacg tttcccccgc
     7021 gagtcaaatg tatgttgatg ttatttctca ccgtcgccat ggtgcacatt gtggcgctta
     7081 tgagccccgg tcccgatttc ttttttgtct ctcagaccgc tgtcagtcgt tcccgtaaag
     7141 aagcgatgat gggcgtgctg ggcattacct gcggcgtaat ggtttgggct gggattgcgt
     7201 tgcttggcct gcatttgatt atcgaaaaaa tggcctggct gcatacgctg attatggtag
     7261 gcggtggcct gtatctctgc tggatgggtt accagatgct acgcggtgca ctgaaaaaag
     7321 aggctgtatc tgcgcctgca ccacaggttg agctggcgaa aagcgggcgc agtttcctga
     7381 aaggcttact gaccaatctc gccaatccga aagcgattat ctattttggt tcggtattct
     7441 cattgtttgt cggcgataac gttggcacta ccgagcgctg gggcattttc gcgttgatta
     7501 ttgtcgaaac actggcgtgg tttaccgtcg ttgccagtct gtttgccctg ccgcaaatgc
     7561 gccgtggtta tcaacgtctg gcgaagtgga ttgatggttt tgccggggcg ttatttgccg
     7621 gatttggcat tcatttgatt atttcgcggt gatgccagac gcgtcttcag agtaagtcgg
     7681 ataaggcgtt tacgccgcat ccgacattat ttttcacgca tgcctcgccg atgctaacag
     7741 cgctcccacc agcataaaca acgagccgaa aatcttattc agcgccttca tctgctttgg
     7801 tcctttaatc catagagcaa tcctttgagc aagggtggcg taaccgatca tcacaataat
     7861 atcgaccaca atagtggtga cgccgagcac gatatactgc atcagttgcg gctgttgcgg
     7921 catgatgaat tgcggaaata gcgccgccag aaacacaata cttttgggat tggtgagatt
     7981 cacaaaaact gcgcgctgga acaaatgtcg acgcgattga gtagaggcca gcgatttaag
     8041 atcaattgcg ccagcggcgc gccactgctg gattcccagc caaatcaagt aagccgcgcc
     8101 tgcccacttt aacacttcaa acgcaatcac tgagcgggaa aatagcgtcc ccaaccccac
     8161 gccaaccagc acaatatgaa tcgccagtcc ggtctgaagc ccagcaatag acgccaccgc
     8221 gccgcgataa ccgtggttga gcgaggtggt catagtgttg attgcaccag agcctggcga
     8281 caggcttaaa atgatcgatg tcagcaggta ggcaaaccac cattctaagg tcatgatgaa
     8341 ctcccggtgt gtctattttt gtgccacaat acgctactgt cgcagcgttg tgtcaggcac
     8401 gcttaaaaaa acgattttac gtggtttaag aggcagatta cccgatgttt cagcagcaaa
     8461 aagactggga aacaagagaa aacgcgtttg ctgcttttac catgggaccg ctgactgatt
     8521 tctggcgtca gcgtgatgaa gcagagttta ctggtgtgga tgacattccg gtgcgctttg
     8581 tccgttttcg cgcacagcac catgaccggg tggtagtcat ctgcccgggg cgtattgaga
     8641 gctacgtaaa atatgcggaa ctggcctacg acctgttcca tttggggttt gatgtcttaa
     8701 tcatcgacca tcgcgggcag ggacgttccg gtcgcctgtt agccgatccg catctcgggc
     8761 atgttaatcg ctttaatgat tatgttgatg atctggcggc attctggcag caggaggttc
     8821 agcccggtcc gtggcgtaaa cgctatatac tggcacattc gatgggcggt gcgatctcca
     8881 cattatttct gcaacgccat ccaggtgtat gtgacgccat tgcgctaact gcgccaatgt
     8941 ttgggatcgt gattcgtatg ccgtcattta tggcacggca gatcctcaac tgggccgaag
     9001 cgcatccacg tttccgtgat ggctatgcaa taggcaccgg gcgctggcgc gcgttgccgt
     9061 ttgctatcaa cgtactgacc cacagcagac agcgatatcg acgtaactta cgcttctatg
     9121 ctgatgaccc aacgattcgc gtcggtgggc cgacctacca ttgggtacgc gaaagtattc
     9181 tggctggcga acaggtgtta gccggtgcgg gtgatgacgc cacgcaaacg cttctcttac
     9241 aagctgaaga ggaacgcgtg gtggataacc gcatgcacga ccgtttttgt gaactccgca
     9301 ccgccgcggg ccatcctgtc gaaggaggac ggccgttggt aataaaaggt gcttaccatg
     9361 agatcctttt tgaaaaggac gcaatgcgct cagtcgcgct ccacgccatc gttgattttt
     9421 tcaacaggca taactcaccc agcggaaacc gctctacaga ggtttaaatt tcttatgtac
     9481 caggttgttg cgtctgattt agatggcacg ttactttctc ccgaccatac gttatcccct
     9541 tacgccaaag aaactctgaa gctgctcacc gcgcgcggca tcaactttgt gtttgcgacc
     9601 ggtcgtcacc acgttgatgt ggggcaaatt cgcgataatc tggagattaa gtcttacatg
     9661 attacctcca atggtgcgcg cgttcacgat ctggatggca atctgatttt tgctcataac
     9721 ctggatcgcg acattgccag cgatctgttt ggcgtagtca acgacaatcc ggacatcatt
     9781 actaacgttt atcgcgacga cgaatggttt atgaatcgcc atcgcccgga agagatgcgc
     9841 ttttttaaag aagcggtgtt caaatatgcg ctgtatgagc cgggattact ggagccggaa
     9901 ggcatcagca aagtgttttt cacctgcgat tcccatgaac aactgctgcc gctggagcag
     9961 gcgattaacg ctcgctgggg cgatcgcgtc aacgtcagtt tctctacctt aacctgtctg
    10021 gaagtgatgg cgggcggcgt gtcgaaaggc catgcgctgg aagcagtggc gaagaaactg
    10081 ggctacagcc tgaaggattg tattgcgttt ggtgacggga tgaacgacgc cgaaatgctg
    10141 tcgatggcgg ggaaaggctg cattatgggc agtgcgcacc agcgtctgaa agatctgcat
    10201 ccggagctgg aagttattgg tactaatgcc gacgacgcgg tgccgcatta tctgcgtaaa
    10261 ctctatttat cgtaatcgtt ctttatttgg tcagttgtca acctgatact tcgctacaat
    10321 ggatactcgt taatcaaaga gttttccatt gtggcgttac ttatcatcac cacgattctg
    10381 tgggccttct cctttagctt ttatggcgag taccttgcgg ggcacgtcga tagctatttt
    10441 gcggtgctgg tgcgcgttgg cttggcggca ctcgtttttc tgccgtttct gcgtacccgt
    10501 agcaatagcc tgaaaacggt cggcctgtat atgctggtgg gcgcgatgca gctcggcgtg
    10561 atgtatatgc tgagtttccg cgcttatctc tacctgacgg tctccgaatt gttgctgttc
    10621 accgtactga cgccgctcta catcacgctg atttatgaca tcatgagtaa gcgccgtctg
    10681 cgctggggct atgcttttag cgccttgctg gcggtgattg gtgccgggat tattcgctat
    10741 gatcaggtca ccgaccattt ctggactggc ttgttgctgg tgcaactctc caatatcact
    10801 tttgccatcg gcatggtggg ttacaaacgc ctgatggaaa ctcgcccgat gccgcagtat
    10861 aacgcctttg cgtggttcta tcttggcgcg ttcctggtgg cggtaattgc atggttcttg
    10921 ctgggaaatg cgcagaaaat gccgcaaacc acgttgcaat ggggcattct ggtgtttctt
    10981 ggcgtggtgg cttccgggat tggctacttt atgtggaact acggcgcgac gcaggtggac
    11041 gccggaacgc tgggcattat gaataatatg cacgttccgg cagggctgct ggtaaacctg
    11101 gctatctggc accaacagcc gcactggcca acgtttatta caggcgcgct ggtgatcctg
    11161 gcctcactgt gggtgcatcg taagtgggtc gctccgcgct cttcacaaac ggcagatgat
    11221 cgcaggcgtg attgcgcgct gagcgaataa acgcttccgt aactggctga cgctgctcgc
    11281 catcgcgcac ggcggcgtac agtcggctcc acaagccttc gcccagggtt ttggtcacca
    11341 ccagaccctg gcgctcaaaa ctctctacta cccaatgcgg tagcgcggca atacccatcc
    11401 gcgcggcaac catttgaatc aacaataagg tgttatcgac gcttttcagt gacgggctga
    11461 cgcctgccgg ctgaagaaaa tgccgccaga catccagtcg actacgctgc accggataaa
    11521 ttaatagcgt ctcgctggcg agatcttccg gtgtaatacg cgtttttgcc gccagtggat
    11581 ggtcaggtgc taacaccagg cgtacttcat aatcgaacat cggcgaataa tgcaggccac
    11641 tgcgcggcag gatatcggag gtcatcacca gatccagctc gccctgttgc aacgcgggct
    11701 gcgggtcaaa cgtcacgccc gatttaaaat ccatctctac ctgcggccag ttcttatgga
    11761 aatttcctaa agcgggtgtc agccactgaa tacagctatg gcactcaatg gcaatgcgca
    11821 gacgcgtctg ctgcggttca ttgcaggctt gcagtgcctg gctgatttgc ggcagcacct
    11881 ggtttgccag ttgcaacagg atttctccct gcggtgtaaa gcgtagcggc tggctcttac
    11941 gcacaaatag ccggaagcca aggcgttgtt ccagatcgct aaactggtga gacagggcgg
    12001 attgcgtctg atgcaacgtt gccgcagcgg ctgcgagcga gccgcagttc cgcaacgctt
    12061 gtagcgtttt caggtgtttt acttcgatca tgaaagtcct tcacttcggc atgaataatt
    12121 tgcgcttgag gaatatacag taaccgccaa ttatggatgt gtaaacatct ggacggctaa
    12181 aatcattcgt cttttaaatt tatggtgcgt tggctgcgtt tctccacccc ggtcacttac
    12241 ttcagtaagc tcccggcgat gaataaactt gccgccttcc ctaaattcaa aatccatagg
    12301 atttacatat aattagagga agaaaaatga caatattgaa tcacaccctc ggtttccctc
    12361 gcgttggcct gcgtcgcgag ctgaaaaaag cgcaagaaag ttattgggcg gggaactcca
    12421 cgcgtgaaga actgctgaca gtagggcgtg aactgcgtgc tcgtcactgg gatcaacaaa
    12481 agcaagcggg tatcgacctg ctgccggtgg gcgattttgc ctggtacgat catgtactga
    12541 ccaccagttt gctgctgggt aatgttccgc cacgtcatca gaacaaagac ggttcggtag
    12601 atatcgacac cctgttccgt attggtcgtg gacgtgcgcc gactggcgaa cctgcggcgg
    12661 cagcggaaat gaccaaatgg tttaacacca actatcacta catggtgccg gagttcgtta
    12721 aaggccaaca gttcaaactg acttggacgc agctgctgga ggaagtggac gaggcgctgg
    12781 cgctgagcca caacgttaag cctgtgctgc tggggccggt tacttacctg tggctgggga
    12841 aagtgaaagg tgaacagttt gaccgcctta gcctgctgaa cgacattctg ccggtttatc
    12901 agcaagtgct ggcagaactg gcgaaacgcg gcatcgagtg ggtacagatt gatgaacccg
    12961 cgctggtact ggaactacca caggcgtggc tggacgcata caaacccgct tacgacgcgc
    13021 tccagggaca ggtgaaactg ctgctgacca cctattttga aggcgtaacg ccaaatctcg
    13081 acacgattac tgcgctgcct gttcaggggc tgcatgtgga ccttgtacat ggtaaagata
    13141 acgttgttga actgcacaag cgcctgcctt ctgactggct gctgtctgcg ggtctgatca
    13201 atggtcgtaa cgtctggcgc gccgatctta ctgagaaata tgcgcaaatt aaggacattg
    13261 tcggcaaacg tgatttgtgg gtggcatctt cctgctcgtt gctgcacagc cccatcgacc
    13321 tgagcgtgga aacgcgtctt gatgcagaag tgaaaagctg gtttgccttc gccctacaaa
    13381 aatgccatga actggcactg ctgcgcgatg cgctgaacag tggtgacacg gcagctctgg
    13441 cagagtggag cgccccaatt caggcgcgtc gtcactctac tcgtgtacat aatccggcgg
    13501 tagaaaagcg tctggcggcg atcaccgccc aggacagcca gcgtgcgaat gtctatgaag
    13561 tgcgtgctga agcccagcgt gcgcgtttta aactgccagc gtggccgacc accacgattg
    13621 gttccttccc gcaaaccacg gaaattcgta ccctgcgtct ggatttcaaa aagggcaatc
    13681 tcgacgccaa caactaccgc acgggcattg cggaacatat caagcaggcc attgttgagc
    13741 aggaacgttt gggactggat gtgctggtac atggcgaggc cgagcgtaat gacatggtgg
    13801 aatactttgg cgagcacctc gacggatttg tctttacgca aaacggttgg gtacagagct
    13861 acggttcccg ctgcgtgaag ccaccgattg tcattggtga cattagccgc ccggcaccga
    13921 ttaccgtgga gtgggcgaag tatgcgcaat cgctgaccga caaaccggtg aaagggatgc
    13981 tgacggggcc ggtgaccata ctctgctggt cgttcccgcg tgaagatgtc agccgtgaaa
    14041 ccatcgccaa acagattgcg ctggcgctgc gtgatgaagt ggccgatctg gaagccgctg
    14101 gaattggcat catccagatt gacgaaccgg cgctgcgcga aggtttaccg ctgcgtcgta
    14161 gcgactggga tgcgtatctc cagtggggcg tagaggcctt ccgtatcaac gccgccgtgg
    14221 cgaaagatga cacacaaatc cacactcaca tgtgttattg cgagttcaac gacatcatgg
    14281 attcgattgc ggcgctggac gcagacgtca tcaccatcga aacctcgcgt tccgacatgg
    14341 agttgctgga gtcgtttgaa gagtttgatt atccaaatga aatcggtcct ggcgtctatg
    14401 acattcactc gccaaacgta ccgagcgtgg aatggattga agccttgctg aagaaagcgg
    14461 caaaacgcat tccggcagag cgcctgtggg tcaacccgga ctgtggcctg aaaacgcgcg
    14521 gctggccaga aacccgcgcg gcactggcga acatggtgca ggcggcgcag aatttgcgtc
    14581 gggggtaaat ccaaaccggg tggtaatacc acccggtctt ttctcattac agcgacttct
    14641 tcccaccata ctgcttaaac cattccagca tacgctgcca gccatctttc gcagattcgg
    14701 catgatagct cgggcgataa tcagcgttga atgcatgccc ggcatccggg tacacgataa
    14761 tctctgcttt cgcattagca gcccgcagcg cctggcgcat cgtttcaacg ctctcctgcg
    14821 gaatgctgtt atcctgacca ccatataagc cgagaaccgg cgcgttaaga tcggttgcga
    14881 tatcaacagg ttgtttcggt gaattcagcg acttgtcgcc cgtcagtttg ccgtaccacg
    14941 ccactgcggc ttttagctgt ggattatgcg cggcatacag ccaggtgata cgtccacccc
    15001 agcagaatcc ggtgatcatt aaacgatgaa catcgccgcc gttgcgggaa gcccaactgg
    15061 cgacatgatc gagatcggcc agcacctgcg agtcaggcac ttttgctacc agaccgctaa
    15121 gcaacgtggg gatatcggca aaatcattcg gatcgccttc gcggaagtaa agttcaggtg
    15181 cgatagccag atacccctcc agcgccagac ggcgacaaat gtcgcggata tgttcatgca
    15241 cgccaaaaat ttcctgcact acaatgacca ctggcagtgg gccatcgctt tgctttggtc
    15301 tggcatggta agcaggcatg ttatcccctt gtgaagggat agaggtgaag cccgccacaa
    15361 tcgcgtcgtc cggggtctga acgatggtcg aagcgagagg cgatgcagca ggtgcaaatc
    15421 cagattgttg tgttgttgcc atggtattct ccgtaccctt ataaaaatgt tgcgcaatgt
    15481 taactatagt cagcatgcaa caaatcacat tgcctgaatc ggctcatctt ttatgcagtc
    15541 ctgcagaatg aagagtgatt tatgtgatat gaatcacttt tggtgggtaa atttatgcaa
    15601 cgcatttgcg tcatggtgat gagtatcacg aaaaaatgtt aaacccttcg gtaaagtgtc
    15661 tttttgcttc ttctgactaa accgattcac agaggagttg tatatgtcca agtctgatgt
    15721 ttttcatctc ggcctcacta aaaacgattt acaaggggct acgcttgcca tcgtccctgg
    15781 cgacccggat cgtgtggaaa agatcgccgc gctgatggat aagccggtta agctggcatc
    15841 tcaccgcgaa ttcactacct ggcgtgcaga gctggatggt aaacctgtta tcgtctgctc
    15901 taccggtatc ggcggcccgt ctacctctat tgctgttgaa gagctggcac agctgggcat
    15961 tcgcaccttc ctgcgtatcg gtacaacggg cgctattcag ccgcatatta atgtgggtga
    16021 tgtcctggtt accacggcgt ctgtccgtct ggatggcgcg agcctgcact tcgcaccgct
    16081 ggaattcccg gctgtcgctg atttcgaatg tacgactgcg ctggttgaag ctgcgaaatc
    16141 tattggcgcg acaactcacg ttggcgtgac agtttcttct gataccttct acccaggtca
    16201 ggaacgttac gatacttact ctggtcgcgt agttcgtcac tttaaaggtt ctatggaaga
    16261 gtggcaggcg atgggcgtaa tgaactatga aatggaatct gcaactctgc tgaccatgtg
    16321 tgcaagtcag ggcctgcgtg ccggtatggt agcgggtgtt atcgttaacc gcacccagca
    16381 agagatcccg aatgctgaga cgatgaaaca aaccgaaagc catgcggtga aaatcgtggt
    16441 ggaagcggcg cgtcgtctgc tgtaattctc ttctcctgtc tgaaggccga cgcgttcggc
    16501 cttttgtatt tttgcgtagc gcctcgcagg aaatgccttt ccaactggac gtttgtacag
    16561 cacaattcta ttttgtgcgg gtaagttgtt gcgtcaggag gcgttgtgga tttctcaatc
    16621 atggtttacg cagttattgc gttggtgggt gtggcaattg gctggctgtt tgccagttat
    16681 caacatgcgc agcaaaaagc cgagcaatta gctgaacgtg aagagatggt cgcggagtta
    16741 agcgcggcaa aacaacaaat tacccaaagc gagcactggc gtgcagagtg tgaattgctc
    16801 aataacgaag tgcgcagcct gcaaagtatt aacacctctc tggaggccga tctgcgtgaa
    16861 gtaaccacgc ggatggaagc cgcacagcaa catgctgacg ataaaattcg ccagatgatt
    16921 aacagcgagc agcgcctcag tgagcagttt gaaaacctcg ccaaccgtat ttttgagcac
    16981 agcaatcgcc gggttgatga gcaaaaccgt cagagtctga acagcctgtt gtcgccgcta
    17041 cgtgaacaac tggacggttt ccgccgtcag gttcaggaca gcttcggtaa agaagcacaa
    17101 gaacgccata ccctgaccca cgaaattcgc aatctccagc aactcaacgc gcaaatggcc
    17161 caggaagcga tcaacctgac gcgcgcgctg aaaggcgaca ataaaaccca gggcaactgg
    17221 ggcgaggtag tattgacgcg ggtgctggag gcttccggtc tgcgtgaagg gtatgaatat
    17281 gaaacccagg tcagcatcga aaatgacgcc cgctcgcgga tgcagccgga tgtcatcgtg
    17341 cggctgccgc agggaaaaga tgtggtgatc gacgccaaaa tgacgctggt cgcctatgaa
    17401 cgctatttta acgccgaaga cgactacacc cgcgaaagcg cgctacagga acatatcgcg
    17461 tcggtgcgta accatatccg tttgctggga cgcaaagatt atcaacagct gccggggctg
    17521 cgaactctgg attacgtgct gatgtttatt cccgttgaac ccgctttttt actggcgctt
    17581 gaccgccagc cggagctgat caccgaagcg ttgaaaaaca atatcatgct ggttagcccg
    17641 actacgctgc tggtggcgtt gcgtactatt gccaacctgt ggcgttatga gcatcaaagc
    17701 cgtaacgccc agcaaatcgc cgatcgtgcc agcaagctgt acgacaagat gcgtttgttc
    17761 atcgatgaca tgtccgcgat tggtcaaagt ctcgacaaag cgcaggataa ctaccgccag
    17821 gcaatgaaaa aactctcttc aggtcgcgga aatgtgctgg cgcaggcaga agcgtttcgc
    17881 ggtttaggag tagaaattaa acgcgagatt aatccggatt tggctgaaca ggcggtgagc
    17941 caggatgaag agtatcgact tcggtcggtt ccggagcagc cgaatgatga agcttatcaa
    18001 cgcgatgatg aatataatca gcagtcgcgc tagcccatta ggggtagtta agccgggtag
    18061 aaatctaggg catcgacgcc caatctgtta cacttctgga acaatttttt gatgagcagg
    18121 cattgagatg gtggataagt cacaagaaac gacgcacttt ggttttcaga ccgtcgcgaa
    18181 ggaacaaaaa gcggatatgg tcgcccacgt tttccattcc gtggcatcaa aatacgatgt
    18241 catgaatgat ttgatgtcat ttggtattca tcgtttgtgg aagcgattca cgattgattg
    18301 cagcggcgta cgccgtgggc agaccgtgct ggatctggct ggtggcaccg gcgacctgac
    18361 agcgaaattc tcccgcctgg tcggagaaac tggcaaagtg gtccttgctg atatcaatga
    18421 atccatgctc aaaatgggac gcgagaagct gcgtaatatc ggtgtgattg gcaacgttga
    18481 gtatgttcag gcgaacgctg aggcgctgcc gttcccggat aacacctttg attgcatcac
    18541 catttcgttt ggtctgcgta acgtcaccga caaagataaa gcactgcgtt caatgtatcg
    18601 cgtgctgaaa cccggcggcc gcctgctggt gcttgagttc tcgaagccaa ttatcgagcc
    18661 gctgagcaaa gcctatgatg catactcctt ccatgtgctg ccgcgtattg gctcactggt
    18721 cgcgaacgac gccgacagct accgttatct ggcagaatcc atccgtatgc atcccgatca
    18781 ggataccctg aaagccatga tgcaggatgc cggattcgaa agtgtcgact actacaatct
    18841 gacggcaggg gttgtggcgc tgcatcgtgg ttataagttc tgacaggaga ccggaaatgc
    18901 cttttaaacc tttagtgacg gcaggaattg aaagtctgct caacaccttc ctgtatcgct
    18961 cacccgcgct gaaaacagcc cgctcgcgtc tgctgggtaa agtattgcgc gtggaggtaa
    19021 aaggcttttc gacgtcattg attctggtgt tcagcgaacg ccaggttgat gtactgggcg
    19081 aatgggcagg cgatgctgac tgcaccgtta tcgcctacgc cagtgtgttg ccgaaacttc
    19141 gcgatcgcca gcagcttacc gcactgattc gcagtggtga gctggaagtg cagggcgata
    19201 ttcaggtggt gcaaaacttc gttgcgctgg cagatctggc agagttcgac cctgcggaac
    19261 tgctggcccc ttataccggt gatatcgccg ctgaaggaat cagcaaagcc atgcgcggag
    19321 gcgcaaagtt cctgcatcac ggcattaagc gccagcaacg ttatgtggcg gaagccatta
    19381 ctgaagagtg gcgtatggca cccggtccgc ttgaagtggc ctggtttgcg gaagagacgg
    19441 ctgccgtcga gcgtgctgtt gatgccctga ccaaacggct ggaaaaactg gaggctaaat
    19501 gacgccaggt gaagtacggc gcctatattt catcattcgc acttttttaa gctacggact
    19561 tgatgaactg atccccaaaa tgcgtatcac cctgccgcta cggctatggc gatactcatt
    19621 attctggatg ccaaatcggc ataaagacaa acttttaggt gagcgactac gactggccct
    19681 gcaagaactg gggccggttt ggatcaagtt cgggcaaatg ttatcaaccc gccgcgatct
    19741 ttttccaccg catattgccg atcagctggc gttattgcag gacaaagttg ctccgtttga
    19801 tggcaagctg gcgaagcagc agattgaagc tgcaatgggc ggcttgccgg tagaagcgtg
    19861 gtttgacgat tttgaaatca agccgctggc ttctgcttct atcgcccagg ttcataccgc
    19921 gcgattgaaa tcgaatggta aagaggtggt gattaaagtc atccgcccgg atattttgcc
    19981 ggtcattaaa gcggatctga aacttatcta ccgtctggct cgctgggtgc cgcgtttgct
    20041 gccggatggt cgccgtctgc gcccaaccga agtggtgcgc gagtacgaaa agaccttgat
    20101 tgatgaactg aatttgctgc gggaatctgc caacgccatt cagcttcggc gcaattttga
    20161 agacagcccg atgctctaca tcccggaagt ttaccctgac tattgtagtg aagggatgat
    20221 ggtgatggag cgcatttacg gcattccggt gtctgatgtt gcggcgctgg agaaaaacgg
    20281 cactaacatg aaattgctgg cggaacgcgg cgtgcaggtg ttcttcactc aggtctttcg
    20341 cgacagcttt ttccatgccg atatgcaccc tggcaacatc ttcgtaagct atgaacaccc
    20401 ggaaaacccg aaatatatcg gcattgattg cgggattgtt ggctcgctaa acaaagaaga
    20461 taaacgctat ctggcagaaa actttatcgc cttctttaat cgcgactatc gcaaagtggc
    20521 agagctacac gtcgattctg gctgggtgcc accagatacc aacgttgaag agttcgaatt
    20581 tgccattcgt acggtctgtg aacctatctt tgagaaaccg ctggccgaaa tttcgtttgg
    20641 acatgtactg ttaaatctgt ttaatacggc gcgtcgcttc aatatggaag tgcagccgca
    20701 actggtgtta ctccagaaaa ccctgctcta tgtcgaaggg gtaggacgcc agctttatcc
    20761 gcagctcgat ttatggaaaa cagcgaagcc tttcctggag tcgtggatta aagatcaggt
    20821 cggtattcct gccctggtga gagcatttaa agaaaaagcg ccgttctggg tcgaaaaaat
    20881 gccagaactg cctgaactgg tttacgacag tttgcgccag ggcaagtatt tacagcacag
    20941 tgttgataag attgcccgcg agcttcagtc aaatcatgta cgtcagggac aatcgcgtta
    21001 ttttctcgga attggcgcta cgttagtatt aagtggcaca ttcttgttgg tcagccgacc
    21061 tgagtggggg ctgatgcccg gctggttaat ggcaggtggt ctgatcgcct ggtttgtcgg
    21121 ttggcgcaaa acacgctgat tttttcatcg ctcaaggcgg gccgtgtaac gtataatgcg
    21181 gctttgttta atcatcatct accacagagg aacatgtatg ggtggtatca gtatttggca
    21241 gttattgatt attgccgtca tcgttgtact gctttttggc accaaaaagc tcggctccat
    21301 cggttccgat cttggtgcgt cgatcaaagg ctttaaaaaa gcaatgagcg atgatgaacc
    21361 aaagcaggat aaaaccagtc aggatgctga ttttactgcg aaaactatcg ccgataagca
    21421 ggcggatacg aatcaggaac aggctaaaac agaagacgcg aagcgccacg ataaagagca
    21481 ggtgtaatcc gtgtttgata tcggttttag cgaactgcta ttggtgttca tcatcggcct
    21541 cgtcgttctg gggccgcaac gactgcctgt ggcggtaaaa acggtagcgg gctggattcg
    21601 cgcgttgcgt tcactggcga caacggtgca gaacgaactg acccaggagt taaaactcca
    21661 ggagtttcag gacagtctga aaaaggttga aaaggcgagc ctcactaacc tgacgcccga
    21721 actgaaagcg tcgatggatg aactacgcca ggccgcggag tcgatgaagc gttcctacgt
    21781 tgcaaacgat cctgaaaagg cgagcgatga agcgcacacc atccataacc cggtggtgaa
    21841 agataatgaa gctgcgcatg agggcgtaac gcctgccgct gcacaaacgc aggccagttc
    21901 gccggaacag aagccagaaa ccacgccaga gccggtggta aaacctgctg cggacgctga
    21961 accgaaaacc gctgcacctt ccccttcgtc gagtgataaa ccgtaaacat gtctgtagaa
    22021 gatactcaac cgcttatcac gcatctgatt gagctgcgta agcgtctgct gaactgcatt
    22081 atcgcggtga tcgtgatatt cctgtgtctg gtctatttcg ccaatgacat ctatcacttg
    22141 gtatccgcgc cattgatcaa gcagttgccg caaggttcaa cgatgatcgc caccgacgtg
    22201 gcctcgccgt tctttacgcc gatcaagctg acctttatgg tgtcgctgat tctgtcagcg
    22261 ccggtgattc tctatcaggt gtgggcattt atcgccccag cgctgtataa gcatgaacgt
    22321 cgcctggtgg tgccgctgct ggtttccagc tctctgctgt tttatatcgg catggcattc
    22381 gcctactttg tggtctttcc gctggcattt ggcttccttg ccaataccgc gccggaaggg
    22441 gtgcaggtat ccaccgacat cgccagctat ttaagcttcg ttatggcgct gtttatggcg
    22501 tttggtgtct cctttgaagt gccggtggca attgtgctgc tgtgctggat ggggattacc
    22561 tcgtcagaag acttacgcaa aaaacgcccg tatgtgctgg ttggtgcatt cgttgtcggg
    22621 atgttgctga cgccgccgga tgtcttctcg caaacgctat tggcgatccc gatgtactgt
    22681 ctgtttgaaa tcggtgtctt cttctcacgc ttttacgttg gtaaagggcg aaatcgggaa
    22741 gaggaaaacg acgctgaagc agaaagcgaa aaaactgaag aataaattca accgcccgtc
    22801 agggcggttg tcatatggag tacaggatgt ttgatatcgg cgttaatttg accagttcgc
    22861 aatttgcgaa agaccgtgat gatgttgtag cgcgcgcttt tgacgcggga gttaatgggc
    22921 tactcatcac cggcactaac ctgcgtgaaa gccagcaggc gcaaaagctg gcgcgtcagt
    22981 attcgtcctg ttggtcaacg gcgggcgtac atcctcacga cagcagccag tggcaagctg
    23041 cgactgaaga agcgattatt gagctggccg cgcagccaga agtggtggcg attggtgaat
    23101 gtggtctcga ctttaaccgt aacttttcga cgccggaaga gcaggaacgc gcttttgttg
    23161 ctcagctacg cattgccgca gaattaaaca tgccggtatt tatgcactgt cgcgatgccc
    23221 acgagcggtt tatgacattg ctggagccgt ggctggataa actgcctggt gcggttcttc
    23281 attgctttac cggcacacgc gaagagatgc aggcgtgcgt ggcgcgtgga atttatatcg
    23341 gcattaccgg ttgggtttgc gatgaacgac gcgggctgga gctgcgggaa ttgttgccgt
    23401 tgattccggc ggagaaattg ctgatcgaaa cagatgcgcc gtatctgctc ccgcgcgatc
    23461 tcacgccaaa gccatcatcc cggcgcaacg agccagccca tctgccccat attttgcaac
    23521 gtattgcgca ctggcgtgga gaagatgccg catggctggc tgccaccacg gatgccaatg
    23581 tcaaaacact gtttgggatt gcgttttaga gtttgcggaa ctcggtgttc ttcacactgt
    23641 gcttaatctc tttattaata agattaagca atagcatgga gcgagcctca ccatcgggtt
    23701 cggtgaaaat ggcctgaaag ccttcgaacg cgccttcggt aataatcacc ttatcacccg
    23761 gataaggggg ggccggatcg acaatgtctt tcggtttata taccgatagc tgatgaatta
    23821 ccgccgatgg gactatcgct ggcgacgcgc caaagcgcac aaagtggctg acgccacgcg
    23881 tagcgctgat ggtcgtggta tgaatcactt ctggatcaaa ttccacaaac aggtagttag
    23941 ggaataacgg ttcactgact gcggtacgtt taccccgtac gattttttcc agggtgatca
    24001 tcggagcgag gcaattaacc gcctgtctct caaggtgttc ctgggcacgt tgaagttgcc
    24061 cgcgcttgca gtacagtaaa taccaggatt gcataatgac tcttatccgt ttaatcgggg
    24121 cgcaaggata gcaaaagctt tacgctaagt taattatatt ccccggtttg cgttataccg
    24181 tcagagttca cgctaattta acaaatttac agcattgcaa agatgaacgc cgtataatgg
    24241 gcgcagatta agaggctaca atggacgcca tgaaatataa cgatttacgc gacttcctga
    24301 cgctgcttga acagcagggt gagctaaaac gtatcacgct cccggtggat ccgcatctgg
    24361 aaatcactga aattgctgac cgcactctgc gtgctggtgg gcctgcgctg ttgttcgaaa
    24421 accctaaagg ctactcaatg ccggtgctgt gcaacctgtt cggtacgcca aagcgcgtgg
    24481 cgatgggcat ggggcaggaa gatgtttcgg cgctgcgtga agttggtaaa ttattggcgt
    24541 ttctgaaaga gccggagccg ccaaaaggtt tccgcgacct gtttgataaa ctgccgcagt
    24601 ttaagcaggt gttaaacatg ccgacaaagc gactgcgtgg tgcgccctgc caacaaaaaa
    24661 tcgtctctgg cgatgacgtc gatctcaatc gcattcccat tatgacctgc tggccggaag
    24721 atgccgcgcc gctgattacc tgggggctga ccgtaacgcg cggcccgcat aaagagcggc
    24781 agaatctggg catttatcgc cagcagctga ttggtaaaaa caaactgatt atgcgctggc
    24841 tgtcgcatcg cggcggcgcg ctggattatc aggagtggtg tgcggcgcat ccgggcgaac
    24901 gtttcccggt ttctgtggcg ctgggtgccg atcctgccac gattctcggt gcagtcaccc
    24961 ccgttccgga tacgctttca gagtatgcgt ttgccggatt gctacgcggc accaaaaccg
    25021 aagtagtaaa gtgtatttcc aatgacctcg aagtgccagc cagtgccgaa atcgtcctgg
    25081 aagggtatat cgatcctggt gagatggcgc cggaagggcc gtatggtgac cacacaggtt
    25141 actataatga agtcgataat ttcccggtgt ttaccgtgac gcatattacc cagcgtgaag
    25201 atgcgattta ccattccacc tataccgggc gtccgccaga tgagcccgcg gtactgggcg
    25261 tggcgttgaa cgaagtgttt gtaccgattc tgcaaaaaca gttcccggaa attgtcgatt
    25321 tttacctgcc gccggaaggc tgttcttatc gtctggcggt agtgacgatc aaaaaacagt
    25381 acgccggaca cgcgaagcgc gtcatgatgg gcgtctggtc gttcttacgc cagtttatgt
    25441 acactaaatt tgtgatcgtt tgcgatgatg acgtcaacgc acgcgactgg aacgatgtga
    25501 tttgggcgat taccacccgt atggacccgg cgcgggatac tgttctggta gaaaatacgc
    25561 ctattgatta tctggatttt gcctcgcctg tctctgggct gggttcaaaa atggggctgg
    25621 atgccacgaa taaatggccg ggggaaaccc agcgtgaatg gggacgtccc atcaaaaaag
    25681 atccagatgt tgtcgcgcat attgacgcca tctgggatga actggctatt tttaacaacg
    25741 gtaaaagcgc ctgatgcgcg tttgttttgc cctatttatc gatccgacag agaaagcgca
    25801 tgacaacctt aagctgtaaa gtgacctcgg tagcagctat cacggatacc gtatatcgtg
    25861 tccgcatcgt gccagacgcg gccttttctt ttcgtgctgg tcagtatttg atggtagtga
    25921 tggatgagcg cgacaaacgt ccgttctcaa tggcctcgac gccggatgaa aaagggttta
    25981 tcgagctgca tattggcgct tctgaaatca acctttacgc gaaagcagtc atggaccgca
    26041 tcctcaaaga tcatcaaatc gtggtcgacc ttccccacgg agaagcgtgg ctgcgcgatg
    26101 atgaagagcg tccgatgatt ttgattgcgg gcggcaccgg gttctcttat gcccgctcga
    26161 ttttgctgac agcgttggcg cgtaacccaa accgtgatat caccatttac tggggcgggc
    26221 gtgaagagca gcatctgtat gatctctgcg agcttgaggc gctttcgtta aagcaccttg
    26281 gtctgcaagt ggtgccggtg gttgaacaac cggaagcagg ctggcgtggg cgtactggta
    26341 ccgtgttaac ggcggtattg caggatcatg gtacgctggc agagcatgat atctatattg
    26401 ccggacgttt tgagatggcg aaaattgccc gtgacctgtt ttgcagtgag cgtaatgcgc
    26461 gggaagatcg cctgtttggc gatgcgtttg catttatctg agatataaaa aaacccgccc
    26521 ctgacaggcg ggaagaacgg caactaaaac tttattcagt ggcatttaga tctatgtcgc
    26581 atccggcaat tggtgcacga tgcctgatgc gacgctggcg cgtcttatca ggcctacatt
    26641 ggtgccggat cggtagaccg gataaggcgt tcacgccgca tccggcaagt ggttaaaccc
    26701 gctcaaacac cgtcgcaata ccctgaccca gaccgataca catcgtcgcc agaccaaact
    26761 gaacgtcttt gcgttccatc agattcagca gcgtggtgct gatacgcgca ccggaacaac
    26821 ccagcggatg acccagcgcg atcgcaccac cattgaggtt gatcttctcg tcaatctgct
    26881 ccattagccc gagatcttta atgcacggca ggatctgcgc ggcaaaggct tcgttcatct
    26941 caaacacgcc gatatcgctg gcagaaagcc ccgctttttt cagcgccagc ttcgaggccg
    27001 gaaccgggcc gtaacccata atcgatgggt cacaaccaac gaccgccatc gaacgcacac
    27061 gagcgcgcgg cttaagacct aattcatggg cgcggctttc actcatcacc agcatggcag
    27121 ctgcgccatc ggaaagtgca gaagatgtgc ccgccgttac cgtaccgttt actggatcaa
    27181 acgccggacg cagcgtggcg agggcttcca cggtggtttc cgggcgaatc acttcgtcgt
    27241 aattaaactg cttcaggacg ccgtcggcat cgtgaccacc agttgggatg atttcatttt
    27301 taaatgcggc cgactgcgtg gcggcccagg cgcgggcgtg tgaccgcgcg gcaaaggcat
    27361 cctgcatttc acggctgata ccgtgcatac gcgccagcat ttctgccgtt aagcccatca
    27421 tgcccgccgc tttggcgaca ttgcggctca ggccggggtg aaaatcgacg ccgtgactca
    27481 tcggcacatg gcctatatgc tccacgccgc caaccagaca tgcctgcgta tcgccagtca
    27541 tgatcattcg tgctgcgtca tgcagtgcct gcatggatga accacacaag cgattgacgg
    27601 taaccgccgg gacagagtgc ggcacttctg ccagcagcgc cgcgttacgg gcgatgttaa
    27661 aaccctgctc cagcgtctgt tgcacacaac cccagtaaat atcgtcgagg gccgccgctt
    27721 ccagcgccgg gttacgcgcc agcaggctac gcattaaatg agcggagaga tcttccgcac
    27781 gcacgttacg aaaagcaccg cccttcgaac ggcccatcgg ggtgcgaatt gcatcgacaa
    27841 tgacaacctg ttccattgtg actccttaag ccgttttcag gtcgccaacc ggacgggctg
    27901 gctcaaccgg aggatagtac ggttcgttat gacgcgcttt attacgcaga ccttccggca
    27961 cttcatacag cgggccgagg tgctgatatt gctgtgccat atcgaggtat tttgcgctac
    28021 cgagggtgtc cagccagcgg aacgcgccgc cgtggaacgg agggaagccc aggccgtaga
    28081 ccagcgccat atccgcttcc gccggagtgg cgataatgcc ctcttccaga cagcgcacca
    28141 cttcgttgac catcgggatc atcatgcggg cgataatctc ttcttcgctg aaatcgcgct
    28201 tcggttggct cacttctgcc agcaggtctt caacggcggc gtcttcttct ttcttcggct
    28261 tacctttgct gtcttcttta taacgccaga aaccgaggcc gttcttctga ccaaagcggt
    28321 tggcatcaaa cagcgcgtcg atggcatcgc ggtaatcttt ctgcatccgc tgcgggaagc
    28381 ctgctgccat gacagcctga gcgtgatgcg cggtatcaat gcccacaacg tccagcagat
    28441 atgccgggcc catcggccag ccaaactgtt tttccatcac tttgtcgatc ttgcggaaat
    28501 ccgcgccgtc gcgcagcagc tggctgaaac cggcgaaata cgggaacagc acgcggttaa
    28561 caaagaagcc ggggcagtcg ttaaccacaa tcggcgtctt gcccatcttg ctcgcccagg
    28621 cgacaacttt cgcgatggtt tcgtcggagc ttttctcgcc gcgaataatt tctaccaacg
    28681 gcattcggtg gaccgggtta aagaagtgca tcccgcagaa gttttccggg cgttccagcg
    28741 cgttggccag ttcgctgata ggaatggttg aagtgttaga cgccagcacg gtatcctggc
    28801 gtactttttg ttcggtttct gccagtacgg cttttttcac tttcgggttt tcaacaaccg
    28861 cttctaccac aatatccacg cggtcaaatc cggcgtagtc gagcgttggg tggatcgtgg
    28921 agatcacgcc agccagtttc agaccatcga ttttgccgcg ctcaagctgc ttgttcagca
    28981 gtttcgcggc ttcggtcatg ccgagggtta acgacttgtc gttgatatct ttcatgacaa
    29041 ccggcacgcc tttccacgcg gactggtaag cgataccgcc acccatgatg cctgcgccca
    29101 gcacagcagc ctgtttcggg gtttcaacgt ctttggtgag tttcttcgct ttgcctttta
    29161 catattgatc gttaaggaaa atgccgacca gtgcgcgagc ttcgttggta tgcgccagcg
    29221 ggacaaaact tttgttttcc agatttaagg cttcttcacg accaaaacgg gccgcagctt
    29281 caatggtttt tactgcggtg atgggggccg gataatgttt ccccgccgtt tgcgcgacca
    29341 ttcctttagc gatggtgaag ctcatggtgg cttcaatctt gctcagtttt agcggttcct
    29401 gcttcggctg acgtttcgct ttccagtcga ggtcgccgtt aatggcctgg cgtaaaatcg
    29461 ccatcgcccc ttcaatcagt ttttctgctt tgactacgcc atccaccaga ccgattttca
    29521 gcgcctgatc cgcgccgaca tctttaccgg cggcaatgat ttccagtgca ctgtcagcgc
    29581 ccagcatacg tggcatacgt acagaaccgc caaagcccgg catgatgccc agtttggttt
    29641 ccggcagacc gatgcgcaga tccggcgtcg ccagacgata atcggtcgcc agcacgcatt
    29701 cgcagccacc acccagcgca tagccgttga cggcagcaat ggtcggcacc ggcagatctt
    29761 ccaggcgatt aaacacgcta ttggcaaagt gcagccactg acttaactgt tcttcaggaa
    29821 cgaggaacag ggacaaaaat tcggtgatat cagcaccgac gataaaggct gctttgttcg
    29881 aacgcagcag cagccctttt agatctgatt gctgttccag cacgccgatg gcctcgccga
    29941 ggctggcgac ggtcgcagtg tcgagtttat taactgaacc tggggcatca aataccagtt
    30001 cggcaatgcc atcttccagc cagtcaaggt acagggtgtc gcctttgtaa agcatgtcag
    30061 tctcctgaat ccgcaaggta atctggtcgt accagatgca tcggagtgtg tgttttgtgt
    30121 taaaaatatg caaataaaag attaaagaaa tgccgatctg atcacgctcg gcagaaatca
    30181 cgctctggat gaacgatgtg ctaagatgcg gagacttaag gtcaaaaaaa cagaagggta
    30241 aaaaatggaa tcactggcct cgctctataa aaatcatata gctaccttac aagaacggac
    30301 tcgcgatgcg ctggcgcgct tcaagctgga tgcattactt attcactccg gcgagctgtt
    30361 caacgttttt ctcgacgatc atccctatcc gtttaaagtg aacccgcaat tcaaagcgtg
    30421 ggtgccggta actcaggtgc caaactgctg gttgctggtg gatggcgtga acaagccgaa
    30481 actgtggttc tatctgccgg ttgattactg gcacaacgtc gaaccgctgc cgacctcctt
    30541 ctggactgaa gatgtggaag tgatcgcgct gccgaaagcc gatggcattg gtagcctgct
    30601 gcctgctgcg cgcggcaata tcggttatat cggtccggtg ccggaacgtg cgctgcaact
    30661 gggtattgag gccagcaaca tcaacccgaa aggggtgatc gactacctgc attattaccg
    30721 ctccttcaaa accgagtacg aactggcctg tatgcgtgaa gcgcagaaaa tggcggtcaa
    30781 tggtcatcgt gcggcagaag aagcgttccg ttctggcatg agcgagttcg atatcaatat
    30841 tgcctatctg accgcgaccg gtcatcgtga taccgacgta ccttacagca acattgtggc
    30901 gcttaacgaa cacgctgcgg tgttgcatta caccaaactg gaccaccagg caccggaaga
    30961 gatgcgcagc ttcctgctgg atgccggggc cgaatataac ggctatgcgg ctgacctgac
    31021 ccgtacctgg tcggcaaaaa gcgacaacga ctacgcacag ctggtgaaag acgtaaatga
    31081 tgaacaactg gcgctgatcg ccaccatgaa agcaggcgtc agctatgtgg attaccacat
    31141 ccagttccat cagcgcatcg ccaaactgct gcgtaaacat caaatcatca ccgatatgag
    31201 tgaagaagcg atggtcgaaa acgatctcac cggaccgttt atgccgcacg gtattggcca
    31261 tccgctgggc ctgcaggtgc atgacgtcgc cggttttatg caggatgata gcggtacaca
    31321 cctcgcggca ccggcaaaat atccgtacct gcgctacacc cgtattctcc agccgggcat
    31381 ggtgttaacc atcgaaccgg gtatctactt cattgaatcg cttctggcac cgtggcgtga
    31441 agggcagttc agcaagcact tcaactggca gaaaattgaa gcactgaaac cgttcggcgg
    31501 cattcgtatc gaagacaacg tggtgatcca cgaaaacaac gtggaaaaca tgacccggga
    31561 tctgaaactg gcgtgatgga aagctggtta attcctgcgg caccggtcac ggtcgttgaa
    31621 gagatcaaaa agagccgctt cataacgatg ttggcgcata ccgatggcgt tgaggcggcg
    31681 aaagcgtttg ttgaatcggt gcgggcagaa caccccgatg cccgccacca ttgcgtggcg
    31741 tgggtggcgg gtgcgccgga tgattctcaa cagctgggtt tctctgacga cggggagccg
    31801 gcgggaacgg caggtaaacc gatgctcgcc cagctaatgg gcagcggcgt cggggaaatt
    31861 accgctgtgg tagtgcgcta ctacggcggc atattgcttg gcaccggtgg gttagtgaaa
    31921 gcgtatggcg gcggcgtgaa tcaggcgctg cgccagctaa cgacccaacg caagacgcca
    31981 ttaaccgaat atactttgca atgtgaatat agtcagttaa ccggcattga agcgttgctg
    32041 gggcagtgtg acggcaaaat tatcaacagt gattatcagg cattcgttct gctgcgggtg
    32101 gcgcttccgg cggcgaaagt ggctgaattt tccgcaaagc tggcggattt tagccgtggt
    32161 tcattgcaat tgttagcgat tgaagaataa tccccacttc gttttgcaga actaaggaag
    32221 cggcagagat gcattttcgc gccattaccc gaatcgttgg actactggtc atcttatttt
    32281 cagggaccat gattatccct gggctggtag cactcatcta ccgggatgga gcgggccgcg
    32341 cttttaccca gacctttttt gtcgccctcg ccattggctc tatgctgtgg tggccgaacc
    32401 gcaaagagaa gggcgaactg aaatcccgtg aggggtttct gatagtggtg ctgttttgga
    32461 ccgtgctggg tagtgtcggt gcgctccctt ttatcttctc ggaaagcccg aacctcacga
    32521 ttaccgatgc gttttttgaa tctttctctg gcctgaccac caccggggcc actacgctgg
    32581 tggggctgga ttcgctccct catgccatcc tcttttatcg ccagatgctg caatggtttg
    32641 gcgggatggg gatcatcgtg ttagcggttg cgatactgcc tatcctcggc gtgggtggga
    32701 tgcagctcta tcgcgcagaa atgcccggcc cgctgaaaga taacaaaatg cgcccgcgaa
    32761 ttgcggaaac ggcgaaaact ctgtggttga tttatgtctt gctgaccgtc gcctgtgcgc
    32821 tggcgttgtg gtttgccgga atggatgcct ttgatgccat cggccatagc tttgcgacta
    32881 tcgctattgg cggcttctcg acacatgatg ccagtatcgg ttatttcgac agtccgacta
    32941 ttaacactat cattgctatc ttcctgctga tctccggctg taactacggt ctgcactttt
    33001 cactgttaag tgggcgtagt ctgaaggttt attggcgcga tccggaattt cgcatgttta
    33061 tcggcgtaca gtttacgctg gtggttattt gtacactcgt actgtggttt cataatgcct
    33121 acagttcggc gctgatgaca attaaccagg cgtttttcca ggtggtatcg atggcgacaa
    33181 ccgccgggtt tacgactgac agcattgccc gctggccgct ctttttgccg gtactgcttt
    33241 tatgttcagc ttttatcggc ggttgcgccg ggtcaacggg cggtggcctg aaagtgatcc
    33301 gcatcctgct gctgtttaag caggggaacc gtgagctgaa acgactggtg catccgaacg
    33361 ccgtctatag cattaagctg gggaatcgcg cactgccgga acgtatcctc gaagccgttt
    33421 ggggattttt ctccgcctat gcattggtgt ttattgtcag tatgctggcg attatcgcca
    33481 cgggcgtgga tgatttttct gcctttgcct cagttgttgc gacattgaat aacctggggc
    33541 cagggcttgg cgtggttgct gataacttta ccagtatgaa cccggtggct aaatggatcc
    33601 tgattgccaa catgctgttt ggtcgtctcg aggtctttac attactggtg ctctttaccc
    33661 cgactttctg gcgtgaatga tggagtaata cgtgaaaaca ttaattcttt tctcaacaag
    33721 ggacggacaa acgcgcgaga ttgcctccta cctggcttcg gaactgaaag aactggggat
    33781 ccagacggat gtcgccaatg tgcaccgcat tgaagaacca caatgggaaa actatgaccg
    33841 tgtggtcatt ggtgcttcta ttcgctatgg tcactaccac tctgcgttcc aggaatttgt
    33901 caaaaaacat gcgacgcgac tgaattcgat gccgagcgcc ttttattccg taaacctggt
    33961 ggcgcgcaaa ccggagaagc gtactccaca gaccaacagc tacgcgcgga agtttctgat
    34021 gaactcgcaa tggcgtcccg atcgctgcgc ggtcattgcc ggggcgctgc gttacccacg
    34081 ttatcgctgg tacgaccgtt ttatgctcaa gctgattatg aagatgtcag gcggtgaaac
    34141 ggatacgcgc aaagaagttg tctataccga ttgggagcag gtggcgaatt tcgcccgaga
    34201 aatcgcccat ttaaccgaca aaccgacgct gaaataagca taaagaataa aaaatgcgcg
    34261 gtcagaaaat tattttaaat ttcctcttgt caggccggaa taactcccta taatgcgcca
    34321 ccactgacac ggaacaacgg caaacacgca gccgggtcag cggggttctc ctgagaattc
    34381 cgacagagaa agcaaaaata aatgcttgac tttgtagcgg gaaagtgtat tatgaacacc
    34441 ccgcgc
//
LOCUS       DAJTSA010000036        33153 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000036 DAJTSA010000000
VERSION     DAJTSA010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.036"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(2..94)
                     /locus_tag="OO369_001863"
     tRNA            complement(2..94)
                     /locus_tag="OO369_001863"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO369_001864"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO369_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR5982162.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO369_001865"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO369_001865"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR5982163.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO369_001866"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO369_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR5982164.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO369_001867"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO369_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR5982165.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO369_001868"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO369_001868"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR5982166.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO369_001869"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO369_001869"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR5982167.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="OO369_001870"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="OO369_001870"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="OO369_001871"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="OO369_001871"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR5982168.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="OO369_001872"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="OO369_001872"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR5982169.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="OO369_001873"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="OO369_001873"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR5982170.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="OO369_001874"
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="OO369_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR5982171.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="OO369_001875"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="OO369_001875"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR5982172.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="OO369_001876"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="OO369_001876"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR5982173.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO369_001877"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO369_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR5982174.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="OO369_001878"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="OO369_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR5982175.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="OO369_001879"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="OO369_001879"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR5982176.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO369_001880"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO369_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR5982177.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO369_001881"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO369_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR5982178.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO369_001882"
     CDS             complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO369_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR5982179.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20900..21019)
                     /locus_tag="OO369_001883"
     CDS             complement(20900..21019)
                     /locus_tag="OO369_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HCR5982180.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO369_001884"
     CDS             complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO369_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR5982181.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO369_001885"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO369_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR5982182.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..25505)
                     /gene="hycC"
                     /locus_tag="OO369_001886"
     CDS             complement(23682..25505)
                     /gene="hycC"
                     /locus_tag="OO369_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005092744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR5982183.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALRRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGIILLGLGLGLGLGLGLGLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSLF
                     KSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWV
                     IYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENA
                     TCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIA
                     CPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLR
                     KWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(25542..26153)
                     /gene="hycB"
                     /locus_tag="OO369_001887"
     CDS             complement(25542..26153)
                     /gene="hycB"
                     /locus_tag="OO369_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR5982184.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(26278..26739)
                     /gene="hycA"
                     /locus_tag="OO369_001888"
     CDS             complement(26278..26739)
                     /gene="hycA"
                     /locus_tag="OO369_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR5982185.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            26951..27301
                     /gene="hypA"
                     /locus_tag="OO369_001889"
     CDS             26951..27301
                     /gene="hypA"
                     /locus_tag="OO369_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5982186.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            27305..28177
                     /gene="hypB"
                     /locus_tag="OO369_001890"
     CDS             27305..28177
                     /gene="hypB"
                     /locus_tag="OO369_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR5982187.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            28168..28440
                     /gene="hypC"
                     /locus_tag="OO369_001891"
     CDS             28168..28440
                     /gene="hypC"
                     /locus_tag="OO369_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR5982188.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            28440..29561
                     /gene="hypD"
                     /locus_tag="OO369_001892"
     CDS             28440..29561
                     /gene="hypD"
                     /locus_tag="OO369_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR5982189.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            29558..30568
                     /gene="hypE"
                     /locus_tag="OO369_001893"
     CDS             29558..30568
                     /gene="hypE"
                     /locus_tag="OO369_001893"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR5982190.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            30642..32720
                     /gene="flhA"
                     /locus_tag="OO369_001894"
     CDS             30642..32720
                     /gene="flhA"
                     /locus_tag="OO369_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR5982191.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(32757..33110)
                     /locus_tag="OO369_001895"
     CDS             complement(32757..33110)
                     /locus_tag="OO369_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR5982192.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
BASE COUNT         7811 a         9245 c         8627 g         7470 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggccggcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctggcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgcctg gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtgga ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagattgttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcagcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgggcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactag catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat accgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacactcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 aggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctaccggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    23881 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    23941 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    24001 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    24061 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    24121 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    24181 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    24241 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    24301 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    24361 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    24421 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    24481 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    24541 accaggccaa gagcaatcag cgccggttgt tcgagcgcga tacccgttac gccagcgccc
    24601 agccccagcc ccagccccag ccccagcccc agccccagcc ccagccccag caggataatg
    24661 ccgatatttt ccagggtgtg gtaagccagc aggcgctgga tgttgtgttc catcaacgca
    24721 tacagaccac cgacgaacgc ggtgatcatg ccgagcacca gcagcgcgat cccccaccac
    24781 agcggtgcat taccgcccag cagtgacagg gttaacatgc ccagcaggcc aattttcatg
    24841 actaccgtag aaaacaacgc ggcagctggc gcagaggcgt tagcatgtgc ctgcggcacc
    24901 cagccgtgca gcggaataat cccagccagc aggccaaagc caatcacacc gagcagccag
    24961 atatcggaac cgagcggcag ctgttgcata cgcatatcca gcaggcgcag atccagcgtg
    25021 ccataacgct gccacagcag ccagcaagca atcaccagca gcagagtgcc aagacgcctc
    25081 agcgcaaacc acagtttgcc ctctttgctg ttgctggtga ggaacaccgc acacaaggcc
    25141 atgacttcgg ccattaccac gaacatgccg aggttgctgg cgatgacggc gcagacggcg
    25201 gcagccatca acatattgat ctgcaagccg ttgcacttca cctgcgcgtg gcgatgccag
    25261 tcaatgttat agaggctgac aaacagaccg caaaggccga gggtaatcag ccaaatcgcg
    25321 ttaagcgggg agatttgcac atcgtagctt accagcgaca gcgcaccgct cacgccaacc
    25381 gcgccagtca gtacagtgaa gcccgcggct gccgtataca gactaccaac cgcgccgcca
    25441 attccggcta tccagccact tagcgctttt tgaaaagaga aatgccagaa cagcggcggc
    25501 gacaaaccat gccacgccgc tattgatcag ggaaattgcg ctcatttagc ctctccactt
    25561 tgagcctgct gaaacaaggt gagatcgcca aagtccgtgt taaaggtcag ctcacgctta
    25621 cgtttgctga cgcgggcgat atcggtgtta tccaccagat gcagggcttt agtcgggcac
    25681 atccgcacac aggccggacc ttgttcatca aagctacaaa ggtcacattt gacggcgatc
    25741 gcgcgaatac ctggcaccca gtcaagcaat gtgctgacac gcgccggagc aggcggtgcc
    25801 ggtggcgctt tcggggtatt ggcgtttgcc ggaatatcca gcggacggct gccggaaaat
    25861 tcaattgcgc caaacgggca ggcgatgccg cacagtttgc agcttacgca cagactttca
    25921 ttcaactgca cggccccatc aacgcgggtg atggcgttaa ccgggcagac caccgcgcag
    25981 ggtgcatctt cacagtggtg acagagctgc ggcgcagatt ctttttcatt gagcatcact
    26041 ctcaggcgcg gcattgattg caggccgtgc tggcgatgcg tctctgaaca ggcggcctca
    26101 caggtgtggc agccgataca gagcgtggag tcggcaatta caaaacgatt caccaggcat
    26161 tcctcaggtg attgtcattt ttgacgaaaa catgccgatg aaatgtcatt ttcgacactc
    26221 atcgacacgc ccatccccaa acaggcgtaa cgcctgcaaa acgggcaaag cctcagctca
    26281 tgctgccggg ctttgtccct ttaccagttg gcttaaattc accggcacat tgttttcaat
    26341 ggcggtgtat aagctgtcgt aaacgccagc gattttttcg agatagtgtt ccagaacctg
    26401 ttcgcgatcg cggatgttga ccttgccatc aatcatgccg tcaatgctca gttgcgcctg
    26461 cgcaatccgc tgtttgtctt cgccatcttt tgtttcgacg taatactcga tggtgtggct
    26521 gttaaagcca tccgccaggg tgacgtaaat acgaaaatgt tcaaaaagga cgaaacagag
    26581 ttctttgtcc ggcgcgcagc tcatggcgtg atgcaggcgc gctttcgata acgtgatccc
    26641 ctgaaccagc gaattgcagt agatgtgcca ctggtcctgt aggcgacgat gccgctgtgc
    26701 gatgtaatcg gctttctcgc ttatttccca aatagtcatt gtcaggttac tcgtttaaca
    26761 gagatgccag ctttaagcat tttttgtgcc aacttttaat ttattgttat taaagagatt
    26821 tttaagctaa agatgaattt cgtcgccgtg tcgacgtgtc atttcgacat catcgacatt
    26881 attcaccgca gggataatca acactggcgc aattattgct tgtagctggc aatagttaat
    26941 gggaggcgat atgcacgaaa taaccctctg ccaacgggca ctggaattga tcgaacagca
    27001 ggccgcaaaa cacggcgcaa aacgcgtaac tggggtctgg ctcaaaattg gcgcattttc
    27061 ttgtgtcgaa accagctctc ttgccttttg ttttgatctg gtttgccgcg gcagcgtggc
    27121 ggaaggttgt aaactgcacc tcgaagaaca agaggccgaa tgctggtgtg aaacatgcca
    27181 acagtatgtg acgctactga cccagcgcgt ccgccgctgt ccacagtgtc atggtgacat
    27241 gctacagatt gtggcagacg acggtttaca gattcggcgg atagaaatag accaggagtg
    27301 agcgatgtgt acaacatgcg gttgcggtga aggcaacctg tatatcgagg gtgatgaaca
    27361 taaccctcat tccgcgtttc gtagcgcgcc atttgccccg gcggcacgcc cgcagatgaa
    27421 aatcaccggc attaaagcgc ctgaatttac ccccagccag actgaagaag gcgacctgca
    27481 ttacggtcat ggcgaagcgg gcactcacac accgggcatg agccagcgtc ggatgctgga
    27541 agtcgaaatt gacgtgctgg acaaaaacaa ccgtctggct gaacgcaacc gcgcgcgctt
    27601 tgctgcccgc aagcaactgg tgctcaacct ggtttccagc cctggttccg gtaaaaccac
    27661 cctgctgacg gaaaccttaa tgcgcctgaa agacagcgtt ccgtgcgcgg ttattgaagg
    27721 cgaccagcaa accgtgaacg atgccgcacg cattcgcgct accggtacac cagcgattca
    27781 ggtgaacacc ggtaaaggct gccatcttga cgcacagatg attgccgacg ccgcaccgcg
    27841 tctgccactg gacgataacg gtattctgtt tatcgaaaac gttggcaacc tcgtatgccc
    27901 ggccagcttc gatctcggtg aaaaacacaa agtagcggtg ctttccgtta ccgaaggtga
    27961 agacaaaccg ctgaaatatc cgcatatgtt tgccgccgcc tcgctgatgc tgctcaacaa
    28021 agttgacctg ttgccgtatc tcaactttga cgttgagaag tgcatcgcct gcgcccgcga
    28081 agtcaatcca gaaattgaaa tcatccttat ttccgccacc agcggcgaag ggatggacca
    28141 gtggctgaac tggctggaga cacagcgatg tgcataggcg ttcccggcca gatccgcacc
    28201 attgacggca accaggcgaa agtcgacgtc tgcggcattc agcgcgatgt cgatttaacg
    28261 ttagtcggca gctgcgatga aaacggtcag ccgcgcgtgg gccagtgggt actggttcac
    28321 gttggctttg ccatgagcgt aattaatgaa gccgaagcac gcgacactct cgacgcctta
    28381 caaaacatgt ttgacgttga gccggatgtc ggcgcgctgt tgtatggcga ggaaaaataa
    28441 tgcgttttgt tgatgaatat cgcgcgccgg aacaggtgat gcagttaatt gagcatctgc
    28501 gcgaacgtgc ttcacatctc tcttacaccg ccgaacgccc tctgcggatt atggaagtgt
    28561 gtggcggtca tacccacgcc atttttaaat tcggcctcga ccagttactg cctgaaaacg
    28621 ttgagtttat ccacggtccg ggttgcccgg tgtgcgtact gccgatgggt agaatcgaca
    28681 cctgcgtgga gattgccagc catccggaag tcatcttctg tacctttggc gacgccatgc
    28741 gcgtaccagg gaaacaggga tcgctgttgc aggcaaaagc acgcggtgcc gatgtgcgca
    28801 tcgtttactc gccgatggat gcgttgaaac tggcgcagga gaatccaacc cgcaaagtgg
    28861 tgttcttcgg cttaggtttt gaaaccacca tgccgaccac cgccatcact ctgcaacagg
    28921 cgaaagcgcg tgatgtgcag aatttttact tcttctgcca gcacattacg cttatcccga
    28981 cgctgcgcag tttgctggaa cagccggata acggtatcga cgcgttcctc gcgccgggcc
    29041 acgttagtat ggttatcggc actgatgcct ataattttat cgccagcgat tttcatcgtc
    29101 cgctggtggt cgctggtttc gaaccccttg atctactgca aggcgtggtc atgctggtgg
    29161 agcagaaaat agcggcccac agcaaggtag agaatcagta tcgtcgggtg gtaccggatg
    29221 ccggtaacct actggcgcaa caggcgattg ccgatgtgtt ctgtgtcaac ggcgacagcg
    29281 aatggcgcgg cttaggcgtg attgaatctt ctggcgtgca cctgacgccg gattatcaac
    29341 gattcgatgc cgaagcacat ttccgcccgg caccgcagca ggtctgcgat gacccgcgcg
    29401 cgcgttgtgg tgaggtatta acgggcaaat gtaagccgca tcaatgcccg ctgtttggta
    29461 acacctgtaa tcctcaaacc gcatttggtg cactgatggt ctcctccgaa ggagcgtgcg
    29521 ccgcgtggta tcagtatcgt cagcaggaga gtgaagcgtg aataatatcc aactcgccca
    29581 cggcagcggc ggccaggcga tgcagcaatt aatcaacagc ctgtttatgg aagcctttgc
    29641 caacccgtgg ctggcagagc aggaagatca ggcacgtctt gatctggcgc agctggtagc
    29701 ggaaggcgac cgtctggcgt tctccaccga cagttacgtc attgacccgc tgttctttcc
    29761 tggcggtaat atcggcaagc tggcgatttg cggcaccgcg aatgacgttg cgggcagtgg
    29821 cgctattccg cgctatctct cctgtggctt tatcctcgaa gaaggattgc cgatggagac
    29881 actgaaagcc gtagtgacca gcatggcaga aaccgcccgc acggcaggca ttgccatcgt
    29941 tactggcgat actaaagtgg tgcagcgcgg cgcggcagat aaactgttta tcaacaccgc
    30001 tggcatgggc gcaattccgg cgaatattca ctggggcgca cagacgctaa ccgcaggcga
    30061 tgtattgctg gttagcggta cactcggcga ccacggggcg actatcctta acctgcgtga
    30121 gcagctgggg ctggatggcg aactggtcag cgactgcgcg gtgctgacgc cgcttattca
    30181 gacgctgcgt gacattcccg gcgtgaaagc gctgcgtgat gccacccgtg gtggtgtaaa
    30241 cgcggtggtt catgagttcg cggcagcctg cggttgtggt attgaaattt ctgaatcagc
    30301 gctgccggtt aaacctgccg tgcgcggcgt ttgcgaattg ctgggactgg acgccctgaa
    30361 ctttgccaac gaaggcaaac tggtgatcgc cgttgaacgc aacgcggcag agcaagtgct
    30421 ggcagcgtta cattcccatc cactggggaa agacgcggcg ctgattggtg aagtggtgga
    30481 acgtaaaggt gttcgtcttg ccggtctgta tggcgtgaaa cgaaccctcg atttaccaca
    30541 cgccgaaccg cttccgcgta tatgctaata aaattctaaa tctcctatag ttagtcaatg
    30601 accttttgca ccgctttgcg gtgctttcct ggaagaacaa aatgtcatat acaccgatga
    30661 gtgatctcgg acagcaaggg ttgttcgaca tcactcggac actattgcag cagcccgatc
    30721 tggcctcgct gtgtgaggct ctttcgcaac tggtaaagcg ttctgcgctc gccgacaacg
    30781 cggctattgt gttgtggcaa gcgcagactc aacgtgcgtc ttattacgcg tcgcgtgaaa
    30841 aagacacccc cattaaatat gaagacgaaa ctgttctggc acacggtccg gtacgcagca
    30901 ttttgtcgcg ccctgatacg ctgcattgca gttacgaaga attttgtgaa acctggccgc
    30961 agctggccgc aggtgggcta taccccaaat ttggtcacta ttgcctgatg ccactggcgg
    31021 cgggagggca tatttttggt ggctgtgaat ttattcgtta tgacgagcgt ccctggagcg
    31081 aaaaagagtt caatcgtctg caaacattta cgcagatcgt ttctgtcgtc accgaacaaa
    31141 tccagagccg cgtcgtcaac aatgtcgact atgagttgtt atgccgggag cgcgataact
    31201 tccgcatcct ggttgccatt accaacgcgg tgctttcccg cctggatatg gacgaactgg
    31261 tcagcgaagt cgccaaagaa atccattact atttcgacat tgacgatatc agcattgtct
    31321 tacgcagcca ccgtaaaaac aaactcaaca tctactccac tcactatctt gataaacaac
    31381 atcccgccca cgaacagagc gaagtcgatg aagccggaac cctcaccgaa cgcgtgttca
    31441 aaagtaaaga gatgctgctg atcaatctct acgagcggga cgatttagcc ccctatgaac
    31501 gcatgttgtt cgacacctgg ggcaaccaga ttcaaacctt gtgcctgtta ccgctgatgt
    31561 ctggcgacac catgctgggc gtgctgaaac tggcgcaatg cgaagagaaa gtgtttacca
    31621 ctaccaatct gaatttgctg cgccagattg ccgaacgtgt ggcaatcgct gtcgataacg
    31681 ccctcgccta tcaggaaatc catcgtctga aagaacggct ggttgatgaa aacctcgccc
    31741 tgaccgagca gctcaacaat gttgatagtg aatttggcga gattattggc cgcagcgaag
    31801 ccatgtacag cgtgcttaaa caagttgaaa tggtggcgca aagtgacagt accgtgctga
    31861 tcctcggtga aactggctcg ggtaaagagc tgattgcccg tgcgatccat aatctcagtg
    31921 ggcgtaataa tcgccgcatg gtcaaaatga actgcgcggc gatgcctgcc ggattgctgg
    31981 aaagcgatct gtttggtcat gagcgtgggg cttttaccgg tgccaacgcc cagcgcattg
    32041 gtcgttttga actggcggat aaaagctctc tgttcctcga cgaagtgggc gatatgccac
    32101 tggagttaca gccgaagttg ctgcgtgtat tgcaggaaca ggagtttgaa cgtctcggca
    32161 gcaacaaaat cattcagacg gacgtacgtt taattgccgc gactaaccgc gatctgaaaa
    32221 aaatggtcgc cgaccgagag ttccgtagcg atctctatta ccgcctgaac gtattcccga
    32281 ttcacctgcc gccactacgc gagcgtccgg aagatattcc gctgctggcg aaagccttta
    32341 ccttcaaaat tgcccgtcgt ctggggcgca atatcgacag cattcctgcc gagacgctgc
    32401 gcaccttgag caacatggag tggccgggta acgtacgcga actggaaaac gtcattgagc
    32461 gcgcagtatt gctaacacgc ggcaacgtgc tgcagctgtc attgccagat attgctttac
    32521 cggagcctga aacgccgcct gccgcaacgg ttgtcgccca ggagggcgaa gatgaatatc
    32581 agttgattgt gcgcgtgctg aaagaaacca acagcgtggt tgccgggcct aaaggcgctg
    32641 cgcaacgtct ggggctgaaa cgcacgaccc tgctgtcacg gatgaagcgg ctgggaattg
    32701 ataaatcggc attgatttaa ctgcaaattg ccggacagat ctgcctgtcc ggcatactat
    32761 tcacgaggtt ttttcggacg atatttttcc ggcagttctg gcaccggacg cttgtcatcg
    32821 atgagatgac gcacggttaa gatcggatga cgccacagca ttctcggccc ggcccaacgc
    32881 ataatctgtt tcatctcttc acgctttgca ggctggtaac agtgcaccgg acactgctta
    32941 caggctggtt tctcttcgcc gaacacacat ttatccagcc gcttttgcgc gtaaacaaac
    33001 aacgcctcgt aatgctccgg ctccgctgac gcctgcgggc atttcgcttg ataaagatcg
    33061 atcatttttt taatcgtcag tttttcacga gagatacgct tgccggacat gctgcctcca
    33121 cctcattaag atgcatttat attacatctt aat
//
LOCUS       DAJTSA010000037        31550 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000037 DAJTSA010000000
VERSION     DAJTSA010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.037"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="OO369_001896"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="OO369_001896"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR5982193.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="OO369_001897"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="OO369_001897"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR5982194.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO369_001898"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO369_001898"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR5982195.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO369_001899"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO369_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR5982196.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO369_001900"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO369_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR5982197.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO369_001901"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO369_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR5982198.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="OO369_001902"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="OO369_001902"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR5982199.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="OO369_001903"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="OO369_001903"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR5982200.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="OO369_001904"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="OO369_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR5982201.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO369_001905"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO369_001905"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR5982202.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO369_001906"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO369_001906"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR5982203.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="OO369_001907"
     CDS             8212..9075
                     /locus_tag="OO369_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR5982204.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="OO369_001908"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="OO369_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR5982205.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="OO369_001909"
     CDS             10411..11028
                     /locus_tag="OO369_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR5982206.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO369_001910"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO369_001910"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR5982207.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="OO369_001911"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="OO369_001911"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR5982208.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO369_001912"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO369_001912"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR5982209.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="OO369_001913"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="OO369_001913"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR5982210.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="OO369_001914"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="OO369_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR5982211.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="OO369_001915"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="OO369_001915"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR5982212.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO369_001916"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO369_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR5982213.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="OO369_001917"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="OO369_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR5982214.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="OO369_001918"
     CDS             21859..23568
                     /locus_tag="OO369_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5982215.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO369_001919"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO369_001919"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR5982216.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="OO369_001920"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="OO369_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="OO369_001921"
     tRNA            27625..27719
                     /locus_tag="OO369_001921"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            27991..29202
                     /locus_tag="OO369_001922"
     CDS             27991..29202
                     /locus_tag="OO369_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5982217.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            29614..30657
                     /locus_tag="OO369_001923"
     CDS             29614..30657
                     /locus_tag="OO369_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR5982218.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31172..>31550
                     /locus_tag="OO369_001924"
     CDS             31172..>31550
                     /locus_tag="OO369_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HCR5982219.1"
                     /translation="MDENALRFASYWRNSLADAESGKGCFERKDAQNFTHWHGIAAGR
                     LDEAIVNKFFKGEKDDVETVNVILRPKVYFRLLQHGKDRSAGTPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGA"
BASE COUNT         7906 a         8319 c         7825 g         7500 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatgattt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcagcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtactgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cgaatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtaggtg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat
    28021 ggcattaacg atatcaaagt gaaaaccgca aaacccaaag acaaacccta taagcttgct
    28081 gatggcggcg gtatgtatct actgattaac actaatggtt ctaaatactg gcgcatgaag
    28141 taccgctttg ctggtaaaga aaagatgctt tctattggcg tataccccga tgtaacgcta
    28201 gccgatgctc gcgagaaacg cagtgaagcc aggaaactcc tcgctgctgg aggcgatcca
    28261 ggggaggcaa aaaaggaaga aaaaatcgcg cagcaaatga gtctgaaaaa tacgttcgag
    28321 gctattgctc gcgaatggca ccagttaaaa gctgatcggt ggtcattacg ctatcgtgat
    28381 gaaatcattg atacctttga aaaagacatt tttccctaca ttggcaagcg ccctattgcc
    28441 gaaatcaaac caatgaagtt actggaagca ctgcgtaaaa tggaaaaacg tggagctctg
    28501 gagaaaatgc gcaaggtccg ccagcgctgt ggtgaggtgt tccgctatgc aattgttact
    28561 ggccgggcag actataaccc agcccccgat cttgccagtg ctttagctac accgaaaaaa
    28621 gtacattttc cctttcttac tgctaatgaa cttccccact tcctcaacga tctggcgggt
    28681 tataccggaa gtataatcac taagacagct actcagatca ttatgttaac cggcgtacga
    28741 acacaagaac tgcgttttgc acgctgggaa gatatcgatt ttgagacaaa attgtgggag
    28801 atcccggcag aagtaatgaa gatgaagcga cctcatattg tgccgctctc tgagcaggtc
    28861 attatgctat ttaagcagct tgaaccaatc tcaaaacatc atcccctagt ctttatcggt
    28921 agaaatgatc cccgcaaacc aattagtaag gaaagcatta accaagtaat tgaattactt
    28981 ggatataagg gaagactcac agggcacggt ttcagacaca ccatgagtac aattctgcat
    29041 gaacaaggct ttaattccgc ctggattgaa atgcagttag ctcatgtgga taaaaattcc
    29101 atcaggggta cctataatca tgccctgtat ctcgatggcc gccgtgaaat gatgcaatgg
    29161 tacgctgatt acattgattc actctccagc cgggaaagtt aagtcagaaa tacttttccc
    29221 tgatgttttc ctcaattaag tattttatcc tctggcctac aaggctggag gatttttgtt
    29281 attatattat ttttttgcgt cttcaaactt attacaaaaa cataattaaa aacaaattga
    29341 catttttaaa ttggcaatta caaaatacag ccttgcagtt atttcgatac ctttagcaaa
    29401 ggaatagttg attcagatat aatttagcat ttcaatcatc tgacatcatt ataaaaaatg
    29461 agattcacat aaagataaaa ttattgttct caaggaaagc atgaaggggc ttcgtaagca
    29521 atattcattc ggtgccaaga caggacttga tacacagaat ctcaactttt atgctctcag
    29581 actatatcac cacatgtttt acaggctcag aaaatgaacg atagattatg ctttgaagtg
    29641 cacgataaca agggttattt cgtatttccg gatacctggt ttggcccatt gctgggggag
    29701 tttgaagaag tgcttgatgc atacgacgct gatgaaatca gcgagacgag ttacatcaac
    29761 aaactccgac gccttgctca acgggagccg gactttattg atatacacgc tcatctggca
    29821 tatgcatttc tggaacagaa tgccccgcgt aaggcactga acgccgctct taaaggactg
    29881 gcggcaggta atcgtatcat cccggaaagt ttctgtggtg aaataatctg gatgcatccc
    29941 gaaaaccggc cttatctgcg cgcgctttat gcagcaattc tcgcaaatgt gcacttacag
    30001 cgccatcagg atgcggttat gctcactgat aaaatactgg catacaatcc tgaagataat
    30061 cagggggctc gctggttgtt gggttcagag ctgttacgca ccggcgatca tgagcgagcc
    30121 ttcagtgtac tgaaagagca tgctgatgaa ttttcacctt actggtatga actcggtctc
    30181 ctgcattttc tgaacgggga acatgttaag gccgccacag cttttcgcca tggctttgcc
    30241 acaaacactt acatcgcaga aatgctgtgc ggaaatctgc atccatttcc acttgctgtg
    30301 tggcatgact tctccggctc tctggatacg gcagaagatt actatgcaac atactcacca
    30361 ttatggggac aatatccgga agcattgctg tttgtaaact ggctctataa ccattcctcc
    30421 gtgctgcatg aacgatcaga aatcattaaa tgcgccgaaa tgctgattca ggaagatgat
    30481 ttcgaaatat gcgaaagtat tttaagacag caggagcacc tgtggaaaag aattgacaag
    30541 acactttctg aggaaattgt acagaaatgc agaaatatga acggtgaata tatctggccc
    30601 tggatattgc ccttttccgc agcagggatg aaacattcaa gcatccagta tcagtagata
    30661 ttgcattagt gtaactttaa aacaagtaat aatttattat ccaataataa cactgtgttc
    30721 cttccccagg tgattaccct ggggttattt tctgaaagtt tcctttgcaa aaggatagcg
    30781 ttgtaaatta ctttaacgaa ttactccatg aactcattaa agttctttcg taatacttta
    30841 tcgtcaaaca cgttatagct ggcatcaaaa accacagatt taccctgcca gcgggtaatc
    30901 ccctcctctg gcgttttcgg ttgaactggt atccccgtcc aacacaggca gccattataa
    30961 acagtaaaaa attgtattcg ctgttatgaa atcaatagtc ctgacgtact gttgaatgtg
    31021 cttgtgttct gaacctcgta caaccagtac atttgatatc gaattataaa caaaacattg
    31081 catccaactt ttttaatatg gacaatagtt gtccatacgg tggttttata ttcgataaat
    31141 caatggattt cattgagtca caggactggt aatggatgaa aatgctttaa ggtttgcctc
    31201 atactggcgc aactcgcttg cagatgctga gtcaggaaag ggctgttttg aacggaaaga
    31261 cgcccaaaat ttcactcact ggcatgggat agcggcggga cgtcttgacg aagcgattgt
    31321 caataaattt tttaagggag aaaaagacga tgtcgaaact gtcaatgtca tcttacgccc
    31381 aaaagtttat ttccggttac tgcagcatgg taaggaccgt tccgcaggca cgcctgatat
    31441 tgttacaccg atagtgacgc cagccttgct aagccgtgag ggttttttat atccgacgcc
    31501 agcgacctcc attcccagag acctgcttga acctttgcca aaaggagcat
//
LOCUS       DAJTSA010000038        31424 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000038 DAJTSA010000000
VERSION     DAJTSA010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.038"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..441)
                     /locus_tag="OO369_001925"
                     /pseudo
     CDS             complement(1..441)
                     /locus_tag="OO369_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(416..1216)
                     /gene="otsB"
                     /locus_tag="OO369_001926"
     CDS             complement(416..1216)
                     /gene="otsB"
                     /locus_tag="OO369_001926"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR5982220.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(1383..2369)
                     /gene="araH"
                     /locus_tag="OO369_001927"
     CDS             complement(1383..2369)
                     /gene="araH"
                     /locus_tag="OO369_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR5982221.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2384..3898)
                     /gene="araG"
                     /locus_tag="OO369_001928"
     CDS             complement(2384..3898)
                     /gene="araG"
                     /locus_tag="OO369_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR5982222.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(3968..4957)
                     /gene="araF"
                     /locus_tag="OO369_001929"
                     /pseudo
     CDS             complement(3968..4957)
                     /gene="araF"
                     /locus_tag="OO369_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            5754..6257
                     /locus_tag="OO369_001930"
     CDS             5754..6257
                     /locus_tag="OO369_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR5982223.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6337..6588)
                     /locus_tag="OO369_001931"
     CDS             complement(6337..6588)
                     /locus_tag="OO369_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR5982224.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="OO369_001932"
     CDS             complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="OO369_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR5982225.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7052..7375
                     /locus_tag="OO369_001933"
     CDS             7052..7375
                     /locus_tag="OO369_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR5982226.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7546..8043
                     /gene="ftnA"
                     /locus_tag="OO369_001934"
     CDS             7546..8043
                     /gene="ftnA"
                     /locus_tag="OO369_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR5982227.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8080..8319)
                     /locus_tag="OO369_001935"
     CDS             complement(8080..8319)
                     /locus_tag="OO369_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR5982228.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            8509..9720
                     /gene="tyrP"
                     /locus_tag="OO369_001936"
     CDS             8509..9720
                     /gene="tyrP"
                     /locus_tag="OO369_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR5982229.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="OO369_001937"
     CDS             complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="OO369_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR5982230.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10643..10729)
                     /locus_tag="OO369_001938"
     tRNA            complement(10643..10729)
                     /locus_tag="OO369_001938"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10693..10695),aa:Leu,seq:taa)
     gene            complement(10742..10815)
                     /locus_tag="OO369_001939"
     tRNA            complement(10742..10815)
                     /locus_tag="OO369_001939"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10781..10783),aa:Cys,seq:gca)
     gene            complement(10870..10945)
                     /locus_tag="OO369_001940"
     tRNA            complement(10870..10945)
                     /locus_tag="OO369_001940"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10910..10912),aa:Gly,seq:gcc)
     gene            complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="OO369_001941"
     CDS             complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="OO369_001941"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR5982231.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="OO369_001942"
     CDS             complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="OO369_001942"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR5982232.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="OO369_001943"
     CDS             complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="OO369_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR5982233.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14483..14659
                     /locus_tag="OO369_001944"
     CDS             14483..14659
                     /locus_tag="OO369_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5982234.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            14646..14870
                     /locus_tag="OO369_001945"
     CDS             14646..14870
                     /locus_tag="OO369_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR5982235.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="OO369_001946"
                     /pseudo
     CDS             complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="OO369_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(15753..16505)
                     /gene="yecC"
                     /locus_tag="OO369_001947"
     CDS             complement(15753..16505)
                     /gene="yecC"
                     /locus_tag="OO369_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR5982236.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="OO369_001948"
     CDS             complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="OO369_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR5982237.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="OO369_001949"
     CDS             complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="OO369_001949"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR5982238.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="OO369_001950"
     CDS             complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="OO369_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5982239.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="OO369_001951"
     CDS             complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="OO369_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR5982240.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(19761..20480)
                     /gene="fliA"
                     /locus_tag="OO369_001952"
                     /pseudo
     CDS             complement(19761..20480)
                     /gene="fliA"
                     /locus_tag="OO369_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(20645..22297)
                     /gene="fliC"
                     /locus_tag="OO369_001953"
     CDS             complement(20645..22297)
                     /gene="fliC"
                     /locus_tag="OO369_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR5982241.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMLKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            22563..23969
                     /gene="fliD"
                     /locus_tag="OO369_001954"
     CDS             22563..23969
                     /gene="fliD"
                     /locus_tag="OO369_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR5982242.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            23994..24404
                     /gene="fliS"
                     /locus_tag="OO369_001955"
     CDS             23994..24404
                     /gene="fliS"
                     /locus_tag="OO369_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR5982243.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            24404..24769
                     /gene="fliT"
                     /locus_tag="OO369_001956"
     CDS             24404..24769
                     /gene="fliT"
                     /locus_tag="OO369_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR5982244.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            24847..26334
                     /gene="amyA"
                     /locus_tag="OO369_001957"
     CDS             24847..26334
                     /gene="amyA"
                     /locus_tag="OO369_001957"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5982245.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(26368..26781)
                     /locus_tag="OO369_001958"
     CDS             complement(26368..26781)
                     /locus_tag="OO369_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5982246.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            26968..28173
                     /gene="yedE"
                     /locus_tag="OO369_001959"
     CDS             26968..28173
                     /gene="yedE"
                     /locus_tag="OO369_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR5982247.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            28170..28403
                     /gene="yedF"
                     /locus_tag="OO369_001960"
     CDS             28170..28403
                     /gene="yedF"
                     /locus_tag="OO369_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR5982248.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            28510..29180
                     /locus_tag="OO369_001961"
                     /pseudo
     CDS             28510..29180
                     /locus_tag="OO369_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(29220..30428)
                     /locus_tag="OO369_001962"
                     /pseudo
     CDS             complement(29220..30428)
                     /locus_tag="OO369_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            30496..31111
                     /gene="tnpA"
                     /locus_tag="OO369_001963"
                     /pseudo
     CDS             30496..31111
                     /gene="tnpA"
                     /locus_tag="OO369_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         7868 a         8066 c         7427 g         8063 t
ORIGIN      
        1 caatgcgatt attcacccca cgtttgcgta attcatgcgc aaagggcaac aggtgataat
       61 cgtggatcca gataatgtca tcgtcttgca acagcggcag taatttatct gccagcaacg
      121 catttacgcg tagatagccg tcccaggcag gacgctgaaa ttgcaccaga tcgagccgat
      181 aatgaaaagc gggccagaga acggcattgg agaattggtt gtagtattcg tcaaggtcct
      241 gttcgctgag gttaaaagag gcccacgtaa tgttaccttt tttagcggct gatcctcatt
      301 ccctgtttca ccactccagc caaaccacag tccgcctgcg gctttcagtg cccccagtat
      361 gccaacggca aggccaccgg cactggcggc gtgctcgtct ggtggtgcaa tccggttaga
      421 tactacgact aaacgactca tagtcatcac tcctgttatt ttctcttttt tgttgtaatg
      481 cggcggttat catttcaagc cagctccaga catccggcac acccgccagt cgccatgatg
      541 cctgagttgc acctgtgcca atttttactg acattccgcc cagtcggtta acgactgcga
      601 agccagattc atcggttaaa tcatcgccca gaaatacggg cgttcgcccg ataaaaggag
      661 cttcctgcat aaaggctgca atcgcttcac ctttactggt acctctcggt ttgatctcga
      721 caacacactt tccctgctgt aacgccattt gtggccagat ctgagtaata cgttgcgcta
      781 atgtcattaa tgcgtcttca tgctgcggag cctgacgata atgcagcgca aaagccatcc
      841 ctttcgcctc cagctccgcg ccgggatact gagcgatgac cgtatgcagt tgcacgctaa
      901 tatcacgcgc aatcgcatcc ggcagatgaa cgatatgtgt tttaccattg atgtcacggc
      961 gctccgcccc atgcacgccc gctaacggga agcgataagg ttttgccagt gcgtcaagct
     1021 ccaccattga gcgccctgat atcaatgcca atgcaccatc acttgcggtt gccagtagct
     1081 gtagtccttg cagaatattg tcaggcacga cgacctgatc gggatgcggt ttgatttccg
     1141 ccagcgttcc atcaagatca aaaaaccagg catatttcgc ggatagttca ggggtttcgg
     1201 ttaacggttc tgtcacccgg ctctcctcct tctcttttct tattgtgttg cgcagaccgc
     1261 gctttacaga catgtaagta tagacagtgt gacggaggtc gccatttgca acgaagaaca
     1321 gccaaccgtt gtggcggctg gctgtggtgg gaaaaaacgt taagttgttg tggaaaaaag
     1381 catcagacag tgcgcttcgc tttttgcttg taacggtcga agatcaccgc tgccagcagg
     1441 attaagccgc gaaccacgta ctgcgcgaaa ggagaaatat taagcaggtt catggcgttt
     1501 tccacggtgc ctaaaattaa gatacccgcc accacatatg agatttttcc gatgccacct
     1561 ttcagagaaa cgccacctaa aacgcaggcg gagataacaa taagctcata accaatcgac
     1621 gtcattggct gcccactagt catacgtgaa gccagaataa ttccggctat cgcagatacc
     1681 aggccagaga gaacaaagat aataattttg gtgcgaacaa ccggtacacc cgccagacgc
     1741 gcagcctctt cgttcccgcc aatcgccagg gtattacgac caaaggtggt tttattcagc
     1801 agcaaaccaa agataatcag acacgcgacg gtgagccaga ttggcgcagg cagaccgaac
     1861 cagttagcgt aaccaagggc aaagaagctt tcatcttcga taccgaccgc tttaccgtct
     1921 gaaatgatgt acgccagacc tcgaacaatc tgcatcgttg ccagtgtcgt gatcagagca
     1981 tttattttca gtttggcgat aacaaagcca ttgaccaggc cacagagaac gcccagcaac
     2041 aaacccgctg ccacgccaat ccacaggctt tcagtcaggt tgataaccac cgccgtggtg
     2101 acacccgcac aggcaattac ggaggcgaca gaaaggtcaa agtcaccgga agcaaggcag
     2161 aacaacatgc cacacgccac catccccgac atggaaattg ccaggcccaa ccctttcata
     2221 ttaatgaagg tggcaaaatt tgggacaaaa atggcacagg cgataaagag caccgcaaac
     2281 accaccagca tgccgtactg atcccagata cgcccgaagc tgaatgacga cttaggtgcg
     2341 ccagaccccg atgtagaaac agaagacatc atactctcct tactcaggca actgcctggc
     2401 tgactttagg catcgcaagg ctcagtgcct gacgctcatc tgcctgctcg tgtaacaatt
     2461 caccggcgat ttcgccttcc cgcatcacca caatccggtc ggcaacgccg aggacttcag
     2521 gtaagtcgct ggaggcaaac agcaccgcca cgccctgcgc cgccagcgca taaattacgt
     2581 tatatatttc gtgcttagcg ccaacatcaa tgccgcgcgt aggttcatcc agcaaaatga
     2641 ccttcatctc ttccgataac cagcggccca gaatggcttt ttgctgattt ccgcctgaga
     2701 gattcatgat cagttgctcc gcgcccggcg ttttgatgtt gagcgaacga atgtgctgat
     2761 cggcattgtt ttcttcccaa ctgttgttga ttacacaacc gccgagcaca tgtttgcgtc
     2821 tggcactgat gttgatattg tcgcgaacgg agtgcacggg aataatgcct tccgctttgc
     2881 gagcttccgg gcagagcatc atgcctgcgg caatggcgtg gctcggttta cgaatatcga
     2941 tcggctgttg gtcgatataa acctgaccgg cggtgatttg cgtcccgcca aacaagcctt
     3001 tcattaattc gctacgcccc gcccctacca gaccaaacag cccaacaatt tcaccactgc
     3061 gaaccgccag acttattggc gtacgcacgc ctagtgcttt cacagcatca agacgcaggc
     3121 gctcctcgcc ataactacga ggttgccagc cgtagatatc gccaatgtcg cgcccgacca
     3181 tcgcctgcac cagcgcgtcg tggtcaacct gctgcatatc ggtaaaggtt ttgacataac
     3241 gtccatcttt aaagacagtg atggcatcgc tgagggcaaa tatttcttcc atacggtgag
     3301 aaacgtataa gatcacccgc ccctcttttc gcagttcacg aataacgcgg aaaagattgc
     3361 cgatttcgcg ggcagaaagg gagctggttg gctcatcaaa ggcgataatt ttagcgttac
     3421 gcgccagcgc tttggcgatt tcaaccatct gccactgacc aatggcgaga tatttcagcg
     3481 gcgtgtccgg gtcaatatcc ataccaagat gtttaagttg taaacccgcc tcataattca
     3541 gcaatgagcg attcacaatg ccgcctttat gcggcagctg gccgagatag atgttttccg
     3601 cgacggtcat ttccggcacg agatgcagtt cctggtaaat aatcgccaca cccgcgttaa
     3661 gtgctgcggt cgtgtcggaa aaggacattt cctgcccatt aatcactaca gaacccgtgg
     3721 ttggcgcata gttgccgctg aggattttta agagagttga ttttcctgcg ccattttcac
     3781 ccatcaacgc atgaacctga ccggcatagc agtcaaaact aatatccgtc agcgccttaa
     3841 cgccgggaaa cgttttaccg atgccgcgaa atgagagata cggggtagac tgttgcataa
     3901 cgtctccgtg aatcactggt cgttcacacc ccctctgcat catgcagagg ggaatttttc
     3961 cggcaaatta cttaccgcct aaaccttttt tctccagttc ttctttaaag ttgtcacgcg
     4021 tgatcagtac cacgtctgta acttcggtaa attttggcgt ttcaacgtct tttgctaccc
     4081 agttgtaaag catttcgctg gatttatagc catgtacgtc cgggcttggc agcagggaac
     4141 cgtagaagcc ggttgcctgt gctttagaca gttcgcttac tgcatccaca ccgttaatac
     4201 caataccgat gatatcggct gctttaaagc cctgaccttc ggttgcacgt acgccgccaa
     4261 gtacggtgct gtcgttcata ccgacgatca gccaatgttt aacttccgga tgttgaacca
     4321 gcattgagtt ggcagcgtca aatgcccccg ggatgtcgtt agatttggta ggtacctgat
     4381 aaatttgttt ttccgggaat cctgcagctt tcagtgcgtc catagaaccc gttgtacggc
     4441 ggcgggcggt atccagttcg ttggcggtaa tcgccatcac tgcgctttct ttgacatccc
     4501 agccacgttt ctgcatctct ttatacagtt cctagccctg acgttcgcca attttagtcg
     4561 ccgccatcat caccagagga acggtatcca ttggcttacc tttggcgtta acaaactggt
     4621 catccactgc aatgactttc atatcgtagc cacgcgcttt cgcgacgatg gcagagccga
     4681 gtttcgggtc cggagtacaa ataacgaaac cttttgcgcc actggctgcc aggctgtcga
     4741 tcgcgttcaa tgttttttcg ccatccggca cggcaatctt aataacctca aaccctaaat
     4801 ccttcccggc tttatcggca aacttccatt cggtctggaa ccacggctct tccggttgct
     4861 tcaccagaaa accgagcttc aggttctccg ccatagcgga ttgtgacata acggctgcca
     4921 gaccaatggc tgccagggct ttagtaaatt tgtgcatggt tctctccagc tttagtgtcg
     4981 ttttgtgtag ggcaaaaacg aatgacattc gttaaattaa tcggaaaaca aagcattacc
     5041 ttttatctaa aagataagtg accgtgttga catagtttta gcgagaaatt aattctccat
     5101 aggagagcaa tatcacatcg cagaattaca gtgagaacgt gcataaattt agcgggaaaa
     5161 gacataagga aaagccaatt tgtcagacaa attgttgaat gcacagcaga ttaatccata
     5221 agattagcct ggaaatcctt gttgtcttcg gtacccatgc gggatgtctt ctttttaacc
     5281 agtcaatagg ccgcattacc tggcgttgag tttttgaaat ggtgtaataa ccacaactca
     5341 aagatgtgga aaatgcgcgt cattcatttc gccattaatt atcactgtgc tcattaatta
     5401 acagaacacg tataatgaga gccatctcgc aaaaatgaaa aaacgtttta taaaatcatc
     5461 acttcatcat gaattcaaat tcatcgatta atatcaacaa gatacaaaaa gcactatcat
     5521 taaaattcat tgcatttaca ttgatttcac caatgaaatg taaaaatata taaacttgat
     5581 gatttaagca ttttcttata accgttcaga cgttattctt atttcagatc atcgtcagaa
     5641 ttgactccac gatcacattt cggaccggca gaaaggaatt attctgcaaa cagtaattat
     5701 ggtgttttga tttatcttgc acctctccac ttctggatat aaggatatta ggtatggcaa
     5761 ccgctggaat gcttctcaaa ctcaactctc aaatgaaccg cgagttttac gcatccaatc
     5821 tctaccttca cctgagtaac tggtgttctg aacagagtct gaacggcacc gccactttcc
     5881 ttcgcgccca ggcacagagt aatgtgaccc aaatgatgcg catgtttaac tttatgaaga
     5941 gtgtcggcgc tacccccatc gttaaagcca ttgatgttcc aggtgaaaaa ctgaactctc
     6001 tggaagaact gttccaaaaa acgatggaag aatacgagca acgttcaagc actctggcgc
     6061 agttagccga tgaagcgaaa gaactgaatg atgattcaac cgtcaatttc ctgcgcgatc
     6121 tggaaaaaga acagcagcat gatggcctgt tgctgcaaac cattcttgat gaagtgcgca
     6181 gtgcgaaact tgcgggtatg tgccctgtgc agaccgacca acatgttctg aatgtcgtgt
     6241 cacaccagct gcattgatca tacatcggcg ctaatacatt gcgccgatga agtttttgag
     6301 aaaccgctgc ctcatctgtt tgaagcagcg gtttttttaa tgggattcac cctgtggggt
     6361 aaacttgagt tcaatcagcg cgatggcttt ttggattgcc cgcatggtga ccgggtctgc
     6421 ggcagcggga tgatgagtaa agtcgatatt cttcagctga ctggacattt tttcacgaac
     6481 ttcaacgggc gcgattacat cgagaacatc cagaatttgt ttgataacca actggcaagc
     6541 taccacatca gaaaccagtt cctgatcggc attcagcggc tgggacatcg taaactcctg
     6601 atagcatttt gaaagccgtt atagtagcgg cttcacatct tcagcgatag tcacatccac
     6661 cgtcatcagg acacaaaaaa acctgccgga gcaggttttt tgttatcgga acatattgcc
     6721 tggcggtacg tctttgaacg tcttgcaata gttattgaac atacttttca ggattttgcg
     6781 cagtttcatc gcggcactcc gaccatttgt tatacaggtg ttattgtctt tgcgcttata
     6841 atatgacaac catcacaaaa atcaatcttt atgtgataca aatcacataa ataccccttt
     6901 aatgttataa aaatgataat caaaaaacag cccccctatt tctgacacct acagatggca
     6961 agaaatagcg cctgccaggc gtcttttccg gccattgtcg cagcgctgta acgcgtaaaa
     7021 tagtgctttc tcttactctt ctggctggac catgagactt ctgattctga ctctttcatt
     7081 aataacgctg gcggggtgta cggttactcg ccaggcccat gtgagcgaag ttgacgcggc
     7141 aacaggtatt gtacggttgg tttatgacca ggcttttttg cagcatgccc ataccgatcg
     7201 ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag gaaggctata cccacgcgat
     7261 cccctttggt cagccagtag gcaactgtag cctttttgcg ggttccttat gtttgaatac
     7321 ggaattcact ttgtcatatc agtgccatca ttccgccttc cctgtctttc tctaaatatt
     7381 ttaatcagcg aggggatctt cgctgattaa agaaatagcg gtaatgcgtt ttattcccat
     7441 tcgtattttt aataattgaa gtttatattt taccttttgc aaataataaa ataacaaatt
     7501 atagtgacgc cacagcaaca aatataacct ttgtggagca ctatcatgct gaaaccagaa
     7561 atgattgaaa aacttaatga gcagatgaac ctggaactgt actcttcact gctttatcag
     7621 caaatgagcg cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt cctgcgccgt
     7681 cacgcccagg aagagatgac gcatatgcag cgtctgtttg attacctgac tgataccggc
     7741 aatttaccgc gtattaatac cgttgaatct ccgtttgctg aatattcctc acttgatgaa
     7801 ttattccagg aaacctataa acacgaacaa ttaatcaccc agaaaattaa cgaactggct
     7861 catgctgcaa tgaccaatca ggactaccca acatttaatt tcctgcagtg gtatgtttct
     7921 gagcagcatg aagaagagaa actgttcaaa tcgattattg ataaattaag cctggcaggc
     7981 aaaagcggcg aaggtctgta ttttatcgac aaagaactct ctaccctcga cacacaaaac
     8041 taatgctctc cggcggcagg tttctgccgc cgtttcaaat cagtgacggc aaatcttact
     8101 ctgatcggtc gaaaaacctt cttctgaagg tataaatttg ccttttgctg ccagaaacgc
     8161 taccagctct cccgctgtca ccccttctgc cgagcacgtg tgaaaacgtg cctgttcacc
     8221 aaaacgcgct ttaatcgcag cttccagact ggcatgcgta tattgctcgc ctgattcaat
     8281 catcatattt aacacttcat gaccgtgaat agagtccatc gttcctcctc aaaaaaagcc
     8341 tagcgtagcg attaccgctt atgaagactt tgcgccagcg caggactgaa tgcttttatt
     8401 gtacatttat atttacacaa tatgtaacgt cggtttgacg aagcagccgt tatgccttaa
     8461 cctgcgccgc agatatcact cataaagatc gtcaggacag aagaaagcgt gaaaaacaga
     8521 accctgggaa gtgtttttat cgtggcggga accacaattg gcgcaggcat gctggcaatg
     8581 ccgctggctg cggccggtgt tggttttagc gttacgttaa tcttgttgat tgggctttgg
     8641 gcgttgatgt gctacacggc gctattactg ctggaggtgt accagcatgt tccggcagat
     8701 accggtctgg gcacgctggc aaaacgctat ctgggacgct acggtcaatg gctgacgggc
     8761 ttcagtatga tgttcttaat gtatgctctg actgcagcat acatcagcgg tgccggtgaa
     8821 ttgttggcct ccagcatcag cgactggaca ggtatttcaa tgtcggcaac cgctggcgtg
     8881 ctgttgttca cttttgttgc cggtggcgtg gtttgtgttg gaacatcgct ggtcgattta
     8941 tttaaccgtt ttctgttcag cgccaaaatt atttttctgg tggtgatgct ggtattgctg
     9001 ctaccgcata ttcacaaagt gaatctttta accctgccgt tgcaacaggg gctggcactg
     9061 tctgcaatcc cggtgatttt tacgtcgttt ggttttcacg gtagcgtgcc gagtattgtc
     9121 agctatatgg atggcaacat tcgtaagcta cgctgggtgt ttataatcgg tagtgccatc
     9181 cccctggtgg catatatttt ctggcaggtg gcgacgcttg gcagcattga ttcaacaacc
     9241 tttatgggat tgctggctaa tcatgctgga ttaaacgggc tgttacaggc gttacgcgaa
     9301 atggtggcct ctccgcatgt tgagctggcg gtgcatttat ttgctgattt agccctcgcc
     9361 acgtcatttc tcggcgttgc gttaggctta tttgattatc tggctgattt gtttcagcgt
     9421 tcaaataccg ttggtggacg attgcaaact ggtgcaatta cctttctgcc gccgttggcg
     9481 tttgcactgt tttatccacg aggatttgtg atggcgctgg gttacgccgg tgtggcgctg
     9541 gcggtactgg cattgattat cccttcacta ttgacctggc aaagcagaaa gcacaatcct
     9601 caggcgggtt accgggtcaa aggtggtcgt ccggcgctgg tggtggtgtt tctctgtggt
     9661 attgctgtga ttggcgtgca atttttgatt gcggcagggt tgttaccaga agtggggtga
     9721 tcagatagcc tcaaattcct tattgggtgc cagaattaac gctgacaccc aatgtgacct
     9781 cttaatgcag gcagcactgc ttaaatttct taccactgcc gcacggacaa ggatcgttac
     9841 gccccggttt ctcttctgct ttgatcggtt gctgaacagc tttttcctgc ggatgcgcca
     9901 tccagtatgc atgtaaatca agcgccgcca gtcgaatggc atctacgctc tcttcaaacg
     9961 cttctggcga cattttctct acccgctcga agttttcctc agtaccgtgc agcgcaatcg
    10021 cctccagcgc tggttttaaa gaatcgggca acgttgacca gtcagaaagt gccacgcccc
    10081 gcatatagcc aaagcaccac tcctcaacaa tcgtcagctc gctgccatca acttctcgca
    10141 agccgaataa cggctcaaac tgctccggga attcgttcag acgctctgcg gtatcggcca
    10201 tatgttgaaa agccaggttc ataaagcgcg tcatctcttt ctctgacgcc caacgcggca
    10261 catagtcagc cccaccccac acggcaacca gccactgttc tggttcaatc tcttgcggag
    10321 aactcaacac cgccgtcaat aaaccgtcca gctccgccac atcaaggatg gcgtggtcag
    10381 tgttgtattt ggtcagaata tcgtccagcc attccaactc actttcgttt aacggtcccg
    10441 ttttcatacg cttttccttg tggatctcaa ctcgccagca cctatcttac atgccggtcc
    10501 gtatcagaga tactttttga gtggctttgc cggtgattaa aaattaagga gggtgtaacg
    10561 acaagttgca ggcacaaaaa aaccacccga aggtggtttc acgacactgc ttattacttt
    10621 gattttattc ttatctttcc catggtaccc ggagcgggac ttgaacccgc acagcgcgaa
    10681 cgccgaggga ttttaaatcc cttgtgtcta ccgattccac catccgggct cgggaagaaa
    10741 gtggaggcgc gttccggagt cgaaccggac tagacggatt tgcaatccgc tacataaccg
    10801 ctttgttaac gcgccaaatt cttcaggcct ttcagccaga catccgcttg acgccgatgt
    10861 cttttaaact ggagcgggaa acgagactcg aactcgagac ctcgaccttg gcaaggtcgt
    10921 gctctaccaa ctgagctatt cccgcattca tcaagcaatc agttaatcac ttgattttat
    10981 tatcgtctgg caatcagtgc cgccgttcga tgcgttgcat tctacttacc tggcgcgatg
    11041 agtcaacgat atttttcacc acttttgatc gtttgctgaa aattacgccg aaacgatcac
    11101 tgatcaagca aatctgcacg cgcagcgctc aaatattgca acattgacca cagagtcagt
    11161 accgcagcca caaagaaaag tgcaataccg acgtactcaa cccaaatgtt cggacgccac
    11221 agcagccagg ccaacgccac catctgggca gtggttttca ctttcccaat ccaggagacg
    11281 gccacgctac tgcgtttacc caactccgcc atccattcgc gtagcgcaga aataataatt
    11341 tcacgggcga tcatcgttgc cgccggtaat gtcacccacc agctgtgata atgctcggtt
    11401 accagcacca tggcgatagc cacgagaact ttatctgcca cagggtcaag gaaagcacca
    11461 aaccgggtac tctggttcca gcggcgtgcc agaaaaccat cgaaccagtc agtcaccgcc
    11521 gcgacgcaga aaatgagcgc ggcggcaaac ggcgaccagg tgacaggcag ataaaagacc
    11581 aatacaaaga atgggataag gatgacacgg aacagtgtaa gcaacgtagg gatattaaat
    11641 tgcataatga cgggtaacta tctgttgtca gtaagattac ccctatgttg ctacagagac
    11701 atcaatgttt caacgaccag aagatctttt ctgccagacc ttgcgaaata cccggcactt
    11761 ttgcaatttc ctcgacgctg gcgttacgta aaccttgcaa accgcccata tatttcaaca
    11821 acatttgccg acgttttggc ccgacgcctt caatggtttc cagggaactg gtatttttga
    11881 ccttcgcccg ttttttacgg tgcccgccaa tcgcgtgatc atgtgattca tcgcgaatat
    11941 gctggataac atgcagcgcg ggtgaatctg gcggcaaact aaatccctca ccttccggct
    12001 caaagaacag cgtttccagt ccagccttac gatctgctcc tttggcaacg ccaagtagca
    12061 gcggatgatt tttatcccat gagacatcca gttcggcgaa gacatttttc gcctgcgcaa
    12121 gctggccttt gccgccgtcg ataaggatca catccgggat cttactgtcg tcaatggctt
    12181 taccataacg ccgacgcagc acctgattca tcgccgcata atcatcgccc ggcgtgatgc
    12241 ctgtaatgtt atagcgccga tactccgcac gcagcgggcc gttagcatca aacaccacac
    12301 aggaagcgac ggtttgttcg cccatggtat ggctgatgtc aaagcactcc atccgcttca
    12361 cttccggcaa tttcaacacg ctggcaagcg cggtcagtcg ctggtgaacg gtagattgct
    12421 gcgaaagttt gctggttaag gccgtcgccg cattggtgcg cgcgagtttc agataacgcg
    12481 ccctatcgcc gcgaggtttg gtttgaacat taatcttgcg tcccgccagt tctgaaaggg
    12541 aatcggcgag cagcgtttta tcgctaagat taaaatcgag caggatctca cccggtaagg
    12601 tgcgcatctg gctgccttgt aaatagaact gacctacgaa ggtttctacc acctcgctca
    12661 gttccgtacc gccaggcact ttcgggaaat agctgcggct gccgagcact ttgccctgac
    12721 gaatgaacaa tacgtggaca caagccatgc ccgcatcgaa cgccacacca ataacgtcaa
    12781 ggtcgtcgcc ggtattggaa acaaactgtt tttcggtgac gcgtcgcacc gcctgaattt
    12841 ggtcgcgaat acatgccgct tcttcaaact ccagattctg gctggcagtt tccatacggc
    12901 taatgagttg cgtaagcacc tgatcatctt tgccagacaa aaacaggcgc acatactcga
    12961 cctgctgagc gtattcttct tcactcacca gtccttcaac gcacggtccc agacagcgcc
    13021 ctatctggta ttgcagacac ggacgcgagc gattgcgata aacactattt tcgcactggc
    13081 gaatggggaa aatcttttgc agtagcgcca gtgtttcacg tacggcatag ccattcggga
    13141 acgggccgaa atattcacct ttggcatgct ttgcaccacg atgcatcgcc agacgcgggt
    13201 gggtatcgcc actcaggaag ataaaaggat aagatttatc atcgcgtagc aaaacgttgt
    13261 aacgcggctg atagagtttg atgtagttgt gttccagcaa cagcgcttcg gtttctgtat
    13321 gcgtaaccgt tacatcaatt tgctggatct gggcgaccag cgcttcggtt ttgcgcgaag
    13381 cgaggttgct gcggaaatag ctggaaagcc gttttttcag gtctttcgct ttgccgacat
    13441 agataaccgt accaccagca tcgtacatgc gataaacgcc tggctggctg gttacggttt
    13501 ttaaaaacgc ttttgcgtca aactgatcac tcactgactt gataatgtct ccgcattaca
    13561 cagaccatgg cgaattgcca ggtgagtcag ctcaacatcg ccatgaatgt ttagtttact
    13621 gaacatacga tagcggtagc tgttcaccgt tttcggactg agattgagct gttctgagat
    13681 ctcattgacc ttctggccct tagtgatcat cagcataatc tgcaattcac gttcagacaa
    13741 actggcaaat gggctttctg ttttttctgg ttcgatctgg cttaacgcca tttgttgagc
    13801 gatatcagaa gcaatgtaac gctgccctga atagacagaa cgaatcgcac tcacgacttc
    13861 ctgcggagcc gcgcctttgc tgaggtagcc agcagcacct gcctgcatga ctttcgctgg
    13921 taaagggttt tctgtatgga ccgtaagcat gatgatttta acatcagctg tggaacgcgc
    13981 gattttacgc gtcgcctcaa gaccgccaat gcccggcata ctcatgtcca ttagcaccac
    14041 gtcaacggca tttgtccggc accacttaac ggcgtcttca ccgcacgatg cctctccgac
    14101 gacttttata cccttaatat cttccagaat gcgtcgtatc cctgcgcgca ccagttcgtg
    14161 gtcatcaaca agtagaacgt tgatcaaagg aatatctcca gaaataggga taacgctact
    14221 gatagttagt cattcgtata ttagcggttt ttattgcaac tttgaaacgt taaaaatggt
    14281 gggtattcga tttttctctc gtttttgtca attcgattgt ccacagttat ggtcacgccc
    14341 gtcaccaacg gcttaccgga tcgtttcaca gtaagcccta cgatgaaatg tgacaaaaat
    14401 tgactttatt cagcaaaaat gaaaatcagc ccgctgaata tgtaacatta attaacccga
    14461 acaatacggg caaaaaacat tcatgataaa aatatttatt ggtcattata ttaacgtatt
    14521 ttatagcact gccgatatca cgctcaaaaa acaaccactg ctatttttag caaagcttat
    14581 ggtatactcc gccgccttaa catttttcac cacaaatttt cattgcaaca tgacgaggaa
    14641 aataaatgag tacgcctgat ttttctactg ccgagaataa tcaagaactg gcaaatgaag
    14701 tctcctgcct gaaagcgatg ctgacgctga tgctgcaggc gatgggacaa gctgacgcgg
    14761 gccgcgtgat gttaaagatg gaaaaacagc ttgcgctgat cgaagacgaa acccaggctg
    14821 cagtattttc caaaacggtt aagcaaatta aacaagccta ccgtcagtaa tataaaaccg
    14881 gctgagagcg tgcctttcag ccggttattg acaaaatcat cgctatctct gctgatgtct
    14941 tcccttccgc cgtccatttc agaatttctt tttcgcgctt gctgaaattc atctctggcg
    15001 tcatcactat ttcatcattt aaacgcatca gagccatcag actttcgcgc accagtaact
    15061 gcatttttaa ttgcagttca tcactaagaa tgggtatttc gcgcgtgctg caacgggaaa
    15121 aggacaaaaa gcccagcgcc cgatttggca gcattaaata ctgagtgaca ccgcggcgta
    15181 aaccatgtgc gtgcgcggct tcccataatg gctgtgcttc gctgaataag tcatcattcc
    15241 acattaaatg gccctgacta aagttttcag ggttcagcac aggatcaatt gcgagaaaat
    15301 tttttgcctg ataataacta acccacgact cagggtaatt ggtgtagaaa gccactttag
    15361 gtcgcgtgaa tggtaccggg tggcgaacac ataacgaata gtaatcgtac tccagctgct
    15421 gagcctgaag ctctatttca tggtaggcct cttctgcggc ctccatcctc tgaaaacgca
    15481 acagcatcgt gcgacgccag ctgaaaaaat ccgtatcctg catagtaaac cgcaacgccc
    15541 ctgagagtga gtatcattta taatgataat tcaaactatc acataaataa gatttatata
    15601 taatttatat tattcaggca atgaattact tttgcaagcc atcgcattct cttatgttat
    15661 taatgagtta tgctgatttg ttaagcagtt ttatcacgct tgaaatggcg tcccgccccg
    15721 acaggtgaat cgtcggggct gattttttct tattattgca gcagaagctt ctcgaggaac
    15781 tggcgggtgc gaggctgctg gggatcggca aataacgctt ttgctgcccc ctgctcgact
    15841 atccgcccct ggtccataaa gatcgcccgg tccgcaacat cccgggcaaa gctcatttcg
    15901 tgtgtcacaa tcaccatcgt gcgcttttcc tgcgccagct ggcggatggt gttcaggact
    15961 tcgcccacca gctctggatc cagcgccgac gttggctcgt caaacaaaat cacctcagga
    16021 cgcattgcca gtgcacgcgc aatcgcaaca cgctgctgtt gaccgccaga caaacgacgt
    16081 ggatagctgg tttctttacc ggccagccca actttcgcca gcagctcgcg agcgcgcgcc
    16141 gtggcctctt ctttcggttc gcctttgacg atcaccggcc cttcaataat gttttccagc
    16201 accgtacgat gcggaaacaa attaaagttc tggaagacaa acccgacgtg ctgacgcaac
    16261 tggcgaatca gcgatttttg ctgacttaat gaacgtgcag tatcaatagt gatatcgcca
    16321 acggtgatcg ttcccgcttc cggttgttcc agcagattta tgctacgtag caatgtggtt
    16381 ttgccggaac cactcggacc gataattgcc accacttcgc caggctttac ctcgaggtcg
    16441 ataccgtgca gcaccgtctg accgtggaat tttttcacca ggttcttaac ttcaatggca
    16501 ctcattttgg ttctctctcc tggcgattaa gttgattctc aaaatggttc tgcaacgtcg
    16561 ataacacagt cgccatgatc cagtagatga gcgaagccgc caggtacatg gtgaacacct
    16621 ccagcgtgcg cgaggtaatc aactgcgcct gacggaacag ctccggcacc tggattgtcg
    16681 cggccagcga cgtatctttt accagactga taaagctgtt cgacagcggc ggcaacgcca
    16741 cccgcgccgc ctgtggcaaa atcgcgcgac gcatggtctg ccacggcgtc ataccaatac
    16801 tggcagcggc ttcccactga cctttatcaa tagaagaaat ggcggcgcgc agcgtttcgg
    16861 cggcataggc ggcagtgttc agcgacagac caatcattgc tgacggaatg ggatctaatt
    16921 cgataccaaa ctgcggcagg ccgtaataga tcataaacag ttgtgcgatg agcggcgtac
    16981 cacgaaaaat agagatataa aaacgcgcca gccagcgcac gggccagatt ggcgacaggc
    17041 gcatcagcgc gagaataaac ccgagtagta agccaaaaaa catgccgccg atactgagtt
    17101 gcagggtata cccagcccct ttcaacagga acggcaagga atcaataacc agttgtatac
    17161 tttcttgcat gagcgttccc ggagctaaac gtggggatga taggcgaaca gcgcaggcgc
    17221 gccgccggta tgaataaaca gaatcggccc ttcatctttg aagcgtttct gactgatacc
    17281 gtcaatcagc cccgccatcg cttttccggt atacacagga tcaagcagaa tgccttccag
    17341 acgcgccagc aatttcactg cttccatgcc ttcgtcgttc ggcacgccgt agccaggtgc
    17401 aaaatagtca tcccagagta aaatttccgc tgatgcggtc agctccagtt ctttcgcaat
    17461 cgcctgttgt aggttaacca ctttcggcaa ttgatcggca acggaacgcg acacggtcac
    17521 gccaatcagt tcgctttcag gcatcaggtg ttccagccca acagccagtc cggcgtgagt
    17581 tccggcactg cccgatgcga ctaccaccga cgaaatatta accgcccctt cacactgttg
    17641 cgcgatttcc agcgcactct ccacataacc tagcgcgcca agagcattag aaccgccaac
    17701 cggaatgaca tacgggcgaa agccttgtgc ttcgactcgc gtcgccagtt cttccagttg
    17761 ggcattggga tcggtcagtg cgtcgcacat ttcaatctgg gtattgaaca gatccagcaa
    17821 caaacgattg ccgttggtta aatagttttc tgcggttgtg ccaataggat tttccagcag
    17881 cgccacgcag tgcagaccga gtttcgccgc gactgcggca gtctggcgca catggttaga
    17941 ctggatcgcg ccggcagtaa tcagcgtatc ggcaccttcg cgcagggcat ctgccgcgag
    18001 aaattccagc ttacgtaatt tattgccgcc cattgccatg ggggtgacct catcccgttt
    18061 gatgaaaatt tcccgtccaa gataatcaga aaagcgcggc agatattcga gcggcgttgg
    18121 cgcgccgata aactccaacc gtggaaaacg ggttaaatta tgcagtggca taacagcctc
    18181 cgatgtgtgt tgttgtgatt ttcttattat gcacgctgaa aacgcgtaaa taaaaaaggc
    18241 gctagtgaaa gcgccctttt ttgtcattat gctgattatt tggtcacatc agcaccaaac
    18301 catttttcgg aaagagcttg cagagtgccg tctttttgca tttccgcaat tgcatcattc
    18361 accgctttca gcaggtcttc atttccttta cgcagcgcca cgccagactc ctgacgggag
    18421 aatgcttcac cggttactgc cagcgtatcg ttggttttct tcaccagatc cagcgccgcc
    18481 agacgatcaa cgaggatcgc atcgatacgc cctacgcgca gatcctgata tttggtcggg
    18541 tcatcatcat aggtacgcac atcgacaccc tgaacattct gccgcagcca ctcttcatag
    18601 ttggtgccca gaccgacacc cacttttttg cctttcagat cagcagccgt tttaatggtg
    18661 ccttcgttac cttttttcac cagcgcctga ataccggaaa tggtgtaagg ggtagagaaa
    18721 tcgtattttt tcttgcgctc gtcagaaatg gtgacctgat taatcaccac atcaatgcgt
    18781 ttagagtcca gcgacgccag cataccgtcc catttggtcg gttttagtga cgcctcaacg
    18841 ccaagatgtt ttgccagctg ttgggcaaat tccacttcaa aaccggttaa tttgccgtca
    18901 tccccctgaa aactgaacgg cggataagtg ccttccagcc ctaccagcag cgtgccgctc
    18961 tctttgactt tattaagcag accttcatct gcaaaacttt taacgctcat gcccgcaacc
    19021 agcgccacgg ccatcacacc catcaatgcc tgacgtccca gatgtgctaa tttcatattc
    19081 accccgaatg ttgttatgtc tgtttgcagt gtagagccat tgtttgtaaa cacaaaaaca
    19141 actccgctac atcttattct tatttaatat atatcagaag caggcaggct ggaggatttc
    19201 tgcacaagtc cggtacaggt ttggcgctga tagtgttgat acttccgtaa tgcgatgcgg
    19261 taattgttgg tgctggtggt gggcagccac ggggcgagga tttcatcaag gaaaccgtcc
    19321 tgcagctgat cgagggaaat attttgctca tgtagatgat tacccaaacg gcgcagacga
    19381 acgacatatt cacgcaccgt acctgggctc atttcagttt gctcaaaaag aaatttctta
    19441 aagccgataa tatcgaaaaa atcactctgc gttttgcaat gtaaatcacc gcaaaatcgg
    19501 cacaatgccg cccatgattg ttgttccgtt tgccagcctt tttcatcaag cagcgtgtcc
    19561 aggcgggaaa tctcgatttt attcactatt ttgccgtcgc gaactaaggt aatgcgatcg
    19621 agtaatttac ggcaatgcgc gcaatgggtc tggctgtgtt taaagtcttt aagatagcgg
    19681 cttaatggcc gtcttttcag gtgctgcacc atcattaaga actcctggta gtcaaagtta
    19741 aagtgcggca tttactgacg ttataactta cccagtttag tgcgtaaccg tttaatggcc
    19801 tggctgtgta actgactgac ccgcgattcc ccgacctcca gcaccgcgcc aatctctttg
    19861 agattcagct cttcctggta atagagggtt aataccagtt tttcgcgctc cggcaacgtt
    19921 tcgatggctt ccatcacccg ctggcgcaga ttactgtcca gcagttgttg tagcgggttt
    19981 tctcgctgat gatcatcagt aaccagttcg atgctatcgc cgtgctcttc acgccactca
    20041 tcgtaggaga agagctggct gttattggtg tcgagcagca tttgacgata atcggcaata
    20101 tcgatcccta aacgttccgc tacctcagtt tccgtggcgt tgcggccaag ttcctgctcc
    20161 agttgcccta ttgcctgtgc cacttcacgc gcgttgcgtc gcacgctgcg cggcacccag
    20221 tcacggctgc gaagttcatc cagcatagcg ccacggatac gctacactgc gtaagttgta
    20281 aatgccgttc cttgtagggc gtcatagcgt tcgacagcat taagtaaccc aatgccgccc
    20341 gcctgtagca gatcgtcaag ttccacgctc gcgggcagtc gaacctgcag gcgcaatgct
    20401 tcgtgacgca ccagcgggac ataacgctgc cacagcgagt gtttatccat tacaccttca
    20461 gcggtataga gtgaattcac gataaacagc cctgcgttat atgagttatc ggcatgatta
    20521 tccgtttctg cagggttttt aatcggacga ttagtgggtg aaatgagggg ttatttgggg
    20581 gttacgcaaa aagccgaatg taaaaaaccc cgccggtggc ggggtttgag aggcattcag
    20641 acgattaacc ctgcagcaga gacagaacct gctgcggtac ctggttggct tttgccagca
    20701 cggagttacc ggcctgctgg ataatctgcg ctttcaacat gttggacact tcggtcgcat
    20761 agtcggcgtc ctgaatacgg gactgcgctt cagacaggtt ggtagtggtg ttgttcaggt
    20821 tggtgactgc ggaatccaaa cggttttgca ccgcacccag ggaggagcgg aatttgtcga
    20881 tggagctgat ggcttcatcc agagctttca ggggatcggc tgtcgtagct gcttttgttt
    20941 cagcatctgt cgtcaattta ccatcagcat ctttataaac tgccttaccg ctgccgttag
    21001 tcactgaacc atcatcctgt aggtaaaaat ctacagtatc agatgtggtc aacgcctcgt
    21061 tgttatcaac ataaaccgaa tcaacacctt ttgttacagc gccagttgtg ccgttgacgc
    21121 tataacctgc agtagtgaaa tcaacattgt ttcccgaggt tgatacagct ttatcgaaag
    21181 ctgcctgatc aacttttgca cctgttactg aataagtaat agtgtccggc gtagtactat
    21241 taccggtaaa ggagatagag gtaccgccaa tgtcaatggt tgtcgctttg gtattattgg
    21301 ctgcaagtgt ggacagacta gcctcagtca aagaagcacc agagccgttt ttcgttaagc
    21361 gcccagttgt atcaatgtag agtttatctc cattgcttgc cgtaatttta ccatcactgc
    21421 tgatctgaac atcctgtgca gaaccatcga tagtaatagt gcccttagcg gtatcaccaa
    21481 ctcccggggt caaatatttc tgaacatcgg cagctgaagc cgttgtggta tcaaaagagt
    21541 atgacttact tgcactgtca tacttgtaat tcgttgcact agccgccgtt ccgaagccgt
    21601 tattaatggt tgcctgaaca gtatcaccat cactaactcc agccagcaaa tcagacgctt
    21661 tagcagcatc gtaacccgca ctcacagtat atttgttgcc gaccactgtt gcattagcag
    21721 ctaccaagtc agctttagat gcagcagtat tagccacagc cccgccacca ttcacattaa
    21781 acccattcag ccccagcgta tcagagtcaa ttttcttcag atcaatagtg atagtctggc
    21841 cgtcattcgc accaacctga attttcatcg aaccgtcttt tgccagtacg ttcacgccgt
    21901 tgaactgggt ctgaccggat acgcggtcaa tttcgtcgag acgggatttg atttcgtcct
    21961 gaatggagtc cagatccgaa tcagagttag tcccggtaga agcctgaacc gtcagctcac
    22021 ggatacgctg taagttgttg ttgatttcgg acagcgcgcc ttcagtggtc tgtgcaacag
    22081 aaataccgtc gttggcgtta cgtgcagcct gagtcaggcc tttaatgtta gaagtaaaac
    22141 ggttagcaat cgcctgaccc gcggcgtcat ccttcgcgct gttaatacgc aagccagaag
    22201 acagacgctc gatagaactc gacagcgcag actggttctt gttgatatta ttttgagtga
    22261 tcagcgagag gctgttggta ttaatgactt gtgccatgat tcgttatcct atattgcaag
    22321 tcgttgatta cgttttgggt ttccacccgt cggctcaatc gccgtcaacc ctgttatcgt
    22381 ctgtcgtaaa acaaccttta gaattttttt cacaaacagc cattttttgt tagtcgacga
    22441 aatactcttt tctctgcccc ttattcccgc tattaaaaaa aacaattaaa cgtaaacttt
    22501 gcgcaattca ggccgataac cccggtattc gttttacgtg tcgaaagata aaaggaaatc
    22561 gcatggcaag tatttcatcg ctgggagtcg ggtcaggtct ggatttaagt tcaatccttg
    22621 atagcctcac cgccgcgcaa aaagcgacgc taacccctat ttcaaatcag caatcgtcgt
    22681 ttaccgctaa acttagcgcc tacggtacgc tgaaaagcgc gctgacgact ttccagaccg
    22741 ccaatactgc attgtctaaa gccgatcttt tttccgccac cagcaccacc agcagcacca
    22801 ccgcgttcag tgccaccacc gcgggtaacg ccatcgccgg gaaatacacc atcagcgtca
    22861 cccatctggc gcaggcgcaa accctgacca cgcgcaccac cagagatgat acgaaaacgg
    22921 cgatcgccac cagcgacagc aaactcacca ttcaacaagg cgacgacaaa gatccgatta
    22981 ccattgatat cagcgcggct aactcatcgt taagcgggat ccgtgatgcc atcaacaacg
    23041 caaaagcggg cgtaagtgcg agcatcatta acgtgggtaa cggtgaatat cgtctgtcag
    23101 tcacatcaaa tgacaccggc cttgataatg cgatgacact ctcggtcagc ggtgatgatg
    23161 cgctacaaag ttttatgggc tatgacgcca gtgccagcag caacggtatg gaggtctcgg
    23221 tcgccgccca gaatgcgcag ctgacggtca acaacgtcgc catcgagaac agcagcaaca
    23281 ctatcagcga cgcgctggaa aacatcactc tgaacctgaa cgatgtcacc acgggcaacc
    23341 agacgctaac catcactcag gacacctcca aagtgcaaac ggcgattaaa gactgggtga
    23401 atgcctataa ctcgctaata gataccttca gcagcctgac caaatacacc gccgtagatg
    23461 cgggagctga tagccagagt tctagcaatg gcgcactgct cggcgactcc acgctgcgga
    23521 cgattcagac gcagttgaag tcgatgctga gtaataccgt cagttcttcc agctataaaa
    23581 cgctggcgca gattggtatc acgaccgatc ccagcgatgg caaactggaa ctggatgccg
    23641 acaaactcac cgctgcactg aaaaaagatg ccagcggcgt aggtgcattg attgttggcg
    23701 atggtaaaaa aaccggcatc acaaccacca tcggcagcaa cctgaccagt tggctttcga
    23761 caacgggcat tattaaagcc gctaccgatg gcgttagtaa aaccctgaat aagttaacta
    23821 aagactacaa cgccgccagc gatcgcattg atgcgcaggt cgcgcgctac aaagaacaat
    23881 ttacccaact ggacgtttta atgacctcgt taaacagcac cagcagctac ttaacgcagc
    23941 agttcgaaaa caacagtaat tccaagtaat cattattcat cgggagacag gtcatgtacg
    24001 cggcaaaagg cacccaggcc tatgcacaaa ttggcgtcga aagcaccgta atgagcgcca
    24061 gccaacagca gttggtcacc atgctatttg atggagtgct gagcgcactg gttagagcgc
    24121 gactgtttat gcaggacaac aatcagcaag gcaaaggcgt ctctttgtca aaagcgatca
    24181 acatcattga gaacggactg cgggtgagtc ttgatgaaga gagcaaagac gaattaaccc
    24241 aaaacttaat tgctctttat agctatatgg tcaggcgttt gctgcaagcc aatttacgca
    24301 acgatgtctc agcagtcgaa gaggtggaag cattaatgcg taatattgcc gatgcctgga
    24361 aagagtcttt actctcccct tcttcgattc aggacccagt ctgatgaaca atgcaccgca
    24421 tttgtatttc gcctggcaac aactcgtcga aaaaagccag ctcatgttac gcctggcaac
    24481 ggaagaacaa tgggacgaac tcatcgctag cgaaatggcg tatgtgaatg cggtgcagga
    24541 gatcgcacac ctgactgaag aggttgcccc gtccactacg atgcaggagc agctccgccc
    24601 gatgctgcac ctgattctcg acaacgaaag caaggtaaag cagttattac agattcggat
    24661 ggatgaactg gcgaaactgg tcggtcagtc atcggtgcaa aaatcggtgt taagtgccta
    24721 tggcgatcag ggcggctttg tgctggctcc gcaggataac ctcttttgaa tcttaatgag
    24781 ccgatggctc gcgaataatc cgattacggc tacgcttcta atattcccct tgaatggagt
    24841 cgaagaatgc gtaatcccac gctgttacaa tgttttcact ggtattaccc ggaaggcggt
    24901 aagctctggc ctgaactggc cgagcgcgca gacggtttta atgatattgg tatcaatatg
    24961 gtctggttgc cgcccgccta taaaggcgca tcgggcggct attcggtcgg ctacgactcc
    25021 tatgatttat ttgatctagg tgagtttgat cagaaaggca gcatccctac taaatatggt
    25081 gataaagcac aactgctggc cgccattgac gccctgaaac gtaatgacat tgcggtgctg
    25141 ctggacgtgg tagtcaacca caaaatgggc gcggatgaaa aagaagctat tcgcgtgcag
    25201 cgtgtaaatg ctgatgaccg tacgcaaatt gacgaagaaa tcattgagtg tgaaggctgg
    25261 acgcgttaca ccttccccgc tcgtgccggg caatactcgc agtttatctg ggatttcaaa
    25321 tgttttagcg gtatcgacca tatcgaacac cctgatgaag atggcatttt taaaattgtt
    25381 aacgactata ccggagaagg ctggaacgat caggttgatg atgaactcgg taatttcgat
    25441 tatctgatgg gtgagaatat cgattttcgc aatcatgccg tgacggaaga gattaaatac
    25501 tgggcgcgct gggtgatgga acaaacgcaa tgcgacggct ttcgtcttga tgcggtcaaa
    25561 catattccgg catggtttta taaagagtgg atcgaacacg tacaggaagt tgcgccaaag
    25621 ccgctgttta ttgtggctga atgctggtcg catgaagtcg ataaactgca aacgtatatt
    25681 gatcaggtgg aaggcaaaac catgctgttt gatgcgccgc tgcagatgaa attccatgaa
    25741 gcatcgcgca tgggtcgcga ctacgacatg acgcagatct tcactgggac gctggtggaa
    25801 gccgatcctt ttcacgccgt aaccctcgtt gccaaccacg acacccaacc gttgcaagcc
    25861 ctcgaagcac cggtcgaacc gtggtttaaa ccgctggcgt atgccttaat tttgttgcgg
    25921 gaaaatggcg ttccttcggt gttctatccg gacctctacg gtgcacatta cgaagatgtc
    25981 ggtggtgacg ggcaaaccta tccgatagat atgccaataa tcgaacagct tgatgagtta
    26041 attctcgccc gtcagcgttt cgcccacggt gtacagacat tatttttcga ccatccgaac
    26101 tgcattgcct ttagccgcag tggcaccgac gaatatcccg gctgcgtggt ggtcatgtcg
    26161 aacggagatg atggcgaaaa aaccattcat ctgggagaga attacggcaa taaaacctgg
    26221 cgtgattttc tggggaaccg gcaagagagc gtagtgacag acgaaaacgg cgaagcgacc
    26281 ttcttttgca acggcggcag cgtcagcgtg tgggttatcg aagaggtgat ttaaatccat
    26341 ccccggcggc aagccgggga gatttcatta cggcagtggc gtcggtaacg gcgctttatc
    26401 cagcgcggcg gcacactctg ccgtcgggcg atccacacgc tccatcgtca taccatcgta
    26461 ctcaatggtg ttaccgtcac gctcaacctc gtacagctca cgttttacgg taacgttagt
    26521 gagatcatca gacatcaatg tcaacttacc cggcacggca atcccccgct gccactgacg
    26581 gcaatccaag gtatcgccct ctttagtgac aatcaggctg cctatcgctt ccggactgac
    26641 cagcgcacgt tgcggccctt tggtctgcca gtagcccgcc agccagtccg gcgcgggggt
    26701 tttcacgaca ttgttatagt tttcgacttc ggcacagccc gccagcaaca tgagcgcgcc
    26761 tgcaattgcg agttttttca tcattcgtcc tgcatgagaa gaaaagagga ttgtggcatt
    26821 aaagctctga tgtcgccagc ctttccaggt tcagagagaa atttgatcta actattattt
    26881 ttgtgaatta ttaccccgaa aataaatttg catggtgtat gattcgcggc tcttgtttca
    26941 aaccttctga gttcagaggc tacattcatg tcatggcagc aattcaaaca cgcctggttg
    27001 attaaattct gggcacccat ccctgcggtc atcgcggcgg gtattctctc cacttactat
    27061 tttggcatta ctggcacctt ttgggctgtc acgggtgaat ttacccgttg gggcggtcag
    27121 ctcctgcagc tgtttggcgt ccatgctgaa gagtggggtt attttaaaat tatccatctg
    27181 gaaggatcgc cattaacccg gatcgacggc atgatgatcc tcggtatgtt tggcggctgc
    27241 tttgctgctg cgctgtgggc caacaatgtc aaactacgca tgccacgcag ccgtatccgc
    27301 attatgcagg ccatcattgg cggcattatc gccggttttg gcgcgcgtct ggcaatgggc
    27361 tgtaacctgg cggcgttctt taccggcatt cctcagttct cgctgcatgc ctggttcttt
    27421 gccatcgcca ctgccattgg ttcatggttt ggcgcgcgct ttaccctgct gcccatcttc
    27481 cgtattcccg tgaaaatgca gaaagtttct gccgcctcac cgctgacgca aaaaccggat
    27541 caggcgcggc gtcgttttcg tctcgggatg ctggtctttt tcggcatgct gggctgggcg
    27601 ctgctcacgg cgatgaacca accaaaactg gggctggcaa tgctgtttgg cgtcggcttt
    27661 ggtttactga ttgaacgtgc gcaaatctgc tttacttcgg cgttccgcga tatgtggatc
    27721 accggacgta cccatatggc gaaagcaatc attatcggta tggcggtgag tgccatcggg
    27781 atcttcagtt acgtacagtt aggcgttgaa cctaaaatca tgtgggcggg accaaatgcg
    27841 gtaattggtg gtttactttt tggttttggc atcgtgctgg caggcggctg cgaaaccggc
    27901 tggatgtacc gcgcggtaga aggccaggtg cactactggt gggtcggtct gggcaacgtg
    27961 atcggctcaa cgattctggc gtattactgg gatgatttcg ctccggcgct ggccaccgac
    28021 tgggacaaaa tcaacctgct gaaaactttt ggtccgatgg gaggcctgct ggtgacatat
    28081 ttgctgctgt ttgctgcgct gatgttgatt atcggctggg aaaaacgctt cttccgccgt
    28141 gaggcaccgc agactgctaa ggagatcgca tgaaaaatat cgttcctgat taccgccttg
    28201 atatggtggg tgaaccctgc ccttatccgg cagtcgcaac ccttgaggcg atgccgcaat
    28261 taaaaaaagg tgagatcctg gaagtggtga gcgactgtcc gcagtcgatc aataatattc
    28321 cactggatgc gcgtaatcac ggctacacgg tgctggatat tcagcaagac gggccgacca
    28381 ttcgttattt aattcagaag taatccctca ttcccgctgt ggcccacagc gggaacatcg
    28441 ttacctctga cacatagatt ttacccgccc cctctcctat tcttttcagt atgatctcag
    28501 gaggttgcta tgtgtggacg ctttgcccaa tcccaaacgc gtgaggatta ccttgcgctt
    28561 ctcgcggaag atattgaacg cgatattccc tacgatcccg aacccattgg cagatacaat
    28621 gtcgcgccgg gaaccaaagt tcttctgctc agtgaacgtg atgaacacct tcatctggat
    28681 ccggttttct ggggatatgc tcccggatgg tgggataaac cgccgctgat taacgcccgc
    28741 gtagaaactg cctcaaccag tcgcatgttt aaaccgctct ggcaacatgg tcgggcaatc
    28801 tgttttgccg atggctggtt tgagtggaaa aaagaaggcg acaaaaaaag ccttatttta
    28861 tctatcgcgc tgacggacaa cctgttttta tggccgcgat aggtagcata ccgtttgagc
    28921 gtggtgacga agccgaagga tttttgatcg tcactgcggc ggcagatcaa ggtctggtag
    28981 atattcatga ccgccgcccg ctggttctgt cgccagaagc tgcacgggaa tggatgcggc
    29041 aagacattgg tggtaaagaa gcctcagaaa tcgctaccag aagctgtgtg ccagcaaacc
    29101 agttcacctg gcaccctgtg tcgcgcacgg tgggtaatgt caaaaaccag ggcgcggagt
    29161 taattcaacc tgtttgttga catcctccac gccctgaatg acgaggaccc ctgctacgtt
    29221 caggctgtcg cctgaatcat ttcggtgggt tcctgcttca acgagcggcc tgactgcacc
    29281 atctctccac aggcaagaac ggcgtgcccc gccgctaaaa tgttacgagc tccatttaca
    29341 tcggcgttcg ctgtatatcc acatacctgg catctgaatt tactttgtga caggcgattt
    29401 tctttcgctg tatgaccaca gcacgcgcaa cgctggcttg tgtacgctgg cggaacagca
    29461 agcacctgac cgccacgcca gagctgcttg tactcaagct ggcggcgcat ttcataccag
    29521 ccctgatcca gtatcgaacg gtttaaacct gattttgccc ggacattgcg ccccggctgg
    29581 ctgaccgtac ccgctgctga ctttgacatg ttgctgacct tcaaatcctc aatgactatc
    29641 attacgtggt ttttgctgac ggtcgttgtg actttgtgaa ggtagtccct gcggatattt
    29701 gcgatacagg aatgcagtcg ctgtattttg cgtttctgct tctgccagtt gttgctgaat
    29761 ttgaccttgc ggcttaactg tcgctgaagt ctcgccagcg tcttctggtt tttctgaaaa
    29821 ctgtttacag gctcaaagac tgtgccatct gacagcgagg caagtttagc cacgccagca
    29881 tccagcccga ccattgatgc tgaagggtga accggagttg atacttcact ttctgcctga
    29941 atactgatgt accacttacc gcaggactgg ctgacagtga catttttcac aacacccgtg
    30001 acctgacggc tgttccggta gcgcatccag ccaagttttg gcagaaaaat acggctgttt
    30061 tcctgagcga gcttaacgcc ctgcggatag cggaatgcat cattctgtca ccgctttttg
    30121 aatcagggga acgccgagcg cttctggaaa aagtttttgc aggcctgctc agggtctttc
    30181 agtgactgtt gcaatggctg tgacggagca tctttaatcc attgcgtttc agtggcattt
    30241 ttccactcaa ccaatcagga agccattttc gcgtaaggga tgtctttgtt accggcctta
    30301 tgattctcat tctgacgtgc cagagtacga ctgaaaacga aacgacatgc gcctgcgaag
    30361 cgcctcattt cacgcgcctg ttgaccaccg ggtcttaacc ggaatttgaa tacctgtagt
    30421 cgtttcacag ggcaaattat actttggtct gtgaaaaaaa ctgatatccg acgcggaagg
    30481 cactgtgttt tcctgatgca tgctcaccgg gtctttgtta ccaagtaccg acgccagatt
    30541 tttgaccatg acgcgacaga aaaactacgc acttactttt caaatgtatg tgcttatttt
    30601 gaagctgaac tggttgaaat ggatggcgaa ccagatcacg tccatctgtt aattaactat
    30661 ccccccaaac tggcgatatc cagtctggta aacaaacagc ctcaaaggcg tatcgggtag
    30721 gttactgcga cgggatcgac cagatattgc tgccaggtat taccgcaaag atgttttgtg
    30781 gcgtcccggc tattttgcca gtagctgcgg aggtgcgcca atatccgtca tccgccaata
    30841 cattgaacaa cagcaaacac ctggtcagat gaaaaaacga gccttatatc cccgccctga
    30901 aggacgggct tttacggtgc actggataag cctggagatt atctacacct gcatggacat
    30961 cattgtacta tgccccgtac atgctccatc acatcctcaa catcaaagta ttagagcctt
    31021 gcaaaccatt atgcgtctca gtttgtctca tctatcttac gcaaaaaaaa tagaactaat
    31081 atcaagcggc gctatattgc gtattaaata aaaccacctc aattacatta ataaatctaa
    31141 ataaatatca tgcaaataat ttccatggtt atttttaggc tatttaaatt aaaaaaataa
    31201 attatgagtc aatcataaat aacaaccttt aatataattt gactaccaaa tttatattcg
    31261 ttcacaaaat agtcatgcat tgtgtgcaat agaaacagtt cagataaaaa tagggatcta
    31321 cttcacaatt taaatgagaa actaaaactt acatcttgaa ataatctcat tgattggatg
    31381 aatatttgta gtgcagtgac atcattttta aataatagtt caaa
//
LOCUS       DAJTSA010000039        31086 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000039 DAJTSA010000000
VERSION     DAJTSA010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.039"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            389..2047
                     /locus_tag="OO369_001964"
     CDS             389..2047
                     /locus_tag="OO369_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR5982249.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2044..3000)
                     /locus_tag="OO369_001965"
     CDS             complement(2044..3000)
                     /locus_tag="OO369_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR5982250.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3151..4266
                     /gene="macA"
                     /locus_tag="OO369_001966"
     CDS             3151..4266
                     /gene="macA"
                     /locus_tag="OO369_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR5982251.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4263..6209
                     /gene="macB"
                     /locus_tag="OO369_001967"
     CDS             4263..6209
                     /gene="macB"
                     /locus_tag="OO369_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR5982252.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="OO369_001968"
     CDS             complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="OO369_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR5982253.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6829..7149
                     /gene="clpS"
                     /locus_tag="OO369_001969"
     CDS             6829..7149
                     /gene="clpS"
                     /locus_tag="OO369_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR5982254.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7180..9456
                     /gene="clpA"
                     /locus_tag="OO369_001970"
     CDS             7180..9456
                     /gene="clpA"
                     /locus_tag="OO369_001970"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR5982255.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9799..9886)
                     /locus_tag="OO369_001971"
     tRNA            complement(9799..9886)
                     /locus_tag="OO369_001971"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9850..9852),aa:Ser,seq:gga)
     gene            complement(10140..10358)
                     /gene="infA"
                     /locus_tag="OO369_001972"
     CDS             complement(10140..10358)
                     /gene="infA"
                     /locus_tag="OO369_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR5982256.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10643..11347)
                     /gene="aat"
                     /locus_tag="OO369_001973"
     CDS             complement(10643..11347)
                     /gene="aat"
                     /locus_tag="OO369_001973"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR5982257.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11389..13110)
                     /gene="cydC"
                     /locus_tag="OO369_001974"
     CDS             complement(11389..13110)
                     /gene="cydC"
                     /locus_tag="OO369_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR5982258.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13111..14877)
                     /gene="cydD"
                     /locus_tag="OO369_001975"
     CDS             complement(13111..14877)
                     /gene="cydD"
                     /locus_tag="OO369_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR5982259.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15000..15965)
                     /gene="trxB"
                     /locus_tag="OO369_001976"
     CDS             complement(15000..15965)
                     /gene="trxB"
                     /locus_tag="OO369_001976"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR5982260.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16509..17003
                     /gene="lrp"
                     /locus_tag="OO369_001977"
     CDS             16509..17003
                     /gene="lrp"
                     /locus_tag="OO369_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR5982261.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17138..21127
                     /gene="ftsK"
                     /locus_tag="OO369_001978"
     CDS             17138..21127
                     /gene="ftsK"
                     /locus_tag="OO369_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR5982262.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEP
                     LYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPV
                     KEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSG
                     GPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQ
                     YNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQ
                     QRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQ
                     QPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPV
                     DTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLA
                     RSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDI
                     AGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELS
                     VYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRM
                     MRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKAR
                     AAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDM
                     LYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGA
                     EELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNR
                     EVLAPPPFD"
     gene            21282..21893
                     /gene="lolA"
                     /locus_tag="OO369_001979"
     CDS             21282..21893
                     /gene="lolA"
                     /locus_tag="OO369_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR5982263.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21904..23247
                     /gene="rarA"
                     /locus_tag="OO369_001980"
                     /gene_synonym="mgsA"
     CDS             21904..23247
                     /gene="rarA"
                     /locus_tag="OO369_001980"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR5982264.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23338..24630
                     /gene="serS"
                     /locus_tag="OO369_001981"
     CDS             23338..24630
                     /gene="serS"
                     /locus_tag="OO369_001981"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR5982265.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24870..27313
                     /gene="dmsA"
                     /locus_tag="OO369_001982"
                     /pseudo
     CDS             24870..27313
                     /gene="dmsA"
                     /locus_tag="OO369_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27324..27941
                     /gene="dmsB"
                     /locus_tag="OO369_001983"
     CDS             27324..27941
                     /gene="dmsB"
                     /locus_tag="OO369_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5982266.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27943..28734
                     /locus_tag="OO369_001984"
     CDS             27943..28734
                     /locus_tag="OO369_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5982267.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28770..29396)
                     /locus_tag="OO369_001985"
     CDS             complement(28770..29396)
                     /locus_tag="OO369_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5982268.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29711..30859
                     /locus_tag="OO369_001986"
     CDS             29711..30859
                     /locus_tag="OO369_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5982269.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7663 a         7616 c         8347 g         7460 t
ORIGIN      
        1 tggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
       61 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
      121 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
      181 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
      241 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
      301 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa
      361 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
      421 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
      481 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
      541 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
      601 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
      661 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
      721 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
      781 cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt
      841 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
      901 cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
      961 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
     1021 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
     1081 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
     1141 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
     1201 tactttattg caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct
     1261 aattgaagat ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt
     1321 gaatcttttg cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac
     1381 tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct
     1441 ggggccaagt ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa
     1501 tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
     1561 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
     1621 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
     1681 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
     1741 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
     1801 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
     1861 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
     1921 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
     1981 cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
     2041 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
     2101 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
     2161 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc
     2221 gttgtaatca gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
     2281 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
     2341 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
     2401 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
     2461 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
     2521 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
     2581 gaactgctca ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
     2641 taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc
     2701 ctccagcaat tgcttacggc taagattcgc ggcaaggtaa gggcgatgca gacgcacagg
     2761 cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
     2821 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
     2881 acagcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
     2941 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
     3001 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
     3061 agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc
     3121 ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
     3181 tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac
     3241 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
     3301 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt
     3361 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
     3421 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
     3481 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
     3541 cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg
     3601 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
     3661 caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg
     3721 gctggggaag tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa
     3781 gcaccgaaca ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct
     3841 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat
     3901 ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac
     3961 gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg
     4021 gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct
     4081 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
     4141 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
     4201 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
     4261 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
     4321 aggttgaggt gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg
     4381 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
     4441 ccaccagcgg cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc
     4501 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgcttccgc
     4561 atttaaccgt cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac
     4621 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
     4681 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
     4741 tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg
     4801 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
     4861 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
     4921 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
     4981 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
     5041 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
     5101 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
     5161 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
     5221 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
     5281 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
     5341 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
     5401 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
     5461 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
     5521 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
     5581 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
     5641 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
     5701 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
     5761 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
     5821 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
     5881 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
     5941 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
     6001 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
     6061 ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct
     6121 gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg
     6181 atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc
     6241 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
     6301 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
     6361 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
     6421 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg
     6481 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
     6541 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
     6601 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
     6661 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
     6721 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
     6781 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
     6841 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
     6901 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
     6961 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
     7021 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
     7081 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
     7141 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
     7201 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
     7261 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
     7321 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
     7381 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
     7441 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
     7501 gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
     7561 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
     7621 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
     7681 cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca
     7741 ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
     7801 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt
     7861 gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct
     7921 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
     7981 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
     8041 cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc
     8101 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
     8161 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
     8221 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
     8281 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
     8341 tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga agcaggcgct
     8401 cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
     8461 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
     8521 accctgaaaa aactcagcga tcgcctgaaa atgctggtct tcggccagga taaagccatt
     8581 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
     8641 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
     8701 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
     8761 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
     8821 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
     8881 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
     8941 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc
     9001 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
     9061 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
     9121 atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc
     9181 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
     9241 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
     9301 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
     9361 ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag
     9421 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga
     9481 agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc
     9541 ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa
     9601 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
     9661 tatttgagtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
     9721 ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
     9781 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
     9841 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta
     9901 ccaaacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga
     9961 agaattttaa tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac
    10021 atcgagacaa gttatggact tatacttcca aagtacttca tacatatcac aaaataaaaa
    10081 ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat
    10141 cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc
    10201 actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca
    10261 gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga
    10321 acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta
    10381 ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc
    10441 taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa
    10501 aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt
    10561 ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg
    10621 gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga
    10681 aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg
    10741 gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct
    10801 taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt
    10861 ccatccggct gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac
    10921 cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa
    10981 ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg
    11041 cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt
    11101 tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg
    11161 gatcgggcga ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa ataccacgct
    11221 ggtaagccat taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag
    11281 gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca
    11341 agcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg
    11401 cttgaactgg taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat
    11461 aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg
    11521 ggtgaccatt aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct
    11581 ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg
    11641 taacagcgta cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc
    11701 ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg
    11761 caagatctcc gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg
    11821 cagtgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc
    11881 cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc
    11941 ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat
    12001 cgctatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg
    12061 ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt
    12121 ttgggtatca gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc
    12181 agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa
    12241 cgcggctaac gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc
    12301 aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat
    12361 tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat
    12421 ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc
    12481 ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt
    12541 ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa
    12601 cataacgctg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac
    12661 caccataatc accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc
    12721 gagcgtatca acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag
    12781 tccggcaggg gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac
    12841 gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat
    12901 tgcggcacca cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac
    12961 ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc
    13021 gagcagcgtc acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata
    13081 cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa
    13141 tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg
    13201 gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac
    13261 cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact
    13321 gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa
    13381 cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc
    13441 aacaggtgta tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc
    13501 cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg
    13561 caattttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga
    13621 ttctggtgat aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag
    13681 aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac
    13741 tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg
    13801 cgacgtgata aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc
    13861 ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc
    13921 accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa
    13981 ctctggcgca aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg
    14041 accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga
    14101 cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat
    14161 tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg
    14221 accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg
    14281 agcaagagcg agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat
    14341 aaacaacgga attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg
    14401 gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata
    14461 gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc
    14521 cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg
    14581 gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac
    14641 cacccatgcg cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc
    14701 acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc
    14761 aatgatcaat atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga
    14821 gatgacgctt tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc
    14881 acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat
    14941 aaaggcgacc catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat
    15001 tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca
    15061 gtaccggccg aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag
    15121 acgccaggaa tgctggtctg ggtggcatta ccatgaatac ccgactgtac tttgatgtag
    15181 ccgttttcca gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca
    15241 acaaacagac cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga
    15301 cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc
    15361 agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg
    15421 cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc
    15481 gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag
    15541 aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc
    15601 aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg
    15661 ccgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca
    15721 aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga
    15781 ccggtcagat cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg
    15841 ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca
    15901 gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg
    15961 cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac
    16021 gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca
    16081 ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat
    16141 taaatgatga ataaacgccc ctgttaatga atatctggca tgttgtacta aaaatcgatg
    16201 ttttgctttg acaatcccct ggtgttttgc gaaaacattc gaggaagaaa aaaacagtat
    16261 tcttatatgc gcataaccat gtatgtaaat accatgttta ccgtgctagt gaaatctacg
    16321 tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc
    16381 accgtagacc caggcattgc gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt
    16441 agcgactctg aacagtgatg tttcagggtc agacaggagt agggaaggaa tacagagaga
    16501 caataataat ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta
    16561 acattcttaa tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg
    16621 tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga caagggttta
    16681 ttcagggcta tacggcgctg cttaaccccc attatctgga tgcatcactt ctggtattcg
    16741 ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat accgctgtac
    16801 aaaaacttga agaaattcag gagtgtcatt tagtatctgg tgatttcgac tacctgttga
    16861 aaacacgcgt gccggatatg tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc
    16921 tgcctggcgt caatgacaca cggacatacg ttgttatgga agaagtcaag cagagtaatc
    16981 gtctggttat taagacgcgc taacacggaa caggtgcaaa atcggcgtat tttgattaca
    17041 ctcctgttaa tccatacagc aacagtactg gggtaacctg gtactgttgt ccgttttagc
    17101 atcgggcagg aaaagcctgt aacctggaga gcctttcttg agccaggaat acactgaaga
    17161 caaagaagtc acattgacaa agttaagtag cggccgccgc cttctggagg cattgctgat
    17221 ccttattgtc ctgtttgccg tctggttgat ggctgcctta ctaagcttta acccttcgga
    17281 ccccagctgg tcgcaaacgg cctggcatga acctatccat aatttaggtg ggatgcccgg
    17341 tgcgtggttg gcagatacgc tgttctttat ttttggcgtg atggcttaca ccattcccgt
    17401 cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag tccagcgacg aatacattga
    17461 ttattttgcc gtttcgctac gcatcattgg cgttttggcg ctcatcctta cctcctgtgg
    17521 tctggcggca atcaacgctg acgatatctg gtattttgcc tccggtggcg tcattggcag
    17581 cttactaagc actacgctac aaccactgct acacagtagc gggggaacta ttgcgctgct
    17641 ctgcgtttgg gcagcgggcc tgacgttgtt caccggttgg tcatgggtga ccattgctga
    17701 aaaactcggc ggctggattt taaacattct caccttcgcc agtaatcgta cccgtcgcga
    17761 tgatacctgg gtcgatgaag atgagtatga agacgacgaa gagtatgaag atgaaaatca
    17821 cggcaaacag catgaatcac gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa
    17881 acggttggcg gaaaaattca ttaatccgat ggggcggcaa acagacgctg cgttgttctc
    17941 cggtaagcgg atggatgatg acgaagagat tacctacact gcacgcggtg tggctgccga
    18001 cccggacgac gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg acgaatacga
    18061 tccattatta aacagtgcgc caattaccga acctgtcgct gtagcagctg ctgctaccac
    18121 ggcgacacaa agctgggctg cgccggttga acctgtgact cagacgccgc ctgttgcctc
    18181 tgttgatgtt ccaccttcgc aacctacagt agcctggcag cctgtaccgg gtccacaaac
    18241 gggagagccg gttattgctc ctgcaccgga aggttaccca cagcagtcac aatatgcgca
    18301 gcctgcagtg caatataatg agccgctgca acaaccagta cagccgcagc agccgtatta
    18361 tgcccctgcg ccagaacaac cggtggcagg taacgcctgg caagccgaag agcagcaatc
    18421 cacttttgct ccacagtcta cataccagac tgagcaaact tatcagcagc cagccgctca
    18481 ggagccgttg taccaacagc cgcaacccgt tgaacagcag cctgttgtgg agcctgaacc
    18541 cgttgtagaa gagacaaaac ccgcgcgtcc gccgctttac tattttgaag aagtggaaga
    18601 gaagcgagcc cgtgaacgtg aacaacttgc ggcctggtat caaccgattc cagaaccggt
    18661 taaagaacca gaaccgatca aatcttcgct gaaagcacct tctgttgcag cagtacctcc
    18721 agtagaagcc gctgccgctg tttctccgct ggcatctggc gtgaaaaaag cgacactggc
    18781 gacgggggct gccgcaaccg ttgccgcgcc agtcttcagt ctggcaaata gcggtggacc
    18841 gcgtccgcag gtcaaagagg ggattggccc gcagttgcca cgaccgaaac gtatccgcgt
    18901 gccaactcgt cgtgaattgg cgtcttacgg tattaagctg ccctcacagc gtgcggcgga
    18961 agaaaaagcc cgtgaagccc agcgcaatca gtatgattct ggcgatcagt ataacgatga
    19021 tgaaatcgat gcgatgcagc aggatgaact ggcacgtcag ttcgcccaga cacagcagca
    19081 acgctatggc gaacagtatc aacatgatgt gcccgtaaac gcagaagatg cagatgctgc
    19141 ggcagaggct gaactggctc gtcagtttgc gcaaactcaa caacaacgtt attccggcga
    19201 acaaccggct ggggcgaatc cgttctcgct ggatgatttt gaattttcgc caatgaaagc
    19261 gttgctggat gatggtccac acgaaccgct gtttacgcca attgttgaac ctgtacagca
    19321 gccgcaacaa ccggttgcac cacagcagca gtatcagcag ccacaacagc cggttgcgcc
    19381 acagcagcag tatcagcagc cacaacagca ggttgcgccg cagccacaat atcagcagcc
    19441 gcaacaaccg gttgcgccac agcagcaata tcagcagccg caacaaccag ttgcgccgca
    19501 gccgcagtat caacagccgc aacaaccggt tgcaccgcag ccgcagtatc agcagccaca
    19561 acagccagtt gcgccacagc cgcaggatac cctgcttcat ccgctgttga tgcgtaatgg
    19621 cgacagccgt ccgttgcaca aaccgacgac gccactgcct tctctggatt tgctgacacc
    19681 gccgccgagc gaagtggagc cggtagatac ctttgcgctt gaacaaatgg cgcgtctggt
    19741 ggaagcgcgt ctggctgatt tccgtattaa agccgatgtc gttaattact ctccggggcc
    19801 agttatcact cgctttgaac ttaacctggc tcctggcgta aaagcggcgc gcatttctaa
    19861 cttgtcacgg gaccttgccc gttcactttc gacggtggcg gtgcgtgtcg ttgaagttat
    19921 tcctggcaaa ccctatgtag gtctggagtt accaaataaa aaacgacaaa ccgtttatct
    19981 gcgcgaagtt ttggataacg ccaaattccg cgataatccg tcgccattaa ccgtggtgct
    20041 gggtaaagat atcgccggtg agccggtggt tgccgatctg gcgaaaatgc cgcacttgtt
    20101 ggttgcgggg actaccggtt ccggtaaatc tgtcggtgtg aacgcgatga tcctgagcat
    20161 gctttataaa gcacagccag aagatgtgcg tttcatcatg atcgacccga aaatgctgga
    20221 gctttcggtt tatgaaggca ttccgcacct gttaacggaa gtcgttactg atatgaaaga
    20281 tgccgccaac gcgctgcgct ggtgtgttaa cgagatggag cgtcggtata aactgatgtc
    20341 tgcgctgggt gtgcgtaatc tggcgggtta taacgaaaaa attgctgaag ccgatcgcat
    20401 gatgcgtccg attccagacc cgtactggaa gccgggtgac agtatggatg cccagcatcc
    20461 ggtgctgaaa aaagaaccat acattgtcgt gttggttgac gaatttgccg acctgatgat
    20521 gacggtaggt aaaaaagtgg aagagctgat agctcgtctg gcgcaaaaag cccgtgccgc
    20581 gggtatccac ctcgtactgg caactcagcg tccatcggtt gatgttatta ctggtctgat
    20641 taaagcgaat attccgaccc gtatcgcctt taccgtatcc agtaagattg actcacgtac
    20701 cattcttgat caggctggcg cggaatcact gctgggtatg ggggatatgc tctactctgg
    20761 gccgaactcc acgttgccgg tacgtgtcca tggtgctttt gttcgcgatc aggaagttca
    20821 tgccgtggtg caggactgga aagcgcgtgg tcgcccacag tatgttgatg gcatcacctc
    20881 cgacagcgaa agcgaaggtg gtgcgggtgg tttcgatggc gctgaagaac tggatccgtt
    20941 gttcgatcag gcggtgcagt ttgtcactga aaaacgcaaa gcgtcaattt ctggcgtaca
    21001 gcgtcagttc cgcattggtt ataaccgtgc agcgcgtatt atcgaacaga tggaagcgca
    21061 ggggattgtc agcgaacagg ggcacaacgg taatcgtgaa gtgctggccc caccgccgtt
    21121 tgactaatta atgcattgcc ggataaggcg cggtagcgtc gcatccggca ctctatcaac
    21181 tgaaaattca gtattttctt ctttcctcaa gctgattatt agcctggaat agagagtaga
    21241 gggaactccc gatcgggagt gacgtaattt gaggaataat gatgaaaaaa attgctatca
    21301 cctgtgcatt actctcaagc ttagtagcaa gcagcgtttg ggctgatgcc gcaagcgatc
    21361 tgaaaagccg cctggataaa gtcagcagct tccacgccag cttcacacaa aaagtgactg
    21421 acggtagcgg cgcggcggtg caggaaggtc agggcgatct gtgggtgaaa cgtccaaact
    21481 tattcaactg gcatatgaca caacctgatg aaagcattct ggtttctgac ggtaaaacac
    21541 tgtggttcta taacccgttc gttgagcaag ctacggcaac ctggctgaaa gatgccaccg
    21601 gtaatacgcc gtttatgctg attgcccgca accagtccag cgactggcag cagtacaata
    21661 tcaaacagaa tggcgatgac tttgtcctga cgccgaaagc cagcaatggc aatctgaagc
    21721 agttcaccat taacgtggga cgtgatggca caatccatca gtttagcgcg gtggagcagg
    21781 acgatcagcg cagcagttat cagctgaaat cccagcaaaa tggggctgtg gatgcagcga
    21841 aatttacctt caccccgccg caaggcgtca cggtagatga tcaacgtaag tagaggcacc
    21901 tgagtgagca atctgtcgct cgatttttcg gataatactt ttcaacctct ggccgcgcgt
    21961 atgcggccag aaaatttagc acagtatatc ggtcagcaac atttgctggc tgcggggaag
    22021 ccgttgccgc gcgctatcga agccgggcat ttgcattcta tgatcctctg ggggccaccg
    22081 ggtaccggca aaacaaccct cgctgaagtg attgcccgct atgcgaacgc tgatgtggaa
    22141 cgtatttctg ccgtcacctc tggcgtgaaa gagattcgcg aggcgatcga gcgcgcccgg
    22201 caaaaccgca atgcaggtcg ccgcactatt ctttttgttg acgaagttca ccgtttcaac
    22261 aaaagccagc aggatgcatt tctgccacat attgaagacg gcaccatcac ttttattggc
    22321 gcaaccactg aaaacccgtc gtttgagctt aattcggcac tgctttcccg tgcccgtgtt
    22381 tatctgttga aatccctgag tacagaggat attgagcaag tactaactca ggcgatggaa
    22441 gacaaaaccc gtggctatgg tggtcaggat attgttctgc cagatgatac acgacgtgcc
    22501 attgcagaac tggtgaatgg cgacgcgcgc cgggcgttaa atacgctgga aatgatggcg
    22561 gatatggccg aagtctatga tagcggtaag cgggtcctga agcctgaatt actgaccgaa
    22621 atcgccggtg aacgtagcgc ccgctttgat aacaaaggcg atcgctttta cgatctaatt
    22681 tccgcactgc ataagtcggt acgtggtagc gcacccgatg cggcgctgta ctggtatgcg
    22741 cgaattatta ccgctggtgg cgatccgtta tatgtcgcgc gtcgctgtct ggcgattgcg
    22801 tctgaagacg tcggtaatgc cgatccacgg gcgatgcagg tggcaattgc ggcctgggat
    22861 tgctttactc gcgttggccc ggcggaaggt gaacgcgcca ttgctcaggc gattgtttac
    22921 ctggcctgcg cgccaaaaag caacgctgtc tacactgcgt ttaaagccgc gctggccgat
    22981 gctcgcgaac gcccggatta tgacgtgccg gttcatttgc gtaatgcgcc gacgaaatta
    23041 atgaaggaaa tgggctacgg gcaggaatat cgttatgctc atgatgaagc aaacgcttat
    23101 gctgccggtg aggtttactt cccgccggaa atagcacaaa cacgctatta tttcccgaca
    23161 aacaggggcc ttgaaggcaa gattggtgaa aagctcgcct ggctggctga acaggatcaa
    23221 aatagcccca taaaacgcta ccgttaatgt tatcgttgcg gtaatgttgt tactgtatcc
    23281 ctgtggtcgc aggctgtggc cacatctccc atttaattcg ataagcacag gataagcatg
    23341 ctcgatccca atctgctgcg taatgagcca gacgcagtcg ctgaaaaact ggcacgccgg
    23401 ggctttaagc tggatgtaga taagctgggc gctcttgaag agcgtcgtaa agtattgcag
    23461 gtcaaaacgg aaaacctgca agcggagcgt aactcccgat cgaaatccat tggccaggcg
    23521 aaagcgcgcg gggaagatat cgagccttta cgtctggaag tgaacaaact gggcgaagag
    23581 ctggatgcag caaaagccga gctggatgct ttacaggctg aaattcgcga tatcgcgctg
    23641 accatcccta acctgcctgc agatgaagtg ccggtaggta aagacgaaaa tgacaacgtt
    23701 gaagtcagcc gctggggtac cccgcgtgag tttgactttg aagttcgtga ccacgtgacg
    23761 ctgggtgaaa tgcactctgg cctcgacttt gcagctgcag ttaagctgac tggttcccgc
    23821 tttgtggtaa tgaaagggca gattgctcgc atgcaccgcg cactgtcgca gtttatgctg
    23881 gatctgcata ccgaacagca tggctacagt gagaactatg ttccgtacct ggttaaccag
    23941 gacacgctgt acggtacggg tcagctgccg aaatttgctg gcgatctgtt ccatactcgt
    24001 ccgctggaag aagaagcaga caccagtaac tatgcgctga tcccaacggc agaagttccg
    24061 ctgactaacc tggtgcgcgg tgaaatcatc gatgaagatg atctgccaat taagatgacc
    24121 gcccacaccc catgcttccg ttctgaagcc ggttcatatg gtcgtgacac ccgtggtctg
    24181 atccgtatgc accagttcga caaagttgaa atggtgcaga tcgtgcgccc agaagactca
    24241 atggcggcgc tggaagagat gactggtcat gcagaaaaag tcctgcagtt gctgggcctg
    24301 ccgtaccgta aaatcatcct ttgcactggc gacatgggct ttggcgcttg caaaacttac
    24361 gacctggaag tatggatccc ggcacagaac acctaccgtg agatctcttc ctgctccaac
    24421 gtttgggatt tccaggcacg tcgtatgcag gcacgttgcc gcagcaagtc ggacaagaaa
    24481 acccgtctgg ttcataccct gaacggttct ggtctggctg ttggtcgtac gctggttgca
    24541 gtaatggaaa actatcagca ggctgatggt cgtattgaag taccagaagt tctgcgtccg
    24601 tatatgaacg gactggaata tattggctaa tacccaattt ttctgaatct aaaaagcgcc
    24661 tttagggcgc tttttttgtc tccctttgat accgaacaat aattactcct cacttacacg
    24721 taatactact ttcgagtgaa aatctaccta tctctttgat ttttaaatta ttctatgtat
    24781 acaagcctat atagcgagct gctatagaaa taattacaca atacggtttg ttactggaat
    24841 caatcgtgat caagcttgag tgagccatta tgaaaacgaa aatccctgat gcggtattgg
    24901 ctgctgagga gagtcgccgt ggtttggtaa aaacgacagc gatcggcggc ctggcaatgg
    24961 ccagcagcgc attaacatta ccttttagtc ggattgcgca cgctgtcgat agcgccattc
    25021 caacaaaatc agacgaaaag gttatctgga gcgcctgtac agttaactgt ggtagtcgct
    25081 gcccgctacg tatgcacgtc gtggacggtg aaatcaaata tgtcgaaacg acaataccgg
    25141 cgatgacaat tacgacggcc tacactaggt tcgcgcctgc ctgcgtgggc gttccatgcg
    25201 tcaccgtgtc tacaatccgg accgcctgaa atatccgatg aaacgagtcg gggcgcgcgg
    25261 tgaaggcaaa ttcgagcgca ttagctggga agaagcctac gacatcatcg cgaccaatat
    25321 gcagcgcctg atcaaagagt acggcaacga gtctatctat ctgaactatg gcaccggtac
    25381 gctgggcggc accatgaccc gctcctggcc gccgggaaat accctggtcg cgcgcctgat
    25441 gaactgctgc ggcggttatc ttaaccatta cggtgactac tcttccgcgc aaattgcgga
    25501 aggtttgaac catacctacg gcggctgggc agatggtaac agcccgtcgg atatcgaaaa
    25561 cagtaaattg gtagtgctgt ttggtaataa cccaggcgaa acgcgaatga gtggcggtgg
    25621 ggtgacttac tatcttgaac aggcacgcca gaaatctaat gctcgcatga tcatcatcga
    25681 tccgcgctat accgacaccg gtgccgggcg cgaagatgag tggatcccta ttcgtccagg
    25741 aacagatgcc gcactggtta acggtctggc gtacgtcatg atcactaaaa acctggtgga
    25801 tcaggcattc ctcgataaat attgcgttgg ctatgacgag aaaaccctgc ccgccagtgc
    25861 gccgaaaaat ggtcattata aagcttatat tctgggtgaa gggccagatg gcgtggctaa
    25921 aacaccggaa tgggcctcgc aaatcaccgg tgttccggca gacaaaatca tcaaactggc
    25981 tcgtgaaatc ggcagtacca aaccggcgtt tatcagccag ggatggggcc cgcagcgtca
    26041 cgctaacggt gaaatcgcaa cccgtgctat ctcgatgctg gcgattctga ccggtaacgt
    26101 tggtattaat ggagggaaca gcggcgcgcg tgaaggttca tacagcttac cgtttgtccg
    26161 tatgccgacc ttggaaaacc cgatccagac cagcatttcg atgtttatgt ggaccgatgc
    26221 cattgaacgt ggcccggaaa tgacggcgct gcgtgatggt gtgcgcggga aagataagct
    26281 ggatgtgccg atcaaaatga tctggaacta tgccggtaac tgcctgatta accagcattc
    26341 tgaaatcaac cgtacccatg aaatcctcca ggatgataag aagtgcgagc tgattgtggt
    26401 tatcgactgc cacatgacct catcggcgaa atatgctgac atcctgctgc ctgactgcac
    26461 cgcttccgaa cagatggact ttgcactgga tgcatcctgc gggaatatgt cttacgtgat
    26521 tttcaacgat caggtgatta aaccgcgctt tgaatgtaag accatctatg aaatgaccag
    26581 cgaactggca aaacgtcttg gcgttgagca acagtttact gaaggccgta cccaggaaga
    26641 gtggatgcgg catctgtatg cccagtcgcg ggaagcgatt cctgaactgc caacgtttga
    26701 agagttccgc aagcagggga tctttaaaaa gcgcgaccca caagggcatc acgttgctta
    26761 taaagccttc cgtgaagatc cgcaggcaaa cccactgact acgccatcgg gcaaaattga
    26821 gatttattcg caggcgctgg ctgacattgc cgctacctgg gaattgccag aaggcgatgt
    26881 gatcgatcca ctgccgatct acacgccggg ctttgaaagt tatcaggatc cgctgaacaa
    26941 acagtatccg ctgcagctta caggtttcca ctataaatct cgcgttcact caacttacgg
    27001 caacgttgat gtgctgaaag cggcttgccg tcaggaaatg tggatcaacc cgcttgatgc
    27061 ccaaaaacgc ggtatccaca acggcgataa agtcaggatc tttaacgatc gtggtgaggt
    27121 tcatattgag gcgaaagtga cgccacgaat gatgccgggc gtggtcgcac tgggtgaagg
    27181 cgcctggtat gacccggatg caaaacgtgt cgataagggc ggttgtatca acgtactgac
    27241 cactcaacgt ccgtctcctc tcgctaaggg gaatccgtca catacaaacc ttgttcaggt
    27301 tgaaaaggtg taaggagtaa ccgatgacaa cccagtatgg attttttatt gattccagcc
    27361 gttgcaccgg ttgcaaaacc tgcgagctgg cttgtaaaga ctacaaagat ttgacgccag
    27421 aagtcagttt ccgccgcatt tatgaatatg ctggcggcga ctggcaggaa gataacggtg
    27481 tctggcacca gaacgtgttt gcctactatc tgtcgatttc gtgcaaccac tgcgaagatc
    27541 cggcttgtac taaagtctgc ccgagcggtg cgatgcataa acgcgaagat ggttttgttg
    27601 tggtcgatga agatgtatgc attggctgcc gttactgtca tatggcttgc ccgtatggcg
    27661 caccgcaata taacgaaacg aaaggccata tgaccaaatg cgatggttgt tatgaccgtg
    27721 ttgcggaggg taaaaagccg atctgtgttg aatcttgccc gctgcgggcg ctggatttcg
    27781 gccctatcga cgagctgcgt aaaaaacatg gcgatctggc ggccgttgcg ccgttgccgc
    27841 gagctcactt taccaaaccg aatattgtga tcaaacccaa tgccaatagc cgcccgaccg
    27901 gggataccac tggctatctg gcaaacccga aggaggtgtg agatgggaag tggatggcat
    27961 gaatggccgc tgatgatctt cacggtcttc gggcaatgtg tggcgggtgg ttttatcgtt
    28021 ctggctttgg cgctgctcaa aggcgacctg cgagcagaag cccagcagcg tgttatcgcc
    28081 tgcatgtttg gcttatgggt actgatgggc attggcttta tcgcctctat gctccatctt
    28141 ggttcaccga tgcgcgcctt taactcgctc aaccgggtag gggcttcagc actcagtaac
    28201 gaaatcgcca gcggttcgat cttttttgct gtaggcggca tcggctggtt gctggcaatg
    28261 ctgaaaaagc tgtcaccggc attgcgtacg ctatggctgg tggtgacgat ggttcttggc
    28321 gtcgtctttg tctggatgat ggtgcgtgtg tataacagca ttgataccgt tccgacctgg
    28381 tacagcatct ggacgccgat gggctgggcg atgcgtctgc tgccagccat ttctgtactg
    28441 gcactggtgg tgagtggcgt gatgtcagtg atgcagggca cagagctggc aactattcat
    28501 agttctgtgc agcaggccgc agcgctggtg ccggactatg gtgcgctgat gtcctggcgg
    28561 atcgtgcttt tggccgttgc cctgtgcttg tggattgcac cacagctaaa aggttatcag
    28621 cctgcggttc cgctactatc cgtctcattc attctgcttc tggcagggga attaatcggt
    28681 cgcggcgtat tctatggttt gcatatgacc gtggggatgg ccgtcgcaag ctaatcagta
    28741 acaaccgggg tttcggcccc ggttctcttt tatttctgct tcgttaacgt gtcataactg
    28801 gtcatcaaat tacgataatc aggaatatgg ttagagaaca acgtcgccag tccttcaata
    28861 tcattacgcc agtcacggtg cagctcgcaa gccacgccga accatgtcat caattgcgca
    28921 ccagcttgcg acatgcgatc ccacgccgaa tgccgggtaa tttcattaaa ggtccctgat
    28981 gcatcagtca cgacaaagac atcaaaccct tcttcaatgg ctgaaagcgc cgggaatgcc
    29041 acgcaaactt cggttaccac accggcaata attaactgtt ttttacctgt cgctttgact
    29101 gcttttacaa aatcttcgtt atcccaggcg ttaatattgc ccgggcgagc aatgtaaggt
    29161 gcatcgggga attgtgcttt cagttctaga actaatgggc cgttaggacc ggtttcaaaa
    29221 ctggtggtaa gaatagttgg taagttgaaa tacttagcca ggtcacccag cgccagcacg
    29281 ttatttttaa acttatcggg ttcgatatcc cgtacaaggg aaagtaaacc agcctggtga
    29341 tcaacaagca aaacggcagc atcattttta tcaagacgaa catacggttt ggtcatcctc
    29401 ttctcctttc gagagtcagt ggtacgtctg aaatcggtca gacgcgatgc attgctctga
    29461 aagcatagac gggaaatatg agtttgctgt gaccatgaaa tttttcgact gaacgaagcg
    29521 tatgaaattt gtgttagatc aataaaaaca atcagatgag attaatcatc tcttcagcat
    29581 tagcgttatg aatgagtaat ataaatcgat aataccgccg ctgcgatatg cgcgtatttt
    29641 cacccattga caatgttttt ggcggtggca tgatgcgcat gaaatttgaa cttcctcacg
    29701 gttttaattc atgtccacgt atacccggcc tgtcatgctt ttgctgtctg gcctgctttt
    29761 gttgactctg gcgatcgcgg tgttaaatac actcgtgccg ctttggctcg cccaggaaca
    29821 catgtccaca tggcaggtag gcgttgtcag ctcatcctat tttaccggca accttgtcgg
    29881 tacattgctg acagggtatg tcattaaacg cattggcttt aaccgcagct attatctgtc
    29941 ctccttcatt tttgctgctg gctgtgccgg ccttggcctg atgattggtt tctggagctg
    30001 gttggcgtgg cgttttgtcg cgggcgtcgg ctgtgccatg atttgggtgg ttgttgagag
    30061 cgcgctgatg tgcagtggga cgtcacgtaa ccgtgggcgt ttgcttgctg cgtatatgat
    30121 ggtttattac gtgggaacgt ttttaggcca gttactggtc agcaaagtct caaccgagct
    30181 gatgagcgta ttgccgtggg ttacaggttt gacattggca gggatcttac cgctgttgtt
    30241 tacgcgtgtc ctgaatcagc aggctgaaaa ccatgattcg acgtcaatta cggcaatgct
    30301 aaaactccgt caggcgcggc ttggcgtgaa tggctgcatt atctcaggaa tcgttctggg
    30361 atctctatat ggcctgatgc ctctgtacct caatcacaaa ggggtgagca atgccagtat
    30421 tggtttctgg atggcggtac tggtcagtgc gggtatcctc ggacaatggc cgattggacg
    30481 tctggcggat aagtttggtc gactgctggt tttgcgtgtt caggtctttg tcgtcattct
    30541 cggcagtatt gcgatgctta gccaggcggc gatggcccca gcgttattca tcctcggtgc
    30601 cgctggcttt acgctatatc cggtggcgat ggcctgggct tgtgagaaag ttgaacatca
    30661 tcaactggtg gcgatgaacc aagccttact gttgagctat accgtgggaa gtctgcttgg
    30721 cccgtcattt agcgctatgc taatgcagaa tttctccgat aatttattgt ttatcatgat
    30781 cgccagcgta tcgtttatct atttgctgat gctgcttcgc aacgccggtc atacgccgaa
    30841 acccgttgct cacgtgtaaa tgaattcaag caaagtgtga acttactgtt tcacactctg
    30901 cttttttgtt tcttctatct gacttgcttt tttccaaatt ttattcgttt aaaaataaaa
    30961 tgtgcagcag gttataattt tgcatttcgc tatttccgca cttcttattt gccgcgcata
    31021 atccctcgtt ttacagatgc ccctttaatt ttggcgaagg atttgtctat ggctgggaat
    31081 gttcag
//
LOCUS       DAJTSA010000040        30295 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000040 DAJTSA010000000
VERSION     DAJTSA010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.040"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..815)
                     /gene="wcaD"
                     /locus_tag="OO369_001987"
     CDS             complement(<1..815)
                     /gene="wcaD"
                     /locus_tag="OO369_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR5982270.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="OO369_001988"
     CDS             complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="OO369_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR5982271.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="OO369_001989"
     CDS             complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="OO369_001989"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR5982272.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="OO369_001990"
                     /pseudo
     CDS             complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="OO369_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="OO369_001991"
     CDS             complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="OO369_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR5982273.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="OO369_001992"
     CDS             complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="OO369_001992"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR5982274.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6041..7180)
                     /gene="wza"
                     /locus_tag="OO369_001993"
     CDS             complement(6041..7180)
                     /gene="wza"
                     /locus_tag="OO369_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR5982275.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7489..7638
                     /locus_tag="OO369_001994"
     CDS             7489..7638
                     /locus_tag="OO369_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5982276.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7839..9422
                     /locus_tag="OO369_001995"
     CDS             7839..9422
                     /locus_tag="OO369_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5982277.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9874..11727)
                     /gene="asmA"
                     /locus_tag="OO369_001996"
     CDS             complement(9874..11727)
                     /gene="asmA"
                     /locus_tag="OO369_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR5982278.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11749..12330)
                     /gene="dcd"
                     /locus_tag="OO369_001997"
     CDS             complement(11749..12330)
                     /gene="dcd"
                     /locus_tag="OO369_001997"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR5982279.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12422..13063)
                     /gene="udk"
                     /locus_tag="OO369_001998"
     CDS             complement(12422..13063)
                     /gene="udk"
                     /locus_tag="OO369_001998"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR5982280.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13381..13623
                     /locus_tag="OO369_001999"
                     /pseudo
     CDS             13381..13623
                     /locus_tag="OO369_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            13622..14563
                     /gene="mdtA"
                     /locus_tag="OO369_002000"
                     /pseudo
     CDS             13622..14563
                     /gene="mdtA"
                     /locus_tag="OO369_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            14563..17685
                     /gene="mdtB"
                     /locus_tag="OO369_002001"
                     /pseudo
     CDS             14563..17685
                     /gene="mdtB"
                     /locus_tag="OO369_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17686..20751
                     /gene="mdtC"
                     /locus_tag="OO369_002002"
     CDS             17686..20751
                     /gene="mdtC"
                     /locus_tag="OO369_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR5982281.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20752..22167
                     /locus_tag="OO369_002003"
     CDS             20752..22167
                     /locus_tag="OO369_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5982282.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            22164..23567
                     /gene="baeS"
                     /locus_tag="OO369_002004"
     CDS             22164..23567
                     /gene="baeS"
                     /locus_tag="OO369_002004"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR5982283.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            23564..24286
                     /gene="baeR"
                     /locus_tag="OO369_002005"
     CDS             23564..24286
                     /gene="baeR"
                     /locus_tag="OO369_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR5982284.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            24477..24809
                     /locus_tag="OO369_002006"
     CDS             24477..24809
                     /locus_tag="OO369_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR5982285.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24956..26317
                     /gene="yegQ"
                     /locus_tag="OO369_002007"
     CDS             24956..26317
                     /gene="yegQ"
                     /locus_tag="OO369_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR5982286.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26659..26976)
                     /locus_tag="OO369_002008"
     CDS             complement(26659..26976)
                     /locus_tag="OO369_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5982287.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            27382..28281
                     /gene="yegS"
                     /locus_tag="OO369_002009"
     CDS             27382..28281
                     /gene="yegS"
                     /locus_tag="OO369_002009"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR5982288.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(28363..29136)
                     /locus_tag="OO369_002010"
     CDS             complement(28363..29136)
                     /locus_tag="OO369_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5982289.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(29242..30282)
                     /gene="gatD"
                     /locus_tag="OO369_002011"
     CDS             complement(29242..30282)
                     /gene="gatD"
                     /locus_tag="OO369_002011"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5982290.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
BASE COUNT         7009 a         8049 c         7829 g         7408 t
ORIGIN      
        1 cctaaatcgc ccagacgggt ggaaatataa ggaaacgcga ttaccacacc aacccggaat
       61 acagccagag aaatcaatgc taacggtaat tttttcttaa tcgcctcttt attcagatat
      121 tgaaatgccc actccagcaa ataaaacagg ataaaggtca taacccctga aaacgatccg
      181 gataatatta tccctgcgag aatcagggca tctgttttag gcgttttgat accaaactgt
      241 ttgatgctga gccaaattga gattaatgcc agagcgaaaa atgccggttc gaaataaagt
      301 gctgtcgtgc gcttgccgcc gaatttaatg aaattcagca catagctgtt gctgtaaatc
      361 agatatttcg aaatcgactc cataatactg ctgccaccgg tgaggataat ttgcgccatc
      421 tccaccgccg ccagcgccac caccagtccg accaccagat aaaagaaacg taatatctta
      481 cgatggttgt gtggcgaaat tgttttaaaa cgaatactcc acaccatgcc aataatgatc
      541 acaatataga caaacagcat agttgaagtg acgtatttac cggcatccaa cgactgacca
      601 aacaggtagt taaacgccgt gagtcccgcg ccaatcccta aggcaatcat caatttttta
      661 atgctgatgc gttctaaaaa cagcagtaac aagacaggta aaaaagtgac gatggtgatg
      721 gggaaacttt cgccaagctg ggcgattttg acgttaacca gcaggtagat cagtggcagc
      781 agcaggtagc tacagattct gatagaagtt gacatactcc tccagcatct gttgtccact
      841 gtaggcggcg cgactgcgtt ggctgaactc agccagcgtg gtaccaaata tcgcctgcgc
      901 gatttccggt ttgcttaact gtaccagttg cagcacctct tcttcactga cggttttacc
      961 accggatttt tgcagcactt cccgcgccgc atcgctatgg gtggcaatca ccggcacgcc
     1021 aatcgatagc gcctcacaca aaatcagcgg gtagttatcg acacgagaac tgaacaccag
     1081 cgcatccatc tgattgagcg cgctcatcaa cttgcgcttg tcagtttcaa agccgtgatt
     1141 aaccacgttg ccagcggtga acggcgagaa cttaccaaag gtatgcagtt cgattttgtc
     1201 gcccagcgcc atcatctcac gcaccagttg ctggttagtt ttgccgtcgt aacgcaggtc
     1261 atgcgccacc actgcgattt tcggcttgcc ctgagtttcg cgcaccggag gcagatctgc
     1321 cagaatcgct tcggttgcca tatcaatgcc attattgata atccggcaac gccctggccc
     1381 gtacagacta ttgaaagcgt cagccacatg ctggctgggg gaaataaact gacagcccag
     1441 cgccagcatc tcgcggaata actggcgttt gcccgccacc agctggtgcg cgcgatcaat
     1501 cttcaccggc ggatagttat ttaaggtcgg gcatttctgg cagcccgttt tccagccttc
     1561 gcaaccgtcg gtaaaggcgc agcgtccggt tacactccag tggtcgtgca gcgtccagac
     1621 cagggtgaca tccggtttat ggtttttcac tttttcgcaa aagcgcacca cgctgttaag
     1681 attcaaccag tagctgtgca gcacatgaaa atgcaggacc accggacccg gtgtgcgagt
     1741 aatggtgcga tataactcat tgaaattgcc gaacagatcg cgattaaaca gacgaaacaa
     1801 cgcaatgttc gccatcgcgg tcatccgcgg cgtatgtttg atgacctgcg gatagttctg
     1861 atggctgacg ctctctttgc cgcctttgcc gtagccgtag acaaaatgtg acgccagccc
     1921 ctgttgcagc gcacgctggt ggagatctaa cgccacacct gctgccccgc cttccgccag
     1981 tcgcacatta aattgcagaa tattcattta atcaccttca ctcgcgcttt ttctcccacc
     2041 accagcgcgt tgtccgggac agagtcgagc accacgctgc ccgcacccac ggtgacgttg
     2101 ttaccaagcg tgatatcacc aagaataatg acgttggcac cgagttcgac gccgttgcca
     2161 atgtgtggac atgccatgtt atcggcaccc cgattgccga tagtgacgcc gtggcgaatg
     2221 gtgaaatcat cccccgccac cacgttttta ttgatcacta cggcgtaacc gtgatggata
     2281 gtaaagcggc ggccaatggt cgcggcggcc tggatttcat aaccgaaaaa gcattcggtg
     2341 ataatgcgat acagcaccag cagcggggcc gcccacagat tgttgaggac gttctttttg
     2401 cgccacaccg aacaaaaatg agcgacgcga taggcaagta ccatgcagca cgggcgtaaa
     2461 ctccagctgt tggcgcgcag atcttccagc atctttagcg cccccgaata ccatcagcca
     2521 gacgtttgcc gttacgcacc gacagcagcg tcaacaaggt gcgccaggtc atacgtttat
     2581 tgcggatctg gtagagggta aacagctgat attttttact ggcgcggtcg aatttgtctt
     2641 tgtgcttgcg gtaaaagtgg aagtatccgg agaatttttt cggtgacgag gtgatctgca
     2701 tttcgccgtg attgatatgc aggatctgcg tcgcctcttc cactttccac ggttcgccgt
     2761 actccaccac catccgcagg aatatgtcgt aatcctgtgc tgctttcagt tcggtatcga
     2821 acaaacactc tttgaaacgc catgcccagg taaagacctg attgccaatg atattacgtt
     2881 tatagaacag gcggcgtgag tacggcgatt tgggatacag tggtaagctc gccggttggg
     2941 aataaacttc tccctagcaa acgtagtcgt tagcgtacaa aaaggcgtgt gtgaccagtt
     3001 gctgtttatg ggcgaggaag acgctcaggc ggttgggcgt ccattcatca tcatcatcga
     3061 tcccggtgat gtattcccct tgcgccagca taatcgcctg gttacgcacc gcgcacgccc
     3121 cgctgttaat gtcgttgtgg atgtaagtga tacgcggatc gttgagggca gtgacgtact
     3181 gttgcagttg ttcccaggag gtggagcaat catccacgat gatcatctcc cagttgctgt
     3241 agtcctggcg caggaccgat tttatcgccc gaatcgccag ttgttggcgg ttccaggttg
     3301 gcatatagat tgagatcagc ggattgtctt tcataggttg ttctcccggc ttatgagctg
     3361 gtttgcttgc cggatgcggc gtgagcgcct tatccggcct acggggcggt gcgaatgcag
     3421 gcgtcgttat ttcgcatccg acttatattc gtattcgtaa tagccataat cctgatacgc
     3481 gctggcgcgg cggaagatgg agttcagaat cacccctttc accggaatac cgttttgctc
     3541 aaagcggctc agactggttt ccacttcttt caatgtgttg accgcataac gcgccaccat
     3601 taacgtggtt ccgacatgac gaccaacaat tgccgcatcg gtcactgcca gaatcggcgg
     3661 cgtatcaatc aacaccaggt catagttttt actcgcccag ttcaccagtt cggcaaagcg
     3721 ttcgctcatt aacagttcag aaggatttgg cggcacctga ccgcgcggga tcaggtcaaa
     3781 tttggcaata gaggtcggtt tagcagcggt agtaatatcg ccctgaccaa tcagaatctc
     3841 cgacagccca ttgacattat tggtgcccaa cagctcgtgg gtgtagcctt tgcgcatatc
     3901 gcagtcgatc aacaacacgc gtttattggt ctggctgatc accgccgcca ggttggcgca
     3961 gacaaaggtt ttaccgattg acgggctaac cccggtcatc atcaacacat tgttctgcgc
     4021 ctgcatcatc gcgaagtgca gactggtacg cagactacgg atggcttcaa tcgccagatc
     4081 ggttggattc cccactgcca gtagctggct ctgtttatag cgtttaaccc ctttgatggt
     4141 tttgacgcta tcgcgcgctt tctgccactc cgacagcggg atgctggcat agacgctgat
     4201 tccgtgttct tccagcactt gcgggctttc gatgccgcga ttaaacaacg agcgcagcag
     4261 cacacccacg atagagagca tcaggccaag gataatcgcg ccgaggataa tcagcccttt
     4321 cttcggtttt agcacgccag gctgggtgat tgccgggtca ataatgcgca catcgccgac
     4381 ggtgctggct tcggtgattt tcagctcctg ctctttattc agcagttgca tatagacctg
     4441 ctgaccagac tcgacatcgc gggtcagacg gacaatttcc tgctggtttt tcggcatcgc
     4501 cgttacgcga ccgttaagtt tggctttctc ttcttccagc gcctgacgtt tctccagcag
     4561 cgtgcggtac gccgggtgaa ctttggtgta cagcttggag atttccgcct ctttaaaggt
     4621 cagttcgttc aactgcgcgt cgatgttcac catcgaatcg agcaccgctt tcgcttcaag
     4681 cggcagatca acagaatctt tatcctgacg gaaggcattc agtttgtttt cggcaacatc
     4741 gaggcggcta cgtacttccg gtaactgttg cgcgaggaaa gcgaggcttt tcgacgcttc
     4801 cgccgatttg cgctcaatat tttgttcctg atagttacga gcgatgctgt taagaatgtc
     4861 gcggatctgt tcgcgatctt caccggtata agtcaggctc agtacgcctg cgtctttgcc
     4921 gttctccgtt accgtcaggc tgttttgcag ttggttgatc atccccaacg tggagtattt
     4981 ggtgacggta aactcactgc ccgggcgggc gtgaatggct tcaaccatca gcgtgacgcc
     5041 ttcttttttc agcatctggc ccgcttgccc acgggcgcta aagccgccat cactgctcag
     5101 ggtgtagttt ttgtcatcca gcacattaag cgtaaacacc tgatccgcca tctctttcgg
     5161 gcggttaaag gtagtcactt tcaccgtctc gttctgacgc cccatcaggc gatcccagcc
     5221 cgccccgaaa atcgggaacg tgtttttgct caccgcaata tcgaggtcga gatcgtccac
     5281 cgttttacca agcaccagac gcgaacgaat caactggatc tcggcgtccg atgcaggcgg
     5341 tttgttggct aatgccgagc cgatatcctg caccagcgaa ttgccgctgt tttgctcgat
     5401 ttgtaccagt gcgtcggcac tataaatcgg cgtggcgaag aaagtgtaaa ccacggcaca
     5461 gagggcaaat acgacggtga tgccaatcac ccaccagcgc gcttcaatga cggtgccgac
     5521 caggcgacca atatcgattt catcactgcc cgttaccgga gcggcatgtt gttttacttt
     5581 ttctgtcatt cttatacctg ctctgcgttc aatgcctgcg cccactggcg ggcagaccgt
     5641 tcaagtaatg tgtacaccgc tgcaaacgtt tcccggcttt tgtgatacgg atcggggatt
     5701 tcacattcgt tatcccagtg accaaacagc atcactttgc cgcgcatctc cggtgccatc
     5761 tcgcataagc gttcgatatg gcgcttatcc atggtcaaaa tcaggtcata gttgcgacac
     5821 aggcggcggc tgatttgtcg tgcacagtgg ccttccagag acagttgatg ttctgcggcg
     5881 acgctgatag cggtaggatc agcgccctta ccgaccagcg cgccgagtcc agcggactcc
     5941 actttcagtt ccgggtgata acgttgcagt aagcgttccg ccatcgggga acggcaaata
     6001 ttgccgacac agacaactaa gatgttgtta aacatgacga ttaccagtta tgaatgtcgc
     6061 tggctgtatc cgtcatgtaa cggacaccgc taatagttgg cagcaactga ttgatcagac
     6121 ggttccagcg ggaaaccggg gcggtggtga catacaccac gtcataaggt tgcaggcgga
     6181 attctgtcgc catcaccagc gacgtcgcat cggacatatc cagctggtag atattggcaa
     6241 tcttgccgtt acgcccgccc tcgcctttca gcggacgaat gacaaagatg ccgctggcgt
     6301 tggaggtggt catgtcgata ccttcggcat tggccagagc ttcagtcagg gtcatgccgc
     6361 taaagtccat tttcagggtg ctctgtttct tcacttcacc catcacaaat actttcagat
     6421 catcattacg cggcacgtag agaatatcgc cgggataaag cagtcggttc tgattgaggt
     6481 cgccgttttg catcagcgcc tgtaaagaaa tgcgttcttc acgaccattg tgtgttagca
     6541 ccacgttgcg ccagtcagcg gtgtcggtca ggccacctgc ggcgttgatg gcgtcgagaa
     6601 tggtcagtgg tacgttggtg atcgcctgtt gaccggattt attcacctga cctgagatat
     6661 aggccttttg tgagcggaag gcggcgatat taacgtccac ctgcgggtca gcgatgtacg
     6721 tcgctaagcg cccggtaata tcactgcgga tttcagcgag cgttttcccg actacgtgaa
     6781 ccttgccgat atacgggtaa aacatagtgc cgtcaggctg tacccagttg ccggtgtcgc
     6841 tggagctgcg gtactgacca gctggcgtgg tgagttccgg gtgatcccag acggtgacat
     6901 taagaacgtc ccccggcccg acgcgatact ggtaattcgc gatctcgctt tccagcgtca
     6961 tattggggcg cgctacattc gggcgtgggc gtaattggtc aatcaggcgc ggggtcagcg
     7021 gataaacatt caccattttg tcgagatcga aatcagcgtc ctgctgtttg atgacgtctt
     7081 tgcccatcgt cgacatattg ctgcccggaa gtactgtgca accgcttatc aaggttactg
     7141 acaccaataa tggcatcaat ttcattttgg atttcatcat tgtttattta tcactttggc
     7201 agagtaatta tcctgtgcac tattaatagc aatgtcgcca tgcacattta ccttgcagtt
     7261 aattgaataa aaatttaact ggcatcagtc ctaaaaaaat tgatttcatc cgcaggctat
     7321 tgacagaata attcagactg gtctttcagg catccagaca cgctaccgcc cctggctttt
     7381 tagctaccaa tacactgatt gagcttaatt tttcaaaccc tctctgcata caatcgttga
     7441 tgagaaaggg ttattgcgga aattaacttc cgaatataag gtgacattat ggtaattgaa
     7501 tattggctcg ccaataatgc aggaggaagt gttacagcta acggaatagc aggcaaggta
     7561 acaattcggc aattggctat ttttaagaat tatattaagt atcattcaat atggttttta
     7621 ggagtttctt taggttgaca atatttaata ttgcgtctcc acatgcgata tttcttaaat
     7681 aatgttttat tattaccgct tttaattcag ggataatgtg aggttattac cctcaaataa
     7741 tatgagataa atagtgctgc aacattgcat ttttgccccg atttatccat gatcgaattg
     7801 tgacatttgt catacaacga ataggttttg tacttactat ggaatggatt gccgatccgt
     7861 ctatctgggc cgggttaatc acgctgattg tgatcgaact ggtcctcggc attgataacc
     7921 tggtctttat tgccatcctc gccgaaaaac taccgccgaa gcagcgcgac cgcgcacggg
     7981 ttaccgggct gctgctggcg atgttaatgc gcctgttact gctagcgtca atctcctggc
     8041 tggtcaccct gactcaaccg ctgttcagct tccgctcgtt tacctttagc gcccgcgact
     8101 taatcatgct gtttggtggt ttcttcctgt tgttcaaagc cacgatggag ctgaacgaac
     8161 ggctggaagg gaaagacagc aataatccca cacaacgcaa aggtgcgaag ttctggggcg
     8221 tggtgacgca aattgtggta ctggacgcca ttttctcact cgactcagtg attaccgccg
     8281 tcgggatggt cgaccattta ctggtcatga tggccgccgt ggttatcgct atcagcctga
     8341 tgttgatggc cagcaagccg ttaacgcaat ttgttaacag tcacccgacc atcgttattc
     8401 tctgcttaag cttcctgttg atgattggct ttagcctggt ggcagaaggt ttcggcttcg
     8461 tcattccgaa aggctacctg tacgctgcca ttggtttctc ggtgatgatc gaggcactca
     8521 atcagttggc tatctttaac cggcgacgtt ttctttccgc taatcagacg ctacgccagc
     8581 ggactaccga agcggtaatg cgcctgctta gcgggcaaaa agaagatgcg gaactggacg
     8641 ccaaaaccgc gtccatgctg gtggatcatg gaaaccagca gatctttaat ccgcaggaac
     8701 ggcggatgat tgagcgggta cttaatctta accagcgtac cgtaagcagc attatgacgt
     8761 cgcgccacga tattgagcat atcgatctca acgcgccgga agaagaaatc cgccagttgc
     8821 tggagcgaaa tcagcatacg cggctggtgg ttaccgacgg tgatgacgca gaagatttgc
     8881 tcggtgttgt tcacgttatc gacctgctac aacagtcact gcgcggcgaa ccgctcaacc
     8941 tgcgggtgtt gattcgccag ccgctggtgt tcccggaaac cttgccgttg ttaccagccc
     9001 tggagcagtt ccgtaatgcc cgcacgcact ttgcttttgt ggtggatgag tttggctcgg
     9061 tggaagggat tgtgacatta agtgacgtca ctgaaaccat tgccggtaac ttaccgaacg
     9121 aagtggaaga gatcgacgcc cgccatgata ttcagaagaa tgcggacggt tcctggacgg
     9181 cgaatggtca tatgccgctg gaggatctgg tgcaatatgt gccgctgccg ctggatgaaa
     9241 aacgtgaata tcacaccatt gccgggctgt tgatggagta tttgcagcgt attccaaaac
     9301 ctggcgaaga agttcaggtg ggggattatt tgcttaaaac gttgcaggtg gaaagccatc
     9361 gcgtgcagaa ggtgcagatt ataccgctgc gtaaggatgg cgagatggag tacgaggtgt
     9421 gatggtatcg gccattttac ccaatgctgc gtaacgcctt ataacttcag caatgaagcg
     9481 ttgtctgtgg taaacggaca tcacagcggg ggtgtgggtc cggctgtaaa tcgccgaatc
     9541 gtttcctgta ggctggggca gcccacatgg atgtgggctg agggcgcgtt ttacagggat
     9601 gttacctcgc gcccgtaccc gatagccgaa aggaataaga tgagggaacc gcgccagcgg
     9661 cgatttaccg ccgggagccc gggttgccag ggtggtggcg attgagccac cctggcacgt
     9721 tcaccggctt gagcgttcca tagtagcgag gaacatcaag tgaacggaac agccttcgat
     9781 cttacatatt cagaatataa tagtgccgcc gcgaacgcct gatgcgacgc tttacgcgtc
     9841 ttatcaggcc tacaggacag gtcacctggt aacctacatc ttctccagca acttcttcac
     9901 atctttgcca ttgcgggaat ctttattccg ctcggcccag tcattcaggc gacgtttcgc
     9961 ttcgtcctgt agatgtttgc gcagtaactg atccacttgc aggctgtaat tgagttgctg
    10021 ccagttgcca taaacccgca gcggtactgg cgtttctttg aggaaatcaa tcagtttgct
    10081 ttccccgttc cagccaccca cgacccgaat atcaaactgg gtgtcgcagg tttgatctgc
    10141 cagattcaac atgccttccc ctgtcagcgc cagcacaggc gattgacctt gcatgtcgtt
    10201 taacgtcacg acgccatcct tcaacgtcaa atcggtggta aagcggtcaa gccgcgtcac
    10261 gttatcgaag ttttcagcgg ccttcacatc accgccatta cgctctaccg cttgctgaat
    10321 catctgctgg aagttcatcc cttccatacg tgtgtcggtc atctcaacat gcgcctgtcc
    10381 ttgccagttg tggcggaatg cgtcggcatc tatgtcagca ccggagaaat caccagccag
    10441 tgacattttt ccggtcaacg aaatcggata gttaaacgcc ttcagaatgg taccaatctc
    10501 aacgttctcc agccgtggct ggaagtttat ccgcggattt attgatgtcg cgtccagcgt
    10561 gcccggcagt gaaacctttc caccgttaag cttgccctgc agttgagtaa tttccagcaa
    10621 acccgacttg ttggtcattt gcgtggcaac atctgtaaaa ttcattccgc gccagcgcac
    10681 gttactggcc tgcaacaaaa tatcagctgt aaagccttgc agtccctgat aggccggttc
    10741 atcaatacgc gaagaaatga ccgggcgcgg caacgttgat tggctctgcc cctgctgcgc
    10801 ggcaccgttt tcaccattcg cggtttcatt aagcgggatg aggttgtcaa gattcagttg
    10861 cgggaattgc agtctcagct gccattccgg tttctctgtc agcgtgacct gtgcttgtcc
    10921 gctcagcgta ctgtcattgg cggtcaaact aatttggtta aatgaaaggc gtttatgtga
    10981 ctcctgccac tgggactgga aactcccctg cccctgaata ccttgttttg gtaaatcggc
    11041 accctgcaac tgccagttaa tttgttcaat agccgctgtt aaatcatgcg gataatcaga
    11101 agcatctacc gtaccgttaa gggatatcgt gagatcgcgc tgatcgcgat taacccgccc
    11161 ggagaactca aatgagccac gatgttgggg atcttgttcc atttgcaggc ggatattgcg
    11221 gattgtcacc tgctcgtcat cttcatgctg gaacaccaga acgctgtccg ccaccttaag
    11281 actggatata tcaaacgacc atccgcgatc gtctgacaga tccggcaagg tattgtcgcg
    11341 cggtgcaacc ggagcgtctt cactgcgcac cgcttccgtc tgcggcgtca gttggatcac
    11401 tgcccctttt agcatcacct gcttaacgct cagttgatga ctcagtagtg gtaaaagcgc
    11461 cacgtccaga cgcatgttgt cggcgcgaac cagtggctgg cttgcgccct gggcggtgag
    11521 agacattcgc ccggagagga tactaagctg cggccagacg tgccaacgca gtggcccgtc
    11581 gagctgcaat tgataaccgc tacgtgcagc aacttgcttg accatatagt cgcggaaatc
    11641 attcggattc accagcaaca ctaacgcaga taacccggcc accagcacga ccaagagtat
    11701 catcagcgtc gtcagaaatc gtctcatggt atcctcaatg ggcctgaatt agtctttatc
    11761 gattcggctg gctaccgcgc cctgctggtt gcgatatttc gcatcttcac ggcggttgta
    11821 aggtcgcgcc gccgggccgg aaagcggctc aaagctcagc gcaccaatta acatgcccgg
    11881 acgcagcgcc agcggcagct taccggagtt gtagaactcc agcacaatgc aaccagacca
    11941 gcccggatcg atacggtgcg cggtgacgtg caccatcagc cccagacgcg ccaaagagga
    12001 acgcccgtcc agccagccca ccagatcggc tggcagcgtc accgactcca gtgtcaccgc
    12061 cagcgccagc tctcctgggt gaagatagaa cgcctcgccc tcgtcgagaa cgatctcatc
    12121 gctcatcacg cggtcaagcg cggcgctcac ttcatctttg ggaccgctca gatcgataaa
    12181 cgctgccgtg tgaccacgga aggtacgaaa tttattgccc aggcgtacat ccaccgtcgc
    12241 gccgttaata cgctccactg gcggacgtgg gttgatcgac aaacggcctt catcaagcca
    12301 ggcttcaata tctcggtcac acagacgcat ggcattttct cctttcgcac atcactccct
    12361 taacgccaat tacgtcaagg gcataccaac ttatcactga acggtacaca atttatcaag
    12421 cttattcaaa gaactgactt attttcgctt tcaatatatc gatcgcgatg cggtttttcc
    12481 cgccgcgcgg cacgataatg tccgcatatt gtttagaagg ctcaatgaat tgcaggaaca
    12541 tcgggcgcac ggttttttga tattgcgcca tcacggaatc cattgaacgc ccacgctcgt
    12601 taacgtcacg cttgatgcgg cgcatcaggc agatatccag cggggtatca acgaaaatgg
    12661 agaagttaag ttcgtcacgc aaacgcgcat ccgtcagcaa caaaatgcct tcgagaatga
    12721 tgaccttctt cggctcaacc gtcaccgttt ctttcatacg cgtatgttca acatagctgt
    12781 aaaccggcag gtcaattgcc gagccgcgtt tcaacgcttg taaatgctca agcaacaggc
    12841 tgtgatccat cgcgctggga tggtcgtagt tggtcttaac gcgttcttcc atcgacagat
    12901 ggctttgatc tttgtaatag cagtcttcgg gaattacgcc gatgtgttca tcaccgactt
    12961 gctcacgtaa ttcacgataa agggtactgg caataagact cttgccggaa gccgatgcgc
    13021 cagcgatacc gataatgacg cactgatgag actgatcagt catatattta gcgacctgat
    13081 taacctggat gttaggaagg gggcgacgaa gcgccaaacg cggcaattat agggatttca
    13141 tccgtctgat accagtcgaa tagcgttgcc gcgcgctcag agttaattgt tgacaaagaa
    13201 ttcccggtgg caaattacgt tgatcagttt tatgtaaggt aaaaaatgtt atacgcagtt
    13261 gcgcaaatta tccgccttta cgtcacttta tgagcaattc gcatataaaa tgtaaaactt
    13321 ttgtactagc ataaacacag aaacgaatac tggcgacctg gccttgcgga taaagcggta
    13381 ataagcaaac aatcacagca tgtattaatt gccctgcccc acccgctgct tcacctggtc
    13441 agtttaggtt tagtctcgtt tatctttacc cttgtctcgc ttgagctttc gcagtttggc
    13501 acccaactcg ccccactgtg gttcccgacg tccatcatga tggtggcgtt ttatcgccat
    13561 gccgggcgca tgtggccggg aattgcgctg agctgttcgc tgggaaatat cgccgcatcc
    13621 attccaggaa ggccagcagg tcaaagcagg cgatttactg gcagaaattg accccagcca
    13681 gttcaaagtt gcattagcac aaacccaggg ccaactggca aaagataaag ccacgcttgc
    13741 taacgcccgc cgtgatctgg cgcgttatca gcaactggca aaaaccaatc tcgtctcccg
    13801 tcaggagctg gatgcccaac aggcgctggt cagtgaaacc gaaggcacca ttaaggctga
    13861 tgaagcaagc gtcgccagcg cgcagttgca actcgactgg agccgcatca ccgcaccagt
    13921 cgatggtcgc gttggtctca agcaagttga tgttggtaac caaatctcca gtggtgatac
    13981 caccggaatt gtggtgatca cccagacgca tcctatcgat ttagtcttta ccctgccgga
    14041 aagcgatatc gctactgtag tgcaggcgca aaaagccgga aaaccgctga tggtagaagc
    14101 ctgggatcgc accaattcga agaaattaag tgaaggcacg ctgttaagcc tcgataacca
    14161 aatcgatgcc acaaccggta cgattaaagt gaaagcacgc tttaataatc aggatgatgc
    14221 gctgtttccc aatcagtttg ttaacgcgcg catgttagtc gacaccgaac aaaacgccgt
    14281 agtgatccca acagccgccc tgcaaatggg caatgaaggc cattttgtct gggtgctgaa
    14341 tagcgaaaac aaggtcagca aacatctggt gacgccgggc attcaggaca gtcagaaagt
    14401 ggtgatccgt gcaggtattt ctgcgggcga tcgcgtggtg acagacggca ttgatcgcct
    14461 gaccgaaggg gcgaaagtgg aagtggtgga agcccagagc gccaccactc cggaagagaa
    14521 agccaccagc cgcgaatacg cgaaaaaagg agcacgctcc tgatgcaggt gttacccccg
    14581 agcagcacag gcggcccgtc gcgcctgttt attatgcgtc ctgtcgccac cacgctgctg
    14641 atggtggcga tcttactcgc cgggattatc ggttatcgcg ccctgcccgt ttcggcgctg
    14701 ccggaagtgg actatccgac cattcaggtg gtcacgctct acccaggtgc cagcccggat
    14761 gtcatgacct ctgccgttac cgcgccgctc gaacgccagt tcgggcagat gtctggcctg
    14821 aaacagatgt cgtcgcaaag ttccggcggt gcgtcagtta tcactttgca gttccagcta
    14881 acattaccgc tcaatgtcgc cgagcaggaa gtgcaggccg cgattaacgc tgcgaccaac
    14941 ttgttgccga gcgatctgcc taacccgccg gtttacagca aagtgaaccc ggcagatccg
    15001 ccgatcatga cgctcgccgt cacctcaacc gccatgccga tgacgcaagt ggaagatatg
    15061 gtggaaaccc gcgtcgcgca gaaaatctcg cagatttccg gcgtcggcct ggtgacgctt
    15121 tccggcggtc agcgtccggc tgttcgcgtc aaacttaacg ctcaggcgat tgccgccctc
    15181 ggcctgacca gcgaaaccgt gcgcaccgcc attaccggcg ctaacgttaa ctcggcaaaa
    15241 ggtagcctcg acggcccttc ccgtgcggtc acgctttccg cgaacgacca gatgcaatcc
    15301 gccgaagagt atcgccagct aatcatcgcc taccagaacg gcgcgccaat tcgtctgggc
    15361 gatgtcgcaa ctgtagagca aggtgcagaa aacagctggc tcggcgcgtg ggcgaacaaa
    15421 gaacaggcca ttgtgatgaa tgttcagcgc cagcccggtg ctaacattat ctccaccgcc
    15481 gacagcattc ggcagatgct gccacagctc actgagagtc tgccgaaatc ggtgaaggtg
    15541 acagtgcttt ccgatcgcac caccaatatc cgcgcatccg tcgatgatac tcagtttgaa
    15601 ttgatgatgg ctatcgcgct ggtagtcatg attatctacc tgtttttgcg caatattccg
    15661 gcgaccatca ttcccggtgt tgctgtaccg ctgtcgttaa tcggcacttt cgcggttatg
    15721 gtgtttctcg atttttcaat caataacctg acactgatgg ctttaactat cgccaccgga
    15781 ttcgtggtcg atgacgccat cgtggtgatc gaaaacattt cccgctatat cgaaaaaggc
    15841 gaaaaaccgt tggcggcggc gctcaagggc gcaggtgaaa tcggctttac cattatctcg
    15901 ctgaccttct cactgattgc ggtgttgatc ccactgctgt ttatgggcga tatcgtcggg
    15961 cgactgttcc gcgaatttgc tattaccctg gcggtagcga ttttgatctc agcggtggtg
    16021 tcgctgaccc tgacaccgat gatgtgcgcg cggatgctca gccaggagtc gttgcgtaaa
    16081 cagaacagct tctcccgtgc ctcggaaaaa atgttcgaca ggataatcgc cgcctatggt
    16141 cgtggactgg cgaaagtgct gaatcatccg tggctgacct taagcgtggc actcagcacg
    16201 ctgctgctta gcgtgctgct gtgggtgttc attccgaaag gtttcttccc ggtacaggac
    16261 aacggcatta ttcagggcac tttgcaggca ccgcaatcca gctcctttgc caatatggcc
    16321 cagcgacaac gccaggtcgc ggacgtgatt ttgcaggatc cggcagtgca aagcctgacc
    16381 tcatttgttg gcgttgatgg cactaacccg tcgctgaata gtgcacgttt acaaatcaac
    16441 ctcaaaccgt tggatgaacg tgatgatcgg gtgcaaaaag tcatcgcccg tctgcaaacg
    16501 gcggtagata aagtgccggg cgtcgatctc ttcctgcaac caacgcagga cctgaccatc
    16561 gatactcagg tcagccgcac ccagtaccag tttaccttgc aggccacgtc actggatgcg
    16621 ctcagtacct gggtgccaca gttgatggaa aaactccagc aactgccaca gctttctgat
    16681 gtctccagcg actggcagga caaagggctg gtggcgtatg tcaatgttga tcgcgacagc
    16741 gccagccgtc tggggatcag catggcggac gtcgataacg ccctgtacaa cgcgtttggt
    16801 cagcgactga tttccactat ttatactcag gctaaccagt atcgcgtggt gctggagcac
    16861 aataccgaaa ataccccagg cctcgcggcg ctggatacca ttcgcctgac cagcagcgac
    16921 ggtggcgtgg tgccgttaag ctcaattgcc aaaattgagc agcgttttgc gccgctttcc
    16981 atcaaccatc tggatcagtt cccggtaaca accatctcct ttaacgtgcc ggataactat
    17041 tcgctgggcg atgcggtgca ggcgattatg gacaccgaaa agacgctgaa tctgccggtg
    17101 gatatcacca cgcaattcta gggcagcacc ctcgccttcc agtcggcgct gggcagcact
    17161 gtctggctga ttgtcgcggc ggtggtggcg atgtatatcg tgctcggcat tctgtacgag
    17221 agctttattc acccgatcac cattctctcg acgctaccca ccgcaggggt cggcgcgctg
    17281 ctggcattaa tgattgccgg tagcgaactg gatgtgattg cgattatcgg cattattttg
    17341 ctgatcggta ttgtgaagaa gaacgccatc atgatgatcg acttcgcgct ggctgctgag
    17401 cgcgagcaag gcatgtcgcc gcgcgatgca atctaccagg cttgtctgtt gcgttttcgt
    17461 ccgatcctga tgaccaccct ggcggctctg cttggcgcgc tgccgctgat gttgagtacc
    17521 ggggtcggcg cggaactgcg tcgtccgtta ggtatcggca tggtcggcgg tctgattgtc
    17581 agccaggtgc tgacgctgtt caccacgccg gtgatttatc tgctgttcga ccgcctggca
    17641 ttgtggacca aaagtcgctt tgctcgccat gaagaggagg cgtaagtgaa gttttttgcc
    17701 ctcttcattt accgcccggt ggcgacgatt ttactgtcgg tcgccattac cctgtgcggc
    17761 atactgggtt tccgtatgct gccggtcgcc ccgctgccgc aggtcgattt tccggtgatt
    17821 atggtcagcg cctcgctgcc cggtgcgtcg ccggagacaa tggcgtcttc cgttgccacg
    17881 ccgctggagc gctcacttgg gcgcattgcc ggggttagtg aaatgacctc cagcagttcg
    17941 ctcggcagca cgcgcattat tttgcagttt gattttgacc gggatatcaa cggcgcagcg
    18001 cgtgatgtgc aggcggcgat caacgctgca caaagtttgc tgcccagcgg gatgcccagc
    18061 cgcccgacct atcgcaaagc gaacccgtcg gatgcgccaa ttatgatcct cacgctgaca
    18121 tccgatactt attcgcaggg tgaactgtac gatttcgcct cgacgcagct ggctccgacg
    18181 atttcgcaaa tcgacggtgt cggtgatgtc gatgtcggtg gcagctcgct gcccgccgtg
    18241 cgcgtcgggc tgaccccgca ggcgctgttt aatcagggcg tgtcgctgga cgacgtacgc
    18301 actgccatca gcaatgccaa cgtgcgtaaa ccgcaggggg cgctggaaga tggcactcac
    18361 cgctggcaga tccagaccaa cgatgaactg aaaaccgccg ctgaatatca gccgctgatt
    18421 attcactaca acaacggcgg cgcggttcgt ctgggcgatg tggcgacggt gactgactca
    18481 gtgcaggatg tgcgcaacgc cgggatgacc aacgccaaac cggctatttt actgatgatc
    18541 cgcaaactgc cggaagccaa tattatccag acggtagaca gcatccgggc aaaattacca
    18601 gagttgcagg agaccattcc ggcggcgatt gatctgcaaa ttgcccagga tcgctccccc
    18661 accattcgcg cctcgctgga agaagtcgag caaacgctga ttatctcggt ggcgctggtg
    18721 attctggtgg tctttttatt cctgcgctcg ggtcgcgcca ctattattcc cgccgttgcg
    18781 gtgccggttt cgctgattgg tacgtttgcg gcgatgtacc tgtgcggatt cagtctcaat
    18841 aatctttcgc tgatggcgct caccattgct actggtttcg tggtggatga cgccattgtg
    18901 gtgctggaaa acattgcccg tcatctggaa gcggggatga aaccactaca ggccgcgctg
    18961 caaggtactc gcgaagtcgg ctttacggtg ctgtcgatga gtctgtcact ggtggcggtg
    19021 ttcctgccgc tgctgttgat gggcggattg ccgggccgac tgttacgcga gtttgccgtg
    19081 acgctttctg tcgccattgg tatttcgttg ctggtttccc tgacgctaac gccaatgatg
    19141 tgcggctgga tgctaaaagc cagcaagccg cgtgagcaaa agcgactgcg tggttttggc
    19201 cgcatgttgg tagccctgca acaaggctac ggcaagtcgc tgaaatgggt gctcaatcat
    19261 acccgtctgg tgggcgtggt gctgcttggc accattgcgc tgaatatctc gatcccgaaa
    19321 accttcttcc cggagcagga caccggcgta ctgatgggtg ggatacaggc ggatcagagt
    19381 atttcgtttc aggcgatgcg cggcaaattg caggatttca tgaaaattat ccgtgacgat
    19441 ccggcagtgg ataatgtcac cggctttacc ggcggttcac gagttaacag tgggatgatg
    19501 tttatcaccc tcaagccacg cgacgaacgc agcgaaacgg cgcagcaaat tatcgaccgt
    19561 ctgcgcgtga agctggcgaa agaaccgggg gcgaatctgt tcctgatggc ggtacaagat
    19621 attcgcgttg gtgggcgtca gtcgaacgcc agctaccagt acacgttgtt atccgacgac
    19681 ctggcggcac tgcgcgaatg ggagccgaaa atccgcaaaa aactggcgac gttgccggaa
    19741 ctggcggacg tgaactccga tcagcaggat aacggcgcgg agatgaatct ggtttacgac
    19801 cgcgacacca tggcacggct gggaatcgac gtacaggccg ccaacagtct gttaaataac
    19861 gccttcggtc agcggcaaat ctcgaccatt taccagccga tgaaccagta taaagtggtg
    19921 atggaagtgg atccgcgcta tacccaggac atcagtgcgc tggaaaaaat gttcgttatc
    19981 aataacgaag gcaaagcgat cccgctgtcg tatttcgcta aatggcaacc ggcgaatgcc
    20041 ccactatcgg tgaatcatca gggattatcg gcggcctcga ccatctcgtt caacctgccg
    20101 accggaaaat cgctctcgga cgccagtgcg gcgatcgatc gcgcaatgac ccagcttggt
    20161 gtgccttcga cggtgcgcgg cagttttgcc ggcacggcgc aggttttcca ggagacgatg
    20221 aactcgcagg tgatcctgat tatcgccgcc atcgccacgg tgtatatcgt gctgggtatc
    20281 ctttacgaga gttacgtaca tccgctgacg attctctcca ccctgccctc ggcgggcgtt
    20341 ggagcgctgt tggcgctgga gctgttcaat gccccgttca gcctaatcgc cctgataggg
    20401 atcatgctat taatcggcat cgtgaagaaa aacgccatta tgatggtcga ttttgcgctt
    20461 gaagcccaac ggcacggtaa cctgacgccg caggaagcta ttttccaggc ctgtctgctg
    20521 cgttttcgcc cgattatgat gaccaccctg gcggcgctgt ttggcgcgct gccgctggta
    20581 ttgtcgggcg gtgacggctc ggagctgcgg caacccctgg agatcaccat tgtcggcggt
    20641 ctggtgatga gccagctcct gacgctgtat accacgccgg tggtgtatct ctttttcgac
    20701 cgtctgcggc tgcgtttttc gcgtaaacct aaacaaacgg taaccgagta aatgacagat
    20761 cttcccgaca gcacccgctg gcaattatgg attgtggctt tcggcttctt tatgcagtcg
    20821 ctggacacca ccatcgtaaa caccgccctt ccctcaatgg cgcaaagcct cggggaaagt
    20881 ccgttgcata tgcacatggt cattgtctct tatgtgctga ccgtggcggt gatgctgccc
    20941 gccagcggct ggctggcgga caaagtcggc gtgcgcaata ttttctttac cgccatcgtg
    21001 ctgtttactc tcggttcact gttttgcgcg ctttccggca cgctgaacga actgttgctg
    21061 gcacgcgcgt tacagggcgt tggcggtgcg atgatggtgc cggtcggcag gttgacggtg
    21121 atgaaaatcg taccgcgcga gcaatatatg gcggcgatga cctttgtcac gttacccggt
    21181 caggtcggtc cgctgctcgg tccggcgctc ggcggtctgc tggtagaata cgcgtcgtgg
    21241 cactggatct ttttgatcaa cattccggtg gggattatcg gtgcgatcgc cacattgatg
    21301 ttaatgccga actacaccat gcagacgcgg cgctttgatc tttccggctt tttattgctg
    21361 gcggttggca tggcggtatt aaccctggcg ctggacggca gtaaaggtac aggtttatcg
    21421 ccgctggcga ttgctggcct ggtcgcagtt ggcgtggtgg cactggtgct ttatctgctg
    21481 cacgccagaa ataacaaccg cgccctgttc agtctgaaac tgttccgcac tcgtaccttt
    21541 tcgctgggcc tggcggggag ctttgccgga cgtattggca gtggcatgtt gccctttatg
    21601 acaccggttt tcctgcaaat tggcctcggt ttctcgccgt ttcatgccgg actgatgatg
    21661 atcccgatgg tgcttggcag catgggaatg aagcgaattg tggtacaggt ggtgaatcgc
    21721 tttggttatc gtcgggtact ggtagcgacc acgctgggtc tgtcgctggt caccctgttg
    21781 tttatgacta ccgctctgtt gggctggtac tacgttttgc cgttcgtcct gtttttacaa
    21841 gggatggtca actcgacccg tttctcctcc atgaacaccc tgacgctgaa agatctcccg
    21901 gacaatctgg cgagcagcgg aaacagcctg ctgtcgatga ttatgcaatt gtcgatgagt
    21961 atcggcgtca ccatcgccgg gctgttgctg gggctttttg gttcgcagca tatcagcgtc
    22021 gacagcggca ccacacaaac cgtctttatg tacacctggc ttagcatggc gtttatcatc
    22081 gcccttccgg cgttcatctt tgccagagtg ccgaacgata cgcatcaaaa tgtagctatt
    22141 tcgcggcgaa aaaggagcgc gcaatgaagt tctggcgacc cggtattacc ggcaaactgt
    22201 ttctggcgat tttcgccacc tgcattgtct tgctgatcag tatgcactgg gcggtgcgta
    22261 tcagttttga gcgtggcttt attgattaca tcaaacacgg caatgaacag cgattacagc
    22321 tgttaagtga tgcgcttggc gagcagtatg cgcagcatgg taactggcgc ttcctgcgca
    22381 acaacgatcg ctttgtcttt cagatcctgc gttcatttga acacgataat tcggaagata
    22441 aacccggccc gggtatgccg ccgcacggct ggcgtaccca gttctgggtg gtcgatcaaa
    22501 acaacaaagt actggttggt ccgcgagcgc cgattccacc tgacggtaca cggcgaccca
    22561 ttctggtcaa cggtgcggaa gtgggcgcgg tgattgcctc ccctgttgag cggctgaccc
    22621 gcaatactga tatcaatttc gataaacaac agcggcaaac cagctggctg atcgtcgccc
    22681 tggcaacgtt actcgcggcg ctcgccacct ttctgctggc gcgcggtttg ctggcaccgg
    22741 taaaacgact tgtcgatggc acgcacaaac tggcggcggg cgatttcact acccgcgtaa
    22801 cgcccaccag tgaagatgaa ctgggcaaac tggcgcaaga cttcaaccag ctcgccagca
    22861 cgctggagaa aaaccagcaa atgcggcgcg attttatggc cgatatttct cacgaactgc
    22921 gtacgccatt agcggtgctg cgcggtgaac tggaagccat tcaggatggc gtgcgcaaat
    22981 tcacgccgga gacggtggcg tctttacagg cggaggtcgg tacactgacc aaactggttg
    23041 acgatcttca tcagttgtcg atgtctgatg aaggcgctct cgcctatcaa aaagcaccgg
    23101 tagatttgat cccactgctg gaagtggcgg gcggcgcatt tcgcgaacga ttcgccagcc
    23161 gtggcctgaa actgcaattt tccctgccag acagtatcac cgtatttggc gatcgcgacc
    23221 gtttaatgca gttattcaat aacttactgg aaaacagcct gcgctacact gacagcggcg
    23281 gcagcctgaa aatctctgcc gagcagcacg acaaaacggt gcgcctgacc tttgccgaca
    23341 gtgcgccagg tgtcagtgac gatcagctac aaaaattgtt tgaacgtttt tatcgcaccg
    23401 aaggttcccg caaccgcgcc agcggcggtt ccgggctggg gctggcgatt tgcctgaaca
    23461 ttgttgaagc ccataatggc cgcattattg ccgcccattc gccttttggc ggggtaagca
    23521 ttacagtaga gttaccgctg gaacgggatt tacagagaga agtatgaccg agttaccaat
    23581 cgacgaaaac acaccgcgta ttttgatcgt ggaagatgaa ccgaagctgg ggcagttgct
    23641 cattgattat ctgcgtgctg cgagctatgc gccgacgctt atcagccacg gcgatcaggt
    23701 actggcgtat gtgcgccaga caccaccgga tctgatcctg ttggatctga tgctccctgg
    23761 caccgatggc ctgacattat gccgggaaat tcgtcgtttt tccgatgttc cgatcgtgat
    23821 ggtgacggca aaaatcgaag agatcgaccg cctgctgggg ctggagattg gcgccgatga
    23881 ctacatctgc aagccgtaca gcccacggga agtggtagcg cgtgtcaaaa ccattttgcg
    23941 ccgttgtaaa ccgcaacgcg aacttcagca acaggatgcc gaaagcccgt tgattatcga
    24001 cgaaggtcgt tttcaggctt catggcgcgg taaaatgctt gacctgacgc ctgcggaatt
    24061 tcgtctgctt aaaacgctct ctcacgaacc aggaaaagtg ttctcccgcg agcaattgct
    24121 caatcatctt tatgacgact accgcgtagt aaccgaccgc accattgaca gccacattaa
    24181 aaatctgcgc cgcaagctgg aatctctcga cgccgaacag tcatttatcc gcgccgttta
    24241 cggcgttggt taccgctggg aagccgacgc ctgccgcatc gtttagtttt agcgacatta
    24301 ctttgttagc cggagatggc gttcagaccc gatccggcaa cattattcca cgcatggtca
    24361 gcaaactgac attatctctc cgccctggca ggcatcagaa aatcgctcat actttaatca
    24421 gtaaacagca cctttagatg ctgttttcga taagcaattt caatcaagga gtcattatgg
    24481 ctggttggtt tgaactcagt aagagcagtg ataatcagtt ccggtttgtg ctaaaagcgg
    24541 gcaatggtga gactatcctc accagcgagc tgtatacctc aaaagcctct gcggaaaagg
    24601 gcatcgcgtc ggtgcgtagc aacagcccgc aagaagagcg ctatgagaaa aaaacggcaa
    24661 gcaacggcaa attctatttc aatctgaaag ccgctaatca tcaaattatc ggctccagcc
    24721 agatgtacgc caccgcgcaa tctcgtgaaa ccggaattgc ctccgttaaa gccaatggca
    24781 caagccagac ggtgaaagac aatacgtaat tacgatgccg ggcgtgggtt gttggcgtcc
    24841 ggcgctttac cagatccccc ctacagcgct acaatgcccg cccttaaagt gggggcctcc
    24901 cctaaccgct tcatcaggtg aagcggatct gacctgtcat cagaacgaga gaattatgtt
    24961 taaaccggaa ctcctttccc cggcgggaac gctgaaaaat atgcgttacg ctttcgctta
    25021 tggcgcagat gctgtttatg cgggccagcc gcgttactcc ctacgtgtgc gcaacaacga
    25081 attcaaccac gaaaatcttc agctcggcat caatgaagcc cacgcgctgg ggaaaaagtt
    25141 ttatgtcgtg gtcaacattg caccgcacaa cgccaagctg aaaaccttta tccgtgacct
    25201 gaaaccggtg gtggaaatgg ggccggatgc gctgattatg tccgatccag ggctgattat
    25261 gctggtgcgt gagcacttcc ctgaaatgcc gatccacctc tcggtgcagg ctaacgccgt
    25321 gaactgggcg acggtgaaat tctggcagca aatgggactg acccgcgtga tcctctctcg
    25381 cgagctgtcg ctggaagaga ttgaagagat ccataatcag gtgccggata tggagatcga
    25441 gatcttcgtt cacggcgcac tgtgcatggc ctactccggt cgctgcctgc tctctggcta
    25501 tatcaacaag cgcgacccga accagggcac ctgcaccaac gcctgccgct gggagtacaa
    25561 cgtccaggaa gggaaagaag atgacgttgg caacatcgta cacaagtacg agccgattcc
    25621 ggtgcaaaat gttgagccga cgctgggtat cggcgcgcca accgacaaag tgtttatgat
    25681 cgaagaagcc cagcgtccgg gcgagtatat gaccgcgttt gaagatgagc acggcactta
    25741 catcatgaac tcgaaagatc tgcgcgccat cgcccatgta gaacgcctga ccaaaatggg
    25801 cgtgcattcg ctgaaaatcg aaggtcgtac caaatctttc tactattgcg cacgcaccgc
    25861 acaggtttac cgcaaagcta tcgatgacgc cgctgcggga aaaccgttcg ataccagcct
    25921 gctggaaact ctggaaggtc tggcgcatcg tggctatacc gaaggtttcc tgcgtcgtca
    25981 tactcacgac gattatcaga actacgaata cggttattca gtttctgacc gccagcagtt
    26041 tgttggtgag tttaccggtg agcgcaaggg ggacctcgca gcggtagcgg tgaaaaataa
    26101 attctccgtt ggcgacagtc ttgagctgat gacgccgcaa ggcaacatta actttaccct
    26161 tgagcacatg gaaaacgcca aaggcgaagc tatgccggtc gcaccaggcg atggttatac
    26221 tgtgtggctc ccggtcccgc aggatcttga gctaaattac gcgctgctaa tgcgtaattt
    26281 ctccggggaa accacgcgta acccccacgg taagtgatta atttcgatta tttttcccgg
    26341 atggaaaatt cttagaaacc gatcacatac agctgcattt attaaggtta tcatccgttt
    26401 cgctgaaaaa cataatccat aaaatgctag ctgtaccagg aaccacctcc ttagcctgtg
    26461 taatctccct tacacgggct tattttttgt acataagctg taaagaagga ttgatccctg
    26521 ataaccgtct tccattgacc acatcgatag aatccccctt catagcacaa tgcctttcac
    26581 gtaacggtat cgtgctcgcc caggttccgg ctacgcacag ccagaacgcg catgtttgac
    26641 gcttacaaaa aaatattctc actctccaca tttgaatgtc agacgagcga cgccatgtaa
    26701 tcctgcacct tctgtcttca ggtcaactat ctgcattttt ttgccctgag taacacagaa
    26761 atgggctgca tcatttttta ctatattttc tgcaccagat attctgcccc tggctaaaga
    26821 agcttcggct tcggtgtagt attggttatc gagtttacgc tgaatattac ttttatatgc
    26881 aagaccaaat ttaccgatac ttgtttcatc attatgcaca gcacaaccag atataagaaa
    26941 aatactaatt aatgatatag cagctatctt tttcatctca ccttccccca ttaaatacca
    27001 acgacactct ctagtgttta aatataataa tggcatgatt attataattg aataggatta
    27061 taataaatgt tctgtacaac atttcctaca taagtaggaa ttacggacat tgaggccctt
    27121 cagggtaact ccatgggggc tttaatatat tatattgaag atgccactgt ttagttgaat
    27181 attaggtata tgctcttttt tgaaatttat cggtggcagc cgttagtatt cgctgtcccc
    27241 attgcaagct cctggtggta accactgaat cctccatact tgaactgact ttttatcctc
    27301 cgactttcat cctgttctga ctccaccttt tgttttctgc tctacactat ctacagacca
    27361 atcataaagg cacatacgat catggcagaa tttcccgcca gcttactgat tcttaatggt
    27421 aaaagtactg acaatctacc cttgcgcgaa gcaattatgc tgttgcgtga ggaaggaatg
    27481 acgatccatg tgcgggtcac ctgggagaaa ggtgatgccg cacgatttgt agaggaggcc
    27541 cggaagttgg gcgtcgcaac ggtgattgcc ggtggtggcg atggcaccat taatgaagtt
    27601 tctacggcgt tgattcagtg tgagggggat gacatacccg cgctgggaat tttgccatta
    27661 ggaaccgcca atgattttgc caccagtgta gggattcctg aggcactgga taaggcgctg
    27721 aaactggcaa ttgccggtaa tgccattgcg atagatatgg cgcaggtcaa caaacaaacc
    27781 tgttttatta atatggcgac aggcggattt gggacgcgta ttaccacaga aacgccggaa
    27841 aaattaaaag ccgcgctggg tggcgtctct tacatcattc atggcttaat gcgcatggat
    27901 actctgcaac cggaccgttg tgaaatccgc ggtgaaaact ttcactggca aggtgacgcc
    27961 ctggtcattg gtattggtaa cgggcgtcag gccggtggcg gtcaacaatt gtgcccgaac
    28021 gcgttaatta acgatggctt gctgcaactg cgcattttta ccggcgatga aattcttccg
    28081 gctctcgtat caaccttaaa atctgacgaa gataacccga atattatcga aggcgcttcg
    28141 tcgtggtttg atatacaagc cccacacgaa atcactttta atcttgatgg cgaaccgttg
    28201 agtgggcaaa acttccatat tgaaatactt ccggcggcgt tgcgttgtcg attaccacca
    28261 gattgtccat tattgcgtta atcaaactat tctctcttga accagggaga atccgtaatg
    28321 ccggatgcgg tgtaaacacc ttatccggca tacagaacaa tactacaccg taattaattt
    28381 tacccgtgtc ttctgcaatg ccaatttatc cacctctgac acagcatcat cggtaacaat
    28441 aatgtcaatt ttttcgattg gcagtacctg attaaaaccg cgacgattaa atttcgatga
    28501 atcgagaacg gcaatcactt tatttgcggc ggtgaccatc gccccgctaa tcgaataacc
    28561 ttcattaaag gtcgtaatac cattaaccgc atcaatacca tcagcaccga caaacattaa
    28621 atcggcatta atatcctgca atgaacgctc ggcaatggaa ccatgcatcg agcgcgtttt
    28681 atggcggacc gtgccaccac agacgaccag agtaatgtct ttattttcgg aaagggcaaa
    28741 cgctgccggg agactgttgg taatcaccgt gatattttta gcggtcatta atccttcggc
    28801 gataagcatt gtggtacttc cgctgtcgag aataacggtc atcccttcat ggatcatttt
    28861 gactgccgcc tgcgctattc tgttttttgg cgcgctggcg agctgaaatc gctctttaaa
    28921 cccgacttcc tgggtctcgg tttcactatt accggacatt attttcgccg caccgccatg
    28981 aaagcgcgta accacgcctt tttgttcgag aaagcgcaaa tcggcacgga ttgtcgcttc
    29041 cgaggcagca aatactcccg ccagatcctg aacaagcacg gttccctgtt cattcactaa
    29101 ttggatgatc ttattccttc gctcgaatga attcatcgtc atttacccat attcaattgt
    29161 ggctagtgta aacgaagggg gaccattggt gaatgatctg ttttgcacaa taccgggcca
    29221 gcatcacgct ggcccgcggt ttcatggaat gagcaacact ttgccctgca tggcattacg
    29281 agcgatgtca cgcaccgcct gggtgaagct ttcaaagctt ccacggtgag cgattaatgg
    29341 ctccaggctt aacttacgtt ctgtcagcaa ccggctcgcc gtttcccact cctgccccgg
    29401 ccaagggctg gaatagttca tccaactgcc gataaccgtc agctctttac gcaatatttt
    29461 gccaaacgtt gccgatgtta aatgcagatc ctgatgcaac gtgcccacca gcgccagttg
    29521 ggcatgagga ccggcaatct ctaccgccag ttcgacagtt tgcggtacgc cagccgtctc
    29581 gaggataagc tgattaaagc gcagttcgcg taaaacgctc tgcatttgcg gcgcgctcat
    29641 ttcgctactg ttaaatgttt gcatcgcacc gaaagatttt gccagtgcca gtttttctga
    29701 gctaatgtcg atcgccgtca cactctttgc tcccagcgcg acagcgcact gaatagccag
    29761 caggccaatg gttccggcac caataataat aacgttttta ttctcacaac cttgcgctaa
    29821 atgaaaagca tgcaggccaa cggtaatcgg ctcaataaaa gccccatcct caataggcat
    29881 atccgtgggt agagcaaaga cattttttcg cttaacgaca atatattcag caaatccacc
    29941 atcacgccgc gagccaataa aatcatattt tgcgcactgg gaataaaacc ctttcaaaca
    30001 ctctggacaa gtaaaacagg gtaataacgg cacacaggca accgcatcgc cagggtgtaa
    30061 atcatcaaca ccggatccca ccgcatcaat atagccgcta aattcatggc ctaacgttat
    30121 tggataataa tgtgcaccat ttttaaatat cctgggtaaa tcggaaccac ataagcccga
    30181 gctggcaatt tttacccgca cctcatcctg atgtttaatt tcaggaatga cgctttttgc
    30241 aacgcgcacg ataccatcag tatcattcac tactgatttc ataaaaactc ctgat
//
LOCUS       DAJTSA010000041        30012 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000041 DAJTSA010000000
VERSION     DAJTSA010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.041"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OO369_002012"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OO369_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR5982291.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OO369_002013"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OO369_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR5982292.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="OO369_002014"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="OO369_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR5982293.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="OO369_002015"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="OO369_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR5982294.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="OO369_002016"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="OO369_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR5982295.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="OO369_002017"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="OO369_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR5982296.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="OO369_002018"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="OO369_002018"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR5982297.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="OO369_002019"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="OO369_002019"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR5982298.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO369_002020"
     CDS             complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO369_002020"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR5982299.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO369_002021"
     CDS             complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO369_002021"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR5982300.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO369_002022"
     CDS             complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO369_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR5982301.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO369_002023"
     CDS             complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO369_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR5982302.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO369_002024"
     CDS             complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO369_002024"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR5982303.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO369_002025"
     CDS             complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO369_002025"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR5982304.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18150..18250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO369_002026"
     CDS             complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO369_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR5982305.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18874..19647
                     /gene="nudC"
                     /locus_tag="OO369_002027"
     CDS             18874..19647
                     /gene="nudC"
                     /locus_tag="OO369_002027"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR5982306.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19687..20751
                     /gene="hemE"
                     /locus_tag="OO369_002028"
     CDS             19687..20751
                     /gene="hemE"
                     /locus_tag="OO369_002028"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR5982307.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20908..21579
                     /gene="nfi"
                     /locus_tag="OO369_002029"
     CDS             20908..21579
                     /gene="nfi"
                     /locus_tag="OO369_002029"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR5982308.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21622..22212
                     /locus_tag="OO369_002030"
     CDS             21622..22212
                     /locus_tag="OO369_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR5982309.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22399..22671
                     /gene="hupA"
                     /locus_tag="OO369_002031"
     CDS             22399..22671
                     /gene="hupA"
                     /locus_tag="OO369_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR5982310.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22684..23379
                     /locus_tag="OO369_002032"
     CDS             22684..23379
                     /locus_tag="OO369_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR5982311.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO369_002033"
     CDS             complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO369_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR5982312.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24044..25420
                     /gene="zraS"
                     /locus_tag="OO369_002034"
     CDS             24044..25420
                     /gene="zraS"
                     /locus_tag="OO369_002034"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR5982313.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25417..26742
                     /gene="zraR"
                     /locus_tag="OO369_002035"
     CDS             25417..26742
                     /gene="zraR"
                     /locus_tag="OO369_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR5982314.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO369_002036"
     CDS             complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO369_002036"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR5982315.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO369_002037"
     CDS             complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO369_002037"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR5982316.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7029 a         7822 c         8255 g         6906 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cattgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggcagg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgtgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgt ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccaa gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ttggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaatatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacattgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaat ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag gggggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatattctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12601 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    12661 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    12721 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    12781 acggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    12841 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    12901 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    12961 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13021 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13081 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13141 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13201 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13261 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13321 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13381 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13441 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13501 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13561 ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13621 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    13681 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    13741 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    13801 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    13861 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    13921 gcaggcctac ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    13981 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgtggcat
    14041 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14101 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14161 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14221 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14281 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14341 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14401 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14461 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14521 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14581 acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    14641 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    14701 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    14761 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    14821 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    14881 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    14941 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15001 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15061 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15121 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15181 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15241 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15301 cgggctgttg atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15361 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15421 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15481 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15541 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15601 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    15661 cgcgtgccag actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat
    15721 gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    15781 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    15841 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    15901 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc
    15961 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16021 catcggcttc cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc
    16081 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16141 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat
    16201 ttctccgcct ctggcacgga agttctccga catatccgcc attcccactt caatggtttg
    16261 cgcggcggcg taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca
    16321 catggagcaa aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc
    16381 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16441 ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc
    16501 agcgtgggcg gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag
    16561 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16621 cgccgcacca atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg
    16681 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg
    16741 gatcatctgc atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca
    16801 ggcgattttg gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc
    16861 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    16921 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    16981 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17041 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17101 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17161 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa
    17221 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17281 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17341 cgagttgccg atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc
    17401 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17461 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17521 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17581 ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    17641 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    17701 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg
    17761 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    17821 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    17881 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    17941 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18001 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18061 tgcagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca
    18121 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18181 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18241 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18301 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18361 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18421 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18481 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18541 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18601 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    18661 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    18721 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    18781 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    18841 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    18901 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    18961 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19021 cagggggaac ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt
    19081 caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg
    19141 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19201 accgaatggg cgatgctgtg tagccattgc cgtgagcgtt actacccgca aatcgccccc
    19261 tgcattattg ttgccatccg tcgcgatgat tcgctcctcc tcgcccagca tacccgccat
    19321 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19381 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19441 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19501 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19561 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19621 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    19681 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    19741 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    19801 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    19861 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    19921 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    19981 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20041 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa
    20101 gtgccgctga ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc
    20161 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20221 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20281 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
    20341 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20401 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
    20461 atggctgaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
    20521 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20581 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    20641 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    20701 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat
    20761 ccatcaggct attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact
    20821 acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc
    20881 tgttgaacca ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc
    20941 gaactggctt cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc
    21001 gccggagccg atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg
    21061 ctgaaatatc cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg
    21121 ccttacattc caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag
    21181 atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc
    21241 cgtcttggcg tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg
    21301 aaaaaacggc tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca
    21361 ctgatggata aaggcgagca gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg
    21421 ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc
    21481 tgcatgaaag gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa
    21541 cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat
    21601 ttccgtattt gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg
    21661 gaaagctggc agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac
    21721 gccatgttct gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat
    21781 ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag
    21841 aaatttgaag aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca
    21901 atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc
    21961 gaacacgcgg tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact
    22021 caggctggtc gcgaaatggg cgatgaagag cttaaagaaa acccagctgt agagcaagaa
    22081 tgggacattc agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag
    22141 ctgataaaag gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt
    22201 tttcagcaat aagaccagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc
    22261 cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg
    22321 tatgcaggag agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac
    22381 acattgtaag gataacttat gaacaagact caactgattg atgtaattgc agagaaagca
    22441 gaactgtcca aaacccaggc taaagctgct ctggagtcca ctctggctgc aattactgag
    22501 tctctgaaag aaggcgatgc tgtacaactg gttggtttcg gtaccttcaa agtgaaccat
    22561 cgcgctgagc gaactggccg caacccgcag accggtaaag aaatcaaaat tgccgcagct
    22621 aacgtaccgg catttgtttc tggcaaggca ctgaaagacg cagttaagta agattgcgtg
    22681 gcagtgaaca gttttaacga aggggtggtt tcaccccttt tgtctttctg gcgtcgatca
    22741 ttgatgctgg caggtgcgct gtttctcact gcctgtagcc acaactcttc acttcctccc
    22801 tttaccgcca gtggatttgc tgaagaccag ggcgcggtac gcatctggcg aaaagacagc
    22861 ggcgataatg tgcatctgct tgccgtgttt agcccgtggc gcagtggcga taccacgacg
    22921 cgagagtatc gctggcaggg cgataacctc acgctcatca atatcaatgt ttacagcaaa
    22981 ccgccggtga acattcgcgc gcgttttgac gatcgcggcg atctgagctt tatgcaacgt
    23041 gaatccgatg gggaaaagca gcagctttct aacgaccaaa tcgatttata ccgttatcgt
    23101 gctgatcaga tccgccagat tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag
    23161 gggcgctggc atgcgatgga acagaccgtg accacctgcg aagggcaaac cattaaacct
    23221 gatttagatt cgcaggcgat agcgcatatc gagcgccgcc agagccgctc ttctgttgat
    23281 gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat tactgttagt ggcaaactct
    23341 gatttctgtc gctggcaacc caacgagaaa acgttctgat ttaccagtgg cccataccca
    23401 tatgaccgcc accaccgcag ccgccgtagc ccatgcccat tccggctccg cgcggaatac
    23461 ccgcttcagc catcgcgata tctcgtttca cccgtaactc atctaacgac tgacgcaaat
    23521 tctccatctc tttggcgacc gcgttaattt tgctgctatc cggtgggttc gcggctaaca
    23581 gggcattgta ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt
    23641 aaaagtcatt atggattttc tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat
    23701 tttgctgcca cataccgtgt ccgccgtgag caaatgcaga tgtcgatccc atcgccattg
    23761 ctgaaagcgc catcattacc agggcaattt tcgtgttccg tttcatggtt aatcctccag
    23821 tggttgtctt acttcgggta ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg
    23881 gtaatctggc atgataaacg agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt
    23941 tactcattga aacttaagcc tttgtgttac agcgcagggt aagcgctgat aaaagatggc
    24001 atgatttctg ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg
    24061 ttctaaagac tccttagcta aatggttaag cgcgatcctc cccgtggtca ttgttgggct
    24121 ggtagggttg tttgcggtga cggtgattcg cgattatggg cgcgagactg ccgccgccag
    24181 acaaacgctg ctggaaaaag gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt
    24241 cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc ttactggaag aaatggccgg
    24301 gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag
    24361 caactccggc atggtgggaa aacagcttta ttcccctcag gaaatgcagc agttacatcc
    24421 gggagatgaa gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg ttctggaaat
    24481 ttatcgccag tttcaaccga tgtttgctgc tggaatgtac cggatgcgcc atatgcagca
    24541 gtatgccgcg acaccacaag caattttcat cgctttcgac gccagtaata ttgtgagtgc
    24601 cgaagatcgt gagcagagaa acaccctgat tattctcttc gccctggcga cggtcttgct
    24661 ggcaagcgta ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc agcttctaca
    24721 agatgaaatg aagcgcaaag agaagctggt ggcgctgggg catcttgcgg caggcgttgc
    24781 ccacgaaatc cgtaacccac tttcctcgat taaaggactg gcgaaatact ttgccgagcg
    24841 cgcgcctgca gggggagaag cgcatcaact ggcgcaggtg atggcgaaag aggccgaccg
    24901 tttaaaccgc gtggtaagcg agttgctgga actggttaag ccaacgcatc tggctttgca
    24961 ggcggtggat ctcaacacgc tgattaacca ctcattacag ctggtaagcc aggatgcaaa
    25021 cagccgggag atccagttac gctttaccgc caacgacaca ttaccggaaa ttcaggccga
    25081 tccggacagg ctgactcagg tcctgttgaa tctctatctc aatgctattc aggcgattgg
    25141 tcagcatggc gtgattagcg tgacggccag cgaaagcggc gcgggcgtga aaatcagcgt
    25201 taccgacagc ggtaagggaa ttgcggcaga tcagcttgaa gccatcttca ctccgtactt
    25261 caccaccaaa gccgaaggca ccggattggg gctggcggtc gtgcataata ttgttgaaca
    25321 acacggtggt acaattcagg tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg
    25381 gcttccggtc aatattacgc gtaaggaccc acaaggatga cgcacgataa tatcgatatt
    25441 ctggtggtgg atgatgacat tagccactgc actattttgc aggctttact gcgcggctgg
    25501 ggctataacg tcgcgctggc gaacagcggg cgacaggcgc tggagcaggt gcgggaacag
    25561 gtttttgatc ttgtgctttg cgatgtgcga atggcggaga tggacggcat cgccacgctg
    25621 aaagagatca aagcgttaaa cccggcaatt ccggtgctga ttatgactgc gtactccagc
    25681 gtcgagacgg cggtagaggc actgaaaact ggggcgcagg attatctcat caagccgctg
    25741 gatttcgata acctacaggc gacgctggaa aaggcgctcg cgcatacgca cagtattgat
    25801 gctgaaacac ctgcggtgac tgccagccag ttcggtatgg tcggtaaaag cccggcgatg
    25861 caacacctgc tcagtgaaat cgccctcgtc gcgccatcgg aagccacggt gctgatccac
    25921 ggcgattccg gcaccggtaa agagctggtc gccagggcga ttcacgccag tagcgcacgt
    25981 agcgaaaaac cgctggtaac gctcaactgt gcggcactca acgaatcctt gctggaatct
    26041 gaattgttcg gtcacgaaaa aggggcgttt actggagccg ataaacgccg ggaagggcgc
    26101 tttgttgagg cggacggcgg cacgctgttt ctcgatgaaa ttggcgatat ctcgccgatg
    26161 atgcaggtgc gtctgctgcg tgcgattcag gagcgcgaag ttcagcgtgt cggtagcaac
    26221 cagactatct cggttgatgt ccggctgatt gcggcgaccc atcgcgatct tgccgcagag
    26281 gtgaatgccg ggcgttttcg ccaggatctc tactatcgcc tgaatgtggt ggcgattgaa
    26341 gtaccatcgc tgcggcaacg gcgggaagat atccctctgc tggctggcca ttttcttcag
    26401 cgctttgccg agcgtaatcg caaggcggta aaaggcttta cgccacaggc gatggatttg
    26461 ctgatccact acgactggcc gggaaatatt cgtgaactgg aaaacgcggt ggaacgggcg
    26521 gtggtgctgc tgaccgggga atatatttcc gaacgcgagc tgccgctggc gattgccagt
    26581 acgccgatcc cgctggggca aagtcaggat attcagccgc tggtggaagt ggaaaaagag
    26641 gtgattctgg cggcgctgga gaaaacgggc ggcaacaaaa ccgaagccgc ccgtcagtta
    26701 gggatcacgc gcaaaacgct attggcaaaa ctgtcgcgtt agttctgctc gcgttcgatg
    26761 gcgcgccaac cgatatcttt ccggcagaag caatcatccc agtggatatc ggtcattaag
    26821 gcgtaggcgc gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac gcacagtacg
    26881 cgcccgccgc tggttaccac ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact
    26941 ttgccgtcag ccacttcttc cagcggcagg ccgtggatca catcgccggt gcggtaatcg
    27001 cccggatatc cacccgcagc catcaccacg ctgagagaag cacgttcatc ccactctgac
    27061 gttttctcgt ccagtttgcc ttcacaggcc gccaggcaga gctcaaccag atcggacttc
    27121 atgcgcagca taatcggctg ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc
    27181 ttcggattgc cctgtttgtc gatcatcagg cccgcataga gaaaaccggt gtaggtgttg
    27241 ccttccgccg ccatgccttt cacggttggc cagatgatac gttccatggt gcgctgatga
    27301 acttcatcgg ttactaccgg cgcgggggag taagcgccca tcccgccggt gtttggtccg
    27361 ttatctttat cgcctacgcg tttgtgatcc tggctggtag ccatcggcag cacatgctcg
    27421 ccgtccacca tcacgataaa gctcgcttct tcgccatcga ggaactcttc gataacgatg
    27481 cgatgacccg cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc cgcttccgct
    27541 tcttccagcg tcatcgccac gataacgcct ttcccggcag ccagaccgtc cgctttaatg
    27601 acgattggcg cgcctttctc acgcagatac accagcgcag gttctacctc ggtgaagttc
    27661 tggtattccg ccgtagggat cttatggcgg gccaggaaat ctttggtaaa cgcttttgag
    27721 ccttccagtt gggccgcacc tgcggttggg ccgaagattt tcagcccggc ggcgcggaag
    27781 gtatcgacca cgcctttcac cagcggcgct tccgggccga cgatggtcag atcaatcttt
    27841 tcgttttgtg cgaaatccaa cagcgccggg atatcggtca cgccgatagc aacgttttgc
    27901 agcgcgggtt ccagcgcagt gcctgcatta cccggagcag caaaaacagt ctcaaccagc
    27961 ggcgactggg ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat
    28021 actttcatct attgctccat taatggcgga agtggcgcat gtcggtgaag agcatcgcaa
    28081 taccgtgctc gtcggcggcg gcaatcactt cgtcatcacg gatggaaccg ccaggctgga
    28141 ttacgcaggt cacgcccgca gcggcggcgg catcaatacc gtcgcggaac gggaagaacg
    28201 cgtcagaagc catcgaggaa cctttcactt ccaggccttc atcggcagct ttaataccgg
    28261 cgattttcgc ggagtacacg cggctcatct ggcccgcgcc aataccgatg gtcatattgt
    28321 ttttggcata gacgatagca ttggatttca cgaatttcgc cactttccag cagaacagcg
    28381 catcacgcag ttcctgttcg gtcggctgac gttgggtgac gacgcgcagt tcttccgcac
    28441 cgaccatccc caggtcacga tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa
    28501 gacccggaac acgctcgccc cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg
    28561 cggtgatttt cagggcttct tcgctggcgg aaggcgcaat aatcacttcg acaaactgac
    28621 gagaaatgat ggcctgtgcg gtttccgcat ccagctcgcg gttaaaggca atgatgccgc
    28681 cgaatgcgga ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga atggaattgc
    28741 cgatagctac accgcaaggg ttggcgtgct tcacaatcac acatgccggc tcggcgaact
    28801 ctttcacgca ctccagcgcc gcatcggtat cggcgatgtt gttataagag agggctttac
    28861 cctgaacctg ggttgcggta gcaacggagg cttctttcac attctcttct atatagaagg
    28921 cagcctgctg gtggctgttc tcgccgtaac gcatatcctg cttcttaatg aagttcaggt
    28981 tcagcgtgcg tgggaagcga ccggcggctt ctttgctttc accgtggtac gccggaacca
    29041 tgctgccgaa gtagttggca atcatgctgt cgtaggcggc agtgtgttcg aaggctttga
    29101 tggcgaggtc gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc atctctttaa
    29161 taatggcgtc gtagtcgctg ctcttcacca cgattgcgac atctttatgg ttcttagcgg
    29221 cggagcgcac catggtcggg ccgccgatat cgatgttctc aaccgcatct tccagcgagc
    29281 agccttcacg agcaacagtc tgggcgaacg gatacaggtt aacaaccacc atatcgatag
    29341 gctggatctg atgttcttcc ataatggcat cgtcctggcc gcgacggccc agaatgccac
    29401 catgtacttt cggatgcagg gtcttcacgc gtccatccat catctccggg aaaccggtgt
    29461 aatcggaaac ttcggttacc ggcagacctt tttctgctaa cagacgggca gtgccccctg
    29521 tagacagcag ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt
    29581 tgtcagaaac actgagcaga gcgcggcgga ctggacgacg ttgttgcatg gtaaatcccc
    29641 tgggtttgac tattacagag agcgttagct gaatttttcg cgaaaaactc agctaacgcc
    29701 cctaacgggg catccttatt tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg
    29761 cgaatttttc tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac
    29821 agaaattaca ttgatgattg tggataactc tgtgcgtaaa aaggtataaa gcggggtttt
    29881 gctggggaat gcagcagtca gtcatttttc tgcaattttt ctattgcggc ctgcggagaa
    29941 ctccctataa tgcgccttca tcgacacggc ggatgtgaat cacttcacac aaacagccgg
    30001 ttcggttgaa ga
//
LOCUS       DAJTSA010000042        29797 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000042 DAJTSA010000000
VERSION     DAJTSA010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29797
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.042"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            56..850
                     /locus_tag="OO369_002038"
     CDS             56..850
                     /locus_tag="OO369_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5982317.1"
                     /translation="MKIFKYTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLY
                     DAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETW
                     FNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDI
                     TMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVH
                     HHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            872..2281
                     /locus_tag="OO369_002039"
     CDS             872..2281
                     /locus_tag="OO369_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5982318.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2259..3038
                     /locus_tag="OO369_002040"
     CDS             2259..3038
                     /locus_tag="OO369_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR5982319.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3035..3895
                     /locus_tag="OO369_002041"
     CDS             3035..3895
                     /locus_tag="OO369_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR5982320.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4043..4618)
                     /locus_tag="OO369_002042"
                     /pseudo
     CDS             complement(4043..4618)
                     /locus_tag="OO369_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4635..4895)
                     /locus_tag="OO369_002043"
     CDS             complement(4635..4895)
                     /locus_tag="OO369_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5982321.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4886..6157)
                     /locus_tag="OO369_002044"
     CDS             complement(4886..6157)
                     /locus_tag="OO369_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5982322.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6235..6597)
                     /gene="queD"
                     /locus_tag="OO369_002045"
     CDS             complement(6235..6597)
                     /gene="queD"
                     /locus_tag="OO369_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR5982323.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6916..8715
                     /gene="cysJ"
                     /locus_tag="OO369_002046"
     CDS             6916..8715
                     /gene="cysJ"
                     /locus_tag="OO369_002046"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR5982324.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8715..10427
                     /gene="cysI"
                     /locus_tag="OO369_002047"
     CDS             8715..10427
                     /gene="cysI"
                     /locus_tag="OO369_002047"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR5982325.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10501..11235
                     /gene="cysH"
                     /locus_tag="OO369_002048"
     CDS             10501..11235
                     /gene="cysH"
                     /locus_tag="OO369_002048"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR5982326.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11499..11651
                     /locus_tag="OO369_002049"
     CDS             11499..11651
                     /locus_tag="OO369_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR5982327.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11845..12936
                     /locus_tag="OO369_002050"
                     /pseudo
     CDS             11845..12936
                     /locus_tag="OO369_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(13082..14119)
                     /gene="iap"
                     /locus_tag="OO369_002051"
     CDS             complement(13082..14119)
                     /gene="iap"
                     /locus_tag="OO369_002051"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR5982328.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14371..15279
                     /gene="cysD"
                     /locus_tag="OO369_002052"
     CDS             14371..15279
                     /gene="cysD"
                     /locus_tag="OO369_002052"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR5982329.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15281..16708
                     /gene="cysN"
                     /locus_tag="OO369_002053"
     CDS             15281..16708
                     /gene="cysN"
                     /locus_tag="OO369_002053"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR5982330.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16708..17313
                     /gene="cysC"
                     /locus_tag="OO369_002054"
     CDS             16708..17313
                     /gene="cysC"
                     /locus_tag="OO369_002054"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR5982331.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17363..17686
                     /locus_tag="OO369_002055"
     CDS             17363..17686
                     /locus_tag="OO369_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR5982332.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17880..18191
                     /gene="ftsB"
                     /locus_tag="OO369_002056"
     CDS             17880..18191
                     /gene="ftsB"
                     /locus_tag="OO369_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR5982333.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18210..18920
                     /gene="ispD"
                     /locus_tag="OO369_002057"
     CDS             18210..18920
                     /gene="ispD"
                     /locus_tag="OO369_002057"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR5982334.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18920..19399
                     /gene="ispF"
                     /locus_tag="OO369_002058"
     CDS             18920..19399
                     /gene="ispF"
                     /locus_tag="OO369_002058"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR5982335.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19396..20445
                     /gene="truD"
                     /locus_tag="OO369_002059"
     CDS             19396..20445
                     /gene="truD"
                     /locus_tag="OO369_002059"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR5982336.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20426..21187
                     /gene="surE"
                     /locus_tag="OO369_002060"
     CDS             20426..21187
                     /gene="surE"
                     /locus_tag="OO369_002060"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR5982337.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21181..21807
                     /gene="pcm"
                     /locus_tag="OO369_002061"
     CDS             21181..21807
                     /gene="pcm"
                     /locus_tag="OO369_002061"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR5982338.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21947..23086
                     /gene="nlpD"
                     /locus_tag="OO369_002062"
     CDS             21947..23086
                     /gene="nlpD"
                     /locus_tag="OO369_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR5982339.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23149..24141
                     /gene="rpoS"
                     /locus_tag="OO369_002063"
     CDS             23149..24141
                     /gene="rpoS"
                     /locus_tag="OO369_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR5982340.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24261..24668)
                     /locus_tag="OO369_002064"
     CDS             complement(24261..24668)
                     /locus_tag="OO369_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR5982341.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24815..25408
                     /locus_tag="OO369_002065"
     CDS             24815..25408
                     /locus_tag="OO369_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR5982342.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25408..26355
                     /locus_tag="OO369_002066"
                     /pseudo
     CDS             25408..26355
                     /locus_tag="OO369_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26628..27191)
                     /locus_tag="OO369_002067"
     CDS             complement(26628..27191)
                     /locus_tag="OO369_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5982343.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27356..27607
                     /locus_tag="OO369_002068"
     CDS             27356..27607
                     /locus_tag="OO369_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5982344.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27609..29705
                     /locus_tag="OO369_002069"
     CDS             27609..29705
                     /locus_tag="OO369_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5982345.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         7295 a         7383 c         8062 g         7057 t
ORIGIN      
        1 tcgtcacatc atcatcggca acgaaggtgc attgtgctat atcactgaag taacagtgaa
       61 aatctttaaa tacaccccgg aaaacaacct cttctacggc tatatcctgg aagacatgaa
      121 aaccggcttc aacatcctgc gtgaaatcat ggtggaaggg tatcgtccgt cgatcgctcg
      181 tttgtatgac gctgaagatg gcacccaaca cttcacccat tttgccgacg gaaaatgtgt
      241 gctgatcttt atggctgaag gtaaccctcg cattgcgaag gcgacgggcg aagggattgc
      301 ggaaatcgtt gcccgctacc cgcagtgcca gcgcgtggac agcaaactga ttgaaacctg
      361 gtttaacaac ctgaactggg gaccggataa agtggctgcc gaacgtgtgc agatcctcaa
      421 aaccggcaac atgggcttta ccaccgaagt gtccggctgc tggagctgca tccacgaaat
      481 ctacgaaagc gttattaacc gtattcgtac tgagttcccg cacgccgacg acatcaccat
      541 gctgggcggt cactcctctc atagctatca gaacggcacc aacatgtact tcgtctatga
      601 ctacaacgtt gttgactgta agccggaaga ggaaatcgac aagtaccaca atccgctcaa
      661 caaaatcatc tgcgaagaaa ccattcgcct cggcggttcg atggtgcacc accatggcat
      721 tggtaaacat cgcgttcact ggagcaagct ggaacacggc agcgcgtggg cgttgctgga
      781 agggctgaaa aagcagttcg atcctaacgg cattatgaat acgggtacta tctatccgat
      841 tgaaaaataa tgtatcaggc agcgttccgc gatgacgggg cgttgccttt tcggcttctc
      901 aggcgagaag ccgttcttat taccggacaa tgaaggggta aagatgaaca cttcaccggt
      961 gcgaatggat gatttaccgc ttaaccgttt tcactgtcga attgctacgc tcactttcgg
     1021 cgcacacctg accgacggtt atgttctagg cgtcattggt tacgccatta ttcaacttac
     1081 gcccgccatg caactgacgc cgtttatggc gggaatgatc ggcggctcgg cgctccttgg
     1141 tttgtttctt ggcagcctgg ttcttgggtg gatctccgac catattggtc ggcaaaaaat
     1201 cttcaccttc agctttttgc tgattacgct cgcttcgttc ttgcaatttt ttgccaccac
     1261 gccagagcat cttattgggc tgcgcatttt gatcggcatt ggtctgggag gcgattactc
     1321 agtaggtcac accttgctgg ctgaattttc cccgcgccgc catcgcggta ttttgctggg
     1381 cgcattcagc gtggtgtgga ccgtaggcta tgtgttggca agtattgccg gacatcactt
     1441 tatttccgaa aacccggagg cctggcgctg gttgctggca tcggcagctc tgcccgcgtt
     1501 gttgattacg ttattacgct ggggaacgcc agaatcgcca cgttggctac tgtgccaggg
     1561 gcgttttgca gaagctcacg ctatcgtgca tcgctatttt ggtccccatg ttttactggg
     1621 cgatgaagtg gtaacggcga cccataaaca catcaaaacc ttgttctctt cgcgttactg
     1681 gcggcgcacg gcgtttaaca gcgtcttctt tgtctgcctc gtaatcccat ggtttgtgat
     1741 ttatacctgg ctgccaacta tcgcccagac tattggtctg gaagatgcgc tgactgccag
     1801 cctgatgctt aatgcgttgt taattgtggg cgcgctgctg ggattagttc tgacgcacct
     1861 gctggcacat cgcaaatttt tgctgggaag ttttttgctg ctggcggcaa cgctggtagt
     1921 aatggcctgt ttgccttccg gcagttcatt aacgctgctg ctttttgttc tcttcagcac
     1981 caccatttcg gcagtcagta atctggtggg cattttgcct gcggaaagtt ttcctactga
     2041 cattcgctcg ctgggagtcg gttttgccac cgccatgagt cgacttggcg cggcggtaag
     2101 tactggcctg ctgccgtggg tgctggcgca gtggggaatg caagtcacct tattgctcct
     2161 agcgacagtg ttgttggttg gttttgttgt gacctggcta tgggcaccag aaactaaagc
     2221 cctcccgctg gtggcggcgg gaaatgtagg aggtgcgaat gaacattctg ttagcgttta
     2281 aagccgaacc ggatgccgga atgctggcgg aaaaagagtg gcaggcggcg gctcagggta
     2341 atagcggacc ggatgtttcg ctactgcgaa gtttactcgg tgctgatgaa caggccgctg
     2401 ccgcgctgct gttagcgcag agaaaaaacg gtacgccgat gtctttaacc gcgttgagca
     2461 tgggggatga acgggcgttg cactggctgc gctatctcat ggctcttggg tttgaggaag
     2521 ctgtattgct ggagacggcg gcagatctgc gctttgcgcc ggaatttgtc gctcgccata
     2581 ttgccgaatg gcagcgtcag aatccgctgg atctgataat taccggcagc caaagcagcg
     2641 aagggcagaa tgggcaaacg ccatttttgc tggcggaaat gctgggctgg ccctgcttca
     2701 cccaggtgga gcgtttcact ctcgacgcgc tgtttatcac cctcgaacag cgtactgaac
     2761 atgggttgcg ctgttgccgg gttcgcttgc ctgcggtgat tgccgtgcgt cagtgcggtg
     2821 aagtggcctt gccagtaccg ggaatgcgcc agcgaatggc tgcggggaaa gcagaaatta
     2881 tccgtaaaac tgttgctgcc gaaatgccag cgatgcagtg tctgcaactg gcaagagcag
     2941 agcaacgacg cggagcgacg ctgattgatg ggcaaacggt agcagaaaaa gctcaaaagc
     3001 tatggcagga ctatttacgc caaaggatgc agccatgaat atcgcaattg tgacaataaa
     3061 tcaggagaat gccgcaatag cgagctggct ggcggcgcag gatttcagcg gttgtacgct
     3121 ggcgcactgg caaatagaac ctcagcccgt agtggcggaa caggtgctgg atgcgatggt
     3181 ggaacagtgg cagcgaacgc ctgcggacgt tgttctcttt ccgccgggaa cgtttggcga
     3241 tgaactgtcg acccggctgg cctggcgttt acatggtgca agcatctgtc aggtgacttc
     3301 gttagatatt ccaaccgtaa gcatgcgcaa atcccactgg ggaaatgcgt taacggcgac
     3361 gctgcaaaca gaaaaacgcc cgttatgcct ttccctggcg cgtcaggcgg gagcagcgaa
     3421 aaacgccacg ttgccctctg gaatgcagca actgaacatt gtcccaggtg cgcttcccga
     3481 ctggctggtt agcacagagg atctgaaaaa tgttagccgc gacccattag ctgaggcaag
     3541 acgggtgctg gtggtgggac agggcggaga ggcagataac caggaaatcg ttatgctggc
     3601 agaaaagctg ggagccgaag tgggctacag tcgggcgcga gtgatgaacg gtggcgttga
     3661 tgctgaaaaa gtgattggta tttccggaca tctgctggca cctgaggtgt gcattgttgt
     3721 tggcgcttcc ggcgcagcag cgttaatggc gggcgtacgc aatagcaagt ttatggtggc
     3781 gattaatcat gacgccagcg cagcggtatt ttcacaggca gatattggcg tggttgatga
     3841 ctggaaagtg gtgctggagg cactggtgac aaacattcac gcagaatacc aataacgtgc
     3901 tgccggatga tcctgcctac ggttctggac tggggacctg taggcctgat aagacgcgtt
     3961 aagcgtcgca tcaggcgctg aatgtcggat gtggcgtgcg gccaacaaat caaaacaaat
     4021 tcaattggtt acgattatcc cgtcaaagca attttttcgc taacgtccag actccggtat
     4081 tcgtggtgga aagcgccacg acacccgcgt taatcgcatt acgcgcatct tcttcatcgc
     4141 ataccagccc gccagcaatc agcggttggc ggattttttc cgtcacccag cccagcactt
     4201 tgggcatgca gcctggcagg atctcaatac aatccggatt cgattgcgca acttacttat
     4261 caatgttgtg aaacgaaatc gaatcaacaa taaacaggtg atgaatacag aaaaaaactt
     4321 ccgctcttgc cgcttttagc atcgatgctt tggtgctgat aattccatcc gcctcggtca
     4381 ccagtttcaa aaactgaatc accacttctt tatttgacgc gccttccagc aaatcaacat
     4441 gaataaaagc atatttcccg gcgttcttaa tctttttaac gatattactg atggtgcaga
     4501 tattgccgta caacacggaa ataaactggc attcagaatc gatggccagt tgcaggctgg
     4561 cattatcttt aacggcagca atcaccggat tctggcggag caggtgtaag aggggcataa
     4621 attatcctta ttatttatcc aaagcggtag gtgatgccga atccggaagg cggatagcgc
     4681 cactgttgta gtgtggattc gtcgcacagc aaacggcagg tgccacactc caggcagccg
     4741 cgatagtcaa tacgtaagtc accttcctgt gtgagagaaa aaagacctac cggacaggcg
     4801 ttaatcaacc gttgtaccat ctgacgctca acggagtcag ccggaacaat gtgcggcgca
     4861 tcagcaacgc gccagagatt acgggctaca gacatcgcag actcctgata acatcgcccg
     4921 ccagatgcca caggccatga cgacgtaaat gatgccagaa taactggcgc agcggtggaa
     4981 caggtttatc accctgatcc cataaatcgc gggaaatatc ctgcattaac gcaggccacg
     5041 cccgatacct gccagggcgt tgcaaaagcg ccggaacatg ctgataacgt tgtagaacat
     5101 cccatagcag gctgcgctct acgttgtgat gataaagcgc aaacagattt tgcggctcgc
     5161 ggtgctggca ggcgcttatt agcgtttgtg ccgccgcctg tgcgccagtc agcgccatat
     5221 ccatgccgcg cacggaaatt ccggtattga cgcaactgcg caacgcatcg cccaccagca
     5281 gccagccgtt accggcgtat tgcaccggca tactgtgcaa gccaccttct ggcaccagat
     5341 gcgcaccata ctccagtgat tccgtgtttt tgataagcgt gcgcactgcc ggatgcgctt
     5401 taaagcgagt cagcagctcg cttgccggaa cacgactttg cgtaagggaa gagagcgggc
     5461 aaacaatccc taacgagagc gtttgttgat tagtataaag aaatgcgccg ccgggtaagt
     5521 catcacagat tccgccgctg aacagcaacg ctgcgccttc gttattctcc agatgaaaac
     5581 gttcttcaat agcggatgtt tccagcgaca gcacttcttt gatccccaac gccatcgctt
     5641 cgccagcagg acgagtcact aacccgtgac gttcagccag gacgctgttg gcaccttctg
     5701 ccagcaccac ataacgggcg cggagaatat cgtcaccaca aataacgcca cagactctgc
     5761 cgttttcttc atacagtgca tccaccgtcg ctccggggat gcattcgaca ccttcttttt
     5821 cggcttcggc aaccagccac ggatcgaacc gtgcacgtaa tacattccag gattcaccgc
     5881 cgggctgtaa gctggaaaac gtcgttgcgc catccggcgt taacagggaa aggctttcgt
     5941 gagtgatgcg tcgttcaaga ggcgcggtca gatgaaattg cgggaggagt tccgcgagtg
     6001 catgggtata taaccgcctg ccggaaagat ttttgctgcc ggggatttca gcgcgttcga
     6061 gtaacaaaac ggataaatcc gctcgcgcgc agcgtaacgc gcaagcggtg cctgcaatac
     6121 cggcaccaat aataataatg tcgcagtcgt cttccatgtc atgccccaga tatccagaac
     6181 acaacaccct aacatagcgt tactaaaggg aaattgaccg ccgacacact cttatcattc
     6241 gccgcgatag atacaccctg cggtgcaggt ttcttttacc atcaccgcac ttaacagcgg
     6301 cacaacgggt ttaacctgat cccaaatcca ttttgctaaa acttcgctgg ttggattttc
     6361 cagacctgga atatcattga gataatggtg atcgaggcgc tcataagttg gcttaaacgc
     6421 cgctttcagt tcagcgaaat cgataatcca gcccgtatgc ggatcgactt ccccggtaat
     6481 ttccagtcgc accataaagg aatgcccgtg caggcgacca catttatgcc cttccgggac
     6541 atgtggtaag cggtgagcgg cttcgaaggt gaaatcttta aataacgtgg tggacatcat
     6601 aatttctcta caggacgata aaaccgccgt agagtaccgg aaaggctgtt ttttcgcatt
     6661 atctaaaacg actttgttgc gcaaaatcgc tgatttatct taatgattgg ctaaattcat
     6721 ttgtttttca ttaggttggt taatctattt tgttgttaaa gactattgct aaaacaggtt
     6781 agtcgatttg gttattagtt atcgctatcc cgtctttaat ccacaccgtt caccccgtta
     6841 accttacctt ctcttctgtt ttatgggcgc tgacagggcg cagaaacagc tttgcttact
     6901 ggaacataac gacgcatgac gacacaggtc ccaccttccg cgttgcttcc gttgaacccg
     6961 gagcaactgg cacgccttca ggcggccacg accgatttaa cgcctaccca gcttgcctgg
     7021 gtttctggct atttctgggg cgtgctcaac cagcagcctg ttgcgcttgc agtgacgcca
     7081 gcgcccgccg cagaaatgcc gggtataacg attatctctg cttcgcaaac cggcaatgcg
     7141 cgccgggttg ctgaagcatt acgcgatgat ttattagcag caaaactgaa cgttaagctg
     7201 gtgaacgcgg gcgactataa attcaaacaa atcgccagcg aaaaactgct catcgtagtg
     7261 acgtcaacgc aaggggaagg ggaaccgccg gaagaagccg tcgcgctgca taagttcctg
     7321 ttctccaaaa aagcgccaaa gctggaaaac accgcgtttg ccgtgtttag cctcggcgat
     7381 agctcttatg aatttttctg ccagtccggg aaagattttg acagcaagct ggcggaactg
     7441 gggggtgaac gcctgctcga ccgtgtcgat tctgatgttg aataccaggc tgctgccagc
     7501 gagtggcgcg cccgcgtggt tgatgcgctt aaatcgcgtg cgcctgtcgc ggcaccttcg
     7561 caatccgtcg ctactggcgc ggtaaatgaa atccacacca gcccgtacag caaagacgcg
     7621 ccgctggtgg ctagcctctc tgttaaccag aaaattactg ggcgtaactc tgaaaaagac
     7681 gttcgccata tcgaaattga cttaggcgac tcgggcctgc gttaccagcc gggtgacgcg
     7741 ctgggcgtct ggtatcagaa cgatccggca ctggtgaaag aacttgtcga actgctgtgg
     7801 ctgacaggcg atgaacctgt cactgtcgag ggcaaaacgt tgcctctgaa cgaagcgcta
     7861 cagtggcact tcgaactgac cgtcaacacc gccaacattg ttgagaatta cgccacgctt
     7921 acccgcagtg aaacactgct gccgctggtg ggcgataaag cgaagttaca gcattacgcc
     7981 gcgacgacgc tgattgttga catggtgcgt ttctccccgg cacagcttga tgccgaagcg
     8041 ctaattaatc tgctgcgccc gctgaccccg cgtctctatt ccattgcctc ctcgcaggcg
     8101 gaagtcgaga acgaagtaca cgtcaccgtt ggtgtggtgc gttacgacgt ggaaggccgc
     8161 gcccgtgccg gtggtgcctc cagcttcctc gctgaccgcg tggaagaaga gggcgaagtc
     8221 cgcgtattta tcgaacataa cgataacttc cgcctgcccg ctaacctgga aaccccggtg
     8281 attatgattg gcccaggcac cggtattgcg ccgttccgcg cctttatgca gcaacgcgcc
     8341 gctgacgaag cgccaggtaa aaactggctg ttctttggca atccgcattt tacggaagac
     8401 ttcctgtatc aggtggagtg gcagcgctac gtcaaagagg gtgtgctgac gcggatcgat
     8461 ctcgcctggt cgcgcgacca aaaagaaaaa gtttacgtac aagataaact gcgcgaacag
     8521 ggcgcggagc tgtggcgctg gatcaatgac ggtgcccaca tttatgtctg cggcgacgct
     8581 aatcgcatgg cgaaagacgt tgagcaggca cttctggaag tgattgccga atttggtggc
     8641 atggacaccg aagcggcgga tgaattttta agtgagctgc gcgtagagcg ccgttatcag
     8701 cgagatgtct actaatgagc gaaaaacatc cagggccttt agtggtcgaa ggaaaactga
     8761 cagacgccga gcgcatgaag cttgaaagca actacctgcg cggcaccatt gcggaagatt
     8821 taaacgacgg tctgaccggc ggctttaagg gcgataactt cctgctgatc cgcttccacg
     8881 gcatgtatca gcaggatgac cgcgacatcc gcgccgaacg tgctgaacag aagctggagc
     8941 cgcgccacgc gatgctgttg cgctgccgtc tgccgggtgg agttatcacc accaaacagt
     9001 ggcaggcgat cgataaattt gccggtgaaa acaccatcta tggcagcatt cgcctgacca
     9061 accgccagac gtttcagttc cacggcattc tgaaaaagaa cgtcaaaccg gtgcaccaga
     9121 tgctgcactc ggtcggtctt gatgcgctgg cgactgctaa cgacatgaac cgtaacgtac
     9181 tctgcacctc gaacccttac gagtcgcagc tgcacgcaga agcgtacgag tgggcgaaga
     9241 agatttctga acatctgctg ccgcgtaccc gcgcgtatgc ggagatctgg ctcgatcagg
     9301 aaaaagtcgc caccaccgat gaagaaccga tccttggtca gacttacctg ccgcgtaagt
     9361 tcaaaaccac ggtagtgatc ccgccgcaga acgatattga tctgcacgcc aacgacatga
     9421 acttcgtggc gatcgcggaa aacggcaagc tggtgggctt caacctgctg gtgggcggtg
     9481 ggctttctat cgaacacggt aacaagaaaa cctacgcccg cacggcgagt gagtttggct
     9541 atctgccgct agagcatacg ctagcggtgg cggaagccgt cgtgacaact cagcgtgact
     9601 ggggtaaccg aaccgatcgt aaaaatgcca aaaccaaata cacactggag cgcgtggggg
     9661 ttgagacgtt taaagcggaa gtggagcgtc gcgcggggat caaatttgaa ccgatccgcc
     9721 cgtatgaatt tacaggacgc ggcgatcgta ttggctgggt taagggcatt gatgataact
     9781 ggcacctgac gctgtttatc gaaaatggtc gcatccttga ttatccgggg cgtccgctga
     9841 aaactggcct gctggagatc gcgaagatcc acaaaggtga tttccgcatt acggcgaacc
     9901 agaatctgat catcgccggt gtgccggaaa gcgagaaagc gaagatcgag aaaattgcca
     9961 aagagagcgg gttaatgaat gccgtcacgc cgcagcgtga aaactcgatg gcctgcgtgt
    10021 cattcccgac ttgcccgctg gcgatggcgg aagcggagcg tttcctgccg tcttttatcg
    10081 acaacatcga taatttaatg gtgaaacatg gtgtcagcga tgagcatatc gtgatgcgtg
    10141 taacaggctg cccgaacggt tgtggtcgcg cgatgctggc ggaagtgggc ctggtgggta
    10201 aagcaccggg tcgctacaac ctgcatcttg gcggcaaccg cattgggaca cgtatcccac
    10261 ggatgtataa agaaaacatc accgagccgg aaatcctggc gtcgcttgat gaactgatag
    10321 ggcgctgggc gaaagagcgc gaagcgggtg aaggcttcgg cgactttacg gtgcgtgcgg
    10381 gcatcattcg cccggtgctc gatccggcgc gcgatttgtg ggattaacca tcagcccggt
    10441 cttgtaggcc tgataagacg cgtgagcgtc gcatcaggca aggcaaacag tgaggaatct
    10501 atgtccaaac tcgatctaaa cgccctgaac gaactgccga aggtagatcg cattctggcg
    10561 ctggcagaaa ctaacgccga actggaaaaa ctggacgctg aaggccgcgt tgcctgggcg
    10621 ctggataatc tgcccggtga atatgtactt tcttccagct ttggcattca ggcggcggtg
    10681 agcctgcatc tggtgaatca gattcacccg gatattccgg tgatcctcac cgacaccggc
    10741 tatctgttcc cggaaaccta ccgctttatt gacgagttaa cggacaaact caagctcaac
    10801 ctgaaagtgt accgcgctac cgaaagcgcc gcctggcagg aagcacgcta cggcaaactg
    10861 tgggaacagg gcgttgaagg cattgaaaag tacaatgaca tcaacaaagt cgaaccgatg
    10921 aaccgggcgc tgaaagaact gaatgcgcaa acgtggtttg ctggcctgcg gcgtgaacaa
    10981 tccggcagcc gtgccaattt accggtgctg gcaattcagc gtggcgtatt taaagtgctg
    11041 ccgattatcg actgggataa ccgaactatt tatcagtacc tgcaaaaaca tggcctgaaa
    11101 tatcacccat tatgggacga aggatattta tcggtgggcg atacccatac aacccgtaaa
    11161 tgggaacccg gcatggcgga agaagaaacg cgtttctttg gcttaaaaag ggaatgtgga
    11221 ttacacgaag ggtaaatatt gccggataaa ttaaacgcct ctgtcagaaa tgatgggggc
    11281 gtttttattt tgcgagcagc attccggcat ttaaaggtga acatcgcagg gaaaattaac
    11341 aggtatagaa tggcgttggg tgcttgaggc tgtctggcgt taagcattcg cgaggttcca
    11401 gatggacaaa agccccaggc gatatttcta tcaacctgag gctcgcgttc gaacgtagca
    11461 attcggatgt tagtctcttc tttgcaggga ggcaagacat gctgacaaaa tatgcccttg
    11521 tggcaatcat cgtactgtgt tgtacagtac tgggattcac gttgatggta ggtgactcgt
    11581 tatgtgagtt gagtatcaga gaacgtggta tggagtttaa ggcagttctc gcttacgaat
    11641 cgaagaagta gccaccacgc gggggtaaaa ccccgcgtac tgaattgtga tggtttggtc
    11701 tcctgcaccc tttttttaaa tttatatttt ttatgtgaat atatagtctc tatttattat
    11761 tatgaatttc aaatgaaaat cattaattta ttcataatgt aaatgtttta ttggctgata
    11821 tatatcatag atagttttta aaatatgcgt aaatatcctt taagtttact gaaggataaa
    11881 aatattgtga ctttctttga tttctgggga aaaaaccgac gtggcgagaa agagggtatc
    11941 gacgactatc accttctttg ctggcatttg ctggatgtgg ccgcaatggg ctatttaatg
    12001 gttaaaagaa attgcttcgg gctggctgat tattttcgtc aattagggat ttctgacaag
    12061 gaacaggcag ctcgattttt cgcctggttg ctgtgctggc acgatattgg aaaatttgcc
    12121 cgctcttttc aacaacttta cctgccccct gaattcaaga ttcaggaagg tgccagaaaa
    12181 aattacgaaa aaatctctca ttcaacgctg ggctactggt tgtggaatca ttatttaagt
    12241 gaatgtcagg agttacttcc ttcatcttcg ctctctccgc gcaaacttag gcgagtgata
    12301 gaaatgtgga tgcccgtgac gacagggcat catggtcggc cccctgatcg tatggatgaa
    12361 ttggataatt ttctgcctga agacaaagct gcagcacgag attttctcct tgaaatcaag
    12421 ccactgtttc cgcgaataga gatccccaca ttctgggatg atgacgaggg cgttgaactt
    12481 ataaaacaac tttcctggta tatctctgca accgtcgtac ttgcagactg gacagggtcg
    12541 tcaacgcgat tttttccacg cgtcgcacag gcaatggata ttgaagatta ctggcaaaag
    12601 gctttagttc aggctcaaaa cgcattaacc gtctttccgc cacaagcaga aaccgcgcct
    12661 ttcaccggaa ttaatacgct gtttcctttt attgagaata cgacaccgtt acagcaaaag
    12721 gtgctggatc tggatatcaa tcagccagga ccacagttgt ttattctgga agatgtgacc
    12781 ggcgcaggta aaacagaagc ggcgcttatc ctggcgcaca ggttgatggc tgcgggtaac
    12841 gctcaggggc tgttttttgg cctgccaaca atggcaacgg ccaatgccat gtacgatcga
    12901 ctggtcaaaa cctggcttgc tttctattcg ccagaggtgt tccccgctcc agcggagata
    12961 aaccggcgca atacggtgcg gttttcccgg aagtaaaccg gacgcactgg atgcgatgat
    13021 gggtatcact tagaattccc cgcccctgcg gtagaactcc cagctcccat tttcaaaccc
    13081 atcaagacgc cttcgccagc tccttcacca acggtagcat tatccgcata acgtcacggc
    13141 agcgacgttc tattcttcca ggaagtgcct tatcaatatg ttgttgatta tccagtctta
    13201 cgtcatgcca gctatttccc gccgggaagg caggtgtttt tgcgcgttgc tgataaccat
    13261 ccttatttcc aagattccag ttagtcgctt ccaccgaaag tacagcaatg cccgctttgt
    13321 cgaatatttc tgcgtcatta caacccccag tgcctttcgg ataattttta ttcaaacccg
    13381 gattggtcgt tgcggctatt ccgtgactgc gtgcaattgc cagcgccctg tcgcgcgtta
    13441 atttccttac tgcttcaggg gtttttacac cgctgttgaa atacaattta tcgccaacaa
    13501 ttaagttatc gagattaatc accagcagcg tatttttctt ttcggtgtca ctcatccgct
    13561 tgagtaaatt ctcagcgcct aattttcctt cctcttcgcc gctggtcgcc acaaatcgaa
    13621 taccatactc ggtaggcgta tttttcaggc gttctgccag ttccagcatg acacctaaac
    13681 ctgcggcgtt atcatctatt ccttgtaacg tcagtccgcc gagattggca tctgcgtcag
    13741 catcgctcag cggggcgtaa gtatccagat gcgccataat gatgatctgc tgcggcgctt
    13801 tgccttcatg agcggcaatc accgtacttc ctgtcacgtt atgccagttc ttgcgattat
    13861 cgcgggcggt ataaatatac cgactattaa atgtccgaat atcactgcga taacccattt
    13921 gctgaaactg ttggcgaata taatcggcag ataacatttc tgctggagtt ccggtcatgc
    13981 gtcccggaaa gaaagtagca atatgtcgtg cctgggtatt agcaaaatcg ccaggtttag
    14041 gtgacgaggc gtgtacgggg agaataaagc atacgccgag cgccagggca gcggtacggt
    14101 ggcgcaatgc ggaaaacata gtgagtcctt aaataccatg caaatttttt taccgccata
    14161 gtatgaaact gccgctgcgc taaaacaatt tcaaatcttc ctaaacgccc gaaatccggt
    14221 gccttaagca ctttttgata ttagctttgc caaatcgtta ttccgttaag gaactactca
    14281 ttctaattgg taatttcatt cgttctctta cgctccctat agtcgaaaca tctgatggca
    14341 agaaaatagc ggtattgcaa aggaacggtt atggctcaaa aacgacttac tcacctgcgg
    14401 caactggagg cggaaagcat ccacattatt cgcgaggtgg cggcagaatt ctcaaatccg
    14461 gtgatgctct actctatcgg taaagattcc agcgtcatgc tgcatctggc gcgcaaggcg
    14521 ttttatccag gtacgctgcc tttcccgttg ctgcatgtcg ataccggctg gaaattccgc
    14581 gagatgtatg agttccgcga tcgtacggcg aaagcctacg gctgcgagct gctggtgcat
    14641 aaaaacccgg aaggcgtggc gatggggatt aatccattcg tgcacggcag cgcgaaacat
    14701 accgatatta tgaaaactga aggcctgaaa caggcgctga acaaatacgg ttttgatgcc
    14761 gccttcggtg gtgcgcgccg tgacgaagag aaatcccgcg ctaaagagcg aatctactct
    14821 ttccgtgacc gcttccatcg ctgggatccg aaaaatcagc gcccggagct gtggcacaac
    14881 tacaacgggc aaattaacaa aggcgaaagc atccgcgtct tcccgctctc taactggacc
    14941 gagcaggata tctggcaata catctggctg gaaaatatcg acattgtgcc gctgtatctc
    15001 gctgcggaac gtccggttct ggaacgcgac ggtatgttga tgatgattga tgacaaccgt
    15061 atcgacctgc aaccgggcga agtgatcaaa aaacggatgg tgcgtttccg tacgctgggc
    15121 tgctggccgc tgaccggtgc ggtggagtca aatgcacaaa cactgccgga aatcatcgaa
    15181 gagatgctgg tttccaccac cagtgaacgt cagggtcgcg tgattgaccg cgaccaggcg
    15241 ggatccatgg agctgaaaaa acgtcagggg tatttttaag atgaacaccg tacttgcaca
    15301 acaaatcgcc aatgaaggcg gcgtcgaagc ctggatgatt gcgcaacaac ataaaagcct
    15361 gctgcgtttt ctgacctgtg gtagcgtcga tgacggcaaa agtaccctga ttggtcgtct
    15421 gctgcacgat acccgccaaa tctacgaaga tcagctctca tcgctgcata acgacagtaa
    15481 gcgtcacggc acccagggcg aaaagctgga tctggctctg ctggtggacg gcctgcaagc
    15541 tgagcgcgaa cagggcatca ctattgatgt ggcctaccgc tatttctcta ccgagaagcg
    15601 taaatttatt atcgccgaca ccccagggca cgagcagtac actcgcaata tggcgactgg
    15661 cgcatcgaca tgtgaactgg cgatcttact gattgatgcc cgtaaaggcg tgctcgatca
    15721 aacccgtcgt cacagtttta tctccacact gttggggatc aaacatctgg tcgtggcgat
    15781 caacaaaatg gatctggtgg attacagtga agagacgttc acccgtattc gtgaagatta
    15841 tctgaccttt gccgggcagc tgccgggtaa tctggatatc cgctttgtgc cgctctccgc
    15901 actggaaggt gacaacgtgg cttcgcaaag tgaaagtatg ccgtggtaca gcggtctgac
    15961 actgctcgaa gtgctggaaa ccgtggagat ccagcgagtg gtggatgccc agccaatgcg
    16021 cttcccggtg cagtacgtta accgcccaaa tctcgatttt cgtggctacg ccggaatgct
    16081 ggcatccggt cgcgtggaag tcgggcaacg tgtaaaagtg ctgccctctg gtgtggaatc
    16141 aaacgtcgcg cggatcgtga cttttgatgg tgatcgcgaa gaagtctttg ccggagaagc
    16201 gatcaccctg gtgctgacgg atgagatcga tatcagccgt ggtgatctgc tgctggcggc
    16261 agacgaagcg ttaccggcgg tgcagagtgc gtcggtggat gtggtatgga tggcggaaca
    16321 gccgctttcc ccgggccaga gttatgacat caaaattgcc ggtaagaaga cgcgtgctcg
    16381 tgttgatggc attcgttatc aggttgatat taataacctt acccaacgcg aagttgaaaa
    16441 cctgccgctg aacggcatcg gcctggtgga tctcactttt gacgagccac tggtgttaga
    16501 tcgctatcag caaaatccgg ttaccggtgg gctgattttt atcgatcgcc tgagcaatgt
    16561 gaccgtgggt gccggtatgg tgcacgagcc agttagccag gcaacagctg cgtcatctga
    16621 attcagtgca ttcgaactgg aattgaatgc cctggttcgt cgccactttc cgcactgggg
    16681 cgcgcgcgat ttgctggggg ataaataatg gcgctgcatg acgaaaacgt cgtctggcat
    16741 agtcatccgg tcactgtgca acaacgcgaa ctacaccacg gtcatcgtgg tgtagtgctg
    16801 tggtttaccg gcctctccgg gtccggtaaa tcaacggtcg ccggggcgct ggaggaggcg
    16861 ttacataaac tcagcgtcag tacgtatctg ctggatggcg acaatgttcg ccacggatta
    16921 tgcagcgatc tcggttttag cgatgccgat cgtaaagaga atatccgtcg cgtcggtgaa
    16981 gtggcgaatt tgatggttga agccggactg gtggtgctga ccgcatttat ctcgccacac
    17041 cgcgccgaac gccagatggt tcgcgaacgc gtaggagaag ggcgctttat tgaagtgttt
    17101 gtcgatacgc cgctggcgat ttgcgaagcc cgcgatccaa aaggcttata taagaaagcg
    17161 cgtgccggtg aactgcgcaa ctttacggga atagattccg tttacgaagc gcctgaatcg
    17221 gcagaaattc atctcaatgg tgaacaatta gtaacaaatt tggtacagca attattagat
    17281 ctgttgagac agaacgatat tatcagatcc tgagacacca ccgggtttcc atgcccggtt
    17341 cgtcaaagta acaggatcaa atatgcgtaa tagccataac attacactaa caaataacga
    17401 cagccttact gaggatgaag aaactacatg gtcactgcct ggtgccgtgg tcggttttat
    17461 ctcctggtta tttgcgctgg cgatgccgat gttgatttat ggctctaaca cgctgttctt
    17521 ctttctctac acctggcctt tctttctggc actgatgcct gtcgcggtag tggtggggat
    17581 tgcgctgcat tcattgatgg acggaaagct acgctacagt attgttttca ccctggtgac
    17641 agtaggcatt atgtttggcg cactgtttat gtggctactg ggctaatttg tactttcttg
    17701 tctcctctgt tcataattca aaccgtaact aataatgaga ttatgttctg cacgccctgg
    17761 gtatacgtaa caatgggcaa atgtggtaca tttgcccgcg ttgtcgcggt atccccaaca
    17821 gaggatgtag agtcgtcttc ggatgcatgg gatgatgatg cagtttttca gggggcagga
    17881 tgggtaaact aacgctgctg ttgctggcta ttctggtctg gctacagtat tcgctgtggt
    17941 tcggtaagaa cggtatacat gactataccc gcgtcaatga tgatgtggcg gcactgcaag
    18001 ctacaaacgc gaaacttaaa gcgcgaaacg atcaactttt tgccgaaatt gacgatctca
    18061 atggcggcca ggaggcgctc gaagagcgtg cgcgtaatga actcagcatg accaggccgg
    18121 gcgaaacttt ttatcgtctg gtgcctgacg cgtcgaagcg cgcacagtct gcggggcaaa
    18181 acaatcgata aatcagcccg ggaattaaca tggcaaccac tcatttggat gtttgcgccg
    18241 tggttccggc ggccggattt ggccgtcgaa tgcaaacgga atgtcctaag caatatctct
    18301 caatcggtaa tcaaaccatt cttgaacact cggtgcatgc gctgctggcg catccccggg
    18361 tgacacgtgt cgtcattgcc ataagtcctg gcgatagccg ttttgcacaa cttcctctgg
    18421 cgaatcatcc gcaaatcacc gttgtagatg gcggtgatga gcgtgccgat tccgtgctgg
    18481 caggtctgaa agccgctggc gatgtgcagt gggtattggt gcatgacgcc gctcgtcctt
    18541 gtttgcatca ggatgacctt gcgcgattgt tggcgttgag cgaaaccagc cgcacgggag
    18601 ggatcctcgc cgcaccagtg cgcgatacta tgaaacgtgc cgaaccgggc aaaaatgcca
    18661 ttgctcatac cgttgatcgc aacggcttat ggcacgcgct gacgccgcaa tttttccctc
    18721 gtgagctgtt acatgactgt ctgacgcgcg ctctaaatga aggcgctacc attaccgacg
    18781 aagcctcggc gctggaatat tgcggatttc atccgcagtt ggtcgaaggc cgcgcggata
    18841 acattaaagt cacgcgcccg gaagatttgg cactggccga gttttacctc acccgaacca
    18901 tccatcagga gaatacataa tgcgaattgg acacggtttt gacgtacacg cctttggcgg
    18961 tgaaggccca attatcattg gtggcgtacg cattccttac gaaaaaggat tgctggcgca
    19021 ttctgatggc gacgtggcgc ttcatgcgtt gaccgatgca ttgcttggcg cggcggcgct
    19081 gggggatatc ggcaaactgt tcccggatac cgatccggca tttaaaggtg ccgacagccg
    19141 cgagctgcta cgcgaagcct ggcgtcgtat tcaggcgaag ggttataccc ttggcaacgt
    19201 cgatgtcact atcatcgctc aggcaccgaa gatgttgccg cacattccac aaatgcgcgt
    19261 gtttattgcc gaagatctcg gctgccatat ggatgatgtt aacgtgaaag ccactactac
    19321 ggaaaaactt ggatttaccg gacgtgggga agggattgcc tgtgaagcgg tggcgctact
    19381 cattaaggca acaaaatgat tgagtttgat aatctcactt acctccacgg taaaccgcaa
    19441 ggcaccgggc tgctgaaagc caatccggaa gactttgtgg tggtggaaga tttgggcttt
    19501 gagcctgatg gtgaaggtga gcatattctg gttagaatcc tcaaaaacgg ctgcaatacc
    19561 cgttttgtgg cggatgcact ggcgaaattc ctgaaaattc attcccgtga agtcagcttc
    19621 gctgggcaaa aagacaaaca tgctgtaacg gaacagtggt tatgtgctcg cgtgccgggc
    19681 aaggaaatgc ccgatctgag cgcctttcaa ctggaaggct gccaggtgct ggagtatgcg
    19741 cggcacaagc gcaagctgcg tttaggggcg ctgaaaggta acgcctttac cctggttctg
    19801 cgcgaagtga gcaatcgcga tgacgttgaa caacgtctga tcgatatttg cgtaaaaggt
    19861 gtaccgaact acttcggtgc ccaacgtttt gggattggcg gtagcaactt gcagggcgcg
    19921 ctgcgctggg cgcaaaccaa tactccggtg cgcgatcgca ataaacggag tttttggttg
    19981 tcggcagccc gcagtgcgtt gtttaatcag attgttgctg agcgcctcaa aaaagcagac
    20041 gttaatcaag ttgttgacgg cgatgcgcta caattagccg gacgtggtag ctggtttgta
    20101 gcaaccaccg aagaactggc ggaattacag cgtcgcgtca acgataaaga gctgatgata
    20161 accgccgcat tgccgggcag tggcgaatgg ggaactcagc gtgaagcgct ggcattcgaa
    20221 caagcagctg tcgccgcaga aactgaatta caagctttac tggtgcgcga aaaagttgaa
    20281 gccgcgcgca gagcgatgct gctgtatccg caacaattaa gctggaactg gtgggatgac
    20341 gtcaccgtag agatccgttt ctggcttccg gcgggtagtt ttgcaaccag cgttgtcagg
    20401 gaacttatca acacaacagg tgattatgcg catattgctg agtaattatg acggggtaca
    20461 tgcacccggt atacaaacgc tggcgaaagc cttgcgtgag tttgctgacg ttcaggtggt
    20521 cgcccccgat cgtaaccgca gcggcgcttc aaattctctg acactggaat cctccctgcg
    20581 cacgtttacc tttgaaaatg gtgatattgc tgtgcaaatg ggaaccccga ccgattgtgt
    20641 ctatcttggc gtgaatgctc tgatgcgtcc gcgcccggac attgttgtgt ccggaattaa
    20701 cgccgggccg aatctggggg atgatgttat ttattccggt acggtagccg ccgcgatgga
    20761 aggccgtcat ttaggttttc cggcgctggc cgtctcgctt gacgggcata aacattacga
    20821 cactgccgcg gcggtaacct gttcaatttt gcgcgcactg tgtaaagagc cgctgcgcac
    20881 cggacgtatt cttaatatta acgttcagga tttacccttg gatcaaatca aaggtattcg
    20941 cgtgacgcgc tgcggtacac gacatccggc agatcaggtg atcccgcagc aagatccgcg
    21001 cggcaatacg ctgtactgga ttggcccgcc gggcggtaaa tgtgatgccg gtccggggac
    21061 cgattttgct gcggtagatg agggctatgt ctccatcacg ccgctgcatg tggatttaac
    21121 tgcgcatagc gcgcaagatg tggtttcaga ctggttaaac agcgtgggag ttggcacgca
    21181 atggtaagca gacgcgtaca agcacttctg gatcaattac gtgcgcaagg tattcaggat
    21241 gagcaggtgc tgaatgcact tgccgccgtg ccgcgtgaaa aattcgttga tgaagcgttt
    21301 gaacaaaaag cctgggacaa tatcgccttg ccgataggtc aggggcagac aatttcgcag
    21361 ccgtatatgg tggcgcgaat gaccgaatta ctcgagctga cgccgcagtc gcgggtgctg
    21421 gaaattggca ctggttcggg atatcaaacg gcaatcctgg cgcatcttgt ccagcatgtt
    21481 tgctcggttg aacggattaa aggcttgcag tggcaggcac gtcgccgcct gaaaaatctt
    21541 gatttacata atgtttcaac ccgtcatggt gatggatggc aaggttggca ggcacgtgcg
    21601 ccgtttgacg ctatcattgt tacggcggca ccgccggaaa ttccaactgc gctaatgacg
    21661 cagctggacg aaggcgggat tctcgtctta cccgtagggg aggagcacca gtatttgaaa
    21721 cgggtgcgtc gtcggggagg cgaatttatt atcgataccg tggaggccgt gcgctttgtc
    21781 cctttagtga agggtgagct ggcttaaaac gtgaggaaat acctggattt ttcctggtta
    21841 ttttgccgta ggtcagcgta tcgtgaacat cttttccagt gttcagtagg gtgccttgca
    21901 cggtaattat gtcactggtt attaaccaat ttttcctggg ggataaatga gcgcgggaag
    21961 cccaaaattc accgttcgcc gcattgcggc tttgtcactg gtttcgctat ggctggcagg
    22021 ctgttctgac acttcaaatc caccggcacc ggtcagctcc gttaatggca atgcgcctgc
    22081 aaatactaat tctggtatgt tgattacgcc gccgccgaaa atggggacga cgtctacagc
    22141 gcagcaaccg caaattcagc cggtacagca gccacaaatt caggctactc aacaaccgca
    22201 aatccagcca gtgcagccag tagctcagca gccggtacag atggaaaacg gacgcatcgt
    22261 ctataaccgt cagtatggga acattccgaa aggcagttat agcggcagta cctataccgt
    22321 gaaaaaaggc gacacacttt tctatatcgc ctggattact ggcaacgatt tccgtgacct
    22381 tgctcagcgc aacaatattc aggcaccata cgcgctgaac gttggtcaga ccttgcaggt
    22441 gggtaatgct tccggtacgc caatcactgg cggaaatgcc attacccagg ccgacgcagc
    22501 agagcaagga gttgtgatca agcctgcaca aaattccacc gttgctgttg cgtcgcaacc
    22561 gacaattacg tattctgagt cttcgggtga acagagtgct aacaaaatgt tgccgaacaa
    22621 caagccaact gcgaccacgg tcacagcgcc tgtaacggta ccaacagcaa gcacaaccga
    22681 gccgactgtc agcagtacat caaccagtac gcctatctcc acctggcgct ggccgactga
    22741 gggcaaagtg atcgaaacct ttggcgcttc tgaggggggc aacaagggga ttgatatcgc
    22801 aggcagcaaa ggacaggcaa ttatcgcgac cgcagatggc cgcgttgttt atgctggtaa
    22861 cgcgctgcgc ggctacggta atctgattat catcaaacat aatgatgatt acctgagtgc
    22921 ctacgcccat aacgacacaa tgctggtccg ggaacaacaa gaagtgaagg cggggcaaaa
    22981 aatagcaacc atgggtagca ccggaaccag ttcaacacgc ttgcattttg aaattcgtta
    23041 caaggggaaa tccgtaaacc cgctgcgtta tttgccgcag cgataaatcg gcggaaccag
    23101 gcttttgctt gaatgttccg tcaagggatc acgggtagga gccaccttat gagtcagaat
    23161 acgctgaaag ttcatgattt aaatgaagat gcggaatttg atgagaacgg agttgaggtt
    23221 tttgacgaaa aggccttagt agaagaggaa cccagtgata acgatttggc cgaagaggaa
    23281 ctgttatcgc agggagccac acagcgtgtg ttggacgcga ctcagcttta ccttggtgag
    23341 attggttatt caccactgtt aacggccgaa gaagaagttt attttgcgcg tcgcgcactg
    23401 cgtggagatg tcgcctctcg ccgccggatg atcgagagta acttgcgtct ggtggtaaaa
    23461 attgcccgcc gttatggcaa tcgtggtctg gcgttgctgg accttatcga agagggcaac
    23521 ttggggctga tccgcgcggt agagaagttt gacccggaac gtggtttccg cttctcaaca
    23581 tacgcaacct ggtggattcg ccagacgatt gaacgggcga ttatgaacca aacccgtact
    23641 attcgtttgc cgattcacat cgtaaaggag ctgaacgttt acctgcgaac cgcacgtgag
    23701 ttgtcccata agctggacca tgaaccaagt gcggaagaga tcgcagagca actggataag
    23761 ccagttgatg acgtcagccg tatgcttcgt cttaacgagc gcattacctc ggtagacacc
    23821 ccgctgggtg gtgattccga aaaagcgttg ctggacatcc tggccgatga aaaagagaac
    23881 ggtccggaag ataccacgca agatgacgat atgaagcaga gcatcgtcaa atggctgttc
    23941 gagctgaacg ccaaacagcg tgaagtactg gcacgtcgat tcggtttgct ggggtatgaa
    24001 gcggcaacac tggaagatgt aggtcgtgaa attggcctca cccgtgaacg tgttcgccag
    24061 attcaggttg aaggcctgcg ccgtttgcgc gaaatcctgc aaacgcaggg gctgaatatc
    24121 gaagcgctgt tccgcaagta agtaagcatc tgtcagaaag gccagtctca agcgaggctg
    24181 gcctttttct tttgtttggg aagcttgtgt ttttgtcgcc tggataagac acgtcagcgt
    24241 cctacccggc aatcagcatt ctatgtgctc atcatcttgc gcaccaactg cataaacagc
    24301 tcctgctctt ctccactcaa acgccccaaa aattcctcat ccacgctgtc accaataggt
    24361 atcgccgcag caagtagctt ttctccttcg gcagtcagcc agacaaagcg tcgccgcttg
    24421 tcagcgacgt catgttcacg tctgactaag ccacgatttt ccattcttgc caacatttct
    24481 gctagcgttg ctttggtgct gactgctgct tctatcagtg ccacctgttc aataccaggt
    24541 ttatcagcaa tggcgcgcat caccgcgtac tgtggtttgg tcaggtcagg taactcgtgc
    24601 tgccagcgag ccgtgtgctg ctgaaaaagc tgtcgtaact gatggaacgc tttatttcgt
    24661 aacgccatgt aaactccggg ctaattctct gtcgctatca tagcggtttt ttagtgacga
    24721 agaagatgtt gttacttttc aatccttgcc gctgtggaaa tgaaggagta tgttaataat
    24781 aacgttcgta tacgaataat taagagaata agcaatgaaa ctgatcgtcg ggatgacagg
    24841 ggctaccggt gcgcctcttg gtgtggcatt actgcaagcg ctgcgtgaga tgccgaatgt
    24901 cgagactcat ctggtgatgt cgaagtgggc gaaaaccacc attgaactgg aaacgcctta
    24961 cagcgctcgc gatgttgctg ccctcgcaga cttcagccat aacccggcgg atcaggcggc
    25021 gatcatctca tccggttctt ttcgtaccga cggcatgatc gttattccgt gcagtatgaa
    25081 aacgctcgcc ggtatccgcg ctggttacgc cgatggcctg gtagggcgcg cggcggacgt
    25141 cgtgctcaaa gaaggccgca aactggtgct ggtgccgcgt gaaatgccgc ttagcaccat
    25201 ccatctcgaa aatatgctcg cactttcacg catgggcgtg gcgatggtgc cgccgatgcc
    25261 tgccttttat aaccatcccg aaacggtaga tgacattgtc caccatgtgg tagcccgcgt
    25321 gctggatcaa tttggcctcg aacatcccca cgccaggcgc tggcaaggat tgccgcaggc
    25381 ccggaatttt tctcaggaga atgaataatg gcatttgatg atttacgcag ctttttacag
    25441 gcgcttgatg accacggcca gttactgaaa atcagcgaag aagtgaacgc tgagccggat
    25501 ctggcagcag cagctaacgc caccgggcgt atcggcgacg gcgcgcccgc gctgtggttt
    25561 gataatattc gcggctttac cgatgcccgc gtggcgatga ataccatcgg ttcctggcag
    25621 aaccacgcga tttcccttgg cctgccacca aacaccccgg tcaaaaagca gattgatgag
    25681 tttatccgcc gctgggataa cttcccgatt gccccggagc gccgcgccaa tccagcctgg
    25741 gcgcagaaca ccgtcgatgg cgaagagatc aacctgttcg atattctgcc gctgtttcgc
    25801 ctgaatgatg gcgatggcgg tttctatctc gacaaagcgt gcgtggtttc ccgcgatccg
    25861 ctcgacccgg ataacttcgg caagcagaac gtcggcatct accgcatgga agtgaagggc
    25921 aagcgtaagc tcggcctgca accggtgccg atgcacgata tcgccctgca tctgcataaa
    25981 gcagaagagc gcggtgaaga tctgccgatt gcgatcacgc tcggtaacga tccgatcatc
    26041 acgctgatgg gggccacgcc gctgaaatac gatcagtctg agtatgaaat ggcaggcgcg
    26101 ctgcgtgaaa gcccgtaccc gatcgccacc gctccgctga ccggttttga tgtgccgtgg
    26161 ggttcagaag tgatcctcga aggggttatc gaaagccgta aacgcgaaat cgaagggccg
    26221 ttcggtgagt ttaccgggca ctactccggt gggcgcaaca tgaccgtggt gcgcatcgat
    26281 aaagtctctt atcgcagcaa accgattttc gaatcactct atctcggtat gccgtggacc
    26341 gaaatcgact acctatgact aggtaattta ttcaagataa ccgggaagag attaaaaaag
    26401 acgggaatag attggaatca acaagttagg aactcaaggt tttcaagata aagcgagtcc
    26461 aaaaatgtta aacggttcga tatcaataaa acattcaaga taactcacag gttcttacaa
    26521 tagcctttaa agaccgttca aaaacgattc aggtgtttat tatagcgctg atgggtgcaa
    26581 gcgccaaaac caatgtaatt ttagcacagc taggcacttg cactgggtta tgcgctggct
    26641 gctttctgag gtagatttaa gtgatcgccc ttagcttcgg acaacgcgta aatctctcta
    26701 accttttcgt ctggcaaatt accaaggctt aacgctatgg ctctacgaac tccaactaga
    26761 gcgtgcaact cttggatgtg ctcatccaga agaatgagta attgctccgc tccttttctc
    26821 ccaagaacat cagctacttg ttgtttttct tgtggtttaa gaaatgacaa gaacattaga
    26881 atttgagcat caacattatc cgcattctct tttttgggtt ctttggaatc ttttagttca
    26941 acatcaccct ctcccgtcat gagccattcc aaggtaacat tttcgctttc tgctatcttt
    27001 tttaactgat acgggcgtgg tatcggtgcg taatcccgcc tgttgtaata attttttaaa
    27061 gtgccttcat tgatccccca tgcccgagca gccgcatttc ttgaagggta tcgttctact
    27121 aacctaaaga ttctttcgat aatcggttct tttccatcgg caggaaaaga accttggggt
    27181 gcttttctca ttcttggttc ctttctatct aattcattga taaataataa aattaaaaaa
    27241 taaaccataa aaacaccaaa gaaccctttg cagggaatct ttggtatctt aatctttatc
    27301 catacggata tccgaccgga tatccgcgcg ggtaattttg taggttaaag gaaagatgag
    27361 acaaagagaa gatagtgacc gcgattggcc ttcgctgaag attcagctgg cgctcaaaga
    27421 taacgggtgg tcttccttcc gggagctttc actccatgct ggttatgctc aggattcact
    27481 gaaagcggta gttcgcacac caaacaaaaa atacgaacgc gtaatttctg acgctatagg
    27541 tgtaccgcca gaaatcatct ggcccagccg gtatcgtaac gatggattta tcaggaaggc
    27601 gtcgtgatat gcagtcgtac gttaccgtaa atgacctgct cggcgtacca ggaatgccag
    27661 ccaccacaaa aggcatccga caggcgttac agcgtttttc tcgtgatctt ggtgacgtta
    27721 gtcgccgtcg ggaaggcaca aaagccattg agtaccacat tgattgtctg cctgaaataa
    27781 cacgaaaggc actgcgtgaa cgctatgtgg aacagctggt ggctacagag aacaatgttt
    27841 ctgaagtgaa agctgttacc agaaaaacac gcaatcctga cgctgtccag gcaatcgaag
    27901 catatcgcgg ttctccacaa ctgatggaag aacgcctgaa tgcgctgacc gaaaaccagc
    27961 gctgggtatc tgaagcaaga gctgcgctgg tggtggaagt gctgaagctg gaaagcgccg
    28021 gtaaccccgg gcgactgaaa gccattaact ttcttgttga aaaagcccgt aaaggtgagc
    28081 tgccggagcg cctgcaacag gccgcagtta acgccaatgc aaaacgtggc gctaatcgta
    28141 ctatcagccg cgatccactt taccagtggg ttctgaaata caaccagtcg cagaacgccg
    28201 ccgaacggct gctgttactg gcaccgggaa aacgcgacga aataaaaccg gaagaaatca
    28261 gctggctgcc tgaatttctg gcgcagtatc gccaggtgaa cggcagaccc atgtcagaag
    28321 cctacgagga ttttgtcgca gaatggcagc gtcgccatgc ggacgaaccg tacatgctgg
    28381 aggtgatgcc ttcctatgat gttgtccggt atgccatgaa aaaactgccg gaagtggtga
    28441 aacaaaaagg gcgcgttacc ggcagtgaat accgtcagct tgaaggattc acccgccgcg
    28501 actggacagc tatgccggtg aattatgtct ggattggtga cggtcacggt atgaagctga
    28561 aatgtgcgca tccgatccac gggcgtccat tctcaccgga agtcacgttt gtgatcgatg
    28621 gtggaacacg gtttgttgtt ggctggagtc ttgaccttgc cgaaaacgtg ttcgccgttg
    28681 ccggtgcgat acagcacggc attcgcaacc acggaaaacc gttcctgtat tactcggata
    28741 acggttccgg tgaaacggct gacatgctgg acaaagaggt cgtgggtatt ctgccacgcc
    28801 tggggattaa acaccctacc ggtattgccg gtaatccaca gggacgcggc attattgaac
    28861 gcctgaaccg gacactgccg atgcgtatag ccaggaggta tcgcacctat tttggcaagg
    28921 gtgctgaccg ggaatcgttg cgtgtactta accgtgatct gcgttcagcg tttaacgccc
    28981 tgcaacagga caagcctctg aacgaccgcc agaaagcggc aatgcgcgag ctgccatcgt
    29041 gggcggagct tattgaggct attcgtgaag gcgtggaatg gtacaacaac cgcccgcatt
    29101 ctgagctgcc gatgaaaccg aacggacggc attacagccc gacagagttc agaaaaaaac
    29161 gccaggcaga agaagacacc gaaattgagt ggctgagtga tcttgagctg cgcgacatgt
    29221 tccgcccaat ggtggaacga ccggtaagac gctgcgaaat tcagtggctg aacaatattt
    29281 actacgcgcc ggagttgcgt gacgaacacg gtcgcaaagt gcttatcagt tatgacattc
    29341 atgacgccga gcgaattacg gtacgtcgca aggacggcag ctttatctgt gaggcgatat
    29401 ggaacggcaa taaacgcgca gcctttgctg tcagtgctga atatcacaaa caacagcagc
    29461 gtattaaagg aatgcgcaaa cgcgctgagg aaaaaattcg tgatgctgaa gatgaaggta
    29521 ttcagattct ggaacacaaa caggctgaac cctggctgag taatgtttat cgcccggttg
    29581 gtaatgtggt ggcagttcag caaccggaat atgaagaaga gcatgacgaa gaatttgaac
    29641 gcgatttccg gctggggatg caaaaacttt ttgccatgca ggaagaagat gatccgctgg
    29701 cctgaataaa aaaacagtcc gcgggaacgg actgttaaat aaaggaaaca aagctgattt
    29761 gaaataaccg aggaaatact atgactgata ttgatga
//
LOCUS       DAJTSA010000043        29467 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000043 DAJTSA010000000
VERSION     DAJTSA010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.043"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            71..352
                     /locus_tag="OO369_002070"
     CDS             71..352
                     /locus_tag="OO369_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR5982346.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
     gene            318..1395
                     /locus_tag="OO369_002071"
                     /pseudo
     CDS             318..1395
                     /locus_tag="OO369_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(1788..2129)
                     /locus_tag="OO369_002072"
     CDS             complement(1788..2129)
                     /locus_tag="OO369_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR5982347.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(2142..3014)
                     /gene="copD"
                     /locus_tag="OO369_002073"
                     /pseudo
     CDS             complement(2142..3014)
                     /gene="copD"
                     /locus_tag="OO369_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            complement(3018..3392)
                     /gene="yobA"
                     /locus_tag="OO369_002074"
     CDS             complement(3018..3392)
                     /gene="yobA"
                     /locus_tag="OO369_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR5982348.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3531..3761
                     /gene="holE"
                     /locus_tag="OO369_002075"
     CDS             3531..3761
                     /gene="holE"
                     /locus_tag="OO369_002075"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR5982349.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3863..4519
                     /locus_tag="OO369_002076"
                     /pseudo
     CDS             3863..4519
                     /locus_tag="OO369_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            4543..5205
                     /gene="exoX"
                     /locus_tag="OO369_002077"
     CDS             4543..5205
                     /gene="exoX"
                     /locus_tag="OO369_002077"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR5982350.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(5202..7262)
                     /gene="ptrB"
                     /locus_tag="OO369_002078"
     CDS             complement(5202..7262)
                     /gene="ptrB"
                     /locus_tag="OO369_002078"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR5982351.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7472..8131)
                     /locus_tag="OO369_002079"
     CDS             complement(7472..8131)
                     /locus_tag="OO369_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR5982352.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(8458..8814)
                     /locus_tag="OO369_002080"
     CDS             complement(8458..8814)
                     /locus_tag="OO369_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR5982353.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            complement(8881..9171)
                     /gene="yebG"
                     /locus_tag="OO369_002081"
                     /pseudo
     CDS             complement(8881..9171)
                     /gene="yebG"
                     /locus_tag="OO369_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            9305..10483
                     /gene="purT"
                     /locus_tag="OO369_002082"
     CDS             9305..10483
                     /gene="purT"
                     /locus_tag="OO369_002082"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR5982354.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10539..11180)
                     /gene="kdgA"
                     /locus_tag="OO369_002083"
                     /gene_synonym="eda"
     CDS             complement(10539..11180)
                     /gene="kdgA"
                     /locus_tag="OO369_002083"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR5982355.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(11217..13028)
                     /gene="edd"
                     /locus_tag="OO369_002084"
     CDS             complement(11217..13028)
                     /gene="edd"
                     /locus_tag="OO369_002084"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR5982356.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(13263..14738)
                     /gene="zwf"
                     /locus_tag="OO369_002085"
     CDS             complement(13263..14738)
                     /gene="zwf"
                     /locus_tag="OO369_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR5982357.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            15076..15945
                     /gene="hexR"
                     /locus_tag="OO369_002086"
                     /gene_synonym="yebK"
     CDS             15076..15945
                     /gene="hexR"
                     /locus_tag="OO369_002086"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR5982358.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            16073..17515
                     /gene="pyk"
                     /locus_tag="OO369_002087"
     CDS             16073..17515
                     /gene="pyk"
                     /locus_tag="OO369_002087"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR5982359.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17646..18617)
                     /gene="lpxM"
                     /locus_tag="OO369_002088"
                     /gene_synonym="msbB"
     CDS             complement(17646..18617)
                     /gene="lpxM"
                     /locus_tag="OO369_002088"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5982360.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(18737..20059)
                     /gene="mepM"
                     /locus_tag="OO369_002089"
     CDS             complement(18737..20059)
                     /gene="mepM"
                     /locus_tag="OO369_002089"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR5982361.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(20075..21007)
                     /gene="znuA"
                     /locus_tag="OO369_002090"
     CDS             complement(20075..21007)
                     /gene="znuA"
                     /locus_tag="OO369_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR5982362.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            21086..21841
                     /gene="znuC"
                     /locus_tag="OO369_002091"
     CDS             21086..21841
                     /gene="znuC"
                     /locus_tag="OO369_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR5982363.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            21838..22623
                     /gene="znuB"
                     /locus_tag="OO369_002092"
     CDS             21838..22623
                     /gene="znuB"
                     /locus_tag="OO369_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR5982364.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(22770..23780)
                     /gene="ruvB"
                     /locus_tag="OO369_002093"
     CDS             complement(22770..23780)
                     /gene="ruvB"
                     /locus_tag="OO369_002093"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR5982365.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(23789..24400)
                     /gene="ruvA"
                     /locus_tag="OO369_002094"
     CDS             complement(23789..24400)
                     /gene="ruvA"
                     /locus_tag="OO369_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR5982366.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            complement(24539..24604)
                     /gene="yobI"
                     /locus_tag="OO369_002095"
     CDS             complement(24539..24604)
                     /gene="yobI"
                     /locus_tag="OO369_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR5982367.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            24674..25276
                     /locus_tag="OO369_002096"
     CDS             24674..25276
                     /locus_tag="OO369_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR5982368.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(25278..25799)
                     /gene="ruvC"
                     /locus_tag="OO369_002097"
     CDS             complement(25278..25799)
                     /gene="ruvC"
                     /locus_tag="OO369_002097"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR5982369.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(25834..26574)
                     /locus_tag="OO369_002098"
     CDS             complement(25834..26574)
                     /locus_tag="OO369_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5982370.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(26603..27055)
                     /gene="nudB"
                     /locus_tag="OO369_002099"
     CDS             complement(26603..27055)
                     /gene="nudB"
                     /locus_tag="OO369_002099"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR5982371.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(27173..28945)
                     /gene="aspS"
                     /locus_tag="OO369_002100"
     CDS             complement(27173..28945)
                     /gene="aspS"
                     /locus_tag="OO369_002100"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR5982372.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            29255..>29467
                     /locus_tag="OO369_002101"
     CDS             29255..>29467
                     /locus_tag="OO369_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR5982373.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
BASE COUNT         7226 a         7568 c         7236 g         7437 t
ORIGIN      
        1 tattaaaaat caaccacacc cgccagtatt ctgtatattt actggcggtc atatcgtgag
       61 aggtatggca atgaatcttg tgacactcaa aacgtgggga aaactcagat atccggataa
      121 cccaccatca atatcaacgc tgagacgatg ggcaaggaat ggaaacattt atcctgcacc
      181 tgaactacac gggaggagtt acagggtggt tccggaggct ttctatatca acccaaataa
      241 ggttgatacc gatataacac accatcagcc taatgggcga caagggagag acagtccgtt
      301 aatggagaag ttaaaacatg cagcggaaaa aatacgatcc caatttgcct agaaacttaa
      361 catatcgaag gagagacaaa gcatattact ggcgcaaccc tctgacgaaa gaagaattta
      421 cactaggtaa aatttcaaga agagatgcag tagcgcaggc aattgaagca aatcattata
      481 tatacaaaaa ctactctcct gctgccctaa ttgaaaagct taaaggggtc gactcattta
      541 ctatggcagg ctggattgaa cgttacaaaa cgattcttat aaggagaaaa gcgtccagaa
      601 atacttataa aactcgggta aatcaactgg agacaataaa agaaaaatta ggaggggttt
      661 tactgacaga aataaccact cgccatatcg ccgagtttct tgatttgtag attgaaggag
      721 ggaaaaacac gatggcagga tcaatgcgtt ctgtgttatc tgatatgttc cgcgaagcca
      781 ttgttgaagg acgtatatct caaaatccag taacgccaac aagagcaccg aaaatagtag
      841 ttacaagaga acggctgaaa ctaaagacat acaactgcat cagggaggca gcagatcaac
      901 ttccggcatg gttcccatta gctatggact tagcccttgt aacaggacaa cgtcgcgaag
      961 acataacgaa tatgcggttc agtgatattt atgatgatcg tctccacatc aggcaaatta
     1021 agacaggaat gatgattgct atccccctgt cactcagcct tcctgtcgct ggtttacgac
     1081 ttggtgcagt agttgaacag tgccgcatgg taagtagggg ggattatcta atcagtgccg
     1141 ggattagaaa aaacagccct gacggcagca ttcacccgga cggcctgaca aagaaatttg
     1201 tcgcagccag aaaattaaca ggtatcactg ttcagtgaaa acccaccaac ttttcacgaa
     1261 atcagaagcc tggctggacg attgtacaaa gaaatatgtg gagaagaatt tgctcagcgt
     1321 ctacttggcc acacatcgga gaagacaaca aaaatgtatc ttgatgagag agaaaaaacg
     1381 tacttactgc tctgatttta acgtaaatgg attgttaaat gtattttggt tgtgatataa
     1441 ccaaaaaaga ccggaataca gaaattcgag taaatttcgg ggaatttcgg ggagacgttt
     1501 gcaactaatt gattttaaat acaattaaaa aaagaccgaa tacgattcct gtattcggtc
     1561 cagggaaatg gctcttggga gagagccgtg cgctaaaagt tggcattaat gcaggcttag
     1621 ttgccttgcc ctttaagaat agatgacgac gccaggtttt ccagtttgcg tgcaaaatgg
     1681 tcaataaaaa gcgcggtgct catcagctta aatgttaaaa accgcccgtt ctggtgaaag
     1741 aactgaggcg gtttttttat tggaaatcaa aaggctattt taggtaatta acagagtttt
     1801 tcagctcgtt ctataaacgg tgccagactc attttttcgc cgggattgtt aggatcatca
     1861 atctgaatca ccgaaatggg ttgggcattg gtcttcccac tggcaacttc cttttgtgcg
     1921 atatcgttta aaggatactg cacgagggta cttgggttga tgacatacaa agcattaccc
     1981 ggtcggcaag tcagcattac ctcttcgcga ttaaacgccc atttgtcttt acccacttca
     2041 aaacgactca cggttatcac ctgtggagcc gccagcgccg ctgcagaact ggtgagtaac
     2101 agaaacgcca gaatactttt tttcatcatt ttcgtaatcc atcaaaaagg ttcgagagtc
     2161 gcaagcaggc tgatgatcac cagcactacc gcacctatcg cccattcgag tttagtcatc
     2221 cacacaaacc agggtgccgc acgatcttca tcctgcctca tgcgcggtac aagaacataa
     2281 cgattagcca gcgcaatgac caccataatc atcaccagga tggctttgag caataagagt
     2341 tggccccagt aagtcgtaag cgtgggggga aatccggtaa tcaacaatgc attaagcacg
     2401 ccgcttgcca gtacgccgat caccgcaaaa tgcccgcacc aggaaaaacg catcagcgcc
     2461 tgaatagcct agtgtcgcca acggcctttg atgagttgca tacaccagag caccggcagt
     2521 aatcccccaa accacgccgc cgcacaaatc aggtgaatcg cgtgattagt ctgatggatt
     2581 ttcgctgtta ccccttcatt cagcgtcgca taccccactc ccgccagcag aataaattgc
     2641 gcggtagtga gcataaacag caaacgtggc atattccgcg gttgcataag ggcgacgatc
     2701 aacgttacga gggcgagaac aatttgccat aaccagacgc caccaaactg cgtttgcaaa
     2761 acagcctgcc aaatgtttgg cgaaaataca tctgtccatc ccgttcccat caacccgccc
     2821 tgaacggcga gcattgcagt ggcactgatc aaactccaga cggcagcgtg ctgttgtaaa
     2881 cgcagagaac gcttcgtcag caagcgacga atcattaagg gggccagcca ggcaccatac
     2941 attgcaaagc caaagaccag catcaacgag gtgaaatgga taaatcgcag cgcgatccag
     3001 gtaaacgcca gcatgattta tttcacgcta aaggtgtagt gtcctttcgt tttatgcccg
     3061 tccaccgaaa caacatgcca gtcaacggtg taggttccgg gtttcagcga atcggccagt
     3121 ggaacaatca gttgcttttg gtcctgctca ttccgcttcg ctggtaatgt tttaatattt
     3181 tcgttttttg gccccgtgat ttttgcacca ctgaatcctg tttcaacgcc ttccgagaag
     3241 tttaaagtga ttgcctgcgg ggcagctgtc acttgcgcgt ttgccgcagg atactgatgc
     3301 gttaaatgcg catgtgccca gacggaaggt gtcaccaacg aagtggtcag gattgccagc
     3361 gcgtagcgaa gggagcgtgc agttgaagcc atattatcta ttcctttttg taataacttt
     3421 tttacagagc ataaccttgt ctaatgtctg agtcgaggat catcaattcc ggcttgccat
     3481 ccaggctcac tcttagtaac ttttgcccgc gaatgatgag gagattaaga atgctgaaga
     3541 atctggctaa actggatcaa acagaaatgg ataaagtgaa tgtcgatttg gcggcggccg
     3601 gggtggcttt taaagaacgc tacaatatgc cggtgatcgc tgaagcggtt gaacgtgaac
     3661 agccagaaca tttgcgcagc tggtttcgcg agcggcttat tgcccaccgt ttggcttcgg
     3721 tcaatctgtc acgtttacct tacgagccca aacttaaata aaacttatac agagttacac
     3781 tttcttacat aacgcctgct aaattatgag tattttctaa accgccccca taatttgcag
     3841 tcattttgaa aaggaagtca ttatgtcgtt ctggaaagtt gcagcagcgc aatatgagcc
     3901 tcgtaaaacc tcactcaccg aacaggtagc tcatcatctg gagtttgtga gggccgccgc
     3961 caggcaacaa tgtcagctgt tagtttttcc atcgctttct ttactggggt gtgattattc
     4021 gcgacgtgcc cttcctgccc cacccgatct ttcactgtta gacccgcttt gttatgccgc
     4081 aacgacctag cgaatgacca tcattgccgg ccttcccgtt gaatataacg atcgctttat
     4141 tcgtggaatt gcggtgttcg caccgtggcg aaaaacgcct gggaactacc atcagagtca
     4201 tggtgcatgt ctgggccgtc gttccagaac catcacggtg gtcgatgaac aaccgcaagg
     4261 tatggatatg gacccgacct gttcactgtt tacgacaggg caatgtctgg gagaacccga
     4321 cctgctggcg tcggcccgtc gcttacaatt tttttcacat cagtactcga ttgccgtgct
     4381 gatggcaaat gcccgtggta acagtgcact atgggatgaa tatggtcgtc tcatcgttcg
     4441 cgccgatcgt ggttcattat tgttagtcgg tcagcgttca tcacagggtt ggcaaggcga
     4501 tatcattcca ttacgctagg ctttttcggc ctggatcatg ccatgttgcg cattatcgat
     4561 acagaaacct gcggtttgca gggagggatc gttgagattg cctctgttga tgtcattgac
     4621 ggaaaaatcg tcaaccccat gagcctcctg gtgcgccccg atcgtcctat tagtccacaa
     4681 gcgatggcga ttcatcgcat caccgaagcc atggtcgccg ataaaccgtg gattgaagat
     4741 gtgatcccac actattacgg tagtgaatgg tatgtcgcgc acaacgccag ctttgaccgc
     4801 cgcgtactgc ctgagatgcc cggtgagtgg atttgcacta tgaaactggc ccgtcgtttg
     4861 tggccagggc tcaagtacag caatatggcg ttatataaaa cacgcaagct caatgtacag
     4921 acaccgctgg gcctgcatca tcaccgcgcg ttgtatgact gttatatcac cgccgcgttg
     4981 cttatcgata ttatgaacac ctccggctgg acggcagaac agatggccga tatcaccgga
     5041 cgtccgtcgt tgatgacaac cttcaccttt ggcaaatacc gtggcaaagc ggtttccgac
     5101 gttgccgaac gctatccggg ctatctgcgc tggttattta ataacctgga cagcatgagc
     5161 ccggagctgc gtttaacact gaaacattat ctggaaaata cttaatccgc aggcgtagcg
     5221 ggtaatgttc cctgcgccag cgcgaccaga aaagcatatt ccatcgctac gccttcgtac
     5281 gatttaaagc ggccagattt accgccatgg cctgagtcca tgtcggtaca gagcaataaa
     5341 agatggtcat cggttttcag ctcgcgcaat ttagcgaccc attttgccgg ttcccaatat
     5401 tgcacctgag aatcgtgtaa accggtcgtt accagtaaat gcggataagc ctgtgcggtg
     5461 acgttgtcat acgggctgta gcttttcatg tactcgtaat attgcggatc ctgcggattt
     5521 ccccactctt caaactcacc agtggtaaga ggaattgatt catcaagcat cgttgttaca
     5581 acatcaacaa acggtacctg ggcgataacg ccgtggaata attccgggcg ttgattaatt
     5641 gcaacgccca tcaacatgcc ccccgcactt ccgcccatcg cataacagag cgaaggagag
     5701 ccatagccca gttttaacaa tgcatcgcag gcatcaagat aatcattaaa cgtatttttc
     5761 ttcttcagaa atttaccgtc ttcgtaccat tgttgcccca gctcaccacc gccgcgaaca
     5821 tggacaatgg cgtagacaaa gccacgatct aacaaactca agcggctaaa actgaaatcg
     5881 gcatcaatac ttgcgccgta agaaccatag ccatacacca gcaacgggtt gtgtcctttg
     5941 cgaaaatgtt tgcgatgata gaccagcgaa accggaactt cgacgccatc acgggcgact
     6001 atccacaggt gttcactgcg gtaattcgcc gcatcaaaac caggcacttc tgtttgtttt
     6061 aatacacgac gctcaccggt atccatatcc agttcaaaca aagtgtctgg tgtggtcatg
     6121 gaagaataac cataacgcaa tcgcgcggtt tcaggttctg gattgtaggc aatccaggtc
     6181 acataggccg gatcatcaaa ggcaatgcca atgacttccc gggtcttgcg gttaatttgg
     6241 cgcaaactgg ttaacccgcg ctgacgctct tcaaccacca gccagtcggt aaacagcgta
     6301 aacccttcca gcatgatgtt ttcgcgtggc ggaattaact cttcccactg ttgctcatca
     6361 cgcatacggg tacggtataa gccaaagttt ttgccgtggc gattggaacg cagataaaaa
     6421 cgatgctggt agtgatcaag gctgtattcg tgatctttgc ggcgcggcag aaaaacaaac
     6481 ggctcggcat cggccatttc cgcgtccagc aggcgaactt cactggtggt ggcgctggcc
     6541 agatgaatga cgacatagtg cttcgacgtc gttttatgca ggctgacata ataggtatcg
     6601 tctttttctt cgtagatcag tgtatcttgc gatgctggcg taccgatggc gtgacgccag
     6661 acctgataag gcagcagcgt caccggatgc ttgcgaacat agtagaaagt ccaggagtca
     6721 tttgcccaga caaagctggg ttcaacgtta tccagcagtt ccgggtacca gttacctgtt
     6781 tccagattac gaaaacgaat gccgtactgg cgtcgggaaa gaaaatcttc tgccagcgcc
     6841 ataatggtgt tatcgggcgt aatcgccatt ccgcccatcg aataaaactc actatgagct
     6901 gcgcgcttgt tggcatcgag caatgtttcc cactcatccc actcttcgct gaatgccgat
     6961 tgacgctggt agatagcata ttcacagcct ggttcataaa tatgccgata gcggtagcca
     7021 ttttttatgt agggcgcaga aacttctctt tgcggaatgc ggtcgatgat ttcctttaag
     7081 atgcgatcct gcaaggcttg ttgtgaggcc atcacccgat gaccgtaact attttcttgt
     7141 tgcaggtagt ccaggacttt tggctgagaa cgcgtatcgt cccgcagcca gtagtaatta
     7201 tcgatgcgcg tatcgccatg aagcgtcatg gcgtggggaa tgcgggcggc ttttggtagc
     7261 atgttattgt tctttctggt tggaaacatc ttataagggt ggcaaaactc accggggatg
     7321 caagcgaaac agggtcgtca ttgcttagat gatgacaggt aatggcgcgg atatcgaatg
     7381 ttatgcagac agagaaaatc agcctgttca aatggctgtg cggttctgga tagcccgaaa
     7441 tagtcaactt caggctatcc agagagcgga attattccgc caaagtgcgt ttttgctgtt
     7501 cgagatcgcg ttcaatgcct tcacgaacat cctgggggat tttcagcgcg tcacccagtg
     7561 cattcaggta actgcgttcc ataaaatggt caatatcaat agccgcgcag ctcaggaaat
     7621 agatttccag cgcctcttct tcattgcgga ccccggtagc caggcgttgt ggatccagcg
     7681 gttgttcgat tgcctgctca atgagtacac gcccctgctc ttccacgccg gcttcacgca
     7741 attgctggtc gattgccgca cgttcattgg catcaatatg accatcactt ttagcggcaa
     7801 agaccagcgc aaggatcaaa cgttccgtac gctcatccag cggcgtactt tgcgcgccaa
     7861 actgcggttc gtcctgatgc gccgcgcgaa ttttatcttt gtatttattc cacagcaccg
     7921 tacccgctac cgctccgccg ccaaccagta acgcgttggt gccatatttc gtaagaagtt
     7981 tacgtgctga tttatttgcg accagcagcc cagccagtcc gcctaatgcg cctgacacta
     8041 acagtttgcc caatccctga tccgcagacg aggaggtaga agaactgctt tgcccaagca
     8101 gggattgcag ttgatttaac cagttagcca tatttgctcc tcaataacca tttttagtgc
     8161 ctgtagcata gcggatgagg atgtcagaaa atgtatatcg agacaaaaga tgcgcaaata
     8221 agcggggcgg taggccgtta ttcaaagaaa ttcgcgccag gtaaggtatt tactgtatat
     8281 caggtgataa gggtatttta ggtgaaagta gtttgtcgtt ttgagtggca ttgtttgatg
     8341 ctgattgccc gatgcgacgc tgttgcgtct tctcaggcct acgattgtgg tgcgttctgg
     8401 gacggacaag gcactcgcgc cgcatccgcc aacggtcaat cgaattatca gaagccatta
     8461 acgccgctga tattccgcca ttcccgcttt gcagtccacg cagacctggt aatgaatatc
     8521 ggcactttta ccacgcacgg ttagcggtac tgaccattta tcatctttac cctgaatgtc
     8581 ctgcaaactg acccaggcca cgggatcggc ctgaccgaca attttttgat catctgccca
     8641 gcgcgccacg cgattttgtt gataatcacg ttttacgctc gcggcaattc cggcagcatc
     8701 cagaccttca cactttggga aagtgaccga cttgctggtt tcattattgg cagcgaaaac
     8761 tgatgcgcag gcagaaacca acaaccgccc taaaaacgcc cctctttttt tcatgttttt
     8821 ctccatagca caatgattca ggagaaagca tggtacaaat tgtcaggagc gcaagttgct
     8881 tcaggccgcg tgtgaggcat cttcctcttc ggattcagca ccgacgacct gtggggacgg
     8941 taatttgccg gttttcagaa tggtgctcag cacctctttt tgttctgcca gccatagcga
     9001 aagggcttca cgttgctcgt cttccatttg cactggagaa tttgtcagct aggtgtcaag
     9061 cagatccgcc gtatcaagca ttttgtcata agcatcggct tcctttttgc tggtaaacga
     9121 cattttctct tcgccctcac gaatgactac gtatttaact tcaaccgcca tttgcagcct
     9181 ctcataataa ctgagatttt atacagtata tttcttttcg gttgagaaat caacatcagc
     9241 aataaagaca cacgcaaacg ttttcgttta tactgcgcgc ggaattaatc aggggatatt
     9301 cgttatgacg ttattaggca ctgcgctgcg tccggcagca actcgcgtga tgttattagg
     9361 ctccggtgaa ctgggtaaag aagtggcaat cgagtgtcag cgtctcggcg tagaggtgat
     9421 tgccgtcgat cgctatgccg acgcaccagc catgcatgtc gcgcatcgct cccatgtcat
     9481 taatatgctt gatggtgatg cattacgccg tgtggttgaa ctggaaaaac cacattatat
     9541 cgtgccggag atcgaagcta ttgccaccga tatgctgatc caacttgaag aggaaggact
     9601 gaatgttgtc ccctgcgctc gcgcaacgaa attaacgatg aatcgcgagg gtatccgtcg
     9661 cctggcggca gaagagctgc agctgcccac ttccacttat cgttttgccg atagcaaaag
     9721 ccttttccgc gaggcggttg ccgccattgg ctatccctgc attgtaaaac cggtgatgag
     9781 ctcttccggc aaggggcaga cgtttattcg ctctgcagag caacttgctc aggcatggga
     9841 atacgctcag caaggcggtc gcgccggagc gggccgcgta attgttgaag gcgtcgttaa
     9901 gtttgacttc gaaattaccc tgctaaccgt cagcgcggtg gatggcgtcc atttctgtgc
     9961 accagtaggt catcgccagg aagatggcga ctaccgtgaa tcctggcaac cacagcaaat
    10021 gagcccgctt gcccttgaac gtgcgcagga gattgcccgt aaagtggtgc tggcactggg
    10081 cggttatggt ctgtttggtg tcgagctgtt tgtctgtggt gatgaggtga ttttcagtga
    10141 ggtctcccct cgtccacatg ataccgggat ggtgacgtta atttctcaag atctctcaga
    10201 gtttgccctg catgtacgtg ccttcctcgg acttcctgtt ggcgggatcc gtcagtatgg
    10261 tcctgcagct tctgccgtta ttctgccaca actgaccagt cagaatgtca cgtttgataa
    10321 tgtgcagaat gccgtaggcg cagatttgca gattcgttta tttggtaagc cggaaattga
    10381 tggcagccgt catctggggg tggtactggc tacttcagag agtgttgttg acgccattga
    10441 acgcgcgaag cacgccgccg gacaggtaaa agtacagggt taaacccggc aaaaaaacgc
    10501 tacaaaaatg cccgatccta gatcgggcat tttgactttt acagcttagc gccttctaca
    10561 gcttcacgcg ccagcttagt aatgcggtcg taatcgcccg cttccagcgc atctgccgga
    10621 accagccagg aaccaccgat gcacagcacg cttttcagtg ccaggtagtc acggtagtta
    10681 gccggagaaa taccacccgt cgggcagaaa cggacctggg agaacggacc cgcgatcgcc
    10741 tgcagggctt tcacgccgcc gttagcttca gccgggaaga atttgaactc tttcaaaccg
    10801 tagtccatac ccagcatcag ttcggaaaca gtgctgatcc ccggaatcag aggaatagtc
    10861 ccttcggtag cagctttcag cagcggctcg gtcagacccg ggctaattgc gaactgtgca
    10921 cccgcttcag tgacttctgc cagctgctgt ggattcagca ccgtaccggc acccacaatc
    10981 gcttcaggca cttctttggc gatagcacgg atagcgtcaa ctgcacactc ggtacgcaga
    11041 gtcacttcca gaacgcgcac cccaccagca accaacgctt ttgccatcgg caccgcgtgt
    11101 tccagttttt ttaccacgat aaccggtaca accgggccgg tggtcaggat tgattctgca
    11161 cttgttttcc agtttttcat cagagttttc tctcgcctga ttacaaattt gtcgtcttaa
    11221 aaagtgatac aggttgcgcc ctgttcggca ccggacagtt tttcacgcaa ggcgctgaat
    11281 aattcacgtc ctgttcccac gcgtgacgcg ctcaggtcag gaatgtgcgg ttcgcgagca
    11341 gccagttccg cttcgtctac cagcagtgtc agttcgcctg tctgtccatt cacacgaatg
    11401 atgtccccgt cgcgcacttt tgccagcagc ccgccatcgt aggcttctgg tgttacgtgg
    11461 atagctgacg gcactttacc tgaagcgccg gagagtcgtc catcggtaac taacgcaatt
    11521 ttgaaacacc ggtccaataa tacaccaagt ggcggcatga gtttatgtaa ttctggcatt
    11581 ccgttcgctt ttggcccctg atgacggaca acaacgacac aatcgcggtc cagcaaaccc
    11641 gcttcaaagg ccggcataac gtcatgctgg ctttcaaaaa caaccgctgg cgcttcaatc
    11701 acctggttct caaccggcac ggcagaggtt ttcataaccg cacggcccag gttaccgctt
    11761 aacacttttg tcccaccatg atgagagaaa ggttgttcga aggaagcgat cacattgctg
    11821 tcgagtgatt tttccgcccc ttcccgccag tccagttcac cattattcag ccatggttca
    11881 agggtataac gagacagacc aaagcctgcc accgtattga catcttcatg cagcaggcct
    11941 gctttgagca gttcacgcac cagaaccggt acgccacctg ccgcctggaa gtggttaata
    12001 tcggccggac cgttcgggta gagacgtgcc atcagcggta caacatcaga aaggtcagag
    12061 aagtcatccc agttaatctg aataccggcc gcgcgcgcca tcgccaccag gtgcatggtg
    12121 tggttagtgg aaccaccggt cgccagcagt gcaacgatac cgttcaccac cactttctca
    12181 tcgatcatct taccgatcgg catccattca ttaccattac cggtcatgcg tgtaacctga
    12241 cgcgcagcgg cagcggtcaa agcatcgcgc agcggagaat ccggatgaac aaaagaagag
    12301 cctggcaact gcatccccat aaattccacc accatctggt tggtgttggc agtaccgtag
    12361 aaagtacatg ttcccggcgc atggtaagac gcggcttctg actccagtaa ggccatgcgg
    12421 tccactttac cttcggcata aagctggcga atacgcactt tttctttatt tggcaaaccg
    12481 cttgccatcg gtccagacgg cacaaacacc gcaggcaaat gaccaaacga cagggctgcc
    12541 atcgtcagac ccgggacaat cttgtcgcac acaccgagga acagagcacc atcaaacatg
    12601 ttatgggaca gccccaccgc cgcagacatc gctatcactt cgcggcttag cagcgacaat
    12661 tccattccat cctgcccctg ggtgacacca tcacacatcg ccggaacacc gcccgcaacc
    12721 tgaccaaccg cattcgcttc atgcagggct ttacgaatga tttctggata gtgttcataa
    12781 ggctggtgcg cggagagcat gtcgttatag gaggtgatga tggcgatatt gttacgcaac
    12841 atgcttttca aagaggcttt gttttctggc tggcaggcag cgaaaccgtg tgccaggtta
    12901 ccgcatgcca actgcgaacg atgaacggtc gacgttttcg cttgttctat ccgggcgaga
    12961 taagcagagc gagtctcgcg cgaacgttca atgattcgat ttgttacgcg taacaattgt
    13021 ggattcataa aggctcctga aattgagttg tcagagcagg atgattcaca acgcgtttca
    13081 ttcagaggat ttatgagtga aacgcctgta accggagctc atagggcaaa aacgtttcag
    13141 tcagtgtaat aaaaaaagcc tcgtgggtga atccgcacga ggcctgaaag tgtaaaaatt
    13201 gttctacaat ctgtgcaaga tcatgttacc ggtaaaataa ccataaagga taagcgctga
    13261 tattactcaa actcattcca ggaacgacca tcacgggtaa tcatcgccac cgaggcaacg
    13321 ggtccccagg ttccggcctg atacggtttc ggcgcatcat tgtccatcgc ccacgcctca
    13381 gtaatggagt ctacccattt ccaggcttct tccacttcgt cgcgacgtac aaacagtgcc
    13441 tgaataccac gcatggtttc cagcagcaaa cgttcatagg catccgccag atgcgtctga
    13501 ttaaaggttt ctgaatagct cagatccagc ttggtgattt gcaggttatg tttgtggtca
    13561 aggccaggaa ctttattcag tacctggata tccacgcctt catcaggttg cagacggata
    13621 gtcagtttat tctgcggcag atcctgccac gactctttaa acagattcag ttcaggtgtt
    13681 ttgaaataga ccacgacttc agaacatttg gtcggcagac gtttaccagt acgcaggtag
    13741 aatggcacac cggcccagcg ccagttatca atgtcgacgc ggatcgccac gaaggtttct
    13801 gtattgctgc ttttgttcgc gccctcttct tccagatatc ccggcacttt tttgccctgg
    13861 gcgaagcccg cagtatattg cccgcgtacg gttttttcgc gtacgttgga gcggtcgatg
    13921 cggcgcaggg acttcagtac tttcactttt tcatcgcgga tgctgtctgc gctcaggtca
    13981 gatggcggag acatcgcaat catgcagaga atttgtagca ggtggttctg aatcatgtca
    14041 cgcatctgac cggctttatc aaaatagccc cagcgccctt cgatccccac ttcttctgcc
    14101 acggtaatct caacatgatc aatggtgcga ttgtcccagt tattcacaaa cagggagtta
    14161 gcaaaacgca gcgccaacag gttcagcacc gtttctttac caagatagtg gtcgatacgg
    14221 taaacctggc actcctcgaa gtattcgcca acctgatcat tgatttcctg cgaggtcgcc
    14281 agcgacgtcc ccagcggttt ctccatgact acgcgtgccg gtttagcatt cagttttgcc
    14341 tcgccaagcc ctttgcaaat tgcgccaaaa gtgctgggcg gcatggcaaa gtagttaata
    14401 gtgatacgat ttttttgatc cagcatcgcg ccgagacggt tgaatgcagc agtgtcattg
    14461 acatcgagat tacaaaaatc cagacgtgca ctcagggtgt cccataaacc ttcatcaatg
    14521 gtttctttca tgaaagtttc gagcgcctcg cggacaactt tggtatatgc cgctttatcc
    14581 cagtcagcac gccctacgcc gataatccgg gtgtccgggt tgagctgacc gactttttcc
    14641 agttgataca gggaaggcag caatttacga cgcgcaaggt cgcctttcgc gccgaaaatg
    14701 accaggtcac aggcctgggc tgtttgcgtt accgccatgt cattctcctt aagttaacta
    14761 acccggtact taagccaggg tatacttgta attttcttac ggtgcactgt actgctttta
    14821 cgagcttgcg aaaactgtaa acgcttatcc acccgtgcga ttacgggaaa agcgcgcaaa
    14881 gcgcggcaaa actgataaaa aaatcatcgt tttccattcg gtaaaaatct gacactgatc
    14941 atgttatgaa aaaaaataac aactttttta tctgcttttg tcattaacac ggcacacagg
    15001 cgtaatatct gacaaaactg catttcgatt tctttcagtg cggaaatcgt cattacccgt
    15061 gagtctcttt acatcatgaa tatgctggaa aaaatccagt ctcagctgga acatttgagc
    15121 aaatcagagc gcaaagttgc cgaggtcatt ctggcttcgc ccgataacgc gatccattcg
    15181 agtattgctg ctctggcgct ggaagccaat gttagcgaac cgacggtgaa tcgtttctgt
    15241 cgcagcatgg acacgcgcgg ttttcctgat tttaaacttc atctggcaca aagtctggcg
    15301 aatggcactc cctatgttaa tcgcaacgtc aatgaagatg acagcgttga atcatacacg
    15361 gggaaaatat ttgagtccgc aatggcaacg ctcgatcatg tccgtcattc actggataaa
    15421 tctgccatca accgcgccgt cgacttgctc actcaggcaa aaaaaatcgc ctttttcgga
    15481 ttaggctctt ctgccgccgt tgcccacgat gcgatgaata agttctttcg ttttaatgtt
    15541 ccggtggtgt actccgatga tatcgtgctg caacgcatga gttgtatgaa ttgtagcgac
    15601 ggagacgtgg tggtgctgat ttctcacaca ggaagaacaa aaaatctggt cgagctggcg
    15661 cagctggcac gcgaaaacga cgccatggtg attgccctca cctctgcggg taccccgctc
    15721 gcccgggaag caacgctggc aattaccctc gacgtaccgg aagatactga catttatatg
    15781 cccatggttt ctcgacttgc acagctgacc gtgatagatg tgctggcgac aggatttact
    15841 ttgcgacgcg gtgcaaaatt cagagataac ttgaagcggg tcaaggaagc gctgaaggaa
    15901 tcgcgttttg ataagcagtt acttaattta agtgacgatc gctaaaagcg actgtcactg
    15961 tcctaatctt atacgacatc cgaatgagat taatttatcg ccatcgcggc gttatttcat
    16021 tcggatttca tgttcaagca acacctggtt gtttcagtca acggagtatt acatgtccaa
    16081 aaggcttcgc agaacaaaaa tcgttaccac gttaggccca gcaacagatc gcgataataa
    16141 tcttgaaaaa gttatcgcgg cgggtgccaa cgttgtacgt atgaactttt ctcacggctc
    16201 gcctgaagat cacaaaatgc gcgcggataa agttcgtgag attgccgcaa aactggggcg
    16261 tcatgtggct attctgggtg acctccaggg gcccaaaatc cgtgtatcca cctttaaaga
    16321 aggcaaagtt ttcctcaata ttggggataa attcctgctc gacgccaacc tgggtaaagg
    16381 tgaaggcgac aaagaaaaag tcggtatcga ctacaaaggc ctgcctactg acgtcgtgcc
    16441 tggtgacatc ctgctgctgg acgatggtcg cgtccagtta aaagtactgg aagttcaggg
    16501 catgaaagtg ttcaccgaag tcaccgtcgg tggtcccctc tccaacaata aaggtatcaa
    16561 caaacttggc ggcggtttgt cggctgaagc gctgaccgaa aaagacaaag cagacattaa
    16621 gactgcggcg ttgattggcg tagattacct ggctgtctcc ttcccacgct gtggcgaaga
    16681 tctgaactat gcccgtcgcc tggcacgcga tgcaggatgt gatgcgaaaa ttgttgccaa
    16741 ggttgaacgt gcggaagccg tttgcagcca ggatgcaatg gatgacatca tcctcgcctc
    16801 tgacgtggta atggttgcac gtggcgacct cggtgtggaa attggcgacc cggaactggt
    16861 cggcattcag aaagcgttga tccgtcgtgc gcgtcagcta aaccgagcgg taatcacggc
    16921 gacccagatg atggagtcaa tgattactaa cccgatgccg acgcgtgcag aagtcatgga
    16981 cgtagcaaac gccgttctgg atggtactga cgctgtgatg ctgtctgcag aaactgccgc
    17041 tgggcagtat ccgtcagaaa ctgttgcagc catggcgcgc gtttgcctgg gtgcggaaaa
    17101 aatcccgagc atcaacgttt ctaaacaccg tctggacgtt cagttcgaca atgtggaaga
    17161 agctattgcc atgtcggcaa tgtacgcagc taaccacctg aagggcgtta cggcgatcat
    17221 taccatgacc gaatcgggtc gtaccgcgct gatgacctcc cgtatcagct ctggtctacc
    17281 aattttcgcc atgtcgcgcc atgaacgtac gctgaacctg actgctctct atcgtggcgt
    17341 tacgccggtg ccctttgata gcgctaatga cggcgtagca gctgccagcg aagcggttaa
    17401 tctgctgcgc gataaaggtt acttgatgtc tggtgacctg gtgattgtca cccaaggcga
    17461 cgtgatgagt accgtgggtt ctactaatac cacgcgtatt ttaacggtag agtaagtacg
    17521 ttgccggatg cggcgaaaac gccacatccg gcctatagtt caatgatagt tcaacagatt
    17581 tcgaatattc tgaagcaaac ttgaacttat catcaggcga aggcctctcc tcgcgagagg
    17641 cttttttatt tgatgggata aagatctttg cgcttatacg gctggatttc gcccggtttg
    17701 cgagttttca gcaattttag tatccaggtg tattgttctg gtcgcggacc aacaaaaatc
    17761 tcgacttctt cattcatccg ccgctcaatc gtatgatcat ccgcctctaa cagatcatcc
    17821 atcggtgggc gcacctgaat cgtcagacga tgcgtcttgc catcataaat cggaaacagc
    17881 ggtacaacgc gcgcacggca tactttcatc aaacgaccaa tcgcgggcaa cgtcgcttta
    17941 taggtggcaa agaaatccac aaattcgctg tgctctgggc catgatcctg atcgggtaaa
    18001 taatatcccc agtacccctg acgtaccgac tggatgaatg gtttaatacc gtcatttctc
    18061 gcatgcagac gaccgccaaa gcgacgacgc accgtgttcc agacataatc aaaaaccggg
    18121 ttgccctgat tatggaacat cgctgccatt ttctgccctt gcgaggccat cagcatggca
    18181 ggaatatcga cggcccaacc gtgcggcacc agaaagataa ctttctcgtt attacgccgc
    18241 atctcttcga tgatctccag cccttgccag ccaacgcgcg gctgaatttt ctccggcccg
    18301 cgtattgcca actcagccat cattgccatc gcttgcggcg cggtggcaaa catctcatca
    18361 acaatcgctt cgcgttcagc ttcactacgt tctggaaagc agagcgacag attgattaac
    18421 gcacgacggc gtgagctttt tcccagtcgt ccggcaaaac gtcccagccg tgccagaatg
    18481 ggatcacgga actttggcgg cgttaaagcg atacccgcca tcgctgctac acccagccat
    18541 gctccccagt agcgcgggtg gcgaaaggat ttatcaaact caggaatgta ttcgctatta
    18601 ttttttttcg tttccatgct tttccagttt cggataaggc aaaaatcaat ctggtgatag
    18661 tgtagcggca caacttgccc cgcaccaaat aaaaaaagcc ggtactgact gcgtaccggc
    18721 tgcgaatgga tgttaattaa tcaaaccgta gctgcggcac aatctctttg gcctgcgcca
    18781 ggaattcgcg acgatcggag ccggtcagcc cttcggtacg cggcagtttt gccgtcagcg
    18841 ggtttacggc ctgctggttt atccatactt catagtgcag atgcggccca gttgaacgtc
    18901 cggtattacc ggaaagcgcg atacggtcgc cacgtttcac cttctgtccc ggtttcacca
    18961 gaatcttgcg caagtgcata taacgcgtgg tgtagctgcg accatgacga atagccacat
    19021 aataacctgc tgcgccactg cgtttggcaa ccaccacttc accgtcaccc actgaaagca
    19081 ctggcgtacc ttgtggcatg gcgaaatcaa cacctctgtg tggtgcaacg cgacctgtca
    19141 ccggattagt tcgacgcggg ttaaagttag acgagatacg gaactgtttc gccgtcggga
    19201 atcgcaagaa tcctttcgcc agaccagtac cgttacgatc gtagaatttg ccatcctcag
    19261 cgcggattgc gtaataatct ttaccttctg aacgcaaacg tacgcccagc agctggcttt
    19321 gctcacgttt accatcaagc atttctcgag acattaatac cgcaaattca tcgccttttt
    19381 tcagtttgcg gaaatccatt tgccactgca tggctttaat cactgcgctc acttcggcgc
    19441 tggttaaacc ggcgtttctg gcgctggcaa caaagctccc tccgacggta cctttcagca
    19501 gattgttaac ccactctcct tgctgcattt cgctggtcat tttaaaaccg ttagcggcag
    19561 tacggtcata ggttcgggtt tcacgacgag acacttccca ggtgaggcgc tgcagttcgc
    19621 cgtccgcggt taatgtccag gagagttgtt gaccgatttt caggttacgc aattctttgt
    19681 cggcagcagc aagttgggtg atatcaccca tatcaatacc atactgattg agaatgctgc
    19741 ttagcgtatc gccagtggaa acaacatatt catgcacgcc cgcttcaccg gcgattttgt
    19801 catccagttc gtcctgggga atggcttcat cttcttgtgc agcttgatca atcggctcac
    19861 tggcttcagg taagagcgaa cgaatttcgt tctgttccag ctcaatggtt ttgacaattg
    19921 gcgtggcatc acggtgataa acatagggcc gccagacagc gacggccaga gtaagaacgg
    19981 tgagcgaccc caacataacg cggtgtggtc gcggtaaatt attaaacgcc agggcgacag
    20041 agcgggctat ctgttgcacg taatcacttc ctcattaatc tcctttcagg cagctcgcat
    20101 actggttggc taattgactc aggaattctg aatagcttgt tttacccagt ttgatattcg
    20161 tccccagggg atccaacgtt cccatacgaa cggatgtccc tcgtgcgacg ctctcaacga
    20221 ccgctggcct gaactgtggc tcagcaaaaa cgcaggttgc tttttgctca accaactgtg
    20281 ttcttatttc atgtaaacgc tgcgcgccag gttgaatctc agggttaacg gtaaaatgac
    20341 caagcggtgt cagtccgaac tgtttttcga aatagccgta agcatcgtga aaaacgaaat
    20401 aacctttccc cttgagcggc gcgagctcgt taccaacctg cgtttcggtt gaggccaatt
    20461 gtgcctcaaa atccttcagg ttggcgtcaa gtttggctcg actttgcggc ataagttcca
    20521 ctaattttcc atggattgca accgctgtag cccgcgctat ctctggggaa agccaaagat
    20581 gcatgttgaa atcgccgtga tggtgatctt cgtcactttt ttccgcgtgg tcgtgatcat
    20641 catcatcgcc gtgaatactt ttcatcagta gcggtttcac atcttcaagc tgcgcaatcg
    20701 ttacctgctt cgcttctggt aatttgctta ccggtttttg cataaacgct tccatctccg
    20761 ggccaaccca aacgactaag tccgcgttct gtaagcgttt tacatccgat ggacgcagtg
    20821 aataatcatg ttctgaagcg ccgtcaggca gtaaaacctc cgtttctgtt acaccatcag
    20881 caatggcaga agcgatgaac ccaacgggtt taagcgaagc gacaacggcg gcatctgcgg
    20941 cctgtgttgc acctccccag agagcggcgg ataatgctgc gaaaagaagc gtttttttat
    21001 gtaacataat gcgaccaatc atcgtaatga atatgagaag tgtgatatta taacatttca
    21061 tggctactgc aagactaaaa ttaacatgac aagtctggtt tccctggaaa atgtctcggt
    21121 ttcttttggc caacgccgcg tcctctctga tgtgtcgctg gaacttaaac ctggaaaaat
    21181 tttgacttta cttgggccaa atggcgcagg taagtcgaca ctggtacggg tagtgctcgg
    21241 gctggtaaca cccgatgaag gggttatcaa gcgcaacgga aaactgcgca tcggctatgt
    21301 accgcagaag ctgtatctcg acaccacgtt gccactgacc gtaaaccgtt ttttacgctt
    21361 acgccctggt acacataaag aagatatttt gcctgcactg aaacgtgtcc aggccgggca
    21421 cctgattaac gcaccgatgc aaaagctctc tggtggcgaa acgcagcgtg tactattagc
    21481 gcgagcattg ttaaattgcc cgcaattatt agtgctggat gaacccactc aaggcgtgga
    21541 tgtaaatggc caagtggcgt tatatgacct tattgaccag ttgcgtcgtg aactggattg
    21601 tggcgtttta atggtttctc acgatctgca tctggtgatg gcaaaaaccg atgaagtgct
    21661 gtgcctgaat caccacattt gttgttccgg cacaccggaa gttgtttccc tgcatccgga
    21721 gtttatttca atgtttggtc ctcgtggtgc tgaacaactg ggtatctatc gccatcatca
    21781 taatcatcgt cacgatttac agggacgaat tgttttgcgt cggggaaatg atcgctcatg
    21841 attgaattat tatttcccgg ttggttagcc gggatcatgc tcgcctgtgc cgcgggtccg
    21901 ctgggttcgt ttgtagtctg gcgtcgtatg tcttatttcg gtgatacgct ggctcatgcc
    21961 tcattacttg gtgtcgcgtt tggtttgttg ctggacgtga atccattcta tgcggtgatt
    22021 gccgttacgc tgctgctggc gggcggtctg gtatggctgg agaagcgtcc acagctggcg
    22081 atcgacacgt tattagggat tatggcgcac agtgccctgt cgctgggcct agtggtcgtt
    22141 agtctgatgt ctaacattcg tgttgatttg atggcttacc tgttcggtga tttactggca
    22201 gtgacgccag aagatctcat ctctattgcg attggcgtgg tcatcgtggt ggctattttg
    22261 ttctggcaat ggcgcaattt gctgtcgatg acgattagcc cggatctggc gtttgttgat
    22321 ggtgtgaaat tacagcaagt gaaattgttg ttgatgctgg tgacggcatt gacgattggt
    22381 gtagcgatga aatttgtcgg tgcgttgatt attacttcgt tgctgattat tcctgcagct
    22441 actgcgcgtc gctttgcccg tacgccggaa cagatggctg gtgtcgctgt tttggtgggg
    22501 atggtggcag taactggcgg tttaaccttt tccgcgtttt acgatacgcc ggcgggtccg
    22561 tcggtggtgc tatgtgcggc actgttattt attatcagta tgatgaaaaa gcaggccagc
    22621 taatctatcg ctgaacacat ttgtcggatg cggcgcgagc gccttatccg acctacggtt
    22681 cgctatctct ggctggcctg ataagacgcg aacagtgtcg catcagacac actgccagtg
    22741 tcggatgcgg cgcgagcgac caatccgact tacggcattt ctggcggcgt tatgccaaag
    22801 tgattccacg cccgcgtcgt cgccatacgc ccgcgcggtg tacgctgcaa aaagccttgc
    22861 tgaatcaaat aaggttccag tacatcttca atggtttcac gttcttcgcc aatggctgcc
    22921 gccaggttat ccagacctac cggcccacca aagaacttat cgattaccgc cagcaacaat
    22981 ttgcggtcca tataatcgaa accttcagca tcgacattca acatatccag cgcctgagca
    23041 gcgatatctg ccgagatggt gccatcgtgc ttcacttcgg cgaaatcacg cactcgacgc
    23101 agcagacggt tggcaatacg tggcgtaccg cgagcgcgac gagctacttc ctgcgcaccg
    23161 tcatcactca tctcaagccc cataaagcgt gcactgcgac tgacgatata ttgcagatcc
    23221 ggcacctgat aaaactccag acgttgcaca ataccaaaac ggtcgcgcaa cggtgatgtc
    23281 agcgaacctg cgcgcgtggt tgcgccaatc agggtaaacg gcggcaaatc aattttaatg
    23341 gagcgtgccg ccggaccttc accaatcatg atatccagtt ggtagtcttc cattgccggg
    23401 tacagcactt cttcaacaac tggcgataaa cggtggatct catcaataaa cagcacgtca
    23461 tgcggttcaa ggttagtgag catcgcagcc aaatcgcccg ccttttccag caccggacca
    23521 gaagtcgtgc gtaaattaac gcccatttca ttggcgacaa tgttggcaag cgtagtttta
    23581 cccaaccccg gaggaccaaa aatcaacaaa tgatcgaggg catcgccgcg cagtttcgct
    23641 gctttgatga aaatctccat ctgtgaacga acctgcggct gaccaacata ctcttccagt
    23701 aatttagggc gaatggcgcg atctgctaca tcttccggca aagtggtacc ggcagaaatc
    23761 agacggtctg cttcaatcat cctttacctc ataacgcggc gcgtagggct tcgcgaatta
    23821 atgtttcact gctggtgtca gggcgagcga ttttgctcac catgcggctt gcttcttgtg
    23881 gtttatagcc tagcgccacc agcgcggcaa ctgcttcttg ttcagcatcg tcggtcgccg
    23941 ggctagcagg agacgtgagt accaggtcgg cggctggcgt aaagagatcg ccatgcaaac
    24001 ctttaaatcg gtctttcatt tcaacaatca agcgttcggc ggtttttttg ccaatacccg
    24061 gcagtttcac cagtgccccc acttcttcac gctcaacggc attaacgaac tgctgcgctg
    24121 acattccgga gaggatcgcc agggccaact tcgggccgac gccgttggtt ttgatcaact
    24181 ctttgaacaa cgtgcgttct tgtttattgt taaaaccgta cagcagttgc gcgtcttcac
    24241 gcaccacaaa gtgggtgaaa acgatcgctt cctgacccgc ttcagggagt tcataaaaac
    24301 aggtcatcgg catatgcact tcatagccta cgccgcccac ttcaattaac accagcgggg
    24361 gttgtttttc aatgatgatg cctctgagtc tgcctatcac atgacgctcc tgcgtaatga
    24421 atcaaagata atgctgtatg ataaaaaaat gctggataga tatccagcga aggatgaaga
    24481 aaacttgcga ggtgtcccga tgatctgaaa aatggcacag tataatttat tcaacaggtt
    24541 atattggaag caaatatttg aatattacat attcagtgaa gaaatgcgta ataaaaatat
    24601 acattgcgac tcctgaaaaa taattttttt atgctattac gtatatttgt atctatttca
    24661 atggaatgac aacgtgaata ttaattaccc tgctgaatat gaaattggag atatcgtctt
    24721 tacatgtata ggtgctgcct tatttggtca aatatcagct gcatcaaatt gctggagtaa
    24781 tcacgtcggg atcattatcg gtcataacgg tgaagacttc ctggttgcag aaagccgggt
    24841 tcccctttca accatcacca cgctatctcg ttttattaaa cgttcgtcta atcaacgcta
    24901 tgctataaag cgattagacg ctgggctgac agaacaacaa aaacaacgaa ttgttgaaca
    24961 ggttccctcc cggctacgca aactttacca cacgggtttt aaatacgaat cttcgcgcca
    25021 gttctgttca aaatttgtct ttgatattta taaagaggcg ctatgtattc cggtgggtga
    25081 aatagagacg tttggacaac tgttaaatag caatccgaat gcaaaactca ctttctggaa
    25141 attctggttc ttaggttcta ttccgtggga gcgtaaaacc gtcacgccag ccagtttgtg
    25201 gcatcatccg ggtttggtgt tgattcacgc ggagggagtt gaaacgcctc agccggaact
    25261 gaccgaggcg gtataactta acgcagtcgc cctctcgtca ggttcagccg cgattcgctc
    25321 atctgcatcg cattctgact aacgtggcag tgggtgatgg caatcgccag cgcatcggcg
    25381 gcatccgcct gtggattagc gggcagtttc agcaaggtgc ggaccatatg ctgcacctgg
    25441 cttttttcgg cactacccat acctaccacc gtttgcttta cctgacgtgc cgcgtattca
    25501 aataccggca attcctgatt caccgccgca acaatcgcca cgccgcgcgc ctggcccagt
    25561 ttcagggctg agtcagcgtt cttcgccata aagacttgtt caatggcgaa ataatcaggc
    25621 tggaactggg tgatgatttc cgtcacgccc gcatagatga gcttcagacg agacggtaaa
    25681 tcatccactt tggtgcgtat gcatccgcta cccaggtagg acagttgcct acctacctgg
    25741 cggatgacgc cgtagccggt cacgcgcgaa cccgggtcaa tgccgagaat aatagccatc
    25801 acgcgtctcc gttttgctgt ttagcaggca tcatcagaga gtcgctgcaa cctcatcaga
    25861 gatttcaccg ttatggtaaa cttcctgcac gtcgtcgcaa tcttccagca tatcgatcag
    25921 acgcatcagt ttcggcgcgg tttctgcatc catatcagct ttggtggacg ggatcatgga
    25981 aacttccgcg ctgtctgctt tcagacctgc cgcttccaga gcgtcgcgca ctttacccat
    26041 ttcttcccat gcagtgtaga catcaatcgc gccgtcatca tatgtcacaa cgtcttcagc
    26101 acctgcttcc agagctgctt ccatgatggt gtcttcgtcg cctttctcga aggagatcac
    26161 gccttttttg ctgaacagat aagctacgga accgtcagtg ccgaggttac cgccacattt
    26221 gctgaatgcg tgacgcactt cagcaacggt acggttgcgg ttgtcagaca gacattcaat
    26281 catgattgcc gtgccgccag gaccgtaacc ttcgtagatg atggtttcca tgtttgcatc
    26341 atcatcaccg cccacaccac gtgcaattgc gcggttcagt gtgtcacggg tcatgttgtt
    26401 agacagtgct ttatccaccg ccgcacgcag acgcgggtta gcgtccggat cgccaccgcc
    26461 cagcttagcc gcggttacca gctcacgaat gattttagtg aagattttac cgcgcttagc
    26521 atcctgcgca gctttacgat gtctggtgtt ggcccattta ctatgacctg ccataaaaat
    26581 atctccagat agccctgcct gttcaggcag cgttaattac aaactgttca atcgcctgcc
    26641 ggttgctcca ggacttagtg agcgccgccg cagcaggcgc atcaagccac ttgtaagcca
    26701 gatgttcagt gaaaacgatc tgccgctcgt gcggaagcgc aagacagaac catgattccg
    26761 tattacgcgt cacgcccggc gcatagcgat gacgtaaatg tgaaaaaatt tcaaactcta
    26821 ccgtgcgctg acagtcaatt aaggtcagtt gttcagcgac aacatcaatg gtaacctctt
    26881 cctttacttc gcgcatggca gcttgcggcg cggtttcacc ctcttccacg ctgccggtta
    26941 ccgactgcca gaaatcggga tcgtcacgcc gctgcaacat cagcacccgt ttcgtatctt
    27001 gtgcatagat gaccactaag atcgaaacgg gacgcttata cactttatcc ttcacgctgc
    27061 ctcttcgttg actgccttcg ctcatcccat tcacatagtt atctatgctc atgggagttc
    27121 actcagttgc cgccttgatg caacgcgaat gatttcgtgt atttgagtca tatcagttat
    27181 tctcagcctt cttcacaacc tgaatgctca gctcagccag tgcagtcggg ttagcaaagc
    27241 tcggtgcttc agtcatcaga cacgctgccg ccgtggtttt cgggaaggcg ataacgtcac
    27301 ggatattgtc ggtgccagtc agcagcatgg tcagacggtc aagaccgaat gccagacctg
    27361 cgtgcggcgg agtaccgtat ttcagggcgt cgagcaggaa gccgaatttc tcgcgctgtt
    27421 cctcttcgtt gatacccaga ataccaaaca ccgtctgctg catatcacca ttatggatac
    27481 gtactgaacc accgcccact tcgtaaccat tgatgaccat atcgtaagcg ttcgccaccg
    27541 cattttccgg tgcagctttc agttctgcag cagtcatatc tttcggtgag gtgaacggat
    27601 ggtgcattgc cgtcaggccg ccttcaccgt cgtcttcaaa catcgggaag tcgataaccc
    27661 acagcggtgc ccatttgctt tcgtcggtca gaccaaggtc tttacccact ttcaggagca
    27721 gtgcgcccat cccgtcggca acaattttct tgttgtcggc accgaagaaa atcatatcgc
    27781 catcttgcgc ggcagtacga tccaggatgg cttcgatgat ttctgcatta aggaacttcg
    27841 ctaccgggct gttgatacct tccagacctt tcgcgcgttc gttaactttg atgtaagcca
    27901 gacctttcgc gccgtagatt ttaacgaagt taccgtattc gtcgatctgc ttacgggtca
    27961 gcgatgcgcc acccggaaca cgtagagcgg caacacggcc tttcggatcg ttcgctggcc
    28021 ccgcaaatac tgcaaactca acagatttca gcagatcggc aacgtcggtc agttccatcg
    28081 ggttacgcag atccggttta tcagaaccat aacggcgttc tgcttccgca aaggtcatta
    28141 ccgggaaatc gcccagatcc acgcccttca cttccagcca caaatgacgc accagcgctt
    28201 ccatcacttc acgcacttgc ggcgcggtca tgaaagaagt ttccacatcg atctgagtaa
    28261 attcaggctg acgatcagcg cgcaggtctt cgtcacggaa gcatttaacg atctgatagt
    28321 aacggtcaaa accggacatc atcagcagct gtttgaacaa ctgtggggat tgcggcagtg
    28381 cgtagaattt acctttgtgc acacgagaag gcaccaggta gtcacgcgcg ccttccggcg
    28441 tggctttggt cagcatcgga gtttcgatgt cgaggaagcc gtggtcatcc ataaaacggc
    28501 gcaccaggct ggtgatttta gcgcgggttt tcaggcgttg cgccatttcc gggcgacgca
    28561 ggtcgaggta gcggtatttc agacgcgctt cttcggtgtt gacgtggtta gagtcaagcg
    28621 gcagaacatc tgcgcggttg atgatagtca gcgaggacgc cagcacttca atttcgccgg
    28681 tcgccatatc gcggttaata tttttttcgt cacgcgcacg tacggtgccc gtaacctgaa
    28741 tgcagaactc attacgcagt tcagaggcca gctttaacgc gtccgcacga tccggatcga
    28801 aaaatacctg cacgatacct tcgcggtcgc gcatatcgat gaagatcagg ctaccaagat
    28861 cacgacgacg gttgacccaa ccacacagag tcacctgctg ccccacgtgg gacaaacgga
    28921 gctgtccaca atattctgta cgcatgagat atcccttaac ttagctgccg gcggatgccc
    28981 cctgctgcgc aggtgaccaa gtcgcagcgt tagctgtatg tcacaactga atgaaaaaag
    29041 gcggctatta tactggaaat tctgccgcac cgtaagagcc tggcccgcgc tggaacgcct
    29101 cgttaccact ttatatcggg cctgaaatca gactctacgc cagtttgcta taaaggtgtt
    29161 gcccgaactc ataaaaatta acaaaatttg tcgttccgcc atcggctaat cgcattaagg
    29221 tgtgaggcac gattttgttt tttcaggagt cagcatgctt gaacttaatg ctaaaaccac
    29281 tgcgttggtg gtgatcgatt tacaagaagg catcttacct tttgccggtg gtccacatac
    29341 tgccgatgag gtagttaatc gcgccgggaa gctggcggcg aaatttcgcg ccagcggtca
    29401 gcccgtgttt ctggtgcgag ttggctggtc tgccgattac gccgaagcat taaaacagcc
    29461 ggttgat
//
LOCUS       DAJTSA010000044        29089 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000044 DAJTSA010000000
VERSION     DAJTSA010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.044"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(397..1326)
                     /gene="virB"
                     /locus_tag="OO369_002102"
     CDS             complement(397..1326)
                     /gene="virB"
                     /locus_tag="OO369_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR5982374.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(1543..1779)
                     /locus_tag="OO369_002103"
     CDS             complement(1543..1779)
                     /locus_tag="OO369_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5982375.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            complement(1783..3684)
                     /gene="ipaA"
                     /locus_tag="OO369_002104"
     CDS             complement(1783..3684)
                     /gene="ipaA"
                     /locus_tag="OO369_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR5982376.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(3693..4691)
                     /gene="ipaD"
                     /locus_tag="OO369_002105"
     CDS             complement(3693..4691)
                     /gene="ipaD"
                     /locus_tag="OO369_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR5982377.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(4742..5833)
                     /gene="ipaC"
                     /locus_tag="OO369_002106"
     CDS             complement(4742..5833)
                     /gene="ipaC"
                     /locus_tag="OO369_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR5982378.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            complement(5853..7595)
                     /gene="ipaB"
                     /locus_tag="OO369_002107"
     CDS             complement(5853..7595)
                     /gene="ipaB"
                     /locus_tag="OO369_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR5982379.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(7601..8068)
                     /gene="ipgC"
                     /locus_tag="OO369_002108"
     CDS             complement(7601..8068)
                     /gene="ipgC"
                     /locus_tag="OO369_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR5982380.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(8125..8751)
                     /gene="ipgB1"
                     /locus_tag="OO369_002109"
     CDS             complement(8125..8751)
                     /gene="ipgB1"
                     /locus_tag="OO369_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR5982381.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(8767..9156)
                     /gene="ipgA"
                     /locus_tag="OO369_002110"
     CDS             complement(8767..9156)
                     /gene="ipgA"
                     /locus_tag="OO369_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR5982382.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(9169..10653)
                     /gene="icsB"
                     /locus_tag="OO369_002111"
     CDS             complement(9169..10653)
                     /gene="icsB"
                     /locus_tag="OO369_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR5982383.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            10967..12583
                     /locus_tag="OO369_002112"
     CDS             10967..12583
                     /locus_tag="OO369_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR5982384.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            12593..12955
                     /gene="ipgE"
                     /locus_tag="OO369_002113"
     CDS             12593..12955
                     /gene="ipgE"
                     /locus_tag="OO369_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR5982385.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            12955..13413
                     /gene="ipgF"
                     /locus_tag="OO369_002114"
     CDS             12955..13413
                     /gene="ipgF"
                     /locus_tag="OO369_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR5982386.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            13422..14537
                     /gene="mxiG"
                     /locus_tag="OO369_002115"
     CDS             13422..14537
                     /gene="mxiG"
                     /locus_tag="OO369_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR5982387.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            14545..14796
                     /gene="mxiH"
                     /locus_tag="OO369_002116"
     CDS             14545..14796
                     /gene="mxiH"
                     /locus_tag="OO369_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCR5982388.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            14809..15102
                     /gene="mxiI"
                     /locus_tag="OO369_002117"
     CDS             14809..15102
                     /gene="mxiI"
                     /locus_tag="OO369_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR5982389.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            15108..15833
                     /gene="mxiJ"
                     /locus_tag="OO369_002118"
     CDS             15108..15833
                     /gene="mxiJ"
                     /locus_tag="OO369_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR5982390.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            15830..16357
                     /locus_tag="OO369_002119"
     CDS             15830..16357
                     /locus_tag="OO369_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR5982391.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            16299..16994
                     /gene="mxiN"
                     /locus_tag="OO369_002120"
     CDS             16299..16994
                     /gene="mxiN"
                     /locus_tag="OO369_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR5982392.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            16987..17394
                     /gene="mxiM"
                     /locus_tag="OO369_002121"
     CDS             16987..17394
                     /gene="mxiM"
                     /locus_tag="OO369_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR5982393.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            17378..17806
                     /gene="mxiM"
                     /locus_tag="OO369_002122"
     CDS             17378..17806
                     /gene="mxiM"
                     /locus_tag="OO369_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR5982394.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            17936..18568
                     /gene="mxiE"
                     /locus_tag="OO369_002123"
     CDS             17936..18568
                     /gene="mxiE"
                     /locus_tag="OO369_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR5982395.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            18555..20255
                     /gene="mxiD"
                     /locus_tag="OO369_002124"
     CDS             18555..20255
                     /gene="mxiD"
                     /locus_tag="OO369_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR5982396.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            20274..21341
                     /gene="mxiC"
                     /locus_tag="OO369_002125"
     CDS             20274..21341
                     /gene="mxiC"
                     /locus_tag="OO369_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR5982397.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            21354..23414
                     /gene="mxiA"
                     /locus_tag="OO369_002126"
                     /gene_synonym="virH"
     CDS             21354..23414
                     /gene="mxiA"
                     /locus_tag="OO369_002126"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR5982398.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            23427..23828
                     /gene="spa15"
                     /locus_tag="OO369_002127"
                     /gene_synonym="spaK"
     CDS             23427..23828
                     /gene="spa15"
                     /locus_tag="OO369_002127"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR5982399.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            23832..25124
                     /gene="spaL"
                     /locus_tag="OO369_002128"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             23832..25124
                     /gene="spaL"
                     /locus_tag="OO369_002128"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR5982400.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            25217..25555
                     /gene="spa13"
                     /locus_tag="OO369_002129"
                     /gene_synonym="spaM"
     CDS             25217..25555
                     /gene="spa13"
                     /locus_tag="OO369_002129"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR5982401.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            25542..26420
                     /gene="spa32"
                     /locus_tag="OO369_002130"
     CDS             25542..26420
                     /gene="spa32"
                     /locus_tag="OO369_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR5982402.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            26414..27295
                     /gene="spa33"
                     /locus_tag="OO369_002131"
                     /gene_synonym="spaO"
     CDS             26414..27295
                     /gene="spa33"
                     /locus_tag="OO369_002131"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR5982403.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            27300..27950
                     /gene="spa24"
                     /locus_tag="OO369_002132"
                     /gene_synonym="spaP"
     CDS             27300..27950
                     /gene="spa24"
                     /locus_tag="OO369_002132"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR5982404.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            27965..28225
                     /gene="spaQ"
                     /locus_tag="OO369_002133"
     CDS             27965..28225
                     /gene="spaQ"
                     /locus_tag="OO369_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR5982405.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            28225..28995
                     /gene="spa29"
                     /locus_tag="OO369_002134"
                     /gene_synonym="spaR"
     CDS             28225..28995
                     /gene="spa29"
                     /locus_tag="OO369_002134"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR5982406.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            29002..>29089
                     /locus_tag="OO369_002135"
     CDS             29002..>29089
                     /locus_tag="OO369_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HCR5982407.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLT"
BASE COUNT         9090 a         4221 c         5747 g        10031 t
ORIGIN      
        1 gtgaacgaag tctgttaatg gtggattttt tgtttatcac catgtaaaaa aaattgagta
       61 gtaaaagcgt tgactgctaa tcctcgctgt ttttattgaa acgattaaaa atacgctgaa
      121 tattttcggc cagtcactca tattcacata aaattttgac catcatgccg catcccttag
      181 ttgcacaatt cattttcaat gatatgaata ttactaattt caccagataa ttttctcgtg
      241 gtcagcaggg tatgagtttt caccgtaatt ttaaggaaac acagtgcgtg gggattgaat
      301 aacagatagc cgacggaatt accccaccgg ctgaaagtca agttcaacga agatttttat
      361 tatctgaatt gggcagttta catcagtgtt cgatgtttat gaagacgata gatggcgaga
      421 aattatatcc cgaatagctt catctatttc tctctgaact gttttgttta ttttagataa
      481 tgtaaatatt agagttctgt ttgttttatt ctcttttctt tttatgtaag tccgtttgtc
      541 tttagaaata aataacgagt caacttttag cgaagggttt tgctttctgt ttttgctttt
      601 ctttatgatg tttaaaattt cttttttata tatgtccggg ggcaaatttg tatcaaggtc
      661 ttttatttct tcctttaata ttggtagtgt agaactaaga gattcattag ccttttcaag
      721 tcctttataa taattgaata atatcttgta gtcattaaag ttaagttctg acgcgattgg
      781 aaatagagat attatttcct gtggaacgct tgctgcctga aaggcacgag tgactttcgc
      841 gcgagacaga ttctcttttt tggctatgtc tttataggac atccctgata ctttcagaaa
      901 attaagacca ataccaagtt ctcggatgct atgctctttt gctgtttgta tatcgtttgc
      961 tagttttctg gcatcaagag tagatatata ttcttttgaa tatagaactt caagatctgc
     1021 tcctgcatat attgcagatg ctcttctacg agtgccatcc agaatttcaa ttctaccatc
     1081 aatctccctt cctattacag ggaagaattg ttgtagcttt atagttttta tgatatcggc
     1141 taatgattct tctgttaggg attcttgatc gcgtacattc gttttttggt tgacgaaggt
     1201 taaatcttgg atccttttat gaggaatctt ggctttgata aaggaaactt tattaccaga
     1261 atggagtgta aactctttct tttggccttc ctttatactt aacaagtcgt tgcacaaatc
     1321 caccatatca caccctgttt attcatattg atttatattg acaaaagtta aatgcagtgt
     1381 gagcaagatt gtatgtaact gttgatgata gtcaacggta gtcttaaaaa ttactatttt
     1441 aatagctgga ttgatgcaat ttatctttat tggataaaag aataatattc ttgtggagct
     1501 ctgatgtgag agctccatag atgaaaaaat gcttatcaat tactatattg attttgtact
     1561 atttttgact acagaaataa gatctccaat agtaataatg tgttctaatg tagattcatc
     1621 tatacgtaga ttgaactcaa aactaatcat atccagcatt tcaatcagag aaagagaatc
     1681 ggcagaaaga tcttcaataa atttagtttc ttcagatagt ttgctgtacg ctataccata
     1741 gcaaaaggcc actattgata atattttttc ttttatcata ttttaatcct tattgatatt
     1801 ctttaatact tttgataggg aagtggttac atcttttgct gctttatata tattattgtt
     1861 ttcagctgta atatcattgt tattttttaa atcagatata tcatctgtaa gtaattctgc
     1921 agaggtatcg tcaatttttg acaatacctt gctgagggcg ctagatactt cttttgcctt
     1981 ttcatagatc gcatgatttt tatcaattgt atctgcgttg gtttctgtta cgtagtttct
     2041 tatatttgca tctgtaactt ttaatttgtt gtttattcta tgttctgttg tggattttgt
     2101 aatgtccttt gatttttcct gtaactctcg ttcttgtgtt cctgatttag ttccaatgag
     2161 agatataaga ttacttaggg agtttgttac tttttttgaa gcttcaaata tcgtttcccg
     2221 tgttaaatat gtgtctccat ttcttgataa aaaatcagtc tgaagtactt ttgaatcagt
     2281 gtttgagttg aatttattga tttgcgaagc tactctatga gaaagagaag cgctactttt
     2341 agttgtatct gatgagttat cattactgct tgtcactcct tccgggtgga gagggggggt
     2401 ggttgaagag ttctgtatgt tcagaggcct gtgtccccga gaaagagagt tatttgcaga
     2461 tctttcttga ctactcgtag ttaacaggtc tgaacctatt ccatccgaat tatctggagt
     2521 atggttgaaa gccttttcta aagctgaatt atttgcaaat gtctggacag tatctgatat
     2581 agagtgtggt agaacggaag ttactttttg aactagtgct ctttgtggat ttagtaatga
     2641 gtttccgtta cgggataata gatcgtttgt ctgagaagta ggagactgtg catcattttc
     2701 tgggttctcg tgatagctat tgtcatatac tctgttgtca aaaactctat tatcgtaagt
     2761 tctattatca ttatttatat gatagtgaat tacaggttgg gatgttttct cattggctcc
     2821 gggggttggt ccgccatctg gcatatcttc gggaggtgta gggaggccat cttggatagg
     2881 atgcgaatct tttatatctt ctaaaatatc attaaggact tttctcaaca gctcaggaaa
     2941 cacggctact gtatttaagt ctcttgacag tttacctata ccaaatcctt tactatcaat
     3001 agtattttta cgttgattgc gcaaactatc aaaggcaagt tttgcctgtg cgtcaactaa
     3061 atcatctagt ttgagagatg aaacagtttc attacttaac tttaattcag caagaatatt
     3121 attcttgata aatgatgata cttcagtatt gaggagtttt tgtcccaatg ataaagcttc
     3181 accagatgtg tgttgttgtg ctaatagtgc aagtctgtgc tcaatgtttt gtcgtaattg
     3241 atggagtttc ttattctttg ccgaccatgg tccaaaggca gaatcttggg ataatttatc
     3301 ttttataatt agttccgaat actgtgcgac aattttttga agtccctttt cgttttcaaa
     3361 attaagacca ttttgatgta ctaaggagta caaaacattt gaaaattcga gaatctcgct
     3421 tttggcatac gcattcattg agttggaata aatgcttctt aacacatctt ctttccctga
     3481 ggaagaaaat aaaaaatcaa gacatttctg attaaaagac gttgcaaagt tttctttttc
     3541 agacactgat gctggtgttt ttagagaagt ttgcgaacta tggatatcgg atattttgct
     3601 ttttaactcg ctgtattctg ttgatgatgg tgaagttgcc ttatataaga atgttggcgc
     3661 ttgagtatta tttacattat gcatggcgca cctcagaaat ggagaaaaag tttatctgta
     3721 tctgtacatg agcttattgt actactcaaa acctttacta aattatcaaa aatactattg
     3781 gcattactgt atttttgaac taaagtttga agattatttt tcattgtttc atcttcggca
     3841 gagaatccgg cattccatgc ctgatatttt gcattatcta gcacaacctc gccatttcca
     3901 cctagattat ctaagctttt taacatattg tctattgggg tcatgtttat actgacaaca
     3961 tatcccccgt ttttttgaga taccttgccg attgttccac ctaattctgt aagccattta
     4021 tttgcttgtt cctgactaac agtattattt gctggatata gcggtttatc tttatatttt
     4081 tccttgagtt cttccaatgc ctttttaagc gagttgactt gtaatttcac ggagtttccg
     4141 tcgttacctc cgggagagat ccagccggca agactggaaa gaacagcgct aaaatcttga
     4201 tacatttgag tatatgaact aacggcatgt tcatatactt tcagatactg ttcattaata
     4261 tcattgatgg agtttgcaat tttagcccac agttctctat gagatatcat ttgatctcca
     4321 tcaagctcgg cttctttcgg agctgaatgt aataattctc ttgcgtcttt attaattgga
     4381 tattcgtgcc tggaaagaat atctaatagt tgagcggatt tatttacatc catgctaatc
     4441 tgagatgaat gtaatgctat ttcttctagc gatgttttag tcaacgtttt ttgtgaaagc
     4501 tctttcttta atgcctgatt tgttgttctg atattatgaa gggtgtcgtt gagcatagta
     4561 agggaactta caggatgaga actggtcgtt gtttttatat cagaattaac tgtttcggtt
     4621 gatgaaccgt tggtattgtt tggactgaat gatgaggtgg aaatactatt agtcagagtt
     4681 gttatattca tggttatttc cttacaggaa gagccatata ttattatggc tcttggatat
     4741 attaagctcg aatgttacca gcaatctgac tggctgtcga attctttgat tggttgatgc
     4801 tgtcaattat attcaataat ttttgtatta attgatttgt cgcttgggat gcttctttag
     4861 atacttggga tgcttcctct gcttgtttac tgctggcctg actgattagt tgttcttctt
     4921 cttcaagagc agatgcataa cgccctcctg atgtggatat attaccagca acggctgatg
     4981 tttccattgt tgcacggcca atgttttgtt tttgctgcgc agaaagggta ttgagctcgt
     5041 aagttcttct ctgggtgtca attttatcct gcaaagaaat atcgggagaa ctaagagaag
     5101 ttttatgttc agttgacagg gatatattat ctggcgccag tttattttta cctaaaaatt
     5161 ttgtgctaga gttagtttgt ggtgaggtga tatttgtatc tatttgttta tttaacccta
     5221 atttagaacc tgcaagctct ttttcaagag attgagcagt ggcaaggttc tttcttaagg
     5281 ctcctttttg gtcgctaatc cctgaatgcg tttttttggc accgataccc gttataccta
     5341 cttgtgtgac tgctccagta atgcttgatg atagggctgc caggccttgc cgaacaatgt
     5401 tctctgcagc tgattttgta gcatcgaacg caatcaatga caattgagag cccaatttag
     5461 tttctgcagt gcggagagca gaaagtagaa cggctacact aataattaaa gaaacagcat
     5521 tagaagaaag actggaaata tccagagtgt tctctggagt aagggtgtga ctcatctctg
     5581 aaagatcttt catctttttg tcagtaaggc gagcaatgat ttctgaatca tgctgaacct
     5641 gctctgataa ctttaaaagt tgatcatcat ttaatgtggt tgttaatacg ggatttttac
     5701 cgacattaag tggaatatgc gctgcaatct gctgataatt ttgtgatttt tgtgtttcgg
     5761 aagaactttg agtttgtttt gtggatatat ctgtatataa aatctgggtt ggttttgtgt
     5821 tttgaatttc cataacattc tccttatttg tatcaagcag tagtttgttg caaaattgct
     5881 tttgcaacat cagttctatt agcctgagag ttggacattg aggctaatag atctgcaatt
     5941 acttcctgca attggccgaa tttttctatt gcttcactga tatattttga cagttgttca
     6001 acttgatatt tcgataatgt caggtctgct agatttgtcg acgcgctgtt ctggaaaaca
     6061 gcagaagcga cacttcctcc cgcttgtgtg gcagagttaa cactttctcc taataaaact
     6121 gcttggttta aatgggtgga aataatttgt ttggatatta cttcatcacc cgctgcacca
     6181 agaaatttat ttaatctggc aacagcatta gtgattaaat cgtccagttg agaagaaaaa
     6241 ttcttgagaa actttggtat aaggtctgtg agggttttac ctattatttt gccaatattt
     6301 tctgcaagtt ttgctgctgc ctgtttacca acagtggcta cgagaacgac tgctgcaact
     6361 aggacaagag cgcctgcgat tgcccccaga atagagccaa tcattttggc ttttttcgag
     6421 tcgacgccca agccttcgag catttttgta aatgcatctg aaaggagttt gattaagggt
     6481 tcaatgactg ctttcatgat cggattcagg gcttgttcca tgaaggaatt gccggtcgct
     6541 gcttgtacta tagcatccgt aaccataaga gctaaaccaa cagctgccag tgctagagag
     6601 gctcctccag aaaaagctgc tgcaacaaca ctaacgatag ttaaaagtgc cccaagtatt
     6661 ttcccaacac aacccattac tctgttgagt tcttctgctt tacgtacttc agcagcatac
     6721 tcatcagatt ttctctccat ttcagttttt cttgattctt ggagagactg gaatagagcc
     6781 agatcatttt ttaaagattc ttcattattt tttccaacta gttgaataaa ggttgccatc
     6841 aattgagtaa cactggcaag tccggttaat gatttctgct gggttgatag ctgttcagca
     6901 gatgctgtgt ttgaaaatgc agaaaaagag tctatttctt tttcgagttg cattgattta
     6961 tctgtaagtt tgctatgaat tgacagggtt ttctgctcaa tcaatgtcct gtctttaact
     7021 gctgcgtctt ttttgatagt gagttgtatt tcttcccggc ttaatttttt cttttctggt
     7081 gactctgggt cgagttcgga taatcttgtt tgaatttggt taattttatt ttctaggtct
     7141 tttattttag aatctgcgtt ttttagttta ttaatttgtt tttcatagtc tctggttagt
     7201 ccttcagttt cagatagaag agtgttaatt ttatcggaga attctaggtt tttttgctgt
     7261 cttgcctgtt gctgggactt ccaagcagta attttatttg ttaatgcagt taaagatttt
     7321 tcaccgagta tttgaataag gtttccaatt aaaagcgtta gttgggaact tgcatttaat
     7381 gactttggtg ctttaagttc agggattaat atatttgaag ttgagtgtgc attagttgta
     7441 ttaatatttt ggttagcagt aagatcagca attgtaagag aaaataattt gttagctgca
     7501 tcatttgcag cttggatagt attgtctcca agctcagtgg aagccaatat tttggcaaga
     7561 ggaaaaccag tggttgtggt gcttacatta tgcataataa ttactccttg atatcctgaa
     7621 ttgcgtccaa gtatgattgt gcttttattt ttaatttttc atcattgctg tgttgaatta
     7681 cgagttcgaa gcactcttta gcttttaagg gggctttcaa ccgaagctgg cattgtccag
     7741 tatggaatac tggtgtatag tcattttttc ctaatgcaaa agcgacagca taaaggtctg
     7801 ctgcttgttg gaactgttct tttatctgat aaatagctgc gagtcccata atgtagtcta
     7861 cattgtaaaa gtcgtatata cataaaaacc tgaagaaaac ttcagcttcc tctattcttc
     7921 ctttgttgta aaagtcataa gcatatgaat aaatgtcatc catcatatca tcaggaattg
     7981 cattaatatc tttcagtgta gcgccagagt taattgcatc aattactgca gtagagatgc
     8041 tttcattttc ggtgatattt aaagacataa ggtctccttt ttgtctgctt tactttaata
     8101 agtataagat ttaatataaa agatttaatt tgtattgctt tgacggtata cagcctttag
     8161 aacctctgcg cttatttcta tttgtaaaac ttttgttgat tcattaaata ttttatcgta
     8221 gacggaactg gatactagat ttgtaagatc cttcatttgt gtgttaaaca aatccatgtc
     8281 actgctatag cgttttaaaa tctttgctct gaggccagat gaggcaatct cgtttagtgc
     8341 tgtatgactg atagatgttt gtgctgattt ttcatcaagt tttatatccg ggaatttttt
     8401 ttcaatagct gcaaatatgt tttttttgtt atctgaggtc aaaacaactc cattgtgtat
     8461 ggccatttca tctatacttc tgtttatcat tgaagagaca taagtggttc gctcttggct
     8521 catccaacaa aatagttgat tccccgaatt actatctttt attttgtcta tgacgctgtc
     8581 cctgatttta tttatattca ccgggcatcc aaaattcttc tgaggaaagc gttgttttaa
     8641 gttaaaaaca gataatattt tctttacaag cttattgcga cttaacgaat caaatgatgg
     8701 gcgaggaatc gcgaattcaa cctgtggaag tattttgttt agaatttgca tgataccccc
     8761 tatatgttag ttcacttctg aagtgatgtt tgcaatggtt agtttaatac aaaataaaaa
     8821 ttcatacaaa gattcaatga gttcatcagg tgaaagatat tttttgtagt gtttcttggt
     8881 caaaacacat tgatgagtat cagggagaag acagatagaa gaaccataat ctctactcaa
     8941 agagtagtta aggatgagta tgtgttctgg attaataata ttttctgtaa aaagttcatg
     9001 ttcattaaga agtccggtca aaaagatgta ttcatcatta tctgttatac aaacaggtat
     9061 ttgttcttcg agcaatataa aggcttgatt tttatcgtta aaatcaagct ttgcacctgt
     9121 tatttcataa agtttatcat atagtttgcg acacatttct atggccttct atatattaga
     9181 atgagagtta ttcaataaaa gcttattgat ctgtaataag tcgtgaatgt aaagtttggt
     9241 tccaagatct ggcgatttaa gagaattgta ataatcaata atttttattg atttttcgat
     9301 gatttcttct tttttatctg ttgcttcggt ttttaattcg tttaatacac gagtcatatt
     9361 gctgggtgct gattttacta agtatgagtg gcaagcgttg atgaccgcat caaagtcttc
     9421 tgaagataca ttttcagtag gatgtttgta aagtgaaatt gataaaggct tgaatgtttt
     9481 tggtgtgtgg ataaggttaa ttttattgaa acttttaaga tagctgcgtc gtaattgagt
     9541 caatctggaa agatcaaaat cagattcaat atacttcatt atttcgttat aagactgatt
     9601 gagactttca atgcgttgcc taactttatt tgcatacgcg tatacatcgt taggtgtagc
     9661 aaccaattta acgtcaggga gtggaaagta tatttcagaa tttcctgctt taagaacatt
     9721 aagtgccata ccagcacagt tttcagattt actgattaat ttataatttg ccaattgtct
     9781 gaaagcatct gctttctttt tgcatataaa ttttgacatg tttgtttcat caaggccaaa
     9841 aaggttatgg ctgattttac catcctttgt tttattatca cctgataatg gaatgtatac
     9901 tttatcagca ctgacccccc aataaccata ggggtctttt ttttgattag ctctgggaaa
     9961 aaatattttt ttgttgttat tttgatcttg ttttgaattt aataagtttc taacctcttg
    10021 gcctgcatca agtctttcgg ctgtttggtc tgaaatcata tttagtttgt cttgcttata
    10081 ggtatcgaca ctgtaacctg agcgtttttc aaaaaggtag tttatttctg tatcttttgt
    10141 aacattcccg ccgggatacc agctgagata ggtatgagtt ggttcctgta gtaaattgtt
    10201 ttttatactg atactagtat gccctaaatg ggttatgcct ctatttcccc agcaacgaaa
    10261 aactaaatgt ctttcaagat ttttggctgt accgatgcca tgaaaacgct ctgcggtatt
    10321 caacattaat ttgatgtctc tacctgtgaa agagtgttga attgagtttt tagctgcttc
    10381 ataggcggca aacgcacttt ctgcagattt ttcgctagct aatgtattga ggaaaagcaa
    10441 taaggatttt tgattctcta gtcgataatt ttttagttgt tcatttaata tctgtgaatt
    10501 tatctttgca ttaatagtgc ttaatgttct gaaaaagaga tcctttaggt tgaatttctg
    10561 tgctatcaat ataggtccat gctctgtatc ttcaacgcgt ataggccctt ttgtattgct
    10621 tgcgtcaatg aaattgctaa ttttgaggat catactttat taactctcca ttacttggtg
    10681 attgtgggga tgtaaaatgt atttggtata taatatattc aattaataaa tttagaaact
    10741 tgagcctgtt aacataatca aattttcttt tgctgtacat aatatgtacc tcgtgagcat
    10801 atgtagtgct cgtttcatca tgaaatccca caagataaag tgcctgatgt atcaggctcg
    10861 gagtgttata gaaaaagaga gaaccctgtt gaataagtat cttttttgtt gtaaaacgag
    10921 gtacaaaact gcaatcaatg ccccatttat gactgaggat aattaaatgc acataactaa
    10981 tttgggattg catcaggttt catttcaaag cggagattcc tataaaggcg cagaagaaac
    11041 cggaaagcac aaaggtgtaa gcgtgatttc atatcaaaga gttaaaaatg gagaaagaaa
    11101 taaggggatc gaagcgctta atcgattata tttacagaac caaacaagct taacaggaaa
    11161 aagccttttg tttgcgcgtg acagagctga agttttttgc gaggcaataa agttggcagg
    11221 tggtgatacg tcaaaaatta aagccatgat ggaacgatta gatacctata aacttggtga
    11281 agttaataaa agacatatta atgagcttaa taaagtaata agtgaagaga taagagcaca
    11341 gctaggcatt aaaaataaaa aggaattgca aacgaaaata aaacaaatat ttactgatta
    11401 tttaaataat aagaattggg gaccggtcga caagaatatt agtcatcatg ggaaaaatta
    11461 tggttttcaa ttaactcctg cctctcatat gaagataggg aataaaaata tatttgtcaa
    11521 agagtataat ggaaaaggaa tttgttgtgc ttctacgaga gagagtgatc atatcgcgaa
    11581 tatgtggctt tcaaaagtgg tggatgatga ggggaaggaa attttttcgg gtatcagaca
    11641 tggagttata tcagcctatg gattgaaaaa aaactcatct gaaagagctg ttgctgctcg
    11701 taataaagct gaggagttag taagcgcagc attatatagc agaccagagt tattatcaca
    11761 ggctttgtct ggtaaaacag tagatttaaa gattgtttcg acttctcttc tgacgccgac
    11821 cagtttaacc gggggagagg agagtatgtt aaaagatcag gtgaacgcat taaaagggtt
    11881 aaactctaaa agggggaagc caacgaaatt attaattcgg aatagtgatg gtcttctgaa
    11941 agaagtaagc gttaatctaa aagtggtgac atttaacttt ggtgtaaatg aattagcgct
    12001 aaaaatgggc ttaggctgga ggaatgttga caagcttaat gatgaatcaa tatgttcttt
    12061 gttgggggat aatttcctca aaaatggtgt gattggaggt tgggctgcag aagcaataga
    12121 aaaaaatcca ccatgtaaga atgatgtgat atatctggct aaccagataa aagagattgt
    12181 aaccaaaaag ttacaaaaaa atgataatgg agagccgtat aaattgtcac aaagaatgac
    12241 tctattggct tatactatag gtgctgtacc ttgctggaat tgtaagagtg ggaaggacag
    12301 aacaggtatg caggatgctg aaattaaaag agaaataata agaaaacatg aaacaggtca
    12361 gttttctcaa ttaaatagta aattatcctc agaagagaaa agattatttt ctactattct
    12421 aatgaatagt ggtaatatgg aaatccaaga gatgaatact ggtgtgcccg gaaataaagt
    12481 catgaaaaaa ttgccgctat cttcgttaga gctatcttat tctgaaagaa taggggaccc
    12541 aaaaatatgg aatatggtga aagggtattc gtcatttgta taagaggaat atatggaaga
    12601 tttagcagat gttatttgcc gagccttggg tatcccttta attgatatcg atgatcaagc
    12661 aatcatgctt gatgatgatg tgctaatata tattgaaaaa gaaggagatt caataaattt
    12721 attatgccct ttttgtgcat tgcctgaaaa tataaatgat cttatatatg cgttgagcct
    12781 aaattactca gaaaagatat gcttagctac tgatgatgaa ggcggaaatc taattgcgcg
    12841 attagatctt accgggataa atgaattcga ggatgtatat gttaatacag aatattatat
    12901 ttcgcgtgtg cgttggctga aggatgaatt tgcgcgaaga atgaaggggt attaatgtcc
    12961 cgtttcgtat ttattttgct gtgttttatc ccatatttag ggagagctga ttgttgggat
    13021 aaggctggtg aaaggtacaa tattccatca agtttgttaa aagcgattgc ggaaaaagaa
    13081 tccggattta ataaatctgc agtcaatgtt aataacaatg gaagtaaaga ttatggtata
    13141 atgcaaataa atgactttca ttctaaaaga cttagagaaa tgggatattc tgaggaaatg
    13201 ctaattagtc atccatgtct ttctgtacat tatgcagcaa agttattaaa tgagtttatg
    13261 atgatgtatg gaagagggtg ggaggctgtt ggagcctata atgcaggaac gtctccgaaa
    13321 aagaaaaaag aaagattaaa gtacgcagag gatatctata gacgctattt gcggatagca
    13381 gcagagagca agcagaataa tcgaaggata taagaggatt tatgtctgag gcaaagaact
    13441 caaatcttgc accattcagg ttattagtga aactcacaaa cggtgtcggg gatgagtttc
    13501 cgttatatta tgggaataac ttaattgttc tcggacggac gatagaaaca cttgagtttg
    13561 gaaacgacaa tttcccagaa aatattattc ctgtaacaga tagtaaatcg gatggtataa
    13621 tttatttgac cataagcaaa gataatatct gtcaatttag tgatgaaaaa ggtgagcaaa
    13681 ttgatatcaa ttcacaattt aattcatttg aatatgacgg gatttctttt caccttaaaa
    13741 atatgaggga ggataaatcg agaggccata tactaaacgg aatgtataag aaccactcgg
    13801 tgtttttttt ctttgctgtt attgttgtgt taattataat tttttccctc tctttaaaga
    13861 aagatgaagt aaaagaaatt gctgaaatta tagatgataa gagatatgga atagtcaaca
    13921 ctggacagtg caattatatt cttgccgaaa cacaaaatga tgcggtatgg gcgtctgtag
    13981 ccttaaataa aaccggattt accaaatgca gatatattct ggtgtcaaac aaagaaataa
    14041 atagaattca acaatatatc aatcaacgat tcccatttat caatttgtat gtgttgaatt
    14101 tagtgagtga taaagcggaa ttactggtgt ttttaagtaa agaaagaaat agttcaaaag
    14161 ataccgaact tgataaattg aaaaatgctc tgattgttga atttccgtat atcaaaaata
    14221 ttaaattcaa ctatcttagc gatcataatg caagggggga tgctaaaggt attttcacaa
    14281 aggtaaatgt ccagtataaa gagatttgtg aaaataataa agttacatat tctgtcagag
    14341 aagaactgac agatgaaaag cttgagctca ttaatagact gatttctgaa cataaaaata
    14401 tatatggaga tcagtatatt gagttttctg tacttttgat agatgatgat tttaaaggta
    14461 aatcatatct taacagcaaa gacagttatg taatgttgaa tgataaacac tggttttttt
    14521 tagataaaaa taagtgagga taaaatgagt gttacagtac cgaatgatga ttggacattg
    14581 agttcattat ctgaaacttt tgatgatgga actcaaacat tacaaggtga actaacattg
    14641 gcactagata aattagctaa aaatccttcg aatccacagt tgctggctga ataccaaagt
    14701 aaattatctg aatatacatt atataggaac gcgcaatcca atacagtgaa agtgattaag
    14761 gatgttgatg ctgcaattat tcaaaacttc agataatagg gagcattcat gaattacatt
    14821 tatccagtca atcaggttga tattatcaaa gccagtgatt ttcaatctca agagatatca
    14881 agtctggaag acgtcgtgtc ggctaaatat agtgatatta agatggatac agatattcaa
    14941 gtatcacaaa taatggagat ggtaagcaat ccagaatcat taaacccaga atctttggcc
    15001 aagttacaga cgacgctctc aaattattca ataggagtat cattagctgg cacgttagca
    15061 agaaaaacag tttcggctgt tgaaacttta ttaaagtctt aatttatatg attaggtata
    15121 aaggttttat tttattcttg ttgctgatgt tgattggatg tgagcaacgt gaagagttaa
    15181 tttctaattt atctcaaaga caggcaaatg aaataatatc tgtgctagaa cgccataata
    15241 ttactgctag aaaagttgat ggaggtaaac aggggatctc ggtacaagtc gaaaagggga
    15301 catttgcatc ggcagttgat ttgatgcgca tgtacgattt gccaaatccg gagagagttg
    15361 atatctcaca aatgtttcct acagattcat tagtgtcttc tccaagagct gaaaaggccc
    15421 gtttatatag tgctattgag caacggctgg aacagtcttt agtttctatt ggtggtgtta
    15481 tttcggcaaa aatacatgtt agctatgatc ttgaagaaaa aaatatatct tcaaaaccga
    15541 tgcatatatc agtaatcgct atatatgact caccgaaaga gtctgaacta ttagttagta
    15601 atattaagcg atttttgaaa aacacctttt ctgatgttaa gtatgaaaat atatctgtca
    15661 tattaactcc gaaagaagaa tatgtttata caaatgtaca acctgttaag gaagttaaat
    15721 cggaattttt aacaaatgaa gtaatatatt tatttctcgg gatggctgta ctagttgtca
    15781 ttcttttggt atgggcattc aaaacagggt ggttcaagag aaacaaaata tgataagaat
    15841 ggatggaatt tataaaaaat atctttcaat aatttttgat ccagcgttct atataaatag
    15901 aaatcggttg aatttgcctt ctgaactgtt agaaaatggc gtaatcagaa gtgagattaa
    15961 taatctcata attaataaat atgatctaaa ttgcgatatt gaacctttaa gcggggtaac
    16021 cgctatgttt gttgccaact ggaatttact tccagctgtt gcttatttta tcggtagtca
    16081 agagtctcgg ttaattaatc atagtgagat ggttattagt tattatgggg gcaaaatcag
    16141 taagcaaggg gaggccgcta ttcgaagtgg tttttggcat cttattgcgt ggaaggaaaa
    16201 tatatctgtt ggtatatatg aaagaataaa cttattattt aatccaattg ctttggaggg
    16261 aaattatacg cctgtagaaa gaaatttgtc ccgattaaat gaaggtatgc aatatgcaaa
    16321 aaggcacttt accggtatcc agacatcatg cctatgatgg tgttgttata aagagaattg
    16381 aaaaggaatt atgtaaaacc ataaaagata gagacactga gagtaagaag aaagcaattt
    16441 gtgttataaa ggaggctacg aaaaaagctg aatcactgcg tatagatgct gtatgtgatg
    16501 gttatcaaat tggtatacag actgcttttg agcatattat tgactatatt tgtgaatgga
    16561 agttaaagca aaatgaaaat agaagaaata ttgaggacta tataactagt ttgctttcag
    16621 aaaatttaca tgatgagagg atcattagca ctttattgga gcagtggctt agttcgttgc
    16681 gtaatacagt aacagaatta aaagtggttt tgcccaagtg taatttagca ctgaggaaaa
    16741 agttagaact tgatctccat aaatatcgct cagatgtcaa gattatttta aaatattctg
    16801 aagggaataa ttatatattc tgtagtggta atcaagtagt ggagtttagt cctcaggatg
    16861 taatatcagg tgttaaaata gaacttgcgg aaaaattaac aaaaaatgat aaaaaatatt
    16921 tcaaagagtt agcacataag aaactgagac aaattgcaga agacttgtta aaagagaatc
    16981 ctgttaatga ttaatcaaat aaatgcaagc aatgctctgc agcaaagatt aaatagtgaa
    17041 gaagttgtta atttaaatga gagattatca tcttcgcaat catttgatga ggatataatc
    17101 tatgaaataa tgcaatattt ctcacagagt gaattaaatt ccatagataa tgatgaactg
    17161 cacaataaga ttgaacaatt attcaattca cgttttcctt atcttacagc tgcacaaaaa
    17221 agttcacttc ttaataaact catagatgca aatcaatatg tcgatttgca tgagggattt
    17281 tatgcatcat tgtccatata taacaatatt gatttctata ttaagactac aaccttcgat
    17341 tcattaatta gtgtctttga agcagggaga gaggcagatg attcgacatg gtagtaataa
    17401 gttgaaaata tttattttaa gtatattgct attaacactg agtgggtgtg ctttaaagtc
    17461 atcatctaat tctgaaaaag aatggcatat tgttcctgta agtaaggatt atttttctat
    17521 tccaaatgat ttattatggt cgtttaatac aaccaataaa agtataaatg tttactctaa
    17581 atgtattagt ggtaaggcgg tttatagttt taatgcaggt aaattcatgg gcaactttaa
    17641 tgttaaggaa gtagatgggt gcttcatgga tgcacaaaag atagctatag ataaactatt
    17701 ttctatgctg aaagacgggg ttgttttaaa aggtaataag ataaatgata ccatccttat
    17761 agagaaggat ggggaagtta aattaaaatt aattcgaggg atataattgt attgtgagta
    17821 aatataaagg tctaaataca agtaatatgt tttacattta ctctagtgga catgaaccag
    17881 ttaacgttga gcttgtaaaa gataaagaac gtaacataat tgagctggct ccagcatgga
    17941 agggtttttt tttgtccgaa atcaaaatat aaaattcagt gataatgtga actatcatta
    18001 tcgctttaac attaatagtt gtgcaaaatt tcttgctttt tgggactatt tcagtggtgc
    18061 tctagtagag catagccatg ctgaaaaatg tattcatttc tatcatgaga atgatcttcg
    18121 ggatagttgt aataccgaat cgatgcttga taagttaatg ttaagattca tcttttcatc
    18181 tgaccagaat gtaagtaatg cactggctat gatacgaatg actgagtcat atcatttggt
    18241 tctttatctt ctgagaacta ttgagaaaga aaaggaggta agaataaaga gtctgacaga
    18301 gcattatggt gtgtcggagg cttattttag atctttatgt agaaaagctt taggggcaaa
    18361 agtcaaagaa caactgaaca cttggcggtt agtaaatggt ttattagatg tatttttgca
    18421 caatcaaaca atcacttcag ctgccatgaa taatgggtat gcatcaacat cccatttttc
    18481 aaatgagata aaaacacgac taggcttttc tgcaagagaa ttatcgaata ttactttttt
    18541 agtgaaaaaa ataaatgaaa aaatttaata ttaaatcttt gactctcttg attgtattgt
    18601 taccattgat agttaatgct aataatattg actctcattt attagaacaa aatgacatag
    18661 cgaaatatgt tgctcaaagt gatacggtag gatctttttt tgaacgtttt tctgcgttat
    18721 taaattatcc tattgttgtt agcaaacaag ctgcaaaaaa gagaatatct ggggagtttg
    18781 atttatctaa tccagaagaa atgcttgaaa aactgacatt acttgtggga ctcatatggt
    18841 ataaagatgg aaatgcatta tacatatatg actctggaga attaataagc aaagttattt
    18901 tgctggagaa tatatcactt aattatctta ttcagtatct gaaggatgct aacctttatg
    18961 atcacagata tcctataaga ggaaacatta gtgataaaac attctatata tctggtcctc
    19021 cggcattggt tgaactggta gctaatacag caactttgct tgataagcag gtttcttcta
    19081 ttgggacaga taaagttaat tttggggtta taaaattaaa aaatacattt gttagtgaca
    19141 gaacttataa tatgagaggg gaggatattg tcatcccagg ggtggctact gttgtagaac
    19201 gtttattaaa taatggtaag gctctgagta acagacaagc ccaaaatgat ccgatgccac
    19261 cctttaatat tacacagaaa gtatctgagg atagtaatga cttttcattt agttcagtta
    19321 ccaatagttc tatattagag gatgtatcac ttattgcata tccagaaacc aattctatat
    19381 tagtcaaagg taatgatcag caaatacaga ttattcgcga tattatcact cagttagata
    19441 tagccaaacg acatatagaa ttatcattat ggataatcga tattgataaa tctgaattaa
    19501 ataaccttgg tgttaattgg caaggaacgg cttcatttgg tgattcgttt ggtgcgtcat
    19561 ttaatatgag ctcatcagca tcgataagca ctttggacgg caataagttt attgcttcag
    19621 ttatggcatt aaatcagaaa aagaaagcaa atgtagtttc aaggccggtg attttgacac
    19681 aagagaatat tccggctata tttgataata acaggacttt ttatgtgtca ttagttggtg
    19741 agcgcaattc ttcgttggag catgttacgt atggaacatt gataaatgtt atacctcgct
    19801 tttcatctcg gggacaaatt gagatgtctt taacgataga ggatggaaca ggaaatagtc
    19861 agtcaaatta caattataat aatgaaaata catctgtcct tccagaagtt ggaaggacca
    19921 aaatcagtac aatagcgaga gttccacaag gcaaaagttt gttgataggt ggttatacgc
    19981 atgaaacgaa ctctaacgaa ataatatcta ttccttttct ctcctctatt cctgtaatcg
    20041 gtaatgtgtt caagtataaa acaagtaata tcagtaatat tgtaagggtt tttctgattc
    20101 aaccccgtga gataaaggag tcaagttatt ataatactgc agaatataaa agcctcatat
    20161 ctgaaagaga aatacaaaag actacacaaa taatcccatc tgaaactact ttgctggaag
    20221 acgaaaaatc attggtttca tacttaaatt actaactata aagtaggtga tgtatgcttg
    20281 atgttaaaaa tacaggagtt tttagctctg cattcattga taagttgaat gcaatgacaa
    20341 attcagatga tggagatgag actgctgatg cagagcttga ttctggcttg gctaatagca
    20401 agtatattga ctcatctgat gagatggctt ccgctctttc gtcatttata aacagaagag
    20461 accttgagaa actgaaagga acaaatagtg atagtcagga acgtatttta gatggggaag
    20521 aagatgaaat taatcacaag atttttgatt taaagagaac gttaaaagat aaccttcctc
    20581 tagatcggga ttttatagac agactaaaga gatattttaa agatccaagt gatcaagtct
    20641 tagcattaag ggaacttttg aatgaaaaag atcttactgc tgaacaagtc gaattattaa
    20701 ctaaaattat taatgagata atatcaggta gtgaaaaaag tgttaatgct ggaataaatt
    20761 cagctataca ggctaaatta tttggcaaca aaatgaaact tgaaccacag cttttgcgtg
    20821 catgttatcg tggttttatc atggggaaca tatcaacaac agatcagtat atagaatggc
    20881 ttggtaattt tggttttaat cacagacata caattgtgaa ttttgtagag cagtcactga
    20941 ttgtagacat ggattctgag aaaccgagct gtaatgctta tgagtttggt tttgtgttat
    21001 ctaaattaat tgcaattaag atgattagaa cttcagacgt aatttttatg aagaaactgg
    21061 aatcctcaag cttgctaaaa gatggcagtt taagtgcaga gcagctattg ctaactttat
    21121 tatatatttt tcaatatcca agtgaaagtg agcaaattct tacttctgtt atagaagtat
    21181 cacgagccag tcatgaggat tctgtagtgt atcaaacata tctatcttct gttaatgaaa
    21241 gtcctcatga tatatttaaa agtgaaagtg aaagagaaat tgcgatcaat attctacgag
    21301 agcttgtcac aagtgcatac aagaaagagc tttctagata acaggagata aaagtgatcc
    21361 agtcttttct taagcaagta agtactaagc ctgaattaat tatattagtg cttatggtta
    21421 tgatcattgc tatgttgatc ataccattac ctacatacct tgttgatttt ttaatagggc
    21481 ttaatattgt acttgctatt ctcgttttta tgggatcatt ttatattgaa agaatattga
    21541 gtttttctac attcccttcg gttttgttga taacgacatt atttcgtctc gctttatcaa
    21601 taagtactag tcgactaatt ttagttgatg cggatgcggg taaaattatt actacatttg
    21661 gccagtttgt cattggtgat agtctggctg taggttttgt tattttttca atagtaactg
    21721 ttgtgcagtt cattgttata acaaaaggat ctgaaagggt tgcggaggta gctgctcgct
    21781 tttctcttga cggtatgccg gggaaacaaa tgagcattga tgcggattta aaggctggaa
    21841 ttattgatgc cgcaggagct aaagaaagac gcagtattct ggaacgtgag agtcaattgt
    21901 atggttcatt tgatggagca atgaagttca ttaaaggtga tgcaattgct ggcatcatta
    21961 ttatttttgt caatttaata ggaggtattt ctgttggtat gagtcaacat ggaatgtccc
    22021 tatctggtgc attatctact tataccatac taactattgg tgacggattg gtctctcaga
    22081 ttcctgctct gttaatttct ataagtgcag gatttatcgt tacaagagtt aatggtgata
    22141 gcgataatat gggacgtaac ataatgtccc agatatttgg gaatcctttt gttctcattg
    22201 ttacatcagc tcttgccttg gcgataggga tgttgccagg ttttccgttt tttgtttttt
    22261 tcctgatagc agttactttg acggctttat tctattataa aaaggtcgta gaaaaagaaa
    22321 aaagtctgtc tgagtctgat tctagtggct atactggtac atttgatatt gataatagcc
    22381 atgactcatc tttggcaatg atagaaaatc tggatgctat tagttcagaa accgttcctt
    22441 taattttatt atttgccgaa aataagataa atgcaaatga tatggaaggt cttattgaaa
    22501 ggataagaag tcagttcttt attgattatg gtgtcaggct tccaactatt ttatatagaa
    22561 caagcaatga gcttaaggtc gatgatattg ttttgttaat aaatgaggtg cgtgccgata
    22621 gttttaatat atattttgac aaagtgtgta ttacagatga aaatggagat atagatgctc
    22681 taggtattcc tgttgtttca acttcatata atgaacgtgt tatttcttgg gtagacgttt
    22741 catatacaga aaatctaact aatattgatg ctaaaattaa aagtgctcaa gatgaatttt
    22801 atcaccagtt gtcacaagct ttattaaaca acataaatga gatttttggt atacaagaaa
    22861 caaaaaatat gttagatcag tttgaaaatc ggtatcctga tctattaaag gaagtcttcc
    22921 gacatgtgac tatacagaga atttctgagg tattacaaag attgcttgga gaaaatattt
    22981 ctgttcgcaa tttaaaactt attatggagt ctttggcgct ttgggctccc agagaaaaag
    23041 atgtcataac attagttgaa catgtccgtg catcactttc taggtatatt tgtagtaaaa
    23101 tagctgtttc tggtgagatt aaagttgtga tgctttccgg atatattgag gatgcaataa
    23161 gaaaagggat aaggcaaacc tctggtggct ctttcttgaa tatggatata gaggtttcgg
    23221 atgaggtaat ggaaacttta gcacatgctt tgagagaatt gagaaatgca aaaaaaaatt
    23281 tcgttctttt ggtatcagta gatatacgta ggtttgttaa aagacttata gataacagat
    23341 ttaagagtat actcgttata tcgtatgctg agattgatga agcatatacc attaatgtat
    23401 taaagactat ttagtgaggt ttaaatatga gtaacattaa tttagttcaa ttagttagag
    23461 atagtctttt cacgattggt tgtcctccat caattattac agatttagac agccattcag
    23521 cgattaccat ttctttggat agtatgcctg ctattaatat agctcttgtc aatgagcagg
    23581 ttatgttatg ggctaatttt gatgcaccaa gtgatgtaaa acttcagtca tctgcttata
    23641 acatattaaa tttaatgtta atgaacttta gttatagcat taatgagctg gtagagcttc
    23701 atcgttctga tgagtactta cagttaagag ttgttattaa agatgactac gttcacgatg
    23761 ggattgtttt tgcagagatt ctgcatgagt tttatcagag gatggaaatc ttaaatgggg
    23821 tcttataatc aatgagctat acaaaattgc tcactcaatt atcttttcct aatagaatct
    23881 cggggccaat cttggaaaca agtcttagcg atgtttcgat tggtgagatt tgtaacattc
    23941 aggctggaat tgaaagtaat gaaattgttg caagagctca ggttgtagga tttcatgatg
    24001 aaaaaacaat attaagcttg attggaaatt ctcgtggact ttcacggcaa acgttgatta
    24061 agcccactgc ccagtttctt catacgcaag tcggccgtgg attattggga gcagtagtca
    24121 atcctttagg ggaggttact gataagtttg ctgttacaga taacagtgaa attctttatc
    24181 gacctgtaga taatgctcct ccgctatata gtgaaagggc tgcaattgag aagccttttt
    24241 taacaggtat taaggttatt gattctttac tcacgtgtgg tgaaggacag cgaatgggga
    24301 tttttgcgtc agctggttgt ggcaaaactt ttctcatgaa tatgctcatt gaacatagtg
    24361 gtgctgatat atatgttatt gggttaattg gtgagcgagg tcgagaggtt actgaaacgg
    24421 ttgattattt gaaaaactct gagaaaaaaa gcaggtgtgt tttagtatat gcaacttcgg
    24481 attactcttc ggttgatcgt tgtaatgctg catatatagc cactgctata gccgaatttt
    24541 ttaggactga aggacataaa gtagcgcttt ttattgattc attaacaagg tatgccagag
    24601 cattacgtga tgtggcctta gccgctggag aatcacctgc cagaagaggc tatccggttt
    24661 cggtttttga tagcttaccc agacttcttg aaaggccagg aaagttaaag gcaggtggct
    24721 ctattactgc attttacact gttcttttgg aggatgatga ttttgctgat ccattagctg
    24781 aagaggtaag atccatttta gatggacata tatatttgag cagaaatcta gcccaaaaag
    24841 gacaatttcc tgcaattgat tccttaaaaa gtataagcag ggtatttaca caggttgttg
    24901 atgaaaaaca tcgtattatg gccgctgcat ttcgggagct actttctgaa atagaagagc
    24961 taaggacaat tattgacttt ggtgaataca aaccggggga gaatgcctct caggataaaa
    25021 tctacaacaa aatatctgtt gttgagagtt ttctgaagca agattatcga ctcggtttta
    25081 cttatgagca gacaatggag cttattggtg aaacaattag ataaggtttt aaaaatcaaa
    25141 gataaatatc aacggtctgt taaattaatt gaagcacaca tattaacact aaaaaaaaaa
    25201 ctctttgtat cgagatgtgg aggcattaga taaaaggatt atatacttcc tgcaactgga
    25261 gaatgactta gaacctgtag gcgctcagag tgttagtcag cttttcaata caagacgtaa
    25321 gatcgctata gtaaaaaagc atattatcca atatcagtct gaacgaattc ttctaaaggg
    25381 aagaattgaa gaaattcaaa aagatattga tgaggcaaat gcttctaaaa gaaaactgct
    25441 tcataaagag agtaaaatat gtaaacggat tggcttgatt aaaagaaata actttgccaa
    25501 acagttgata cttgatgagt tatcacaaga ggatatgaag tatggcatta gataatataa
    25561 acctaaattt tagtagtgac aaacagatag aaaaatgtga gaaactatct tcaatagata
    25621 atattgatag tctcgttttg aagaagaaga ggaaggttga aattcctgag tactctttaa
    25681 ttgcatctaa ctattttact attgataagc actttgaaca taagcatgat aaaggagaaa
    25741 tttatagtgg cattaaaaat gcgttcgaac ttagaaacga acgagcgaca tattctgata
    25801 ttccagaatc aatggccatt aaagaaaata ttttgatacc agatcaagat atcaaagcaa
    25861 gggaaaaaat aaatatcggc gatatgaggg ggatcttttc atataataag agtggaaatg
    25921 cagacaagaa cttcgaaaga agtcatactt cttctgtaaa ccctgataat ctgctagaat
    25981 ctgataatag aaatggtcaa attggtttaa aaaatcatag cttgtctatt gataagaata
    26041 ttgctgacat catttcttta ctaaatggaa gtgttgctaa atcatttgag ctgcctgtaa
    26101 tgaataaaaa tactgcagac ataaccccat ccatgtcatt gcaagaaaaa tcaatagttg
    26161 aaaatgataa aaatgttttt caaaaaaata gtgaaatgac ttaccacttt aaacagtggg
    26221 gggctggaca ttctgttagt atttcagtgg agtctggttc ttttgttcta aaaccgtcag
    26281 atcaatttgt aggaaataaa cttgacttaa ttttgaaaca agatgctgag ggtaattaca
    26341 gatttgatag cagtcaacat aataagggga ataaaaataa tagtacagga tataatgaac
    26401 agagtgaaga agaatgctaa gaattaaaca ttttgacgct aacgaaaaac tacagatttt
    26461 atatgcaaag caactctgcg agcgtttttc gattcagaca ttcaaaaata aatttacagg
    26521 cagtgaaagt ttagtcactc ttacttccgt gtgtggggat tgggtaattc gtattgatac
    26581 attatctttt ttgaaaaaaa aatacgaggt attttcagga ttttctacac aagaatcttt
    26641 actgcattta tcaaaatgtg tctttataga gtcgtcatct gtattttcga ttccagaact
    26701 gtctgataag attactttcc ggatcacgaa tgaaatccag tatgcaacta ctgggagtca
    26761 tttatgctgt ttttcctctt ctttaggtat tatttatttt gacaagatgc cggtattacg
    26821 taatcaagtt tctcttgact cattgcatca tcttttagag ttttgcttag gttcatctaa
    26881 tgtaaggctg gctactttaa aaagaattcg cactggtgat ataatcatag ttcagaaact
    26941 ttataattta ttattgtgta atcaagttat tattggggat tatattgtga atgataataa
    27001 tgaggcaaaa attaatctgt cagaaagtaa tggtgagtca gaacacacag aagtttcttt
    27061 ggcattattc aattatgatg atatcaatgt aaaagtggac tttattcttt tagaaaaaaa
    27121 tatgacaatc aatgaactaa aaatgtatgt agaaaacgaa ttattcaagt ttcccgatga
    27181 catagttaaa catgtaaata ttaaagtaaa tggttctttg gttgggcatg gggaacttgt
    27241 ttctattgag gatggttatg gtatcgagat tagttcttgg atggtaaagg agtaatctca
    27301 tgctgagtga catgtccctc atcgctacac tttctttttt tacgctatta ccatttttag
    27361 ttgctgctgg tacctgttat ataaagtttt ctattgtttt tgtaatggta cgaaatgcac
    27421 ttggtttgca gcaggttcca tcaaatatga ctctcaatgg tattgcattg attatggctt
    27481 tgttcgtgat gaaacccatt attgaagctg gttatgaaaa ttatctaaat ggccctcaaa
    27541 aatttgatac aatatctgat attgtccgtt tttctgattc tggcttgatg gaatataagc
    27601 aatacttgaa aaagcataca gatcttgagt tagcacgttt tttccaaagg tctgaagaag
    27661 aaaatgctga tttaaagagt gcagagaata atgattattc attgttttcc ctcttgcctg
    27721 catatgcgct aagtgaaatc aaagatgcgt tcaaaatagg tttttattta tatttgccat
    27781 ttgttgttgt tgatcttgtt atttcaagca ttttacttgc acttggtatg atgatgatga
    27841 gtccaatcac aatatcagta ccaataaaac ttgtattatt tgttgcgctt gatgggtggg
    27901 gtatcctgtc taaggccttg attgaacaat atatcaatat tcctgcttag atgatggagg
    27961 taatgtgtct gatatagttt atatgggtaa taaggctctt tatttaatcc ttatcttttc
    28021 cttatggcca gtaggtatag ctacggttat tggattatct attggtttgt tacagacagt
    28081 gactcaactc caagagcaga cacttccttt tggtataaag cttataggtg tctcaatatc
    28141 tttgctactt ctttctggat ggtatggtga ggttttattg tctttttgtc atgaaataat
    28201 gtttttaatt aagagtgggg tttgatggac atttcaagct ggttcgaaag tattcatgtg
    28261 tttttaatac tcctgaacgg cgtttttttt agattggctc cattattttt ctttcttcca
    28321 tttttaaata acggtataat ttctccatct attagaatac ctgtgatttt tcttgttgca
    28381 tcaggattaa ttacttctgg taaggtagac ataggttctt ctgtttttga acatgtttat
    28441 ttccttatgt tcaaggaaat aattgttggc ctccttctct ctttttgctt gtctcttccc
    28501 ttttggatat ttcatgctgt tggtagcatt attgacaacc agcgtggggc aacgcttagt
    28561 agttcaattg atcctgccaa tggtgttgat acgtctgagt tggcaaaatt tttcaatctt
    28621 ttttctgcag ttgtatttct atacagtggt ggtatggtct ttattttaga atccatacaa
    28681 ttgtcttata atatatgccc gttattttct caatgttctt tccgtgtctc aaatatctta
    28741 acatttctga ctttattggc aagtcaggct gttattttag ccagtcctgt tatgatagta
    28801 ttgttactat cagaagtatt acttggtgta ttatcgagat ttgctccgca gatgaatgct
    28861 ttttccgtat cattaactat taaaagttta cttgcaatat ttattatttt catctgttct
    28921 tctactattt acttttctaa agttcaattt ttcctcggtg aacataaatt tttcacaaat
    28981 ctatttgtta gataaaatat tatggcaaat aaaacagaaa agccgacacc taaaaaacta
    29041 aaggatgccg caaaaaaagg acagtcattt aaatttaagg atttaacga
//
LOCUS       DAJTSA010000045        29043 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000045 DAJTSA010000000
VERSION     DAJTSA010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.045"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            109..1140
                     /gene="mdtN"
                     /locus_tag="OO369_002136"
     CDS             109..1140
                     /gene="mdtN"
                     /locus_tag="OO369_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR5982408.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1140..3182
                     /gene="mdtO"
                     /locus_tag="OO369_002137"
     CDS             1140..3182
                     /gene="mdtO"
                     /locus_tag="OO369_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR5982409.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            3179..4645
                     /gene="mdtP"
                     /locus_tag="OO369_002138"
     CDS             3179..4645
                     /gene="mdtP"
                     /locus_tag="OO369_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR5982410.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4843..5082
                     /locus_tag="OO369_002139"
     CDS             4843..5082
                     /locus_tag="OO369_002139"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5982411.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5092..5262
                     /locus_tag="OO369_002140"
     CDS             5092..5262
                     /locus_tag="OO369_002140"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5982412.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5311..6723
                     /gene="fdhF"
                     /locus_tag="OO369_002141"
     CDS             5311..6723
                     /gene="fdhF"
                     /locus_tag="OO369_002141"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR5982413.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFNAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6724..6990
                     /locus_tag="OO369_002142"
     CDS             6724..6990
                     /locus_tag="OO369_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HCR5982414.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7084..7773
                     /locus_tag="OO369_002143"
     CDS             7084..7773
                     /locus_tag="OO369_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5982415.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OO369_002144"
     CDS             complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OO369_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR5982416.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OO369_002145"
     CDS             complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OO369_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR5982417.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OO369_002146"
     CDS             complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OO369_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR5982418.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10475..12193)
                     /locus_tag="OO369_002147"
     CDS             complement(10475..12193)
                     /locus_tag="OO369_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR5982419.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OO369_002148"
     CDS             complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OO369_002148"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR5982420.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OO369_002149"
     CDS             complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OO369_002149"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR5982421.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OO369_002150"
     CDS             complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OO369_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR5982422.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OO369_002151"
     CDS             complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OO369_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR5982423.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16274..18232
                     /gene="acs"
                     /locus_tag="OO369_002152"
                     /pseudo
     CDS             16274..18232
                     /gene="acs"
                     /locus_tag="OO369_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            18432..18746
                     /locus_tag="OO369_002153"
     CDS             18432..18746
                     /locus_tag="OO369_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR5982424.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18743..20392
                     /gene="actP"
                     /locus_tag="OO369_002154"
     CDS             18743..20392
                     /gene="actP"
                     /locus_tag="OO369_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR5982425.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20570..21862
                     /locus_tag="OO369_002155"
     CDS             20570..21862
                     /locus_tag="OO369_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5982426.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22016..23665)
                     /locus_tag="OO369_002156"
     CDS             complement(22016..23665)
                     /locus_tag="OO369_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR5982427.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OO369_002157"
                     /gene_synonym="yjcD"
     CDS             complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OO369_002157"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR5982428.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="OO369_002158"
     CDS             complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="OO369_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR5982429.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26421..26744
                     /gene="soxS"
                     /locus_tag="OO369_002159"
     CDS             26421..26744
                     /gene="soxS"
                     /locus_tag="OO369_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR5982430.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="OO369_002160"
                     /pseudo
     CDS             complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="OO369_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28753..29034
                     /locus_tag="OO369_002161"
     CDS             28753..29034
                     /locus_tag="OO369_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR5982431.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7284 a         7777 c         7351 g         6631 t
ORIGIN      
        1 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
       61 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
      121 ccgaaaaaag cacctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt
      181 gcccttgttt tcgttatctg gcgcgtagac agtgcgccat caactaatga cgcttacgcg
      241 tcagcagata ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc
      301 accgacaacc aggcggtgaa acagggcgat ttgctgttcc gcatcgaccc gcgcccgtac
      361 gaagccaacc tggcgaaagc cgaagcctcc ctcgcggcgc tggataagca aattatgctc
      421 acccagcgta gcgttgacgc ccaacagttt ggtgccgact cggttaatgc cacggtagaa
      481 aaagcccgtg ccgccgcgaa acaggccaca gatacattac gccgcaccga gccattactg
      541 aaagagggtt ttgtctcagc agaagacgtt gaccgtgcaa gaacggcgca gcgcgccgca
      601 gaagcggatc ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc
      661 gtcgatgcgc tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaacta
      721 catctggaaa tgacgaccgt tcgcgcgcca tttgatggcc ggatcatttc cctcaaaacc
      781 tccgtcgggc aatttgcttc tgccatgcgc cctattttta ccctcatcga cactcgtcac
      841 tggtacgtga tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc
      901 gcaacgattc gcctgatgag tgacagcggc aaaaccttcg agggtaaagt ggattcgatt
      961 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc aaaagtgtct
     1021 cgttctatta actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa
     1081 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
     1141 tgagcgcgct caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag
     1201 agttaagcga gcggcgaccc ggccgcgtgc cgcagatcgt gcaactctgg gtaggctgcc
     1261 tgctggtgat tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag
     1321 tgctgtttta cggtattcag tcgaacgcgt tttacaccaa atttgtggcg atcttgtttg
     1381 tggttgccac ggtgctggag atcggcagcc tgtttttgat ctacaaatgg tcatacggcg
     1441 aaccgttgat ccgattgatc atcgccgggc cgatcctgat gggctgcatg tttttgatgc
     1501 gcacccatcg gctgggactg gtctttttcg ctgtcgccat ttacgggcaa accttccccg
     1561 ccatgctcga ctatccggaa gtagtcgtgc gcttaacgct gtggtgtatc gttgttggcc
     1621 tctatccaac cttgctgatg acgttaatcg gcgtgctgtg gtttcccaat cgtgccatta
     1681 cgcaaatgca tcaagcgctt aatgatcggc ttgatgatgc cattagccac ctgacggaca
     1741 gcctcgcacc gctacccgaa acgcggattg aaagagaggc gctggcgctg caaaaactga
     1801 atgtcttttg tctcgcggac gatgccaact ggcgaactca aagcgcatgg tggcaaagct
     1861 gcgtggcaac ggtaacctac atttactcga cgctgaatcg ctacgatccc acctcttttg
     1921 ctgattctca ggcaattatt gaatttcggc aaaaattagc ctcagaaatc aacaagctgc
     1981 agcatgccgt tgctgaaggt cagtgctggc aaagcgactg gcggctcagt gaaagtgaag
     2041 cggtggcggc acgggaatgt aacctggaga atatctgcca gacgttgtta caactgggcc
     2101 agatgaaccc gaatacgccg ccaacgcccg ccgccaaacc gccatcaatg gtcgccgatg
     2161 cttttaccaa tccagactat atacgctacg cggtaaaaac attgctcgcc tgtttgatct
     2221 gttacacctt ttacagcggc gtggactggg aaggcattca cacctgtatg ctgacctgcg
     2281 tgatcgtcgc taatccaaat gtcggttcgt cgtaccagaa gatggtgctg cgttttggcg
     2341 gggccttttg cggcgcgatt ctggcgctgt tattcacgct actggtcatg ccctggctgg
     2401 acaatattgt cgaattgctg tttgtgctgg caccgatttt cctgttgggc gcatggattg
     2461 ccaccagctc tgaacgctct tcttatatcg gcacacagat ggtggtcacc ttcgcgctcg
     2521 ccacgctcga aaacgttttt ggcccagtgt acgacctggt ggaaattcgc gatcgcgccc
     2581 tgggtatcat cattggtacc gtggtgtccg cgatgattta cacctttgtc tggcctgaaa
     2641 gtgaagcgcg cacgctgccg caaaaactgg ctggcgcgct gggtatgtta agtaaagtaa
     2701 tgcggatccc acgccagcag gaagtcacgg ctctgcgcac ttatctgcaa attcgtatcg
     2761 gtctgcatgc ggcgtttaat gcctgtgaag agatgtgcca acgcgtggcg ctggagcgtc
     2821 aactggacag cgaagaacgc gccttactga ttgaacgttc gcaaacggtt attcgtcagg
     2881 gccgcgatat ccttcacgcc tgggatgcca cctggaattc ggcgcaggcg ctggataacg
     2941 cactacagcc ggacagagca ggtcagtttg ccgacgccct ggagaaatac gctgccggtc
     3001 tggcaaccgc actcagccgt tctcctcaaa taacgcttga agagacaccc acctcgcagg
     3061 ccatcctgcc caccttatta aaacaggagc aacacgtctg ccagcttttc gcccgcttgc
     3121 cagactggac agccccggca ttaacgcccg ccacggaaca ggcacaagga gccacgcaat
     3181 gatcaatcgt caactttcac gtctgctgtt gtgcagcatt ctcggcagca cgacgctgat
     3241 ttccggctgt gccctggtac gtaaggattc tgcgcctcat caacagctca aaccggaaca
     3301 aatcaaactg gccgatgata ttcatcttgc cagctccggc tggccgcagg cgcagtggtg
     3361 gaaacaactc aatgacccac agttggatgc cctgatccaa cggacgctaa gtggttcaca
     3421 caccctcgcc gaagcgaaac tgcgggaaga aaaagcgcaa tcgcaggccg atttgttaga
     3481 tgccggttca cagttgcagg tggcagcgtt agggatgctt aaccgccaac gtgtctcggc
     3541 gaacggcttt ttaagccctt atgcgatgga tgcgcccgca ctggggatgg acgggccgta
     3601 ctatacggaa gccacagtag gtttgtttgc cggactggat cttgatttgt ggggtgtgca
     3661 tcgctcagcg gttgccgccg ccattggcgc gcataatgcc gcgctggcag aaaccgcagc
     3721 agtagagcta tcgctgacca cgggcgtagc gcagctttat tacagtatgc aggccagcta
     3781 tcagatgctc gatctgttag aacaaactcg cgatgtgatt gattacgcgg tgaaagcgca
     3841 ccaaagtaaa gtggcgcacg gtctggaagc acaagtgcct ttccacggcg cgcgggcaca
     3901 gattctggcg gtcgataaac aaattgctgc cgtcaaaggg caaatcaccg aaacgagaga
     3961 atctctgcgc gcattgattg gcgcgggagc cagcgatatg ccggagatca aacctgtggc
     4021 attaccacga gtccagaccg gcattccggc gacactctct tatgagttgc tcgccagacg
     4081 cccggatctg caagccatgc gctggtatgt tcaggcgtca ttagatcagg tggattccgc
     4141 gcgggcgttg ttctatccga gctttgatat caaagcgttt ttcggccttg atgccatcca
     4201 cctggatacc ttattcaaaa aaaccagtcg ccagttcaac ttcatcccgg gtctgaaatt
     4261 gccgctgttt gacggtggac ggttgaatgc caatctcgaa ggcacgcgcg ccgccagcaa
     4321 catgatgatt gaacgttaca accagtcagt actgaacgcg gtgcgtgacg ttgccgtcaa
     4381 cggcacgcgt ctgcaaacgc tcaacgacga gcgagaaatg caggctgaac acgtggaagc
     4441 aacgcgcttc acccagcgcg ctgccgaggc cgcctatcag cgcggcttaa ccagccgctt
     4501 acaggccact gaagcccggt tgccagtact tgccgaagag atgtcattac tgatgctgga
     4561 cagccgccgg gtgatccaaa gcattcagtt gatgaaatcg ctgggcggcg ggtatcaggc
     4621 ggctcccgtc gtcgagaaaa aataaaatgt ctgccgcgtg atggctgtca cgcggtattt
     4681 cgtttcgtca cgtcaaaact gacgacagcc tgtttttcgt cagagttttg aataaatagt
     4741 gcccgtaata tcagggaatg accccaaata aaatgtggca taaaagatgc atactgtagt
     4801 cgagagcgcg tatgcgtgat ttgattaact ggagcgagac cgatgaaaaa agtcgtcacg
     4861 gtttgcccct attgcgcatc aggttgcaaa atcaacctgg tcgtcgataa cggcaaaatc
     4921 gtccgggcgg aggcagcgca ggggaaaacc aaccagggta ccctgtgtct gaagggttat
     4981 tatggctggg atttcattaa cgatacccag atcctgaccc cgcgcctgaa aacccccatg
     5041 atccgtcgcc agcgtggcgg caaactcgaa cctgtttcct gagatgaggc actgaattac
     5101 gttgccgagc gcctgagcgc catcaaagag aagtacggtc cggatgccat ccagacgacc
     5161 ggctcctcgc gtggtacggg taacgaaacc aactatgtaa tgcaaaaatt tgcgcgcgcc
     5221 gttattggta ccaataacgt tgactgctgc gctcgtgtct gacacggccc atcggttgca
     5281 ggtctgcacc aatcggtcgg taatggcgca atgagcaatg ctattaacga aattgataat
     5341 accgatttag tgttcgtttt cgggtacaac ccggcggatt cccacccaat cgtggcgaat
     5401 cacgtaatta acgctaaacg taacggggcg aaaattatcg tctgcgatcc gcgcaaaatt
     5461 gaaaccgcgc gcattgctga catgcacatt gcactgaaaa acggctcgaa catcgcgctg
     5521 ttgaatgcga tgggccatgt cattattgaa gaaaatctgt acgacaaagc gttcgtcgct
     5581 tcccgtacag aaggctttga agagtatcgt aaaatcgttg aaggctacac gccggagtcg
     5641 gttgaagata tcaccggtgt cagcgccagt gagattcgtc aggcggcacg gatgtatgcc
     5701 caggcgaaaa gcgccgccat cctgtggggc atgggtgtaa cccagttcta ccagggcgtg
     5761 gaaaccgtgc gttctctgac cagcctcgcg atgctgacca gtaacctcgg taagccgcat
     5821 gcgggtgtaa acccggttcg tggtcagaac aacgtgcagg gtgcctgcga tatgggcgcg
     5881 ctgccggata cgtatccggg ataccagtac gtgaaagatc cggctaaccg cgaaaaattc
     5941 gccaaagcct ggggcgtgga aagcctgcct gctcataccg gctatcgcat cagcgagctg
     6001 ccgcaccgcg cagcgcatgg cgaagtgcgt gccgcgtaca ttatgggcga agatccgctg
     6061 caaactgatg cggagctgtc ggcagtacgt aaagcctttg aagatctgga actggtcatc
     6121 gttcaggaca tctttataac caaaaccgcg tcggcggcgg atgtcatttt gccgtcaacg
     6181 tcgtggggcg agcatgaagg cgtgtttact gcggctgacc gtggcttcca gcgtttcttc
     6241 aacgcagttg aaccgaaatg ggatctgaaa acggactggc aaatcatcag tgaaatcgcc
     6301 acccgtatgg gttatccgat gcactacaac aacacccagg agatctggga tgagttgcgt
     6361 catctgtgcc cggatttcta cggtgcgact tacgagaaaa tgggcgaact gggcttcatt
     6421 cagtggcctt gccgcgatac ttcagatgcc gatcagggga cttcttatct gtttaaagag
     6481 aagtttgata ccccgaacgg tctggcgcag ttcttcacct gcgactgggt agcgccaatc
     6541 gacaaactca ccgacgagta cccgatggta ctgtcaacgg tgcgtgaagt tggtcactac
     6601 tcttgccgtt cgatgaccgg taactgtgcg gcactggcgg cgctggctga tgaacctggc
     6661 tacgcacaaa tcaataccga agacgccaaa cgtctgggta ttgaagatga ggcattggtt
     6721 tgagtgcact cgcgtaaagg caaaattatc acccgtgcgc aggtcagcga tcgtccgaac
     6781 aaaggggcga tttacatgac ctaccagtgg tggattggtg cctgtaacga gctggttacc
     6841 gaaagcttaa gcccgattac gaaaacgccg gaatacaaat actgcgccgt gcgcgtcgag
     6901 ccgatctccg atcagcgcgc cgccgagcag tacgtgattg acgagtacaa caagttgaaa
     6961 actcgcctgc gcgaagcggc actggcgtaa tacagtcctt tctacagcct cctttcggag
     7021 gctgtttttt tatccattcg aactctttat actggttact ccctacccaa tcgtattatc
     7081 aaaatgaaaa aaattatcgc attgatgttg tttttgacat tctttgccca cgcgaacgac
     7141 tccgagcctg gcagccagta tttaaaggca gcagaggccg gggaccgacg tgcacaatat
     7201 tttcttgccg acagctggtt tagctccggc gatttgagca aagccgaata ttgggcacag
     7261 aaagccgccg acagcggtga tgctgatgcc tgcgcgctgt tggcgcagat caaaatcacc
     7321 aatccggtca gtctggacta tccacaagca aaagttcttg cagagaaagc ggcgcaagcg
     7381 ggcagtaaag aaggtgaagt aacgctggcg catattctgg taaatactca agcgggtaaa
     7441 ccggattatc caaaggcgat ttcgctgtta gaaaacgcct cggaagatct ggagaatgac
     7501 tccgccgtcg atgcccaaat gctgcttggt ttgatttacg ccaacggcgt gggcattaag
     7561 gccgacgatg acaaggcaac ctggtatttc aaacgcagct ctgcaatttc ccgaaccggt
     7621 tattccgagt actgggcggg aatgatgttc ttaaacggtg aggaaggttt tatcgagaag
     7681 aacaaacaaa aggcgctgca ctggttgaac ctgagctgta tggaagggtt tgataccggg
     7741 tgtgaagagt ttgaaaaatt aaccaacggt taaggtaggc ctgataagac gcggcaagcg
     7801 tcgcatcagg catccacaca ttgccggggg atatccccgg caatcttcaa ttattgatcc
     7861 gcagttttat caaatttgcc cagcacttca cgctcataag ccagcgcctt tttgcggtca
     7921 aatttgtgtt cccacttggc aatcaccagc accgccagcg cattacccac cacgttcagc
     7981 gcagtacgcg ccatgtcgag gatacggtca acaccagcaa taaacgccag accttccagc
     8041 ggaataccta cgctacccag cgttgccagc aacaccacaa acgacacgcc agccacgcca
     8101 gcaatccctt tcgaggtcac catcagcgtc agcaccagaa tgatttcctg ccagatggac
     8161 aggtcaatgc catacagctg agcgatgaaa atagcggcaa tactttgata cagcgtcgaa
     8221 ccatcaaggt taaaagagta accggtcggc accacgaagc tggtgatcga cgccggtgct
     8281 ccgtaggctt ccatcttctc aataattcgc ggcagtacgc tttcagagct ggcagtggag
     8341 tacgccagaa tcagctcatc tttcagaata cgaatcagga tccagacgct taacccgcac
     8401 agacgtgcca caattcccag cactaccagc gcgaagaaca gaatggcgaa atgcaccagc
     8461 agcaccagtt tcgccagtgg ccacagagac gagaaaccaa agttagccac cgtcaccgca
     8521 atcagcgcaa acacacccac cggtgcataa cgcatcacca tgtgagtcac tttaaacatg
     8581 gtttcagaga tggagcggaa cacggtcacc agcggttcac gatgcgtcgc gggcagggaa
     8641 gaaagcccca gaccaaacag caccgagaaa aagatgatcg gcagcatttc gcctttcgcc
     8701 atcgacgcca caatgttcgt cggcaccagc gacaaaatcg tgcccataat gccgtgggaa
     8761 ctgctttgta ccgcttccgt agtgctctga tatttcgaga tatcgacggt cgccagctgc
     8821 gacatatcca ccccggcacc gggctggaag acgttcgcca gagtgatccc caaaatgatg
     8881 gcgacggtgg tgatcacctc gaagtaaata atggttttcg cgccaatacg cccgagctgt
     8941 ttggcatcac caacacccgc gatacccacc accagcgtgg agatcacaat cggcacaaca
     9001 atcattttga tcagatggat gaagatatca cccgccggag agagcaaatt gacgaccagc
     9061 cagtcgcggc tgtcgctatg gtagtgcagg tagcttccca ggagaatgcc cagcaccata
     9121 gcaaacagaa tctgccaggc caggctgaat tttatatttt tcatcagaat aacttcctca
     9181 atggagcgcc gttccccgtt cagaaacgcg agaacgtgac atactgccag ggcatgaatt
     9241 gtgttgcgtt ggtttgtaga tttttttaac gcttatgatc agtatcattt gcgcggtatg
     9301 ttgcgcaacc ctttaacaac agagcttttc gctgcattaa tgcataataa cgcgagttta
     9361 tggcaggatt aatgaataac catttgttat ggaaagattt ttatttgttt aatctgcaat
     9421 aatactgatt ggtgattatt tttggcatat gttcattcga ttatttttta tgcagctcaa
     9481 tgacataatc ttcatcacca gatgattaat ccgatctccg ctgcaacagt tttgccatat
     9541 taatcgactc gaccagctgt gtccggttga tccggggtga gttgagatcc atcacttttt
     9601 gccatagttc gatggcttgc gcgtaattcg cctgcataaa cgcatcggaa gccagcagca
     9661 tcagagcggt gatttcgtta gagtccagcg cgagggcttt gtcgatcatt gcgcgagtct
     9721 gggcggtcat atgctggctg gcctggtaat aaagcaccgt cgccagcgcc gcatacagtt
     9781 cagcgttctc accacgtagt tgcaacgcct gacgatacgc cagcagcgaa ttgctgtaat
     9841 cgttttgcca cagatagtac tcgcccagta acgcccattg ttcgctgttt tgtggattag
     9901 cgcggatttt atcctgcaat gcctgaagct gcgcttccgg ggtttgctgg ctggcaaact
     9961 gatgtagcgg atcgcgctga cgctgatact ccgcacgtac cgcctgccat tttgggctta
    10021 acagataact gccgacacag aggaatacca tcaatatcgt gagtgttgtc agcattttca
    10081 ccggtatttg aggctgtttc atcgcttagc cctcactcgc cagaggatca gtgccatcag
    10141 caataacaac accactggca gcgcccacaa caccagcgtc tgacccgtta acggtgggtt
    10201 atagcgaaca aaatctccgt agcgttcggt catccagcca atgatttcga cttcgttctt
    10261 cccctccgac accatgctat aaacctgatg gcgcatactg accgccaccg gcgcattgga
    10321 ttcgagtaag ttttgattct ggcactgcgg acaacgtaac tggctggcaa tatttaacgc
    10381 ctgttgctgt tgttgcggat tggcgaattg ccaggtgtct acaacctgag cgcgcgcaaa
    10441 acaggtaaat aacagcagta gcgtgagaag ccctttattc atcgcgcttc ttcccccgcc
    10501 atccgcttaa caatgcgccc gcaatcatca acaatcctcc cccccagatc cagcgcacac
    10561 cgctttgtac atacaaacga aaagcgtaac gatccgctct ggttttttca cccatgaccg
    10621 cataccagtc atggatgccg ttccagcgaa ttgacggttc catcatttgc tgacggcgtg
    10681 cttcataaaa acgccgctcc ggcgttaatt caccaatgcg ttgctgatgg tcaaacagcg
    10741 ccactatcgc tttttcgctg gtgtaattgc ctctggcttg cagatcgagt cgctcaaaac
    10801 ggaaggtgta tcctgccagc gtcacctgct gacccggctg taaattcagg ctgatctcct
    10861 gacgactgac actggaaacc acgatccccg cggcaaataa cagcacgcca gcatgagcta
    10921 ccagcgccgg aagctgcacg cgtttgccag agacaaacgt cgccagcaca atcaccacca
    10981 gcatcaacag accaaacggt aacgtcgcgc ggttaaaata cggcgcgcca acggagaggc
    11041 gtccccagcc cagcaggccg taaatcatcg gataaagcgt tcccaccagc acgatcagta
    11101 gcactgcgca aaacagcaac agcgtagcga ggattaacat ttcccgcgat aaccccgaaa
    11161 aacgcaccgc cgggccaccg tcccgcgctc gccagccata cagagccaaa gacgcaaggc
    11221 taatcagtgc aaacaggctg aacaacggca cggcgcggac gttatccagt gcgaacgcat
    11281 gaaccgaaac cagaatgcca gaacggacaa ttaaggtgcc cagcagcgac agcatcagag
    11341 taactatcgc cagcaacagc gaccagtggc ggaaaatccc ccgctggcgt gtcagggaca
    11401 aactgtgcag cagcgcagtg gcagaaagcc agggtaataa agaggcgttt tccaccggat
    11461 cccagaacca ccagccgccc cagccgagtt cgcagtaggc ccaccaggaa ccgaggatga
    11521 tccccgccgt taatgcactc cagccaggta acgcccagcg ccagcaaatt cgggcgctgg
    11581 ccgcatcaaa ctcgccgcgc agtagactcg ccagcgccac gctcgccgct accatcaaac
    11641 cgccatagcc aaggtaaagc agcggtggat gaaagataag accgggatgt tgcagcatcg
    11701 gattgagatc gcggccttcg attgctggtg gaaatatccg cacgaaggga tcggaccaca
    11761 gcaccacaaa cagtagcagt gcggcgagca tgagagataa aacggctagc gtcagcggaa
    11821 atagcggatc gctttgctgc cgataatgcc aggcaaacag cgcgctccag gcggaaagca
    11881 gcagcaccca aagcagcagc gaaccttcat gaccgcccca caccgccgcc agtttgagtt
    11941 cccacgacag caggctgtag ctatgttggg cgacataaat gaccgagaaa tcgctgatga
    12001 gaaaacaata cgtcagtacg ccaaaggcga gcagcaggag cgcgaactgc gccagaatgc
    12061 cgatgcaagt gagtcgcatc atggcaggcc agcgcaaccg cactcccgca aaggccgtca
    12121 acggggtcaa cacgttgacc ccgagactta acaacaacgc cagaaaaccg acttcaggaa
    12181 ggaatacgtc cagaaaccac ctcttttctg gcttacgcca ccgtcaactg tccggcgtag
    12241 agaatgaaaa agcgcagcat caacacgccg accagactgg ccccacacgc cgccagcacg
    12301 gcaggaatgc cggaactgcg attgacccac ggtttgagca acattggcac aatcagcccc
    12361 agcccggcga caccaagcca gaaccaccac gtccagaaac cgccacctaa tgccgccacc
    12421 agcgcacgaa ctttaccgtc atcgcccagc gccagaccga caaaaaacgc caccagcagg
    12481 aagatttcac cccataccac ggggatttcc atccggtgaa caaagtgcgc ttccgtggaa
    12541 tgcgggttac tgcgttggcg tatcgccatg gcgatcagcg ccaccgccgc accggacgag
    12601 atgccggaga agaggagcag caccggcagg ctcgggttat tgaggaacgg atacgatttc
    12661 agcgccgaca gcagaaagcc ggtataagcc cccagcagca ccgccaacac cagcatcagc
    12721 gtttccagtc cgcgatgaac gggcgtcagc aggctcagaa ccttttgcac gatccccagc
    12781 ttcggcaacc agcgttgttg cagggcaagc aaatcatgtt caaagatttt cgccagccac
    12841 agcaccagca ccaccatgta gagctgaaac agcatcaccc ccatcgacat caccgaggta
    12901 aaactgtagt ggaacatcag cttccagaag gtccacggtc ttgtcaggtg gaagacgagg
    12961 atcaacagac cgaggatcac cgcgcccggc ccgacaatca gcgtggtgcg cagcaacgta
    13021 ctgtctgcac cgcccgcctg cgggtagaag cgacgtaaca gcacggccag cgtcaccaga
    13081 ccggcagaaa taccaatcaa aaacaggtag atggcaatcg gccagtccca caccagcgat
    13141 tcaaaatgaa atgcggaagt ctgcgtcatt ggctcacctc cccgtattta aacggtacgc
    13201 ggtacagctt cggtttggtt cccagcgcca gcttgtagcg gtaagtaggc ttctggcgca
    13261 gcagttgcga aatctcactg ttaggatcgt ccagattgcc aaacgtcagc gccttcgtcg
    13321 ggcaagcttc aacgcacgcg ggaagcttac cggcctgcaa attggtctta cgacagaaat
    13381 cgcatttgtc cgccgtcttc gtgaccggat ggataaagcg cacgcggtac ggacaggcgg
    13441 cgatgcagta ctgacaaccg acgcagagat caggattaac atcgacaatc ccgctagcgg
    13501 catcacgaaa agacgcaccg gtcgggcaga cgtcaacgca cggcgcatga tcgcagtgct
    13561 ggcaagactt acggaagaag cgatatttca catcaggaaa ttcgccctgc ggctcgctac
    13621 gaataatcgt caggcgcgag acgccttccg gcaccttgtt cacttcccga caagcatcca
    13681 tacaggcggt acagccgatg cataacgact cgtcatgcac catgccgtaa cgcaccccat
    13741 tgatattcaa cgttttcgcc accacgcgcc ctgcggtgcc gctgacggct gccagcacgc
    13801 cgacgccggt gagaaactgg cgacgagacc aggtcatggc tgctccttaa gcaacggaac
    13861 agacgccggg ttaaagttcg gattggtgcg ctgatcgctg tggcaatcga cgcaaatctt
    13921 aatccgtcct ttgtcactta acgtctgcat cgtatcttgc tgtggatgca gggaatggca
    13981 gctggcgcac gccactttgg ttacgtggac atcgtgcggc cagaacgctt tttgcaactg
    14041 ttccggcaga tgacaggaca tacagacgct gttctgctct ccgaccttat acatcggctc
    14101 gttaaagcgc atcacatctt tcaccccttc gcggtgttgc ggtgatgctt ggccgtgaca
    14161 gttggtgcag gtgaccggca atttgttatt cgggttgata acggaagcat gtttgccatg
    14221 cataccttcg gtatctggtt tatgacagtc cagacaggcg gcatccgggt tacgctgctg
    14281 ggtaacttcg tagcgatcac cagatgcctg caccgcaggg gtagcggtaa ttccgttcag
    14341 gctccacaac aggcccgacg ccagcacccc agcagttaat aacgaacgta atacgctcat
    14401 attcactcca ttgtgttttc cgccccttgc gaggcggaac ggggttattg gcttaacaga
    14461 ccgtttttac gtgcctgctc ttcccactgc gggatcaccg ttttgatgaa gtcctgcttc
    14521 tcggccttga tttgttccat gttcaggcca atggcctgct gggctttctc tttggtcgag
    14581 atatccggga tctggatttc atgggtgata cctttggtcg ccagcaggcg cgccagtttg
    14641 gtgcgcgcat ccgccgcttt atccatcgcc gtaccgagca tccgtagacc ttcttccggt
    14701 gcgtgcatat gaataccgtg ggaagcgatc gccagatccc aacgccactg ggcatgacgg
    14761 atatcgtcct gaatcggctt catttcggct tccgtcgcgc ctgcatccag cgccgctttc
    14821 gcttcgaagt gagcgtgaac cagttgatct tcaaccttga ttttcaggtc gttaatcgat
    14881 tgcttacgtt ccgcgaccac tttttgcaag gcagctttat cctgggtatg gcagttcgca
    14941 caagtctggg cgaagttatc aaacggatta ccaattttat ggtcggtgta gagtttgcct
    15001 tcggcgttct gcacttttgg catatggcag tcgatacagg tcacgttgtt tttaccgtga
    15061 atgcccgctg tccaggtttc atattccggg tgctgcgctt tcagcattgg cgttttcgac
    15121 agggagttag tccagtcaga gaaggcaatt ttgtcgtaat actgctccat attttcgact
    15181 ttcatgccgt catcccacgg gaatttaacc gctttgtttt tgccgtcgaa gtaatactcc
    15241 acatggcact gaccgcaaac catcgattgc tggtcgaaac gtccggcttt ctcaaaaggt
    15301 ttaccaatgg cttccatcgc gcgagccgca tacggacggg aaagggttaa ctccggtttg
    15361 cctttggcga actctggtga ggcggtgtta tggcagtcgg cacaacctaa gttgttgacg
    15421 atttccgggc cgccgcgcgc ccatttaccg tggaagtagc catcttcgcc gtctttctgg
    15481 atcagacgcg ccacatccgg gcttttacaa ctccagcacg ccatcggtag cggaccatct
    15541 tcagcgtttt tcggcgcacc ggtacgcagg gtttcacgca catcggtcac agcaaaggca
    15601 tgtccacgag gcttgttgta atcgcgcgag aagggatacc ccgcccacag gatcaccagc
    15661 cgtggatctt ccgccagggc gtcaacacgc tctgactgtt ccgaggtggc tttccaggag
    15721 agatattgat cgtgatgctg cggggcaaag gtttcattct tcgcttccac agttacaggt
    15781 tttgcgggag ctgccgtttg ttcagcgtga acagaagtga aaaagaaaaa aggaatcaat
    15841 aagctgaaga tacggcgtgc gtttattttt atccttgtca taggggcttc atccgaattg
    15901 cgccattgtt gcaatggcgg tttttattgt ttttcacgac agtaaccgca cctacactgt
    15961 catgacattg ctcaccccta tgtgtaacaa ataaccacac tgtgaatgtt gtctttaatc
    16021 aattgtaagt gcatgtaaaa taccacttta gagttagtca gtatcttcct ctttttcaac
    16081 agcatgcata actgcatgtt cctcaaagaa ttaatcaact tttgttggtg accttcaaaa
    16141 attaccctgc cgtttatttg caccattcta cttttgcgtg atctgtcgcc caaatactaa
    16201 acaaaactgc caatacccct acatttaacg cttatgccac atattattaa catcctacaa
    16261 ggagaacaaa agcatgagcc aaattcacaa acacaccatt cctgccaaca tcgcagaccg
    16321 ttgcctgata aaccctcagc agtacgaggc gatgtatcaa caatctatta acgtacctga
    16381 taccttctgg ggcgaacagg gaaaaattct cgactggatc aaaccgtacc agaaggtgaa
    16441 aaatacctcc tttgcccccg gtaatgtgtc cattaaatgg tacgaggacg gcacgctgaa
    16501 tctggcggca aactgccttg accgccatct gcaagaaaac ggcgatcgca ccgccatcat
    16561 ctgggaaggc gacgacgcca gccagagcaa acatatcagc tataaagagc tgcaccgcga
    16621 cgtctgccgc ttcgccaata ccctgctcga gctgggcatt aaaaaaggtg atgtggtggc
    16681 gatttatatg ccgatggtgc cggaagccgc ggttgcgatg ctggcctgcg cccgcattgg
    16741 cgcgatgcat tcggtgattt tcggcggctt ctcgccggaa gccgttgccg ggcgcattat
    16801 tgattccaac tcacgactgg tgatcacttc cgacgaaggc gtgcgcgccg ggcgtagtat
    16861 tccgctgaag aaaaacgttg atgacgcact aaaaaacccg aacgtcacca gcgtagagca
    16921 tgtggtggta ctgaagcgta ctggcggaaa catcgactgg caggaagggc gcgacctgtg
    16981 gtggcacgac ctggttgagc aagccagcga tcagcactag gcggaagaga tgaacgccga
    17041 agatccgctg tttattctct atacctccgg ttctaccgga aaaccaaaag gcgtactgca
    17101 cactaccggc ggttatctgg tgtacgcggc gctgaccttt aaatatgtct ttgattatca
    17161 tccgggtgat atctactggt gcaccgccga tgtgggctgg gttaccggac acagttattt
    17221 gctgtacggc ccgctggcct gcggcgcaac cacgctgatg tttgaaggcg taccgaactg
    17281 gccgacgcct gcccgtatgg cgcaggtggt ggacaagcat caggtcaata ttctctatac
    17341 cgcacccacg gcgatccgcg cgctgatggc ggaaggcgat aaagcgattg aaggcaccga
    17401 ccgttcgtcg ctacgcattc tcggttccgt gggcgagcca attaacccgg aagcgtggga
    17461 gtggtactgg aaaaaaatcg gcaacgagaa atgtccggtg gtcgatacct ggtggcagac
    17521 cgaaaccggc ggtttcatga tcaccccgct gcctggcgct accgagctga aagccggttc
    17581 ggcaacacgt ccgttcttcg gcgtgcaacc ggcgctggtc gataacgaag gtaacccgct
    17641 ggaaggggct accgaaggta gcctggtgat caccgactcc tggcctggtc aggcgcgtac
    17701 gctgtttggc gatcacgaac gttttgagca gacctatttt tccaccttca aaaatatgta
    17761 tttcagcggc gacggcgcgc gtcgcgatga agatggctat tactggatca ccgggcgtgt
    17821 ggacgacgtg ctgaacgtct ccggtcaccg tctggggacg gcagagatag agtcggcgct
    17881 ggtggcgcat ccgaagattg ccgaagccgc cgtagtaggt attccgcaca atattaaagg
    17941 tcaggcgatc tacgcctatg tcacgcttaa tcacggggag gaaccgtcac cagaactgta
    18001 cgcagaagtc cgcaactggg tgcgtaaaga gattggcccg ctggcgacgc cagacgtgct
    18061 gcactggacc gactccctgc ctaaaacccg ctccggcaaa attatgcgcc gtattctgcg
    18121 caaaattgcg gcgggcgata ccagcaacct gggcgatacc tcgacgcttg ccgatcctgg
    18181 cgtggtcgag aagctgcttg aagagaagca ggctatcgcg atgccatcgt aacccacaat
    18241 tgccggatgc gaatcggtaa cggtttgtag gcctgataag acgcgacagc gtcgcatcag
    18301 gcattgattg ccggatgcgg cgtaaacgcc ttatccggcc tacattcggc aagggttacc
    18361 cgagcgttaa ccttctccca taagggagcg ggaattaaaa caatccctac attacctctg
    18421 gagaatctgt gatgaatggt acgatttatc agcggataga agacaatgcg catttcaggg
    18481 agttagtcga aaaacggcaa cggtttgcca ccatcctgtc gattattatg ctggcagttt
    18541 atatcggctt tattttactg atcgccttcg cgcccggctg gctgggcacg ccgctgaatc
    18601 cgaacaccag cgtcacacgc ggtattccaa ttggtgttgg agtgattgtg atctcctttg
    18661 ttctcaccgg tatctacatc tggcgggcga acggcgaatt cgaccgtctt aataatgaag
    18721 tcctgcatga ggtacaagca tcatgaaaag agttctgacg gcgcttgccg ccacactccc
    18781 tttcgcagct aacgccgcgg atgctattag cggggccgta gagcgccagc caacgaactg
    18841 gcaggcgatt attatgttcc tgattttcgt cgtgtttacg ctcggcatta cctactgggc
    18901 atcaaaacgc gtacgttctc gtagcgacta ctacaccgca ggcggcaata tcactggctt
    18961 ccagaacggg ctggcgattg ccggggacta tatgtccgcc gcctcattct tggggatctc
    19021 cgcgctggtg tttacctccg gctatgacgg gctgatctac tcgttgggct tcctggtggg
    19081 ctggccaatc attctgtttc tgattgccga acgtctgcgt aacctgggac gctacacctc
    19141 tgccgatgtg gcctcttatc gtctgaaaca agggccgatt cgtattcttt cggcctgtgg
    19201 ttctctggta gtggtggcgc tttaccttat cgctcagatg gtaggcgcag gtaaactgat
    19261 cgagctgctg tttggcctta actatcacat tgcggtggtg ctggtcggcg tgctgatgat
    19321 gatgtacgtc ctgttcggcg gcatgctggc gaccacctgg gtacaaatta tcaaagctgt
    19381 gctgttgctg ttcggtgcca gctttatggc ctttatggtg atgaaacacg tcggctttag
    19441 cttcaacaat ctgttcagcg aagcgatggc ggtacacccg aaaggtgtcg acatcatgaa
    19501 accgggcgga ctggtgaaag atccgatctc cgcgctctct ctggggctgg gactgatgtt
    19561 tggtacggcg ggcttgccgc acattctgat gcgcttcttt acagtcagcg atgcccgcga
    19621 agcacgtaag agcgtgttct acgccaccgg atttatgggc tacttctata ttctgacctt
    19681 tattatcggc ttcggcgcga tcatgcttgt tggtgcgaat ccggaatata aagacgcggc
    19741 gggccatctg attggtggta acaacatggc ggccgttcac ctggcgaatg cagtgggcgg
    19801 caacctattc ctcggtttta tttcagcggt tgctttcgcc actatcctcg cggtggttgc
    19861 ggatctgacg ctggcgggcg catcggcggt ttcgcatgac ttgtacgcta acgtcttcaa
    19921 aaaaggcgcg accgaacgtg aagagctgcg ggtatcaaaa atcaccgtac tgatcctcgg
    19981 cgtgattgcg attatcctcg gcgtgctgtt tgagaatcag aacatcgcct ttatggtagg
    20041 ccttgcgttt gccatcgcgg cgagctgtaa cttcccgatc attctgcttt ccatgtactg
    20101 gtcgaaactg actacccgtg gcgcgatgct gggcggctgg ctggggctga ttaccgcagt
    20161 ggtgctgatg atcctcggcc cgactatttg ggtacagatc ctcggtcacg aaaaagccat
    20221 cttcccgtat gaatacccgg cgctgttctc tatcagcgtg gcattcctcg gcatctggtt
    20281 cttctcggca accgataact cagcggaagg ggcgcgcgag cgtgaactgt tccgcgcgca
    20341 gtttatccgc tcccagaccg gctttggcgt tgagcaaggc cgcgcacatt aatctctccc
    20401 cttccccggt cgcctgaccg gggaatacgc ttcctctcca acatgcatca ccttttccca
    20461 taatattaaa caaataaact cattaaaaaa tgagcgattt ttgacagtcg tagaaaatga
    20521 taatgcagag aatatgcctt ttctttcttg ttaattataa ggatatttta tgcgctataa
    20581 tggtttaaat aatatgtttt tccctctttg ccagattaac gataaccact ctatcacaag
    20641 ttcatcacat acaaagaaaa caaaatcaga taattacagc aaacatcata aaaacacgtt
    20701 aattgacaat aaagccctct ctcttttcaa aatagatgat catgaaaaag tgataggctt
    20761 gattcagaaa atgaaaagaa tttatgatag tttaccatca ggaaaaatca cgaaagaaac
    20821 ggacaggaaa atacataaat attttataga tatagcgtta tatgcaaata ataaatgtga
    20881 cgatagaatt acgagaagag tttaccttag taaagaaaag gatgtatcca ttaaggtggt
    20941 atattttata aataatgtcg ccgtccataa taatactatc gaaattccac agatagtaaa
    21001 tggtggttac gatttttcac accttagcct gaagggtatc gtgattaaag atgaagattt
    21061 atccaattcg aattttgcag gttgcagact acaaaacgct attttccagg actgtaatat
    21121 gtataaaacg aatttttatt acgccataat ggaaaaaata ctttttgata attgtattct
    21181 cgatgactca aatttcgctc agataaaaat ggccgacgga actctaaatg catgctccgc
    21241 tatgcatgtt caattctaca atgcagcaat gaatagagcc aatattaaaa ataccttcct
    21301 tgactattca aatttttata tggcgtacat ggctgaggta aatctttata aagtaatagc
    21361 gccatatgtt aatttattta aagccgacct tagtttctct aaactcgatt taattaactt
    21421 tgaacatgct gatctgtctc gcgtcaatct gaacaaagca atcctccaga atataaactt
    21481 aattgatagc aaactctttt gtacgtggct gacaaataca ttcctcgaaa tggttatatg
    21541 taccggctct aatatggcta atgttaattt taataatgcc aatttaagca actgccattt
    21601 caactgttct attttaacaa aagcctgtat gtttaatacc cgtctctatc gggttaattt
    21661 cgatgaggct agcgtccagg gaatgggcat ttccattctc cgtggggagg aaaatatccc
    21721 cattgatagt gataccctgg taacactaca gaaattcttt gaagaagatt gtacctctca
    21781 tactggcatg tcacaaactg aggataatat taatgcagtc gctatgaaga ttactgcaga
    21841 tattatgcaa cacgcagatt gaggttaagt cgaagaacag ccaatgaatt gactttacaa
    21901 gagagataac ttatttgttt tagaatcatg aatgttcgcc cgagctaacc tgttaaaggt
    21961 gcaaaagatt gcaccttttt cagattacct cgggcctggt tacgtcgctt tcggcttact
    22021 gattttcctc aattagcaac gcttcaagca aatcgagatc gtgcagtaat ttttgcagcg
    22081 tttcgttgct gatctcccgc gtggcgcgca ggtggtacag ttcagcacgt tcagaacgca
    22141 atgccgccag acggaagcga cgctcaaggt tctcttcctg cacggaactt tcaacgtcgt
    22201 tacgtccatc ggcgcgacga cgcaggttac caatgacgcg agaactgacc tccgtaagca
    22261 actggttatc gatattctct tcggtatccg ccgccagacg ctcctccatt ttctggatcg
    22321 ccacaatcgc cacttctgcc gttgccgctc gcgcaatacg ttcttctttc aattgttgcg
    22381 aatggtccgc gacttcaatg tgttgtagca gaataggcaa catcaccacg ccgacaaaca
    22441 gcgagaagag aatgacgcca gccgccagga acaccagctc atagcgcgcc gggaagacgt
    22501 taccatccgg caagagcagc gggattgaga gcacaccggc cagagtgatc gccccacgca
    22561 ccccagcgaa agacgcgatc aggatttctc gtgtggtcca cgaaccaaac tccatcggct
    22621 tcttcttcag gaagcggttg ctgaactttt tcatcgtcca cagccagccg aaacggacca
    22681 gcatcagcgc cgcatatatc agaataatat cggtaaacag catccagatt tcgacgttag
    22741 ggtcgatttc tgccgccatc agcgacgtct ccagaatacc cggcagttgc agacctaaca
    22801 gcaggaacac catgccgtta aagacaaatt ccagcatcgc ccaggtgctg tttgcgcgca
    22861 ggcgcattgc cagtggcgca cggcgcatca caccggagcg ggtgatggtc atcccggcgg
    22921 caaccgcagc gaggatgccc gaaacgccaa tatgttcggc aatcagataa gaagcgaatg
    22981 gcagcaagaa cagcagaacg atctgcgtcg cgggttcatc accgccccag cggctgagga
    23041 atcgcagcga gcgaccatac agccagctca ccacaaaacc ggcaagaata ccgccaatgg
    23101 ctactttcat aaattcgacc gtcgcaccgc cgacagtgaa gatcatcgtc cccatcgcca
    23161 ctgccacggc aaacttcaac gacaccaggc cggaagcgtc attcatcaac gcttcgccct
    23221 gcaaaatgcc catgattttt ttcgggatgc gcccttcgcc aacaatcccg gagagcgcca
    23281 cagcatcggt cggagaaagt accgccgcca gcgcaaaggc ggggatcagc ggaatgcccg
    23341 gcaccaccca gtaaataagg aagccaatgc cgaccacggt gaccaccacc agcgccagcg
    23401 cgaggccgaa aatctctcga ccatgttcga gaaattcacg ggtcggcgtt ttccagccat
    23461 cagcgaacag caacggcggg ataaacaaga ctaaaaagag ttcaggatca aactccacat
    23521 gcaaaccaaa cgtcggccac gccagtagcg caccgatggc gatttgcata agcggaagcg
    23581 ggatctgaaa gggcatgaca cgagtgacca ccccggacag cgagaccacg agggtcatta
    23641 tcagtatggt gaagaagatt tccatgcgtt ccctgtttgc gatgtttttt atgatctgag
    23701 aaagacgtta tgcctgaatt ctcatttttt cagaatatcg caaaagctca cacaatgtgc
    23761 ggagaaaata aaaacgggca gccattgcca cccgttttca gaatccgatg aaagattaga
    23821 tagcccagcc acccgcatag aaggtcacca gcgccacggc gataacgacc gtaccgatgt
    23881 tcaacttgcg ccattcaccg gaaaccagac gaccaatcac cagagtcgcg aagccgatca
    23941 tgatgcctgt tacgatgtta caggtcagca cgatgaatac cgccgtaacc agacccgcca
    24001 tcgcatcaac aaaatcagca aagtcgattt tcgccacgtt gctcagcatc agcaggccaa
    24061 cgtacatcag cgccggagcc gttgcatacc ccggaacgag gtaagaaagc ggagagagga
    24121 acagaatcag caggaacagc acgccaacgg tgatagccgt caacccggtt ttaccgcccg
    24181 ccgccgtacc cgccgcagac tcgatgtata ccgctgccgg agctgcaccc accaggccag
    24241 agaaaacgct gctcatggag tcagtggtca gtgctttccc gccgtcgatg atctgcccat
    24301 ctttatccag caggttcgcc tggccggcga cagcgcggat agttccggtg gcatcaaata
    24361 ctgccgtcat caccagcgcc agaacgcttg gcaggactac aggattcagc gcgcccataa
    24421 tgtccaggct gccaatcagt gaattgccgt tttcatcgct caatgaaggc atggcgaaaa
    24481 cgccggagaa atggacgtta ggatcgaaga tcaaaccgac aattgagata ccgataatgg
    24541 tcagcagaat gccaccaggg actttcagtt tttccaagcc gatgatcacc gccagacctg
    24601 ccagtgacat aatcaccggg aaggtcgcga aatcacccag cgcaacgggc agaccatcaa
    24661 gcgggttttt aatcaccaga ccgacaccat tagcggcaat gagcagcagg aacagaccga
    24721 taccgatccc cgtgccgtgc gccacaccgt gcggcaagtt gcgcaaaatc cagctacgga
    24781 tacccgtggc agaaattacc gtaaacagca cacccatcag gaacacggca cccagcgcga
    24841 caggtacgct aatatgttgc cccagcacca ggctgaatgc ggtaaacgcc gtcagggaga
    24901 tggcgcaacc aatcgccaac ggcagattag cccacagacc catcacgata gaaccgagtc
    24961 cggcaaccag acaggttgca acgaaaactg ccgcaggcgg gaagcctgct ttacccaaca
    25021 tacctggaac gacgatgacc gagtagacca tcgccagaaa cgttgttaac ccggcaacca
    25081 cttcctgacg gacagtgctt ccacgttgtg aaattttaaa ccaggcgtcg agtgaaccgc
    25141 cggtacgcgc tgatggcgta gacataagaa aacatcccct gagagtttaa ttttcgtcaa
    25201 gatgcgtggc ggacaatccc ctgccagcga aacgttatct tcctgctcca ggtttgcggc
    25261 aatttggtgc gtcgcaaaaa agcaaacgtt tagctccgcg catacaaaac gggtggtaaa
    25321 aaaaagcaaa cgattatccg gcgatcatag cggctttttc aaccgaagtt tgctgctttt
    25381 ttctctttta ttgtcttgac gatgaaaaac caggcaattg atgcgcaaac gttatcgtcg
    25441 atgtgcaaga ttgcagcgtt atcgagccgt ataccgttct ggattgcgat aaaaaatcgt
    25501 ctgagatgac aaaaatattg cggctaaaaa gtatactttt acactggcga agaaacgtcg
    25561 gtgacataca attaaagcat caacaccaac cggaacctcc accacgtgct cgaatgaggt
    25621 gtgttgacgt cgggggaaac cctcctgtgt tccagcggga tagagagaaa gacaaagacc
    25681 ggaaaacaaa ctaaagcgcc cttgtggcgc tttagttttg tgtttgcgga taattccaaa
    25741 agagctcgat cagtcccctc gccccttcgg ggagagggtt agggtgaggg gaacaggcag
    25801 caccagtgca aatatttacc ctcaccccgg ccccatagtt aacgatgagt cagcttgtcc
    25861 ttgtggcgct ttagttttgt tcatcttcca gcaagcgtgc gccggtacct tcttctccta
    25921 agcggtcgcc cgggttacgc aacgggcaat cactgcgcga aaggcagcca caaccaatac
    25981 atccgtccag ttcgtcacgc agcgccacta aggtatgaat gcgccgatcc aactcttctc
    26041 gccattggga cgaaagctgt ttccactctt tcgcacttaa cgtatgccct tctggcaaca
    26101 cgccaaacgc ttcaccaatg gtcgccagcg gaatgccaat acgctgagcg attttgataa
    26161 ttgcgacata tcgcaacaca tcacgtttat atcgccgctg attgccgctg ttacggatac
    26221 tagtaatcaa ccctttgctt tcatagaaat gcagcgccga taccgccaca ccgctgcgtt
    26281 tcgccacttc gccaggggtt agcagcgctt taatgcgggg taatttcttt tccataaatc
    26341 gctttacctc aagttaactt gaggaattat actccccaac agatgaatta acgaactgaa
    26401 cactgaaaag aggcagattt atgtcccatc agaaaattat tcaggatctt atcgcatgga
    26461 ttgacgtgca tattgaccag ccgcttaaca ttgatgtcgt cgcaaaaaaa tcaggctatt
    26521 caaagtggta cttgcaacgg atgttccgca cggtgacgca tcagacgctt ggcgattaca
    26581 ttcgccaacg ccgcctgtta ctggccgccg ttgagttgcg caccaccgag cgtccgattt
    26641 ttgatatcgc aatggacctg ggttatgtct cgcagcagac cttctcccgc gttttccgtc
    26701 ggcagtttga tcgcactccc agcgattatc gccaccgcct gtagttttat tgcccgcgcg
    26761 ttaactcccg cgcgggtaat tgctccatcc attgcataaa cacctgcggc ggcatcgcct
    26821 tcgcaaaaaa ccatccctgg caatagcgca cgccgcgttt gcgcagccag ttaacctgct
    26881 cctcagtttc gacgccttca gcgatcgttt ttaaccccag gctgtgcgcc agctcgatga
    26941 tgtgttccgc aatcaaatga ctggttttgt gggtggtcag cgtttcaaca aacgatttat
    27001 cgattttcaa aatatcgaca ttcaatgatt taaggttatg caagttagag tagccaatac
    27061 caaaatcatc aattgccact tcgtaaccag cctggcggaa agccagaatt aatcggcgtc
    27121 attttttcga catcaagaaa tgcatgctca gtcacttcaa atttaatttg ctgcggacgc
    27181 accgcgtatt gctctgtttt ctgattgatt cgcgctatca accgtgacgt atgaaaatcg
    27241 gaggccgaca ggttaataga aacatagcga tcggcatgtg ttgccaggta cgcgcccaga
    27301 tcgcggaaga cattatcaat aacataatca gttatctgtt ctatcatccc ctccttttct
    27361 gccagcggaa taaactctgc cggattcatt atttgcccct gctcaccagg ccaacgtaac
    27421 aacgcttcag cgccgataca tttttctgtt ttgatatcga ttattggctg gtaataaaga
    27481 caaagttgat gtttttcgag ggcgcgttgc aatttacgtt tgggagataa atagttttgt
    27541 cgaatacgta gccagagtaa tagcagaaca agactcccca aaataccggc gggcaacgca
    27601 aaaataagat gattataaaa atgggttata agacgttgat atgaagtcgc aacaatggct
    27661 gcaattgggc gttttgtcga ataaattgtc gcatataaat agccatttct ttgtacggtt
    27721 aaatccttca aatgaatcag cggagaaaac gttgctgccg aggcctcttt gcttaacgag
    27781 aaaaaggttt tcatcaccgt atcatacaca ccccattgca atgtcgggtc atcagacatc
    27841 acttcactcc agaagagagg gttgataacc gccacataat ttccccgctg catataggtc
    27901 attttatagc cagagaaaaa aggcgtatcg cggtaataat agatagaaac gttaggttca
    27961 cgcttataat cggccggtgc aatcgtatag ccgtttacag gcgctatcag cgatgagcat
    28021 aaaaaatggt tatcacgaat tcattaatat aaagatagcc acgaataata ttcaacattc
    28081 gcttttgatg ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta
    28141 aatctgcctg gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga
    28201 gctgattatt cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg
    28261 gaaagagaac taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac
    28321 tcattgctat caatttcctg cctgtttata cccggccatt gcaaggtgaa accctggcta
    28381 ccgacgtctt tagataaatc ttacggtata aatagcactt ttgcctgatt tgaaattgat
    28441 acctttttaa tcgtcacctg gatgaaaata attcattgat attcaaaatt aattttctac
    28501 aggaatacag aggcggcggg aagatatgcc cttaatgcca ctgaaaaacc acataatttg
    28561 tacagcaata aaagtcacgg cctaatatgg ctaacaaaca atcaattgtg atatagttca
    28621 caaaattaat gaaacaaaca gagtgtttca tttttgtgtt ctgcaagccc cgcgatagcc
    28681 agagtgtacc acgcctcccg tgaacaacgc cgcgctgtcc agggcatcgg ctcttttcag
    28741 aggaattgtt tgatggctac cctcaccact ggcgtggttc ttcttcgctg gcaacttctt
    28801 agtgccgtaa tgatgtttct ggccagcaca ctcaacatcc gttttcgtcg gtctgattat
    28861 gtcgggcttg cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg
    28921 gggttgcttg gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg
    28981 atgatagaag agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta
    29041 gaa
//
LOCUS       DAJTSA010000046        28914 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000046 DAJTSA010000000
VERSION     DAJTSA010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.046"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO369_002162"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO369_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR5982432.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATTESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="OO369_002163"
     CDS             complement(2138..2584)
                     /locus_tag="OO369_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR5982433.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="OO369_002164"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="OO369_002164"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR5982434.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO369_002165"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO369_002165"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR5982435.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="OO369_002166"
     CDS             complement(5347..6009)
                     /locus_tag="OO369_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR5982436.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="OO369_002167"
     CDS             6182..6595
                     /locus_tag="OO369_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR5982437.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO369_002168"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO369_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR5982438.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO369_002169"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO369_002169"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR5982439.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="OO369_002170"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="OO369_002170"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR5982440.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO369_002171"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO369_002171"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR5982441.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO369_002172"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO369_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR5982442.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="OO369_002173"
     tRNA            complement(9861..9948)
                     /locus_tag="OO369_002173"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="OO369_002174"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="OO369_002174"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR5982443.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="OO369_002175"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="OO369_002175"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR5982444.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWCGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="OO369_002176"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="OO369_002176"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR5982445.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /locus_tag="OO369_002177"
     CDS             14005..14592
                     /locus_tag="OO369_002177"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR5982446.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="OO369_002178"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="OO369_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR5982447.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="OO369_002179"
     CDS             14984..15841
                     /locus_tag="OO369_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR5982448.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="OO369_002180"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="OO369_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR5982449.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="OO369_002181"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="OO369_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5982450.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="OO369_002182"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="OO369_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR5982451.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="OO369_002183"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="OO369_002183"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR5982452.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22430)
                     /gene="etk"
                     /locus_tag="OO369_002184"
                     /pseudo
     CDS             complement(20264..22430)
                     /gene="etk"
                     /locus_tag="OO369_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22450..22896)
                     /gene="etp"
                     /locus_tag="OO369_002185"
                     /gene_synonym="yccY"
     CDS             complement(22450..22896)
                     /gene="etp"
                     /locus_tag="OO369_002185"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR5982453.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22884..24023)
                     /locus_tag="OO369_002186"
     CDS             complement(22884..24023)
                     /locus_tag="OO369_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR5982454.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24069..26165)
                     /locus_tag="OO369_002187"
     CDS             complement(24069..26165)
                     /locus_tag="OO369_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5982455.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26165..26911)
                     /locus_tag="OO369_002188"
     CDS             complement(26165..26911)
                     /locus_tag="OO369_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5982456.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26908..27552)
                     /locus_tag="OO369_002189"
     CDS             complement(26908..27552)
                     /locus_tag="OO369_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5982457.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27659..27964)
                     /locus_tag="OO369_002190"
     CDS             complement(27659..27964)
                     /locus_tag="OO369_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5982458.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="OO369_002191"
     CDS             complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="OO369_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR5982459.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         7002 a         7319 c         7601 g         6992 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggttggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgcctg ggcctgttct gattcagttg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcgggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggtgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacacg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtt accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgcag cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc catccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaaca aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg tgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gtctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga tcagcgtgaa taacgcggtc
    22321 acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc
    22381 tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat
    22441 aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatact
    22501 gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc
    22561 cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg
    22621 agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt
    22681 gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc
    22741 tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc
    22801 cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca
    22861 aaccactagg attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct
    22921 gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga
    22981 gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg
    23041 agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc
    23101 tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc
    23161 ataccctcag cgttacccag cgcttctgcc agggtcatac cgctgcggtc cattttcagc
    23221 gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata
    23281 aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc
    23341 gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct
    23401 gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg
    23461 gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg
    23521 aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta
    23581 atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg
    23641 tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc
    23701 cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc
    23761 ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata
    23821 atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt
    23881 ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa
    23941 ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa
    24001 aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt
    24061 acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggatc gagctggtat
    24121 ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg
    24181 ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa acctttacta
    24241 aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc
    24301 gccacgccgc tgtcaaagag tttggcgacg tcgatcgttg cgcctttatc ttttgccaga
    24361 tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc
    24421 gtcacgaaac ctgtgggcgt ggaataatcg gtgaagcgca tcatgttgtc ccagtcacgc
    24481 tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaaga
    24541 agctcggaac cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag
    24601 aaaccattgc ctaagtcggc aaagtaattc gcttgcaagt tgttgagata aacgtcattg
    24661 cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggtaacagt
    24721 gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgtc atcaagcagc
    24781 aggtggtcgg taaaccagta gcgggcgctg gacatcagcc ccagctggaa catatagaaa
    24841 tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct
    24901 tcacgaatac ggtagcaccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt
    24961 tgcagttgct ctgatttacc aggcgctgtg ccggccagct gtttgcgcag gctggctacg
    25021 tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca
    25081 acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagag
    25141 tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag ctgaatttct
    25201 ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac
    25261 tgcaaacctt cagactctgg cgcaggctgc caggccggtt ttggcgtatc gcgtagggca
    25321 gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt gtttccgcgt
    25381 tcataactga ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc gacgttgaaa
    25441 tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc gtcgtattcg
    25501 agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc
    25561 gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga
    25621 caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag
    25681 ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatat
    25741 tcaccgtcaa acaaacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc
    25801 cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga
    25861 tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg
    25921 gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta
    25981 tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac
    26041 aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact
    26101 tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc
    26161 ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat
    26221 atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt
    26281 ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa
    26341 tcaccataac gttattcttg tctgcgcctg caaggcgggg gtgatcttgc agataatcgg
    26401 taacgctacg gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag
    26461 taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc
    26521 tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat
    26581 tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt
    26641 ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt
    26701 tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt
    26761 gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgctag
    26821 gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc
    26881 tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac
    26941 gggaataacc cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc
    27001 gacccagaat gtgttttgcc actgtttttc tggcagcgtg gaagtcacct cttcctgcca
    27061 gacattacac agcacattgt gacccgcaat attcagcgtc tctaatccag caaaggaaaa
    27121 agtagaactt agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt
    27181 ccagcggctg ccatctttta ttgccagcgc gttacgcaga ggggcccgcc cgggatttgt
    27241 cacttccagc agattattat taagtttgac ggtctttaaa agacgtccat tgtgggtaac
    27301 cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac
    27361 cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg
    27421 gatctgctga gccgctacag tgatatcctg attatcgaaa aggctggcgc gaaatgtatc
    27481 gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat
    27541 aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc
    27601 ggtacgaata tcgcaaccgg gtattattta acttgaattt cgacgcgttt aagcagaatt
    27661 agcgcgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg
    27721 ctgcaactac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg
    27781 tgccgacagc gccagcagcc acgccactag tggtagccgc agtcgtggtt gcacctgtag
    27841 tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct gcttcagggg
    27901 cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt
    27961 tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct
    28021 actttgagta taacatcctt gtgaaatctc cctttcctca taccgctagc actatatact
    28081 gcgggtggga aaggcgcgca gagggaaata taagattgtt aacgatttcc ctctggaaat
    28141 acgacttgtg agcataagat aaatcttaga aaaatacgaa ttaaagataa gaaaattgtt
    28201 acgaaagcta ttaattattg ttggtaatat agtttcaagt ggtactataa ctggctgtta
    28261 aatggcattt ataactatta ggtgcagaga tattcgctta aatggattag tttcttagtg
    28321 gaaaagtaac tgatgttata atgtatggtg ttgactattg aactattcca ataaaaccag
    28381 tcagctttaa acaagcagcg tcatattaag agagataaac atttgccgct gttggtcctc
    28441 gcaggccatt tacgcggcaa aattccacgc gtaatcctgg tataagcact tctgcgtcgc
    28501 ggggagtgaa tgcggaaata tggacctgga cttctttacg accgtcggag gggataatga
    28561 atcctttgcc gcttttgcga tcaaaggttt tgacaattcc tgtcatttta cgggacaaac
    28621 aaattcctta ctgaaaatac tgcgctgcac tatacggggt taataaaata aagccagcga
    28681 tatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat catgccttaa
    28741 tagctgcgtc ggtttgaaag acagacagca tacaaagtag tttactaaag cagttctcat
    28801 tatcaggcat tatccccttc ttttgagtct ctctcctgaa cactaagtgg tttctgtatt
    28861 aaagccctgt ttgccgaaag gcccaaaatg aaggaagtaa aatatgtcta ataa
//
LOCUS       DAJTSA010000047        28668 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000047 DAJTSA010000000
VERSION     DAJTSA010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.047"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..648
                     /locus_tag="OO369_002192"
                     /pseudo
     CDS             1..648
                     /locus_tag="OO369_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163825.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            645..1031
                     /locus_tag="OO369_002193"
     CDS             645..1031
                     /locus_tag="OO369_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5982460.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            1056..3269
                     /gene="tssI"
                     /locus_tag="OO369_002194"
     CDS             1056..3269
                     /gene="tssI"
                     /locus_tag="OO369_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR5982461.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            3292..3736
                     /locus_tag="OO369_002195"
                     /pseudo
     CDS             3292..3736
                     /locus_tag="OO369_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3758..4165
                     /locus_tag="OO369_002196"
     CDS             3758..4165
                     /locus_tag="OO369_002196"
                     /inference="COORDINATES: protein motif:HMM:NF017315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HCR5982462.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            4162..6450
                     /locus_tag="OO369_002197"
     CDS             4162..6450
                     /locus_tag="OO369_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5982463.1"
                     /translation="MIEDGSPNVFLGGGTQTVLEISPEIPDWLRQVVDVLYVVAGMLG
                     GLAGAWRQAAKMGSKFGTKCAAKFIGGEMVGMAISDTVMGLFSNPVDVTTGQKILLPE
                     TDFTLPGRLPVTCSRFYASHLETEGLLGRGWRLNWEINLREDETYITFIGVQGRELSY
                     PKEMLIPGHQIFDPEEQFYLSRLHDGCYVLHYTDCSYYVFDEFDDHGVAPLLFMETPY
                     RQRIAFGRENGRLVRVASSSGHHLLLHRTMTQAGERLSHIELLKGGRPGNLVEYRYDD
                     NGQLTGVVNRAGVTVRQFAYENGLMTEHRNATGFTCTYHWEEIEGFPRVVEHTTSDGE
                     DYRFHYDFAGGQTVVTGRPEQKWQWWFDEETYVTAHRTPGGGMYRFTYNENHFPVAVE
                     LPGERRVTLEYDTLSRVVKETDPAGRVTQTQWNGSFAEITRRALDDDHVWKADYNEHG
                     QVIRETDPEGRVTRYGYDDQGLPETVCHPGKQQDRYTWNALGLLSSHRRITGSVQSWQ
                     YTQRGMLARHTDEEKRETRWQYTPEGLVASLSNGNGAQYRFSYDGDGRLTGEQRPDGL
                     IRMFALNADGFPVIIPTQGTEGGVRNEQQERDALGRLLRSDTQHSTRTFSYNRLDQIT
                     EVTLTPTEEGERLHHMQADTVRFAYDRSGWLTAEHSVHGSIKYRRDALGNPTDITLPD
                     GQHLSHLYYGSGHLLQTALDGITVSEYERDSLHRQVIRTQGKLATFSGYNADNRLSWQ
                     RSLPGGSQNPQQAVLPRRRTTA"
     gene            6447..7436
                     /locus_tag="OO369_002198"
     CDS             6447..7436
                     /locus_tag="OO369_002198"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5982464.1"
                     /translation="MTARDYIWNADGEVGGINDKLRGCLVFSYDRSGWLTSRTGQMYD
                     HDHYYYDKAGNLLTDEYQGAVMDNRLPGYGRDRYRYNEWGELTERRDQQLEWNAQGQL
                     TRVISSNSETRYQYDALGRRISKATSNLHTDRGERSRTTFVWEGFRLLQETTWQGKRT
                     YLYDAEQPYTPVAAITGRGESQKIWYYHTDLTGTVHEVTAPDGTLVWAGYQAGFGENR
                     GDISNSGAYFEQPQRLPGQYFDEETGLHYNLFRYYAPECGRFVSQDPIGLNGGLNLYA
                     YAPNPLGWIDPLGLNSLGIDSNNIFRGDGNYKGGGIGQPKSGTISAQDIIDHL"
     gene            7536..7739
                     /locus_tag="OO369_002199"
     CDS             7536..7739
                     /locus_tag="OO369_002199"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5982465.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7757..8077
                     /locus_tag="OO369_002200"
     CDS             7757..8077
                     /locus_tag="OO369_002200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5982466.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(8143..8375)
                     /locus_tag="OO369_002201"
                     /pseudo
     CDS             complement(8143..8375)
                     /locus_tag="OO369_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(8485..9255)
                     /gene="yafV"
                     /locus_tag="OO369_002202"
     CDS             complement(8485..9255)
                     /gene="yafV"
                     /locus_tag="OO369_002202"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR5982467.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYGAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            9436..9882
                     /gene="ivy"
                     /locus_tag="OO369_002203"
     CDS             9436..9882
                     /gene="ivy"
                     /locus_tag="OO369_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR5982468.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9925..12369)
                     /gene="fadE"
                     /locus_tag="OO369_002204"
     CDS             complement(9925..12369)
                     /gene="fadE"
                     /locus_tag="OO369_002204"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR5982469.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12609..13187
                     /gene="lpcA"
                     /locus_tag="OO369_002205"
     CDS             12609..13187
                     /gene="lpcA"
                     /locus_tag="OO369_002205"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR5982470.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13393..14160
                     /locus_tag="OO369_002206"
     CDS             13393..14160
                     /locus_tag="OO369_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR5982471.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(14131..14871)
                     /locus_tag="OO369_002207"
     CDS             complement(14131..14871)
                     /locus_tag="OO369_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR5982472.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            15255..15776
                     /locus_tag="OO369_002208"
                     /pseudo
     CDS             15255..15776
                     /locus_tag="OO369_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            15952..16449
                     /gene="rayT"
                     /locus_tag="OO369_002209"
     CDS             15952..16449
                     /gene="rayT"
                     /locus_tag="OO369_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR5982473.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(16666..18378)
                     /locus_tag="OO369_002210"
                     /pseudo
     CDS             complement(16666..18378)
                     /locus_tag="OO369_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            18350..19135
                     /gene="lafU"
                     /locus_tag="OO369_002211"
     CDS             18350..19135
                     /gene="lafU"
                     /locus_tag="OO369_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR5982474.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            19206..20261
                     /gene="dinB"
                     /locus_tag="OO369_002212"
     CDS             19206..20261
                     /gene="dinB"
                     /locus_tag="OO369_002212"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR5982475.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            20313..20606
                     /gene="yafN"
                     /locus_tag="OO369_002213"
     CDS             20313..20606
                     /gene="yafN"
                     /locus_tag="OO369_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCR5982476.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            20609..21007
                     /gene="yafO"
                     /locus_tag="OO369_002214"
     CDS             20609..21007
                     /gene="yafO"
                     /locus_tag="OO369_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCR5982477.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            21017..21469
                     /locus_tag="OO369_002215"
     CDS             21017..21469
                     /locus_tag="OO369_002215"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5982478.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            21715..22758
                     /locus_tag="OO369_002216"
     CDS             21715..22758
                     /locus_tag="OO369_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR5982479.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            22820..23434
                     /locus_tag="OO369_002217"
                     /pseudo
     CDS             22820..23434
                     /locus_tag="OO369_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(23491..24948)
                     /gene="pepD"
                     /locus_tag="OO369_002218"
     CDS             complement(23491..24948)
                     /gene="pepD"
                     /locus_tag="OO369_002218"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5982480.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            25210..25668
                     /gene="gpt"
                     /locus_tag="OO369_002219"
     CDS             25210..25668
                     /gene="gpt"
                     /locus_tag="OO369_002219"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5982481.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            25760..27004
                     /gene="frsA"
                     /locus_tag="OO369_002220"
     CDS             25760..27004
                     /gene="frsA"
                     /locus_tag="OO369_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5982482.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            27062..27463
                     /gene="crl"
                     /locus_tag="OO369_002221"
     CDS             27062..27463
                     /gene="crl"
                     /locus_tag="OO369_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5982483.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(27502..28557)
                     /gene="phoE"
                     /locus_tag="OO369_002222"
     CDS             complement(27502..28557)
                     /gene="phoE"
                     /locus_tag="OO369_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR5982484.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         7080 a         7127 c         7606 g         6855 t
ORIGIN      
        1 acaaatattg atggcaatat cattatacat aaggatggta ggaggacgta tttattaaat
       61 caaaatactg gttcgattac ggctgtgata agatggaagt atgagtagta taaggataat
      121 accgttacag atgactggac tatatgggaa aaagctattt ttatgcgagc gatatattta
      181 tctgttcagc aggcatggaa tgggaaaata acttattctg tttccggtga atctgagttc
      241 gctaaaaaat ttcagggtaa agctttgcct tttgatgtaa gaataatttc tgcatctcag
      301 aatgaagact ggttggttat tgcaacaaag gttttgcctg gtgctgattt aagaacatat
      361 gttgatttta aaaacagtac ggttcatgtt gattcagcgg gcctggaaaa agtagcgaaa
      421 tgtattaact gtaataatac gttgcaagta aatatacctc atgaagccgg acatgtactt
      481 ggttacttag atgatgatta tgattcttcg tctccgtatg ttggagatat tagtgggttg
      541 atgaatgtgg gaatggagct ttgggaacga taccttaaaa acgcaactat aacgttgaat
      601 atcataatgc ctgaaactaa atttacttta ttgaatgtaa ccaaatgaaa tttttctgta
      661 ttatagtgat tttcctttca ataaattaca tagctatagt gaatgttttc aagtcttctg
      721 cagttgctca atccaccaaa tattatgaag aaaatgatat acatgatgga agtaatgaca
      781 tcttttttat gaagaaatat gggccgcctg ctcatgagtt cgtgaggcca cgatataaat
      841 ttgttggtga gtattatatc ggcttgaatc aaacttatcg cataacagat cctgaatttc
      901 aagatgttcc tattaaggaa atgttctgga atttgcaggg ggatttgaat ctgacatgtt
      961 ggtttcatta taaagacggg caatggagag tgattactta tattttttgg ccaccagggg
     1021 ctagatttta atccggtatt aaaggagcaa atatcatgag ttttgtatct acgggtaata
     1081 aaccagttgg aaacggtgga cccgtcatca ccacaccacc aatagctggc gaaagtggcg
     1141 gtatgagcac aggttcagct gtaaccgatg tatctggtgc cgccgaggag atggcagaac
     1201 aggctgcggc ggacctgttt ggtgcgctgc cggaaccgtc aggtctggtt aaagccgcag
     1261 tagctgctgc gcaggctgcc gctgcagcag cagggatctc ggatatggct ggtgcagttc
     1321 aggatgcagc ggcgtcgctt gcagccggtg ctccgggggc gcataacgtc acagtaagtg
     1381 gtagtgcggt gccgccgcag atgcttttgt ttgcaggcat gaatggttcg gaagggcttg
     1441 ggaatctgtt ttcttacaca gtacagctta aaacgctgga cgccctgaac ctggggtacg
     1501 tttcccccgc tgctaacctg ccgcttcagc tcatggtggg caaagacctg tgcgtcagta
     1561 tcgaactgga cggcggcgga aaacggtata tcagcgggct ggtgacggcg gcgagggtgg
     1621 tcggccatga gagccgttcg gtaacgtatg aattacgcat tgagccatgg ctgaagctgc
     1681 tgacccatac cagcgactac aaagcgttcc agaacaaaac cgtggtggat attctcgatg
     1741 aagtactgga tgaatattcc tttccggtgg aaaagcggct ggtggaaaat tatccacccc
     1801 gcgcctggca ggtgcagtat ggtgaaaccg acttcgattt catccagcgc ctgatgcagg
     1861 aatgggggat ttactggtgg tttgagcaca gcgagaacag ccacacgctg gtgctggtgg
     1921 atgccatcaa tggtcataaa gcgtgccctg actcgccgct ggtggagtgg caccaggaag
     1981 ggctgaaact ggataaggaa tttatccaca ccattacggc cagtgagagg ctgcgtactg
     2041 gaaagtgggt gatggacgat tttgatttta tgaagcctcg ttcgctgctt aaaagtaccg
     2101 tggccagtcc gcgtgatacc ggtcatgctg aatacgagca ttacgaatgg cctggtgatt
     2161 acttcactac gggcgaaggc gagatgctga cgcgcattcg tatggaagcg cagcgcagcc
     2221 caggcagccg ggcacatggg gccggtcata tccgtaccct catgaccggg tacaccttta
     2281 cgttgatgaa tcacccgacg gcagaaatta atcaggaata tctgctggta cagaccactc
     2341 tgtttttacg ggataatgcg cagcacagcg gtcaggatca gcattttacc tatgtcacca
     2401 cgtttgaact gcaccccacc agcgaagttt accgcccaca acgaaccctg agcaaacccc
     2461 acaccaaagg cccgcagagc gccatcgtca ccggcccggc ggggcaggaa atctggacgg
     2521 ataaatatgg ccgggtgaag gtgcagtttg gctgggaccg ttacggaaag aatgacgaaa
     2581 acagttcctg ctggatccgc gtcagctatc cgtgggcggg taaaggattc ggcatgatcc
     2641 agatcccgcg tattggccag gaagtactgg tggatttcaa aaacggcgat ccggatttgc
     2701 ccatcatcgt ggggcgcacc tataaccagg acaccatgcc gccgtggggg ctgccgggca
     2761 tggcgtcgca gagtgggata ttcagccact cattgcaggg agggccgact aacggaaata
     2821 tgctgcgctt cgatgataaa acgggtgcgg aagaggtgaa gttccatgcg gaaaaagacc
     2881 tcaataccac ggtaaaaaac aatgaaaccc acacggtcat ggtcgatcgc ataaaaacca
     2941 ttgtcaaaaa tgaaaccatc agcgtgggag aagaccgtaa tgccaccatc acaaaaaatg
     3001 atggtctttc cgtcaaactg gctcaaacca tcaatgtcgg aacgacatat cgtctggatg
     3061 ttggcgatca atttaccctt cgttgcggca atgcagcgct tgtattacac aaggatggct
     3121 cgattgagtt ttgcggaaaa caactgttac tgcacaccag cgatgttatg caactcattg
     3181 gcagtggcat tgatatgaat ccggatggcg gcacagccat gactgcggat gatattgccc
     3241 cgttacctac ccccgacgtt tctgaataaa aaatataaaa ggaatttttc tatgaccagc
     3301 ctttaccgta ttcaggaggg ctgctttgcc ctgcctgaaa cttttcttga tcgcaccgtc
     3361 aatatcttcg tcccatcggg caatgaaagg gcaactccca gtctgaatat tttccgcgat
     3421 acgctcaggc cagatgaaaa tctgaccacc tatattgacc gccagatagc cttgatgaaa
     3481 aaaaaaccta ggccagcacc gggtactttc gcggggaacg gcactggcgg gaactggcga
     3541 agctgccatg acaggtgaac agattctcgc aactcatcgt tctggaaaaa cagaagtgta
     3601 ccagcgtcag gccgggttta tcaccgggcc agcaaaggta ctaatgttga cgctgaccac
     3661 tcagcgtcct tttgatgatc acaccgacca gctatggact gcatggttga ctagttttca
     3721 gcctgctaaa agctaaaaaa aacagggagg ttttgtcatg tatgaagccg cacgggtgga
     3781 tgaccccatc taccacacca gcgcccttgc cgggtttttg ataggggcca tcataggtat
     3841 cgccattatt gccgtcgcca gctttgcttt ttttacctgc ggctttttgg ccgggctgat
     3901 tttgagtttt ctggctgatc agatagcttc cggagtcctg caactgggtg aggctattgg
     3961 gcgctcgata cattccacag tgggaaaaat tctgaccggt tctgataatg tcagcaccaa
     4021 cagtcgcccg gccgcccggg ccgtactgag tacggtgata tgcgaagacc attccccgga
     4081 aatccgcatt gcccaaggtt ccggcaacat ctacatcaac agccagctgc ccgtaaggat
     4141 gaccacactg agtgtgatgc agtgattgag gacggctccc ccaatgtctt tctcggcggc
     4201 ggtacgcaga cggtactgga aatcagcccg gaaatccctg actggctgcg tcaggtggtg
     4261 gatgtgctgt atgtggtcgc cggaatgctt ggcgggctgg ctggcgcctg gcgtcaggcg
     4321 gcaaagatgg ggtcgaaatt tggtacaaaa tgtgcggcaa aatttattgg tggcgaaatg
     4381 gtcgggatgg ccatctctga cacagtcatg ggattgttca gcaatccggt agatgtcacc
     4441 actgggcaaa aaattttgct gccggagacg gattttaccc tgccaggacg cctgcctgtc
     4501 acctgttcgc gcttttacgc cagccacctg gaaactgaag gattgctggg cagaggatgg
     4561 cggctgaact gggagataaa cctgcgtgag gatgaaacat atatcacctt catcggtgta
     4621 cagggtcggg aactgagtta tccgaaagag atgctgatac cgggccacca gatattcgac
     4681 ccggaagagc agttttacct gagccgtctg catgacggat gctatgtgct gcattacacc
     4741 gactgcagct attacgtgtt tgatgaattt gatgaccacg gcgtcgcgcc gttacttttt
     4801 atggagacgc cgtaccggca gcgtattgct ttcgggcgtg agaacggcag gctggtgcgg
     4861 gtggcctcca gcagcggaca ccatctgtta ttgcaccgca cgatgacgca agcaggggaa
     4921 cgcctgtcgc atattgaact tttgaagggc gggcgtcccg gtaacctggt ggaatatcgt
     4981 tacgacgata acgggcaact gactggcgtg gtgaaccggg ctggtgtgac ggtgcgccag
     5041 tttgcctatg aaaatggcct gatgacagag catcgcaacg cgacgggctt tacctgtacc
     5101 taccactggg aagagattga aggtttcccg cgcgtggtgg aacacacgac cagcgacggc
     5161 gaggattacc ggttccacta tgattttgct ggcgggcaga cggtggtaac cggcaggccg
     5221 gagcagaagt ggcagtggtg gtttgatgaa gagacgtatg tgactgcgca ccggacgccg
     5281 ggcggcggaa tgtaccgctt cacgtacaac gaaaaccatt ttccggtagc ggtggaactc
     5341 cccggcgaac gcagggtaac gctggaatac gacaccctga gccgggtggt gaaagagacg
     5401 gacccggcag gccgggtgac acagacgcag tggaacggca gttttgctga aatcacgcgc
     5461 cgggcgctgg atgacgacca cgtatggaag gcggattaca acgaacacgg ccaggtgata
     5521 cgggagacgg acccggaagg gcgcgtcacg cggtacgggt atgatgatca ggggctgccg
     5581 gagacggtct gccaccccgg taaacagcag gaccgttaca cctggaacgc gctggggtta
     5641 ctgagcagcc accgtcgcat cacgggcagt gtgcagtcct ggcagtatac gcagcgcggg
     5701 atgctggcgc gccatacgga tgaagagaaa cgggagacgc gctggcagta caccccggaa
     5761 gggctggttg ccagtctgag caacggcaac ggggctcagt accggttcag ctacgacggc
     5821 gacggacggc tgacgggaga acagcgcccg gacggcctga taaggatgtt tgcgctgaat
     5881 gcggacgggt ttccggtcat catcccgacg cagggcacag agggcggggt gcgcaatgaa
     5941 cagcaggagc gtgatgcgct gggccgcctg ctgcgcagtg acacgcagca cagtacacgg
     6001 accttcagct acaaccgtct tgaccagata acggaagtga cgctcacccc cacggaagaa
     6061 ggggaacggc tgcaccacat gcaggcggac acggtgcgtt ttgcgtatga ccgcagcggg
     6121 tggctgacgg cagagcacag cgtgcacggc agtatcaaat accggcgcga tgcgctgggc
     6181 aatcccaccg atatcacgtt gccggacggg cagcatctca gtcatcttta ctacggcagc
     6241 gggcatctgc tccagacggc actggacggt atcacggtca gtgaatatga gcgtgacagc
     6301 ctgcaccgtc aggtgatacg cacgcagggg aaactggcga cgttcagcgg ctacaacgcc
     6361 gacaaccggt taagctggca gcgcagcctg ccaggtggca gccagaatcc acagcaggcg
     6421 gtactccccc ggcgcagaac gacagcgtga cggcgcggga ctatatctgg aacgcagacg
     6481 gtgaagtcgg cggcatcaat gacaaactgc gcggttgcct ggtgttcagc tatgaccgca
     6541 gcggctggct gaccagccgc acggggcaga tgtatgacca tgaccattat tattacgaca
     6601 aagcgggcaa cctgctgacg gatgagtatc agggagcggt gatggataac cgcctgccgg
     6661 gttacgggcg tgaccgctac cgctataacg aatggggcga actgacagag cgcagggacc
     6721 agcagctgga gtggaacgcg cagggccagt taacgcgggt catcagcagc aacagcgaga
     6781 cgcggtatca gtacgatgcg ctgggcaggc gcatcagcaa ggcgaccagc aatctccata
     6841 ccgaccgggg agaacgaagc cggacaacgt ttgtgtggga agggttccgg ctgttacagg
     6901 agacaacgtg gcagggaaag cggacatacc tgtacgatgc ggaacagccg tacacgccgg
     6961 tggctgccat cacggggcgc ggagagagcc agaaaatctg gtattaccac acggacctga
     7021 cgggcacggt acacgaggta accgcgccgg acgggacgct ggtgtgggcg ggctaccagg
     7081 cgggatttgg tgaaaaccgg ggcgacatca gcaacagcgg ggcgtacttt gagcagccgc
     7141 agcggctgcc ggggcagtac tttgacgaag aaacagggct gcattacaat ctgttcaggt
     7201 attatgcgcc ggagtgcggg aggttcgtaa gccaggaccc gataggatta aacggtgggt
     7261 tgaacttata tgcttatgcg cctaatccgt taggttggat cgatccgcta ggactgaatt
     7321 ctttaggaat agattcaaat aatatattta gaggagatgg taactataaa ggtggcggaa
     7381 ttggacagcc aaagagtggc acaatatcag cacaggacat tattgatcat ctctaagaaa
     7441 aaggtgatgg gcgtttaaca tcttttagta ctaaaaaaac atctagttcc aactctcgtg
     7501 gtgcagattt tttctcaaaa aaccatgtct tcaaagtggc agtttctgat ctcgatgatt
     7561 tagttaaaaa taatcagatc actgttttaa cacaagaaaa agtaaaagca atatttggtt
     7621 tagaatcaag caagaaaatt agaaaagaac ttggtaatat tcttcagaat atggctagga
     7681 ataatgaggt gttagttcat gggcaaattc ttggaaataa agttagccga tgtaattagt
     7741 tagtggagag gaagttgtgg acgaaattag aataagaatt gctggtaata cggctgcgcc
     7801 ttgtttccat gtgctaaaaa ataagggata taaagtaaca atggaaactc atgcattatc
     7861 tgacgattgt ttagattgtg tatatgattg gtatgcggaa aaaaatggac aattttttag
     7921 tgcaacgagt ccagaagagt tacttggatt agttgcaatg tgggaagttc gtggtaatga
     7981 ctggagagct aatttgaatg agataaatac ttatcaaaaa gccatagaag aatccccgtt
     8041 ttttgataaa aacggcaatg aaatagagtc tgactaaatt ttattgatat agcaacgata
     8101 ataaccggaa atttcttcca gatctttccg gctttttatt atttaacttt cgttcagcac
     8161 actaaacatc ccgtctcgca tccccttaac tggctgttta acctgataaa gttccctgcg
     8221 cagttcctgt acttcgttgt tatcccaagc aggatcaaac atccctccga gatcttcacg
     8281 gtaacgcccg tgccgatctc aaatcccgct tttctcagcc atttggcgct gattttgatc
     8341 accggcgtgt cgctgcggtt gggcagataa cccacggtac agcgacgctg tgttttggaa
     8401 atggtgactt ctggcgtaat attgtgttta agttaggggc tttcgtaatc tagcccagcc
     8461 cccggcccta aacacaaccc ccactcacca caacctaaac tcatccgcat cccgccatgc
     8521 cggaaacttt tctctatatt cccgcaatgc caccatcgac agctccgcat caatacgcgt
     8581 tgccttatgc gcgtcggcgg tagcgataat ctcgccttgc ggattaatca cccggctgtc
     8641 accgcgataa tggcagccgt tgccatcgct gccgacgcga ttgcatcccg ccccatacgc
     8701 ctgattctca atcgcgcggg ccgtcagcaa tgcctgccag tgcagagagc gcggagctgg
     8761 ccagttggcg acgtacaggg cgaggtcata atcgttgaga ttgcgcgacc acactggaaa
     8821 acgtaagtcg tagcacacca gcggcaaaat acgccagccg cgccattcca caatcactcg
     8881 cgcattgccc gctttataat gcagatgctc atctgccatg cggaacagat gacgcttatc
     8941 ataaaaatgt accgtgccgc ccggctcaac cagcaaaaag cggttaaccg aaccagactc
     9001 cgtttgtaat gcaacactgc ctgcaatcag cgcattgcac tgctgcgcct tagctgtcat
     9061 ccagttcact acgtcatctt gtgcaagcga cgaagctgcc gcttccatgg caaagccgct
     9121 ggtaaacatc tccggtagaa cgatcacatc gcgcccggta ataccttcca gttgacgatc
     9181 aaaatggcgc aggttggcag gaccatccac ccacaccaac ggttgctgca aaagcgtaat
     9241 cttcaaacta ggcacggtgt acaactcctt tatgcgaagg gttttataac tttaacacct
     9301 tatcaggcag ttgccttagc gcagaataaa ttgataacaa atgctgatat tggaaatatc
     9361 tgatttgcaa attatcgtgt tatcgccagg ctttaggagg ttaataacat gggcaggata
     9421 agctcgggag gaatgatgtt taaggcaata acgacagtcg ccgcactggt catcgccacc
     9481 agtgcaatgg cgcaggatga tttaaccatt agcagccttg caaagggcga aaccaccaaa
     9541 gctgcgttta atcagatggt gcaagggcat aagctacctg catgggtgat gaaaggcggt
     9601 acttatactc ccgcacaaac cgtaacgttg ggagatgaga cgtatcaggt gatgagcgcg
     9661 tgcaaaccgc atgactgtgg ctcgcaacgt atcgctgtga tgtggtccga gaaatctaat
     9721 cagatgacgg ggctgttctc ggctattgat gagaaaacgt cgcaagagaa actcacctgg
     9781 ctgaatgtga acgatgcgct ttcgattgat ggtaaaacgg tgctgttcgc ggcgttgacc
     9841 ggcagcctgg aaaaccatcc ggatggcttt aattttaaat aattagcgga taaagaaacg
     9901 gagcctttcg gctccgttat tcaattacgc ggcttcaact ttccgcactt tctccggcaa
     9961 ctttaccggc ttcgtcgcca gctcttccgg atcaaagtca tcaacgttaa tactgcgcag
    10021 acggctttct tccgctttca ccagaatagc ggcttcatct ttatcaatca gccccttcgt
    10081 cagcgcgttg tgcgccagtt catccagacg ggtaaatggc agatttttac ccagctcttt
    10141 acagatccgc tgatgaatcg ggtcggcggc aatcacatcc accaacgcct cttccagtaa
    10201 accaaccggg ttatgctcgc ttggcgtcag gtactgaccg cgaccaatac gtgaacgagt
    10261 ggcgttcggc acttgtaaaa tcttcgccat tttatgatcc agcttgtcag acggtgccag
    10321 ataatgacgt ccggtcggga agatcaccac attcagcagc ccggcaacca cgcggttcgg
    10381 gaagttttgc agtaaattgt ccattgcctg ttctgcctga tacagcgcat cctgtacgcc
    10441 ccagtgcacc agcggcagat cggcttcatt acggccttcg tcgtcataac gcttcagaac
    10501 ggcagaggcg aggtaaagct ggcttaaaat atcccccaga cgtgccgaga tacgctcacg
    10561 acgtttcagg ctgccgccca gcagtgccat cgagacatca gaaagcaggg cgaggttggc
    10621 gctcaggcgg ttcaggtgct gatagtagcg tttagtggca tcgccggttg gcgtgctgct
    10681 ggttaaaccg cgcgtcaggc ccagccagaa gctgcgaact ttgttgctac cgacgtgacc
    10741 gatatgtttg aacaacagtt tatcgaacgc gttgacgtca ttgttcttcg ccgcttccat
    10801 ctcttccagc acgtacggat ggcaacgaat cgctccttgt ccgaagatca tcatgctgcg
    10861 ggtcagaatg ttagcccctt caacggtgat ggcaatcggt gcgccctggt aagcacgcgc
    10921 caggaagttg ctttgcccga gcataatgcc tttaccgccg gtaatatcca tcgcatcaat
    10981 aatcgactgc tgcccgcggt gggtacagtg atacttaacg atagccgaca gcacggcagg
    11041 tttttcgccg agcataatgc cgtaggtaat cagcgatgcc gcagcatcca ttacataagc
    11101 gttcccggca atacgcgcca gcggctcttc aatcccttcc atcttaccga tagagatttt
    11161 gaactgacgg cgaatgtgag aatacgcgcc cgttgccagc gctaccgatt tcacgccgcc
    11221 ggttgagttg gaaggcaggg tgatgccgcg gcctaccgag aggcactcca ccagcatccg
    11281 ccagccttgc ccggccattt tcggcccgcc gatgatgtaa tcgatcggca cgaagacatc
    11341 tttaccgcgc gtcggtccgt tctggaacgg tacgttcagc gggaagtggc gacgaccaat
    11401 ttccacgccc ggcgtggtgg ttgggatcag cgcacaggta atgcctaaat cttctgcacc
    11461 gccgagtaat ttttccgggt cggagagttt aaacgccagc ccaagcacgg tcgcaatcgg
    11521 tgccagcgta atgtagcgtt tgttccaggt cagacgcatc cccagcacct gctggccctg
    11581 ccattcgccc atgcagacaa tcccggtgtc cggaatcgcg cccgcatcgg aacccgcttc
    11641 cgggctggtc agtgcaaagc aggggatctc ctgaccacgc gccagacgcg gcagatagtg
    11701 atctttctgc tcgtcagtgc cgtaatgttg caacagttcg cccgggccta atgagtttgg
    11761 cacgccgacg gtaatcgcca ggatcccgct cacgccagag agtttttgca gcacacgaga
    11821 ctgggcataa gctgagaact ccagtccgcc gtactctttt ttgataatca tcgcgaagaa
    11881 gcgatgctct ttaaggtacg cccacaactc cggtggcaga tccgccagct catgagtaat
    11941 ctggaaatca ttcgccatcc ggcaggcttc ttctaccggg ccgtcgagaa acgcttgctc
    12001 ttcagcggtc aggcgcggct gcggatagtt atgcagcttt ttccagtccg gcttgccctg
    12061 gaacaagtcg ccctcccacc aggtggtgcc cgcatcaatc gcttctttct cagtgcgcga
    12121 catcggcggc atcaccttac ggaaaccgcg aaataccggc gcggaaatca tcgacttacg
    12181 cataggcgca aagttaaatg gcacgaggat aatggtcaga ggcaccagta cccacgccga
    12241 ccacagacca gcaacgccga gggcggctgt ccaggcgagc aaaatcagac tgctgataaa
    12301 taagctcacg cggtgataga acaacgcgcc gagcaggaca accgtagcga gaatactcaa
    12361 aatcatcata acgaaaagcc ccttacttgc aggaggtctg accacttgtg atgatatggt
    12421 tgtagtggat gtaaaaacat ttagcaatgt gtttacaata taattacaac aaagctcaca
    12481 ttgttgctgt ttttatccgc acttcaggtc aaaaagtcct ggtcatagca cctgcccgta
    12541 cttctcgctt ttggcggtat ccggtacact gcattttgtc tattacattt atgctgaagg
    12601 atatcctcat gtaccaggat cttattcgta acgaactgaa cgaagcggcg gaaacgctgg
    12661 ctaacttttt aaaagatgac gccaatattc acgccattca gcgcgcggcg gtcctgttag
    12721 cagacagctt taaagccggt ggcaaagtgc tttcctgcgg caacggcggt tcccattgcg
    12781 acgctatgca ctttgccgaa gagttgaccg gtcgctaccg tgaaaaccgt ccgggctacc
    12841 cggcgattgc tatttctgac gttagtcata tttcctgcgt cggtaatgat ttcggtttca
    12901 atgatatttt ctcccgctac gttgaagcgg taggtcgcga aggcgatgta ctgctgggga
    12961 tctccacctc cggtaactct gcaaacgtga tcaaagcgat cgcagcggcg cgtgagaagg
    13021 ggatgaaagt gatcaccctg accggtaaag acggcggtaa aatggctggc acggcggata
    13081 tcgaaattcg cgttccgcac tttggttatg ccgaccgcat tcaggagatt cacattaaag
    13141 tgatccatat cctgatccag ttaattgaaa aagagatggt taagtaagtc tggcgtaggc
    13201 cggataaggc gtttacgccg catccggcac ttgtgcgctg atgcctgatg cgatgctgac
    13261 gcgtcttatc atgcctacaa atctgtaccc gaaccgtagg ccgaataatt cgtttacgcc
    13321 gcatccgacc tgaaaattct taaatcaatc ttcgccgggg tccatgcgct cccgctgttg
    13381 tggaggttac ccatgtgcga attgctcggg atgagcgcca acgtccctac cgatatctgc
    13441 tttagtttca ccggacttgt acagcgtggt ggtggaaccg ggccacataa agatggctgg
    13501 ggcattacct tttacgaagg taaaggctgt cgcacattta aagatccaca acccagcttt
    13561 aattccccca tcgccaaact tgtccaggac tacccgataa aatcctgttc ggtggtggct
    13621 catattcgcc aggctaatcg gggcgaggtg gcgctggaaa atactcaccc atttacccgc
    13681 gagttatggg ggcgtaactg gacttatgcc cataacggac aactgacggg ctacaaatca
    13741 ctggaaaccg gcaacttccg cccggtcggt gaaaccgaca gcgaaaaagc cttttgctgg
    13801 ctgctgcata aattaacgca gcgttacccg cgcacgccgg gcaacatggc ggcagtgttt
    13861 aaatatatcg cctcactggc ggatgaactg cgggcgaagg gcgttttcaa catgctgctt
    13921 tcggacgggc gctatgtaat ggcgtattgc tcgactaatt tacactggat cacccgccgc
    13981 gcgccgtttg gcgtggcaac gttgctggat caggatgtgg aaatcgactt cagctcgcag
    14041 accacaccga atgatgtggt cacggtgatt gcgacacagc cgctgacggg caatgaaacc
    14101 tggcaaaaaa ttatgccagg cgaatggcgc ttattttgcc tcggggagcg tgtagtttga
    14161 tgccagttgt ggctgcacca cttcgtggct taacggcttg ctgaccacgt aacggccatt
    14221 ggccacagaa acggtcggtg gcttacgggt ttgctcaaag tagtcgtagc ccggcttcag
    14281 ctgctcccag aagtccttaa agttggaata tttatggcgc ttcatattgg cgtcggtcat
    14341 gcggaatggg taaatactca cctgcacgct cggctgacca aacaccagcg caccagtaac
    14401 gaactggaag atctcatcaa taccctgatt ggtcattgcg tagcagccga tggatacaca
    14461 atcgccgtgg atcatcaggt atttcccttc ataaccatgc gcacggtcat aagcattggg
    14521 gaatccaata ttaatcgctt tgtagtaacg gctgtctggt tttaactgat tacgctggac
    14581 gctataaaac ccttccgggc ttttgaaatc gccctgacgc tgttttggcc ctaagccgcc
    14641 ggaatattta cagattttat aactgtcgag cagttgatat tgctcgccca ttttgacgta
    14701 aagatcgagc gtacgttctt ccttgaagat ctggatgtag acaggggatc ccatcaactg
    14761 ctgcttatac tctttgctca ctggcgtggt cgagctactg ctgcccagca aaccggcaaa
    14821 cgaaacgcac gggatcaaca acatcgcaag aattaatgcg attttacgca tactgcttat
    14881 tccttgataa aacggttaca cacgccagga cggcaaaatg gatcccaaat cggaatagtc
    14941 tggatttgga aggctcacat tatcaccaaa agagttttac gcaagcctgt cggcgctggg
    15001 tttacaaatt tagcacaggg gcagttttca atagtccgaa aagcgcatgt atcatttatt
    15061 tcctaatatt atgaaatctc aatagtaata tgcaaataag caattaataa tcccgtttac
    15121 tcctccggtc ttttaaaatt ctcaccttca ttcagccggt ttatgtcttt catgtcttcg
    15181 ttcttgctcg gcagattttt gcatccaggc gtgttttcgt tatgcgtttt gctcccttta
    15241 ctcgccagcg ccaccacaac cgggagcaga taaaacagca tccagaatgg tttcctgcgc
    15301 cgctcaaggc gagtgacaga cgctggcagg cgctggcgga aaacaaccac tttttaagca
    15361 gcgatcacct gcataacatc accgaagtgg cgattcaccg cctggagcag cagcttggca
    15421 agtcttacgt ctggggcggc acgcggcccg atcaaggctt cgattgtagt gggttagttt
    15481 tttatgccta caacaagatc cttgaggcga aactcccgcg cacggccaat gagatgtacc
    15541 actaccgtcg ggcaacgatt gtggcgaata acgacctgcg ccggggagat ttgctatttt
    15601 tccatatcca cagccgcgag attgccgacc atatgggggt gtatctgggc gacggtcagt
    15661 ttattgagtc actgcgcacc ggggaaacca ttcgggtaag ccggttagcc gaacctttct
    15721 ggcaggacca ttttttaggc gcgcgcagga ttttgacgga agagatgatt ttgtaggccg
    15781 gataaggcgt ttacgccgca tccggcggtt gtgcacggat gcctgatgcg acgctgacgc
    15841 gtcttatcat gcctacaaat ctgtgcgttt ttgcgagccg ctttcccgat ataacacagc
    15901 gtcattgtcg gcctttcctt ttcctcttac cgttgagaaa aaggagtcgc tatgtcggaa
    15961 tatcgtcgtt attacatcaa ggggggaaca tggtttttca cggtaaattt acgcaatcgt
    16021 cgaagccatc ttctgaccac ccagttccag acgctccgta acgccattat taacgttaag
    16081 cgagacagac cttttgaaat taacgcctgg gtcgttttgc cggagcatat gcattgcata
    16141 tggacattac ccgaaagcga tgatgatttt tcctctcgct ggcgggagat taaaaagcag
    16201 ttcacccatg cttgcggatt gaaaaatatc tggcaaccgc gtttttggga gcatgccatt
    16261 cgcaatacaa aagattatcg ccatcatgtt gattatattt atataaatcc agagaaacat
    16321 ggctgggtaa agcaggtgag tgattggcca ttctcaacgt tccatcgcga tgtcgcgcga
    16381 gggttatatc ccatcgattg ggcgggagac ataacggatt taagtgccgg ggagcgtatc
    16441 attttataac tgtgcgctgg tgccggatgc gcttacgctt atccggccta caaatcgtgc
    16501 tttaacggta ggccggataa ggcgttcacg ccgcatccgg cagttatgcg cagatgcctg
    16561 atgcgacgct ggcgcgtctt atcatgccta aaaacttgtg cctgaacggt aggccggata
    16621 aggcgtttac gccgcatccg gcaattgtgc accgatgccg gattcttaca tcaacgcccc
    16681 cataatcttc aactccagct catccggcac ttcgttatac gacagcacat gcagccccgg
    16741 cgcaaacaac cttgcataac gcggcagcaa agggcgtagc tgcggcggca ccagcagcac
    16801 cggatctttc cccgccgctt tcatctgctc tttcacctgt ggcatcgtgc tctggaactg
    16861 gttgagcata ttcgggtcaa ccggcacgct gtcgagcatc actttgccgg cctgttgcgc
    16921 ctgattcact acgttggtca gcagattttc cagctcatta ttcagcgtat acaccgtcag
    16981 ctcctgcttg cgaacgaacg gatgagtaat gctgcgccgc agcgccaggc gcacatcggc
    17041 cgccagcaga atatgatctt tagtcaccgt gctactggcg accagcacgg tggcgatggt
    17101 gacgatatcg cgcagggaaa cgccttcggt cagcagcgcc cggtacactt tcagcaattg
    17161 gctgtaattg agcgccgcgc ttaaatcctc cgccaggcgc ggtgccatcg acgacaaacg
    17221 gttatgcaac tgcgtgatgt catcatagtt aaacaaatca ggaatatagc tgcgcacaat
    17281 cttgttcaca tgggtggcaa tcacactggc gctgtcgatc acctgatacc ccatattcag
    17341 agccttcgct ttctgcgcag gctggatcca ggtgaccggt atcccgtacg ccgggtcgtt
    17401 ccccagcacg ccgtcaatct cgccgtaggt ttcgctggag ggcagcgcca tcagtttatc
    17461 cgccggaata tccgcttcat cagcctcaat gccgttgatg aaaatagcgt actggctggg
    17521 cttgaggcgg aagttttccc gaatgcggat ctccggcagc agcacgccgt taccgtcgga
    17581 aatcacctgc cgaacgccgc gaatccgctg ggtgagcggg ttgccctgcg ctttgtccac
    17641 cagcgccacc agcttgtagc cgaggcttaa gctgatgggc tcgatcagcg gaatagtttc
    17701 ccaactgacc tgttgttcgc tggtttcagt gatggtgcgg gtcagcgttt cgaggctttt
    17761 ctcttccgcc tccgccgcct gcgggcgttt gctcatccgc cagccggtaa agccaagcag
    17821 ggcgctgaac agcaaaaacg gcagatgcgg catccccggc accaccgcca gcacgaacat
    17881 gatcccggtg gcggtgtaga gcaccgacgg gcttgccagc aactggtggc gcacgtcatg
    17941 ggcgatatcg ccgttgtcgc tgacgcgggt gacgataatc gccgccgcgg tggagagcag
    18001 cagggaaggg atctgcgcca ccaggccgtc gccgatggtc atcagcacgt attgctggaa
    18061 ggcggcgtcg gcgctcaggt tgtatttgaa gatgccgata cagacgccgc cgctcaggtt
    18121 gatcgccaga atcatcatcc cggcgatggc gtctccgcgt acaaacttcg atgccccgtc
    18181 catcgcgccg tagaaatcgg cctcgctggc aacatcttta cgccgggttt gcgcctgcgc
    18241 ctggttgatc aatccggcgt taagatcggc gtcaatcgcc atctgtttgc cgggcatcgc
    18301 gtccagggta aagcgggcag aaacctcgga aatacgctcg gcctctttgg tgacgacgat
    18361 aaagttgatt gtcaacagcg tcagtaaatc cgagcgtgaa agcattatcg ccgcgctgca
    18421 cgggcagtcg atttttaacg gtggtgggct gtcgccgtta aataaaatca gcccgtctca
    18481 tccgccaaaa cctgccaccg tggcggtgcc ggaagagacg gagaaaaaag cgcgtgatgt
    18541 gaatgagaaa acggcgctgc tgaagaagaa atccgccacc gagcttggcg agttggcaac
    18601 cagtatcaac accattgccc gtgatgcgca tatggaagcg aatctggaga tggagattgt
    18661 tccccaggga ttacgcgtgc tgattaaaga cgaccagaat cgcaatatgt tcgaacgcgg
    18721 cagcgcgcag attatgccgt tctttaaaac tttgctggtg gagctcgcgc cagtgttcga
    18781 ctcgctctat aacaaaatta ttattaccgg gcataccgat gcgatggcct acaaaaacaa
    18841 tatctacaac aactggaacc tttcgggcga tcgcgcgctt tcagctcgtc gggtgctgga
    18901 agaggccgga atgccggaag ataaagtgat gcaggtaagc gcaatggcgg accagatgct
    18961 gctggacgcg aagaatccgc aaagcgcggg caatcggcgt attgagatta tggtgctgac
    19021 caaaagtgcg tccgatacgc tgtatcaata ctttggtcag catggggata aagtggtgca
    19081 gccgctggtg caaaagctgg ataagcagca ggtgctttcg cagcgaatgc gctaaatgct
    19141 gaatctttac gcatttctca aaacctgaaa tcactgtata ctttaccagt gttgagaggt
    19201 gagcaatgcg taaaatcatt catgtggata tggactgctt tttcgcagcg gtggagatgc
    19261 gcgacaaccc cgctctgcgc gatatcccta ttgctattgg cggcagccgc gaacgtcggg
    19321 gggtgatcag caccgccaat tatcccgcgc gtaaatttgg cgtacgtagc gctatgccta
    19381 ctgggatggc gctcaaatta tgcccacatc tcaccttgct tccggggcgc tttgacgcct
    19441 acaaagaagc ctcaaatcat atccgtgaaa tcttctcgcg ctacacctcg cgcattgaac
    19501 cgttgtcact ggatgaggct tatctcgatg tcaccgatag cgtccattgc cacggttctg
    19561 cgaccctcat cgcccaggaa atccgccaga cgatcttcaa cgagctgcaa ctgacggcat
    19621 ctgcgggcgt ggcaccagta aagtttctcg ccaaaatcgc ctccgacatg aataaaccca
    19681 acggtcagtt tgtgattacg ccggcagaag ttccggcatt tttacaaacc ttaccactgg
    19741 aaaaaatccc cggcgtcggc aaagtctcgg cggcaaaact ggaagcgatg gggctgcgga
    19801 cctgcggtga tgtacaaaag tgtgatctgg tgattctgct taaacgcttt ggcaaatttg
    19861 gccgcatttt gtgggagcgt agtcagggga ttgacgagcg cgacgttaac agcgaacggt
    19921 tacgaaaatc cgtcggcgtg gaacgcacga tggcggaaga tattcatcac tggtctgaat
    19981 gtgaagcgat tatcgagcgg ctgtatccgg aacttgaacg ccgtctggca aaggtaaaac
    20041 ctgatttact gattgctcgc cagggggtga aattaaagtt cgacgatttt cagcagacca
    20101 cccaggagca cgtctggccg cggctgaata aagctgactt aatcgccacc gcgcgtaaaa
    20161 cctgggatga acgccgcggc gggcgcggtg tgcgtctggt ggggctgcat gtgacgttgc
    20221 ttgacccgca aatggaaaga caactggtgc tgggattatg atgtatacta ttatgtatat
    20281 tctggtgtgc attattatga gggtatcact gtatgcatcg aattctcgct gaaaaatcgg
    20341 tcaatatcac tgagttacgt aaaaacccag ctaaatactt tattgatcaa ccagttgcgg
    20401 ttctttctaa taatcgcccc gcaggatatc tcttaagtgc cagcgcattc gaagcgttaa
    20461 tggacatgct tgctgaacaa gaggagaaaa agcccataaa ggcgcgcttt cgtccaagtg
    20521 ctgcaagatt agaggaaatt acacgccgcg ctaaacaata tcttaatgat atgacggatg
    20581 atgatttcaa tgactttaag gaataaggat gcgggtattc aaaacaaaac ttattcgcct
    20641 gcaacttaca gcagaggaac ttgatgcgtt aacggcggat tttatttcct ataagcgtga
    20701 cggtgttttg ccagatatat ttggtcgcga tgcactctac gacgactcgt ttacctggcc
    20761 attaatcaaa tttgagcgag ttgctcatat tcatctggca aatgtgaata atccatttcc
    20821 gccacagttg cgccaattca gcagaacgaa tgacgaagcg catttggtat attgtcaggg
    20881 tgcgtttgat gagcaagcat ggttgctcat tgccattctg aagcctgaac ctcataaact
    20941 ggctcgagat aacaaccaaa tgcataaaat tggaaaaatg gcagaagcgt ttcgcatgcg
    21001 tttttgaatt tatattatga ataacataca aataagagat tatcagcctg gcgattttca
    21061 gcaactatgc gctattttca ttagagcggt tacgatgacc gtcagtcagc attattcact
    21121 acaacaaatt gccgcctggg cgcagattga cgaatctcgc tggaaggaga aactcgcgaa
    21181 atcacaagtg cgggttgcag ttattaatgc aaaactggtc ggctttatta cctgcgttga
    21241 acactatatc gatatgttat ttgttgaccc tgaatacacc cgccgtgggg ttgccagcgc
    21301 tttgttaaaa ccttttatta agtctgaatc cgaacttacg gtggacgcaa gcataaccgc
    21361 aaaacccttt tttgaacgtt atggttttca gacagttaag cagcagcgcg ttgaatgccg
    21421 gggagcgtgg tttattaatt tttatatgcg ttataaaccg caacattaaa tccagcttgc
    21481 aatgaaaata acgcccgcct agtatgtgcg cgttttctta ttccacaaac tgcaaggagg
    21541 taaatcatga caaatccttt atcaatgact ctttgcagac ctttccagga ttaattcttt
    21601 ttttcttgcc ctggattcgt ctgccatttc ctgatttttt aattgttact ctcaataatt
    21661 caagtcgcag cacatgcaac ttgaagtatg acgagtctat ttacggaatg gtatatgggc
    21721 aattatattc gtcccttatc cgatgcggta tttaccatcg catctgatga cctgtggatc
    21781 gagagtttag cgatccaaca attacacacc acggcaaatt tacccaacat gcagcgcgta
    21841 gtggggatgc cagatttaca ccccggacgc ggctacccga ttggcgcagc gttcttctcc
    21901 gcaggccgtt tttacccagc actggtcggc aatgatatcg gctgtggcat ggcgctatgg
    21961 caaacagata ttctcgctcg caaatacaac gccgataagt ttgaaaagcg attatcagct
    22021 ttggatgacg ttgctgaaga aagctggctg gaggaaaacc tgccgtcagc atttgcacag
    22081 catccgtggc gcagctcgct tggctctatc ggtggtggca atcatttcgc tgaactgcaa
    22141 caagttgatc aaatcatcaa cgctgaactg tttgcactgg caggtctgga tgcgcagcat
    22201 ctgcaactgc tggttcatag cggctcgagg gggctagggc agtctatttt acagcggcat
    22261 attgcctcgt tttcgcatca tggtttgcct gaaggcagtg acgacgcgct ggcgtttgcg
    22321 cgttttaatc gccacctgat cgctttgcgc ataatgcaac aggttaaggc caccggaagt
    22381 ccggttctgg atgtggcgca taactttgtt agcgcgtgtc gaatcggtga tcaacaaggg
    22441 gtcttacatc gtaaaggtgc cacgcctgat gactgtggtc tggtggttat tcccggttca
    22501 cgcggtgatt actccaggct ggttcagccc gtcaggagtg aggaaacatt gcattcgctg
    22561 gcgcatgggg ctgggcgtaa atgggggcgc accgagtgta aagggcgtct ggcagcgaaa
    22621 tacacagcga cgcaactctc gcgtactgaa cttggcagtc gggtaatttg tcgcgataaa
    22681 caactcatct tcgaagaagc gccacaagcc tataaatcgg ctgaaagtgt ggtgcaatgt
    22741 cagggcaggc cggattaatt attcctgtcg cgcggctgcg tccggtgcta acgctcaaaa
    22801 acagtggagg gaaaaaagga tgatcttgct ataactctcc tcagcgcagg ggccggaaga
    22861 atgttgtctc gcagtgaaaa aggcgctgga caggctaatt aaagaagctg cccgacaaga
    22921 cgtcgcggta acggtgctgg aaacagaaac aggccgctac tctgacacgc tgcgttcggc
    22981 gctgatttct ctggatggcg acaacgcatg ggcgttaagc gaaagctggt gtggcactat
    23041 tcagtggatt tgcccgagcc cgtatcgccc acatcacggg cgtaaaaact ggtttctggg
    23101 cattgggcgt tttaccgctg atgagcagga gcaatccgat gcgatccggt atgagacgct
    23161 gcgttcgtcg gggccgggcg gtcaacatgt caataaaacc gactcggcgg tacgcgccac
    23221 gcatttggca tccggtatta gcgtgaaggt tcagtcagag cgcagccagc atgctaacaa
    23281 acggctggcg cgattgctga ttgcctggaa gctggatcag cagcaatagg aaaatagcgc
    23341 ggtgctgaaa tcacagcggc gaatgttcca tcaccagatt gaacgtggca acccgcgacg
    23401 aacgtttaca gggatggctt ttatcgaagg ataatgaagg atgaaactgc cggaaggcga
    23461 ttaaacgcca tccggcagca aatcaaataa ttacttcgcc ggaatttctt tcagcagttc
    23521 agtcagcagt gtccagtaat gacctacgct ttcgatgtga acttgctcat ccggagagtg
    23581 tggaccggtg atagttggcc cgatagaaac catgtccatt tccggatacg gttttttgaa
    23641 cagaccacat tccaggcccg cgtggataat ctggatgttc ggcgtcttgt tgaacaggcg
    23701 ctgataggtt tcacgtacca gatgcatcac cggagaatta gcgtccggct gccagccagg
    23761 atatgcgcct ttcgcttcgg ttttcgcgcc agccagttta cccagcgaat ccagcatgct
    23821 caccacgtag tctttaccgc tgtcgattag tgaacggatc aggcagtgaa tttctacgtt
    23881 attgtcagtc atggtcacca caccgacgtt cagggaggtt tcgaccacac cttttgccac
    23941 gtcggaattg cggatcacac cgttcggggt ggcgttcagc agacgaataa aggtatcgcg
    24001 agatttcgca atcagggcag ctttatcgtt cgctacagag tccagcaaca aggccagatt
    24061 tttctctttc tctgccagct cgtttttcag gatctcctga taggtattca ccagagattt
    24121 cagagcgtcg actttatcag ctgcgacagc aatggtcgca aaggcttcac gcgggatggc
    24181 gttacgcagt gtgccgccgt tgaaatcgat aaggcgcagg tccagttctt ctgcatgacc
    24241 cgccaggaag cgcaccagca gtttgttggc attacccagg ccaacgtgga tttcaccgcc
    24301 ggagtgaccg cctttcagac cttttaaggt taacttgaag gtttcaaaac cagctggaac
    24361 cgcttcacga tctaaatgca ggttggaggt gaagtcgata ccccccgcac aacccatgta
    24421 gatttcacct tcttcttcgg agtcggtgtt aatcagaata tcagcctgca accagttgct
    24481 ctgtaagccg aacgcaccgt ccataccggc ttcttcggtc atggtcagca gcacttccag
    24541 cgggccgtga accacgtttt cgtcagccag aaccgccagt gcagaggcca taccaatgcc
    24601 gttatccgca cccagcgtgg tgccgcgcgc tttaacccat tcgccatcaa tataaggctg
    24661 gataggatct ttcgtgaagt catgcacggt gtcgttattt ttctgcggca ccatatcgag
    24721 gtgggcctgt aagacgaccg gtttacgatt ttccatacct gcggtagcag gttgacgaat
    24781 caggatatta cctacctgat cgcgttcgac atggaaacct ttctcttttg cccaaccaac
    24841 aatgtattca gcgagttgct cttcatgata ggacgggtga ggaatagaac agattttggc
    24901 aaaaatatcc cacagcggct gtggagataa ttgagacagt tcagacacgt taagtctcct
    24961 tgtcgatcac ccgcaaaaca gtattgcagg tcacagggtt agcagaaaat gttgtcaaca
    25021 caagacaggc ttgcgagata tgtattgaga ataccacttt atcccgcgtc agggagaggc
    25081 agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat
    25141 aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg
    25201 acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt
    25261 aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt
    25321 ggcggtctgg taccgggggc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc
    25381 gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca
    25441 gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgct
    25501 gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct
    25561 ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg gatcgaacag
    25621 ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc
    25681 ctggcattgc cgggcgttgt tctttttaac ttcaggcagg ttacaatagt ttccagtaag
    25741 tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg
    25801 ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc
    25861 tgtgcagtcg gctcttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct
    25921 gatgtggatc tggcgcggta ttgacccacg tgaaatcctc gacgtccagg cacgtattgt
    25981 gatgagcgat gctgaacgta ccgacgatga tttatacgat accgtgattg gctaccgtgg
    26041 tggcaactgg atttatgagt gggccaccca ggcgatggtg tggcaacaaa aagcctgtac
    26101 ggaagaagat ccgcaactca gtggtcgaca ctggctgcat gcggctacgt tgtacaacat
    26161 tgccgcctat cctcatctga aaggagatga cctggccgag caagcgcagg ctttatcaaa
    26221 ccgcgcctat gaagaggccg cccagcgtct gccgggcacg atgcggcaga tggagtttac
    26281 cgtacccggc ggtgcgccca tcaccggctt tttgcatatg ccgaaaggcg atggcccgtt
    26341 cccgacagta ttaatgtgtg gtggtctgga tgcgatgcag acggactatt acagcctgta
    26401 tgaacgttat tttgcgccgc gcggcattgc gatgctgact attgatatgc cgtcggtggg
    26461 cttttcttca aaatggaagc tcacccagga ctccagcctg ttgcatcagc acgtcttaaa
    26521 ggcgctgcct aacgtaccgt gggtggatca cactcgcgtc gcggcctttg gtttccgttt
    26581 cggcgctaac gttgccgtgc gtctggcata ccttgaatcg ccgcgtctga aagcggttgc
    26641 ctgtcttggt ccggtagttc ataccctgtt gagtgatttt aagtgccagc aacaggtgcc
    26701 ggaaatgtat cttgacgttc tggcgagtcg tttggggatg catgatgctt ccgacgaagc
    26761 gttacgcgtg gagctgaatc gctattcatt aaaagtgcaa ggattgctgg ggcgtcgctg
    26821 cccaacgcca atgttatcag gctactggaa gaacgatccg ttcagcccgg aagaggactc
    26881 acgcttaatc acctcatcat ctgctgacgg taaattatta gagatcccat ttaacccggt
    26941 gtatcggaat tttgacaaag gtcttcagga aatcaccggc tggatcgaaa aacgcttgtg
    27001 ttaaaaattt gctaaatttt gccaatttgg taaaacagtt gcatcacaac aggagatagc
    27061 aatgacgtta ccgagtggac acccgaagag cagattgatc aaaaaattta ccgcactagg
    27121 cccgtatatt cgtgaaggta agtgcgaaga taatcgattc tttttcgatt gtctggctgt
    27181 atgcgtcaac gtgaaaccgg caccggaagt gcgtgaattc tggggctggt ggatggagct
    27241 ggaagcgcag gaatcccgtt ttacctacag ctaccagttt ggtctgttcg ataaagcagg
    27301 cgactggaag agtgttccgg taaaagatac tgaagtggtt gaacgactgg agcacaccct
    27361 gcgtgagttt cacgagaagc tgcgtgaact gctgacgacg ctgaatctga agctggaacc
    27421 ggcgaatgat tttcgtgatg aaccggtgaa gttaacggcg tgagtgaaat gtgccggatg
    27481 catcacatcc ggcaatattc attaaaactg atacgtcatc ccaaccgcga cagtatcatc
    27541 attattaata ttcagcttat tatcgctatc cagttggttg attttataat caacaaacgc
    27601 tgacatattt ttgttgaaat aatacgtagc accgacgtcg atataattga ccagatcttc
    27661 atcaccgatg ccctcaatat ctttcccttt cgataagaca taacccagcg atggacgcag
    27721 accaaagtca aactggtatt gagcgaccgc ttcaaagttc tgtgtcttat tggcaaagcc
    27781 gccagttatt ggcgtcattt tgcgtgtttc agaatagaaa gttgccagat aaatattatt
    27841 ggcatcgtat ttcagacctg ttgcccatgc ttctgcacgc ttgcctgtgc cacggctttg
    27901 caggttctgc tcgttggtgc gatctgagtt ggtataggcc ccactaatgg cgaaatcgct
    27961 gccgccaaag tcatatgtca atgacgtgcc gaagccatcg ccgttttgct ttttaacgtc
    28021 gcggttttcg tttttccctt gatattgcag ggttaagttc aggccatcga taacgccgaa
    28081 gaagtcggtg ttccgatacg tcgccagacc gctggcgcgt ttggtcataa agttgtcggt
    28141 ctgcgccgag gaatcgccac caaattccgg gaacatatcg gtccaggctt ccacgtcata
    28201 caacgcgccc aggttacgac cataatcgaa agaacccaaa tctttatatt tcaacccggc
    28261 aaaagcgaga cgcgtttttt gctgtgcagt atcactctcc gctttatttc cggcaaactc
    28321 cgcttcccac cgaccataac cagtcagttg atcgttaatt tgagtttcac ctttaaaacc
    28381 aaaacggata taactctggt cgccatcttt actggcgtta tcactcatat aatgcatggc
    28441 tttaactttg ccatagacat ccagtttatt accgtcttta ttatatattt ctgcagcctg
    28501 tacagatgca gatgccacaa tgcccatcac cactaatgcc agagtgctct ttttcatttt
    28561 cattcctgat tttaattaac gcgcgaatat tcagcgggag agccccgttg aaaacaggaa
    28621 agtttttaac ctgagattgt taaagatata ttacagatta atgatatt
//
LOCUS       DAJTSA010000048        28420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000048 DAJTSA010000000
VERSION     DAJTSA010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.048"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            564..1334
                     /locus_tag="OO369_002223"
     CDS             564..1334
                     /locus_tag="OO369_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR5982485.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="OO369_002224"
     CDS             1325..1798
                     /locus_tag="OO369_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR5982486.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="OO369_002225"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="OO369_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR5982487.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="OO369_002226"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="OO369_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR5982488.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="OO369_002227"
     CDS             3233..3727
                     /locus_tag="OO369_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR5982489.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3967..6258
                     /gene="opgB"
                     /locus_tag="OO369_002228"
     CDS             3967..6258
                     /gene="opgB"
                     /locus_tag="OO369_002228"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR5982490.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6396..7418)
                     /gene="lgoD"
                     /locus_tag="OO369_002229"
                     /pseudo
     CDS             complement(6396..7418)
                     /gene="lgoD"
                     /locus_tag="OO369_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7557..8471
                     /locus_tag="OO369_002230"
     CDS             7557..8471
                     /locus_tag="OO369_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5982491.1"
                     /translation="MSRSQNLRHNVINQVIDDIARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8686..10047
                     /locus_tag="OO369_002231"
     CDS             8686..10047
                     /locus_tag="OO369_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5982492.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10096..11760)
                     /gene="tsr"
                     /locus_tag="OO369_002232"
     CDS             complement(10096..11760)
                     /gene="tsr"
                     /locus_tag="OO369_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR5982493.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11879..12325)
                     /gene="hpaR"
                     /locus_tag="OO369_002233"
     CDS             complement(11879..12325)
                     /gene="hpaR"
                     /locus_tag="OO369_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR5982494.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12597..13886
                     /locus_tag="OO369_002234"
     CDS             12597..13886
                     /locus_tag="OO369_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR5982495.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13883..15349
                     /gene="hpaE"
                     /locus_tag="OO369_002235"
     CDS             13883..15349
                     /gene="hpaE"
                     /locus_tag="OO369_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR5982496.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15351..16202
                     /gene="hpaD"
                     /locus_tag="OO369_002236"
     CDS             15351..16202
                     /gene="hpaD"
                     /locus_tag="OO369_002236"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR5982497.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16212..16592
                     /locus_tag="OO369_002237"
     CDS             16212..16592
                     /locus_tag="OO369_002237"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR5982498.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16660..17463
                     /gene="hpaH"
                     /locus_tag="OO369_002238"
     CDS             16660..17463
                     /gene="hpaH"
                     /locus_tag="OO369_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR5982499.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17474..18277
                     /gene="hpaI"
                     /locus_tag="OO369_002239"
     CDS             17474..18277
                     /gene="hpaI"
                     /locus_tag="OO369_002239"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR5982500.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18299..19654
                     /gene="hpaX"
                     /locus_tag="OO369_002240"
     CDS             18299..19654
                     /gene="hpaX"
                     /locus_tag="OO369_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR5982501.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19664..20554
                     /gene="hpaA"
                     /locus_tag="OO369_002241"
     CDS             19664..20554
                     /gene="hpaA"
                     /locus_tag="OO369_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR5982502.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            20581..21518
                     /locus_tag="OO369_002242"
                     /pseudo
     CDS             20581..21518
                     /locus_tag="OO369_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21745..23303
                     /gene="hpaB"
                     /locus_tag="OO369_002243"
                     /pseudo
     CDS             21745..23303
                     /gene="hpaB"
                     /locus_tag="OO369_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23321..23833
                     /locus_tag="OO369_002244"
     CDS             23321..23833
                     /locus_tag="OO369_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR5982503.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24226..26376
                     /gene="btsT"
                     /locus_tag="OO369_002245"
     CDS             24226..26376
                     /gene="btsT"
                     /locus_tag="OO369_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR5982504.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26426..26629
                     /locus_tag="OO369_002246"
     CDS             26426..26629
                     /locus_tag="OO369_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5982505.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26640..27596
                     /gene="yjiA"
                     /locus_tag="OO369_002247"
     CDS             26640..27596
                     /gene="yjiA"
                     /locus_tag="OO369_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR5982506.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27665..>28420)
                     /locus_tag="OO369_002248"
     CDS             complement(27665..>28420)
                     /locus_tag="OO369_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HCR5982507.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFY
                     LTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6955 a         7426 c         7126 g         6913 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tgggggctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtgcgtt
     1741 atccatcggt ctttccattc ctggattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccgggaagcg
     2101 atgtcgctct ggacgatcaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggcg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcgct cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaagcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact caatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaatgtt acccggctga cgcggctctc tcctgccctt ttccctgctc
     3781 ctggacggtt ttccccctgt tacacgggaa gcgaccgcga tcaaaaaagt ctgtttataa
     3841 actaattcct aacaacatcg catttttttg acactagaat tgcagcaata tggcgacgcc
     3901 attacattgt gcttctcaaa aagcttaaac gttggctaaa cggcccggtt tagtgaggat
     3961 cgcccattgt cagaactact ctctttcgcc ctttttctcg cctctgtgct gatttacgca
     4021 tggaaagcgg gacgtaacac ctggtggttt gcagccacgt taacggtgct ggggctattt
     4081 gtcgttttaa atatcaccct gtttgccagc gactatttta ctggcgatgg tattaacgac
     4141 gcggttctct ataccttaac caacagcctg accggtgctg gcgtcagcaa atacatcctg
     4201 ccgggtatcg gcattgtgct ggggctgacg gcggtgttcg gtgcgctggg ctggatcctg
     4261 cgccgtcgtc gtcatcatcc gcaccatttt ggttacagcc tgctggcgct cttactggcg
     4321 ctgggttcgg tggacgccag cccggcattt cgtcagataa cggaactggt gaaatcccag
     4381 tcacgcgatg gcgacccgga ctttgcggct tattataaag agccgtcgaa aactatccct
     4441 gacccgaaac tcaacctggt ttatatctac ggcgaaagtc tcgagcggac ctattttgat
     4501 aacgaagctt tcccggatct cacgcctgaa ctgggcgcgt tgaaaaatga aggcctggat
     4561 ttcagccaca cgcagcagct gccaggaacg gattacacga ttgcgggcat ggtggcttct
     4621 cagtgcggca taccgctgtt cgcccccttt gaaggcaacg cctccgcctc tgtctccagc
     4681 ttcttcccgc agaacatctg tctgggcgat atcctgaaaa actcgggtta tcagaactat
     4741 ttcgtgcagg gcgcaaatct gcgttttgcc ggtaaagatg tgttcctgaa atcgcacggc
     4801 ttcgaccact tatacggctc agaagagctg aaaagcgtgg tagccgaccc gcactatcgc
     4861 aacgactggg gattctacga cgataccgtt ctcgatgaag cgtggaaaaa gtttgaagag
     4921 ctttcccgct caggtcagcg attctcactg tttaccctga cagtcgatac ccatcacccg
     4981 gatggtttta tctcgcgcac ctgtaaccgc aaaaaatatg attttgacgg taagccgaat
     5041 cagtcattca gcgcggtaag ttgcagccag gagaatatcg cgacgtttat caacaaaatc
     5101 aaagcgtcac cgtggtttaa agatactgtc atcgtcgtct cttctgacca tttagcgatg
     5161 aacaacacgg cgtggaaata cctcaataag caggatcgca ataacctgtt ttttgtcatt
     5221 cgtggcgaca agccgcagca aaagacgctg gcagtgaagc gtaacacgat ggataacggc
     5281 gcgacggtgc tggacattct cggtggcgat aactatctcg gacttggtcg tagcagttta
     5341 tccgggcagt cgatgtcgga aatcttcctc aatatcaaag agaaaacatt ggcgtggaag
     5401 ccagatatca tccgcctgtg gaaattccct aaagagatga aagagttcac catcgaccag
     5461 cagaaaaaca tgattgcctt ctcgggtagc catttccgtt tgccgctgct gttgcgggtt
     5521 tcagacaaac gcgtggaacc gctgccggaa agcgaatact cagcaccgct gcgtttccag
     5581 ctggccgatt tcgctccacg cgacaatttc gtctgggttg acagttgcta caagatggca
     5641 caactctggg ctccggaact ggcactttcc accgactggt gtgtctcgca ggggcagctt
     5701 ggcggtcagc aaattgttca gcatgttgac aaaacaacat ggaagagcaa gacggcattt
     5761 aaagatacgg tcatcgacat ggcgcgttac aaaggcaatg tcgatacgct gaagattgtt
     5821 gataacgata ttcgctacaa agccgacagt ttcatcttta acgtcgccgg tgcgccagaa
     5881 gaggtgaaac agtttagcgg gatttcccgt ccggagtcgt ggggccgctg gtccaacgcg
     5941 cagctgggcg atgaagtaaa aatcgagtac aagcatccgc tgccgaagaa atttgacctg
     6001 gtgattaccg ccaaagcata cggcaataac gccagccgtc ctattccggt acgcgtaggc
     6061 aatgaagaac aaactcttgt gctgggcaat gaagtgacca ccaccacact gcatttcgat
     6121 aacccaaccg atgccgacac actggtaatt gtgccgccgg aacctgtctc aaccaacgaa
     6181 gggaatatcc tcggacactc gccgcgtaag ctcgggatcg gcatggtgga aattaaagtg
     6241 gtagaacgtg aagggtaatt cctgactgat gtgtaggtcg catagggtgc gacctacacg
     6301 ccttctccgg cttacaacac attattaaga aacaaaaaaa acagcgccca acacttcccc
     6361 ggacgctgca tggcaacttt aataatctcg ccttatcaga aagtaattac acccttaatc
     6421 aactcacgat tgttaatcac atcgcgctcg taggtttctg ccagcgtggc gaacggatag
     6481 cgatgggtta acatcatgtc ggcagtgatt ttcccttccg ccatcagtcg accgacttta
     6541 gcgaaatctt ccggcgtggc gttgcggctg cccatcatcg tcgtttcttt tttatggaat
     6601 tccggatcgg agaactgcaa ctcaccttta aacagaccga caaataccac cgtgccgccg
     6661 tgacgaatca gattcacggt attattcatc gcatgttgat tacctgtcgc gtcgatcact
     6721 ttctgcgcca gcgaaccacc aaactgcgcc cgcagctgcg cgtcaaaatc ttcggctgac
     6781 gggtccagca cgggcaactc cagacgcgtt gccacatgtt cacggcgcgc cggactggta
     6841 tccgccacca ccacctgtgc gccatcggct ttagcgattg ccgccgcgcc cagaccgatt
     6901 ggccccgccc cgaccaccag cacctgctcg ccgggagcaa tggctgcgcg acgcaccgca
     6961 tgagcgctaa tagcgaaagg ttcaatcaat gcggccgcct gcgggtcaat accgtctgcg
     7021 ggcaaaatgt tcgccaccgg cacgctcaaa tactcactaa aaccgccatc ctgatgcacg
     7081 ccaatgactg aaattttttc acagcagttg gtacgcccgc ttttacacgc cgggcattgc
     7141 tgacaggcaa cataagggat cacagcaact tgctgaccat ttttaagatc agcaatattt
     7201 ttacccagcc caacaatctc cccacatatt tcatggccta aaacccgtgg ataactaaaa
     7261 aatggttgat ttccacccta ggcatgaata tcggtaccgc aaatcccgac agacttaatt
     7321 tttattaatg cttcattgtc acccggaata ggtatctcgc gttgtttcca gactaattct
     7381 ttcggctgct ggcaaattaa aacattcatc gtagacatga ttcggctatc cattttttgt
     7441 tgccttagat atcaatgaaa atgaaaaatg gcagcggaac ggaaaatctg ttttgtgaaa
     7501 acacacgcat aaaaatgttt taaatcgggt tttaatggac tcacaaggag gaaaatatga
     7561 gtcgttcaca aaatttacgc cacaatgtga ttaaccaggt cattgatgat atagcgcggg
     7621 gtcatatccc ttccccactt ccatcgcaaa gtgcgctggc ggaaatgtac aacatcagcc
     7681 gcactacagt gcgtcatatt ctcagccaat tacgcgaatg cggcgtctta acgcaggttg
     7741 gaaacgacta tgttattgcg cgtaaacctg accatgatga cggtttcgcc tgtactaccg
     7801 cttcaatgag tgaacaaaac aaagtgtttg agcaggcttt tttcacgatg ataaaccagc
     7861 gtcagttacg ccctagggaa actttttctg aactgcaact ggctcgggcc gcgggtgtca
     7921 gcccggtggt tgtaagagaa tatcttttaa aattcggccg ttataatctt attcaaagcg
     7981 aaaagcgcgg ccagtggagc atgaagcaat tcgatcaatc ctacgccgag cagctgtttg
     8041 agctacgcga aatgctcgaa acacattcac ttcagcactt tctcaacctg cctgatcatg
     8101 atccccgctg gctgcaggcg aaaaccatgc tagaacgtca tcggctactg cgtgacaaca
     8161 tcggcaatag ctttcgcatg ttctcgcaac ttgacagaga ttttcactcc ttactgcttt
     8221 cagctgccga caatatcttt tttgatcaat cgcttgagat catctcagtc atctttcatt
     8281 ttcactatca atgggacgaa agcgatctta aacagcgcaa tatcattgcc gtcgatgaac
     8341 atatgaccat tctcagcgct ctgatttgcc gcagtgacct tgatgccacc cttgcgctcc
     8401 gtaaccattt gaattcagct aaacaatcaa tgattcgatc cattaatgag aacacgcgtt
     8461 atgctcatta agtacggatt aaaaacagca aactattagc gggtgcacgg ttttatcaaa
     8521 agataaatac cgggaaattc ccgcttacct atgctcacaa tcagtcgact aaaaatgttt
     8581 ttgtgagcgg tagtaaagtc ctaaaacttt aacctgccgt taatgtcaac cttgcgtaat
     8641 gcacaattac gtaaacaatt aaaattataa atacaggagt ccggcgtgga aaaagaaaat
     8701 atcaccctcg atccgcgttc ttcatttact ccatcttcgt cggcagatat tcccgtgcca
     8761 ccagatggat tagttcaacg cagtacccga attaaacgca ttcaaaccac cgccatgttg
     8821 ttattatttt ttgcggcggt aatcaattat ctcgaccgca gttcgctgtc ggtagcaaat
     8881 ttaacgattc gtgaagaatt gggattaagt gccaccgaaa tcggcgcttt gctctccgtg
     8941 ttttcactcg cttacggcat tgcgcaactt ccttgcggcc cactattgga tcgtaaaggc
     9001 ccacgcctga tgctgggact ggggatgttc ttctggtcac tgttccaggc catgtctggc
     9061 atggtgcaca gctttacgca gttcgtgttg gtgcgtatcg gtatggggat tggtgaagcg
     9121 ccgatgaacc catgcggtgt aaaagtcatt aacgactggt tcaacatcaa agagcgcgga
     9181 cgcccgatgg gcttcttcaa cgcagcttct accattggcg ttgctgtaag cccaccgatt
     9241 ctggcggcaa tgatgctggt gatgggctgg cgcgggatgt ttattaccat tggtgtactg
     9301 gggatttttc tcgccatcgg ctggtatatg ctctatcgca accgcgagca cgtagaactg
     9361 actgccgttg aacaagctta tctcaatgca ggtagcgtca atgcccgccg agatccgctc
     9421 aattttgccg aatggcgcag cctgttccgt aaccgcacaa tgtggggaat gatgctcgga
     9481 ttcagtggca tcaactacac tgcgtggctg tatctggcct ggcttcctgg ttacctgcaa
     9541 acagcctata acctggattt aaaaagcaca gggttgatgg cggctatccc tttcctgttt
     9601 ggggctgccg ggatgctggt caacggttac gttactgact ggctggtcaa agggggaatg
     9661 gctccgatta aaagccgtaa gatctgcatt attaccggga tgttctgttc tgccgccttt
     9721 acgctgatag taccacaagc gacaacatcc atgatggcgg ttctgctgat tggcatggca
     9781 ctgttctgta ttcactttgc cggaacatcc tgctggggct tgatccacgt cgcagttgct
     9841 tctcgcatga ctgcgtcggt gggcagtatc cagaactttg ccagcttcat ctgcgcctct
     9901 ttcgcgccga tcattactgg ttttattgtt gatacaaccc actcattccg tctggcacta
     9961 atcatttgcg gttgcgtcac cgcagcggga gcactggcgt acatcttcct ggttcgccag
    10021 ccgatcaacg acccgcgtaa agattaaact ccctatgccc gataacattt tgcttatcgg
    10081 gcatttttat ggcgattaaa acgtttccca gttctccccg ctatctgcca ctgccatttt
    10141 acgcggcgta gctggcgtca cggtttttac cacagccgat gtttcacgct gctgttgctg
    10201 ctgttgctga atccggaaca ctgccacagc ttcggtcagg cgactggcct gctcttccag
    10261 cgcggcggcg gcagcggcag attcttccac cagcgcggcg ttctgttgag ttacccggtc
    10321 catctcagca accgctaagc caacctgatc gataccacgg ctctgctcat cagaagcaga
    10381 agctatttcg cccataatgt ccgtcacgcg ggtcacagca ctgacaatct ccgccattgt
    10441 ttccccggcg ctttcgacca gcgtagagcc aacatccact ttccccaccg agtcttcaat
    10501 caggctttta atttcacgaa ccgcctgggc gctacgctgg gccagattac gcacttctcc
    10561 cgcaaccacc gcaaaaccac gtccttgctc acccgcacgc gctgcttcaa ccgccgcgtt
    10621 caaagccaga atattggtct ggaaggtaat gccgtcaatt acgctgataa tatcggcgat
    10681 tttctgcgaa ctggtggaga tatcgcgcat agtctgcacc acgttatcta ccactttgcc
    10741 gccgcgttgc gccgtttcag aagcacttaa tgccagatgg ctggcctggc gcgcattctc
    10801 ggcgttctgt ttcaccgttg cggtcagttg ctccatgctg gctgccgtct cttccagcga
    10861 agcggcctgt tgctcggtgc gcgaagagag atcattattg ccggtagcga tttcgctggc
    10921 accgctatag atggcattgg ccccgttgcg cacatcaccg acggtacgca tcagctctcc
    10981 ctgcatatgg cgcaaactct ctgccagttg ccccatctca ttagagccat ccacctcaat
    11041 cggtttcacc agatcgccgc ctgcaatatg acgaatgctg tcaatcaggc gattcattgg
    11101 cgctaccagc gaggctttaa taccgaacca gacggcgaag atgaccgcca gtacgacgat
    11161 catcacgccc accagaatcc acatcgcctg gctgtaggag gcattgttat cgctgacggc
    11221 gatatcatag agccgatcgt tttgctccat gtaagccaca tactgcttct cgaaaccgtc
    11281 ctgatatccc tgggtcggct gatcaaagaa ctcgttgatt ttgcctgcac ctaacagttg
    11341 gatcagctcc gccagcgcat tgtgataaat atcgtaatta cgtttgatct ctgccgctgc
    11401 ggcggtgctc tgacgcgggt cacgcggcaa cgcttcgtaa tccgcccagt ttttttccgc
    11461 ctgtttcagc gaaatactgg cactctgcat cagctcagca acggttgaac cgctaccaat
    11521 attattctga tccatcatgt agcggatacc cgcgcggttg agggtgttac gcgtctgcaa
    11581 caacgcgacc cagctgccat tcagcgtggg ttgctgctgg cgaatggttt gtaaaacagt
    11641 gaaattttct ttgtcattct ttaaggcatt aaaaaacaga ccgcctgatg tcagttgtaa
    11701 aaggccaaaa acggccaaaa ccagcagtaa gctggtcacg atcttgatac gttttaacat
    11761 ggtttctctt tcctgtggac cgatgcaaga ttttcggcct ggaaagcaaa aactttatga
    11821 aaaggtgtaa cccgccatcc atggaaaaac caggcgctgg ttatcagata ctaaaaagtt
    11881 attcattatc gcccgggatg tcttcttgtc gggaattgcc cagagcaata aattcctcta
    11941 acagatgggt taactgttgc attttctcgg cagtaaactg agcttcaatc tgccgatagg
    12001 cttcttcaat ctgtgtctga gcgcggttat acagcgcctg cccctctttg gtcagcgaga
    12061 tatagagctt ccgctgatca ttgatcggct ttaatcgcaa cactaaaccg tcgcgttcca
    12121 tccgcgtgag gatcccggtc aggcttgggc gcaaaatgca ggcgcgatac gccagatcgt
    12181 gaaaatccat tgatgggctt tccgccagaa tacgcacgat gcgccactgt tgctcagtca
    12241 aattatgccg tttaacgatt gggcgaaaat aactcatcgc cgcttctcgc gcctggagca
    12301 acgcaatggt tagtgagtcg tgcattatct ttcccctgaa aaattcaaaa tcattaatat
    12361 agaaacaatt aatcatcacg ttaccgggac tgagctgccc ccgcaattct tcattttatc
    12421 aaaattgatg atattttatt aacttactga tatttaatat tttattatct ttaaatgtaa
    12481 atagtttgtt aattagatca catttatatc acttactttt gccaaactac gcaatcggga
    12541 ataaaagtaa tcattaacat attaatgatt aagatgatcg aatccgagga gtggtaatga
    12601 aaggcactat cttcgccgta gcgttgaacc atcgcagcca acttgatgcc tggcaggaag
    12661 cgttccagca atctccctac aaagccccgc ctaaaactgc ggtctggttt attaaaccgc
    12721 gcaatacggt gattggttgc ggtgaaccga ttccctttcc acagggtgaa aaagtactga
    12781 gcggtgcgac tgttgcgctg attgtgggga aaacggcgac gaaagtacgt gaagaagatg
    12841 cggcagagta catcgccgga tatgcgctgg ataacgacgt cagcctgccg gaagagagct
    12901 tttaccgtcc ggcaatcaaa gcaaaatgtc gtgatggatt ctgccccatt ggcgaaaccg
    12961 tggctctcag caatgtcgat aatctgacta tctataccga gatcaacggg cgtcctgccg
    13021 atcactggaa taccgccgat ttacaacgta acgccgcaca gctgctgagc gccctgagcg
    13081 aatttgccac gctgaatcca ggcgatgcta ttctgctcgg cacgccacag gcgcgcgtgg
    13141 aaatacagcc aagcgatcgc gtgcgtgttc tcgcagaagg tttcccgccg ctggaaaatc
    13201 cggtagtgga cgaacgtgaa gtgaccacgc gcaagagctt cccaacgctg ccacacccgc
    13261 acggtacgct gtttgccctc ggcctgaact acgccgacca cgccagcgaa ctggaattta
    13321 agccaccgga agagccgctg gtgttcctga aagcgccgaa taccctcact ggcgacaacc
    13381 agacctccgt gcgcccgaac aatattgaat acatgcacta cgaagcggag ctggtggtag
    13441 ttattggcaa gcaggcgcgt aacgtcagcg aagccgatgc catggattat gtcgcgggct
    13501 acaccgtgtg taacgactac gccattcgcg actatctgga aaactactac cgccctaacc
    13561 tgcgggtcaa aagccgcgac ggactgacgc cgatgctttc aaccatcgtg ccgaaagagg
    13621 cgatcccgga cccgcataat ctgacccttc gcaccttcgt caacggcgag ttacgccagc
    13681 aaggcaccac cgccgatctg atcttcagcg tgcccttcct gatcgcctac ttaagcgaat
    13741 ttatgaccct gaatccgggc gacatgatcg ccaccggcac accaaaaggc ttatctgacg
    13801 tggtgcctgg cgatgaagta gtggtggaag tagaaggcgt gggccgcctg gtgaaccgaa
    13861 ttgtgagtga ggaaacagcg aaatgaaaaa agtaaatcat tggatcaacg gcaaaaatgt
    13921 tgcaggtaac gactacttcc agactaccaa tccggcaacg ggtgaagtgc tggcggatgt
    13981 ggcctctggc ggtgaagcgg agatcaatca ggcggtagcg gcagcgaaag aggcgttccc
    14041 gaaatgggct gatctgccga tgaaagagcg tgcgcgcctg atgcgccgtc tgggcgatct
    14101 gatcgaccag aacgtgcctg agatcgccgc gatggaaacc gcggacacgg gcctgccgat
    14161 ccatcagacc aaaaatgtgt tgatcccacg cgcttctcac aactttgaat ttttcgcgga
    14221 agtctgccag cagatgaacg gcaagaccta tccggttgac gacaagatgc tcaactacac
    14281 gctggtgcaa ccggtaggcg tttgtgcgct ggcgtcgccg tggaacgtgc cgtttatgac
    14341 cgccacctgg aaggtcgcgc cgtgtctggc gctgggcaat accgcggtgc tgaagatgtc
    14401 ggaactctcc ccgctgaccg ccgaccgcct gggtgagctg gcgctggaag ctggtattcc
    14461 ggcgggcgtg ctaaacgtgg tacagggcta cggcgcaacc gcaggcgatg cgctggtccg
    14521 tcatcatgac gtgcgtgccg tgtcgttcac cggcggtacc gccaccgggc gcaacatcat
    14581 gaaaaacgcc gggctgaaaa aatactccat ggagctgggc ggtaaatcac cggtgctgat
    14641 ttttgaagat gccgatattg agcgtgcgct ggacgccgcc ctgttcacca tcttctcaat
    14701 caacggcgaa cgctgcaccg ccggttcgcg catctttatt cagcagagca tctatccaga
    14761 attcgttaaa cgctttgccg aacgcgccaa ccgtctgcgc gtgggcgatc cgaacgatcc
    14821 gaatacccag gttagggcgc ttatcagtca gcaacactgg gaaaaagtct ccggctatat
    14881 ccgtctcggc attgaagaag gcgcaaccct gttggcgggc ggcccggata aaccgtccga
    14941 cctgcctgcg cacctgaaag gcggcaactt cctgcgccca acggtgctgg cggacgtaga
    15001 taaccgtatg cgcgttgccc aggaagagat tttcgggccg gtcgcctgcc tgctgccgtt
    15061 taaagacgaa gccgaaggct tacgcctggc aaacgacgtg gagtacggcc tcgcgtcgta
    15121 catctggaca caggatgtca gcaaagtgtt acgcctggcg cgtggcattg aagcaggcat
    15181 ggtgttcgtc aacacccaga acgtgcgcga cctgcgccag ccatttggcg gcgtaaaagc
    15241 ctccggcacc gggcgtgaag gcggtgagta cagcttcgaa gtgttcgcgg aaatgaagaa
    15301 cgtctgcatt tccatgggcg accatccaat tccgaaatgg ggagtctgat atgggtaagt
    15361 tagcgttagc cgccaagatc acgcacgtac cgtcgatgta tctctctgaa ctaccaggaa
    15421 aaaaccacgg ttgtcgccag ggtgcgatcg acgggcataa agagatcagc aagcgttgcc
    15481 gggaaatggg cgtcgatacc attatcgttt tcgatactca ctggctggtc aacagtgctt
    15541 atcacatcaa ctgtgcagac cattttgaag gcgtctacac cagcaacgag ctgccgcatt
    15601 ttattcgcga catgacctac aactacgagg gcaacccgga gttggggcag ctaattgccg
    15661 aggaagcctt aaagctcggc gtgcgggcaa aagcgcacaa tattcccagc ctgaaactgg
    15721 aatacggcac gctggtaccg atgcgctaca tgaatgaaga caagcacttc aaagtggtct
    15781 ccatttcggc tttctgcacg gttcacgatt ttgccgacag ccgcaagctg ggcgaagcga
    15841 ttctgaaagc gatcgaacag tacgacggca ccgtggcggt ccttgccagc ggttcgttat
    15901 cgcaccgctt tattgacgat cagcgtgcgg aagaagggat gaacagctac acccgcgagt
    15961 tcgaccgcca gatggacgag cgtgtggtga agctgtggcg cgaaggccag ttcaaagagt
    16021 tttgcaatat gctgccggag tacgccgact actgctacgg cgaaggcaat atgcacgaca
    16081 cggtgatgct gctggggatg ctcggctggg ataaatacga cggcaaggtg gagtttatta
    16141 ccgagctgtt cccaagctct ggcaccggtc aggttaacgc tgttttcccg ctgcccgcgt
    16201 aaggacgttt tatgccgcac tttatcgtgg aatgtactga aaatattcgt gaagaagccc
    16261 gcttacctga gctgtttgcc agtgttaaca ctgcactggc ggcaacgggg atttttccgc
    16321 ttgggggaat acgtagccgg gcgcactgga tcgatacctg gcaaatggct gatggcaagc
    16381 acgattacgc ctttgtgcat atgacgctaa aaatcggcag cggacgcagt ctggaaagcc
    16441 gtcaggaagt gggcgaaatg ctgtttgatc tgattaaaac gcacttcgcc tcactgatgg
    16501 agagccgtta cctagcgctt tcatttgaga ttgcagagct gcacccgacg ttgaatttca
    16561 aacagaacaa cgtacacgcg ttgtttaaat aaatttgcac cagcttccag gccggatgat
    16621 aaggcgctgc cgcatccggc aatgccacag gatatcgcta tgttcgataa acacacccac
    16681 accctgatcg cccagcgtct ggatcaggca gaaaaacagc gcgaacagat ccgcgcgatc
    16741 tcgctggatt acccggagat caccatcgaa gacgcctacg ccgtgcagcg cgaatgggtt
    16801 cgcctgaaaa tcgccgaaag tcgcacgctg aaaggccaca aaatcggtct gacctcgaaa
    16861 gcgatgcagg caagctcgca gatcagcgaa ccggattacg gtgcactgct ggacgacatg
    16921 ttcttccacg atggcagcga tatcccgacc gatcgcttta tcgtgccgcg cattgaagtg
    16981 gagctggctt ttgtgctggc aaaaccgctg cgtgggccaa actgcacgct gttcgacgtt
    17041 tacaacgcca cggactatgt gatcccggcg ctggagctga tcgacgctcg ctgccacaac
    17101 atcgatccgg aaacccagcg cccgcgcaaa gtattcgaca ccatttccga taacgccgcc
    17161 aacgccggag tgatcctcgg aggtcgtccg atcaaaccag acgaactgga tctgcgttgg
    17221 atctccgccc tgatgtatcg caatggcgtg attgaagaaa ccggcgtcgc cgctggtgtg
    17281 ctgaatcatc cggcaaacgg cgtggcctgg ctggcgaaca aactcgcacc gtatgacgta
    17341 caactggaaa ccgggcaaat cattctcggt ggttcgttca ccctcccggt tccggcgcgt
    17401 aagggcgaca ccttccacgt cgattacggc aacatgggct ccattagctg ccgctttgtt
    17461 taaggagaga acgatggaaa acagttttaa agcggcgctg aaagcaggcc gtccgcagat
    17521 tggattatgg ctggggctga gcagcagcta cagcgcggag ttactggccg gaccaggatt
    17581 cgactggtta ttgatcgacg gtgagcacgc gccgaataac gtgcaaaccg tgctcaccca
    17641 actacaggcg attgcgccct accccagcca gccagtggta cgcccatcgt ggaacgatcc
    17701 ggtgcaaatc aaacaactgc tggacgtagg cacacaaacc ttactggtgc cgatggtaca
    17761 aaacgccgac gaagcccgta aagcggtacg cgccacccgt tatccccccg ccggtattcg
    17821 cggtgtgggc agtgcgctgg ctcgcgcctc gcgctggaat cgcattcctg attacctgca
    17881 aaaagccaac gatcaaatgt gcgtgctggt gcagatcgaa acgcgtgagg caatgaagaa
    17941 cttaccgcag attctggacg tggaaggggt cgacggcgtg tttatcggcc cggcggatct
    18001 gagcgccgat atgggttatg ccggtaatcc gcagcacccg gaagtacagg ccgccattga
    18061 gcaggcgatc gtgcagatcc gcgaagcggg caaagcgccg gggatcctga tcgccaatga
    18121 gcaactggca aaacgctatc tggaactggg cgcgctgttt gtcgccgtcg gcgttgacac
    18181 caccctgctc gcccgcgccg ccgaagcgct ggcagcacgg tttggtaata gttcttctat
    18241 ttcgatggcg aagcaaaaca acaactctgt ctactaaacg tacgatgtgg agcgcatcat
    18301 gacgacctct accctgcaat ataaagataa taaaactgtt gaaattgaac atcgtgtcat
    18361 taataaatta tttcgtcgcc taatcatttt tctttttatt ctgtttgttt tttcgtttct
    18421 tgatcgcatc aatataggat ttgccggact gacaatgggc aaggatcttg gcctgacgtc
    18481 cacaatgttt ggcctcgccg caacactctt ttatgtcacg tacgtcatgt gcgggatccc
    18541 cagtaatatg atgttagcga aaattggcgc tcgtcgatgg atcgctggga tcatggtggt
    18601 ctggggaatt gcttccacct gcacaatgtt tgcaacaagc cccgaaacac tctatctact
    18661 gcgtatgctg gttggtattg ctgaggcggg ttttttacct ggtattttgg tttatctgac
    18721 ctggtggttc ccggcatatc atcgggccag agccaatgcc ttatttatga tagcgatgcc
    18781 agtcactatg atgctgggat tgatcctttc aggctacatt ctggccttgg atggactgtg
    18841 gaatctaaaa ggctggcaat ggctcttttt gctggaaggt ctgccatcgg tcattcttgg
    18901 tgtcgtcacc tggttttatc tcaacgacac accagaccag gcgacctggc ttgatgaaaa
    18961 cgaaaaacag gctttaaaaa cgatgattct ccgtgaacaa gagagtgcat ttgcccatgc
    19021 atctgttcca cgctcaacct tacgtgaagt cctgacccca gccatcatgc tatatacact
    19081 tgcttatttt tgcctgacta atactctgag tgcgattaat atctggacgc cgcaaatcct
    19141 acagagtttc aatactggca gtagcaacat catgattggt ttgctggcgg ccatccccca
    19201 attctgcaca atttttggca tgatctggtg gagtcgtcgt tccgaccgtc ttaaagaacg
    19261 caaaaagcat acgattcttc cctacctgtt tgccgctgcc ggatggttac tcgcttctgc
    19321 gacagatcac agcctcattc aactgagcag catcattatg gcctcaatgg gatcatttac
    19381 cgcgatgact atcttttgga caacaccgga tcaagttata agcttgcaat cgcgtgccgt
    19441 tgcgatggct gtcatcaatg ctgtcggcaa tgtaggttca gcagtcagtc cattacttat
    19501 cggtattttg aaagatgcaa ccggcagttt tagctcaggt ttgtggtttg tagcaggatt
    19561 gctggtactg ggcgcgttag tcatgacccg aatccctatg gggagtcggg aacactctct
    19621 ttcatctgaa cttatcgcac caaaaccgca ataaggagtc atgatgtcca gtgaagctat
    19681 ctttgaacat ggttatgaaa atattgattt ggtccgggaa tatgatgccc gctacgctgg
    19741 tgaagatgtg cattatgaaa catttgcccg gttagcgaat ttctttggac gcgatatgcg
    19801 ccctcactgg cacgatagat attttcaact ccattatctg gtcactggac gcattacttt
    19861 acaactggat gagcacttct attctgtgcg cgcaccgctt tttgtactaa caccaccatc
    19921 ggtgccacat actttcttta ctgatcctga tagcgatggg catgttctga ccgtacgtca
    19981 ggagttaatt tggcccctgg tagaaaagct ctggcctggt agtggagatg ccgcaatgct
    20041 acaaggtatc tgtctgtcac tggagggaat ggaacataca ctggcggcat taaatcatta
    20101 ttggccgata atatccctgg agtttcaaca aaataagcaa ggacgggaaa ttctattgca
    20161 atcactggcg caaagtattt ttacaatcct actgcgtgaa gcaccaccaa atgatatttc
    20221 aacatgcagc gtgcgcggcg agatgtgttt attccagaag tttaatcggc tgattgacga
    20281 aaattatcgc caacatttaa tggtccctga atatgcaaca atgctcggta tgagtgaatc
    20341 acggcttacc gagctgtgcc gacgttttgc caatcaatcg ccgaagagat tgattttcga
    20401 acgagtgagc cgggaagcca gacggcttct gctctatagc gagcaaagca tcaaccagat
    20461 agcccttgat cttggctaca aagatcccgc ctatttcgcc cgttttttca accgaatgat
    20521 gggttgctca ccaacacaat tccgtggtcg ctgaccattt atcaacctga gagactcgac
    20581 atgaaaatat ctttacgctc actatttgtg gcgtgcctga ttgcgccagt ggcattcacc
    20641 tacgcctccg aaccgcaata aatgaaaatt gtactacttg gaacgaaagg tggcccttct
    20701 ttattaaaag ctgaccgttt accgatggca acggcactta ttattggcaa taaaacctgg
    20761 ttaattgatg ctggttacgg ggccagctta caactggtaa aaaataatat tccactgcgc
    20821 cagattgata atattttact cacccattta cacagtgatc atattctgga ctatccatca
    20881 gtcttaatga atgcatgggc tagcggtctg caggacaaaa ctattcatgt attcggtccg
    20941 ccaggcaccc gagccatgac cgctgccagt tggcaggtct tcgatcggga catcacactg
    21001 cgtatggaag aagaaggaaa acccgatcct cgcaaactcg ttacagctca agatgttggt
    21061 gaaggcgtag tttataaaga tgataccgtg accgtgactg cattaaaggt ccctcattct
    21121 cctttccctg acggtgaggc ttttgcttat cgcttcgaca cacaaggcaa gcgaattgtc
    21181 ttctcaggag atacctcgta ctttccccca ctggcaactt tctcagaagg cgctgatata
    21241 cttgtgcatg aggccgtaca tgagccctcc gtcgctaagc tggcagacag tattggcaat
    21301 ggtaaaacac tggcaaaagc cattgcttct caccatacga ctattgagga tgtaggcaaa
    21361 attgccaatg aagcaaaagt gaaaaaactc atccttagcc acctggtacc aatcaccgtt
    21421 ccggatgaaa catggaagca agaagcacag aaaactattc aggtcctatc gtcgttggtc
    21481 atgaaaatat gacgattacc attccattcg ccccataaaa aattccgtcc ccgttagccc
    21541 ttgttagcgg ggaatattat gatttgcctt actgtaagtt gatcacgatc gcagtttact
    21601 atttaactcc accttgctgt cgaaaagtac caacgttcgc ctgaaacgtc tcttcccgac
    21661 aaccgcgttc aacgcttcaa ttaaataaca aaaacaacac acaaaattaa caatcgtatt
    21721 ccgattaata ctgtagaggt cgacatgaaa ccagaagatt tccgcgccag tacccaacgt
    21781 cctttcaccg gggaagagta tctgaaaagc ctgcaggatg gtcgcgagat ctatatctat
    21841 ggcgagcgag tgaaagacgt caccactcat ccggcatttc gtaatgcggc agcgtctgtt
    21901 gcccaactgt acgacgcgct gcacaaaccg gagatgcagg actctctgtg ttggaacacc
    21961 gacaccggca gcggcggcta tacccataaa ttcttccgcg tggcgaaaag tgccgacgac
    22021 ctgcgccagc aacgcgacgc catcgctgaa tggtcacgcc tgagctatgg ctggatgggc
    22081 cgtaccccag actacaaagc cgctttcggt tgcgcactgg gcgcgaatcc gggcttttac
    22141 ggtcagttcg agcagaacgc ccgtaactgg tatacccgta ttcaggaaac tggcctctac
    22201 tttaaccacg cgattgttaa cccaccgatc gtcatttgcc gaccgataaa gtgaaagacg
    22261 tttacatcaa gctggaaaaa gagactgacg ccgggattat cgtcagcggt gcgaaagtgg
    22321 ttgccaccaa ctcggcgctg actcactaca acatgattgg cttcggctcg gcacaagtga
    22381 tgggcgaaaa cccggacttc gcactgatgt tcgttgcgcc aatggatgcc gatggcgtga
    22441 aattaatctc ccgcgcctct tatgagatgg tcgcgggtgc taccggctcg ccatacgact
    22501 acccgctctc cagccgcttc gatgagaacg atgcgattct ggtgatggat aacgtactga
    22561 tcccatggga aaacgtgctg atctaccgcg attttgatcg ctgccgtcgc tggacgatgg
    22621 aaggcggttt cgcccgtatg tatccgctgc aagcctgtgt gcgcctggca gtgaaactcg
    22681 acttcattac ggcactgctg aaaaaatcac tcgaatgtac cggcaccctg gagttccgtg
    22741 gtgtgcaggc cgatctcggt gaagtggtgg cgtggcgcaa caccttctgg gcattgagtg
    22801 actcgatgtg ttcagaagcg acgccgtggg tcaacggggc ttatttaccg gatcatgccg
    22861 cactgcaaac ctatcgcgta ctggcaccaa tggcctacgc gaagatcaaa aacattatag
    22921 aacgcaacgt taccagtggc ctgatctacc tcccttccag tgcccgtgac ctgaacaatc
    22981 cgcagatcga ccagtatctg gcgaagtatg tgcgcggttc gaacggtatg gatcacgtcc
    23041 agcgcatcaa gatcctcaaa ctgatgtggg acgccattgg cagcgaattt ggtggtcgtc
    23101 acgagctgta tgaaatcaac tactccggta gccaggatga gattcgcctg caatgtctgc
    23161 gccaggcaca aagctccggc aatatggaca agatgatggc gatggttgat cgctgcctgt
    23221 cggaatacga ccagaacggc tggactgtgc cgcacctgca caacaacgac gatatcaaca
    23281 tgctggataa gctgctgaaa taacgcagca ggaggttaag atgcaattag atgaacaacg
    23341 cctgcgcttt catgacgcga tggccagcct gtcggcagcg gtaaatatta tcaccaccga
    23401 gggcgacgcc ggacaatgcg ggattacggc aacggctgtc tgctcggtca cggatacacc
    23461 accgtcgctg atggtgtgca ttaacgccaa cagtgcgatg aacccggttt ttcagggcaa
    23521 cggcaagttg tgcgtcaacg tcctcaacca tgagcaggaa ctgatggcgc gccacttcgc
    23581 gggcatgaca ggcatggcga tggaagagcg ttttagcctc tcatgctggc aaaaaggtcc
    23641 gctggcgcag ccggtgctaa aaggttcgct ggccagtctt gaaggagaga tccgcgatgt
    23701 gcaggcgatt ggcacacatc tggtgtatct ggtggagatt aaaaacatca tcctcagtgc
    23761 agaaggtcac ggacttattt actttaaacg ccgtttccat ccggtgatgc tggaaatgga
    23821 agctgcgatt taagctccag ccccttcacg cattcgtcga aggggcattt ttattgtgat
    23881 ctcaatcaca tatctacccg atgaataatt cttgcccaac cgctcaccgc cattcctgac
    23941 cgcttacatc cctaaaataa ccactcagtt atttacctta ctttacgcgc acgtatctct
    24001 ggcaacatca ctgcaggata gcggtcaatt tacctcctca aacgcaacgc aaacctagaa
    24061 cggcttcggc ctatgattaa caaattactc tatggatttt ggtttgcagg aaggcagcaa
    24121 gtgagtgaat ccccgggagc ttacaaaagt aagtgtcaga ggtgaacgaa cgcagctgcc
    24181 gcacctgtaa gccaaaagac gacgagtaaa atgccaggtt ttactatgga tacgaaaaaa
    24241 ctattcaagc acataccctg ggtgattctc ggaatcatcg gtgcattctg cctcgcggta
    24301 gttgcattac gtcgggggga gcacgtcagc gccctgtgga tcgtggtcgc ctctgtgtcg
    24361 gtgtatctgg tggcgtatcg ctactacagt ctgtacatcg cccagaaggt gatgaaactc
    24421 gaccccacgc gcgcgacgcc tgcggttatt aacaacgacg gtctgaacta cgttccgacc
    24481 aaccgttacg tgttgtttgg tcaccacttc gccgctatcg ccggtgctgg tccgctggtg
    24541 ggaccggttc tcgccgcgca gatgggctac ctgcctggca cgctgtggct gctggcgggg
    24601 gtagtgctgg ccggtgcggt acaggacttt atggtgctgt ttatctcctc tcgccgtaac
    24661 ggcgcatctc tcggtgagat gatcaaagaa gagatggggc cagtaccggg gactatcgcg
    24721 ctgtttggct gtttcttaat catgatcatc atcctcgccg ttctggcgct gattgtggtt
    24781 aaagccctgg ccgaaagtcc gtggggtgtc ttcaccgttt gctcaaccgt accgattgcg
    24841 ctgtttatgg gtatctacat gcgctttatc cgtccggggc gtgtgggtga agtctctgtt
    24901 attggtatcg tgctgctggt tgcctctatc tacttcggtg gcgtgattgc tcacgatccg
    24961 tactggggtc cggcactgac ctttaaagac accaccatta ccttcgcgct gattggctat
    25021 gcgtttgttt ccgcactgct gccggtgtgg ctgatcctcg caccgcgcga ctatctggca
    25081 accttcctga aaatcggcgt tatcgtcggc ctggcgctgg gtatcgtggt gctgaacccg
    25141 gaactgaaaa tgcctgcgat gacccagtac attgacggta ctggcccgct gtggaaaggc
    25201 gctctgttcc cgttcctgtt catcaccatc gcctgtggtg cggtatctgg cttccacgcg
    25261 ctgatctctt ccggtacgac gccgaaactg ctggctaacg aaaccgacgc gcgtttcatt
    25321 ggctacggcg caatgctgat ggagtccttc gtggcgatta tggcgctggt tgcagcgtcc
    25381 atcatcgaac cgggtcttta cttcgcgatg aacaccccgc ctgctggcct tggcatcacc
    25441 atgcctaacc tgcatgaaat gggtggcgag aacgcgccga tcatcatggc gcagctgaaa
    25501 gacgttaccg cacacgcggc agcgaccgtc agctcctggg gcttcgtgat ttcgccagag
    25561 cagatcctgc aaaccgcgaa agacattggt gagccttctg tcctgaaccg tgcaggtggt
    25621 gcgccgacgc tggcggtagg tatcgctcac gtgttccaca aagtgctgcc gatggctgac
    25681 atgggcttct ggtatcactt cggtattctg ttcgaagccc tgttcatcct gaccgcgctg
    25741 gatgcgggta cccgttctgg ccgctttatg ctgcaagacc tgctgggtaa cttcatcccg
    25801 ttcctgaaaa aaaccgactc tctggttgcc ggtatcatcg gtactgcggg ctgtgtgggt
    25861 ctgtggggct acctgctgta tcagggcgtg gtcgatccgc tgggcggcgt taagagcctg
    25921 tggccgctgt tcggtatctc caaccagatg ctggcagccg tagcgctggt actgggcacc
    25981 gttgtgctga ttaagatgaa gcgcacccaa tacatctggg taactgttgt tccggctgta
    26041 tggctgctta tctgcaccac ctgggcgctg ggtctgaaac tgttcagcac caacccgcag
    26101 atggaaggct tcttctacat ggcaagccag tacaaagaga agattgctaa cggtactgac
    26161 ctgacggcgc agcagattgc caacatgaac cacatcgttg tgaacaacta caccaacgca
    26221 ggcctgagta ttctgttcct gattgtggtg tacagcatca tcttctacgg tttcaaaacc
    26281 tggcttgcgg tgcgtaacag cgacaaacgt actgacaaag aaacaccgta cgttccaatc
    26341 ccggaaggcg gcgtgaagat ctcttcgcac cactaaccgt gtttagcccc gcttcggcgg
    26401 ggctttgttc ttatcagagt gaactatgtt tggtaactta ggacaggcaa aaaaatatct
    26461 cggccaggcg gcgaagatgt tgattggtat tccagactac gacaactacg tcgagcatat
    26521 gaagaccaac catcccgaca agccgtacat gagctatgaa gaattcttcc gcgaacgcca
    26581 gaacgcgcgc tacggcggcg atggtaaagg cggtatgcgc tgttgttaat ggagagagca
    26641 tgaacccgat tgcagttacc ctactcaccg gttttttagg cgcgggcaaa actaccctgc
    26701 tgcgccatat tcttaacgaa caacacggct acaagattgc cgtgattgaa aacgaattcg
    26761 gcgaagtctc cgttgatgac caactgattg gcgaccgcgc cacgcagatc aaaacgctga
    26821 ccaacggctg catctgctgt tcgcgctcca acgagctgga ggacgcacta ctcgacctgc
    26881 tggataatct cgacaagggc aatattcagt tcgatcgtct ggtgattgaa tgcaccggca
    26941 tggccgatcc cggcccgatt attcagacct ttttctccca tgagatttta tgccagctct
    27001 acctgctgga cggcgtgatt gcgctggtgg atgcggtaca tgccgatgag cagatgaacc
    27061 agttcaccat cgcccagtcc caggtgggct acgccgaccg tattctgctg actaaaaccg
    27121 acgtcgcagg cgaagcagag aaactgcgtg aacgcctggc gcgcatcaac gcccgcgcac
    27181 cggtctacac cgtcacccac ggcgacatcg acctgggcct gctgttcaac accaacggtt
    27241 ttatgctgga agagaacgtc gtcagcacca aaccgcgttt ccactttatc gcggataaac
    27301 aaaacgatat ttcgtcaatt gtggtggaac tggattaccc ggtagatatc agcgaagttt
    27361 cccgcgtgat ggaaaacctg ctgctggaat cggcggataa actgctgcgt tacaaaggga
    27421 tgctgtggat tgacggcgaa cctaaccgcc tgctgttcca gggcgtccag cgcctctaca
    27481 gcgccgactg ggacaggccg tggggcgatg aaaaaccgca tagcacaatg gtgtttatcg
    27541 ggattcagtt gccggaggaa gagattcggg cggcgtttgc gggattgagg aagtaagttc
    27601 gggaaagatc aactttttga gagttccacc gcaacattat tacgagtgga actctttaga
    27661 tatgtcagac caaccaaagc ttcttcaatt gcgttttcat tttttcatgt ctcattttca
    27721 gttcagccaa tccccattct gaagaattac aaacatcttt tgttaaataa aaagagcttt
    27781 tactattaag tttctcataa atagatttct ttttgataaa atcatagtgt tgtgtttcag
    27841 aatttttgga accacttatg agtgttaaat tacctatttt atgcagccag tagacatgct
    27901 cttgaggttg gaatctatta atccagtatt cattaactaa tgcttgagga agaatatgct
    27961 caattgtgat tctccccgta taggttttaa tcacgctctc atcttgttgc tccatatcta
    28021 agcgaagcaa aattgctttt attaagttca cttgattcgg acgaggctca tataaatctt
    28081 catccagagc agctataaaa cctgaattat ctgcatgttg gtttatctga tttatcacag
    28141 aatcaaaagg catatcatta tttattgcta ccagagcaga ataacaaacc atttctcttt
    28201 ggcttttaat ttgttttttt aaccacccat gcatatatac cttctcaaag gcggtaataa
    28261 attgagaaaa atcatctaaa ttaaaatctt ctgtacgagc cattcgattc atgaatgcca
    28321 taaccggagg aatccactca tctaccccta aattacttag cgatgctatt tttcgtcgta
    28381 ttgaagggtg ttcaaaatca ttctctaaaa tcttagtata
//
LOCUS       DAJTSA010000049        28402 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000049 DAJTSA010000000
VERSION     DAJTSA010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.049"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="OO369_002249"
     CDS             complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="OO369_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HCR5982508.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            321..1952
                     /gene="dcuS"
                     /locus_tag="OO369_002250"
     CDS             321..1952
                     /gene="dcuS"
                     /locus_tag="OO369_002250"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR5982509.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1949..2668
                     /gene="dcuR"
                     /locus_tag="OO369_002251"
     CDS             1949..2668
                     /gene="dcuR"
                     /locus_tag="OO369_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR5982510.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3239..4579
                     /gene="dcuB"
                     /locus_tag="OO369_002252"
     CDS             3239..4579
                     /gene="dcuB"
                     /locus_tag="OO369_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR5982511.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4657..6303
                     /gene="fumB"
                     /locus_tag="OO369_002253"
     CDS             4657..6303
                     /gene="fumB"
                     /locus_tag="OO369_002253"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5982512.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6426..7055
                     /locus_tag="OO369_002254"
     CDS             6426..7055
                     /locus_tag="OO369_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR5982513.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7194..8615)
                     /gene="melB"
                     /locus_tag="OO369_002255"
     CDS             complement(7194..8615)
                     /gene="melB"
                     /locus_tag="OO369_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR5982514.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8718..10073)
                     /gene="melA"
                     /locus_tag="OO369_002256"
     CDS             complement(8718..10073)
                     /gene="melA"
                     /locus_tag="OO369_002256"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR5982515.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            10356..11264
                     /gene="melR"
                     /locus_tag="OO369_002257"
     CDS             10356..11264
                     /gene="melR"
                     /locus_tag="OO369_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR5982516.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11463..13730
                     /gene="adiA"
                     /locus_tag="OO369_002258"
     CDS             11463..13730
                     /gene="adiA"
                     /locus_tag="OO369_002258"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR5982517.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14055..14816
                     /gene="adiY"
                     /locus_tag="OO369_002259"
     CDS             14055..14816
                     /gene="adiY"
                     /locus_tag="OO369_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR5982518.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14953..16290
                     /gene="adiC"
                     /locus_tag="OO369_002260"
     CDS             14953..16290
                     /gene="adiC"
                     /locus_tag="OO369_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR5982519.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16394..18037
                     /gene="eptA"
                     /locus_tag="OO369_002261"
     CDS             16394..18037
                     /gene="eptA"
                     /locus_tag="OO369_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR5982520.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18034..18702
                     /gene="basR"
                     /locus_tag="OO369_002262"
     CDS             18034..18702
                     /gene="basR"
                     /locus_tag="OO369_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR5982521.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18703..19803
                     /gene="pmrB"
                     /locus_tag="OO369_002263"
     CDS             18703..19803
                     /gene="pmrB"
                     /locus_tag="OO369_002263"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR5982522.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19779..19868)
                     /gene="pmrR"
                     /locus_tag="OO369_002264"
     CDS             complement(19779..19868)
                     /gene="pmrR"
                     /locus_tag="OO369_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR5982523.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19980..21482)
                     /gene="proP"
                     /locus_tag="OO369_002265"
     CDS             complement(19980..21482)
                     /gene="proP"
                     /locus_tag="OO369_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR5982524.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21746..22624)
                     /locus_tag="OO369_002266"
     CDS             complement(21746..22624)
                     /locus_tag="OO369_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR5982525.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22621..24849)
                     /gene="crfC"
                     /locus_tag="OO369_002267"
     CDS             complement(22621..24849)
                     /gene="crfC"
                     /locus_tag="OO369_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR5982526.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25252..25587
                     /locus_tag="OO369_002268"
     CDS             25252..25587
                     /locus_tag="OO369_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR5982527.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25745..26188
                     /gene="yjdN"
                     /locus_tag="OO369_002269"
     CDS             25745..26188
                     /gene="yjdN"
                     /locus_tag="OO369_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR5982528.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26321..27109
                     /gene="phnC"
                     /locus_tag="OO369_002270"
     CDS             26321..27109
                     /gene="phnC"
                     /locus_tag="OO369_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR5982529.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27134..28150
                     /gene="phnD"
                     /locus_tag="OO369_002271"
     CDS             27134..28150
                     /gene="phnD"
                     /locus_tag="OO369_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5982530.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            28256..>28402
                     /locus_tag="OO369_002272"
     CDS             28256..>28402
                     /locus_tag="OO369_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HCR5982531.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMAT"
BASE COUNT         7296 a         7417 c         6935 g         6754 t
ORIGIN      
        1 gactttcgtt tgagattaaa taacttgccg ttgccacgta cgcaattgcc agaatgctga
       61 catatcgcag tgttgaaata gacatcgatc ttttcgccgg tataacagcg ataacccccg
      121 tccagtagcg cctgatccat gaccttccct ctctcatcag tatgattatc gctataagca
      181 tagccccgga atcttcatac agcactggca gtttccgttg ccagagtctc ttcccctata
      241 tagccaatca tttttactcc ctttgaatta cccgcctcat cagagataat gcttaagaaa
      301 tttgtcacac aaggaagctg atgagacatt cattgcccta ccacatatta cgcaaacgtc
      361 cgatgaaatt gagtaccact gtgatcttaa tggtcagcgc ggtactgttc tcggtgctat
      421 tggtggtgca tctgatttac ttctcgcaaa tcagtgatat gacgcgagac gggctggcca
      481 acaaggcact ggcagtggcg cgtaccctcg ccgactcgcc ggaaatccgt cagggcttgc
      541 agaaaaaacc gcaggagagt ggcatccagg ccatcgcgga agccgtgcgc aaacgcaacg
      601 atctgctgtt tattgtcgtt accgatatgc aaagtcttcg ctactcacat cctgaagccc
      661 agcgtattgg tcagccattt aaaggtgatg acatccttaa tgcgctgaat ggcgaagaaa
      721 atgtcgctat caatcgcggt tttctggcgc aggctttacg cgtatttacc cccatctacg
      781 atgaaaatca taaacaaatt ggcgtggtgg cgatcggcct tgagttaagc cgcgtgaccc
      841 aacagatcaa tgacagtcgc tggagcatta tctggtcggt attatttggc atgctggtcg
      901 gactgattgg cacctgcatt ctggttaagg tactgaaaaa aatccttttc ggcctggaac
      961 cctacgaaat ctccacgctg tttgagcaac gccaggccat gttgcagtct atcaaagaag
     1021 gcgtcgttgc cgtggacgat cgcggcgagg tcacgctgat caacgatgcc gcacaagaat
     1081 tgctgaatta ccgtaagtcg caggacgatg agaaactgtc gacgctcagc cactcatggt
     1141 cacaggtggt agatgtctcg gaagtgttac gcgacggtac gccgcgccgc gacgaagaga
     1201 ttacgattaa agaccggcta ttactgatca acaccgttcc ggtgcgcagt aatggcgtta
     1261 tcatcggtgc catttcaacc ttcagggaca aaactgaagt tcgtaaactg atgcagcgac
     1321 tcgacggtct ggtcaactat gctgacgcac ttcgtgaacg atcccacgaa tttatgaata
     1381 aattgcatgt gattctcgga ttattgcatc tgaagagtta taagcagttg gaagattaca
     1441 ttctcaaaac agccaataac tatcaggaag agattggctc tctgctgggt aagatcaaat
     1501 ctccggttat cgctggtttt ttaatcagca agattaaccg cgcgaccgat ttaggccata
     1561 cgctgatttt aaacagtgaa agccagctgc cagacagcgg cagtgaggac caggtcgcga
     1621 cgctgattac cacattggga aatctgatag aaaacgcgct ggaagcatta gggccggaac
     1681 ccggtggcga aattagcgta acattgcact accgtcacgg ctggctgcac tgcgaagtta
     1741 acgatgatgg accggggatc gcacccgaca aaatcgatca catttttgac aaaggtgtct
     1801 cgacaaaagg aagcgagcga ggcgtcggtt tagcacttgt caaacaacag gtagaaaatc
     1861 tcggcggcag catcgccgtg gaatcggaac cggggatttt cacacaattt tttgtccaga
     1921 taccctggga cggggagagg tcgaacagat gatcaatgta ttaattatcg atgacgacgc
     1981 aatggtcgcg gagctgaatc gccgatacgt agcacaaatc ccaggctttc aatgctgtgg
     2041 aacagcctcg acgctggaga aagccaaaga gattatcttc aatagcgatg cgcctatcga
     2101 cctgatattg ctcgatatct atatgcaaaa agagaacggg ctcgatttac tgcctgtcct
     2161 gcataacgcg cgttgcaaaa gtgatgtgat tgtcatctcc tccgcagccg atgcggcaac
     2221 cattaaagat tcgctgcatt acggtgtcgt ggattacctg atcaaaccct tccaggcttc
     2281 ccgctttgaa gaggcgctca ccggctggcg gcaaaagaaa atggcgctgg aaaaacatca
     2341 gtattatgac caggccgagc ttgatcagct aatccacggc agcagctcca acgaacagga
     2401 tcctcgtcgc ttgccaaaag gcttaacgcc gcagacgtta cgcacgctgt gccagtggat
     2461 tgacgcgcat caggactatg agttttcaac cgacgagctg gctaacgagg ttaacatctc
     2521 gcgtgtttcc tgccgaaaat acctcatctg gctggtcaac tgccacatct tgttcaccag
     2581 tatccattat ggcgtcacgg ggcgtccggt ttaccgttac cgtattcagg cagaacacta
     2641 ctcactgctg aaacaatatt gccaataacg gatcgctggt tatctgtaag taataattat
     2701 taccgtaatg caaaataccc ctggcacaag ttacatcgtt aatcagtaaa acttgtgcca
     2761 gatcaaatat aattatccct ccatcatcgt taaaaattaa tatctcttca gatgaacggt
     2821 gtttttaatt tcaaaacgat aacaaaagtt aattaactat tatgtcaccc gcattatgtg
     2881 tatttttacc cacaaatggg tagatcagat taatctataa acctaatgat atctgccctg
     2941 agaacaaaaa atagaccgat aaatatcaat aagataacag caaacaaaac attaacatct
     3001 gcgcagtaca aactataaac ccatcgccag agagtttttc tctctgaaaa agccgcttat
     3061 cacagtgcat aaatttgccg ctgctttaat cagccaatat tcactgtgag gtatttgcta
     3121 aagccggtaa cgaccaaacg gatatttagt caggctctga aaacagttca tacaaaacag
     3181 aacgtgactg tgatctattc agcaaaaatt taaataggat tatcgcgagg gttcacacat
     3241 gttatttact atccaactta tcataatact aatatgtctg ttttatggtg ccagaaaggg
     3301 tggtatcgcg ctgggtttat taggcggtat cggtctggtc attctggtct tcgtcttcca
     3361 ccttcagcca ggtaaaccac cagttgatgt catgctggtt atcattgcgg tggtggcggc
     3421 atcggcgacc ttgcaagctt cgggcggtct tgatgtcatg ctgcaaattg ccgagaagct
     3481 gctgcgccgc aacccgaaat atgtctcaat tgtcgcgccg tttgtgacct gtacactgac
     3541 cattctttgc ggtacgggtc atgtggttta caccattctg ccgatcatct acgacgtcgc
     3601 cattaagaac aacatccgtc cggaacgtcc gatggcggca agttctatcg gtgcacagat
     3661 ggggattatc gccagtccgg tgtcggttgc ggtcgtgtct ctggttgcga tgctgggtaa
     3721 tgtcaccttt gatggtcgcc atcttgagtt cctcgatctg ctggcaatca ccattccatc
     3781 gacgttaatc ggtatcctgg cgatcggtat cttcagctgg ttccgcggta aagatctgga
     3841 taaagacgaa gagttccaga aattcatctc cgtaccggaa aaccgtgagt atgtttacgg
     3901 tgataccgcg acgctgctgg ataaaaaact gccgaaaagc aactggctgg caatgtggat
     3961 tttcctcggg gcaatcgctg tagtcgccct tcttggtgct gattcggacc tgcgtccatc
     4021 cttcggcggc aaaccgctgt cgatggtact ggttattcag atgtttatgc tgctgaccgg
     4081 ggcgctgatt attatcctga ccaaaaccaa tcccgcgtct atctcaaaaa acgaagtctt
     4141 ccgttccggt atgatcgcca tcgtggcggt gtacggtatc gcatggatgg cagaaaccat
     4201 gttcggtgcg catatgtctg aaattcaggg cgtactgggt gaaatggtga aagagtatcc
     4261 gtgggcgtat gccattgttc tgctgctggt ttccaagttt gtaaactctc aggctgcggc
     4321 gctggcggcg attgttccgg tcgcgctggc gatcggcgtt gatccggcat acatcgtggc
     4381 ttcagcaccg gcttgctacg gttattacat cctgccgact tatccgagcg atctggcagc
     4441 gattcagttt gaccgttccg gcaccaccca catcggtcgc ttcgtcatca accacagttt
     4501 tattctgccg gggttgattg gtgtgagcgt atcgtgcgtc ttcggctgga tcttcgccgc
     4561 gatgtacggg ttcttataaa tgcactttgc gtgccgcccg tgactacgcg gcacgccatt
     4621 ttcgaataac aaatacagag ttacaggctg gaagctatgt caaacaaacc ctttatctac
     4681 caggcacctt tcccgatggg gaaagacaat accgaatact atctactcac ttccgattac
     4741 gttagcgttg ccgacttcga cggcgaaacc atcctgaaag tggaaccaga agccctgacc
     4801 ctgctggcgc agcaagcctt tcacgacgct tctttcatgc tacgtccggc acaccagaaa
     4861 caggttgcgg ctattcttca cgatccagaa gccagcgaaa acgacaagta cgtggcactg
     4921 caattcttaa gaaactccga aatcgccgcc aaaggcgtgc tgccgacctg ccaggatacc
     4981 ggcaccgcga tcatcgtcgg taaaaaaggc cagcgcgtgt ggaccggtgg cggtgatgaa
     5041 gaagcgctgt cgaaaggcgt ctataacacc tatatcgaag ataacctgcg ctactcgcaa
     5101 aacgcggcgc tggacatgta caaagaggtc aacaccggca ctaacctgcc tgcgcaaatc
     5161 gacctgtacg cggtagatgg cgatgagtac aaattccttt gcgttgcgaa aggcggcggc
     5221 tctgccaaca aaacgtatct ctaccaggaa accaaagccc tgctgactcc cggcaaactg
     5281 aaaaacttcc tcgtcgagaa aatgcgtacc ctcggtactg cagcctgccc gccgtaccat
     5341 atcgcgtttg tgattggcgg tacgtctgcg gaaaacaacc tgaaaaccgt caaattagca
     5401 agcgctcact attacgatga actgccgacg gaagggaacg aacatggtca ggcgttccgc
     5461 gatgtccagc tggaacagga actgctggaa gaggcccaga aactcggtct tggcgcgcag
     5521 tttggcggta aatacttcgc gcacgacatt cgcgttatcc gtctgccacg tcacggcgca
     5581 tcctgcccgg taggcatggg cgtctcctgc tccgctgacc gtaacattaa agcgaaaatc
     5641 aaccgcgaag gtatctggat cgaaaaactg gaacacaatc taggtcagta cattccacaa
     5701 gaactgcgtc aggccggtga aggcgaagcg gtgaaggtcg accttaaccg cccgatgaaa
     5761 gagatcctcg cccagctttc gcaatatccg gtttccactc gtttgtcgct cactggcacc
     5821 attattgtgg gtcgagatat tgcacacgcc aagctgaaag agctgattga cgccggtaaa
     5881 gaacttccgc agtacatcaa agatcacccg atctactacg cgggtccggc gaaaacccct
     5941 gccggttatc catcaggttc acttggccca accaccgcag gtcgtatgga ctcctatgtg
     6001 gatctgctgc aatcccacgg cggcagcatg atcatgctgg cgaaaggcaa ccgcagccag
     6061 caggttaccg acgcgtgtca taaacacggc ggcttctacc tcggtagcat cggcggtccg
     6121 gcggcggtac tggcgcagca gagcatcaag catctggaat gcgtcgctta tccggagctg
     6181 ggtatggaag ctatctggaa aatcgaagta gaagatttcc cggcgtttat cctggtcgat
     6241 gacaaaggta acgacttctt ccagcaaatc gtcaacaaac agtgcgcgaa ctgcactaag
     6301 taacctcttc ggcccagcgc ctggcagcat gctgccaggt gatccccctg ggccacctct
     6361 tttgcgattg taatttcatg cttgctggtg aatagtcagt attttccctg acttgcgaac
     6421 tcaccatgac ccgcacactc aagccgttaa ttcttaacac tggcgcactg gcgctaacgt
     6481 taatcctgat ttataccggc atttcggccc atgacaaact cacctggctg ctggaagtga
     6541 caccggtgat tatcgtcgtg ccgctactac ttgccaccgc cagacgttac ccgttaacgc
     6601 tgctgctcta tacgctcatc ttctttcacg ccatcatcct gatggtaggc gggcagtaca
     6661 cctacgcgaa agtccccgtt ggttttgagg tgcaggaatg gctggggttg agtcgtaatc
     6721 cgtatgacaa gctggggcac tttttccagg ggctggtgcc tgcactggtg gcacgagaaa
     6781 ttctcgttcg cgggatgtac gtgcgcggac gtaaaatggt ggcgtttctg gtctgttgcg
     6841 tggcgctggc gataagcgcg atgtatgaat taatcgagtg gtgggcggca ctggcgatgg
     6901 gccagggagc ggatgatttt ctcggtaccc agggcgacca gtgggacacg caatccgata
     6961 tgttctgcgc gctgcttggc gcattaacga cagtgatatt gctcgctcgc tttcattgcc
     7021 gccagctacg gcgctatggc ttgataactg gataacaacg tgcactttgt tgatgccgga
     7081 tgcggcgtga attccttatc cggtctacaa aatttgcaaa tccaaagcat tacattaaga
     7141 cgtggcctga taaacatatc gcatcaggcc attttcgtta gctattgtcc acgtcaggct
     7201 ttcacatcac tcactgcacc gaccggaata tcagcatgaa caggctcggg cggtactttg
     7261 cgatatttat ccagcagatg aatctggatc ctgcgcagcg tgtcgccatt gaggcgatag
     7321 aaacggaagt agagaatcag cgttaccatg aaaaacagag tcggtagagc aatcataata
     7381 aactgcatac ctaatagggc ttgcgtagac tgttcaacat tcggtacata gccaatcatc
     7441 cctaacacaa ccgcaatgaa aaaagccgca aaggctgaac cgcccttcac caccatagtc
     7501 tgcacggagt aagcgatgct ttcacagcgt acgtgcagtt tatattcacc gtaatcaacg
     7561 gtatctgcca ccatgatgac ctgtaatacc cagaaaagcg ccgttcccac attcagcaaa
     7621 atacccgcaa tcacaatgag gacgacgttg tgatagctca ttaatgccat taacaggaga
     7681 acaccacagc ttaacaccgg aagaatagat gctccggccc ataaaatgcg tcgggataat
     7741 gatttaacta agcgtgggaa gaatactaac gtcaccaggt tagcagctcc cgcatacgac
     7801 agataatagg ggaacaaatc cgcatcaccg ataacatatg agaaataata gatagcaaat
     7861 ccggtaataa tgttgctggc tacattataa gcaagagcca tacccaagag gcatgaaagc
     7921 tgatcgtttt tataaataag cgcaacgatg gctttaagtg tcagatggct tccttcagca
     7981 gacgattgat tgtctgacga aaagacttca tgcacattgc gcagagtgat gatggttgaa
     8041 acaataaaaa aggcgatcaa taccagagtg aacatctgaa agccaaatcc ccgatcgcca
     8101 ccgccgacat aattaacaaa tggtagcgtc acacctgccg taacaaagcc tgccagactg
     8161 gcaaaaaaac gcggataagg aaccagttgt tcgcgttcac gtttatcgag cgtgatggtt
     8221 ggaaccagcg accagaaggg aatatccata atggtgtaag tcatgcccca gaggatgtag
     8281 gtcacgcaaa caaagacaat ctgagtagta ccttcaaaca gatgcgcact aaagaggaga
     8341 aataagatta cagagtttgc caacgtaccg atcaggatcc agggtttgaa cttaccccat
     8401 cgcgatcgcg tagcatttac aatccatccc ataatcggat cgttaatagc atcccagatc
     8461 ctcgccacca gaaacaaagt accgaccaaa cccacagaca gcccgacgac atcggtgtag
     8521 taatacatga ggtacatata cacaatgccg atcgcaaaat ccttcccgaa cgctccaaat
     8581 ccataactga gttttgtagt cattgaaatg ctcatagggt atcgggtcgc tgttaccaac
     8641 gcccgccttc tgttattggt atcgtcagag gtgtgcgccg gaataaatcc cccggcgcag
     8701 tagcgtttag tcgcgtttta acggtgcaac cagcttggca gccagtcgcc gtgggcggca
     8761 atcaggtcgt caacaagagc atatatttcg tcaatgccca gcacggcggc agtatgcggg
     8821 tccatcatcg cggcgtggta aacacggtcg cgattttccg taagaatagc ttcggtcagc
     8881 agcgtctgta cgttgatgtt ggtttgcatc agggcggcca gatgcgaagg tagcgtaccg
     8941 actttggtcg gctgaatgcc attagcatca accagacagg ctacttccac gcaacatcct
     9001 tgtggcaggt tatcaatcaa accatcgtta cggacgttgc cgtaaatcac actcggctcg
     9061 ccagtccaga tagcgttcat gattgtgctg gcatattccc gtgacggttt aatatcaatc
     9121 cgggaggcgt ttttatactc ctccagctct ttatgccagt tcgccagctg ctcgacgcag
     9181 cgtttcgggt actcatccag cggtacttta taacgctcaa tcaaatcctc acgacctagc
     9241 ttaataaacc acggcgtata ttcggcaaaa tgttctgatg attccgtgac gaagtagccc
     9301 agctttttga acatttcgta gcgcacaata ttctggcagc gagtattacc atgaatattt
     9361 ggcttcggtg cctgccctgc ttcataagcc gccagcagtt ccgggtagag attcacgtaa
     9421 ctgccgtcgg cggttttgcg ctccagctcc aggtaaaacg ccatatggtt gatacctgcg
     9481 caacggtaac gcagcgtagc cgggtcgata ttgaggtcac gcgccagctc ttccgccgtt
     9541 ccctgcaccg aatggcacag cccgacctgt ttgatatacg gatagcgggc atacatcgcc
     9601 caggtattca tcgccattgg gttaacatag ttgagcatgg tggcatcggg gcagacttcc
     9661 gtcatgttct cgcaaatttg ccacagatgc ggaatggtac gtagcgcgcg cataataccg
     9721 cccggcccca acgtatcggc aatggtttgt tccagaccat gccgcttaca gacctcgaaa
     9781 tcagtcaccg tgcaaggttc ataaccgcca atctgaaatg ccaccacgac aaaatcggca
     9841 tcctctaagg cttctttctg ttgggtgtgg caggtgattt tgccgctggc ccctgctgaa
     9901 tccatcagct tacgcaccac aatatgcgac tcttccaggc gggtggggtc aatgtccatc
     9961 agggcaatat gcgccgtttt cagcgcctcg cgatggaaca catcaccaag aatattttta
    10021 acgaaaatcg tcgaaccagc gccgataaat gtaattttgg gtgcagacat catgcagatc
    10081 tcctggcttg cttgaataac ttcatcatgg caggcgaatc tgcagaaaac ctccggcttc
    10141 caggttgagc attcccataa actcagatcc tgcgtgaagc agcagtaaat ctgagtttat
    10201 gggaataaat tacctgaaaa gtagagtttt cggcctcatg atgctcggac agtcccctcg
    10261 cccctctggg aagagggtga aaaccgtgct cccactcgca gtcatcctcc ctcactcctg
    10321 ccataattct gatattctag gaaagagagc catccatgaa tacagatacg tttatgtgca
    10381 gcagcgacga aaaacagacc cgcagcccgc tgtcgctgta ttcggaatac cagcggatgg
    10441 agattgagtt tcgcgcaccg catatcatgc ccaccagcca ctggcatggt caggtcgaag
    10501 tgaatgtgcc tttcgatggc gatgtggaat acctgatcaa caatgaaaaa gtgaatatca
    10561 atcagggtca tatcacgctg ttctgggcct gtacaccgca ccaactaaca gataccggaa
    10621 cctgtcagag catggcgatt tttaatctgc cgatgcatct gtttctctcc tggccgctgg
    10681 ataaagacct gattaaccac gtcactcacg gcatggtgat caaatcactt gccacacagc
    10741 aacttagccc gtttgaagta cgccgctggc agcaggaatt aaacagtccg aatgagcaaa
    10801 ttcgccagct cgccattgat gaaattggcc tgatgctcaa gcgatttagc ctctctagct
    10861 gggaaccgat tctggtcaat aaaacctcgc gcacacacaa aaacagcgtc tcgcgccatg
    10921 cgcaatttta tgttagccag atgctgggct ttattgccga aaactatgat caggcgctga
    10981 ccatcaacga tgtggctgag cacgtcaaac ttaacgccaa ctatgcaatg gggatattcc
    11041 agcgggtcat gcaattgacg atgaaacagt acattaccgc gatgcgcatc aaccacgttc
    11101 gcgcgttact gagcgatacc gataaaagta ttctcgatat tgccctgacg gcaggctttc
    11161 gttcaagtag tcgtttttac agcacgttcg gcaaatatgt cggcatgtcg ccgcaacaat
    11221 accgcaaact tagccaacag cgccgccaga cgtttcccgg ctaaagacga tatcagtatc
    11281 agccaaaaaa atagtttagc cgtcgataat aagcagtgaa aataatttaa aataatcaca
    11341 taacgtattg tttataaaac taaatatttc aaaaatgttt gtttttcacg cgctttacag
    11401 cccgaaaagg ccggaagata cttgcccgca acgaagattc cttcataacc gggtaagcaa
    11461 tgatgaaagt attaattgtt gaaagcgagt ttctccatca agacacctgg gtcggtaacg
    11521 ccgttgagcg tctggcagat gctttaagcc agcaaaatgt taccgtgatt aaatccacct
    11581 cctttgatga tggttttgcc attctctctt caaacgaagc cattgactgc ctgatgttca
    11641 gctatcaaat ggagcatccg gacgaacatc aaaacgtcag acaattgatc ggtaagcttc
    11701 atgagcgcca acaaaacgtg ccggtcttcc tgttgggcga tcgggaaaaa gccctcgccg
    11761 caatggaccg ggatctgctg gagcttgtcg atgaattcgc ctggattctg gaagataccg
    11821 ccgactttat cgccggacgc gccgttgccg cgatgacccg ctaccgccag cagctgttgc
    11881 cgccactgtt cagcgcgctg atgaaatata gtgacatcca tgaatattcc tgggcagcgc
    11941 caggccacca gggcggcgtt ggttttacca aaacacccgc cggacgtttc taccatgact
    12001 actatggtga aaatctgttc cgtaccgaca tgggcatcga acgaacttcc ctcggttctt
    12061 tgcttgacca tactggcgca tttggcgaaa gcgaaaaata tgccgcacgc gtatttggtg
    12121 ccgatcgctc ctggtcggta gtcgtcggta cttccggctc taaccgcacc atcatgcagg
    12181 cttgcatgac cgataacgat gtcgtggtcg ttgaccgtaa ctgccataaa tccatcgaac
    12241 aaggtttgat gctgacaggc gcgaaaccgg tctatatggt gccaagccgc aaccgctacg
    12301 gcattatcgg gccaatctat ccgcaggaaa tgcaacctga aaccttgcaa aagaaaatca
    12361 gtgaaagccc gctgaccaaa gacaaagccg ggcaaaaacc gtcttactgc gtggtaacca
    12421 actgcaccta tgacggcgtg tgttataacg ctaaagaagc gcaggatctg ctggaaaaaa
    12481 cctccgatcg tctgcacttt gacgaagcct ggtacggcta tgcacgtttc aacccgatct
    12541 atgccgatca ctatgccatg cgcggcgaac ctggcgatca caacggtcct accgttttcg
    12601 ccacccactc catccacaaa ctgctgaatg cgctgtcaca ggcttcttat attcatgtac
    12661 gtgaaggtcg tggggcgatt aacttctccc gcttcaacca ggcctacatg atgcatgcca
    12721 ccacctcccc gctgtatgcc atctgcgcat ccaacgacgt ggcggtgtcg atgatggacg
    12781 gcaacagcgg cctgtcactg acacaggaag tgattgacga agcggttgat ttccgtcagg
    12841 cgatggcgcg gctatataaa gagttcaccg ctgacggtag ctggttcttc aaaccgtgga
    12901 acaaagaagt cgtcaccgac ccacaaaccg gcaaaaccta tgactttgct gacgcaccaa
    12961 ccaaactgct gaccaccgtt caggactgct gggtaatgca tccgggcgaa agctggcacg
    13021 gcttcaaaga tattccggat aactggagta tgctcgaccc aattaaagtc agcatccttg
    13081 ctccgggaat gggtgaagat ggtgaactgg aagaaaccgg tgttccggcg gcgctggtca
    13141 ctgcctggct tggtcgccac ggcattgtgc ctacccgcac cactgacttc caaattatgt
    13201 tcctgttctc tatgggcgta acccgtggga aatgggggac tctggttaac accctttgct
    13261 ccttcaaaca ccactatgac gccaacacac cgctggcgca ggtgatgccg gaacttgttg
    13321 aacaatatcc tgacacttac gcgaacatgg ggattcacga tctgggtgac accatgtttg
    13381 cctggctgaa agaaaacaac cctggcgcac ggttgaacga agcctattcc ggcctgccgg
    13441 tggcggaaat caccccgcgt gaagcgtaca acgcgattgt cgacaacaat gtcgaactgg
    13501 tatccattga aaatctgcca ggacgcatcg cggcaaactc agttatcccg tatccgccag
    13561 gaatcccgat gctgctgtct ggtgaaaact tcggcgataa aaacagtccg caagtaagtt
    13621 atttacgctc gctgcaatcc tgggaccacc atttccctag atttgaacac gaaactgaag
    13681 ggactgaaat tattgacggt atttaccacg ttatgtgcgt gaaagcgtaa ccactattcc
    13741 gctgaaggcg taattgttta aataacatta cgccgcctgg ccttaggcct tttgagtatg
    13801 gcaacgtttt cataaaaatt gctgcaaaca aaaatgtcat actttttgcg cggccccacc
    13861 ccacgctttt ttttgcctgt tatttatcct gtaaaaaaat atgtacatga gaaattacta
    13921 taaaaatttg taatattagt aaaactcgtt atttttatgc atgtttatat tcatcatgca
    13981 attatataac catttcccgg tatcgctttg ctttagcgag aactggtctt ttattcgcac
    14041 tcaggagtac atgtatgagg atttgcagcg accaaccttg tattgtttta ttgactgaaa
    14101 aagatgtctg gataagggtg aatgggaaag aacctattag ccttaaagct aaccatatgg
    14161 cgttattaaa ttgtgaaaat aatattatcg acgtctcctc tcttaacaac actttggttg
    14221 ctcatattag tcacgacatc atcaaagatt acctccggtt tctgaataaa gatctctcgc
    14281 aaataccagt atggcaacgt agcgctacgc ccatactcac cctgccatgc ctgacgccag
    14341 acgtctttcg cgttgccgcg caacacagca tgatgcccgc agaaactgag tcagaaaagg
    14401 aacgaacacg cgcattatta ttcacggtgc tatcccgttt tctcgacagt aaaaaattcc
    14461 tttcattaat gatgtatatg ttacgtaatt gtgtaagtga cagcgtttat caaattattg
    14521 aaagcgatat tcacaaagac tggaatctta gtatggtagc cagttgttta tgtcttagcc
    14581 caagtctgtt aaagaaaaag ttgaaaagcg aaaacaccag ttatagccaa ataatcacca
    14641 cctgccgcat gcgttatgcc gtaaatgaat taatgatgga cggtaaaaat atctctcagg
    14701 tatcacagtc ctgcggctac aacagtacgt cgtactttat ttctgtcttt aaagacttct
    14761 acggtatgac gccgctgcat tatgttagtc agcacagaga acgcactgtc gcctgatttt
    14821 taaccttaac gaagagctat attaataacg gcatcagcga taacccggtc gataataatt
    14881 caactatcga atgcaggcgt atgatatgac gtaatttatt gtcacgaagc tcgccttcgc
    14941 aggagtttaa ttatgtcttc ggatgctgat gctcacaaag tgggcttaat ccccgtcacc
    15001 ctgatggtgt cggggaatat tatggggtca ggtgtttttc tgttacctgc aaacctggcc
    15061 tccactggcg ggattgccat ttatggatgg ttggtgacga ttatcggtgc gctggggctc
    15121 tcgatggtat acgccaaaat gtcgttcctc gacccaagtc ctggtggttc ttacgcttac
    15181 gcccgccgct gctttggccc gtttctcggt tatcaaacca acgtcctcta ctggctggcc
    15241 tgctggatcg gcaatatcgc catggtggtc attggcgtag gatatttaag ttacttcttc
    15301 ccgattctga aagatccatt ggtattaacc atcacctgcg tcgtggtgct gtggatcttc
    15361 gtcctgctga acattgtcgg tccgaaaatg atcacccgcg tgcaggccgt tgccactgtg
    15421 ctggcgctga tccccatcgt cgggattgcc gtatttggct ggttctggtt ccgtggtgaa
    15481 acctatatgg cggcatggaa cgtcagcggc ctgggcacct tcggtgcgat tcaaagtacc
    15541 cttaacgtta cgctgtggtc gttcatcggt gtggaaagtg cctccgttgc cgcaggtgtg
    15601 gtgaaaaacc cgaaacgcaa tgtccctatc gccaccattg gtggggtatt gattgccgcc
    15661 gtttgctatg tgctctccac caccgcgatt atggggatga tccctaatgc cgcactgcgc
    15721 gtttctgcct cgccattcgg tgatgccgca cggatggcgt tgggtgacac cgctggggcc
    15781 attgtttcct tctgcgcagc tgcgggttgc ttaggttcac tgggcggctg gacgttgctg
    15841 gcgggtcaaa cggcgaaagc cgctgccgat gacggactgt tcccaccgat ttttgcccgt
    15901 gtaaataaag cgggtacgcc agtggcgggg ctgattatcg tcggtatttt gatgaccatc
    15961 ttccagctca gcagcatttc accaaacgcg accaaagagt tcggtctggt ttcttccgtc
    16021 tcggtcatct ttacactggt gccatatctt tacacctgtg cggcgttact gctgctcggc
    16081 cacggtcact ttggtaaagc acgcccggca tatctggctg ttactaccat tgccttcctc
    16141 tactgcattt gggccgtggt ggggtccggg gcgaaagagg ttatgtggtc atttgtcacc
    16201 ctgatggtca tcaccgccat gtatgcgctg aattacaacc ggctacataa aaacccgtat
    16261 cccttagatg caccaataag caaagattaa ttccccttaa tccagcaaac ataaaagcca
    16321 accttaagaa cttaaggttg gcttaatttt gctttgcgag catatgcgca ctttgttcga
    16381 tggaaacacc gtgatgttga agcgcctact aaaaagaccc tctttgaatt tactcgcctg
    16441 gctattgttg gccgcttttt atatctctat ctgcctgaat attgcctttt ttaaacaggt
    16501 gttgcaggcg ctgccgctgg attcgctgca taacgtactg gttttcttgt cgatgccggt
    16561 tgtcgctttc agcgtgatta atattgtcct gacactaagc tctttcttat ggcttaatcg
    16621 accactggcc tgcctgttta ttctgattgg cgcggctgca caatatttca taatgactta
    16681 cggcatcgtc atcgaccgct cgatgattgc caatattatt gataccactc cagcagaaag
    16741 ttatgcgctg atgacaccgc aaatgttatt aacgctggga ttcagcggcg tgcttgctga
    16801 gctgattgcc tgctggctaa aaatcaaacc tgccacctcg cgtctgcgca gtgttctttt
    16861 ccgtggagcc aatattctgg tttctgtact gctgattttg ctggtcgccg cactgtttta
    16921 taaagactac gcctcgttgt tccgcaataa caaagagctg gtgaaatcct taagcccctc
    16981 taacagcatt gttgccagct ggtcatggta ctcccatcag cgactggcaa atctgccgct
    17041 ggtgcgaatt ggtgaagacg cgcaccgcaa cccgttaatg cagaacgaaa aacgtaaaaa
    17101 tttgaccatc ctgattgtcg gcgaaacctc gcgggcggag aacttctccc tcaacggcta
    17161 cccgcgtgaa actaacccgc ggctggcgaa agataacgtg gtctatttcc ctaataccgc
    17221 atcttgcggc acggcaacgg cagtttcagt accgtgcatg ttctcggata tgccgcgtga
    17281 gcactacaaa gaagagctgg cacagcacca ggaaggcgtg ctggatatca ttcagcgagc
    17341 gggcatcaac gtgctgtgga atgacaacga tggcggctgt aaaggtgcct gcgaccgcgt
    17401 gcctcaccag aacgtcaccg cgctgaatct gcctgatcag tgcatcaacg gcgaatgcta
    17461 tgacgaagtg ctgttccacg ggctggaaga gtacatcaat aacctgcagg gtgatggcgt
    17521 gattgtctta cacaccatcg gcagccacgg tccgacctat tacaaccgct atccgccgca
    17581 gttcaggaaa tttaccccaa cctgcgacac caatgagatc cagacctgta ccaaagagca
    17641 actggtgaac acttacgaca acacgctggt ttacgtcgac tatattgttg ataaagcgat
    17701 taatctgctt aaagaacatc aggataaatt taccaccagc ctggtttatc tttctgacca
    17761 cggtgaatcg ttaggtgaaa atggcatcta tctgcacggt ctgccttatg ccatcgcccc
    17821 ggatagccaa aaacaggtgc caatgctgct gtggctgtcg gaggattatc aaaagcggta
    17881 tcaggttgac cagaactgcc tgcaaaaaca ggcgcaaacg caacactatt cacaagacaa
    17941 tttattctcc acgctattgg gattaactgg cgttgagacg aagtattacc aggccgcgga
    18001 tgatattctg caaacttgca ggagagtgag taaatgaaaa ttctgattgt tgaagacgat
    18061 acgctgttat tgcagggact gattctggcg gcgcaaaccg aaggctacgc gtgcgatggc
    18121 gtgacaaccg cgcggatggc ggaacaaagc cttgaggacg gtcattacag cctggtggta
    18181 ctggatttag ggttacccga cgaagatgga ctgcattttc tcgcccgtat ccggcagaaa
    18241 aaatacaccc tgccggtact gatcctcacc gctcgcgata cgctgaccga caaaatcgcc
    18301 gggctggatg tcggtgccga tgactatctg gtgaagcctt ttgcgctgga agagttacac
    18361 gcccgtatcc gcgccctgct acgacgccat aataatcagg gcgaaagtga gctgattgtt
    18421 ggcaatctga cgctgaatat gggtcgccgt caggtatgga tgggcggtga agagttgatt
    18481 ctgacgccca aagaatatgc tctgctgtca cggttaatgc tcaaagcagg cagtccggta
    18541 catcgggaaa ttctctacaa cgacatctat aactgggaca atgaaccctc gaccaacacc
    18601 ctggaagtgc atatccacaa tctgcgcgac aaagtgggca aagcccgtat ccgcaccgtg
    18661 cgcggctttg gctatatgct ggtcgcgaat gaggaaaact aattgaatct gatgcgtttt
    18721 ctgcgccgac ctatatcgct gcgccaacgg ctgatattga ccatcggggc cattttgttg
    18781 gtgtttgagc tgatcagcgt cttctggcta tggcatgaaa gtaccgagca gattcagctg
    18841 tttgagcagg cgctacgcga caatcgcaac aacgatcgcc acatcatgcg tgagatccgc
    18901 gaggcggtcg ccagcctgat tgtccccggc gtctttatgg tcagcctgac gctatttatc
    18961 tgctatcagg cggtacgccg catcacccgc ccgctggcgg agctgcaaaa agagctggaa
    19021 gcgcgcaccg ccgacaactt aacgcccatt gccattcaca gcgccaccct cgaaatcgaa
    19081 gcggtggttt cggcgttgaa cgatctggtc agtcgcctga ccagcacgct ggataacgaa
    19141 aggttgttta ccgctgacgt cgcgcacgaa ctgcgaacgc cactggcggg ggtgcgtttg
    19201 catctggaac tgctggcgaa aacgcatcac attgatgtag caccgttagt ggcacggctt
    19261 gatcagatga tggagagcgt ctcccagctg ctgcaactgg cgcgtgccgg acagtcattt
    19321 tcttccggta attatcaaca tgtaaaactg ctggaagatg tcattctccc ctcgtatgac
    19381 gaactcagta ccatgctcga ccagcgacag caaaccctgc tattgccaga gagcgccgcc
    19441 gacatcaccg ttcagggtga tgcaaccctg ctgcggatgt tattgcgaaa cctggtagaa
    19501 aacgcccatc gttacagccc gcaaggcagc aacattatga ttaagctgca agaagatggc
    19561 ggagcggtca tggcagttga agatgaagga ccaggtattg atgagagtaa atgcggggag
    19621 ttgagtaaag cgtttgtacg tatggacagc cgttatggcg ggattggtct ggggttaagt
    19681 attgtcagcc gcattacaca gttgcatcac gggcagtttt tcctgcaaaa ccggcaagag
    19741 acttccggca cgcgggcctg ggtacggctg aagaaagatc agaacgtggc aaaccagata
    19801 taaagaaagc tgctgaccac cagcacgctg aacacggtag ttaaactttc ataaacacgg
    19861 tttttcattg cactctcctc cgttaacctg gaggagagta tgcgcgtcag agattaagtg
    19921 aaccttaaga gttcaattgg cctgataaga cagcgtcaca tcaggccatc cgtttcagct
    19981 tattcatcaa ttcgcggatg ttgctgcacc aggcgggtac gtttcgcctg caaatcggca
    20041 atctcgtggt caatattatc gattttctgc tcgatattat cgtaatgctc gacgagaatt
    20101 tccttcgctt cctgtatatc tgacgccgcc ggtgtcgcac ctttcaacgg acgatttgcc
    20161 gtctctttca tggttacgcc ggtgattaaa ccaatcaccg ccactaccat caggtaatag
    20221 gcaggcatca tcagattctg cgagctttcg accagccagg ccgccagtgt tggcgtcaga
    20281 ccggcaacca gcaccgaaat attaaatgcc gccgccagcg cgctgtaacg gatatgcgtc
    20341 gggaacatcg ctggcaaggt agaagccata acgcccataa agcagttaag gatcaccgcc
    20401 agcatcagta acccggcaaa aatcaggccg atgacgttac tgttaatcag aataaacgcc
    20461 gggatcgcca acacaaacag tgcaacacta ccaagtagca caaacggacg acggccaaaa
    20521 cggtcactca gcaagcccat caccggctgg acaaacagca taccgatcat aatggcgata
    20581 ataatcagca ccccgtggtc ttcggagtaa tgcaggttat gcgacaaata actcggcata
    20641 taggtcagca acatgtagta agtcacgttg gtggcaatta ccagaccaat acatgtcaac
    20701 aggctgcgcc agtatttagt ggcaatctct ttaaacgaga ctttcgggcc atcctgcaaa
    20761 ccttcgcggt cgccctgttc cagtttatca acatgctgct ggaacgccgg agtttcttcc
    20821 agcgcatggc gcaggtaaag cccgataatc cctaacggca gagcaataaa gaacggaata
    20881 cgccagcccc agtcgaggaa gttcgcttcg ccgacaatgg tcgaaattaa caccaccacg
    20941 cccgcaccca gcacaaaccc ggcaatagaa ccgaagtcca gccagctgcc cataaagcca
    21001 cgtttacggt caggggagta ttccgcaaca aatatcgacg ccccggtata ttcaccaccg
    21061 accgagaaac cttgtgccat cttacagatc aacagcagaa tcggtgccca aataccaatc
    21121 gtgtcgtagg acggtattaa gccaatacag aacgtactga tcgacataat cacaatagtg
    21181 atagcgagga tcttctggcg accatattta tcgcccaaca taccaaagaa gagtccgcca
    21241 agcggtcgaa tcagaaaggg aacggagaaa gtggcaagtg cagcaaccat ctgcacgctg
    21301 gggtcagccc ccgggaaaaa aactttacct aatgcgtaag caacaaaacc ataaacacca
    21361 aaatcaaacc attccattgc attacccagt gatgctgcgg taatggcttt acgcagttta
    21421 ccgtcatcaa taatggtgac atcacgaagg gtaatcggtt ttactttttt ccttttcagc
    21481 atagctttcc tcgcagagtt ggcccatttc cgcaaacgcc agaaaaatca gcgcgacggc
    21541 cgcaaaaggg gagcgggaat tgcaggatgc aatctctgta acaagcgtag cgggtttgct
    21601 tacaccctcc ggttaaggaa tgtacaatca aaccagttga cgcctgaatt gggcagttaa
    21661 tgaccttttt accctaccag tgtttaaatt tgtgatcaac ttcacacatg gatttcgttt
    21721 aggactcatt gatgtaactg attatttacg ccaaatttac agagaagatt tcctcgacca
    21781 ttgcccccac caaacgctgc gctgaacaga gtcgtggcat ccccagcgcg acagtttgcc
    21841 agcgttgggt cactgaccag caaacgggtt gtttatctgc cgtggtccac tttcccagcc
    21901 aacttaaaaa atcagcatga tccccgcccg tcggaacggt cgaagaggca aaccactgat
    21961 ggtaatagtg acgcgtagtc ggtgccgcat tgatactctc tgccagatgt tgtgtcgctt
    22021 tctcgcgcaa atcacccagc aacaacacca ccgcttgcgg gcttcccaga cggcaggcat
    22081 cgccaaatgc accccagcgc aactcctcca gaacgataaa acagcgccca ggcaacgacc
    22141 aggcgttata cgccccagca cgccagcggg taaaaatttg ctcgctctga tcacctgcct
    22201 gcaccagtag gcctcgttgc gttaacgcct tttccttcca gcgacaacgg gctgaaaacc
    22261 actcatacaa ggcggcaatt tgctggggga gctccgggaa agcagagtgc tggcggtgat
    22321 ccattttttg cagcagcgcc agatgctgcg cgatctgcgt cagcgccaga tgaccgggat
    22381 ccagcatcga cgccagtttt tccaccattg tcagtcgcag gctaagcggc gcatttaaca
    22441 tgccgccgtt cgtccatgtc tgaagctgca cacgcgaaag aagttcctgc atcagtcgtt
    22501 cacgaaactg ctgctgatgg gcttgaggat ggcgggcatc atcgccctgc gccagatcca
    22561 ctaaaaagcg cggatgaacc gactccagca cgcgaccggg gccgtccagt aacgtcttgg
    22621 tcaatatcgt tctgccgtga aaagtgtttg aatatcatcg cgtaacagct gagcgtcggt
    22681 gtaaatccag ctgtgagtca tcacagtctg ctgcaattgc tgctgcatca gcctgaccac
    22741 cgattcattt tgttgacgca gagccagact ttcgcgtaaa cgcgtctgta attctgtcaa
    22801 acatgaagcg aactcagcga aaaaagtatt catgcctgcc gtaacagatt catcgacctg
    22861 ctctgccaga actttagcca tttgttggca atagagatcg acttctgcgc ttaatgctcg
    22921 ttgcaacaca ctgtaatcaa ccgtttctgt cggggatttc tcatttcccc gtccccagtc
    22981 gggctgattc aaccagcgcg aaaaagtctc acgcacaacg cctaaacgcg tgctctgctc
    23041 gtccgttgct tcctggcgcg aaatgacttc actgaacagc tggcgagtgt tgaagtgggg
    23101 aactacgccg tgaaaaacag gaaaatgaaa cccaggacga aaccctgact cgctcaattc
    23161 cattttgact tgttgctcaa tggggcgaat aacatcggtt aacacccggc aaagggtgga
    23221 ttccatctcg gcaaaacgca gcgtaaagtc gcgactgatg gtgttctgcg ccgtctgtaa
    23281 cagtgtctca cagcgggtac gaatctcgct taacggctcc gaatcatcct gaaacaaggc
    23341 ggctaactgc gcattcaccg catcttgctg ttgacgcaga aagtggttgg cggaggtcag
    23401 ggccagctcg atttcatgtt taatctcgcc gctcacctgc gcctgattga gttgcaatag
    23461 ctgcaaactt tcttcgacct gatggatatt ttgccgcaat tgttcacaag cgacgtttaa
    23521 cccgtgcgca cgaaaatcca ggtattcccg cgcctgctgc gcgtaattca acagtttatg
    23581 cgcagcagat cgcagagcat acaatgaggc gttagcgtaa gcggcatgaa gcaacgcctg
    23641 aattggctgg gcgaacagcg aatcttccca caactgatcg gcagcatgac gaatatgttc
    23701 gaggtccgcc agatcggcat gacgccagcg cctgccgagc gcggcatggg caaaatcttc
    23761 cacccagcgt tgttgctctg gcgctggtaa cttaccgttg ttggctaact catagcgcgc
    23821 ccgattcgcc aggtagcccc acatcgacga caccgggaat atctgctgtg gcgtaataca
    23881 gcctttcatc agcgtcccgg aaatcagtgc ccgcacctgg tcggcgtcgt cactgttacg
    23941 atcctgttga tcgaacttat tgaccagcac atacagcggc accgattgcc ccaccgccaa
    24001 aatcgcctca cggacctctt catcggagat cgatttcagt tgcgtataat ccagcaccgc
    24061 cagtaccgcc gaggcgcgtg ccagctgctg gttaagcatt ttttgcagat gcggttgccc
    24121 ggcttcattt ggcccggggg tatccagtaa cgtcaactga ccgggataac tttccagccc
    24181 cgccagatgg acaaactcca cttcaatcac gggaatatgc tcaatggcgg cgtaagcaga
    24241 aaaaggaaaa tcgacgtcca gcgccttcgc cagtcgcact aaatcattca aacttttcag
    24301 acaatgaaaa ataggctggg cacccagata atatttttcg aaagcgacgc cattttcgat
    24361 ccgctgcata agcgcacgca tatctttatc tatttccagc acatcggtca gatgcttaat
    24421 atcgcaatca cgcaggcgct gttgtaattg ttgaattaaa caatcgattg gcgcgacatg
    24481 tgaaaaatgc agtaccggtt ccttttgccc gggcgtatgg cgaataagcg tcggcagcgc
    24541 agtcattggg cgattacgat taggcagaac ctccgtacca acaatggcat taatggtggt
    24601 tgatttccct gctttcatgg taccgacaat tgccagcacc atttccagtc gggaaatttt
    24661 acacaactca ttattcagca tcgcgtgacg ttcggcgata ttaggctgac tccagggtaa
    24721 agccagttgt ggcgcgtcgt ctccgggtac agagagaggc attttttcca gtaactgcaa
    24781 ctgttggcga gaaagctgta acaggcgttc agcctcctga cttaactcat acagggtctg
    24841 tgtgtacata gaaaattctt ccttaaagca aattttgtta cttttattta gccagattgt
    24901 ttttgagttc tgttttcggc ttttataatt actgcaagaa ataattttat atttagtgtg
    24961 ttgttttttt atcagaataa ataacgtctt ctgatgcgtt taaaacgtca gaaagataaa
    25021 aatatcatgt gaataaaaaa aagaacaagt agagcattaa cattatctta aataataaat
    25081 agaggcaaaa agattatttt ctttttgcgt ttcctttcaa atgaaaacga tcgtcgtcta
    25141 aaatcagcag tacccccgac aaactcaggg attttgtgta taattgcggc ctttttcggc
    25201 aatctgccgt tttttggcgc ttttgccctg ctgacttttg aggaaatcca catgtcatta
    25261 ccacactgcc caaaatgcaa ctccgaatac acttacgaag ataacggcat gtacatctgc
    25321 ccggaatgtg cctacgaatg gaacgacgca gaacctgcac aggaaagcga cgagctgatc
    25381 gttaaagatg ctaacggcaa tctgctggct gacggcgaca gcgttaccat cattaaagat
    25441 ctgaaggtga aaggtagctc ttcgatgctg aaaattggca ccaaagtgaa aaacatccgc
    25501 ctggttgaag gcgaccataa catcgattgc aaaatcgacg gttttggtcc gatgaaactg
    25561 aaatctgagt ttgtgaaaaa gaactgattg tattgtgatc ggtaagccgg ataaggcgct
    25621 cgcgccgcat ccggcaacga tgccgactgc ctgatgcgac gcttgcgcgt cttatcaggc
    25681 agcctctcat ctgtataaat ttcgaactac acttaactgg cttctcttaa ctgaggtcac
    25741 catcatgccg ttaagtccct acctctcttt tgccggtaac tgttccgacg cgattgccta
    25801 ttatcaacgt acgttgggcg cggaactgct ctataaaatc agcttcggcg aaatgccgaa
    25861 atcagcgcag gacagcgccg agaactgccc ttccggaatg caatttcccg ataccgccat
    25921 cgctcatgcc aacgtgcgca ttgccggaag cgacatcatg atgagcgatg ctataccgtc
    25981 aggaaaagcc agctactccg gctttacgct ggtgctcgat tcgcaacagg tcgaagaagg
    26041 aaaacgctgg tttgataacc ttgccgctaa cggaaaaatc gaaatggcct ggcaggaaac
    26101 tttctgggcg catggctttg gcaaagtcac cgataaattt ggcgtaccgt ggatgattaa
    26161 tgtcgtcaaa caacaaccaa cgcaataacc cgccgggagg cccgccctcc cgcactgtca
    26221 tcgaattccc gttaactctt catctgttag tcacttttaa ttaaccaaat cgtcacaata
    26281 atccgccacg atggagccac ttttttaggg aggctgcatc atgcaaacga ttatccgtgt
    26341 cgagaagctc gccaaaacct tcaatcagca tcaagcgctg catgcggttg atctgaacat
    26401 tcatcacagt gaaatggtgg ctctgcttgg gccgtcgggt tccggcaaat ccaccctttt
    26461 acgtcattta agcggtttga ttaccggcga taaatccgcc ggtagccata tcgagctact
    26521 gggccgcact gtccagcgcg aaggccgcct ggcccgcgat atccgcaaaa gccgcgccaa
    26581 caccggctac atctttcaac aattcaacct ggtgaaccgc ctgagcgtac tggagaacgt
    26641 gctgattggc gcgctcggca gcacgccgtt ctggcgcacc tgttttagct ggttcaccgg
    26701 cgagcagaaa cagcgcgcgt tacaggcgct gacccgcgtt ggcatggtgc attttgccca
    26761 tcagcgcgtt tccaccctct ccggcggcca gcagcaacgt gtggcgattg cccgtgcgct
    26821 gatgcagcag gcgaaagtga ttctggccga tgaacccatc gcctcgctgg acccggaatc
    26881 agcgcgcatc gtgatggaca ccctgcgcga catcaaccag aacgacggca tcaccgtggt
    26941 cgtcacgctg catcaggtgg attacgccct gcgctactgc gaacgcatcg tcgccctgcg
    27001 tcaggggcac gtcttctacg acggcagcag ccaactgttt gataacgaac gttttgacca
    27061 tctctaccgc agcattaacc gcatcgaaga gaacgcgaaa gctgcctgat atccccatca
    27121 ttgaggaaaa cgaatgaacg ctaagataat tgcctcgctg gccttcacca gcatgttcag
    27181 cctcagcacc ctgttaaccc cggcacacgc cgaagagcag gaaaaggcgc tgaatttcgg
    27241 cattatttca acggaatcac agcaaaacct gaaaccgcaa tggacgccgt ttttacagga
    27301 tatggagaag aaactgggcg tgaaggtcaa cgccttcttt gccccggact acgcgggcat
    27361 tatccagggg atgcgcttca ataaagtgga tattgcctgg tacggcaacc tgtcggcgat
    27421 ggaagcggtg gatcgcgcca atggccaggt cttcgcccag acggtcgcgg cggatggatc
    27481 gccgggttac tggagcgtgt tgatcgtcaa caaagacagc ccgatcaaca acctgaacga
    27541 tctgctggcg aagcggaaag atctcacctt cggcaatggc gatcctaact ccacctctgg
    27601 cttcctcgtc cccggttact acgtcttcgc taaaaacaat atctccgcca gcgacttcaa
    27661 gcgcaccgtc aacgccgggc atgaaaccaa cgcgctggcc gttgccaaca agcaagtgga
    27721 tgttgccacc aacaacaccg aaaacctcga caagctgaaa acctccgcgc cggagaagct
    27781 taaagagctg aaggtgatct ggaaatcgcc gctgatccta ggcgatccga tcgtctggcg
    27841 caagaatctg tccgaaacta ccaaagacaa gatctacgac ttctttatga actacggaaa
    27901 aacgccggaa gagaaagcgg tgctggaacg cctgggctgg gcgccgttcc gcgcttccag
    27961 cgacctgcaa ctggtgccga ttcgccagct cgcactgttt aaagagatac aaagcgtgaa
    28021 aggcaataaa gggctgaatg agcaggacaa gctggcgaaa accaccgaga ttcaggcgca
    28081 gctggatgac ctggaccgcc tgaacaacgc gttaagcgcg atgagttcgg tgagtaaagc
    28141 ggtgcagtaa atcgtaggtc ggataagacg ccccggcgtc gcatccgaca atgtgtaggc
    28201 gttgatgccg gatgcggtgc aagcacctta tccggcctac agaccggagc caaacatgca
    28261 aaccatcacc atcgccccac ccaagcgtag ctggttctcg cttctgagct gggccgttgt
    28321 tctcgacgtg ctggtcgtct cgtggcaggg cgcggaaatg gccccgctta cgctgattaa
    28381 agacggcggc aacatggcaa cc
//
LOCUS       DAJTSA010000050        28364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000050 DAJTSA010000000
VERSION     DAJTSA010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.050"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="OO369_002273"
                     /pseudo
     CDS             complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="OO369_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(1023..2045)
                     /locus_tag="OO369_002274"
     CDS             complement(1023..2045)
                     /locus_tag="OO369_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5982532.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2059..3561)
                     /locus_tag="OO369_002275"
     CDS             complement(2059..3561)
                     /locus_tag="OO369_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5982533.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="OO369_002276"
                     /pseudo
     CDS             complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="OO369_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4967..5497
                     /gene="ppa"
                     /locus_tag="OO369_002277"
     CDS             4967..5497
                     /gene="ppa"
                     /locus_tag="OO369_002277"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR5982534.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5877..6218)
                     /locus_tag="OO369_002278"
     CDS             complement(5877..6218)
                     /locus_tag="OO369_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR5982535.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="OO369_002279"
     CDS             complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="OO369_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR5982536.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="OO369_002280"
     CDS             complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="OO369_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR5982537.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11936..12574
                     /gene="msrA"
                     /locus_tag="OO369_002281"
     CDS             11936..12574
                     /gene="msrA"
                     /locus_tag="OO369_002281"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR5982538.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12897..14240
                     /locus_tag="OO369_002282"
     CDS             12897..14240
                     /locus_tag="OO369_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5982539.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14302..14508)
                     /locus_tag="OO369_002283"
     CDS             complement(14302..14508)
                     /locus_tag="OO369_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR5982540.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14833..15387
                     /locus_tag="OO369_002284"
     CDS             14833..15387
                     /locus_tag="OO369_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5982541.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15450..16388)
                     /locus_tag="OO369_002285"
     CDS             complement(15450..16388)
                     /locus_tag="OO369_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5982542.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16600..17340)
                     /gene="cysQ"
                     /locus_tag="OO369_002286"
     CDS             complement(16600..17340)
                     /gene="cysQ"
                     /locus_tag="OO369_002286"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR5982543.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17530..19473
                     /gene="cpdB"
                     /locus_tag="OO369_002287"
     CDS             17530..19473
                     /gene="cpdB"
                     /locus_tag="OO369_002287"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR5982544.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19599..19979)
                     /locus_tag="OO369_002288"
     CDS             complement(19599..19979)
                     /locus_tag="OO369_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5982545.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20068..20928
                     /gene="qorB"
                     /locus_tag="OO369_002289"
     CDS             20068..20928
                     /gene="qorB"
                     /locus_tag="OO369_002289"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5982546.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21037..22002
                     /locus_tag="OO369_002290"
     CDS             21037..22002
                     /locus_tag="OO369_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5982547.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22110..22772
                     /gene="ytfE"
                     /locus_tag="OO369_002291"
     CDS             22110..22772
                     /gene="ytfE"
                     /locus_tag="OO369_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR5982548.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22817..24229)
                     /gene="cycA"
                     /locus_tag="OO369_002292"
                     /gene_synonym="dagA"
     CDS             complement(22817..24229)
                     /gene="cycA"
                     /locus_tag="OO369_002292"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR5982549.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24537..25157)
                     /gene="fklB"
                     /locus_tag="OO369_002293"
     CDS             complement(24537..25157)
                     /gene="fklB"
                     /locus_tag="OO369_002293"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5982550.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25376..25921
                     /locus_tag="OO369_002294"
     CDS             25376..25921
                     /locus_tag="OO369_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HCR5982551.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25905..26552)
                     /locus_tag="OO369_002295"
     CDS             complement(25905..26552)
                     /locus_tag="OO369_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5982552.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26764..27555
                     /locus_tag="OO369_002296"
     CDS             26764..27555
                     /locus_tag="OO369_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCR5982553.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27565..>28364
                     /locus_tag="OO369_002297"
     CDS             27565..>28364
                     /locus_tag="OO369_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR5982554.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDIN"
BASE COUNT         6961 a         7551 c         6991 g         6861 t
ORIGIN      
        1 tccttaactg atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga
       61 ctcttgtcac tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg
      121 ctataaaaat aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc
      181 cgtcaaagtt gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa
      241 gcaccgtccc gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt
      301 ccagttccac acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa
      361 tccccgccag tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag
      421 tagaaatccc catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc
      481 caaaacgggt acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc
      541 ccatcgcact aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat
      601 aaatgggatg gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa
      661 agaacatccc cgcaagcgta atgataaatg ccgggatctt cagggcgtcg atcagaagcc
      721 ccataaatgc gccgaaggca cagcccatca ccagcactag cggaaacgcc agcagcggcg
      781 agaggccgaa atcgccaacc acttttgcca gaaacacgcc agtaaaggcg atcaccgaac
      841 cgacggagag atcgataccg ccagagagga tcacaaaggt catgccaacg gcgattatcc
      901 caagaaaggc gttatcggtc aggatattgc agatcaccct tgtggaagca aaaccgggaa
      961 actgggtcag gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagcggca
     1021 agttacgttt tatcatggct acgtactcct ttaatcagac tgataaagcg ttgcgactga
     1081 acaatcagca cacaaagcac caccaccgct ttcaccacct ggttcatctc cggcggaaag
     1141 cccgaaagca aaattccggt gttcatcccc tgaataatca gcgcccccac taccgagagc
     1201 agcaggttaa agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg
     1261 tccagctcca gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg
     1321 ataatgcccg caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt
     1381 cgcgtgttta ccccggcatt ttttgccgcc cgaatgttga taccaacgac ttcgataaac
     1441 atccccagcg ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata
     1501 atgaccggcg ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag
     1561 ttaaacgtga cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc
     1621 aggatcaggg tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac
     1681 aatcccgcca ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc
     1741 gcgaccgtca tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga
     1801 tcgataccac cggtggcgat caccagcgta ataccaatcg ccagtaacgc aacgggagct
     1861 gcacggttaa gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc
     1921 cagaaatgcg gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc
     1981 ggcatccccg tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga
     2041 ggcatcacac tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg
     2101 tgccagcggg atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata
     2161 gcccaccagc tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag
     2221 cgtttcaatc aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttagctc
     2281 atcaaggatc agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg
     2341 attgccaccg gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg
     2401 gcggataaag cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc
     2461 gcgctgggcc tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc
     2521 atcggttttc ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga
     2581 tcgcagggtt tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc
     2641 gaagatcact tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat
     2701 ctcgccgggg cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt
     2761 gaacgcggca acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt
     2821 atccagctcg cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc
     2881 ccgacagcct acgaaactgc cgttgcgtaa gacggtgatc cgatcgctga cctgatagac
     2941 ctgatcgaga aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat
     3001 caggccaaat aatagctcca tttcctgggt gtcgaggctg gcggtgggtt catcgaggat
     3061 cagcactttg gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgccac
     3121 cgaaaaacgg ttaagcggtt cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc
     3181 ggtggcgcgc ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc
     3241 tatgaataga ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt
     3301 gccgataccg agttgttgtg catgggcggt atttttcggt gagatagtct ggccttccag
     3361 ccagatggtg ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt
     3421 tcccgccccg ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac
     3481 gttgtctaac gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc
     3541 ctggtgttgg tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga
     3601 taagacgcgg taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg
     3661 ccttatccgg cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct
     3721 tcttctctaa ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca
     3781 gcttttcagg catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg
     3841 ccatgttcgg cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga
     3901 tgtccggtac accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt
     3961 ctttaattgc ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga
     4021 tgtttttgcc gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc
     4081 gggtgaagtc acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt
     4141 cggcaaagcc tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca
     4201 ccacgttgca tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt
     4261 cgaggatgtt gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg
     4321 aacggtcgag caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc
     4381 aacccgtcgc aaccactgga gcgataaaga tcgcatccac cccttatgca acgaaggaac
     4441 gtaccgcttt aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc
     4501 cgcgtttttc ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg
     4561 atccgacctg cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg
     4621 ccgagactgc agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat
     4681 tattattggt gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg
     4741 cgttacatca cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcactttc
     4801 atctcactat gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa
     4861 aactggcgaa aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct
     4921 ataatacttg gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa
     4981 cgtccctgcg ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa
     5041 cgcagatccg atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt
     5101 catgtccacc gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct
     5161 ggacggtgac ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt
     5221 gatccgttgc cgtccggttg ccgttctgaa aatgaccgac gaagccggtg aagatgcgaa
     5281 actggttgcg gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa
     5341 cgatctgcct gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct
     5401 cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga
     5461 aatcgttgct tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg
     5521 aacgccgggc ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc
     5581 caccatcatt attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca
     5641 ccattttgct gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt
     5701 caaagcgtgc ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg
     5761 attgcagata tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa
     5821 aatcgcgaga tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac
     5881 ttatccctgt ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga
     5941 taaacgtaca tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg
     6001 cccctggtgc gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca
     6061 ccgtgtaccg ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg
     6121 tagttatcga tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct
     6181 tgtttgtggc gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga
     6241 gcaaatccag tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc
     6301 gataacgtaa cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca
     6361 gacctggcaa tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt
     6421 cgagcgctaa attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt
     6481 gcgcaacccc cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct
     6541 ccagcccttg tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag
     6601 agaagatctc cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat
     6661 cgtcttctgt agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg
     6721 gaccagagaa cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc
     6781 gcccttcagg gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt
     6841 taagatcgcc cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa
     6901 cgtggacaat caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca
     6961 ggttatcgtt aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt
     7021 gcaccacgat gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg
     7081 tagcctcgaa tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc
     7141 gcactttact gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg
     7201 catcaccgtt caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc
     7261 gcataccgtt aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc
     7321 cgtcgatatc cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac
     7381 cacccagacg caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg
     7441 ggtttatcat cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc
     7501 cttgcggatc ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg
     7561 tccagcccaa ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg
     7621 ccaccggcag cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg
     7681 tgatactgcc ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg
     7741 tgaagatacc gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga
     7801 agcggttgag attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc
     7861 acagttccgc attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat
     7921 tgcggtaatc cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc
     7981 gggtattgct aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat
     8041 taagctgccc ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc
     8101 tgcctttggc attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa
     8161 cgcgtacgtc gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa
     8221 cctgcgccac ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt
     8281 gtggcgcttc caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg
     8341 gcagcgcgtt atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc
     8401 ccagctcgcc tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca
     8461 tccactgcat ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga
     8521 cgttaccggt cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac
     8581 cgcgggtttt aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt
     8641 taatgccatt aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg
     8701 ccttgagttc agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct
     8761 gttcattacc tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg
     8821 ccgtacgcat agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat
     8881 ccgcctgata ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca
     8941 cgttgagtgg caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg
     9001 gaccggaaag gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct
     9061 tcaccttttc acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg
     9121 gccagttgtc tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga
     9181 tatccagggc gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc
     9241 gcacggtgat gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt
     9301 tcaggttaag cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat
     9361 ctttcagcgt ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga
     9421 ccacttcttc ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg
     9481 acgtcggttt cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca
     9541 ccgacaccgt ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg
     9601 tgatgggata cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt
     9661 cagaaggagg cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat
     9721 taatacaaac gctactgttc cacaggcact caagaccgac ccccagatgc agattacccg
     9781 cttttaccgc cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc
     9841 cgccggtgac cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa
     9901 gatgcaggcc gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa
     9961 cgataaccac gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat
    10021 gtaaaactgt aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat
    10081 tggcccgacc ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg
    10141 gcgaatatcg cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt
    10201 cacgttgtac tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc
    10261 gttggcgtat ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc
    10321 gacgaagaaa cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag
    10381 cgtgccgcgt gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgtgctgcgc
    10441 ctggaaaacg gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta
    10501 gcgttgcgag tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat
    10561 caccccagga taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag
    10621 gctccagcgc aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc
    10681 caccagcgta gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg
    10741 caccaaataa tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag
    10801 ggtctgttcc ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat
    10861 ccacggcttt ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc
    10921 ggtttcgatg gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt
    10981 ttcgcgcgct ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga
    11041 aagtcggcgg ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg
    11101 caccagattt tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa
    11161 gcggtaacgt tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc
    11221 aatgcccagc tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact
    11281 ggttaaggac tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg
    11341 gcgagtatcg agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac
    11401 atcggtgccg ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg
    11461 ccctttcttt ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc
    11521 tttcagacct tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac
    11581 ttcatcactt tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa
    11641 cccctcgacc tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct
    11701 tacacagcat aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc
    11761 cctggaacgc cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta
    11821 aaccccaaat atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa
    11881 tacgcctctt gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag
    11941 tttatttgat aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc
    12001 gatgcccgta gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg
    12061 aatggagatt gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca
    12121 gttacccggc gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac
    12181 ttatcgggaa gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga
    12241 tccttccgtc atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc
    12301 ccagggaatg cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct
    12361 gaccccagaa caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct
    12421 tgccgccgat gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta
    12481 tgccgaagat gaccaccagc aatatctgca taaaaaccca tatggttatt gtggaattgg
    12541 cggaattggc gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt
    12601 gataaagtgc tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat
    12661 cgcccaaatt caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca
    12721 gtattgcatt tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc
    12781 tatactagcc actgaaaatg ccggttcact ttcttcgaat cggctttcaa tgtgtatttc
    12841 acacaaatta atcaactttc ccttccgagg gtctggcctg aaaggtcgga taagatatgt
    12901 taaacagtat tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg
    12961 agatctcgct tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata
    13021 taaatgccca acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc
    13081 aaatcggtct gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc
    13141 cagcttttca cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct
    13201 ttattctctc tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga
    13261 aacgcatcgg tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct
    13321 tctgcctgta cgtttgcacc ccgctggtgt ggttcttcaa cggcctggcg aacatgatct
    13381 tccgtatttt caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg
    13441 cggtagtgga agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg
    13501 aaaacgtctt tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg
    13561 tgatttggtt tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc
    13621 cgcactctaa gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt
    13681 ctaaagacct gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac
    13741 aaattcgcaa cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt
    13801 ttaaaaccgc aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga
    13861 tcatcaccct caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg
    13921 aagagcagat tgtcgcccgt gatgagaact catggctgat tgacggcggc accccaattg
    13981 acgacgtcat gcgcgtgctg gatattgacg agttcccgca gtcgggcaac tacgaaacca
    14041 tcggcggctt tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat
    14101 tcgccggcta caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg
    14161 tgacccggat cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata
    14221 aagaagaaag cgtcgcgtaa cccccagaaa catcaacggc tcctgaatca ggagccgtct
    14281 tattactgca tagcactttg gttaagccat ctctgtttgc agacgcataa cctgacggtt
    14341 aacttcggac atcactgaca aatgcagctt atccttcact tttgggataa gaatcttacc
    14401 cttatcaaat tcaaaagcgc caatgtcctt gatgtataac cgtccacgga acaggatctt
    14461 tacgtacttc gccacctgaa gtgggttgta ccgttggaaa attttcattg ttatctcctg
    14521 ctgagtatta cgcctgtgcg gtgccacaat cggcccaact attatgaggc gcaaatttta
    14581 atgcttagtg actatagact atccgggcaa tgtttccacc gcgtataact ttttttacct
    14641 aaaggttaca attattcaga attatctttt taccgaagcg cggtcttcag tataagcatt
    14701 catttttcat atgatttgtg cgcttgaccg caaactggca tcacacttgc gggaaattcg
    14761 ataaatagca catatgatta aaactcagac ccaagtggtc ggatcacctg catatcataa
    14821 gaaggaaaca ccatgaccct acgcaagatt ctggcactca cctgcctgct gttgccgatg
    14881 atggcttccg cacatcagtt cgaaatcggt cagcgagtgc cgccgattgg cattaccgat
    14941 cggggcgagt tggtgcttga taaagatcag tttagctaca aaacctggaa cagcgcgcag
    15001 ttagtgggaa aagtgcgagt actgcaacat attgctggtc gcacctctgc aaaagagaaa
    15061 aacgcgacgc tgattgaagc gattaaatca gcgaagttac cgcacgatcg ttaccagacc
    15121 accaccattg ttaacaccga cgacgcgatt ccgggttccg gcatgtttgt gcgcagcagt
    15181 ctggagagta ataaaaagct ttatccctgg tcgcagttta ttgtcgatag caatggcgtc
    15241 gcgcgcggtg cctggcagct ggatgaagaa agttccgctg tagtggtgct ggataaagac
    15301 ggtcgcgtgc aatgggccaa agacggggcg ctcacaccgg aagaggtgca gcaagtgatg
    15361 gacctgctgc aaaagttgct caaataatgg caataacata aattctggaa aaataatttt
    15421 cccctatcgt tatctatggg ataggggaat taagtctctg agttcttaca ccgaactaat
    15481 actggtaacg ctagttgctc ttttttaaac gcctcaatgg tcgatttata tttctcgcaa
    15541 atcttttctt tggtaatatt actatctgcg gccagattcg cacttactat cgcatccact
    15601 tgttcggcta attcgacttg ttggcaacct gcattaacca gaaaattata aataagagag
    15661 taatcttcat cctccaaaac aacagaggaa ccattacgaa aacgttcatc ccctccatag
    15721 attttaagcg caaagcccaa agattctatg actatggcaa aattatcatc aattattttt
    15781 tcgtaaatat accatccatc gcattcttta attccaccgc ctggtatttg gagttcaaat
    15841 ttaccccgtt ttaatttgct tactacatcc tccaataagg cataaccgaa agatacgtca
    15901 ccaaacgcct ggcgaatata acctttaatc ctttttggta tgtcagactg ttttttctgc
    15961 aaaatatcac aggcatacaa tcttctgttt tgttctaacg gaaactcgcg gtttttgaaa
    16021 tccatgaatg agttatactc atttattgtt atgggaattt cttcgtattc aaacccactg
    16081 cctcgcaatg ttattgtata ttcagcaccg ttatctttaa ggctgaaact gataagaaaa
    16141 ttatccatgg cattaacagt ttcatttttt gatgaaagca cttgataact atttttatca
    16201 atctgtgttg cgcaacgaaa ggactcccgc aaagttaaca gactatcata attcgaaacc
    16261 caatcttttt tcttaaatgc atgctgaata ttattaaaaa aatcgtttga atgtgttttt
    16321 gtatgcgatg actttacaaa taactccacc gcaccaataa aattattgtg tctgatcctg
    16381 ggtaacatat ggctacctta ttttatttgg ttattttatg tcgccattaa atatctcatt
    16441 atagaagtaa actatcaaaa aacgctcatt attaaaatgt aacttactag caattgaatt
    16501 tttgcacttt aaatacaatc tctttttatt tataactttt taggttggga taaatcagga
    16561 tggcagaatc aggaaataca ctgtttctgc catctgaatt tagtaaatgg acactctgaa
    16621 ccccggattc aggaacgact cgcgcggagt gtaatccagc ggtttaccct gccagtcgtg
    16681 aacgtgcgct ccggcagctg cagccacagc atgtccggcg gcggtgtccc aaatattcgt
    16741 tggtccgaag cgcgggtaca gctgcgcctg tccttccgcc accaggcaga atttcagcga
    16801 agagccgatg gacgtggtct gatgttcgcc aagctgttgc agatactctt tcagctccgc
    16861 atctgcatgg gaacggctga taaccaccag cggcgggcgc gcatcgcgga cctgaatcag
    16921 cttgcgcaca ccgcactctt ctttccaggc cttgccttct gccgcgctgt acattacgtt
    16981 cattaccggc gcatacacca cacctaaaat cggtttgcca tggtcaatga gcgcaatgtt
    17041 aacggtgaat tcgccattac gtttaataaa ctctttagta ccatccagcg ggtctaccag
    17101 ccagtaacgc tgccagtgct gacggacttc ccaaccggga ggatcttctt cagaaaggac
    17161 cggaatatcc ggtgccagcg tacgtaaacc gtccatgata acggtgtgag cggcaatatc
    17221 cgctgccgtt accggagaat tgtccgcttt gctgacgacg tccatcggtt tcgtcccgtc
    17281 gtagacctgc ataatggcat cgcctgcatt ccgtgcaagc tggcatactt gatctaacat
    17341 ttctccacct cgtctctgtg agcggtgtta acttattgtt ttacttatac cctatcgtta
    17401 atgaatgcgc caactgtgat agtgtcatca ttttcaaagc gtaaaattgt ggcattcttc
    17461 actgttctat aagtaaggcg tttattctcc cttttctttc gtattcccga tgataaaagg
    17521 atgtccctga tgattaagtt tagcgcaacg ctcctggcca cgctgattgc cgccagtgtg
    17581 aatgcagcga cggtcgatct gcgtatcatg gaaaccactg atctgcatag caacatgatg
    17641 gatttcgatt attacaaaga caccgccacg gaaaaattcg gactggtacg tacggcaagc
    17701 ctgattaacg atgcccgcaa tgaagtgaaa aacagcgtac tggtcgataa cggcgatttg
    17761 attcagggga gtccgctggc cgattacatg tcggcgaaag gattaaaagc aggtgatgtt
    17821 catccggttt ataaggcgct gaatacgctg gattatacgg tcggtacact cggcaatcat
    17881 gaatttaact acggtctgga ttacctgaaa aatgcgctgg cgggagcgaa attcccttat
    17941 gtaaatgcca acgtcattga cgccagaacc aaacagccaa tgtttacacc gtatttaatt
    18001 aaagataccg aagtggtcga taaagacgga aaaaaacaga cgctgaagat tggctatatt
    18061 ggcgtcgtgc cgccgcaaat catgggctgg gataaagcta atttatccgg aaaagtgacg
    18121 gtgaatgata ttaccgaaac cgtgcgcaaa tacgtgcctg aaatgcgcga gaaaggtgcc
    18181 gatgtcattg tcgttctggc gcattccggg ctgtctgccg atccgtataa agtaatggcg
    18241 gaaaactcag tttattacct cagtgaaatt ccgggcgtta acgccattat gtttggccat
    18301 gctcacgccg ttttcccggg taaagatttt gctgatatcg aaggggctga tatcaccaaa
    18361 ggcacgctga atggtgttcc ggcggtaatg ccgggcatgt ggggcgatca tcttggggtg
    18421 gtcgacttac aactcagtaa tgacagcggt aaatggcagg tgacgcaggc gaaagcggaa
    18481 gcacggccga tttacgacat cgccaataaa aaatccctcg cggcggaaga cagcaagctg
    18541 gtagaaacac tcaaagccga tcacgatgcc acacgccagt tcgtcagcaa gccaatcggt
    18601 aaatctgccg acaatatgta tagctatctg gcgctggtgc aggacgatcc gaccgtgcaa
    18661 gtcgtgaaca acgcgcaaaa agcgtatgtc gaacattaca ttcagggcga tccggatctg
    18721 gcaaaactgc cggtgctttc agctgccgca ccgtttaaag ttggtggtcg caaaaatgac
    18781 ccggcaagct atgtggaggt ggaaaaaggc cagttgacct tccgtaatgc cgccgatctt
    18841 tatctctacc ccaatacgct gattgtagtg aaagccagcg gtaaagaggt gaaagagtgg
    18901 ctggagtgct ccgccggaca gtttaaccag attgctccta acagcacgaa accacagtca
    18961 ctcatcaact gggatggttt ccgcacttat aactttgatg ttattgatgg tgtgaattat
    19021 cagattgatg ttacccaacc cgcccgctat gacggcgagt gccagatgat taatgccaat
    19081 gcagagcgga ttaagaacct gacctttaat ggcaagccga ttgatccgaa cgctatgttc
    19141 ctggttgcca ccaataacta tcgcgcttac ggcggcaaat ttgccggtac gggcgacagc
    19201 catatcgctt ttgcttcacc ggatgagaac cgctcggtgc tggcagcgtg gattgctgat
    19261 gagtcgaaac gtgcggggga aattcatccg gcggcagata acaactggcg tttagcaccg
    19321 atagctgccg ataagaaact ggatatccgt ttcgaaactt ccccgtcaga taaagccgca
    19381 gcgtttatta aagagaaagg gcagtatccg atgaataaag tcgcgaccga tgatatcggg
    19441 tttgcgattt atcaggtgga tttgagtaag taaaacactt ctttttcggc ctataaatca
    19501 accgcatccg gcatttattg gcggatgcga tgctgccgta tcttatccac cctacaagcc
    19561 atgaaccgta gaccggataa gcgcagcgca tccggcagtt atgccgcacg ttcatcccgc
    19621 accgccaaca cctcgggcaa attcaactca atccagtccg ccagtgcagc aaccttttcg
    19681 ctcacctgct cgcccagcgg cgtgaggcta tattccacat gcggcggcac caccggatac
    19741 gcgatacggt taagaaaacc atcctgttct aacgcctgta acgactgcgc aagcatcttt
    19801 tcactcaccc caccgatttt gcgccgcagg tcgctaaagc gatgagtacc ttcgcgtagc
    19861 gccaccagaa tcaacacccc ccaacggctg gtgacgtgtt tcaacacctc gcgcgacggg
    19921 cactgttccg caaagagatt accctctttc agttgttgtg acaggctaac ctgactcatt
    19981 tcatacttac ctttttgcac gtacttacta aaagtaagtt taggtgttag cgtatttaaa
    20041 cacaagacaa aacgatggag acttcccatg atcgctatta ctggtgccac tggccaactt
    20101 ggtcactata ttattgaatc cttgatgaaa acggttcctg ccagccaaat agtggctatc
    20161 gttcgtaatc cggcaaaagc ccaggccctg gcagcacaag gcattaccgt gcgtcaggct
    20221 gactacagcg atgaagccgc actgacatct gcacttcagg gagtggaaaa actactgctg
    20281 atctcttcca gcgaagtggg tcaacgtgcc ccgcagcatc gtaatgttat taatgccgca
    20341 aaggcggctg gcgtgaaatt tatcgcttat accagcctgc tacatgcaga tacctccccg
    20401 ctcggcctcg ccgatgagca catcgagacg gagaaaatgc tggctgattc tggcatcgtt
    20461 tacaccctgc tgcgcaacgg ctggtacagc gaaaactacc tcgccagcgc cccggcagca
    20521 ctggaacacg gcgtatttat cggtgcggct ggcgatggca aaatcgcctc agcaacgcgg
    20581 gcagattatg cgacagctgc ggcacgcgtg attagcgaag ccggtcacga aggcaaggtt
    20641 tacgaactgg cgggcgatag tgcctggacg ttgacacagt tagcggcaga gctgaccaaa
    20701 cagagcggca aacaggttac ctatcaaaat ctgagcgaag ccgatttcgc cgcggcactg
    20761 aaaagcgtcg gactgcccga cggactggcg gatatgctgg cggattctga cgttggcgca
    20821 tcgaaaggtg gtctgtttga taacagcaaa acgcttagca aattgattgg ctgcccaacg
    20881 acaacgttag ccgaaagcgt aagccatctt tttaatgtta ataactagtt aattaaagtg
    20941 gcatcctccc gcatcctctc tgataatgac gggatgccgg gagcaatcat gtctgcttcc
    21001 tgaacgtttc ttctgacaga ccaatggatg ccagtaatga ttagcggcgt gctgtacgcc
    21061 ctgttagcag ggttgatgtg ggggcttatt tttgtcgggc cgttgatcgt gccggaatac
    21121 ccggcgatgt tgcagtcgat ggggcgttat ctggcgttag ggttaattgc gctacccatt
    21181 gcctggctgg gacgcgtgcg tctgcgtcag ttggcgcgtc gggactggct taccgccttg
    21241 atgctcacta tgatgggcaa cctcatttat tacttctgcc ttgccagtgc cattcaacgt
    21301 actggcgcgc ctgtttccac gatgattatc ggcaccctgc cggtggtgat tcctgtcttt
    21361 gccaatctgc tttatagcca gcgcgacggc aaactcgcgt ggggaaaact cgccccggca
    21421 ctgatttgta ttggcatcgg cctggcgagt gtgaatattg ctgagttaaa ccacggactc
    21481 cccgattttg actgggcacg ttatacctct ggcatcgtgc tagcgttagt ttccgtggtc
    21541 tgctgggcat ggtatgccct gcgcaacgcc cgctggctgc gggaaaatcc cgacaaacat
    21601 ccgatgatgt gggcgacggc gcaggcgctg gtcacgctgc cggtttctct catcggctat
    21661 ctcgtcgcct gttactggct gaatatacaa acgccggact tctccttacc ttttggcccc
    21721 cgtccgctgg tgtttattag tctgatggtt gcgatagccg tgctttgctc atgggttggc
    21781 gcactctgct ggaacgtcgc cagccagcga ttaccgacag tgattctcgg gccgctgatt
    21841 gttttcgaaa cgctggcagg tttgctgtac acctttttac tccgccagca aatgccgccg
    21901 ctaatgacgc tgagcggtat cgcgctgtta gtgattggcg tggtcatcgc ggtcagagca
    21961 aaaccagaaa aacctttaac tgaatctgtc tcagaaagtt gacatgctgg cagtgagtta
    22021 aataagcctc tgctacgtaa gggttatagc ttttgcctta aaggtgtatt taaaatacat
    22081 cttatcttat taagaatgag gtatcagcta tggcttatcg cgaccaacct ttaggtgaac
    22141 tggcgctctc tatccctcgc gcttcggctc tgtttcgtaa atatgatatg gattactgct
    22201 gtggcggtaa gcagacgctg gcgcgcgcgg cggcacgtaa agaactggat gttgaggtca
    22261 ttgaagctga actggcaaag cttgctgaac aaccgattga gaaagactgg cgtagcgccc
    22321 cgctggcaga aatcattgac catatcatcg tgcgctacca cgatcgtcac cgcgagcagc
    22381 tgccggagct gatcctgcaa gcgactaaag tcgagcgcgt tcacgccgac aaaccgagcg
    22441 tgccaaaagg gctgacaaaa tacctgacca tgctgcatga agagctttcc agccacatga
    22501 tgaaagaaga gcagatcctc ttcccgatga tcaaacaagg catgggcagc caggcaatgg
    22561 ggccaatcag cgtaatggaa agcgagcacg atgaagcggg cgaactgctg gaagtgatca
    22621 aacacactac caataacgtc acaccgccgc cagaagcctg caccacctgg aaagcgatgt
    22681 ataacggcat taatgaactg attgatgacc tgatggatca catcagtctg gaaaacaatg
    22741 tactgttccc acgcgcccta gcgggtgagt gatctaaaag ctggatggca ttgcgccatc
    22801 cagcatgata atgcggttat ttccgcagtt cagcagcccg cttcttacca ataaacagcc
    22861 agcccaaccc cagcgcgata aaccacagcg gggtaaccag cagcgcctgg cgagtgtcat
    22921 cttccagtgt cagcaacacg accacgaaca caaagaacgc catacatacc cagcacatca
    22981 gcttgccgag cggcatcttg tagatcgact tttcatgcag atgaggacgc tgtttgcggt
    23041 acaccaggta agagcaaagg ataatcgtcc agacgaacat aaacagaatc gcggaaacgg
    23101 ttgtaatcat cgtgaacgcg ccaatcacgc taggattcac atacaacatc accacgccgc
    23161 cgagcagaca gatacacgag aaggtcaacc ctttcgcggg tactgcgcgc ttagaaagtt
    23221 tagcgaacgc tttcggcgca acaccttcct gcgccagacc aaacagcata cggctggtag
    23281 agaagacacc gctgttagcg gaagacgccg cagaggtcag caccacaaag ttgatcacgc
    23341 tggcagccgc aggcaaacct accaacacaa acagctcaac gaacgggctt ttctccggga
    23401 ctaccgaact ccacggtgtc acggacataa tcacaatcag cgcgaagacg tagaacataa
    23461 tgatacggat cggaatggag ttaatcgcgc gtggcagtga tttctccgga tctttggttt
    23521 ccgcagctgt tgtacctacc agctcaatcc ccacgaaagc gaaaaccgct atctggaatc
    23581 cggcaaagaa gccacttaaa cctttcggga accagccgcc gtcattccac aaatgcgcga
    23641 atgacgcttc cacaccggtc ggtgactgaa agtgcatcgc caccatgacc aggccgacga
    23701 caatcagcga cacgatggcg acgattttga tcatcgcaaa ccagaactcc atctcaccga
    23761 acattttcac ggtggcaagg ttgagcgtca gcagcagcac tatcaccgcc agcgaggcga
    23821 cccagtcgga gagatcgggg aaccagaact gagcataagc cgtgatcgcc accacgtctg
    23881 ccataccggt tacaacccag cagaaccagt aagtccagcc ggtgaaatat cccgcccacg
    23941 gccccagtaa atcggaagcg aagtcactaa aagatttgta ttccagattc gaaagcagca
    24001 attcccccat tgcccgcatc acgaaaaaga gcataaaacc aatgatcata taaacgaaaa
    24061 tgatcgacgg cccggcaagg ctaatcgttt tgccggaccc cataaacaac ccagtaccaa
    24121 tggcacctcc aatggcaata agctgaacat gtcggtttgt gagattgcgc cgtagcgact
    24181 gttcagccgg agcctgatca tcggcaacga cttttacctg atctaccatg ttttttcttc
    24241 ctgtacctgt ctgtgttgtt caggctctac ggcctttagt cagtctatga tacgacgaat
    24301 ctgtatcggg actcatcgat attaggtaac aatacgcggg atgaatacta agatttagat
    24361 ctaatgttaa ttttatgttt aaagtgagtg tcatatcacg gtgataacgc gaaacagctc
    24421 acaaaaatac acgcttacgg ggtctttgca acacaaaata gcgccgtttg gcaaactgaa
    24481 gggtttattg ctgaatgcct gctcccctct ggttcgaggg gaacagaatg cgctgcttag
    24541 aggatttcca gcagttcgac ttcaaacacc agggtgctga acggagggat ggatgcacct
    24601 gcgccgcgct cgccatatgc cagttcctgc gggatagtca gttcccattt agaacctacc
    24661 ggcatcagag tcagtgcttc aatccagcca gggatcacgc cattaaccgg gaattcagcg
    24721 ggttcaccac gagcaacgga gctgtcaaac acggtgccgt cgatcagttt accggtgtaa
    24781 tgaacacgaa cgcggtcggt acgtgccgga attgcgcctt caccctggtt gatcacgcgg
    24841 aattgcaggc cagattcggt gctattcaca ccttcttttt tggcgttttc ttccaggtat
    24901 ttcacgcctt cagcagccat cgcctggaaa cgctgacgac gaacggcatc ggcgcgttcg
    24961 tggatttcac gcagcgcgcg atgcaccaca tcaaccggca cagccggatg tttgccttcc
    25021 agcgcatcgg caatacctgc aaccagtgct tctggcagca gcccttccag gccagattca
    25081 ctcagttgtt gccctacctg caaaccaatg ccgtagcttg cttgcgcttc gatggtgtca
    25141 aaagttgggg tggtcatcgt atttcctttc ttagggtata aaagtagcgg gcagcatatc
    25201 agccacactt ctttgggtaa aactttgtcg caggaatgat gacaaatccc ggggaaaaag
    25261 aaacaatagc aatatcccgt atgaatataa taaaggcggg tttaccacgg acatcatagc
    25321 gttagcttat tcataggcta tgattgagga acaagacgtg gagcaggagg aaaccatgcc
    25381 cgggcgcttt gaactaaaac caaccctgga gaaagtctgg cacgcgccgg acaattttcg
    25441 cttcatggac ccgctgccgc cgatgcatcg tcgggggatc attattgccg ccattgtatt
    25501 ggtggtcgga tttctgcttc catctgatga tacgcccaac gcgtcggtgg tgacgcgtga
    25561 agcgcagtta gatattcagt cgcaatcaca accgccgaca gaagaacagc cgcaaactac
    25621 ccaaacgcaa ccattccagc cagatagcgg aattgataat caatggcgct cttaccgtgt
    25681 cgaacccggt aaaacgatgg cgcagctgtt ccgcgaccac ggactgcccg ctaccgatgt
    25741 ctatgcaatg gcgcaggtag aaggcgcggg taagccgctg agtaatttgc aaaatgggca
    25801 gatggtgaaa atccgccaga acgccagcgg cgtggtgacg ggcttaacca ttgatacggg
    25861 gaataatcaa caggtcctgt ttacccgcca gccggatggc agttttattc gtgcgcggta
    25921 gatgaagggt caaccaaaag ccagctaagg attgtctctt tgatgacctt acgtctggct
    25981 tccagtgcct ggggggaaac catactgata tgataaacta ggccaagcgt atgctggttg
    26041 atcaaataat atcccccaag cgcagcaata ttaatattca cctgcaatgg gtcgatacct
    26101 ggcttaaaaa tattgtattt cttcccttca tccaataagg attccattaa ctgcaagtga
    26161 gcatggttaa tttctaagag acgctgtgat tttgcataat gcacaccttt actttgattt
    26221 tcactatgca caatttttaa aaaccaagga ttagcaatat aataatccca tgtaaaatca
    26281 ataattttct caatggcttt ttgtggcgga agatctgaga aatcaagggc cttttctttt
    26341 tgtcgaatat cgttccaggt atattccagc acttcgataa ataaattttc tttattacca
    26401 aagtggtgat aaaccgtctg tttactacac cctgctgcct ttacaatatt atctactctg
    26461 gccccttcat aaccatattc ggcaaattca ttaaccgcac atagcagcaa tttttccttc
    26521 aggctctgta cgttgctggt atcaatatac ataacgcttc cccacgacct cttcgtggtg
    26581 actatcataa cctgatttga tttcagcttc agaaaaaagg gcaaaaactt gtactttcat
    26641 caggttatca aatgtcctgt gatcgccatc gcatatcaac caactagatg gtttatcttt
    26701 ttatcctcca gtcgcaagct aatcatcacc aaggaaacaa aattcaaaaa agagagctat
    26761 tgtatgaaat ccactcgtcc tgttgccgtg attaccggag ccactcgcgg tattggaaag
    26821 ggctgtgcgc aggagcttgc tcgtggcggg tttaacctgc tgattaacga tcgtcccgat
    26881 gccgacagcg tagaaaaact gcatatcaca caacaagaat gtttggcgga aggcgtggaa
    26941 gtgatttgct tccctgctga tgtcggtgat ctatctctgc atgaggagat gctcgacgcg
    27001 gcgcaaaacc agtgggggcg gctggactgt ctgctcaata acgcggggat ttcagtgaaa
    27061 aaacgcggtg acctcctcga cctcgaacca gacagcttcg atcaaaacat tgccattaac
    27121 acccgcgcgc cgttcttcct tgctcaggca ttcagtaagc gcttactggc acagccgaaa
    27181 ccggaagcgg agctgccgca ccgctcgatt attttcgtca gttccatcaa cgccatcatg
    27241 ctggcgatga atcgcggcga atacaccatt gccaaaaccg ccgtttctgc cgctgctcgt
    27301 ctgtttgctg ctcgcctttg taatgaacaa attggcgttt atgaagtgcg ccctggtctc
    27361 atcaaaactg atatgactat tccggcaacg gcttattacg acgaactgat tgccaaaggt
    27421 ctggtgccgt ggggacgttg gggttatccg gctgatattg cttccaccgt ccgcgcgatg
    27481 gcggaaggta aactgattta cacctgtggt caggcggtcg caatcgacgg cggcttaagt
    27541 atgccgcgct tctgaggctg aatcatgaga gatctgttac aacgcccgga tttattcagt
    27601 atcaataccg cgacactggg ctataaaacg ccgctacccg ccattattga tgcctgcgcc
    27661 gcacgcggta ttggcgcaat tgccccctgg cgcagggagt tgcagggtga agatctccag
    27721 caaatcaccc gccagcttgc cgccagtaac atgagcgttt ccggtttgtg ccgtagcact
    27781 tactacaccg cgccgacact ggcggaacgc aaactggcga ttgatgataa ccgccgggcg
    27841 ctggacgacg cagcggtgct taacgccgcc tgttatatgc aggtggttgg cggtctgcca
    27901 acgggcacta aagatctata tgaagcgcga gaacaagtga aacaagggat tcgccagttg
    27961 ctgccacatt cgaaagatgt cggcgtgcca attgcgctgg agccactgca cccgatgaca
    28021 gctgccgatc gctcttgcct gtgcacgtta cgtcaggcgc tggactggtg tgatgaactc
    28081 gatcccgatg gtgagtttgg cctcggcgtg gcggtggatg tttatcacgt ctggtgggat
    28141 ccggatctcg ccagtcagat cctgcgtgcc ggaaaacgca tcctcgcgtt tcatgtttcc
    28201 gactggttaa tgccgaccac tgatctggtc aatgaccgtg gtatgccggg agatggcgtc
    28261 atcaatattc cgtcaattcg tcggctggtt gaaaacgccg gatttaatgg tgctattgaa
    28321 ctggaaatat tctcgcctta ctggtggcag aaagatatta acag
//
LOCUS       DAJTSA010000051        27564 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000051 DAJTSA010000000
VERSION     DAJTSA010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.051"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            282..1580
                     /gene="kgtP"
                     /locus_tag="OO369_002298"
     CDS             282..1580
                     /gene="kgtP"
                     /locus_tag="OO369_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR5982555.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1577..1900)
                     /locus_tag="OO369_002299"
     CDS             complement(1577..1900)
                     /locus_tag="OO369_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR5982556.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1946..3301)
                     /gene="pssA"
                     /locus_tag="OO369_002300"
     CDS             complement(1946..3301)
                     /gene="pssA"
                     /locus_tag="OO369_002300"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR5982557.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3415..6075)
                     /gene="pat"
                     /locus_tag="OO369_002301"
     CDS             complement(3415..6075)
                     /gene="pat"
                     /locus_tag="OO369_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR5982558.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6107..6805)
                     /gene="tapT"
                     /locus_tag="OO369_002302"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6107..6805)
                     /gene="tapT"
                     /locus_tag="OO369_002302"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR5982559.1"
                     /translation="MTENAVLQLRAERIARATRPFLAHGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6874..7293)
                     /gene="trxC"
                     /locus_tag="OO369_002303"
     CDS             complement(6874..7293)
                     /gene="trxC"
                     /locus_tag="OO369_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR5982560.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7500..8537
                     /locus_tag="OO369_002304"
     CDS             7500..8537
                     /locus_tag="OO369_002304"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5982561.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8585..9274)
                     /gene="ung"
                     /locus_tag="OO369_002305"
     CDS             complement(8585..9274)
                     /gene="ung"
                     /locus_tag="OO369_002305"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR5982562.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9579..9962
                     /gene="grcA"
                     /locus_tag="OO369_002306"
     CDS             9579..9962
                     /gene="grcA"
                     /locus_tag="OO369_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR5982563.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10018..10605)
                     /gene="eamB"
                     /locus_tag="OO369_002307"
     CDS             complement(10018..10605)
                     /gene="eamB"
                     /locus_tag="OO369_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR5982564.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10708..11589
                     /locus_tag="OO369_002308"
     CDS             10708..11589
                     /locus_tag="OO369_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5982565.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11624..12958)
                     /gene="srmB"
                     /locus_tag="OO369_002309"
     CDS             complement(11624..12958)
                     /gene="srmB"
                     /locus_tag="OO369_002309"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR5982566.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13090..13827
                     /gene="trmN"
                     /locus_tag="OO369_002310"
     CDS             13090..13827
                     /gene="trmN"
                     /locus_tag="OO369_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR5982567.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13812..15434)
                     /gene="nadB"
                     /locus_tag="OO369_002311"
                     /pseudo
     CDS             complement(13812..15434)
                     /gene="nadB"
                     /locus_tag="OO369_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15690..15845
                     /gene="rseD"
                     /locus_tag="OO369_002312"
     CDS             15690..15845
                     /gene="rseD"
                     /locus_tag="OO369_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR5982568.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15842..16417
                     /gene="rpoE"
                     /locus_tag="OO369_002313"
     CDS             15842..16417
                     /gene="rpoE"
                     /locus_tag="OO369_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR5982569.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16450..17100
                     /gene="rseA"
                     /locus_tag="OO369_002314"
     CDS             16450..17100
                     /gene="rseA"
                     /locus_tag="OO369_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR5982570.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17100..18056
                     /gene="rseB"
                     /locus_tag="OO369_002315"
     CDS             17100..18056
                     /gene="rseB"
                     /locus_tag="OO369_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR5982571.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18053..18532
                     /gene="rseC"
                     /locus_tag="OO369_002316"
     CDS             18053..18532
                     /gene="rseC"
                     /locus_tag="OO369_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR5982572.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18730..20529
                     /gene="lepA"
                     /locus_tag="OO369_002317"
     CDS             18730..20529
                     /gene="lepA"
                     /locus_tag="OO369_002317"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR5982573.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20545..21519
                     /gene="lepB"
                     /locus_tag="OO369_002318"
     CDS             20545..21519
                     /gene="lepB"
                     /locus_tag="OO369_002318"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR5982574.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21791..22471
                     /gene="rnc"
                     /locus_tag="OO369_002319"
     CDS             21791..22471
                     /gene="rnc"
                     /locus_tag="OO369_002319"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR5982575.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22468..23373
                     /gene="era"
                     /locus_tag="OO369_002320"
     CDS             22468..23373
                     /gene="era"
                     /locus_tag="OO369_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR5982576.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23385..24113
                     /gene="recO"
                     /locus_tag="OO369_002321"
     CDS             23385..24113
                     /gene="recO"
                     /locus_tag="OO369_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR5982577.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24125..24856
                     /gene="pdxJ"
                     /locus_tag="OO369_002322"
     CDS             24125..24856
                     /gene="pdxJ"
                     /locus_tag="OO369_002322"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR5982578.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24856..25236
                     /locus_tag="OO369_002323"
     CDS             24856..25236
                     /locus_tag="OO369_002323"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR5982579.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25656..25736
                     /gene="shoB"
                     /locus_tag="OO369_002324"
     CDS             25656..25736
                     /gene="shoB"
                     /locus_tag="OO369_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR5982580.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25930..26190)
                     /gene="yfhL"
                     /locus_tag="OO369_002325"
     CDS             complement(25930..26190)
                     /gene="yfhL"
                     /locus_tag="OO369_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR5982581.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26405..27501
                     /locus_tag="OO369_002326"
                     /pseudo
     CDS             26405..27501
                     /locus_tag="OO369_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6798 a         6855 c         7227 g         6684 t
ORIGIN      
        1 ttggcgcaga aaaaaatgcc tgatgcgacg ctgcgcgtct tatactccca catatgacag
       61 attcagcaac ggatgcggtt tccccaactt gcctacttcc atacgtgtcc tccttaccag
      121 aaatttatcc ttaagctcct caataaccat tttcctgcta actaaattca tggtttaggt
      181 tgcataatga tacgcaacaa atgtgtaaca ttttctttac caaaagaaat aaacaaaagc
      241 gaccaacaaa agcatcggat tacggcagga gacataatgg catggctgaa agtactgtaa
      301 cggcagacag caaactgaca agtagtgata ctcgtcgccg catttgggcg attgtggggg
      361 cctcttcagg taatctggtc gagtggttcg atttctatgt ctactcgttc tgttcactct
      421 acttcgccca cattttcttc ccttccggga acacgacgac tcaactacta caaacagcag
      481 gtgtttttgc tgcgggattc ctgatgcgcc caataggcgg ttggctattt ggccgcatag
      541 ccgataaaca tggtcgcaaa aaatcgatgc tgttatcggt gtgtatgatg tgtttcggat
      601 cgctggttat cgcctgcctc ccaggttatg aaactatagg tacgtgggct ccggcattat
      661 tgcttctcgc tcgtttattt cagggattat ccgttggcgg agaatatggc accagcgcca
      721 cctatatgag tgaagttgcc gttgaagggc gcaaaggttt ttacgcatca tttcagtatg
      781 tgacgttgat cggcggacaa ctgctagccc tactggttgt cgtggtttta caacacacca
      841 tggaagacgc tgcactcaga gagtggggat ggcgtattcc tttcgcgtta ggagctgtgt
      901 tagctgttgt ggcgttgtgg ttacgtcgtc aattagatga aacttcgcaa caagaaacgc
      961 gcgctttaaa agaagctgga tctctgaaag gattatggcg caatcgccgt gcattcatca
     1021 tggttctcgg ttttaccgct gcgggctccc tttgtttcta taccttcacc acttatatgc
     1081 agaagtatct ggtaaatact gcgggaatgc atgccaacgt ggcgagtggc attatgactg
     1141 ccgcattgtt tgtattcatg cttattcaac cactcattgg cgcgctgtcg gataagattg
     1201 gtcgccgtac ctcaatgtta tgtttcggtt cgctggcagc catttttacc gttcctattc
     1261 tctcagcatt gcaaaacgtt tcctcgcctt atgccgcttt tggtctggtg atgtgtgccc
     1321 tgctgatagt gagtttttat acatcaatca gtggaatact gaaggctgag atgttcccag
     1381 cacaggttcg cgcattaggc gttggtctgt catatgcggt cgctaatgct atatttggtg
     1441 gttcggcgga gtacgtagcg ttgtcgctga aatcaatagg aatggaaaca gccttcttct
     1501 ggtatgtgac cttgatggcc gtggtggcgt ttctggtttc tttgatgcta catcgcaaag
     1561 ggaaggggat gcgtctttag tgacgggtca gttgccagac ggtatagccg gtgcttgcac
     1621 cggcgacatc ccaggccaaa tccttccagc tccagccgct cccttcgggg cggctatccc
     1681 aaagctcttt tgacgccccc aaactgacag agaacatcaa tccaaacatg gcactgcgat
     1741 cccggctcat cccctgatgc tgtgaatatt catttccggc ggcggaaagt atcgccgagg
     1801 cgataaagtg ttgagcttta tcctgcccac tccagctatc gttatccata tggctgcatt
     1861 cagaaagtaa caatagcgaa cagacaaaaa gaatacgcaa aacgtggcct ccaaaaaaca
     1921 aaccccgtac aggacggggt tgtgattaca ggatgcggct aattaatcgg tcgatgcgga
     1981 tacggcgcaa acggcggatg agtttacgaa ccttcaccgg ataatcggca atactttgca
     2041 gatcgcgata gtgcttaacg atggtggtac gctcgcggat cagctccagt tctttctctc
     2101 gctgcggcgc aagctccagt tgtggatcgt ggatcaaaat ggcgttttcc agatccagac
     2161 gccaggcgcg cgggttcagg ttattaccgg tgatcaacat ccacttatca tcaacccaca
     2221 tccctttcag gtgataggtg ttgtcgtcat ctttccataa ccgaaccact agctggtcag
     2281 tattgacgta atactgcaaa cggctcagga aacgacgcag attgatctca tagagataag
     2341 gcaatgcgcc aattatcttg aaaggttcat cttccggaat gtagaagtcg ttcgcggttt
     2401 tatcaccaac aataatttcg acctttttcc cttcgcgcag caactggata atattgcgca
     2461 caaggattgc tggcaggttg aagtatggcg tacagatggt tagtttctgc tcggcacaag
     2521 gcataagatg gaaaatggtc ttgttcaaca gactcgattt ccccagcccc actagcggcg
     2581 ttacggaaag ctgatcgttg tcggcatcgc cctggaaatg ataagcggca tcacgcagct
     2641 cctggcggaa cagacgaata tcgttcttga tttccgggct ttttggccga ttaacatcat
     2701 ccagacgatt aacgccgcgg ccattcataa tattctgtgt aacccattca aacataatgt
     2761 ctgacatctt acggttacgg atcagatgat aacggtcgta gcgatattta tcgtgttgat
     2821 gcaggtaaac atcgttcagg ctggcaccgc tataaagtac gctatcgtcg atgataaagc
     2881 ctttaaagtg cagaacacca agagcttcac gggtattgat tggaacgcca taaaccggaa
     2941 catctacgcc cggattttcc tgcgccatgc ggcagtacca gtcagcgtta gtgttagatg
     3001 ccgcagcgcc aatgcgtcca cgttgtgcac gatgccagtc gaccagcacc cgcacatcca
     3061 gttccggacg ctgcctttta gcctcataca acgcgttcag aatgcctttg ccaccgtcat
     3121 cctgttcgag atacagggcg acaatgcaaa tgcgctgctt cgcgctggct attttttcca
     3181 gcagcgtctc ccggaagtcg gcgggagcgt aaaagaaatc gacatcatca actgattgag
     3241 aaatcttggg tagttgggca aggtgttgtt gatgtttatt acgcttaaat tttgacaaca
     3301 tcacagtgca tttcttctct gttcattgaa gggtcctctg tgcaatgcag acgacataag
     3361 cgggcaataa taacaccagt cccgattaag tggtcaacat ttccagtacc ttactcatga
     3421 ttcctcgcgc cgggcaagat ttagcgtaag cccaacgatc ccctcttcga gctggatatc
     3481 aacgttaaac ccgagcttgc gggctagcgc caccatgcca cgattgtttg gcatcgtaat
     3541 accattcaga cgttgtagtc cgtgatttcg cgtataggta atcaactttt ccattaagcg
     3601 tcgacctaag cctaaccctt tgagatccga gcgaaccagt acagcaaatt cggcatcgat
     3661 gttatcagga tcggaaatcg cacgcgtgac gccgaggatc tcttccgttt gatcaatacg
     3721 tcgtaccgct acaaacgcca tttcccgatc gtagtcgatc tgtgtcatgt tggctaaatc
     3781 ttcatgggta aattcgttga tctcgctaaa gtagcggtaa taaagatctt ctttggtgac
     3841 tcgcgaaatg aattgctgaa gttgtggctc atcttctggc aaaatcgggc ggaacaagca
     3901 gcgttcaccg tttttcaatt ctacccattc ttccagctga tgcggataag ggcgcactgc
     3961 cagccgactc tcgttatcgc cttcaaacgg cgagatatcc agcgtgacat ccagcgcggt
     4021 aaattcactg ccagaagcca gcaaaggatg aatatccaga cgctgaattt ccgggcaatc
     4081 gacaatcaag ttggaaacct gcaccagaag ctggctcaag cctgcaacat ccaatgggcg
     4141 tagcgcactg cgcgcacgaa tctttttact tttgatcccc tgaataacca gatagcgggc
     4201 caggttcatg ttcagcggcg gcagtgcgac gacggcttga tcttcaggac gccactccac
     4261 accgccttca cccagcatga tcaacggccc gaaaaccgga tcgtgctcaa ccacaacccg
     4321 caactcctga gcgccagcac ggttagccat actttgcacc aacaggccgt ggacccgcgc
     4381 ctgtggccag gccattttta cgcgatcgaa aatagcgttc gccgcttgct ggacttcatt
     4441 ggctgtacgc aggtaaagca tgacgccctg aacttccgat ttatgtggaa tatccggcga
     4501 acgcaatttc agcgccaccg gataaccaat ctgttcggca atatgcaccg cttcggtgct
     4561 atcgctggca atccaggttg ggagcgtgtt catgccatac gcttgcagga tgggctgaac
     4621 ttcatgggta tcgagcgacg tagccccttc ggcaatcgcc tgttgcaaca gaagatgcgc
     4681 ttctgcggta ttggaagtca gattgctggg caacgccggc gtttcgcgta gttgcttctg
     4741 attacgccgg tactccacca tatgcataaa agcagtgatg gttccttccg gggtacggta
     4801 ggtcggcagc ccggcttcgc tgaataaacg tcgtgcctct tgcgaggagt gctcgccgca
     4861 ccagttcgtc agcaaagaga catatttgct gcggggatga tgctttaccg cttcaattaa
     4921 tacttgcgcg ctttctgttg cgggagcagc ggcgctgggc gaatgaataa ccatcagtgc
     4981 atcaaaatcc tggctgtgga gcagaatgtc cagcgttttg acataatgct cgctgctggc
     5041 gtcatcgcgc agatcgagcg ggttagagac tgccacatgt tctggcaatg catcgcgcaa
     5101 tttctggcag gtttcttcgc ttagcgttgc cagcttgcca ttgcgtgacc ataaggcatc
     5161 cagcgccagc gcggcaggcg cagcaccgtt gctgataatc atcaaccggt cgccacgcag
     5221 cgggcgcata tggctaaggg tttccaccgc cgaaaacagt tcgtgggtat cctgtacccg
     5281 caacaaaccg gcacgctgaa tagccgcatc ccatgccggg tccattcctg ccgtggtgtt
     5341 gagcagtcgc tgtgccgccg ggctacgtcc gcttttaatc accagaatcg gtttattacg
     5401 cgaggcacta cgggccgccg aaacaaagcg tcgcgcgtcg cttaactgtt cgagatagag
     5461 caggatggcg ctggttttac tgtcgcgcgc cagatagtcg agcaattcat caacgtcgat
     5521 atctaggctg tcgccgagcg caataaagta ggaaaagccc atctcacgct gttgcgccca
     5581 gtcgaggatg gtgttggaga cggcagccga ttgcgaaata aacgccagct tgccgcgttt
     5641 aatcggcaca ggcgaaaagc tggcattcag accttgccag ggagccagta aacccagact
     5701 gtttggtcca agcaggcgca tgttatggcg cagggcgcag gcgcggagat cttcgtgttg
     5761 cgatgccggg gcggaaagaa taatgcaggt tttacagcct ttctcgccga gctcttccag
     5821 aagagcaaga ttacggctgg cattggtaca taaaaccgca aggtcgggtg taaagggcaa
     5881 gctggcaata tccggccagg ccaacacacc caacaccgct ttccaggctg gcgtcaccgg
     5941 gagtaccggt ccgttaaagc ctcccgccag caggttacgc atcatcaggt aacctgcgcg
     6001 attgggtttc atcgacgcgc caattaccgc tatcgatttt ggtcgcagta gtgcttccag
     6061 tcctcgctga ctcataccgg tctcccgcag aagtgaccga atgattttaa acgctttcta
     6121 actgttctgc tgtgatgcta cccagatgtt gcgtttttcc tgccagatag cgtgttttaa
     6181 agcgggtaaa atgctcgcct aaccctgctg ccgccccggt atcgccggcc atatctaaca
     6241 gtgcgatggc tacctcggca gtacaatatt ggcctgcagc ctgggcttca cgcaggcgat
     6301 aggcagaaag ccgggaaaga tcgacggaaa tgacgggaag attatccaga tacggacttt
     6361 tacgaaacat cttgcgagct tccggccagg taccatcgag catgataaac agcggtggct
     6421 taccggcagg tggtgtgaag atcacttccc gttgctcatc agcatacgag gcgggaaaga
     6481 ccaccattgg ctgataatcc gggttttgta ccagatccag caaatcctgc gagggttcgg
     6541 tacgcgacca ttgaaacgca acggtatcag gcaaaatatc agcaatcaga cgcccggtat
     6601 tactgggctt cattggctcg gtgtcgaaca tcagcaaaca gaagcgactt tttgcttgtg
     6661 ctggggtaat tgtcgaacag agacataatt tctctggcaa aagacagcgt tggcagcgac
     6721 gaacgcgatt accgtgggca agaaaaggac gtgttgcgcg cgcaatacgc tcggcgcgta
     6781 actggagaac agcgttttcg gtcataagag agcgtcgaaa aaacgccatt gtcgcagagg
     6841 agaaaacggg gcacaagatg cgccccggta agattaaaga gattcgttca gccagctatc
     6901 gaacggcgct ttcggtactg cgccattaag catgtcgaca acctgaccgt ttttgaaaat
     6961 catgatcgtc ggtatactac gaattccaaa gcgactgctc aattcacgtt cagcttcggt
     7021 attcactttc acaaagcgca ctttaccgct acgctcttgc gcgacatctt caaaaattgg
     7081 tgcgaaatta cggcaggggc cgcaccacgg tgcccagaag tcgatcacca caggtagatc
     7141 atccttcagc aatttgtcga gtgtttcacc ggtcgcatta atcacctctc cgtcaaacaa
     7201 gtcgtgaccg cagcgtccgc attttgccgc atcttcgatc cgatcgtcgg gaatgcgatt
     7261 gatggcctga caatgggtac aaacggtatt cataactaac ctcgggatga gtaaggagac
     7321 ttatcggcaa cgacgccggt atgtttctaa tatgttacat attatcgggg acattgttcc
     7381 agacaacaat ctgttttatt caataggtat gatagtcgca ggctttgcta cgaaatcatg
     7441 gcgaaccgcg cggacttcgg gtaatctgcg cgcttcgcgc agcgctggtg gagaaaagca
     7501 tgaacgatga aatgaaaggt aaaagcggca aggtcaaagt gatgtatgtc cgcagtgatg
     7561 atgattctga taaacgtacc cacaacccgc gtaccgggaa agggggcggg cgtccaggaa
     7621 aatctcgtgc tgatggtggc cgtcgccccg cccgcgatga caaacagagt cagccccgtg
     7681 accgcaagtg ggaagattcg ccgtggcgca cggtttcccg cgcgccgggt gatgagacgc
     7741 cggaaaaggc cgatcacggt ggcatcagtg gtaaaagttt tattgatccg gaagtgttgc
     7801 gtcgtcagcg tgcggaagaa acccgcgtct acggcgaaaa cgcctgtcag gcactgttcc
     7861 agagccgtcc ggaagcgatt gttcgcgcct ggtttatcca gagtgtaacg ccgcgtttta
     7921 aagaagcctt gcgctggatg gcagcaaacc gcaaagcgta ccatgtggtg gatgaagcgg
     7981 aattgacaaa agcgtcaggt acggaacatc acggcggcgt ttgcttcctg atcaaaaagc
     8041 gtaacggtac aaccgtgcag cagtgggtaa tccaggcagg cgcgcaggat tgcgttctgg
     8101 cactggaaaa cgaatctaac ccgcataacc tgggtggcat gatgcgcagc tgcgcgcact
     8161 tcggcgtgaa aggtgtcgtg gtgcaggatg cggcactgct ggagtcgggg gcggctatcc
     8221 gtaccgcaga aggcggcgca gagcacgttc agccgattac tggcgacaac attgttaacg
     8281 tgctggatga tttccgtcag gcaggttaca ccgtagtgac cacttccagc gagcagggta
     8341 aaccgctgtt caaaaccagt ctgccagcga aaatggtact ggtgctgggt caggaatatg
     8401 aagggttacc ggatgccgca cgcgatccga acgatctgcg cgtgaagatt gacggtacgg
     8461 gcaacgttgc cgggctgaat atctctgtcg caaccggcgt tttgcttggt gaatggtggc
     8521 gtcagaataa agcctgataa atcagccggg tggcaactct gccatccggc atttccccgc
     8581 aaatttactc acactctgcc ggtaatactg gcatccagtc aatcggcgtc tcgccacgtt
     8641 gttccagcca ctgatttgcc agcacaaaat ggttgcagcc aaagaatcca cgatgcgccg
     8701 aaagcggcga cggatgcggt gctttcagta catgatggcg ttgcttatct ataatcgccc
     8761 ctttcttttg cgcatgtgat ccccacaaca aaaacaccac gccttcgcgg tgctggttaa
     8821 tcaggctgat gactttatca gtaaacgtct cccaaccgag gctggcgtgg gaatgcgcct
     8881 gacctgcgcg aaccgtcaac acagtattga gcagcagaac gccctgacgc gcccagcttt
     8941 cgagatagcc atgattaggg cgggtgaagc ccggaatagt attttccagc tctttataca
     9001 tattcaataa tgacggagga atggcaatgc cgggacgaac ggaaaatgcc agaccatgcg
     9061 cctgtcccgg tccgtgataa ggatcctggc cgagaatcac cactttaacg tcacccaact
     9121 cggtaaagcg gaacgcgtta aagacatctt tttgtggtgg atagatagtg acgccggact
     9181 gccgctcgct ggcgacggtc tgaagggtat taagaaaata aggttgttgc ttctcttcag
     9241 ccagcacgtc atgccaggtt aattcgttag ccatcttgct ctcctgcgaa tcttcaatcc
     9301 gcctagctta actgcttctt tctacagaac aaaatcatgc tcatttttta gacttaaacg
     9361 agtgcaaaaa aatgtaaatt ggtaaaattt cacacccgca taagttgatg taaaacaata
     9421 aaatccattt acgaccacaa tcgatgtgtg gtttttgttg atttaaatca aagaatcaag
     9481 ggtggttgag gagtatatat acactcaagc aataatggtt ttaccaattg gccgcgacag
     9541 gctgaacaaa tcaaataatt ttgccgggga ggcatcacat gattacaggt atccagatta
     9601 ctaaagccgc taacgccgat ctgctgaact ctttctggct gctggacagc gaaaaaggcg
     9661 aagcacgttg catcgttgca aaagcaggtt atgcagaaga tgaagtggtt gcagtaagca
     9721 aactgggtga cattgaatac cgtgaagttc cagtagaagt gaaaccagaa gttcgcgttg
     9781 aaggtggtca acacctgaac gttaacgttc tgcgtcgtga aactctggaa gatgcagtta
     9841 agcatccgga aaaatatccg cagctgacca tccgtgtatc cggttatgca gttcgcttta
     9901 actctctgac tccggaacag cagcgcgacg ttatcgctcg tacctttact gaaagcctgt
     9961 aaggcttaag tagcaagtta ctaagcggaa gagggcgacc tcttccgctt tttttcgtta
    10021 atagaaaatg cgtaccgcgc aatagaccag caacagggca agcacgatat ttaactggcg
    10081 accatactgg cgaaacaatc gctgaaacag atgccccgcc agcgcccagc acacattgcc
    10141 aaacgtccca atcatcgcca gcaaaacgct gacgccaact acccagctta gcgcctgtgt
    10201 ttgcggcaga acaaacgtcg acagtgccgt aacaccgtac aaaatgattt tgacgttcac
    10261 aaactgcaaa gcaaagctgg cccaaaagct gattggtttt gcctgaagtc cgtcttcctt
    10321 tgttgggctg gtggcgattt tccacgccag ccagacaata tatgccgccc ccgcccaact
    10381 caaaaggtgt accgctgccg ggtcaatcac tgccagtgaa aatgaaatgc ccgcacacag
    10441 taacatcaca atcaaaaatc ccagactcat ccctgccagc acgcgggtac tttgacgaaa
    10501 tccatgcgac gtagcagagc taagggcgag aatattgttc ggtcctggcg tcatagcggt
    10561 aatcagggtg taagtccaaa aagcacttaa aaggatcggt gtcacttgtt atctcctctg
    10621 ttttggtaga cagtacccga cacggaacat ttaaaaaaat gaatgttttt atattctaaa
    10681 ttcgatattt tcgataggtt tggggttatg gatctgcgcc gttttattac gcttaaaacc
    10741 gtggtggaag agggttcctt tttgcgagct tcgcaaaaat tgtgctgtac acaatcgacg
    10801 gtgacttttc atattcagca gcttgagcag gagttttcag tccagttatt tgagaaaatt
    10861 ggccgacgga tgtgccttac ccgcgaagga aaaaagttgc tgccgcacat ttatgaactt
    10921 acccgagtga tggatacgct tcgtgaggct gccaaaaagg agtcagatcc ggacggggaa
    10981 ctacgcgttg tttcgggcga aacgttgctc tcttaccgaa tgccgcaggt gttgcagcgt
    11041 tttcggcaac gcgcaccaaa agtgcgttta tcgttgcagg cgctgaactg ttacgtgatc
    11101 cgtgatgcgc tactgaatga tgaggccgat gtcggcgtct tttatcgcgt agggaatgat
    11161 gatgccctga accgacgaga gttgggtgaa caatcgctgg tgctggtggc ttcaccacaa
    11221 attgcagatg tcgattttac tgagccggga agacataacg cctgtagctt tattatcaac
    11281 gaaccgcaat gtgtcttccg gcagatattt gagagcacgc tgcgacagcg gcggatcacg
    11341 gtggaaaaca ccattgagct tataagtatc gaaagcatca agcgttgtgt cgcggcgaat
    11401 atcggtgtca gttacttgcc ccactttgcg gtggaaaaag agattgagag cggagaatta
    11461 attgaattgc cttttggcga acagtcgcag accattacgg caatgtgcgc acattatgct
    11521 ggaaaagcgg ttagcccggc gatgcacatt tttattcagt gtgttgaaga gtgttttgtg
    11581 gttgcgtaaa aaaacaatgc cgggcttaaa cccggcattg agattactct tctgtcgttt
    11641 gcggtggcac gcccgttccg ctcggtttac ggcgcttacc aatatttttg gtgtcgcgat
    11701 ggcgtttttt cacccgcggc ttctctttct ctttagcttt tttcttctcg gcgcgttttg
    11761 ccagtacttt cttcgatggc ttgccggtct gcttctcgct tggcgcacgc gttttcgggc
    11821 gtaactcatc aataacgcga gctttaatcg gctcttcaat gtaacggcca accttgccca
    11881 gcagcaaatg gtcatgggct tccaccagcg aaattgcggt gcctttacga ccggcgcgcg
    11941 cggtacgtcc gatacgatgc aaataagtat cgccactgcg cggcatatcg aagttaaaga
    12001 cgtggctgac gtcaggaatg tcgataccgc gcgcggcaac atcggtggca accagtacgt
    12061 ttacgcggcc ttcggtcagg cgtttaattg cttcgttacg cttgccttgt accatctcac
    12121 cttcgagata gcagttgttg atgcccgctt cgcgcagcca gtttgccagc tcatgcacac
    12181 gttcacgctt acgcacaaac acgattgagc gagtcgcttc aggctgtttt aacaggtgca
    12241 ccagcaatgc ggttttatgt tccaggtcat cggcgcggta ataccactga tgaatttttt
    12301 tgcgctcacg ggtggacgga ttggcagaaa cttccaccgg atcttccagc agacgctcgg
    12361 caaagtcctg aatcgcatcg ccttccagcg ttgccgaaaa gagcagggtc tgtttacgcc
    12421 agcgagtttc gccagcaata tgttcgatat cctgagcgaa gcccatatcc agcatacggt
    12481 ctgcttcgtc gaggatcagc gtttcaaccg cgcggcaatc gaagttctct tcttttatgt
    12541 attgcagcag acgtccggtc gtggcgacca cgatgtcctg attttcgctg aacacttccg
    12601 cgtggttcat ataggctacg ccgccggtga tggtggcgat atccagatgc gtatgtttcg
    12661 ccagttcgcg ggcatgatcg gccacctgca tcgccagctc gcgagttggg gtgaggatca
    12721 aaatacgcgg cggaccggat ttcttacgtg ggaaatcgag caggtgctgc aacgctggca
    12781 gcagatacgc cgccgtttta ccggtgcctg tcggcgcaga accgagtaca tcacggccat
    12841 cgagcgcagg cggaatggcg gcagcctgaa tggcggtcgg gcgagtgaaa cctttatcct
    12901 ggagggcttc cagcaggctt tcgtcgagtt caagttcgga aaaagtcgtt acagtcatgt
    12961 tctacctctg tgtggggcgc tgattataga cgttacggct gcaatcttca tctgtttgta
    13021 tggatatcgc ttctcgggta ttgttctcac ccggtgctat cgccgttttt cctgcaaggt
    13081 tttattttca tgtcacagtc tacatccgtg cttcgtcgta atggatttac ttttaaacag
    13141 ttttttgttg ctcacgatcg ctgtgcgatg aaagtgggaa cggatggcat tttgctgggc
    13201 gcatgggcac cggtggctgg ggtgaaacgt tgccttgata tcggcgcggg tagcgggttg
    13261 ctggcactga tgctggcgca gcgaaccagt gacagcgtga taattgatgc cgttgagctg
    13321 gaaagtgaag ctgccgcgca ggcgcaggaa aatatcaacc agtccccgtg ggcagagcgg
    13381 attaacgtcc atacggcgga tattcagcag tggatcacac agcagacagt acgcttcgat
    13441 ttaatcatca gtaacccacc ttactatcag cagggagtag agtgcgcgac acctcaacgg
    13501 gaacaggctc gctataccac tacgcttgat cacccatcgc tgctgacctg tgcggcggag
    13561 tgcattaccg aagagggatt tttctgcgtg gtgctgccag agcagattgg taatggtttt
    13621 actgaactgg cattaagcat gggctggcat ttacgtttgc ggacggatgt ggcggaaaac
    13681 gaagcgcgac tgccgcatcg ggtgctgctg gcattctccc cgcaggcggg agaatgcttt
    13741 agcgatcgct tagtcattcg tgggccagac cagaactatt ccgaagcgta tacggcgctg
    13801 acccaggctt tttatctgtt tatgtaatga ttgccggggg aaaggatcga tggaccggaa
    13861 tgcgtgagca gttccgggta atccagcgta aaatgcagcc cccggctttc tttgcgcatc
    13921 atcgcgcagc gaacaatcaa ctcggcaacc tgtaccagat tacgcagctc cagcaaatta
    13981 tttgagacgc ggaaatgggc gtaatattcg tctatttctt gttggagcat ggttatccgc
    14041 cgcagggcgc gttccaggcg cttcgttgtg cgcacaatgc caacgtaatc ccacataaac
    14101 agccgtagct cgtgccagtt atgctgaatt actacccgtt cgtcagggtt ctcaacgcgg
    14161 ctttcatccc acggcggtaa cgtactgacg ccgtgggcat aaggcatacg tctggtgata
    14221 tcttctgccg ccgaccagcc atagaccaga cactccagca atgaattcga ggccatgcgg
    14281 ttagcgccgt gtaagccggt ataactcacc tcgccaatgg catacaagcc ctcgacgtcc
    14341 gtacgcccat gatcatcaac cattacacca cctcaggtat agtgtgcagc aggcacaatc
    14401 ggtaccggtt cttgtgtgag atcaatcccc agcccgagca atttttcata aatcatcggg
    14461 aaatgctggc gaataaaatc ggcgggctta tggctgatgt cgaggaacat acaatctgcg
    14521 ccgaggcgtt tcatttcatg gtcaatggcg cgggcgacaa tatcgcgcgg ggccagttcg
    14581 ccgcgctcat caaaatcggg cataaaacgc gtgccatccg ggcgcttgag ataagcgcct
    14641 tcgccgcgca gtgcttccgt taacaggaaa ttgcgtgcct gtgggtgata cagtgcggta
    14701 gggtggaact gattaaattc gagattggca acccgacagc ccgcgcgcca cgccatagca
    14761 atgccatcgc cagaagaaat atccggattg gtggtgtact gatacacctt cgacgcaccg
    14821 ccggttgcca gtaccactgc ttttgcgtgg caggtttcca ccgtttcttt atttcggttc
    14881 catacccacg cgcctacaac ccgtcgcgtg cccggcaggc caattttgtc agagacaatc
    14941 agatcaactg cgttgctgcg ctccagcaca cgaatattcg gatggttctg cgccttgctc
    15001 accagcgtgg attgtacttc tctaccggtg gcgtcggcgg catgaagaat acgacggtga
    15061 ctatgtccac cttcacgggt cagatggtag ctttcttcgc cattcggttg aacgtgggta
    15121 tcaaacaaaa ccccctggtc aattaaccat ggcacacagg aacgagcatt gctggcgaca
    15181 aattcaactg catggtgatc gcaaatacca gccccggcaa tcaatgtgtc ttccacatgc
    15241 gagtcaatgc tgtcagtttc atcaaacacg gcggcaatac cgccctgggc ataaaatgtt
    15301 gaaccttccg ttaccgggcc tttacttaga acgatgacct gatgctggtc agccaggcgt
    15361 agcgccagtg aaagtccggc tgcgccgcta ccaataatca acacgtcaca tgaatgttca
    15421 gggagagtat tcattttctt tgtttaattt actaaacatt gtttggtcag catagcatca
    15481 tgttgtgcga ataaacacct gctattttaa tatttgttac agtggctaaa cacgttgacg
    15541 cagggcggcg agaaaaagag aagttactgg ctggtggagg attaggtggt gaaataaaaa
    15601 ggccgttggg ttactcttca ggcagttaaa tgggcatttc tacacagata atgcgatgtt
    15661 cagattctgt agacttataa tgatagataa tgatccgtct acagcatgac aaacaaaaac
    15721 agatgcgtta cggaacttta caaaaacgag acactctaac cctttgcttg ctcaaattgc
    15781 agctaatgga gtggcgtttc gatagcgcgt ggaaatttgg tttggggaga ctttacctcg
    15841 gatgagcgag cagttaacgg accaggtcct ggttgaacgg gtccagaagg gagatcagaa
    15901 agcctttaac ttactggtag tgcgctatca gcataaagtg gcgagtctgg tttcccgcta
    15961 tgtgccgtcg ggtgatgttc ccgatgtggt acaagaagct tttattaaag cctatcgtgc
    16021 gctggattcg ttccggggag atagcgcttt ttatacatgg ctgtatcgga ttgctgtaaa
    16081 tacagcgaaa aattacctgg ttgctcaggg gcgtcgtcca ccttccagtg atgtggatgc
    16141 cattgaagct gaaaacttcg aaagtggcgg cgcgttgaaa gaaatttcga accctgagaa
    16201 cttaatgttg tcagaagaac tgagacagat agttttccga actattgagt ccctcccgga
    16261 agatttgcgc atggcaataa ccttgcggga gctggatggc ctgagctatg aagagatagc
    16321 cgctatcatg gattgtccgg taggtacggt gcgttcacgt atcttccgag cgagggaagc
    16381 tattgataac aaagttcaac cgcttatcag gcgttgacga tagcgggata ctggataagg
    16441 gtattaggca tgcagaaaga acaactttcc gctttaatgg atggcgaaac gctggatagt
    16501 gagctgctta acgaactggc tcataaccca gaaatgcaga aaacctggga aagctatcac
    16561 ttaatccgtg attcaatgcg gggtgatact cccgaggtgc tccatttcga tatctcttca
    16621 cgcgtgatgg ccgccattga agaagagcca gtacgtcaat cggcgacatt gatcccggaa
    16681 gcccagcctg cgccgcatca atggcagaaa atgccattct ggcagaaagt acgtccgtgg
    16741 gcggcacagc ttacccaaat gggcgtagcc gcatgcgtat cgcttgcagt tatcgttggc
    16801 gtccagcact ataatggaca atctgaaacg tcccagcagc ccgaaacgcc ggtatttaat
    16861 acactgccga tgatgggtaa agccagcccg gtaagcctgg gagtaccttc tgaagcgacc
    16921 gcaaacaatg gtcaacagca gcaggtacag gagcagcgtc gtcgcattaa tgcaatgttg
    16981 caggattacg aactgcaacg ccgactccac tctgaacagc ttcagtttga gcaggcgcaa
    17041 acccagcaag ccgctgtaca ggtgccagga attcaaactt taggaacgca atcgcagtaa
    17101 tgaagcaact ttggtttgcc atgtcattag tgacaggtag cctgttattc tctgctaacg
    17161 cctcggccac tcccgcgtcc ggggcgttat tacagcagat gaacctggcc agtcagtcac
    17221 tgaattacga gctgtcattc atcagcatca ataaacaggg tgttgagtct ctgcgttatc
    17281 gacatgcacg cctcgataac cgtcctcttg cacaattgtt gcaaatggat ggcccgcgcc
    17341 gggaagtggt acagcgcggc aatgaaatca gctattttga accgggactt gaaccgttca
    17401 cgcttaatgg cgattacatt gttgattctc tgccatcgct tatctatacc gatttcaaac
    17461 gcctttctcc ttactacgac tttatctccg tcgggcgcac gcgtattgct gatcgtcttt
    17521 gcgaagtcat tcgcgtggtt gcccgagatg gtacacgcta cagctacatc gtgtggatgg
    17581 acaccgaatc gaaattaccg atgcgggttg atctccttga tcgcgatggt gaaacgctgg
    17641 aacaatttcg cgtgattgct tttaacgtca atcaggatat cagcagcagt atgcagacgc
    17701 tggcgaaggc aaatttgccg ccgttgcttt ctgttcctgt aggtgaaaaa gctaaattca
    17761 gctggacgcc aacctggttg ccacagggtt ttagcgaagt ttccagtagt cgacgtccgc
    17821 taccgacgat ggacaacatg cctatcgaat cacgtctcta ttccgacgga ttattcagct
    17881 tctcggtaaa cgttaaccgc gctacgccat cgagcaccga tcagatgttg cgcaccggac
    17941 gcagaaccgt cagtacaagc gtacgtgata acgccgaaat caccattgtc ggtgaactgc
    18001 cgccgcaaac ggcgaaacgc attgccgaga atattaagtt cggggcagcg caatgatcaa
    18061 agagtgggct accgtcgtct cctggcaaaa cgggcaggcg ctggtcagtt gtgatgttaa
    18121 agcttcatgc agcagctgcg cttcacgcgc cggttgcggt agccgcgtgt tgaataaact
    18181 tggcccgcaa accacgcata ccattgtcgt accctgtgat gagccgttag tgccggggca
    18241 aaaagtggag ttggggatcg ccgaaggcag cctgcttagc tccgcattac tggtttatat
    18301 gtcgccgctg gtgggattat tcctcatcgc ttcgctattt cagctactct ttgcttctga
    18361 cgtcgcagca ttatgtggtg cggtcctcgg cggtattggc gggttcctga ttgcgcgcgg
    18421 ctactcgcgt aagtttgctg cccgggcaga atggcaaccg atcatcttaa gcgtggcatt
    18481 gccgcctggc cttgtgcgat ttgaaacatc ttcggaagac gcgagccagt gatttcactg
    18541 gctttgttgc attttgcata acaaatgcga ggacgtttcc agataccaca atccatacgg
    18601 tatgaacatt tcctcgcctc aatgttgtag tgtagaatgc ggcgtttcta ttaatacaga
    18661 cgttaagctc agaacagcga cttctaaaag cctggttaac cgggcattaa ggcacaataa
    18721 tcatacttta tgaagaatat acgtaacttt tcgatcatag ctcacattga ccacggtaaa
    18781 tcgacgctgt ctgaccgtat tatccagatc tgcggtggcc tgtctgaccg tgaaatggag
    18841 gcgcaggttc tcgattccat ggatcttgag cgtgagcgtg gcattaccat caaagcgcaa
    18901 agcgtgacgc tggactacaa agcgtctgac ggcgaaacct atcagcttaa ctttatcgac
    18961 accccgggcc acgtagactt ctcctatgaa gtttcccgtt cgctggctgc ctgtgaaggt
    19021 gcattgctgg tggtcgacgc cgggcagggc gtagaagcgc aaaccctggc aaactgctac
    19081 accgccatgg aaatggatct cgaagtggtg ccggtactga acaagattga cctgccggca
    19141 gccgatcctg aacgcgtggc ggaagaaatt gaagatatcg tcggcatcga cgccaccgat
    19201 gcagtgcgct gttcagcgaa aaccggcgtt ggcgtgcagg acgttctcga acgtctggtg
    19261 cgcgacattc cgccgccgga aggcgatccg gaaggcccgt tgcaggcact aattatcgac
    19321 tcctggttcg acaactacct gggcgttgtt tcacttatcc gtattaaaaa cggcaccctg
    19381 cgtaagggcg acaaagtgaa agtcatgagt accgggcaga cctataacgc cgaccgtctg
    19441 ggcatcttca cgccgaaaca ggttgaccgc actgaactga aatgtggcga agtaggctgg
    19501 ctcgtatgtg cgattaaaga tatccacggc gctccagtcg gcgatacctt aacgctggcg
    19561 cgtaatccgg cagaaaaggc gctgcctggc tttaagaaag tcaaaccgca ggtatacgcc
    19621 ggtctgttcc cggtaagttc cgacgactat gaagccttcc gtgacgcgct gggtaaactc
    19681 agcctgaacg atgcctcact gttctatgag ccggaaagct ccagcgcgct gggctttggt
    19741 ttccgctgcg gcttccttgg cctgctgcac atggagatca tccaggagcg tctggaacgt
    19801 gaatacgatc tggatctgat caccactgcg ccgaccgtag tgtatgaagt tgaaaccacg
    19861 tcaagggaag ttatctacgt cgacagccca tccaagctgc ctgcggtaaa taacatttac
    19921 gaactgcgcg agccgattgc agagtgtcac atgctgctgc cgcaggcata tctcggcaac
    19981 gttattacgc tgtgcgtaga aaaacgcggc gtgcagacca atatggttta ccacggtaat
    20041 caggtggcgc tgacgtacga gatcccgatg gcggaagtgg tgctcgactt cttcgatcgc
    20101 ctgaaatcta cctcgcgtgg ttatgcgtct ctggattaca acttcaaacg cttccaggcg
    20161 tccgacatgg tacgtgtaga cgtattaatc aacggtgaac gtgttgatgc gctggcgttg
    20221 atcacccacc gtgataattc gcaaaaccgc ggtcgcgagt tggtggagaa gatgaaagat
    20281 ctgatcccac gccagcagtt tgatatcgcc attcaggcag cgattggtac gcacattatt
    20341 gcgcgatcca ccgtgaaaca gctgcgtaaa aacgtactgg ctaaatgtta tggcggcgat
    20401 atcagccgta agaaaaagct gctgcagaag cagaaagaag gtaagaaacg catgaagcag
    20461 atcggtaacg ttgagctgcc gcaggaagcg ttcctcgcca ttctgcacgt cggcaaagac
    20521 aacaaataac ccttaggagt tggcatggcg aatatgtttg ccctgattct ggtgattgcc
    20581 acactggtga cgggcatttt atggtgcgtg gataaattct ttttcgcacc taaacggcgg
    20641 gaacgtcagg cagcggcgca ggcggctgcc ggtgactcac tggataaagc aacgttgaaa
    20701 aaggttgcac cgaagcctgg ctggctggaa accggagctt ctgtttttcc ggtgctggct
    20761 atcgtattga ttgtacgttc gtttatttat gaaccgttcc agatcccgtc aggttcgatg
    20821 atgccgactc tgttaatcgg tgattttatt ctggtagaga agtttgctta tggcattaaa
    20881 gatcctatct accagaaaac gctgatcgaa accggtcatc cgaaacgcgg cgatatcgtg
    20941 gtctttaaat atccggaaga tccaaagctt gattacatca agcgcgcggt gggtttaccg
    21001 ggcgataaag tcacttacga tccggtctca aaagagctga cgattcaacc gggatgcagt
    21061 tccggccagg cgtgtgaaaa cgcgctgccg gtcacctact caaacgtgga accgagcgat
    21121 ttcgttcaga ccttctcacg ccgtaatggt ggggaagcga ccagcggatt ctttgaagtg
    21181 ccgaaaaacg aaaccaaaga aaatggaatt cgtctttccg agcgtaaaga gacactgggt
    21241 gatgtgacgc accgaattct gacagtgccg attgcgcagg accaggtggg gatgtattac
    21301 cagcagccag ggcaacaact ggcaacctgg attgttccgc cgggacaata cttcatgatg
    21361 ggcgacaacc gcgacaacag cgcggacagc cgttactggg gctttgtgcc tgaagcgaat
    21421 ctggtcggtc gggccacggc tatctggatg agcttcgata agcaagaagg cgaatggccg
    21481 actggtgtgc gcttaagtcg cattggcggc atccattaat agccatcttc gttcacgttg
    21541 tcgccgttat ggcgacaacg tgaattattt atgagataaa tctcccgtgg ctaacgacat
    21601 cccccgtcgt tgtgtataga atattccccc gaagtttaag gttggcacct tcaggttgcc
    21661 acggcacacg aaacagcgtt ggtccccata taccgctaaa ctgaaactgc agcgaagcag
    21721 ttagcagagc catgtatatc aggtctgttt cgtgtgctga attgttgacg catttattta
    21781 ttggtatcgc atgaacccca tcgtaattaa tcggcttcaa cggaagctgg gctacacttt
    21841 taatcatcag gaactgttgc agcaggcatt aactcatcgt agtgccagca gcaaacataa
    21901 cgagcgttta gaatttttag gcgactctat tctgagctac gttatcgcca atgcgcttta
    21961 tcaccgtttc cctcgtgtgg atgaaggcga tatgagccgg atgcgcgcca cgctggtccg
    22021 tggcaatacg ctggcggaac tggcgcgcga atttgagtta ggcgagtgct tacgtttagg
    22081 gccaggtgaa cttaaaagcg gtggatttcg tcgtgagtca attctcgccg acaccgtcga
    22141 agcattaatt ggtggcgtat tcctcgacag tgatattcaa accgtcgaga aattaatcct
    22201 taactggtat caaactcgtc tggacgaaat tagcccaggc gataaacaaa aagatccgaa
    22261 aacgcgcttg caagaatatt tgcagggtcg ccatctgccg ctgccgactt atctggtagt
    22321 ccaggtacgt ggcgaagcgc acgatcagga atttactatc cactgccagg tcagcggcct
    22381 gagtgaaccg gtggttggca caggttcaag ccgtcgtaag gctgagcagg ctgccgccga
    22441 acaggcgttg aaaaaactgg agctggaatg agcatcgata aaagttactg cggatttatt
    22501 gccatcgtcg gacgtccgaa cgttggcaaa tccacattgt tgaacaaact gctggggcag
    22561 aaaatctcca tcacctcccg caaggcgcag acaactcgtc accgcattgt ggggatccat
    22621 actgaaggcg cgtatcaggc gatctacgtc gatacaccgg gcctgcatat ggaagaaaaa
    22681 cgcgccatta accgcctgat gaacaaagcg gcgagcagct ctattggcga tgttgagctg
    22741 gtgatttttg tcgttgaagg cacccgctgg acgccggacg acgaaatggt gctcaacaaa
    22801 ctgcgcgaag gcaaagcgcc ggtaatcctc gcggtgaaca aagtggacaa cgtgcaggag
    22861 aaagccgatc tgctgccgca cctgcagttc ctggcaagcc agatgaactt cctcgatatc
    22921 gtgccaatct ctgccgaaac cgggctgaat gttgacacta ttgcggcaat cgtgcgtaag
    22981 catctgcctg aagcgactca tcacttcccg gaagattaca tcaccgatcg ctcacagcgt
    23041 tttatggcgt ctgaaatcat ccgcgaaaaa ctgatgcgtt tccttggcgc tgaactgccg
    23101 tactccgtga ccgtggagat cgaacgtttc gtctctaacg aacgcggcgg ttgtgacatc
    23161 aacggtttga ttctcgttga gcgtgaaggg cagaagaaga tggttattgg caacaaaggg
    23221 gccaagatca aaaccatcgg gattgaagcg cgtaaagaca tgcaggaaat gttcgaagcg
    23281 cctgtccacc ttgaactgtg ggtaaaagtg aaatcgggtt gggccgacga cgaacgcgca
    23341 ctgcgcagtc tcggttatgt tgacgatctt taagagtcac tccgatggaa ggctggcagc
    23401 gcgcatttgt cctgcatagt cgcccgtgga gcgaaaccag cctgatgctg gacgtcttca
    23461 cagaggaatc gggtcgcgtg cgtctggttg ccaaaggcgc acgctctaaa cgctctactc
    23521 tgaaaggtgc attacagcct ttcacccctc tcttgctacg ttttggcggg cgtggcgaag
    23581 tcaaaacgct gcgcagtgct gaagctgtct cactggcgct gccattaagc ggtatcacgc
    23641 tttacagcgg tctgtacatc aacgaacttc tctcccgcgt actggaatac gagacgcgct
    23701 tctctgaact ctttttcgat tacttgcact gcattcagtc tcttgcaggt gacactggta
    23761 cgccagaacc cgcgctgcgt cgctttgaac tggcactact cgggcatctg ggctatggcg
    23821 tcaattttac ccattgcgcg ggtagcggcg agccggtaga tggcaccatg acgtatcgtt
    23881 atcgcgaaga aaaagggttt atcgcaagcg tcgttatcga caataaaacg ttcaccggaa
    23941 ggcagttaaa agcgttaaac gcgcgggaat ttcctgacgc agacacacta cgcgccgcga
    24001 aacgctttac ccgaatggcg cttaagccgt atcttggcgg taaaccatta aagagcaggg
    24061 aactgttccg gcagtttatg cctaagcgaa cggtgaaaac acattatgaa tgatgaggat
    24121 tgtcatggct gaattactgt taggcgtcaa cattgaccat attgcaacgc tgcgtaacgc
    24181 acgcggtact gcttacccgg atccggtgca ggcggcgttt attgccgagc aggcgggagc
    24241 ggacggcatt accgtgcatt tacgtgaaga tcgccgccac attactgacc gcgatgtgcg
    24301 catcctgcgg cagacgctgg atacccgaat gaatctggag atggcagtga ccgaagagat
    24361 gctggcgatc gccgttgaga cgaagccaca tttttgctgc ttggtaccgg aaaagcgtca
    24421 ggaagtaaca accgaaggcg gcctggacgt tgcagggcag cgtgaaaaaa tacgcgatgc
    24481 ctgcaaacgt ctggcagatg ccgggattca ggtttctctg tttatcgacg ccgatgaaga
    24541 gcagatcaaa gctgcggcag aggttggcgc accgtttatc gagatccaca ccggttgcta
    24601 tgctgatgcc aaaactgacg ccgaacaggc gcaagagctg gtgcgtatcg ccaaagccgc
    24661 gacctttgcc gctagtctcg gtctgaaagt taacgccgga cacggtctga cctatcacaa
    24721 cgtgaaagcc attgccgcca tccctgagat gcatgaactg aatatcggtc atgccattat
    24781 tggccgtgca gtgatgaccg gactgaaaga tgcggtggca gaaatgaagc gtctgatgct
    24841 ggaagcgcgt ggctaatggc aatattaggt ttaggcacgg atatcgtaga gatcgctcgc
    24901 atagaagcag tgatcgcccg atccggtgag cgcctggcac gccgcgtatt aagcgataac
    24961 gaatgggcaa tctggaaaac gcaccaccag ccggtgcgtt ttctggcgaa gcgttttgct
    25021 gtgaaagaag ccgcagcaaa agcgtttggc accgggatcc gcaatggtct ggcgtttaat
    25081 caatttgaag tattcaatga tgagctcggc aaaccacggc tacggctatg gggtgaggca
    25141 ttaaaactgg cggaaaagct cggcgttgta aatatgcatg taacgctggc agatgaacgc
    25201 cactatgctt gtgccacggt aattattgaa agttaaaata ataccgttct ctttaaaaag
    25261 taaaaccccc gaactggtaa ggaacggggg ttttactttt taactttaat tttgtgcagc
    25321 atgcaccctc aacaacagcg attatggatc agtgtactta tcttttacaa gcaacttgca
    25381 atctttagca taaaaactcg agcctttacg aagaaagcaa tattgatgga aagattaacg
    25441 tgaccgccaa ttcgtaagta cattaaaatt ggcttcgtta ttgaagattt tgctgtgctt
    25501 tacaccatgc cacagaattc ccccattgaa acgagtggtg tcgtcaaagc tctggtgtgg
    25561 agtgcagcat gcaccctcaa taactcgcac gttcagtttt ggggagatgt aagggctaat
    25621 ctgaatggct gcattccttg tttaaggaaa aacgaatgac tgattgccga tacctgatta
    25681 aacgggtcat caaaatcatc attgctgttt tacagctgat ccttctgttc ttataacaca
    25741 aggaaacgta cttaaggtgc gtccggtgaa ccaatcggac gcgcctttaa taactataaa
    25801 taagtgtctt ggcagatact atataaatta acttagtgaa tgattatgct aatgtcatca
    25861 attaaataaa tataatggcg ttaaggcttc ccagtaatat aattaatact ctacttccag
    25921 agtagaatat taaattttat ccgcgtggtg catcagcaca aatttatccc acaactgttc
    25981 ttctgtctcg acatgcgccg gatctttcac aatagtattg gggatcgggc acaccttctg
    26041 gcaggttggt gtctcgtagt gccctacgca ttcggtacac ttatcgctgt taatttcgta
    26101 gatatgatct cccattgaaa tcgcctcatt cgggcattcg ggttcacaca tatcacaatt
    26161 aatacagcgt ttagtaatta gtaaagacat ttcaatggat taccgttaaa tcattttaaa
    26221 atcagtaagt tgtatcgagt ttgtatgctt tactgtcatt aacttactgt atgttgatcc
    26281 agtgtattta accttgataa actcagtcca gcaacacaaa accgcaacac attgcatttt
    26341 gtcccgtaga aaagacttgt atgtgtgagc ttgttttctg cgcctacgca gataaggatt
    26401 gagaatgccg cgcactgtaa cacataatcc ggatagcccc aataatgacg atgttttagc
    26461 cgcatctgaa aaatgggacg cctgtaaacc cccctatacc agcgcacaca tgaaaatctg
    26521 tgttgctgcc gccaaaatca tcctcgctgc ttccggcgtg gctcgctgtt ccaaatacga
    26581 aaaagagaac tatctccgta tcgatttcag caaagccggt aaggttacat tttacgccga
    26641 gtttccaaaa aagatgggcc tcaaaggtaa aaagctcggc gagtggccgg agctcgttat
    26701 ccagctggcg cgcgaaaaag cactaggtat ggctgacggt ggactgcggg cagagtccgt
    26761 acatgcagcg ctggaaatgt accgggatga cccaaagcca aagtagcccg gcagaagctg
    26821 agcccggaca gtttcacaac ctacggggtg cgtatcgacc ggattaaagt aacgttcggc
    26881 gagcgtgagg tgttcagcga cgtaacatac aatcggctgg tggaagtgct ggacgagtgg
    26941 atcgccactc gctcgaacaa taacgccctg gagttgtttg ccgagctccg tcggttctgg
    27001 aagttctgcg cacctactct ttgcaacggc cgcaatgttg ccgccagtct gccagatgat
    27061 tatgtttcct cccgcgtaca gaaacctacc cccacacggc tttttaccga tattgaatca
    27121 atcgcccgac tctggctcaa tgttgctgcc tgcacctctg tacaccagaa gaatgctgtt
    27181 cgcttcatga tcatcactgg tgttcgtccg attaatgtcc ataacctgcg ctgggactac
    27241 gtttacgagg aggctggtga aattgtttat ccggaagggg ttatcggcat gcgaggggct
    27301 atgaaaacac aaaaggcttt ccgcctgccg ataacgcctg agatccggcg gattatcgac
    27361 gagcagaaag cctggcgtga ttcagttcct gagtgcaaca gggattatgt atttttgcag
    27421 ccacgtgatc caatgcagcc attttcaaaa cgatcactgg ataagccggt gaaaacatac
    27481 agcccggacg gggctgtaaa atgaggtgta ctggcaatag cggacactac catttgttct
    27541 ttttttaagc agccatctga tgat
//
LOCUS       DAJTSA010000052        26563 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000052 DAJTSA010000000
VERSION     DAJTSA010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.052"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            348..641
                     /locus_tag="OO369_002327"
     CDS             348..641
                     /locus_tag="OO369_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR5982582.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            713..1372
                     /locus_tag="OO369_002328"
     CDS             713..1372
                     /locus_tag="OO369_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR5982583.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1464..1910
                     /locus_tag="OO369_002329"
     CDS             1464..1910
                     /locus_tag="OO369_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR5982584.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="OO369_002330"
                     /pseudo
     CDS             complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="OO369_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3390..3809
                     /locus_tag="OO369_002331"
     CDS             3390..3809
                     /locus_tag="OO369_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR5982585.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSTITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3809..5077
                     /gene="umuC"
                     /locus_tag="OO369_002332"
     CDS             3809..5077
                     /gene="umuC"
                     /locus_tag="OO369_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR5982586.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="OO369_002333"
     CDS             complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="OO369_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR5982587.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="OO369_002334"
     CDS             complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="OO369_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR5982588.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7561..8280
                     /gene="fadR"
                     /locus_tag="OO369_002335"
     CDS             7561..8280
                     /gene="fadR"
                     /locus_tag="OO369_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR5982589.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8332..9864)
                     /locus_tag="OO369_002336"
     CDS             complement(8332..9864)
                     /locus_tag="OO369_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR5982590.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10194..11492
                     /gene="dadA"
                     /locus_tag="OO369_002337"
     CDS             10194..11492
                     /gene="dadA"
                     /locus_tag="OO369_002337"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR5982591.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11502..12572
                     /gene="dadX"
                     /locus_tag="OO369_002338"
     CDS             11502..12572
                     /gene="dadX"
                     /locus_tag="OO369_002338"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR5982592.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12740..14154)
                     /locus_tag="OO369_002339"
                     /pseudo
     CDS             complement(12740..14154)
                     /locus_tag="OO369_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14765..16501)
                     /locus_tag="OO369_002340"
     CDS             complement(14765..16501)
                     /locus_tag="OO369_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR5982593.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16596..17510)
                     /gene="ldcA"
                     /locus_tag="OO369_002341"
     CDS             complement(16596..17510)
                     /gene="ldcA"
                     /locus_tag="OO369_002341"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR5982594.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17610..18221
                     /gene="emtA"
                     /locus_tag="OO369_002342"
     CDS             17610..18221
                     /gene="emtA"
                     /locus_tag="OO369_002342"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR5982595.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18223..18957)
                     /locus_tag="OO369_002343"
     CDS             complement(18223..18957)
                     /locus_tag="OO369_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR5982596.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19158..19412
                     /locus_tag="OO369_002344"
     CDS             19158..19412
                     /locus_tag="OO369_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR5982597.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19462..20922)
                     /locus_tag="OO369_002345"
     CDS             complement(19462..20922)
                     /locus_tag="OO369_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR5982598.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20859..21416)
                     /locus_tag="OO369_002346"
     CDS             complement(20859..21416)
                     /locus_tag="OO369_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCR5982599.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21442..22296)
                     /locus_tag="OO369_002347"
     CDS             complement(21442..22296)
                     /locus_tag="OO369_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCR5982600.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22296..23105)
                     /locus_tag="OO369_002348"
     CDS             complement(22296..23105)
                     /locus_tag="OO369_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR5982601.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     TLKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23115..23873)
                     /locus_tag="OO369_002349"
     CDS             complement(23115..23873)
                     /locus_tag="OO369_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5982602.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23870..24850)
                     /locus_tag="OO369_002350"
     CDS             complement(23870..24850)
                     /locus_tag="OO369_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR5982603.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24850..25872)
                     /locus_tag="OO369_002351"
     CDS             complement(24850..25872)
                     /locus_tag="OO369_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCR5982604.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            26222..>26563
                     /locus_tag="OO369_002352"
     CDS             26222..>26563
                     /locus_tag="OO369_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5982605.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTGCSNDNIL"
BASE COUNT         6675 a         6469 c         6854 g         6565 t
ORIGIN      
        1 tatggccttt gctgaactca acattgacat tcttacccgc agcaaagctc gtggaggtta
       61 gcagtgcaag cagcaataca gccttactga tgctcttgat tttcattggt agtctcctgt
      121 tcagaatgag tacgatgaac tgttataata taacaatccc ttacgggaga attccagtgg
      181 cttttctgcg ttcggtaaaa tcccggcgcg gcattttttg ccggggggat tacttataaa
      241 ggggcatgga ctcaacaaac cgcagaaatc tacagctggc tggcttgtgg cgcgggtttc
      301 attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg ttttgtgcga
      361 tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa gacgattttt
      421 cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg atgattctgc
      481 cgttggatgg gcggaagaaa ctggtcaatg ctgatattga aaaggttaaa ctggcattaa
      541 ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg aagcaacatc
      601 tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc ggcggtggca
      661 ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg ttatgtatca
      721 acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag tctgtgttgg
      781 cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag agccagtgct
      841 gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga tcccatccga
      901 tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggcgcga cgctgcgtca
      961 ggcaacggaa gagcatgtcc gcaaagccat tgccggttac ggcgtggcgc ttgatctgac
     1021 attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc
     1081 gtttgataac tcttgtccgc tttccgggtt tatccccgcg gcggaattta ccggcgatcc
     1141 acaaaataca acgctgggcc tgagcgtaaa cggcgaaaaa cgccagcaag gtacgactgc
     1201 ggacatgatc cataaaatcg ttccgttgat cgcttatatg agcaagtttt ttaccctcaa
     1261 ggccggtgac gttgtgttga caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga
     1321 tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt aatacttttt
     1381 gccgccagaa agcggcggca aaacttgcat cgctgtgcca gactggttat aaggtgcgtt
     1441 ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt aaaaccctgg
     1501 acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcttgc
     1561 ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc tgtcgccagc
     1621 tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt gaacccgact
     1681 gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg acctgcgctt
     1741 atcggttgct ggcggaaggt aaagatttac ctgcgtggca tccgctactt actggttcga
     1801 aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag
     1861 tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga taaatttaat
     1921 agatgctgat gggggctttc ttaatatacg tctggatgct cgccatttcg tagccctgtt
     1981 tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac aggtaactct
     2041 caatctttaa aaaacgtctg tgtttttaaa tgtgataaac cttatacctt gagtacatgg
     2101 agagaataat cgcttatcag acttcaggta cttcaaagag tgtcttttta ccgtgtcttt
     2161 tctgatactc attacaggtg ttaatcattt ttttggccgc tgcttttagc aaagaaagca
     2221 ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt ttccgttttt atctccccga
     2281 tggcggctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta ttcgcttgtt
     2341 taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaacttg ttcttcaatt
     2401 ctggaatcag tttcccttca actacgcccg cagcaataga ataggaaatg attaaaccaa
     2461 atggaccggc gacgacatcg gctgcggcac cggcatacgc ttccttcctg attttatcta
     2521 cctgtgactg gaaatagctg cttttttctg aaaaatcatt ggttaactgg ctatctaacg
     2581 ccagcagttt cccggaagcg ttgttgaaac tttgtgagct taccagcagg gatttttgcg
     2641 cttcattcag cttcgtgatg ccgtcatcca gtaccttaat gaggggcgga tgctttcttc
     2701 tcattgtact catcaaatag caaaatatac gctgcgagca attgcgtcgc aacaccacac
     2761 tattcataca ctgtttgggt tgcttcaaaa tacttatcct ggctatccat aagtaaggtt
     2821 ttaatatcgc cgactaaaac ggaggctgcc tgtgaatact cctgtttaaa gcgacttaac
     2881 tcttttatgg tttcatcaaa ggtctgccag gggatgacct gatcgagata tttattataa
     2941 agatctaatg ctccatctgc ggtttcgatt gcgtttttaa ctacttctac cgttttatct
     3001 gcaacgattt cagtcataat cattcgcctc tttaaatata taaattgtaa tgaaactcct
     3061 gttttacaac tattaataaa ttttacttca tctaattcat agttagccgg gcgggatgcg
     3121 tcaatgtctt tatttctatt aatatgataa atatcaaaca atgtttaatg tcattatggc
     3181 gaatgcttct attctatttt tttgccgggt gatatttttc atttctgctg gatgagcgtc
     3241 gtcgccagaa ggccacgtga gcacaggata agagaacgaa aaatcagcag tctatgcagc
     3301 gacaaatatt gatagcctga atcagtattg atctgctggc aagaacagac cactgtatat
     3361 aaaaacagta taacttcagg cagattatta tgttgtttat caagcctgcg gatctccgcg
     3421 aaattgtgac ttttccgcta tttagcgatc ttgttcagtg tggctttcct tcaccggcag
     3481 cagattacgt tgaacagcgc atcgatctga atcaactgtt gatccagcat cccagcgcga
     3541 cttacttcgt caaagcaagt ggtgattcta tgattgatgg tggaattagt gacggtgatt
     3601 tactgattgt cgatagtact attaccgcca gccatggtga tattgtcatc gctgctgttg
     3661 acggcgagtt tacggtgaaa aaattgcaac tacgcccgac ggtacagctt attcccatga
     3721 acagtgcgta ctcgcccatt accatcagta gcgaagatac gctggatgtc tttggtgtgg
     3781 tgatccacgt cgttaaggcg atgcgctgat gtttgccctc tgtgatgtaa acgcgtttta
     3841 tgccagctgt gagacggtgt ttcgccctga tttatggggt aaaccggtgg ttgtgctatc
     3901 gaataatgac ggttgcgtta tcgcccgaaa cgctgaggca aaggcgctgg gcgttaaaat
     3961 gggcgatccc tggttcaaac aaaaagatct gtttcgtcgc tgtggtgtgg tttgctttag
     4021 cagcaattat gagctttacg cagacatgag caatcgggtg atgtcgacgc tggaagaact
     4081 atcgccccgc gtcgagattt acagtattga tgaagcattc tgcgatctga caggtgtgcg
     4141 taattgtcgc gatctgactg attttggcag agaaattcgc gcaacggtgc tacaacgtac
     4201 ccatcttact gttggtgtgg ggatcgccca gaccaaaacg ttggctaagc ttgccaatca
     4261 tgcggcaaaa aaatggcagc ggcagacggg tggggtggtg gatttatcaa atctggaacg
     4321 ccagcgtaaa ttaatgtctg ctctccccgt ggatgacgtc tgggggattg gacggcggat
     4381 cagcaaaaaa ctggacgcga tggggatcaa aaccgttctc gatttggcgg atacagatat
     4441 ccggtttatc cgtaaacatt ttaatgtcat gctcgaaaga acggtgcgtg aactgcgcgg
     4501 cgaaccctgt ttgcaactgg aagagtttgc accgacgaag caggaaatta tctgttcccg
     4561 ctcgtttggt gaacgcatca cggattatac gtcgatgcgg caggccattt gtagttacgc
     4621 tgcccgggcg gcggaaaaac ttcgcagtga gcatcaatat tgtcggttta tctccacatt
     4681 tattaagacg tcaccatttg cgctcaatga accttattac ggcaatagcg cgtcggtaaa
     4741 actgctgacg cccactcagg acagcaggga tatcattaac gctgctacgc gatctctgga
     4801 tgccatctgg caagcgggcc atcgctacca aaaagcgggc gtgatgctgg gggatttctt
     4861 cagtcaggga gtcgcgcagc tcaatttatt cgatgacaac gcaccgcgcc ccgggagtga
     4921 gcaattgatg gcggtaatgg atactctgaa tgctaaagag ggcagaggaa cactctattt
     4981 tgccgggcag gggatccaac aacaatggca gatgaagcga gctatgcttt caccacgtta
     5041 tacaacgcga agttctgatt tactgcgggt caaataaata tagcggcagg aaaaaagcgc
     5101 tcccgcagga gcgccgaatg gattagcgac cgaacagatc acgttttttc gctttaaacg
     5161 gctgggaaat caccaccagc accgcgacaa tcaggtaagc gataaaaata ccgagcagcc
     5221 actgcggcat ttccatacct aaaaaatccc actgacgctc ggcgcaatcg ccagaggcga
     5281 caaacacttg cggcacccat ttatccagcg gcagccattc cgggaaacga accataaaat
     5341 cacaggtggc aaatggcgaa ggatagagct gaagcatggt gtgctcgtaa gttaactgca
     5401 caccgcggaa cgcactatac aaccagataa ccatcgctac ataacgcagc ggagttttcg
     5461 gggcgatcgc gccaatcagc gcagcaccca gaacgccgaa taacgcgcag cgttcataaa
     5521 tacagagcac gcaaggtttc agtaacatca catgctggaa ccacagcgcc gtcagttcca
     5581 gtgccagagc agtaaacgcc atcaacagcc acgcgccccg gccttgtgaa cattggttca
     5641 aaaatcgcaa cataatcatt tccctgcaat atgcatagag tgcgcagttt aaaccaattc
     5701 attcgctgcg ccaccaggag gcagcaaaaa taggacgcaa ttggacaatt atcgggcgaa
     5761 aaggcaaacc gggcatttat atgcccggta agttgttatc aaagcgttgc tatccagccc
     5821 atttgcataa accattcggt tacaggggca agcgtaaact cgacgcagag caagccgacg
     5881 agtgtcagta cgagggtgta aggcagggcc atccacacca tgcggccata agagaggcga
     5941 atcaatggcg cgagtgcaga ggtcagcagg aacaggaacg cagcctgacc gttcggcgta
     6001 gcgacagagg gcagattggt accggtatta atggcgaccg ccagcagctc gtattgcttc
     6061 aacgtgatag cgccactttc cattgccgct ttcgcttcgt tgatataaat cgtccccacg
     6121 aagacgttat ccgaaatgga tgacagcaga ccgttgaaaa tatagaacag cgacagctga
     6181 gcatgttcag atgcctgcaa cacaaactga ataatcggcg aaaacagttg ttggtcgata
     6241 atcaccgcga ctaccgagaa aaacaccgtc aacagtgcgg tgaatggcag agattcggtg
     6301 aaggctttac cgatagcatg ttcatcggtg acaccggtca atgatgttgc cagaataatg
     6361 actgacaaac caatcaagcc aacttccgcc agatgcaacg ccagcgcagt caccagccag
     6421 acgccaataa tcgcctggac aatcagacgg attttatcct gacgggtacg ctggtggcgg
     6481 ctttgatcgt caaactgttg caacacttcg cggactttct ccggcagcgt ttcaccgtag
     6541 ccaaaccaac gcagcttctc taccagcagg caggttaaca ggccacaaat cagaaccgga
     6601 acggtcaccg gcgacatgcg caggaagaaa tcgccaaaat gccagccagc cgctttagcg
     6661 atgatcaggt tctgtggttc gcccaccatg gtcattacgc cgcctaatgc ggtgccgaca
     6721 ccggcatgca tcatcaggct acgcagaaag ccacggaact gttccagaac cacgttgtaa
     6781 tgcttgtcga tatgactatc gtcttgcagg tcggtgtctt cggtacggga agaggctacg
     6841 cgatgataaa taccataaaa accgactgca acgctgatca ccaccgccac gacggttaag
     6901 gcatcgagga acgcggagag gaacgctgcc gccacgcaaa aagagagcga cagcagcatt
     6961 ttggagcgaa tgcttaacag caaacgggta aatatgaaca gcaacagctg tttcataaaa
     7021 tagatacccg ccaccataaa catcaataac agcaagactt caagatttgc cgccacctct
     7081 tcacggacgt gttccgcgct ggtcatgccg atgaataccg cttcgatagc caacagacca
     7141 ccggggagta gcgggtagca tttcagggcc atcgccagag tgaaaataaa ttccgctacc
     7201 agcaaccagc ccgcgacgaa agggctgatg aggaaaatta acgggtttac gattaagaaa
     7261 atgatgaggg cgagtttgta ccagtcgggg gactggccca aaaaattgcg ccatagcgcg
     7321 cggccccagg agatctccat gatggtttcc cttaccttac aaataatcaa tgatgttttt
     7381 atgtttaaac gcaaagctta acggtcaggc aggagtgagg caagtcttga tagtcaaggg
     7441 gaaagagatg cggaaaatga agccttgatc cctttttctt ctttttgtct gctatcagcg
     7501 tagttagccc tctggtatga tgagtccaac tttgttttgc tgtgttatgg aaatctcact
     7561 atggtcatta aggcgcaaag cccggcgggt ttcgcggaag agtacattat tgaaagtatc
     7621 tggaataacc gcttccctcc cgggactatt ttgcccgcag aacgtgaact ttcagaatta
     7681 attggcgtaa cgcgtactac gttacgtgaa gtgttacagc gtctggcacg agatggctgg
     7741 ttgaccattc aacatggcaa gccgacgaag gtgaataatt tctgggaaac ttccggttta
     7801 aatatccttg aaacactggc gcgactggat cacgaaagtg tgccgcagct tattgataat
     7861 ttgctgtcgg tgcgtaccaa tatttccact atttttattc gcaccgcgtt tcgtcagcat
     7921 cccgataaag cgcaggaagt gctggctacc gctaatgaag tggccgatca cgccgatgcc
     7981 tttgccgagc tggattacaa catattccgc ggcctggcgt ttgcttccgg caacccgatt
     8041 tacggtctga tccttaacgg gatgaaaggg ctgtatacgc gcattggtcg tcactatttc
     8101 gccaatccgg aagcgcgcag tctggcgctg ggcttctacc acaaactgtc ggcgttgtgc
     8161 agtgaaggcg cgcacgatca ggtgtacgaa acagtgcgtc gctatgggca tgagagtggc
     8221 gagatttggc accggatgca gaaaaatctg ccgggtgatt tagccattca ggggcgataa
     8281 tcccttccgt ttaaagagca accccctcaa acgaggggtt ttttgttgtt tttacagatt
     8341 tcccattctt ggcgggcaac gttccagcaa ctcgatgctg ccgtcttcgt tttgctgttc
     8401 gagcatcaca tcaaatcccc acaggcgatg cacatgcttc aggacttctt tgcgcccccg
     8461 atccagcggt gcgcgattat gtggaatata acgcagcgtc agcgaacggt cgccgcgcaa
     8521 atccacgttc cagatctgaa tattcggctc cagattactt aagttatatt gcgacgataa
     8581 ccggttacgg atctcccgat aaccttcttc attatgaata gcggaaatct ccagataatt
     8641 atgccgatca tcatccagca cggtgaagaa gcggaaatca cgcatcactt tcggtgacag
     8701 aaactggctg ataaaactct catctttgaa atcacgcatc gcgaaatgca gcgtttccag
     8761 ccagtcggaa ccggcgatat ccgggaacca gtatttgtct tcttccgttg gcgactgaca
     8821 aatccgttta atatcctgga acatggcgaa cccgagggca tacgggttga tgccgctgta
     8881 ccacgggctg ttataggggg gctggaagac cacattggtg tggctgtgca aaaactccag
     8941 cataaaacgt tccgttactt tcccttcatc atacagatgg ttaaggatgg tatagtgcca
     9001 gaaggtcgca cagccttcgt tcatcacctg agtctgtttt tgcggataaa aatactggct
     9061 caccttacgc acaatacgca ggatttcacg ctgccatgat tccagcagtg gcgcattttt
     9121 ctccataaaa tagagcaggt tttcttgtgg ttcggacgga tagcggcgcg cttcagcaac
     9181 cgttttctct tcctcgcgct tcggcagggt acgccagagc atattgacct gactttgtag
     9241 atactcttcg cgacttttct gccgggcttt ctcttcttgc agcgagattt tttgcgggcg
     9301 tttgtaccgg tccacgccgt agttcatcag cgcgtggcac gagtccagaa gccgttctac
     9361 ttcatcaacg ccataacgtt cttcgcactc ggtaatatat ttacgggcaa aaatcagata
     9421 atcgacaatc gaactggcgt cggtccaact gcggaataag caattgtttt taaagaaaga
     9481 gttatgtcca tagcaggcgt gtgccatcac cagcgcctgc atagtaatgg tgttctcttc
     9541 catcaagtaa gcgatacacg ggttagagtt aatgacgatt tcataggcca gcccttgctg
     9601 accgtgctta tacagccgtt cagtctcgat aaacttttta ccgaatgacc agtgcggata
     9661 gttaattggc atgccgacgc tggaataggc atccatcatc tgttctgagg ttatcacttc
     9721 aatctggtgc gggtaggtat ccagccggta gagtttcgcc acccggtcta tctctgccag
     9781 ataaacatcc agcaggtcga acgtccagtc gggtccatcg ctcaaacgtg tggtgtcctt
     9841 attcatagaa tcgatcgtcg ccatacgcgc acctcattgt tgtcggcgct ctctgtgtgg
     9901 agcacctcat ttcaagcata gaacacctgt taaaaaccgc gtcgccggag aatttttttc
     9961 tttgcgattt cttattatca gagtgccact aatccgcttc tgaacggaat tttatgctgg
    10021 ataaaaaggg cgttcagcag gagatactaa agacgccata ttgccgcaga gtcagggaga
    10081 tgtgagtcag ctcaccataa aaaagccgta tgttgaataa tattttcaac tgagttatca
    10141 agatgtaatt agattattat tcttttactg tatctaccgt tatcggagtg gctatgcgag
    10201 ttgtcatact gggaagtggt gtggtaggcg ttgccagcgc ctggtactta aatcaggcag
    10261 gacatgaggt caccgtcatt gatcgggagc cgggggccgc cctggaaacc agtgccgcga
    10321 atgccgggca aatctccccc ggatatgctg caccgtgggc agcaccaggt gtgcctttaa
    10381 aagcgattaa atggatgttc cagcgccatg cgccgctggc ggttcgtctc gacggtacgc
    10441 agtttcagtt gaaatggatg tggcaaatgt tacgtaactg cgacaccagc cactacatgg
    10501 aaaacaaagg gcggatggtg cgtctggcgg aatacagccg tgattgcctg aaagcattgc
    10561 gcgccgaaac caatattcag tatgaagggc gtcagggtgg gacgctgcaa ctgttccgta
    10621 ccgaacaaca gtatgaaaat gcgacccgcg atatcgccgt gctggaagat gccggcgtac
    10681 cgtatcagct gctggaatcc agccgcctgg cggaagtgga tcccgcgctg gcagaagtag
    10741 cgcacaaact gacgggcggc ctgcagttac ccaatgatga aaccggagac tgccagctgt
    10801 ttacccagaa tctggcgcgg atggcagagc aggcgggggt taaattccgc tttaatacgc
    10861 ccgttgacca actgctttgc gacggcgagc aaatctacgg cgtgaagttt ggcgatgaag
    10921 tgattaaggc cgatgcgtat gtgatggcgt ttggttctta ctcgacggcg atgctcaaag
    10981 gcattgttga tattccggtg tatccgctga aaggctactc gctgactatt ccaattgcgc
    11041 aggaagatgg tgcgccagta tccaccattc ttgatgaaac ctacaaaatc gccattaccc
    11101 gtttcgataa ccgcattcgt gttggcggaa tggcggaaat tgttggtttt aacaccgagc
    11161 tgttgcaacc gcgtcgtgaa acgctggaaa tggtggttcg cgatctctat ccacgcggcg
    11221 gtcatgtcga gcaggcgact ttctggacgg gtttgcgccc gatgacgcca gacggtacgc
    11281 cggttgtcgg gagtacacgc tttaaaaatc tgtggctgaa taccggtcac ggcacgctcg
    11341 gctggacgat ggcttgcggt tccggtcagt tgttaagcga tctgctctct ggtcgcacgc
    11401 cagcgatccc atatgaggat ctaagcgtag cgcgctacag ccgtggattt acgccatcac
    11461 gtccgggcca tttacatggc gcacacagct aaggaaacga gatgacccgt ccgatacagg
    11521 ccagcctcga tctgcaggca ttaaaacaga atctgtccat tgtccgccag gccgcgccgc
    11581 acgcgcgcgt ctggtcggtg gtaaaagcga acgcttacgg gcatggtatt gagcgtatct
    11641 ggagcgcgct cggggccacc gatggctttg cattacttaa cctggaagag gcaataacgt
    11701 tacgtgagcg cggctggaag gggccgatcc tgatgctgga aggatttttc catgctcagg
    11761 atctggagat ttatgaccag caccgcctga ccacctgcgt acacagcaac tggcagctca
    11821 aagcactgca aaatgcgcgg ctaaaagcac cgttggatat ttatcttaaa gtgaacagtg
    11881 ggatgaatcg gttgggcttc cagtccgatc gcgtgcttac cgtctggcag cagttgcggg
    11941 caatggcgaa tgttggcgaa atgaccctga tgtcgcattt tgctgaagcg gaacatcctg
    12001 atggaatttc cggcgcgatg gcgcgtattg agcaggcggc ggaggggctg gagtgtcggc
    12061 gttcgttgtc caattcggcg gcgactctgt ggcacccgga agcgcatttt gactgggttc
    12121 ggcctggcat tattttgtat ggcgcttcgc cgtccggtca gtggcgtgat atcgccaata
    12181 ccggattacg tccggtgatg acgctaagca gtgagattat tggtgtccag acgctaaaag
    12241 cgggcgagcg tgtgggctac ggcggtcgct atactgcgcg cgatgaacag cgaatcggca
    12301 ttgtcgccgc aggatacgcc gacggttatc cgcgccatgc gcctaccggt gctcctgttt
    12361 tagtggacgg cgtgcgcacc atgacggtgg ggaccgtctc gatggatatg ctggcggttg
    12421 atttaacgcc ttgcccgcag gcggggattg gtacgccggt tgagctgtgg ggcaaggaga
    12481 tcaaaattga tgatgtcgcc gccgctgccg gaacggtggg ctatgagttg atgtgcgcac
    12541 tggcgttacg cgtcccggtt gtgacagtgt aacttgttgt aagccggatc ggaggcagcg
    12601 tcttctggtt gcgaaagaat catccatccg gctggtcagc aactgtagtt gttaatgtga
    12661 cagagccatt gcccatgata gtgtccatta aaagaatgga cactatttcc ccggaacctg
    12721 aactcgccgc acaggcgttc tacataaaac gcttacgctt cattgttgac tcggagtcga
    12781 cttcagataa atcacgcttc acccttgatg cagattcggg ttcatgttca gattgttgat
    12841 taacaggctg cgagtcctgt tgtctttcca gactcaacag gcccaactgt tgatccagga
    12901 gctccaggtc ctcctgtacc ttcacgcgct ccaggagttc cgggtcatcc aggaactcca
    12961 gatccataag gtcctccagc tcctcgagtc cttccaggtc aacctagcct tccttgtgca
    13021 aaaagtccaa aaggtccttc aggccctgct gttttttcag gccctcgacg gctttgagca
    13081 gcgtcggtaa attgtctttg tcccacgggt taccattaca gtcaaacacc ttcaggctct
    13141 ggggctgcgg cacctgtacc gttttaaact tgttttctcc acagtagtaa attattagtt
    13201 tgggcggcag ccgtggtgaa gagatcaaat cgttactaga acagtcaaat actttcaggc
    13261 gagtcaagtt ctccagcatc tcatcaggta ttgagttaat attgttgccg gcacaggata
    13321 gtttatacag gtactggggc atcgacggcg gtaaaaattg caagaagttt tcagaacagt
    13381 taatcactct caggttagtc gacagctcag gcagccagat aatactgttt tcatagcact
    13441 tcagtaccat cagttttgag ggctgcacct tcggtaacaa gatcaagacg ttatctgtac
    13501 agtcaagcac cagcagccac tttggcatca ccttcggtaa cgcggtcaac acgtttttac
    13561 tgcagtaaaa ttcaatcaga tgtggaggca aattctcagg gagtgaagtc aatcccaggt
    13621 tgctaagatt gagcatactc cgtcttgtaa tgagacattc cttcattctg cgcaatgctt
    13681 ctgcccggtt ttcaatccgg cttgctggca ggttttcttc caggatattt tccttgattt
    13741 cttccagact ttctttcagg tcttcttggg gaatttctga ctgtttgttt tgctggtttt
    13801 cttccaggat ttcgtacagg tttttttgca ggttttcttg caaaccatcc tgctgtatag
    13861 cccattcgca ccatttggcg accatcatga actcgttatg ttgctgattc agacgagtcc
    13921 ccaacgtgat gatggtattg ttgtccacct gtatgttcac tttgcccgcg atactgaggt
    13981 caatgatctg ccagggggca tcaccccagc tgaatttttg cgggctgaga tacttcatgt
    14041 tctgcaaaat ttgattcttg agaagagccg ccctggcggg cgtcgtacgc ctgggttaac
    14101 ggaagatggg ggaatggcgg gggcaaatgg gcagatgtct ttaaagctag ccataatgct
    14161 acgtttcctt gaaataaata tcctctggca gcgccggaga cgtgaatgtg gtcaccgcaa
    14221 aggagccaca ggacgtcatt tgcgctgata tcactactca tcgttgactt ttttcctggt
    14281 cagaaatgcc gtgtcgggtc tggtacatgc tgtattacgg cctaggtagc tctgacgtat
    14341 taaagaaaat acgagagtga acgacccgga aataatcgtt attaagcaaa ggaataacta
    14401 taagaaatcg ctcatgcatc cggtgctaca ggcggatgaa acgcctattg tgtgggctat
    14461 gtcagtgcgg caggttgcgt gggggaatat gttcaggcga tgtcaggcag gggcccctga
    14521 tggttgaccg gtaatgcggg gctggttttt attacctgtt acagtcggaa aaagccctta
    14581 cgctgacttt cttgagccgg tgttttgagt tcattagcca gagcctgcaa tggtttgtca
    14641 tccataattt aaccttcttc tgatgttggg atgagcatat caaaatcagg aaatgacaag
    14701 tttattacag gctcaggctg cgttcagtaa agcatcgcat ccggcgatag atgctgcatt
    14761 aaatttaaga ttcagcttcc tcttcagcca cccgaacgcc aatcttcaac acttcattat
    14821 cttctttctc ggccaccgtc cagatcatcc cggcaaactc tacctggtca ccgacaaccg
    14881 gtgccgcgcc tagcaactgc tggacaattt cacccagcgt ttgctgttta tcacgatatt
    14941 ctcgcccgtc ttccaggcca tatatcagcg caacatcagc atatttggcg ctggcttcga
    15001 gaatgaagtc accaaagaag cgttgatcca gcgcgaccgg tggcgactgg ctgaacagtt
    15061 taccgagcgc cgggagatcg cgttcccgac caattacaca caatacatcg ccttcgcgca
    15121 gtcgggtgct gccggtagga tgaagcaact ggttatcacg aaacagggct gcaatacgcg
    15181 tctcttttgg catatgcaaa tcgcgcagtg ccgcacctac acaccattta tcagcactca
    15241 attgataaac aaactgctcc cacggatttt ccggatgaat atccaggcca acgcgtgaca
    15301 ccggacgtcc gacgggcgga accaccactt tggctttttt cgccgcccac gagagtgatg
    15361 ttccctgcaa gagcagtgaa accagaacca caaagaaggc gacattaaag aacagtcgcg
    15421 cattctccag ccccgccatc atcgggaaca cggctaggat gattggcacc gcgccgcgta
    15481 atcccaccca gctgataaac acgcgctcac gcagattgaa accgcggaaa gggagcaatc
    15541 cagcaaatac cgaaagggga cgggcgaaga atatcatcca tgccgacaaa atgagcgccg
    15601 gaatggcaat aggcagcaga tcgcttgggt taaccaatag ccccagcacc aggaacatgg
    15661 cgatttgcgc cagccaggcg aggccgtcga aattttgcag gatgccgtag cggttgcgaa
    15721 tcggccgatt acccagcaga aaaccgcaca gataaacagc cagaataccg ctgccttcca
    15781 gcgccgtggt taaagcaaaa attaaaatac cgccgcttaa tgccagcaat ggatataatc
    15841 cggcaggcag ggcgatgcga ttaatcattt gcagcagtaa ataaccaccg ccaagaccga
    15901 tgacaatccc gaggccaaat tgctgcagaa tatcgaccac gaacatccaa ctgacactgc
    15961 tttcatgctg ctggatcatt gcaatcaggg taatcgtcag aaagaccgcc attggatcat
    16021 tactgccgga ttcaatttcc agcgtcgagc caacacgttc gttaagcccc ttaccaccca
    16081 gcagagaaaa gaccgctgca gcatcggtgg agccgacgat agcgccgatt aataagcctt
    16141 caatcaaatc aagattaaac agccacgccg ccatcatgcc ggttaaacca gaggtgataa
    16201 gtacgcccag cgtcgccagc gacagcgccg gtcctaacgc cacacgaaag gaactggcct
    16261 gagtgcgcat cccgccgtcg agcaaaataa tcgccagcgc caggttactt accatgtagg
    16321 cgaaggggta attatcaaac gggataccgc cgacgccatc gactcccgcc agcatgccga
    16381 tcgccagaaa aataaccaga ataggaatgc caagacggga agaaaatgaa ctaagtaata
    16441 tactgctggt gacaaggatg gatcctaaga tgaaaaggct aattattgtt gtggcatcca
    16501 atggtcggtt actcctgatt acgctgtctc ttatataaac cctaccatat tagcggcaga
    16561 gacagcgttt tacttagccc tgaagcgtga tttttttaca ttttaagaac aggatgaccg
    16621 ctaatcgtca actgagcgcc ttcccgggtg ttattcagaa tggcatgtgc gcccaacggc
    16681 agcgtaaccg tgcgttgttc atgaccaaaa tcgaggccgg taatgagtgg aatcgacagg
    16741 cgggaacgca aaaacgcgta cactgactcc aggttgtaac ctgcgtcata atcattgggc
    16801 gtgctgccgc taaagctacc gagaataatc gccttctgac gtggcaaaat tcccgcatga
    16861 tagagctgca acagcatacg ttcgacgcgg aaagggtgca cgttaatatc ttccagcacc
    16921 agaataccgt tctcaatttt tggcatccac ggtgtaccaa tcagtgaaat cagcatcgca
    16981 agattgcctc cccacaacgt gccttcggtc tgacatgtcg ggccttcccc ttgccattca
    17041 atggtgaagg tttcattgcg taacgccagc cagaaatggt gctcggtaaa ggcgttcagt
    17101 tcatccgcac caaagtttgc taccagcatg gggccgctaa aggtgatgac attgccttgc
    17161 gccagaagac cgcactgaat ggcggtaaaa tcgctatgtc cgcaaatgag taacgggtca
    17221 tgttgttggc gagccaccag tgcctgccag tcaatatctg ccagtaaacg actggcaccg
    17281 taaccgccgc gtacagccag cacgatggtg ttgggggtag tcagtctggc aagggaattg
    17341 agatcttcca gacgctccgt ttccgtaccc gcaaaacgct cacaacgacg ggcaataacc
    17401 tcgacgttat tgacttgatg ccccgcgtcg gtaaggcgct ggataccacg cagcgcggcg
    17461 tgctgtttaa tgctgtaacc cgatggggca attaagtgaa acagagacat ggcaattcct
    17521 tgctgacaac agaaacgaaa tgtatatcat gccgcttagg tgtgccgttg tcacctcaac
    17581 ggcgattcca ggctataagg atagaagaag tgaaattgag atggtttgcc tttttgattg
    17641 tgttattagc gggttgttca tcaaagcatg actatacgaa cccgccgtgg aacgcgaaag
    17701 ttccggtgca acgtgcgatg cagtggatgc caataagcca gaaagccggt gcagcctggg
    17761 gcgtcgatcc acaattgatc acggcgatta tcgctatcga atcgggtggt aatccgaacg
    17821 cggtgagtaa atcgaatgcc attggtttga tgcagttaaa agcttcaacc tccggacgtg
    17881 atgtttatcg ccgtatgggc tggagtggtg agccgacgac cagcgagctg aaaaatccag
    17941 agcgtaatat ttcaatgggg gcggcttacc tgaatattct ggaaaccggc ccgctggcag
    18001 gcattgaaga tccgaaggta ctgcaatatg cgctggtggt gtcatacgct aacggggcag
    18061 gtgcgctgct acggactttc tcgtcagatc ggaaaaaggc gatcagcaaa atcaacgatt
    18121 tggatgctga cgagttcctc gatcacgtag cgcgaaatca tcctgcgccg caggctccgc
    18181 gctatatcta caaacttgag caggcactgg acgcgatgta aatcagtcgc gcactttgtc
    18241 cgctttttcc cgggcttctc gctcgagaga gaaaataatc cgctgtaatt gccgctccac
    18301 cgtcgggctg acgttaagaa aacggaagct cagacgggga gtggtgatgg tttcattctt
    18361 gccatcaatc actttgcgct cgctggtgga gattaactgg gcgtcaaagt gaaaaacacc
    18421 ccattgcccc atgttgactt caatttgagc gaagcgcata ccttcttgta attcggcagg
    18481 ctttgctgtt tccagtaatg cgcccatgcc gcctaacgac aaatcataca agcggaaacg
    18541 taacgtactg ttatccgcca gtttggtctg gcaaaaataa ggcggatgga gtggggcgga
    18601 gatgcggaaa tatcggcgtc gttgtacaaa ccataaggtg ggaggcggta cggtaataaa
    18661 tgccggaagc tgcaagtatt cactctgctg tagttgttca acagtaaact cgactttcgc
    18721 accctgagtt tcggcggtaa tggtaatgtg ctgtgccttt agcacggcga tgttgtcttc
    18781 ggcttgactg ccgaaatcca gcaccagttt atccggggtt attgccagta ttttgctgat
    18841 cagctgcccg ccattccaac tgagacgcaa aggaattgcg gctttgtgca aatcgcgtaa
    18901 cacgcccagg acggctaacg gattttgttt caggaactgc tcatggtaat gactcacgcg
    18961 gaaaaactcc tgactgacaa actgtctttg gtttatcggt catagttaac aaaacttaat
    19021 acaaatgcgt gaatattttt tacatgttgt tcttaaatca gccgacaaac gcccagcatc
    19081 gatcccgccc ttgcctatac ttagagcgtt gacgtaagca tttcttgcga ccgattcatc
    19141 gaaacgaggg cctgaacatg ggaattattg cctggattat ttttggcctg atagccggca
    19201 ttatcggcaa gctaatcatg ccggggcgtg atggtggtgg atttttcctg acctgtattc
    19261 tcgggatagt cggtgcggtg gtcggcggct ggctggcgac catgtttggc attgacggct
    19321 ccatcagcgg ttttaatttg cacagcttcc tggtggcggt ggtgggcgct atcctggtgc
    19381 tgggcgtatt ccgcctcctg cgaagagaat aagattttca taaggcggat agcgatacag
    19441 atgccgctat ccgctttcac atcagaacgt atactcgaca cctgcccaga aattgcgacc
    19501 ttcttcaata aacccttcac tgtaggcata ttgggtatca aacaggttat tgaccgacgc
    19561 attgacgctg aagccatgac ctaaggtgta atcggctcga atgtgggtca ccgcaaaacc
    19621 ggcggctttt tgtgaaccgt cactgttgct gtagctggag gaacgcgcct cttccgacag
    19681 cgttacgctt aacggctccc atggtttgag tgtcatccat gcggtcattg tctgcgttgg
    19741 aagatcggtt atcttgccga tgtctttgcg tttggcgtca gcgtggatca gagcgtagct
    19801 caatcctaca tccagtatat tgctgatttt ccccttaata ccggcatcca gaccgctgta
    19861 atccaccgtg ccgctgttct gattttgaat ggtatcggca ttgatattgt gcgagaggat
    19921 ggcatcactc acccggttat agtaaacgct gacctcaaat ccccagtcgc gcgtgaaggc
    19981 accattccag gttaaatcca ccccgcgagc gcgttccggc ttgagctgcg ggttaacaat
    20041 cgctatctgg ttatacgcag gtttggatgt ggtatagcgt tctttcagcg tcggaaagcg
    20101 tgtgcggtca tagtacgaaa gcgccagagt gtcttcattg gcaaagtggt atttccccat
    20161 cacctgccag ttaaaagctg actgattgtt gtcgtcataa tgggtgatgc tgccatcttt
    20221 ctcgtgtttt ttcgcttcta cgctatcgcg ccagtcatag ctgattcccg ccacgacatc
    20281 gacattatca gcagcagccc attgatattc actggcgagc gaccaggtac gatcttcata
    20341 gcgatcgtaa gcggcgtgcg gcgcaccttt ttcccggtgt acgtcatctt tccagttaac
    20401 ggcaaacgac agcagatcgt tttcacgcac atcggctgcc agttgtaatc cggcaccgtc
    20461 gctgtaatcg gaataatggc tgtagctgcc ttttttattt ttcaaatcag ccagcgagtt
    20521 gtacatcatc agcgtatttt caaaggtgtc gcgatacagc cgacttttca gggtaaaacg
    20581 gtcgtttagt tgggtcgctc cctgataata aaaactttct ttgtcatact ctggccactg
    20641 ccagtagcgt gatttttgac cacaatttcc gctgtatggc gggttatctt tttcaccatc
    20701 ctgcttaatg taagtcagcg tgtattcatc gttttcgcgc ggtgtaaaac cgagcttcac
    20761 aatgccgcgt ttatcatcag ccgatgaatt aatcatcttg ccgtgtttgc ctgcaatatc
    20821 attatttaca ccatgcggca ggccgagaaa atcctgcttt agctggctac cgctgacttg
    20881 caaataccca agatcgctgc tggcggcaaa tgaagcatgc atatcgtagg cattgtcctg
    20941 actacggctc catccctggc gatatcccag acttgcttct tcagggtggt gatattaatg
    21001 gctccgccca tctgattagg cccatgaagc agcgacgaat accctttgga aacttcgact
    21061 gcccccagat tgttggtcag aatccgcgcc agatcgaggt tgccgtcata ggggacataa
    21121 atgggcacac cgtcgaaata gaccggcacc tgccgactat caaagccacg aacttttacc
    21181 tgttcttcgt tgcggctgcc tgacttttgc agcaccacgc cggggacgac acttaatgcc
    21241 tgagcgacat tctgtttatc caaggccttc atggtgggtt gatcgagaac ggtggtcgtc
    21301 gtcgatgata ccggactgga ccatacggtc agcgtgtcgc tctcctgagc cgctacggcc
    21361 tgctgggtaa aggcaagtga taatgctgtg cagagataac gttttttgag tctcatagaa
    21421 taaattccgt ttaacaaaga attaaataga ggctgtgggt tgcagactga ctttaatgtc
    21481 agctggtgcc gcgtaataag gcgcggaggt aataaaaagg cgaatgccgc agtcgaggta
    21541 atttttgagt gtcgtcagat taattccgcc ggtcagagcc agcgtgcagt gaggagccag
    21601 cgacggcgct atttgcgcaa tttcagttgc ctgttgcgga ctaaatttgt cgagctgaag
    21661 tacgtctggt tgtgcgcgta acgcggcgat agcctctttc ggcgtgtcgg cttcaacaac
    21721 aattttcttc tcgggtgcgt ggcgacgtaa ctgagcgatt gcgcctgacc agccctgatt
    21781 gtcatgaaga aaatggcggt ggttggcaaa caataatatg gtttccgcac atccggcacg
    21841 atgaattagt cctccggcag ccagaattgc ctgcgaagcc agtaagcggg tgcccggaat
    21901 tgcttttcgg gtgcaggcga tattgccgtc aggataacgt tcacgaagca atgccagcat
    21961 ttgagcgaga taatcagaaa caccgcaact ccactccagc acattctgga ctgccttcca
    22021 tccctgatga agtgctgcgg cattaccctg cgcacggatt agccgttgac ccgcgttcgc
    22081 ttgtgaaccg tcgctgaccg cgtcatcaat agttaatccc agcgtagtta acatcttaca
    22141 cgcgacagaa acgccgctga cgcagccgcc ctgacggtga aaaaacgcta tatagccagg
    22201 ctggtgtcca atatttaatg ctcgcgtggt caggtcacct ccctggatat cttccagcag
    22261 taacgcatcg atttgtgcct gagaaaggaa aatcatcaca gtgcctcctg cggtacgcta
    22321 tcccaccaga tgagcgccca gtcgcggctg gcaggggcga gggcacgagc atcttgttgt
    22381 tgataccagt gatataaccg ctcacgctca tcgtcgttaa gcgcgccaag actccagctg
    22441 acgttctgct caaaacgttc ccaggtgctg ttatcctgtt ggcaattttg tccacggata
    22501 aaatcaacgt gggcataaat tcccatctga tacaggacgt tgagcgcaaa gatatagttg
    22561 ggtaattcga tcacttcccg accagccgcc cgctgaatgg ctggagagac gaacgaggtg
    22621 ctgaccagat gagttgtata gacgcgaagc cgtgcctgat tattcagttt gctcattgca
    22681 tggcgcatat cagccaccag agtggagcgg gaagccacgg caatatcgca gcgtggcagg
    22741 tcactccagt cctcttccca ggcgcgctgg atccagtgga cattgtcagc cttcagtgtg
    22801 gcggcgcggc gggcggcaac gttcagcatc ccctgactgt aatcaacgcc ataaaccgta
    22861 gtgagtttgt cagccagcgc cagcgatacg gtgcctggtc cgcagcccat atcgaataag
    22921 gtctgagcgc cttgcaggtc aattttagcg atcagttgtt gcaggtagct gtccgttggc
    22981 gaggcgcaat tttccgccat tttctccgcc cgttgatccc agtgatcggg cgttttttct
    23041 gtgcggtgag ccagtttgag ttgctgcaga taaaggtctg caaaatcaat atcatcaatg
    23101 agcatgattg ttccttagcg gctaattgca gagaggtgat gatgaatttg gtcggcagaa
    23161 acccgataca gggccgccag tttgttcgtt gttaactgtt cggtcggtaa gccttgtgtt
    23221 actcgtccat ctggctctac ttggataatg ctgtcggcaa tggcgttggc atggagcgta
    23281 tggtgagtcg acattaacat tgtcatgccg ttatttttga gctgcgccag cgtgtccaga
    23341 agttggatct gatgaccgaa atcgaggctg gaggcgggct catccagtaa caacaagcgc
    23401 ggttgttgga cgagtgcacg ggcgattaaa gccagctgac gttcaccgcc gctaagcgta
    23461 ttccagcgtc gtgtcgccag atgcaaaatg cccagctttt ccagttgctc cgtggctttc
    23521 aggcgttctt gcttaccggg aacggaaaaa gcgcctatgg tgggggccag gcccatcaac
    23581 accatatcca gcacactgaa agcaaaaata ccgtcgtgcg cctgcggcac ccaggcaata
    23641 gcctgtgcgc gctggcgatg gctgagtgtc tgcacagaga ggctatccag taggatctcg
    23701 ccttttaata acggtataac gccaagaatg gagcgcatga gtgtggtttt gccgcaacca
    23761 ttcgcgccaa gcagacaaca aattgttcct tgcgacaggg tgaaggacac gtcgcgtagc
    23821 actggatggc gggagtaacc cagagacgca tgacgaacgg caagcaaagt cactggcgac
    23881 ctccgcgtaa caacaaaagc aggaaaaacg gtgcgccaac aaaagcggtc agaatgccaa
    23941 gtgggatttc tgttgttcca atgctgcgcg ccagcgtatc ggtaagcagg agcagaatag
    24001 cgccagtaca cattgccata ggcaacagct gctgatggtt atgaccggtc aacaagcggg
    24061 caatgtgcgg aacgaccaga ccgatccaac caattatgcc cgctattgct accgtgctgg
    24121 cggtgattag tgtggcgcag acaatcaatc caaagcgcag acgcgtcacg ttcagtccca
    24181 gcgaacgggc ttcgtcgtcg gaaagcgtga gtaaattcat gcgccagcgg agaaaaaaca
    24241 ggggcaagga gcctatgaga atgatcggtg cagcatagca gagatcgcgt aaggtcacgg
    24301 tggacagtcc ccccagcaac cagaaggtaa ttgacggtaa ctgggtgtaa ggatcggcca
    24361 gcgttttaat tagcgatatc cccgctccgc acaacgttcc taacgcaatg cctaccagca
    24421 ccagggttaa gatcggatcg tggcgggtca cccgacgcgt aatcaaccag acgcccgcga
    24481 ccaccatcag cccgccgcaa aatgcgtaca gctggatgta aacaatgggt aatccccaca
    24541 ggattgccgc gcaggcacca agtccggcac cggcggagac gcctaaaata tccggtgaaa
    24601 ctaagggatt acgcaacatt ccctgatagg tggttccggc tcctgccagc gctgcgccca
    24661 gtaacagcgc agcaagaata cgcggcacgc ggatttgcca gaagacaatt tgttcctgca
    24721 cagactcatt ttcttgacga agaattagcg ccagcagttg ctgcatatcg agatgataag
    24781 ccccacttac ggcggcaaaa gtgatactga tagctgccag cagcaaaata catccgttta
    24841 cgattctcat tagctcgcca ccaatttttg atagtcggca tcactgagtg aacaatgcca
    24901 gaacagttgg gcgtaatgct gcatgtcact tttaaactgg cgattgatgg cgggatccag
    24961 ccaggcgtga agtctgcgta atcccagaag acggttgatt cccggcgggg catcgagcca
    25021 gccaaagggc aagccactta aaaataagat gcgttgttcc gcaacggctt tcacgccctg
    25081 ccagagagga tcacgacgaa taaaatctgc agtaacggcc tcctgaacca gaataatatc
    25141 cggctgccag cgcaggagat tttccatgga aacctgtgtc aggccgtggc gatcggctat
    25201 ttgcgcgacg ttgtgcaaac ctaataattc cgccgcctcg gtatgcaacg aaccctgtaa
    25261 gcccgtttcc agccctcggg gaccgcgcgc agcataaaag cggaggttag cggcgggtga
    25321 ggtggcgaat gcctgagctt caccaacgaa gtggctggcg agattggctt gttctgcggc
    25381 gcggtgctct tcgcctaacg ttttgccaag cgttgttaac tgttctgctg attgcgccag
    25441 ttttccgtta agcaataacc aggggatttg tgtctgttcg ctaacctgcc gtgcctggga
    25501 gatcaaggtt tcatccgtat tgccgcaatc aacgaccaaa tcgggatgta acgccataag
    25561 cccttccagc gatagtgtgc tggcgcgccc ggcgagtcgt cccagcctgg gtaactggcg
    25621 aatgtgctct ggcaatggaa ttaatgcctg acgggcaaaa tcaaaagagg aaaaaccgac
    25681 cattttttct ggcgcgacag ccagcaagag caaatcggcg ggcgcgcctg cgctgacgat
    25741 gcgtgtaatg ttttgggctt cgggaagaga ttgcgaagca aaacgattaa ctgtttgtga
    25801 aaaggacggt agagactgca ggagcagggt agatgcaagt gcctgcgcaa acgtccgtct
    25861 ggttatgggc atggtgaaca tcctgaaaag taaaatcgat atatactttc atatatatcg
    25921 ataaaaatca tgttcaatat caaagtttag ctattgaaaa catgaagatg tgacgacttc
    25981 cgcaagcctg ttgaccctct ctatcattaa aaatgtattt atcaggtggc taatattatt
    26041 taaatcattt aaggctaaat tatttaaata tgaatgacat tattgttttt ttatcgtcca
    26101 cggttacgcg acgttgaagt tacttgtttt ataatgagcg atttttgata tctcgccgag
    26161 aaaattatca gcattctctc gtttcagtgt cgtgaatata ctgttaacgg aggtgaaaag
    26221 aatgctgggg gttatacaaa acctatgttg ttgtcgtccg gattcaccgc ttgctgatag
    26281 taagcagcgt aaccagaagt taccgcaaca agactttgag gattttaaga acgacgttat
    26341 taatgtcatt aacaacaatg aagatattta ttttcttaga gagagtaatt tactgaatgc
    26401 gtttattcgg acgtgcaaaa aaagagatga taacagttac ggaaatatga tatttacgct
    26461 ttattttgat aacgatatct atgggaacag aattatcaaa gcatcgctga acggcggaga
    26521 gttgcagtta ttaagcacag ggtgtagcaa tgataacatt ctc
//
LOCUS       DAJTSA010000053        26423 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000053 DAJTSA010000000
VERSION     DAJTSA010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26423
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.053"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..443)
                     /locus_tag="OO369_002353"
     CDS             complement(<1..443)
                     /locus_tag="OO369_002353"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5982606.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILV"
     gene            complement(589..1152)
                     /locus_tag="OO369_002354"
     CDS             complement(589..1152)
                     /locus_tag="OO369_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5982607.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(1359..2297)
                     /locus_tag="OO369_002355"
                     /pseudo
     CDS             complement(1359..2297)
                     /locus_tag="OO369_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(2360..2734)
                     /locus_tag="OO369_002356"
     CDS             complement(2360..2734)
                     /locus_tag="OO369_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HCR5982608.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(2738..3886)
                     /gene="wzy"
                     /locus_tag="OO369_002357"
     CDS             complement(2738..3886)
                     /gene="wzy"
                     /locus_tag="OO369_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCR5982609.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(3906..4811)
                     /gene="rfbG"
                     /locus_tag="OO369_002358"
     CDS             complement(3906..4811)
                     /gene="rfbG"
                     /locus_tag="OO369_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCR5982610.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(4804..5694)
                     /locus_tag="OO369_002359"
     CDS             complement(4804..5694)
                     /locus_tag="OO369_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCR5982611.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(5724..6980)
                     /gene="wzx"
                     /locus_tag="OO369_002360"
     CDS             complement(5724..6980)
                     /gene="wzx"
                     /locus_tag="OO369_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCR5982612.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(6996..7541)
                     /gene="rfbC"
                     /locus_tag="OO369_002361"
     CDS             complement(6996..7541)
                     /gene="rfbC"
                     /locus_tag="OO369_002361"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR5982613.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(7546..8424)
                     /gene="rfbA"
                     /locus_tag="OO369_002362"
     CDS             complement(7546..8424)
                     /gene="rfbA"
                     /locus_tag="OO369_002362"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5982614.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(8483..9382)
                     /gene="rfbD"
                     /locus_tag="OO369_002363"
     CDS             complement(8483..9382)
                     /gene="rfbD"
                     /locus_tag="OO369_002363"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR5982615.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(9382..10467)
                     /gene="rfbB"
                     /locus_tag="OO369_002364"
     CDS             complement(9382..10467)
                     /gene="rfbB"
                     /locus_tag="OO369_002364"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5982616.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            complement(10840..11733)
                     /gene="galF"
                     /locus_tag="OO369_002365"
     CDS             complement(10840..11733)
                     /gene="galF"
                     /locus_tag="OO369_002365"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR5982617.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(11908..13302)
                     /gene="wcaM"
                     /locus_tag="OO369_002366"
     CDS             complement(11908..13302)
                     /gene="wcaM"
                     /locus_tag="OO369_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR5982618.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(13313..14533)
                     /gene="wcaL"
                     /locus_tag="OO369_002367"
     CDS             complement(13313..14533)
                     /gene="wcaL"
                     /locus_tag="OO369_002367"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR5982619.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(14530..15810)
                     /gene="wcaK"
                     /locus_tag="OO369_002368"
     CDS             complement(14530..15810)
                     /gene="wcaK"
                     /locus_tag="OO369_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR5982620.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(15990..17468)
                     /gene="wzxC"
                     /locus_tag="OO369_002369"
     CDS             complement(15990..17468)
                     /gene="wzxC"
                     /locus_tag="OO369_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR5982621.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(17470..18864)
                     /gene="wcaJ"
                     /locus_tag="OO369_002370"
     CDS             complement(17470..18864)
                     /gene="wcaJ"
                     /locus_tag="OO369_002370"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR5982622.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(18919..20289)
                     /gene="cpsG"
                     /locus_tag="OO369_002371"
                     /gene_synonym="manB"
     CDS             complement(18919..20289)
                     /gene="cpsG"
                     /locus_tag="OO369_002371"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR5982623.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(20482..21918)
                     /gene="cpsB"
                     /locus_tag="OO369_002372"
     CDS             complement(20482..21918)
                     /gene="cpsB"
                     /locus_tag="OO369_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR5982624.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(21921..23144)
                     /gene="wcaI"
                     /locus_tag="OO369_002373"
     CDS             complement(21921..23144)
                     /gene="wcaI"
                     /locus_tag="OO369_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR5982625.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(23141..23620)
                     /gene="gmm"
                     /locus_tag="OO369_002374"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(23141..23620)
                     /gene="gmm"
                     /locus_tag="OO369_002374"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR5982626.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(23620..24588)
                     /gene="fcl"
                     /locus_tag="OO369_002375"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(23620..24588)
                     /gene="fcl"
                     /locus_tag="OO369_002375"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR5982627.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(24591..25712)
                     /gene="gmd"
                     /locus_tag="OO369_002376"
     CDS             complement(24591..25712)
                     /gene="gmd"
                     /locus_tag="OO369_002376"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR5982628.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRTSSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(25738..26286)
                     /gene="wcaF"
                     /locus_tag="OO369_002377"
     CDS             complement(25738..26286)
                     /gene="wcaF"
                     /locus_tag="OO369_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR5982629.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITISAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(26302..>26423)
                     /locus_tag="OO369_002378"
     CDS             complement(26302..>26423)
                     /locus_tag="OO369_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCR5982630.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT         7222 a         6580 c         5645 g         6976 t
ORIGIN      
        1 cacacaagga taaaatatcg tcccaacttg taatttgtaa tgttgatatc ttttattaaa
       61 cttgcaattg agatcgcaac ctgcaatact gacaatgggt aaatgggtga taaatacctg
      121 tcaccttgcg taacagaaaa ccaaaataag acatatgtta aaacaatcga gaaaaatacc
      181 caaagtattt tattattcct aagtgtaaag acagacagta tagcataaaa aacaacatca
      241 ctgccaactt tattaattgc ttgttgcaaa tctaatgtgg caggacttat accatgtcga
      301 gcttgttctg ctgtttgaac ttgtagatca atttcattcc ataggtagtg accaaatata
      361 tttcctccag caggactaaa aggatcacca gagatccaaa aactacggac ataccaataa
      421 atgccaccaa gaattgttgc caagctaaaa ataagaaatg tctgtatgtt tctctttttc
      481 gctatcaata taacaaatat gatggctcca tatatggcac caaatagttt tacgccacaa
      541 gcaattccaa taaaaaaacc acataaaaaa attaattcat tttgtgtttt attgttttct
      601 gagcgaatat atataagaat actggctgca gcaaaacaaa gatagcgtta aatcaatata
      661 tgcataacca agatgatctt taagtggatt gatagtaaat agtaggattg tagcaataat
      721 gcctggataa taagttttcg taatttccca tgaacagcca acaagtccac aggctattac
      781 aaaaaaacag atatttgcta aaaactgagc taaccttaca tctcctaact gtaaacctaa
      841 agccattaaa agatctgcat tttgcgggaa cagaggaaaa cgcaaatatt gttcaacaac
      901 aattgcatgg tgctcaacta atgaacgagc caaaggtaag tgatacatag tatcatccca
      961 atcaccggga gctcgcaggg acgataacaa tgtaaccaca aaaatcaaaa caagaaataa
     1021 aataacaaac agcgtactga actcaatatt atttattgcc agccatctaa attgctgacg
     1081 tttaataatg cttaaaaccc aacacaccgt aaaaattaaa aagatagcaa tcttattaaa
     1141 ttgcccgacc atgcccagaa agtaaattat caaaatattt aaagacatcc ctatgcatag
     1201 gcaataaaaa ataaagaaaa tttgttaagt cctgccaccc cctccttaaa aaaagatgac
     1261 aatacaatat taccaatgac aaaaaatgtc attccaaaag caaaacaata cacaacatac
     1321 aaatacaacg gcaattcaat catagtaaaa tactctattt acattatcag tttatagttt
     1381 ttttcagaaa gataaaataa tgccttcatt tcacgtctag ttgttgtatt tgaatgcaaa
     1441 ataatgccac atacaaatgc cattattgac gataacatga tggaggcagt taaaaaggca
     1501 gtcggaaatc ttctcaccaa acctgtatca atataatcaa caatgatggg caatcctaaa
     1561 attatagaca aagcagcaag taaaacgccg ataatactaa aaaagtatag tggccgttca
     1621 agcgagtaaa gtttaattat tgttttcaga atcttgaacc catcactcca agttgatagt
     1681 ttgctcacac tcccttcaga tcgctcgcca tattttgtat taacctcacc atacttcata
     1741 cgtaattcta aagcatgaat tgtaagctca gtttcgattt caaatcccct tgatagacat
     1801 ggaaaagatt taacatagcg tctggaaaat acacgatacc ccgacaacat atcagtaaaa
     1861 ttccctttaa agatagtctg aacagtacca gtcaacaatt tatttcctca gcgatgccct
     1921 tttctgtatg tattttgatc tccattctct tgacgacatc ctacaaccat atccaattga
     1981 ttttttatga gatggttaat catttctgga gcagaacttg catcataagt gtcatctcca
     2041 tcaaccatta gataaatatc cgcatctaca tctgagaaca tacgtcttac gacattcccc
     2101 ttgcctttca gaggaacaga gtgaactgtt gctccagcat cctctgccac ttgtgctgta
     2161 ctgtcagtcg aattattatc aaatacatat atagaagcag ttggaatatt cgttttgaaa
     2221 tcatttatga cttttgctat tgccttctgc tcattataac aaggaataat tacagcgata
     2281 ttaaaagagt gatacataag cccctcatat aaactattta gctaatgtgc aagtaattgt
     2341 gtttttaaca aatcgtttac taacgaaaaa tgaaatattt tgatgcgcaa tatcctaaaa
     2401 ccaaactaaa aagagaaaag gtgaacaatg taaatattgg agataaagct aagagatcaa
     2461 ataaaacgcc gaataaataa ctcatcgccc ccataaaaaa tataaatata aaatatctag
     2521 ccttggttgc agatacttta aatgagcatc ttgcatttgc aaaaaaagaa aaggttgctg
     2581 cacatataaa accaacaatg ttacttcggc cttgaccaaa agctaaatta ttataacacg
     2641 cataaaatac cccccaatgt aaagcagtat tgacaattcc aattaaaaaa taactaaaaa
     2701 aagaactggt caataacttc cctattttta acatccttta ttttgctcca gaagtgaggt
     2761 tattactaat ttggatattt tctatagaaa atacccataa aactacaacg cctataataa
     2821 aaattcccat gggtgtaaaa agaaatggat tagttataga aacggtaata ctgaaaaaaa
     2881 gtattaacga catcattaat gatgtttttg tactatttaa aaaatagcct ttcaaaaaga
     2941 taagcaacat aggaactaat gatataaata caccaataac gcctgttttc tgaagtatct
     3001 caagaggtac tatttcaatc cttaatcgat ctaatataaa tgaaccaaat cctcttccaa
     3061 acaagaacgt cgctaaatca acatttttat aataaaaata taaatcatta aatctaacag
     3121 agtcagaatc ttcactgcgt gatccgacca tgtaaagcat aattataaat agaacgctgc
     3181 ccaatattat tataatagta tttaaatata gcttatgtaa tttaaaactg gctaataaca
     3241 gtgataatgt tgtaaaaagt aatacacctc ttgattctgt caataatatc gcaagcacta
     3301 ttatgagaca ttttaaatat tttttgtcga caaaactgat aatcgcaccg acaccaaaaa
     3361 agtagaagga tttggaaaaa aaagccccat cgggacgaaa aaagaactcg ctagtcaatg
     3421 ataagtgttc ataaattaaa ttaaaattta acaaaccgaa attcaacaaa attatgtatg
     3481 aaatatataa cagtgatcca tataatgcaa atgcattaac tattttaata aaaatctcac
     3541 atgaaaatac ttttaaagta tatcttagtg taaatagata tataagtgac agcataaaaa
     3601 ggtatggttg cacatcaaac agtatatcat ttattttatt accagctaat aagccgacaa
     3661 agaagccata aaaaggaaat aaaagcaacc atataaaaag actgacacat ttttgggtga
     3721 ttgaaaaatt gaatgtaact aaattaaaaa ttaaaagtat aaaactaaat acaaataata
     3781 aatatcgcaa agggaatatt cccaatggct ccagtaatct tccaccacca ccaataatca
     3841 gttcaataca taaaaatgtt ataaaaattt tatttatatt attcatattc gtaaggtgat
     3901 gttttttaaa atatgtttaa aatcaacatt aataaaatat aaattttatc agcctttttc
     3961 cgccttaact tccaaaaact taaagccaag atcaaattgc ctatataacc tttacccaat
     4021 tgattattca caaagctatc atcagctaag gcattagcta ttttagttac ttcttttcga
     4081 taccacttgt ttctcaccaa accaagccgt ttgtattttg ctttaaaact aatgtttgct
     4141 cccacttggt tgttttcatg ctgtctgtac agcattgttg gttttctatc aatataccac
     4201 gaataatttc tcgtccgcgc aaatgaatat aaaaaccaat catgcagaca gatatcttgt
     4261 gcagcatttt tattatttat gatgaacttc ttaaattcaa taagcgtttc tttttttata
     4321 acatatgtac accccggacc tgctgcttca aaaaaataat cgaattgagt ttgcgactga
     4381 gccttatcaa ctaaattttt tcgccctgat ggataatatg caattacatt gctggaataa
     4441 caattcgcat tattaaatac caagcagtca attgcatttt ttattttatc tttataccaa
     4501 atatcgtcct gatcactaaa acagatataa tcatagtttt caagatcgac atctttaatt
     4561 aatctataaa aatttttacc tgccccccca aacttattga cgcttggaag tatatttata
     4621 attgagggat atgataattg aaattcttca caaatattta atgtattatc agttgataaa
     4681 tcatctgata taaaaattga gatgtctaca tctttttgtt caataatact ttttaattgt
     4741 tcctcaatcc agcattcgcc attatacgta gccataagta cagctactct tttttgatca
     4801 gaactatgca ttataacgac cgcccccagt aattcctctt attccatcca gcgtagccaa
     4861 aatatgtttt ttcatatatt caattttatt tttttgagtt gcgataatta aagtcgtatg
     4921 aattaaagaa aaaacaactt ccctaataga gagtaaaaga ggcacatgat tttttctaag
     4981 caaaagaaat gcatttctta ctcgataata acgacgtact ggcgagtgaa ttggtatgtt
     5041 aatcccacag atttttactg acttatcccc aatagcatga accatttcta tatcaggaat
     5101 gatatgaact aatatgccat agtttaagca tctaagacac cattctgtat cgacataatc
     5161 aataaaaagt tcctcatcca tcattccaac aatctcaaaa acttcttttg aaaccatggt
     5221 tccggatgag attgttactg aacttttaaa cggtgtctgt ccttcagtga catgaatttt
     5281 ctctcgaaga ccattttttt tgatattaca aataggataa taatgtgact tatctctatc
     5341 aataaaaact gggccagcta tttttgcatt ttcattatcc atatagcagg aaagtttttt
     5401 gataaactca ttgcgaatac ggctatcttg atcaaaaaaa attatcttct ctgccccttt
     5461 ttcaagagaa tatcgaatcc caatattttg tgcttttgct ataccttcat tcctaccaag
     5521 acaaattaaa tgaacttttt caatgtctgc taatttatct ttcaaggtga aattagtcct
     5581 attatcgact actacaacgt aacaattttg ctcctttagt tcagtgatca gtgcattcag
     5641 atttttaagt tcgggattat atgtcacaat gacagcgtaa atattactat tcattatcca
     5701 agtgactcag taattggtta attttagttt tcatatacag aacacattct agtataaagc
     5761 cattgtaata acagagcatc taaaattaca cgtatgctcc aggatattgc ggcacccagt
     5821 aacccatatt tttcgaccat aaaatacaaa agggctaaat aaggaaccaa ttcagcacaa
     5881 tgaatcaaag cagtaatttt agattttcct aatgcctgga ttgcagagaa tggtatcatt
     5941 gccagcgcat tgaagaaaaa accgatcaat aacacattta atatattaaa ggaattaatt
     6001 tgataattct caccaaacca tattttcaat acaagccctg aatataacaa gcctatgatt
     6061 atcaccggca aacagattag aaacattaaa agcaatgatt tatttacatt acgtttaaat
     6121 tctttaaagt ctttgatgtt acttaacctt ggaaacactg ccctcccgat tgctgcaggt
     6181 attattccta attttagaat aacctccgct ggtgcagaat aaaatgcaac tttatctgcc
     6241 cccataatat ttgacactat aaatcgatca aaatacacca tgaccggact tatgatatta
     6301 cttactgtca tccaaccgcc aaagaaaaag agtcgtttaa aggttttata acaaaaatga
     6361 acccccgaaa ttttaatatc attcctgaca taataggcag agactaaaat cgaaataact
     6421 cttgcaaata ttaaaccagc aaccgctgcc gacaatgtag cactataaaa aacaaatatg
     6481 gccggaattc ccgcgatgca agagcttgat atagattttt gaatatttac aatgccaaat
     6541 ttttcatccc cctcaagaat ggctgaccat aattgattta gaataaataa tggaatgcaa
     6601 atagccaaca gtttaaatgc tagttgtata tcactatgtt caacaccaga aatttttaga
     6661 taattaacaa ttccatcaga gaaaatcaat aataaaaaag caccaaagca tgaaaatagc
     6721 accaaaaaag atgtacttgt tgaaattacc ttggttcttt catgatgatt atcacgatga
     6781 attgcaattt ctctaataac agagcgagtc aggcctacat caaaaattcc agcataccca
     6841 acaagtgcaa ttgcaattgt ataaacaccg aaaccttcag gccccaaacc tcgagcgaga
     6901 aatcctagcg aggggatagc aattaaagtt ggtattgcat agccaaaaag gttccagaca
     6961 ctatttttta ttatgctcat tattacattt ctttatcata taaaataatc agcatctaca
     7021 aatttttgac cattaatatc tttatcagaa agcaatggat tttgaaccgg ccattctata
     7081 tttatacttt tgtcgctcca taaaatccca cgctcttgca tttttgaata gaaattattt
     7141 gttttgtaga caaattctgc ttcatcacta agaacgcaaa acccatgagc gaatccttcc
     7201 ggtatccata attgacgctt attctccgcc gacaaattta caccaaccca cttgccaaat
     7261 gttggtgatg atttccgaat atcaacagct acgtcaaaaa cttcaccgac aacacagcgt
     7321 accagttttc cttgagcata aggttccagc tgatagtgca gtccgcgtaa cacaccctta
     7381 gttgatttag aatggttatc ctgaacaaac tcaacctttc gtcctacagc ctcttcgaaa
     7441 actttctgat tgaagctttc cataaaaaag ccccgctcat caccaaatac ttttggttca
     7501 aaaattagca catcaggaat ttcagtttta attacgttca ttttattaat aacctttaat
     7561 cattttcagc agatactgac cataagcatt tttcttcagc ggctcggcta ataccttcac
     7621 ttgctcagca tcaataaagc ctttacggtg agcaatttct tccgggcagg aaactttcag
     7681 cccctgacgc tcttcaattg ttgcaatgaa gttgcttgct tcaataagac tttgatgtgt
     7741 ccccgtgtcc agccatgcat aaccacgccc catcatagcg acagacaaac gtccttggtc
     7801 catataaata cggttaatat cggtaatttc cagttcgcca cgtgcagatg gtttaaggtt
     7861 tttcgccatt tctacaacgt cattgtcata gaaataaagc ccagtaaccg catagttact
     7921 ttttggttcc tgcggttttt cttccagact aattgccgta ccgttatcat caaattccac
     7981 cacaccgtag cgctcaggat catttacgtg ataggcaaaa accgttgcgc cactctctct
     8041 gttaacagcg gtatccatta atttcggcag gtcgtgacca tagaagatat tatcaccaag
     8101 taccagcgca caatcatcac cacctataaa ttcttcacca atgataaatg cttgcgccag
     8161 accatccgga ctcggttgta ctttatattg cagattaagc ccccactggc ttccatcacc
     8221 aagcaattgt tgaaaacgtg gtgtatcctg cggcgtactg ataatcagaa tatctcgaat
     8281 acccgccaac ataagcgttg aaagcggata ataaatcatc ggtttatcat aaatcggtag
     8341 cagttgtttg ctcacagcca ttgtcacagg ataaagacga gtgccggaac caccagccag
     8401 aataatacct ttacgcgttt tcatttcacc attcctttta attcatctcg ctctagcatc
     8461 atgagcgaga tgcaataatt tgttaaattg ccgtggtcgt aaataattcg ttgagcatac
     8521 gtttcacgcc cacctgccag tcaggcaaga caagcgcaaa gttctgctga aatttttcgg
     8581 tattgaggcg agaattatgg ggacgacgtg ctggtgtggg ataggccgtt gtcggcacgg
     8641 cattaagttt gttaagagca aggttgatcc ccgctctgcg cgcttcttca aataccagag
     8701 cggcataatc gtgccaggtt gttgtaccac cagcaaccag atggtacaaa ccagcaactt
     8761 ctggtttatt tgcggccacg cgaatggcat gagcggtgca atcagccagt aattcagccc
     8821 ccgttggtgc gccgaattga tcgtttatta cagccaactc ttcgcgttct tttgccaggc
     8881 gcaacatcgt tttggcaaag ttatttcctt taccagcgta tacccagctg gtacggaaaa
     8941 taagatgctt tgcgcaatgt tcttgtaacg ctttttcccc agctagcttg gtttcaccgt
     9001 agacatttag cggtgctgtt gcatccgttt ccagccatgg cgtgtcaccg ttgcctggga
     9061 aaacataatc agtggagtag tgtataaccc aggctccgac ttcattagct gcttttgcaa
     9121 tcgcttcgac gcatgtcgcg ttaagtaatt gcgcgaaatt cggttctgat tccgctttgt
     9181 ctactgcagt gtgagcagca gcattaacaa taacatcggg gcgaattttt ttgacggttt
     9241 cagccacacc ttcggggttg ctgaaatctc cacaataatc agtggagtga acatcgagcg
     9301 caatcaagtt acccagagga gccagagcac gctgcagttc ccaacccacc tgccctgttt
     9361 tgccgaaaag caggatattc attactgacg gccctcatag ttttgttcaa tccacgactg
     9421 ataagtaccg cttttcacat tctcaaccca atttgtatta gccagatacc attccaccgt
     9481 tttacgaatc ccgctctcaa acgtttcttg cggtttccag cccaattcgc ggctaatttt
     9541 atcggcatca atggcgtaac ggcggtcatg ccccgggcga tcggcaacat aagtaatttg
     9601 ctcacgataa gatttctctt tcggtacaat ctcatccaac aaatcacaaa tagtgagcac
     9661 tacgtcgatg tttttctttt cgttatgtcc accaatgtta taagtttcac ccgctttacc
     9721 ttcggttacg acggtatata acgcacgagc atgatcctct acatacaacc agtcgcgaat
     9781 ttgatcccct ttaccataaa taggtaatgc cttaccttcc agagcattca gaataaccaa
     9841 tggaataagt ttttccggga agtgataagg accataattg ttcgagcaat tagtcacaat
     9901 ggttggtaaa ccataagtcc gcttccaggc acgtaccaaa tgatcgctgg aagctttaga
     9961 cgcagaatac gggctacttg gcgcgtaagc tgtcgtttcc gtaaatagcg gtaacgcttc
    10021 gttattattt gcttcatccg gatggggtaa gtcaccataa acttcatcag tagaaatatg
    10081 atgaaagcgg aaactttttt tcttttcatc attcagagca gaccagtaat tgcgcgccgc
    10141 ttctaaaaga acataagtcc ccacaatatt ggtttcaata aatgccgcag ggcccgttat
    10201 tgaacggtca acgtggctct ctgccgccag gtgcatcacc gcgtctggct ggtgctgtgc
    10261 gaaaatacgc gacatggcta cagcatcgca gatatctgca tgctcaaaag cataacgttc
    10321 agaatcagaa acatcagcga gtgattccag gttcccggcg tatgttaatt tatcgacatt
    10381 aacaacacta tcttgcgtat tatttattat gtgacgaaca acagcagaac caataaaccc
    10441 tgcgccacca gtaacaagta tcttcacttt tttattccat atagctatag agcatgctgt
    10501 gaaatagact gctccagatt tgattaatag aggtattaat acacgctacc gcccctggct
    10561 taacagctac cagtgcactg catgcatctc aacgatgtgg cgagcgttag ccactcgcgc
    10621 caaatccgaa aaattcaaac gttaattgtc ttaccaaaca gccgtagaaa caaggaaaat
    10681 cctggaaaaa tttgaataat atgagctatg taacttcttg tttttatagg ctttataaaa
    10741 acggcaacgg caggaaaatt caatgagcaa aacttatgcc acgaatcttc actgccgtta
    10801 taattttctt atcaaccgtt acatccggtc agattttcat tattcgctta acagcttctc
    10861 aataccttta cggaacttcg ccccttcttt caggttgcgt aggccatact tcacaaacgc
    10921 ctgcatatag cccatttttt tgccgcagtc gtaactgtcg ccggtcatca gcattgcatc
    10981 aacggattgt tttttcgcca gctcggcaat agcatcagtc agctgaatac gtccccatgc
    11041 accaggctga gtacgttcca gttccggcca aatatcggca gaaagcacat agcgacctac
    11101 ggccatgatg tctgagtcca gcgtctgcgg ctgatccggt ttttcgataa attcaacaat
    11161 gcggctgact ttaccctcac ggtccagcgg ctctttagtc tggatgacgg agtattcaga
    11221 gaggtcaccc ggcatacgtt ttgccagcac ctggctgcgg cccgtttcgt tgaaacgtgc
    11281 aatcatggca gcaaggttgt aacgtagcgg gtcggcgctg gcatcgtcga tcacaacgtc
    11341 tggcagtacc acgacaaatg ggttgtcacc aatggcaggt cgcgcacaca aaatggagtg
    11401 gcctaaacct aaaggttcgc cctgacgcac gttcataatg atcacgcccg gcggacagat
    11461 ggactgtact tccgccagca gttgacgctt cacgcgctgc tcaaggagtg attctaactc
    11521 ataagaggtg tcgaagtggt tttcgaccgc gttcttggac gcgtgagtta ccaggaggat
    11581 ttctttgatc cctgcagcca caatctcgtc aacaatgtac tgaatcattg gcttgtcgac
    11641 gattggtagc atctctttgg gtatcgcctt agtggcaggc aacatatgca tcccgagacc
    11701 cgctacagga ataactgctt ttaaattcgt cattatttca tccacctgta aaatggttgc
    11761 tgaattatag cttgttcgat ttttttcgcc agcatcaatt accctgaatt gattactgaa
    11821 ttacttgtga tgttacgccg cttcgttgtg gattgcagta gcattgttcc taagtatggc
    11881 tccatttttc caggaatggt cgcaaatcta ctccctccgt tccggcaatc taaagttaat
    11941 cttttccaca ttaacaatat ggtgattaat cctgtcgata tcgacggagc tttgccctct
    12001 ttcattcacc gcatgaacat ttgcaagaga cagcagtgtt tcttttttcg ccataaagac
    12061 gccgcgaacg tctttgcgca tgtcgaagtt catgctcaat gctggtccaa ctgaggattc
    12121 ctgcatcaca ttgatatttc gcataaaaag atgttgcggc ttgttgtgta actccagcga
    12181 ggcacgcttc atctcaatgt tagttagcga cacaaaggag acggcattcc cggcggagat
    12241 ttggatgccg cgcaatttat aagcaagatg ggtattatcc agttgaatat tattcactcg
    12301 gaaattttgc ggtatcgaga gatatttgcc tttaattacc ccatagccga ttaacatccc
    12361 ggcgctatta atcatttcaa tattatcaat cacgaaattg tcacaaccgt aaatagcgac
    12421 tgtcgcgtta tcaatgcccg ctttcttact gaaatccggc gtgatattgc gggcattgat
    12481 attacgaata acaaaatgtt taccattttc aacatggatc aactgccgac aatccgatcc
    12541 cgtgatattc gccacgacaa agtttttcac tgcctggtct tccgggtagt tgttatcata
    12601 agtgcttccc gcaaggccta tgccgatgcc ccagttgatt ttgccgttgg tacagttgat
    12661 acgctcgatg acatggtcag atatcaaaat atcactgtcg ttaattgcca cgttccattc
    12721 aatggcgtcg ccctgtaagt cgctgaactt acagttggta atgttggcac cgataatctg
    12781 gttatgaaat ccctggcgta agatggcgta attagcgtgg ctgacagtga ggttatcgat
    12841 ggtcaggttg cgcatgaccc gtttgttttt gccgccaata taaatctgca ttaccgggcc
    12901 aaagccgctc atcgccagcc ctttgatggt gcagtcagaa ccacgcacat ccagggtaat
    12961 gttatgcata ccgccgccct ctccccctgt cacctggctg ccgtcctgta agacaaatcg
    13021 ccctctgccg ttgccgcgca ggcttccaag gatgtgtaac gttttgccgg gagggatgaa
    13081 gatgccggtg ttgatattgt cacaaacgaa tccggcaggc acgacgaccg tttgcccttc
    13141 gctgaaggct tgtttaaatg aggcgatcca gtcatgtggg ttgtagtcgt taatgttaac
    13201 gctttgtcgg gcgggaagtg cgcgagcgaa aggggtatgg aggaaggcaa gcgccgagct
    13261 tgccgtcagg aaggtgcgtc gggagagtgt tttaaatggc atacgttctc ctctataaag
    13321 cctgcagcaa gctggcgagt tctcgattaa tcacctgctg gttaaaatcg tgttcgactt
    13381 tttcgcacgc acgtttgacg accggagcca gttcgtcggt atccagttgg ctaaacgccg
    13441 ccagacgttg tgccagtgcg cgagcatcgt tctcaggcag cagccagccg gatttatcgg
    13501 cctccaccag ttcaggaatt ccgctatgca gagtagaaac caccggaata ccgaccgcca
    13561 tcgcttccat tagcgccacc ggaatgcctt ccatatcgcc atccgcgccc gttaccgatg
    13621 gcaacaggaa gacatccgcg tcgtcgagca tctccttcac ttcatggctc ggtttaaagc
    13681 ccggcatctc taccacatct tccagttgat attgttcgat gatggtgcgc aggcgtcgtt
    13741 cccacaggcc aatgccgagg atgcgatagc gaaatgccac gccctgctct ttcaactgac
    13801 ggcaggcttc aatcgccaca tgcaggcctt ttttctcggt taagcgtgcg acagatatga
    13861 tttccagcgg cgttgcgggc gctttcaccg gacgcgggct aaagcgcgtc atgtccacgc
    13921 ccatacgcga tacggcgatt ttttctctgg ggcagcccat tttttgcagc cttccggccc
    13981 acagatcgct tatcggtaac atcaggtcgc cacggcgaaa cagttgttga tattcgggag
    14041 tgtagtggtt gagcacttcc cgactggaga tatcaatacc gtggaagatg gtggcaattt
    14101 tgccgcgaat gatacccagt tcgcgtagtt ttgctgcggt tacccccgcc gggccaaaat
    14161 gagcgatgaa cacatcggcg cgaaacggtg ttgcgacctg accacaaatg gcagacaaaa
    14221 tcaggttccg cgactcggca ccatagcgtt tgaggttgag cgcatgccag gtatttttac
    14281 gatgaatgcc acgtaaggtc tggctggcgc ggtggcgcag tttcgctact ttgcccgcag
    14341 gttcgtcctg taaccagcgg gttctggcga caaggttgta tttcgtccat gccgcgtggg
    14401 tgttctctgt atcgcctttt tgcaaagcga caatctccac ttcaaagccc atatcaataa
    14461 acgcggtaat ttgattgagg acgaaggttt ccgacgacag cgggaatttc aataagaaga
    14521 agccgacctt catttcacct ccccgatgcg ctcaagcaca gactgcacca tctgcattcc
    14581 cgtctgacgt tcgcgactga cggcttcgtt aaatcgagcg ttcagcaccg gaagctggcc
    14641 taaggtatcc gcaaccatcg cttgcaggct gccgtccagt aaatgacgaa tatcaatcgc
    14701 catctctggc agtcccagct gctgcataat cccggcggat ttatgttcat agttgatggc
    14761 aatcgccgga gtagcaaagt tcattgagat aatggcggag tgcaggcgcg taccgacggt
    14821 gagttcacag gctccgagaa ttttgcccat ttccagatcg ttgagttcat ccatcactac
    14881 gtggtaacgg gcaggatcgc tgatgtgctg gcgcagattg agcgccacca tgcggtcgtc
    14941 tttgttatag ctgtcgatac ccgtacaggt ggagagcgca atcacctgat agccttcatc
    15001 gagaatgcga ttgaccaccc cggcaaaggc tttttcatac gcttgttgag tggtgccgag
    15061 acgtttgtcg aacggtgcca gttcgcgcag ggtaattgcc actgttttct gttgtgcggc
    15121 aacgtccagc cagtgttgaa cggcatagct ggcggtgaag tcttcggtgt ggtgatcgac
    15181 cagccacgcg gtatcgacgc catgttccac ttttgcggtg gtgatatttc tgcgtttcat
    15241 caaatcaagg ctgaccgatt cgcgcaggat cagcgcgtcg cagtgaccaa agacatagtt
    15301 cgccagttgg ttaaattgct catcctggaa ggggccgacg ctgtgaccaa tcataaacag
    15361 cggctttttc gccataaatg tgcaaagcgc atgttcaaac tgcggcacgc cgtagagatc
    15421 gacaaaaaac gatccgccga cctgaataat ggcgtcgtaa cctgacagca ggcgcacgaa
    15481 gtcggtgaat ccctgggcga tggcgatatt gcgcagcttg ccagtgtcgg tgacgcgtga
    15541 gagcaatacc tgatgctggt agcggcgacg gagaactttt ttaatgcgcc cgacaacgcc
    15601 cgccgcgcta ttgtgttgtg tcatctgcag gaacagcgga tcgcccatta ccgggcggtt
    15661 gagcagccag gaagaactga ccggatagcg gctcatcacg tccacttcgg tatgtggatt
    15721 gaggatgttg atggcatcaa gtaagccgcg caagatggcg ctgtcgccac gattgccgca
    15781 agtgtggttg cccagaataa gtaatttcat tttttcctca taaatttgat gccaggtgag
    15841 gctgtgtttg ttgtgttcgt tgccgttttt tgcctgatgc gacgctggcg cgtcttatca
    15901 ggcctacagg tcccgagcac agaaccgtag gacggataag gcgttttacg ccgcatccgg
    15961 caaccgttgt cggaaccgaa aacagcaatt caccccgccc gtaaaagcat tttcattttt
    16021 tcgctgcgac aaaactgacg cttcacttcc accaccagcg gatggcgtga cagcacaatc
    16081 atcaccacaa acgccagcac gcccgcggct atttgcaccg ccagcagcat ccccagcgcc
    16141 agttgccctt tcagcacaat gcccagcgca taactgacca ccagcgtcgg caacgagaga
    16201 taaaacggca gccacaaact caggatgtac tggcgataac tggaaccaag aaccggttta
    16261 atcatcacga agtaactcag aatggtgttg ataatctgca ccagcaggaa accgagcgtg
    16321 acgccgattg cgcccgccat ctgcccacca ataacaatcg ccggaataaa cagaaatgtt
    16381 ttgaatacgt tgaatttaaa gctgatatcg acccgcgctt tcgccatcag cagcgaacca
    16441 atcgggttac ccacggagcg cagcagaccc acaatacaca gcaattgcag caccggaata
    16501 atgctgttcc acttttcgcc gaataccagc ggtacgaagt tattcgacac caccatcagc
    16561 cccagcagcg ccggaaagtt gataatcccc actatcgaca gcagcttgta gaagttaaca
    16621 cgcagctttt cggtgtcgtc ctgaattttg gcaaacgccg gaaacaacac gcgggtgatg
    16681 attgggttca gcttcatcgg cggtacgacg gccacgttgt acgccaggtt gtatccccct
    16741 gccacgcccg cgccaagaat acgcgccagc accagcgttg aaaggttggt gttgagatag
    16801 ttgatgatgc tgtccgccgt cagccaggca ccaaaacgta agttcggtgc cacagacgcc
    16861 agcgagaagt gcagaccggg gcaataaatt ttgcgtccaa agtagccaaa cagcagtgtt
    16921 ctcaccgcac tattgaccag ataaccgagg atcgcggtca tcgccagcgg ccagaaatgg
    16981 gcgctaacta ccgtaaaagt gaagcccgcc agcaccgcgc tggtttcgat catgccgatt
    17041 ttgttgaact ccagctcctt ctgcatcaac gcgcggaact gttgcccgtg ggggatcacc
    17101 acaaacgcca gcgacaatgt tttaatcaac ggtgccaggt ccgggttatt cagcacgtcg
    17161 ccgatgacat cactcaacaa aaacaccgcc acacatacca caagccccag cccgacgttc
    17221 agccagtaca gcgtggtgag ttcaaggtga ctgatttctt ttcgctgaat aatcgagtta
    17281 gcgataccga agtcagaaag cgtatctgcc agcgcgataa tcaccagcga cacagtcagc
    17341 aggccgaact ggtggttgtc gataatccgc gccagcacgg tcatctgcac cagcccgagg
    17401 ccgatgatga tcaccgtggc aatagccgac cacttcgcgc cgctgatggt tttttcacgt
    17461 aagctcatat caatatgccg ctttgttaac gaagcccttg aataccgtca ggaaaacgat
    17521 tttgatatcg aaccagacgc tccattcgcg gatgtactca aggtcgaact cgacgcgttt
    17581 ttccattttc tccagcgtgt cggtttcgcc gcgccagcca ttaatctgcg cccagccggt
    17641 aatgcccggt ttcactttat ggcgcagcat gtagccttca atgagctggc gatactgttc
    17701 gttatgcgct actgcgtgcg gacgtggacc gacaatcgac atccccccgg tcagcacatt
    17761 gataaactgc ggcaattcgt ccagcgaggt gcggcgcaga aagttcccca ctttagtgac
    17821 gcgcggatcg ttctgcgtcg cctgggtcac cactttgtcg ttctccatca ctttcatgga
    17881 acggaacttc cacactttga tcggcttgcc atccatacca tagcgagtct ggcggaaaat
    17941 aaccggccct ggtgaactga gtttcaccgc cagcgcaata cagcacagca ccggggagat
    18001 cagcagcaga ataagcgtcg ccagcacaat gtcttccgca cgttttagca ggcggttaac
    18061 ccccgaaagc ggcgtgtcat acagcggcac caccggaacg ccgttcatct cttcgagacg
    18121 tgaatggaga atgttgaagg taaagacgtc ggggatcagc agcaccgaac aggtggtatc
    18181 cgccagttga tggaccagtt ttttcactcg tgcgccgtcg cacatttgca tcgcgatata
    18241 gacgttatga atcttgcccg cttttgcatc ctcgaccagc tgttgcaggt tacccgccca
    18301 gtcgttagaa acgccgcccg gtttcgggtc gtggtaaacg cccaccactt caaaccctaa
    18361 ccacggctga ttacggaaac tctccatcag catttgtccg gcggctaaat cccccgctac
    18421 ggcgaccatc cgcttgttgt agccatgatt acgcagccag cccgccccaa tgcgaataca
    18481 tgaacggcaa accaccagtc cgatgctggt cagcccatac cacgccagcc agattttcag
    18541 ttgcgtgtcg aaatcattgt tgaacgccac cagtccggcg ctgaaaatca cgcttaaggt
    18601 ccagttttgt agcaacaggg caaattctgt cgctgcccga acaccgcgcc atgagcgata
    18661 aaaatcggtg atgccgccca gcatctggaa caccaccagc gtaatcagcg ccaccaacag
    18721 gtgcatgtag aggaatgaca gtccgctgac ttcgcaaacc agccatagcc cggcaaacat
    18781 gatggtgata tctgaaaagc gttgcaccat agagattaac gatgcattgg ttttcgctcg
    18841 ctcgcgcttt tttagatttg tcatcgttgt tcctgttatt agccccttac ccgcagtggg
    18901 gtaagggaag atccgacatt actcgttcag caacgtcagc agagttcgcg ttcgcgcttc
    18961 catcagcggc acatcaccgc gcgattccac attcaggcgc accaccggtt cggtattgga
    19021 ggtgcgcagg ttaaagcgcc agtcggcaaa ggtcatgctg atgccatcgc tgcgatccac
    19081 cgccagcgcc tcacggctaa aatgctgttc cacgcggtta atcgcctcaa cgggttgcgc
    19141 cagtttgctg ttgatctcac cgcttgccgg aaacgccgcc atccggtcgc gcaccagttc
    19201 gcccagcgtt tttcctttca ggcacaccag ttcggcgacc agcagccacg ggatcatgcc
    19261 gctgtcgcag taagcgaaat cacggaagta atggtgggcg ctcatttcgc caccgtagat
    19321 ggcgtcttcc ttgcgcatcc gttctttaat aaaggcgtgt ccggttttcg acattaccgg
    19381 cgtgccgcct gcagcagtca ccacatcaat agtgttccaa gagagacgcg gatcatggat
    19441 gatcttcgcg ccgggatttt tttcgaggaa cgcttctgcc agcaggccga caatgtagta
    19501 gccctcgata aactgccctt tttcgtcaaa cagaaaacag cggtcaaaat cgccatcaaa
    19561 ggcaatgccc atatccgcgc cgtgtttgat gaccgcattg cgggtgtcgt cgcgacattc
    19621 cggcagcagc gggttaggaa taccgttggg gaaattgccg tccggtgtgt tgtgtacttt
    19681 gattaattcc acgggcgcgc cgagggcttt aaagcgggct tcaatggcgt ccaccaccgg
    19741 acccgctgcg ccattcccgg agttgatcac cagcttgagc ggcgtgagat ttttgacatt
    19801 gatataaccg aacaggtgat caacgtaagc gtcacgcagg ttgatttgct gatagcgacc
    19861 gcgtttggtt tcatcgacgg gaggaaagtc gttggcttca gccagacgct ggacgtcgcg
    19921 cagtccggta tcaccgctga tcgtgcgagc cccctcgcgc accagcttca tgccgttgta
    19981 atccatcgga ttatggctgg cagtaacttc aatgccgcca tccacgccga gatggaacgt
    20041 ggcgaaatag atctcttcgg tgccggacat accgatatcc agcacatcga cgcccgcatc
    20101 ctgtaaacct tttgccagcg ccagttttaa ggtttcgctg gtgagacgga catcaccgcc
    20161 taacacaatg gttttcggtt tgagaaattc gccataggcg cgaccaatgc gccaggcgat
    20221 atcttcattc agttcttcgc ctaatttccc gcgaatatca taggctttaa agcaggttaa
    20281 ttttttcatg tcgttaccct ttttcaggca atagttggcc ctgaccgaaa cggccaattt
    20341 tatttttgtc gttatttaca ccgcggtttc gcattcattg cctgatgcga cgctgccgcg
    20401 tcttatcagg cctacaaaac cgaaccgtag gtcggataag gcgttcacgc cgcatccgac
    20461 aaacagcgcc tgatgcgacg tttacacccg tccgtagcga tccgcgaagc gcaccacatc
    20521 atcctcttcg agataagagc cggagcgcac ttcaattaaa tcgagcggaa ttttccccgg
    20581 gttttccagg cagtgcgtcg cccccagcgg aatataaatg gactcgtttt caccaagcag
    20641 tttgatatca ccatcaatgg tgacttttgc cgttcccgcg accactaccc agtgttccgc
    20701 gcggtgatgg tgcatctgta ccgacaaacc ctcgcccggt ttcacggtga tgcgtttcac
    20761 ctggtagcgg tcgcccgcgt cgatagagtc atatttgccc cacggacgat acacttcgcg
    20821 atgcacccga tgctcatggc gaccatcggc tttgatctgc tcgaccactt ttttcacatc
    20881 ctgtaccgcg ttacggtcgg caatcagcac cgcatctttg gtctgcacca ctaccagatc
    20941 tttcaccccg acggtggtga ccaggcctga ttcggcatac acatagctgt tttcagtttt
    21001 gtgattaatc acatcgccgt ggcaaacgtt gccctcggcg gtgtgggcgc tgatctccca
    21061 taatgaagac caggaaccaa catcgctcca gcccgcatcc atcggcacca caacagcatc
    21121 tgccgtaggt tccatgaccg cgtaatccac cgactcttcc gggcaggcga gaaacgcttc
    21181 ttcatccaca cgaataaaat cgagatccgg atcgacggcg ctcatcgctt tttcgcaggc
    21241 atcgaggata tccgggcgat attttttcag ttcttcgaga tagcgtccgg cgcggaacag
    21301 gaacatgccg ctgttccagt aatattcgcc gcttgctaca taggcctggg cggtttccag
    21361 attcggtttt tcgacaaact gcgccacttc aaaggccacc gtatcctgct cacccacaga
    21421 cacttcaccg cgacgaatat agccataacc ggtttctggt agatccggca caatgccaaa
    21481 ggtcaccagc ttgcccgctt cggcataagg catggcatta cgcacggcgg cacggaacgc
    21541 gtcttcatcg gcaatcacat gatccgccgc caataccagc attaacgggt cgctctccgg
    21601 gctatgacgt tttgccgcca gcgccgccag tgcaatggca ggtgcagtgt tacgccctgc
    21661 cggttcgaga ataatgttct cggtgagttt gttcagttga cgcagctgtt ccgcgacaat
    21721 aaagcggtgc tgctcattgc aaatcaccac cgggctttcg cactccacgc cgttcaggcg
    21781 gcagatggtg gtttgcagca tggtgagatc gcctttcagg cataaaaact gcttggggta
    21841 aagtacgcgg gaaagcggcc ataaacggct accggagcca cctgccatca caactggata
    21901 gagtttcgac tgcgccataa ttatccccga atatcattta taaattgacg taacacgttc
    21961 tctttatcga gcgtgcgttc ggcatattca cgtgccaccg tgttatgttt gggcagcagg
    22021 agcgcctgac gaatccccgc caccagcgcc tcgaccgatt ccggttctac gcaaaccgca
    22081 atacccggaa aggtttcgca aagctgcccc agttctgtgt gggcctctgc ggtaatcacc
    22141 gcgttaccgc ctaccgccag aatattggtc agtttcgacg gcaatacggc atctgccgcg
    22201 ccgcgttttt gcaccaccag atggcaatcg cccatcttca gcagtgcagg taaagcgtca
    22261 tacgattgca gcgggaaaaa ttgcatgttg cgcagtccac gctgctgcgc cattttttcc
    22321 agccgcgctt tgccgccgcc ctgcccgaca atggcaaaaa tcagcggttc atcgcgcagg
    22381 agatcggcag cttcaataac gttttccagc ccctgctttt caccaatatt gccggagtaa
    22441 agaatgattt ttttgttatc cggcaggcca agctggttac gaagggcatc aacatcggcg
    22501 tctgcaacat gctgaaaacg ggcaatttcc gaccagttgg ggaagaagat gacgttatcc
    22561 gccgccacgc ctttttcgat ggctttattc atcatcgaac gcgaaatcgt ggaaacgtta
    22621 tcgacgttat gcagtccgct acgttcgaac gccgttgcca gctgtgccac tttgccgcct
    22681 ttgccttttc cggcaaggcc cagccccagc atggcgtcca cttcgtaatc ctgaatatgc
    22741 agcacggtac gcgcaccaga gagcttcgcc agcaggcgca ttcccggcgt gcaaaacagc
    22801 gttggcacta cgccaataat gcgatccggc ttccagcgac gttgcgccat cagtggaaag
    22861 aaactgctga cggcaaaact gccgagatgc aacaagcgtt tcagggtgct cggctgtttt
    22921 ggcacgtaca gcgggcagcg ccacaccgtg gcggcccctt cttctcgttt gtagcgccag
    22981 gcggaatagt tctcacccac ctgccattgc gggtagtaag gcggtgcggt aatgacccgc
    23041 acctcatgac cttgtgccgc caaccattcc accatctcgc cggtgtattt gccgatgccg
    23101 gttaactccg gcgagtagtt aatgccgtag accagtattt tcataatccg ggtactccgg
    23161 cacgcttctc agcgagaaaa taggctcggc tgttagcgtg aacatcgtta ctggcgagca
    23221 gcgcgtccgg cgtcagccag cggtaatcgt catgctgctc atccggcagt aacagctctt
    23281 cttccgctac tctgaagcga aaaccgagca ccacatagtg agtggtgaaa tccgtgccgg
    23341 agaagttatc gtcataaaaa tgctgccaga caccgtaaaa ctggcctgct gttatcggca
    23401 gacgcagccc cagttccgcc atcgtcagcc gctcaaatgc ggcttccagc gtttcgtctt
    23461 tctgcacgcg ccctcccggc acaaaccagt aaccctgcgc cgggcggttg gttcttttgc
    23521 caagcagaaa ctcgccgcga ctgttctcga caataaagtc gagagagaca agcggtgtgg
    23581 agcgcactac agtggcaaag tcttcctgac gtaaaaacat cattcccccc gaaagcggtc
    23641 ttgattctca aggaaccact ggtaagtgct ggcaagcccc gcttccagtg agatttcgtg
    23701 ataccagcca agctgatgca ggcgcgtcac atccagcagt ttgcgcggcg taccatccgg
    23761 tttgctggca tcaaaaacca cccgaccttt gtaacccacc actttggcga tggtttgcgc
    23821 cagctcgcgg atagtgcagt caacgcccgt gccgacgtta atgtgcgaca gcatcggttg
    23881 ggtattctcc agccagactt catgcgccag ctccatgaca tgaatgctcg ccgccgccat
    23941 atcatcgacg tgcaggaatt cacgcatcgg tgtaccgctg ccccatacca ccacgtccgg
    24001 cgcattctgt gccgtcgcct cgtggaagcg acgcagcaat gctggtatca catgggagtt
    24061 actcgggtgg aagttgtcat gcggcccgta caggttggtc ggcatgacag tgcggtaatc
    24121 gcgtccatac tggcggttgt aagattcgca cagtttgatc ccggcgattt tggcaatggc
    24181 gtaaggctcg ttggtcggct ccagcgtgcc ctgcaataac tcgctttctg ccatcggctg
    24241 ttttgccagt ttcgggtaga tgcaggacga tccgagaaac atcagtttgt tcacgtcgtt
    24301 ctgatgcgcg gcgtgaatga tgttgctctc aatcatcatg ttctggtaga tgaaatccgc
    24361 cggataggtg ttgttggcaa caatgccgcc cactttcgcc gccgccagat agacctggtc
    24421 aatgcgttcg ctggcaaaga aatcatgcac ggcgcggctg tccagcaggt tcagttcatc
    24481 gcgggtacgt aataccagct ccacatcacc gcgctgttcg agctgccgct tgatggcaga
    24541 accgaccatc ccacaatggc cagcgataaa aatgcgttgt ttactcatgc ttatgactcc
    24601 agcgcgatcg ccacgtcgta gccgtgagat ttcagcagag agtgtttttt cgccgcttcg
    24661 aggtcattag ccaccatttc agacaccatc tctctgaggg tgatttccgg tttccagccc
    24721 agtttttcgt gcgctttggt cgggtcgccg agcagcgttt caacttctgc cggacggaag
    24781 taacgcggat caacggcgat aatcacatca cccggtttaa cgcccggcgc gtcatgcccg
    24841 gtgacggaaa ccacaatgcc cttctcttca acacccgtgc cttcaaagcg cagtttgatg
    24901 cccagctgtg ctgccgccat ttccacaaac tgacgtacgg agtactgaac gccggtcgcg
    24961 ataacgaaat cttccggctg ttcctgttgc agcatcatcc actgcatttt tacgtagtct
    25021 ttggcgtggc cccagtcacg cagggaatcc atattgccga ggtacaggca cgactccagc
    25081 ccctgggcga tgttggcgat tgcgcgggtg attttgcggg taacgaaggt ttcgccgcgg
    25141 cgcggggatt catggttgaa gagaattccg ttacaagcgt acatgccgta ggattcgcgg
    25201 tagttaacgg tgatccagta ggcgtacagt ttggcgaccg catacggaga tcgcgggtag
    25261 aacggcgtgg tttctttctg cggaatttcc tgcaccagac catacagttc agaggtggaa
    25321 gcctgataga aacgagtttt cttttccaga ccgaggaagc ggatcgcctc cagcaggcgc
    25381 agcgtaccca tcgcgtcgac gtcagcggta tattctggtg actcaaaaga gaccgcaacg
    25441 tggctcattg cgcctaggtt gtacacttca tccggctgta cttcacgcaa aatgcgcgtc
    25501 aggttagagg tatcactcag gtcgccataa tgcagatgga atttcgggtt gcaggtgtgc
    25561 ggatcctgat aaatgtgatc cacgcgctcg gtgttgaatg acgatgtgcg acgcttaata
    25621 ccatgcacct cgtaaccttt ttccagcaga aactctgcca ggtaagaacc gtcttgtccg
    25681 gttacaccgg tgatgagagc gacttttgac atgtcttatt cctctgtatt tttgaattta
    25741 ttcagtttca acgcgttcgc gtatcaccac tgcgggattc ccccggcaaa ccacatttgc
    25801 cggaagcgat ttaaaaacac tgcttcgtgc acccacgacg gtgccgtcgc cgattgtgac
    25861 gcctggggca acaaagacat cggttgccag ccagcatttc tcgccaatca caataggcgt
    25921 ggcgttaatg gtgaaatgtt gacttgcatg gtcgtggcta ccggtgcata aataactttt
    25981 ttgcgatatc accgaatgtg cgctaatggt tatttcaccg agggtatata aattgacgtc
    26041 atcgccaacc cacgcgtaat cacctaaggt taatttccac ggataggtaa tttttactga
    26101 cggacgaata actacgtttt ttcctatttt tgctccgaat aaacgtaata aaaaagcccg
    26161 ccagcgatac aatacttgtg gcgaccaggc aaatattgtt gcctgtactg cccaccataa
    26221 ttgcacttta atagcgttgc cgccccggaa ccctttcggc accgagaaac cgcttaaatc
    26281 ttgcattggt tttccttata ttcagacttt gttatataag gctttagtct tacctgtcgt
    26341 acgttggcgt aaatgccagg ataattctgc ccagaagcca ggtacatgta atatctgccg
    26401 ttggactttt ttggcgtcag cac
//
LOCUS       DAJTSA010000054        25824 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000054 DAJTSA010000000
VERSION     DAJTSA010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25824
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.054"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO369_002379"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO369_002379"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR5982631.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO369_002380"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO369_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR5982632.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO369_002381"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO369_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR5982633.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3807..3995
                     /locus_tag="OO369_002382"
     CDS             3807..3995
                     /locus_tag="OO369_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR5982634.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO369_002383"
     CDS             4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO369_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR5982635.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4756..5115
                     /locus_tag="OO369_002384"
     CDS             4756..5115
                     /locus_tag="OO369_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR5982636.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5112..7373
                     /gene="bcsA"
                     /locus_tag="OO369_002385"
                     /pseudo
     CDS             5112..7373
                     /gene="bcsA"
                     /locus_tag="OO369_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            7384..9723
                     /gene="bcsB"
                     /locus_tag="OO369_002386"
     CDS             7384..9723
                     /gene="bcsB"
                     /locus_tag="OO369_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR5982637.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO369_002387"
     CDS             9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO369_002387"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR5982638.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10913..11254
                     /locus_tag="OO369_002388"
     CDS             10913..11254
                     /locus_tag="OO369_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR5982639.1"
                     /translation="MDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRRSIDDIER
                     SLQNDRLAQQAEVLENQGKWAQAAALQRQRLALGPGSVWITYRLAGRSCAAIQRWRQQ
                     SGLRLHGAGIT"
     gene            11169..11420
                     /locus_tag="OO369_002389"
                     /pseudo
     CDS             11169..11420
                     /locus_tag="OO369_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCSC C-terminal domain-containing protein"
     gene            11502..13490
                     /gene="hmsP"
                     /locus_tag="OO369_002390"
     CDS             11502..13490
                     /gene="hmsP"
                     /locus_tag="OO369_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR5982640.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            13679..14965
                     /gene="dctA"
                     /locus_tag="OO369_002391"
     CDS             13679..14965
                     /gene="dctA"
                     /locus_tag="OO369_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR5982641.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            15186..16682
                     /locus_tag="OO369_002392"
     CDS             15186..16682
                     /locus_tag="OO369_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5982642.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(16778..17707)
                     /gene="kdgK"
                     /locus_tag="OO369_002393"
     CDS             complement(16778..17707)
                     /gene="kdgK"
                     /locus_tag="OO369_002393"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR5982643.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17939..18706
                     /gene="pdeH"
                     /locus_tag="OO369_002394"
     CDS             17939..18706
                     /gene="pdeH"
                     /locus_tag="OO369_002394"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR5982644.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18776..20827
                     /locus_tag="OO369_002395"
     CDS             18776..20827
                     /locus_tag="OO369_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5982645.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWSFRL
                     DNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFGLKA
                     QGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGGVDL
                     RLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGDSDI
                     HGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKSVQP
                     AGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQPLKF
                     GMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAELRG
                     SGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNCAAA
                     NLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSPLYV
                     RGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILSQMK
                     K"
     gene            complement(21009..22331)
                     /locus_tag="OO369_002396"
     CDS             complement(21009..22331)
                     /locus_tag="OO369_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR5982646.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22742..23755)
                     /gene="yhjD"
                     /locus_tag="OO369_002397"
     CDS             complement(22742..23755)
                     /gene="yhjD"
                     /locus_tag="OO369_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR5982647.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23804..24703)
                     /locus_tag="OO369_002398"
     CDS             complement(23804..24703)
                     /locus_tag="OO369_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5982648.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            25223..>25824
                     /locus_tag="OO369_002399"
     CDS             25223..>25824
                     /locus_tag="OO369_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5982649.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         6155 a         6487 c         7027 g         6155 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaaatc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag atgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1681 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaacaatga
     3061 aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tattttgcca
     3181 tgcgttattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggcc aaatagtata gacctttttc atgatttagc attgaaaata attttatttt
     3301 ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc
     3541 attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc attgaaatta
     3601 aaacttatca gatttagcat gtgaatcaat ttagaaggtc acaagacgtg tgattttagt
     3661 cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag caaattcccg
     3721 tctcgccgta aacaataaac cggcgaaatg ctcacggtta gaattgtctc atgaatggta
     3781 cggttatttc atagggatca agcaaaatga ataacaatga accagatact ctgcctgatc
     3841 ccgcgatagg ctatatcttc cagaatgata ttgtggcgtt aaagcaggca ttttcactgc
     3901 ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca ttaaaacgct
     3961 ggccgttgct ggcagagttt gcgcaacaaa agtaggggat tggtgaatgg ccgtactggg
     4021 attgcagggg gtgcggggag gcgtggggac aacaaccatc accgccgcat tagcctggtc
     4081 attacaaatg ttgggagaaa atgtcctggt ggtcgatgcc tgcccggaca acttgttgcg
     4141 cctgtcattt aatgttgatt ttacccaccg tcagggctgg gccagagcga tgctggatgg
     4201 ccaggactgg cgtgacgctg ggttgcgcta cacctcgcag ctcgatttgc tgccttttgg
     4261 tcagttatcc attgaagaac aagaaaatcc acagcactgg caaacccggc tgagcgatat
     4321 ttgctccggc ttacagcaac taaaagccag cgggcgttac cagtggattt taatcgactt
     4381 accgcgtgat gcctcgcaga taacccacca gctgctgagt ttgtgcgagc actcgctggc
     4441 aatcgtcaat gtggatgcca actgccatat ccgactgcat cagcaagcgc tgccggatgg
     4501 cgcacatatt ttgattaatg acttccgtat tggcagtcag gttcaggacg atatttacca
     4561 gctttggttg caaagccagc gccgattact gccgatgctc attcatcgtg atgaagcgat
     4621 ggctgaatgc ctggcggcta agcaaccagt aggtgaatat cgcagtgatg cgctggcggc
     4681 tgaagagata ctgacgctgg cgaactggtg cctgttgaac tactccgggc tgaaaacgcc
     4741 agtcgggagt gcatcatgag tatcctgacc cggtggttgc ttatcccgcc ggtcaacgcg
     4801 cggcttatcg ggcgttatcg cgattatcgt cgtcacggtg cgtcggcttt cagcgcgacg
     4861 ctcggctgtt tctggatgat cctggcctgg atttttattc cgctggagca cccgcgctgg
     4921 cagcgtattc gcgcagaaca taaaaacctg tatacgcata tcaacgcctc gcgtccgcgt
     4981 ccgctggacc cggtccgtta tctcattcaa acatgctggt tattgatcgg tgcatcgcgc
     5041 aaagaaacgc cgaaaccgcg cagcgggcat tttcaggtct gcaaaatatt cgtggacgtt
     5101 accatcaatg gatgaacgag ctgcctgagc gcgttagcca taaaacacag catctggatg
     5161 aaaaaaaaga gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg
     5221 tcaccttctc gctgattctg gcgttaatct gcgttactca gccgtttaac ccgctggcgc
     5281 agtttatctt cctgatgctg ctgtgggggg tagcgctgat cgtacggcgg atgccggggc
     5341 gcttctcggc gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt
     5401 ggcgttacac ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc
     5461 tgctcttcgc tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat
     5521 ggccgctgaa tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg
     5581 atatctttgt cccgacttac aacgaagatc tcaatgtggt gaaaaatacc atttacgcct
     5641 cgctgggtat cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca
     5701 gggaagagtt tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc
     5761 atgaacatgc gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg
     5821 tgtcgatttt cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct
     5881 ggttcctgaa agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg
     5941 acccgtttga acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct
     6001 atggtctggt gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctgtg
     6061 cggtgattcg tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg
     6121 aagatgcgca tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta
     6181 ttccgcaggc ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc
     6241 gctgggcgcg cgggatggta taaatcttcc gtctcgataa cccgctcacc ggtaaaggac
     6301 tgaagtttgc tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc
     6361 cacggctgat cttcctgact gcgccgctgg cgttcctgct gcctcatgcc tacatcatct
     6421 atgcgccagc gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga
     6481 ccaactcgaa gatccagggc aaatatcgcc actctttctg gagtgaaatc tacgaaacgg
     6541 tgctggcgtg gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca
     6601 aatttaacgt caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct
     6661 cgcggcccta catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc
     6721 gctacttcta tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt
     6781 tctacaacct gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac
     6841 gccgatcgaa ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc
     6901 tcttctcgtg taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc
     6961 aggcgcagat tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat
     7021 acgtcttccc gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc
     7081 cgctcaccac ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat
     7141 gggcgctctg gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc
     7201 tgaagctcgg cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg
     7261 gcatattccg tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccgcc
     7321 cggagcggag cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac
     7381 gcgatgaaaa gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc
     7441 tctttcatga cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta
     7501 gccgcccaga cggaacaaaa tccgcaggtg gggcaagtga tgtcaggcga gcagggagct
     7561 gatgccccca tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa
     7621 atcgcgccgc cgccgggcag catggtgcta cgtggtatta acccgaacgg cagcattgag
     7681 tttggtatgc gcagcgatga agtggtgacg aaggcgatgc tcaacttcga atacacgcca
     7741 tcgccatcgt tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg
     7801 ggcgtgctgc cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt
     7861 aatccactgt ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag
     7921 gacgtgtgcg aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga
     7981 ctggatctga cctatcagac cctgaatgtg aagaatgacc tgtcacactt cccggtgcca
     8041 ttctttgacc cgcgcgataa ccgtactaac accttgccga tggtctttgc gggtgcgccg
     8101 gatgttgggc tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc
     8161 tggcgtgggc agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc
     8221 tttgcaacca acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg
     8281 gtgattgaga tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt
     8341 cgtgacgaca aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc
     8401 cgtggtgaaa gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat
     8461 gcgccgaact gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa
     8521 gaacagttac aatccagcgg ccttgagcca gcagcgatta acgtttcgct aaacctgccg
     8581 ccggatctct acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc
     8641 atgccgccgg tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa
     8701 tccttcaacc tgagcagcaa acaggaggcg aaccgtctgc tgctgcgtat tccggtatta
     8761 caaggtttgc tggatggcaa aacagatgtc tctattccgg cgctgaaact gggcgcgacc
     8821 aaccagctgc gcttcgactt tgagtatatg aacccgatgc caggcggttc ggtagataac
     8881 tgtattacct tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac
     8941 ttctcgaagt attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc
     9001 ttcccattca gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaagcgcct
     9061 aacgaagcac agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc
     9121 ttcccggcga ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc
     9181 gacatcatga tcatcggtgg tatcccggac aaactgaaag acgataagca gatcgaccta
     9241 ttggtgcagg cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc
     9301 attgtgccgg acgagagcga tcgcgcggca gaaacccagt caacgctgac ctcttccggt
     9361 gcgatggcgg cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg
     9421 ctgttggcag atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc
     9481 aaacgcgcca ccatgttcgg ttcggtcgcg gtgatccgcg agtccggtat caacagccta
     9541 cgtgttggcg acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg
     9601 ctggcaaacc atccgattct gctggcggtg ctggcgacta tcagtgtgat attgctggca
     9661 tgggtactgt ggcgtctgct gcgaattatt agtcgtcgtc gtcttaaccc ggataacgag
     9721 taattgaaga tgaatgtgtt gcgtagtgga atcgtgacga tgctgttgtt ggctgccttt
     9781 agtgttcagg cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt
     9841 caggaagggc gcgtcatcga ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa
     9901 agttacggta tgttctttgc cctggcggct aacgaccgtg cagctttcga taatattctc
     9961 gactggacgc agaacaatct cgctcagggt tctttaaaag aacgtttgcc cgcctggctg
    10021 tggggcaaga aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt
    10081 gatgtctgga tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat
    10141 accgacatcg gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct
    10201 gggctgggtt ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt
    10261 tttaacccca gctacatgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg
    10321 tggaccacgc tgcgcgaaac caatcaacat ttattgctgg aaaccgcccc gaaaggcttt
    10381 tcgccagact gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca
    10441 ttgatcagca gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc
    10501 gatccgcaaa aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa
    10561 aacggttatc cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg
    10621 gtcggttttt ctgccgccat gctgcctttt ttacaaaacc gcgatgcgca ggcagttcag
    10681 cgccagcgcg tggccgataa ctttcccggc agcgatacct attacaacta tgtgctgacc
    10741 ctgtttggac aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta
    10801 cctgactggg gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc
    10861 tggccgtcat gccgatggtc gaggcagcac caaccatcag cagaccctgc ggatggacag
    10921 cggcaacact aatgccgtgc gcgggctggc gaatatttac cgccagcagt cgccagaaaa
    10981 agctgaagcg tttatcgcct cgctctctgc cagccagcgg cgcagcattg atgatatcga
    11041 acgcagcctg caaaacgacc gtctggcaca gcaggcagag gtactggaaa accagggcaa
    11101 atgggcgcag gcggcagcac ttcagcggca acgactggcg ctgggccccg gcagcgtatg
    11161 gattacttac cgactggcag gaagaagctg cgcggcaatc caacgatggc ggcagcagtc
    11221 agggcttcgg ctacacggcg cgggcattac ttgaacgacg tgttacttcc aactggtttg
    11281 ttggcacggc aattgatatc cagcaggtga aagattacgc acccagccat ttcctgctct
    11341 acgtacgtta ttccgccgcc ggatggcagg gtgacatgga tttaccgccg cagccgctga
    11401 taccttacgc cgactggtaa gttttcagat agcgcctctc ttaatgccgc tgcgatcggg
    11461 tatactcggg cggcaatctg ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt
    11521 taacaatcaa gcagatggca atggtggcag ccgttgtcct ggtgttcgtt tttatttttt
    11581 gcaccgtttt gctgttccat ctggtccagc agaatcgcta taacacggct acgcaactgg
    11641 aaagcattgc tcgctctgtc cgcgaaccct tatcttcagc tattttgaaa ggcgatattc
    11701 ccgaagcgga agctattctt gccagcatta aaccggcagg cgtggtcagc cgtgccgatg
    11761 tagtgctgcc taaccagttc caggcgctgc gtaaaagttt tattccagag cgtccggtgc
    11821 cggtaatggt tactcgcctg tttgagctac cggttcaaat ctcgctgggc gtctactcgc
    11881 tcgaacgtcc ggcaaatccg cagccaatag cctatctggt gctacaggcg gattccttcc
    11941 gtatgtataa gttcgtgatg agcactctct caacgttagt gaccatttac ttacttttgt
    12001 cgcttatcct gaccgtcgcc atcagctggt gcattaaccg cctgattttg catccgttac
    12061 gcaatattgc tcgcgaactt aacgccatcc cagcccagga gcttgttggt caccaactgg
    12121 cattaccgcg tctgcatcag gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca
    12181 accagcaatt gctgcagcgc cattatgaag aacagaacga aaatgcgatg cgcttcccgg
    12241 tgtcggattt gccgaacaaa gccttgctga tggagatgct ggagcaggtt gtcgcgcgta
    12301 aacaaaccac cgcgctgatg atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc
    12361 tgaaagaggc gcaacgagaa attctgctgc tgacgctggt ggaaaaactc aaatcggtac
    12421 tgtcgccacg tatgatcctc gcgcagatta gcggttatga ctttgctgtc attgccaacg
    12481 gtgtacagga accgtggcac gcaatcactt tgggtcagca agtactcact atcatgagcg
    12541 agcgcctgcc gattgaacgt attcaactcc gtccgcactg tagcattggc gtggcgatgt
    12601 tctacggcga tctcaccgcc gaacagcttt acagtcgcgc tatttctgcg gcatttaccg
    12661 ctcgccataa aggcaagaat cagattcagt tctttgatcc gcagcagatg gaagcagccc
    12721 agaagcggtt gacggaagag agcgatatcc ttaatgcact ggaaaatcat cagtttgcaa
    12781 tttggttaca gccacaggtc gagatgacca gcggtaaact ggtcagtgcg gaagtgttac
    12841 tgcgtatcca gcaaccggat ggcagttggg acctgccgga tggcttaatc gatcgcattg
    12901 agtgctgtgg gctgatggtt accgtcggtc actgggtgct ggaagagtcc tgtcgattgc
    12961 ttgcagcctg gcaagagcgc ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc
    13021 aactgatgca cccgaatatg gtggcggata tgctggaact gttaacccgc tatcgcattc
    13081 agccgggaac actgattctg gaagtgacag aaagccgacg tattgacgac cctcatgctg
    13141 cggtggcaat cctccgtccg ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg
    13201 gcatgggcta cgcagggctg cgtcagctgc agcatatgaa atcgttgcca atcgacgtac
    13261 tgaaaatcga caaaatgttt gttgaaggct tgccggaaga tagcagcatg attgctgcaa
    13321 ttatcatgct ggcgcagagc ctgaacttac aaatgattgc cgaaggcgtg gagactgaag
    13381 cacaacgaga ctggctggca aaagcgggcg ttggtattgc ccagggcttc ctttttgctc
    13441 gcccactccc tattgaaatc ttcgaagaga gttacctgga agaaaagtag ctaccccaaa
    13501 ctgattacaa aactttaaaa agtgctggtt tgtgcgagcc agctcaaact ttttaacctt
    13561 tttgtttcaa ttatgatcca ggtacatttc tgtgatgttg tctgggtgtt attttaaggc
    13621 cgcaggtacc ccccccccat aaccttacaa gacctgtggt tttactaaag gacaccctat
    13681 gaaaacctct ctgtttaaaa gcctttactt tcaggtcctg acagcgatag ccattggtat
    13741 tctccttggc catttctatc ctgaaatagg cgagcaaatg aaaccgcttg gcgacggctt
    13801 cgttaagctc attaagatga tcatcgctcc tgtcatcttt tgtaccgtcg taacgggcat
    13861 tgcgggcatg gaaagcatga aggcggtcgg tcgtaccggc gcagtcgcac tgctttactt
    13921 tgaaattgtc agtaccatcg cgctgattat tggtcttatc atcgttaacg tcgtgcagcc
    13981 tggtgccgga atgaacgtcg atccggcaac gcttgatgcg aaagcggtag cggtttacgc
    14041 cgatcaggcg aaagaccagg gcattgtcgc cttcattatg gatgtcatcc cggcgagcgt
    14101 cattggcgca tttgccagcg gtaacattct gcaggtgctg ctgtttgccg tactgtttgg
    14161 ttttgcgctc caccgtctgg gcagcaaagg ccaactgatt tttaacgtga tcgaaagttt
    14221 ctcgcaggtc atcttcggca tcatcaatat gatcatgcgt ctggcaccta ttggtgcgct
    14281 cggggcaatg gcgtttacca tcggtaaata cggcgtcggc acactggtgc aactggggca
    14341 gctgattatc tgtttctaca ttacctgtat cctgtttgtg gtgctggtat tgggttcaat
    14401 cgctaaagcg actggtttca gtatcttcaa atttatccgc tacatccgtg aagaactgct
    14461 gattgtactg gggacttcat cttccgagtc ggcgctgccg cgtatgctcg acaagatgga
    14521 gaaactcggc tgccgtaaat cggtggtggg gctggtcatc ccgacaggct actcgtttaa
    14581 ccttgatggc acatcgatat acctgacaat ggcggcggtg tttatcgccc aggccactaa
    14641 cagtcagatg gatatcgtcc accaaatcac gctgttaatc gtgttgctgc tttcttctaa
    14701 aggggcggca ggggtaacgg gtagtggctt tatcgtgctg gcggcgacgc tctctgcggt
    14761 gggccatttg ccggtagcgg gtctggcgct gatcctcggt atcgaccgct ttatgtcaga
    14821 agctcgtgcg ctaactaacc tggtcggtaa cggcgtagcg accattgtcg ttgctaagtg
    14881 ggtgaaagaa ctggaccaca aaaaactgga cgatgtgctg aataatcgtg cgccggatgg
    14941 caaaacgcac gaattatcct cttaatctca ccacttatgc ccgcactccc ttccccgagt
    15001 gcgggtgttt gcgcataatt gacccctgtg cctgaactta ccacctgggt gagttcaggt
    15061 ttatttcact tcttccgtga cattttcatg ttcttgcggt ctaacacgaa gtgtttttaa
    15121 cgtcatttag actgaccgac aacgggcagt ttttttatta ccaggatagt tgatcagggg
    15181 ttcacatgca gggcataaaa attcgacttt tagcgggcgg tttgctgatg atggccactg
    15241 ctggctatgt gcaggcagat gcgctccagc ctgatccagc atggcaacag gggacgcttt
    15301 ccaacggttt acagtggcaa gtgctgacca ccccccagcg tcccagcgat cgtgttgaaa
    15361 ttcgcctgct ggttaatacc ggttcgctcg ccgaaagtac acaacagagc ggttacagtc
    15421 acgccatccc tcgtattgcg ctaacgcaaa gcggtggcct tgacgcagca caggcgcgtt
    15481 cattgtggca gcaggggatc gaccctaaac gcccgatgcc gccggtaatt gtctcttatg
    15541 acaccacgct gtttaatctg agtttgccca ataaccgtaa cgatttgctg aaagaagcgc
    15601 tctcttatct ggcaaatgcc actggcaaat tgaccatcac accagaaacc atcaaccacg
    15661 cgctgcaaag tcaggacatg gtggcaacct ggcctgccga tactaaagag ggctggtggc
    15721 gctatcgtct gaaagggtca accttgttag gtcacgatcc tgccgatccg ctgaaacaac
    15781 ccgttgaagc ggaaaaaatt aaagatttct atcagaaatg gtacaccccg gatgcaatga
    15841 cgctactggt ggtgggaaac gtggatgcgc gctcggttgt cgaccaaatc aacaaaacgt
    15901 ttggcgaact gaaaggcaaa cgtgaaacgc cagctccggt gccgacgctt tctccgctgc
    15961 gtgcggaagc ggtgagtatt atgactgacg cggtgcgtca ggaccggtta tctatcatgt
    16021 gggatacgcc gtggcagccg attcgtgaat cagccgcact gctgcgctac tggcgtgcgg
    16081 acctggcccg cgaggcgctg ttctggcatg ttcagcaagc gttaagcgcc agtgacagca
    16141 aagacatcgg tcttggattt gactgccgtg tgctgtatct gcgtgcgcag tgtgccatca
    16201 acatcgaatc accaaacgac aagctgaaca gcaaccttaa tctggtggcg cgtgaactgg
    16261 cgaaggttcg cgataaaggt ctgccggaag aagagttcaa tgcgttagtg gcgcaaaaga
    16321 aactggagct gcagaaactg tttgccgcct atgcacgggc tgataccgat attctgatgg
    16381 gccagcggat gcgttcgttg caaaatcagg tcgtcgatat cgcgccggag cagtatcaga
    16441 aactgcggca ggatttcctt aacagcctga ctgtggagat gttaaatcag gatctgcgtc
    16501 agcagttgtc gaatgatatg gcgttaatac tgctgcagcc gaaaggcgag ccggaattta
    16561 acatgaaagc gttgcaggcg gcctgggatc aaatcatggc cccatctacc gccgctgcga
    16621 ccacctctgt cgccacggat gacgtacatc ctgaagtgac ggatattcca cctgcacagt
    16681 aatgcctgat ggtgatgagt tagcacaacc atcaggtatt tatggatgag ctggatagag
    16741 gggtaacact ttatccagcc ttttgcatat gctgcgttta cgctggcatc gcctcacgcg
    16801 ggataatcgc gccgcgatac tgaataacgg tacttgcggt caggtgcccg cgtttcgccg
    16861 cgttttccgc actgccgccc gtcaaacgta ccgccagata accggcgctg aaagagtcgc
    16921 cagctgcggt ggtatcgatc actttttctt tcggcagttt caccgccgga acatcaacta
    16981 acgcttcacc agcaatggac accaggcaag aatccgcccc gcgtttcacc accacttctt
    17041 tcacgcccgc gttatgggtg cgcgcaatga cgtcttccac cggctgttga ccccacagcg
    17101 cgtcttcgtc gtccagcgtc aggaaggcga tatccgtgca ttccagcatt tgctggtaca
    17161 cctgctgtgt ctcttctttg ctggcccaca ggcgcggacg atagttattg tcgaaaatca
    17221 cttttccgcc tttggcgcgg cattcgcgca gcagggaaag cagcttttcg cggctggtcg
    17281 ggcttaagat cgccaggcta atcccgctca ggtagagata atcgaaattc gccagctctt
    17341 cgcaaatcgc cgcagactgc tcgctcgcca gccagaattt ggcggcggct tcgttacgcc
    17401 agtagtagaa cgtacgttcg ccggtgctgt cggtttcaat gtagtaaagg cccggaagac
    17461 ggttttccat ccgttgggtc agggaagtat caacgttctc gccgtgccag gcgtccagca
    17521 tctgctggct aaaactgtcc gttcccagcg ccgttacgta atgaacggtt aatgccgcag
    17581 gatcgacctg acgggcgata tagacggaag tgttcagggt atcgccgccg aaaccgcgct
    17641 taacgtccgc gcctttctcg gaaagctcaa tcatgcattc gccaatcacg gcaatctttt
    17701 tggacatagt cgtgaacctg atctgtaaaa aattagcgtt agtttgcgct gtggtggttt
    17761 gctggtcaac catattaaaa cagtgttcca ttattttttt gagccagaac gtattcctga
    17821 aagattccgt tgtggagtga ggaaatttac cccggccagg cggtaaagtt ctgcccttac
    17881 gcgccgataa tctttgtcga gtccgggcag catcactttt aaacacagga catctttgat
    17941 gataaggcag gttatccagc gaataagcaa ccctgaagca agcatcgaga gcttgcagga
    18001 acggcgtttt tggttgcagt gtgagcgtgc ttacacctgg cagccgatct atcaaacatg
    18061 cgggcggtta atggccgtgg agctattaac ggtggtcacg catcccttga acccttcgca
    18121 acgcctgccg ccggatcgct attttactga aatcaccgtc agccatcgga tggaggttgt
    18181 gaaagagcag attgatttgc tggcgcaaaa agccgacttc tttgtagagc acggcctgct
    18241 ggcatcggtc aatattgatg gccctacgct catcgccctg cgtcagcaac caaaaatcct
    18301 gcgccagatt gagcgtcttc cctggctgcg tttcgaactg gtggagcata tccgtctgcc
    18361 gaaagattca acctttgcct cgatgtgtga atttggcccg ctgtggctgg atgattttgg
    18421 taccgggatg gcaaatttct ctgcactaag tgaagtgcgt tatgactaca tcaaaatcgc
    18481 gcgagaactg tttgtgatgc tgcgtcagtc gccggaaggc cgcacactct tttctcagct
    18541 tttacatcta atgaatcgct attgtcgcgg ggtgattgtc gagggcgtag aaacaccgga
    18601 agagtggcgt gatgttcaga actcgcccgc attcgccgca caaggctggt ttctttcacg
    18661 cccggcaccg atggaaacgc tgaatacggc ggttctggcg ctataagctg cctcattttc
    18721 cgccgggctg gactatcttt aggactggca caggaagaat atgaggaagc gaacgatgag
    18781 caaggcaggc aaaataaccg ctgcgatttc aggggctttc ttgttgttga ttgtcgtggc
    18841 gatcattttg attgcaactt ttgactggaa tcgactcaaa ccgaccatca accagaaagt
    18901 ctctgcggag ttgaatcgtc cgttcgctat ccgtggcaat ctgggcgtgg tgtgggagcg
    18961 gcaaaaacaa gaaactggct ggcgcagctg ggtgccgtgg cctcatgtac atgcagaaga
    19021 catcattctt ggtaatccac cggatattcc cgaagtcacg atggtgcatt tgccgcgagt
    19081 agaggcaacg ctggcgctgc tgaccaaaac ggtctggctg ccgtggatca agctcgaaaa
    19141 gcccgacgcg cgcctgattc gcctctctga aaagaacaat aactggacgt ttaatcttgc
    19201 caacgatgac aacaaagacg cgaatgcaaa gccgtcggca tggtcgtttc ggctggataa
    19261 tattcttttc gatcaagggc ggatcgccat tgatgacaaa gtaagcaaag cggatctgga
    19321 aatttttgtt gatccgttag gcaagccgct gccattcagc gaagttactg gatcgaaagg
    19381 taaagcggat aaagaaaagg tgggcgatta catttttggc ctgaaggcgc agggacgtta
    19441 taacggtgaa ccgctcacgg gtacgggaaa aataggcggt atgctggcgc tgcgtggcga
    19501 agggacgccg tttccggtac aggctgattt ccgttcaggt aatacccgtg ttgcttttga
    19561 tggcgtcgtg aatgacccaa tgaagatggg cggtgtcgat ttacggctta aattttctgg
    19621 cgattcactg ggtgatctct atgaactgac gggcgttctg ctgcccgata ccccgccgtt
    19681 tgaaacggat ggtcggctgg tagcgaaaat cgacactgaa aaatcgtcgg tctttgatta
    19741 tcgcggtttt aatgggcgaa ttggcgatag cgatatccac ggttctctgg tctacaccac
    19801 cggaaagcca cgaccaaaac tggaaggtga tgtcgagtcg cggcaattgc ggctggcgga
    19861 cctgggaccg ttgattggcg ttgattccgg gaaaggggca gaaaagtcga aacggtctga
    19921 acagaagaag ggcgaaaaaa gcgttcagcc tgcgggcaaa gtgctgcctt atgaccgctt
    19981 cgaaaccgat aaatgggacg ttatggatgc cgatgttcgc ttcaaagggc ggcgcattga
    20041 gcatggcagt agcctgccga ttagcgatct ttctactcat atcatcctca aaaatgctga
    20101 cctgcgcctg caaccgctga aatttggcat ggcgggcggc agcattgcgg cgaatattca
    20161 tctggaaggc gataaaaagc cgatgcaggg gcgggcagat attcaggctc gtcgactgaa
    20221 actgaaagaa ctgatgcccg atgtggaact gatgcagaag acgctggggg aaatgaacgg
    20281 tgacgcggaa ctacgcggta gcggtaactc ggtggcggcg cttttaggca acagtaacgg
    20341 caacctgaaa ctgttgatga atgacgggct ggtgagccgc aacctgatgg agattgttgg
    20401 gctgaatgtc ggcaactaca ttgtcggtgc gatatttggt gacgatgagg tgcgggtgaa
    20461 ctgcgcggcg gcgaatctga atattgccaa cggcgtggcg cgcccgcaga tttttgcttt
    20521 cgatactgag aacgcgttga ttaatgttac cggcacggca agttttgctt cggaacagct
    20581 ggatttgact attgatccgg agagtaaagg gattcggatt atcacactgc gttcgccgct
    20641 gtatgtgcgt gggacgttta aaaatccgca ggctggggtg aaagccggac cgctgattgc
    20701 ccgtggtgct gttgctgcgg cactggcaac gctggtaaca ccggcggcgg cgttactggc
    20761 actgatctca ccttccgaag gggaggctaa tcagtgtcgg acgatattgt cgcagatgaa
    20821 gaagtgatga taattgccgg atgtctgaca tccggcaatt aaacagattt agatttttgg
    20881 caaagtgtgc tttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaatcct
    20941 tgtgaattca atatattgta tggaaattca gtcctgataa gcgtagcgca tcaggctttt
    21001 cacacttatt acaacgactg atgtcgcgtc tcatgggtca gcagcagggc gattaacgtc
    21061 aagccagcca tcgccgccag atataacccc accgcaccta acccgtagtt agcctgcaac
    21121 caggctgcga tatacggcgc aacggaagcc ccgagaatcg acgatacgtt gtaagagaac
    21181 gatgctccgg tgtaacgcac ttctgtcgga aacagctctg gtaacagcgc acccatcggc
    21241 ccgaaggtca gccccatcag acttaacccc agcagcagga aggcaaaaac cagaatcggg
    21301 ttgccagaac cgagcagcgg gttaaaggcg aacagcgcga acaggatgat cagcgtggtg
    21361 atgatcacca tgcttttacg gcgaccaaag gcatcagcca gtaatccggc gactggcacc
    21421 atcacgccaa aaccaataac tgccatcatc agcatccaca acacttcgtt acgcggcagg
    21481 ccaagcccaa ctggcgcggc ggcggtactg aaggtcatag agtagaccgt catgatgtaa
    21541 aacagcgtat aggttgccag cataatgaac gtacccagta cggttacgcg aacatgtttg
    21601 gtcagcagcg tacccagcgg gatcttcacc tgttttttcg ctttagcgac tttctcaaac
    21661 accggcgact catgcagcga cacgcgaaca tacaggccga taatgaccag caccgccgag
    21721 aagataaatg gcacacgcca gccccagctc ataaactgct catcggtcag tagccaggaa
    21781 agcagcaaga aagtgccgtt ggcaaagaag aagccaatcg gtgcgcctag ctgtgggaag
    21841 gagccataca gtgcacgttt gcgcggtggg gcgttttcag tcgccagcag cgccgcgccg
    21901 ccccattcac cgcctaagcc cagaccctga ccaaatcgag ccagcgccag cagcagtggg
    21961 gcgaaaatac caatcgtggc atagcccggc agcagaccaa tcaccacggt cgaaatcccc
    22021 atcgttagca acgaggcgac cagcgtcgct ttacgcccaa cgcgatcgcc aaaatgacca
    22081 aaaacggcag agccaatggg gcgcgcgacg aaggcgatgg cgaaggtggc gagcgactgt
    22141 agcgttgctg ccgttggatc gccctgcggg aagaagatat gcggaaacac aataacggcc
    22201 gcagtggcgt aaatgtaaaa gtcgaagaac tcaatggccg tgccaatgag agaggcgaca
    22261 aggactttat tacgcgagtt gatcggcgtg tattcttgtt cgtggtcgag tgttgtggct
    22321 gttgcttgca taatcttttc ttattttttg gctaacgaat agccatatta cgcacagcaa
    22381 aagtgatatt tcaatgtgta acaaatcgcg gaaatggtca aaaaccaggc aaaaagggga
    22441 taatgtttag gccagtgatt tttcttctat gaaatgcttc acataatttt ataaatagtg
    22501 gataaaactg gtttcaggtt aaataaaagt taccaactgg atttatatgc tggggttatg
    22561 agttggagtg aaattgtttg ctcccaaata gatggctgaa gaggctctgg ggaagagttt
    22621 caagaggtta tgtgcgcatt atagccggat gcgatgctgg cgcatcttat ccggcctacg
    22681 gggagccagg ccgaataaaa tcgtatgccg gataaggcgt ttacgccgca tccggcctat
    22741 tttaaggctg cgttttccct ggcattcgcg ggtcgtcttt atattcggcg gtggcaatcc
    22801 acgccgcgca aaacagcgtc aaacgggcga agaagtagaa aaatgccatc agccccagca
    22861 cggaaccaaa tgctgcgcca gagggggatt tcatcagcga tggcagggtg taggtcatca
    22921 cgattttaat cacttcaaaa ccaatagcag ccagaaatgt tccgcgaatc agggcttttt
    22981 tgcgcggacg gtggcgcggc aaccgccaga agatccagaa gaaaagcaga tagttggcga
    23041 agatggaaat cgccaggcca atcaatcgcc acgtcggttt cagccactca atgctgttca
    23101 ggtgcagggc gctaataatc atttgctgcg ccgaaccggc aaccgaggtg atcgaaagcg
    23161 tcacaatcag cgcaatcaac aaaccaatca gtgaaataaa atcacgcaga tattttaccc
    23221 agaacttctc ctgatcttgc ggcgagcgtt cccagacatc gcgcgactgg gcacgaatcg
    23281 cttcacgcag gttacccatc cagttgatgc cggaataaag tgccaccgcc aggccgacaa
    23341 gccctacagt cgtacgctgc tgaacggcgg tgttgatggt gtttttcaac gtggcggcta
    23401 gtgtcggatc gctgatgttt tgcagaattt tgtcgaagat atcctgtagc aacatcggat
    23461 gggaggccag cacaaagccc cctgcggcaa acgacaccat caaaatcggg atcatcgaca
    23521 aaaatgagaa ataggtgata gccgcaccaa actggttacc cagccgatcg ttaaagcgtt
    23581 ctgtcgcgcg aataaggtgc gcaataaccg gttggcgttg gactttttcg gcagtggtgg
    23641 tgacggtttc cagcgcctgg ctaactttgc cgcctttctc gctattatcc aactgcttaa
    23701 tcggctcgtg ctccagatcc tgggtgggac gtttgatctc gttttcctgc gtcatccgct
    23761 cttttgtcct tctgtcgtgg tcattcttaa aaagtatagt cggtcagtcc aggtgctctt
    23821 tcattacgcc agccagccac tgcataaaca ggtttacacg ccgggaaagc tcccgacgct
    23881 gcgggtaaac cagcgaaagg gagagcggct cggcacggta gcctggcaac acttcaataa
    23941 gccgcccggc acgcagggct tcgcgcacgg caatgcgcgg gatctgaata atccccagac
    24001 cggcaagacc ggcggcgaga tagttttcgc tactgtttac cgtcaacatg ccgccagact
    24061 taaaccactg gacgccatta acgctggcaa cctcaaaacc taacggatgt acacccaggt
    24121 gcggtgtata acgcactatt gcgtgtgaag tcagatcatc ggggctttgc ggataaccaa
    24181 agcgcgtcag atagtgcgga ctggcacagt tgaccatggt tagtttgccg aggggacggg
    24241 cgataacgcc atcttccggt aacgcgccag tgcgtatcac gcaatcaaaa ccatcatgaa
    24301 gaatatctac cggacggtta tggctactca gttccagctc aattcccgga tgctgataga
    24361 gaaattccga cagacgcggc agtaacaggt ttttcgcgat tcctgacggg atgtcgatgc
    24421 gtaatttacc gctgatactg gtggcatcct gttgaaacag accgtccagt tcgctgagat
    24481 tactcaacac atcttttgcc cgttgataat aggtcatgcc ttctggcgtg agtttgaccc
    24541 gtcgcgtagt gcgctggaga agctgggtgc caagttgatg ttccagtgcc tgtatctggc
    24601 gcgaaacact tccctttggc aaagcaaaga aatccgccgc gcgggaaaaa ctttccagct
    24661 ccgcgacttt gatgaacaac tgcattgcgt gaattttatc catttctttg accgattgtt
    24721 gttcacagcg aaacagtaac gcgcatatcg cattgtttat tgttttttct aacatctaat
    24781 gccgggtttc acaatgttgt catgacgaat gtggcgattt ctcagcagga aaagggcgga
    24841 atagctttat gagaaaggtg aatcccctta aacagagcgt atttttcagt gattcccttt
    24901 aaagtgaata gaggacgtat tggctatttg tgcttatttt tgtctcattg ccatttgtta
    24961 ttttggcttg tagtcgcgga atttcatttt taattgcatg cgtactaacc accaatataa
    25021 ggaaatcctc aaaattgttt taaatagtgc cctcgacatg taatttcagg cgtttagctg
    25081 taactattta tatcggtaac tattttctac gcgtcatctc tgtagaatgg ccaccgccaa
    25141 atggcgaatt cttatttttt ctcgcgggta atacccttcc actacttaag tctttttaac
    25201 gtattcaggt ggagattcag ttatgcaaat agtcatgttt gacaggcagt caatatttat
    25261 tcatggaata aaaatcagtt tacagcagcg tattccagga gtgagtattc agggggtcag
    25321 tcaggcagac gagttatggc aaaagctgga aagttaccct gaagccttag ttatgctcga
    25381 tggcgatcag gatggtgagt tttgctactg gttgctgcaa aaaaccgtgg tgcagtttcc
    25441 tgaggttaag gtgttaatca cggcgacgga ttgcaataaa cggtggttac aggaagttat
    25501 ccattttaat gtgctggcca ttgtgcctcg cgattcaacc gtcgagacat ttgcgttggc
    25561 ggtaaatagt gcggcgatgg ggatgatgtt tctgcctgga gactggcgga cgacgccgga
    25621 aaaggacatt aaagatctca aatcgttaag cgcccgccag cgggagattt taaccatgtt
    25681 agcggcgggc gaatcaaata aggagatcgg cagagcgctg aatatcagta ccggaacggt
    25741 aaaagcgcat ctggaatccc tgtatcgccg tctggaagtg aaaaatcgca cccaggcggc
    25801 aatgatgtta aatatctcct cctg
//
LOCUS       DAJTSA010000055        25661 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000055 DAJTSA010000000
VERSION     DAJTSA010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25661
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.055"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1360)
                     /gene="yeaG"
                     /locus_tag="OO369_002400"
     CDS             complement(<1..1360)
                     /gene="yeaG"
                     /locus_tag="OO369_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR5982650.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQ"
     gene            1796..2542
                     /gene="mipA"
                     /locus_tag="OO369_002401"
     CDS             1796..2542
                     /gene="mipA"
                     /locus_tag="OO369_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR5982651.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2632..3393
                     /locus_tag="OO369_002402"
     CDS             2632..3393
                     /locus_tag="OO369_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR5982652.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3444..4328)
                     /locus_tag="OO369_002403"
     CDS             complement(3444..4328)
                     /locus_tag="OO369_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR5982653.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4412..5407)
                     /gene="gapA"
                     /locus_tag="OO369_002404"
     CDS             complement(4412..5407)
                     /gene="gapA"
                     /locus_tag="OO369_002404"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5982654.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5749..6162
                     /gene="msrB"
                     /locus_tag="OO369_002405"
     CDS             5749..6162
                     /gene="msrB"
                     /locus_tag="OO369_002405"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR5982655.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6204..6476
                     /locus_tag="OO369_002406"
     CDS             6204..6476
                     /locus_tag="OO369_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR5982656.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6846..7922
                     /locus_tag="OO369_002407"
     CDS             6846..7922
                     /locus_tag="OO369_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5982657.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7949..9328
                     /locus_tag="OO369_002408"
     CDS             7949..9328
                     /locus_tag="OO369_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5982658.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9345..10390
                     /locus_tag="OO369_002409"
                     /pseudo
     CDS             9345..10390
                     /locus_tag="OO369_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10411..11247
                     /locus_tag="OO369_002410"
     CDS             10411..11247
                     /locus_tag="OO369_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR5982659.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11252..12199
                     /locus_tag="OO369_002411"
     CDS             11252..12199
                     /locus_tag="OO369_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCR5982660.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12209..13189
                     /gene="ydjG"
                     /locus_tag="OO369_002412"
     CDS             12209..13189
                     /gene="ydjG"
                     /locus_tag="OO369_002412"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR5982661.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13326..14084
                     /locus_tag="OO369_002413"
     CDS             13326..14084
                     /locus_tag="OO369_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5982662.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14202..15560
                     /locus_tag="OO369_002414"
     CDS             14202..15560
                     /locus_tag="OO369_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5982663.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15653..16294)
                     /gene="pncA"
                     /locus_tag="OO369_002415"
     CDS             complement(15653..16294)
                     /gene="pncA"
                     /locus_tag="OO369_002415"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR5982664.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVGVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16305..17321)
                     /gene="ansA"
                     /locus_tag="OO369_002416"
     CDS             complement(16305..17321)
                     /gene="ansA"
                     /locus_tag="OO369_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR5982665.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17488..19344)
                     /gene="sppA"
                     /locus_tag="OO369_002417"
     CDS             complement(17488..19344)
                     /gene="sppA"
                     /locus_tag="OO369_002417"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR5982666.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19505..20056
                     /locus_tag="OO369_002418"
     CDS             19505..20056
                     /locus_tag="OO369_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR5982667.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20173..21216
                     /gene="selD"
                     /locus_tag="OO369_002419"
     CDS             20173..21216
                     /gene="selD"
                     /locus_tag="OO369_002419"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR5982668.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21221..23182
                     /gene="topB"
                     /locus_tag="OO369_002420"
     CDS             21221..23182
                     /gene="topB"
                     /locus_tag="OO369_002420"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR5982669.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23309..24349
                     /locus_tag="OO369_002421"
     CDS             23309..24349
                     /locus_tag="OO369_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5982670.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24466..>25661)
                     /gene="gdhA"
                     /locus_tag="OO369_002422"
     CDS             complement(24466..>25661)
                     /gene="gdhA"
                     /locus_tag="OO369_002422"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR5982671.1"
                     /translation="LERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGG
                     MRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMT
                     ELYRHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEA
                     TGYGLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTV
                     VDESGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPCATQNE
                     LDVDAAHQLIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEM
                     AQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAML
                     AQGVI"
BASE COUNT         6516 a         5973 c         6625 g         6547 t
ORIGIN      
        1 tctgctgttc caggacgtag aacagatgga ccgggtttgc cgccacttct acatgatcga
       61 agttgaacac gcgggagagg atcttaaacg caaaacgcgt cgacagaccg ttcatccctt
      121 catcgacacc ggcgtagtca cgatattcct gatacgactt ggctttcgga tcggtgtctt
      181 tcagactttc gccatcataa acccgcatct ttgaataaat gctggagttt tctggctctt
      241 tcaggcgcga aagaatggaa aaacgtgaca gtgtttcgag cgtgccaggg gcgcatgggg
      301 cgtgagtcaa ttcactgtga ttaagcaatt tctcgtagat tttgatctct tcggaaatgc
      361 gcaagcaata cggcaccttc acgatgtaaa cacggtcgag gaaggcttcg ttgtttttgt
      421 tattacggaa agtgacccat tcggacttgt tcgagtgggc gagaataatc ccgttgaacg
      481 gcagggcgga gataccttcc gtcccgttgt agttaccttc ctgagtggcg gttaacaagg
      541 gatgcagcac ttttatcggt gctttaaaca tctcaacgaa ttccatgatc ccctgattgg
      601 cgcggcacag cgcaccggaa tagccgtagg cgtccgggtc attctgcgcg tagtgttcga
      661 gtttacgaat atcgactttc ccaaccagcg cggagatgtc ctggttgttc tcatcaccgg
      721 gttccgtttt ggcgatagca atttgttgca gaattgacgg ccagaccttc actacccgga
      781 acttagtgat atcgccacca aattcatgca ggcgttttgc cgcccatggc gacatgatgg
      841 tgccgagata acggcgagga atgccatact ctttttccag aatctgagca tcttcctgcg
      901 gattgaaaag acagaacgga tgatcgttga ccgggctacg ctcaccgttg gcgctcaata
      961 cataaatcgg tacgagctgc attaatgatt tcagtcgctc agcaagcgat gatttacccc
     1021 cacccacagg ccccagcaga tacaggattt gtttcttctc ttccagcccc tgagccgcgt
     1081 gtttcagata agagacaatc tgttcaatcg cgtcttccat gccgtaaaac tcttcaaatg
     1141 ccggataacg tgcaatgacc cggttagaaa agagtcgaga aagtctgggg tcctgggctg
     1201 tatcgaccat gacaggctca ccgatagcca tcaatagccg ctcagccgcg ttggcataag
     1261 cactgcgatc ttgccgacaa gtggtaagaa actcctgcag tgtgaactct tcgtccttgg
     1321 cagcttcata tcgctggcga tagtgatcga atatattcat ggtatgccgt cctttcgttt
     1381 tttagcacag gttaagagcc gttcatatga atagtagagg ctcccggaag aggtaaactg
     1441 aacgacgtct cttgctaaaa cacagcaact ctcatgccat tttatcgacg atgagaacaa
     1501 cgtgcccggt gatacacctt caaaattaag cgtagatggc aaatggaaaa cttgcctgca
     1561 cggctttctt aatttcaatg gcatatcaac aactcatcca taaattttgc ataattaatg
     1621 taaagaccag gctcgccagt aacgctaaat ccatttggct gtaagcgcgg tgtcatccgc
     1681 ggcaggaaaa ttaaacagtt actttaaaaa atgaaaacgt aaaaaggttg ggtttcgatg
     1741 tattgacggg taaactttgt cgcccgctaa acatttgttt aaggaatgat taattgtgac
     1801 caaactcaaa cttctggcac ttggagtgct tatcgcaacg tctgcaggcg tagcgcacgc
     1861 tgaaggtaaa ttttccctgg gcgcaggcgt aggtgtcgtt gagcacccat ataaagatta
     1921 cgataccgat gtttacccag taccggtaat caactatgaa ggcgataact tctggttccg
     1981 tggcttaggt ggtggttact acctgtggaa tgacgcaacg gataaacttt caattaccgc
     2041 ttactggtcg ccgctttact tcaaagcgaa agacagtggc gatcaccaaa tgcgtcacct
     2101 ggatgaccgt aagagcacca tgatggctgg tctgtcttat gctcacttta cccagtacgg
     2161 ttacctgcgt accaccctgg ctggcgatac cctggataac agcaacggca ttgtctggga
     2221 tatggcctgg ttgtatcgtt acaccaacgg tggcctgacc gtgactccgg gtattggtgt
     2281 gcagtggaac agcgaaaacc agaacgaata ctattatggc gtatcgcgca aagagtccgc
     2341 tcgcagcggt ctgcgtggct ataacccgaa tgacagctgg agcccttacc ttgagctgag
     2401 cgccagctac aacttcctcg gcgactggag tgtttacggt accgcgcgct acacccgtct
     2461 gtctgatgaa gttactgaca gcccgatggt ggataaatcc tggactggcc tgatttctac
     2521 cgggatcacc tacaaattct gataatgcat ctttccaggg cattttgtgc ataaaaacag
     2581 ggcgtcacgc cctgttttgc attacggtgg tgcaatacct gggaggaaac aatgcaacaa
     2641 aaaatgattc aatttagtgg cgatgtctca ctgccagcca tagggcaggg aacatggtat
     2701 atgggcgaag atgccagtca gcgcaaaaca gaagttgctg cactacgcgc aggcattgaa
     2761 ctcggtttaa ccctcattga taccgccgaa atgtatgccg atggcggtgc cgaaaaggtg
     2821 gttggggaag cattaaccgg tctgcgagag aaggtctttc tcgtctctaa agtctatccg
     2881 tggaatgctg gcgggcaaaa agcgataaat gcatgcgaag ccagtttacg ccgtctcaat
     2941 actgactatc tcgatctcta cttattacac tggtctggca gtttcgcttt tgaagagact
     3001 gttgctgcga tggaaaaatt gatcgcccag ggaaaaatcc gtcgctgggg cgtttctaac
     3061 cttggttatg ccgatatgca ggaactctgg cagctgccgg ggggaaatca gtgtgccact
     3121 aatcaggtgc tttaccatct cggttcacga gggattgagt acgatctact cccctggtgc
     3181 cagcaacagc agatgccggt gatggcttac agtccattag cccaggccgg gcggttgcgc
     3241 aatggactgt taaaaaacgc ggtagtcaac gaaattgcac atgtccaaca acatgcagct
     3301 gcgcttgagg tcgaactttc gtcagcggaa ttagctatgc tggataaggc atatccggca
     3361 ccaaaaggaa aaactgcgct ggatatggtg tgatgttgat acgtgccgga tgcatcgcac
     3421 ccggcacaca acattaacgc aaattaacgt ttggcaacgc gaatggattg cgccagatgt
     3481 gcaggtttct ctttggtgac tttttgcgtt tctgaagcgt aagccgtttc tacacaaaca
     3541 aaggtcttgt agccatcgtc cggcatgtcg cccatgctaa ctgaaagcgc cggtcccggg
     3601 ttccagccga caacgttcag atgatgctgg tggcctacgg cgataatacg attcagcgct
     3661 tcatcattaa tcacgctgca atcttgcgga ttcagataca cgcgatcggt acggtcaggg
     3721 aaggtctgaa taccgtcggt cagtacatct tctttcgcgt cattcacttt atcaatgaag
     3781 cgatcgccca gcccactgac gcttacctta gcgatatcac ccacgttaaa gtaggtatgc
     3841 agggcagagg tggtttcaaa ttcgccatgt gattcaagat cgatttcaca ggttttaccc
     3901 acgcggaaat gcgctaacag cgtaaagtcg tgcggccaga attttttcgt ctcttcgctt
     3961 tgtgtcaatt caaaagtcag cgctacgcca tcagcatctt catgatgtga tttcagcgtc
     4021 cacggcaggt tgcgggcaaa accgtgcgca ggcagacctt gttgtgccgc cggaccaaac
     4081 cacggccagc aaaccggtac gccaccgcga atagcgacgc catttttgaa cggtgtgttg
     4141 ttgctcaacc acagaacttc ttcttcaccc gcaggtttcc acgagagaag gtgtgcgccc
     4201 tgtaatgcaa aagaggcttt tacctgggga tgatcgacca caatgaggtc cagttcatcc
     4261 agtttacgac gggagaggac aggggagatt tgttcgatga ccggaagggc aaaaattttc
     4321 ttaatcatga cgcagtcctt taacttcatt ttatcaggta aaaaaaagag cgaccgaagt
     4381 cgctcttttt agatcacagt gtcatctcaa cttatttgga gatgtgagcg atcaggtcca
     4441 gaactttgtt ggagtaaccg gtttcgttgt cgtaccagga taccagtttc acgaagttgt
     4501 cgttcagagc gataccagct ttagcatcga acacggaagt gcaaacttcg ccgttgaaat
     4561 cggtagatac tacgtcatct tcggtgtagc ccagaacgcc tttcatttcg ccttcagcag
     4621 cagctttaac ggcagctttg atctgctcgt aagttgcagc tttttccaga cgaacggtca
     4681 ggtcaactac ggatacgttc ggggtcggaa cgcggaacgc cataccagtc agtttgccat
     4741 tcagttctgg cagtacttta cctacagctt tagcagcacc ggtagaggac gggatgatgt
     4801 tctgggaagc gccgcggccg ccgcgccagt ctttgtgaga cgggccatca acggttttct
     4861 gagtagcggt agtagcgtga acggtggtca tcagaccttc gatgatgccg aagttatcgt
     4921 tgataacttt agccagcgga gccaggcagt tggtggtgca ggaagcgttg gaaacgatgt
     4981 cctggccagc atatttgtcg aagttagcgc ctttaacgaa catcggagtg ttgtctttag
     5041 acggaccagt cataaccact ttcttcgcac cagcggtgat gtgtttacga gcagtttcgt
     5101 cagtcaggaa cagaccagtt gcttcagcga caacgtcaac acccacttcg tcccatttca
     5161 ggttagccgg atcacgttca gcggtaacac ggattttttt accgttaacg atcagatgac
     5221 cgtctttcac ttcaacggta ccgtcgaaac ggccgtgagt ggagtcatat ttcagcatgt
     5281 atgccatgta atcagcgtct aacaggtcgt tgattgcaac gatctcgatg tcagaacgtt
     5341 tctgagcagc acggaaaaca atgcgaccga tacggccaaa accgttgata cctactttga
     5401 tagtcatata ttccaccagc tatttgttag tgaataaaag gttgcctgta aaattacaaa
     5461 aaccttacgc agcgtcaagc ggaatcgtgt caatcattgc gacaaatcaa tcctgtgcct
     5521 aagcattacg cgactgactc gcctcacttt tcctttgggc ttgagaccac atggggtcgg
     5581 cgccccgaat tttaaagggc aattacgata aaaatgtgat tttcatcacg atttaggtgc
     5641 agccatttcg tcgagatcaa gtttaggaca aaattttgcc agttggtgtt aatgttttgt
     5701 tagaatcagt cacgtaatgt gagcacgatt aaagtgagat gtgagtaaat ggcgaataaa
     5761 ccttcggcag aagaactgaa aaaaaatttg tccgagatgc agttttacgt gacgcagaat
     5821 catgggacag aaccgccatt tacgggtcgt ttactgcata acaagcgtga cggcgtatat
     5881 cactgtttga tctgcgatgc cccgctgttt cattccgaaa ccaagtatga ttccggctgt
     5941 ggctggccca gtttctacga accggtgagt gaagaatcca ttcgttatat caaagacttg
     6001 tcacatggaa tgcagcgcat agaaattcgt tgcggtaact gtgatgccca tctggggcat
     6061 gtctttcccg acgggccgca accaacaggc gaacgttatt gtgttaactc tgcttcttta
     6121 cgcttcactg atggcgaaaa cggcgaagaa atcaacggtt gaaaaaacga ttcagcaact
     6181 tattccacag gagcggaaaa attatgaatc ttgatgacat tatcaacagc atgacgcctg
     6241 aggtatacca gcgtttgtcg accgccgttg aactggggaa atggcctgat ggcgttgcgt
     6301 tgaccgagga acaaaaagag aattgcctgc aactggtgat gctgtggcaa gcccgccata
     6361 ataccgaagc acagcatatg acaattgaca caaatggtca gatggtcatg aagagcaaac
     6421 agcagttaaa agaagatttt ggtatctcag caaagcctat tgccatgttt aagtaagtgg
     6481 ttcatcccct ttttgccgcg gctatgaagg ggatagccaa caatgacccc acgactcgtc
     6541 tcaattatct cgacccgacc tggctaagtc ccagtccgat ctcttgcagt cgcgttcacg
     6601 cctgaatgac ttctcctgaa cttaagtctc agacctattt ggccggtaat ccctctcgaa
     6661 tttgctcttt ttgcgtcccg ccttccacca acctgtaaca gacccagcca ttttctgcgt
     6721 aattgtgatt tgagttgtaa aatgcaggtc attatctgtg ttgtgcagat ttaatattga
     6781 cctcaaaaat catgtgggta agtatgatgg ccatctaagc aaaacaaaac acagaggatg
     6841 acgcaatgaa agcactggct cggtttggca aggcctttgg cggctacaag atgattcatg
     6901 tcccacaacc catttgtggc ccggaagacg tagtgactga aattaaagcc gcggcaatct
     6961 gcggcgcaga catgaagcac tacaatgtcg atagcggttc tgatgagttt aactctatcc
     7021 gcggccatga gttcgcaggt tgtattgcgc aggttggtga aaaagtcaaa gactggaaag
     7081 tggggcaacg cgtcgtgtcg gataacagcg gtcacgtttg cggcgtttgt ccggcctgtg
     7141 aacagggtga ttttctgtgt tgtacagaaa aagtaaacct tggtctggat aacaatacct
     7201 ggggcggtgg tttttccaaa tattgtctgg ttcctggtga aattctcaaa attcatcgtc
     7261 atgcgttgtg ggaaatccct gatggtgttg attatgagga cgcagccgta cttgacccta
     7321 tctgtaatgc ctataaatcc atcgcgcagc aatcgaaatt ccttcctggt caggatgttg
     7381 tcgtcattgg cactggccca ctcgggctgt tttccgtaca aatggcgcgg attatggggg
     7441 cggtaaatat cgtcgtcgtt ggtctgcaag aagatgtggc ggtccgcttc ccggttgcaa
     7501 aagaactggg ggcgacggca gtagtaaatg gttctaccga agatgtggtg gcgcgctgcc
     7561 agcaaatttg tggcaaagat aatctgggac tggtgattga atgctccggt gccaatatcg
     7621 cactgaaaca agccatcgaa atgctccgcc cgaatgggga agtggtacgc gttggaatgg
     7681 gcttcaaacc tcttgatttc tcgattaatg acattaccgc ctggaataaa agcatcattg
     7741 ggcatatggc ctatgactcc acctcatggc gtaacgctat ccggctatta gccagcggcg
     7801 ctatcaaagt caaaccgatg atcacgcatc gtatcggcct gtcgcaatgg cgcgaagggt
     7861 ttgatgcgat ggtcgataaa accgcaatca aagtgatcat gacttacgac tttgatgaat
     7921 aactactctc ctgaaggaac gttatcgcat ggaacagata acaaaaccgc attgtggtgc
     7981 acggctggat cgcttaccgg attgccgctg gcattcgtca atgtttgcca tcgtcgcgtt
     8041 tggcttgctt gtctgctgga gtaatgccgt tggtggcttg atcctcgcgc agctgaaagc
     8101 gttgggctgg acagataatt ccactacagc cacattctca gcaatcacga ccgccggaat
     8161 gtttctcggt gcgctggtgg gcggcatcat tggcgacaaa accggtcgca gaaatgcgtt
     8221 catcctctat gaggccattc atattgcctc gatggttgtc ggtgctttct caccgaatat
     8281 ggattttctt attgcctgcc gttttgtgat ggacgttggg ctgggagctt tactggttac
     8341 actgtttgct ggtttcaccg aatatatgcc cggtagaaat cgcggaacgt ggtccagtcg
     8401 ggtttccttt attggcaact ggtcatatcc gctctgctca ctgattgcga tgggactcac
     8461 gccgctgatt agtgcagagt ggaactggcg ggtacaactg cttattcctg caatattgtc
     8521 gcttatcgct acggcgctgg cctggcgcta ctttcctgaa tccccgcgct ggctggaatc
     8581 gcgcggacgg tatcaggaag ccgagaaagt catgcggagt atagaagaag gcgtcatacg
     8641 ccagacgggt aaacctttgc cgcctgtggt tattgctgat gacggtaaag cgccacaagc
     8701 ggtgccgtat tcagccttac tgacaggagt attactgaaa cgcgtgatat taggctcttg
     8761 tgtgctgatt accatgaacg ttgtgcagta cacactaatt aactggttgc caacaatatt
     8821 catgacccag gggattaatt taaaagactc gattgtttta aataccatga gtatgtttgg
     8881 cgcgccattt ggtattttta ttgccatgct ggtgatggat aaaattccgc gtaaaacaat
     8941 gggtgtgggg ctattaatcc tgattgcggt gctcggatat atctattcac tgcaaaccag
     9001 tatgttgctc ataacgctga ttggtttctt cctgattact ttcgtctata tgtacgtttg
     9061 ctatgcctcg gcagtgtatg tccctgaaat ctggccgaca gaggccaaac tccgtggctc
     9121 cggtctggcg aatgcggtag ggcgaatcag tggtattgcc gcaccttatg ccgttgcagt
     9181 gctgctcagt agttatggtg taacgggagt ctttattctt ctgggggctg tttcaaatat
     9241 tgtcgcaatt gctatcgcca ccattggaat tgaaaccaaa ggtgtctccg ttgaaagttt
     9301 aagtattgat gcagtagcca ataaataata cgggtggatt caaaatgaaa aattcaaaag
     9361 caatattgca ggtgccgggc acaatgaaaa ttatttcagc agaaatacca gtgcctaaag
     9421 aagatgaagt tttgattaaa gtagaatatg tcggtatttg tggttcagat gtacatggtt
     9481 ttgaatcagg cccgtttatt ccgcctaaag acccaaatca agaaattggc ctgggtcatg
     9541 aatgcgccgg gacggttgtg gctgtgggaa gccgcgtgcg caaatttaaa ccgggggatc
     9601 gggtaaatat cgaacctggc gttccttgcg gtcactgtcg ttactgtctg gaaggcaaat
     9661 ataacatctg cccggacgtt gattttatgg cgacacaacc caactaccgc ggcgcattaa
     9721 cgcactatct gtgtcatccg gagagcttta cttacaaact gcccgacaat atggacacga
     9781 tggaaggggc gctggtggag cctgccgcag tcgggatgca tgccgcgatg ctggcagatg
     9841 ttaaaccggg taagaagata attattctgg gagcaggttg tattggtttg atgacgttgc
     9901 aagcgtgcaa atgcctgggc gcaacggaaa ttgccgtcgt tgatgtactg gaaaaacgtc
     9961 tggcaatagg ggaacagcct ggtgcgacag tggttattaa tggcgcaaaa gaggacacta
    10021 ttgcacgctg tcagcaattt accgaagaca tgggcgcaga tattgttttc gaaacagcgg
    10081 gttctgcggt caccgttaaa caggcacctt taatctggta atgcgcggcg gtaaaattat
    10141 gattgttggt actgtacccg gcgattcggc aatcaatttc ctcaaaatca atcgcgaagt
    10201 cactatccag acggtattcc gctatgccaa tcgttatccg gtcacgattg aagctatttc
    10261 ttcagggcga ttcgatgtga aatcgatggt gacgcatatt tacgattatc gggatgtaca
    10321 acaggcattt gaagagtcag ttaacaacaa acgcgacatt attaaaggcg ttattaaaat
    10381 tagcgattaa ttaatacggg agaacagaac atgctcgcag atatcagata ttgggaaaac
    10441 gatgccacaa ataaatatta tgcaattgcc cattttaacg tatggaatgc agaaatgttg
    10501 atgggcgtta aagacgcagc cgaagaagcg aaatccccgg ttattatttc ttttagtaca
    10561 ggttttgtcg gtaacacctc atttgaagat ttctctcaca tgatggtatc aatggcacaa
    10621 aaagcaacgg tgccggtaat aactcattgg gatcatggtc ggagtattga gattattcat
    10681 aacgcctgga ctcatggtat gaattcatta atgcgtgatg cttctgcatt tgatttcgaa
    10741 gaaaatattc gtttaaccaa agaggctgtt gatttctttc atccgctggg tattccggta
    10801 gaggcggaat tagggcatgt cggtaatgaa accgtttatg aggaagcgct ggcgggctat
    10861 cactataccg atcctgacca ggcggctgaa tttgttgaac gtacgggctg tgactcattg
    10921 gctgtcgcca tcggcaacca gcatggggtt tatacgtcag agccgcaatt gaactttgag
    10981 gtcgtcaaac gcgtacgcga tgctgtttct gttccgctgg ttttgcatgg cgcatcgggg
    11041 atcagtgatg ccgacattaa aactgcaatt tcgttgggta ttgcgaaaat caacattcat
    11101 acggagctct gtcaggccgc aatggtagca gtgaaagaaa accaggatca gcctttcctg
    11161 catttagaac gtgaagtacg taaagctgta aaagaacggg cattggaaaa aattgaactg
    11221 ttcggttctg atggcaaagc ggaataatga tatggataat ctcgacgtta tttgtatagg
    11281 tgccgctatt gttgatattc cattacaacc ggtcagtaaa aatatctttg atgtagattc
    11341 ttatcctctt gaaagaatcg caatgagcac cgggggcgat gcaataaatg aagcaacaat
    11401 tatttctcgt ctggggcatc gtaccgcatt aatgagccgc attggtaaag atgcctcagg
    11461 gcaatttatt ctcgaccatt gtcgcaaaga gaatattgat attcaaagcc tgaaacagga
    11521 cgtcagcata gatacatcta ttaacgttgg actggtgacg gaggatggcg agcggacgtt
    11581 tgtcacgaac cgtaacggca gtttgtggaa attaaatatt gacgatgttg attttgcgcg
    11641 gtttcctcag gcgaaattat tatcactggc cagtattttc aacagtcctc tattggatgg
    11701 taaagcgcta acagaaattt ttacgcaagc gaaagcccgg cagatgatca tttgtgccga
    11761 tatgatcaaa ccgcggttga atgaaacgct ggatgatatt tgcgaagcat tgagttatgt
    11821 cgattatctg tttccaaatt ttgccgagga aaaattactc acagggaaag agacactgga
    11881 tgaaattgct gactgctttc ttgcgtgcgg cgtaaaaacg gtggtgatta aaacgggtaa
    11941 agacggctgc tttatcaagc gtggtgacat gacgatgaag gtgccggcgg tcgcaggaat
    12001 aaccgccatc gacaccattg gcgcgggcga taactttgct tcaggtttta ttgcggcact
    12061 gttagaaggc aaaaatctgc gtgaatgcgc acgctttgcc aatgcaacgg cggctatctc
    12121 ggttctaagc gtcggtgcca ccaccggcgt aaaaaacaga aagctggtcg aacaattgct
    12181 ggaagaatac gaaggataat gaaggcaaat gaaaaagata cctttaggca cgacggatat
    12241 aacgctttcg cgaatggggt tggggacatg ggccattggc ggcggtcctg catggaatgg
    12301 cgatctcgat cggcaaatat gtattgatac gattcttgaa gcccatcgtt gtggcattaa
    12361 tctgattgat actgcgctag gatataactt tggcaatagt gaagttatcg tcggtcaggc
    12421 gttaaaaaaa ctgccccgtg aacaggttgt agtagaaacc aaatgcggca ttgtctggga
    12481 acgaaaagga agtttattca acaaagttgg cgatcggcag ttgtataaaa acctttcccc
    12541 ggaatctatc cgcgaagagg tagaagccag cttgcaacgt ctgggtattg attacatcga
    12601 tatctatatg acgcactggc agtcggtgcc gccatttttt acgccgatag ctgaaactgt
    12661 cgcagtgctt aatgagttaa aggctgaagg gaaaattcgc gctataggcg ctgctaacgt
    12721 cgatgctgac catatccgcg agtatctgca atatggtgaa ctggatatta ttcaggcgaa
    12781 atacagtatt ctcgaccggg caatggaaaa cgaactgctg ccgctatgtc gtgataatgg
    12841 cattgtggtt caggtttatt ccccgctaga gcagggattg ttgaccggca ctatcactcg
    12901 tgattacgtt ccgggcggcg ctcgggcaaa taaagtctgg ttccagcgtg aaaacatgct
    12961 gaaagtgatt gatatgcttg aacagtggca gccactttgt gctcgttatc agtgcacaat
    13021 tcccactctg gcactggcgt ggatattaaa acagagtgat ttaatctcca ttcttagtgg
    13081 ggctactgca ccggaacagg tacgcgaaaa tgtcgcggca ctgaatatca acttatcgga
    13141 tgcagacgca acattgatga gggaaatggc agaggccctg gagcgttaaa tactcaacac
    13201 gattcacctc tgtttctgtg aaaactgtga tgaaaatctg tgattcaaat aggttatttt
    13261 gaaagtaaac atcggttatg cgataatcgc gctaatgtga tgtaaaaaga tacaggggtg
    13321 taatcgtggc ggcaaaagac aggattcagg caattaagca aatggttgcc aacgataaaa
    13381 aggtgacagt ctcaaatttg agtgggattt ttcaggtaac cgaagaaacc attcgccgcg
    13441 atcttgagaa gctggaagat gaaggctttt tgaccagaac ctatggtggt gctgttttaa
    13501 atacagcggt gttgacggag aatatccatt tttataagcg cgcttcatcg ttttatgaag
    13561 agaagctgct tattgcacgc aaggcactac cctttatcga caataaaacc accatggcag
    13621 ccgattccag tagtaccgta atggaattgc tcaaattgtt acaggaccgt agtgacctga
    13681 cgttgctaac caactcggcg gaagcgattc atgttctggc tcagtcagaa attaaagtcg
    13741 tttcaacggg tggggaacta aacaaaaata cactttccct gcaaggaaga attactaaag
    13801 agatcatcag ccgctaccat gtcgatatca tggtaatgag ttgtaaaggt cttgatatta
    13861 acagcggcgc gctggactct aatgaagcgg aagctgaaat caaaaagaca atgatccgtc
    13921 aggcgacaga agttgcgtta ttggttgatc actctaagtt tgatcgtaaa gcttttgtcc
    13981 agttagctga ctttagtcat attaattata taataactga taaatcaccg ggtgcagagt
    14041 ggatagcatt ttgcaaagac aataatatcc agctggtgtg gtaatttaag cacacgttgt
    14101 caatattatt tgttcctgcg actggaaata aaatgatgat aaagatgcgg caagtgaatt
    14161 tgccgcaatt gctacctttt atagcaaatc cgaggcaccc aatggaacaa tatgatcaaa
    14221 ttggcgcaag actggaccgc ttgcctttgg cccggtttca ttatcgtata tttggtataa
    14281 taagctttag tctgttatta acggggtttt tgagttactc aggtaatgtc gtcttagcaa
    14341 agctggtaag caatggatgg tcaaataatt tcctctatgc cgcctttacc tcggcattaa
    14401 tgtttggtta tttcatcggc tcacttactg gtgggtttat tggtgactac tttgggcggc
    14461 gcagggcgtt tcgcataaat cttctcatcg tcggtattgc tgcaacaggg gccgcttttg
    14521 tccctgatat gtactggctc attttctttc gcttcctgat gggaacagga atgggggcgc
    14581 tgattatggt tggctatgcc tcatttacgg agtttatccc cgcgacggtg cgtggaaaat
    14641 ggtccgcgcg gctctcattt gttggtaact ggtcgcccat gctgtctgcg gcgataggcg
    14701 tggtggttat cgcttttttt agttggcgaa taatgtttct tctgggtggt attggcatac
    14761 tgttagcctg gtttctctca ggtaaatact ttatcgagtc gccacgatgg ctggaaggga
    14821 aagggcaaat cgccggtgca gaaagccaac ttcgtgaagt agagcagcaa attgaaaggg
    14881 agaagagtat tcgtttaccc cagcttactt tgaaccagag caacagcaag gttaaggtaa
    14941 tcaagggtac cttcaggctc ctgtttaaag gggaaatgtt acgacgtaca ttagttgcga
    15001 taactgtttt aattgcaatg aacatttcgc tttataccat taccgtatgg ataccgacca
    15061 tatttgttaa ctccggcatt gatgtcgata aatcaatatt aatgaccgct gttattatga
    15121 ttggtgctcc ggtaggaata tttattgcgg cattaattat tgatcatttt cctcgtcgat
    15181 tatttggctc cgccttactt attattattg ccgtgttagg ctatatctat tcaagtcaga
    15241 ctacagagtg ggcgatttta atctatggtc tggtgatgat cttcttttta tacatgtatg
    15301 tttgcttcgc gtcggcggtt tatatcccgg agctttggcc aacgcattta cgcctgcgcg
    15361 gttcgggttt cgttaatgcc gtcggacgga tcgtcgcagt tttcacgccc tatggcgttg
    15421 cggcattatt aacacattat gggtcgatca cggtgtttat ggtgcttggt gtcatgttag
    15481 tgctctgtgc gctggttctc tccatttttg gcatcgaaac gcggaaggtg tcgctggaag
    15541 agatttctga ggtgaattaa atctttcgtt gatggcatca attgccagat gtaccactga
    15601 cgtgttttat ttggcctaca atgccagtac agaattgtag gccagcgtaa aattacccct
    15661 gcgtctcttc ccagtctgcc agcgtatata gcgttgcccc agctgctgac atctccataa
    15721 acgcgtgtgc actgtcctgg ggctggatat tcacgccacg acagccatcg gtaatcacgt
    15781 ttaccttata acctaactgt aacgcgtcca gcacggtaaa cttcacgcaa tagtcagtag
    15841 ccaggcccat aacgatcaat tcatcgattt catgatcgcg caaccagtca tcaagcgcgg
    15901 ttttctgccg acgtccgtta tcaaaaaagg cactgtaact gtcaactaaa ggattttcgc
    15961 ctttatggaa caccgctgcg atctcttttt gattcagtaa cggatgtaat tgtgcgcctt
    16021 cactgttctg cacacagtgt tcaggccaga aagtttgccg caggccgccg agttggcctg
    16081 gcgtataagg ctctacgccg tgctggctgg caaagctgcc gtgattcgcc ggatgccagt
    16141 cctggctggc gataaccgct tctccgcgcg actggcacca gtcaataagg cggttagcaa
    16201 cccccaccgt actgtcacct tccggtacgg cgagcgcgcc accagcacag aaatcatttt
    16261 gtaaatcgac cagtaacagg gcgcgatggg gcattgcagt ctccttaatc atccggcgtc
    16321 agttcgccgc gcaggttttg gctcatggcc ttgcgaatgg tttcagtatc cagttcctgg
    16381 ctcagcaggt aatgcagttt ggttagcgtg gcttctacag tcatatctgc accgtcaata
    16441 acgccggcat gggcgagggc gttaccggtg gcataaccac tcatattcac tttaccggac
    16501 atacattgtg tcaggttgac caccacaata ccgcgatcgc tggcttcttg taattcctgc
    16561 aggaaggctt tgttttgtgg cgcattaccc acgccatagg agcgcagaat caatgctttc
    16621 accggttggc gcagaaaatt gcgcacgacg tcagcagaaa tccctggata aatcgtcacc
    16681 acgccaattg gttgtggcgt gattggatga acgattaatt ccccttcacc gtgcggggcg
    16741 ggtggcgtat tcaaacgacg aatatggata ccagcttcca gtaacggagg aaggtttgga
    16801 gaggcaaacg catcaaaacc atcggcatgg gctttggtag tgcggttgcc gcgatacaat
    16861 cggttattga aaaagagcgt tacttcgttg atcggataat tcggcgcaac gtacaacgca
    16921 ttcagcagat taatttgtcc gtcagagcgt aactcagcca gcgggatttg tgaccctgtc
    16981 acaatgaccg gtttaccgag attctcgagc atgaacgaca gcgcagaggc ggtatacgcc
    17041 atcgtgtcgg taccgtgtag aatgacaaaa ccatcatagt cgtcatagtg cgctttaata
    17101 tcttcagcaa tatgctgcca gtcttccggc gtcatatcag aagaatccat caacggcgta
    17161 tattcatgaa tggtgaaatc tggcatctcc gggcgatgga attccggcat cagcgccagt
    17221 tggcgttgta gatgacctga caccggaata taaccctgct cggaacgctg catcccgatg
    17281 gtcccgcccg tgtaggcgac gtaaattgat ttcttttgca tgatattgat atgaatgata
    17341 tcgaaagagc aaaagtatat acgtgaggca ggaggagcgc acctgatact atgcggataa
    17401 ggaaatatgg ggcgagacag cgggaagagg attcgtcgag gtaaaaaata atgtggccga
    17461 cgatctgtcg gccactcagt acaagactta acgcacgttg gcgcaggtca ggcaaaacgc
    17521 ataacggttt tgtgggtcgt taaacgcggc cagcttgtcg ctttcacttt taacggtaga
    17581 ggctaccgag gcaagcggtg caggtaacat ggcctggaac gcatctggca acattgcccg
    17641 gacagaacca gacatgttgt ccatcacttt gtcgaagaag gtcggttcat caacgtagta
    17701 ttccagatgc cactgtttca cttttgcgag ctctgctgct ttggcaaccg catcatcgaa
    17761 atccccgaga ctatcgacca gcccgttagc ttttgcatcc tgaccggtcc agacgtggcc
    17821 ctgggcgatt ttatcaattt gctccggcgt cgaatgacgc gcatcagcaa ccagcgtgat
    17881 aaagcgttta tagccattct caatgcttaa ttgcatcatc tgctgcgctt ccggcggcag
    17941 tgccctggtg atagaaacat ccgccagcgg tgaagttgag acaccatcag tatgaacacc
    18001 aatcgaatcc agactatttt ctacggtggt gatcacgccg aagataccga tagaaccggt
    18061 cagggtgctg gggttagcca caatgtaatt agctggcgtg gaaatccagt aaccaccaga
    18121 tgccgccatg ccgcccatcg atacaaccac agacttaccc gctgcccggg ctgctgccag
    18181 ttcagcgcga atcacttcag acgcggtaac gctgccgcct gggctattaa cacgcaggac
    18241 aatcgctttc actttcgggt caaggcgagc gtcgcggatt tgtgccgcag tggtatcacc
    18301 gccaacattc ccctgagttt cctcgccatc cataattgcg ccattagcga agacgacacc
    18361 gatgctgtca ccggtatctg ccggcgtttt caatgcgtaa tcgtaataac tgatggcgcg
    18421 ataattttta tcagtcttac tccagccgaa ctctttggtc agagcttttt cgatttcggc
    18481 actcgatgcc agtgcatcga ccagcttgtt ttccagtgca tatttcgcgg tatcgccacc
    18541 ggttttggtt aaaccctcaa gcaacccttg cgcgccaggg aatacctgct gagcagggat
    18601 ctgccggtta gcggcaacag tattcagata gttttgccac agctcaccaa tccagcggct
    18661 gtcagcttcg cgggctgccg gtgacatatc atcacgaata aacggttcaa cggcagattt
    18721 atacgtaccc acgcggaaca catgggtgga aactttcagc ttatccagca acgatttgta
    18781 gtacagaccg ttggtagcaa aaccgtgcag atcaaccacg ccttgcggtg acagccaaat
    18841 tttattggcg aaactggcga gataatattg cccctggctg tagttctcgc caacggcata
    18901 aaccggtttc ccgctgtcac gaaactcttt cagggctttg ccgatgtact gcatagacgg
    18961 ctggtcgccg cctgcgaagt ttttcagatc catcacgata ccggtgatat tgcggtcgtc
    19021 cttcgcctgg cgaatggtgt tgacgatatc aaacagtgag ttttcctgca gacgatcgga
    19081 actggcacca agcagctggc ggcttaattt actaaaccgc tgagaactgt ctggtttatc
    19141 gacgatcaca ccagaaatgt ccagcagcag tgcgccacga ctggccgttt ctttcgaatc
    19201 accaccactg acctgcatcc agatccccac accaaccagt acgaggaaaa taaagaacag
    19261 gttaagtacc atttcacgga cgaaattcag cagacgccac gtccatttaa aaaatccggc
    19321 aataaatcgc caaagggttc gcatgtattc tcccaactta aggtcacacc tgtcacaggc
    19381 gcaatagatg tggttatcgt aatgacccaa ccggcacttg tcagcaggaa ttgcggactg
    19441 tgctgtaaca aaatccactc tcgtgttaat tttgtgaata aatatcacga caggagttaa
    19501 tcaaatggat gcactcgaac tattgatcaa tcgccgtagc gcctcccgcc tggctgaacc
    19561 cgcgccaacg ggtgaacaac tgcaaaacat cctgcgtgcg ggtatgcgtg cgccggacca
    19621 taagtccatg caaccgtggc atttttttgt gattgaaggg gaagggtgcg agcgtttcag
    19681 cgccgtactg gaacaggggg cgattgctgc cggtagtgat gataaagcta tcgacaaagc
    19741 ccgtaatgcg ccgttccgcg caccgctcat catcacggtg gtggcgaaat gcaaagaaaa
    19801 tcataaagtc ccgcgatggg aacaggaaat gtctgccgga tgcgcggtca tggcgatgca
    19861 aatggcagcc gttgcccagg gatttggcgg catctggcgc agtggcgcat taactgaaag
    19921 tccggtagtg cgtgaagcat tcggttgccg tgagcaggat aaaattgtcg gttttctcta
    19981 cctcggcacg ccgcagttga aagcatctac gtcgattaac gtcccggacc cgacgccgtt
    20041 tgtaacttat ttctgataat tgtcgcgaaa ctgtctggat tgtgagcaaa ggcgcaggaa
    20101 ttcagacact ctcacttatc acttcacgga atgagggtta ccatacccgg attgcattga
    20161 caggagatgt ccatgagcga gaactcgatt cgtttgaccc aatacagcca cggagctggt
    20221 tgcggctgta aaatttcccc aaaagtgttg gaaaccatcc tgcacagtga gcaggcgaag
    20281 tttgttgatc cgaatttgct tgtgggtaat gaaacccgcg acgatgcggc ggtgtacgat
    20341 ctgggcaatg gcaccagcgt tatcagtacc accgacttct ttatgccgat cgttgataat
    20401 cctttcgatt ttggccgcat tgcggcgact aacgccatca gcgatatctt cgcgatgggg
    20461 ggcaaaccga ttatggcgat tgcgatcctc ggctggccga ttaacaaact ttccccggaa
    20521 attgcccgcg aagtaaccga aggtggacgc tatgcatgcc gtcaggcggg tattgcgctg
    20581 gctggcggtc actccatcga tgcgccggag ccgatttttg gtctggcggt aacggggatc
    20641 gtaccgaccg agcgggtgaa gaaaaacagt accgcacaag ccggatgcaa actgttcctg
    20701 acgaaaccgc tggggatcgg cgttcttacc acggctgaga aaaaatcact gttgaaacca
    20761 gaacatcagg gactggcgac ggaagtgatg tgccggatga acatcgcagg cgcgtccttt
    20821 gccaacatcg aaggcgtaaa agcgatgacc gacgttacgg gctttggtct gctgggccac
    20881 ttgagcgaaa tgtgtcaggg ggctggtgtg caggcacgcg tcgactatga agcgatcccg
    20941 aaactccccg gtgttgaaga gtacattaag ttgggcgcag tacctggcgg cactgaacgt
    21001 aactttgcca gctacggtca tctgatgggt gaaatgccgc gtgaagtgcg cgatctgctg
    21061 tgcgatccgc aaacttctgg cggtttgctg ctggcggtca tgccggaagc agaaaatgag
    21121 gttaaaacta cagccgccga gtttggcatt gaactgacgg cgattggcga actggtgcca
    21181 gcgcgcggcg gtcgtgccat ggttgagatc cgttaattta atgcggttgt ttattgccga
    21241 aaaaccgagt ctggcgcgcg ccattgctga tgtcctgccc aaaccgcacc ggaaaggcga
    21301 tggctttatc gagtgcggta atggtcaggt ggtgacttgg tgtatcggtc acctgcttga
    21361 gcaggcgcag ccagacgcct acgacagccg ctatgcgcgc tggaatcttg cggatttgcc
    21421 gattgtcccg gaaaagtggc aattacagcc acgaccctcc gtgaccaaac aacttaacgt
    21481 catcaaacgc ttcctgcatg aagccagcga aatcgttcac gccggggacc cggatcgtga
    21541 agggcaattg ctggtggatg aagtgctgga ctatctgcaa ctggcaccgg aaaagcgcca
    21601 gcaggtgcag cgttgcttga taaacgacct gaacccgcag gcggttgagc gggcgatcga
    21661 ccgtcttcgt tccaacagtg agtttgtgcc attgtgcgtt tctgcgctgg cgcgagcgcg
    21721 tgccgactgg ctgtacggca tcaatatgac ccgtgcgtat accattctcg gtcgcaatgc
    21781 cggttatcag ggcgtacttt ccgtgggacg cgtgcagaca cccgtgctcg ggctggtggt
    21841 gcgccgcgat gaagagattg aaaacttcgt ggcgaaagac ttctttgaag tcaaagcaca
    21901 tatcgtgaca cctgccgatg agcggtttac cgctatctgg caaccgagcg aagcgtgtga
    21961 accgtaccag gatgaagaag ggcgcttgtt acatcgtcca ctggcggagc atgtggttaa
    22021 ccgcattagt ggtcaaccgg ctattgtcac cagctataac gataaacggg aatcagaatc
    22081 cgcgccgctg ccgttttcgc tttcggcgtt gcagattgaa gcggcaaaac gcttcggtct
    22141 gagcgcgcag aacgtgcttg atatctgcca gaagctgtac gaaacgcaca agctaatcac
    22201 ttatccgcgt tccgattgtc gctatttgcc agaagaacat tttgccggac gccacgcggt
    22261 gatgaatgcc atcagcgttc atgcaccgga tctgttgccg cagccagtgg tagatccaga
    22321 tatacgcaac cgctgttggg atgacaaaaa ggtcgatgcg caccacgcca tcatcccgac
    22381 cgcacggagt tctgcgatca acctgacgga gaacgaagcg aaggtctata acctgattgc
    22441 ccgtcaatac ctgatgcagt tctgcccgga tgcggtgttc cgcaagtgtg ttatcgaact
    22501 ggacattgcc aaaggcaaat ttgtcgctaa agcccgtttt cttgctgaag caggctggcg
    22561 cacgctgtta ggcagcaaag agcgcgatga agaaaacgac ggcacgccat tgcctgtggt
    22621 ggcgaaaggc gatgagttgc tgtgtgaaaa aggtgaagtg gtagagcggc aaacccagcc
    22681 gccgcgccat tttaccgatg caacactgct ttcggcgatg accgggatcg cgcgctttgt
    22741 gcaggacaaa gatctgaaaa aggtccttcg tgcgaccgat ggtctgggga cagaagcaac
    22801 ccgtgccggg attattgaac tgctgttcaa gcgtggtttt ctgaccaaaa aagggcgcta
    22861 tatccactcc accgacgccg gaaaagcgtt attccattcg ctgccagaaa tggcgacgcg
    22921 accggacatg accgcgcact gggaatcggt gctgacgcaa atcagcgaaa agcagtgtcg
    22981 ctatcaggac tttatgcagc cgctggtggg gacgctatat cagcttattg atcaagccaa
    23041 acgtacgccg gtgcggcagt ttcgcggcat tgtggctccg ggcagtggtg gcagtgctga
    23101 taagaaaaag gctgcaccgc gtaaacgtag tgcgaaaaaa agtccgccag cagatgaagc
    23161 cggaagcggg gcgatagcgt aagcgagcga atctttcgtg ctattcgagt catattctga
    23221 aatatccagc ggatcaagaa agttcgttgg atattttttt gcatggataa aattatcgcc
    23281 tctaaagtat gtaataacag ggaatgtgat gaaaaaagtt ttattgcaaa accatcctgg
    23341 gagcgagaag tattctttta atggctgggg aatatttaat agtaattttg aacgggtgat
    23401 taaagaaaat aaggccatgc tgctttgtaa gtgggggttt tatttaacat gtgttgtcgc
    23461 tgtaatgttt gtattcgcag cgataacatc caacggtttg aatgaaagag gcctgattac
    23521 cgcgggatgc tcttttcttt atctattaat tatgatgggg cttattgttc gggccggttt
    23581 taaagcaaaa aaagaacaac tgcattatta tcaggctaaa ggtattgagc cgctcagtat
    23641 cgagaagtta caggcgctac aattgatcgc accttatcga ttctatcata agcaatggtc
    23701 tgaaacgcta gagttctggc cgcgaaagcc tgaacctggc aaagatacct tccaatatca
    23761 tgtgcttcct tttgactcga tcgatatcat cagtaaaaga cgcgagtctt tagagaatca
    23821 atggggtatc gaagatagcg aaagttattg tgccttaatg gagcattttc tttctggcga
    23881 ccatggagcc aataccttta aagcaaacat ggaggaagcc ccagagcagg ttatcgcctt
    23941 gttgaataaa tttgctgttt ttccctcaga ctatatctct gattgcgcta ataatcgctc
    24001 cggtaaatcc tcggcgaagc taatatgggc ggcggaatta tcatggatga tctcgatatc
    24061 aagcacagct tttcaaaacg ggacaattga agaagaactg gcctggcatt atataatgct
    24121 tgcttctcga aaggcgcacg agttgttcga aagcgaagaa gattatcaaa aaaatagtct
    24181 aatgggattt ctttactggc atatctgctg ctatcgcaga aagttaacgg atgctgaact
    24241 tgaagcgtgt tatcgttacg acaagcagtt ttgggagcac tacagtaaga aatgccgttg
    24301 gcccataaga aatgttccct ggggagcatc atccgttaaa tactcataaa cgcctgaaat
    24361 tttgccgggg gcgctgcgct tgaccggcct acattagagc gtaactgcaa tgaattgtgc
    24421 ccatctgtag gcctgataag cgtagcgcca tcaggcattt acaacttaaa tcacaccctg
    24481 cgccagcatc gcatcggcaa ccttcacaaa accggcaatg ttcgcgccct gcacgtagtt
    24541 ggtttgctca ccttcaccac catgctcaac acaggcatgg tggatatcca gcatgatgtg
    24601 atgcaaacgt gcgtcaactt tctcggcttt ccagcccagg cgcgcagcgt tttgtgccat
    24661 ttccaggccc gacgttgcta caccgccagc attagccgct ttacccggtg caaacagtac
    24721 gcctgcctgc tggaacagtt cagtcgcttc gatggtggtc ggcatatttg ccccttcagc
    24781 gacggcttta acgccattgg cgataagctg atgcgcggcg tcaacatcca gttcgttctg
    24841 ggtggcgcaa ggcagggcga tatcaaccgg tacagaccac ggctgttggc cttcgagata
    24901 gaccagacca aattctttgg cgtaatctgc cgctcgacca tcgcggctgg ctttgatttc
    24961 gataagacgt gccagtttct ctttcgtgaa tccgctttca tcaactacag tgccgctgga
    25021 gtctgacgca gtgatcacac gagcaccaaa ttccatcgct ttttcgatag cgtactgggc
    25081 gacgttgccg gagccagaaa cggaaacgct catcccttca aaacccatac cgtggcgttt
    25141 taacattgct tctgtgaaat aaaccagacc gtagccggta gcttccgggc gaataagact
    25201 accgccaaat gaaaggccct taccggtgaa gacgcaggcg gtattgttgg agagcttttt
    25261 catcatcccc gccataaagc cgacttcacg accaccaacg ccgatatcac ctgccggaac
    25321 gtcggtatcc gcgcccaggt ggcgatacag ttcagtcatc agcgtctggc aaaaacgcat
    25381 cactttacct tcgctttttc ctttcggatc gaaatcgctg ccgcctttac caccgcccat
    25441 cggcagggta gtcagggcat ttttgaaggt ttgttcaaag ccgaggaatt tgagaatgga
    25501 aaggttaact gacggatgga agcgcatacc gcctttgtac gggccgatgg cagagctgaa
    25561 ctgcacacgc catgcacggt tgacctgtat ctggttgcga tcatcaaccc ataccacgcg
    25621 aaactggatc acgcgctccg gttcaaccag acgctccagt a
//
LOCUS       DAJTSA010000056        24186 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000056 DAJTSA010000000
VERSION     DAJTSA010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.056"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(25..597)
                     /locus_tag="OO369_002423"
     CDS             complement(25..597)
                     /locus_tag="OO369_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR5982672.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            815..1675
                     /gene="tesB"
                     /locus_tag="OO369_002424"
     CDS             815..1675
                     /gene="tesB"
                     /locus_tag="OO369_002424"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR5982673.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="OO369_002425"
     CDS             complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="OO369_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR5982674.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="OO369_002426"
     CDS             complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="OO369_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR5982675.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3559..5340)
                     /locus_tag="OO369_002427"
     CDS             complement(3559..5340)
                     /locus_tag="OO369_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5982676.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5333..7105)
                     /locus_tag="OO369_002428"
     CDS             complement(5333..7105)
                     /locus_tag="OO369_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5982677.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7135..7593)
                     /gene="decR"
                     /locus_tag="OO369_002429"
     CDS             complement(7135..7593)
                     /gene="decR"
                     /locus_tag="OO369_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR5982678.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7747..8565)
                     /gene="cof"
                     /locus_tag="OO369_002430"
     CDS             complement(7747..8565)
                     /gene="cof"
                     /locus_tag="OO369_002430"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR5982679.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8665..10357
                     /locus_tag="OO369_002431"
                     /pseudo
     CDS             8665..10357
                     /locus_tag="OO369_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10422..11117
                     /gene="queC"
                     /locus_tag="OO369_002432"
     CDS             10422..11117
                     /gene="queC"
                     /locus_tag="OO369_002432"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR5982680.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11169..11567)
                     /gene="fadM"
                     /locus_tag="OO369_002433"
     CDS             complement(11169..11567)
                     /gene="fadM"
                     /locus_tag="OO369_002433"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR5982681.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11661..12032)
                     /locus_tag="OO369_002434"
     CDS             complement(11661..12032)
                     /locus_tag="OO369_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR5982682.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12183..14054)
                     /gene="ppiD"
                     /locus_tag="OO369_002435"
     CDS             complement(12183..14054)
                     /gene="ppiD"
                     /locus_tag="OO369_002435"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5982683.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14246..14518)
                     /gene="hupB"
                     /locus_tag="OO369_002436"
     CDS             complement(14246..14518)
                     /gene="hupB"
                     /locus_tag="OO369_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR5982684.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14727..17081)
                     /gene="lon"
                     /locus_tag="OO369_002437"
     CDS             complement(14727..17081)
                     /gene="lon"
                     /locus_tag="OO369_002437"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR5982685.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17269..18543)
                     /gene="clpX"
                     /locus_tag="OO369_002438"
     CDS             complement(17269..18543)
                     /gene="clpX"
                     /locus_tag="OO369_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR5982686.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18669..19292)
                     /gene="clpP"
                     /locus_tag="OO369_002439"
     CDS             complement(18669..19292)
                     /gene="clpP"
                     /locus_tag="OO369_002439"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR5982687.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19539..20837)
                     /gene="tig"
                     /locus_tag="OO369_002440"
     CDS             complement(19539..20837)
                     /gene="tig"
                     /locus_tag="OO369_002440"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR5982688.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21181..21498)
                     /gene="bolA"
                     /locus_tag="OO369_002441"
     CDS             complement(21181..21498)
                     /gene="bolA"
                     /locus_tag="OO369_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR5982689.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21803..22381
                     /locus_tag="OO369_002442"
     CDS             21803..22381
                     /locus_tag="OO369_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5982690.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22425..23900
                     /gene="ampG"
                     /locus_tag="OO369_002443"
     CDS             22425..23900
                     /gene="ampG"
                     /locus_tag="OO369_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR5982691.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5666 a         6666 c         5829 g         6025 t
ORIGIN      
        1 ccgggcggag agggttaacg tcgcttaata ttgcgtagga gcgggaactg ccgaagacgg
       61 attcacctga gttggtgaag tcgaaggtac ggtagtcgtt gcgccaccgc tggcctgaac
      121 cggcacggcg gtttgctgta ccggcaccaa tgtcaggtcg gctttagttc cgccctggtt
      181 gatcaccggc tgaacggtat cggtgataaa taccagtttg tcattcacgg taatcgccgc
      241 actcaacaga atacgcgcgt tcggctgaac atctgccggg ttaaatggca gaacaaagct
      301 gaatggtgac tgtttacctt cagtacgcac cgctttctgc gccagcactt ttgacggtgc
      361 atcggctaac gacgcgtcag aaagtgtcac ggtcagcaca gcatcaggcg gcagtgcgac
      421 tttctgacgg atccagacag taccggagac attcggttgc tggatagcag gttgttgtgt
      481 tgctgaaata gacgtatttg cagccggggc tggcgtctga atatccgcgc ttttatctgc
      541 gcaagccgcc aacgcaatcg caaccgctaa accactggcc atgtgcacga gtttcattca
      601 tttctcctta ttatcaatgc accagcgggc taactttcct cgccggaaga gtggttaaca
      661 aaatagtaac gtcaacaagt gtggcacaca tcacgcattt ctgcctgtaa ttagcccgta
      721 attcagacta tttcacccat cggaccagtg gcaaatttgc gttatactca actcactttg
      781 gcttgctgcg gcagctttgt tactggagag ttatatgagt caggcgctaa aaaatttact
      841 gacattgtta aatctggaaa aaattgagga aggactcttt cgcggccaga gtgaagattt
      901 aggtttacgc caggtgtttg gcggccaggt cgtgggtcag gccttgtatg ctgcaaaaga
      961 gaccgtcccg gaagagcggc tggtacattc gtttcacagc tactttcttc gcccaggcga
     1021 tagtaagaaa ccgattattt atgatgtcga aacgctgcgt gacggtaaca gcttcagcgc
     1081 ccgccgggtt gctgctattc aaaacggcaa accgattttt tatatgacgg cctctttcca
     1141 ggcaccagaa gcgggtttcg aacatcaaaa aacaatgccg tccgcgccag cgcctgatgg
     1201 cctgccttcg gaaacgcaaa tcgcccaatc gctggcgcac ctgctgccgc cagtgcttaa
     1261 agataaattc atctgcgatc gtccgctgga agtccgtccg gtggagtttc ataacccact
     1321 gaaaggtcac gtcgcagaac cacatcgtca ggtgtggatc cgcgcaaatg gtagcgtgcc
     1381 ggatgacctg cgcgttcatc agtatctgct cggttacgct tctgatctta acttcctgcc
     1441 ggtagctcta cagccgcact gcatcggttt tctcgaaccg gggattcaga ttgccaccat
     1501 tgaccattcc atgtggttcc atcgcccgtt taatttgaat gaatggctgc tgtatagcgt
     1561 ggagagcacc tcggcgtcca gcgcacgcgg ctttgtgcgc ggtgagtttt atacccaaga
     1621 cggcgtgctg gttgcctcga ccgttcagga aggggtaatg cgtaatcaca attaaaaaaa
     1681 tagccggagg tgaaaaccgt ccggctattt tttgcagtgc ttgttacgcg ttataggcat
     1741 tctcgccgtg gctgttgacg tccagccctt ctcgctcttg ttcttccggc acacgtaggc
     1801 caaccgtcag atccgccagt ttgtagccaa taaatgccac cacaccggac cagacgatcg
     1861 taatggcgat gctttccaac tgtaccagca actgatggcc catcgtcaca ccttcagcga
     1921 agcccacgcc gcccagagaa ctggcggcaa aaatcccggt catgatacag ccgacaatgc
     1981 cacaaacgcc gtgcacaccg aagacatcgc agggatcatc cacccgcagc aagcgtttga
     2041 gcatggtaac gccccacaaa cccgccagac cagctaccac gccgataatc aacgcgccgc
     2101 caaccccaat gtagccgcac gctggcgtca cgccgaccag accggcaatc gcgccagaac
     2161 acgctcccag cagagaaggc ttaccacgca gcgcccattc accgaagatc cagccaagaa
     2221 ttgccgccgc cgttgcgacc acagtattca caaatgccag tgccgcgatt tcattcgccg
     2281 tgcccactga cccggcgtta aagccaaacc aaccgatata gagaatggca gtcccggtga
     2341 agaccatcgg caggttgtgc ggtttaaacg cctctttacc gaagcctacg cgtttgccta
     2401 tcagatacgc gcccaccaga ccggcgattg ctgcgttaat gtgcaccacc gtgccacccg
     2461 cgaaatccag cgcaccgtga gaagccagca aaccaccgcc ccacaccata tgcgcaatcg
     2521 gaatgtaaga gagcgtcagc cataccacca cgaaaatcaa cacagctgag aagcggattc
     2581 gttccgccag cgccccaact atcaagccta cggtaatgca ggcaaacgat ccctgaaacg
     2641 ctacgtggat atactgataa atgctgccca tcaccgccgt cagttcgatg ttttttagca
     2701 tcaaccagtt aatgttgccg aagaagttgt tgccctcacc aaacgccagc gaataaccgt
     2761 aaaccaccca gagaatacag accagtgcaa acgtcaccgt cacctgcgtc agcatcgaca
     2821 gcacgttttt gccgcgaatc aacccaccgt aaaacagggc aatccccgga atagtcataa
     2881 acagcaccag cgcagtacaa atcatcataa acgcattgtc ggctttatcg gccaccgcag
     2941 gtgcagccat taccagtccc ggcagcatcg ccagtgaagc aagcccagtt tttatcgtcg
     3001 ctatcttcat ttttcgttcc tgttgctgtg tgccagagat tacagcgccg cttcgtcggc
     3061 ttcgccggta cgaatacgaa tgacgcgttg caattcagcg acgaagattt tgccgtcgcc
     3121 aatttttccg gtgtaagccg ccttactgac gatatcgatc acttcatcga gttggtcatc
     3181 agcaatcgcc acatcaattt ttacttttgg caggaagttg acgctgtatt ccgccccccg
     3241 gtacagctcg gcatgccctt tctgacgccc gaaacctttc acttcggtga cggtcaggcc
     3301 ctgaatacca atggaagata acgcttcacg aacgtcttcc agcttgaatg gtttgattat
     3361 cacggtcacc agcttcatag atcccctccg gtcagaattc ggtaatggtt ctgctacacg
     3421 aagaaggtat tgcaagcggt gtgccagaaa tgaaaaacga gaaagagagc aggaagttaa
     3481 gaagattgaa aatgaaaacg cactatgaca gtgcacagtg cgttacattt ttgcaccaac
     3541 gatgaaatat tgcgctattc aggcgctcaa tgattcctct tcacgcacgc tggctgccag
     3601 ctcttcgccc gcaagttgca gttgatacat ctgccagtag cgtccctggg ccgccagcag
     3661 ttgctggtga gtgccctgct ccacggcttg cccacgatga agcaccagaa tggtgtcggc
     3721 atcaataata gttgataagc ggtgagcaat caccaccagc gtggtatgtt cacgcaccgc
     3781 cgccagagca tgttgaatcg cctgttcagt accggagtca atgctggcgg ttgcctcatc
     3841 aaggatcagg atttgcggcg tctccaccag cacgcgcgcc agtgccagca gttgcttttg
     3901 cccgactgag agattatttc cctgctcgcc cagcggcgta taaatacaat cactcatgct
     3961 gcgcgccagc tccgccagtt gcacggtttc cagcgcctgc cagacgcgtt cttcggagat
     4021 atcccgcccc agcgtcacat tagcgaggaa ggtatccgcc agcaccaccg gatcttgctg
     4081 caccattgcc acgccctggc gcagcgcgct atgacttagc gaacttaatg gacgaccatc
     4141 aaggtgaatc tcaccttccg ttagcgggta atagcccatc aataaactgg cgagggtact
     4201 tttaccactg ccggtatgcc cgaccagcgc cacaaagtta cgcgaaggca cagagagatt
     4261 aatgttcttt agcaccagat tgtcatcgcg ataagcaaat gacacgttat cgacttcgat
     4321 ggtgccactc tgtaacgggt aatcatcatt gccatattgc tggcgcggtc cgtccatcag
     4381 ttcaaacacg cgctcaccag caacaacagc ctgttgcagc atcgcctgtt gcgtggtcag
     4441 ttcgattaag ggttcgttaa gtcgcccaag atagctgata aacgcataca acacgcccac
     4501 ttcaatggtg ccgctggcgg agaagccaaa cagcatcaat aagccacaaa gaatgagcga
     4561 tgaaaacaga ctcagcagcg gacgcagcag aaaaccgtcg aggcgcaggg tttgcatcct
     4621 agccatatag tgtgaacgac tggcctcccc catacgttcg ccaaatcgcg cctgctgacg
     4681 aaactgctgg ataacgctca tgccattgat gatttcgtta aagccgtcgt tgatatccgc
     4741 cagataggcg cgcacacggc ggacaatcgg cgtgctgtaa cgctggtata tcaccattac
     4801 caccagcacc accgggaaaa tcattatcgc caccagcgcc attcgccagt cgaggctgaa
     4861 catcgccacc agcatcgcgc ccaccagtgc ggcactgcgc aggacagttg ccactacggt
     4921 aacgtagaga tcgcggatca cttcagtgtc attagtgacg cgggaaatca cctgcccgac
     4981 gggttgggta tcaaactcgc ttaatggctg gcgtaacgca gcatccatca cgtcggtacg
     5041 caactgttgc actgcgccta ctgccgcccg attaaacagc agcgactgcg cgtaatgtag
     5101 cccggcggca aacagttgta gcccaacata cgccgcagcc agccctgcaa ccactttcaa
     5161 cggcaggtta tttttcgcta ccatattgtc gataaaatag ctgataagca gcggcccact
     5221 gacttctgcc gccgccgcaa cccacatcat caggaccgca atccccagcg gtttacgcca
     5281 cggcgaacca tacgctaaca ggcgcttgag agtcggccac agttggctaa aactacgcat
     5341 cgacggcctc ctcgcgaatt tccggaacgt cgtcgagcgc cgcctccagt tgttgatagc
     5401 gatacatatc gcgataccaa ccgctttgtt gcgccagctc atcatgattg ccacgctggg
     5461 cgatatgtcc gtgctgcatc acaataattt cactggcttc cgtcagtgcc gaaaggcgat
     5521 gggcactgat gattaccgtt cttccctgtc cccactgacg caggttatgc aggatctggt
     5581 gctctgtgcg tccgtccacc gccgaaagcg catcatcaag gatgaggatt tccgcgttga
     5641 ctaataacgc tcgagcaatg gagatacgct gtttttgccc accggaaagc atcacgccgc
     5701 gctcgcccac ttctgtatcg taaccttgcg gtagacgcaa aatatcgtca tgtacgctgg
     5761 ctaaccgcgc gacatgctca atctcttgct gggtggcatt cgggcaaccc agcgcgatgt
     5821 tattcgccac ggtgtcagaa aaaaggaatg gcgtctggct gaccaccgcc aggcggctac
     5881 gccagctatc gagttgtaac ttcgtcagag gaatatcatg aaagcgaata tccccctcgc
     5941 tgacgtcgaa atgacgctga atgagcgaca acagggtact tttgccggaa ccagtcggcc
     6001 cgcaaatacc cagcatctga ccgggtttca gggcgaaatt gacgttttcc agcgcaggat
     6061 ggtcagtctg cggatacgtg aactggtgaa tatttacatc cagttcgcca cgcccttccg
     6121 gcacgggttc gctaccatcg ttcaccaccg gcgcttccgc cagcatggcg cgaatacggc
     6181 tgtatgcagc actaccacgt tccacaatgt taaacatcca tgccagcgcc agcattggcc
     6241 aaatcatcag acctaaatac atcataaaac tggtgagctg gcccagcgtt aaactgccct
     6301 gcaccaccat ccaactaccg ccgccaatcg ccagcaagtt cgccatacca atcgcgatat
     6361 agatggtcgg gtcgaagcga gcatcaatac gcgccacccg catgtttttt ttgccggtat
     6421 cttcggcatc cgcggcaaat aacgccgact ggcgatcttc cagaccaaag gctttgatca
     6481 tgcggatact ggtgaggctt tcctgggtgc ggtcattaag actggaaaac gccgcctgtg
     6541 ccagcttaaa gcgttcatgc aaggcatcgc cgttgcgctt aatcatgatc gccatcactg
     6601 gcatcggtaa cagggcaaat aaggtcaact gccagctaat ttgcgtagac atcataatca
     6661 acacagcgca gcccattacc agtgaatcca ccagcgtcag caccccttct ccggcggcaa
     6721 acacgacgcg atcgacgtca ttggtcgcac gagccatgag atcacccgtg cgatgacgca
     6781 ggtaaaactc cgggtgctgc cggcttagct gacggtaata atcttcacgc agttcaacag
     6841 ccagttggta agacgcgcca aacagcaata cgcgccagac ataacgcagg agataaacca
     6901 caacggcaat cagcaccatg gtggcgatcc acatcaggct cagcccggta gtaaagtgtt
     6961 gttctgtcac gccatcgaca acaataccaa ccacttttgg cggaaccagt tgcagcatcg
     7021 cgataatgac aagcaaggcg acagccccaa gatagcgacg ccattcccga cggaaatacc
     7081 agcttaattg agcaaataat cgcacgcgtt gtgtcctgac ctgattctgg atatttattc
     7141 gatgggtaaa gaagtggtgt atttaatctg ctccatcgcg aagctggaag tgacgtccga
     7201 cagccccggc acgctgttta ccagacgctt ataaaactcg tcgtagcgtt tcatgtcggc
     7261 aacctggacg cgcatcagat aatcgtattc accagccatg cgccagaccc ccagcacttc
     7321 tggcatttcg gtaaccaccg tgacaaagcg gcaataccat tcgctgctgt gatgttgcgt
     7381 ttttatcagc acaaaggcgg tcaggccgag gcctattttt tccggatcca gcagggcgac
     7441 tttgccgata aggataccgt cgtcctccag ccgtttcagg cgcttccagc agggggtggt
     7501 tgtcagatta acggcttcag ccagtgcctg caaagagagg gtgcaatcct gctgcagtaa
     7561 ggccagcagc ttacggtcaa ttttatctaa catagccctt ccacggagaa tttttttctc
     7621 ttttaattct attttaaagg tgaaatcgcg acaatttatt ctgtgcggtt taaacaccac
     7681 ggcacaaaat gacaaattca acgcaaaaaa aaaccgggca attgcccggt gctggaaggg
     7741 atctcgttat tcgggggaat aaggtagatg tggatagtcc agccagtgcg tcaaatagtg
     7801 agagacagcc tgatttcggc aatgtccaat caccggtaaa tgcgggagct ccgcgcgcag
     7861 ttgcggcatc gcattgccca taataaaccc gctaccgacg ctgcctaaca tttcgcgatc
     7921 gttcatcgca tcaccaaagg ccatgcaatc gcgcaacgat aaacctaaat gttgggtccg
     7981 caccgtcaat gcagcgcctt tattgcagcc caccggcagc acttcgaggc aatccgtggc
     8041 ggaaaaacac aaatgtgcac gctcgcctaa tgcttcgtat agctggatct gcaagcgtgt
     8101 aagatcgtcg tgatcgccac agaagcagat cttggtgacg ctgccgagtg gcattttttt
     8161 gacatcgatt atctgataac gaaaaccgct atagacaaat acctgcaaca acgcagggct
     8221 ctctttcccg gtaaaccaac cgtcgtcatt gaagatatgc atgctggctc gggtatccca
     8281 ttgctgatac agcaccagct ccgcgacatc cgcaggtaaa tcatcacgat gtaaaagttc
     8341 accttccaga gaatgcacgc gcgttccgtt gccggtaatc aaatatgcat ccagcgatag
     8401 cgccccgaga atatgctgca tctccagcgc atgacgcccc gtggcaaaag tgagggtaat
     8461 gtcgcgttca cgcagtcgcg ccaaagtaga gagggttttc tcacctaaat gatggtcagg
     8521 catcaataaa gtgccatcca tatcaaatgc taccagacga gccatttctt tctccactct
     8581 gtgacacggt taatggtgat tgagtatcac ctgatatata cggaagtaat agtgaatagt
     8641 taaataatat tgttccgggt ttatatgcga ttgctcaacc gtcttaacca gtatcaacgt
     8701 ctgtggcaac cttcagccgc aaaccgtcac cgtcagcgaa ctggccgaac gctgtttttg
     8761 cagcgaacgc catgttcgta cgctgttgcg tcaggcacag gaggcgggat ggctggagtg
     8821 gcaggcgcag tcaggacgcg gaaagcgcgg acaattacgc tttctggtca cgccggaatc
     8881 gctacgcaat gcgatgatgg aacaggcact ggaaaccgga aagcagcaag atgtgctgga
     8941 gctggcgcaa ctggccccag gtgagctgcg cactctgtta cagccattta tgggcggaca
     9001 atggcaaaac gatacaccca cgttgcgtat tccctactat cgcccgctcg aaccgctaca
     9061 accaggcttt ttgcccggcc gtgccgagca gcatctcgcc gggcagatat tttccggcct
     9121 gacccgcttc gataataata ctcagcgccc gattggcgat ttagcgcatc actgggaaac
     9181 ctctactgac aggttacgct gggactttta tcttcgttca accctacact ggcataacgg
     9241 cgatgcagta aaagcctcac acttacacca gcgattattg atgctgttac aactgccagc
     9301 actggatcaa ttatttatta gcgtgaagcg tattgaagtc acccatccgc agtgtctgac
     9361 cttcttttta catcgccccg attactggct tgcgcaccgg ctggcgagct attgcagcca
     9421 tctggcgcat ccgcaattcc cactgatcgg cactggtcct tttcgcttaa cacaattcac
     9481 agcggagctg gtgcgcctgg agagccatga ttattaccat ttgcgtcatc cgctgcttaa
     9541 agcggttgag tactggataa ctccgccgct tttcgaaaaa gatttgggaa ccagttgtcg
     9601 gcatcccgtg caaatcacca tcggcaaacc ggaggagctg caacgggtca gccaagtcag
     9661 tagtggcatc agtttaggtt tttgctattt aacgttgcgc aaaagtcccc gactctccct
     9721 ctggcaggcg cgaaaagtga tctccattat tcatcaatcc ggtttattac aaacgttaga
     9781 agtcggagaa aacctgatca ccgccagtca tgcattactg ccaggctgga ctattccgca
     9841 ctggcaagta ccggatgaag tcaaactacc gaaaaccttg acgctggttt atcacctacc
     9901 gatagaactt cataccatgg cagaacgcct acaggcgaca ctggcagcgg aaggctgtga
     9961 actcacaatt atttttcata acgcaaaaaa ctgggacgac acgaccctac tggcacacgc
    10021 agacctcatg atgggcgaca gattaattgg cgaagcaccg gaatatactc tggagcaatg
    10081 gctacgttgc gatccactat ggccacatgt tttcgacgct ccagcatatg cacatctgca
    10141 atcgacgctg gacgcggtgc aagtaatgcc tgatgaggaa aaccgattta atgccctgaa
    10201 agcggttttt agccagttaa tggcagacgc gacgctgacg ccgctgttca actatcacta
    10261 tcgcattagt gcccctcccg gcgtgaacgg tgtgcgactg acaccgcgcg gctggtttga
    10321 atttaccgaa gcctggcttc ccgcgccgtc gcaatgaata gctggtccgg gttaatcgca
    10381 ggcgttacca taacgctttt attgttcaag caggattatc tatgaaacgt gctgtcgttg
    10441 tgttcagtgg aggccaggat tccaccacct gtctggtgca ggcattacaa caatatgatg
    10501 aagtccattg cgtgacgttc gattacggtc agcgacatcg cgcagaaatc gacgtggcac
    10561 gcgaactggc gctgaaactg ggagcacgcg cgcataaggt tcttgatgta acgctgctca
    10621 acgagctggc ggtcagcagc ctgacacgtg acagcattcc ggtgcctgat tatgaacctg
    10681 aagccgatgg catcccgaat acgtttgtcc cagggcgtaa tattttgttc ctgacgctgg
    10741 cggcaatata tgcgtatcag gtaaaagcag aagccgtaat taccggcgtc tgtgaaacgg
    10801 atttctccgg ctacccggat tgccgcgatg agtttgtgaa agcactaaac catgccgtca
    10861 gtttgggcat ggcgaaagat attcgttttg aaacgccgct gatgtggatt gataaagcgg
    10921 aaacctgggc gctggcagat tattacggca aactggattt agtccgtaac gaaacgttga
    10981 cctgctataa cggcattaaa ggcgacggtt gcggtcattg tgcggcatgt aatttacgcg
    11041 ccaacggttt gaatcattat ctggccgata aaccgacggt gatggcagcg atgaagcaga
    11101 aaaccgggtt gaggtaatta ttccgggtgt cgccggatgc ggcttgagca tccggcacca
    11161 caaaacgttt acttaaccat ctgctccagc ttttcgcgca attccccttc cagagctaat
    11221 gctttctgcg ttttaagatc aatacaaaca aacgtaataa gcgcatccgc taccacctgc
    11281 ccttccggct ccagtgtaat gacctggttt aagatgccgc ttttaccgtt taattgctgc
    11341 aactgactgg taatagttaa caggtcgctt aataccgctg gacgacgata gttaatattg
    11401 atattgacca cgacgaaggc gatgttatgg gccgtcatcc actgaaaact gtcgctattt
    11461 tccaccccat cccagcgggc ttcttcgaga aattcaaggt agcgggcgtt gttgacgtgc
    11521 tggtaaacgt cgagatgata tccacgaact ttgatttgtg tttgcatagc gcaataacct
    11581 tacgttgttg ttataagtac agaggtcata actggtatga cctcttcagt ctggcaaaaa
    11641 attgccacta tgcaaattaa ttacagggtt aataccgcca gattacgttc caccagcgaa
    11701 ttgcccatcc ccggcacctg ctttagatcc tccaccgttt taaacggacc gtactcttcg
    11761 cgataactga caatcgcctg cgctttcttc aggccaacgc cattcatcgc gcgggctagc
    11821 tcttccgcgc tggcattatt aatgctgacc cgggtgcctt cttcgtcact ggctttcgcc
    11881 ggtactgctg ctttactttg tgctgcagga gcttccgctt tggtttctac cgccgtcggt
    11941 ttcgccacag aagctgccgc cagcgcgcta tgagacattc cggcacaggc cagggacagt
    12001 gtaatgagca gtgctttaat tccgtgtttc atactgtttt ctccttgttt gttaacagtg
    12061 gaatcacctt aacgggagtg aaaacagtaa acaaatggca atgttcagaa atggaaaagg
    12121 ccgcgaaagc gaccttttct tgttacagct gcaacgcatt tttttgcgag gcggcttcag
    12181 gattattgct gttccagcgc atcgccaatt ttgattttcg cctctttacg caggttactc
    12241 atcagagctt caaagacgat ttgtgcgttg ttctgggtga taccctgcac catcgctttt
    12301 ttctgatctt ccggcattga accttgtttc acttcatcca gcgccagcag aaccacatta
    12361 ccttgcatat cggtcgccat accgtagctc ggtttgtctt tcgctggcag tggcagtgca
    12421 aacgccgcct ggctaatcgg gtcacgaccg gaacggctta aggttttcgg ctcgccaaat
    12481 ttcagaccgg cagcctgcat agcttccgca cctttgccgg ctttcaaatc aaccagcagt
    12541 ttctcagcat ccactttcgc ctgttgttca gctttgttgt gctgaaccag tgccttaact
    12601 tgttcctgaa catctgccaa cggtttcacc gcttccggtt tgtgctcgct gacgcgcagc
    12661 acgaatgcgc ggtcgccgtc tacggtgatg atgtcagagt tgatgcccga cgcgccgttt
    12721 tcacctacca gaccgccgtt aaagatagcg tcggcaaccg gcttgaagtt caactcttcc
    12781 ggcaggttat ctttgctgaa ccaacccgtc tgagtggctt taacgccggc agcttgctct
    12841 gcaccggcca gagactcggt gtcgttgctt gccgcatcgc tcactttctg ctgcagcgca
    12901 tagtacgcat cgagggcttt ttcgtgtttc actttcgccg caatgtcgtc acgtacttcg
    12961 tctaacgatt tcactttcgc tggctgaatg tcgtccagac gtacaatcag gaaaccgacc
    13021 gaagatttga tgacaccaga cagttggcct ttttctttca gaccagcatt tttcagttcg
    13081 tcggggatag tggcatcttc taaccaaccc atatcgccgc cgttacgggc agagataata
    13141 tcggcagatt tttctttggc taatgcagca aaatcaccgc ctttattcag ctcatcaagt
    13201 accgctttcg cttcatcttc agttttggtc tggatgatgc tgtagcgggt acgctgcggc
    13261 tgggtgaatt gatcctgatg ctgatcgtag tagctctgga tatccgcatc gctaaccggt
    13321 tgctgcatcg ttgcggcaac cagcttgatg taactcacgc ggaattgttc cggcgtcatg
    13381 aaattgtttt tgttttgttc gtagtagctg gcaatctcct gttcggtcac aggctgcttc
    13441 gccgccagcg cgttaacatc gatagtcgcc tcacgcacca cgcgttgttg cgcgaccagt
    13501 gccgccagct cgtcggtttc acctttcagc ataaaatcgg taccggcaac gccgttaatc
    13561 agctgttggg tagtgagctg gttacgcagc gcctgggcgt actgatcggc ggtcatcccc
    13621 atctggttga ggataccgtt atagcggctg ttatcaaatt tgccgtcaac ctggaaggct
    13681 ggggtcgcga aaatcgcctg tttaacctgc tcatcactga tacccagttt cagctcacga
    13741 gcgtactgat ccaacagcgc ctcgtcgatc agacgattca gcacctgttg acgcagggtt
    13801 ttcatatagc cttcgttcgc tgccagctcg gagtattgat cgcccagctg ttgctgcata
    13861 cgattacgct cgctgttaaa ggcgttctcg aactgcccac ggctgatttc ctggtcattc
    13921 acttttgcgg cgtagttatt gcctccgcca atcaggtaac cactcacgcc ggtcaatatg
    13981 aacgacacga taatgatacc gaaaataatc ttgagcacga gactgtttgc agccgtgcgt
    14041 aagctgtcca tcatggtgta acaacactcc gctgtagatg actgtgtacc tcacgcaacg
    14101 tatcgatgcg cgcaaaggcc tattgtgaca agaaacgggg gcaattgtca gcccacaaga
    14161 cacagaaagt caccgaatac aaataaaaaa ggcacatcag tagatgcgcc cttgaacttc
    14221 gtcacatccc cactggggac aacgcttagt ttaccgcgtc tttcagtgct ttacctgcac
    14281 ggaagctcgg tactttagcg gcagcgatgg tgatctcttt accggtctgc gggttgcggc
    14341 cagtacgggc agcacgctct ttaacggcaa aagtaccaaa acctaccagt gctacatcat
    14401 ccccttcttt cagagattca gttacggaag caataatagc atctaacgca cggccagccg
    14461 cagctttaga gatatcagcc cctgcagcaa tcttgtcgat caattgagat ttattcactc
    14521 ttctcttcct ctttataatt tatatcgcac ttgaatcctt caaggtacga acgcgcagca
    14581 gttatatcag gccagccatc cccttacaac accccttaat aagggcaagc ccgatccgct
    14641 atctaactta gcgagacaaa aaaaggctgg caagcccgaa ttagcctgcc agccctgttt
    14701 ttattagtgc attttgcgcg aggtcactat tttgcagtca caacctgcat gccagacggt
    14761 tcattttgca gcgccagagt cagaacttcc tcaatgcgct tcacaggatg aatgtccaga
    14821 tcggcaatta cgttgtcagg aatctcttcc agatcgcgtt tattttcgaa cggaattagc
    14881 actgttttaa tcccgccgcg atgcgctgcc aggagttttt ctttcaaacc accgatcggc
    14941 agtacctgac cacgcagagt gatctcaccg gtcattgcca catcggcacg aaccgggtta
    15001 ccggtcaggc aagaaaccag cgcggtgcac atagcaatac cggcactcgg accatctttc
    15061 ggcgtcgcac cttccggtac gtggacgtgg atgtcacgtt tttcgtaaaa atcagggttg
    15121 atccccagtt tttccgcacg cgcacgaacc accgttaacg ccgcctgaat agactcctgc
    15181 atcacttcgc cgagcgaacc ggtataggtc agtttgcctt tacccggaac acatgcggtt
    15241 tcaatggtca gcaagtcacc gcccacttcc gtccacgcca gaccgattac ctgaccgaca
    15301 cggttttcgt tatcagcgcg accatagtcg aaacgctgaa caccgaggta gtcatgcagg
    15361 ttatcgccgt taatttcgat atgttttaat gacttatcga gcagtaactg cttaaccgct
    15421 ttgcggcaca gtttggagat ttcacgctcc agaccacgca cgcccgcctc acgggtgtag
    15481 taacgaataa tgccgataat ggcgctatcg tcgacggtca gctcaccttt tttcagtgca
    15541 ttacgttcaa tctgcttcgg cagcaggtga cgtttggcga tgttcagttt ttcatcttcg
    15601 gtataaccgg agaggcgaat cacttccata cgatccagca gcggtgccgg aatgttcatg
    15661 gagttcgacg tcgcgacaaa catcacgtcg ctgagatcgt aatccacttc caggtagtgg
    15721 tcgctgaacg ctacgttctg ctctggatcc agcacttcaa gcagtgcaga ggccggatcg
    15781 cctcgcatgt cagaagacat tttgtcgatc tcatcgagca ggaacagcgg atttttcacg
    15841 cctactttcg ccattttctg gatcagttta cccggcatag aaccgatgta agtacggcgg
    15901 tgaccacgga tttccgcttc atcacgcacg ccgcccagcg ccatacggac atatttacgc
    15961 ccggtggctt tggcaatgga ctgaccaaga gaggttttac ctacccccgg cggccctacc
    16021 aggcagagga tcggtccctt gattttgttg acacggcttt gaaccgcaag gtactcaagg
    16081 atgcgatctt tcacgcgctc cagaccataa tggtcggtat caaggatttc ctgcgcctga
    16141 cgcaggtctt ttttgacctt gctgcgcgca ttccacggta cctgtaccat ccagtcgata
    16201 taaccacgca ctacggtcgc ttctgccgac atcggagaca tcattttcag cttctgcaac
    16261 tctgcttccg ctttctcttt tgcctctttc ggcatcttcg ccgcgtcgat tttgcgcttc
    16321 agggcttcgt tttcgtccgg cacgtcgtcc atttcaccga gttctttctg aatagctttc
    16381 atttgctcgt tcagatagta ctcacgctgg gatttctcca tctgcttttt aacgcggttg
    16441 cgaatgcgtt tctcaacctg cagcagatcg atttccgatt ccatcattgc catcagatat
    16501 tccagacgtt cgttaacgtc ggacatctcc agaacggact gtttgtcagc cagtttcagc
    16561 ggcatatgtg cagcaatggt atccgccaga cgcgccggat cgtcgatgct attcagcgac
    16621 gtcagcactt ctggtgggat ttttttgttc agcttgatgt agccttcgaa ctggctgatt
    16681 gcagtacgca ccagcacttc ctgttcccgc tcatcaatgg tcggcgactc cagatactcc
    16741 gccttcgcag aaaagtgttc gccattgtca gagagcgcag aaatacgcgc gcgctgtaac
    16801 ccctcgacca gcactttgac ggtgccgtca ggcagtttca gcatctgcaa tatagaggcc
    16861 acggtcccga cggtgaaaag atcgtttaca cccggctcat ccgttgaagc ttctttctgc
    16921 gcgaccagca taattttttt atcatggtcc atcgccgctt ccagacaacg gatagatttt
    16981 tcccgcccga caaataaggg gatgaccatg tgcggataaa ccaccacatc gcgcagcggc
    17041 aatacgggga tttcaatgcg ttcagaacgc tcaggattca tagagctctc tcttagttta
    17101 atttccgcca ggtaatcaga tgacacgact gtgcttcacg ccatctatta acatgtacgt
    17161 cagtatatgg ggatgtttcc cccacattca acgccgagaa tagaggaaaa attaaagggg
    17221 agataaaatc cccccttttt ggttaactaa ttgtatggga atggttaatt attcaccaga
    17281 tgcctgttgc gcttccggct taccataaat cagcaacggt ttgctttgac catcaattac
    17341 cgactcgtcg ataaccactt tttcgacgtc ttccatggac ggcagatcgt acatggtatc
    17401 gagcagtgcg gcttctacga tggaacgcag gccacgggca ccggttttac gcgccatcgc
    17461 tttcttagcg atagcatcca gcgcctcgtc acggaattcc agatccacgc cttccagatt
    17521 aaacagcgcc tgatactgct tggtcagggc gtttttcggc tctttgagga tctgaatcag
    17581 agcttcttcg ctcagttcat tcaacgttgc tacaaccggc agacgaccaa tgaactcagg
    17641 gataagacca aacttgatca gatcttccgg ttcaacctgc gccagcagct cgccttcgct
    17701 tgctttgtcg gacttcgctt ttaccgtcgc gccaaaacca atgccggagc cggtttctac
    17761 acggtgggaa atcactttat ccagaccggc aaacgcaccg ccacaaataa acaggatctt
    17821 agaggtatca acctgcaaga attcctgctg cggatgttta cgcccacctt gcggtggaac
    17881 agcagctacc gtaccttcga tcagtttcaa cagtgcctgc tgtactcctt caccggaaac
    17941 gtctcgggta atggacgggt tgtctgactt acgagaaatc ttgtcgattt catcgatgta
    18001 gacaatcccg cgctgcgctt tctgtacgtc gtaatcgcac ttttgcaaca gcttctgaat
    18061 gatgttttca acgtcttcgc ccacataacc ggcttcggtc agcgtggttg cgtcggccat
    18121 ggtgaacggg acgtccagca ggcgcgccag cgtttcagcc agcagcgttt taccggaacc
    18181 ggtcggaccg atcagcagaa tgttactttt gcccaactcg acaccattgc tggtatcgcc
    18241 gttgcgcaga cgtttgtaat ggttgtatac cgcgaccgcc agcacttttt tcgcctgttc
    18301 ctggccgata acgtaatcgt ccaggtggtt gcgaatttca tgcggcgtcg gtagcgcact
    18361 gcgttcacga tgcggtgcaa cttctttaat ctcttcgcga atgatgtcgt tacataaatc
    18421 aacacattcg tcgcagatat acacggatgg accggcaatc agcttgcgca cttcatgctg
    18481 gcttttgccg caaaaagagc aatacagcaa tttgcctgag ccatctttgc gtttatctgt
    18541 catgggtcaa aacctcttct ttgttctttg tgccgcacac gacgacgcaa atgccattct
    18601 caggcgcaag ccgcttacag cgttgtgccg ccctagataa gtatagcggc acagttgcgc
    18661 ctctggcatc aattacgatg ggtcagaatc gaatcgacca gaccgtattc caccgcttca
    18721 ggggcggaaa ggaagcgatc gcgctcggta tcacgttcaa tctgttctaa tgattgaccc
    18781 gtatgaagcg ccataagttc attcatgcgc cctttaactt tcagaatttc acgggcatga
    18841 atttcgatat cggtcgcctg gccctggtag ccgcccaacg gttggtgaat catcacgcgc
    18901 gaattcggca ggcaaaaacg tttacctttt gcccctgcgg tcaacaagaa agcgcccatc
    18961 gaggccgcct ggcccataca gatggtgctg acatcaggct tgataaactg catggtgtca
    19021 tagatagaca tcccggcagt gatcaccccg cctggggagt taatgtacag atagatatct
    19081 ttttctgggt tttccgcttc caggaacagc atctgcgcca caatcaggtt agccatgtgg
    19141 tcttcaacct ggccagtcag aaaaatgacg cgttccttaa gtagacgaga atagatatca
    19201 aaagagcgct caccgcgtga ggtctgttca atgaccatcg gcaccagcgc catatggggt
    19261 gcaaagttat ctcgttcgcc gctgtatgac atttccgtct cctggataaa attgaaaaaa
    19321 acctgctgta ccgattgtaa ccttatggac ggattatcag ctagtccctg tgttatgggt
    19381 acggcatcac catatttcaa gcataacaat cttttgttgt tacgctaaca ccgaaagagg
    19441 cttttcgcac ggctccatgc aaaattcatg acaaaaaaaa gcccaccacc tggcggtgac
    19501 gggcctttgt gcgaatttag cgcgttatgc tgcgtaaatt acgcctgctg gttcatcagc
    19561 tcgttgaaag tggtttcttt ttcagtcact ttcgctttcg ccagtacagc ttcaacagcc
    19621 tgttcttcca gagcaacatt gcgcatgttg tccatcagtt ctttgttttt gctgtagaac
    19681 tcgataactt ctttcggatc ttcgtacgca gaagccatct cttcgatcag gcctttcacg
    19741 cgctcttcgt cagctttcag ctcgttggtg cggataactt cgcccagcag caggccaaca
    19801 actacgcggc gtttagcctg ttcttcgaac agttcgcgcg gcagttccag agcttgtttt
    19861 tcgttgccac cgaaacgctg tgcagcctgg cgacgcagaa cgtcgatttc gctgtcgatc
    19921 agcgcagccg gtacgtcgat gtcgttagct tttaccagac cttcgatcgc ctgagactta
    19981 acgcggttac ggatggcgct cttcagctcg cgctccatgt ttttacgcac ttcagcgcgc
    20041 agaccttcta cggaaccatc ttcaacgccg aaacgtttga tgaattctgc agtcagttcc
    20101 ggcagttcac gctcttcaac tttcttcagg ttgatagcga atttcgctgc tttacctttc
    20161 aggttttctg cgtggtattc ttccgggaag gtcacgtcga tggtgaactc ttcgccagct
    20221 ttgtggcctt tgataccgtc ttcaaagccc gggatcatac gaccctggcc catcgccagt
    20281 acgaaatcag acgctttacc gccttcgaac tcttcgccgt ctacagaacc ggtgaagtcg
    20341 atggttacgc ggtcttctgc ttcaacagcg ccgtcttttt ctttccaggt cgcctgctgt
    20401 ttacgcagag tatccagcat gccgtcaacg tcagcgtcgg tcacttcaac gatcggtttt
    20461 tcaacttcga tcgcttccag accctgcagt tcaacttccg gataaacttc aaactctaca
    20521 gagtaagtga agtcttcacc cagcttgtat tcgcccggaa cataagtcgg tgcgccagcc
    20581 ggattgattt tttctttaat gatggcgtca atgaagttac ggctcatcag gtcacccaga
    20641 acgtcctggc gtacagacgc gccataacgc tgagcaacga tattcattgg cactttgcct
    20701 ttgcggaagc cgtcaatacg tacttttttc gcaacgttga ccagctcgct tttaacagcg
    20761 gtctcgatgc tgtcagcagc gatagtaatc gttacacggc ggccaaggcc ttgagtggtt
    20821 tcaactgaaa cttgcatctt gttacctcaa aaaatcacag tgctcggtca actcttaccc
    20881 cgcaagcaca ggttgttggc ttcgcggaac cgggatgttc ttataatcaa tcacattccc
    20941 tgttaccaga atcatcccga aagcattcaa ataggacgcg gcattatagc ggcatcactt
    21001 ttatgagtcg agaacggtta ttgcgcgctg tcgcgtattt tttcacaatt cccgggcaat
    21061 tttcgtctga atgctgggca aaaagcgcaa aatccagaca aaacaaaacg gcccgcaggc
    21121 cgtttttcat aaaatcttta gcaacatact ggaaaagcgc cggcagttgc aaatgcgttt
    21181 ttacgcgatg cttcctgctc cacgacaggg aggagaggca aagacggtgt cctgcaaccc
    21241 ttcccactcc ttaatagtgt aagtatgcag agccagcgca tgaacggtag tagagagttc
    21301 ctccgctaaa gtactgtaaa tcattcgatg acgattcaga aaacgttcac ccgtaaaacg
    21361 atcgctgacc agcacaactt taaaatggct ttcagagccg gctgggacat tgtgacgata
    21421 gctttcatcc actacttcga ggaatacggg ttggaacgcc gcccttaatt tttcttctat
    21481 ccgctcacgt atcatcatga aatatctcct ccgacaacgc tgagatgtca tccatccctt
    21541 taaatactag ccgcttttac cgtttccatt gcagcttaac aacaaatatt taggtttcgt
    21601 cctggttttt ccgtcaagcg tatgaagttt actgatttaa agattgaaac acaggcctcg
    21661 catcagcgtt tcttttacca ggggtgatta tttcgtcaac atgatgaatt tacatgcctt
    21721 acccacttcc cctcgccgtt ggcaatgtta tgatggcggg aatttttacc aaacacgctt
    21781 aagagtctct gagagcccga acatgttcaa aaaaatcctc ttcccgttag ttgctctgtt
    21841 tatgcttgca ggatgcgcaa aaccgccaac aactattgaa gtttccccga cgattacact
    21901 gccacagcag gatccaagcc tgatgggcgt caccgtaagc attaatggtg ccgatcagcg
    21961 taccgatcag gcgctggcaa aagtcacccg cgataatcaa atcgttaccc tgaccgcctc
    22021 ccgcgctctg cgtttcctgc tgcaagaagt gctggaaaaa cagatgaccg cgcgtggtta
    22081 catggttggt ccgaatggcc cggttaatct gcaaatcatc gttagccaac tgtatgctga
    22141 cgtgtcccag ggcaacgtgc gctacaacat cgctaccaaa gcggatatcg ccatcatcgc
    22201 taccgcgcag aatggcaaca aaatgaccaa aaactatcgt gccagctaca acgttgaagg
    22261 tgcgttccag gcctccaaca aaaatatcgc cgatgcggtt aacagtgtgc tgaccgatac
    22321 catcgctgat atgtctcagg acaccagcat tcacgaattc atcaagcaga acgcgcgtta
    22381 atttctgccc tctggcccgg tgcaagccgg gcctgtagac gcccatgtcc agtcaatatt
    22441 tacgtatttt tcaacagccg cgttcagcca tattgctgat cctgggtttt gcttccgggc
    22501 taccgctcgc cctgacatcc ggcaccttac aggcctggat gacggtcgaa aatatcgatc
    22561 tcaaaaccat tggtttcttc tctctggtag gccaggctta cgtttttaaa ttcctctggt
    22621 caccgctgat ggaccgctac acgcccccat tttttgggcg gcggcgcggt tggctgctcg
    22681 ccacgcaaat cctgttatta gtcgccattg cggcgatggg ttttctcgaa ccaggcaccc
    22741 aactccgctg gatggcggcg ctggcggtgg tgatcgcttt ttgctctgcc tcccaggata
    22801 ttgtcttcga tgcgtggaaa accgatgtgc tcccggcaga agaacgtggt gcgggcgcgg
    22861 caatcagcgt gctgggttac cgtttaggga tgctggtttc cggcggccta gccctgtggc
    22921 tggcagataa atggttgggc tggcagggca tgtactggtt gatggcggcg ctgttgatcc
    22981 cctgtattat cgcgacgttg cttgcaccag aaccaaccga caccattcct gtgcccaaaa
    23041 cgctggaaca agcggttgtt gcacctctgc gagatttctt tggtcgcaat aatgcctggc
    23101 ttattttgct tcttatcgtg ctgtataagc tgggcgacgc attcgccatg agcctgacaa
    23161 ccacgttttt gattcgcggc gtcgggtttg atgcgggtga agtaggcgtg gtaaacaaaa
    23221 cgcttggctt actggcgacc attgttggcg cattgtacgg tgggattttg atgcagcgcc
    23281 tgtcgctgtt ccgggcgctg ctgattttcg gcattttaca aggtgcgtct aacgctggtt
    23341 actggctgct gtcgattact gataagcatc tctacagcat gggcgcagcc gtctttttcg
    23401 aaaacctctg tggcgggatg ggcacatcag cctttgtcgc gctgttaatg acgctgtgta
    23461 ataagtcatt ttccgctact caatttgccc tgctctcagc gctttccgct gtagggcgag
    23521 tttatgtcgg ccccgtggcg ggttggtttg ttgaagcaca cggctggtcg acattctatc
    23581 tattctccgt cgccgctgcc gtaccggggc ttattttgct gctggtttgc cgccagacgc
    23641 ttgaatatac acgagtaaat gacaacttta tctcccgtac cgaatatccg gcaggttatg
    23701 cctttgccat gtggacactg gcggcgggcg tcagcctgtt ggccgtgtgg ttacttctgt
    23761 tgacgatgga cgcgctggat ttgacgcact tctctttcct gcctgctctg ctggaagtcg
    23821 gggttttagt cgccctttct ggcgtcgtgc ttggtggttt gctggattat ctggcgctac
    23881 gaaaaacgca tctgacgtaa tctgtaaata ttatttagct gtagtaatca ctcgccgtaa
    23941 ttattacggc taaataaaca tcagcatcgc ttattaatat ttgttctttt gtgcggctta
    24001 gcgtttggaa attattgacg ccatttataa attctatttt tcttacacga ttcagctaat
    24061 gagtctttat tttctcacca cccagttgtc actttaatga taattatttg ttaaataatt
    24121 gttttatttc gcattggtta taccaattgc ccaccaagac tccttctgca ctcccctttt
    24181 gttata
//
LOCUS       DAJTSA010000057        24089 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000057 DAJTSA010000000
VERSION     DAJTSA010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.057"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..178
                     /gene="yjbD"
                     /locus_tag="OO369_002444"
     CDS             <1..178
                     /gene="yjbD"
                     /locus_tag="OO369_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR5982692.1"
                     /translation="VLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEA
                     SFSWSANTSTRGRR"
     gene            complement(311..1183)
                     /gene="rluF"
                     /locus_tag="OO369_002445"
     CDS             complement(311..1183)
                     /gene="rluF"
                     /locus_tag="OO369_002445"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR5982693.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1531..2478
                     /locus_tag="OO369_002446"
     CDS             1531..2478
                     /locus_tag="OO369_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR5982694.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2548..3354
                     /locus_tag="OO369_002447"
     CDS             2548..3354
                     /locus_tag="OO369_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5982695.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3364..3771
                     /locus_tag="OO369_002448"
     CDS             3364..3771
                     /locus_tag="OO369_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR5982696.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3771..4265
                     /locus_tag="OO369_002449"
     CDS             3771..4265
                     /locus_tag="OO369_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR5982697.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4331..5128
                     /locus_tag="OO369_002450"
     CDS             4331..5128
                     /locus_tag="OO369_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR5982698.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            5139..5927
                     /locus_tag="OO369_002451"
     CDS             5139..5927
                     /locus_tag="OO369_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR5982699.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5979..7208
                     /gene="sorE"
                     /locus_tag="OO369_002452"
     CDS             5979..7208
                     /gene="sorE"
                     /locus_tag="OO369_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR5982700.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7590..8279
                     /gene="pepE"
                     /locus_tag="OO369_002453"
     CDS             7590..8279
                     /gene="pepE"
                     /locus_tag="OO369_002453"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR5982701.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8370..10001)
                     /locus_tag="OO369_002454"
     CDS             complement(8370..10001)
                     /locus_tag="OO369_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR5982702.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10221..13904)
                     /gene="metH"
                     /locus_tag="OO369_002455"
     CDS             complement(10221..13904)
                     /gene="metH"
                     /locus_tag="OO369_002455"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR5982703.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14104..14928
                     /gene="iclR"
                     /locus_tag="OO369_002456"
     CDS             14104..14928
                     /gene="iclR"
                     /locus_tag="OO369_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR5982704.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            15245..17429
                     /gene="espL4"
                     /locus_tag="OO369_002457"
                     /pseudo
     CDS             15245..17429
                     /gene="espL4"
                     /locus_tag="OO369_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(17398..19135)
                     /gene="aceK"
                     /locus_tag="OO369_002458"
                     /pseudo
     CDS             complement(17398..19135)
                     /gene="aceK"
                     /locus_tag="OO369_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(19319..20623)
                     /gene="aceA"
                     /locus_tag="OO369_002459"
     CDS             complement(19319..20623)
                     /gene="aceA"
                     /locus_tag="OO369_002459"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCR5982705.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20653..22254)
                     /gene="aceB"
                     /locus_tag="OO369_002460"
                     /pseudo
     CDS             complement(20653..22254)
                     /gene="aceB"
                     /locus_tag="OO369_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(22523..23452)
                     /gene="metA"
                     /locus_tag="OO369_002461"
     CDS             complement(22523..23452)
                     /gene="metA"
                     /locus_tag="OO369_002461"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR5982706.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            23609..24052
                     /locus_tag="OO369_002462"
     CDS             23609..24052
                     /locus_tag="OO369_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR5982707.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5944 a         6147 c         6107 g         5891 t
ORIGIN      
        1 cgtactgacc aactccgaaa ccaacagcat taagaaagag tacatcgata aattaatggt
       61 cgagcgtgag gctgaagcga aaaaagcccg ccaattgaag aaaaagcagg cttataaacc
      121 ggatcctgaa gcgtcattta gctggtcagc aaatacgtcg acgcgcggca ggcgctaatg
      181 aacaatcaac gcctgagatt ttcactcgtc tgtgtggaca caatcttccc ctcaccctaa
      241 ccctctcccc gaaggggcga ggggactgtc cgagctcttt ttggacgttt tcatcagtct
      301 gaggcgttaa tcagcgtccc ttctttttac gccccggcga ggtaaaacgc ttaccgttgg
      361 acgccgggcg accgcctttt tccgccgttt tttccatctt aacgactgga cgtttgatgc
      421 ccgctgtttt cggtttggcc ttcgctttag gttttgcctc ggaagaggaa ttttcgatga
      481 gcttaaagag gtcgattaac tcatcgtcgg ttaaatcgcg ccactccccc agcggaatgc
      541 cgcttaagct gacgttcata atgcgcgtac gttccagctt tttcacttca tagccgaaat
      601 gctcgcacat gcgccggatc tgacggttca gcccctgcac tagggtaatg cggaagacaa
      661 acggcgcttc ttttttgact ttgcactttt tcgtcaccgt cccgaggatc ggcacccccg
      721 cccccatacc acgaataaat tcatcggtaa tcggtttatc gaccgtcacc agatactctt
      781 tctcatgatc gttacccgca cggaggatct tattcaccag atcgccgtga ttggtgagga
      841 aaatcagccc ctgggagtct ttatccaggc ggccaatcgg gaacacgcgt ttgctgtggt
      901 taacgaaatc gacaatgtta tcgcgctcgc catcttcggt ggtgctgaca ataccgacgg
      961 gcttgttcag ggcgataagt accaaatctt cggcttcccg aggctcaatc aactgaccgt
     1021 ttactttcac aatgtcgccg ggtttcacct gatcgccaat ggtggctcgc ttgccattaa
     1081 ggaacacatt gccttgctcg atatagcgat ccgcttcgcg gcgtgagcaa attccgcttt
     1141 cgctgatgta tttatttaaa cggactgatg agtcgggcag cataaattct cctgtaaaac
     1201 gcggaatata ccgtacctct tagcggggaa aaacagggtt atgcggcaac gtcgaaatta
     1261 aatgtgatct gccacgcctt ttatcacaaa agtgtctccg gaagtacgat gcaaatttgc
     1321 ctggcaaaat aaggataatc aacatattac gaatcgctgc acacctgtgc tgatgactac
     1381 tacgcagaga aacaaatgtg caagagaaag cgctatttct ccccatgcaa agagtgaaaa
     1441 atcgctttga aaggccctca tgatggggtt atatcagatc aagcggtgag aaagcgtatt
     1501 tgcacaaatg tgcagaacca ggaggcggca atggaaaaca gtgacgatat ccgtttgatt
     1561 gtgaagattg cccaactcta ttacgaacag gatatgacgc aggcgcaaat cgcgcgcgaa
     1621 ctgggtattt accgcaccac catcagccgc ttgcttaaac gaggccgcga tcagggaatg
     1681 gtcaccatcg ccatcaacta tgactacaac gaaaatctct ggctggagca gcaactgaag
     1741 caaaagtttg gcctgaaaga cgttgtggtg gtgtcgggaa atgatgagga tgaagagact
     1801 caactggcga tgatggggtt acacggcgcg caactgctgg atcgcttgct ggagcctggc
     1861 gatattgtcg gtttttcctg gggtcgcgcg gtgagcgcac tggttgaaaa cttgccgcag
     1921 gcggggcaat cgcggcagtt aatctgcgta ccaattattg gcggaccgtc cggtaaactc
     1981 gaaagccgct atcatgtaaa cacattaacc tacagcgcgg cagcgaagct gaaaggggaa
     2041 tcgcatctcg cggattttcc ggctctgctg gataacccat taattcgtaa tgggatcatg
     2101 cagtctcagc actttaaaac catctctgcc tactgggata atctggatat cgccctggtg
     2161 ggaattggct caccggccat tcgcgacggc gctaactggc atgcgtttta tggtggtgaa
     2221 gagagtgacg acctgaatgc ccgccaggtt gctggcgata tttgctcgcg cttttttgat
     2281 attcacggcg caatggttga aacgaatatg agcgaaaaaa cgctctctat cgaaatgaat
     2341 aaattaaagc aggcgcgata ttccattggc attgccatga gtgaagaaaa atacagcgga
     2401 attattggtg cactgcgtgg aaaatatatt aattgtctgg taacgaatag cagcacagct
     2461 gaactattac tgaaataaaa atcttcccga tgtttaccgt aataaaaatt acggcgggtc
     2521 cctattatct aaaatcagga gtctgttatg caaacgtggt taaatttgca ggataaaatc
     2581 attattgtca ccggcggcgc atccggtatt ggtctggcga ttgtagagga attattagca
     2641 caaggcgcga atgtacagat ggtcgatatt cacggtggcg atggtcaata tgaaggtcat
     2701 aaaggctatc agttttggcc gaccgatatt tctagcacaa aagaggtaaa tcatacggta
     2761 gcggaaatta tccagcgttt tggtcgcatc gacggtctgg tcaataacgc cggggtcaat
     2821 ttcccgcgtc tgctggtcga tgagaaagcg cctgccgggc agtatgaact caacgaagct
     2881 gcattcgaaa aaatggtcaa tatcaaccag aaaggtgttt ttctgatgtc gcaggcggtg
     2941 gcgcgacaga tggtcaaaca acatgatggc gtgattgtga atgtttcctc agaaagtggg
     3001 ctggaaggct cagaaggaca aagctgttac gccgctacca aagccgcgct caatagcttc
     3061 acgcgctcct ggtcgaaaga gctgggtaaa cacggtatcc gtgtggtcgg tatcgcgccc
     3121 gggattctgg aaaaaaccgg gctacgcacg ccggaatatg aagaggcgct ggcgtggacg
     3181 cgcaatatca ccgtcgagca gctgcgtgaa ggctatacca aaaacgccat tcctattggg
     3241 cgcgccggaa gattagcaga agtagctgat tttgtttgtt atctgttgtc tgaacgcgcc
     3301 agctacatca ccggagtaac cactaacatt gcgggcggca aaacgcgcgg ctaaggaggc
     3361 tttatggtca acgccatttt ttgtgcccac ggcaaattag cttgcgccat gctggaatcg
     3421 gttcagatgg tttacggcaa cgccaatgtt gaagccgttg catttgtgcc cggtgaaaac
     3481 gccggagaca ttgcggtaaa gctggaaaag ttagtaagca ttcacaatca agatgagtgg
     3541 ctgattgccg tggatttaca gtgcggcagc ccgtggaatg cggcagcaat gcttgcgatg
     3601 cgcaatcctc gtttgcgggt aatcagcggc ctttctctgc cgctggcgct cgaactggtt
     3661 gataaccagg acagcatgaa tgttgatgaa ctgtgtgaac acctgacgca aatcgcaaag
     3721 caaacctgcg ttgtctggaa acaactggca acgacagagg aggatttctg atgaacatta
     3781 cgctcgcccg cattgatgac cgtctgatcc acggccaggt cactaccgtc tggtcgaaag
     3841 tggctaacgc ccagcgcatt attatctgca atgacgaagt ttataacgat gaagtgcgcc
     3901 ggacattatt gcgtcaggct gcgccaccgg gaatgaaggt taacgtggtc aatattgaaa
     3961 aagccgtcgc ggtttatcac aacccgcaat atcaggacga aaccgttttt tatcttttca
     4021 ctcgcccaca ggatgcctta gcgatggttc gccagggggt aaaaataggc accctgaata
     4081 ttggcggcat ggcctggcgg cccggtaaaa aacagttaac caaagcggtt tcattagatg
     4141 atgatgatat taacgcattt catgagttaa ataatcttgg tgtcatactg gatttacgcg
     4201 tcgtagcgtc agatccctca atcaatatta tcgacaaaat aaacgaacag ttaatcgcaa
     4261 attaaataat agcgcctgtg tacatatgct ttaacaggca gggatgacga cacttaaaag
     4321 gtgacaaatc atggaaataa gtaccctaca aatcattgct atatttcttt tttcctgtat
     4381 tgccggaatg ggcagcgtgc tggatgaatt tcagactcat cgcccgttaa ttgcctgtac
     4441 ggtgattggt ttaattctcg gtgatttaaa aaccggaata atactcggcg gtacgctgga
     4501 attgatagct ctctgctgga tgaacgtcgg cgcggcgcaa tctccggatt ctgcactcgc
     4561 cagcataatc tccgccattc tggttatcgt tggtcagcaa agcatcgcca ccggaatcgc
     4621 catcgcgttg cctgtggctg cggcaggcca ggtgctgacg gtgtttgccc gtaccatcac
     4681 cgtagtgttc cagcacgcgg cggataaagc agcagaagag gcgcggtttc gcactctcga
     4741 tattctgcat gtctccgcgc ttggcgtgca ggcgctgcgc gtcgccattc cggcactgat
     4801 tgtctcactg ttcgtcagcg ccgatatggt cagcaatatg ttaaacgcca ttcccgaatt
     4861 tgtgacccga ggactacaga ttgctggcgg ttttatcgtg gtggtcggtt acgccatggt
     4921 gctacgtatg atgggcgtga aatatttgat gcctttcttt ttcctcggtt tcctcgcagg
     4981 tggctacctc gatctcagcc tgctggcgtt cggtggcgtc ggcgtgatca tggccctggt
     5041 ctacatccag ctaaatccac agtggtgtaa agctgaacct caactccagg ccaccgcctc
     5101 caccgccctt gaccaacttg acgattaacg gagccatcat ggaacagaga aaaattacac
     5161 gcagcgatct ggtgagcatg tttctgcgct ccaacctgca acaggcgtcc tttaactttg
     5221 aacgtattca cgggctgggc ttttgctacg acatgatccc cgccatcaag cgactttacc
     5281 cattaaaaga ggatcaggtt gcggcgctca ggctacacct ggtgttcttc aataccacgc
     5341 cagccgtatg tggcccggtc atcggcgcgg aaattgatga cggtaccatc aacggcatca
     5401 aagtcggtct gatgggacca ttggcaggag ttggcgatcc actggtctgg ggaacgctgc
     5461 gcccgattac cgccgcgctc ggcgcatctc tggcactttc gggcaacatt ctcggcccgc
     5521 tgctgttctt ctttattttc aacgcggtgc gtctggcgat gaagtggtat ggcctacagc
     5581 tcggctttcg caaaggggtg aatatcgtca gcgatatggg cgggaatgtg ctgcaaaaac
     5641 tcaccgaagg cgcgtcgatt ctcggactgt ttgtgatggg cgtgctggtg accaaatgga
     5701 cgtcaatcaa cgtaccgttg gtggtttcac aaacgcatgc cgccgatggc tccaccgtca
     5761 ccatgaccgt gcagaacatt ctcgaccaac tttgccctgg tttgctggcg ctcggtctga
     5821 cgctactaat ggttcgtctg ctcaacaaaa aaattaaccc ggtatggctg attttcgccc
     5881 tgtttggctt agggattatc ggcaatgctc tgggcttcct gtcctgattc ttcgccccgg
     5941 cacgactgcc gaggccatcg ctcaacatga ggtggtttat gaaaacaaca gctctgcgtc
     6001 tttatggtaa acgtgattta cgcctggaaa cctttgacct tcctgaaatg caggaggatg
     6061 aaatcctcgc aacggtggtc actgacagcc tgtgcctctc ttcctggaaa gaggccaatc
     6121 tgggtgaaaa ccataaaaaa gtacccgacg atgtgacgac caacccaatc atcatcggcc
     6181 acgaattttg cggcgatatt ctggccgtgg gtaaaaagtg gcagcacaaa ttccagccgg
     6241 gtcagcgtta tgtgattcag gccaacctgc aactccccga ccgcccggat tgccccggct
     6301 actccttccc gtgggtagac ggcgaagcga cgcatgtggt tattcccaac gaggtcatgg
     6361 aacaatattg cctgctggca tacgacggcg aaacctattt tgaaggctca ctggttgaac
     6421 cgctttcctg cgtgattggc gcgttcaacg ccaactatca tcttcaggaa ggtagttata
     6481 accacacgat ggggattcgc ccgcaagggc acacgctgat cctcggcggc accggaccaa
     6541 tgggactgtt ggcgattgat tatgcgctgc atggaccagt taacccgtcg ctgctcgtca
     6601 ttaccgatac cgacaacgac aaattgagtt atgcgcgcaa gcactatcca tcagaaccgc
     6661 aaacactgat tcattatctc aatgccaccg atgcagcatt tgatacgtta atggcgctga
     6721 gtggcggtca cggcttcgat gatattttcg tctttgtgcc taatgaagga ctggtgactc
     6781 tcgcctcttc cttgctggcg acagatggtt gcctgaattt cttcgccgga ccgcaggata
     6841 aacatttcag cgcgccaatt aatttctacg atgtgcatta tgcatttacc cactacgtgg
     6901 gcacgtcagg cggcaatacc gacgacatgc gcgcggcggt caaactgatc gaagagaaaa
     6961 aagtgcaggc cgcaaaagtg gtaacacata ttcttgggct gaatgccgcg ggcgaaacca
     7021 cgcttgaatt gcctgccgtc ggcggcggca aaaagctggt gtataccggg aaatacctgc
     7081 cgctgacgtc actcacgcag attcaggatc aagcactggc ggcgattctg gcgcgtcatc
     7141 aggggatctg gtccggtgag gtggagcaat acctgctcgc ccatgcagag gcaatttccc
     7201 atgattaatc gcgctactta actctgcatg tcgctggctg gtcgccccag caacttcgcg
     7261 accggatgtg cggctgattg ctgaagcagc acaatttgca cgagaaggat aaaaaatatc
     7321 agcgccttaa ggggcgctca aattggtggc aaagtacaca ttgtttatgc cggatgcgga
     7381 atgaagcctt attcggtcta cataattttg aaaatccaat atattgcagt tatctggtag
     7441 gccaggtaag cgtagcgcat caggcaatgc tgtgtttgtc atcagtctca gcgcccggtg
     7501 caatacgtga catcgtgagc catgtcagta agtggttaaa ttcgcagtgg tagcatgcct
     7561 tccgtgattt cttactaacc ggagaagtaa tggaactgct tttattgagt aactcgacgc
     7621 tgccgggtaa agcctggctg gaacatgcac tgccgctaat tgctgaacag ttgcagggtc
     7681 gccgctcagc ggtgtttatc cctttcgctg gcgtaacgca gacctgggat gattacacag
     7741 agaaaacggc tgcggttctc gctccgctgg gtgtttctgt caccggtatt catagcgttg
     7801 tcgatcccgt tgccgcgatt gaaaatgcgg aaattgtgat tgtcggcggc ggtaatactt
     7861 tccagttgct gaaacagtgc cgcgagcgcg ggctgctggc tccaattact gacgtggtta
     7921 aacgtggcgc gctgtatatt ggctggagcg caggcgctaa ccttgcttgc ccaaccattc
     7981 gtaccaccaa cgatatgccg attgtcgatc cgcaaggttt cgatgcgcta aatctgttcc
     8041 cgttgcaaat caacccgcac ttcaccaacg cgctgccgga aggccataaa ggtgaaaccc
     8101 gtgagcagcg tattcgcgaa ctgctggtcg tcgcgccaga actgacgatt attggtctgc
     8161 cggaaggtaa ctggatcaca gtgagtaaag gtcacgctac gctgggtggc ccgaacacca
     8221 cttatgtgtt taaggctggt gaagaagcgg ttccgctgga agctggtcac cgtttttaag
     8281 tgaattgccg gatgcatgat ccggcatctt gttcatagtg cctgatgcga cgcttgacgc
     8341 gtcttatcag gcctacagga ttcacaatat taatactcgt cccgtccttc atcttcatcc
     8401 ggctgttcca gcacactgta agccaccgaa caaaacagcg agttcaggcg ctgcatatct
     8461 cccagtaagc ctaaatgtag cgaactggtt tcaatgctct gcacgttttg ctgatgcagg
     8521 cgatcgacgt gggcgtggga atagcggcga ttaagaatgc gaaaacgatg tttgctgcga
     8581 cgcaaacgac gagcgctggt gacatcgcca gagaagaaca ccgacattgc cagctttaaa
     8641 ttactgagca attgctcata gagcgcatcc agttccttca acccatcaag cgaaaatgcc
     8701 cgccgtgctg ccagcgattt atcggcaatt tcgctgccca tgcgctcgac gatatcggag
     8761 gcctgttcaa ggttgagcga catttcgatg atctccgccc agcggcgcga ctcttcctcc
     8821 gccaactctt cttttggcat ccgcgccaga tacagcttaa tggcggtgta gagaacgttg
     8881 atatcatctg ccagcttacg cagctctttc tcctgccgtg gctcgccgtg catcacttta
     8941 ttcagccctt ccatcatctg ttccatggcg tcgccaatgc gcagggtttc gcgcgcggcg
     9001 ttcgccagag caagcgtggg cgtgtccagc gcgctgacat ccagatgttt aggccgtagc
     9061 tgggtatcca gttccggttc atcgcgaata atcgttttgc aaaaccgtgc catcgggtca
     9121 acaaatggca gcatgaccag gcaacgtaca aggttgtaga agacgtggaa atagatcacc
     9181 agttccgctt ttggcaatga cagcttcccc attgtctctg ccagcaaatg gacaaacggc
     9241 aggataatca ggctacccac cagcttaaac agcagactac ccagcgcgac acggcgggct
     9301 gcggcattgg cggcactgtt gttgagcatc gccagcaggc cggaaccgag gttagctcca
     9361 atcaccagac agagcgccac ggggaaggag ataatgcctg cagcggtcag agtcgcggtc
     9421 agcagtacag cagcaaggct ggagtagctg ataatggcga acatcgcgcc aatcagcgca
     9481 tccagcagaa tatcgccggt cagcgaggca aagatcacct gaacgccgtt tgcctgggtg
     9541 atcggcgtta cggcctgcac aatcaactcc agcgccagca aaatcagccc aagaccaata
     9601 ccgacgcggc ccagttgccc ggcgcgtgac tgtttgcgtc cgaggaaaaa aatcacgccg
     9661 ataaaaataa gtaacggtga cagccaggat aagtcgaagg tgagaatacg cgccattagc
     9721 gccgtcccga catctgcccc cagcacaatg accagagccg gtgcgagggc taccagatcc
     9781 tgagcgacga acgaggtcac cagcatggtg gtggcattac tgctctgtac cagtgcggta
     9841 acgccgatcc ccgcgcaaaa ggcgagcggc tttttttcga cgctccggct aaggacagta
     9901 cgcaaacgcg cgccgaagac gcgcattacg ccggttcgaa caatatgagt cccccagacc
     9961 agcagggcga cggcagaaag caggtgaagc agcgttaaca cgagttcaga ttctccttat
    10021 cgttatatgt tcctgaagca attgaatgac caaaggaccg gaaacacgct gactggcatc
    10081 tttactgtgc cttttgacat tcgtttttcc ggctaagatt attccagcct gtttccagta
    10141 taagtgtttc atattaataa agagacaggg cactcattga gcgccctgtt tgttgtaagg
    10201 aaaagtgaca gatttgtgaa tcagtccgcg tcatacccca gattcggtgc cagccagcgc
    10261 tcaacatcgg agacgctcat acctttacgg cgggcatggt cttcaacctg atcgcgctga
    10321 atttgcgcca ccgcgtagta cttgctgtcc ggatgactga aataccaacc cgaaaccgat
    10381 gcaccaggcc acatcgcgaa agattctgtg agtttcatgc cagtatgttt ttccacttcc
    10441 agcagctccc agatggtggc tttttccgta tgttccgggc aggccggata gcccggtgcc
    10501 ggacggatgc cctggtagtt ttcgcggatc agttcttcgt tgctgaggtt ctcgttcggc
    10561 gcatagcccc agtagacttt acgcacacgc tcatggagat actccgcaaa ggcttcggct
    10621 aaacggtcgg caagcgcttt caccatgatt ttgttgtaat catcgtgctg cgcttcaaag
    10681 gcatcagcca gtgcgtcctc ttccagcccg ccagtcacgg caaatgcgcc gatgtaatct
    10741 gctttaccag aaagcttcgg cgcaacgaag tcagcgagac agtagttagc gaatcccgtt
    10801 ttttcggtct gttgacgcag atggtggctg acgttgatca catgggtgcg cgtttcgtca
    10861 cggtagattt caatgtcatc gcccacacgg tttgccggga acaggcccac cacgccacgc
    10921 ggattcagcg ttttctcggc gcttaattta tccagcatgt cgttggcgtc tttaaacagc
    10981 cgctgcgcct caacgcccac cacttcatct tccagaatgc gcggatactt cccggccagc
    11041 gaccaggtca taaagaacgg tgtccagtcg atgtaattac gcagcgtttc gatgctggct
    11101 tccacttcct gcacgccgag acggtgcgcc accggcggcg tgtaagcctg ccagtcaaat
    11161 gcgaaatcgt tatcgcgcgc cgcttccagc gtgaccggtg gtgtgcgtgg tttcttgcgc
    11221 ccgtgctgaa tacgcacggt ttcgtactcc ttgcaggtac gagcgacaaa atcatcacgc
    11281 tgagtatcgg aaagcagcgc cgctaccaca ccaacggtgc gcgaggcatt ctgcacatac
    11341 accgtagggc cgctgtagtt ctgctcgatt ttcaccgccg tgtgcgcttt tgaggtggtc
    11401 gcgccgccaa tcagtaacgg aatggcgaag ccctgacgct ccatctcttt cgccacgtta
    11461 accatctcgt ccagcgacgg cgtgataagc cccgaaaggc caatcaaatc agcattcact
    11521 tctttggcga tacggagaat tttttccgca ggcaccataa cgccgagatc gacaatttcg
    11581 tagttgttac attgcagcac cacaccaacg atatttttac cgatgtcgtg gacatcgcct
    11641 ttcacggtgg cgatcaccat cttgccgttg gttttaccct gctctttgct ggcttcaata
    11701 aacggttcga gatacgccac tgcctgtttc atgacgcgcg ccgatttgac cacctgtggc
    11761 aggaacattt tgccttcgcc aaacagatcg ccgaccacgt tcatgccgtc catcaacggg
    11821 ccttcaatca cttcaatcgg gcgcgtagcc tgctggcggg cttcttcagt atcctgctcg
    11881 ataaactcgg ttatgccttt gaccagcgag tattccagac gtttattcac gtcccacgag
    11941 cgccactccg cttgctgggc gttagcagtg tcgtccgctt tactgccgcg atatttctcg
    12001 gcaagctcca gtaaacgctc agtgccatcg tcacgacgat taagaatcac atcttccacc
    12061 gcgtcgcgca gttcagcggg taggtcgtcg taaatcgcca gttgcccggc gttgacgatc
    12121 cccatatcca tgccattgcg aatagcgtag tagaggaaca ctgcgtgaat ggcttcgcgc
    12181 accggatcgt tgccacggaa tgagaaagaa acgttagata cgccgccgga aatcagcgcg
    12241 tgcggcagtt cgcgtttgat gtcttcacac gcgccgataa agtcctgcgc gtagttgttg
    12301 tgctcttcaa taccggtggc gaccgcgaag atgttcgggt cgaagatgat atcttctggc
    12361 gggaagccaa cctcttcggt gaggattttg tacgcccgac ggcaaatctc gattttccgt
    12421 gcgcgagtat cggcctgtcc ctgttcgtca aaggccatca ccaccactgc cgcaccgtag
    12481 cgacgcaaca atttcgcgtg atggataaag gcgtcgacgc cctctttcat cgagatagag
    12541 ttaacaatgc ctttgccctg gatacacttc agaccttttt caatgacatc ccattttgag
    12601 gagtcgatca taatcggcac gcgagcgata tccggttcac cggcaatcag attgagaaaa
    12661 cgcaccatcg ccgcttcggc atcgagcatc ccttcatcca tgttgatatc gataatctgc
    12721 gcgccgtttt ccacctgctg acgcgccaca tccagcgcct cgctgtattt ctcttctttg
    12781 atcaggcgct tgaacttagc agagccggtg acgttggtgc gttcacccac gttcacaaac
    12841 aggctatcgt cgccaatgtt cagcggctcc aggccagaca aacggcaggc tacgggaatt
    12901 tccggcagtt tgcgcggcgc taatccttct actgcacgac tcatcgctgc aatatgttgc
    12961 ggcgtggtcc cgcagcaacc accgacgata ttgagaaagc ccgcttgcgc ccattcacgt
    13021 atctgttttg ccatcgtgtc ggcgtcgaga tcgtattcac caaaggcgtt aggcagcccg
    13081 gcgttcgggt gggcggtgac gtagcattcc gcaatccgtg atagttcctg cacgtactgg
    13141 cgcagttcat cgggccccag cgcacagttc aggccaaagg tcagcgcttc ggcgtggcgc
    13201 aatgagttgt aaaatgcttc ggtggtctgc ccggagagcg tgcgcccgga ggcgtcggtg
    13261 atggtgccgg agatcataat cggcagctca acgcccagcg cttcaaactc cgttttcacc
    13321 gcaaagaccg ccgctttggc gttaagggtg tcgaaaacgg tttcaatcag gatcagatcc
    13381 gcaccacctt ccaccagcgc tttggtggat tcgcgatagg ccgccgccag ctggtcaaaa
    13441 gtgatgttgc ggaatgccgg atcgttgacg tccggagaaa tagacgccgt gcggttggtt
    13501 gggccaagaa caccggcaac gtagcgcggt ttctccggcg tgcgcgcggt ccactcgtca
    13561 gcacaggcgc gcgccagttt cgccgccgca aagttgattt ccgccgacag ggattccatc
    13621 tggtaatccg ccatcgcaat ggtcgtggag ttgaaggtgt tggtttcgat gatatccgcg
    13681 cccgcttcaa agtaggcgtt gtggatagcg gcgatcactt ccggtttact gagtaccagc
    13741 aggtcgttgt tgcctttgag gtcgcatggc cagtcggcaa agcgttcacc acgaaaatcg
    13801 gcttcgttca gtcgatagct ctggatcatg gtgcccatac cgccgtccaa caccagaata
    13861 cgttcattta actgcgcacg cagttgttcc actttgctgc tcacacttgc tcccgacacg
    13921 cttaacccag atttaataaa aattcaacaa accatactgg aataaacgca tctgtggtaa
    13981 aagcgaccac cacgcaacat gagatttgtt caacattaac tcatcggatc agttcagcaa
    14041 ctattgcatt agctaacaat aaaaatgaaa ataatttcca cgatacagaa aaaggaggct
    14101 gtcatggtcg cacccattcc cgcgaaacgc ggcagaaaac ccgccgttgc caccgcacca
    14161 gcgactggac aggttcagtc tttaacgcgt ggcctgaaat tactggagtg gattgccgaa
    14221 tccaatggca gcgtggcact cacagagctg gcgcaacaag ccgggttacc caattccacg
    14281 acccaccgcc tgctaaccac gatgcaacag cagggtttcg tgcgtcaggt cggcgaactg
    14341 ggacactggg caatcggcgc acatgccttt atggtcggca gcagctttct ccagagccgt
    14401 aatttgttag caattgttca ccctatcctg cgcaatctaa tggaagagtc tggcgaaacg
    14461 gtcaatatgg cggtgcttga tcaaagcgat cacgaagcga ttattatcga ccaggtacag
    14521 tgtacccacc tgatgcgaat gtccgcgcct atcggcggta aattaccgat gcacgcttcc
    14581 ggtgcgggta aagccttttt agcccaactg agcgaagaac aggtgacgaa gctgctgcac
    14641 cgcaaagggt tacatgccta tacccacgca actctggtgt ctcctgtgca tttaaaagaa
    14701 gatctcgccc aaacgcgcaa acggggttat tcatttgacg atgaggaaca tgcgctgggg
    14761 ctacgttgcc ttgcagcgtg tattttcgat gagcaccgcg aaccgtttgc cgcaatttct
    14821 atttccggac cgatttcacg tattaccgat gatcgcgtga ccgagtttgg cgcgatggtg
    14881 attaaagcgg cgaaggaagt gacgctggcg tacggtggaa tgcgctgact ttttctggcg
    14941 ggcagaggca atattctgcc catcatacct gagtggcaat agaataaggg tgtctgttaa
    15001 tcgcattgac gccaaaataa cttaatgtca tacacttcac acacttttat taactcagca
    15061 ttatttttaa acatcaaacc acttaattat taacaaaatc gcaagatctc attccaccga
    15121 aacttttacg gctgcattat gagattgagc gatttatgat atttttttag ttgtaatcat
    15181 attaaatttt ttcactgttc tgatagttaa aattcaagac atcaataaac aatgagatat
    15241 ttaaatgatt actcgtattc ctcgtagttc tttctctgca aatattaata atacagccca
    15301 gacaaatgaa caccaaaccc tgagtgaatt gttttataaa gaactcgagg ataaattttc
    15361 tggcaaggag ctggcgacgc ctctattaaa aagcttctca gagaactgta gacaaaatgt
    15421 tcgtcatatc tttagcaaca aggattttgt cattaaattt tccacgtccg tcttacaagc
    15481 tgataagaaa gaaattacga tgattaataa aaacgaaaac acgacactca ctcaaaccat
    15541 tgctacaata tttgaagaat acctaatgga aattttacct caacgctcag acactcttga
    15601 taaacaagaa ttaaaccaaa aatcagatag aaaagaaaaa gaattcccaa gaattaaact
    15661 taatggtcaa tgttatttcc cggggcgccc caaaaccgta tagtataccg acacattgct
    15721 gcacaatata ttaatgatat ttatcagaat gttgattaca aaccccatca agatgattac
    15781 tcttcagctg aaaaatttct cactcacttc aacaaaaaat gcaaaaacca gactttggcg
    15841 ttgatttcca gccgtcctga ggggcgttgc gttgctgcct gcggtgattt cgggctagtt
    15901 atgaaagcat attttgacaa gatggaatca aatggcatca gtgttatggc agccatatta
    15961 ctggtggata accatgcttt gacggtccgg ctaagaataa agaacacaac tgaaggatgt
    16021 acccattacg tggtttcggt ttatgatcct aatgtaacta acgataaaat aagaattatg
    16081 agcgaaagca aagaggatat taaacactat tctctgatgg attttatgaa tgtagattat
    16141 agcctcctga aatggtcaaa tgatcatgtt attaaccaat ctgttgcaat aattccagca
    16201 cttccgaaag aacagctatt gatgttaaaa ggaactgtgg atgaaataac ccctccatta
    16261 tcaccagcaa cgatgaattt gctaatggca attggtcaga atcaccaact tacgcaactg
    16321 atgattcagc tccagaaaat gccagaacta catagaacag aaatgttgac tgcctataat
    16381 agtattaacc tccccggttt atatttggct ataaattatg gcaatgcgga tatcgttgag
    16441 actattttca attcattgtc agaaacagga tatgaagggt tactctcgaa aaaaaatctc
    16501 atgcatattc tggaggcaaa agataaaaat ggtttttctg gattattttt agcgatatca
    16561 cgtaaggata aaaatgttgt aacctcgatt ctgaacgtct tacctaaact ggccgcaaca
    16621 catcatttag ataatgaaca ggtgtataaa ttcctgagtg ccaaaaatag aacgtccagc
    16681 catgttttat accatgttat ggcgaatggt gatgccgaca tgctgaaaat ttttttggtc
    16741 gcgttacctt tgttaattcg cacatgtcat ttgactaaag aacaggtact cgatctcctg
    16801 aaggcaaagg atttttatgg ttgcccaaga ctatacctgg cgatgcaaaa tggacatagc
    16861 gatatcgtga aagttattct cgaagcattg ccctgtctgg cccaggaaat taacatttca
    16921 gcttccgata ttgtagatct tctgaccgct aaaagtcttg cgcgcgacac gggtttgttt
    16981 atggccatgc agcgcggaca tatgaacgtt attaatacta tttttaacgc attacccact
    17041 ctttttaata cgtttaaatt cgataaaaaa atatgaagcc cctcctcctg gcaaataatt
    17101 ctaatgaata tcccggtttg ttttcagcga tacagcataa acaacaaaac gttgtagaga
    17161 cggtttatct tgctttatct gaccatgcac gcctgtttgg atttaccgct gaagatatta
    17221 tggatttttg gcaacacaaa gcgccacaaa aatactctgc ctttgagttg gcttttgaat
    17281 ttggtcaccg ggttattgct gaattaatcc ttaatacatt aaataagatg gctgaaagct
    17341 ttggctttac ggataaccct cgatacattg cggagaaaaa ttatatggaa gctttactca
    17401 aaaaagcatc tccccatacc gtacgctaaa tctttgcctg cgccgatacg cataaacatc
    17461 ttccacatgc ccttcacgta tgcggttttg tagtgcgcgc cagtaatcag cgcggaacag
    17521 gtcggcgtgc atctcttcaa acagcggacc aatacgcggg tcggcgcata gccagtggcg
    17581 aaactcttcc gggaaaacat cgcccggcga gacgctgtac cacggttcgc tggcaagttc
    17641 gtcttccgga tagcgcggcg gcgggatgtc gcggaaattc acttccgtca tgtagcaaat
    17701 ttcatcgtaa tcataaaaaa ccacacgccc gtgacgggtg acaccgaagt ttttaaacag
    17761 catgtcgcca gggaaaatgt tagcagcggc aagctggcga atagcgttac cgtattcttc
    17821 aatggcgtcg cgcaactgct gaccttccac ttgttccagc cagatattga gcggcaccat
    17881 ccgccgctcg attatagaga tggcgaatta caatctgctc accaagatcg gtgatttttt
    17941 ccgctgcttc ctggagcagt aattccatta ataccgggga aatatgccgc ttctccagca
    18001 caaagttttc aaactcctgg gtgtccgcca ttcggcccac acggtcgtgc tctttcacca
    18061 gttgatagca ggcacgaacg tgagcggcag acatctcttt ctgcggcgcg aacttgtctt
    18121 tgatgacttt gaatacccga tcaaagcccg gcagcgtaaa caccaacatc accattccac
    18181 gaatacccgg cgcttcaatg aactgctcat tacagccctg tagataaacg agatattcgc
    18241 ggtagctttc ggttttggcg tgcttctggc agccgatagc catatacaat tcagcagtgg
    18301 ttttacctgg cagaatttcc cgtagccact cgaccagcgc tgcgggcagc ggtgcataaa
    18361 ccataaaata agaacgcgca aagccaaaaa caatgctcgc ttcggcggtc gtcgtcaggc
    18421 aggtatcaat aaataactcg ccgtcgtccg tctggtggat cggcagtaag aagggcaacg
    18481 tgccggaagg cgtgatcagt ttgcccacca gccaggcggc tttattgcgg taaaacagtt
    18541 cgttcgccac ctgtaaatga ctttccgcga ggttgtctgt ccccagcgtt tccgtcagat
    18601 ggcgaatgat gtaatgaatg tcacggcttt tattctgcca gcgcaggcgc agcggcaggt
    18661 cgctgataac gcgcatcagt agagattccc agccgtgatc ggggtgaaag tctttcgcca
    18721 gcgggcgggg aatggtacga aagcggcgct ctggctgaga gctaaagata aaaagccgct
    18781 cgggagtaag cgagcggtgg tcaaataacc gacagtacac ggagttaaaa aagctctccg
    18841 caatctcgaa gcgcgggtaa tccggcaaca gccgcgtgta atgctcttta acgcgcagta
    18901 aaaatgccgc gtccgtactt tggccgttag taatgcagcg cagttgctcc acgaccagac
    18961 caacgtgatg atcgtaaaga tggatacggt ttttcatcgc ctgctggaca gcatgccagt
    19021 cggcctgttc gaaacgctgc tgcgccccgg aagtcacttc gaggaatcga ccatactgag
    19081 cgtcgaagcc ttgcaaaatg gtttgagcaa tcagtaattc caggccacgc ggcatttagc
    19141 gccctcatca ggagcagaga attgccggat gcggcgtaaa cgccttatcc agcctacgtt
    19201 cggcaacggt tgtaggcatg ataagacgcg caagcgtcgc atcaggcatc gtgcaccgat
    19261 tgccggatgc ggcgtaaatg ccttatccgg cctacagtca gcaacgtttg taggttgctt
    19321 agaactgcga ttcttcagtg gagccagtca gcgcggtgac tgaagacgtg ccgccctgaa
    19381 taatagtcgt cactttatcg aagtaacctg tacccacttc ctgctggtga gatacgaagg
    19441 tatagccatc tttcgcggcg gcaaattccg gctgctgcac tttctcaaca tagtgcttca
    19501 taccttcgcc ctgcgcataa gcgtttgcca ggtcgaacat gttaaaccac atgctgtgga
    19561 tacctgctag ggtgatgaac tggaacttgt agcccatatc cgacagctgc tgctggaagc
    19621 tggcaatagt tttgtcgtcg aggtttttct gccagttgaa cgacggcgag cagttataag
    19681 ccagcagttt gcccggatat ttcgcgtgga tagcttgtgc aaagcgacgc gccagttcca
    19741 gatccggcgt ggaggtttca caccagacca ggtcagcata tggcgcatac gccaggccac
    19801 ggctgatcgc ttgctcaatg cccgcatgag tacggaagaa gccttcactg gtacgctcgc
    19861 cggtaataaa ttcgctgtca tacgggtcgc aatctgaggt gatcagatcc gccgcatcag
    19921 catcggtacg tgcaaccagc agggttggaa cgcccgtcac gtcagctgcc agacgcgccg
    19981 cgaccagttt ctgaatagct tcctgagttg gcactaaaac tttgccgccc atgtgaccgc
    20041 atttcttcac tgacgccagc tgatcttcga agtgaactgc cgcagcaccg gcttcaatca
    20101 tcgctttcat cagttcaaag gcattcagga caccgccaaa accggcttcc gcatcggcaa
    20161 cgatcggcag gaagtaatcg acatagcgcg gatcgcccgg ctcaatgccc acggaccatt
    20221 ggatctgatc ggcacgacgg aaggtgttgt tgatccgctc caccacagct ggcaccgagt
    20281 ttgccggata gagcgactga tccggataca tgctggccgc caggttagcg tccgccgcta
    20341 cctgccatcc cgacagatag actgcttcaa tacccgcttt cgcctgttgc agcgcctgac
    20401 cgccagtcag tgcaccgagg ttgttgatgt agcctttttt cgactcaccg tgcagcagac
    20461 gccacatttt cgctgcgccc agttgcgcca gcgtgcattc aggattgact gaaccgcgta
    20521 atttcaccac atcttccgca ctgtatgggc gagtaatgcc ttcccaacgc ggttgagtcc
    20581 actctttctg taattcttca atttgttgtg tacgggtttt catgtgcaga tgctccatag
    20641 ttatgtggtg gtttacgcta acaggcggta gcctggcagg gtcaggaaat caattaactc
    20701 atcggaagtg gtgatctgtt ccatcaagcg tgcggcatcg tcaaaacggc cctgggagaa
    20761 acgttcttcg cccagttcgc tggcaatgac tttcatctct tcgcccagca tctggcggaa
    20821 caaggctttg gtcaccggtt tgccattgct caacgttttt tgatgatgga tccactgcca
    20881 gatcgaggta cgggaaattt cagccgtcgc cgcatcttcc atcaggccat aaatcggcac
    20941 gcagccgttg ccggagatcc acgcttcgat gtactgcaca gccacgcgaa tgttagcgcg
    21001 cataccttct tcggtgcgtt caccatcaca aggtgccagc agctgatcgg cagtaatcgg
    21061 cgcgtcttgt tcgcgcaata cttcaagctg atttttacgg gagccgagaa tgtcgttgaa
    21121 taccgccatt gccgtgtccg caaggcctgg gtgagcgatc catgtgccat cgtgaccgtt
    21181 attggcttcc agcgctttat ccgcttttac tttgttgagc acctggttat tacgctcttc
    21241 atctttgctc ggaataaacg cggccatgcc gcccatcgca aaagcaccgc gtttatggca
    21301 ggttttaatc agcaggcgtg agtaagcatt caggaatggt ttatccatcg tcacggcctg
    21361 tctgtctggc aggacacgat cgggatagtt tttcaacgtt ttgatatagc tgaagatgta
    21421 atcccagcga ccgcagttca gaccaacaat atggtcgcgc agcgcgtgaa ggatttcgtc
    21481 catctggaac acggcgggca gcgtttcaat cagcaacgtc gccttgatgg tgccgcgcgg
    21541 cagattaaag cgatcttctg catagctgaa gacttcgctc caccaggccg cttcctgcta
    21601 ggactgggtt ttcggcagat agaaataggg accgctgccc tttgccaaca gtgcctgata
    21661 gttgtggaag aaatagagcg caaaatcaaa caggctaccg gggattgcct caccacgcca
    21721 ggtgacatgt ttttccggca agtgcagacc gcgtacccga caaatcaaaa ccgctggatt
    21781 gggcttgagc tgataaattt tgcctgcttc attggtatag ctgatggtac cgttaaccgc
    21841 atcgcgcagg ttaatttgcc cgtcgatcac tttgttccag tctggtgcca gtgaatcttc
    21901 gaaatcggcc ataaagactt tcacattggc gttcagtgcg ttgatcacca tcttgcgctc
    21961 taccgggcca gttatctcta cgcggcggtc ttctaagtcc gcaggaatcc cgcgaatttt
    22021 ccaatcagca tcgcgaatgg aagccgtttc cgaaataaaa tcaggcaacg ttccgttatc
    22081 aatatcttgc tgctgctgaa tgcgcgctgc cagaagttta ttgcgttgtg gcgtaaaatg
    22141 cgtcaccagc tcagtcagaa attctaccgc ttcggcagta agaatttgct tctcctgctc
    22201 gccatacggc cttgtgaaag ccagttcatc ggttgttgtt gcctgttcag tcatcgtgca
    22261 gctcctcgtc atggatccga aaacttcccc tctgtgaacg aaggaacgtt gtgcgctttt
    22321 cgttcagcac aactaagact actcatttaa aatgcaaaat caaaaacaat ttccatttta
    22381 attaaaaata cttgataact tattgataac aatatgaata aaatttattc acttgctgag
    22441 gtgcgtttcg gaaataaaaa aggcacccga aggtgcctga ggtaaggtgc tgaatcgctt
    22501 aacgatcgac tatcacagaa gattaatcca gcgttggatt catgtgccgt agatcgtatg
    22561 gcgtgatctg atagacgtaa tagttgagcc agttggtaaa cagcaaatta ccgtgactac
    22621 gccagctcgc tcgcggtttg ttttgcggat cgttgtgcgg gaaatagtta tacggtacat
    22681 ccgggtctag tccggcttcc acatcgcgta aatattcctg cgccagcgtt tgcgcatcat
    22741 attcgggatg gcccgtcaca aaggcaatgc gcttatcttt gctggcaaac agatatgcat
    22801 ccccttcttc cgtctctgcc agaatttcca gatcggtgta atcacgaatc aacgctgccg
    22861 gaaagtcagc atagcgcgaa tgcggtgcca ggaatgaatc atcaaagcca cgcgtcagaa
    22921 gcgcatgagg atggagaata tgatgctcgt aaacgccaga gagtttgtcg gtgcgagttt
    22981 gcttaggaat gccgtagagg atattgagcg cggcctgtac cgcccagcag acaaacagcg
    23041 tcgaggtgac gtgatctttc gaccactcca gcacctgttt gatctgcggc cagtaagcga
    23101 catcattaaa ctccaccagg cccagcggcg caccggttac aatcagaccg tcaaagttct
    23161 gttcctgaat atcttcaaag ttacagtaga agttgttcag atgctctgcg ggcgtgttgc
    23221 gcgattcacg ggaatcgatg cgcaacagct gaatatcgac ctgcaaaggt gagtttgaaa
    23281 gcaggcgcag aaactgattt tcagtttcaa tcttcttcgg catcaggtta aggatcagaa
    23341 ccttaagtgg acgaatttcc tgaccagacg cacgagaagt tgtcatcaca aagacgtttt
    23401 cttcacgcaa gaaattgacg gcgggtagct cgtccggcac acgaatcggc ataacctgat
    23461 tacctcacta catacgctta tacgtttaga catccagata gctgaagata accagaaaat
    23521 ttgccaatgt cgagcctgca tgttgaaggt gagaaagttt caaggaaaag gctacgttag
    23581 aatataagaa tgacgaaaag gagagaaaat ggttattagt attcgccgct cacggcatga
    23641 ggaaggggag gaactcgttg cgatttggtg tcgatctgtc gatgccaccc acgatttttt
    23701 atcagcagag tatcgggccg agctggaaga gctggtgcgt tcctttctgc cggaagcacc
    23761 gttgtgggtc gcggttaatg aacgggagca gccggttggg tttatgttgt taagtgggca
    23821 gcatatggat gcgctgttta tcgatcctga tgtgtgcggc tgcggcgtgg gtcggatgct
    23881 ggtggagcat gcgctctcga tggcaccgga actgacaacc aacgttaatg agcaaaatga
    23941 gcaggcggtt gggttctata agaaggtggg ttttaaggtt acgggacgct ctgaggtgga
    24001 cgatttgggg aaaccgtatc cgttgctgaa tctggcgtat gtgggggaat aagccgcctc
    24061 tgccgtgcct gcccaagctg gagaagacg
//
LOCUS       DAJTSA010000058        23648 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000058 DAJTSA010000000
VERSION     DAJTSA010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.058"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..558
                     /locus_tag="OO369_002463"
     CDS             <1..558
                     /locus_tag="OO369_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR5982708.1"
                     /translation="QGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQV
                     IYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEVV
                     AQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGTTYYFNKNMSTFVDYKINLI
                     DKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(1047..1845)
                     /locus_tag="OO369_002464"
                     /pseudo
     CDS             complement(1047..1845)
                     /locus_tag="OO369_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(1855..2406)
                     /gene="ybcL"
                     /locus_tag="OO369_002465"
     CDS             complement(1855..2406)
                     /gene="ybcL"
                     /locus_tag="OO369_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR5982709.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(2575..2907)
                     /gene="emrE"
                     /locus_tag="OO369_002466"
     CDS             complement(2575..2907)
                     /gene="emrE"
                     /locus_tag="OO369_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR5982710.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(3252..3566)
                     /gene="fliE"
                     /locus_tag="OO369_002467"
     CDS             complement(3252..3566)
                     /gene="fliE"
                     /locus_tag="OO369_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR5982711.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            3780..5439
                     /gene="fliF"
                     /locus_tag="OO369_002468"
                     /pseudo
     CDS             3780..5439
                     /gene="fliF"
                     /locus_tag="OO369_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            5432..6427
                     /gene="fliG"
                     /locus_tag="OO369_002469"
     CDS             5432..6427
                     /gene="fliG"
                     /locus_tag="OO369_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR5982712.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLRRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            6420..7106
                     /gene="fliH"
                     /locus_tag="OO369_002470"
     CDS             6420..7106
                     /gene="fliH"
                     /locus_tag="OO369_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR5982713.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            7106..8479
                     /gene="fliI"
                     /locus_tag="OO369_002471"
     CDS             7106..8479
                     /gene="fliI"
                     /locus_tag="OO369_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR5982714.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            8501..8842
                     /gene="fliJ"
                     /locus_tag="OO369_002472"
                     /pseudo
     CDS             8501..8842
                     /gene="fliJ"
                     /locus_tag="OO369_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            8839..9966
                     /gene="fliK"
                     /locus_tag="OO369_002473"
     CDS             8839..9966
                     /gene="fliK"
                     /locus_tag="OO369_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR5982715.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            10071..10535
                     /gene="fliL"
                     /locus_tag="OO369_002474"
     CDS             10071..10535
                     /gene="fliL"
                     /locus_tag="OO369_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR5982716.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            10540..11544
                     /gene="fliM"
                     /locus_tag="OO369_002475"
     CDS             10540..11544
                     /gene="fliM"
                     /locus_tag="OO369_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR5982717.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            11541..11954
                     /gene="fliN"
                     /locus_tag="OO369_002476"
     CDS             11541..11954
                     /gene="fliN"
                     /locus_tag="OO369_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR5982718.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            11957..12322
                     /gene="fliO"
                     /locus_tag="OO369_002477"
     CDS             11957..12322
                     /gene="fliO"
                     /locus_tag="OO369_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR5982719.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            12322..13059
                     /gene="fliP"
                     /locus_tag="OO369_002478"
     CDS             12322..13059
                     /gene="fliP"
                     /locus_tag="OO369_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCR5982720.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            13069..13338
                     /gene="fliQ"
                     /locus_tag="OO369_002479"
     CDS             13069..13338
                     /gene="fliQ"
                     /locus_tag="OO369_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR5982721.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            13347..14132
                     /gene="fliR"
                     /locus_tag="OO369_002480"
     CDS             13347..14132
                     /gene="fliR"
                     /locus_tag="OO369_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR5982722.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            14417..15040
                     /gene="rcsA"
                     /locus_tag="OO369_002481"
     CDS             14417..15040
                     /gene="rcsA"
                     /locus_tag="OO369_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR5982723.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(15084..15272)
                     /gene="dsrB"
                     /locus_tag="OO369_002482"
     CDS             complement(15084..15272)
                     /gene="dsrB"
                     /locus_tag="OO369_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR5982724.1"
                     /translation="MKVNDQVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            15435..15662
                     /gene="yodD"
                     /locus_tag="OO369_002483"
     CDS             15435..15662
                     /gene="yodD"
                     /locus_tag="OO369_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR5982725.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            15960..16775
                     /locus_tag="OO369_002484"
                     /pseudo
     CDS             15960..16775
                     /locus_tag="OO369_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(16772..18466)
                     /gene="yedQ"
                     /locus_tag="OO369_002485"
                     /pseudo
     CDS             complement(16772..18466)
                     /gene="yedQ"
                     /locus_tag="OO369_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(18637..18819)
                     /locus_tag="OO369_002486"
     CDS             complement(18637..18819)
                     /locus_tag="OO369_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR5982726.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(18898..19815)
                     /locus_tag="OO369_002487"
     CDS             complement(18898..19815)
                     /locus_tag="OO369_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR5982727.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            19988..20908
                     /gene="yedA"
                     /locus_tag="OO369_002488"
     CDS             19988..20908
                     /gene="yedA"
                     /locus_tag="OO369_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR5982728.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(20897..21367)
                     /gene="vsr"
                     /locus_tag="OO369_002489"
     CDS             complement(20897..21367)
                     /gene="vsr"
                     /locus_tag="OO369_002489"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR5982729.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(21348..22766)
                     /gene="dcm"
                     /locus_tag="OO369_002490"
     CDS             complement(21348..22766)
                     /gene="dcm"
                     /locus_tag="OO369_002490"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR5982730.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(22833..23373)
                     /locus_tag="OO369_002491"
                     /pseudo
     CDS             complement(22833..23373)
                     /locus_tag="OO369_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(23413..>23648)
                     /locus_tag="OO369_002492"
     CDS             complement(23413..>23648)
                     /locus_tag="OO369_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5982731.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         5889 a         6125 c         5899 g         5735 t
ORIGIN      
        1 cagggtaaaa atgaccgcac tgacgtaact gaagctaacg gtgatggttt cggtttctcc
       61 actacttatg agtatgaagg attcggtgta ggtgcaacct atgctaaatc tgaccgcact
      121 aatgatcagg ttatctacgg taacaacagc ctgaatgcat ctggtcaaaa tgctgaagta
      181 tgggcagctg gtctgaaata tgatgcgaac aacatctatc tggctaccac ctattctgaa
      241 acccagaaca tgactgtttt tggtaataac catattgcca acaaagcaca aaacttcgaa
      301 gtagttgcac aatatcagtt cgacttcggt ctgcgtccgt ccgttgctta cctgcaatct
      361 aaaggaaaag acttgggtgc gtggggtgat caggacctga ttgaatatat tgatgtaggt
      421 acaacctatt acttcaacaa aaatatgtcc acttttgttg attacaaaat caacctgatt
      481 gataagagcg atttcacgaa agcatctggc gttgctaccg atgatatcgt tgctgtagga
      541 ttggtttacc agttctaatc tgattactca aagatatgtt gtgggaggct ttgcctcccc
      601 aacatataag tggcgctctc aagccacttt ccttagaagc actatcttgc ttctgactat
      661 ataaattacc ctttattttc tgggacgttt caacgcccca tttttaataa tactcagtga
      721 acaattaacg tatcaattag atttaataac aacgatatcc gtacctaacc tgatatctaa
      781 tgtcataagc atccctttaa ttatcccctc aacctcttgt aaatttccca ctcttgagct
      841 ccacatagcc caaacgccgg atgcaaaaaa aatcatacgt gcatctctga aattgtgatg
      901 gaggaagtga gtatcaatta tatgtgctta taaacgttcc cgattggcac agaagtcacc
      961 ggattgttca ggctccaata ggttgagtat gtcgtattta ttttccaaaa taaaaatcga
     1021 tgctgttgct ccttaataaa atacttttac tctctggacg cttttcttag ctctcttggt
     1081 gttatattaa aacgattatg gaacctttca gtaaaacgag aaggacactt gtaaccattt
     1141 tccctggcaa tctcgctgat gggttttact gtcgtttgta tagcaggaca acgcattatt
     1201 taacctcacg tcgtccaata tactttgaaa acttattccc tcgcttgcca gacggcgatg
     1261 caatgtggaa acagaaatgt agagatatct cgctaccttg tttgctgtcc attttgttct
     1321 aggttcggat agcattaggt tataacaacg acttattaat gattgtttac tatctgataa
     1381 aagtaaacaa ttaacatgat gtgctcctaa tgaaagtaga acgcccattg ctaagtgttc
     1441 ctgaatttgc gttgagaagc ctcgggaaat agatgttttt agttgctccc aacaatatat
     1501 taactcgggg ttctgaggta agcagaaacc tgtcgtgtta cgaatttgat cagttatcgc
     1561 ataaaatttt tgaaactttt caattagatc aatgggataa taaagcattt ctgcaagata
     1621 aagacctgct tctggataat tcacaatata aaattcatat ccacagggaa ataatattat
     1681 ctgattatta tcaacagtta gactatgcgt ctcccaattg ataacttttt ttccctgacg
     1741 gatacgacat aaagctggca taagaggctt aaccctgtga atctcatgat gtttatgcat
     1801 ccgtatttct tcaatcttta ggttagactt atctcttgcc agcatactct taccctattt
     1861 tatctcataa actggtgtta tctcagcggt tgcaatttta ttggcattaa gcatataacc
     1921 aactaacgct ccgctggaat tagaatccac aggaatctta tcagttttta gagcccatac
     1981 tttaaactgg taatgatggg gtttatctcc tttgggagga catgctccac caaatcctgc
     2041 ataaccaaaa tcatttcggc cttgaacagc accagtcgga agttttgtac catcacgtct
     2101 tcctgcatca gtgggcaaat atgttacagt tgctggaata ttagcaacag tccagtgcca
     2161 ccatccactg cctgtaggag catctggatc atacacagtt acggcaaagc ttttggtgcc
     2221 ttcaggagca ccagaccagg ttaatgaggg cgatgtatta ccaccttcac acccaaatcc
     2281 agaaaataca tgagacgttg taagttgctc tcctgttttt atttcattac tagtgacctg
     2341 aaatgctgcg gcctgcgcag aaaatgttat gaatgccaat acacttgaaa cgataagttt
     2401 tttcataaaa acctcattgt tgtgacctat cgttatttta tttgatgtgc cttgatctca
     2461 ttatgcataa aggcgcaatg atcgcgcaaa tgcaatcata attataaagg tgccccgaac
     2521 attagccata atatgcacaa atcagattcc tgttatctca aggagaatta ttttttaatg
     2581 cggtgcactt cgtgacaaca agttaatgac taacacaccg gcacaaatca acatcatgcc
     2641 tatgatagct ggcaggtcca gtcgttgacc aaaaaatccc caggaaagca aactaatcag
     2701 aacgataccg actcctgacc agatagcata agcaatccct gtaggtatat aagccagcgt
     2761 ctgagctaat aaccagaatg atgcacaata gcaaataatt gtgccaacag agggccataa
     2821 ccgtgtaaac ccctctgaaa acttcattaa ggttgtacca atgacctctg caagtattgc
     2881 gccaccaaga taaatataag ggttcatagc atattctttc ctgttcatac tggagagaat
     2941 tgtactacag tttgaacaca actcaccgtt ttcatcctgg tgtatgtgta tccaaggggc
     3001 tctggcgtgg caagagataa aggcaggaac aataatgatc agaaggaacc ccaggctaac
     3061 gaatgagttc actcttcctg ggagcaaagc gaagacaaaa catgttatcg cgtttacgga
     3121 tcgtggatgg cagaaaacaa ccaggatcag gtatccattt taaaccagaa attgagtgag
     3181 tttgccccat ccatgcccca cgctgtggga tcggatataa ttaaacaagc atagtcaaca
     3241 ggttacacaa cctacacctg catggacatc acttcctgat acgccgccac cagcttatta
     3301 cgcacctgaa tccccatttg catagaaact gaggcttttt gcatatcggt catcacatcg
     3361 tttagcgcca cgccgggttc accgagggtg aatttttctg cctgcgtgcg ggcgactgtt
     3421 tgtgtatcgc ttatgcgatc gagcgcggcg tgcagctgcc cggcaaaact aatggtcggt
     3481 tgcggcagtg attcctgcgc acgcgcactc atcgccgtag cctgtaactg gctgataacc
     3541 ccttcaatcc cctgtatcgc tgacattttc gtctcccgga taatttctgg taacaaagcc
     3601 taccagtaag tcaataagac aaaggcgcta aatagcaaca aaaaaactgg tttattggcg
     3661 gatagaaaaa acgaaagcac aaataatggg agcgtcaatt tttcgagtgt gctgacccga
     3721 gagtgagtct tgttccactt tgccaataac gccgtccata atcagccacg aagtgcgcga
     3781 tgaatgcgac tgcagcccag acaaaatctc ttgagtggct taatcgcctg cgtgcgaatc
     3841 cgaaaattcc attgattgtt accggttccg cggcagtggc ggtcatggtc gcactgatcc
     3901 tgtgggcgaa agcccccgac taccgcacat tattcagcaa tctttccgat caggatggtg
     3961 gcgcaattgt cagccaactg acgcaaatga atattcctta ccgcttcagc gaagccagcg
     4021 gcgctattga ggttccggca gataaagttc acgaactgcg cctggcgcaa caaggtttgc
     4081 ccaaaggcgg cgcggtcggt ttcgaactgc tcgatcagga aaagtttggt atcagccagt
     4141 tcagcgaaca ggtgaattat cagcgggcgc tggaaggcga gctttctcgt accatcgaaa
     4201 ctatcggccc ggtaaaaggg gcgcgcgtac atctggcaat gccgaaaccg tctttattcg
     4261 tccgtgaaca aaaatcccct tctgcatcgg tgacggtaaa tctgttaccc ggccgcgcac
     4321 tcgatgaagg gcaaattagc gctattgtac atctggtttc cagcgcggtt gctggtctgc
     4381 cgcctggaaa cgtcacgctg gtggatcagg gcgggcatct gttaacccag tccaatacca
     4441 gcgggcgcga tcttaatgac gctcagttga aatatgccag cgatgtcgaa ggccgtattc
     4501 agcagcgtat tgaagcgatc ctgtcaccta ttgttggtaa cggtaatatt cacgcctagg
     4561 tcacggcgca actggacttc gccagtaaag aacaaacgga agaacagtat cgccctaacg
     4621 gtgatgaatc tcatgcggcg cttcgttcac gccagcttaa tgagagcgag caaagcggtt
     4681 ccggttatcc gggcggcgta ccgggggcgt tgtcgaatca accggcacct gcgaataacg
     4741 cgccaatcag cacgcctccg acaaatcaaa ataaccgcca gcagcaggcg agcaccacca
     4801 gcaatagtgg gccgcgtagc acacagcgga atgaaaccag taactacgaa gtcgatcgca
     4861 ccattcgtca taccaaaatg aacgtgggcg atgtgcaacg tctgtcagtc gcggtagtgg
     4921 tgaattacaa aaccttgcca gacggtaaac cattgcctct cagcaacgaa cagatgaagc
     4981 aaattgaagc tctgacccgc gaggcgatgg gcttttctga aaaacgcggt gattcgctca
     5041 atgttgttaa ctcgccgttc aatagcagtg acgaaagcgg cggagcactg ccgttttggc
     5101 aacagcaagt gtttatcgat cagttgctgg ctgccggtcg ctggttgctg gttgctggta
     5161 ctgctggtgg cgtggttgct gtggcggaaa gcggtacgcc cgcagctaac acgtcgcgcc
     5221 gaggcggtga agactgtgca gcaacaggcg caggcccgcg aggaagtgga agatgcggtg
     5281 gaagtccgtc tgagcaaaga cgaacaactc caacaacggc gcgctaacca acgactgggg
     5341 gcagaagtca tgagccagcg tatccgtgaa atgtctgata acgatccgcg cgtggtggcg
     5401 ctggtcattc gccagtggat aaataacgat catgagtaac ctgacaggca ccgataaaag
     5461 cgtcatcctg ctgatgacca ttggcgaaga ccgggcggca gaggtgttca agcacctctc
     5521 ccagcgcgaa gtgcaaaccc tgagcgctgc aatggcgaac gtcacgcaga tctccaacaa
     5581 gcagctaacc gatgtgctgg cggagtttga gcaagaagct gaacagtttg ctgcactgaa
     5641 tatcaacgcc aacgattatc tgcgttcggt attggtcaaa gctctgggtg aagaacgtgc
     5701 cgccagcctg ctggaagata ttctcgaaac ccgcgatacc gccagcggta ttgaaacgct
     5761 caactttatg gagccgcaga gcgccgccga tctgattcgc gatgagcatc cgcaaattat
     5821 cgccaccatt ctggtgcatc tgaggcgcgc ccaggccgcc gatattctgg cgttgttcga
     5881 tgaacgtctg cgccacgacg tgatgttgcg tatcgccacc tttggcggcg tgcagccagc
     5941 cgcgctggcc gaactgaccg aagtactgaa tggcttgctc gacggtcaga atctcaggcg
     6001 cagcaaaatg ggcggcgtga gaacggcagc cgaaattatc aacctgatga aaacgcagca
     6061 ggaagaagcc gttattaccg ccgtgcgtga attcgacggc gagctggcgc agaaaatcat
     6121 cgacgagatg ttcctgttcg agaatctggt ggatgtcgat gatcgcagca ttcagcgtct
     6181 gttgcaggaa gtggattccg aatcgctgct gatcgcgctg aaaggggccg agcagccact
     6241 gcgcgagaaa ttcctgcgca atatgtcgca gcgtgccgcc gatattctgc gcgacgatct
     6301 cgccaagcgt ggtccggtgc gtctgtcgca ggtcgaaaac gaacagaaag cgattctgct
     6361 gattgtgcgc cgtcttgccg aaactggcga gatggtgatt ggcagcggcg aggataccta
     6421 tgtctgataa tctgccgtgg aaaacctgga cgccggacga tctcgcgcca ccaccggcag
     6481 agtttgtgcc aatggtcgag tcggaagaaa ccattattga agaggccgaa cccagccttg
     6541 agcagcaact ggcgcaactg caaatgcagg cccacgagca gggttatcag gcggggattg
     6601 ccgaaggtcg ccagcaaggt cacgaacagg gctatcagga aggactggcc caggggctgg
     6661 aacaaggtct ggcagaggcg aaggctcaac aagcgccaat tcatgcccgg atgcagcaac
     6721 tggtcagcga atttcaaact acccttgatg cgcttgatag tgtgatagct tcgcgcctga
     6781 tgcagatggc gctggaggca gcacgtcagg tgatcggtca gacaccaacg gtggataact
     6841 cggcactgat caaacagatc caacagttgt tgcagcaaga accgttattc agcgggaaac
     6901 cacagctgcg cgtacacccg gatgatctgc aacgtgtgga tgatatgctc ggcgcaactt
     6961 taagtttgca tggctggcgc ttgcggggcg atcccaccct ccaccctggc ggatgtaaag
     7021 tctccgccga tgaaggcgat ctcgacgcca gtgtcgccac tcgctggcaa gaactctgcc
     7081 gtctggcagc accaggagtg gtgtaatgac cacgcgcctg actcgctggc taaccacgct
     7141 ggataacttt gaagccaaaa tggcgcagtt gcctgcggta cgtcgctacg ggcgattaac
     7201 ccgcgctacc gggctggtgc tggaagccac cggattacaa ttgccgctcg gcgcaacctg
     7261 tgtcattgag cgccagaacg gcagcgaaac gcacgaagta gaaagcgaag tcgttggctt
     7321 taacggtcaa cggctgtttt taatgccgct ggaggaagtg gaaggtgtcc tgcccggcgc
     7381 gcgtgtatat gccaaaaaca tttcggcaga agggctgcaa agcggcaagc agttgccgct
     7441 cggtccggcg ttattaggtc gtgttctgga cggtagcggt aaaccgctcg atggcctgcc
     7501 cttccccgat acgacggaaa ccggtgcgct gattaccccg ccatttaacc cgttgcaacg
     7561 tacaccgatt gaacatgtgc tggacaccgg cgtgcgccca atcaatgccc tgcttaccgt
     7621 cggacgcggg cagcgtatgg ggctgtttgc cgggtcgggc gttggtaaaa gtgtgctgct
     7681 ggggatgatg gcacgctaca cccgcgccga tgtcattgtc gtgggtttga ttggtgaacg
     7741 tgggcgcgaa gtaaaagatt ttattgagaa tatcctcggt gccgaaggac gtgcacgctc
     7801 agtggtgatt gccgctccgg cggatgtttc tccgctcctg cgaatgcaag gtgccgccta
     7861 tgccacgcgc attgccgaag attttcgcga tcgtggtcag catgtgttgc tgattatgga
     7921 ctccctcacc cgctacgcga tggcccagcg tgaaattgcg ctggcgattg gcgaaccacc
     7981 tgccactaaa ggttatccac cgtcggtgtt tgccaaatta ccggcactgg tcgagcgtgc
     8041 cggtaatggc attagcggcg gcggctcgat taccgcgttt tataccgtgc tcaccgaagg
     8101 cgatgaccag caggacccca ttgctgactc cgcgcgggca atcctcgacg gtcacattgt
     8161 gctgtctcgc cgactggcgg aagccgggca ctatccggct atcgatattg aagcgtcgat
     8221 cagtcgcgca atgacggcgt tgatcagtga gcaacattac gcgcgagtgc gcaccttcaa
     8281 acagctgttg tcgagttttc agcgtaaccg cgatctggtt agcgtcggcg cgtatgccaa
     8341 aggcagcgat ccgatgctcg ataaagccat cgccctgtgg ccgcagctgg agggctattt
     8401 gcaacaaggc atttttgaac gcgcggactg ggaagcgtct ctccaggggc tggagcgtat
     8461 tttcccgaca gtgtcataac ccaggagata acggcagatg gcggaagaac agctcaaaat
     8521 gttgatcgat tatcagaatg aatatcgcaa taacctcaat agcgatatga gtgccgggat
     8581 gaccagcaac cgctggatca actatcagca gtttatccag acgctggaaa aagccattac
     8641 tcagcatcgc cagcaactta atcagtggac gcagaaagtt gacattgccc tgaacagctg
     8701 gcgagaaaag aaacaacgtt tgcaggcctg gcagacactg caggaacggc aatccacggc
     8761 ggcactgctt gcagaaaacc gcctcgatca gaaaaagatg gatgagttcg cccagcgcgc
     8821 cgccatgagg aaacctgaat gattcgctta gcgcccttga ttaccgccga cgttgacacc
     8881 accacattgc ctggcggcaa agccagcgat gctgcacaag attttctcac gttgttgagc
     8941 gaagcattag caggcgagac aacaactgac aaagcggccc cccagttgct ggtagcaaca
     9001 gataagccca cgacaaaagg cgagccgctg gtcagcgata ttcttgccga tgcgcaacaa
     9061 gcggatttac tgatccctgt ggatgaaaca cttcctgtca tcaacgacga acaatccaca
     9121 tcaacaccgt taaccaccgc tcagacgatg acgttggctg cggtggctga caaaaatacg
     9181 acaaaagacg aaaaagcgga tgatcttaat gaagacgtca ccgcaagtct gagcgccctt
     9241 tttgcgatgt tgccgggttt tgacaatacg cccaaagtga ctgatgcacc gtcaaccgtg
     9301 ttaccggcag agaaaccaac gctcttcaca aaactgactt ctgcgcaact cactacagca
     9361 cagcctgatg acgcccccgg cacaccagct cagccattaa caccgctggt agcagaagcc
     9421 cagagtaaag cggaagtcat cagcacgcct tcgccggtga ccgctgacgc cagcccgcta
     9481 atcactccac accagacaca gccactgccc accgtcgccg cgcctgtttt gagtgcaccg
     9541 ctgggttctc acgaatggca acaatcattg agccagcata tttcgctgtt cacccgccag
     9601 gggcaacaaa gtgcagagtt gcgtctgcac ccacaggatt taggtgaagt gcaaatttcc
     9661 ctcaaagtgg atgataacca ggcgcagatc cagatgattt caccgcatca gcacgtacgc
     9721 gccgccctgg aagcagcgct gccggtactg cgtacgcagc tggccgaaag tggcattcag
     9781 ttagggcaaa gcaacattag tggcgaaagc tttagtggtc agcagcaggc cgcttcccag
     9841 caacagcaaa gccaacgcac agcaaaccat gaacctctgg cgggggaaga cgacgatacg
     9901 cttccggttc ccgtctcttt acaagggcgt gtaacaggta acagcggcgt tgatattttc
     9961 gcctaacgtc agaggtagca ccgtaatccg cgtcttttcc ccgctttgtt gcgctcaaga
    10021 cgcaggataa ttagccgata agcagtagcg acacaggaag accgcaacac atgactgatt
    10081 acgcgataag caagaaaagc aagcgatcgc tttggatccc gattctggta ttcattaccc
    10141 tcgcggcctg tgccagcgca ggttacagct actggcattc gcatcaggtt gccgctgacg
    10201 acaaagcgca gcaacgcgtc gtgccctccc cggtcttcta tgcgctggat acctttacgg
    10261 tcaatttggg cgatgcggat cgcgtgcttt atatcggcat aaccctgcga ctgaaagatg
    10321 aagcaacccg ctcgcggctg agtgagtatt tgccggaagt ccgtagtcgc ttgctgttac
    10381 tgttttcacg tcaggatgct gccgtactgg cgacagaaga aggcaagaaa aacctgattg
    10441 ccgagattaa aaccacactt tccaccccgc ttgttgccgg gcaaccgaaa caggatgtca
    10501 ccgacgtgct gtataccgct tttattctgc gataacgaca tgggcgatag tattctttct
    10561 caagctgaaa ttgatgcgct gttgaatggt gacagcgaag tcaaagacga accgacagcc
    10621 agtattagcg gcgaaagtga cattcgtccg tacgatccga atacccaacg acgggttgtg
    10681 cgcgaacgtt tgcaggcgct ggaaatcatt aatgagcgct ttgcccgcca ttttcgtatg
    10741 gggctgttca acctgctgcg ccgtagcccg gacattaccg tcggggccat ccgcattcag
    10801 ccgtaccatg aatttgcccg caacctgccg gtgccgacca acctgaacct aatccacctg
    10861 aaaccgctac gcggcacagg gctggtagtg ttctcgccga gtctggtgtt tatcgccgtg
    10921 gataacctgt ttggcggcga tggacgcttc ccgaccaaag tggaaggtcg cgagtttacc
    10981 catactgaac agcgcgtcat caaccgtatg ttgaagctgg cgctggaagg ctatagcgac
    11041 gcctggaaag cgattaatcc gctggaagtt gagtacgtgc gttcggaaat gcaggtggaa
    11101 tttaccaata tcaccacctc gccgaacgac attgtggtta acacgccgtt ccatgtggag
    11161 attggcaacc tgaccggcga atttaatatc tgcctgccat tcagcatgat cgagccgcta
    11221 cgggaattgt tggttaaccc gccgctggaa aactcgcgta atgaagatca gaactggcgc
    11281 gataacctgg tgcgccaggt gcagcattca cagctggaac tggtggccaa ctttgccgat
    11341 atctcgctac gcctgtcgca gattttaaaa ctgaaacccg gcgacgtcct gccgatagaa
    11401 aaacccgatc gcatcatcgc ccatgttgac ggcgtcccgg ttctgaccag tcagtacggt
    11461 actctcaacg gtcagtatgc gttacggata gaacatttga ttaacccgat tttaaattct
    11521 ctgaacgagg aacagcccaa atgagtgaca tgaataatcc ggccgatgac aacaacggcg
    11581 caatggacga tctgtgggct gaagcgttga gcgagcaaaa atcaaccagc gaaaaaagcg
    11641 ctgccgacgc ggtgttccag caatttggcg gtggtgatgt cagcggaacg ttgcaggata
    11701 tcgacctgat tatggatatt ccggtcaagc tgaccgtcga gctgggccgt acgcggatga
    11761 ccatcaaaga gctgttgcgt ctgacgcaag ggtcagtcgt ggcgctggac ggtctggcgg
    11821 gcgaaccgct ggatattctg atcaacggtt atttaatcgc ccagggcgaa gtggtggttg
    11881 ttgccgataa atatggcgtg cggatcaccg atatcattac cccgtccgag cggatgcgcc
    11941 gcctgagccg ttagtgatga ataaccatgc tactgtgcaa tcttccgcgc cggtttctgc
    12001 tgagccactg ctgcaggtga gcggcgcact catcgccatt attgccctga tcctcgctgc
    12061 tgcctggctg gtaaaacggc tgggatttgc ccctaaacgc actggcgtta acggtctgaa
    12121 aattagcgcc agtgcttcgc tgggcgcgcg tgaacgggtc gtggtggtcg atgtggaaga
    12181 tgcacggctg gtgctcggcg ttaccgcagg gcaaatcaat ctgctgcata aacttccccc
    12241 ttcagcacca acggaagaga taccgcagac cgattttcag tcggtcatga aaaatttgct
    12301 taagcgtggc gggagatcct gatgcgtcgt ttgttttctg tcgcaccggt ccttctctgg
    12361 ctgattacgc ccctcgcctt cgcgcaactg ccgggtatca ccagccagcc gctacctggc
    12421 ggtggacaaa gctggtcgct cccggtgcag acgctggtgt tcatcacctc gttgacgttc
    12481 attccagcga ttttactgat gatgaccagt ttcacccgca tcattattgt ttttggttta
    12541 ttgcgtaacg cgctgggaac gccctccgcg ccacctaacc aggtattgct ggggctggca
    12601 ctgtttttga ccttttttat tatgtcgcca gtgatcgaca aaatttatgt cgatgcgtac
    12661 cagccattca gcgaagagaa aatctcaatg caggaggcgc tggaaaaagg ggcgcagccg
    12721 ctgcgtgagt ttatgctgcg tcagacccgc gaggcagatt tagggttgtt tgccagactg
    12781 gcgaataccg gcccgttgca ggggcctgaa gctgtgccga tgcgcatttt gctcccggcc
    12841 tacgtgacca gcgagttgaa aaccgcattt cagataggct tcacgatttt catccctttt
    12901 ttgattatcg acctggtgat tgccagcgtg ttgatggcat tggggatgat gatggttccc
    12961 cccgccacca ttgctctgcc ctttaaactg atgctgtttg tactggtgga tggctggcaa
    13021 ttgctggtcg gttcgctggc gcagagcttt tacagctaga gaggcaaaat gacacctgaa
    13081 tcggtcatga tgatgggaac tgaagcgatg aaagtcgcgc tggcactggc agccccgctg
    13141 ttgctggtag cgctggtcac gggccttatc atcagtattt tgcaggcagc cacgcagatt
    13201 aacgaaatga cgctgtcgtt tattccgaaa atcatcgccg tatttatcgc catcattatt
    13261 gccggaccgt ggatgctcaa tctgttgctg gattacgtcc gcaccttgtt cactaacctg
    13321 ccgtatatca tcgggtagcc cgtacaatga tgcaggagac aagcgatcaa tggctttcct
    13381 ggttaagcct gtacttctgg ccgttactgc gcgtgctggc gctcatttct accgcgccaa
    13441 ttctgagcga acgcagcgtg ccgaaacggg taaaactggg tctggcaatg atgatcacgt
    13501 tcgccattgc cccatcatta cctgccaacg atgttcctgt tttttcgttc tttgctctgt
    13561 ggctggccgt acagcagatc ctgatcggca ttgcgcttgg ttttaccatg caatttgcct
    13621 ttgccgctgt gcgaaccgct ggcgaaatta tcggtctgca aatggggctg tcatttgcga
    13681 catttgtcga tcctggcagc catctcaata tgcccgtttt agcgcgcatc atggatatgc
    13741 tggcattact gctgttcctg acatttaacg gtcatttatg gttgatttca ctgctggtcg
    13801 atacctttca caccctgccg attggtagcg aaccgttgaa cagcaatgcg tttctggcac
    13861 ccaccaaagc agggagtttg attttcctta acgggctgat gctggcgtta ccgctcatta
    13921 ctctgttgct gaccctgaat ctggcattag gtttactgaa tcgtatggcc ccgcaattat
    13981 ccatttttgt tattggattt ccattaactc tgactgtcgg catctcttta atggcggcat
    14041 taatgccgtt aattgcgcct ttttgcgaac atttattcag tgaaattttt aatttgctgg
    14101 ctgatattat tagtgaatta ccattaatat aattccgtaa cgtttatcat gctaccctaa
    14161 ggattatccg aaaaataata tctgcgaaca tcttccagga tactcctgta gcgaaatatt
    14221 tgttttaagc tcactcacat atcgcaacat ttactttaag acaattccag gcaaattata
    14281 caacacttta cgggatagta agtccgcctg aaaaattgcg agagtggcgc attaggtgac
    14341 ccatgttgtt ccgtttagtc atgatgaaat attcaggtaa ggggaattat cgttacgcat
    14401 tgagtgaggg tatgccatgt caacgattat tatggattta tgtagttaca cccgactagg
    14461 tttaaccggg tatctgttga gtagaggggt taaaaaaaga gaaatcaacg acattgaaac
    14521 cgttgatgac cttgccatag cttgtgattc acagcgtcct tcagtggtgt ttattaatga
    14581 ggactgtttc atccacgatg cttctaacag tcagcgcatc aagcacatca ttaatcaaca
    14641 tcccaatacg ttatttatcg tttttatggc aattgccaat gttcattttg atgaatatct
    14701 attggtcaga aaaaatttat tgatcagttc taaatcgatt aaaccggaat ctctcgacga
    14761 tatccttggc gatattctga aaaaagagac aacgataacc tcgtttttaa atatgccgac
    14821 gttatcattg agccgaaccg aatcgagtat gttgcgaatg tggatggcag gtcagggaac
    14881 cattcaaatc tccgaccaaa tgaatatcaa agccaagacc gtttcatcgc ataaaggtaa
    14941 tattaaacgt aagatcaaaa cgcataataa acaggttatc taccatgtcg tccgactgac
    15001 ggataatgtg actaatggta tttttgtcaa catgcgctaa cacattctga ctggtggttt
    15061 cccaccagtc aggctgaata agattactct gctttctcaa caaagatacc gtcctgatgc
    15121 cctgcttcat taaagaacca gatgccgagc gggtagtctt ccagcgcaac caggtacatt
    15181 gtgccttcac taaactcctc aactgccagt accactccag gacgacgcgg accgccatcc
    15241 gttttgactg ttacctgatc attcaccttc atcgttttcc tcctgtggct ttgtgccagt
    15301 gtagaacaat ttcgtttttc tggcagcgcc aagcgcgcgc gagtgctgat tttctcgacg
    15361 gtctatactt aagagatgcc aggcggactt aacgactggc ggcaacaaca gagtaacggt
    15421 tgcgaggaaa gatgatgaaa accgcaaaag tgtacagcga taccgcaaaa cgtgaggtca
    15481 gcgtcgatgt cgatgccctg ctggcggcga tcaatgaaat tagcgaaagc gaagttcatc
    15541 gcagccagaa cgattctgaa cacgttagcg tcgatggacg tgaatatcat acatggcgtg
    15601 aattggcgga tgccttcgaa ctggatattc atgacttcag cgtctctgaa gtgaatcgtt
    15661 gaatgcacaa taaaaaaatc ccgaccctga gggtgtcggg atgaaacttg cttaagcaag
    15721 aagcacttaa aaaattcgtt acaccaggaa atctgatgtg ttcatcacct tatctgcaat
    15781 ttttttcgct gacaagaaaa tattcgcgct atgaatgatt aacgctactt atgaatagaa
    15841 atgtgacgcg cgtcacccct tggtggctct ggtgtcaacg ggatcgctcc cgaaaaaatc
    15901 atctttgatt atttgtgctg ttttgagaat catcctggta aggggagtac tgcgcgacca
    15961 tgttttcaat tcaacaacca ctactggttt ttagcgatct tgatggcacc ctgctggaca
    16021 gtcatagtta tgactggcaa ccggcagccc cctggctcag ccgtttacac gaagccaata
    16081 ttcccgtcat tctctgtagc agtaaaactt tagcggaaat gctgtacttg caaaaaatgt
    16141 tggggctaca aggcttaccg ctgattgcag agaatggcgc agtgatccag cttgctgaac
    16201 aatggcagga tatagacggt tttccacgca tcatctcagg tattagccat ggcgaaatct
    16261 gccaggtttt aaatacgcta cgcgagaaag aacattttaa attcacgact tttgatgatg
    16321 tcgacgatgc aaccattgcc gaatggacgg gattaagccg tagctaggcg gcgctgacgc
    16381 agctgcatga ggcgtcggta acgctaatct ggcgcgacag tgacgagcgt atggcacaat
    16441 ttatcgctcg tctgaacgaa ctgggcttgc agtttatgca gggtgcgcgc ttctggcacg
    16501 tactggatgc ctctgccgga aaagatcagg ctgccaactg gattatcgcg acctatcaac
    16561 aattgtcagg cagacgccca accacacttg gcctgggcga tgggccaaac gatgcgcctt
    16621 tactggaggt aatggattac gcggtgattg tgaaagggct aaaccgtgaa ggggtacatc
    16681 tgcatgatga ggatccggcc cgcgtctggc gaacgcagcg tgaaggaccg gaaggctggc
    16741 gtgaagggct ggaccatttt ttctccgccc gttaagcgtt atcgctcgcg aatacccgat
    16801 tgcgcccggc ctgttttgcg aggtaaagcc gacggtcggc caatgactgg agttgttcaa
    16861 aatcataatc accggtttcc tcgctgctac ttacccccag cgaggcactg atgcgtatcg
    16921 ttgtactctt ggcgatcagc atctcttttt catttaactt caggcgaata cgttctgcga
    16981 cctccgcagc ctgcgtcaga ttcgcgcctg gcagaatcac acaaaactcc tcaccaccga
    17041 cccgcccggc aacgtcctgc gcacgcaagg aactgctaat taatccggca gcatgagaaa
    17101 gaacacggtc gcccgcctga tgaccaaagc ggtcattaat cgctttaaaa tggtcaagat
    17161 cgacctgaat gacagaaaaa ggatgttggt gcgtctgaca caatttagcg agcggacggg
    17221 ctttttcgaa cagtgcgcca cggttatata aacgcgttaa ggtgtcgtgc cacgcctgcc
    17281 actgcaacga gctttgcaga acatacatgt tgctgaccat ccggcgaatc acataccagg
    17341 agaggagtaa catggtggta aagagcgccc acagcagggt taatgcaatg ctgatactgc
    17401 cgaaatcgcc gcgcacgcct tcgcttagcg tatggacgcg cgccagcaca ccgtcgaaat
    17461 ggtccagacg ttcccagcta acatagcgac tgtccatacg aatgccgccc cgcgtgtcat
    17521 gttccattgc ctgcgccagc aaggccagtt cacgaggatc aaaaatattc cctgttggat
    17581 ggtcaggatt ggaagaggtc aaaaatctca gcttactgtc atagagctga tactcaccat
    17641 cgaggttttt atcgatggcg tttcttaaaa attgctgcat agtacgcacg ggaatactca
    17701 tccccagcac gccataccag tagttattac tgtctaccgg aacactgacg gtaacctgcg
    17761 gttcagtatt gctggcgtgt tccggttgcg aagtgaacca gcgtaccgcg cggtgacgat
    17821 tttctcgttg cgaatggccg ataaaccagg gttgggtgac atagccgtaa taacgcgttg
    17881 gtacattgcg cgtaaacaag gtcggctgcg tcgaaacgta aaatccagca cgcgagacat
    17941 acatcgcctg ttcaaccatc gacgaggagt tgtgcgccag tcgcagcaag taaccaactt
    18001 ccagtgcagc ggtaatttca ttgtcgaggc tttcattttc gcgagacagg aaattcccct
    18061 cgctgactaa tgcatccgat acgccattga ctggcagggt gcgccgtcgg ttgagttcga
    18121 tttgccaggc gtgctcgtcg cgatgctgtt cgaactcggt tacggcatta cgcagagagg
    18181 tgaaatccag tggcgccacg agagcttcgc gcatgccatt acgcaaaaag atcagtttgt
    18241 cgacgttata ctgcaaatgc ttatcgagcg cgttggctac gttttccaga tgattacgct
    18301 agctggagat ataggcatct tccagcacca ccacttcacg ccaggtgagc aaggtggaaa
    18361 aaagcaatac cacgataaag cagagattaa cgacatgacc gggccccagg cggcgtgcga
    18421 gttttttcaa ccagctctgg ttttccattt ttgtctcgtg ctgcaccctg acgactccca
    18481 acctgctttt ttatgattct ggcataaccc ggcaatagcg taccgcaaaa cagcgataat
    18541 tttataatgg tggcatgata agacgcgact atcattcatt gcaggcattg ggacaccagg
    18601 ccggatgtga tccggcctgg tgttaatgat accctttcaa acttcttccg cagaacgact
    18661 cccctccccc ggaacaagat cggtttcatg aaaagcttcc cgcttgaccc cgtaaccgtc
    18721 ataccaacga cactcaacca taccgctgga gtatccagtg acaatcatcc gcgggccgcc
    18781 ctctttaacc gtaacttcct cactaaccat aaagctcata ctcgcctcct tttttcgagt
    18841 gaaacttgtt caccttagtt gaagatggcg aattttgcat aatagccagt gcgagtatta
    18901 atgtgcctga ccgcgcattt tcgctactgc tttcactccc agcaccacga tgccgccgat
    18961 gataaatcca agaatcagat ttaaaacagt cggtaatatc atcgccacca ctgcactttg
    19021 ctgcccggcg atatgttcaa tagcgtgatg cagcggcgca ataccatgta ccacaatccc
    19081 gccgccgacg aggaacatcg ccagcgtgcc gacaatcgat aatgctttca tcagccaagg
    19141 cgcgataatc aataaccctt tacctaatgc ctgcatcagc gcgctagatt tttccgccag
    19201 ccaataccct aggtcatcaa tcttaacgat aacccctacc agaccgtaga cgcccacggt
    19261 cactaccagc gcgatgcctg aaagcaccag cacctgatta agcaacggcg cctctgccac
    19321 aatccccagc gtgatggcga cgatttccgc agacaaaata aaatcggtac gaatcgcccc
    19381 tttaattttg tccttttcaa acttcagcgg atcctgcgcc gccagcttct ccagacgctg
    19441 ctggctctgc gctggatctt ctttatgttt acgcgcctcc agcatatgca gcactttctc
    19501 taccccttca aagcagagaa acgcgccacc aatcatcaac agtggcgtaa tcgcccacgg
    19561 gataaacgca ctgatgatca gcgccagcgg caccagaatc actttattga tcagcgatcc
    19621 tttcgccacg ccccagacca cgggaagttc ccggttggcc cgcacgcctg aaacttgttg
    19681 cgcattgagc gataaatcat cccctaatac accggcggtt ttcttcgccg ccagtttgcc
    19741 catcacagag atatcgtcca gcagtgtggc gatatcatcg agcaacgtca gtaaactact
    19801 cccggccaaa agcattccct ctcattttta ttgttgaagg gtgtaagtat gaagcaaaaa
    19861 cggcagctct gatacttgct tcacaggtca acatttattt aacaatcaag tgcaattata
    19921 tctctcgcca gcagaatgat tttcacgttt actatgaacc acctttttat ttccgccgtg
    19981 agggattatg cgtttccgcc agttgttacc gctttttggc gcgctgtttg cgttgtatat
    20041 catttggggc tcaacctatt ttgtcattcg gattggcgtg ggaagctggc ctccgttaat
    20101 gatggcgggc gttcgattcc aggcagccgg tattttattg ctggcatttt tgctactgcg
    20161 cggacacaaa cttcccccgc tacgtccgct gctcaatgcc gcgctgattg gcctgttatt
    20221 gctggctgtc ggtaatggca tggtgacggt tgccgaacat caaaatgttc cttccggcat
    20281 cgccgccgta gtgattgcaa ccgtgcccct ctttaccctg tgcttcagtc gcctgtttgg
    20341 cattaaaacg cgcaaactgg aatgggtggg tattgccatt gggcttgccg gaatcatcat
    20401 gctcaatagc ggtggaaatt taagcggcaa tccgtggggc gcgattctga ttttaatcgg
    20461 ctcgattagc tgggcgtttg gctcagttta tggctcgcgc attaccttac ctgtagggat
    20521 gatggcgggt gcgattgaga tgctggcggc aggcgtggtg ttaatgatcg catcgatgat
    20581 tgcgggtgaa aaactgacgg cgctcccttc cctgtcaggc ttccttgcgg tcggctatct
    20641 ggcgctgttt ggttcgatta tcgccattaa cgcttatatg tatttaatcc gtaatgtcag
    20701 tccggctctc gccaccagct acgcctacgt taacccggtg gtcgcggtct tgctgggtac
    20761 gggactgggt ggcgaaacgc tgtcgaagat tgaatggctg gcgctcggcg taattgtttt
    20821 cgcggtggta ctggtcacgt tgggaaaata tctcttcccg gccaaacccg ttgtcgcgcc
    20881 agttattcag gatgcatcaa gcgagtaaat gaatcccctg cgtgtcgatc tgcgcgctgg
    20941 caccttcgcc gcagatccac tcttccagac gctcggtaag cgccgcatcc gtcagcttct
    21001 cgcgcctacg taacgcacac tcccagacaa tcagtacgcg ccagccgagc tcctgcaagc
    21061 gactgatatc gcggcgatcg cgttcaacat ttttacctat cttctccagc cagaactcag
    21121 ttcgagtcgc aggcacttta aacagatagc agtgatgatg atgccagaag cagccgtggg
    21181 taaatatcac gcagcgatat tcatcaacga caaaatccgg gcgtccgggc agactggcgt
    21241 cctgaacgcg aaacgcaagg ccctgcccgg ttaacaaact ggcgaggcgc ttctcaatcg
    21301 ccgtatcacg cgtggcaatc gcgcgcatat ttttgctgcg ggtggcctta tcgtgaacgt
    21361 cgaccatgtt gtgcctcttg ctgacgcaac gccaccgcct gtttgatttt tggctcaagc
    21421 agttttgcca ccgcggcaaa gaccggcacg accaccgagt tgccgaactg gcgataggcc
    21481 tgagtgtccg aaaccggaat gcggaatttc gcttctcccg gcgcttcaaa acccattaag
    21541 cgcgcgcatt cacgaggcgt taaccgacgt ggacgatgtt gctgattcag cggatcgtca
    21601 aagtctttct cacccgtggc catatcccaa ccgcgatcga ttaaaatttc cgcgccatct
    21661 ttgtaataac gagcagacag cgtacgggtg acgctttgcg gattgttcgg ataaaccatt
    21721 ccataaccga agccgttacc gcgcgcctga tgttttttcg catatcgata gaggtacttc
    21781 cacagcaccg gcgtcaggat atatttcgcc tcaaccatcg ggtccagcag ctgcgccagc
    21841 gtcactcgct gtgcagggaa acattcgctg atatcacgca gagtaaaatc ggctttcaga
    21901 ttcagatcgc gacgaaaacc caccagcacg atgcgttcac ggtgctgcgg caggaaatgt
    21961 ttaccatcga tgattttcgg atcgtccggc ccgttatctt ctgcatcggc aacgtcatag
    22021 cccagttcgt ccagcgtctg catgatgatg cggaacgttt ttcccttatc gtgactcttc
    22081 aggtttttga cgttttcgag cacaaacatc gccggacgac gcgcgtcgat aatgcgcacc
    22141 acatcaaaga acagcgtgcc ctgggtatcg caggcaaaac cgtgcgcccg cccgagcgag
    22201 ttctttttcg atacgccagc cagcgaaaat ggctgacaag ggaaaccggc cagtaaaaca
    22261 tcgtgttcag gaatgtgttg acgaatatgt tccgccgccg cctcatcact cacgccttct
    22321 tgatgactga gggtgatgtc gcggatatct tcattaaaat gatgcgtcgc cgggtcgcaa
    22381 taatggttgg ctttataagt gcgtaccgca tgtttgttcc attcgctggt aaacacgcac
    22441 tgtccaccaa tcgattcaaa accacgacgg atgccgccaa ttccggcaaa tagatcgata
    22501 aagcgaaacg cataatgcgg atgatgttcc ggtggtttgg gtaacaacgt ttgcagatgg
    22561 gcaaactctt tctcacttaa acggtgccat gccgagtcat tcgccagcgc acgttttaaa
    22621 attgccgcgc tccagtgatt ctcacccaca ccattaagct gcgccaccag cattttaaca
    22681 tcataaattt gcagcagttt ctccagcatt gcctgtgcgg cattcccggt gctgtatgaa
    22741 tcggttactg atatattttc ctgcatagat ttaaccggcc atctgagatg gccgacaggt
    22801 taacataatt actgccgttt agggccacgc gctcagccag cgggtgaaaa cgcatctatc
    22861 accttgtgat cgataccttc attctccccc gccagttcgg caccgagctt cgccatcaaa
    22921 cagcgccaag ctcaatgccc ctgaaacggc aaatacgcgc gccaggccaa tcgcccccag
    22981 ggcttccagc cgatccgcat cctggacgat tttagcttcc gttgttaagg gggcgatttg
    23041 cgcgctgaaa ctgtgagcgg caatggcatg acaaacggcc tcaattttct ccgccggaaa
    23101 ttgcacgaac tcttcacgga gcaggcgacg cgtctcttct gccgccagga ttgaagaacg
    23161 ctgccgttgc ggatgatttt tcgccaggct cacgatatcg tgaaaataac aggcggttaa
    23221 aatcaccagc atatcgacat cgtcatctgc cgccagtttc tgtgccgtag cccagacacg
    23281 gcgaaaatga cacaggtcat gcgccgcgtc ctgatgttga tgatggtttt tcaaccagtt
    23341 ctcgaactat gcctgccagt gttgtaactc catacgcgct ccgttgccaa agagtctcaa
    23401 ccttagcagt ttttattcat agcgtctgca acgtgcggga cgaggtcggg tgtaccatgc
    23461 aatgccgcct aacaaggcac caaccaaggc cataaacaga aatccgacca atgctcctaa
    23521 ccacttcccc gcggtagatc ctaaatcact ttcaaatccc gacacggggg catcggggat
    23581 ccggctcatc acccagatgt atcgcgccat tgcccagacg aaatagccac accatgtata
    23641 gaaagccc
//
LOCUS       DAJTSA010000059        22935 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000059 DAJTSA010000000
VERSION     DAJTSA010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.059"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            86..862
                     /gene="exuR"
                     /locus_tag="OO369_002493"
     CDS             86..862
                     /gene="exuR"
                     /locus_tag="OO369_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR5982732.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1207..1869
                     /locus_tag="OO369_002494"
     CDS             1207..1869
                     /locus_tag="OO369_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5982733.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1873..2256
                     /gene="mzrA"
                     /locus_tag="OO369_002495"
     CDS             1873..2256
                     /gene="mzrA"
                     /locus_tag="OO369_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR5982734.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2403..2757
                     /locus_tag="OO369_002496"
                     /pseudo
     CDS             2403..2757
                     /locus_tag="OO369_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2795..3100
                     /locus_tag="OO369_002497"
     CDS             2795..3100
                     /locus_tag="OO369_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR5982735.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3103..3507
                     /locus_tag="OO369_002498"
     CDS             3103..3507
                     /locus_tag="OO369_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR5982736.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3497..3796
                     /locus_tag="OO369_002499"
     CDS             3497..3796
                     /locus_tag="OO369_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR5982737.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3982..4278
                     /locus_tag="OO369_002500"
     CDS             3982..4278
                     /locus_tag="OO369_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5982738.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITVYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4348..5334
                     /gene="yqjG"
                     /locus_tag="OO369_002501"
     CDS             4348..5334
                     /gene="yqjG"
                     /locus_tag="OO369_002501"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR5982739.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5627..5995
                     /locus_tag="OO369_002502"
     CDS             5627..5995
                     /locus_tag="OO369_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5982740.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="OO369_002503"
     CDS             complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="OO369_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR5982741.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7203..7904
                     /locus_tag="OO369_002504"
     CDS             7203..7904
                     /locus_tag="OO369_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR5982742.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7927..8091
                     /locus_tag="OO369_002505"
     CDS             7927..8091
                     /locus_tag="OO369_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5982743.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8303..9613)
                     /locus_tag="OO369_002506"
     CDS             complement(8303..9613)
                     /locus_tag="OO369_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR5982744.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9641..10972)
                     /locus_tag="OO369_002507"
     CDS             complement(9641..10972)
                     /locus_tag="OO369_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR5982745.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(11247..12611)
                     /gene="tdcG"
                     /locus_tag="OO369_002508"
     CDS             complement(11247..12611)
                     /gene="tdcG"
                     /locus_tag="OO369_002508"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5982746.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12683..13072)
                     /locus_tag="OO369_002509"
     CDS             complement(12683..13072)
                     /locus_tag="OO369_002509"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR5982747.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13086..15380)
                     /gene="tdcE"
                     /locus_tag="OO369_002510"
     CDS             complement(13086..15380)
                     /gene="tdcE"
                     /locus_tag="OO369_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR5982748.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15414..16622)
                     /gene="tdcD"
                     /locus_tag="OO369_002511"
     CDS             complement(15414..16622)
                     /gene="tdcD"
                     /locus_tag="OO369_002511"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR5982749.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16648..17979)
                     /gene="tdcC"
                     /locus_tag="OO369_002512"
     CDS             complement(16648..17979)
                     /gene="tdcC"
                     /locus_tag="OO369_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR5982750.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18001..18990)
                     /gene="tdcB"
                     /locus_tag="OO369_002513"
     CDS             complement(18001..18990)
                     /gene="tdcB"
                     /locus_tag="OO369_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR5982751.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19089..20027)
                     /gene="tdcA"
                     /locus_tag="OO369_002514"
     CDS             complement(19089..20027)
                     /gene="tdcA"
                     /locus_tag="OO369_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR5982752.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            20217..20561
                     /gene="tdcR"
                     /locus_tag="OO369_002515"
     CDS             20217..20561
                     /gene="tdcR"
                     /locus_tag="OO369_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR5982753.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            20817..21357
                     /locus_tag="OO369_002516"
                     /pseudo
     CDS             20817..21357
                     /locus_tag="OO369_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21379..21726
                     /locus_tag="OO369_002517"
     CDS             21379..21726
                     /locus_tag="OO369_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5982754.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            21895..22545
                     /locus_tag="OO369_002518"
     CDS             21895..22545
                     /locus_tag="OO369_002518"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5982755.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKTYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
BASE COUNT         6186 a         5782 c         5286 g         5681 t
ORIGIN      
        1 tcatcaaatt tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt
       61 ccgcattcca ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca
      121 acttgctgct gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact
      181 gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc
      241 catcatcatg ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt
      301 ggtttccaac cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta
      361 cggtccgttt gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc
      421 ggcaactcag gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg
      481 cggcgaacaa tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct
      541 ggcgacgcag aactccgccc tggcggccat cgttgaaaaa atgtggaccc agcgtagcca
      601 taacccgtac tggaaaaaac tacacgaaca cattgattcc cgtaccgttg ataactggtg
      661 tgatgaccac gatcaaattc tcaaggcgtt aattcgcaaa gatcctcatg ctgctaagct
      721 ggcaatgtgg cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga
      781 tttcgagttc aatgccgacc gctatctgtt cgccgaaaac ccggtagtgc atctcgatac
      841 ggccaccagc ggcagcaaat gaaattcctg tccgacaggc gctgcaatgc gcctgtttgt
      901 ctcgtcggta agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc
      961 ctgcaatcag caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc
     1021 tttgttacaa ttagattcaa tttgaattta tgcttttgaa tgctttctta tctcacgttt
     1081 taacagggaa tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc
     1141 aataacattg cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa
     1201 taatgaatgg aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc
     1261 ctggccaatc catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt
     1321 gaaaatggct tgcttccggc ggccttttta ccgggcgaca gtttactggt attggtcggc
     1381 gtgttgattg cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc
     1441 gccagcctcg gttgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc
     1501 gtacaaaact ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt
     1561 cataaacacg gtttatcggc gctgttaatt ggtcgcttta ttgcatttgt cagaacactg
     1621 ctgccgacga ttgccgggtt atcagggctg aataacgcgc gctttcagtt cttcaactgg
     1681 atgagcggtc tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg
     1741 ccggtatttt taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg
     1801 ctgctggtgt ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat
     1861 cgggggtaag ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact
     1921 gttttacttc tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct
     1981 acgctggcga ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg
     2041 catcaccttg acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg
     2101 ttaattacat ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca
     2161 ttgccccatg gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg
     2221 acccggttac gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca
     2281 ctcactttgg tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc
     2341 gcttcgttgt ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa
     2401 gaatgaaata ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg
     2461 ccactaccct gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa
     2521 aacaccaaaa ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca
     2581 actgttcaga tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg
     2641 aggtggcgga acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgatagaa
     2701 cggaaactgg cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc
     2761 acaatagtca ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca
     2821 tctgcgtgct gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg
     2881 cgagaagtcg aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca
     2941 gagccgttat cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc
     3001 ggcgcgtgct gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc
     3061 aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac
     3121 gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt
     3181 gaaatggtgg aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat
     3241 ctctttcaac ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg
     3301 agcctgatgg tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt
     3361 gccaccaccg tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa
     3421 tcgcgtaaat ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg
     3481 ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct
     3541 tagccagatc cagcaacaac ggctggatat ttccgccagt agtcgtgaat ggctggaggc
     3601 aacaggcgct tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt
     3661 tggcagtaga gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc
     3721 cagacgcggt tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca
     3781 acagcttcgc ggttaacttc cctctggccg gagcaattcc ggccttatcc atcaaatttt
     3841 ttgaatattt ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc
     3901 tccatcgaca gcaacgacgc taatagcgcg cccttgcaca aaaaaacaat cagcagcctg
     3961 agtggcgcag tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac
     4021 attttaatgc cgattctgtt tattaccgca ggctggggaa aaatcaccgt ttactctggt
     4081 acccaacaat atatggaagc aatgggtgtc ccgggtttta tgctgctgac ggcattttta
     4141 tttcacagca actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt
     4201 tctggcggat tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cggccgcctg
     4261 ctgaataaaa agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc
     4321 tcgtttttgc tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac
     4381 acctggtacg ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt
     4441 aactggctca ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag
     4501 aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc
     4561 atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg
     4621 ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat
     4681 caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt
     4741 actgttcccg tgctgtggga caaaaagaac cacaccatcg tcagcaacga atcagcagaa
     4801 atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac
     4861 ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac
     4921 aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg
     4981 aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc
     5041 ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca
     5101 gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat
     5161 ggcttcctgc gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac
     5221 atccgtaatc attacttccg cagccataag accatcaacc ctacggggat tatttcaatt
     5281 ggtccgtggc aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag
     5341 gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc
     5401 gcgaacgcct tatcaaacct acaaagcgtt tatattcaat atgttacaat gaccgcgtag
     5461 gccagataag cgtagcgcat ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc
     5521 acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt
     5581 aatttgctgc tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct
     5641 gaaagtacta aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag agtactggat
     5701 gtttattctg gtcaacatca tctttacgtt cgtgctgggg ctgctggata aaatgttagg
     5761 ctggcaacgc gctggtggcg aaggcatcct gacgataatc tacggtattc tggtgttttt
     5821 accgtggtgg gcggttcagt tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc
     5881 actgctattc ttaatcccgt ttatcggctg gcttattatc atcgtcttta actgccaggc
     5941 gggtacgcca ggcgaaaacc gctttggacc agatccaaaa ctggaacaag aataatgatt
     6001 aagcaccttg taaaaggtgc ttcaaattgt tgacaaagtg cgctttgttt atgccggatg
     6061 cggcgtaaac gccttatcca gcctacaaat tgccaaaatt caatatattg cagtgactgc
     6121 gtaggcctga taagcgtagc gcatcaggca gttttgcgtt tgtcagcagt cagaagcacc
     6181 ttgtgaaagg tgcttaatag attattttcc agcgaacaac tttggaattt cccgcagaca
     6241 ccaggatttc gcttccccca tactgtcacg acgccaggcc ataataatat cgatctcgct
     6301 ggtcgattcc gggctgacga cacgcaaccg cccttccgca atatcttttt cgaccatcgg
     6361 atacggcatc gtcgccacgc caagccccgc cagtaatgcc tgacgtttat cttcaatcgt
     6421 gctcaccgtt aagcgcggct gtttgtccag cagctgtacg gtcaacaccg ggcgctcacg
     6481 agcggtatcc gctaccgcaa ttccacgata tttcacacgc gtcacttcag ataacggttc
     6541 cggctcctga tgaatcgggt gatcaggcgc ggcaacgtag acgttcatta acgtatagag
     6601 cttgcgcgag ttgatctccg acgaggaacg aaaatgcata tccggcgcga taacgatatc
     6661 cgcccgcccc tgctccagcc gttcccacgc ccccgccagc acttctgtga tgattgccag
     6721 ttgggtattg gcttttgccg ccagtttgtc gattaacggg aaaaaggcag gtgtcggtac
     6781 cagcgcttcg gtcacaatgg tgagatgcgt ttcccaaccg cgcgcgagag cttccgcatc
     6841 agtagtcagt ttatctgcgg cttccagcaa aacgcgccct cgctccagca acatcctccc
     6901 gacattggtg aatttggtgc gatggcccga gcggtcaaac agcaccacat ccagctcttc
     6961 ttccagtttt tgcatggtgt agctaagtgc ggaaggcacg cgtcccagct catccgctgc
     7021 cgccgcaaaa ctgccccggc gatcgatcgc atccataacc cgtagtgctt ccagcgttaa
     7081 tgccctttct ttggccattt cgttctcatt caggaaattt gaacataccg ggcagaatat
     7141 ctggctaaca atgtagcgtc cagcccctta ccataaaagg aagtaaagag aggtcaagaa
     7201 ttatgattac tacccgaact gccaggcagt gtggacaagc agactacgga tggttgcagg
     7261 cccggtatac tttttccttt ggacactact tcgacccgaa attgttaggc tatgcctccc
     7321 tgcgtgtgct taaccaggaa gtgctggccc caggtgccgc ctttcagccg cgaacctatc
     7381 ccaaagtcga tattttaaat gtgattctgg atggagaagc agagtatcgc gacagcgaag
     7441 gcaatcatgt tcaggccagc gccggagagg cgttgctgct ctctacccag ccgggtgtca
     7501 gctatagcga acacaatctc agcaaagaca aaccgttaac gcgaatgcag ctttggctgg
     7561 atacctgccc gcagcgagag aatccgctga ttcaaaagct ggcgcttaat atggacaagc
     7621 agcaattaat cgcctcgcca gagggtgcga tgggaagcct gcaattacgc caacaagtgt
     7681 ggctgcacca tatcgtgctc gacaaaggcg aaagtgcgaa tttccagttg catgggccac
     7741 gcgcgtattt gcaatcgatt cacgggaaat ttcatgcgct tacgcatcat gaagagaaag
     7801 cggcgctaac ctgcggtgat ggggcgttta ttcgtgacga ggctaacatt acgctggttg
     7861 ccgattcccc actgcgcgct ttgctgatag atttgcctgt ctagttgttt ttacagggag
     7921 atgatgatga gtaaaaaatc gaccaaaaag cgccagccag cgaagcccgt ggtggcgaaa
     7981 gaacctgctc gcaccgccaa aaattttggc tatgaagaga tgttgagcga gctggaagct
     8041 atcgtcgcgg atgctgaaac gcgtttagcc gaggatgaag ctaccgcgta aagacaggtt
     8101 gccggatgcg gcgtaaacgc cttatccggc ctacaaaaca caacaacatc aacaaattaa
     8161 acaccccgta ggccggataa gatgcgccag catcgcatcc ggcatcaggt acaaaacaca
     8221 acaacatcaa caagttgagc accccgtagg ccggataagg tgcgccagca tcgcatccgg
     8281 cacaatactc aaaacctggc gtttatctgg ccttgctcgc cataatctcg ataatctgcc
     8341 gatccgtttg ctgcatcgaa tggcttgcta acgcacacag gttggcaatc gactgctcaa
     8401 catcatgcgc cacgatccct tcattgccgg tcacggcggt atcatccagc gccattaaca
     8461 ccgctttcca cgcagccgaa gcactggtcg aaaccttcat cgcgcagctg ttcgacgcac
     8521 catcgcaaat catgccgctg acatcgccga tcatactgct gatcgccatc gagatagttt
     8581 cataacgccc atccaccagc catgccatcc cggcggcggc ccccattgct gcggtcgttg
     8641 cggcacaaag tgcagacaaa cgcggtaact ggttatggat gtaaattgcg ctcaaatgag
     8701 aaagcatcag cgcacgcgcc agtcgttcat catccgctcc gaagtgttct gctacaacca
     8761 ccacaggcat tattgcagtg atcccctggt tacccgagcc ggagttactc atagccggaa
     8821 gcgtagcgcc gcccatacgc gcatcggatg ccgcgctggt acgaatcaca atggatgaag
     8881 agagatcttt cgccagcaaa ccgcgctcgc actgtttttc cagcgtcgcg ccaatatgca
     8941 gcccccactt accgctcaaa ccttcctgcg ataacgcaca atttagcttc gcggaatcga
     9001 gaataaagcg gatcgccgca aatgggactt cattgacgaa cttcaggatc tcagccagcg
     9061 tcgttctgga aagcaccgtc agcggcgact cttgctcgcc ctctgccaca cacgcctggt
     9121 gggtaaacac cacaccattg tgcgtctcaa tatgcacaat gttggtatgc ccgccgacga
     9181 tggtgacaca cgcccacttc tcaccgttcc agactttggc gcgtgagaag aggatttcat
     9241 tgcaaggttc ctggatctta acagagactt tccccgccgc cagcagtgct ttggcatcgg
     9301 taattgcctg cgcagttgcg tctttcagca cttccagccc ggcgttggca tttccaccta
     9361 acgcccccag cgccgccgca atcggcagcc ccaccattcc cgtgccggga acggtgacgc
     9421 ccagaccgtt cttcatcaga tttggcgaaa cccaggcttc tacacgttca accggacctt
     9481 ccagttctgc cgcagcaacc gccgccgcca gcgccagtga aatcggttca gtacatccca
     9541 gcgccggttt tacttcctcc tgaacggcga ggatgtaacg ctgccataac ggatttaaag
     9601 tcgaatcaaa catattaaaa accttaaaat ttcaggtaaa tcaagaaaat gccaggaaca
     9661 gagaaacaca aagcaacaaa ccagtgacga taatcaggta cagagacatc cctttgtatt
     9721 tgtgcaatgc cggtactttg taaaccagcc acgccgggat caggcaccct accatgccga
     9781 aaatcgggct acagatagag gtgaagctca acaccggtgc gttcagtacg atggcgctcc
     9841 aggccagcaa aatggcgaaa atcatgatgc cgcgctgaac aagattttcg ttaatcttct
     9901 cggcaggcat cttgcgacgc aggatgttca ttacgatccc ttgcgttgct tcgcgaaagc
     9961 ctaaatagac gccaaagaat gcagtcatga cagcaaagat attgagaatg acgctgacca
    10021 ctttcaccca cgctgcgccg tcaccgctaa taaactgtgc ggcaatcgcc agcgcggaaa
    10081 tattctgctc gtaggctttt accgcttcgt catgtcccat cgccagcgtg aacgacacgg
    10141 cgtagaaaaa gacggtgaca aacaaaatgc caaacgcgat attcattgcc cgcaatgctt
    10201 tatgccgcgc cacttcaatt gatttttccc gcgagcgata agagatcacc atcgggctta
    10261 acgtctggat aaacagaatc gacgtcaggg taaacggcag cgtaataatg gcgtttttca
    10321 ccagcagccc cagcggcggt agcgaaccga cgttatacag atgccacatt cccaccatcg
    10381 acacgcccag cgcagcgact accagcagct tggtcagcac catgtcggtc gaaattttga
    10441 ataacaattt ctcgccgcgt gaagagatcg ccaccagaat gcaaatcagt accagaccat
    10501 aaaagggact gtctgacagc aacccttccg tcacgccgaa ggtatgcaga taggaagcac
    10561 tatcgttggt gatggcggtg gaatagacga acatccaaat caccagcatt acgaaataga
    10621 gcgcacctaa caggatgccc cagtttttac ctaaataacc gctaatgaca ctcgggtaat
    10681 ctttacattc tggtgattct gccagcgtat taataaacaa ccgctgaaac agatacattg
    10741 ccgggtaacc aatcaccgat gagagcaaaa atacccacaa tcccatcaaa ccgacctgca
    10801 ccgggagaaa aacaatcccc gcgccaatcg ccatcccgat actcataatc acccagccgg
    10861 tgtcagtact gtcgaatttg atcgcttctc gccactcgct ttcactcatt ccagcacgac
    10921 ccgccggggt cgaagcgtct gcaatgacgc ctttattcga tgcaatttcc ataatttctc
    10981 gctcaatatt ttgtagggct tattattttt ttccgagccg catcaaggcg atatgcggtc
    11041 tacgtttttg caggcgagtt attagaataa agaaatgata cgcgcgaggt cagagaaaat
    11101 cttcacaatc aagactttaa aataacggag atggataaga attttctact aaattaatca
    11161 caagagaata ttcattctct atttgcggca gatcacaaaa aaaaggtgca catttgtgca
    11221 cccaaggatg aaagctgaca gcaatgtcag ccgcagacca ctttaatggc cagtcctccg
    11281 cgtgatgttt cgcggtattt atcgttcata tctttgccgg tttcatacat cgtctcgatc
    11341 actttatcga gtgaaacacg cggtgccgag gtgcggcgca tcgccatccg cgcggcgttt
    11401 actgctttca cggcattaat ggcattacgt tcaatgcacg ggatttgtac ctgtccggca
    11461 accggatcgc aggtcagccc aaggttatgc tccatcgcga tttccgccgc attgcatacc
    11521 tgcgccggac tgccgcccag tagttcagtt aaccctgccg ctgccattga acaggccacg
    11581 ccaatctccc cctgacagcc gacctccgcg ccagagatgg aggcgttcat tttatacagc
    11641 gcgccaatag cccccgctgc cagaaaatag cgggcaattg accgctcgtt taccggacga
    11701 cggaacttat cgtaataagc cagcactgcc ggaataatgc cgcacgcacc gttagtcggt
    11761 gccgttacca cgcgcccgcc agctgcgttt tcttcactaa ccgccagcgc gtacatgttg
    11821 atccagtcga tgacattcat cggatcgtta gagatgttat cgctagaaac cagctgacga
    11881 cgcagcgcta cggcacgaca cggcacattg agcggaccag gcagcacgcc ttcagtgttc
    11941 atcccgcgtt caataccgtc atgcatcact tgccagatac gggcaaaccc tgcgtcaatt
    12001 tccgctttgc tgcgcagcgc cagctcgttg tgcatcatca ggccagatat agacaggccg
    12061 ttgtaatcac acattttcag cagttcacct gctgagtgga aatcgtaagg tacggacgtt
    12121 tcgacatcgt gcgacaggcc gaagtgttct tcttcgacaa taaacccgcc gccgacagag
    12181 taataggttt tacttaatag cgcttcctgt cccttccagg cagtgatccg cattccgttc
    12241 tcatggcgag gcaacatttc gggatggaag ataatgttct ttgctacagg aaaatcaaca
    12301 atatgcgcac cagatgccac tggcagccgc ccgctgcgcg ttactaactc tataaatgca
    12361 gggatctcat caatgacaac atcctgcgga ctgtttcctg ccagtcccat gataatggcg
    12421 acatccgtgg catggccttt gcccgtcagt gacaacgacc cgtacagatc gaccacaata
    12481 tggctcgtcg cggttaataa gccgctactt tccagccgat caataaaact ttttccggca
    12541 ttcattggcc ccacggtatg cgaactggag ggaccaatcc caattttgaa aatatcgaat
    12601 gcactaatca tatccacacc ctcggattgc cgttcagtga agtggagcgg aacgaccttg
    12661 cgaccgtccc gctcacgagg ctttacgcac tacgtactgc gatagcttca atttccagct
    12721 tcacatcttt cggcaaacgc gcaacctgca cacagctccg ggtcgggtag gtcgcctgat
    12781 gctcatcgaa gaactgctta tagacttcat tgatggtggc aaaatcattc agatcggtga
    12841 tgaacacggt catcttgatg atatcgccca cgctcagccc ggcagcaacc acgatcgctt
    12901 tgacgttttc gaggcttaaa cgcgcctgat cttgcacatc agccgggatc tcaccggtct
    12961 gcgggcaaac cggaatttgc ccggaggtga agaccatgct gcctaaatca acgccctgaa
    13021 cataagggcc gattgcgcct ggggcacgtt gcgtttcgat aatctttttc atacatcctc
    13081 cggcgtcaga gcgcctgggt aaaagtacgt gaaataacat cctgttgctg ttcacgggtc
    13141 agtgcgttga agcgcacggc gtagccagag acacggattg tcaggttagg atatttttcc
    13201 gggtgctcga tggcatccag cagcatttcc cgattcatta cgttgacgtt gaggtgttga
    13261 ccgccttcga catccgcttc gtggtggaaa tacccatcca gcaggccgac aaggttggtt
    13321 ttacgtactg gatcttcttt gcccagcgcc gcaggaacga ttgagaaggt gtacgagatc
    13381 ccatctttgg cgtaggtgaa cggcagtttc gccaccgacg tcaatgaggc cacggcacct
    13441 ttgcggtcac gaccatgcat cgggttagcg cccggcgcga acggtgttcc ggcgcgacga
    13501 ccgtccggcg tattaccggt tttctggccg tacaccacgt tagaagtgat agtcagaatc
    13561 gactgggtag ggacggcgtt gcgataggtt ggcagcgctt taattttctt cataaagcgt
    13621 tcaaccaggt cgcaggcaat gctgtctacg cgctcgtcgt tgttgccgta ctgcggatat
    13681 tcaccgtcga tttcaaagtc caccgccagg ccgttttcgt cacggattgg tttcacgcgg
    13741 gcatatttga tggcagacag ggagtccgtc gccaccgaca ggcccgcgat gccgcatgcc
    13801 atagtgcgat agacatcacg atcgtgcagc gccatcagcg aagcttcgta gctgtacttg
    13861 tcgtgcatgt agtgaatgat attcagcgcg ctgatgtact gcaccgccag ccagtccatg
    13921 aagtgatcga ggctgtccat cactttgtcg taatccagca cgtcgtccat cagcggtgct
    13981 gttttcggcc cgacctgaat cttcagcttc tcgtccaccc cgccgttaat tgcgtagagc
    14041 agcgttttcg ccaggttagc gcgtgcacca aagaactgca tttgcttacc aatcaccatt
    14101 gggctgacgc agcaggcaat cgcgtaatcg tcgctgttga agtcagtacg catcagatcg
    14161 tcattttcat actgcaagga agaggtgacg atcgacacct gcgcggcata ttttttgaag
    14221 gcaatcggta attcttccga ccaaagaatg gtcaggttag gttccggtgc cggccccata
    14281 gtgtgcaggg tgtgcaaata gcggaaggag tttttggtca ccagcgtacg accgtccagc
    14341 cccatcccgc cgatcacttc cgtcgcccag attgggtcgc cggagaacag cgaatcaaat
    14401 tccggtgtac gcaggaagcg taccatacgg atcttcatga tgaagtgatc gatcagttcc
    14461 tgtgcctgct gctcattgag gacgccagct ttaaagtcgc gctcaatgta gatatccagg
    14521 aacgatgccg tgcgccccag cgacatcgcg ccgccatttt gcgatttcac tgccgccaga
    14581 taagcgaagt agagccactg caccgcttcc tgtgcattct gcgccgggcg agagatatca
    14641 aagccatatt tcgccgccat ttcctgaatc tgcaacagcg catgacgatg ctctgccagc
    14701 tcctcacgca gacggatggt ggcttccaga tcctcgcctt tttccagacg agactggaga
    14761 tcggcaaatt gcagttcgcg ttcacgcacc agataactga tgccatacag cgctacgcgg
    14821 cgatagtcac cgataatgcg cccacgacca tagccatctg gtaaaccggt cagcacgcca
    14881 gatttacggc agcgcagcat atccggtgag taaacatcaa atacgccctg gttatgggtt
    14941 ttacgcagat cggtaaacag atattcaaat tcactgtcca tttcacggcc ataggcgtgg
    15001 aatgaacttt taatcatatt aatgccaccg aacgggtgta gcgcacgttt caacggcgca
    15061 tccgtttgca ggccaacaat tttttccagc ggctggttaa tatatcccgc atcatgagcg
    15121 gtaattgtgg tggcaatatt ggtatcgaaa tcaaccggcg cgtgggttgc gttttcgata
    15181 cggatgcctt ccattacctt ttcccacaat tccgtggtgg caggcgtcgc ttcggcgaga
    15241 aaagattcat cgccttcata cggtgtatag ttatgttgaa taaaatcgcg gacattaatt
    15301 tcgcttttcc agtccgtacc tttaaagcca agccatgcgt cagcgtacag cttatcgctg
    15361 gtatcaatat ctaccttcat gaaaaataat ctctctacaa tacttcaact aaattatgca
    15421 aattctgcgg gcgcgttaac tttacctaaa tgaatggcat ccaaagcaat cattttttct
    15481 tcgttagtcg gaataacgac gcaaatgaca tgcgcatttt cactggaaac aattcgctcg
    15541 ccaaaagagt tagaacgatt attcatttct gtatcaatct ctacgcctaa tacagccaaa
    15601 tgttccatga ccagacgacg aattaaactt gaattctctc ctattccgcc ggtgaatata
    15661 attccatcca gccgacgtaa tgaagctgca tgtccggcaa tatgacgggc aattcggtga
    15721 acaaaggttt taattgccag ttgcgcgcgt tcgtgacctt catgccaggc tttttccaga
    15781 acacgtaaat ccgaagaaag accggaaata cctaataatc ccgactcttt attcactacg
    15841 cgttccaggt cacccaggct ctggttggtt tggctggcga cccaggacat cgcaccaaag
    15901 tcgacatcgc cactgcgggt acccatcatc aagccttcca gcggcgtcat tcccattgag
    15961 gtgtcaacac tctgaccgtt gcgaactgcg cagattgacg cgccattgcc aagatgcgcc
    16021 acaaccaggc cggagtcatc ttccgccaga ttcagcagcg aatgtgcgcg ctgggaaaca
    16081 tagcggtgcg acgtgccgtg gaaaccataa cggcgtacac ctaactcttc ataatatttc
    16141 cacggcaggc cgtataaata agcttccgga gccatcgtct ggtggaaact ggtatcaaat
    16201 accgccacct gagttacgcc cggaaataat tgctgcgccg attcaatacc acttaaattt
    16261 gcgtaattat gcaggggtgc cagtggagaa acgcgacgga tattatcaat gacttcatcg
    16321 gtaataatgg cggactcggt aaaaatactg ccgccgtgag cgatgcggtg gccaattaag
    16381 gccacgctgt catttaaatt ccgtttttcc agttcaaatg caattgcctt caatgcacct
    16441 tcgtagctgt ggtgagccag cggtgctggc tctcccccat ttacggataa gaatgcattt
    16501 tccgagttaa taccgtcggc aatacctgac attaatactt cacaatcgct ggcatcgagt
    16561 acggaaaact taatcgaaga cgaaccacag ttaataacca aaacaaccgg aaattcattc
    16621 atctcttttc tcatcctgag ttacggatta aaacagtttg tatacgatgt tcaggatggt
    16681 cagcagacca atcacggtaa caaacacgtt atccagacga ccacggtatt tcgccagaga
    16741 cggcgcttta cggatagcat acatcggcaa caggcacagc agggatgcga taatcggtgc
    16801 gcccatggct tcaatcaggt cgaggatgtt cgggttggcg taggcaacaa cccaggtgga
    16861 gcccatgatg aagatcatgc tgagagtatt cagtttaccc agcgacactt tggttttgtc
    16921 acctttataa ccgaacttca gaatcagacc attcaagcct tccagcgtcc ccagatagtg
    16981 accgaagaaa gatttgaaga tagccacgag tgcgatgatg gaagccgcat attccagtgt
    17041 aatcgcgaac gttgttttcg taccggtcat ggacgcaaag tggttagcca gataagaaag
    17101 cactggaata ttctgcgctt tggcttccgc catgttggcc ggagacagag taaacaggca
    17161 gctaaaggca aagaacatca ccactgcaac catcagcatg ctggcacgag aaatgatttg
    17221 ggaacattta cgttcggtga agtcgcgacc gaagtctttc tcatactctt cacgtttaga
    17281 aaccacgaag gaagagacga ttggcgagaa gttaaaggag aaaaccatga tggaaatccc
    17341 cagccagaca gtgatcagga taccgtcatg accggttaac gacagcgaac cgaggtcaac
    17401 ctggtcgata actgcagagt tccagtaagg gatcagcgac agagaaatca gcaccaggct
    17461 ggcgataaac ggccatacca ggtagctcat cactttaacc atcagatcct taccaaacca
    17521 gatgacgaaa gccatcagca gcaacaggaa cagcgccaca aagccgcgat tcagcggtgc
    17581 aaatccgagc tggttttccc agaacgtcat aaaggtattg gtaatagtaa cgccataaat
    17641 ccacagcagt gggcaaatcg cgaagaagta caggaacgtg ataaccacgc cgccagtttt
    17701 accaaaatgc tcttccaccg tttccgtaat gttgccggaa gggttagagc cagaaagaca
    17761 cagacgcgcc agcgcccggt ggcaataaaa tgcgatgggg tatgccaata ccaacatcag
    17821 aagaatcggg atcagtccgc caaaacctgc gcggataggg aagaacagca ccccggcgcc
    17881 gattgccgta ccaaacaagc ctaacgtcca tgtggtatct gatttacgcc aggacgattg
    17941 ttttgtctgg ctggatacaa tgctatctga agtactcata tcctatcctc aacgaattaa
    18001 ttaagcgtca acgaaaccgg tgatttgaga gacgcgagaa agatcgatat tgccgccgga
    18061 aataatactg acggtttttc tattttgaat atattggtct aatttaccgc ttaataatgc
    18121 agcacatgcc agagcgcctg cgccttcggt gacgacttta ttgcgctgaa ttaaggcaat
    18181 catactgttt ctgatttcgt cttcgctgac cagcacgatg tcatcgacta attcacgaac
    18241 gatttcgtaa gttaaattac ccgggcggga gacatcacaa ccatccgcca gggtgccggt
    18301 agttcggtgc gtggttattt ctccggagtg gaaagaagcc gccatgccgt gaacgttttc
    18361 tgactgtacg ccaataacac gaatggtcgg gttaatagat ttaattgcca ccgcaatacc
    18421 agcaattaaa ccaccaccac caattggcac aatcacgtta tcgacatcat agagatcttc
    18481 cataatttcc agaccaatcg ttccctggcc agcaatcact ttcggatcat cgtaaggtgg
    18541 gataaaaata cgaccttcca tttcgacaat ttcgctcact ttagcgatag tgtcgttgaa
    18601 gttatcacca tgcagaacga cttctgcgga gtagtcgcac gttgccgcta ctttggattt
    18661 tggcgcacct tttggcatca ccactttacc gtcgataccc agcatcgcgc aggagagcga
    18721 aaccccttgc gcatggttgc ccgcagaaca ggccaccacg cctttgcgtt tttccgcatc
    18781 ggtcagtgaa cttaatttat taaatgcgcc acgaatttta aatgaacccg tacgctgcat
    18841 attttcaaac ttcaggaata tttcaccttt gcaacgttca ctaaaatagt tggagcgagg
    18901 catgcctgtt ttataaattc gcccagccag tcgttgtttc gcttcaataa tatcatcaat
    18961 agcaaccggc agatcgtatg taatatgcat tataaaacct cttcgcctga attaaatatg
    19021 taggtaaccg taaccgacac ctgcaaaaca gacaggtgga ttatttatgt ttaaataaaa
    19081 acaaacaact aaccaacttc tattaattgc cttcgtctac acccattata agatgaatac
    19141 tctttggcta attccaccaa aaccgatgct gcttttttaa tacgataatt tttcgaccat
    19201 accgcggcat attgtgccac aggtaatgtt tcttcaaccg gaatagtaat aaattgatta
    19261 gaaccaaaag gtgacgtcat atcacaagga attacagtta agaaatcagc attgagaaca
    19321 agattataaa ttgtcacgac tgagtcggtt ttaacgatgt tttcaatact gatgccattt
    19381 ctttgtaacg tagtaagcag ttcgctgtag taccccatat tagtttgtgg caacacccac
    19441 tgttcgttct tcaacgactc cagcgtggtg gtgccggtgc atgttcggga cttactggct
    19501 accagcacaa actcggactc gaacagcggc tcaacatgta aatcctgaag cttcatttct
    19561 gcacttaacg taccaatcgc aaaatccagt cgaccgtcgc ggattgccgg taagaacgaa
    19621 gacagttgcg cttcatacat agaaacctgc gctttcggga acacctcttt gaacttgttg
    19681 atcatccctg acataaaagt aaaaccaatc aatgaaggaa aaccaaatga gacttccacc
    19741 accgcttcag aagacatacc gcttatctca ttaaccatat ttttcatttc acgggtaatg
    19801 gattcggaac gggagagtaa caattgaccg gcaggtgtta atgttacacc ggtatttttc
    19861 cgcaccacta attccacacc aaaataatct tcaatatcgt taatgatttt actgacggcc
    19921 ggttgagtta accctaattc ttttgcagcc gagccgatag agccacttct aatgacttcc
    19981 tgaaagacta ccaggtgctg cgttttcgga agaagaatag tgctcataac gacctacgtt
    20041 aattacctca ttgacggcat gaagtgtatc aaaatgaaat aaacaggata tgtgcgacca
    20101 ctcacaaatt aactttcatt actttccaag gtatcgttat tacaattaaa catctttaaa
    20161 tatcaacaag ttaaagcata aaaatcagca taaaccctga tttttatcaa aaaaaaatga
    20221 cggggataac catattttat ggcgataaca tcattcgtta tgtggttaat acaaaaaagg
    20281 ggctgagacc atatttcaag caattaccgg ataattacca ggcaaaattt gagttaaatt
    20341 taatgagcaa gttctcaaat tttattataa ataaaccatt ttcagcgata aatactgcgg
    20401 cacgtcacat tttttcacgc tatttgttgg agaacaaaca tttattttat caatatttta
    20461 aaatttcgaa tacaggtatt gatcatctcg aacaattgat taacgtcaac tttttctctt
    20521 ctgacaggac gtcattttgt gaatgcaatc gttttccata aattcttctc ccctcatagg
    20581 cgacgaatag cattttgtgt tgaggatcac aaaacgaata attgctggtc gccgcgataa
    20641 ggtcagacga agtcaacaag ggggattttc actgtgcttt acatcagcgt aggacacaga
    20701 atgttgcttc gtttactgta acgccgggta aatgagcgtt ttttgatagt gcgtaagtgt
    20761 cgtgcgagga aaataagcat ctattattgt ttgtcatgac gacaaggcag gcatcaatga
    20821 aaggcttccc aattgcacat atttttcacc cttcaatccc gccaatgcac gcagtggtta
    20881 acaatcacaa tagaaatatt gattattgga cggtaaaaag aaagtttgca gaaattgtct
    20941 ccaccaatga cgttaataaa atttacagta taagtaatga actccggaga gtattatctg
    21001 caattactgc attgaatttc tatcaaggcg atgttccttc tgtcatgatt ccgaatccaa
    21061 ccggaaaata tgagtccatt catcatagat atttctacag gagaacatga tgattatatc
    21121 atacaaacat tagatgtagg cacttttgca ccttttggtg aacaatgtac ttgctcagcc
    21181 gtcaataaaa aagagctgga atgtattaag gagacgattt ctaagtattg cgcaaagttc
    21241 acccaaaaag aagccatctt aacaccccct aggcacttta ataaaacctg cattacttca
    21301 gattgttggc aaattctctt tttttcaccc gatcatttta ataatgattt ttattgatta
    21361 tttataaagg ataaaataat gttccctgtt tcatcaattg gtaatgatat tagtagtgat
    21421 ttagtccgtc gaaaaatgaa tgaccttcct gaaagcccca taggaaataa cctcgaagca
    21481 ctagcaccag gtatagaaaa actaaaacag acctctattc aaatggtcac tttacttaac
    21541 gcgttacaac caagtggaaa atgcattatc actggtgatt ttcaaaaaga attagcgtac
    21601 ttacaaaatg taattcttta taatgactcg tctcttcgtc tggatttttt aggttataac
    21661 acccaaatta ttcaacgatc ggacaatacc tgcgaactta ccattaatga accgttaaaa
    21721 aactaggaaa tatctacagg caatatcaat gttaattgcc cattaaaaga tatttacaat
    21781 gaaatcagga ggttgaacgt aatctttagt tgtgggactg gagatatagt tgatctatcc
    21841 tctccggact tacgtaatgt cgatttagat tattatgatt tcacagataa acatatggct
    21901 aatactattc taaatcattt taaattggat tcaacaaatt ttactaatgc caacatgttt
    21961 caggttaatt ttgttagttc aacacaaaac gccacaatct cctgggatta tttactaaaa
    22021 ataacgcctg ttttaataag cattagcgat atgtattctg aagaaaaaat caagtttgtc
    22081 gaaagttgtt taaatgagct tggagacatt accgaagaac aattaaaaat tatgagattt
    22141 gcaattataa aatctatacc aagggcaact cttacagata aattagaaaa tgaattaaca
    22201 aaagaaatat ataaaagctc atcgaaaatc aacaattgct tgaacagaat taaattacca
    22261 gagatgaaag aattctcatc aaaaaaaaca tatgattaca tcgatacaat cattgaagat
    22321 tatgaaaata tcaaagaaaa tgcttatctg gtcgtccccc aaattaatta tactatggat
    22381 ttaaacatag aagactctag ctcagaagag ttaataccct cgagaaagac gaaaattctc
    22441 cggacaatgg ctttgaggtc ggggaatata acacatatga agcatataac tcagagaatc
    22501 aatattttac cagagaggac tatacgtata attacgacct tttaaatgca atatagactt
    22561 aataacttct tacaaagtct attattaata ccgtagatat tcatcttacc ctgtgcacca
    22621 aaagcaaaaa cttatcgacg cgggtttgaa actgatggca accgcaaaaa tgcctgatgc
    22681 gctacgctta tcaggcctac gccatctctg caatatattg aatttgcgtg cttttgtagg
    22741 caggataagg cgttcacgca gcatccggca tgaataaagc gcactcaaca atctgaaacc
    22801 cgccggaacg gtttattacg tacatcaggt gaaactgacc gataagccgc tttcttttgg
    22861 gtatagtgtc gtggacagtc attcatcttt ctgcccctcc aaaagcaaaa acccgccgaa
    22921 gcgggttttt acgta
//
LOCUS       DAJTSA010000060        22651 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000060 DAJTSA010000000
VERSION     DAJTSA010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22651
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.060"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..877)
                     /locus_tag="OO369_002519"
                     /pseudo
     CDS             complement(1..877)
                     /locus_tag="OO369_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            885..1385
                     /gene="tnpA"
                     /locus_tag="OO369_002520"
     CDS             885..1385
                     /gene="tnpA"
                     /locus_tag="OO369_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR5982756.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            complement(1424..1771)
                     /gene="grxD"
                     /locus_tag="OO369_002521"
     CDS             complement(1424..1771)
                     /gene="grxD"
                     /locus_tag="OO369_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR5982757.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            2106..2933
                     /gene="mepH"
                     /locus_tag="OO369_002522"
     CDS             2106..2933
                     /gene="mepH"
                     /locus_tag="OO369_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR5982758.1"
                     /translation="MARINRISTTLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            3061..3642
                     /gene="sodB"
                     /locus_tag="OO369_002523"
     CDS             3061..3642
                     /gene="sodB"
                     /locus_tag="OO369_002523"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR5982759.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(3788..4957)
                     /locus_tag="OO369_002524"
     CDS             complement(3788..4957)
                     /locus_tag="OO369_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5982760.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTNISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(5123..5212)
                     /gene="ynhF"
                     /locus_tag="OO369_002525"
     CDS             complement(5123..5212)
                     /gene="ynhF"
                     /locus_tag="OO369_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR5982761.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            5511..6536
                     /gene="purR"
                     /locus_tag="OO369_002526"
     CDS             5511..6536
                     /gene="purR"
                     /locus_tag="OO369_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR5982762.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(6533..7465)
                     /locus_tag="OO369_002527"
     CDS             complement(6533..7465)
                     /locus_tag="OO369_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5982763.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            7578..8789
                     /locus_tag="OO369_002528"
     CDS             7578..8789
                     /locus_tag="OO369_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR5982764.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            9080..10228
                     /gene="cfa"
                     /locus_tag="OO369_002529"
     CDS             9080..10228
                     /gene="cfa"
                     /locus_tag="OO369_002529"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR5982765.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(10268..10909)
                     /gene="ribE"
                     /locus_tag="OO369_002530"
     CDS             complement(10268..10909)
                     /gene="ribE"
                     /locus_tag="OO369_002530"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR5982766.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            11124..12497
                     /gene="mdtK"
                     /locus_tag="OO369_002531"
     CDS             11124..12497
                     /gene="mdtK"
                     /locus_tag="OO369_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR5982767.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(12538..13794)
                     /locus_tag="OO369_002532"
     CDS             complement(12538..13794)
                     /locus_tag="OO369_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR5982768.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            14102..14178
                     /locus_tag="OO369_002533"
     tRNA            14102..14178
                     /locus_tag="OO369_002533"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14136..14138,aa:Val,seq:gac)
     gene            14183..14259
                     /locus_tag="OO369_002534"
     tRNA            14183..14259
                     /locus_tag="OO369_002534"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14217..14219,aa:Val,seq:gac)
     gene            14367..14672
                     /locus_tag="OO369_002535"
     CDS             14367..14672
                     /locus_tag="OO369_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR5982769.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            14798..16220
                     /locus_tag="OO369_002536"
                     /pseudo
     CDS             14798..16220
                     /locus_tag="OO369_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(16232..17044)
                     /locus_tag="OO369_002537"
     CDS             complement(16232..17044)
                     /locus_tag="OO369_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5982770.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(17048..17833)
                     /locus_tag="OO369_002538"
     CDS             complement(17048..17833)
                     /locus_tag="OO369_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR5982771.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(17830..18498)
                     /locus_tag="OO369_002539"
     CDS             complement(17830..18498)
                     /locus_tag="OO369_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR5982772.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(18562..19200)
                     /locus_tag="OO369_002540"
     CDS             complement(18562..19200)
                     /locus_tag="OO369_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR5982773.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(19213..21314)
                     /locus_tag="OO369_002541"
                     /pseudo
     CDS             complement(19213..21314)
                     /locus_tag="OO369_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(21335..21961)
                     /locus_tag="OO369_002542"
     CDS             complement(21335..21961)
                     /locus_tag="OO369_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR5982774.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(22417..22626)
                     /gene="fumD"
                     /locus_tag="OO369_002543"
     CDS             complement(22417..22626)
                     /gene="fumD"
                     /locus_tag="OO369_002543"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR5982775.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         5632 a         5865 c         5270 g         5884 t
ORIGIN      
        1 attttgcccg gacattgcgc cccggctggc tgaccgtacc cgctgctgac tttgacatgt
       61 tgctgacctt caaatcctca atgactatca ttgcgtggtt tttgctgacg gccgttgtga
      121 ctttgtgaag gtagtccctg cggatatttg cgatacagga atgcagtcgc tgtattttgc
      181 gtttctgctt ctgccagttg ttgctgaatt tgaccttgcg gcttaactgt cgctgaagtc
      241 tcgccagcgt cttctggttt ttctgaaaac tgtttacagg ctcaaagact gtgccatctg
      301 acagcgtggc gagtttagcc acgccagcat ccagcccgac cattgatgct gaagggtgag
      361 ccggagtgga tacttcactt tctgtctgaa tactgatgta ccacgtaccg caggactggc
      421 tgacagtgac atttttcaca acacccgtga cctgacggct attccggtag cgcatccagc
      481 ccagtttcgg cagaaaaata cggctgtttt cctgatcgag cttaacgccc tgcgggtagc
      541 ggaatgcatc attctgtcac cgctttttga atcggggaaa agcagcccgc ttccggaaga
      601 agtttttgta agcccgctca aggtctttca gtgactgttg caatggctgt gagggagaat
      661 ctttaagcca ttgcgtttca gtggcatttt tccactcaac cagccaggaa gccattttcc
      721 cgtaagggat gtatttatta ccggcctcat gattctcatt ctgacgtgcc agagcacgat
      781 tgaaaacgaa acgacatgcg cctgcgaagc gcctcatttc acactcctgt tgaccacctg
      841 gtcttaactg gaatttaaat gcctgtaatc gtttcacagg tcaaatgata ctttggtctg
      901 tgaaaaaaga aaccgatatc cggcgtggaa ggcattgtgt tttcctgatg catgttcacc
      961 tggtctttgt caccaaatac cgacgccaga tttttgacca tgacacgaca gaaaaactac
     1021 gcacttactt ttcaaatgta tgtgctgatt ttgcagctga actggttgaa atggatggcg
     1081 aaccagatca cgtccatttg ttaattaact atcctcccaa actggcgata tccagtctgg
     1141 taaacagcct caaaggcata tcgaataggt tactgcgacg agatcgacca gatattgcag
     1201 tcaggtatta ctacaaaggc gttttgtgga gtcctggcta ttttgccagt agctgcgaag
     1261 gtgcgccaat atccgtcatc cgccagtaca ttgaacaaca gcaaacacct ggtcaggtgg
     1321 aaaaccgcgc cttatatccc cgccctgaag gacgaggttt tacggcgcac cggataaagg
     1381 actgacggat gcccgttcgc atccgtcagt attgcaggac ggattattcc gcgtccggct
     1441 cttcagactt gtatttagcg gcagtttctt tgatcagctg ctgcagttcg ccacgctgat
     1501 acatttcgat cacgatatca caaccgccga ccagctcgcc gtcaacccac agttgcggga
     1561 aggtcggcca gttagcatat ttcggcagtt ccgcacgaat gtccggattc tgcagaatat
     1621 caacataggc aaaacgttcg ccacatgcgg caagcgcctg gactgcctgg gcagagaaac
     1681 cgcagctcgg cagtttcggt gaacctttca tgtacagcag gatcgggttt tcagcaatct
     1741 ggcgttggat tttttcgata gtggtgctca ttgtcttgct tcctcaaact tctttacggc
     1801 agtaatctga cattgtagcg ggtcagtgcg gcatcggaaa ataacatttt catcacgctt
     1861 ttgctatttt atccctttgc tctatttgtt gcatttcaaa tattcgtttc ttctctgatg
     1921 cgcggcaaac tggcgtattt ataaccattt ttagctttca ctgctgcatt tttttgcgct
     1981 cgccaacgaa acgtattttt taacaataaa agctattaac tttctcttct tctatgcatt
     2041 agaatcatca agttttgtaa atcagacgca ggcatgatag acctgcctta acagagggac
     2101 gctcagtggc gcggataaac cgtatttcga ccacgctctg tgctttactt tttaccaccc
     2161 tgcctttaac gcctatggcc catgcttcaa agcaagccag ggagagttct gctaccactc
     2221 atatcaccaa aaaagcagat aaaaagaaaa gcacggcaac caccaaaaag acccagaaag
     2281 ccagccagaa aacagcgaaa aaagccgcca gtaaaagtat gaccaaaagc aaaaccgctt
     2341 cttctgttaa aaaatcttcc attaccgctt ctaaaaacgc caaaactcgc agcaaacacg
     2401 ccgtcaataa aacggcctca gccagcttta ccgaaaagtg taccaaacgt aagggctata
     2461 aatcgcattg tgtgaaagtc aaaaatgccg cgtcaggaac ccttgccgac gcgcacaaag
     2521 cgaaggtgca aaaagccacg aaagtggcaa tgaataaact gatgcagcaa attggtaagc
     2581 catatcgttg gggcggcagc tcaccgcgta ccggttttga ttgcagcggc ctggtttatt
     2641 acgcttataa agatttggtg aaaattcgta ttccgcgtac ggcgaatgaa atgtatcacc
     2701 tgcgtgatgc aggacctatc gaacgtagtg agctgaaaaa cggcgacctg gtctttttcc
     2761 gtactcaggg acgcggtaca gccgatcatg tcggcgtgta tgtcggcaac ggcaaattta
     2821 ttcagtcacc gcgcacaggt caggaaattc aaatcacttc tctcagtgaa gactactggc
     2881 agcgccacta tgttggcgct cgtcgggtaa tgaccccaaa aacacttcgc taaaacttta
     2941 ccctgttgtt acggcaacag ggtaagttca tcttttgtct caccttttaa tttgctaccc
     3001 tatccatacg cacaataagg ctattgtacg tatgcaaatt aataataaag gagagtagca
     3061 atgtcattcg aattacctgc actaccatat gctaaagatg ctctggcacc gcacatttct
     3121 gcggaaacta tcgagtatca ctacggcaag catcatcaga cttatgtcac taacctgaac
     3181 aacctgatta aaggtaccgc gtttgaaggt aaatcactgg aagagattat tcgcagctct
     3241 gaaggtggcg tattcaacaa cgcagctcag gtctggaacc atactttcta ctggaactgc
     3301 ctggcaccga acgccggtgg cgaaccgact ggaaaagtcg ctgaagctat cgccgcatct
     3361 tttggcagct ttgccgattt caaagcgcag tttactgatg cagcgatcaa aaactttggt
     3421 tctggctgga cctggctggt gaaaaacagc gatggcaaac tggctatcgt ttcaacctct
     3481 aacgcgggta ctccgctgac caccgatgcg actccgctgc tgaccgttga tgtctgggaa
     3541 cacgcttatt acatcgacta tcgcaatgca cgtcctggct atctggagca cttctgggcg
     3601 ctggtgaact gggaattcgt agcgaaaaat ctcgctgcat aataactgat ggcaaatgca
     3661 gcattgcctg atgcgcttcg cttaccatgc caacgcggat gaggcgttaa cagcgcattc
     3721 ggcaagataa atcgcacgtt gtcagcaact gtaacgcaga aggttatcct tctgcgtttt
     3781 tgtttaatta gctgttagca acgcaaactg tttcaggttg ttttctggct gacataaaca
     3841 ccagcaataa tgccagtccc gcgacaatcg cccccgtcac cggcacaaag ctgtatccca
     3901 gcccagcgga aattaccgca ccaccagcag ctgctcccag cgcatttcca agattaaagg
     3961 caccaatatt gactgatgaa gacagacctg gcgcttcact ggcgacatgc atcacgcgca
     4021 tctgtaacgg aggtacaacc gcaaaggttg ctgcgcccca caccaccatg ctaatagctg
     4081 cgccgaactc attgcgggcc aggaacggga ttgccagcat aatcaccatc agcagcaaca
     4141 aaaagccttt caacgtgccg ttaactgaac gatctgccag tttgccgccg agatagttac
     4201 cgatagagaa tccgacacca atcagcacca gcattgccgt gacgaacacc ggtgttgcgt
     4261 gggtaatact ttgcagtacc ggagagatat tggtgtagag ggtaaacatt gcaccagctc
     4321 ccagtaccgt cgtcagcaat gcagacagca cctgcggacg cattaatacc gccagctctt
     4381 ttttcacttc aggtcgtgcc cctgtaccac ctttaggtaa tgagaagaac agacttacca
     4441 ttgaaatcac tcccagcccc gccgttgcca gaaatgacat ccgccagccg atggtttcac
     4501 ccaaccaggt cgccgccggc acgccaccga tatttgccag ggttaacccc ataaacatag
     4561 tggcaactgc gctggcctgt ttatgttttg gcaccacgct tgcggccacg actgaaccca
     4621 aaccaaaaaa tgctccgtga ttcaggctgg tcaaaatacg tgaaagcatc agggtcatat
     4681 aatccggcgc gatggcggaa agaacgttgc ctagcgtgaa aattgccatc aggaaaatca
     4741 acgcactgcg gcgggcacga tgagaaagta gaagcgtcat cagcggcgcg cccaccatta
     4801 cgccaactgc ataggcactg attaacattc cggcagcggg aatcgagaca tccacaccgc
     4861 gcgcaatgac gggcaacaag cccattggcg agaactccgt tgtcccgata ccaaacgcgc
     4921 caatcgccag cgccagcaac ggatagttaa ttttcatgcc ttatctccac ctcttcgcgt
     4981 cattacgcga tattcattaa agtggcgaaa gcatgacagc aatcacaaaa aaatgaaaat
     5041 aacaaaaaga gaaaacactt ttgccatttt gctaacaaac aggaaggaga tgcgagggag
     5101 aacgcgctcc ctcgagagga aatcagtgca gcgcggcagt caaacccacg gctacgatca
     5161 aaccgaggac gataaccgtt gttaccagtg aaaatttaag gtcggtgctc atcaagtttt
     5221 ctcctttttt attaccacac aaaaagtgat attacgcatt tttacacact gtgatgaaaa
     5281 aatctcccgt catttataat gataagtgtt tttaccactt ccccttttcg tcaagatcgg
     5341 ccaaaattcc acgcttacac tatttgcgta ctggccattg accccttcct gacgctccgt
     5401 gtcgtttttc cggcgtaccg caacactttt gttgtgcgta aggtgtgtaa aggcaaacgt
     5461 ttaccttgcg attttgcagg agctgaagtt agggtctgga gtgaaatgga atggcaacaa
     5521 taaaagatgt agcgaaacga gcaaacgttt ccactacaac tgtgtcacac gtgatcaaca
     5581 aaacacgttt cgtcgctgaa gaaacgcgca acgccgtgtg ggcagcgatt aaagaattac
     5641 actactcccc tagcgcggtg gcgcgtagcc tgaaggttaa ccacaccaag tctatcggtt
     5701 tgctggcgac cagcagcgaa gcggcctatt ttgccgagat cattgaagca gttgaaaaaa
     5761 attgcttcca gaaaggttac accctgattc tgggtaatgc gtggaacaat cttgagaaac
     5821 agcgggctta tctgtcgatg atggcgcaaa aacgcgtcga tggtctgctg gtgatgtgtt
     5881 ctgagtaccc agagccgttg ctggcgatgc tggaagagta tcgccatatc ccaatggtgg
     5941 tgatggactg gggtgaagca aaagctgact tcaccgatgc ggtcattgat aacgcgttcg
     6001 aaggcggcta catggccggg cgttatctga ttgaacgcgg tcaccgcgaa atcggcgtca
     6061 tccccggccc gctggaacgt aacaccggcg caggccgcct tgccggtttt atgaaggcga
     6121 tggaagaagc gatgatcaag gtgccggaaa gctggattgt gcagggtgac tttgaacctg
     6181 aatccggtta tcgcgccatg cagcaaatcc tgtcgcagcc gcatcgccct actgccgtct
     6241 tctgtggtgg cgatatcatg gcaatgggcg cactttgtgc tgctgatgaa atgggcctgc
     6301 gcgtcccgca ggatgtttcg ctgatcggtt atgataacgt gcgcaacgcg cgctatttta
     6361 cgccggcgct gaccacgatc catcagccaa aagattcgct gggtgaaaca gcgttcaaca
     6421 tgctgttgga tcgtatcgtc aacaaacgtg aagaaccgca gtccattgaa gtgcatccgc
     6481 gtttgattga acgccgctcc gtggctgacg gcccgttccg cgactatcgt cgttaatcac
     6541 ccgttgcggg agtctcttcc ggctcccgca gccactcctt attcagcgtc tcactatcgc
     6601 tgagatactc aagcagccag gttaacgcag gcgacatatc attttgctgc catgtcagac
     6661 aacatgccga atccgggaag gggttttcca gttcaagtgc tacccacttc ccctcattaa
     6721 gccacggttt ggcgaaatgt gttggcacca tccctatgca taatcctgcc gagatacagg
     6781 ttgccgatga ttcccagtca ggtacgacaa gtcttttttg gttatccagc aaccaggtaa
     6841 tacgtttagg tagcgttcgc gaggtgtctt cgcgcaccaa cgacggccag ttgcgcaacg
     6901 tatcatcgct gaacgggcca tccatcaacg ccagcgggtg gtgactggca acaacgcaac
     6961 tccagcttag catccccata tcccggaagg cataacgacc gcctaccgga atcgcgcgtg
     7021 ttgcgccaat tgccagttcc acgcgcccgt cggaaagcgc atcccagaca ccgttgaaca
     7081 cttcctgaaa gacaagaagt tcgacatcat caaaatggcg ataaaaatca acgatcatct
     7141 gccgtgtacg ttctggcctg acaatattat ccactgcgat agctaactga ccgcgccagc
     7201 cgttcgctat ctgctgacat tgctggcggg tgatctgcat ttttttgaca acagagcgcc
     7261 cttctttgag aaaccacgct ccagcagcgg tcagctccac atcacggtgc cgtcgttcaa
     7321 agagcggtac cgccagccac tcttccagct gacgcacggt atagctgacc gcagaaggaa
     7381 cacgatgcag ctcctgtgcc gcagcgctaa aactaccatt acgcgctacc gcatcaacaa
     7441 cttcgagtga atattctgac cacatagtct gcctgcaaaa tttttgaaac cagtcatcaa
     7501 atattaccgt ttcacaacac taatttcact ccctacactt tgcggcggtg tttaattgag
     7561 agatttagag aatatacatg caacctggga aaagattttt agtctggctg gcgggtttga
     7621 gcgtactcgg ttttctggca accgatatgt atctgcctgc tttcgccgcc atacaggccg
     7681 acctgcaaac gcctgcgtct gctgtcagtg ccagccttag tctgttcctt gccggttttg
     7741 ccgcagccca gcttctgtgg gggccactct ccgaccgtta tggtcgtaaa ccggtgttat
     7801 taatcggcct gacaattttt gcgttaggta gtctggggat gctgtgggta gaaaacgccg
     7861 ccacgctgct ggtattgcgt tttgtacagg ctgtgggggt ctgcgccgcg gcggttatct
     7921 ggcaagcatt agtgacagat tattatcctt cacagaaagt caaccgtatt tttgcgacca
     7981 tcatgccgct ggtgggtctg tctccggcac tggctcccct gttaggaagc tggctgctgg
     8041 tccatttttc ctggcaggcg attttcgcca ccctgtttgc cattaccgtg gtgctgattc
     8101 tgcctatttt ctggctcaaa cccacgacga aggcccgtaa caatagtcag gatggtctga
     8161 cctttaccga cctgctacgt tctaaaacct atcgcggcaa cgtgctgata tacgcagcct
     8221 gttcagccag tttttttgca tggctgaccg gttcaccgtt catccttagt gaaatgggct
     8281 acagcccggc agttattggt ttaagttatg tcccgcaaac tatcgcgttt ctgattggtg
     8341 gttatggctg tcgcgccgca ctgcagaaat ggcaaggcaa gcagttatta ccgtggttgc
     8401 tggtgctgtt tgctgtcagc gtcattgcga cctgggctgc gggcttcatt agccatgtgt
     8461 cgctggtcga aatcctgatc ccattctgtg tgatggcgat tgccaatggc gcgatctacc
     8521 ctattgttgt cgcccaggcg ctgcgtccct tcccacatgc aaccggtcgc gccgcagcgt
     8581 tgcagaacac tctgcaactg ggtctgtgct tcctcgcaag tctggtagtt tcctggctta
     8641 ttagtatcag cacgccattg ctcaccacca ccagcgtgat gttatcaaca gtagtgctgg
     8701 tcgcgctggg ttacatgatg caacgttgtg aagaagttga ctgcccgaat catggcaatg
     8761 ccgaagtcgc tcatagcgaa tcacactgat ctatatcgat atacttatac ttaggctgct
     8821 aacaaaattt tgttgtatga ttgaaattag cggcctatac taatttcgag ttgttaaagc
     8881 tacgataaat attatgtttt tacggggaca ggatcgttcc cgactcacta tggatggtca
     8941 tttcggcaag ggttcctcct ttccctctgt tctacgtcgg attatagact cgcggttttt
     9001 tctgcgagat ttctcacaaa gcccaaaaag cgtctacgct gttttaaggt tctgatcacc
     9061 gaccagtgat ggagaaacta tgagttcatc gtgtatagaa gaagtcagtg taccggatga
     9121 caactggtac cgtatcgcca acgaattact tagccgtgcc ggtatagcca ttaacggttc
     9181 tgccccggcg gatattcgtg tgaaaaaccc cgattttttt aaacgcgttc tgcaggaagg
     9241 ctctttgggg ttaggcgaaa gttatatgga tggctggtgg gaatgtgacc gactggatat
     9301 gttttttagc aaagtcttac gcgcaggtct cgagaaccaa ctcccccatc atttcaaaga
     9361 cacgctgcgt attgccggcg ctcgtctctt caatctgcag agtaaaaaac gtgcctggat
     9421 agtcggcaag gagcattacg atttgggtaa tgacttgttc agccgcatgc ttgatccctt
     9481 catgcaatat tcctgcgctt actggaaaga tgccgataat ctggaatctg cccagcaggc
     9541 gaagctcaaa atgatttgtg aaaaattgca gttaaaacca gggatgcgcg tactggatat
     9601 tggctgcggc tggggcggac tagcgcacta catggcatct aactatgacg taagcgtggt
     9661 cggcgtcacc atttctgccg aacagcaaaa aatggctcag gaacgctgta aaggcctgga
     9721 tgtcaccatt ttgctgcaag attatcgtga cctgaacgac cagtttgatc gtattgtttc
     9781 tgtggggatg ttcgagcacg tcggaccgaa aaattacgat acctattttg cggtggtgga
     9841 tcgtaatttg aaaccggaag gtatattcct gctccatact atcggttcga aaaaaaccga
     9901 tctgaatgtt gatccctgga ttaataaata tatttttccg aacggttgcc tgccctctgt
     9961 acgccagatt gctcaatcca gcgaacccca ctttgtgatg gaagactggc ataacttcgg
    10021 tgctgattac gatactacgt tgatggcgtg gtatgaacga ttcctcgccg catggccaga
    10081 aattgcggat aactacagtg aacgctttaa acggatgttt acctattatc tgaatgcctg
    10141 cgcaggtgct ttccgcgccc gtgatattca gctctggcag gtggtgttct cacgcggtgt
    10201 tgaaaacggc cttcgagtcg ctcgctaaag gctattctat cgccccctct tcgggggcga
    10261 gttcagatca ggcttctgta cctggttgat tcatggcatt ttctcgtgcc gccagcacac
    10321 gttctaccgt atctaccact gcctgagttt gtggatcgat ttcaatgttg acgcgtgcgc
    10381 caagtttttt cttcccaaga gtcgtgcgtt ccagtgtttc cggaattaaa tggacgcaaa
    10441 aacgcgttgg cgtgacttcg ccgacggtca ggctaatacc gtcgatgcca ataaatcctt
    10501 tgtacaggat atatttcatc aactgactat cctggacttt aaaccagatc tggcgattat
    10561 tttccgaggt taatattttc gccacttcag cggtggtcat aatatgacct gacattaagt
    10621 gtccgccaat ttcatcactg aatttcgccg cacgctcaac gtttacccaa tcccccactt
    10681 ttaaatcgcc aagattggta atgcgtaacg tttctttcat caggtcaaaa ctgacatggt
    10741 tgccgttaat ttccgtcacg gtcaggcagc aaccgttatg cgccacggaa gcaccggttt
    10801 ccaggccgtc cagcatgtgg tcgggtaact ccaccacatg cgtacgaaaa tttggtttct
    10861 cgtcaatcga caccagtttt gcggtgccct gtacaatccc cgtaaacata cttacaactc
    10921 ctgaaatcag ttaagacatt ctgttcagca caatagcagg tggaaaacgc ccttaccagt
    10981 gaaggggtaa gaatggctat tttttcactg gagaattaat aaatcctcgc tacaatagac
    11041 tgaatttccc ctgcttcttc tttttgctgc ccattcaggc ggctttttag tctctcatat
    11101 aactacaaat aaaaggtgtt cacgtgcaga agtatatcag tgaagcgcgt ctgttattag
    11161 cattagcaat cccggtgatt ctcgcgcaaa tcgcccaaac tgcgatgggt tttgtcgata
    11221 ccgtgatggc gggcggctat agtgccaccg acatggcggc ggtcgctatc ggtacttcta
    11281 tctggcttcc ggcgatcctc tttggtcacg gactgctgct ggcattaacg ccggttatcg
    11341 cgcaattaaa tggttccggt cgacgtgagc gcattgcgca tcaggtgcga caaggtttct
    11401 ggctggcagg ttttgtttcc gttctcatta tgctggtgct gtggaatgca ggttacatta
    11461 tccgctacat ggaaaacatc gatccggctc tggcggacaa agccgtgggt tatctgcgtg
    11521 cgttgttgtg gggcgcgccg ggatatctgt tcttccaggt tgcccgtaac caatgtgaag
    11581 gtctggcaaa aaccaagccg ggtatggtaa tgggctttat cggcctgctg gtgaacatcc
    11641 cggtgaacta tatctttatt tatggtcatt tcggtatgcc tgagctcggt ggcgttggtt
    11701 gtggcgtggc tactgcggcg gtgtattggg tcatgttcct tgccatggtt tcttacatta
    11761 aacgcgcccg ctccatgcgc gatattcgta acgaaaaagg caccgctaag cccgagcctg
    11821 cggttatgaa acgactgatt caactcggtt tgccgattgc gctggcactg ttctttgaag
    11881 tgacactgtt tgccgtcgtg gctctgttag tgtctccgct cggtattgtt gatgtcgcag
    11941 gacaccagat tgccctgaac tttagttcac tgatgtttgt gcttccaatg tcgctggcgg
    12001 cagcggtaac tatccgcgta ggttatcgtc tgggtcaggg ctcaacgctg gatgcgcaaa
    12061 ccgctgcgcg gaccgggctt atggtgggtg tctgtatggc aaccctgacg gccattttca
    12121 cggtttcact gcgggagcaa atcgccctgt tgtacaacga caatcccgag gttgtaacgc
    12181 tggctgcgca tttgatgttg ctggcggcgg tatatcagat ttctgactca atccaggtga
    12241 ttggcagtgg gattttgcgt ggttataaag atacgcgttc cattttctat atcaccttta
    12301 cagcttactg ggtgctgggc ttgccaagcg gctatattct ggcactgacc gatctggtcg
    12361 ttgaacctat ggggccagca ggcttctgga taggctttat tatcggcctg acatcggcag
    12421 ccattatgat gatgctgcgt atgcggttcc tgcaacgtat gccgtcagcc atcattctgc
    12481 aacgagcgtc ccgctaataa agacaaggcg caaccttcac gggttgcgcc tgtgttttta
    12541 cgcaggctgg agcgttgcgc caatcccgtc ttcgtctggc tgtaatttca gagcgttaca
    12601 cagagtttgc cgactggcat ctgttatctc aacgcgtata tccccgttaa cttccccttt
    12661 cacattgatg aagtcaggtg aaatttcttt cgcactggtt tgcaggcaaa tcgctccctc
    12721 tccgcgcagg cgtttcactt tcagttgcac atgcgttgcc gcaccagtga tcagccacag
    12781 ttttccgcca ttcagattga tgttaccaac aatgaaagtg acgctgtcac cttttaccgt
    12841 actgccgtcg agaatcaaca caccgccctg ctgtacagta atcgttgatt gttgcagctc
    12901 tgcgccataa ccaataatca ttgaagcgcc gtccagtaat gcaacactcc cggtcagttc
    12961 aaccggtgcg cgatcttcct ctacgacaaa attttcgtcc gcgctggtgg gtgacaacag
    13021 gatcactgtt ccaccttgta ttaccgcatt ttccagcgta ccggcatgca cttcagcccg
    13081 cccccccaca ttcaccgaca ggttttgcgt cttaccggaa ctgtagagct gaaggccatc
    13141 cgtccccaga cgataaatgg atccattctc aacaatagtg tcagagtctt caccaccgag
    13201 attattcatg gtgccaccag caagcaacgt atcactggct ttcccggcca gcataaaaac
    13261 gccgccctga ttaatctcac aattgctggc ttctccactt ggatacacca acaattcacc
    13321 accttcatct accacgaccg ctgaggttgt cccattaacc aacaggccgc cctcttgatc
    13381 gagaatgatt ttttccgcgc gatgtccttc cagtacacgc aggttaccgc cattttccag
    13441 caacaaaccg caggcataac cctgatcaac gctgaattca ccttcgggat ggcggccatt
    13501 aaccgtagcg agcgtagaga gactaattgc gcctccctcg gactgaacaa tatctgtggc
    13561 aatggcaccg tcgctgaccg atagcgtgcc attgttaccg tactgtgtgc atttggcgat
    13621 cccaccggtt gccactttca accagccagc ccccgtgacc acggtatttt cggctcttcc
    13681 gccagcttca acaatcaact cgccttcagt aagttgcgtc gcgccaagga cctcgccggt
    13741 cttaacaatt tgcacattcc cgtcgctcat caagtttttc gcatcagatc ccatgattta
    13801 ttcctttgct gcatctgtgt gcctttattg ctacctaagt gtaaaggcta cggaggattt
    13861 atccacgaca gatttgagat ggtggcaaac aactctgttt aaactctgat acacgaatta
    13921 ttgggttgta tcagatgtaa atgcgatcct gaataaaaat cgcccttgca aatcaacaaa
    13981 atatagccaa tttgcttaaa agctctgcaa acggtcgaaa tagcgcagaa aattacgttt
    14041 tgcctcttgc cacctttcca ctctgccgct aatattcgtc cccgttgtca cctacaacgt
    14101 tgcgttcata gctcagttgg ttagagcacc accttgacat ggtgggggtc gttggttcga
    14161 gtccaattga acgcaccatc ctgcgtccgt agctcagttg gttagagcac caccttgaca
    14221 tggtgggggt cggtggttcg agtccactcg gacgcaccag attttcttaa tctggtcttt
    14281 tcctttttcc ctctgtttct tctctgtatc caatacgtta aaagatttac actgtcttcg
    14341 tatgcgttat cagaaggaga atcgctatgg caactttgtt acaacttcat tttgctttta
    14401 atggcccatt tggcgacgca atggctgagc agcttaaacc acttgctgag tcgattaatc
    14461 aggaacctgg ttttctgtgg aaggtatgga cagaaagtga aaagaaccac gaagccggtg
    14521 ggatctacct tttcactgat gaaaaaagcg cccttgccta tctggaaaaa catactgccc
    14581 gactgaaaaa tctcggcgtt gaggaagttg tcgccaaagt tttcgatgtc aatgagccac
    14641 ttagtcaaat caatcaggca aaactcgcct gacagaattt aatcaagggc ggttagcgcc
    14701 cttttcatcc ctgtctgaaa tttctcaaat tctaaaaatc tcaaccaaac ttatctgata
    14761 acactaaatt cgaaagaatg cgtacaggta agtaacaatg aaaaaaattg ctattgtggg
    14821 tgccgggcct acggggatct acaccttatt ctcgcttcta cagcaacaaa ctccactttc
    14881 tatttctatc ttcgagcagg ctgacgaggc cggtgtcggg atgccataca gtgatgagga
    14941 aaactcaaaa atgatgctgg caaatattgc cagtattgaa ataccgccga ttaattgtac
    15001 gtatctcgaa tggctacaaa agcaagaagc cagccatctc cagcgttatg gcgttaaaaa
    15061 agaaaccttg cacgatcgtc agtttttacc gcgaattctg ctgggcgaat atttccgcga
    15121 tcaattttta cgattagtag accaggcacg acagcaaaaa tttgcagtgg ctgtttatga
    15181 atcatgccag gttaccgatc tgcaaattac aaatgctggc gtcatgctcg ctacaaatca
    15241 ggatttaccc agcgagacgt ttgatttagc ggtgattgcc acgggtcacg tctggcctga
    15301 tgaagaagaa gcaaccagaa cgtattttcc cagcccgtgg tcaggcctga tggaagcaaa
    15361 ggtcgatgcg tgtaacgtgg gtattatggg aacatccttg agcggactgg atgcggcaat
    15421 ggcagtggct attcagcatg gttcgttcat tgaagatgat aaacaacacg tcgtttttca
    15481 ccgcgataac gcaagtgaaa agctaaatat tacgttgatg tcgcgcacgg gtattttacc
    15541 cgaagccgat ttctattgcc ctattcccta cgagccctta cacatcgtca ccgatcaggc
    15601 attaaatgct gagattcaaa aaggtgaaga gggccttttg gatcgggtat ttagattgat
    15661 agtagaggaa atcaagtttg ctgatccaga ctggagccaa cgcatagcct tagagagcct
    15721 gaatgtcgat tcctttgctc aagcctggtt tgccgagcgc aaacaacgcg acccatttga
    15781 ctgggcagaa aaaaatctcc aggaagtcga acgcaataaa cgagaaaaac atactgttcc
    15841 ctggcgttat gtcattctgc gcctgcatga agccgtacag gaaattgttc ctcatctgaa
    15901 tgaacacgac cataaacggt tcagtaaagg ccttgccagg gttttcatcg ataattatgc
    15961 ggcaatccct tcagagtcta ttcgtcgact actggcctta cgtgaagcgg ggatctgaaa
    16021 tccctgatgt tggcgaagat tatacgttac agcaacccga agatattcgt ggacgcgtag
    16081 cgttcggcgc gttgccctgg ttgatgcacg accagccttt cgttcaggga cttacggcat
    16141 gtgcagaaat tggtgaggcg atggctcggg cggtcgtaaa gcctgcatcc cgtgctcgtc
    16201 ggcgtctttc gtttgattaa aggtaaagct atcagcactc aacaaactga tgctgttcat
    16261 taagacgata gcggactccg gttcgaatat tgttttcacc ttcaatggcg acggcgaagc
    16321 gaactctttc gccgtcatga tatgccagtg cggcgcttcc ccctagcccg agaataattg
    16381 agtcgaccac gcccgtgctc actaccgttg aattttcacc gctggcaatg atgtgtacat
    16441 tgtcgccgct gttggcaatt ctggcgtttg cgccaaagct ggcgatttgc accaggtcac
    16501 cattactggc gacatgacac cgttcaccca atgtacaaac gcgtactcgc attccggtat
    16561 tcgcaatccg actactatct ccagcgctgc ttatccgcga agaattaccg gttacagcaa
    16621 cccgtgcgcg ttcaccggag ctgccaatgt gactgttaaa gccgacactt ccaatccgca
    16681 cgctataacc catactggca atttgcgcgg catacccagc ggttgcgatt cgggcgttat
    16741 cctcacaaca gccgatgcga gcatgatcgt cgctgcgggc aaactgactg caaaatggta
    16801 tctctccggg gtctgtggcc agtttttgca tagaggaaac ttcctgatgc gcgaagtttt
    16861 catccgccag ccattttttc cacgcatact caaccagact ctccgcccag tccgtatatc
    16921 cttcctcaat cagtgcatga cgaatatcgg cataactacc gccgcgtggg aaatggcgca
    16981 ggaaccagcg atacatcacc gctccgatac gccattctcg caggtctgct cgtgtaatga
    17041 tcattcgtta gtgccggtga tagccatcga ccatgctttt aaaggtttcg tgtggcgtac
    17101 gccccgtggt gcaaagataa agatgaccga agataaaaaa gaggcttata aatgccagag
    17161 caaaatgcgc ctgcaataac cagtatctta cgccaggaaa cacatctccc acggcttgcg
    17221 gatagagaca caacagcccc gtcaatagta acaacggcag caatccatac atgacaccaa
    17281 cataggcgac ctgctgtaag ggattaaatt tagactgtgt tgttgccggg aaaggatgtt
    17341 cttccccctg cataatgcca aacaaataaa atcgcgtttg ttttgccgct cgttccagcc
    17401 acccctgacg acgaatgcga tagtggtgac cattatcccc aacggcattg atcagcacaa
    17461 agccgagcca gcatgccagt aacaaaaatc cgcaaacttc atgcaccgca accagacttt
    17521 taaccgcagt tgcgcccacc atcgcaaagt gatttatcag cccacttgcc agcaacaata
    17581 caaagagtaa cgcattcgac cagtgccata atctgaccgc tttgctgtaa agataaacct
    17641 tctcaccatg atcggtggct gatttcttca cgccacgagc acgaagcaag gcgtgcagcc
    17701 ccagcaccaa ccacatccca accagcaata ctccggcaat gatcaaccac accggccaaa
    17761 attcgggagt gaactgtggc acatagttcg ctaactggct ctgaaactgt tcagcatgtt
    17821 gcgacgggtt catacatttt cctttttaat caaatgttga ccgaaccggc gatacagatg
    17881 cggttttccg gcaccaggta gctgatattg atagtattta ttatcctgca accacgcctg
    17941 aatttctggg ctatcttcgc gaccaaagat caatgcgtgt tccggacagg cgctgacgca
    18001 aatgggcgga aagccttttg ccagtcggga ctcagcgcag aaatcacatt tatctgccac
    18061 tttagtgaca gggttaaggt agcgaaccag gtacggacaa gcgccaatgc aataactgca
    18121 gccgatgcac tgcgattttt ctacccgcac gatcccctgc tcatcacgcc acgatgcccc
    18181 cgtcgggcat acgtcgatgc acggagcatc ttcgcagtgc tgacatgact ggcgaaaaaa
    18241 gtgatactgc gtttcgttgt cgttatcggt gactggaatg tgtgctatcg ataaacggct
    18301 tccctgagcg gggacgtggt tagttttacg gcatgcgcgg gcgcaaatat tacagccatt
    18361 gcaccgtgac tcgtcatgaa tcatggcata ccgaacttcc ttttcttgcc tcgtgttcgc
    18421 taacagagaa gacgcagaac cagtaaaaaa gattactgtt cccatcccca gaacaaattt
    18481 gcgtcgagtg aaggacatct gattatcccc taatgcagtg gctgcaactg tccaccctat
    18541 ccatgacatc tccctgaaat ttcaggggtt ctgaaactgc tctacctcga accattcgca
    18601 taagccataa gccttgccat aggtcataaa tcgctcgcta aacaagtaca acaccgccgt
    18661 cgatgactcc acctgccgta tatcggcata ttcaggtgca acatccatcg cggcgatcgc
    18721 ggcttcaatc tgcgcttcct ggaagtaata tggcgcttgc atcagcatgg cgactttgta
    18781 aggacgagga tacgtctggc actcaaaacg aacggcgtgt gcaatggcgc gacaaatgtc
    18841 ctgctccaca acttgcaaag acatcgccgc gtagttttca ctcatggcct gcgttgagta
    18901 atagtaatca tcctgacttc ccgtcataca agcgatatcc gcgtagtctc cgttctgctt
    18961 aatgctttcc agcaaaactg acagttcctc ttctgcaacc gaatagggtg gcgagagaaa
    19021 cacggtgcgc gccaccagct gcccagccgc agaatgctgg cgaatgaact cagccaggat
    19081 ttcagcaggt tctggttcgg ttaccgtatc aacggggtga cgcatcccct gcaaccattg
    19141 ccgcttcgcc tcgtcaactt cgctgacttt cgctaacggc aattcatcct gatggttcat
    19201 cttacccatt ccttacgcag gcagtagatt gtgtgctgcc agatcggcgg caatatcttc
    19261 cagccccaga cgctgcaatg tttcgcgggt tggacaacca agctgtgggt cccagcccat
    19321 ttctttgtag aacatcgtga gcgaagcatg catatcgtca cgatccattt tgtcagtacc
    19381 ttcagtaaag accgggatct gcggatcctt gtcgaaaacc caggaacaga taagatcgtg
    19441 ttcattacgc atatctttgg tttgcatcgg ttttaccgtg taggcacgat gcaacgtaaa
    19501 gatacgctct gcagctaaat ctaatttttc ctgagtcatc tcttcgccgg tgatcgcttt
    19561 gaagaatttg gcttcaagcg ccagatcgcc ccggtaatta cggcttttca gcggcgaaac
    19621 ggtcattggc cagacccagt tgcacagagt gacggcgtta tgcaaacaga cccgcaacag
    19681 cgaccacttt gcatatttaa tttttgcgtc attgattggc gtgtagtttt tggtttcatc
    19741 gtaagcatct tcagaaccaa aaagttcttt cgccacttca cgttgcagtt tcaatggcaa
    19801 gccggaacca ataaagttga tatgggtatg cgtcatgcag tcacggttga acatacactt
    19861 aacaatggaa ccgacctgcg ctgacgcttc attggcatgg tgaaccggat agccaaacgg
    19921 cgaccagagt ttatttttcg cgtagcccca gtactcttta cccaaattcc agcgttctgc
    19981 gatggcatat gaaccatcag ccaggtgact cagctcaccc acacgatgcg ccagacggta
    20041 gtaaaaatct ttaatgaagt taacgtcacc cgcttccagt tgatcccagc gaatttctgc
    20101 atactcttca gctggcagaa cacgcttgaa cacacctttg ctgtaacaat aagtaaagtc
    20161 gcgatgcaac tgcccgtagt tacaccatag gccgtagtca tcgaacaaat tcagaccaac
    20221 caggttacca atcacacgac catcgtcttt atcttcaaaa tctttcggcc cgttcgggaa
    20281 gatggtggta tggacaaagt ttgctacaca agtgttacca cctgtgctgg gcacgccaaa
    20341 ctctttcacc cgaggaatat tcatttgggt catacaacgg atcgggcaag agtggcagcc
    20401 gctcattttt actgtgtatt tctctgccgc cggtcctaag tcaaaaacgg atttataggt
    20461 acgaaagccg accgtattct gattgcctgg cggaatttca cccgtttcaa tcggaccacc
    20521 ttcagccgcg ccccaaaaca gccctttacg tgctgtccag cgtgacttgg gatctgaata
    20581 ctctgcccac gattgtggcg tacttggcac gacatggttg ttattcgcac caataagttc
    20641 agtcatcatg taatcattga gacgcttcat ctcctgacga tcggcaatgt tgaccccttt
    20701 cgtcccttca accgcaatcg ctttcaggtt tttcgaaccc attattgcgc cagttcccgc
    20761 accgccgctg tggttacggc tattcaacat gccagagaga ggaataaggt tttccccagc
    20821 ctgaccaata gccgccacac aggtttccgg gctggtcaat cgacaaattt cttccgtcgt
    20881 tgcgcgcgtc ccttttcccc ataagaaatc ggctttttcc aggctaactt tgtcatcttt
    20941 aatcttcagc cataccggtg atttcgcctt cccttcgata ataatgacgt cgtatccagc
    21001 gaatttcatt tgcgctgcaa aaaagccacc catatgggca tcgcgactaa atttcctttg
    21061 gtaaaagtag aaagtgaggt gatatttacg cgagaactac agggggcacc agatccaggt
    21121 aatgggccgg tagcaaagac taatttattc gcttcatcga aaggtttcgt gcctagcggt
    21181 acttcgtcat acataatttt gtagccgaag cccatgccac cgacaaaact tttaaactta
    21241 ctggaatctt cgagggtaat atttcctgtc gtgagattga ctcttaatat attacctgtc
    21301 caaccgttag ccatgatttt ttcctttgca agattcacac agtaatatct ttccactcga
    21361 taatttttaa cgcccctgtt gggcaggcgt ttgcgcattc accgcataac acacatttcg
    21421 aggatttttt actttcggta tttacggtgg ccatcatcca cgggcatgcc gtagtacagg
    21481 cgctacagcc aatacaacgt ttatgatcga cggtaataca gccttctttc tgctgccagg
    21541 taatcgcacc aatcgggcag acgttcatgc attgcggctc tttgcattga cggcaggtgt
    21601 ccgcggtata gttgagatcg ccatacaggc cgccgccaga gccaaccccg ttgtcgccaa
    21661 agaaataatt gcgatggatt ttgatacggg agaagaatgt ccctactgag ccatcgttga
    21721 agttggtaca agagatttca cagcggtggc aacccgtgca tcgcgctcgc tgggtcacca
    21781 gcaccccttt gggtgtattt atcaacccta ctgtgccgct atcaatatct tcctgtttgc
    21841 aacccaaaag tgacagtaac gcaggggcaa ttgttagacc agcaaggcct tttcctgata
    21901 tacgcaagaa ttctaatcgc gttaagccaa tatctaatag tggacgatca accgggttca
    21961 tttatttcgc atcctcttcg caaacacagt gctgacaatt cagcagcctg atattaagca
    22021 ttcaactaat acgctgctga ctctgtcccc aacaccagat attttcccta acccgatagt
    22081 gtatgagaaa agatttaacg ccgataaact aatcctttat ggttaggaga aaaaataatg
    22141 tgattattct gagcccttaa cattgatcgt tatcaattaa aattacaaag caagaaatta
    22201 taagcgaact gatatttatt atcatttgaa ataaatttaa cttaagcgac aatcatttgt
    22261 gacgttcatt gcagaaaata aaaataacat tatcgctata aattaaaata cataccgaaa
    22321 ttcgtataat ttcagcagtt gttatttaga atgattaact ggaccgcaac tgaagcaaga
    22381 atatcaggat gaagcttacc gcctcatcct gaatggtcat tttaccagtg cgttaatgac
    22441 ggtttccatc gcctgctttt ccagctcact gcgctgaatg cgtttattgg ctaatgctgt
    22501 acgtaatacg cctgcgataa caatatgctt tggttcctgc cccagatcgc gcatttccag
    22561 cacaacttta ccgacaactc tgcacatttc tcggtacagt tcatcctctt ttgtccgatt
    22621 tcccattctt tatacctatt tatcatgcca a
//
LOCUS       DAJTSA010000061        22412 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000061 DAJTSA010000000
VERSION     DAJTSA010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.061"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            82..2682
                     /locus_tag="OO369_002544"
     CDS             82..2682
                     /locus_tag="OO369_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCR5982776.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            2673..3656
                     /locus_tag="OO369_002545"
     CDS             2673..3656
                     /locus_tag="OO369_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5982777.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQHNIISPAGGTLPLSIPDDADSIRE"
     gene            3754..4294
                     /locus_tag="OO369_002546"
                     /pseudo
     CDS             3754..4294
                     /locus_tag="OO369_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4302..4817
                     /locus_tag="OO369_002547"
     CDS             4302..4817
                     /locus_tag="OO369_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5982778.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4783..5520
                     /locus_tag="OO369_002548"
     CDS             4783..5520
                     /locus_tag="OO369_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5982779.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5631..6641
                     /gene="pyrD"
                     /locus_tag="OO369_002549"
     CDS             5631..6641
                     /gene="pyrD"
                     /locus_tag="OO369_002549"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR5982780.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6815..7357
                     /gene="zapC"
                     /locus_tag="OO369_002550"
     CDS             6815..7357
                     /gene="zapC"
                     /locus_tag="OO369_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR5982781.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="OO369_002551"
     CDS             complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="OO369_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR5982782.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8707..10815
                     /gene="rlmKL"
                     /locus_tag="OO369_002552"
     CDS             8707..10815
                     /gene="rlmKL"
                     /locus_tag="OO369_002552"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR5982783.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10827..12734
                     /locus_tag="OO369_002553"
     CDS             10827..12734
                     /locus_tag="OO369_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5982784.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLNYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12864..14117
                     /gene="pqiA"
                     /locus_tag="OO369_002554"
     CDS             12864..14117
                     /gene="pqiA"
                     /locus_tag="OO369_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR5982785.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14122..15762
                     /gene="pqiB"
                     /locus_tag="OO369_002555"
     CDS             14122..15762
                     /gene="pqiB"
                     /locus_tag="OO369_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR5982786.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15759..16322
                     /gene="pqiC"
                     /locus_tag="OO369_002556"
     CDS             15759..16322
                     /gene="pqiC"
                     /locus_tag="OO369_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR5982787.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16578..16745
                     /gene="rmf"
                     /locus_tag="OO369_002557"
     CDS             16578..16745
                     /gene="rmf"
                     /locus_tag="OO369_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR5982788.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="OO369_002558"
     CDS             complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="OO369_002558"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR5982789.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17402..19162)
                     /locus_tag="OO369_002559"
     CDS             complement(17402..19162)
                     /locus_tag="OO369_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR5982790.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19347..19799
                     /gene="matP"
                     /locus_tag="OO369_002560"
     CDS             19347..19799
                     /gene="matP"
                     /locus_tag="OO369_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR5982791.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="OO369_002561"
     CDS             complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="OO369_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR5982792.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="OO369_002562"
     CDS             complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="OO369_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR5982793.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22005..>22412
                     /gene="sxy"
                     /locus_tag="OO369_002563"
     CDS             22005..>22412
                     /gene="sxy"
                     /locus_tag="OO369_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR5982794.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
BASE COUNT         5722 a         5258 c         5872 g         5560 t
ORIGIN      
        1 tatcgaacca tcaatgacta cggggcggaa acgccgcaac tcaactgtaa atcgtaagcc
       61 gtcttcagtt aagagagcga gatgtataga actcatcgac aacacagcct gttaagctct
      121 ggtggagtgc catcgtttat tggtgggctg gtggtgtttg tgtcggcagc gttcaatgca
      181 caagctgaaa cctggttcga tcctgctttt ttcaaagatg atccctcaat ggtggccgat
      241 ttgtctcgtt tcgaaaaagg acaaaaaata acgccagggg tttatcgtgt cgatattgtt
      301 ctgaatcaga caattgtaga tacgcgcaac gtcaattttg ttgagataac gccagagaag
      361 gggattgccg cctgtttgac gactgaaagc ctggatgcaa tgggtgttaa tactgatgcg
      421 tttccggctt ttaaacaact ggacaaacaa gtgtgtgtgc cattggcgga gattattccg
      481 gatgccagcg taacgtttaa tgtgaataaa ctccgtctgg aaatttcagt accgcaaatc
      541 gccatcaaaa gtaatgcccg tggttatgtc ccccctgaac gttgggatga agggatcaac
      601 gcgctattac tgggatattc gtttagcggg gctaacagta ttcatagcag cgcagacagt
      661 gattctggcg acagctattt tctgaattta aacagtggcg ttaatttagg cccatggaga
      721 ttacgcaaca attcaacatg gagtcgcagt agtggccaaa ccgcagaatg gaagaatctc
      781 agcagctatt tgcagcgggc ggttattcca ctaaaaggcg aactgaccgt aggtgatgat
      841 tatactgcag gcgatttttt cgatagtgtc agctttcgtg gtgtgcagct ggcgtcagat
      901 gacaacatgc tgccagacag cctgaaaggg tttgcgcctg tggtgcgtgg tatcgccaaa
      961 agcaatgccc agataacgat taagcaaaat ggttacacca tttaccaaac ttatgtatcg
     1021 cctggtgctt ttgaaattag tgatctctat tccacgtcgt cgagcggtga tttgttagtt
     1081 gaaatcaaag aagcggacgg cagcgtcaat agctacagcg taccgttttc cagcgtgcca
     1141 ttactccagc gtcaggggcg aatcaaatac gcggtgacac tggcgaaata cagaaccaat
     1201 agtaatgaac agcaggagag caaatttgcc cagaccacgt tgcagtgggg cggaccgtgg
     1261 ggaacgacat ggtatggtgg tggacaatat gctgaatatt accgtgccgc catgtttggt
     1321 ctgggattta accttggcga tttcggagca atttcgttcg atgcgaccca ggcgaagagt
     1381 acgctggcag accaaagcga acataaaggt cagtcatatc gttttctgta tgccaaaacg
     1441 ctcaaccaat tgggcactaa ttttcaattg atgggctatc gctattcgac gtcgggtttc
     1501 tacacccttt ccgacaccat gtataaacat atggatggct acgaatttaa tgacggtgat
     1561 gatgaagata cgccgatgtg gtcgcgttat tacaatttgt tttacaccaa acgtggcaaa
     1621 ctgcaggtca atatctccca gcaattaggc gagtacggtt cgttttattt aagtggtagc
     1681 cagcaaactt actggcatac cgatcaacag gatcggctat tacagtttgg ctacaacacg
     1741 caaattaaag atctctcgct gggggtttcc tggaactaca gtaagtcccg tggtcaacct
     1801 gatgctgatc aggtgtttgc actaaatttt tccctgccgc tcaatctgtt gctccccaga
     1861 agtaatgata gctataccag gaaaaaaaat tacgcctgga tgacctctaa caccagtatc
     1921 gataacgaag ggcacattac acaaaacctg ggtttaacgg agacactact cgatgacggt
     1981 aacctgagct acagcgtgca acagggatat aacagcgagg ggaaaacggc taatggtagc
     2041 gccagcatgg actacaaagg ggcgtttgca gatgcccgag tgggctacaa ctacagcgat
     2101 aacggcagtc aacaacaact gaactacgct ctttcaggca gtttagttgc ccattcacag
     2161 ggcattaccc tggggcaatc gctgggggaa actaacgtgc tgattgcagc accaggcgca
     2221 gagaatactc gtgtggcgaa cagcaccggg ctgaaaactg actggcgcgg atataccgtt
     2281 gtcccctatg ccacttctta tcgggaaaat cgaatcgcac ttgatgcggc atcgttaaaa
     2341 cgtaacgtgg atcttgaaaa tgcagtagtc aacgtggttc ccaccaaagg ggcgttggtt
     2401 ctggcggagt tcaatgccca tgcgggagca agggtattaa tgaaaacatc aaagcagggt
     2461 ataccgctgc gttttggcgc gatagcgacg ctggacggca tacagactaa tagcggcatt
     2521 attgatgatg atggctcgct ctatatgtcc ggtttgcccg ctcagggagc gataactgta
     2581 cgctggggcg aagctcccga tcaaatttgt catatcagtt accagcttac cgaacaacaa
     2641 attaactctg cgattacgcg aatggatgcc atatgcagat aatctttgga gaaaaatgcg
     2701 tgttattact acgactattt tttgccgccg tcttaatgct atggtgcgct caaactgctg
     2761 cttatatcgg gcagtgtcat accactcagg ggaatccgta tattggcgtc aattttggcg
     2821 ttaaaactct ggatgaagaa gaaaatacgg ctggagtagt taaagacaaa ttttatcagt
     2881 ggaacgaatc gaatgattat tatgtttcct gtgattgcga taaagacaat gtcagaagtg
     2941 gccgatgggc attcgccgcg gattcaccgt tagtctattt aggcgacaac tggtacaaaa
     3001 ttaatgacta tcttgccgcc aaagttttat tgcaggttaa aggcagttct cctactgcgg
     3061 ttcctttcga aaacgtgggc acaggggcag atacacgatg gcatatttgc gatcccggcg
     3121 gtcaacgttt aggtggccag ggggctagcg gtaatagcgg tagcttttcc ctgaaaatat
     3181 tgcagccgtt cgttggttcg gtcgtcattc ctcctatggc gctggcgcga ttatttgaat
     3241 gctacaacat acccgcaggt gattcctgca cgactactgg tacaccggtt ttagtgtatt
     3301 acctgtctgg tacgatcaat tcacttggct catgttccgt caatgccgga gaaacaatcg
     3361 aggttgatct gggcgacgta tttgcggcta actttcgtgt tgtagggcat aagcctcttg
     3421 gggccagaac ggcagaactt gcaattccgg tcaggtgtaa cacgggaaac gcagggttag
     3481 ttaacgtcaa tctgagtctg acggcaacct cagaccccag ctatccccag gcgattaaga
     3541 cgtcacgtcc tggcgtgggc gtggtggtga ccgatagcca gcacaacatt atttcccctg
     3601 ctggtgggac actaccgctc tctattcctg atgatgcaga cagtatccgc gaatgaatgt
     3661 ctatccagtc agcacgacaa gcgtaccacc agaaaccggg cgatttgaag ccacggcaac
     3721 ggtgagaata aattttgatt aacgcgtgaa cgtatgaaga aaaaaacgat atatcagtgc
     3781 gttattttgt tctttagcct tcttaacatc catgtcggga tggctgggcc tgaacaagtc
     3841 agtatgcata tttatgggaa tgtggtcgat cagggctgtg atgtcgccac caaaagtgca
     3901 ttacaaaata ttcatattgg tgattttaat atcagtgatt ttcaggccgc gaataccgta
     3961 agcactgctg ctgatttgaa tattgatatc accggttgtg ccgctggtat tactggcgcg
     4021 gacgtccttt ttagcggcga ggctgacacc cttgcgccga cactgctcaa actaactgac
     4081 acaggcggaa gcggtggtat ggcaacgggg attgccgtgc attcttgatg cgcaaagtca
     4141 gcaagaaatc ccgctcaatc aggttcagcc tcttacgccc ttaaaagccg gggataacac
     4201 actcaaatat caacttcgtt ataagtccac aaaggcggga gcaacgggcg gtaatgcgac
     4261 ggcggttctc tattttgatc tggtttacca gtgaaggagt gatgttgaaa cggataatct
     4321 ggattctgtt cttactggga ttaacggggg gctgtgagct atttgcccat gatggcacgg
     4381 tcaacattag cggatcgttt cgccgtaata catgcgtgct ggcacaggat agcaagcaaa
     4441 tcaatgtgca gttaggcgat gtctcgctga cacgtttttt tcatgggaat tatggcccgg
     4501 agaaatcttt catcattaat ttgcaggatt gcggaacaga tgtcagcacg gttgatgtca
     4561 ccttttcagg aacccccgac ggagtgcaga gcgaaatgct gtccattgaa agtggtacgg
     4621 atgcagccag tgggctggca attgcgattc tggatgatgc gaagatattg attccgctta
     4681 accaggcgag taaagactac agcctacata gcggtaaggt gccattaact ttttatgcgc
     4741 aattgcgacc tgtcaatagc gatgtgcagt cgggtaaagt gaatgccagc gcgacatttg
     4801 tacttcatta tgactaatac gtggaacaga ttagcgctct tgattttcgc cgttttatcg
     4861 ctgctggtgg cgggtgaatt gcaggcagga gtggtggtcg gcggaacgcg atttatcttt
     4921 ccggcagaca gagaatcgat atctatttta ctgactaata ccagtcagga atcttggctt
     4981 attaacagca aaatcaatcg cccaacgcgt tgggccgggg gggaagcgtc gacagtgcca
     5041 gcactattac tggccgctcc gccacttatt ctcctgaagc ccggtactac aggcacgttg
     5101 cgcttgctga gaacggaaag cgacatcttg cctgtggatc gcgaaacgct atttgagtta
     5161 agcattgcca gcgtgccatc cggcaaagtt gaaaatcaga gcgtaaaagt ggcgatgcgc
     5221 tcggtattta aactgttctg gcgacccgaa ggtttgccgg gcgacccgct ggaagcttat
     5281 caacaattac gctggacacg gaattcgcag ggtgtacaac tcactaaccc aacgccttac
     5341 tacattaacc tgattcaggt gagcgtaaat ggtaaagcgt taagtaatgc gggagtcgtg
     5401 ccgcctaaaa gccagcgtca gacaaactgg tgtcaggtaa tagcaccgtg tcatgtcgcc
     5461 tggcggacga taaatgatta cggcgggttg agtgcaaaga aggagcaaaa tctgccctga
     5521 aacaggttct gaaaacgttt gcgttttttt tgccgcaggt caattccctt ttggtccgaa
     5581 ctcgcacata atacgccccc ggtttgcaca ccgggaatcc aggagagttc atgtactacc
     5641 ccttcgttcg taaagccctt ttccagctcg atccagagcg cgctcatgag tttacttttc
     5701 agcaattacg ccgcattacc ggaacaccgt ttgaagcact ggtgcggcag aaagtgcctg
     5761 cgaaacctgt taactgcatg ggcctgacgt ttaaaaatcc gcttggtctg gcagccggtc
     5821 ttgataaaga cggggagtgc attgatgcgt taggcgcgat gggatttgga tcgatcgaga
     5881 tcggtaccgt cacgccacgt ccacagcctg gtaatgacaa gccgcgtctc tttcgtctgg
     5941 tagatgccga aggtttgatc aaccgtatgg gctttaataa tcttggcgtt gataacctcg
     6001 tagagaacgt aaaaaaggcc cattatgacg gcgtcttggg tattaatatc ggcaaaaata
     6061 aagatacgcc agtggagcag ggcaaagatg actatctgat ttgtatggaa aaaatctatg
     6121 cctatgcggg atatatcgcc atcaatattt catcgccgaa taccccagga ttacgcacgc
     6181 tgcaatatgg tgaagcgctg gatgatctct taaccgcgat taaaaataag caaaatgatt
     6241 tgcaagcgat gcaccataaa tatgtgccga tcgcagtgaa gatcgcgccg gatctttctg
     6301 aagacgaatt gatccaggtt gccgatagtt tagttcgcca taatattgat ggcgttattg
     6361 caaccaatac cactctcgat cgttctctgg ttcagggaat gaaaaattgc gatcaaaccg
     6421 gtggcttaag tggtcgtccg cttcagttaa aaagcacaga aattattcgc cgcttgtcac
     6481 tagaattaaa cggtcgctta ccgatcatcg gtgttggcgg catcgactcg gttatcgctg
     6541 cgcgtgaaaa gattgctgcg ggtgcctcac tggtgcaaat ttattctggt tttattttta
     6601 aaggtccgcc gctgattaaa gaaatcgtta cccatatcta attatttctt cgaattcctc
     6661 tttataagat aatcagggct ttattttcag acctggttgt tttatattca tcgttgttgc
     6721 ttatttagac attttgtact tttattgttg aggttattaa gcgaagcgac aatggattgt
     6781 gttgctgcgg ttttataggg taggggagag gcagatgcga attaaaccag acgataactg
     6841 gcgttggtat tacgatgaag agcacgatcg aatgatgctc gatttagcca atggtatgct
     6901 atttcgctca cgttttgcgc gcaaaatgtt gactccagat gccttttctc ccgcaggctt
     6961 ttgtgttgat gatgccgcgc tctatttctc ttttgaagaa aagtgccgcg attttaattt
     7021 atctaaagac caaaaagccg aactggtgct gaatgcactg gtagcaattc gttatctcaa
     7081 accgcaaatg ccgaaaagct ggcattttgt ctcccatggt gaaatgtggg tgcccatgcc
     7141 gggcgatgcg gcctgcgttt ggttgagcga tactcacgag caggtcaatt tgctggtcgt
     7201 tgaatctggc gaaaatgccg cgctatgcct gctggcacaa ccctgcgttg ttattgcggg
     7261 tcgcgcgatg cagttgggcg acgccattaa aatcatgaac gacaggctga aaccgcaggt
     7321 gaatgttgac agcttcagcc tcgaacaggc agtctaacgc gtcagcttaa gggcagtctt
     7381 cggtacacag ctacagcaaa gaatggtgcc atcatcgccc attgctgatt ttttcagcgg
     7441 cgtgacttcg ccttctaaaa gctgaacacg gcaacttcca caaatgcccg cgcggcaaga
     7501 ataagggata cgaattccct gattttctaa ttgctccagc aacacttgtt ggttatttcc
     7561 acgaaatgcc tgtccctgcc agtcaatatc tacatttgcg tccggttgtt gcgtgatgtt
     7621 ggcggtatca tcagcggcag ctgcgccgta aatttttgcc ggagccgttg ccagaatttc
     7681 cacctcatcg ccaacgcgaa tcacgccgct attacgggca attaaattct gaccaaaatc
     7741 gacatcgccg ttatcctggg cagtgcggaa agattgcaat gtttttaatg gttcgcctgc
     7801 cggatgtttt tgcccttttt ctgggctgac ggtggtgaaa atacagcggc tacaaggttt
     7861 aaccacatca aacaccacat caccaatgcg aatcactttc cagctatctt cttcccaggc
     7921 tgacgcgccg gaaaccacca gattggggcg gaattgctcc atttttacac tggccggaca
     7981 acgttgttgg agatcacgta acgaggcttc gttagcaaga aggtaaggat agccatcagc
     8041 aaatgacaga ggtacagtgt tgtggcgttt cacgcgccgg gtcatttgtg gccccaccca
     8101 gcgtaattgc acttcgcggg agaaaaatcc acttagccat ttgttgatcg cgtctggcgc
     8161 aattcgcgcg gtaaaatgtg tgccccaaac ttcggttggt gcgtcttgtg tggcgaaatc
     8221 agcaaaacga acatatgcgc tactgccatc tggtgcggtg agatgcaagc catcatgcac
     8281 aggtgaagga gtaaaccgta ccatctgggg aaactggcga gcggtaataa acgtaccgtc
     8341 aggttccgtg atcataaaga tgcgatcgaa ggccagacca ctgacatcag ccagagcatg
     8401 tgtaagacca atgccgcgca tcgatttaac aggatgaata aaaagccggg ttaatgtcgc
     8461 cacggtgcgg tcctcaaatg aaaataagcc ctcaacttta tgacatgacg cgcttattag
     8521 ctataatgcg caacaatttt cttagcgcct gtcccgttag gctattttat ttgtcagttt
     8581 ggccctgggt tgtgctcgaa atcctcacgt acttgtgtac gctccggttt ctccgcgctc
     8641 tccatgtcca gactgactgc aacaattacg cctactgcgg taggctctta gagtaaaagt
     8701 gacgatatga attctctgtt tgccagtacg gcccgtgggc tggaagagct gttaaaaact
     8761 gaactggaaa atctgggggc cgttgaatgc caggtggttc agggtggggt ccatttcaag
     8821 ggcgacacac ggcttgttta ccagagcctg atgtggagcc gcctggcctc gcgtattatg
     8881 ttgccgctgg gcgagtgtaa ggtttacagc gatttagacc tctatctcgg tgttcaggcg
     8941 atcaactgga cagagatgtt taatcctggc gcgaccttcg ctgtccactt cagtggtttg
     9001 aatgacacca tccgcaacag tcagtacggc gcgatgaaag tgaaagacgc gatcgtcgat
     9061 gctttcacac ggaaaaatct gccgcgtcca aatgttgatc gcgatgcgcc ggatatccgc
     9121 gttaacgtct ggctgcataa agaaaccgcc agtatcgctc ttgatctgag tggtgatggt
     9181 ttacatctgc gtggctatcg cgatcgtgct ggtattgcgc cgatcaaaga aaccctggca
     9241 gccgctattg tgatgcgatc cggctggcag tcaggaacac cgctgctcga tccgatgtgt
     9301 ggttccggta cgttgctgat tgaagcggcg atgctggcga ccgatcgcgc accaggcttg
     9361 caccgtgggc gttggggctt tagcggctgg gcgcagcatg atgaagctat ctggcaggaa
     9421 gtgaaagcgg aagcgcaaac tcgcgcccgt aaaggcctag ctgagtatag ctctcatttt
     9481 tacggttcgg acagcgacgc acgggtgatt caacgtgcgc gcactaacgc ccgtcttgcg
     9541 gggattggtg aactgatcac ctttgaggtg aaagatgtcg cgcaactgac caatccgctg
     9601 ccgaaagggc cgtacggtac agtgttgagc aacccgccat acggtgaacg tctggacagc
     9661 gaaccggcgc tgattgcgct gcatagtctg ctgggccgaa tcatgaaaaa ccagttcggt
     9721 ggctggaatc tctctttgtt tagtgcctcg ccggatctgc taagctgcct gcagctgcgt
     9781 gcagacaaac agtacaaggc gaaaaacggc ccgctggact gcgtacagaa aaattaccac
     9841 gttgccgaaa gcacaccaga cagtaaaccg gcaatggtag cggaagatta cgccaaccgc
     9901 ctgcgtaaga acctcaaaaa attcgagaag tgggcacgcc aggaagggat tgaatgttac
     9961 cgcctgtatg acgccgatct gccggaatat aacgttgccg ttgaccgtta tgccgactgg
    10021 gtggtggtgc aggagtatgc gccgccaaaa actattgatg cgcacaaagc gcgtcagcgt
    10081 ctgttcgata ttatcgctgc aaccatttcg gtactgggga ttgcgccaaa caaactggtg
    10141 ctgaaaaccc gtgaacgcca taagggcaaa aaccagtacc agaaactggg cgagaagggc
    10201 gagtttcttg aagttaccga atataacgct cacttgtggg tgaacctgac ggattatctc
    10261 gatactggtc tgttcctcga tcaccgtatc gcccgccgta tgctcggtca gatgagcaaa
    10321 ggcaaagatt tcctcaacct gttttcttat accggcagcg ccaccgtgca tgcgggatta
    10381 ggcggtgcac gcagcaccac caccgtggat atgtcgcgta cttatctgga atgggcagaa
    10441 cgcaacctgc gtctgaatgg cctgactagg cgtgcgcatc gcctgattca ggccgattgc
    10501 ctggcgtggc tgcgtgaggc aaatgaacag ttcgatctga tctttatcga tccgccaacc
    10561 ttctctaact caaaacgaat ggaagatgcg tttgatgttc agcgcgatca tctggcgctg
    10621 atgaaagatt tgaaacgtct gctgcgtgca ggtgggacga tcatgttctc gaacaacaaa
    10681 cgtggcttcc gtatggatct cgacggcctg gcaaaactgg gactgaaagc acaagaaatt
    10741 acgcaaaaaa cgctctccca ggatttcgcc cgtaaccgcc agatccacaa ctgctggctg
    10801 attaccgcag cctgaaagga atagtaatgt cattaatcag tatgcatggc gcatggctgt
    10861 cgttcagcga cgcgccgctt ctcgataacg cagaactgca tatcgaagat aacgaacgtg
    10921 tttgtctggt gggccgcaac ggcgcaggaa aatcgacgtt aatgaaaatc ctcaaccgtg
    10981 aacaagggct ggatgacggt cgcattattt atgagcaaga tttgattgta gctcgtctgc
    11041 aacaggatcc gccgcgtaac gttgagggta gcgtttatga tttcgttgcc gaaggcattg
    11101 aagaacaagc ggaatatctg aaacgctatc acgatatttc gcgcctggtg atgaacgacc
    11161 cgagcgagaa aaatctcaac gaactggcga aggttcagga acagctggat caccacaacc
    11221 tgtggcagct ggaaaaccgc atcaacgaag tgctggcgca actggggtta gatcctaacg
    11281 ttgcgctgtc gtcgctttcc ggcggctggt tgcgtaaagc ggcattagga cgcgcgctgg
    11341 tgagcaatcc gcgcgtattg ctgctggacg aaccgactaa ccatctggat atcgaaacca
    11401 tcgactggct ggaggggttc cttaaaacct tcaatgggac gattattttc atctcccacg
    11461 accgttcgtt tatccgcaat atggcgacgc gcattgttga tctcgatcgc ggcaagctgg
    11521 tgacctatcc agggaattac gaccagtacc tgcaggaaaa agaagaagcc ctgcgcgtgg
    11581 aagaactaca aaatgccgag ttcgatctca aactggcgca ggaagaagtg tggattcgcc
    11641 aggggatcaa agcacgccgt acccgtaatg aaggccgcgt acgcgccctg aaagcgatgc
    11701 gtcgcgaacg tggtgaacgt cgcgaagtga tgggtaccgc aaagatgcag gtggaagagg
    11761 ccagccgctc cggtaagatc gttttcgaaa tggaagacgt ttgctaccag gttaacggta
    11821 agcaactggt gaaagatttt tctgcccagg ttctacgtgg cgacaaaatt gccctgattg
    11881 gtccgaatgg gtgcggcaaa accacgctgc tgaaactgat gctcggtcag cttcaagcgg
    11941 acagcgggcg tattcacgtt ggcaccaaac tggaagtggc ttatttcgat cagcaccgcg
    12001 cggaactgga tcccgataaa acggtgatgg ataaccttgc cgaaggtaag caagaggtga
    12061 tggttaatgg caagccacgc cacgtattgg gctatttgca ggactttctg ttccatccga
    12121 aacgggcgat gacgccagta cgtgcgtttt ctggcggtga gcggaaccgc ttgctgctgg
    12181 cgcgtttgtt cctcaaacca agcaacttat tgattcttga cgaaccgacc aacgatcttg
    12241 atgtcgaaac gctggaactg ctggaagaac tgatcgacag ctatcagggc acggtattgc
    12301 tggttagcca cgatcgtcag tttgtcgata acaccgttac agaatgctgg atcttcgaag
    12361 gcggcggtaa aattggtcgt tatgttggcg gttatcatga tgcccgtggt cagcaagaac
    12421 aatatgtggc actcaaacag cctgcggtga aaaaaagcga agaagccgcc gcgccaaaag
    12481 cagaaactgt aaaacgcagc agtagcaaac taaactataa attgcagcgc gaactggagc
    12541 agctaccgca attgctcgaa gatctggagg cgaagctgga agccctacag acgcaagtgg
    12601 cggatgcttc cttcttcagt cagccgcatg agcagacgca aaaagtgctt gctgatatgg
    12661 ctgctgcaga gcaggagctg gagcaagcct ttgaacgctg ggagtatctt gaagcgttaa
    12721 aaaatggtgg ctgatcgcaa aatagtcgat ttaaacagag acagctgaca acggtaaaat
    12781 tgcctgatga actgggttta tcaggcatga aaaccaacac tttgttagtc atctgtaaaa
    12841 tagcgcatca ttaaggagta ccaatgtgcg aacatcatca tgccgcgaag cacatcctgt
    12901 gctcgcagtg tgacatgctg gtggcgttac cgcgccttga gcatggtcag aaagcggcat
    12961 gtccccggtg tggcacaacg ttaaccgtgg cgtgggatgc ccctcggcag cgtcccaccg
    13021 cctatgcgtt ggctgcactg ttcatgctgt tgctgtccaa cttgtttcct tttgtgaata
    13081 tgaacgttgc aggagttacc agtgaaatta cattactgga aattcccggc gtgctttttt
    13141 ctgaggacta cgccagcctc ggcacctttt tcctgttgtt tgtgcaactg gttcccgcgt
    13201 tttgtctgat aaccattctg ttactggtga atcgcgcgga attaccggtc cgtttaaaag
    13261 agcaactggc acgggtgctt tttcaactca aaacctgggg aatggcggag attttcctcg
    13321 cgggtgtgct ggtcagtttc gttaaactga tggcttacgg cagcattggg gtaggcagca
    13381 gctttctccc ctggtgttta ttttgtgtcc tgcaactgcg cgcttttcag tgcgttgatc
    13441 gtcgctggtt atgggacgac atcgccccga tgccagaact gcgccagccg ctaaaaccag
    13501 gcgtcacggg gatacgtcag gggctgcgtt cgtgctcctg ttgtacggca atccttcctg
    13561 ctgatgaacc cgtgtgcccg cgttgtagta ccaaagggta cgttcggcgt agaaacagcc
    13621 tgcagtggac actcgcgctg cttgtaacgt ccatcatgct gtatcttccg gctaatattt
    13681 tgcccatcat ggtgacggat ttattaggct cgaagatgcc gtcgacgatt ctcgctgggg
    13741 tcattctgtt atggagcgaa ggctcttatc ccgtcgctgc ggtgatcttt ctggccagta
    13801 ttatggtgcc aacgttaaag atgatcgcca tcgcgtggct gtgttgggat gccaaagggc
    13861 atggcaagcg cgacagtgaa agaatgcatt tgatttatga agttgttgag tttgtaggcc
    13921 gctggtcgat gattgacgtt ttcgttatcg cggtgctctc ggcgctggtg cgtatgggag
    13981 gtttaatgag tatttatccg gcaatgggtg cattaatgtt tgctttagtc gtcataatga
    14041 caatgttttc tgctatgacg tttgacccgc gtttgtcgtg ggatcgtcaa cctgaatcag
    14101 agcatgagga gtcctgacag tatggaatct aataatgggg aagccaaaat ccagaaagtg
    14161 aagaactggt ctcccgtgtg gatatttcct atcgtcacgg cgctcattgg ggcctgggtt
    14221 cttttttatc attacagcca tcagggaccg gaagtgaccc tgatcaccgc gaatgcggaa
    14281 ggaattgaag gtggcaaaac caccattaaa agccgtagcg ttgacgtcgg cgtggttgaa
    14341 agcgccacac tggctgatga tttgacgcac gttgaaatca aagcgcggct gaattccggt
    14401 atggaaaaat tgctgcataa agacaccgtc ttttgggtgg tgaaaccgca gattggtcgc
    14461 gaagggatta gcggcctggg aacgctgctg tctggagttt atatcgaact gcagccaggc
    14521 gcgaaaggca gcaaaatgga taaatacgat ttgctggact caccaccgtt ggccccgcct
    14581 gatgcgaaag gtatccgtgt gattctcgat agcaaaaaag ccgggcagct ctcgccagga
    14641 gatccggtgc tgttccgtgg ctatcgggta ggttcggttg aaaccagcac cttcgatacg
    14701 caaaaacgca atatcagtta tcaactgttc atcaatgcac cttatgaccg actggtgacc
    14761 agcaatgttc gcttctggaa agatagtggc attgcggttg atctgacgtc agcggggatg
    14821 cgtgtggaga tgggctcatt gacaacgctg cttagtggcg gtgttagctt tgatgtgccg
    14881 gaaggtctgg atttagggca gccagtggca ccgaaaacag ctttcgtttt gtatgatgat
    14941 cagaagagca ttcaggattc gttgtacacc gatcacattg attatctgat gttctttaaa
    15001 gattcggtac gcggtctgca accgggagct ccggtagaat tccggggtat tcgcctgggt
    15061 accgtaagca aagtgccatt ctttgcgccg aatatgcgtc agacatttaa cgatgattac
    15121 cgtattccgg tactgattcg tatcgagcca gagcggctga aaatgcagct tggcgaaaat
    15181 gcggatgttg ttgagcacct tggcgaattg ttgaaacgtg gtttacgcgg atcgctgaaa
    15241 accggaaacc tggtcactgg tgcactgtat gttgatctcg atttctatcc aaatacgcct
    15301 gcaataaccg gtattcgtga atttaatggt tatcagatta tcccgactgt tagcggcggc
    15361 ttggcgcaaa tccagcaacg actgatggaa gcgttggata agatcaacaa actgccattg
    15421 aatccgatga ttgaacaggc aaccagtacg ctttctgaaa gtcagcgcac aatgaaaaac
    15481 ctgcaaacga cgctggatag catgaacaag atcctcgcca gccagtcgat gcagcagttg
    15541 ccgacggata tgcagtcaac gttgcgtgaa ttgaatcgca gcatgcaggg cttccagcct
    15601 ggctccgcag cctacaacaa gatggtggcg gatatgcagc gccttgatca ggtgttgcga
    15661 gaactgcaac cggtgctgaa aacgctcaat gagaagagta acgcgctggt atttgaagcg
    15721 aaggacaaaa aagatccaga gccgaagagg gcgaaacaat gaaaaagtgg ctagtgacga
    15781 ttgcagcact gtggctggcc ggatgcagct ccggcgaaat taataaaaac tattaccagt
    15841 tacctgtggt gcagagcggt acacaaagta ccgccagcca gggcaatcgt ctgttatggg
    15901 tagagcaggt cactgttcct gactatctgg cggggaatgg tgtggtttat caaaccagtg
    15961 atgtgaagta tgtgattgcc aacaacaact tgtgggccag cccgttggat caacagttgc
    16021 gcaacaccct ggttgccaac ctgagtacgc aactgcccgg ctgggtggtt gcctcccagc
    16081 ctctgggaag cgcccaggac acgctcaatg ttaccgtaac ggagtttaac ggtcgctatg
    16141 atggcaaggt cattgtcagt ggtgagtggc tgttgaacca ccagggacaa ctgatcaaac
    16201 gtccgttccg tctggaagga gtgcaaactc aggatggtta cgatgagatg gttaaagtgc
    16261 tggccggtgt ctggagtcag gaagccgctt ctattgcaca agagataaag cgtctacctt
    16321 aattataaag atttgtaaat ataaccgtct ccggtatgtt gcctgaggcg gtttttttgt
    16381 ttctaacgtg cggaaaaatt tgttcctctt cacatttttt gtacaaccga catgcccgtg
    16441 tagctcacaa atatgacagt ggcgtgaatt ttgcgcattg acggcagtta tgattcgcgg
    16501 tattgcttaa ctgtgattgc acatttagta atcactgttt tcttttccac cagaaaccag
    16561 tatgagggaa acgaggcatg aagagacaaa aacgagatcg cctggaacgg gcacatcaac
    16621 gtggttatca ggccggcatc gccggacgct caaaagaaat gtgtccctat cagacgctga
    16681 gtcaaaggtc acaatggctg ggaggctggc gagaagccat ggcggacagg gtagtaatgg
    16741 cctgattctg tctctttaaa aagaaacctc cgcattgcgg aggtttcgcc ttttgatact
    16801 ctgtctgatg ataatcagaa ggcagacgta tcctggaaca gaccgacttt caggtcgctg
    16861 gcggtataga tcagacgaca atcaaccagc acttcgccat ctgccagacc cataatcaga
    16921 cgacgattaa caatgcgttt aaagtgaata cggtaggtca cttttttcgc tgtcggcagt
    16981 acctgaccag tgaatttcac ttcgccaacg cccagcgcgc ggcctttacc ttcgccgccc
    17041 agccagccga ggtagaaccc taccagctgc cacattgcgt ccaggcccag gcatcccggc
    17101 ataaccggat cgccaatgaa gtggcatccg aagaaccaca gatccggatt gatatccagt
    17161 tctgcttcaa catacccttt gtcgaagtta ccacccgttt cggtcatttt gaccacacgg
    17221 tctatcatca gcatgttcgg tgctggcaat tgcgggcctt tagcgccaaa cagttcaccg
    17281 tgaccagagg caagaaggtc ttcttttgta taggattcgc gtttatctac catgttctct
    17341 gtaagcctta ttttattgaa gcacgcagga tagctaacac gtgtacgctg aacaagtccg
    17401 atcagttcgg aataaaccag ttcagccaac gtaatggcca tggaaaacgg tgacgtcctt
    17461 cctgttgcga tgcttgcgcg atacgttcct ggatggtttg catcagcgtc gtttggcctt
    17521 cgccatccca caccagattt aataataacg gcagtgcgtc agtcacatcg tctactgccc
    17581 agatggtgaa tttgccttct tctaccgctt tcaccagttc actgtgaaga cttaaatggc
    17641 gaacgttagc agtggggata atgacacctt gtttccccgt taactcacgt tgctggcaaa
    17701 tagcaaagaa gccttcgatt ttctcattta aaccaccgac cggctggacg cggccgaact
    17761 gatcgactga acctgtgata gcgatactct gattcactgg cacatcagcg agggcgctta
    17821 tcagggcgca gagttcagcc atcgaggcac tatcgccatc cacttcactg tatgactgct
    17881 caaatgtcag cgatgctgag aaggggatct gttgctcaag ctgtagttcc gacatcagga
    17941 acgcctgcat gatcatcatc cctttcgcat ggatattgcc gccaagctcc gctttgcgtt
    18001 cgatgtcggt gaattcacca tcgccaatat gcacaacgca gctaatgcga gaaggttcgc
    18061 caaaagcgcg tggatgaccc ggaaattcaa tgaccgaaag ggcgttaatt tgcccgatgc
    18121 gttcgccttc ggtttcaatc aggatttgct cctgaaggat ctcatcctgc atacgttcag
    18181 cgaggaaacc ttcgcgccat tcacgctgct gcagcattaa gtttagctgc tcgccggaga
    18241 aggtgtcgcc atcacacagg gaggcgacct ctttacactg gcggaggatc cactgcgggc
    18301 taagcggaag tgtttcttgt tcaccggtgt agcgtgctgc ttcgcggata agtaccggcc
    18361 aagcatccgc tcccggggca ggtaagtgat tatgtctggc ggtaaatgtc acccagcgac
    18421 accactggct tactgactcc gcatcgacaa tctgcagagt atcttcaaat tcgctataaa
    18481 tagcctgctc tgaaagctct ggctccatct cctggaaatc agccaatgat tcgcgttcgc
    18541 ctaccagaat gactttcagc ttcaatggca ttgaaggcac agagacgggg agagggcgcg
    18601 actcatcaaa cgcaacccag tcaaaacgct cgcggttaac gatatttttc agccgcatcc
    18661 acagcagagg ttgcgccagc agtgtacgca aagagatgat gagaataccg ccatttgcct
    18721 gatgcaccag accaggctgc agggtaatgt cgccattaaa ctggcgcagg cagccaaaga
    18781 gttgctccgc ttctacccag tcggcagcga caacttgcgt taaagtcgca aaattatcat
    18841 ctgcactcac tgcgtgacgt aagcggatgg agtggccgga aacctcatag tgatcgccca
    18901 ccagttggcc tgcatcgctt tgtagcgtac gcgcggcatc ggcaataaga ttcagatact
    18961 cagactcttc cggggcctta gccagcataa aagaggagga tgctcgcgta tgcagcaact
    19021 gctccagcgc aaattgcagt cgcggttgag tatcactgaa taaaggatcg ttttcgtcaa
    19081 tcaaatgtgg ctgagcaaat atttcctgat agctatcggt atcaggaacc aggtcacgcc
    19141 atgcaagttt cgtaatggtc aaagttgatg ttttttagtc tgttgtcaaa gccgcgatta
    19201 taccgtaacc ggcactacag cacacgtaga aagcaccgac aatactcctg gctgggcgtt
    19261 aaagctcaca ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag
    19321 agttacacgg taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg
    19381 gttggaaatg gaagtatctg gtgaagaagc atcgcgaagg ggagttaatt acccgttaca
    19441 tagaagccag tgccgcccag gaagccgtcg atgtgttgct ctcgctcgaa aatgaacccg
    19501 tgctggtaaa tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc
    19561 agaccattcg ggccagacgg aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa
    19621 aatcgatcga tctggaattt attgtctggc aacgtctggc tggtcttgca cagcgtcgcg
    19681 gtaaaacgct ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat
    19741 acgcgaataa aatgtcttct cttaagcagg atctgcaggc attgctgggt aaggaataac
    19801 tgacgaaagt cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc
    19861 taccagacga taacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg
    19921 atctctacgc gacgatccgg agccaagcag tcgatcagtg cagcacgctg tttcacgttg
    19981 tcacaggtgt tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct
    20041 gccgggatac ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga
    20101 ccctggttgt aagcgtcaga accgatgcgg tcagtgtaac ccagaacaac tacggaaccg
    20161 tctttcggat ctaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc
    20221 ggtttcaggg ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg
    20281 gtctgtactt ccggtgccgg agctggagca actaccggag ctgcttcgcc ctgaccgaaa
    20341 cggtaggaaa cacccaggct cagcagaccg ttgtccggac gagtaccgat ggtgtttgcg
    20401 tcaccgatgt tgttggtcca ctggtattcc agacgggtag cgatttcagg agtgatcgcg
    20461 tactcaacac cacctgcgaa aaccggagaa acgccagtgt cgtggtcttt aaaggatgcg
    20521 ccaccaggta cgttagcctt ggtgtctgca cgccatacca taccacccag acgagtgtag
    20581 atgtccagat cgtcagtgat tgggtaaccc agtttagcgg tcagctgaac gccctgagct
    20641 ttgtatgcgc cgttgatgtt gtcgcctttg tacggcatac gacctaacca gtcgtaaccc
    20701 atttcaaaac caacatacgg gttaacctgg taaccaccaa aagcacctgc acccagttgg
    20761 ttttcgtggg tcggaccatt gttaggaata aaaccagtgt catggtactg ggaccagccc
    20821 agtttagcac cagtgtacca ggtgttatct ttcggagcgg cctgcgctac ggtagcgaaa
    20881 ccagccagtg ccactgcaat cgcgatagct gtctttttca ttttttgcgc ctcgttatca
    20941 tccaaaatac gccatgaata tctccaacga gataacacgg ttaaatcctt caccggggga
    21001 tctgctcaat attaactcta ccgatatctt cggcttatgc cgagcacccc tggcgatgta
    21061 aagtctacaa cgtagttgaa aacttacaag tgtgaactcc gtcaggcata tgaaaaaaaa
    21121 gtcttgtata aggtatgttt aatctttttg tcagcgacaa tttacagaaa aaaaacgcgg
    21181 aaaccgcttc agacaagcct tcgcaaggaa aattagtcac gactgaaagc attggctggg
    21241 cgacaaaaaa agttctagga ttaatcctaa atttacttaa tgatacaaat tagagtgaat
    21301 ttttagcccg gaaagttgtc tcgttgtgtg agaggatgcg cttaccggac gcataataaa
    21361 ccccatagcg ttaccttcat ttgccgcatc aacaagttca gcatgctctt cttcagtcaa
    21421 atcatctgtc aaccaaccga tcaccacact gtaattgccc gtgcgtaaag cgcgaaccat
    21481 tgactccact gtgtggcaag gggagagctg gctaatctgc attactttcg ttaagggtag
    21541 cccagatgcc tgaacccatt cccgactcag tttttgttgc ggtgttaacc agagttgcca
    21601 gcgcgattgc tgaccgagtt gctgtaacaa tggcaacagt agaagttgcg tcatcatggg
    21661 ctgatcttcg cgatagacaa cttcactgat aagcccggct gtagtgtttt ccgtagagac
    21721 acgcgcaatt ttacttgctg cggatgagaa cgacgaagaa cgatgtgcat agcctgaagt
    21781 gtacataatc aatccagccc ctgtgagtta ctgtatggat gtgcagtaca tccagtgaca
    21841 acaaagatca accctatttt cggaaagagc ctcgcaaatt ttgtcgttgg tgacgggaaa
    21901 acataaatta atcttgcccc ttaagaataa gttgcctatt ttcgtagtta acggatccgt
    21961 taatgtgaat cattctttta tgttatgatt ttaaaaggaa ttttatgaaa agcctctcct
    22021 ataagcggat ctataaatca caagaatacc tggcaacgtt gggcacaatt gaataccgat
    22081 cattgtttgg cagttacagc ctgaccgttg acgacacggt gtttgcgatg gtttctgatg
    22141 gtgagttgta tcttcgggct tgtgagcaaa gtgcacagta ctgtgtaaaa catccgcctg
    22201 tctggctgac atataaaaag tgtggccgat ccgttaccct caattactat cgggttgatg
    22261 aaagtctatg gcgaaatcaa ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag
    22321 cgctgaaaga gaaaagcacg cgcaataccc gggaaagact gaaagatttg cccaatatgt
    22381 cttttcatct ggaagcgatt ctcggggagg tg
//
LOCUS       DAJTSA010000062        22301 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000062 DAJTSA010000000
VERSION     DAJTSA010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.062"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1025)
                     /locus_tag="OO369_002564"
     CDS             complement(<1..1025)
                     /locus_tag="OO369_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5982795.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEA"
     gene            complement(1127..2107)
                     /locus_tag="OO369_002565"
     CDS             complement(1127..2107)
                     /locus_tag="OO369_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR5982796.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2115..2825)
                     /locus_tag="OO369_002566"
     CDS             complement(2115..2825)
                     /locus_tag="OO369_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR5982797.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3042..4865)
                     /gene="typA"
                     /locus_tag="OO369_002567"
     CDS             complement(3042..4865)
                     /gene="typA"
                     /locus_tag="OO369_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR5982798.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5238..6647
                     /gene="glnA"
                     /locus_tag="OO369_002568"
     CDS             5238..6647
                     /gene="glnA"
                     /locus_tag="OO369_002568"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR5982799.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6820..7869
                     /gene="glnL"
                     /locus_tag="OO369_002569"
     CDS             6820..7869
                     /gene="glnL"
                     /locus_tag="OO369_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR5982800.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7881..9290
                     /gene="glnG"
                     /locus_tag="OO369_002570"
     CDS             7881..9290
                     /gene="glnG"
                     /locus_tag="OO369_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR5982801.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9402..9512
                     /locus_tag="OO369_002571"
     CDS             9402..9512
                     /locus_tag="OO369_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR5982802.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="OO369_002572"
     CDS             complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="OO369_002572"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR5982803.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="OO369_002573"
     CDS             complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="OO369_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR5982804.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            12394..13026
                     /locus_tag="OO369_002574"
     CDS             12394..13026
                     /locus_tag="OO369_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR5982805.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13408..16194)
                     /gene="polA"
                     /locus_tag="OO369_002575"
     CDS             complement(13408..16194)
                     /gene="polA"
                     /locus_tag="OO369_002575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR5982806.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16558..17490
                     /locus_tag="OO369_002576"
     CDS             16558..17490
                     /locus_tag="OO369_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5982807.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17531..18961)
                     /locus_tag="OO369_002577"
     CDS             complement(17531..18961)
                     /locus_tag="OO369_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5982808.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(19116..19742)
                     /gene="dsbA"
                     /locus_tag="OO369_002578"
     CDS             complement(19116..19742)
                     /gene="dsbA"
                     /locus_tag="OO369_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR5982809.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19759..20745)
                     /gene="srkA"
                     /locus_tag="OO369_002579"
     CDS             complement(19759..20745)
                     /gene="srkA"
                     /locus_tag="OO369_002579"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR5982810.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20822..21091)
                     /locus_tag="OO369_002580"
     CDS             complement(20822..21091)
                     /locus_tag="OO369_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR5982811.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            21161..21745
                     /gene="mobA"
                     /locus_tag="OO369_002581"
     CDS             21161..21745
                     /gene="mobA"
                     /locus_tag="OO369_002581"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR5982812.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21727..22254
                     /gene="mobB"
                     /locus_tag="OO369_002582"
     CDS             21727..22254
                     /gene="mobB"
                     /locus_tag="OO369_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR5982813.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5517 a         5592 c         5339 g         5853 t
ORIGIN      
        1 gcttcgccga taggtgcaaa aaatacggct cgctgtgtaa agactatcgc gccaaagccc
       61 agcgtacatg ccatccctaa atagaccggc atactttcgt gcggcagcat aatcaacagt
      121 acgagcgcag cggtactgat gataaaggtg tagcatagat atttactcgg cgatttcagg
      181 atcttatctg aaatcatgcc accaatcggt ccaccaatca ttttcagaca gtattggtta
      241 atgatgccgt aagcccccac cagcgcaacg ggcaatagat agatgttttt caggaatgga
      301 atgaagaatg tcaggccaca gtaaaccgcg taaacgaaga agacgttgaa agcgataagc
      361 cagatggttt tgtctctcag cactgaggtc atactggtgt tttgcgatgc tccgtcttct
      421 tttttcacct caacggacgg tgcctcttct ttgtcattca ggacaaagaa aataataatg
      481 cctacggcaa tcacaataag ggaatagaac cagatgcctg ctttaaaacc taataagcca
      541 ctgccaaacc aggtaaatac tgccaacgca gaaaatgcca ccacggtatc gacaatgcca
      601 cgccctgttt cgaagaagcc aaacaaccga ccttgttgtt cgctattgcc caatcgactt
      661 accgacttga gcaagaccgg ccagttcatc atgtcgcaag taacgccaaa tagcgcccag
      721 acaaagagga tgccccaata ccccggcatt gtcgtcaaat aaacacccag taatcctgtc
      781 gcaatgagtg agaatgacat ggtgtatctg cgcggtagtt tatcggcaaa ataaatagac
      841 agaaaaaagc ccactgtggt gacaaatgag tttaccgaca tagcattacc aatttgacca
      901 ttggtcaaat ggaaatattc ctgcatcggg atataaaacg catctttcag cgacggtaat
      961 ttataaattg taccgccaca cagtgttaat agactaaata acgcccattt ctttttcgtg
     1021 agcataacat cccctaagcg atttttaaaa tcactgtttt aaaggcaata agataccgac
     1081 cattcatatg gaatggtttt attaactgcg tatgagttaa aacttattag tggttcagaa
     1141 gatggtttgc ctcttgtttg atttgttgta gtacgttacg cacatgattt atctgattaa
     1201 ttgcatcttc tttttcttta cgtaaccgcg catccagtaa atgattttct ggtaggggtg
     1261 tttcactcat ctggcgataa gggatccacg gattatcatc ttcgtcttca aagcggaacg
     1321 ccgacgcata gtaatcaacc tcttcttcca ggccatatgc cgtcacctgc ggctcatcat
     1381 cgccaaggca gataaggtgc gttaataacg ctttgaatgg catatcaccc tgacctgaaa
     1441 tacaggcttt atgacccagg ccaccctgtt ctttaacgat caaggcatct ttaatatgga
     1501 cctgggtaat atgcggtgcc atcgttttta aggcgtcgat gggatgctca tttgcattga
     1561 tcatgttagc aaaatcaaac agtaaggaaa gagactccat ctcactttct ttgaccagcg
     1621 acaccagctc atgacttttt aaatcttcat gctgctcaat cgtaaaagtc aggccgctgt
     1681 cctgatacgt ttcccgtaca taggcaatgt cgttagcgat aatcgataat acgtcgcgca
     1741 aattaccttc ataacatgga taaaaacgta cggacgttgc cccagttttc aacgcgatgg
     1801 cgacggcttc gtcaatagat gccttatctg aagcgctggt ttcaatatga atatcaaggt
     1861 tcagacggcg ggctttatcc ccaaaggcag agagttcttt atcgtccata ttaccaagag
     1921 aaaaacgctc gccatccaga acatggattt tgacgccacg tagattattt tcacttgcga
     1981 tatcaagtaa atcagcaggc aacactcttt ccagccgcat atttaaatga aaggcataag
     2041 catgtaaata aagaggaaga gtgtcgacac gttgtagaat ctttcttgcg ttatttattg
     2101 tcaccataaa acacctacag attaatgatg aggtctacgg cctcaaatcg ccaatattca
     2161 atatctatct ggacaatatt gccttgctgg tcggcgcgat gcttttcaat taagatcgct
     2221 ggcatacccg cagaaccgcc aagatacttg ctgatatcgc ccggcattct gacgggtttg
     2281 aaaatcaatt tttttactac cgtctctttt tggtagcact tttcccagac tgcagaaaat
     2341 gagtggtttt caagttgttc aataaaaccc ggagcaactt caggattaat atatgtttcg
     2401 tgatagaaaa ctttatgtcc ttccagcgcc ccccagccgg tgatgcgata gagttcagtt
     2461 gatggcgtca ctgcaatcag tggcgcgagc gtctcgggaa tatcgctggt acggtttttc
     2521 tcggtaaacc cccaagaagg ctctcgccct tgctcaattg cggcccgctg aaagctcgcc
     2581 gacagctccg gactgtaatt aaatcgcggc tgggtcacga accacccctt acgatcctta
     2641 cggaagattt tggattctgc ctcgaggttc aacaacgcct ggcgcagcgt catacgttta
     2701 atgcccagca gttctcccag ctctctttct gaagggagtt ttccacccgg cgtggtgatg
     2761 ccatctttca accaccgatc cagtttctct tttgcatttt ctacggtgga ttgattctcc
     2821 gccattgttt aatccttttg gtttaaacca gatgaagcat cttacgctta cgccaaaaaa
     2881 gaaagcaatt aacggtgtat gactttgcag ataattgtga caaagctctc tattgacgta
     2941 atcacagact tataagggag atactcgcat tggtgtgcgc tcacgacagg taaaaaaaaa
     3001 cctgccatcg ctggcaggtt ttttatgact aaaaaacgaa attaatcgtc tttcggtgcg
     3061 cggttggcgc ggcgacgatc gttttccgtc aggtgacgtt tacgaatacg gatagaggtc
     3121 ggagtcactt ctaccagttc gtcatcatcg atgaactcca gagcttgttc cagagtcatg
     3181 cggataggcg gaaccagaac aacagcttcg tcagtaccgg aagcacgcat gttggtcagt
     3241 ttcttaccgg tcaggcagtt tacagtcagg tcgttagagc ggctatgaat accgataatc
     3301 tgaccttcgt aaacttctgc accgtgaccg aggaacagct taccgcgatc ctgcagaccg
     3361 aacagcgcga acgcgacagc tttaccctga ccgttagaga tcagtacacc gttctgacgc
     3421 tgacccactt cgcccggacg tacgtcgtcg tagtggctga aggtggagta cagcagacca
     3481 gtaccggaag tcatggtcat gaactcagaa cggaagccaa tcagaccacg gcttgggatc
     3541 acgtagtcga gacgtacgcg gcctttaccg tctggattca tgtttttcag gtcgccttta
     3601 cgttcgccca gcgcctgcat cacagaaccc tgatgctgtt cttcaacgtc cagcgtcacg
     3661 ttttcatacg gctcttgttt acgaccgtct atttcacgga agattacttt cggacgggat
     3721 accgccagtt cgaaaccttc acgacgcatg ttttcgataa gaacagagag gtgcagttca
     3781 ccacgaccag aaacgcggaa cgcatcggcg tcttcggttt cttctacgcg cagcgcaacg
     3841 ttgtgtacca gttctttgtt cagacgatcg aggatctgac gagacgttac gaacttacct
     3901 tctttaccgc agaacggcga ggtgttaacg cagaagaaca tagaaacggt cggctcatca
     3961 acggagagtg ccggcagcgc ttcaacgttt tgcgtgtcgc aaacggtgtc agaaatgttc
     4021 agttcgccca ggcccgtgat cgccacgata tcgccagctt ccgccagatc ggtttcgata
     4081 cgttccagac cgaggtggcc cagcacttta ccgactttcg cgttacgggt tttgccttcg
     4141 ctatcgatga tagtaacctg ctggttcggc ttcactttac cacgcttgat gcggccaatg
     4201 ccgataacgc caacatagct gttgtaatcg agctgagaaa tctgcatctg gaacggaccg
     4261 tcaaggtcaa cgtccggcgc aggaacgtgg tcaacaatcg cctgatacag cggggtcatg
     4321 tcttccgcca tatcttcgtg gtccagaccc gcgataccgt tcagcgcaga agcgtaaacg
     4381 atcgggaagt ccagctgctc gtcggtcgcg tcgaggttaa cgaacagatc gaatacctga
     4441 tccacaaccc aatcaggacg cgcgccaggg cggtcaactt tgttgataac aacaatcggc
     4501 ttcaggccgt aagcaaacgc ttttttggtt acgaagcgcg tttgcggcat cgggccgtca
     4561 aatgcgtcaa ccaccagcag cactgagtct accatggaca ttacacgttc aacttcacca
     4621 ccgaagtcgg cgtgccccgg ggtatcaacg atgttgatac ggtaatcatt ccatttgata
     4681 gcggtgtttt tcgcgaggat ggtaatccca cgctctttct ccaaatcgtt ggagtccatc
     4741 acgcgctctt gggtttcggc acgagagtcg aacgtaccgg attgttggag cagcttgtct
     4801 accagggtgg ttttaccatg gtctacgtgc gcgatgatgg cgatattacg caatttttcg
     4861 atcacaactt tgcctcaggc attagaaata gcgcgttatt gtacacggat taatcgcact
     4921 acaaaacagg atcacaaaca tcctccgcaa acaagtattg cagagtccct ttgtgatcgc
     4981 tttcacggag cataaaaagg gttatccaaa ggtcattgca ccaatatggt gcttaatgtt
     5041 tccattgaag cactatattg gtgcaacatt cacatcatgg tgcagccctt ttgcacgatg
     5101 ttaagcatga taacgccttt taggggcaat ttaaaagttg gcacagattt cgctttatct
     5161 tttttacggc gacacggcca aaataattgc agatttcgtt accacgacga caatgaccaa
     5221 tccaggagag ttaaagtatg tccgctgaac acgtactgac gatgctgaac gagcacgaag
     5281 tgaagtttgt tgatttgcgc ttcaccgata ccaaaggtaa agaacaacac gtcactatcc
     5341 ctgctcatca ggtgaatgct gaattcttcg aagaaggcaa aatgtttgac ggctcctcga
     5401 ttggcggctg gaaaggcatt aacgaatccg acatggtgct gatgccagac gcatccactg
     5461 cagtgattga cccgttcttc gccgactcca ccctgattat ccgttgcgac atccttgaac
     5521 ctggcaccct gcaaggctat gaccgtgacc cgcgctctat tgcgaagcgc gccgaagatt
     5581 acctgcgttc cactggcatt gccgacaccg tactgttcgg gccagaacct gaattcttcc
     5641 tgttcgatga catccgtttc ggatcatcta tctccggttc ccacgttgct atcgacgata
     5701 tcgaaggcgc atggaactcc tccacccaat acgaaggtgg taacaaaggt caccgtccgg
     5761 cagtgaaagg cggttacttc ccggttccgc cggtagactc tgctcaggat attcgttctg
     5821 aaatgtgtct ggtgatggaa cagatgggtc tggtggttga agcccatcac cacgaagtag
     5881 cgactgctgg tcagaacgaa gtggctaccc gcttcaatac catgaccaaa aaagctgacg
     5941 aaattcagat ctacaaatat gtcgtgcata acgtagcgca ccgcttcggt aaaaccgcga
     6001 cctttatgcc aaaaccgatg ttcggtgata acggctccgg tatgcactgc cacatgtctc
     6061 tgtctaaaaa cggcgttaac ctgttcgcag gcgacaaata cgcaggtctg tctgagcagg
     6121 cgctgtacta cattggcggc gtaatcaaac acgctaaagc gattaacgcc ctggcaaacc
     6181 cgaccaccaa ctcttataag cgtctggtcc cgggctacga agcaccggta atgctggctt
     6241 actctgcgcg taaccgttct gcgtctatcc gtattccggt ggtttcttct ccgaaagcac
     6301 gtcgtatcga agtacgtttc ccagatccgg cagctaaccc gtacctgtgc tttgctgccc
     6361 tgctgatggc cggtcttgat ggtatcaaga acaagatcca tccgggcgaa gccatggaca
     6421 aaaacctgta tgacctgccg ccagaagaag cgaaagagat cccacaggtt gcaggctctc
     6481 tggaagaagc actgaacgaa ctggatctgg accgcgagtt cctgaaagcc ggtggcgtgt
     6541 tcacggatga agcgatcgat gcgtacatcg ctctgcgtcg cgaagaagat gaccgcgtgc
     6601 gtatgactcc gcatccggta gagtttgagc tgtactacag cgtctaatag ttgaagttgt
     6661 actacccggc gcacaacgcc gggatttagt tgctgtggaa actttcagcc catctctgca
     6721 tgggcttttt tctccgtcaa ttctctgatg cttcgcgctt tttatccgta aaaagctata
     6781 atgcactaaa atggtgcaac ctgttcagga gactgcttta tggcaacagg cacgcagccc
     6841 gatgctgggc agatcctcaa ctcgctgatt aacagtattt tgttaattga tgacaatctg
     6901 gcgatccatt acgccaaccc cgccgcgcaa caactgctcg cccaaagctc ccgcaaattg
     6961 tttggtacgc cgttaccgga actgttgagc tacttctcat taaatatcga gctgatgcaa
     7021 gaaagtctgg aggcggggca aggctttacc gataacgaag tgacgctggt catcgatggt
     7081 cgctcgcata tcctttctgt gacggcccag cgtatgccgg acggcatgat cctgctggag
     7141 atggcaccga tggataacca gcgccgctta agccaggaac aactacaaca tgctcaacaa
     7201 gtagctgccc gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc gcttggcggt
     7261 ttacgtggcg cggcgcagtt actcagcaaa gcgttacctg acccatcact actcgaatat
     7321 accaaagtga ttatcgaaca ggcggaccgg ctgcgaaatt tggtcgaccg tctgttgggg
     7381 ccgcagctgc ccggtacgcg cgttaccgaa agtattcaca aagtggctga acgcgtggta
     7441 acgctggtgt cgatggaact gccggacaac gtgcggttga ttcgtgatta cgatcccagc
     7501 ctgccggaac tggcgcacga cccggatcaa attgaacagg tcttgctgaa tattgtgcgc
     7561 aatgcgctac aggcgctggg gccggaaggc ggtgaaatca ttctgcgtac ccgcaccgcg
     7621 tttcaactga ccttacacgg cgagcgctac cggctggcgg cgcggattga tgtggaagat
     7681 aacgggccgg gcattccgcc tcatttgcag gatacgctgt tttacccgat ggtcagcggc
     7741 cgcgaaggtg gcaccgggct tggcttatcc atcgcccgta atttgattga tcagcattca
     7801 ggcaaaattg aatttaccag ttggccaggt cataccgagt tctcggttta cctgcctatc
     7861 aggaaataaa ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat gacgatagtt
     7921 ccatccgttg ggtgcttgaa cgtgcgctcg ctggagcggg tttaacctgt acgacatttg
     7981 agaacggcgc ggaagtactg gaggcgctgg cgagcaaaac gccggatgtg ctgctttcag
     8041 atatccgtat gccgggaatg gacgggctgg cgctgctcaa gcagattaaa cagcgccatc
     8101 caatgcttcc ggtcatcatt atgaccgcac attccgatct ggatgctgcc gtcagcgcct
     8161 atcaacaagg ggcgtttgat tatctgccca aaccgtttga tatcgacgaa gcagtggcgc
     8221 tggttgagcg cgctatcagt cattaccagg aacagcagca gccgcgtaat attcagctta
     8281 acggcccaac gaccgatatc atcggcgaag cgcaagccat gcaggacgtg ttccgtatta
     8341 tcggtcggct ttcgcgttct tctattagcg tgctgattaa cggcgaatcc ggcaccggta
     8401 aagaactggt cgctcatgcc ctgcatcgcc acagtccgcg caccaaagcg ccgtttatcg
     8461 cgctgaatat ggcagctatc ccaaaagatt tgatcgaatc agaactgttt ggccacgaga
     8521 aaggcgcgtt tactggcgcg aataccattc gtcaggggcg ttttgaacag gccgatggcg
     8581 gcacgttatt tctcgacgaa attggtgata tgccgctgga tgtgcagacg cgtttgctgc
     8641 gcgtgctggc agacggtcag ttttatcgcg ttggcggcta tgcgccggtg aaagtggatg
     8701 tgcggattat cgctgccact caccagaatc ttgaacagcg ggtgcaggaa ggtaagttcc
     8761 gtgaggatct gttccaccgc ctgaacgtta tccgcgttca tctgccgccg ctgcgcgaac
     8821 gtcgggaaga tattccccgt ctggcgcgcc attttttaca ggttgccgcg cgcgaactgg
     8881 gcgtagaagc gaagttgctg catccggaaa ccgaagctgc tctgacgcgt ctggcgtggc
     8941 caggcaacgt gcgccagctg gaaaacacct gccgctggct aacggtgatg gccgccgggc
     9001 aggaagtgtt gattcaggat ttgcccggcg aactgtttga atcaacggtt gcggagagta
     9061 cttcgcaaat gcaaccggac agttgggcga cacttttagc gcagtgggca gacagagcgc
     9121 tgcgttccgg tcatcaaaat ctgctttccg aagcgcagcc agagctggag cggacgttac
     9181 tgacgaccgc gttgcgacat acgcaggggc ataaacagga agcggcgcgg ctactcggct
     9241 ggggccgcaa cactctgacg cgtaagttaa aagagctggg gatggagtga ttcacggctt
     9301 gtgtgtaaag attgattatt gagcgcaaat tgctggtatt ttgcgcttta ctgttccgat
     9361 aagttcagta tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa tcaataatta
     9421 atctggtatc gagtggcgca gttgacagcc acacaccgca aactgccgtt gctgccgtgc
     9481 tgtgtgccgc gatgattggg ctgtttagct gaagctcgtt gtttatgccg gatgcggaga
     9541 aagtcgtagg cctgataaga cgcgaccagc gtcgcatcag gcatcatgct cagatgcctg
     9601 atgcggcgta aacgccttat ccggcctaca aaagcaataa tttcaacagg ttttgttgcc
     9661 cagcgcatca ggcaatcttg ttctcagcac attgatggcg ctttcgtttt ctactttgta
     9721 aacgaagcgc cattcactct ttaaatcacc cgtgagaact gctgcatccg cgctttctgg
     9781 cgcagatagg tatcaaagca catgcaaatg ttgcggatca gcaagcgacc tttcgccgtc
     9841 acctgtattc ccttctcatc cacatccacc agcccatctt ttgctaacgg ggcgagcagc
     9901 ttgagatctt ccgcaaagta atcagcgaag tgcaaatccc actgtttctc aagaggggcg
     9961 taatccagac ggaagttgca gatgagcgac ttaatcacat cgcggcgaat acagtcatca
    10021 cgcgttagcg caataccacg ccacagcgca ttgccttgtt catacacttg ctgatagtac
    10081 tgcttcaact ctttctggtt ctgcgcgtag cagtcgccaa tcatgctgat ggcggaaacg
    10141 cccatcccca gcagatcggt atcgccctga gtggtgtagc cctggaagtt acgatgcagc
    10201 acgccttcac gctgggccac cgccagctcg tcatcaggac gggcaaagtg atccataccg
    10261 ataaactgat agcccgattg cgtcaggaag gcgatggttt cctgcaggat atcgagtttt
    10321 tgctgcggac tcggcaggtc agcatctttg attttgcgct gagcagcaaa aatggtcggc
    10381 agatgcgcgt agttaaaaac gctcagacga tcggggttca gctccgccac acgtttcagg
    10441 gtaaaggcga aactctccgg cgtctgtttc ggcaggccgt aaatcaggtc gatgttggtg
    10501 gaggtaaaac caatctcacg cgcatggtta agcagtgcaa agatgaactc ttcatcctgc
    10561 tcgcggttaa ccagacgttg cacttctttg ttgaagtcct gcacgcccat gctcaggcga
    10621 ttaaagcctt cggcgcgtaa atgatcgagt acatccagtt cgatttcccg cggatcgact
    10681 tcgatcgaaa tctccgcatc ggtattgaaa tggaagtttt cgcgcagcag cttcatcaga
    10741 cggctgattt gcgctttatt cagatacgtc ggcgttccgc cgccccagtg caattggctg
    10801 acgtgacgcc cggcgaacag cggtgcacga tggacgattt cttgctccag cgcgtccaga
    10861 tactgatcgg ccttgtgctg ctggcgagta acaatcttat tgcaaccgca gaagtaacaa
    10921 agcttatggc agaacgggat atgtacgtag agagataatg gacgctcagg atagcgcgcc
    10981 acggcttgta aaaacgcctg ttcgccgaag tcttctgaaa actccagcgc ggtcgggtac
    11041 gaggtgtatc gtggcccgga atagttatat ttctggatca gggccagatc ccagtcgatt
    11101 tgctgtacag acatgctcac tccttccgat ggcgtctctg gcggctacgg cgtaccggcg
    11161 gatgcccgtt tcgggtcatc agccggttgc gcagccactt ttgtcgccgc gacaaccgct
    11221 gtagtttaac gaataaccac accagataac atataaccgg aagggttatc agcagaacgg
    11281 gaaggcccac ggcggcaatc cgttagttgc cccgcagcag acgcatcatg tcttcttgct
    11341 tctcgtcttc ttcctcttct tcgtcatcgt cataagagag gccgagtttc tgcatcaact
    11401 catcaatacg atccagttta gcatccaccc aggattgctc ttcggcgctc agggtttcgc
    11461 ctgcttccag acgttccagc agcgcatcca gacgctcatc cgtttccagt aattccaact
    11521 ccgcctgcgg tgaaagcata ggtttctcac tcttcggttt gtgctgtttg gtgacttttt
    11581 cagtcactcc caatggaata ggggttttac tgccaatacg tggatctttt ggtgcgttct
    11641 ggcctttgct gcctgacgtg gtgtttccgc ccgctgcgcg gctgcccggc gcatgaccac
    11701 gacgtttttt ctgacgctta cggtcacgag cttcctgatc cagctcctcg cgtgtttttc
    11761 gacgcgcttt tgcatgacct ttgctgcgtg agtttgaaga tgatggtttc ataatggttc
    11821 gtcttttagc cgttttattc agtatagatt tgcggcggaa tctaacagaa agcaagcaaa
    11881 gaaaaaaggc gacagattac tctgtcgcct tttttcctga ctcataaccc ttaacgggtc
    11941 ttacaatcct tgcaggccgc caacacaccc tgtttttcca ttagccggaa caatccgtgt
    12001 tgattccatt tccgtttaat tacgtctccg gacgtttgtc ttcctgacaa tcctctgtct
    12061 tcgcctcctg gcgctcctga ccctcatcct gagtcattgt tcctgttagc gtcctcgctc
    12121 tatgggtgct actttacgcc tttgctttaa acaaacaatc aaccgccatc agtatttgca
    12181 cattttcaga gttttatata aaagcaattc actgatttat atagcttatt tgacatgcat
    12241 cgcagaattt tctctgctat ttcgcataaa gactatcggc aatgtctcac aaagcgtatg
    12301 gcttttactt acaacgctca cgccgaaaaa tccgcctttt attatggttc aggtataatc
    12361 gccacaaagc tttgactgac ggagacgacc gctttgacta atttgaatta tcaacagacg
    12421 cattttgtga tgagtgcgcc tgatattcgc cacctacctt ccgataccgg aattgaagtg
    12481 gcttttgcag gccgttccaa cgcaggtaaa tccagcgcgc tgaacacgct gactaaccag
    12541 aaaagcctgg ctcgtacctc aaaaacccca gggcgcaccc agcttatcaa cctgtttgaa
    12601 gtggctgacg gcaagcgtct ggttgacttg cctgggtacg gttatgcgga agtcccggaa
    12661 gagatgaagc gcaaatggca gcgtgcgctc ggcgaatacc tcgaaaaacg tcagagcctg
    12721 caaggtctgg tggtgctaat ggatattcgc catccgctga aagatttgga tcagcagatg
    12781 attgagtggg cggtagacag caatatcgcc gttctggtgc tgctgaccaa agcggacaaa
    12841 ctggcaagcg gcgcacgtaa agcgctattg aatatggtgc gtgaagctgt actggcgttt
    12901 aacggtgatg tgcaggttga aacgttttct tcgttgaaga aacaaggcgt agacaagctg
    12961 aggcagaaac tggatacctg gtttagcgag atgcagcctg tagaagaaac gcaggacggc
    13021 gaataatttt cttgccttaa tgcttgtgcc ggatgtggcg tatccggccc gtaaattcaa
    13081 tagattacaa ttctaccgtt ggcctgataa acatagcgca tcaggcatta cggatctttt
    13141 ctttcgccca ataaaaaacg ccccagtcat tactgactgg ggcggctaaa atattcagcc
    13201 aaatccgatt acgtgaagta aaaggtctga aagatagaac atcttacctc tgtaccctac
    13261 gcgactaact ttactctttt ttgttcagtt cagaaagcac tttttgtaat tttttttcag
    13321 ttgttgcata ggaatttcta atagccatca caaaacgtga cagcttatgt tgcttactta
    13381 cgaaaaaagg gcatgttcag gcgaatctta gtgcgcctga tcccagtttt cgccactccc
    13441 cacttccacc agcaacggca catccagacg ggtacagttt tccatcagtt gatgaatctg
    13501 cttcgcgacg gcatcaacat catctttatg aacttcaaat accagttcat cgtgtacctg
    13561 catgatcata cgtacacgcg gttgctcagc ctgtaaccac gcatcaacgg caatcatcgc
    13621 ccgtttgata atgtcggcgg cggttccctg cattggcgcg ttaatggctg cacgttcagc
    13681 cgctgcacga cgagcaccat tgctggattt gatatccggc agatacagac ggcgtccgtc
    13741 cagcgtttca acgtagccca gctctttcgc ctgagcacgg gtgcgttcca tatactccag
    13801 cacgccaggg tagcgttcga agtaaaggtc catgtacttc tgcgcttctt tacgtggaat
    13861 gttcaattgc cgcgccagac cgaaagcact catgccataa atcagaccaa agttgatcgc
    13921 tttcgcgcta cggcgttgct cgctggtgac ggtttccagt ggcaaaccaa acacttctgc
    13981 cgccgttgcc cggtggatat cttttccttc cgcgaatgcg gtcagcaagc ctttatcacg
    14041 cgagagatgc gccataatgc gcagttcaat ctgcgagtag tccgctgaga caatcacata
    14101 atcctctggc gcaataaacg cctggcggat acgacgacct tcttcgttac gcaccggaat
    14161 gttttgcagg ttaggatcgg ttgacgataa acgtcccgtt gcagttactg cctggtgata
    14221 agaggtatgc acacgcccgg ttttcgggtt gatcatcagc ggcagcttgt cggtgtaagt
    14281 cgacttcagc ttcgccagac cacgatactc cagaatcact tttggcaacg gatagtccag
    14341 cgccagttct tccagtacct cttccgacgt tgacggcgcg ccacccggcg ttttcttcag
    14401 cggtttaata ccctgttttt caaacagaat ggtttgcaac tgcttggtgg aagaaaggtt
    14461 aaattcttca cctgcaattt catgcgcttt cttttccagc tcagccagac gcaaggtgag
    14521 ctcttcagaa tgattgtgca gcactttcgg atcgatcttc acaccgttac gttcaatgcg
    14581 tgaaagcacc ggcaccagcg gcatttcgat attctcgaag acgttcaacg gccctttgtg
    14641 tttctgcaga tccggccaca ttttcagatg caactgcaag gtgacatctg catcttcggc
    14701 ggcgtaacgt ccggcctctt cgagggcaat ctggttaaag gtcagttgat ttttgccttt
    14761 acccgcaatc tcttcaaaag tgatggtttt gtgcttcaac caacgttccg cgaggctgtc
    14821 catatcgtga cgcccggcaa cgctattgag aatgtaggac tccagcatgg tatcaaacgc
    14881 aatcccacgc agttcaatgc cgtaattcgc cagaataccg cgatcgtatt tcaggttttg
    14941 cccgaccttc agcgcctttt catcttccag cagcggtttt agcaactcga gtgcacgctc
    15001 gtgagagatt tgatcgggcg catcaagata atcatgagca accggaatat atgccgctac
    15061 gcctggctcg atagcaaaag aaagcccgac caggttagca gagatgttat caaggctgtc
    15121 ggtttcggta tcaaatgcaa ataccggcgc tttttccagc ttcgcaatcc acgctttcag
    15181 tgtttcttca tcaaggatag tgacgtagtt gtcataagaa atcaccgttg ccgtcacttc
    15241 tggcgcttcg tctgcaacac tggtttcctg cggcttcgcg gctgtttttg cccctttggc
    15301 ctgtaaccat ttgcccactt cgacatcagc agtccagcgt ttgaactcat actttttgaa
    15361 cagccccaac aactcttccg ctgccggttg ctgcacttcc agttgttcac aggtcagctc
    15421 cagttcaaca tcggttttaa tcgtcgccaa ctggtatgag agataagcaa cttctttgtt
    15481 ttgctcgagc ttcgctgcca ttgttttcgc gccacggaag ctcaacccag caattttttc
    15541 tggctcggca tacagcgtat ccagcccgcc aagaccttgc agcaatgcct gcgcggtttt
    15601 ttcaccgacg cccggtacgc caggaatgtt atcagaggag tcacccatca gcgccaggaa
    15661 gtcgatgatc aattctggcg gcacgccgta cttattcacc acctcttccg gtccgaggat
    15721 ggtattcgtc atggtattga taagcgtaat atttggcgtc accagctgcg ccatatcttt
    15781 atcgccagtg ctaatcagca ccggacgccc ggctttttcg gcttcgcgcg ccagagtacc
    15841 gataacgtcg tccgcttcta cgccagaaac cgccagcagc ggcagtccca tcgctttaac
    15901 catcgcgtgc agaggttcga tttgtgctcg cagatcgtcc ggcattggcg ggcgatgtga
    15961 tttgtaatgt tcaaacagtt catcacgaaa ggtttttccc ttggcgtcaa agaccaccgc
    16021 tgcatgcgtc ggtttatatt gcatgatcag actgcgcagc atgttgagga caccatacat
    16081 cgcaccggtc ggctcgcctg cgctgttagt cagcggggga aacgcgtgat atgcgcgata
    16141 aagataagat gaaccatcta caaggataag tggattttgg gggatctgaa ccataatgtc
    16201 cgtgcctgtt tatcagatta tgggtaaagg atgccacaga caggatgaaa acatgagttt
    16261 ttcaccgctt ttcgtgaaaa tcttttgtag atctgctgga tcgctcgcaa atcgtcatgt
    16321 ggataacttt gtgcataaat tatttacatc gcttttaatt tattatcatg ctttttacag
    16381 catgatgttt gtctttgttt ttcatgcagt taaaaataaa tccatcccta ataattgaga
    16441 taatgggatt ttttctaatg tggatagata tgaattattt ttctccttaa ggatcatccg
    16501 ttatttgggt cattttctgc taaaatctgc gccttgcgct tctttagtgc actgtctatg
    16561 gctaaccttt tgaataaatt cattatgacg agaatactcg ctgcgataac ccttttgctg
    16621 agtatcgtat tgaccatttt ggtcactatt ttttgttccg tcccgatcat cattgccggg
    16681 attgtaaaac tattgctacc tgtgccagtc atttggcgaa aggtttcgcg tttttgtgat
    16741 tttatgatgt attgctggtg tgaaggtctg gcggtattac tgcatctgaa cccacacctg
    16801 caatgggaag ttcacgggct ggaagggtta agtaagaaga actggtatct gcttatttgt
    16861 aaccaccgta gttgggcaga tattgtcgta ctgtgcgtgc tgtttcgtaa gcacattccg
    16921 atgaataaat atttcctcaa gcaacagctg gcctgggtgc catttcttgg cctggcgtgc
    16981 tgggcgctgg atatgccgtt tatgaagcgt tattcccgcg cttatttatt acgtcatccg
    17041 gagcgacgtg gtcaagatgt tgaaaccact cggcgttctt gtgaaaagtt tcgcctgcat
    17101 cccaccacca ttgtcaattt cgttgaaggc tcccgtttca cgcaagaaaa acatcagcaa
    17161 acccactcca cttttcaaaa cttgttgcca ccaaaggcgg caggcattgc gatggcgcta
    17221 aatgtactgg gtaaacaatt cgataaactg cttaatgtta cgctgtgtta tccggacaat
    17281 aaccgtcagc cgttcttcga tatgttaagc ggtaaactga cgcggattgt cgtacatgtg
    17341 gatttacagc ctattgccga tgagttacat ggcgactaca tcaatgacaa aagctttaag
    17401 cgccattttc agcaatggct gaactcattg tggcaggaaa aagaccggct tctgacgtct
    17461 ctcatgtcat cacagcgtca gaataagtaa gttttactcg cctctgaatg agcagaggcg
    17521 agtgagtatt ttaatgaaca aaacgtccgg cacgagacat aaattcttct tcgctcatct
    17581 cctgtccgtt aaaaacaact ttacccggtg tataacgcag ctgcagcgag gcggtattgt
    17641 tgtcaatcgt gattaactga aacatctgac ctaatgtcat catcccgctg atttgtttat
    17701 cagcctgctt ttgcgctttt tccgcatcca ttccttcaga taaattaagc tgttttgcgg
    17761 tttctgtgac gacattaagc ggtaacttca catcaaaatt gagcgatttg atatctttgt
    17821 ttgtggatga tgaagatttg gctgggtcgg caatactgat atccagattg gcatttagtt
    17881 cgccgagtgc gttcttccat cttaccggtt gtttaatggt cggctcactt ttttgtaata
    17941 acggtaaata ctctttgaac aatgcagcat tcacttcttg cagagcaact tcatcgtttg
    18001 ctaactcagg gtgtgcagca agctgcttct gcatcgcaat gttatactgg atgataaatt
    18061 ggcgcactgc tgagggatca atagattcag caatcaatga ccactcacca ctaccgagtt
    18121 gctgattccc ttttgttaac ttatcaagtt cgtaggtcag attgacattg atataatctt
    18181 tgtctttaac gcgatccagc ctggtttttg caacgaaagc atcgatttgg gcaacgtctt
    18241 ccccgtgatt gataatgtta atctgattaa atttcgactc gctttcacca accggaaatt
    18301 tcttttcttc taatcgcgcc agagaatcaa tggtaaaact ttttgccgtt actttgttca
    18361 ttggtgataa ttgcagaaca atatcttcga ctttgccttc aatatccagg ttagacaatg
    18421 aactaccatc accattaaaa gtaaactggc cctcaccaaa agaaaatttt ccatactctg
    18481 caggggcaac atctaatgtc gtagagaatg agccaccaaa cgcgaatcgc aatgtcgctt
    18541 ccacgggcga tttatttttc gcggcgataa atagcggttg tgtcagttca ttattcacta
    18601 agttcacttt tgccgctgcc agtgctggga tgacattacc atgcattaac attgtgatgg
    18661 gcaacggacc gtgctcaaca tccacatcaa atacaacttt ctggcctggc ttgatattga
    18721 gatcgggtgc accgttatcg aatgtcattt gcatttgaaa acgggaatta aaaaagcctt
    18781 tgtcaaagtt tttataactt aacgtcatat cgtaggcatg ttcacctttc tttttcgcat
    18841 cgttaaaatc tttaataaac ttttcgatac caggctgaat ttgcgtacct gtgtaccatg
    18901 tgccaccacc ccagattacg gctaacgcaa caataacacc tgtagctgac ttacgtatca
    18961 tataaatatt ccctgataaa acagcaaaac tttgaatatc cacttatgct gaaataatgt
    19021 ccagcggcag gatgcattat cagaatgtat ttatatttat taaataataa aaaaagcccg
    19081 tgaatattca cgggctttat gtaatttaca ttgaattatt tttcctcgga cagatatttc
    19141 accgtatcag catactgctg aacaaaaaca tccatattgc tggtatccat accctgcgga
    19201 ttcagctgat atttaccgtt aacaaacatc gccggaacac cacgcaattg cacatcagct
    19261 gcggcttttt cctgctgagc gaccagagat ttcaccacga agctgttcca cgccgcgtcg
    19321 tactcttcac ctttaatacc tgcattgata aatacatcgc ggatatcaga tgctgaacga
    19381 atggtctggg ttttctgtac gccttcaaac agcggaacag tcactttgtc ttccacgccc
    19441 agcgccatcg ccacagccca tgcctgagtc agatctttgc ccaggtcacc acccatgaag
    19501 ttgacgtggt atttagtcat cttcacgcct tccggcagtt ttttcttcac attatcagaa
    19561 atatgcagaa cttcttcaaa ctgatagcag tgcgggcaga agaaagagaa aaactccagc
    19621 acttgcggcg cgccagctac cggtttttcc agggtagtgt actgtttacc atcttcatac
    19681 tgcgccgccg atgcgctaaa cgctaaaact aaaccagcca gcgccagcca aatctttttc
    19741 atgatctact ctctccgatt aatacatagg tgttaattgc aaagggggtt cttgtagaac
    19801 ttttgcctgt tctataaaag tcgccgtctg tcgtagccag taatcttccc cggttaacca
    19861 cgggaaattt ttcgggaacg cgggatcagc ccaatgccgc attagccagg caagataata
    19921 aaccaaacgc atggcgcgta aaggttcaat cagtccgatt tcagcggtgt cgaactcgct
    19981 aaattcttca taagcttcaa taatagtttc cagttgcatc cgctgctggg ctttatcgcc
    20041 attgagcaac atccacaaat cctgaatagc tggaccatta cgtgcatcat ccagatcaac
    20101 aaacattgga ccatcgcgcc agagaatatt cccggcgtgg cagtctccat gtagccgcag
    20161 aacggtgaaa tcttcccgcc agtgtgctgt aacggcggcg atcagctcat ctgtcgcttt
    20221 cagaaatgcc gctttcaacc cggaaggtat cagtgtagcg tcctcaaaca gcttgcgagg
    20281 ctcaatgaga tattcattca gaccgatggt cgggcgatga ataaaaagct gtttgcgccc
    20341 cgtctggtgc atacgcccta aataacgccc aaccgcttcc atctgatcga tattatcagc
    20401 ttcgaactgg cgaccaccga cgcttggaaa aacagcgaaa taaaatccct gatgattcaa
    20461 taaagtctga ccgttaaagg ccacaggtgc tgcgaccgga acttcatcat ttaccagctg
    20521 caacgcaaat tgatgttctt cgaggatttg atcggctgtc caacgttcag ggcgataaaa
    20581 tttgacgaca aaacgtcgac gctcttcgtc ctgaaattga tagacacggt tttcatagct
    20641 gttaagcggg gtaagaccgg aatccacccg gatcccttgc ttaaacagag cgtccatgat
    20701 ggtatccggg tgtagtgtct ggaaagtaaa agcgctgttg ttcatcccat catccggaaa
    20761 acattacgaa tgatgaagga tatcatttca cgatgatttt ggtggcgccg cttacaagct
    20821 tttactcttt aattacgccg cgagcgcgta acagcgccgt tttgaagtct tcttcataat
    20881 ctttctgcaa acccgggatc accgcatctt tcgcggaatc acgcattttc aggtgataga
    20941 tcagaatgtc atccgtcaaa tccgccagtt cgccgtcaaa acctgactct ttcgccagtt
    21001 tctgcaaaaa ttgcaacaag ttaaagtctg gctctttttg ccaggctggc tggaggagtt
    21061 caataacttc attcagacgt ttacatttca tagtaatgct ccttactctt gagacagaca
    21121 cgttagcagg gtcaatccca caataaaaga ggcgatatcg gtgaatctaa tgacgacgat
    21181 aacaggcgtt gtgctggcag gcggtaaagc cagacgaatg ggcggcgtag ataaaggatt
    21241 gctggactta aatggcaaac cgctgtggca acatgtcgct gacgcgctta agacgcagct
    21301 ctctcacgtc gtggttaatg ctaatcgtca tcaggaaatc tatcaggcag gcggtctgaa
    21361 agtgattgaa gattcactgg cggattaccc aggccctctg gcaggaatgc tttcagtaat
    21421 gcagcaggaa gctggtgagt ggtttttgtt ttgtccgtgc gatacgcctt acattcccca
    21481 tgatttagcc gcccggctta atcatcagcg caaagatgcg cctgtcgtgt gggtccatga
    21541 cggtgaacgc gatcacccga ctattgctct ggtaaaccgc gctattgagc ctttattact
    21601 ggaatatctg caagcaggag aacgccgggt aatggcattt atgcgtctgg ctggcggtca
    21661 tgcggttgat ttcagcgatc ataaagatgc atttgttaac gtgaatacgc cagaggagct
    21721 tgcccgatgg caggaaaaac gatgataccg ttactcgcct ttgccgcgtg gagtggtacc
    21781 ggaaaaacta cgcttttgaa aaaactgatc ccggcattat gcgccagagg gatccgtccg
    21841 gggctgatta agcatacgca ccatgatatg gatgttgata agccaggcaa agatagctat
    21901 gagctgcgca aggctggcgc ggtacaaacc atcgttgcca gccagcaacg atgggccttg
    21961 atgacggaaa caccagacga agaagagctg gatctacatt ttctcgcaag tcgaatggat
    22021 acctcaaagc tggatttgat tctggtcgaa gggtttaagc atgaagagat cgcaaagatt
    22081 gtgctgtttc gcgatggagc cggacatcga cctgaagaat tagtgataga caggcatgtt
    22141 attgctgtag ccagtgatgt gccgcttaat cttgatgtcg cattactgga tattaatgat
    22201 gttgaggggc tggctgattt tgtggtggag tggatgcaaa agcagaacgg ataagcaaga
    22261 gtaggctgga tcagacgcag ccagcgtcgc acccggcatt t
//
LOCUS       DAJTSA010000063        22203 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000063 DAJTSA010000000
VERSION     DAJTSA010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22203
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.063"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="OO369_002583"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="OO369_002583"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR5982814.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="OO369_002584"
     CDS             complement(1672..2730)
                     /locus_tag="OO369_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5982815.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="OO369_002585"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="OO369_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="OO369_002586"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="OO369_002586"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR5982816.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO369_002587"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO369_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR5982817.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO369_002588"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO369_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR5982818.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="OO369_002589"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="OO369_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR5982819.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="OO369_002590"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="OO369_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR5982820.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO369_002591"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO369_002591"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR5982821.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO369_002592"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO369_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR5982822.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO369_002593"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO369_002593"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR5982823.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO369_002594"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO369_002594"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR5982824.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="OO369_002595"
     CDS             complement(17699..18322)
                     /locus_tag="OO369_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR5982825.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="OO369_002596"
     CDS             18619..18894
                     /locus_tag="OO369_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5982826.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="OO369_002597"
     CDS             complement(18971..19213)
                     /locus_tag="OO369_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR5982827.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="OO369_002598"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="OO369_002598"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR5982828.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="OO369_002599"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="OO369_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR5982829.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..>22203)
                     /gene="alaC"
                     /locus_tag="OO369_002600"
     CDS             complement(20977..>22203)
                     /gene="alaC"
                     /locus_tag="OO369_002600"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR5982830.1"
                     /translation="RPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMGNPDG
                     ATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAIVTIG
                     SKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNELERA
                     IRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVYDGWK
                     APSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYGTFTP
                     LQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKIPEPY
                     AAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKAMFRA
                     DGLLPVSSKHIHENAE"
BASE COUNT         6280 a         5162 c         4830 g         5931 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gctaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaaact cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacaactta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caagattaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgat tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aagcgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgataatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattaattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatgattt ttatagcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg acgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgtggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    21301 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccgaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag catttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcg
//
LOCUS       DAJTSA010000064        21512 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000064 DAJTSA010000000
VERSION     DAJTSA010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.064"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OO369_002601"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OO369_002601"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR5982831.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="OO369_002602"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="OO369_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR5982832.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="OO369_002603"
     CDS             complement(2649..3200)
                     /locus_tag="OO369_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR5982833.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="OO369_002604"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="OO369_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR5982834.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5211
                     /gene="cybC"
                     /locus_tag="OO369_002605"
                     /pseudo
     CDS             4829..5211
                     /gene="cybC"
                     /locus_tag="OO369_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="OO369_002606"
     CDS             complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="OO369_002606"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR5982835.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="OO369_002607"
     CDS             complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="OO369_002607"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR5982836.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8410..10065)
                     /gene="treC"
                     /locus_tag="OO369_002608"
     CDS             complement(8410..10065)
                     /gene="treC"
                     /locus_tag="OO369_002608"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR5982837.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10115..11536)
                     /gene="treB"
                     /locus_tag="OO369_002609"
     CDS             complement(10115..11536)
                     /gene="treB"
                     /locus_tag="OO369_002609"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR5982838.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11655..12602)
                     /gene="treR"
                     /locus_tag="OO369_002610"
     CDS             complement(11655..12602)
                     /gene="treR"
                     /locus_tag="OO369_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR5982839.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12787..12840
                     /gene="mgtL"
                     /locus_tag="OO369_002611"
     CDS             12787..12840
                     /gene="mgtL"
                     /locus_tag="OO369_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR5982840.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12981..15677
                     /gene="mgtA"
                     /locus_tag="OO369_002612"
     CDS             12981..15677
                     /gene="mgtA"
                     /locus_tag="OO369_002612"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR5982841.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="OO369_002613"
     CDS             complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="OO369_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR5982842.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="OO369_002614"
     CDS             complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="OO369_002614"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR5982843.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="OO369_002615"
     CDS             complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="OO369_002615"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR5982844.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="OO369_002616"
     CDS             complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="OO369_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR5982845.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18170..18565)
                     /locus_tag="OO369_002617"
     CDS             complement(18170..18565)
                     /locus_tag="OO369_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5982846.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="OO369_002618"
     CDS             complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="OO369_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5982847.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19481..20074
                     /locus_tag="OO369_002619"
     CDS             19481..20074
                     /locus_tag="OO369_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5982848.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20219..20671
                     /gene="tabA"
                     /locus_tag="OO369_002620"
     CDS             20219..20671
                     /gene="tabA"
                     /locus_tag="OO369_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR5982849.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20794..>21512
                     /locus_tag="OO369_002621"
     CDS             20794..>21512
                     /locus_tag="OO369_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5982850.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEKAL"
BASE COUNT         5196 a         5324 c         5668 g         5324 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgctgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaaaagaagc gcaggctgct
     5161 gcagagcaac tgaaaacgac ccgcaacgcc tatcaccaga agtatcgtta attcctcatt
     5221 tccctgttgc ctgcactcag gtaacaggga acttatcatc gcaaatgatg caccacctga
     5281 ttgctgctgc cgcgccagat cagggaaggg tcttttaaat cctgcacaaa tttgccgtcg
     5341 accagcacgt taatcaaatc aacaacctgc atttgcgcag cgttgagttc gtcgagttta
     5401 tagcctgtcc acacccagat gtctttaccc ggacactcgg cgcggatgcg ttttaccagt
     5461 ttcagaatat ccggcacgtt ttgcggatgc agcggatcgc cgccggagag ggaaatcccc
     5521 tggcgtttga tacgagtgtc attcagatct ttaatgatct tgtcttccat tgctttggta
     5581 aatggctgac cagaatttac ccgccatgtg cttttgttat agcaaccggg gcattcatga
     5641 acacacccgg agacaaacag ggtgcagcga gtgccggggc cgttgacgat gtcgacagga
     5701 tagtactgat gataattcat tatttcgcct ggcttatcag tccgcgtgta ggctgcatcc
     5761 ggtaatcaat gcctgatgcg acgctgtcgc gtcttatcag gcctacagcc gttgccaaat
     5821 gtaggccgga taaggcgttt acgccgcatc cggcatttgc ttagcgcaga gtgaagatta
     5881 acctatctgc ccattcccca aatgtttaac gcggcgctta acttcttcct gcttaccagc
     5941 gttaaacgga cgtgcatccg ggctacctaa atatccgcac acgcggcgag ttaccgacac
     6001 acgggaggcg tcatggttac cacatttcgg gcaagtgaag cctttgctgg tgcactcgaa
     6061 ctcaccggta aagccacact cgtagcactc atcaatcggt gtattggtgc cgtaatacgg
     6121 tacatgctga tagctgtaat cccagacatc ttccagcgct ttcaggttgt gctgaatgtt
     6181 tggatactcg ccgtagcaaa tgaaaccacc gttcgccagc ggcgggtaag gcgcttcaaa
     6241 gtcgatcttg tcgtacgggt tcaccttctt ctccacatca aggtggaaac tgttggtgta
     6301 gtaaccttta tcggtcacgc ccggcaccac gccaaactca gcagtatcga gacggcagaa
     6361 gcggtcgcac aggttttcac tcggcgtgct gtaaagactg aaaccataac ccgtttcttc
     6421 tttccactga tccactgcct gacgcagacg ttcaacaatg gcgatacctt tcgcgcgaag
     6481 cttctcgttg tcgtaaacat gctcgccgct gaacagcgcg ttaatggttt cgtggatgcc
     6541 gatgtaaccc agcgaaatag acgcacgacc gtttttgaag atttcagaaa catcatcgtc
     6601 agcattcaga cgtacgccac aagcaccttc catatagagg atcggggcga cgcgcgcttt
     6661 cacgccttcg agacgagcga tacgggtcat cagtgcctta cgtgccagca ccagacgttc
     6721 atccagcagc ttccagaagg tggcttcatc gccttttgct tccagagcaa tacgcggcag
     6781 gttcaggctg atcacgccga ggttgttacg accatcgtgg atctgctcgc cgttttcatt
     6841 ttcccacacg ccgaggaagc tgcggcagcc catcggggtt ttaaacgaac cggtgacgtt
     6901 cactacctga tcgtagttca ggatatccgg atacatgcgc ttgcttgcgc actccagcgc
     6961 cagctgtttg atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat cgcgaatcgc
     7021 aaacaccagt ttcgggaaca ccgcagtttt acggttttta ccgaggcctg cgatacggtt
     7081 acgcaggatc gattcctgaa tcaggcgcga ttcccagctg gtgcccagac caaaaccaaa
     7141 ggttacaaac ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt actccagcga
     7201 ctggaaggca tcgtagcact ctttgatggt acgagagtta gcgtagcctt cggcgtccgg
     7261 gatgttccac tcttctgcgg tcttgcgatg tttgttgtag ctggcggtaa caaacggcgc
     7321 cagcacttca tcgatacggt taatggtggt gccgccataa atatggctgg cgacctgagc
     7381 aataatctgc gcagttaccg cggttgccgt agagatcgac ttcggcggtt caatctcggc
     7441 gttccccatt ttaaaaccct gggtcagcat gcctttcagg tcgatcaaca tgcagttgaa
     7501 catcgggaag aacggcgagt aatcgagatc gtgatagtga atatcgccac gctcatgtgc
     7561 ctgcaccacg tcacgcggca gcaggtgctg gcgtgcatag tgtttagcca cgatcccggc
     7621 cagcaggtcg cgctgggttg gaatcacctt gctgtctttg ttggcgtttt cgttgagtaa
     7681 cgaggcgttg gtctgctcga ccagaccacg gatctcctgg ttcaggcgac cgcgtttttc
     7741 acgttcaatg tcgcgatcgt gacggtactc gatgtaagca cgagccagtt gtttgtatgg
     7801 acccgacatc agctggtttt caactgcggt ctggatctcg ttgatatcca cctggttgcg
     7861 cccctgcatc tgctcgttga caaccgcggc aacagtggcg caataatcgg catcatcgac
     7921 ttccgctgct ttagctgcac gcagaatcgc ttctttgatg cgctctgatt taaacggcac
     7981 tttgcagccg tctcgtttca tcacatgcgg tgtcatgatc gctccatatt tttaagaaca
     8041 ggttatccac agaaattggg aaaggcgttt tccggttttt tcgtttcgtt ttccggtgct
     8101 ttccgcaatc gccatccgct tatccacaac gccgcacctt tttcgtgggg agctgtagta
     8161 gcaattatag tcgattaata caacatattg ggttgggacg cattttaaag tctatatata
     8221 gtgctttgca tcaaggatgt tttaactttt tttgatgtag ctcaaagtaa aaagcagagc
     8281 gtacggatga cgggcgctac agcgttatgt aaatttttta atgaatttgc tggttgaaaa
     8341 atcaacaaaa acaacatgct gacagacaaa accccggaat gaccggcatt atcggggctt
     8401 agggaagatt tacttctgta accaccagac agcctcaaaa ggccgtaaat tcatggcaca
     8461 gggttgtggt gaggcttctt cgtagttatg catcacaagc tgccagttgc cgcgcatttg
     8521 ccctggctgc cagggttgga tctcacggct aaggttggca atgaccagca aggtttgccc
     8581 cttccattca cggcgatagc accacaatac agggctgttt ggcagcagat cctggtaatt
     8641 gccccatgtc aggatggctt cttgcttacg cagtgcgatt aacttttggt aggtgtaaaa
     8701 cacagaggag tcatcggcca gcgcggcttc tacgttgatt tgttgatagt tatcgcctag
     8761 gccaatccac ggttcgccag ccgtaaaccc ggcattatcg ccgttgctcc attgcatggg
     8821 cgtgcggctg ttgtcacggg atttactggc gaggattgcc aataactcgt cggcatcacg
     8881 accatcgttg cgcagctcgg caaacatatt gaggctctcc acgtcgcgat agtcagtaat
     8941 gcgcgtgaaa tgcgggttgg tcatgccaat ctcttcgccc tggtagatat acggcgttcc
     9001 ctgcatgcca tgcagcacca tcgccagcat ttttgccgca ggcacgcggt attcaccttc
     9061 atcaccaaag cgagaaacaa tgcgcggctg atcgtggtta caccagaaca aggcattcca
     9121 tgccacgttg tgcattcctt gttgccagtg gcggaacaat gttttcaacg ccacaaagtc
     9181 aggtttagcc agcgtccatt tttcaccacc gggataatcg accttcaggt gatgaaaatt
     9241 aaaggtcatc gacaattcac tgcctgtcag tgccgcgtat cgctggcaat gctcaaggct
     9301 ggtggaggac atttcaccta cggtcattaa cccgcgtggc gtaaacacat cgcggttcat
     9361 ctcgtgcaaa aactcgtgtg ctcgtggccc gtcggtgtag aagcgacgcc cgtcgccatc
     9421 caggtcttca gggaaacgcg ggtctttgga gatcagattc accacatcca ggcgcaaccc
     9481 gtcgacccca cgatcggccc agaattcaca gacttttttc agctctgcgc gtaccgctgg
     9541 attctcccag ttgagatccg cctgttctgg tgcaaagaga tgcaaatagt actgttcgct
     9601 ttccgcatgc cagcgccacg cactaccgcc aaattttgaa cgccagttgt tcggtggcgt
     9661 ttctggttcg ccatcgcgcc agatataaaa ctggcggtaa gggctttctt tgttcagcgc
     9721 ctcgcgaaac caggcatgtt gggtagaggt atggttaaac accatatcga gaatgatacg
     9781 aatcccgcgc gattttgcct gcgtcaccag ttcgtcaaaa tcgtccagcg tgccgtaggt
     9841 gggatcaatc gccgtatagt tcgctacatc gtaaccgtta tcgacctggg gagagacata
     9901 aaagggggtt agccagatgg catcaacgcc cagtttatgc agatagtcca ggcgttggat
     9961 aacgccacgt aaatcgccgg tgccgctacc cgtggtgtcc tgaaaactct ttggataaat
    10021 ctggtagata acgccgtttt gccaccagtg gggaagatta gtcattacgt tattcctgca
    10081 aatgcgaggg ggcgcaattg cgccccgaag aaaattaaac aatgtccagc gtgcccaggc
    10141 ggtatttccg ctgatagata aacgaggtga gtacaatcgg gatgatgatg gcgatagcca
    10201 ttgccagcgc aaatacctgc cagtagctcg gttgaataga gagaattccc ggcaggcccc
    10261 ctacgccgat accattcgcc ataacgccgt tcaggccgca tagcaatcct gccagaccag
    10321 aaccaatcat cgcgcacagc atcgggaagc gatatttcag gttgatgccg tacattgcag
    10381 gctcagtgac cccaagccag gcggagatag cggcaggcac ggagatctcg cgttcattgt
    10441 gcttgcggct ggaaatgata atgcctatca cggcggagcc ctgagcgata ttcgacagcg
    10501 caatcagcgg ccacactggc gtgccaccca tgctttgaat catctgcaaa tcaatagcaa
    10561 gggtggtctg gtgtacgccg gtgatcacca gcggcgcgta caggaagcca aacaatgccg
    10621 caccaatcgg agcaaagctg ccggtcatca ggtgacgtac cgcaaaggca acgccatcgc
    10681 caatcatgcg accaaacgga ccaatcagcg catgggcgag gaacaccgcg aggatcagcg
    10741 aacagacggg caccaccacc agatagaggt aatccggtac gatgcgttta aggcgagttt
    10801 caataacgcc cagcgccagc ccggctaaca gtgccgggat cacctgcgcc tgatagccca
    10861 ctttggcgat gctgaacatg ccaaagtccc acacttccgg cagctgctgc ccgagcagat
    10921 aagcgttcat cagctggggg gaaaccagtg tcacgccaag cacgatacca aggatcggcg
    10981 tgccgcccat ttttttcacc gctgaccagc aaatcccgac cggcaggtag aagaaaatcg
    11041 cttcaccgat caaccacaga aaatcgtaga tcgtttgcag ggaagggtac atttgcgcca
    11101 gcgtctgacc gttgctcatg ggcaaatcgc cgatcacatt gcgaaaaccg aggatcaaac
    11161 cgccgctaat caacgcgggc agcaacggga agaagatctc cgcgaaatga gagatcaact
    11221 gctcatgcca tttcatattt tgccgggcgg ctttttttac ctgctcttta tcaacctgcg
    11281 cctgtccggt tgacgctatt agtgcttgat agtaatcacc cacgttggtg ccaatcacca
    11341 cctgaaattg cccggcattg gtgaaacagc ctttcaccat ggggagttgc tcaatttctt
    11401 tcggtctggc attggccggt tggttgagga caaagcgcag gcgagtaata cagtggctca
    11461 ccgtcgcaat attgccgcgc ccgccgacca gttcaatcaa ccgatcgata tccgtttggt
    11521 ttattttgct catcataaag ccccatggca gatgacattt ttggttggct gcagaatata
    11581 ttgcgcggaa aaatttaaaa acgggaacgt tcccgaaacg cagcgaagat cacaatttat
    11641 cgttcaggaa acgatcagga cagggtggcg gggatgatga tttgttgcgg ttcgctgcgc
    11701 ccggttacct gtgcgatcaa ttggcaggcc gcctggcgtc cagcttcggc gtagccagga
    11761 tctacggtga cgatctccgg atgaaggaat ttcattaacg gcgtattacc tacgctcgcc
    11821 agttgcaagg tgtcgatgcg ttgctcttgc aggtatttac ttgcgccaag agccagcgtg
    11881 tcggttgcgc acagtaacgc ggtagtttca ggcgtaatca cttttgctac gttttcatag
    11941 ccttgcttca tagcaagccc tggcagagcg gcaacgggat gcagtttatg cgctttgcag
    12001 aacgccaggt aggcttcgtg acgtcgcttc ccggtcgtca cgtcactatg cggcacgccg
    12061 agataactga tattgcgatg cccctggtca tagagccgtt gcatcagaat cttgattgcc
    12121 ccttcgtcgt cataacagac cgaagcaaag ccttttgcgt cacgcgccag cagaaccagc
    12181 gatgactgcc agtgggctaa catttcttcg gttatgccgg taaaaccgaa cagcactacg
    12241 ccgtcgatat tacgccgttt cagcaccccc aaatgttcgg caactaattg tggggaaaac
    12301 tgattttcca tcatgattgg atcgtaacct tgttcataga acgctggcag catggtttga
    12361 acggcgagat tttctgacaa cgaatccaga cgggtaacaa tgatggcgac cactttatcg
    12421 ctttgcccac gcatagcgcg cgcagagcgg gaaggggaaa atccatgctg attcatcact
    12481 gcttcaacac gctcgcgcgt gcgctgactc acgccgcttt cgttattcag cacccgggaa
    12541 accgtagatt tccccacgcc gcttaagcgc gcgatgtctt tgatggtcag ccgattttgc
    12601 atcctgttgt cccataacgt gttgtttaat tatttgagcc taactttacc cgtgcattca
    12661 gcaatgggta aagtctggtt tatcgttggt ttagttgcca gcaggtatta tatcgccata
    12721 aatgctacga atattattgg attctcctta ttatttgcgg cgcttttttc acttaccgga
    12781 ggttatatgg aacctgatcc cacgcctctc cctcgacgga gattaaaact tttccggtaa
    12841 gcccgtcttt tcacggcgtt accggatgcg taaggccgtg acgttttaat gtccctgctc
    12901 agctttatta ccttcaggta aggcttcgcc acgcctgaag acatttctgt actgtttcag
    12961 acagtgcgga gggactcctt atgtttaaag aaatttttac ccggttcatt cgccatttac
    13021 cttcccgtct ggttcatcgc gatccattgc ctggcgcgca gcagacagtg aatacggcgg
    13081 tcccgccgtc cttaagtgcg cattgcctga aaatggcggt gatgcccgaa gaagaattgt
    13141 ggaaaacgtt cgacacccat ccggaaggat taaatcaggc ggaagtggaa tctgcccgcg
    13201 aacaacatgg tgataataaa ttacccgccc aacaaccgtc gccgtggtgg gtacatttat
    13261 gggtctgcta tcgcaacccc tttaatattt tgctcaccat tcttggtgct atttcttacg
    13321 ccacggaaga tttatttgcc gcaggtgtta tcgcgctaat ggtcgctatt tctacgttgc
    13381 tgaactttat tcaggaagct cgttccacca aagcggcaga tgccctgaaa gcgatggtca
    13441 gtaataccgc gacggtactg cgagtaatta acgacaaagg cgaaaatggc tggctggaga
    13501 tcccgataga ccagctggtg cccggcgata ttataaaact ggcggcgggg gatatgatcc
    13561 cggcagattt acgtatcttg caggcgcggg atctgttcgt cgctcaggcg tcgttaaccg
    13621 gtgagtctct gcccgtggaa aaagccgcta ctactcgcca gccggagcac agcaatccgc
    13681 tggagtgcga caccctgtgt tttatgggca ccaccgtggt gagcggcacg gcacaagcaa
    13741 tggtgattgc tacaggagcc aacacctggt ttggtcaact ggcggggcgt gttagtgagc
    13801 aggaaagcga gccgaatgcc tttcagcaag ggatcagccg cgttagtatg ctgctgattc
    13861 gctttatgct ggtgatggct ccggtggtgt tgcttatcaa cggttacacc aaaggcgact
    13921 ggtgggaagc ggcactgttt gcgctttcgg tcgcggtagg cttaacgccg gaaatgttgc
    13981 cgatgattgt tacctcgacg ctggcgcgcg gcgcagtaaa gctgtcgaaa cagaaagtga
    14041 tcgtcaaaca tctggatgcc attcagaact ttggcgcaat ggatattctg tgcactgata
    14101 aaaccggcac cctgacgcag gacaaaattg tgctggagaa tcataccgat atctccggta
    14161 aaaccagcga acgcgtgctg catagcgcgt ggttgaacag tcattaccag accggactta
    14221 aaaatctgct tgataccgcg gtgctcgaag gtacggatga agagtcagcg cgttcgctgg
    14281 ccagccgttg gcaaaaaatt gatgagattc cgtttgattt cgagcgtcgt cggatgtcgg
    14341 tggtagtggc agaaaatacc gaacaccatc agctggtttg caaaggtgca ttgcaggaaa
    14401 tcctcaatgt gtgttcgcag gtgcgtcaca atggcgagat tgtaccgctc gatgacacca
    14461 tgctgcgtaa gattaagcgg gttactgata cgctgaatcg tcaggggctg cgcgtggttg
    14521 cggtggcgac gaaatacctg ccagcgcgtg aaggagatta ccagcgggcg gatgaatccg
    14581 acctgatcct cgaaggatat attgctttcc ttgatccgcc aaaagagaca accgctccgg
    14641 cactgaaggc attaaaagcg agtggaatta ccgtaaaaat ccttactggc gacagtgagt
    14701 tagttgcagc gaaagtgtgc catgaagtgg ggctggatgc gggagaggtg gtcattggta
    14761 gtgatattga aacgctatct gacgatgaac tggcaaatct cgcacagcgc accacgctgt
    14821 ttgcccgcct gacgccgatg cataaagaac gcattgtgac tttactgaag cgcgaagggc
    14881 atgtggttgg ctttatgggc gatggtatta atgatgcgcc cgccttacgc gctgcggata
    14941 tcggcatttc tgtggacggc gcggtagata ttgcccgtga agcggctgat atcatcctgc
    15001 tggaaaaaag cctgatggtg ctggaagagg gggttattga gggacgtcgc actttcgcca
    15061 acatgctgaa atacatcaaa ttgacggcga gttctaactt cggtaatgtg ttcagcgtgc
    15121 tggtggcgag tgctttcttg cccttcctgc cgatgttgcc gttacacttg cttattcaga
    15181 acctgctgta cgatgtgtca caggtggcga tcccgtttga taacgtcgac gacgagcaaa
    15241 ttcaaaagcc gcagcgttgg aatccggcgg atctggggcg ctttatggtc ttcttcggac
    15301 cgatcagctc gattttcgat attctgacgt tttgcttgat gtggtgggta ttccatgcca
    15361 acacgccgga aacgcaaacg ctgttccagt cgggctggtt tgtggtggga ttactgtcgc
    15421 aaacgctgat tgtgcatatg atccgtaccc gccgtgtgcc gtttattcag agctgtgcat
    15481 cgtggccgtt aatgatcatg accgtgatcg tgatgattgt cgggatcgca ttgccgtttt
    15541 caccgctggc cagttatctg cagctgcagg cgctgccgtt aagctatttc ccgtggctgg
    15601 ttgcgattct ggcagggtat atgacgttaa cccagttggt gaaagggttc tatagccgtc
    15661 gttacggctg gcagtaaaga ataaactggg catggtggcc cggattcaga ttgcagacaa
    15721 agtgagcttt gttcatgccg gatgcggcgt aaacgcccaa tccggcctac aaattcaata
    15781 aattgcagga atcacgtagg cctgataagc gtagcgcatc gggcgatttt gcttttgtca
    15841 tcagacttaa tccgggcatg atagcccgga tttccatcaa gattagcgac gaacagcgat
    15901 tgcttcgatc tcaatcttca cgtctttcgg cagacgggca acttcaacgc aagaacgtgc
    15961 cgggaaagtg gcgttgtgtt cggtgaagaa ggcttcgtaa gtggcgttta cggttgcgaa
    16021 gtcgttcaga tctttcacga acacagtggt tttaacgatg tcgcccactt tcaggccagc
    16081 ggcttcgacg attgctttta cattatccag cgactgacgt gcctgtgcag cgacgtctgc
    16141 cggtacttca ccagttttcg gatttaccgg gatctgaccg gaggtgatga tcatattgcc
    16201 cagatcaacg ccctgtacgt aaggaccgat agctgccggt gcattttccg tcgcgatagt
    16261 tttgctcatg atttctcctt tattacagcg gtaaaaagtc tggctcatta tagggagcca
    16321 gacttttatt accaaccgca attaattggc cagcaccaca ttatgggaaa actctttttc
    16381 acagtatttg catttaagcg cgatgtcatt ggcgcgtttt cgcacggcaa agctggatga
    16441 aaccggttcg gcatggctga tacagttgct gttcgggcag accagcacat tgtcgatgcg
    16501 ctctggcaga cttgggcgcg atttacccac cacttcatag ttgtcgatac ggttaaccgt
    16561 ggcttgcggt gcatacaatg ccagttggtc tacttgctct tcactcaaaa aggtattttc
    16621 gattttgatc agatctttgc ggcccatctc gccagaaggc aaattcagac caatggtgat
    16681 gcgctgatct gtttcggtca acttgaacag actcaacagc ttaaaaccga tctgggcggg
    16741 gatatggtca attaccgtgc cgcgtttaat agcttcaacc tgcaatttat tatcgtgtgt
    16801 catctctatt tccccttaca gtaccagatc gcgattcaga accagtgcca gtaacgcctg
    16861 gcgagcgaaa atcccgttgc ctgcttgctg gaagtaccag gcgtgaggcg ttttatccac
    16921 atccgtcgca atctcatcaa cacgcggcag cggatgcagc actttcatat tggctttggc
    16981 gttgtggaga tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt actcggacgg
    17041 gtccagacgc tctttttgca cgcgggtcat gtacaggatg tctacttccg ccatcacttc
    17101 ttcaatagag ctgtgcagac tccaggcgat ccctttttca tcgagcatat ccagaatgta
    17161 ttgcggcatt gccagcgcat ccggcgcgat aaagtaaaaa cggttgccgt cgaacttcgc
    17221 tagcgcctgg gtcagggagt gaacggtgcg gccatatttc aggtcaccaa ccattgcgac
    17281 gtggagattg tccagacgcc cctgggtttc ctgaatggtg aataagtcca gcaaggtttg
    17341 cgtcggatgt tggttggagc catcaccggc attcagtacc ggtacattgc cagaaaattc
    17401 ggtggctagg cgtgccgcac cttcctgcgg atgacgcatc actatcgcat cgacgtaagt
    17461 gctgataacc gaaatggtat cggccagcgt ttcgcctttt ttacccagtg atgtattggc
    17521 gctgtcggag aagcccacca cgctggcacc caggcggtgc atggaagttt cgaaagagag
    17581 gcgggtacgg gtagaggctt cgaagaaaca gctggcaatg actttgtgct tcaacagctc
    17641 tggttgcggg tttgctttca gtttcgccgc tgtcgccagc accagattaa ggtcatcgcg
    17701 actaaggtcg tttatggaaa tgatatgttt ctgatatagc ggattagcca tcttttatct
    17761 cctgacacct aggcaaaaaa aagcccctcg attgaggggc tgggaatggg tgatcaacgg
    17821 gaagaaaaac ggcaggccag cgtctttttt cagacgcggt aagacaaaat gtcgaacaca
    17881 ctgaaccata catcctcccg gcaaattgtc cggcattata ctcatagcca gaagcggtgc
    17941 aagcatttga tgcaatattt tgtcagcgca aacggtttat ttgaattaaa agtcaaaata
    18001 tatgcatttt tattttctat gattctggag gggaagtgtc gttcggtatc aataattgca
    18061 gacatttcct gcttttttct ttttcatttt cacgcaatca gaaatgcatt ttattccatt
    18121 catccgactt atagggcgga gggtgaaaac gaacggctaa cactattgct tatagctcag
    18181 ggatacgcgc tatcacttta atttcaaaat caaagcctgc cagccaggta acacccaccg
    18241 ccgtccagtt tggataaggt ggggcgctaa atatttcatt tttcaccgtc atgatgtctt
    18301 cagattgttt ttccggatcg gtatgaaagc tcgtaacatc aatgatatcg tcaaaagtgc
    18361 atcccgcagc tgccagggtc gcatgcaaat tatcaaaggc cagtctgact tgttgctgaa
    18421 aatcgggttc tggtgttccg tcctctcgac ttcctacttg cccggaaaca aacagcaaat
    18481 cgccggaacg aatagccgca gaataacgat gctcagcata cagtgaatgt cggccagcag
    18541 ggaaaacagc ggttctttct accatttggt tatcctcaag atttacgaca tgaacagaag
    18601 atttatattt atcgggagcc gcttttaggg ggacgacgtt agtaaacaaa acccagacat
    18661 catggataat ggctgggctt aattgagcgt agtcggttat gcgccaaacg cgccatcaat
    18721 ggtatgcatc gcgccggtaa caaaactggc ttctggccct gctaaccatg cgaccatacc
    18781 agcgacctct tccggttgcc catgtctttt gatggccata aaactatgca acatatcgcg
    18841 cattggcccg ttggcgggat taacgtcggt atcaattggt cctggctgga cgacgttgat
    18901 ggtgatccct cgcggtccaa aatcacgggc cagcccgcgc gccatgcctt gcagggcaga
    18961 tttactggcg gcataagcag ccatgcctgc aacaggcata cgatcgccat tcacggagcc
    19021 gatgattaag atgcgcccgc cttcgggcat ctgccgggcg gcttcaacag aggcatgata
    19081 aggagcatga atattgattt tgaaaaggcg atcaatatcg tcggcattta attccagggc
    19141 atcgccaaag acgccaatac ctgcatttac caccaggata tccaatgcgc cgctcttacg
    19201 aacgacatca ataacagcgt ctctgtcagc actatctgtg aatactgctg tcgctccagt
    19261 ctcttgtgcc agacgttcag cggcatcttt cgaccccgca taggtgaatc gtacattggc
    19321 cccatcggtg acgaaacgac gtacgatagc ggcaccgatt ccacgactgc caccgaggat
    19381 gagaactgtc ttaccggtaa aagcgcccat aaggactcct tgatttatta tgtaacatgc
    19441 attacaaaac tgttttaact ttctgtcaac aggttttgta atggtcacta aaaaacaatc
    19501 tcgcgttcca ggtcgtccca gacgtttcgc tcctgagcag gcagtctctg cggcaaaagt
    19561 gctttttcac caaaaaggtt tcgatgcagt aagtgtctct gaagttactg attatcttgg
    19621 tattaacccc ccgagtctct acgcggcttt tggcagtaag gctgggttat ttagccgtgt
    19681 actcaatgaa tatgtcggta cggaagctat tccgcttgcc gatattcttc gtgatgatcg
    19741 tccggtaggc gagtgcctgg ttgaggtatt aaaagaagcg gcgcgcagat atagccaaaa
    19801 cggcggctgc gctggctgta tggttcttga aggtattcat agtcatgatc cacaagcgcg
    19861 tgatattgcc attcaatatt atcacgccgc agaaacgacc atttatgact atatcgccag
    19921 gcggcatcca caaagcgcac aatgtgtgac tgattttatg agtaccgtga tgtcagggct
    19981 ttctgcgaag gcacgggagg ggcactccat cgaacaactc tgtgcaacag ctgccctggc
    20041 aggggaagcg ataaaaactc ttctcaagga gtgatgctgg ccttgatccg aaaggcggga
    20101 acgcgcctgc cgataagttg tgataagaca ataattcacg cgttaaggct ggcggaattg
    20161 atcatctttt cgtataacga tagaaatgaa acattgtttt aattaaggag tggaaaagat
    20221 gatcatcgga aatattcata atcttcagcc gtggctaccc caggagttac gccaggcgat
    20281 tgagcatatc aaagcacacg ttacggcaga aacgccaaag ggcaagcatg atatcgaagg
    20341 caatcgtctg ttttatctta tctcggaaga tatgaccgag ccgtacgaag ctcgccgtgc
    20401 ggagtaccat gcccgctatc ttgacattca gattgtgtta aaaggtcagg aaggcatgac
    20461 cttcagcacg caacctgcag gcgcgccgga taccgactgg ttagctgata aagacatcgc
    20521 atttttgccg gaaggcgttg atgagaaaac agttatccta aatgaaggtg attttgttgt
    20581 gttttatccg ggggaagtgc ataaaccgct gtgcgcagtg ggcgcgccag cccaggttcg
    20641 caaagcagta gtgaagatgc tgatggcgtg atgacttttc gccgtaaata actccaggtt
    20701 tacggcgagt ttgtgaaaag agcgtttttt gatatttttt tgtgagtaaa atttgtaatg
    20761 cttagacgtt cttattaact caaggagttc gtcatgggca aaatatcaga tttgaattat
    20821 tctcaacaca ttacattagc cgacaatttt aaacaaaaaa atgaagcctt agatacctgg
    20881 tatgtgggaa tgaatgattt tgcccgaatt gccggggggc agaatagcag aagcaatatt
    20941 ctttctccta gagcattttt ggagtttttg gcaaagatat ttaccctggg ttatgtggat
    21001 tttagcaaac gctccaacga agcgggtaga aatatgatgg ctcatattga gttctcatct
    21061 tattctaaag atactgatgg caatgaaaaa atgaagtttt acatgaataa tcctgaaggg
    21121 gagcgagcgg atttatctaa ggtgaagata gaaattacac ttgcctcagc ttctactaaa
    21181 gggattcgtg aaggacatac agtcattata tttaaacaat ctgatggttc gactaatcgt
    21241 tatgaaggga agtcttttga aagaaaagat gatagttcat tacacctgat tactaacaag
    21301 gttctggcgt gttaccaacg cgaagctaac aagaaaatag cgcgtctatt aaataatcat
    21361 cagaagttaa ataatataca ggagttaaat gattcgcagg agttaaataa ttcacagaag
    21421 ttaaataatt cgcaggagtt aaataactcg caggtgagtt gtaaaggttc agttgattct
    21481 acgattacgg atttattaga aaaagcattg aa
//
LOCUS       DAJTSA010000065        21503 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000065 DAJTSA010000000
VERSION     DAJTSA010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.065"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="OO369_002622"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="OO369_002622"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR5982851.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEVLLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="OO369_002623"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="OO369_002623"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR5982852.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="OO369_002624"
     CDS             complement(3121..3420)
                     /locus_tag="OO369_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR5982853.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /gene="wecH"
                     /locus_tag="OO369_002625"
     CDS             3595..4590
                     /gene="wecH"
                     /locus_tag="OO369_002625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR5982854.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /locus_tag="OO369_002626"
     CDS             complement(4632..5069)
                     /locus_tag="OO369_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR5982855.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /locus_tag="OO369_002627"
     CDS             complement(5115..5456)
                     /locus_tag="OO369_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5982856.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="OO369_002628"
     CDS             complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="OO369_002628"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR5982857.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="OO369_002629"
                     /pseudo
     CDS             complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="OO369_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8839..9831
                     /gene="xylF"
                     /locus_tag="OO369_002630"
     CDS             8839..9831
                     /gene="xylF"
                     /locus_tag="OO369_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5982858.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9909..11450
                     /gene="xylG"
                     /locus_tag="OO369_002631"
     CDS             9909..11450
                     /gene="xylG"
                     /locus_tag="OO369_002631"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR5982859.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11428..12609
                     /gene="xylH"
                     /locus_tag="OO369_002632"
     CDS             11428..12609
                     /gene="xylH"
                     /locus_tag="OO369_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR5982860.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12687..13217
                     /locus_tag="OO369_002633"
     CDS             12687..13217
                     /locus_tag="OO369_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR5982861.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13329..13865
                     /locus_tag="OO369_002634"
     CDS             13329..13865
                     /locus_tag="OO369_002634"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5982862.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13973..14797)
                     /locus_tag="OO369_002635"
     CDS             complement(13973..14797)
                     /locus_tag="OO369_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR5982863.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15117..17147
                     /gene="malS"
                     /locus_tag="OO369_002636"
     CDS             15117..17147
                     /gene="malS"
                     /locus_tag="OO369_002636"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5982864.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17325..18578
                     /gene="avtA"
                     /locus_tag="OO369_002637"
     CDS             17325..18578
                     /gene="avtA"
                     /locus_tag="OO369_002637"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR5982865.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18729..19202)
                     /locus_tag="OO369_002638"
     CDS             complement(18729..19202)
                     /locus_tag="OO369_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5982866.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="OO369_002639"
     CDS             complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="OO369_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR5982867.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20352..21350
                     /gene="yiaK"
                     /locus_tag="OO369_002640"
     CDS             20352..21350
                     /gene="yiaK"
                     /locus_tag="OO369_002640"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR5982868.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21362..>21503
                     /locus_tag="OO369_002641"
     CDS             21362..>21503
                     /locus_tag="OO369_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5982869.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT         5402 a         5491 c         5277 g         5333 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagta cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaaccct tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 ccccccgtac ggtaatcgag ctgctgaccg ctgatatccg ccagcatctg acgccagtac
     5881 tcactacgcg ccccgccacc aatcaacgta acactttgcg gtttaatgcc gcaggcatgc
     5941 acgacatcca tgccatctgc cagcgcataa cccacgcctt ccagcactgc tcgcgccagt
     6001 tcattggggc catgttgatg agtcaaacca aagaaaaccc ccttcgcctg gggattattg
     6061 tgtggtgtac gctcgccgga aagataaggc agaaaccaaa ctggctcggc actttcatca
     6121 gcctgttgag ctgcagcgat taaagctggg acattgcaca ggccggttaa tttcgcggcc
     6181 caatccagac acgatgctgc actcagcatt acagacatta aatgccaacg ctgcggtagc
     6241 gcatggcaaa agctgtgtac agcgctttct ggcttgctta agaacccttc gctgacagca
     6301 aaatagaccc ccgacgtccc cagcgataac attgcctgat tagcatcaac cattcccaca
     6361 ccaactgcac cagctgcatt gtcgccaccg cctgcgacaa ctggcaccgt cgccataccc
     6421 cacgctttcg caacttcagg taacaaagca ccagtaattt cgctgccttc gtataatgcg
     6481 ggcatctggt cacgagataa gtcgcaagcc tgcagcatga cgtcactcca gtcacgcttt
     6541 gcgacatcca gccacatggt gccagctgcg tcagacatat cgctggcaaa ctcccccgtc
     6601 atacgcagac gcaagtaatc tttcggtaat aatactttgt cgatttgacg gaatatctcc
     6661 ggctcatgcc gctgaaccca tagcaattta ggcgcagtaa atccgggcat catcaggttg
     6721 ccggtaatca ctcgtgattg cggaactctc gcttccagca aagtgcactc ttgcgcacag
     6781 cgcccgtcgt tccacaaaat ggcagggcgc aatacccgtt gttgagcatc cagtaaggtt
     6841 gctccatgca tctggccggc aatacccaat gctttaacgt cctgcagaga atgctgatcg
     6901 cccagagctt tcattgcgcg atcagttgcc tgccaccact gttccgggtc ttgttccgac
     6961 caaagtggat gcgggcgcga aacggtcagc ttttccgttt gcgaagcaac cacctcaccc
     7021 tgctcgttga gcaaaataac ttttacgccc gaggtgccaa gatctatccc gatatacata
     7081 tcgatcgttc cttaaaaaaa tgcccggtat cgctaccgat aaccgggcca acggactgca
     7141 cagttagccg ttatttgtcg aacagataat gatttaccag attttccagt tgctcctggc
     7201 gaccactctg atgcaccgga gacaaattat gttcctgagc atatttggct aaatctgcca
     7261 gtgacatttg gcctttcagg atttgctggc ccaattcgct attccagccg gaataacgct
     7321 gcgcgatgcg tttatccagc tcgccatctt caatcatgcg cgctgcaatt ttcagcgcca
     7381 gtgccatcgt atccatcgcg ccgatatgac cgtaaaacag atcatattta tcagtacttt
     7441 gacgacgtac tttggcatcg aagttcagac caccggtggt gaaaccgcct gctttgagaa
     7501 tttcatacat caccagcgca ttctcttcca cactgttcgg gaactggtcg gtgtcccagc
     7561 ccagttgcgc atcgccacgg ttggcgtcga cagaaccgaa caggccaagc gcaatggcgg
     7621 tggctatttc atgatggaaa gagtgacctg ccagcgtcgc gtggttagct tcaatgttca
     7681 gtttaatctc tttttccaga ccaaactgtt tcaggaagcc atagaccgtc gcggcatcgt
     7741 aatcatattg atgtttagtc ggttcttgcg gtttcggttc gataagcaac gtgccctaga
     7801 agccgatttt atgtttatgc tcaaccacca tctgcataaa gcggcccagt tgttcacgct
     7861 cctgacgcaa gtcggtattt aacagcgttt cgtaaccttc acgaccaccc cacaggacat
     7921 agttttcacc gcccaattta tgggttgctt ccatcgctgt aacaacttgc gttgccgccc
     7981 agctgaagac ttcaggatct gggttcgtcg ccgcacccgc gccatagcga gggtttgtaa
     8041 agcagttagc ggttccccac agcagcttca cgccgctctc ttcttgcttg gctgccagga
     8101 catcaaccat ttgcgcaaaa ttattgatgt actcttttaa cgacgcgccc tcaggggaaa
     8161 catccacatc gtggaagcaa taaaatggca catgtaactt gtggaaaaac tcaaatgcga
     8221 catctgcttt acgcttcgcc aacgccagtg cctcaccagg ctgctgccac ggacgattaa
     8281 acgcccccac accaaacatg tccgccccgt tccagcagaa ggtgtgccag tagcaggcgg
     8341 caaaacgcaa gtgctcttcc atacgcttac ccaacaccag ttcgtcggga ttgtagtgac
     8401 ggaatgctaa cgggtttgag gattttgagc cttcataacg aacgcgatcg agctggtcaa
     8461 aataggcttg catattgaac tccataatca ggtaatgccg cgggtgatgg atgatgtcgt
     8521 aatattgggc actccctttc aattgctcaa ttatgttatt tcacactgct attgagataa
     8581 ttcacaagtg tgcgctcgct cgcaaaataa aatgggatga tgaaaatggg taattcctcg
     8641 aagataaaaa tgcaataagt acaattgcgc aacaaaagta agatctcggt cataaatcaa
     8701 gaaataaacc aaaaatcgta atcgaaagat aaaaatctgt aattgttttc ccctgtttag
     8761 ttgctaaaaa ttggttacgt ttatcgcggt gattgttact tattaaagct gtcctctaac
     8821 tacagaaggc cctacaccat gaaaataaag aacattctac tcaccctttg cacctcactt
     8881 ctgcttacca acgttgcggc acacgccaaa gaagtcaaaa taggtatggc gattgatgat
     8941 ctccgtcttg aacgctggca aaaagatcga gatatttttg tgaaaaaggc agaatctctc
     9001 ggtgcgaaag tatttgtaca gtctgcaaat ggcaatgaag aaacacaaat gtcgcagatt
     9061 gaaaacatga ttaaccgggg tgtcgatgtt cttgtcatta ttccgtataa cggtcaggta
     9121 ttaagtaacg ttgtaaaaaa agccaaacaa gaaggtatta aagtattagc ttacgaccgt
     9181 atgattaacg atgcggatat cgatttttat atttctttcg ataacgaaaa agtcggtgaa
     9241 ctgcaggcaa aagccttggt cgatattgtt ccgcaaggta attacttcct gatgggcggc
     9301 tcgccggtag ataacaacgc caagctgttc cgcgccggac aaatgaaagt gttaaaacct
     9361 tacgttgatt ctggaaaaat taaagtcgtt ggtgaccaat gggttgatgg ctggttaccg
     9421 gaaaacgcat tgaaaattat ggaaaacgcg ctaaccgcca ataataacaa aattgatgct
     9481 gtagttgcct caaacgatgc caccgcaggt ggggcaattc aggcattaag cgcgcaaggt
     9541 ttatcaggga aagtagcaat ttccggccag gatgcggatc tcgcaggtat taaacgtatt
     9601 gctgccggta cgcaaactat gacggtgtat aaacctatta cattgctggc aaatactgcc
     9661 gcagaaattg ccgttgaatt gggcaatggt caggagccaa aagcagatac ctcactgaat
     9721 aatggcctga aagatgtccc ctcccgcctc ctgacaccga tcgatgtgaa taaaaacaac
     9781 atcaaagata cggtaattaa agacggattc cacaaagaga gcgagctgta agcgttacgc
     9841 cccggcgcgg agcgggggcg tgatttctct ccatgccgcg tgaatgaatt ggcttaggtg
     9901 gagtcgttat gccttatcta cttgaaatga agaacattac caaaaccttc ggcagtgtga
     9961 aggcgattga taacgtcagc ttgcggttga atgctggcga aatcgtctca ctctgtgggg
    10021 aaaatgggtc tggtaaatca acgctgatga aagtgctgtg tggtatttat ccccatggct
    10081 cctacgaagg cgaaattatt tttgcgggag aagagattca ggcgagtcac atccgcgata
    10141 ccgaacgcaa aggtatcgcc atcattcacc aggaattggc cctggtgaaa gaattgaccg
    10201 tgctggaaaa tatcttcctg ggtaacgaaa taacccacaa tggcattatg gattatgacc
    10261 tgatgacgct acgctatcag aagctgctcg cacaggtcag tttatccatt tcacctgata
    10321 cccgcgttgg cgatttaggg cttgggcaac aacaactggt tgaaattgcc aaggcactta
    10381 ataaacaggt gcgcttgtta attctcgatg aaccgacagc ctcattaact gagcaggaaa
    10441 cgtcggtttt actggatatt attcgcgatc tacaacagca cggtatcgcc tgtatttata
    10501 tttcgcacaa actcaacgaa gtcaaagcga tttccgatac gatttgcgtt attcgcgacg
    10561 gacagcacat tggtacgcgt gatgctgccg gaatgagtga agacgatatt atcaccatga
    10621 tggtcgagcg agagttaacc gcgatttacc ctaatgaacc acataccacc ggagatgaaa
    10681 tattacgtat tgaacatctg acggcatggc atccggtcaa tcgtcatatt aaacgagtta
    10741 atgatgtctc gttttccctg aaacgtggcg aaatactggg tattgccgga ctcgttggtg
    10801 ccggacgtac cgagaccatt cagtgcctgt ttggcgtgtg gcccggacaa tgggaaggaa
    10861 aaatttatat tgatggcaaa caggtagata ttcgtaactg tcagcaagcc atcacccagg
    10921 ggattgcgat ggtacccgaa gacagaaagc gcgacggcat cgttccggta atggcggttg
    10981 gtaaaaatat taccctcgcc gcactcaata aatttaccgg tggcattagc cagcttgatg
    11041 acgcggcaga gcaaaaatgt attctggaat caattcagca actcaaagtt aaaacgtcgt
    11101 cccccgacct tgctattgga cgtttgagcg gcggcaatca gcaaaaagcg atcctcgccc
    11161 gctgtctgtt acttaacccg cgcatcctca ttcttgatga acctaccagg ggtattgata
    11221 ttggcgcgaa atatgagatc tacaaattaa ttaaccaact cgtccagcag ggtattgccg
    11281 ttattgtcat ctcttccgaa ttacctgaag tgctcggcct tagcgatcgt gtactggtga
    11341 tgcatgaagg gaaactaaaa gccaacctga taaatcataa cctgactcag gagcaggtga
    11401 tggaagccgc attgaggagc gaacatcatg tcgaaaagca atccgtctga agtgaaattg
    11461 gccgtaccga catccggtgg cttttctggg ctgaaatcac tgaatttgca ggtcttcgtg
    11521 atgattgcag ctatcatcgc aatcatgctg ttctttacct ggaccaccga tggtgcctac
    11581 ttaagcgccc gtaacgtctc caacctgtta cgccagaccg cgattaccgg catcctcgcg
    11641 gtaggaatgg tgttcgtcat aatttctgct gaaatcgacc tttccgtcgg ctcaatgatg
    11701 gggctattag gtggcgtcgc ggcgatttgt gacgtctggt taggctggcc tttgccactt
    11761 accatcattg tgacgttggt tctgggactg cttctcggtg cctggaacgg atggtgggtc
    11821 gcgtaccgca aagtcccttc atttattgtc accctcgcgg gcatgttggc atttcgcggc
    11881 atactcattg gcatcaccaa cggcacgact gtatccccca ccagcgccgc gatgtcacaa
    11941 attgggcaaa gctatctccc cgccagtacc ggcttcatca ttggcgcgct tggcttaatg
    12001 gcttttgttg gttggcaatg gcgcggaaga atgcgccgtc aggctttggg tttacagtct
    12061 ccggcctcta ccgcagtagt cggtcgccaa gctttaaccg ctatcatcgt attaggcgca
    12121 atctggctgt tgaatgatta ccgtggcgtt cccactcctg ttctgctgct gacgttgctg
    12181 ttactcggcg gaatgtttat ggcaacgcgg acggcatttg gacgacgcat ttatgccatc
    12241 ggcggcaatc tggaagcagc acgtctctcc gggattaacg ttgaacgcac caaacttgcc
    12301 gtgttcgcga ttaacggatt aatggtagcc atcgccggat taatccttag ttctcgactt
    12361 ggcgctggtt caccttctgc gggaaatatc gccgaactgg acgcaattgc agcatgcgtg
    12421 attggcggca ccagcctggc tggcggtgtg ggaagcgttg ccggagcagt aatgggggca
    12481 tttatcatgg cttcactgga taacggcatg agtatgatgg atgtaccgac cttctggcag
    12541 tatatcgtta aaggtgcgat tctgttgctg gcagtatgga tggactccgc aaccaaacgc
    12601 cgttcttgat tttgataaaa attttctcaa agccggttac gtattaccgg ttttgagttt
    12661 ttgcatgatt cagcaggaaa agaaccatgt ttactaaacg tcaccgcatc acattactgt
    12721 tcaatgccaa taaagcctat gaccggcagg tagtagaagg cgtaggggaa tatttacagg
    12781 catcacaatc agaatgggat attttcattg aagaagattt ccgcgcccgc attgataaaa
    12841 tcaaggactg gttaggagat gtcgtcattg ccgacttcga cgacaaacag atcgagcaag
    12901 cgctggctga tgtcgacgtc cccattgttg gggttggcgg ctcgtatcac cttgcagaaa
    12961 gttacccacc cgttcattac attgccaccg ataactatgc gctggttgaa agcgcatttt
    13021 tgcatttaaa agagaaaggc gttaaccgct ttgcttttta tggtcttccg gaatcaagcg
    13081 gcaaacgttg ggccactgaa cgcgaatatg catttcgtca gcttgtcgcc gaagaaaagt
    13141 atcgcggagt ggtttatcag gggttagaaa ccgcgccaga gaactggcaa cacgcgcaaa
    13201 atcggctggc agactgacta caaacgctgc caccgcaaac cgggattatt gccgttactg
    13261 acgcccgagc gcggcatatt ctgcaagtat gtgaacatct acatattccc gtaccggaaa
    13321 aattatgcgt gattggcatc gataacgaag aactgacccg ctatctgtcg cgtgtcgccc
    13381 tttcttcggt cgctcagggc gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc
    13441 gattattaga taaagaagaa atgccgctac agcgaatttt ggtcccacca gttcgcgtca
    13501 ttgaacggcg ctcaacagat tatcgctcgc tgaccgatcc cgccgttatt caggccatgc
    13561 attacattcg taatcacgcc tgtaaaggga ttaaagtgga tcaggtactg gatgcggtcg
    13621 ggatctcgcg ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg
    13681 caatgattca tgccgagaag ctggagaaag cgcgcagtct gctgatttca accaccttgt
    13741 cgatcaatga gatatcgcaa atgtgcggtt atccatcgct gcaatatttc tactctgttt
    13801 ttaaaaaagc atatgacaca acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt
    13861 tgtagcgatg atgagaattg tcggcgtcac atcagggaac tcagcacagc cacctgatgc
    13921 gagcaaaagc cttatccgac cgacaaatca gataaggctc gaggaaatga aattacatat
    13981 gcgccgcgat taaccgttgg ttatcctggt acattgcgaa caggtagttg ttataactct
    14041 tccctttggt cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat
    14101 ccgctttacg cagctgtgca cgcgatttac ggaacgaaga gtaagccggg tgcgtattca
    14161 ggttagtgac ataggcgctc accgattctt tgacagaact aaactgtgag taccctttca
    14221 ctttacccgg cgcattggta caacgtcctt tcatgcattt catgccgaac agattgttat
    14281 tgttgcgcgc cagcttcgac gttccccaac cgctttctgc tgcagccatc gtcgccacca
    14341 tactggtagg gataatgtct acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat
    14401 taccagacca cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag
    14461 gtgcccattg gccctgatac tgttttgaaa tcagccagtt acgttctgca gtaatggctg
    14521 cattttggct ggtaatgtaa ggcattacgg tccggagaaa cgcctttttc cttggtgttc
    14581 cggaagggta ttttcgcaaa tcaggaagtg aactactctt tgcactattg cgagaatact
    14641 cttgtttact gcttacctgt ttattactag ctttagttaa gtgggacttt tgactcgctg
    14701 ttgttgtgtg cgtcttcgct aacacctcac tcgaaaacac cagagtgagt aacataagaa
    14761 tcatcgcccc atatcgtcgt atgggagtca aaatcatcag gtctcctggt cggatttaat
    14821 cattccaaca ccttatattt ttcacaaatt tgagagttga atctcaaatc atatcaaaaa
    14881 tagctgtcaa gagcacccca aggaatagtc caaatctgaa actatgtcac gagttaacga
    14941 ttcagattgg cgctaaatcg cagaaaatgt gggggttatc gcaaaattca gccgtttttt
    15001 gcgcgcgatc gctcaccctt gcttctcatc ctgtggactt accgctcagg gatgagtttt
    15061 gtctggctta tcgctggcaa actgtctgaa atcgcagcaa taaggactca tccaccatga
    15121 aactcgccgc ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt
    15181 ctccggggtt ccctgccttt agcgaacagg gaacgggaac atttgtcagc cacgcgcagt
    15241 tgcccaaagg tacgcgtcca ctcacgctaa attttgacca acagtgctgg cagcctgcag
    15301 atgcgataaa actcaatcag atgctttccc tgcaaccttg tagcaacacg ccgcctcaat
    15361 ggcgattgtt cagggacggc aaatatacgc tgcaaataga cacccgctcc ggtacgccaa
    15421 cattgatgat ttccatccag aacgccgccg aaccggtagc aaacctggtc cgtgaatgcc
    15481 cgaaatggga tggattaccg ctcacgctgg atgtcagcgc cactttcccg gaaggagccg
    15541 ccgtacggga ttattacagc caacaaattg cgatagtgaa gaacggtcaa ataacgttac
    15601 aacccgctgc taccagcaac ggtttactcc tgctggaacg ggcagaaact gacacatccg
    15661 cccctttcga ctggcataac gccacggttt actttgtgct gacagatcgt ttcgaaaacg
    15721 gcgatcccag taatgaccag agttacggac gtcataaaga cggtatggcg gaaattggca
    15781 cttttcacgg cggcgattta cgcggcctga ccaacaaact ggattacctc cagcagttgg
    15841 gcgttaatgc tttatggata agcgccccat ttgagcaaat tcacggctgg gtcggcggtg
    15901 gtacaaaagg cgatttcccg cattatgcct accacggtta ttacacacag gactggacga
    15961 atcttgatgc caatatgggc aacgaagccg atctacggac gctggttgat agcgcacatc
    16021 agcgcggtat tcgtattctc tttgatgtcg tgatgaacca caccagctat gccacgctgg
    16081 cggatatgca ggagtatcag tttggcgcgt tatatctttc tggtgacgaa gtgaaaaaaa
    16141 cgctgggtga acgctggagc gactggaaac ctgccgccgg acaaacctgg catagcttta
    16201 acgattacat taatttcagc gacaaaacag gctgggataa atggtgggga aaaaactgga
    16261 tcagaacgga tatcggcgat tacgacaatc ctggattcga cgatctcact atgtcgctgg
    16321 cctttttgcc ggatatcaaa accgaatcaa ctaccgcttc tggtctgccg gtgttctata
    16381 aaaacaaaac ggatactcac gctaaagtca tcgaaggctt tacacctcgc gattacttaa
    16441 cccactggtt aagtcagtgg gtccgcgact atgggattga tggttttcgg gtcgataccg
    16501 ccaaacatgt tgagttgccc gcctggcagc aactgaaaac cgaagccagc tccgcgcttc
    16561 gcgaatggaa aaaaactaac cccgacaaag cattagatga caaacctttc tggatgaccg
    16621 gtgaagcctg gggccacggc gtgatgcaaa gtgactacta tcgccacggc ttcgatgcga
    16681 tgatcaattt cgattatcag gagcaggcgg cgaaagcagt cgattgtctg gcgcagatgg
    16741 atacgacctg gcagcaaatg gcggagaaat tgcagggttt caacgtgttg agctacctct
    16801 cgtcgcatga tacccgtctg ttccgtgaag ggggcgacaa agcagcagag ttattactat
    16861 tagcgccagg cgcggtacaa atcttttatg gcgatgaatc ctcgcgtccg ttcggtccta
    16921 caggttctga tccgctgcaa ggtacacgtt cggatatgaa ctggcaggat gttagcggta
    16981 aatctgccgc caacgtcgcg cactggcaga aaatcagcca gttccgcgcc cgccatcccg
    17041 caattggcgc gggcaaacaa acgacacttt cgctgaagca gggctacggc tttgttcgtg
    17101 agcatggcga cgataaagtg ctggtcatct gggctgggca acagtgactt ttccggcttc
    17161 ccgttcgtca gtacctcggg aagccgccaa ccaggataaa atgtcagccc taatcagcgt
    17221 tgcgggataa agcaccgctc actcttcaac agaccgattt gcaccccagc aaatgtagcg
    17281 ttattgttac cttccttgct acagagttcg acagatatcc cgctatgaca ttctcccttt
    17341 ttggtgacaa atttacccgc cactccggca ttacgctgct gatggaagat ctgaacgacg
    17401 gtttacgcac gcctggcgcg attatgctcg gcggcggtaa tccggcgcag atcccggaaa
    17461 tgcaggacta cttccagacg ctactgaccg acatgctgga aagtggcaaa gcgactgatg
    17521 cactgtgtaa ctacgacggt ccacagggga aaacggagct actcacactg cttgccggaa
    17581 tgctgcgcga gaagttgggt tgggatatcg aaccacagaa tattgcacta acaaacggca
    17641 gccagagcgc gtttttctac ttatttaacc tgtttgccgg acgccgtgcc gatggtcggg
    17701 tcaaaaaagt gctgttcccg ctggcaccgg aatacattgg ctacgctgac gccggactgg
    17761 aagaagatct gtttgtctct gcgcgtccga atattgaact gctgccggaa ggccagttta
    17821 aataccacgt cgattttgag catctgcata ttggcgaaga aaccgggatg atttgcgtct
    17881 cccggccgac gaatccaaca ggtaatgtga ttactgacga agagttgctg aagcttgacg
    17941 cgctggcgaa tcaacacggc attccgctgg tgattgataa cgcttatggc gtcccgttcc
    18001 cgggtatcat cttcagtgaa gggcgcccgc tatggaatcc gaatatcgta ctgtgcatga
    18061 gtctttccaa gctggggctg cctggctccc gctgcggcat tatcatcgcc aatgaaaaaa
    18121 tcatcactgc catcaccaat atgaacggca ttatcagcct ggcacctggc ggtattggtc
    18181 cggcgatgat gtgtgaaatg attaagcgta acgatctgct gcgcctgtct gaaacagtca
    18241 tcaaaccgtt ttactaccag cgtgttcagg aaactatcgc catcattcgc cgctatttac
    18301 cggaaaatcg ctgcctgatt cataaaccgg aaggagccat tttcctctgg ctatggttta
    18361 aggatttgcc cattacgacc gagcagctct atcagcgcct gaaagcacgc ggcgtgctga
    18421 tggtgccggg gcacaacttc ttcccgggac tggataaacc gtggccgcat acgcatcaat
    18481 gtatgcgcat gaactacgta ccagagccgg agaaaattga ggcgggggtg aagattctgg
    18541 cggaagagat agaaagagcc tgggctgaaa gtcactaaaa attgcctgat gcgctacgct
    18601 tatcaggcct acatgatttc tgcaatatat tgaatttgga agaatttgta ggccggataa
    18661 ggcgtttacg ccgcatctgg cataaacaaa gcgcactatt ccggcgttga gaaacgccgg
    18721 aaaacgtttc aaacaggctg ctgctgtagc cgatgccgct gcactttctc gacatccatg
    18781 cactgcaacg cctttgtcgg gcaggcttca acacaggccg gccccgtctc ccgatgccag
    18841 cacagatcgc acttaatcgc cctcgccttt ttgcgcgaag aaacgacctc catcgcacca
    18901 aacgggcaag ccagcataca gcttttacag ccaatgcaac gtgtttgttc aacgaaaata
    18961 tgcccatgtt cgcggcttat cgcgtcaaca gggcagacat tcgcgcacgg tgcatcttca
    19021 cactgatgac aggctactgg cgtggtccag ctgtggtctt taatgacacg aatacgggaa
    19081 ataaactcgt ctggtgacaa cgcagcgcaa tcctgattct cctgatgcga cactgcgcaa
    19141 gccacttcac aggtacggca accgatacat ttcgtcgcat ccgcaataat aaaccggttc
    19201 atgatcattc ctttgcacta aaacagcaga gtgccagaga gcgcgtccct actgctttga
    19261 tccaacgaag gaaatgttaa agttttggca cttgtccaaa gcgttatgtg atcgcgccca
    19321 ggtcatccct gacagtaaat cccagttcat tagaaatagc ctgcgcggtt tcacgcagtg
    19381 gtttcaggag atttttctct cccacctgtt tcagacgtga tgtcgaaagc gaaatcgaca
    19441 cggcgtacgg cacccgccca tgaatatcaa acaccggaac agcaatacag gagacgccga
    19501 gttcgttttc ttctctgtcc atcgccgctc cgctttcacg aatgtgcgcc agttcgtcga
    19561 acatcgcggg cagctcggta atggtattgc gggttaacgg ctggatctca tgctgatggt
    19621 tttcccagta tgacttcacg tagtccgggt gaccaaacgc catgtagatc ttgcccattg
    19681 cggaacagta gagcggcata tgctggccaa tataggcacg ggttcgcagc atcccggttg
    19741 tgggttccag cttataaatc aaaatagcgt gatcgtcttc gcggctggag aagttaatgg
    19801 tttcaccagt ggcgatgttc agtgcctcaa gatgcggagc ggcgatatga atgatattca
    19861 gcgaagacag cgccttctgc ccgacggcaa taaatttggt agtcaggcgg taactccctg
    19921 cggcgggcgc ggtggtcaca tagccgcagg actgtaatcc ctgcaataag cgatggacgg
    19981 tactcttatt taaaccagcc agctccgaaa gatgcgccaa cggacaaccg tttggatagt
    20041 tgctcaaaat ctcaatcagc atcaacccgc gaaacagact ctggcttccg gctggacgct
    20101 ctttttcctg cgccatctcg ttctcttttt ttcccatcac ttctttcccc attttgtcgc
    20161 gtcctgatgg tagcgcaaag tgtgccgtag ttcacgatct cgacagataa tttataacca
    20221 cttgattttt atgtcttttg aaattcatca atcagattgc tttgttaaaa agtgatcgat
    20281 atatttgaaa tctagttccg catattgaaa ttttaagcca aaaaagcgat caaaaaacaa
    20341 ggaagcctag gatgaaagtg acatttgagc agttaaaagc agcctttaat cgggtcttaa
    20401 tttcacgcgg cgttgacagc gaaacggctg acgcctgtgc agagatgttc gcccgcacca
    20461 ccgaatccgg cgtttattct cacggcgtta atcgtttccc tcgtttcatt caacaactgg
    20521 aaaacggcga tatcattcct gatgcccaac ccaaacgtat aaccagcctc ggcgcaattg
    20581 aacagtggga tgcccagcgt tcgatcggca acctgacggc gaaaaagatg atggatcgcg
    20641 ccattgaact ggctgccgat cacggtattg gtctggtggc acttcgtaat gccaaccact
    20701 ggatgcgcgg cggcagctac ggctggcagg cggcggaaaa aggctatatt ggcatttgct
    20761 ggaccaactc catcgccgta atgccgccgt ggggcgcaaa agagtgtcgc atcggcacta
    20821 acccgctgat cgtcgccatt ccttccacgc cgatcaccat ggtcgatatg tcgatgtcga
    20881 tgttctctta cggcatgtta gaagttaacc gcctggcagg ccgtcagctc ccggtcgatg
    20941 gtggctttga tgatgagggc aatttgacca aagaacctgg cgttatcgag aagaatcgcc
    21001 gcattttgcc gatgggctac tggaaaggtt ctggcatgtc gattgtgctg gatatgatcg
    21061 ctactctcct ttccgacggc gcatcggttg ccgaagtcac ccaggacaac agcgacgaat
    21121 acggcatttc acaaattttt attgccattg aagtggacaa gcttatcgac ggtcccaccc
    21181 gcgatgccaa gctgcaacgt atcatggatt acgttactac cgctgaacgc gctgacgaaa
    21241 accaggccat ccgcttaccc ggccacgaat ttactaccct gctggccgaa aaccgccgta
    21301 acggcatcac tgttgatgac agcgtgtggg ccaaaatcca ggcgttatga ggagataagt
    21361 catgattttt ggacatatcg cgcagcccaa tccgtgccgt ttgcccgccg ccattgaaaa
    21421 ggcgctcgat tttctgcgcg ctaccaactt caacgtcctg gagccgggcg ttgtcgaaat
    21481 cgacggcaag aatatttatg cgc
//
LOCUS       DAJTSA010000066        21275 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000066 DAJTSA010000000
VERSION     DAJTSA010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.066"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..444)
                     /locus_tag="OO369_002642"
     CDS             complement(<1..444)
                     /locus_tag="OO369_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR5982870.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
     gene            complement(499..1350)
                     /gene="manZ"
                     /locus_tag="OO369_002643"
     CDS             complement(499..1350)
                     /gene="manZ"
                     /locus_tag="OO369_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR5982871.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1363..2163)
                     /gene="manY"
                     /locus_tag="OO369_002644"
     CDS             complement(1363..2163)
                     /gene="manY"
                     /locus_tag="OO369_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR5982872.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2226..3197)
                     /gene="manX"
                     /locus_tag="OO369_002645"
     CDS             complement(2226..3197)
                     /gene="manX"
                     /locus_tag="OO369_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR5982873.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3660..5210
                     /gene="yoaE"
                     /locus_tag="OO369_002646"
     CDS             3660..5210
                     /gene="yoaE"
                     /locus_tag="OO369_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR5982874.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5214..6812)
                     /locus_tag="OO369_002647"
     CDS             complement(5214..6812)
                     /locus_tag="OO369_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5982875.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="OO369_002648"
     CDS             complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="OO369_002648"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5982876.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFSHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8491..9069)
                     /locus_tag="OO369_002649"
     CDS             complement(8491..9069)
                     /locus_tag="OO369_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR5982877.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="OO369_002650"
     CDS             complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="OO369_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR5982878.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10508..10687
                     /locus_tag="OO369_002651"
     CDS             10508..10687
                     /locus_tag="OO369_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR5982879.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10807..11166)
                     /locus_tag="OO369_002652"
     CDS             complement(10807..11166)
                     /locus_tag="OO369_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR5982880.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11528..11872)
                     /locus_tag="OO369_002653"
     CDS             complement(11528..11872)
                     /locus_tag="OO369_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5982881.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12004..13914
                     /locus_tag="OO369_002654"
     CDS             12004..13914
                     /locus_tag="OO369_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR5982882.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13972..14667
                     /gene="tsaB"
                     /locus_tag="OO369_002655"
     CDS             13972..14667
                     /gene="tsaB"
                     /locus_tag="OO369_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR5982883.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14707..15288
                     /gene="yeaY"
                     /locus_tag="OO369_002656"
     CDS             14707..15288
                     /gene="yeaY"
                     /locus_tag="OO369_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR5982884.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15494..17179
                     /gene="fadD"
                     /locus_tag="OO369_002657"
     CDS             15494..17179
                     /gene="fadD"
                     /locus_tag="OO369_002657"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR5982885.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17261..18376
                     /gene="rnd"
                     /locus_tag="OO369_002658"
     CDS             17261..18376
                     /gene="rnd"
                     /locus_tag="OO369_002658"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR5982886.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18430..19395)
                     /gene="yeaX"
                     /locus_tag="OO369_002659"
     CDS             complement(18430..19395)
                     /gene="yeaX"
                     /locus_tag="OO369_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR5982887.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19451..20068)
                     /gene="yeaW"
                     /locus_tag="OO369_002660"
                     /pseudo
     CDS             complement(19451..20068)
                     /gene="yeaW"
                     /locus_tag="OO369_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(20410..20976)
                     /locus_tag="OO369_002661"
     CDS             complement(20410..20976)
                     /locus_tag="OO369_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5982888.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21188..>21275
                     /locus_tag="OO369_002662"
     CDS             21188..>21275
                     /locus_tag="OO369_002662"
                     /inference="COORDINATES: protein motif:HMM:NF025073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5982889.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         5264 a         5273 c         5467 g         5271 t
ORIGIN      
        1 gagaagctta taaatttttt ccagatcgtc gatatttcga acgcggatta acagccgacg
       61 ctgttctaat tgcatcacca acacgccatc ttccgacaag ttcatcgctt tgattcggct
      121 atattcaatc cagacattgg cgaagaaaaa acctttttgt ttaaagatga tcttcggaat
      181 gcggatccag aatatataaa aacccatcag agccagtgca cttaataacc atgttgttat
      241 tagcgcgcca tgattcgtga cgttgttata gataagaatg acaatcaatc cgacgaagat
      301 aacgctatcg atgcgaccac gccggagcaa aggaattgcc agcagggtgg ggccgttacg
      361 gcggggcatg atgaactgat cgtagatcgc gaaggccagg agtgcggcga tgaaaagaat
      421 cagtaccagg tccgtgattg tcattaatcc tccagataaa aaaacggggc caaaaggccc
      481 cggtagtgta cgacagtctt acagtcccag caggccgcaa gcgtaaccag cgataccgat
      541 gacgaagaag ccaacgatga tccacagcgg gttaactttt ttgcgcagca gccacataca
      601 agcaaatgtc agcagcagtg gtacaaggcc tggcattaac tggtcaagaa tagtttgcac
      661 ggtggtgaca cgggtctgtc catcctgccc ggtgatggtc gaaaccacca gcgggatgtt
      721 gacatgtgtc cacttgttaa ccaatgcccc catgacaaac aggccgagga tagacgcccc
      781 ttccgtcagt ttttgcagga agccaccacc catatcttta acgatatcga tacctttgga
      841 gtaaccatac gctacgccgt agtaacgggt tgccagacgc accaggttaa acaggatgaa
      901 gaacagcagc ggacctaaca ggctgccgct catcgcgata ccggcaccca gtgctgcaaa
      961 taccggacgt acggttcccc agaagatcgg gtcgcctaca ccagccagcg gccccatcaa
     1021 accgactttg ataccgttga tagcaccgtc atcgatctct gcgccattag cacgctgttc
     1081 ttccagcgcc agggttacgc cgagaatcgg tgcagccacg aacggctggg tgttaaagaa
     1141 ctccaggtga cggcgaatag cttgtttacg agcttcgttg ttctcagggt agaggcgacg
     1201 aattgccggt accatagaga agcagaaacc cagtgcctgc atacgttcga agttccatga
     1261 accctggaag aggttagaac gcaggaatac gccacgaata tcactttgag tgagtttttt
     1321 ctcggtggta gtttgagttg tatcaaccat ttcgctcacc tgttagtcca gttcgttatc
     1381 gagatcgttg ttaccagctg cctgagcagg cgcaccggct acgcggttgt atttcgggct
     1441 aagttggatg tagagcactg ccataacagt accaatcaca cccagagcaa ccaggttaaa
     1501 gttggtgaat gctgcggtta cgaagccgag gtagaagaac ggcatcaggt agccagcacg
     1561 catcatgttg ataaccatcg cataaccaac caccacgatc atgcccccgg cgatattcag
     1621 accattggtc accacttccg gaatcgcatt cagcatgttc tgtacttcgc tggtaccaac
     1681 agacagcgca acgatgacgg ccggaatagc cacacgcatt gcttgcagga acagagaaga
     1741 aacgtggatc caggaaatcg ctgtcaggtt gccgttatca gcagccttat ccgcagcgtg
     1801 ctggaaagca acggtaatag tacgaacgat gatggtcagt acctggcccg cagcggccag
     1861 agggattgcc agtgcgatac ctgcaccaat gctctgatga cctgcgataa ccagaatggt
     1921 agaaatgata gaagccagag cggcgtcagg cgcaactgca gcaccgatgt tcatccagcc
     1981 cagcgcgatc atttccagcg taccaccgat aataataccg gttttcatat ccccaagaac
     2041 gatacccacc agggtacacg cgattagcgg acggtgaaac tgaaattcat cgaggattga
     2101 tcccatacct gcgatacagg ctacgataaa taccagcaca atttgaagag tggtaatctc
     2161 cattgtactt ctcctgttta cgtaagactt aagtgtgaaa actgaatgat aatcaacaca
     2221 atacgttact tatcgatttt gctgatcaga tccatcattt tcagtttcgg atcggtggaa
     2281 accttacgga cttccagctc aataccgcgt gcattcagtt ttttgaacgc ctcgatatct
     2341 ttttcatcaa ccgaaaccgc gttattcacc tgggttttac cctgacggaa tgccatacca
     2401 ccgacgttaa cagaggtgat tttcacgccg ccttcaacga gacgctctac atctgttggg
     2461 ttggtaaata acagcattac gcgttcgcca gcatatttcg ggttgttgta gacgcgaatc
     2521 attttggcaa catcaactac gtgtgctgtt acgcccggag gtgcaacctg ggtgagcagt
     2581 gtcttacgaa cggtatccgc agccacttca tcactaacaa caataatacg ggagacattg
     2641 gtttctttgg tccagcgggt ggcgacctga ccgtgaatca gacggtcgtc gatacgcgca
     2701 aggccaataa ccatgtagtc gtttggcccc attggttttg ccggagttgg agccgctttt
     2761 ggtgctgcgg cagcgggtgc tggcgcggct ttttcaaccg gtttggcttt cagtgctttc
     2821 acgccttcac ggcctgtttc tactgccagc gcaaccagtt catcaaagct cgggtcatca
     2881 tcacgggcca ttaacgtttc cacgagcatt ggaatgttaa cgcctgcaat gacttcataa
     2941 tgctctttgt cgacgacaat gcggctggca gcattgaacg ggctgcctcc ccatgtatca
     3001 acgagaaaca gcacgccttt agtggtgtcg agttttgcca actgagcgtt gtatttttca
     3061 atcagcgttt cggcattttc acctggaacg aaatcgatcc agccgacgtt ttcctgctcg
     3121 cctaacagca tttctgccgt tttaagcaac tgctctgcag cccaaccatg tgtgcctata
     3181 acaatagcaa tggtcacttg ctacctcctt tattatcgtt aacacctcaa cgtgccagat
     3241 gtatttttga atcgtcgtcc acaatcgaat cgattcagat aagggctaca accaaaaacc
     3301 cctgcgtttc gcgatttatt ttagatatcg aaaaaattat tttatgtgat gaagatccgt
     3361 aatttaactt tcgattatca gaaatttcga aaggtaaaat atccttgtca cattcgtttg
     3421 caaaggaagg taaatctttg ccaaatcaga ggcgtctctg atatgtttaa ctcccgttta
     3481 acaaccatgg agtatagggc agtagcccgc agtatggatc gtcaccgacg tcatttcagc
     3541 atcaggcctt ttaacgcctg cctttctggc actctatgcc gcacctttcg tttgcatttt
     3601 gtcgttacgc ctgcattatt tctggcgtcg aatagctatt ccttaagcag gagcttgtca
     3661 tggaattctt aatggacccc tcaatttggg cggggctact cacgcttgtt gttctcgaaa
     3721 ttgtgctggg tatcgataac ctggtcttca tcgccattct tgctgacaaa ctgccgccaa
     3781 aacaacgcga taaagcgcgt ttgctggggt tatcactggc gctgattatg cgtctggggc
     3841 tgctgtcgct gatttcatgg atggtcacgc tgaccaaacc gctatttacc gtcatggatt
     3901 tctccttctc cggacgcgat ctgattatgt tgttcggggg gatattcttg ctgttcaaag
     3961 caacaaccga actgcatgaa cggctggaaa accgcgatca tgattccggc cacggtaaag
     4021 gctacgccag tttctgggtg gtcgtcacac agattgtcat ccttgacgcc gtcttctcgt
     4081 tggatgcggt aattactgca gtagggatgg ttaaccatct gccggtgatg atggcggcgg
     4141 tagtgattgc gatggcggtt atgttgctgg catcgaaacc gctgacgcga ttcgttaacc
     4201 agcaccccac ggtggtggtg ctctgtctga gcttcctgtt aatgattggt ctgagtctgg
     4261 tggcagaagg tttcggtttc cacattccga aaggttacct gtatgccgcg attggcttct
     4321 cgatcattat cgaagtgttt aaccagattg cgcgtcgcaa ctttattcgt caccagtcga
     4381 ctttgccgct gcgagcgcgt actgccgatg ccatcctgcg tttgatgggc gggaaacgtc
     4441 aggccaatgt tcagcacgat gccgataacc cgatgccgat cccggaaggt gcatttgccg
     4501 aagaagaacg gtacatgatt aacggcgtac tgacgctggc gtcgcgttct ctgcgcggga
     4561 tcatgacgcc gcgcggtgaa ataagctggg ttgacgctaa tctcggggtc gatgaaatcc
     4621 gcgagcaact gctctcttca ccgcacagtc tgttcccggt atgtcgcggt gaactggatg
     4681 aaatcatcgg tatcgtacgt gctaaagaac tgctggtggc gctggaagag ggcgttgatg
     4741 tggcggcgat tgcttcggcg tctccggcga ttatcgtccc ggaaaccctc gatccgatca
     4801 acctgctggg cgtgctgcgt cgcgctcgcg ggagctttgt tatcgtgacc aacgagtttg
     4861 gtgtggtaca aggcttggtc acgccgctgg atgtgctgga agccattgcg ggtgaattcc
     4921 cggacgctga cgaaacgccg gaaatcatta ccgatggtaa cggctggctg gtaaaaggcg
     4981 gtacagattt gcatgccttg cagcaggcgc ttgatgttga gcaccttgcc gatgacgatg
     5041 atatcgcgac ggtcgcgggc ctcgtgatct cggcaaatgg tcacattccc cgtgtgggcg
     5101 atgtgattga tgtagggcca ctgcatatca ccatctttga agccaatgat tatcgtgttg
     5161 atctggttcg cattgttaaa gagcaaccgg cgcacgatga agatgagtaa gcgttaacgt
     5221 aacgacataa tgggcgtgat atgtccatta tgccgggcgg gcggcggttg gctgcccgcc
     5281 agccatttgg gaaaatcacg tagcggcatc ggctgtgcgt acaaataccc ttgcaaaata
     5341 tgcacgccat gacggcgcaa atacttcgcc tgttcttgcg tttccacgcc ctccgccacc
     5401 agttcaatat tcagtctttg ccccagcgcg atgatgatat cggtcaccgt cgagttaacc
     5461 gcgtcagaac ctatagctgc ggtaaatgac ttatcaattt tcagcacgtc aggacgtaat
     5521 gtttcaagcc aggaaaacga gctgttgccg gtgccgaagt catcaatcgc cagtttgacg
     5581 tttttacgat gcagctcgcg ggcaatccga taatcaacat ccagtaaggc atcgcgttcg
     5641 gtgatttcga ggatcagttg ctgaatcggg tgagcgctaa accagtactg attgagatct
     5701 tttatcagca caccacggcg aaaatggctg ggtgcgacgt taatgccaac atgaaactga
     5761 ctactcatcg ggaaaacatg gtgctgacga atggtttctg ccatcacata gtgggtcagt
     5821 ggcacaatta aatgatgttc ttccgcgata ggaataaaca catccggtga aatccagccc
     5881 tgacgcggat tattccagcg cagcaaaatc tctacaccaa tacattgctg gctgcgcgca
     5941 ttaagcaaag gctgacagaa caattcgaac tcatgttgcg ccaggcccag attaatttcg
     6001 cgggaaaagc tcatccggta agcggtcgcc agccaggcga tatagcccac cagtaaactt
     6061 agcagcaccg ccagcggtaa ttgtgttgga agatagtgcc atgccagcgc cgtagccccc
     6121 ggaccattta cgttaatggt aaaaggaaag ctttgcgaag aaacctgata gttttcattg
     6181 tcttcaggtt gcggaaggga atctaccagc ccattaccat agagcaaatg ccgtttgtcc
     6241 accgtcaggc tggcgctact gatttgcggc agttgtggct caaggagcat tgccgtcagt
     6301 aagtcgatgt taatcatctc tatgacccca gcattgctac tgcccgctgc aggcgtccat
     6361 tgaatcaaaa ccggactgcc tttaatcagg gcacggtcga tcgtcaggcg taaaagtggt
     6421 tgcggtgcag gaagttcagc cagaatgtcc acgacgggca cattgcgata accaaaaatg
     6481 ctggagcaat aaagtgtgcc gtcttgcacc aggccaatgg atcgcacagt ttggagtttt
     6541 gccgcctgtt tacgtaatgg caaatgggcg acagagcagg gcagaccaat cagcggaagc
     6601 aagactttgc tacctgcctg taggggaagc agtactttat ctaattcctc tacagcgtga
     6661 ttggcgaatt gtactacccg ttgttgattt aagttacgct gtgaaataaa tcgcacgctc
     6721 agggtcgaag cgagcgtaac gagtgcgcaa atcgtacaaa caatcattcg attacggcga
     6781 taggttttaa tgatccgttg tgctttttgc atgcgggcca ccctgtaagc gtatggcgac
     6841 aaacgcctga cgcaacagtg gaagtgtagt ggggaatatt acagcagacg agaaagcggg
     6901 tataaattcg cccatccgtt gcagatgggc gagtaagaag tattagtcac actggacttt
     6961 gattgccaga ccaccgcgtg aggtttcgcg gtatttggcg ttcatgtcct taccggtttc
     7021 gtacatcgtt tcgatgacct tatccagcga gacgcgcggt gcactggtgc ggcgcagagc
     7081 catccgtgcg gcgttaatcg ccttcacaga ggcaatggca ttacgctcaa tgcacggcac
     7141 ctgaacctgc cctgcaaccg ggtcgcaggt taaaccaagg ttgtgttcca tgccaatttc
     7201 cgccgccacg caaacctgtt ccgggctacc gcccagcagt tctgcaagac ccgcagcagc
     7261 cattgaacag gcaacaccca cttcgccctg gcaaccaact tccgcaccgg aaatagaggc
     7321 gttcatttta tacaatgcac caatcgcgcc cgctgccata aagtaacggg tatagatgtc
     7381 tgggctgacc gattcaataa agtggtcata gtaagccagc actgccggaa cgataccgca
     7441 ggcaccgttg gttggcgcag ttaccacacg accaccggcg gcgttttctt cgttaactgc
     7501 cagcgcaaac atgtttaccc agtcaatgac attcatcgga tcgttagaca gtttatcgct
     7561 ggaaactaac atccggcgca gggcagacgc acgacgtggc acgcgcagcg ggcctggcag
     7621 tacaccttcg gtgttcatcc cgcgatcgat acatgcctgc atggtttgcc agacatgcga
     7681 gaaatactcg tcgatctctt tcttgctgtg cagcgccagt tcgttctgca tagcgagacc
     7741 agacagcgaa tagccggttt cattacagta cgcgagcagt tcggtggcag atttgaacgg
     7801 atacggcacg ctcacttcgt tggcagcatc ctgaccaaag tgttcttcat cgacgataaa
     7861 accgccgccg atggaataat aagttttgct gtagacgact tcatcgccgt tataggcgtg
     7921 gatttgcata ccgttttcat gcagcggcag gttgccgtta tgaaaacgca tcccgttgtc
     7981 gcgcgggaaa tccacttcat gccgtccctg tgccagcagc agacgttcgc gctcttttac
     8041 gtcgcgaata aaaccgggaa tactgtcgat atccacggtg gcaggttcgt tacctgcaag
     8101 acccataata atggcgatat cggtgtggtg gcctttaccc gtcagcgaca gtgaaccata
     8161 aacgtccacg gcaacgcgag taacgctatc cagtaagcct ttttcgacca gatcatcgac
     8221 gaactgttta cccgccttca taggccctac ggtatgggaa gatgagggac caatccccac
     8281 cttaaacatg tcgaatagac taatcacgat aatactcctg acaaggtgac tggacttcca
     8341 gtaacgaact aataacagcg catagtgtaa gagggaacgc ggcgactggc ttaactattc
     8401 acatgaatta aactaatcaa aaaccgcgat ttaactaata ttgcgatgat tgtctcaagt
     8461 agtgttttgt agatgtaaag ataagtatag tcagggggtc acaccaattt gcagcgccag
     8521 ctcacgaatt atgcctgcgg tcattcccca tacaaaatac tgttcgtacc aggacagcca
     8581 tacccgatgt gaatcgccac ggcggtagat atctaaaggg tgataacgac ccagatgtaa
     8641 tgcctgggcg agcggcattt caaacaccgc cgagacttca tcttcactgg cgcgatacgg
     8701 cagatcgggc gggataatgc cgaccactgg ggttacttga tagccagtta cgctatcgac
     8761 gggcggcagc acgccgataa cttcaacggc ggaaggcggt atagcgacct cttcttcagc
     8821 ttcgcgcagc gcggcggcga taactgatgc gtccgtgtca tcgactgcac ctccagggaa
     8881 tgccacttgt ccagcgtgtt tacgcagatg aattgaacgc tgagtcagca acaaccccgg
     8941 ttgcggtcga cggacgatgg ggattaacac ggcagcctga cgatgattta ggggttcccg
     9001 gttaatttgc gggcgcaaaa gttgaaagcg agataaaaaa tcatcaagcg tcaggctacg
     9061 gtattccacg tcttacttct ccagttggcg taatatctta ttaactttat caaaagtttc
     9121 ctgatattcc gcttcttcct ggctatcggc gacaattcca ccgcccgcag agcagtatat
     9181 ttgtccgtta atggcagtca gcgtgcggat agtaatgctg gtatccatgt tgccgcaaaa
     9241 gctcaaatag ccaatgctgc cgcaccaggc attacgtcgc tgcggttcca gttcgtcgat
     9301 aatttccata gcccgtactt tcggagcccc ggttattgag ccaccaggaa aagctgcgcg
     9361 cagcagatcg ctggcgtgta actgttctgg tagtcgcgcc gttatagtgc tgaccagatg
     9421 atgcacggca gggaagggtt ccaccacgaa gagctctggt acttttaccg aaccggctac
     9481 ggcaacacga ccgatatcat tacgcattaa atcgacaatc atcagatttt cggcacgatc
     9541 tttcgctgag ttcgccagtt tttctgcttg tttgctatct tcctgaggat cgggcaggcg
     9601 tggtagcgtg cctttaatcg ggcgagtctg gatttcacta ttatcacaaa gaataaaccg
     9661 ctctggcgaa aggcttaaaa ttgcaccctg ttcaagacgt aaaaaagcgc taaatggcgc
     9721 gcggttggcc tgattaagct gaaggaatgc ctgccattca tcgccagaat aggtcgcatg
     9781 aaaacgctgg gcgagattca cctgatagca atcaccgctg tgcagatatt cctgtacctg
     9841 gcgaaatttt tcgccgtact gctcgtgggt catattggat tgccagtcgg aagtgagcgt
     9901 gaaatcttcc tgcggcgaga attgctggct ttccagccag gcccgacggg cattgacatc
     9961 attatgactc agcaaagaaa ctgtatgacg ctggtggtcg acaatgagcg cccaatcgta
    10021 gatacccact gccatatccg gcagaacgat atcttgttcc gcaatttctg gcagtgactc
    10081 aaaacggcgg cccagatcgt agccaaacaa ccccagtgcg ccgccctgaa atggcaaatc
    10141 ttcgttatgc gttgggcgaa tgtctgcgcg atccagcacc tgctggagca cctgtagcgg
    10201 gtcatcagtg gtcgttgtgc gtttttcgct ttcactaaca acggtttctt tacctaaagt
    10261 ggttaaagtg caaatcggat cggcgaccac aatatcaaag cggctatacg gatgatcggc
    10321 atagccggag tgtaaaagca tcgcccacgg caggtggctt aagcgggaga aataaaattc
    10381 agcggcgtcc tgacgccagg gtaaagtaat cacagtggga gataacgtct tcatcagtcc
    10441 tgactctact ggctatgtgc cgggtagcat gaaataatta gcgccaacaa tttagcagga
    10501 gttaacaatg tttgcaggtt taccttcact catccatgaa cagcagcaaa aagctgtcga
    10561 gcggatccag gaactgatgg cacaagggat gagtagcggc caggcaatcg cgctggtggc
    10621 agaagagctg cgtgccaacc actccggcga gctaatcgtg gcgcgttttg aggatgaaga
    10681 cgagtaacac actaatccgt atcgatttcg ttatagaagc cgatgatcat ttgattgtga
    10741 gaaaaaggac gctttttata tgtgattaaa agcgtcctga cccatttatg ctgatataaa
    10801 tttaactcag accagtgctt ttagttcttc ctgcatatat gttgaaaagt attcaggatt
    10861 tttaatcaca gagcgttcac ctgtctccat tttttttgcc catccaacca ttttttccgt
    10921 gaacccaggg ttccagcctt cctggtcagc tcgcgccgta atatcagcgg cactcgcggg
    10981 tactcccagt tcttttaaat atttaaagat tcccttcgcc gtgctttcat tcatcgaact
    11041 tggtgttggc gctgatacat ccatggcacc aatgaaatcc agggctttat ctattacagc
    11101 aggcatattc ttatccttaa aattaaccat gttatcgata aggctatata tgaccttaat
    11161 ttgcatctaa tatcaaaggg attataacga tatggcacga gttaacctgt tgacattcag
    11221 gtgttttaaa ggtagcagct catggtgtgt tatccagtaa gatggagaaa attaacggtc
    11281 agcccgcacg ctataaccag attgttcagc taagaaagaa aaccagagtt gctaatcaaa
    11341 gggcgaacac aacggcatgg ttatgtttat ttatatagtt agttttatag ataaaattga
    11401 aattataaat ataaataatg aattagcaga agcatcaaca gtaatatctt attctctggt
    11461 cgatttatgt aaaaccgacc agtattatcg ataaattatc gtctgactta ttttgcttcg
    11521 ataaagctta caccgcagcc acaatcttaa tttcaacttt atacttcggg ttcatcaaac
    11581 ccgcttgtac cgtgcagcgc accggcgcat gacccgcgac aacccaggca tcccacgctt
    11641 tattcatcgc cgcgaagtcg tttttatcgg ccaggaaaat ggtggcatcc agaatgctcg
    11701 atttattgct gccctgtttt tccagcacgg cgtcaatctg tgccagcgtg ttggcggttt
    11761 gctcaaaggc atcggcgtcg aggttttccg gtacaccagt gtagtagagc gtgttgttgt
    11821 ggattactac atcagaccag cgggcttcag catcgatacg aacgatagtc atcattttgt
    11881 cctcattata cttccatttt cgtgctgcaa agactgccac aatgtgaggt tgccgtcacc
    11941 acttcaactg gcgaaagcgc cctcatcctg acataatccc tgttcaaatc aacagggggt
    12001 agtgtgacgg acgattttgc accagacggt cagctggcga aagcgatacc aggctttaag
    12061 ccgcgagaac cacagcgaca gatggcggta gccgtcaccc aggcgataga aaaaggccag
    12121 ccgctggtgg tggaagcagg aaccggtacg ggcaaaacct acgcttacct ggctcctgcg
    12181 ctgcgggcga aaaagaaagt cattatctcg accggctcaa aagcgttgca ggatcagctc
    12241 tacagccgcg atttgccaac agtctcaaag gcattgaaat atacgggcaa cgtggcgctg
    12301 ctgaaagggc gctcaaacta cctctgcctc gaacgtctcg aacagcaggc gctggcgggg
    12361 ggcgatctgc cggtacaaat cttaagcgat gtgatcctgc tgcgctcctg gtctaatcaa
    12421 acagtcgatg gtgatatcag cacctgcgtc agcgtggcgg aagattcaca ggcgtggccg
    12481 ctggtcacca gcaccaacga caactgtctt ggcagcgact gcccgatgta taaagattgc
    12541 tttgtggtca aagcacgtaa aaaagcgatg gacgccgatg tggtggtggt aaaccatcat
    12601 ctctttctgg cggatatggt ggttaaagag agtggatttg gcgagttgat ccctgaagcg
    12661 gacgtcatga tcttcgacga agcccaccag ctaccggaca ttgccagcca gtattttggt
    12721 cagtcactct ccagtcgaca actgctcgac ctggcaaaag acatcaccat cgcctaccgc
    12781 accgaattaa aagacaccca gcagttacaa aagtgcgctg atcgtcttgc ccagagtgcg
    12841 caggattttc gtctgcaact cggtgagcca ggttatcgcg gtaacctgcg tgagctgtta
    12901 gctaatccgc aaattcagcg ggcattttta ctgctcgatg acaccttgga actttgttat
    12961 gatgtggcga aactgtcact ggggcgttcc gccttgctgg atgcggcatt tgagcgcgcc
    13021 acgttgtatc gcacacggct gaagcggcta aaagagatca atcagccggg ctacagctac
    13081 tggtacgaat gcacttcgcg ccattttact ctggctctca cgccgctcag cgtggcggat
    13141 aaattcaaag agttaatggc gcaaaagccc ggtagctgga tcttcacctc agcaacgctg
    13201 tcggtgaacg acgatctgca tcatttcacc tcgcggcttg ggatagaaca ggcggagtcg
    13261 ttgctattgc caagcccgtt tgattacagc cgccaggcgt tactctgtgt gccgcgcaat
    13321 ctgccgcaaa ccaaccagcc aggttctgct cgccagttag cggcaatgct gcgaccgatc
    13381 atcgaagcta acaacggtcg ttgttttatg ctttgtacct cgcacgccat gatgcgcgat
    13441 ctggccgagc agttccgcgc taccatgacg cttcccgttt tgttgcaggg ggaaaccagt
    13501 aaaggccaac tgttgcagca gtttgtcagt gccggtaatg cgcttcttgt ggcaaccagt
    13561 agtttctggg aaggggtgga cgtgcgtggc gatacattgt cattggtaat tatcgacaaa
    13621 ttgccgttta cctcgccgga tgatccactg ttaaaagcgc gcatggaaga ttgtcgtttg
    13681 cgcggtggcg acccgttcga tgaagtgcaa ctaccagatg ccgtcattac tctcaaacag
    13741 ggggtaggtc gactgattcg cgacgccgac gatcgtggcg tgctggtgat ttgtgactat
    13801 cggctggtga tgcgtcctta cggcgcgacg tttctcgcca gtctgccgcc cgcgccacgc
    13861 acccgtgaca ttgcccgtgc ggttcgtttc cttgcgatac catcctccag gtaatttgtt
    13921 acggagtatg gtatgatgcg cgccttttta ttgatctaag cacgaagacc catgcgaatt
    13981 ctggctatcg ataccgcgac agaggcctgc tctgtcgccc tgtggaacga cggtactgtc
    14041 aacgctcatt ttgagctttg ccctcgtgaa catactcaac gaatcttacc gatggtgcag
    14101 gatatcctga ccaccagcgg aacttccctg actgatatta acgctctggc ttacgggcgc
    14161 ggccccggta gctttaccgg cgtgcgcatt ggtattggca tcgcgcaagg gctggcgctt
    14221 ggcgcggaat taccgatgat tggcgtctcc acgctaatga cgatggcgca aggggcgtgg
    14281 cgcaaaaacg gcgcaacacg cgtgctgtca gccattgatg cgcgaatggg cgaagtttac
    14341 tgggccgaat atcagcgtga tgaaaacggt atctggcacg gtgaagaaac cgaagccgta
    14401 ctcaaacccg aactcgttca tgaacgaatg caacagcttt ccggcgaatg ggtgacggta
    14461 gggacgggct ggcaagcctg gccggatctc ggtaaagaga gcgggctggt tttgcgcgat
    14521 ggcgaagtgt tactccccgc tgcagaagat atgctgccga ttgcttgtca gatgtttgcc
    14581 gagggtaaaa cggtggcggt ggaacatgcc gagccggttt atttgcgtaa caacgtcgca
    14641 tggaagaaac ttccgggcaa agaatgaatc ttaagagtat gccctgagaa aaaaaggagt
    14701 cgcatcatgg cggttcaaaa gaatgttatc aaaggcatac tggcaggtac gtttgcgcta
    14761 atgctgagcg gttgtgtcac tgtgccggac gccattaaag gcagcagtac cacgccgcaa
    14821 caagatttag ttcgggtgat gagtgcgccg cagctgtacg ttggtcagga ggcacgcttt
    14881 ggtggcaaag tggttgcggt acaaaaccag caagggaaaa cccgcctgga aattgctacc
    14941 gtaccgctgg acagcggagc taggccgacg ctgggagaac cttctcgcgg tcgcatttat
    15001 gccgatgtga acggttttct ggacccggtg gatttccgtg gacaactggt tacggtagtc
    15061 gggccaatca ccggtgcggt tgacggcaaa atcggcaata cgccctataa atttatggtg
    15121 atgcaagtaa cgggttacaa acgttggcat ttaacccagc aggtgattat gccgcctcag
    15181 ccgattgatc catggtttta tggcggtcgt ggctggccct atggctacgg cggatggggc
    15241 tggtataatc ccggccccgc gagagtacaa acagttgtaa ccgaataatt gcttgttttt
    15301 aaagaatgca gaaacagcgg ctggtccgct gtttctgcat tcttacggta aagataaaaa
    15361 taaatagtga cgcgcttcgc aaccttttcg ttgggtaatt atcaagctgg tatgatgagt
    15421 taatattatg ttaacggcgt gtatatcatt tggggttgcg atgacgacga acacgcattt
    15481 tagaggtgaa gaattgaaga aggtttggct taaccgttat cccgcggacg ttccgacgga
    15541 gatcaaccct gaccgttatc aatctctggt agatatgttt gagcagtcgg tcgcgcgcta
    15601 cgccgatcag cctgcgtttg tgaatatggg ggaggtgatg accttccgca agctggaaga
    15661 acgcagtcgc gcgtttgccg cttatttgca acaagggttg gggctgaaga aaggcgatcg
    15721 cgttgcgttg atgatgccta acttattgca atatccggtg gcgctatttg gcattttgcg
    15781 tgccgggata atcgtcgtaa acgttaaccc gttgtatacc ccgcgtgagc ttgagcatca
    15841 gcttaacgat agcggcgcat cggcgattgt tatcgtgtct aactttgccc acactctgga
    15901 aaaagtggtt gataaaaccg ccgttcagca cgtaattctg acccgtatgg gcgatcagct
    15961 atctacggca aaaggcacgg tagtcaattt cgttgttaaa tacatcaagc gcttggtgcc
    16021 gaaataccat ctgccagatg ccatttcatt tcgtagcgca ctgcataacg gctaccggat
    16081 gcagtacgtc aaacccgaac tggtgccgga agatttagcc ttcctgcaat acaccggcgg
    16141 caccacaggt gtggcgaaag gcgcgatgct gactcaccgc aatatgctgg cgaacctgga
    16201 acaggtcaac gccacctatg ggccgttgct gcatccaggt aaagaactgg tagtaacggc
    16261 actgccgctg tatcacattt ttgccctgac cattaactgc ctgctgttta tcgaactggg
    16321 tgggcagaac ctgcttatca ctaacccgcg tgatattcca gggttggtaa aagagttagc
    16381 gaaatatccg tttaccgcta tcactggcgt taacaccttg ttcaatgcgt tgctgaacaa
    16441 taaagagttc cagcagctgg atttctccag tctgcatctt tccgcaggcg gtgggatgcc
    16501 tgttcagcaa gtggtggcag aacgctgggt gaaactgacc ggacagtatc tgctggaagg
    16561 ttatggcctg accgagtgcg cgccgctggt gagcgttaac ccgtatgata ttgattatca
    16621 tagcggtagc atcggtttgc cggtgccatc gacggaagcc aaactggtgg atgacgacga
    16681 caacgaagta ccaccgggtc aaccgggtga gctttgtgtc aaaggaccgc aggtgatgct
    16741 gggttactgg cagcgtccgg atgctacaga tgagatcatc aaaaatggct ggttacacac
    16801 cggcgacatc gcggtgatgg atgaagaagg attcctgcgc attgtcgatc gtaaaaaaga
    16861 catgattttg gtttccggtt ttaacgtcta tcccaacgag attgaagatg tcgtcatgca
    16921 gcatcctggc gtacaggaag tcgcggctgt tggcgtacct tccggctcca gtggtgaagc
    16981 ggtgaaaatc ttcgtagtga aaaaagatcc atcgcttacc gaagagtcac tggtgacttt
    17041 ttgccgccgt cagctcacgg gatacaaagt accgaagctg gtggagtttc gtgatgagtt
    17101 accgaaatct aacgtcggaa aaattttgcg acgagaatta cgtgacgaag cgcgcggcaa
    17161 agtggacaat aaagcctgag cgttaagtca gtcgtcagac gccggttaat ccggcgtttt
    17221 ttttgacgcc cactaaagag aaaacaattt gaattaccaa atgattacca cggacgatgc
    17281 gctggcttct ttgtgtgaag ccgtccgtgt ctttccggcg atagccctgg atactgaatt
    17341 tgttcgtacg cgcacttatt acccgcagct ggggttgatt caacttttcg atggcgagca
    17401 tctggcgcta attgatcctc tcgggatcac cgactggtca ccgctgaaag cgatcctgcg
    17461 cgatccgtcc atcacaaaat ttctccatgc aggcagtgaa gatctggaag tgttcctcaa
    17521 tgtctttggc gaattaccac aacccttgat tgacacgcaa atccttgctg ccttctgcgg
    17581 acgcccgatg tcatggggtt tcgcttccat ggtggaagag tattccggcg ttacgctgga
    17641 caagagtgaa tcgcgcaccg actggctggc cagaccgctg accgaacgtc agtgtgaata
    17701 cgcagcggcg gatgtctggt atctgttacc gatcaccgcc aagcttatgg tagaaacgga
    17761 ggcctccggc tggctacctg cggcgctgga tgaatgccgc ctgatgcaaa tgcgtcgtca
    17821 ggaagtcgtt gcgccggaag atgcctggcg tgatatcacc aatgcctggc agttacgcac
    17881 acgccaactg gcctgtctgc aactgttagc cgactggcga ctgcgcaagg cgcgagagcg
    17941 tgatctggcg gtgaactttg tcgtgcgtga agagcatttg tggtcggtcg cgcgttatat
    18001 gccgggaagt ttaggcgaac tggacagcct gggtttatcc ggtagcgaaa tccgctttca
    18061 cggtaaaacg ctgctggcgc tggtggaaaa agcgcagaca ttgccggaag atgccttacc
    18121 gcagccgatg cttaacctga tggacatgcc tggttatcgt aaagcgttta aagcgattaa
    18181 gtcgctgatt actgacgtga gcgaaacgca taagatcagc gccgaattgc tggcatcgcg
    18241 tcggcaaatc aaccaactgc tgaactggca ctggaaactg aaaccgcaga acaatttgcc
    18301 ggagctgatt tccggctggc gtggtgagct gatggcggaa gcattacaca atttattgca
    18361 ggaatatccg cagtaaaatc ttccgaagcc ggactgggcg cgctcagcgc cacatccggc
    18421 tttggcaaac tacaaatcca acaccaggcg tttacccttc gcacgcgaac aacagatcaa
    18481 catactttgc tggctggcac gctcttcatc gctaaaatac tgatcccgat ggtcagcttc
    18541 accttccagt attgccgttt cgcaggttcc gcatacccct tcacgacata aacattccac
    18601 tttcgcggct ttattatttt caataacctg caaaatagtc atctcttccg gcaccacaaa
    18661 ctcttttccg gaacgggcaa gcaccagggt aaatgcatcg ccggttttgt cttcgatagc
    18721 aaattgctca aagtgcagtg tatcggcggc gatgtccaga cgcgcagctt cacttcttac
    18781 cgcttcaatt agcgcctcgg ggccacaggt ataaacgtgt gtgccaggtt cgatatccgc
    18841 caataatcgc gctaattcca gtcgtgttcc ggttgatgaa tgatgcaaat ggactttctc
    18901 agcctgcgga tgctggacta gctcagcacg aaatgcgcaa ctttctggat ttcgcgagca
    18961 gtaatgtagc tgccagtcga tgtcgctgtg ttgcaactct gccatgtgcg acaggaaagg
    19021 ggtgatacca ataccgcccg cgataaacag atgctttctg gctgagggaa ttagcgcaaa
    19081 caggttatta ggcgttgaga tcgttaaccg atcgcccact tttacctgct gatgcaaaaa
    19141 gcgggaaccg ccgcgcgagt tttcttccag tcgaacggca atctgataac tagaggtgtt
    19201 atgcggtgaa ctcagtagtg aatacgcatt gctgtactgg ttatcaccat cgctcatctg
    19261 cactaggatg tgacttcctc cggtgaacgc aggcagcggt ttgccatcgg ttgccaccag
    19321 cgtgaagcgt ttcacctgtt cggtaagggg ttcaacctgg ctcacctgta cttcaaacat
    19381 ttgatagtct gacattgctg tactccatca aaaatcagcc cagcggcgga aaataccgcc
    19441 gccgatgtca ttagtcctta aacacctgcg ccagcagatt atggaaatgg gcgataccat
    19501 gttcggaaat gccactaccg ctactgtcgg ccatgatgcg cccctgacca cgatagccac
    19561 gcgatttcag ccctttctgt acgctttcaa ccagacgtaa atcttccgga tggaacacat
    19621 cgcgatacca ctcaatcagc gatttttgct cgtcggttaa ctcttcattg gtgaagtaaa
    19681 tatcgtagtt ttgcagggta gtttcagaat ccaccgggaa ttcataaatg accgtcatca
    19741 tccctttgat cggggtgacg ttcagcatcg tgcacggcca cagccagaaa ccgtggaatg
    19801 ccgcatccgt accctcttca aatttaaacg actgttcgga cggtttggcg aaaccgtatt
    19861 gcagcgtcca gttaccgtgc atggtgtgcc agtaacgatc aacctgtacg gagtcggaga
    19921 aacctagatg cgccggacca cagtgatagc actcgagata gttatcgaca atgttcttcc
    19981 agttggcagg cgtgcgggtg gtaaagcggg ccgctagttt cagattgtgg acttccgggc
    20041 aggcttccag cactttcgcg cccagaccgg tgtattgatt cacttgaagt acgaaaaaaa
    20101 accgggagga cattggatta ttcgggttct gatgggatta gatttggtgg ggcttgcaag
    20161 cctgtagcgc aaattttagt cgttaatcaa tgaaacgcga aagatagtaa aaaattgctt
    20221 ttgtttcatt gaaaatacga aaaacaaaaa cactgcaaat catttcaata acagcttcaa
    20281 aaaacgttca aaaccgataa caaccaggct gtaaccaaat gactcatatc acaaatcagc
    20341 tcatgccgtt taggtatgtt acatgtgtga ttatgtgagg tgaagtatgt tttagctggt
    20401 tcatggttgt tatacggctt tttttacatc ttgtggttct tgtgaaggag ctacaagacc
    20461 ttcctgttca taaataccat actttgacaa aatcttttta cgagtatctg ttggtaatga
    20521 ttcaagcatt ttggctgttt gtaaggtttg ctgcgaaata ccttgcaaat cagcctggat
    20581 ggaatcatcg gaagaaaaaa tctcaggcat aagagcttca aatcctttct ttctgaacaa
    20641 ctcaatcgca gaatgcagtt ccttgtcctc taacaattcg acaattgcga tcagtttagt
    20701 tttcaatgtg ctgcggtcag gttgagaatt tttattctct tgattatttt tatgctcaac
    20761 cattggcatg ctctgacttt ggataaacca ctctattgga cgccctgttg cgtaggctat
    20821 ttgtgctaat cgatctaaag acgggtatgt cttgccagac aggtagtttc tgataacccc
    20881 atcagaaagt ccgcacttag ctgcaaacgc ccggcttgat tctccattca ttgcctcttc
    20941 aaggcgttcc ttgaaacgca ttatttccgg gggcataaaa atgcgctctg catttttgct
    21001 ttctttttca cctgtcatgc ccaatctctc tgttatataa agaattttac agaattcttt
    21061 gatttgcact ggttaaaata aaagtgcgca tagcttttga attgagcatt tttctgcgct
    21121 atcgtttttc ccgatagcgg tagccggtat cagttaccga aaacgttaag agagtaagga
    21181 tcgcagaatg tcagcaaaaa accaagactg gcacgcagag cagataaagg ctgctgttcg
    21241 tatgaagggg aagacactat ctcaattatc cagag
//
LOCUS       DAJTSA010000067        21253 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000067 DAJTSA010000000
VERSION     DAJTSA010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.067"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            18..791
                     /locus_tag="OO369_002663"
                     /pseudo
     CDS             18..791
                     /locus_tag="OO369_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445615.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(851..2149)
                     /gene="eno"
                     /locus_tag="OO369_002664"
     CDS             complement(851..2149)
                     /gene="eno"
                     /locus_tag="OO369_002664"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR5982890.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2237..3874)
                     /gene="pyrG"
                     /locus_tag="OO369_002665"
     CDS             complement(2237..3874)
                     /gene="pyrG"
                     /locus_tag="OO369_002665"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR5982891.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4102..4893)
                     /gene="mazG"
                     /locus_tag="OO369_002666"
     CDS             complement(4102..4893)
                     /gene="mazG"
                     /locus_tag="OO369_002666"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR5982892.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4989..7223)
                     /locus_tag="OO369_002667"
     CDS             complement(4989..7223)
                     /locus_tag="OO369_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR5982893.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7271..8572)
                     /gene="rlmD"
                     /locus_tag="OO369_002668"
     CDS             complement(7271..8572)
                     /gene="rlmD"
                     /locus_tag="OO369_002668"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR5982894.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8629..11385
                     /gene="barA"
                     /locus_tag="OO369_002669"
     CDS             8629..11385
                     /gene="barA"
                     /locus_tag="OO369_002669"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR5982895.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11617..12957)
                     /gene="gudD"
                     /locus_tag="OO369_002670"
     CDS             complement(11617..12957)
                     /gene="gudD"
                     /locus_tag="OO369_002670"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5982896.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12978..14318)
                     /locus_tag="OO369_002671"
     CDS             complement(12978..14318)
                     /locus_tag="OO369_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5982897.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14320..15672)
                     /gene="gudP"
                     /locus_tag="OO369_002672"
     CDS             complement(14320..15672)
                     /gene="gudP"
                     /locus_tag="OO369_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR5982898.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16106..16555)
                     /locus_tag="OO369_002673"
     CDS             complement(16106..16555)
                     /locus_tag="OO369_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR5982899.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16573..17355)
                     /gene="truC"
                     /locus_tag="OO369_002674"
     CDS             complement(16573..17355)
                     /gene="truC"
                     /locus_tag="OO369_002674"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR5982900.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17355..17684)
                     /locus_tag="OO369_002675"
     CDS             complement(17355..17684)
                     /locus_tag="OO369_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR5982901.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18306..18851)
                     /gene="syd"
                     /locus_tag="OO369_002676"
     CDS             complement(18306..18851)
                     /gene="syd"
                     /locus_tag="OO369_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR5982902.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18919..19767
                     /gene="queF"
                     /locus_tag="OO369_002677"
     CDS             18919..19767
                     /gene="queF"
                     /locus_tag="OO369_002677"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR5982903.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19879..21243
                     /gene="ppnN"
                     /locus_tag="OO369_002678"
                     /gene_synonym="ygdH"
     CDS             19879..21243
                     /gene="ppnN"
                     /locus_tag="OO369_002678"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR5982904.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5230 a         5670 c         5066 g         5287 t
ORIGIN      
        1 ttgcagccca accaactatt gtcccacaat tacagcaaca agttacgggt cttacgagta
       61 gcttaaactc acaagaaaag aaagaactga cgcacaagtt agaatctatt ttcaataata
      121 cccaagtgca aattgctgta ttaattgttt ccacaaccaa agatgagaca attgaacaat
      181 atgctacaag agtttttgac aattggcgtt taggagatgc caaacgtaat gatgggatac
      241 tgatcgttgt tgcctagtcg gatcgcactg tccgcatcca ggtaggttat gggctggaag
      301 aaaaggtaac cgatgctctg gctggagata tcatccgtag caacatgata cccgcattta
      361 aacaacaaaa attagctaag ggattagagt tagctataat cgctttgaat aaccaactca
      421 cttcacaaca ccaatatccg gctaatcctt cagaaagtga atcaacgtct tccactgatc
      481 attattactt tgctattttt tgggtatttg cagtgatgtt cttccctttc tggttttttc
      541 atcaaggcag taatttttgt cgcgcatgta aaagtggagt ctgtatttca gcaatccatc
      601 ttttagattt attcctgttc tccgataaaa ctttttccat tgctgtattt tccttctttt
      661 ttactttcac catatttatg gtctttacct gtttatgtgt acttcagaaa agagcatctg
      721 gtagaagtta tcattcagac aacagcggtt ccgctggagg ctcaagtggc ggcgcatccg
      781 gccgctggta actccaccat aaaaaatgcc agcccgaagg ctggcattat caaatcagat
      841 aaagtcagac ttatgcctgg cctttgatct ctttacgacc gttgtacggt gctttttcgc
      901 ccagagcttc ttcgatacga atcagctggt tgtatttagc aacacggtca gaacggctca
      961 tagaaccagt tttgatctgg cctgcagcag taccaacagc cagatcagcg atggtagcat
     1021 cttcagtttc accagaacgg tgagagataa ctgcagtgta gccagcatct ttcgccatct
     1081 tgattgcagc cagagtttcg gtcagagaac cgatctggtt gaatttgatc aggatggagt
     1141 tagcgatacc tttttcgata ccttctttca ggatcttggt gttggttacg aacaggtcgt
     1201 caccaaccag ctggattttg tcgcccagaa ctttggtctg gtatgcgaaa ccgtcccagt
     1261 cagattcgtc cagaccgtct tcgatagaaa cgatcgggta ctgtttggtc agttcttcca
     1321 ggaagtgagt gaattcttca gaggtgaacg ctttgttgcc ttcgccagcc agaacgtatt
     1381 taccatcttt gtagaattca gaagctgcgc agtccatcgc cagagtgatg tctttgccca
     1441 gttcataacc agcagcttta acagcttcag cgataacagc cagagcttca gcgttggaac
     1501 ccaggttcgg cgcatagcca ccttcgtcac caacagcagt gttcatgcct ttcgctttca
     1561 gaacttttgc caggtgatgg aaaacttcag aacccatgcg gatggcttct ttcacagttt
     1621 tcgcgccaac cggctgaatc atgaattcct ggatatcaac gttgttgtca gcgtgctcac
     1681 caccgttgat gatgttcatc atcggaaccg gcatggagta tttgcccgga gtgccgttca
     1741 gttcagcgat gtgctcgtac agcggcatac ctttagcagc tgcagcagct ttggcgttag
     1801 ccagagatac agccaggatt gcgttcgcgc cgaatttgga tttgttttcg gtgccgtcca
     1861 ggtcgatcat gatcttgtca atgccagcct gatctttagc atctttgcca atcagcgcct
     1921 gagcgatcgg gccgtttacc gcagcaacag ctttggttac gcctttaccc aggaaacggg
     1981 atttgtcgcc atcgcgcagt tccagagctt cacgggaacc agtagaagca cctgacggag
     2041 cagctgccat accgacgaaa ccaccctcca gatgtacttc ggcttcaaca gtcgggttac
     2101 cacgggagtc gatgatttca cgaccgatga tttttacgat tttggacatt aggttttcct
     2161 caagtcacta gttaaactga aactccagac aaacaacgcg tacccaaggt acgcgttgcc
     2221 gttctaactt ttttacttac ttcgcctgac gtttctggaa ctcgctggcg gctttcacaa
     2281 agcctgcaaa cagcgggtga ccatcacgtg gagtagaagt aaactccgga tggaactggc
     2341 aagccacgaa ccacgggtga ttcggaactt cgatgatctc gaccaactga tcatccccgg
     2401 aacggcccgc aacgcgcaga cctgcatctt caatctgttt caacagcatg ttgttgactt
     2461 cgtaacggtg acgatgacgc tcaacaattg tcggcgcatt gtatagctgg cgaaccaggc
     2521 tatcgtcaac caactggcac tgctgtgcgc cgagacgcat ggtaccgccg agatcgctct
     2581 tctcgctacg aacttcaacg ttgccgtttt catcgcgcca ctcggtaatc agcgccacaa
     2641 ccgggtactt acagtctggc acaaattctg tagagttggc gttctccatg ttggcaacat
     2701 ggcgagcgta atcaattaac gccacctgca tacccaggca aatgcccaga taaggaatat
     2761 tgttctcacg cgcaaaacgc gcggtcgtaa tcatgccttc tacgccacga tagccgaaac
     2821 cgccaggtac gaggattgcg tccagacctt taaggatttc aacgccgcgc gtttcaacat
     2881 cttgtgaatc gatcagtttg atgttgacgc tgacacgatt cttcagccca ccgtgtttca
     2941 gtgcttcgat cactgattta taagcatccg gcagttcaat gtacttgccg accataccga
     3001 tggtgacttc acttaccgga ttcgcttctt cgaagataac ctgttcccat tcggacagat
     3061 ttgcttccgg gcagtttaag ctgaatcgtt tacaaatata atcgtccagc ccctgagatt
     3121 tcaacaggcc cggaatttta tagatggaat cgacgtcttt cagagaaata accgcttttt
     3181 ccggaacatt acagaacaat gcaatcttcg cacgttcgtt cgccggtaca gcgcgatctg
     3241 aacgacaaat caggatgtca ggctggatac cgatggagag cagctctttt acagagtgct
     3301 gagtcggttt ggttttgact tcaccagacg ctgccatgta cggcaccagc gtcaggtgca
     3361 taaacagagt gtgctcacgg ccaatttcaa cagccatctg gcgaatagct tcgaggaacg
     3421 gcaaggattc gatatcacct actgtaccgc cgatttctac cagtactacg tcatgacctt
     3481 cgccaccttc cagcacgcgc tctttgattg cgttagtgat gtgcggaata acctgcacgg
     3541 ttgcgccgag gtagtcaccg cggcgttctt tacgcagaac gtcagagtag atacgacccg
     3601 tggtgaagtt gttgcggcgg ctcattttgg tacgaatgaa acgctcgtag tgccccaggt
     3661 ccaagtcggt ttcagcgccg tcttcagtaa cgaacacttc cccgtgttgg attgggctca
     3721 tagtacctgg atcgacgttg atgtaaggat ccagtttcat gatggtcaca ttgaggccac
     3781 gggcttcaag aatggctgcg agggaggctg cggcaatgcc tttacccaga gaggatacga
     3841 ccccgccggt cacaaaaata tagttcgttg tcatgctgaa cctgagaagt taggttgaaa
     3901 agacgatgga ataaccaaga cgggaaagca gtatacccga acatgacctg tgccacaaac
     3961 tttcattatc cctcctcttc gccagcgcac tattgaaatc aggagtgaga aaatagcccc
     4021 tttggggtaa atgtttttga cgcaaatcaa gcgcttgtca tttaaaaaat gacacaaatg
     4081 gcgcttgacc gcgtaattcc cttagagatc aatttcctgc cgttttacct gttgccagac
     4141 ttcttccatt gtttcgaggt caacacctgt catttccagt ccacgcgcgg caacaatacg
     4201 ctccacttcg cgaaaacgac gctcgaattt ttcgttcact ttttgcaatg cgatttctgc
     4261 tttcgtccct aaatggcgag ccagattaac cgtggcaaac agcaggtccc ccatttcctc
     4321 ctccagttta gcctggtcga caacagcctg ccgcgcctcg tacatcacct cgtcgatctc
     4381 ttcgtagact ttatcaacca ccgggccaag cgtcgtccag tcgaagccaa cgttggcgca
     4441 acgtttctgg attttttgcg cccgcattaa agccggtaaa ctacgaggaa tatcgtccag
     4501 cgccgaatgc tgcgctttct gcgcgcgctc ttcggttttg atttgctccc aacgggcaag
     4561 cacttcacta ctgttttcgg cagaactatc agcaaaaaca tgcggatggc gacgctctaa
     4621 tttatcgcta atagcagcgc aaatatcatt aaagtcaaag cgcccttctt cctgagccat
     4681 ttgcgcgtaa aacaccacct ggaatagcag atcgcccagt tcaccgcgaa gatcgtcaaa
     4741 atcttcacgg gcgatggcgt ccagcacttc gtaggtttct tcaagggtgt aaggcgcaat
     4801 ggtggcaaat gtctgctctt tatcccacgg gcagccgttt tccggatcgc gcaggcgctg
     4861 cataatagtg agcaaacggt cgatttgatt cattgaattg tcctgaaaat tgcgggtctg
     4921 tcaggtgtaa taccttcgag taaaagatat cgctggatac ggctgacgcc atatccagct
     4981 atgtaagact aactcccgtg caaccgacgc gcgtcgataa catccggcac ctggttgagt
     5041 ttacccagca cgcgccccag cacttgcagg ttgtaaatct caatggtcat gtcgatggtc
     5101 gccagttgct gtttggtgtc gctacggctg gcaacgccaa gcacgttcac cttctcgttg
     5161 gcgagaatgg tcgtgatatc acgtaacaac ccactacgat cattagccac cacgcggacc
     5221 accagcgaat atccggcgga gtagctctca ccccataccg cgtcaacaat gcgttctggc
     5281 gcatgggagc gcagttccgc cagttgttcg caatcggcgc ggtgtactga aataccgcgt
     5341 ccctgggtaa tgaagccgac aatctcatct ccaggaatcg gctggcagca gcgcgcgatg
     5401 tggtgcatca ggttaccaac accttcaacc accacacgac cgttatcttt actgcggttt
     5461 tgcggtgtgt agcttttttg ctgaagctgt ttcagcgcgg cggcgtcctg ctcttcggca
     5521 ctcggcttat taaattgcga ttgcaggaag ttcaccatct gattgagacg gatatccccg
     5581 ccaccaatcg ccgccagcaa ctcgtcgaca tcattgaagt tgtaacgcgg cagcagatgt
     5641 ttttctgctt ctttcaggct gatccccaga tgttccagct cgtcgtcaag gatttgccgc
     5701 ccagccagaa tgtttttgtc acggtcctgt ttacggaacc aggcgtgaat tttcgaacgc
     5761 ccacggctgg ttgtgacgta accgaggttt gggtttaacc agtcacggct ggggttcggc
     5821 tgtttctggg tgataatttc aatctggtcg cccatttgca gctggtaggt gaacggcaca
     5881 atgcgcccgc caatttttgc cccgatgcag cggtgtccga catcactgtg gatgtggtaa
     5941 gcgaagtcca gcggcgttga tcccgcaggc aaatcaacga catcaccttt cggcgtaaag
     6001 acatacaccc ggtcgtcaaa cacctgacta cgtacttcgt cgagcatttc gccggaatca
     6061 gccatctctt cctgccacgc aatcagttta cgcagccagg caatccggtc ttcatgtccc
     6121 gaacgtgcgc cgccagctgc cgcgccctct ttatatttcc agtgcgcagc aacacccaac
     6181 tccgcatctt catgcatctg tttggtgcgg atttggatct caacggtttt tccacccggc
     6241 cccagaacca cggtatgaat agactgataa ccgtttggtt tcgggttagc gacgtaatcg
     6301 tcaaactcat ccggcaggtg gcgatagtga gtgtgcacta tccccagtgc ggcataacaa
     6361 tcctgtaaac gctcggcgac aatacgtacc gcacgcacat caaacagctc atcgaaggcg
     6421 aggttctttt tctgcatttt gcgccagatg ctgtagatgt gtttcggacg accatacact
     6481 tcagctttaa cgccttcagc tttcatctca gcgcgcagat ggccgacaaa ctcttcgata
     6541 tagtgttcgc ggtcgagacg gcgttcatgc agcagttttg cgatgcgttt gtattcggtc
     6601 gggtggagat aacggaagca gtaatcttcc agctcccatt tcagttgccc aatcccaaga
     6661 cggttcgcca acggcgcgta gatattggtg cactcttttg ccgccagtac acgttcatct
     6721 tccggcgcat cttttacttc gcgcagatga gcaatacgct ccgccagttt gatgactacg
     6781 cagcgaaaat catcgaccat cgccaataac atccggcgaa cgttatcgac ctgttcggag
     6841 gaaacagaat cagtgtgcgt cgctttcagc tggcggatcg ccgccatatc acgcacgccg
     6901 tgaataaggt taacaaccga cttaccgacg ctctcacgca gcacatcttc gctgactacg
     6961 ttggcatcag ccagagggaa cagcagcgcc gcccgcagcg tgtcaatgtc catacttaat
     7021 gtcgagagga tctccaccat ctcaacacca cgccacaata acagactggc atccggatgc
     7081 ccctgcgtct gttgcagaca atacgcccag gtttcggcta agcactcaca cgacttctgg
     7141 ctggtaatac ccagacttgc gatccatttt tccggatcaa attcaccagc cttattgata
     7201 tgtgcacttc ttaccgcaac catcgtcctc tcctttaggg accagacctg ccgaaatcgg
     7261 caaatcgtaa ctatttaacg cgcgagaaaa gtaccatcga ttccagatgt cccgtgtgtg
     7321 ggaacatatc cagcatcgcc agtcgcgcaa tggtatatcc tgcttttaat aacgcttcgc
     7381 tatcccgagc cagcgttgca gggttacagg atacataaac tatacgaata ggttccagtt
     7441 ttataatttg ctgcataaca cctgcggcac ctgctcgcgc cgggtccagc aacactttat
     7501 cgaagccgtt tttcgcccac ggctgctttg tgacatcttc ttcaagattt tcgtgataaa
     7561 acgtcacatt ctgtaagcca ttaagacgcg cattctgctg gcctttttcc accagcgcag
     7621 gaacaccttc tacaccgacc acactggcag cttgtgtcgc caatggcagt gtaaagttgc
     7681 ccataccgca gaacagatcc agtacgcgat cttcaggttg cacatccagc cattccaacg
     7741 cacgcgctac cattttttgg ttcacacccg cattgacctg aataaaatcg cgcgggctaa
     7801 aagttaagcg caacccgttt gagtcatacc agggcatctc accagagacg gtttcgagta
     7861 tctcactatc gggggcgaga tacagatcca ggccttcaga atgcgaaaag cgttccagtt
     7921 tttcgcgatc tgccgaactt agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg
     7981 ttgcctgtac cagttcaaca tgaccaagat ggcgcatagc ttgtaagctg cccagacatg
     8041 ccctgacttt gggcagcaat gcttcaagtt ggggcgctaa aatggggcat tgtttgacgt
     8101 cgacaatgtc actggagccc gctttgcgaa accccatctg aagttgctgt gttttcggta
     8161 agtagttcag acttaaacgc gcgcggcggc gatagcccca gggaacatcg gcgatcactt
     8221 cagagacatc gtgtttcatt aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat
     8281 ccacgctggc gtgttgttgc tgacagccgc cgcatacgcc aaaatgagga cagcgtggcg
     8341 tttcgcgttc cgggctatcg cttaaccggc gtacgacttt agcgcgggca tactgttttt
     8401 tatcttcagt aacagcaact tccgcgtttt cctgcggcaa taatccgggg ataaataacg
     8461 ttttgccgtt atgtcgcgcc acgccctgac caaaagagtc gaggtcgttg actgaaacgg
     8521 ttatgatctg acgcgtcgtc gtgcgtcgtt ttgcagagta gaattgcgcc attggcgaga
     8581 ctttctcaat ttaacagtgt gaccttaatt gtcccataac ggaactccat gaccaactac
     8641 agcctgcgcg cacgcatgat gattctgatc ctggcaccga ccgtccttat tggtttattg
     8701 ctgagtatct ttttcgtcgt gcatcgctat aacgacttgc agcgtcaact ggaagatgcc
     8761 ggtgctagca ttattgagcc gcttgcagtt tctactgaat atggcatgag cctgcaaaat
     8821 cgcgaatcta tcggtcagtt aataagcgta ctgcatcgtc gccattccga tattgttcgc
     8881 gcgatttcgg tttatgatga aaataaccga ctctttgtca cctccaattt tcatcttgat
     8941 ccctcatcaa tgcagctcgg cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt
     9001 gacggcgata ttatgatcct ccgcacgccg attatttctg agagttactc ccccgacgaa
     9061 tcgcccagta gcgatgccaa aaatagtcaa aatatgttgg gatatattgc gcttgagctg
     9121 gatcttaaat cggttcgctt gcagcaatat aaagagatct ttatttcctg cgtgatgatg
     9181 ctgttttgta tcggtattgc gcttattttt ggctggcgct taatgcgcga tgtaaccggt
     9241 ccgattcgca acatggtgaa taccgtcgac cgcatccgtc gcgggcaact cgacagccgg
     9301 gtggaaggat ttatgctcgg cgagctggat atgctgaaaa acggtatcaa ctcgatggca
     9361 atgtcgctgg ctgcttatca cgaagagatg cagcacaata tcgaccaggc gacatccgat
     9421 ctgcgtgaaa cgctggagca gatggaaatt cagaacgttg agttggatct ggcgaaaaag
     9481 cgcgcccagg aagcggcgcg tattaaatcc gagtttctgg caaatatgtc acacgagctg
     9541 cgtacaccac tgaatggtgt tattggcttt acccgcctga cgctgaaaac agaattaaca
     9601 ccaacgcagc gcgatcacct gaatacgatt gaacgttcgg caaataattt gctggcaatt
     9661 attaatgatg ttctcgactt ctcgaaactg gaagcaggta agctgattct ggaaagtatt
     9721 ccattcccac tacgcagcac gctggatgaa gtcgttactc tgctggcaca ttcttctcac
     9781 gataaagggt tggaactgac gctcaatatt aaaagcgacg tgcctgataa cgtgatcggc
     9841 gacccactgc gattacagca aatcatcact aacctggtgg ggaatgcaat taaattcacc
     9901 gagaatggca acattgatat tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag
     9961 attgaagtgc agattcggga taccggcatt ggtattcctg aacgcgatca atcgcgctta
    10021 ttccaggcct tccgacaggc tgatgccagt atttcccgcc gtcatggtgg caccgggctg
    10081 gggctggtga ttacacaaaa actggttaat gaaatgggcg gcgatatttc gttccatagc
    10141 cagccgaatc gcggttcaac tttctggttc cacattaatc tcgatctgaa cccgaacatt
    10201 attatcgaag ggccatccat ccagtgcctc gcaggtaagc gcctggccta tgtcgaacca
    10261 aactccgcag cagcgcaatg cacgctggat attttaagtg aaacgccgct ggaagtggtt
    10321 tatagcccaa cgttctccgc gctgcctccc gcgcattacg acatgatgtt gttaggcatc
    10381 gcggtgacct tccgcgagcc gctaacaatg caacatgagc gattagcgaa agcggtatcg
    10441 atgaccgatt tcctgatgct ggcacttcct tgccatgcac aagttaatgc tgaaaaactc
    10501 aagcaagatg gtatcggcgc gtgtctgctg aaaccattaa cacctacgcg cctgttgcct
    10561 gccctgacgg aattttgtca tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagt
    10621 aagctggcaa tgacagtcat ggcggttgat gacaaccccg ctaacctgaa acttatcggc
    10681 gcattgctgg aagatatggt gcaacatgtg gaactttgcg atagcgggca tcaggcagtt
    10741 gaacgggcga aacagatgcc gttcgatttg atcttaatgg atattcaaat gccggacatg
    10801 gatggcattc gggcctgcga actcatccac caactcccgc atcagcgaca aacgccggtt
    10861 atcgcggtaa cggcgcatgc aatggccggg caaaaagaga agctgcttgg cgcagggatg
    10921 agcgattatc tggcgaaacc gattgaagaa gagcgattgc ataatttgtt gttgcgctac
    10981 aagcctggca gcggtatttc gtctcgcgtc gtgacgcccg aagtcaacga aattgtggtg
    11041 aacccgaatg cgaccctcga ctggcaactg gcactacgcc aggcagcagg aaaaaccgat
    11101 ttagcgcgcg atatgctgca aatgttactc gatttcctgc ctgaagttcg caacaaagtt
    11161 gaggaacagc tggctggaga aaacccggaa ggcctggttg atttgattca taaactgcat
    11221 ggcagttgcg gctatagcgg cgtaccacgt atgaagaatc tctgccagct gatcgaacaa
    11281 cagctacgta gcggtactaa agaagaagat ttggagccgg agctgctgga actgttggac
    11341 gagatggata atgtcgcgcg cgaagccagc aaaattctcg ggtaacggac gtcttgtcga
    11401 gtagcaatcg gctttttatg acgctggttt cagattggtg acaaagtgcc tgtgtttatg
    11461 ccggatgcgg cgttaacgcc ttatccggcc tacaaaacca attaaattca atgaattaaa
    11521 aaatgatgta gacctgatac atagcacaac aggctatttt gcgtttagca tcagtctcaa
    11581 accggctcca gatagagccg gttttggttt tctgtcttaa cgcaccatgc acgggcgctt
    11641 gttatcgaac gtccagccag gaatcagata ctgcattccc atcgcatcgt cacgcgcgcc
    11701 aagcccgtgt ttctgataca gctcatgggc tttcatcact tgatccatat cgatttctac
    11761 acccagcccc ggtttttctg gcacctgtac cagcccgcct ttgatctcaa acggttcttt
    11821 ggtcaggcgc tgattacctt cctgccagat ccagtgcgta tcaatagcag taattttacc
    11881 cggtgcagcg gcggcaacat gggtaaacat cgccagggaa atatcgaagt ggttgtttga
    11941 gtgtgaaccc caggtcaggc caaattcatg gcacatttgc gccacacgta ccgaaccttg
    12001 catagtccag aaatgcggat ccgccagcgg gatatcaacg gattgtaacg agagcgtatg
    12061 gcccatctgc cgccagtcgg tggcgatcat attggttgca gtcggcaggc cagtcgcgcg
    12121 acggaactct gccatcactt cacgcccgga gaaaccttgc tccgcaccac acggatcttc
    12181 tgcataagcc agcgaacctt tcaggtattt accaatttta attgcttcgt tcagcgacca
    12241 ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaag cgttgcgcca gtgccacaat
    12301 agactcggct tcttcttccc cggccagtac accgcctttc agtttgaaat cgttgaagcc
    12361 atatttttca tacgccgctt ccgccaggcg caccaccgca tccggcgtca tcgcttcttc
    12421 atgacgcagg cgataccagt cgcatgagtc atccggctgg ctttgatacg gcagaggtgt
    12481 ggctttgcga ttaccgacga agaacagata accgagcatt tcgacttcgc tacgctgttg
    12541 accatcgccc agcagcgatg ccacgtttac ccccagatgc tgccccagca gatccagcat
    12601 tgccgcttct atcccggtaa ctacatgaat agtggtacgc aaatcaaatg tctgcaaacc
    12661 gcgcccacta gcatcacgat cggcaaaagt attacgcacc agcgtcagaa cgtttttgta
    12721 ttcacccagc gttttaccta ccaccagcgg aatcgcatct tccagcgttt tacggatttt
    12781 ctcgccgccg ggaatttccc ctacgccagt gtgaccggaa ttatctttga taatcacaat
    12841 attacgcgtg aagaacggtg cgtgtgcacc actcagattc atcagcatac tgtcatgacc
    12901 cgccaccggg atgacctgca tttcagtaac aacaggcgtc gtaaattgag aactcatatt
    12961 tatgtccttt ttcagaatca atgacggccg aaaacgggac gtttacggtc aaaggtccag
    13021 ccggggatca ggtactgcat cggacctgcg tcgttacgcg caccgcaagg cagacgttta
    13081 taggcctcat gtgccttttg tacctgttcc cagtccagtt ccacgcccag accgggcgca
    13141 tcaggaacgg caatttttcc gtttttaatc tccagcggat ttttggtcag gcgacaatcg
    13201 ccctcctgcc aaatccagtg ggtatcgata gcggtaggat tacccggtgc cgccgcgccc
    13261 acatgggtaa atatcgccag agagatatcg aaatggttgt tggaatggca gccccaggtc
    13321 agcccccagt cgtcgcaaag ctgcgccacg cggactgcac cggaaagcgt ccagaagtgc
    13381 ggatcggcaa gtggaatatc taccgcattg agcatcaccg catgacccat ttcgcgccag
    13441 ttggtggcga tcatgttagt cgcgacgggc aagccggtcg cccgtcgaaa ctccgccatc
    13501 acttcacgtc cggagaagcc ctgttctgcg ccgcatggat cttcggcata ggtaagaaca
    13561 tcattcagcc ctttgcataa agaaatggct tcatcaagca gccatgcacc gttgggatca
    13621 acggtaatcc gcgcatccgg gaagcttttc ttcaatgcac gaacagtgtc gatttcttgc
    13681 tcgccaggta acaccccgcc cttaagtttg aaatctttaa agccatagcg atcctgtgag
    13741 gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt tctgatggcg caactgatac
    13801 cactcatggt tgcccggcgt attttccaga taaggaagat cggttttggt ccgatcacct
    13861 atataaaaca gataaccgag gacggtaata gtctcgcgtt gcttgcctgg ccccaacagt
    13921 tcgcagaccg gaacattcag cgccttacct agcaggtcaa gcaaggcggc ttccagcgcc
    13981 gccacggcat taacgcgcaa ttcaaaagtc caggcacctt tgccaaaggt atcaaaatcg
    14041 gctgcctgat tacctttatg tacctgctga accactttat tcaggcgcgc aacttcctgg
    14101 cccagaacca tcggaatagc atcgaccagt gtttgataaa tcacctctcc gcccggcgct
    14161 tcaccaatgc cggtatgccc ggcgttatcg gtgagtacca cgatattgcg ggtgaagtat
    14221 gcgttatgcg caccaccaat attgagcaac atgctgtcat gtccggcgac cggaatgact
    14281 ttcatatcag taataacagg actggattgt gtcgccatct tattgccccg cgacaggttt
    14341 cagctcgata cgcttgatat cgcccaccag caccaggtag ctcagtaccg cgattagggc
    14401 atgaacacca acataaatca gcgccccatt aaacgagcca gtcgtgccaa cgatataacc
    14461 aattgcgatt ggcgtgacga tgccagaaat gttaccgaac atgttgaaca ggccaccgga
    14521 aagaccgctg atctcttttg gcgcggtatc tgccattact gcccaaccca gcgccccgat
    14581 gcccttaccg aagaaggcca gcgccataaa gccgatgatc atccactcaa cgttgacgta
    14641 gttgcagaac accatcacca tcgacagcaa catgcccatt acgatcggtg ttttacgcgc
    14701 aatgttcagc gatcccgtgc ggcgcatcag ccagtcggaa ataattccac ccagcacacc
    14761 gccgataaaa ccgcaaaccg ccggaacgga agccacaaag cccgctttca gaatcgacat
    14821 cccacgcgcc tgcaccagat aaaccggaaa ccaggtaatg aaaaagtaag tcagggcgtt
    14881 gatacagtac tgaccgatat aaacgccgat catcatccgt gacccgagca gctgtttgat
    14941 ctgcccccac ttcacgctga acggcacttt aactttggtg ctttgctgat ccatattgat
    15001 cagcgcgcca cccgcggcga tatactccag ctctttctga tttacccccg gatgttgatt
    15061 tggctcgtgg atgactttca accagataaa gctgatgaca atgcccagac cgcccataaa
    15121 gaagaagacg tgtgaccagc ccacttcatg cgtcagccag cccataatcg gcgcgaagat
    15181 caccgttgcg aagtattgag cggagttaaa aatcgacacc gccgttcccc tttcctgcac
    15241 cggaaaccag gccgcaacaa tgcgactgtt gccggggaaa gatggcgctt cagcaagccc
    15301 gaccaggaag cgcagagtaa acagggcaac gataatgccg aatccactaa agatatcgac
    15361 gaagccttgc agcaaggtaa acatcgacca gataaagatc gaccagaagt agacgcgttt
    15421 tgaaccaaaa cgatccagca accaaccacc agggatctgc ccgataacat aagcccatga
    15481 gaaggcagag aacacatagc ccattcccac gggatcaagg ccgatatctt tggccatttc
    15541 cgaaccggca atagaaagcg tagcgcggtc gccgtagttg aaggatgtga cgataaacaa
    15601 catcaccact atccagtaac gagcattcgt gcgtttttcc acactgctcg cagcctgact
    15661 taaagaactc attgttgcac tcctgaaaat tcgcgttagc cacgctcact ctggactgcg
    15721 acatcgccag gaaatcagag gtgacgtagg gtgttttttg ccgtttttat aggtcgttcg
    15781 ccgaatacgg cgcgtgttta tatctggcaa tagcagtata aaaagcgcgc catagcggct
    15841 caccgtgcaa caacacaaca ttaatgcgtt caatgaggcc cgattttggc attagccctg
    15901 gacggtggaa tccacttcac ggaaatgaaa acaagaacaa gaaaggaagg gtaaaaacga
    15961 agaaataaag aagagtatga aatggatcgc ttgactctag gcaaacgcca gtaaaaatcc
    16021 gcgctatgaa gcagttttta ctggcatttg cctgaaaaga ttcgattcag caccgctaaa
    16081 acgacattta ccgctcgctg acatatcagg acaacagcgt gccccactgt tcgacccacg
    16141 gatttgattc cgtttccggt tccgggttct cactggcgtc aatcaacagc atttcgccaa
    16201 cccgctgagc gctctgttcc tgcaacaagg catcgaactg tttgccgcca ttgcaaaaat
    16261 ttacataact gctgtcgccg agcgcaatca cgccataacg cagattcggc tggaagccca
    16321 gactatcttt gattccctga aagagcggca caatgctatc aggaaggtcg ccctgcccgg
    16381 tcgtggacgt aaccaccaga acatacttat cctgataggg cagccagtcg cttaattcag
    16441 gatcttcaaa taccgttgct ttgtggccct gtgcggtcag aattgcttct gcttcttccg
    16501 ccactaacag cgaatttccg tacatggtgc cgacaaaaat accaatttcc gccataccgt
    16561 tttctccctg gattaggaac ttatctctcc atcctgaccc gatggcgcac tgaactcaac
    16621 cctttcattt tcaggaagca gaccgcgcca gccaaattgt gataacgcct gcatccaggt
    16681 gtcgtccagg cccgcgtgaa tagtcagcgg ctcgccagta aaaggatgcg tcaatgacaa
    16741 ctgactggca tgtagcatta accgctggag gccaaaatgc tcagcaccgc tgcgattctg
    16801 gcgtaaatcg ccatgtttgc tatcgccaat aatcggatga cgcaaatggg caagatgtcg
    16861 tcgaagctga tgtttgcgtc cggttttcgg ctccagttcc accaggccgt agcgcgtggt
    16921 cgggtaacgt ccggtcgcta ccggcatttc tacggtcgcc agaccgcgat aatgcgtcac
    16981 tgctggctgc gggcctttat cttcgcgggc aaatttatca gcgattttgt ccagttcttc
    17041 caccagtgga taatccaaca ccgcttcttc catcaaccag ccgcgcacaa tcgcatggta
    17101 acgtttctgg atttggtgct gttcaaactg ttgtgccagc agccgtccgg cctcgctgga
    17161 taatcccatc aacaacacac cagaagtggg tcggtccaga cgatgagcag taaaaacatg
    17221 ctgtcctatc tggtcacgca cggtttgcat gaccactact ttctcgtcgc gatccagcca
    17281 gctacggtga accagccagc cggagggttt atttaccgca accagccatt catcctgata
    17341 gagtatttcc agcattagct cgcatcatcc gcaaaaagag catccagttt ttccaactca
    17401 gccagaataa gcgcgcgttg cggatggtcc gtcgccagcg ccatttcata atagggtgca
    17461 acagcaaaag cgcccggtaa cggctggttg ttatttaaca aatcgtgcat tcgcgggatc
    17521 agcccccact gcaaccactc cagtggttcc atggtgtcca taaagaacgg ttgggtacta
    17581 ttaaattgat gcggctgggg ttcatcgttt cgccagtgct gatgttcacg cagtaacgct
    17641 tcaagcgcct gcaactggag acgaacgcgg tcatgagtgg tcataaagca acctcaataa
    17701 gaaaaactgc cgcgaaggat agcaggaata aaaaaaggga gcactgtatt cacagtgctc
    17761 ccggttcgtt tcgcagcatt ccagctactt ttgttgctcc ctgctcatcc ttgacaactt
    17821 ttcctctggc cttgcggcca atcgttcatc ctgaactatt gcttcctgct cacaccaccc
    17881 cgatgtgata cttcatcctg aagtgtccct ggccttcctg acccaccgaa tcatcctgac
    17941 cggttctcat tctccatcct ggaggtgtcc tttaacgcgt cctgcgtcat cctcttcgct
    18001 tcatccagaa gcctttccct gaaacaccat cctggtgtgt cctgcagaag tgtcatcatc
    18061 ctgatgttca cttcgttgtc tgactccctg tcgacgaaga tagaatcgtc tttttcgcga
    18121 agtcttacaa ggcgcttaca gacaatgcct taaggaaaat ttcatatgaa agttaagaat
    18181 taacaatcaa cattttgatt ttaaatgaaa ataacgattc ttatcttttt aagtattcgc
    18241 ttatttcctg gcgatctcct acagagtctg taagagatct ctcacaacca gatggggtat
    18301 ttggattaca gaagaggctt aagttgatta aggaattccg caagatttga ggctaaattc
    18361 gtccgtttac gtgttcccag cgtctctttg cacacttcac cggagagatt acacaccgag
    18421 atgacctcta actcttcttc cagcgtcgcg ataaatagcg tgggcggcag ttttagcctt
    18481 ttctgagtca ccagatgacc aatcagattt tcctgcaccc gacggaagtc gtcttcactc
    18541 caggtttgca gcaatgtcag tttgatatcg ccaaactgcg cgtgcatatc cccggcaaac
    18601 tgagtcgtat aaaaggtatg aattgtgggt tgtatcacaa tatcaaaagc gcgttcaacc
    18661 gcgtttacat tttgttcccc ggtaaacggc tggggttgcc agtatacggc atcttcggta
    18721 gttgaaataa tgcacggtga aggaacaccg tacaattcct cacttaacgg ccagctttta
    18781 tgctcttcat gccacgcatc gcagtagcgt gcagtaaaat ctttcagggc ttgtgcggtc
    18841 aaatcgtcca ctaatttctc tcttcactta aaccagatac acttgtcgtt tagtttatct
    18901 ggtttatgac ggtgaaacat gtcttcttat gcaaaccatc aggcacttgc gggcctgact
    18961 cttggaaaat caaccgatta ccgggatacc tatgacgcca gcctactgca aggcgttcca
    19021 cgcagcctga atcgcgaccc gctgggtctg aaagcggata acctgccttt tcacggtacg
    19081 gatatctgga cgctgtatga actttcctgg ctgaatgcga aaggtttgcc gcaggtcgct
    19141 gtcggtcatg ttgaacttga ttacaccagc gtaaatctga ttgagtcgaa gagttttaag
    19201 ctctatctca acagttttaa ccagacgcgt tttaataact gggatgaggt gcgccagacg
    19261 ctggagcgcg acttaagcac ttgcgctcag ggtaagatta gcgtggcgtt atatcgtctt
    19321 gatgaactgg aaggccagcc gataggtcat ttcaatggca cttgcattga tgaccaggat
    19381 atcactatcg ataactatga attcactact gactatctgg agaatgccac cagcggtgaa
    19441 aaagtagtgg aagagacgct cgtcagccac ctgctgaaat caaactgcct gatcacccat
    19501 caaccagatt ggggttcgat ccaaattcag tatcgtggac gccaaattga cagagaaaaa
    19561 ctgctgcgtt acctggtctc attccgtcat cacaacgagt tccacgaaca gtgcgtggaa
    19621 cgcatcttta atgacctgtt acgcttctgc cagccagaga aattgagcgt ttacgcacgt
    19681 tatacccgtc gtggcggtct ggacattaac ccgtggcgca gtaatagcga ttttgtccca
    19741 tcgaccacaa gactggttcg gcaataaatt ttttctcaat tttgcgtgct ggattcacgc
    19801 agaaggttgt gaaaggtcat caggcagggc tattgtaatc aaagggaatg acgatattcg
    19861 tcccataagg agtttttctt gattacacat attagcccgc ttggctccat ggatatgttg
    19921 tcgcagctgg aagtggatat gcttaaacgc accgccagca gcgacctcta tcaactgttt
    19981 cgcaactgtt cacttgccgt actgaactcc ggtagtttga ccgataacag caaagaattg
    20041 ctgtctcgtt ttgaaaattt cgatattaac gtcctgcgcc gtgaacgcgg cgtaaagctg
    20101 gaactgatta atcccccgga agaggctttt gtcgatgggc gaattattcg cgctttgcag
    20161 gccaacttgt tcgcggttct gcgagacatt ctcttcgttt acgggcaaat ccataatacc
    20221 gttcgttttc ccaacctgaa tctcgacaac tccgtccaca tcactaacct ggtcttttcc
    20281 atcttgcgta acgctcgcgc gctgcatgtg ggtgaagcgc caaatatggt ggtctgctgg
    20341 ggcggtcact caattaacga aaacgagtat ttgtatgccc gtcgcgtcgg gaaccagctg
    20401 ggcctgcgtg agctgaatat ctgcaccggc tgtggtccag gagcgatgga agcgccgatg
    20461 aaaggtgctg cggtcggaca cgcgcagcag cgttacaaag acagtcgttt tattggtatg
    20521 acagagccgt cgattatcgc cgctgaaccg cctaacccac tggtcaacga attgatcatc
    20581 atgccggata tcgaaaaacg tctggaagcg tttgtccgta tcgctcacgg catcattatc
    20641 ttccctggcg gtgtgggtac ggcagaagag ttgctgtatt tgctgggaat tttaatgaac
    20701 ccggccaaca aagatcaggt tttaccattg atcctcaccg gcccgaaaga gagcgccgac
    20761 tacttccgcg tactggacga gtttgtcgta catacgctgg gcgaaaacgc gcgccgccat
    20821 taccgcatca tcattgatga cgccgctgaa gtcgctcgtc agatgaaaaa atcgatgccg
    20881 ctggtgaaag aaaatcgccg tgatacaggc gatgcctaca gctttaactg gtcaatgcgc
    20941 attgcgccag atttgcaaat gccgtttgag ccgtctcacg agaatatggc taatctgaag
    21001 ctttacccgg atcaacctgt tgaagtgctg gctgccgacc tgcgccgtgc gttctccggt
    21061 attgtggcgg gtaacgtaaa agaagtcggt attcgcgcca ttgaagagtt tggtccttac
    21121 aaaatcaacg gcgataaaga gattatgcgt cgtatggacg acctgctaca gggttttgtt
    21181 gcccagcatc gtatgaagtt gccaggctca gcctacatcc cttgctacga aatctgcacg
    21241 taatctcccc tcc
//
LOCUS       DAJTSA010000068        20880 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000068 DAJTSA010000000
VERSION     DAJTSA010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20880
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.068"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..304
                     /locus_tag="OO369_002679"
     CDS             <1..304
                     /locus_tag="OO369_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCR5982905.1"
                     /translation="LIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMALS
                     STLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            276..2186
                     /locus_tag="OO369_002680"
     CDS             276..2186
                     /locus_tag="OO369_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR5982906.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLQFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTCDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TRVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGIFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVQLHPARMQLHNR
                     PLKNWQRSCPEE"
     gene            2187..2390
                     /locus_tag="OO369_002681"
     CDS             2187..2390
                     /locus_tag="OO369_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5982907.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2387..3980
                     /locus_tag="OO369_002682"
                     /pseudo
     CDS             2387..3980
                     /locus_tag="OO369_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3970..5475
                     /locus_tag="OO369_002683"
     CDS             3970..5475
                     /locus_tag="OO369_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR5982908.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5512..5859
                     /locus_tag="OO369_002684"
     CDS             5512..5859
                     /locus_tag="OO369_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR5982909.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5917..6945
                     /locus_tag="OO369_002685"
     CDS             5917..6945
                     /locus_tag="OO369_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR5982910.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6997..7383
                     /locus_tag="OO369_002686"
     CDS             6997..7383
                     /locus_tag="OO369_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5982911.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7395..7772
                     /locus_tag="OO369_002687"
     CDS             7395..7772
                     /locus_tag="OO369_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5982912.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7759..8343
                     /locus_tag="OO369_002688"
     CDS             7759..8343
                     /locus_tag="OO369_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5982913.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPMSGPL
                     TQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            8340..8741
                     /locus_tag="OO369_002689"
     CDS             8340..8741
                     /locus_tag="OO369_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5982914.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPAMKDIPVLSGLID
                     TMIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            8752..9492
                     /locus_tag="OO369_002690"
     CDS             8752..9492
                     /locus_tag="OO369_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR5982915.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            9551..9937
                     /locus_tag="OO369_002691"
     CDS             9551..9937
                     /locus_tag="OO369_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR5982916.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            9946..10275
                     /locus_tag="OO369_002692"
     CDS             9946..10275
                     /locus_tag="OO369_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR5982917.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            10247..13288
                     /locus_tag="OO369_002693"
     CDS             10247..13288
                     /locus_tag="OO369_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5982918.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTAFNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            13288..13617
                     /locus_tag="OO369_002694"
     CDS             13288..13617
                     /locus_tag="OO369_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5982919.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            13617..14315
                     /locus_tag="OO369_002695"
     CDS             13617..14315
                     /locus_tag="OO369_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5982920.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            14320..15063
                     /locus_tag="OO369_002696"
     CDS             14320..15063
                     /locus_tag="OO369_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5982921.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            15000..15602
                     /locus_tag="OO369_002697"
     CDS             15000..15602
                     /locus_tag="OO369_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR5982922.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            15663..19142
                     /locus_tag="OO369_002698"
     CDS             15663..19142
                     /locus_tag="OO369_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5982923.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            19210..19809
                     /locus_tag="OO369_002699"
     CDS             19210..19809
                     /locus_tag="OO369_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5982924.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            19873..>20880
                     /locus_tag="OO369_002700"
     CDS             19873..>20880
                     /locus_tag="OO369_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5982925.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLSEIAAAG
                     EKAQQKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPEDVIRFDTKSD
                     FLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYRAKSLTFYGI
                     NG"
BASE COUNT         4891 a         4947 c         6579 g         4463 t
ORIGIN      
        1 actcattgcc agatgggaac tgaccgcaga acaggctgtc gcacagcagt taaaaaatca
       61 ggtgtcaaaa ggaaatctta tcgataccgg attttgtatt tttgccctta gtaagctggc
      121 gatggcttta tccagtacgc ttgactccat tcctttgtcc atgcaacgac agttcccgga
      181 tttaacgccg cgtcatattg atcatctgaa aatccttatt gctaagggag cgaatcagtg
      241 cgcgcgtgcg ggggataaat taccggattt actcgatgaa tatatcagaa caacaactga
      301 gtaatatgat ggtggcagtc actatcgcac tgcagccact ggtcagggta ttgccggtga
      361 cggctgtaga atgggccgat caaaattatt acctgcctaa agaatcgtca tacggtgagg
      421 gggaatggaa aacgctgcag tttcaggtcg ccatcatgaa ctgcatgggc aatgacgaga
      481 ttcgtacagt taacctgatt aaatctgccc gtgttggtta tacaaaaatg gtgcttggtg
      541 tgatcgggta ttttattgag cacaaatccc gcaacactct tctttttcag ccgacggact
      601 cagctgctga agattttatg aagtctcacg tggaagcaac aattcgtgat gttccttgcc
      661 tgaaaaaact ttctccctgg ctgggccgga aacaccggga taatacgctc acactgaagc
      721 gtttttcctc tggtgtgggg ttctggtgtt tgggtggtgc tgctgccaaa aactaccgtg
      781 aaaaatctgt cgatgtggtc tgctatgacg aactctcctc gtttgaaccg gatgtggaaa
      841 aagaaggttc gccaacactg cttggcgata aacgtattga aggttcggta tggccaaaat
      901 ccatacgcgg ttcaacgcca aaaattaaag gctcctgtca gattgaaaaa gctgcgaatg
      961 aatctgcgca tttcatgcgg ttttatgtcc cttgccctca ttgcggggag gagcagtatc
     1021 tgaagtttgg cgatgatgcg acgtcgtttg gcctgaaatg ggagaagggt aaaccggaaa
     1081 cggtgtatta cctgtgtgaa cataatggct gcgtgatccg tcagtctgaa cttgatcaga
     1141 ccggggggcg ctggatttgt gacaataccg ggatgtggac gtgtgacggt ctgacgtttt
     1201 tcagtgcttc gggtaatgaa attccgccgc cgcgctccat cactttccat atatggacgg
     1261 cgtacagtcc gttcaccacc agggtacaga tagtctgtga ctggctggat gcactgaaag
     1321 atcccaacgg cgtgaaaact tttgtgaaca ccacgctggg cgagacctgg gaagaggccg
     1381 tgggcgaaaa actcgatcac caggtactga tggataaggt tgtgccttac acggcaacgg
     1441 tgcctgtccg ggtggtttat ctgacggcgg gcattgactc gcagcgaaac cgttttgaga
     1501 tgtatgtctg ggggtgggct ccgggagagg aagcctttct ggtggataaa atcatcatca
     1561 tgggacgtcc tgatgaggaa gagacgctgt tacgtgtgga tgcggcgatc aataaaaaat
     1621 accgccatgc agacggtacc gaaatgacca tttcccgtgt ctgctgggat atcgggggga
     1681 tcgatggcga aatcgtgtat cagaggtcaa aaaaacacgg tattttccgg gtactgccgg
     1741 taaaaggtgc ctctgtctat ggcaagccgg tgatcaccat gccgaaaacc cgcaatcagc
     1801 ggggtgtgta tctgtgtgag gtggggacgg ataccgcaaa agaaattctc tatgcccgaa
     1861 tgaaggccga tcccacccct gtgaatgagg ccacatcgta tgccatccgc tttcctgata
     1921 atccggagat tttttcgcag acagaggcac aacaactggt ggcggaggaa ctggtggaaa
     1981 agtgggaaaa aggaaagatg cgtctgttgt gggataacaa aaagcggcgt aacgaagcgc
     2041 tggactgcct ggtgtatgcc tatgcggcat tacgtgtgtc cgtgcaacgc tggcaacttg
     2101 atctggctgt gcagttgcat cccgcaagga tgcaactgca caaccgaccc ttgaagaact
     2161 ggcagcgaag ctgtccggag gagtgaatgg ttacagtcgc tgaactgcag gcgctgcgtc
     2221 aggcgcgcct tgatttatta accggtaaac gggttgtgtc ggtacagaaa gacggtcgca
     2281 ggatcgaata tacggcagcg tccctgaatg agcttaaccg tgcgatcaac gatgcggagt
     2341 cggttctggg tacgacccga cgccgccgac cgctgggagt gaggttatga aacgaacgcc
     2401 tgtcctgatt gatgtgaacg gcgttccgct tcgtgagagt ctcagctaca acggaggcgg
     2461 cgcaggattt ggcgggcaaa tggcagagtg gttaccaccg gcgcagagtg ccgatgcggc
     2521 cctgttgcct gcattgcgtc tggggaatgc ccgggcagat gatctggtgc gcaataacgg
     2581 tatcgcggct aatgcggtgg ccctgcataa ggatcatatc gtcgggcaca tgtttcttat
     2641 cagctaccgt ctgaactggc gttggctggg gatgcgggag accacggcaa aaagttttgt
     2701 cggggaggtg gaagcagcct ggtcggaata cgcagaagga atgtttggcg agatcgacgt
     2761 ggaagggaaa cgcacgttta cggaatttat ccgtgaaggg gtgggcgtgc atgcctttaa
     2821 cggcgaaatc tttgtgcagc cggtctggga tacggaaacc acgcagttat tccgtacgcg
     2881 ttttaaagcc gtgagtccga aacgggtgga cacgccagga cacggtatgg ggaaccactt
     2941 tctgcgggcc ggggttgagg tcgatcggta tgggcgggcg attgcgtacc atatctgtga
     3001 ggatgatttt ccgttctctg gtgccggacg ctgggagcgg atcccgcgtg agcttcccac
     3061 cgggcgtccg gccatgctgc atatttttga gccggtggag gacgggcaga cccgtggggc
     3121 taatcagttt tacagcgtaa tggagcggat gaagatgctg gactccctgc aggccacgca
     3181 gcttcagtcg gccattgtga aggcgatgta tgcagccaca atcgaaagtg aacttgatac
     3241 cgaaaaggca tttgaatata tcgcgggggc accgcaaggt cagcaggata atccgcttat
     3301 taatattctg gagaagttca cccgctggta tgacacgaat aacgtgacgc tgggcggtgt
     3361 taaaattccg cacctttttt cctggcgatg cgctgaacct gcagacagcg caggattcag
     3421 acaatggatt ttcggcgctt gaacaggcgc tgctgcggta tattgcagcc ggtcttggcg
     3481 tttcctacga acagttgtcc cgtgattact cgaaggtcag ttactcaagt gcccgcgcct
     3541 ccgccaatga gtcgtggcgc tattttatgg ggcggcgaaa atttattgcg gcccggctgg
     3601 ccacgcagat gttttcctgc tggctggaag aggcgcttct tcgggggatt attcgtccgc
     3661 cacgggcgcg ttttgatttt taccaggcgc gttcagcctg gtcacgggca gagtggatta
     3721 gctccggaag aatggccatt gacgggctca aggaggtcca ggaatcggtg atgcgcattg
     3781 aggccggact gagcacgtat gagaaagaac tggcactgat gggcgaagat tatcaggaca
     3841 ttttccgcca gcaggtcagg gaatccgcag agcgcgaaaa agccggactc tcacgtccgg
     3901 tgtggatagc ccaggcgtat cagcagcaga tagcggagag tcgcaggccg gaagaggaga
     3961 caacatcacg tgagacgtaa tctttcacat attatcgctg cggcattcaa tgagccgctg
     4021 cttctggagc ccgcctatgc gcgggttttc ttttgcgcgc tcgggcgtga gatgggcgca
     4081 gcaagccttt cggtaccgca acagcaggta cagcttgacg ctcccgggat gctggctgaa
     4141 acggacgagt acatggccgg aggcaaacga cctgcccgtg tttaccgggt ggtgaacggt
     4201 attgctgtac tgccggtgac cggcacgctg gtacaccggc tgggtggcat gcggccattt
     4261 tccggaatga ctggctatga cggcattgtc gcctgtcttc agcaggcaat ggcggacact
     4321 caggtacggg gcgtactgct ggacattgac agttctggcg ggcaggccac cggcgcgttt
     4381 gactgcgctg acatgattta ccgtctccgg cagctgaagc ctgtctgggc actgtgcaat
     4441 gacacggcct gttcagccgc catgttactg gcatcggcct gctcccgacg gctggttacc
     4501 cagacatccc gtatcggctc cattggggtg atgatgggcc atgtcagcta tgccggtcat
     4561 ctggcacagg ccggtgtgga tatcacgctg atttacgccg gatcgcacaa ggtggatggc
     4621 aatcagtttg aatccctgcc agcaaaggtt cgccaggaca tgcagcagcg cattgatgcg
     4681 gcgcgccgga tgtttgctga aaaagtggcg atgtataccg gtttatctgt ggatgcggtt
     4741 atgggaacag aggccgccgt ttttgaaggt cagtccggta ttgaggccga actggcggat
     4801 gaattaatca atgcgtcgga cgccatcagc gtgatggctg cgacgctgaa cacacacgat
     4861 acaggaggca ctatgccgca attaactgca acggaggctg ccgcgcagga gaaccagcga
     4921 gtgatgggga tcctgacgtg tcaggaagcg aaaggacgtg aacaactggc cacgatgctg
     4981 gcagggcaac agggtatgag cgttgagcag gcccgggcga ttctggccgt ggcggcaccg
     5041 caacaatctg gtgtatctgc gcagagtgaa gccgatcgca ttatggcatg tgaagaggcg
     5101 aaaggacgtg aacaactggc ggcaacgctg gcggcgatgc cggagatgac ggtggaaaaa
     5161 gcccgtccga ttctggctgc ttcaccgcag gcgaatgccg gaccctcact tcgtgatcag
     5221 attatggccc tggatgaggc aaaaggggca gaggcgcagg ctgaaaaact ggcggcctgt
     5281 ccggggatga ccgtggagaa tgcccgggct gtactggcgg cagcctcagg cagggcagaa
     5341 ccggtttctg catccacaac cgccatgttt gaacatttca tggcgaacca ttcaccggca
     5401 gcggtacagg gtggcgtgcc acagacgtca gcagacggtg atgcggacgt gaaaatgctc
     5461 atggccatgc catgaagtca gtgctgacca tcaatatgag gtttttacaa tatggtaacg
     5521 aaaaccatca ctgaacagcg tgcggaagta cgtatttttg ccggtaatga tccggcccat
     5581 accgccacag gctgcagcgg gatttcctcg gcaacaccgg ctctgacgcc cctgatgctg
     5641 gatgaggata ccgggaaact ggtggtctgg gacggacaga aggcaggcca tgtggttggc
     5701 atactggtac tgccgcttga aggtacagag acggtgctga cgtattacaa gtcggggacc
     5761 tttgcgacgg aggccatcca ctggcctgag agagtggata cacacaaaaa ggcgaatgcc
     5821 tttgccggca gtgccctgag tcacgcagcg ctgccgtaac attttatcag gtcactgcgg
     5881 tagcctgact gatttctgaa tgaaaggaac tgatttatgg gattgtttac gacccgccag
     5941 ttactcggtt ataccgaaca aaaagttaaa tttcgtgcgc tgtttctgga gctgtttttc
     6001 cgccgtacgg tgaatttcca taccgaagag gtaatgctgg acaaaattac cggaaaaacg
     6061 ccggtggcgg cctatgtctc cccggttgtt gaaggaaagg tgttgcgtca ccgtggcggt
     6121 gaaactcgtg tactgcgccc gggctacgtt aagccgaaac acgaatttaa ttaccagcag
     6181 gcggtggagc gtcttcccgg tgaagatccg gcccagctga acggcccggc ttaccgtcgc
     6241 ctgcgtatca tcaccgataa cctcaaacag gaagagcacg ccattgtcca ggtggaagag
     6301 atgcaggcgg tgaatgccgt gctgtatggc aaatacacca tggaaggaga gcagtttgaa
     6361 actgttgagg tcgattttgg ccggtcggtg ggaaataaca ttacgcaggc aggagggacg
     6421 gagtggagcg ctcaggaccg cgataccttt gatccgacgc atgatctgga tgcgtactgt
     6481 gattttgctt ccggcaccat caatattgcc attatggatg gtaaggtctg gcgtctgctg
     6541 aatggcttta agctgttccg cgaaaaactg gatacccgtc gtggctcaaa ttcacagctg
     6601 gaaacggcag tgaaagacct gggggcggtg gtgtccttca aagggtatta tggtgatctg
     6661 gcaatcgtgg tggcaaaaac gtcctatgtg gcagaggacg gtaccgaaaa acgttatctg
     6721 ccggacggca cactggtact gggaaatacg gccgcagagg gtattcgttg ctacggtgcc
     6781 attcaggatg cgcaggcatt gtctgaaggc gtggtggcgt cttcccgtta tccgaaacac
     6841 tggctgaccg tgggtgatcc ggcgcgtgaa ttcaccatga cgcagtccgc gccgctgatg
     6901 gtgctgccgg atccggatga gtttgtggtg gtgcaggtga aataatccgt gagcgggagc
     6961 taaatgctcc cgtttttcat tcaatcagga gcgggtatgg ctacgaaaga agaaaacctg
     7021 aaacgtcttt gtgagctggc tgagaggctg gggcgtgaac cggatgtctc cggtagtgcg
     7081 gcagatattg cgcagcgagt ggcggaactg gaggaagagc ttggagatgc gggggaggtc
     7141 gcagagccgg atacgtactc accatcgaaa gatgatccga ccgcgcataa aggcgagtca
     7201 gttcctgaac agccaggcat tgttaccgga gacgaaacgg ggcaggttac ggtggtggca
     7261 ctggcgacac tgcatacaaa atgtaatggt tcagtgatat ttgtttcacc gggaacgtcg
     7321 tttcgtgttt ctgctgaggt ggctgccagc atggcggcag gtggtctggc aaaaaggcag
     7381 taacaggaga tgtcgtggac ggtttcgata atctgtttga tgctgcgctt gccggtgtca
     7441 atgaagtcat tctccgtgat atggggatca gcgccgtcat tacgtccgga gaacttgagg
     7501 ggacacacct gacaggggtt tttgatgatc ccgaaagtat ttcttttgtt gccgggggga
     7561 tccggcttga ggattcctca ccgtgcctgt ttgtgaaaac ggcagatata tcgcagttac
     7621 gccgtcagga tacgttaact atcggggatg attctttctt tgtggatcgt attactccgg
     7681 atgacggggg atgctgttat atccggttaa ggcggggcag tcctgcccca cagaataatg
     7741 ccagaatgag gcgatatgat gaagggactt gagaatgcca tccgcaatct gaacagcctc
     7801 gatacccgca tggtgccaca gaccagcgca tgggcggtaa accgtgtggc ggcaaagatt
     7861 gtttctgtcg ccacacggca ggttgcgcag aataccgttg ccggggataa ccaggtgaaa
     7921 ggtattcccc tgaaaacggt cagggagcgt gtcaggctgc ttaaagccag cccctcagga
     7981 aaaatgtatg cccgcatgcg tgttaaccgg ggtaacctgc ccgccattaa gctgggtacc
     8041 gcacaggtca gactggcccg ctcccggcat ggcagcaact cacgtcatcg tggcagcgta
     8101 ctgaaagtgg ggaaatacct tttccgggat gcgtttattc agcaactggc gaatggtcgc
     8161 tggcatgtga tgcggcgtat tgacggcaaa aatcgttacc ccattgatgt ggtgaaaatc
     8221 ccgatgtccg gaccgctgac acaggcattt gaagatgccc gcgaccgcat cattgctgcg
     8281 gaaatgccga aacagctggg gtatgccctg aaacaacaac tgaggttaca tctgagtaaa
     8341 tgaaccgaca cacacaaatc cgtcagagcg tgctggcacg ccttcgggaa cagtgtggag
     8401 acagcgccac gttttttgac gggcttccgg catttattga tgcgcaggaa ctgcccgccg
     8461 tggcggtgtg gctgagtgat gcgcagtaca ccggaaaaat gacggatgag gacgactggc
     8521 aggctgttct gcatattgcc gttttcgtcc gtgcacaggc gccggattct gagctggata
     8581 cgtggatgga gaacaccata tttccggcca tgaaggatat tccggtgctt tccggactca
     8641 tcgacaccat gatcccactc ggttttaact atcaacgtga taatgagatg gccacctggg
     8701 cgatggcgga aatcacgtac cagatcacgt acacgaatta aggaggtggt aatgaccaca
     8761 ccaaatccac tggcaaagac gaaaggtgcg gggacgacgt tctggatgta taccggcaac
     8821 ggcgatgcgt ttgcgaaccc tttgtcggac actgactggc tgcgtcttgc gatggtgaag
     8881 gatctgcaac ctggcgaaat gaccgctgat gcagaagatg acacttatct cgatgatgaa
     8941 gatgcagact ggaaaacgac aacccagggg cagaaatccg ttggtgatac ttcggcgacg
     9001 ctggcctggc gtccgggtga cagcgggcag aaaaaactgg ttcagttgtt cgactccggt
     9061 gaagtctgcg cgtttcgtat caaatatccc aacggcactg ttgatgtttt ccgcggctgg
     9121 ctgagctcac tgggtaaaac cattgcctca aaagacgtga tgacccgcac tgtgaaaatc
     9181 agcggtgtgg ggcgtccgta tctggcagag gaaggcactg aaaccgtggg tgttaccgga
     9241 ctgacggtgg caccggcatc tgtcagtgtc aaagcgggag caaccaccac actgaccttt
     9301 acagtaaaac ctgacggggc cagtgacaaa gcgatcagtg tgcattcgtc agatcctcag
     9361 actgcttcgg tgaccctgag cgggcttgtg gccacggtga aaggcgtgaa gcagggcagt
     9421 gtcagcatcg tgggcatgac ctctgacgga gagtttgtgg cagtggctgc ggtgaccgtc
     9481 agcgcaccat aacaggacga tactcatcat tgccccggtt atccggggct ttttgcatcc
     9541 ggagaacatg atgtttctga aacaggatac gtttaattat gaaaaacagt ccgtggtgct
     9601 cagtgagctg tccgggctgc agagaattga atatctgacg tttgttcagc agcgaacggc
     9661 aaagtttgat gcacaggagg gagaactgcc ggaggctgaa cgacagattg cttttctgcg
     9721 tatgggaatg gatatcaatg cctggctggt ttcccgctca ctgtggaatg ctgagcagtc
     9781 tcaggatgtt gagacgcttt gcgcatccat tatgacaaca tggtcgtatg atgcgctggg
     9841 cgcgggggcg gagagggttc tgtcgctgag cggtatgggg accattgaga atgccgggga
     9901 tgatgatcat gaggcgctga cgccggaaaa gtcctgacgc gggaaatgca gtttgtcatg
     9961 cggcttgccc gggagttccg gcgggcagac tggcggcgga tgctgtcgga tatgtcggcc
    10021 actgagcttg gtgagtgggg cgattatttc cggatgcaga gcttcagtga tgtgtggatg
    10081 gatgcgcagt ttgcctcgct gaaggcattg atcgtgagaa tggtgtccgg cagcagcgat
    10141 gctgcggtgg ctgatttcag ccttttaccg gaagagaacg ggataccgga gcgaacggac
    10201 gaagaactga tgcatcttgg ggaaggtatt tccggaggtg tgcgttatgg accagatagc
    10261 caacctggtc attgatttgg ggattgatgc ggcagagttt aaaaatgaaa ttccccgtat
    10321 caaaaacctt ctgaatggtg cagccagcga tgcagaacgg tcttctgccc gtatgcagcg
    10381 ttttatggag cgtcagactc aggcggcccg gcagacaatg caggcggctt cttcggctgc
    10441 aacagcagca tcagcccatg cgcagacggt ggagaagaat gcacgggctc atgaacgcat
    10501 ggcccgtgag gtggaacaaa cccgtctgcg cgtggatgcc ctgaatcaga aaatgcgcga
    10561 ggaacaggcg caggccaggg cactggcgga ggcgcaggat aaagcggctg ccgcctttta
    10621 tcgccagatt gacagtgtga aacaggccgg tgcggggctt caggaattac agcgtattca
    10681 gcagcagatc cgacaggcca gaaacagtgg cggggttggt cagcaggatt atctggcgct
    10741 gatttcggag atcacggcga aaacccgtgc cctgacgcag gcagaggaac aggccacccg
    10801 gcagaaagcg gcgtttatcc gccagcttaa agagcaggca acccgccaga atctgtcgtc
    10861 ttctgagttg cttcgcgcca gggcggctca gctgggggta agcagtgctg cagaagtgta
    10921 tatccgcaaa atggagcggg caggaaaagc cacacattcg ctggggctga aaagtgcggc
    10981 agcccggcgg gagctggggg tgttaatcag tcagatggcg cgaggcaatt ttggtgcgct
    11041 gaggggatcc gggataacgc tggctaaccg tgccggatgg acaggcgcac tgatgtcgcc
    11101 gaaaggcatg atgactggcg gcgttattgg cggacttgtc gcggcggtcc tgggtctggg
    11161 taaagcctgg catgacggcc ggaaggaggg cgagaaattt aaccgtcagc tggcgctgac
    11221 gggacattat gccggtgtca ctgtcgggca gttgtggaaa ctcagccggg ccatatccgg
    11281 gaatggtatc acgcaacatg cggcagccgg tgcgctggca caggtggtgg gcagtggggc
    11341 atttcatgga aacgatattg gtatggtggc gaaagctgcc gcacagatgg agcgatcggt
    11401 tggccagtcg gtcagcgata ccataaatca gtttaagcgg ctgaaggatg atcctgtaaa
    11461 tgccgcgaag gcgctggaca atgcgctgca ttttttgaat gccactcagc ttgagcagat
    11521 acgcgtcctt ggggagcagg ggcggtccag tgatgcggcc cggatcgcca tgtctgcgct
    11581 ggcagaggaa accggtaaac gcacatctga tattgataat aatctcaatg cgctgggtag
    11641 tacgctgcaa accttgtctg actggtggaa gcagttctgg gatgcggcca tgaatattgg
    11701 tcgtgaagac tcgctggatg cgcagattga tgcgttacag gaaaaaattc agcgcgcgaa
    11761 aaaatatccg tggacaaacg cctccacaca ggtggagtac gatcagcagc gtcttaacga
    11821 tcttcaggag aaaaaacgcc ggaaggattt gcaggatgca aaagcgcagg cagaacggaa
    11881 ttaccaggag caacagaaac gccggaatgc tgaaaatgcc gcgctgaacc ggatgaatga
    11941 aacggaagct gcacgacatc agcgggaaat tgcgcgtatt aatgccatgc agtacgccga
    12001 ccaggctgtc agggatgcgg cgatacagcg tgaaaatgaa cgttacgaaa aagccattaa
    12061 gaaaaataca cgggcaaccc gtaatgatga ggccacccgg ttattgctgc agtacagtca
    12121 gcagcaggca caggtggaag ggcagattgc tgctgccaga cagtcagcag gcattgccac
    12181 tgaaaggatg actgaagcgc ataaacagct tctggccctg cagcagcgca tcagcgacct
    12241 ggacgggaaa aagctgacgg cagatgaaaa gagtgtgctg gcccgtaaga atgagctgat
    12301 tcaggcgctg acgctgctgg atgtgaaaca gcaggagctg cagaaacaga cagcgtttaa
    12361 cgacctgaga aaaaaaacgg ttcagctgac cagccagctg gcagacaaag aacgtgcact
    12421 gcgtgagcag cacaatctgg atattgccac tgcaggtatg ggggataagc agcggcagcg
    12481 ctaccaggca cagttgcgca tccggcagga ataccggcaa cagttgcaac agcttgagaa
    12541 tgacagtcgc cagaaaggca cttacgggac ggaggactac cggagggctg aggaggtgct
    12601 gaaggggagc ctgaagcgac aactgaatga aaacaaacgc tactggcagg aactggaagt
    12661 ggcgcagggc gactggaaaa acggtgccat gcgggcgttt cagaatttta cggcggatgc
    12721 ggataatgcg gcgggaacgg cagaacagat gtttacagtg gcattcagca gtgccggtaa
    12781 tgcactggcg acattctgta ccaccggtaa gctgaatttt aaatccttca cctcttccct
    12841 gttgtcagat atggccagaa ttatggcaca gatggccatg atgcaggcgg taaagggcgt
    12901 cggttcttta ttcggcttca cgactaatgc tgatggcggt gtttaccagt ctgctgattt
    12961 gagtcgctac agtggcacgg tggttaaccg tccgacgttt tttgcttttg caaaaggcgc
    13021 gggtgtgatg ggggaagcgg gacctgaagc cattctgcca ctgcgtcgcg gtgctgatgg
    13081 taagctgggg gttgtggcgg atactggtgg ttcaggcatg gtgatgtttg ccccgcagta
    13141 caacattgag atcaataacg acggcacgaa cgggcagata ggtccggctg ccctgaaggt
    13201 ggtttatgac ctcgggaaaa aagcggccgc ggactttatg caacagcagg cccgtgatgg
    13261 tggccggtta agtggagcat accggtaatg gagacgtttc actggaaagt gcgcccggat
    13321 atgaatgtgg tatcagagcc gaaagtggtg acagtgaagc tgggcgatgg ttatgaacag
    13381 cgtcgtgcgg cgggactgaa taaccagttg tcgacttaca gcgtgacgat acgtgttcgt
    13441 aaaggtgaac acccatcttt aaaagccttt ctggaacggc acggtggcgt ccgcgcattt
    13501 cagtggacgc caccttatga ctggaaactg atcagggtgg tttgtcgtaa atggtcggca
    13561 agcgtggggg cgttatgggt gactgtaacg gccgattttg aacaggtggt gaactgatgc
    13621 aggatatccg gcaggaaaca ctgaatgaat gcacccgtgc ggagcagtca gcccgggtgg
    13681 agctctggga aatcgatctg acagaggtcg gtggtgagcg ttattttttc tgtaatgagc
    13741 agaacgaaaa aggtgagccg gtcacctggc aggggcggca gtatcaggca taccccattc
    13801 aggggtcggg gtttgaactg aacggcaggg gctgtgctgc ccgtccgaca ctgacggtct
    13861 ctaacctgca cggcatggtc accgggatgg cggaagatct gcagagtctg gtcggcggaa
    13921 cggtggtccg gcgtaaggtt tacgcccgtt ttctggatgc ggtgaacttc gtcaacggaa
    13981 acagcgacgc cgatccggaa caggaggtga tcagccgctg gcgcatcgag cagtgcagcg
    14041 aactgagcgc ggtcagtgcc tcttttgtgc tggccacacc aacggagacg gatggcgcgg
    14101 ttttcccggg gcgtatcatg ctggcgaata cctgtatgtg gacttaccgt tctgatgagt
    14161 gtggttacac gggcagggca gtggctgacg agttcgacaa accaacaacg gatatccgga
    14221 aggacaaatg cagcaagtgt atgcgcgggt gtgagttgcg caacaatacc ggtaatttcg
    14281 gcggtttcct ttccatcaat aaactttctc agtaaatcca tgacacagac agaatcagcg
    14341 attctggcgc acgcccggcg atgtgcgcca gcggagtcgt gtggcttcgt ggtgagaacg
    14401 ccggagggag acaggtatct tcccagcgag aatatctccg gtgagccgga ggaacggttc
    14461 cggatggctc cggaggactg gctgcgggca caaatgcagg gtgagattgt ggcactggtc
    14521 cacagtcatc ccggtggtct gccctggctg agtgaggctg accggcggct gcaggtgcag
    14581 agtgatttgc cgtggtggct ggtctgccgg gggacgattc acaagttccg ctgtgtgccg
    14641 catctcaccg ggcggcgctt tgagcacggg gtgacggact gttacacgct gttccgggat
    14701 gcttatcatc tggcggggat tgagatgccg gattttcatc gcggggatga ctggtggcgt
    14761 cacggtcaga atctctatct ggataatctg gaggccacag ggctgtatca ggtgccgttg
    14821 tcatcagcac aaccgggcga tgtgctgctg tgctgttttg gttcatcggt gccgaatcat
    14881 gccgccattt actgcggcga tggtgagctg ctgcaccata ttcctgaaca actgagtaaa
    14941 cgagagaggt ataccgacaa atggcagcga cgcacacact ccctctggcg tcaccgggaa
    15001 tggcacgcat ctgcctttac ggggatttgc aacgatttgg ccgccgcatc gaccttcgtg
    15061 tgaaaacggg ggctgaagcc atccgcgcac tggccacaca gctcccggcg tttcgtcaga
    15121 aactgagtga cggctggtat caggtgcgca ttgccgggcg tgatgcaggt gaaaccgaat
    15181 tgtctgcccg tcttaatgag ccgctggcaa atggtgcagt gatccacata gtaccgcgtc
    15241 tggtgggagc taaaagtggc ggtgtgtttc aggcggtgct gggggcagct gttatggcgg
    15301 ttgctatatg gatgccgggg gtaggaatta tggcgagtaa tctgctgttt tctctcggtg
    15361 ccagtatgac gcttggcggt gttgcacaga tgctggcacc gaaagccaga actccccgta
    15421 cacagacaac ggataacggc aaacagaaca cctgtttctc ctcactggat aacatggttg
    15481 cccagggcaa tgttctgcct gttctgtacg gtgaaatgcg cgtggggtcg cgggtggtat
    15541 ctcaggagat cagcacggca gacgaagggg atggtggtca ggttgtggtg attggtcgct
    15601 gatgcaaaat gtttcatgtg aaaccgcctg cgggcggttt tgtcgtttat ggagcgtgag
    15661 gaatgggtaa aggcagcagt aaggggcata ccccgcgcga agcgaaggac aacctgaaat
    15721 ccacgcagtt gctgagtgtg attgatgcca tcagcgaagg gccggttgac ggtccggtgg
    15781 atggattaaa aagcgtgctg ctgaacggta cgccggtcct ggatagcgag gggaagacaa
    15841 acttttccgg tgttacggtg gtgttccgcg ccggcgagca ggagcagaca ccgccggagg
    15901 ggtttgaatc ttccggctca gagactgtgc tgggtacgga agtgaaatac gacacgccga
    15961 tcacccggac catcacgtcg gcaaacattg atcgtctgcg cctgaccttc ggtgtgcagg
    16021 cactggtgga aaccacctca aagggggacc ggaatccgtc ggaagtccgc ctgctggttc
    16081 agatacagcg taacggtggc tgggtgacgg aaaaagacat caccattaag ggcaaaacca
    16141 cttcgcagta tctggcctcg gtggtggtgg gtaacctgcc gccgcgcccg tttaatatcc
    16201 ggatgcgcag gatgacaccg gacagcacca cagaccagct gcagaacaaa acgctctggt
    16261 cgtcgtacac cgaaatcatc gatgtgaaac agtgctaccc gaacacggcg ctggtcggcg
    16321 tgcaggtgga ttcagagcag ttcggtaacc agcaggtgag tcgcaattat catcttcgcg
    16381 ggcgcattct gcaggtgccg tcgaactata acccgcagac gcggcaatac agcggtatct
    16441 gggacggaac gcttaagcca gcatacagca acaacatggc ctggtgtctg tgggacatgc
    16501 tcactcatcc gcgctacggc atggggaaac gtcttggtgc ggcagatgtg gacaaatggg
    16561 cgctgtatgt catcggccag tactgcgacc agtcagtgcc ggacggattt ggcggcacgg
    16621 agccgcgcat cacctgtaac gcttacctga ccacacagcg taaggcgtgg gatgttctca
    16681 gtgatttctg ctcggcgatg cgctgtatgc cggtatggaa cgggcagacg ctgacgttcg
    16741 tgcaggaccg accgtcggat aaagtgtgga cctataaccg cagtaatgtg gtgatgccgg
    16801 atgatggcgc gccgttccgc tacagcttca gcgccctgaa ggaccgccat aatgtcgttg
    16861 aggtgaactg gattgatccg gacaacggtc atgagacggc gacagagctt gtggaggaca
    16921 cgcaggccat tgtccgttac ggtcgtaacg tcacgaagat ggatgccttt ggctgtacca
    16981 gccggggaca ggcgcaccgc gccgggctgt ggctgattaa aacggaactg ctggaaacgc
    17041 agaccgtgga cttcagcgtc ggcgcagaag ggcttcgcca tgtaccgggt gatgtcattg
    17101 aaatctgcga tgatgattat gccggtatca gcaccggtgg tcgcgtactg gcggtgaaca
    17161 gccagacccg gacgctgacg ctcgaccgtg aaatcacgct gccgtcctcc ggtaccacgc
    17221 tgataagcct ggttgacgga aatggcaatc cggtcagcgt ggaggttcag tccgtcaccg
    17281 acggcgtgaa ggtgaaagtg agccgggttc ctgacggcgt tgcagaatac agcgtgtggg
    17341 ggctgaagct gccgacgctg cgccagcgcc tgttccgctg cgtgagtatc cgtgagaacg
    17401 acgacggcac gtatgccatc accgccgtgc agcatgtgcc ggaaaaagag gccatcgtgg
    17461 ataacggggc acactttgac ggtgaccggc gcggcacggt gaatggtgtc acgccgccag
    17521 cggtgcagca tctgaccgcc gaagtcaccg cagacagcgg ggaatatcag gtgctggcgc
    17581 gatgggacac gccgaaggtg gtgaagggtg tgagcttcct gcttcgcctg accgtggcag
    17641 cggacgacgg cagtgagcgg ctggtcagca cagccaggac gacggaaacc acataccgct
    17701 tcaggcagct ggcgctgggg aattacagtc tgacagtccg ggcggtaaat gcccgggggc
    17761 agcagggcga tccggcgtcg gtatcgttcc ggattgcggc accggcagcg cctgtcacta
    17821 ttgaactgat accggggtat tttcagataa cggtggtccc gaaactggct gtatatgacc
    17881 cgacggtgca gtttgagttc tggttttcgg aaaagcggat tgcggatatc aggcaggttg
    17941 aaaccagcgc ccgctatctt ggcacggcgc tgtactggat agccgccagt atcaatatca
    18001 ggccgggcca tgattattat ttttacgttc gcagtgtgaa caccgttggc aaatcggcat
    18061 ttgtggaggc cgtcggtcgg gcgagcgatg atgcggaagg ttatctgagt ttttataaag
    18121 ggttgatcaa taaaacgcat ctcggcaagg agttgtggac gcagattgat aacggtcagc
    18181 ttgcgccgga cctgactgaa atcaggacgt ccattacgaa tgtcagcaat gaaatcacgc
    18241 aaaccgtcaa taaaaaactg gaaaatcaga gtgctgcaat ccagcagata cagaaagttc
    18301 aggttgatac aaataataac ctgaacagca tgtgggctgt gaagctgcaa cagatgaagg
    18361 acggacgcct ttatattgcg ggtatcggtg ccggtattga gaatacgcca gcaggaatgc
    18421 agagtcaggt gctgctggcg gcagacagga ttgcgatgat taatcctgcg aatggcaaca
    18481 caaagccgat gtttgttggt cagggcgatc agatattcat gaacgacgtg ttcctgaaac
    18541 gcctgacggc tccgaccatt accagcggcg gtaatcctcc ggcattttcc ctgacacctg
    18601 gcggacggct gacggcgaaa aatgccgata tcagcggtaa cgtgaacgcg aactccggga
    18661 cgctcaacaa cgtcacgatt aataagaact gtcgggttct gggaaaattg tccgcgaacc
    18721 agattgaagg cgatctcgtt aaaacagtgg gcaaaccttt cccacgggac tcccgggcac
    18781 cggagaggtg gccatcaggg accattaccg tcagggttta tgacgatcag ccgtttgatc
    18841 ggcaaattgt tattcccgcg gtggcgtttc gcggtgctaa acatgagcgg aagaataaca
    18901 atatttattc gtcatgccgc ctgatagtga agaaaaacgg tgctgaaatt tataaccgaa
    18961 cgaccctgga taatacgctg atatatacgg gtgttattga tatgcctgcc ggtcacggtc
    19021 acatgacgct ggagttttct gtatcggcat ggctggtaaa tggctggtat cccacagcaa
    19081 gtatcagcga tttgctggtt gttgtgatga agaaatccac agcaggtatc agtatcagct
    19141 gaattttata acccagatac gggcaccaga aatggtgcct tttttattgc agaaaagcga
    19201 gaggtaatta tgcgtaaact ttatgctgcc attttgtccg cagccatctg tctggctgta
    19261 tccggtgcgc ctgcatgggc gtctgaacat cagtccacgc tgagcgcggg gtatcttcat
    19321 gcctcgacga acgctcccgg cagtgatgat ctgaacggga ttaacgtgaa ataccgttat
    19381 gagtttacgg acacgctggg gctggtgacg tcattcagct atgccaacgc tgaagatgag
    19441 caaaaaacgc attacagtga tacccgctgg catgaggatt ccgtgcgtaa ccgctggttc
    19501 agcgtgatgg cggggctgtc tgtgcgcgtg aatgaatggt tcagcgcgta tgcgatggcg
    19561 ggcgtggctt acagccgtgt gtcgaccttc tccggggatt atctccgcgt aactgacaac
    19621 aaggggaaaa cgcacgatgt gctgaccggg agtgatgaca accgccacag caacacgtct
    19681 ctggcgtggg gggctggcgt gcagtttaac ccgaccgaat ccgtggccat tgaccttgct
    19741 tatgaaggtt ccggcagtgg cgactggcgc actgacggtt tcatcgtggg tgtcggttat
    19801 aagttctgat tagccaggta acacagtgtt atgacagccc gctggttcag gcgggctttt
    19861 tgtggggtga atatggcagt aaagatttca ggtgtactga aagatggggc gggtaaacct
    19921 gtcgtaaatt gtgcgattga actgcgggcc agaagaacca gtccgaccgt tgtggcacac
    19981 gttgttgcca cttgcgtgac ggacaataac ggtgcttatg tgattgaggc tgagccgggg
    20041 tattacgagg ttgcgcttca ctgtaacggc tggcagccaa cccgtgtcgg ggatattgat
    20101 gtggcaccga ctgatgcacc ggggacactg aacgcgtttc tgaatgcacc aaaggatggt
    20161 gatttacgtc cggaggtgat gaagcgcttt gaggaaatgg tggcgcaggc gcagcagagt
    20221 gccggggctg cagccggaaa cgcacagcag acggcgcagg atgtggcggc agccgcaacg
    20281 gcccgtgatg atgcacaacg ttttgcggag aaagcccgac aggatgcaac cgtcacagct
    20341 gaggacagaa aggccactgc ggaagatgtg acaagcacag gagcaaatgc agccgcagcc
    20401 ggacagagcg cacaggatgc cgcaggttat gcccgcgcag cagaacaggc caaaaatgac
    20461 attgatgctg cgctgaccgg cactctgaaa atggctaacc atctgtcaga aatcgcagca
    20521 gcaggcgaaa aggcacaaca gaagtcccgg gataatctgg ggctgaaaag tgcggccacg
    20581 atggaagcac agagcgacat ttacgaccgg acaaaaggcc gtctggcgat acccggcgca
    20641 ttcggctttg ggtgtgcttt tctgcctgaa gatgttatcc gttttgacac taagagtgat
    20701 ttcctggcct gggtaaggaa tgcgctgcca ggtgaatatt ccgttgctgg cccctacggc
    20761 atcatcatac ccgacacacg gtttgaaggg gtgctcagca tccggtggac tgatgcacgc
    20821 cctgagacaa cagaaccgcg gtacagagcc aaatccctta ctttttacgg cattaacggc
//
LOCUS       DAJTSA010000069        20779 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000069 DAJTSA010000000
VERSION     DAJTSA010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.069"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..579
                     /locus_tag="OO369_002701"
     CDS             <1..579
                     /locus_tag="OO369_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5982926.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="OO369_002702"
     CDS             597..1235
                     /locus_tag="OO369_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5982927.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="OO369_002703"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="OO369_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR5982928.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="OO369_002704"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="OO369_002704"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR5982929.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="OO369_002705"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="OO369_002705"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR5982930.1"
                     /translation="MSQTVHFQGNPVTVANSIPQVGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="OO369_002706"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="OO369_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR5982931.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="OO369_002707"
     CDS             complement(5552..6613)
                     /locus_tag="OO369_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR5982932.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="OO369_002708"
     CDS             complement(6610..8007)
                     /locus_tag="OO369_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR5982933.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="OO369_002709"
     CDS             8163..9161
                     /locus_tag="OO369_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5982934.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="OO369_002710"
                     /pseudo
     CDS             complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="OO369_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10217..11298)
                     /locus_tag="OO369_002711"
                     /pseudo
     CDS             complement(10217..11298)
                     /locus_tag="OO369_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="OO369_002712"
     CDS             complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="OO369_002712"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR5982935.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="OO369_002713"
     CDS             complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="OO369_002713"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR5982936.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14232..14855)
                     /locus_tag="OO369_002714"
     CDS             complement(14232..14855)
                     /locus_tag="OO369_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5982937.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(14852..15274)
                     /locus_tag="OO369_002715"
     CDS             complement(14852..15274)
                     /locus_tag="OO369_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5982938.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSHLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(15296..16071)
                     /locus_tag="OO369_002716"
                     /pseudo
     CDS             complement(15296..16071)
                     /locus_tag="OO369_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(16089..17141)
                     /locus_tag="OO369_002717"
     CDS             complement(16089..17141)
                     /locus_tag="OO369_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR5982939.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17172..18014)
                     /locus_tag="OO369_002718"
     CDS             complement(17172..18014)
                     /locus_tag="OO369_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR5982940.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(18001..18882)
                     /locus_tag="OO369_002719"
     CDS             complement(18001..18882)
                     /locus_tag="OO369_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR5982941.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18903..20186)
                     /locus_tag="OO369_002720"
                     /pseudo
     CDS             complement(18903..20186)
                     /locus_tag="OO369_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(20200..>20779)
                     /gene="ycjM"
                     /locus_tag="OO369_002721"
     CDS             complement(20200..>20779)
                     /gene="ycjM"
                     /locus_tag="OO369_002721"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCR5982942.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5070 a         5667 c         5053 g         4989 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggtgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaagcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagacggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa
    10201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    10261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    10321 cacggaccac taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    10381 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    10441 ttccgggcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    10501 taatttctct tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    10561 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    10621 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    10681 cacaatccgc gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    10741 ggtgttcagt ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc
    10801 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    10861 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
    10921 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    10981 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    11041 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
    11101 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    11161 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
    11221 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    11281 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    11341 gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac
    11401 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    11461 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
    11521 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac
    11581 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg
    11641 gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa
    11701 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc
    11761 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    11821 catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt
    11881 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    11941 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
    12001 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
    12061 ctgaccgtcc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    12121 gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    12181 ctcaagtaac gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
    12241 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
    12301 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    12361 cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    12421 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc
    12481 tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    12541 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc
    12601 cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg
    12661 aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc
    12721 actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata
    12781 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    12841 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
    12901 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
    12961 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    13021 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
    13081 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
    13141 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    13201 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    13261 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat
    13321 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
    13381 cgtgatacgg cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg
    13441 gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc
    13501 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    13561 ctcgcctgca tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata
    13621 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
    13681 atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc
    13741 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
    13801 tgacgcgtcg gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc
    13861 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
    13921 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
    13981 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac
    14041 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    14101 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    14161 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    14221 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
    14281 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    14341 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
    14401 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    14461 gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt
    14521 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    14581 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
    14641 gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca
    14701 tacatcgcag catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt
    14761 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
    14821 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    14881 agcgcaaacg atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc
    14941 acatttccca cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc
    15001 ctgcttccag tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa
    15061 ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc
    15121 cgtatttttc tgccagcgcc tgcgcctggg aaaggtggct gtcacagaca gccaccagtt
    15181 ccagatcgtt gcgggtgcag tagtacgaag catgaacctt atccgccacc tggcccgcgc
    15241 ctattatcgc gacccgcagc ggagagcttg tcattgcact tttcacaaag acctcttagc
    15301 aggtacgcaa ccaggccaac gaatcacggt acgcctgggc aggatcttct gcccggatac
    15361 gcccttcata caccacataa ccctgatagt tatcggcgcg cagctgttca aacagcgcgt
    15421 ggaaatcgag ggtgccactg cccggctggt aacgatggtt atcggcaata tgcacatgac
    15481 cgagcaagtc acggttgtcg tgcagcgcct gcgccaggtt atcttcttcg atgttcatgt
    15541 gatagaaatc gccgataatc tgcacatgtt taagattgtt ttcgacgatg taacgacgag
    15601 catcggcgag ggtgttgatc atatgatcct gatagcggtt taacggttcg agatacacca
    15661 cggttccggt acgcgcggcg acctgttcca gtacgcgcag ggaatcactc accagtttgc
    15721 ggtcgccgtc caggctacgc ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc
    15781 acgctgccgg aaccccccca cttctgccag cgcctcgaga atgcgttcga tctgctttaa
    15841 gccattaaga cgacgctctt cgataaagtc accaatccag ccgtcatagc cgccgcaggc
    15901 ggtggtcact ggtaaaccgg tttctttgat cgccgctttg acttcttcga tgttgttgac
    15961 cagcagtttg ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaatct
    16021 ctcgcggata ttttccggaa agaatgcctg attctgtgtg ccgattttca ttttttcatt
    16081 cctttgaatt aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac
    16141 tgcatgtagc tttccgggct gttggcaaag gtcactaccg ggtcgattaa atcttcgcaa
    16201 ttgagataac cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag
    16261 cgcggataat ccgggttcgg ttcgctgcac gcgcgggaga aaacaatttt ggcgttattg
    16321 aaatgcgctt cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcgacatag
    16381 gagatggtgc cgccgtaggc caggccgcgc agcgccgatt gcagcgcgtc ggcgtaaccg
    16441 ctggtttcga taatcacatc agccccctgc ttgccggtca gcgttttgat ctctttgcct
    16501 acgtcagtgc caatggggtt aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag
    16561 cgatgggcga taggatcgac gccaattacc accgatgcgc cagcgcgttt cgccagttgg
    16621 atggcaattt gaccgatcgc gccaagccct accaccacga caaaatcccc tacgcgtacg
    16681 ttggcatcgc gcacgccgct catggcaaac tgcgccgggt cgtaacagac ggcgtttttc
    16741 caggagctgc cttgcggcat tttgcgcagc ttgtagttat tcactgcgtt aatgatgacc
    16801 gtctcggaga gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc tgtaacgtcg
    16861 ctgccgcact cgataatgtc gccaaccacc atgttgccaa gctggaattt gccaaactca
    16921 atgccgcgcg gcgcatctgc cggacgcggg gtgaacatct gccactcgcc gttaaagtct
    16981 tcatcgataa acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg
    17041 ccgaaacgca cgcggatctt cacttcattg gctaaaatcg cccgatcttc atactcaacc
    17101 agcgcagcaa cacgcggtgc tgtggctact aactttttca tgtaaaactc cttgtaaaga
    17161 aactggccgc tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc
    17221 aaaagcgcat acataatcac caccggcaat gcggtcacca gcgaggccgc catcatccgt
    17281 ccccagatgt aatcaggcgt actgaacagc gcgttcaggc ccactggtaa ggtgaaattg
    17341 ctggcgctgg agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag
    17401 tagacgaaca ccgatatcag cccggacatc gccaggggca cggtgatacg gaagatgatt
    17461 tgcagacggt tgaggccatc catcatcgcc gcttcttcga tctcatcagg gatggtgtcg
    17521 aagtaacttt tcagcataaa cacggcggtg ggcagggttt gcgtcaccat ggtaatgatc
    17581 agcgccatct cggtgtcgta aataccaagc gcggtgatga ttttgaacag aggcaccacc
    17641 agcaaaatgc cagagaacat atacaccgtg taaaagctgg cgttgatggt catccggcct
    17701 ttaaagcgca ggcgggaaag cgcgtaagct ccgagaatgc cgagaaatac cgccaccacg
    17761 gaggaaacca ccgacaccac catgctgtta cggaagtagt cgacaaacgg gaaaatcatc
    17821 gggttaaaaa tgtcgacgta atgctccagc gtccactgtt gcggcagcag cgtaggatgc
    17881 agcgatatcg cctctttcgc gcttttgaac gaggtcatca gcatcacaaa aaacggaaac
    17941 agcgtgatga tgagaaacag cgccagcccg cagtaaaaac cgatacgact gagcgtgcgt
    18001 ttatttgttg ccattgaggt tcacccgttt cctggtcagc agaatgacag cgaaaataat
    18061 gatgaagagc accaccgaga tcgccgccgc tttccctaag tcattaaagg caaaggcggt
    18121 tttgtagaga tagacaccaa gaatatcgac cttggttgtc agcagataaa catcagcgaa
    18181 catgtagaac atccagatgg tgcgcagggt caccaccgtc gccaggaccg gcataattgc
    18241 gggcagcgtg acgatgcgaa accgctgcca ggcattagcg ccatccatct ctgccgcttc
    18301 gtacagcgat ttgtcgatgg tctgcaaaat cgccagaaac gagataaagg catacgggaa
    18361 gtagcgccag atggcgaaca gcaccaccag cacaaagcta ctgcccggat tatcaaacca
    18421 cagcggtgcc tgctcataga gatgcagaag atcgacgccg aggtagttca caatgccgta
    18481 gccgttgttg aacatgtatt tccaggcaaa caccagcgaa atggacggcg ttacgtagga
    18541 gaggatcacc agcgaacgcg cggttttgcg caggcggaac tcgcggttga aaaacatcgc
    18601 cacggccagc ccgagcacgg tgctgcccgc caccaccagc gccgtatacc agaccgtcat
    18661 ccacagcgag tgccagaagc cgggatcgga gagaatgcgc acatagttac tcaccccaac
    18721 aaacgttgac tcgatgttgg gattgagcgg cagacgtaaa aaactgattt cgatattcga
    18781 caccatcggc cacgccacca gaccgcccag cagtaataag ctgggcgcga gaagcagcag
    18841 cgcaaacggc atatcggaac gacctgaaaa aagcctgttc attgcggatt cctgtcctgg
    18901 gtttagtgct gttcgatcag ttcatccagc tttttctggc tcgcttgcag cgtgccaggg
    18961 agatcggctt tacccacggt gacgttatgc accatggagc tcaccacacc agaacccgtc
    19021 acatcgccca tgcgggtaaa gtttttatcc cctactgcgc caaagacctg aatatttggc
    19081 agttcaccga ttagctgatt cggtagttca cccagcgcct taataacgtc gttgtctttc
    19141 caggtggcgg tagtgacaac cgctttattc accggcagcg cagcacctgg cgacatcatc
    19201 acccagtcgg caatgttgtc cgcctgctcc ataaaggtga caaatttttc tgctgcttcg
    19261 gtcttttgtc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc
    19321 tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac agccggaagg
    19381 atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc
    19441 atgatgtcgt tagaacccgg catggtgttg gcagcaaggt cgcggtaata ggtcagcgcc
    19501 tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg cgttaaagac gttggcctag
    19561 ttggataacg caaactggga gaaggattgt tccgtcagca cgctttctgc cgtaggcagc
    19621 gcaataccgt attttttatt cgccgggtca ttcagtttct gtgcaacgtc cagcagctgt
    19681 tgccagtttt tcggctcctc cagccccgct tttgccagca catctttgcg ataccagatg
    19741 ccgccaatcc aggcgctgac aggaacaccg gtccatgcgc taccatcttc ggtacgcaca
    19801 atacgcagta cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac cgtggcaacc
    19861 gctttgcgat cgataagctg ctctttatcc ataactttgg cgtagtcatg gctggtttcg
    19921 atcacttccg gcagcgagcc gctacgtgaa agggtgatga ctttggtgtt atgagcgtct
    19981 tcttccacgg gcacttgttt aacggtgata cctgggtttt ctttttcaaa acgggcaatc
    20041 aatttgctga taactgcctg acgctcctgc tccaccgaag aatgcataaa ttcgatggat
    20101 acatgggttt tattttcttc tttacagcct gaaatcaggg cgcaggaaac cagtgctgat
    20161 aacagcacga tttttgattt aatcatgaga tgttcctttt tatttaatcc acataacctg
    20221 ccaggggcgc agcgttattt cattacccaa tatatcaact tcactaatca gatcccgacc
    20281 atgcagatca gtaatattta catgctgaat atttttactg acattaaaca atccagtcag
    20341 acaattacca tcagcgttac ttctttgaat acgcattacg gatgaattaa ccgtatcgat
    20401 ggtaaaatta ttatccggat gaaactcgtt atggctgcga cgaagtgtaa ttaaacgcga
    20461 caactcatga tataccgcat gccttaatgt agcttcatcg ttcagttctc gtgttatctc
    20521 ttcactgtga tattttttac ggttaatcgc acggttatat ccgagttttt cgacacctgc
    20581 gtaatcatta cgcgagccaa gaatactttg aatatatatc gcgggtacac cggggaaact
    20641 taacaaaatc gcatgggcaa ggataaacct ggtgcaacgt tcttcatcgc tactctcgcg
    20701 gcggcttaac gcattcatat aggtgacatt catctcatag ggactgcgcg tgccgtcggg
    20761 attatttttc cagtttact
//
LOCUS       DAJTSA010000070        20712 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000070 DAJTSA010000000
VERSION     DAJTSA010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.070"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="OO369_002722"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="OO369_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR5982943.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="OO369_002723"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="OO369_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR5982944.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="OO369_002724"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="OO369_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR5982945.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="OO369_002725"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="OO369_002725"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR5982946.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="OO369_002726"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="OO369_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR5982947.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="OO369_002727"
     CDS             complement(8217..9110)
                     /locus_tag="OO369_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR5982948.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="OO369_002728"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="OO369_002728"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR5982949.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="OO369_002729"
     CDS             complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="OO369_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR5982950.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /locus_tag="OO369_002730"
     CDS             complement(10241..11473)
                     /locus_tag="OO369_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR5982951.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="OO369_002731"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="OO369_002731"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR5982952.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="OO369_002732"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="OO369_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR5982953.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16537)
                     /locus_tag="OO369_002733"
                     /pseudo
     CDS             complement(14278..16537)
                     /locus_tag="OO369_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="OO369_002734"
     CDS             complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="OO369_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR5982954.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="OO369_002735"
     CDS             complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="OO369_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR5982955.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="OO369_002736"
     CDS             complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="OO369_002736"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR5982956.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="OO369_002737"
     CDS             complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="OO369_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR5982957.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4981 a         5727 c         5032 g         4972 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttccta gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcaccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcatcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaacg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccaggccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatccgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcgatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgcc cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggccatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagt cctgtgtcat agagaatcgc tttgccgttt ctggctatca
    15001 ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt
    15061 tttgccacag cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc
    15121 gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat agccattgcc
    15181 aacattcaac aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
    15241 ccaaagaccg gtcggcaaga aaccataacc cctgttccag gattaacggc ccgctaaaat
    15301 gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa
    15361 atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca
    15421 taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg
    15481 gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
    15541 cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
    15601 ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt aacttaagca
    15661 ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc
    15721 aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc
    15781 gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag
    15841 cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt
    15901 tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc
    15961 acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
    16021 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg
    16081 cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga
    16141 gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg ccatacagca
    16201 caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag
    16261 tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
    16321 tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg
    16381 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag
    16441 tcagaaacgc aagggttaat gtcctaggca attggggcaa aatcagcaac ggaaaaattc
    16501 cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa
    16561 gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga ggcacattca
    16621 gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct
    16681 gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa
    16741 aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac
    16801 gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc
    16861 gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag
    16921 agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat
    16981 gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt
    17041 tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc
    17101 ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg
    17161 gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc
    17221 cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt
    17281 ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac
    17341 gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac
    17401 gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac
    17461 ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt
    17521 cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg
    17581 ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc
    17641 acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc
    17701 gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc
    17761 aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg
    17821 tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac
    17881 tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc
    17941 ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt
    18001 caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac
    18061 ccacggatct tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa
    18121 cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac
    18181 gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa
    18241 tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt
    18301 ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac
    18361 aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc
    18421 caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg
    18481 aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac
    18541 agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt
    18601 ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac
    18661 ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct cagccgggat
    18721 ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac
    18781 aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag
    18841 ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc
    18901 catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca
    18961 aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc
    19021 aattgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt
    19081 ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc
    19141 tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct
    19201 tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg
    19261 tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat
    19321 gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta
    19381 cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac
    19441 acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca
    19501 acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga
    19561 tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg
    19621 ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac
    19681 cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca
    19741 aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag
    19801 aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat cacggatgtg
    19861 ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc gaccgccatc
    19921 caggctagcc agtttttcaa agctgccaac cagcccctgg gtgctgatgc cacgtttctt
    19981 caacagatca aaagagaaat catctgcttc ggactcttta tcacgggagt atttagcatt
    20041 aatagcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag ccacaccgct
    20101 ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaagctttca tttcagccag
    20161 cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa tttcgttgtc
    20221 attcatcatg tccatcaggc cactgtagac acgaacacag ccgttcgcca tcgcccatgc
    20281 gttgacgtcg ctggtcatat aaaccttata gttgaccggc gtgccattaa tattgttacc
    20341 caatgctttg gcgatttttg ccagacgttt accgtacttg ctggatttac ttgcgacttg
    20401 attgccgctg tccatttcgg cacagccctg attggcaatc gcttttgcat cggcatcgct
    20461 taatgttgct gctttatagg catttaaacc ggagctgata gccatattgg tatcaatacc
    20521 gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc gcaatcgcca gcagtaattt
    20581 agtattcttc atgtgaatca tccgttgatt aatcatatat aaatattatg tatcaacaac
    20641 ttacattgac aagcgacgct accattaatc aaaatgactc acaaggtccg aaaaaaaacg
    20701 ccgacaatgt gc
//
LOCUS       DAJTSA010000071        20691 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000071 DAJTSA010000000
VERSION     DAJTSA010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.071"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            81..350
                     /locus_tag="OO369_002738"
     CDS             81..350
                     /locus_tag="OO369_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCR5982958.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            344..709
                     /locus_tag="OO369_002739"
     CDS             344..709
                     /locus_tag="OO369_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR5982959.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(710..1498)
                     /gene="bioH"
                     /locus_tag="OO369_002740"
     CDS             complement(710..1498)
                     /gene="bioH"
                     /locus_tag="OO369_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR5982960.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1536..2219
                     /gene="gntX"
                     /locus_tag="OO369_002741"
     CDS             1536..2219
                     /gene="gntX"
                     /locus_tag="OO369_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR5982961.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2278..2853
                     /gene="nfuA"
                     /locus_tag="OO369_002742"
     CDS             2278..2853
                     /gene="nfuA"
                     /locus_tag="OO369_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR5982962.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3214..4530
                     /gene="gntT"
                     /locus_tag="OO369_002743"
     CDS             3214..4530
                     /gene="gntT"
                     /locus_tag="OO369_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5982963.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="OO369_002744"
     CDS             complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="OO369_002744"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR5982964.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6669..9062)
                     /gene="malP"
                     /locus_tag="OO369_002745"
     CDS             complement(6669..9062)
                     /gene="malP"
                     /locus_tag="OO369_002745"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR5982965.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9674..12379
                     /gene="malT"
                     /locus_tag="OO369_002746"
                     /pseudo
     CDS             9674..12379
                     /gene="malT"
                     /locus_tag="OO369_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="OO369_002747"
     CDS             complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="OO369_002747"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR5982966.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="OO369_002748"
     CDS             complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="OO369_002748"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR5982967.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14849..16447
                     /gene="rtcR"
                     /locus_tag="OO369_002749"
     CDS             14849..16447
                     /gene="rtcR"
                     /locus_tag="OO369_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR5982968.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16429..17187)
                     /locus_tag="OO369_002750"
     CDS             complement(16429..17187)
                     /locus_tag="OO369_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR5982969.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17204..18034)
                     /gene="glpG"
                     /locus_tag="OO369_002751"
     CDS             complement(17204..18034)
                     /gene="glpG"
                     /locus_tag="OO369_002751"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR5982970.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18079..18405)
                     /gene="glpE"
                     /locus_tag="OO369_002752"
     CDS             complement(18079..18405)
                     /gene="glpE"
                     /locus_tag="OO369_002752"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR5982971.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18595..20100
                     /gene="glpD"
                     /locus_tag="OO369_002753"
     CDS             18595..20100
                     /gene="glpD"
                     /locus_tag="OO369_002753"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR5982972.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20369..20599)
                     /locus_tag="OO369_002754"
                     /pseudo
     CDS             complement(20369..20599)
                     /locus_tag="OO369_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         4889 a         5693 c         5217 g         4892 t
ORIGIN      
        1 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
       61 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
      121 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
      181 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
      241 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
      301 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
      361 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
      421 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
      481 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
      541 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
      601 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
      661 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
      721 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
      781 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
      841 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
      901 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
      961 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
     1021 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
     1081 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
     1141 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
     1201 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
     1261 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
     1321 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
     1381 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
     1441 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
     1501 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
     1561 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
     1621 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
     1681 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
     1741 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
     1801 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
     1861 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
     1921 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
     1981 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
     2041 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
     2101 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
     2161 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
     2221 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
     2281 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
     2341 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
     2401 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
     2461 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
     2521 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
     2581 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
     2641 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
     2701 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
     2761 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
     2821 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
     2881 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
     2941 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
     3001 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgtat cattcacatg
     3061 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
     3121 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
     3181 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
     3241 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
     3301 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
     3361 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
     3421 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
     3481 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
     3541 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
     3601 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
     3661 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
     3721 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
     3781 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
     3841 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
     3901 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
     3961 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
     4021 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
     4081 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
     4141 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
     4201 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
     4261 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
     4321 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
     4381 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
     4441 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
     4501 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
     4561 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
     4621 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
     4681 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
     4741 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
     4801 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
     4861 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
     4921 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
     4981 tccccgctct cccagtaacc acgcagcgtt ggcaggtcat gtgtcgccgc aaccgccatc
     5041 gactgctccg gatacgcttt cggtgcacgg aacgtcttct cgtggtcgtt ttcgaaatag
     5101 agcactttgt aagagtacac accgctgctg cgcagcttac caacaatctc taccggtacg
     5161 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
     5221 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
     5281 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
     5341 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
     5401 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
     5461 ccaaccgatg ctttcaggca atatagttca cggtcacacc aggtttccgc cccaccttcc
     5521 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
     5581 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
     5641 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt cttatacatc
     5701 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
     5761 gcatcaaagg cagcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
     5821 gcggccatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
     5881 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
     5941 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
     6001 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
     6061 gggctggcgc tctccggatt tgccggatag agcgcatgaa tcgggttcag gccaatgaac
     6121 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
     6181 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
     6241 ttattcagca aagcctgcgg ttcgtaacaa cgtttcgggg cgacaatcac ccggcaatgc
     6301 gcgcgctggt cgtcctgggt gagtgttagc gtgtgataac cttccggcag cttcgtcggt
     6361 agattgaacg ctttgccccc cgttacatgg cctttgtact gcgttccttc ttcggtggtc
     6421 agctgccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
     6481 taaaccatga cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
     6541 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
     6601 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
     6661 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
     6721 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
     6781 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
     6841 accagatacg gatcgccgcc ctgtttgccg atactgtgca gcatctggtc gaaggcatgc
     6901 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtc cagcacctta
     6961 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
     7021 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
     7081 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
     7141 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
     7201 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
     7261 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
     7321 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
     7381 tgcaggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
     7441 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
     7501 ataccggtac gaactttcac aaactccgcc agacagactt tattcgcctg cttgatctcg
     7561 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
     7621 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
     7681 cactgtttga tccagcgacg tggggtaata ccgttggtaa cgttatggaa tttgttcggc
     7741 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
     7801 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
     7861 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
     7921 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
     7981 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
     8041 aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc atcggtcagc
     8101 acgcgcagca gttctggaat cgcgatagtt ggatgggtat cgttcagctg aataacttcg
     8161 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
     8221 accgaacagg cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg
     8281 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccttg ctgttcggcg
     8341 cgcaggaaat caccgtcgtt aaatttagtt agatcaaacg gatgcgcatg cgtcgcctgc
     8401 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
     8461 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcatcacttt accgccaatc
     8521 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
     8581 tcatccggcg cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
     8641 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
     8701 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
     8761 tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
     8821 ttcaacaggt tgttgcccgt cagacgacca atcaaaaact ccattgagat gtagttaaca
     8881 tgtcgctgat tcgccaccgg tttggcgaat ggctgagcac gcagcatttc ggccagtgct
     8941 tcactcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
     9001 cgctgccact gacgtgaaag cacttcctga aattgcttat cgttaaaaat aggttgtgac
     9061 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
     9121 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
     9181 gaaatgtgat ctcgaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
     9241 ccatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
     9301 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa gtaaaatatc
     9361 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
     9421 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
     9481 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
     9541 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
     9601 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
     9661 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
     9721 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
     9781 gatcacgagt cctgcgggct acggaaagac caccctcatt tcccagtggg cggcaggcaa
     9841 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
     9901 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
     9961 ggagcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    10021 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    10081 agtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcaccctggt
    10141 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    10201 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    10261 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    10321 cggttgggcg acggcactat agctaatcgc cctctccgcc cggcagaata cccactcagc
    10381 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    10441 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    10501 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    10561 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    10621 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    10681 ggcggcggag ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt
    10741 tcccagcgaa gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat
    10801 tctgcttaat cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc
    10861 gcttaaggcc ctgccgtggg acagtttgct ggaaaatccg cagttagtgt tattgcaggc
    10921 gtggctaatg caaagccaac atcgctatgg cgaagttaac accctgctag cccgtgctga
    10981 acatgaaatc aaggacatca gagaaggcac catgcatgca gaatttaacg ctctgcgcgc
    11041 ccaggtggcg attaacgatg gtaatcagga tgaagcggaa cggctggcaa aactggcact
    11101 ggaagagctg ccgccgggct ggttctatag ccgcattgtg gcaacctcgg tgctgggtga
    11161 agtgctgcac tgcaaaggcg aattgacccg ctcactggcg ctagtgcagc aaaccgaaca
    11221 gatggcacgc cagcacgacg tctggcacta cgccttatgg agtttaattc agcaaagtga
    11281 aattctgttt gcccaagggt tcctgcaaac cgcgtgggaa acgcaggaaa aagcgttcca
    11341 gctgatcaac gagcagcatc tggaacagct gccaatgcat gagtttctgg tgcgcatccg
    11401 agcacaactg ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg cgcgtagcgg
    11461 gattgaagtc ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat
    11521 tcaatgctcg ctggcccgtg gtgatttaga taacgcccgt agccagttga accgtctgga
    11581 aaacctgctg gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg
    11641 ggtgatttac tggcaaatga ccggcgataa agccgccgct gccaactggt tgcgtcatac
    11701 ggctaaaccg gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg
    11761 cgcacaaatc ttgctgggcg agtttgaacc tgcggaaatt gttctcgaag aactcaatga
    11821 aaatgcccgc agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca
    11881 actgtactgg caggccggac gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa
    11941 actggcgaat cgcaccggat ttatcagtca ttttgtcatc gaaggcgaag cgatggcgca
    12001 acaactgcgc cagctgattc aacttaatac gctgccggaa ctggaacagc atcgcgcgca
    12061 gcgtattctg cgagaaatca atcaacatca tcggcataaa ttcgcccatt tcgatgagaa
    12121 tttcgttgaa cgtctgctaa atcatccgga agtgccagaa ctaatccgca ccagcccgtt
    12181 gacccaacgt gaatggcagg tcctggggct gatctactcc ggttacagca atgaacaaat
    12241 tgcgggcgaa ctggaagtgg cggcaaccac catcaagacg catatccgca atctgtacca
    12301 aaaactcggc gtcgcccacc gtcaggctgc cgtgcagcac gcgcagaaac tgctgaagat
    12361 gatgggttat ggggtgtgag tttagccgga taatgcattg gatccggttt acatctttac
    12421 atcattcaat actcacccgc gttacgccat ctgtttctat caaaccaaac cgcaccggca
    12481 agaaacgctc caccaccgcg atattggtca gcagatggca tgaaggatgg gcgaccgtaa
    12541 attctcccgc gcccgccagc gccatcggca gtaccagttg gtcggcgaga tattccccca
    12601 ccgccgctgg gcttgccagg tagcgtttca cctctttcac taactgcgcg gctaccacct
    12661 cggcactgac gcgcttttca ccgacgacga aaaagcgttc agtgatattt tcactttcga
    12721 cttccagcga gacggtatta cccggcccct ggtcgcgcgg caggttatga atattctgtt
    12781 catgcaggga aaaaatcccc gccagtgtag cgatttcacg ctcagcaaca tggcgcggca
    12841 caccagctaa tagaacttct ccacgcatct gcacaatgtt cccgcgctcg ccaagttgca
    12901 aggtgctaaa cgatgccacg ggcgagactt ccgttgccac cacaccgcct ccggcaggat
    12961 aaaaaccgtg acgtaacagc gtggtttgct gatgaattcc tattttcgcc agcagcggct
    13021 ccagcacccg gcggataaaa tccgcaggcg gcgccgacgg gttatcggtg ccgccgctca
    13081 cttcaacacg cgaaggtcca tcggcaaacc acagcgcggg cagcaccgtt tgcagcacca
    13141 gcgtacaact tccggcgcta ccgatagcaa agcggtaatc gccgccgcgc acggtgccag
    13201 gccggaagac cagacgctgc gatcccagct ccgcgccttc caccgttgcc ctacaaattt
    13261 ccgcagccgc ttttaccgcg gtcagatgct ggcgcagcag ccccggtttc gcccgtccgg
    13321 ctcgaatgcc agtgatggta aatggctggc cggttatcat cgacaggctc agcgccgagc
    13381 gcaggatctg cccgccgcct tcgccctgtg cgccatccag cgcaatcatc cttttcatca
    13441 tttatccttt tacgcacacc acctgacgca gggtgtagat aacttccacc agatcgcttt
    13501 gtgccgccat caccgcatca atatctttat acgccatcgg gatttcgtcg atcacttcgg
    13561 catctttacg gcattccaca tgcgcggtgg cacgaatttg gtcttccacg ctgaacagtt
    13621 ttttcgcttt agttcggctc attacccgcc cggcaccgtg gctgcacgaa cagaacgact
    13681 cttcatttcc cagcccacgg acgataaagc tttttgctcc catcgaaccg ggaataattc
    13741 catattgacc agcacgcgca gacaccgcac ctttacgcgt cacgtagatc tcttcaccaa
    13801 agagctgttc tttttgcaca tagttgtggt gacagttgat ctcttccatc gccagcgttt
    13861 gtggctgtct gaccgttttc tgcgtaatgc tctgcaacgc cgttaccaca ttttccatca
    13921 tcgcatcgcg gttaaggctg gcgaaaagct gcgcccaggc cacggctctt aggtaatcat
    13981 caaagtattc cgtaccttcc ataaagtacg ccagatcacg cgacggcaac gtctcaagtt
    14041 ggtcctgcat ctctttttgc gccagatcga taaagtaagt cccgatggcg ttaccaattc
    14101 cgcgtgaacc ggagtgcagc ataatccaca cctgctccga ctcatcaagg cagatttcaa
    14161 taaagtggtt acccgttccc agcgttccca ggtgtttata gttgttagtg ttaaggaaac
    14221 gcggatattt ttgcgttaac cactgataac cggcctccag ctcagcccat ttcgcatcga
    14281 cgttaaaagg cgggttttcc catgcgcctt tatcacgttt acaacgcccg gtagtacgcc
    14341 cgtgtggtac ggccgtttca atcgcttgac gcagctccgc caggttttca ggcaggtctg
    14401 ccgccgttaa cgcggtacgc agcgcgttca ttccacagcc aatatccacg cccaccgccg
    14461 ccggaataat cgcccctttg gtcgggatca cgctaccaat ggtggaacct ttacccaggt
    14521 gtacatcagg cattaccgca atatgtttga aaataaacgg catcttcgcc gtattaataa
    14581 gttgctgacg cgcatcggct tctaccggca cgcctttggt ccacattttt accggggcat
    14641 tttcagtggt cagtaattcg taattcattt tgtttttctc ttttcgttgt ttgcggtcct
    14701 gataattgca accgtcgtgc cagaaaatta aataagcaga ttaatttatt gtttctaaaa
    14761 agattatatc tttatatccg taaacggaga tttcccgcaa agccgattta ccgataatga
    14821 aatatcgcct tttataagga tatctaagat gcgtaaaaca gtggcttttg gctttgtcgg
    14881 taccgtgctg gattatgccg gacgcggcag tcagcgctgg tcaaaatggc gtccgtcact
    14941 ctgtatatac cagcaagaat cgttggtcat cgatcgactg gaattattgc acgatgcccg
    15001 ctcgcgctcg ctatttgaaa cgcttaaacg cgatatcgcc agcgtttcgc cagaaacaga
    15061 agtggtgggc gttgagattg aactgcataa cccgtgggat ttcgaagagg tttacgcctg
    15121 cctgcatgat ttcgcccgtg gttacgagtt tcagccagaa aaagaagact atttaattca
    15181 catcaccacc ggcacccacg tcgcgcagat ttgctggttt ctgctggcgg aagcgcgtta
    15241 tctacccgcc cggttgatac aatcttcaac tccgcgcaaa aaagaacagc cgcgcggccc
    15301 aggtgaagtg acgattatcg atctcgattt aagccgttat aacgccatcg ccagccgctt
    15361 tgccgaggaa cggcagcaaa cgctcgattt cctcaaatcc ggtattgcca cgcgtaaccc
    15421 ccacttcaac cgcatgattg agcagatcga aaaagtggcg attaaatccc gcgcgccgat
    15481 tctgcttaac ggcccgaccg gcgcaggtaa gtcatttctg gcgcgacgca tcttcgagtt
    15541 aaaacaggca cggcatcagt ttagcggcgc gtttgtggaa gtgaactgcg ccaccctgcg
    15601 cggcgatacc gcgatgtcga cgctgtttgg tcatgtgaaa ggcgcgttta ccggggcgcg
    15661 ggaatctcgt gaaggattat tacgcagcgc caacggcgga atgttgtttc ttgatgagat
    15721 tggcgaactg ggcgcagacg aacaggcaat gctgctgaaa gctattgaag agaaaacctt
    15781 ttacccgttt ggcagcgatc gccaggtgag cagtgatttt cagcttatcg ccggaacggt
    15841 gcgcgatttg cgccagctgg ttgccgaagg caaattccgc gaagatctgt acgcgcggat
    15901 caatctctgg accttcaccc tgccgggtct acgccagcgc caggaagata ttgaaccgaa
    15961 cctggattat gaagtggagc gccacgccac actcactggt gacagcgtgc gttttaacac
    16021 cgaagcgcgg cgtgcctggc tggcctttgc aacatctccc caggcaacat ggcgcggtaa
    16081 ctttcgcgaa ctttctgcca gcgtcacgcg gatggccacc tttgccacta gcggacgcat
    16141 cactctggac gtggtggaag atgagataaa ccgtctgcgc tataactggc aggagagccg
    16201 cccctccgcg cttacggcgt tgctgggcgc agaggcagaa aacatcgatc tcttcgaccg
    16261 tatgcaactg gaacacgtta tcgctatctg ccgccaggca aagtcgcttt ctgctgccgg
    16321 acgacagctt tttgatgttt cgcgccaggg caaagccagc gtcaatgacg cggatcggct
    16381 acgcaaatac ctggcgcgtt ttggtctgac gtgggaagcc gtgcaggatc agcacagctc
    16441 cagttgaata tggtggtccg tcagcacctg catcacgctt actggcggcg gggcgtcggt
    16501 gtagacggca tcaaccatgc tgatgctccc catattgacc atcgcattac ggccaaattt
    16561 tgaatggtca accaccagca taacgtggcg cgagttctca ataatggcgc gcttggtacg
    16621 aacttcgtgg taatcgaact ccagcagcga gccgtcgctg tcgatgccgc tgatccccag
    16681 aatgccgaaa tcaaggcgga actgggagat aaaatcgagc gtcgcttcgc caatgatccc
    16741 gccatcgcgg ctacgtaatt cgccaccggc gagaataatg cggaaatctt cttttaccat
    16801 caacgtgtta gcaacgttga gattgttggt gacaatgcgc aaattgctgt gattgagcag
    16861 cgcgtgcgcc accgcttccg gcgtggtgcc gatatcgata aacagcgtcg agccattggg
    16921 gatttgctcc gccactttgc gggcgatgcg ctctttttct tcggtctggg tggctttacg
    16981 atcgtgccac ggcgtgttaa ccgaactgga aggcagcgcc gcgccgccat gatggcgcag
    17041 gatcaggttt tgctctgcca gctcgttgag gtcgcggcga atagtctgcg ggctgacgga
    17101 gaagtgctcc accagttcct cggtactgac ataaccctgc tgtttaacca gttcgataat
    17161 accgttgtga cgttgtgttt gtttcattta taaatccctg gaattatttt cgttttcgcg
    17221 cattgagcga atcaacaaaa gccatcgcta aacccacggc taatccggcg atgtgtgctc
    17281 cgttcgccat cgacatccca aacaaatcaa accatccggc gacaatccag atcagcgcaa
    17341 agataattaa cccacgttgc aggtaaatgc cactttgcgg atcgcgttcg ccacgtagcc
    17401 agacgtagcc catcagcgca tacaccacgc cagaaagccc gccaaaccac ggcccgctga
    17461 atttttgctg catatagccg cttaacaggg cgctgataag cgtaatgaca attagcttac
    17521 cgctaccgag gcgtttttcc accgcgccgc cgagatacca ccaccaaagc aggttaaaga
    17581 ggatatgcat cagcgagaag tgcattaacg cgtgggtgaa gtaacgccag aactcaaatt
    17641 tcagtgttgg atcgaatggc caggccagcc ataacatcac ttcctgatcg ccgagaattt
    17701 gcatggcaat aaacaccacc acgcaggcga tcatcatcac ccaggtcacc ggacctgcgc
    17761 gttcacgcaa ggcagcaaag aaaggataac ggcgataatg caggccactg ccggtatggc
    17821 ctgccagcca gctggccgcc agataacgcg gatctgccgg gttttcgaga aaacgcgcca
    17881 gctccgcccg cacgcgctcg gcctgggact catccgccag ccagacatcg ctttggttat
    17941 gttgttgaat cgtgaggata acaccctgcg tcgccatgta atcaacaaac gcctgcgcca
    18001 cgcgggggtt agcaaaagag gtaatcatca acatcgttgc tgtcgcttat tccacacaaa
    18061 aggggacagt ataaagcgtt acgcgccgta cgccacctct gcgggaaact gtcgctgcca
    18121 ggcttcaaag ccgccgtcaa tgctatagac cacatcgtag ccctgttgca gcagatactg
    18181 cgccgcccct ttgctgctat tgccgtgata acacatcacc atcaccggag tgtcaaagtc
    18241 gttatcacgc ataaacgcgc ccagcgtgtc gttggttaaa tggaaagcct gcaccgcatg
    18301 ccccatagcg aaactctgtg gatcgcgaat atcgaccagc accgcctctt tttcctgcaa
    18361 cttctggtgc gcgtcggcaa cgttaataca ttcgaactga tccatgcgtc tctctttctt
    18421 tacaaacaag tgggcaaatt taccgcacag tttacgtcga agcggcagat aaacgccata
    18481 atgttataca tatcactcta aaatgttttt tcaatgttac ccaaagcgcg attctttgct
    18541 aatatgttcg ataacgaaca tttatgagct ttaacgaaag tgaatgaggg cagcatggaa
    18601 accaaagatc tgattgtgat aggtggcggc atcaatggtg ctggtatcgc ggcagacgcc
    18661 gctggacgcg gtttatccgt gctgatgctg gaggcgcagg atctcgcttg cgcgacctct
    18721 tccgccagtt caaaactcat tcacggtggc ctgcgctacc ttgagcacta tgaattccgc
    18781 ctggtcagcg aggcgctggc tgaacgtgaa gtgctgctga aaatggctcc gcatatcgcc
    18841 ttcccgatgc gttttcgcct gccacatcgt ccgcatctgc gcccggcgtg gatgattcgc
    18901 attggtctgt ttatgtacga tcatctgggt aaacgcacca gcctgccggg atcaactggt
    18961 ttgcgttttg gcgcaaattc agtgttaaaa ccggaaatta agcgcggatt cgaatattct
    19021 gactgttggg tagacgacgc ccgtctggta ctcgccaacg cccagatggt ggtgcgtaaa
    19081 ggcggcgaag tgcttactcg gactcgcgcc acctctgctc gccgcgaaaa cggcctgtgg
    19141 attgtggaag cggaagatat cgataccggc aaaaaatata gctggcaagc gcgcggcctg
    19201 gttaacgcca ccggcccgtg ggtgaaacag ttcttcgacg aggggatgca tctgccttcg
    19261 ccttatggca ttcgcctgat caaaggcagc catattgtgg tgccgcgcgt gcatacccaa
    19321 aagcaggcct acattctgca aaacgaagat aaacgcattg tgttcgtgat cccgtggatg
    19381 gacgagtttt ccatcatcgg caccaccgat gtggaataca aaggtgatcc gaaagcagtg
    19441 aagattgaag agagtgaaat caattacctg ctgaaagtgt ataacgcgca ctttaaaaag
    19501 cagctaagcc gtgacgatat cgtctggacc tactccggtg tgcgtccgct gtgtgatgat
    19561 gagtccgact cgccgcaagc cattacccgt gattacaccc ttgatatcca tgatgaaaac
    19621 ggcaaagcac cgctgctgtc ggtattcggc ggtaagctga ccacctaccg aaaactggca
    19681 gaacatgcgc tggaaaaact gacgccgtat tatcagggta ttggcccggc gtggacgaaa
    19741 gagagcgtgc tgccgggtgg tgccattgaa ggcgaccgcg acgattacgc cgctcgcctg
    19801 cgccgccgtt acccgttcct gactgaatcg ctggcacgtc actacacgcg cacttacggc
    19861 agcaacagcg agctgctgct cggcaatgcg ggaacggtaa gcgatctcgg ggaagatttc
    19921 ggtcatgagt tctacgaagc ggaactgaaa tacctggtgg atcacgaatg ggtccgccgc
    19981 accgacgatg ccctgtggcg tcgcacaaaa caaggcatgt ggctgaatgc ggatcaacaa
    20041 tctcgtgtga gtcagtggct ggtggaatat acgcagcaga agttgtcgct ggcgtcgtaa
    20101 attaacgtaa ggtgatcagg tcagatttca atctggcctg agactgatga caaacacaaa
    20161 actgcctgat gcgctacgct tatcaggcct acgtggttta tgcaatatat tgaatttgca
    20221 tggtcttgta ggccagataa gacgttcacg tcgcatccgg catgaacaaa gcgcactttg
    20281 tcaaaaatct aacctacttt ttaattcagg aaattaccgc aaagcccacg ccatcatacc
    20341 caacgtaaca aaactcaggc acgttcccca ctcgccccga gaaaaatagc attaatgcgc
    20401 ccagcgtcag cataaaaata ttgagcagaa ggtaaattgt tggcgtaaag agtaaattgc
    20461 aaacctcagg aatagcggca agcccagccc ccagcatggc aataaccgct gcaaaaaatt
    20521 gcgtcagtat tgggatgccg aaaaaagtca ttaccagcga ggtcaaaatc cattttctat
    20581 tttgcattat tctttccatt ctttctgaat ggcgaaatta tactccccga gtccccttgc
    20641 cccttctgga cacttttccg aaatgatggc gaaaaaaacg ggaccctttg g
//
LOCUS       DAJTSA010000072        20613 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000072 DAJTSA010000000
VERSION     DAJTSA010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.072"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            96..395
                     /locus_tag="OO369_002755"
     CDS             96..395
                     /locus_tag="OO369_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5982973.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            405..929
                     /gene="ygaP"
                     /locus_tag="OO369_002756"
     CDS             405..929
                     /gene="ygaP"
                     /locus_tag="OO369_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR5982974.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(976..1380)
                     /gene="stpA"
                     /locus_tag="OO369_002757"
     CDS             complement(976..1380)
                     /gene="stpA"
                     /locus_tag="OO369_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR5982975.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2049..2498
                     /gene="alaE"
                     /locus_tag="OO369_002758"
     CDS             2049..2498
                     /gene="alaE"
                     /locus_tag="OO369_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR5982976.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2535..2879)
                     /locus_tag="OO369_002759"
     CDS             complement(2535..2879)
                     /locus_tag="OO369_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR5982977.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3031..3261
                     /locus_tag="OO369_002760"
     CDS             3031..3261
                     /locus_tag="OO369_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5982978.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3608..3853
                     /gene="nrdH"
                     /locus_tag="OO369_002761"
     CDS             3608..3853
                     /gene="nrdH"
                     /locus_tag="OO369_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR5982979.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3850..4260
                     /gene="nrdI"
                     /locus_tag="OO369_002762"
     CDS             3850..4260
                     /gene="nrdI"
                     /locus_tag="OO369_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR5982980.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4233..6377
                     /gene="nrdE"
                     /locus_tag="OO369_002763"
     CDS             4233..6377
                     /gene="nrdE"
                     /locus_tag="OO369_002763"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR5982981.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6387..7346
                     /gene="nrdF"
                     /locus_tag="OO369_002764"
     CDS             6387..7346
                     /gene="nrdF"
                     /locus_tag="OO369_002764"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR5982982.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7701..8903
                     /gene="proV"
                     /locus_tag="OO369_002765"
     CDS             7701..8903
                     /gene="proV"
                     /locus_tag="OO369_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR5982983.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8896..9960
                     /gene="proW"
                     /locus_tag="OO369_002766"
     CDS             8896..9960
                     /gene="proW"
                     /locus_tag="OO369_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR5982984.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10018..11010
                     /gene="proX"
                     /locus_tag="OO369_002767"
     CDS             10018..11010
                     /gene="proX"
                     /locus_tag="OO369_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR5982985.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11202..12381
                     /locus_tag="OO369_002768"
                     /pseudo
     CDS             11202..12381
                     /locus_tag="OO369_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12505..13242
                     /gene="ygaZ"
                     /locus_tag="OO369_002769"
     CDS             12505..13242
                     /gene="ygaZ"
                     /locus_tag="OO369_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR5982986.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13232..13567
                     /gene="ygaH"
                     /locus_tag="OO369_002770"
     CDS             13232..13567
                     /gene="ygaH"
                     /locus_tag="OO369_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR5982987.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13658..14188
                     /gene="emrR"
                     /locus_tag="OO369_002771"
     CDS             13658..14188
                     /gene="emrR"
                     /locus_tag="OO369_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR5982988.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14315..15487
                     /gene="emrA"
                     /locus_tag="OO369_002772"
                     /pseudo
     CDS             14315..15487
                     /gene="emrA"
                     /locus_tag="OO369_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15504..17042
                     /gene="emrB"
                     /locus_tag="OO369_002773"
     CDS             15504..17042
                     /gene="emrB"
                     /locus_tag="OO369_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR5982989.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17106..17621)
                     /gene="luxS"
                     /locus_tag="OO369_002774"
     CDS             complement(17106..17621)
                     /gene="luxS"
                     /locus_tag="OO369_002774"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR5982990.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17771..19327)
                     /gene="gshA"
                     /locus_tag="OO369_002775"
     CDS             complement(17771..19327)
                     /gene="gshA"
                     /locus_tag="OO369_002775"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5982991.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19400..19828)
                     /locus_tag="OO369_002776"
     CDS             complement(19400..19828)
                     /locus_tag="OO369_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5982992.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19825..20391)
                     /gene="yqaB"
                     /locus_tag="OO369_002777"
     CDS             complement(19825..20391)
                     /gene="yqaB"
                     /locus_tag="OO369_002777"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR5982993.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4975 a         5273 c         5148 g         5217 t
ORIGIN      
        1 agcttacgct tttactctca tttacgctgc tgaggctggt atatgttgca tttttagttt
       61 agagtttact taatttagaa aatacttaaa taaatatgac tgaactcgcg caattacagg
      121 ccagtaccga acaggcagcg gccttattga aagcaatgag ccaccctaaa cggttgctga
      181 ttctgtgcat gcttagcggt tcccccggca ccagcgcggg agagctgacg cgcattaccg
      241 gactgagcgc ctctgcgaca tcacagcatc tcgctcgtat gcgggacgaa gggcttatca
      301 acagccaacg ggatgcccaa cgcattctat attccattaa aaatgaggcg gtaaatgcca
      361 ttatcgccac cctgaaaaat gtctattgtc cgtaaggagt cactatggct ttgacaacca
      421 tttcgccgca tgatgcacaa gaattaatcg cacgcggcgc aaagttaatc gatattcgtg
      481 acgctgatga atatcttcgt gaacatattc ctgaagcaga tctggctcca ttatccgtgc
      541 tggaacagtc aggtcttcca cctaaattac gtcgcgagca aattatattc cactgccagg
      601 caggtaaacg caccagtaat aacgccgata aattagcagc gattgccgcc cccgcagaaa
      661 tatttttact cgaagatggg attgatggct ggaaaagagc tggattgcca gtagcggtaa
      721 ataaatctca acccttgccg ttaatgcgcc aggtgcagat cgctgcgggt ggtttaatat
      781 taaacggcgt tgtactgggt tataccgtaa atagcggttt cttcttatta agtggctttg
      841 ttggtgccgg gttactgttt gcaggaatca gcggtttttg tggaatggca aggttgttag
      901 ataagatgcc gtggaaccaa cgagcttgag aagcgacgcc ggacgcgccc tagcagcgac
      961 atccggcctc agtaattaga tcaggaaatc gtcgagagat ttaccttctg ccagcgcctg
     1021 agcaattggc ttcggtgtac ggccctgacc ggtccaggtt ttagtttcac cgttaacatc
     1081 agtgaattta tatttcgccg gacgcggctg gcgtttttta ccagcacgtg gtgcagcagc
     1141 agagctatta cccaataact cttccgggtt aattccgtca gctttcatca gctccagcca
     1201 ggtgctaatt ttttcctgac gctcagccag ttcacgctgc tgctgttctt cttcttcacg
     1261 tctttcttta gtgacaaccc tgaatttttc gagcatttct tcaagaacgt caatggagaa
     1321 ttcgcgagcc atcgcacgga gggtgcgaat gttatttaaa ctttgtaaca ttacggacat
     1381 aataaagaaa accttttaac gccaaaacaa aaaagtattt cgtcgcgtag tgtaagcgat
     1441 cacttattat atttccacaa aatttaatct atttacagtc ctgcgcgaga ttatttcagc
     1501 tgttattttc tgaaactctc agaataattt caagattata tattagccat tttgatgagt
     1561 aatttaggtt attggtgaac agagtggcgc tctctgctcg ctaactccgc aagcctgaca
     1621 aaaccgaagt gaataaaaat gcaatataac agaataaaag aaaacacccc tcactggtgc
     1681 tttctgctgt taatgtacat tttctggcat caaaaatgct gtaaatgacc cggtcatgac
     1741 aaacaccgaa ttctctacgc ttatagcgtt acctcccccc caaactttca ttagtgaaat
     1801 taaaggcaag atcatatcta attgatttaa ttgaaaataa atcatattgg catgtaaaaa
     1861 tccacaatgt acaaaaaacg agccgttacg gaaatatttt atctacaaaa actaactaaa
     1921 taaaaatttt tcactaattg attagtcata gacagcgata tacgctatgc aaaaatgcag
     1981 atggcaatga gatccactgc tttcatctcc attaacatcc cattacgctt ttattaagga
     2041 gcattagcat gttctcaccg cagtcacgct tgcgtcatgc agttgcagat acgttcgcga
     2101 tggttgttta ctgttctgtc gtgaacatgt gtattgaagt tttcctctcc ggaatgagct
     2161 tcgaacagtc tttttattcc agattggtag cgattccggt gaacatctta attgcatggc
     2221 catacggtat gtaccgcgat ctgtttatgc gcgcggcacg caaagttagc ccgtcgggct
     2281 ggataaaaaa tctggctgat atcctggctt atgtgacgtt ccagtcaccg gtgtatgtgg
     2341 cgatcttgtt agtggtgggc gcagactggc atcagattat ggcggcggtc agttcaaaca
     2401 tcgttgtttc gatgttgatg ggggcggttt atggctactt cctcgattat tgccgccgac
     2461 tgtttaaagt cagccgttac cagcaggtaa aagcctgact cttcttttcg cgactggcat
     2521 cgccagtcgc gtctttaact ggcttcgcca aataaaccat tcaaataacg ttcaagcgca
     2581 acacgcgaac taaagccatg cgggataccg taatgctcgt gtcgctcgcc tgctaaatag
     2641 agcggaaatt gctggtaatg atcgcaagtt ttaatatcgg aagcgggttc agccagtgtt
     2701 gaactgcgtt cttttaatgt tggatgaatt accagagcgg tacgtcccat tcgcgcttcg
     2761 cgattaacat agacataatt ttccccacgg cggtaaccat acgctttctg ggttaccaca
     2821 tcgacagtaa aaccgacttt ttcaagtacg cgcgccacct cgtctggtcg taaatacata
     2881 tttgatcctc gttattttca accgcgggcc taccttacct gattgcgcat tcacaacgct
     2941 ttcagaaaag tccataaagc cgcgatgaac ctcagttaag aaaattatgg tctacactga
     3001 aaattacatc gaattctaat gggggatcat atgtttaacc gaccgaaccg caacgacgtt
     3061 gatgatggcg tgcaggatat ccagaatgat gtcaatcaat tagctgacag cctcgaatct
     3121 gtattgaagt cctggggcag cgacgccaaa ggggaagctg aagccgcacg cagtaaagct
     3181 caggcgttgc tgaaagaaac cagggcgcga atgcatggtc gtactcgcgt ccagcaagcc
     3241 gcgcgcgatg ccgttggctg agcggattct tttgttcgtg aaagaccctg gtgtagcgtg
     3301 ggtacagcag ctgcggtagg tatttttatc ggcgcactgt taagcatgcg caaatcgtag
     3361 tgcaaaaatg ataataaata cgcgtctttg accccgaagc ctgtcttcgg ggtttctttt
     3421 tgcctggtga atcacaaaaa tccccctccc ccgtcacgct catatccagg gtaatttcga
     3481 tcactatttg ctatatattg tgtagttgaa tcttttttca actacatcta gtatctctgt
     3541 atcaacagag agacaacccg acgcgtatca tcgcgccgta tcttcatttt aaacggaaat
     3601 acgaatcatg cgcataacta tttatactcg taacgattgc gttcagtgcc acgccaccaa
     3661 acgggcgatg gaaaaccggg gctttgattt tgaaatgatt aatgtcgatc gcgttcctga
     3721 agcagcggaa gcgttgcgtg ctcagggctt tcgtcagttg ccggtagtga ttgctggcga
     3781 tcttagctgg tctggtttcc gtccggacat gattaaccgt ctgcatccag cgccacacgc
     3841 ggccagtgca tgagccagct cgtctacttc tccagcagct ccgaaaacac gcagcgtttt
     3901 atcgaacgtt taggtctgcc cgcggtgcgc atcccgctca atgagcggga acggattcag
     3961 gtagacgagc cttacatcct gatcgtgccc tcttacggcg gcggcggtac ggctggcgcg
     4021 gtgccacgac aggtaattcg ctttttaaac gacgagcaca accgggcgtt gcttcgcggc
     4081 gttattgctt ctggtaatcg caactttggt gaggcgtatg gccgcgccgg agatgtgatt
     4141 gcccggaaat gcggcgtgcc gtggctgtac cgttttgaac tcatgggtac gcaaagcgat
     4201 atcgaaaacg ttcgtaaagg agtaaccgaa ttttggcaac gacaaccgca gaatgcctga
     4261 cgcaggaaac gatggattac cacgcgctga atgcgatgct taatctttac gatagcgcag
     4321 gtcgcattca gttcgataaa gaccgccagg ccgttgacgc ctttattgcg acgcatgtgc
     4381 gtccgaacag tgtgaccttc agtagccagc agcagcgcct gaactggctg gtcaacgaag
     4441 gttactatga tgaaagcgtt cttaatcgct actctcgcga ctttgtcatt acgctgtttg
     4501 cccacgcaca caccagcggt tttcgtttcc agacattcct cggggcatgg aagttttaca
     4561 ccagctatac gttgaagaca ttcgacggta aacgttatct ggaagatttt gccgatcgag
     4621 tcacgatggt ggcgctgacg ctggcacaag gcgatgagac gctggcgttg caactgaccg
     4681 atgaaatgct gtcaggacgc tttcagccag ccacgccaac attcctcaac tgcggtaagc
     4741 agcagcgcgg cgaactggtt tcctgttttt tgctgcgtat tgaagacaat atggagtcga
     4801 ttggtcgggc ggtaaattcc gcactgcagc tgtcgaaacg cggcggcggc gtagcatttt
     4861 tgctgtcgaa tctgcgggaa gcgggcgcgc caattaaacg tattgaaaat caatcttctg
     4921 gcgtaattcc ggtgatgaaa atgttggaag acgcattttc ctatgccaac caactcggcg
     4981 cgcgtcaggg ggcaggtgca gtctatttac atgctcatca tcccgatatt ctgcgttttc
     5041 tcgacacgaa acgggaaaat gccgacgaaa aaatccgcat taaaacactg tcgctgggcg
     5101 tggtgatccc ggatatcact ttccatctgg caaaagagaa tgcgcagatg gcgctgtttt
     5161 cgccctatga cgttgagcga gtttatggca agccgtttgc cgatatcgcc attagcgaac
     5221 actatgacga actggttgcc gatgaacgca tccgcaaaaa atacctcaac gcccgtgatt
     5281 tcttccagcg actggcagaa atccagtttg agtccggcta tccctacatc atgtatgaag
     5341 acacagtaaa ccgtgctaac cctatcgccg ggcgcataaa tatgagtaat ctctgctcag
     5401 aaattttgca ggttaacagc gcctcagagt atgacgagaa tctcgactat gcccgcacag
     5461 gccatgatat ttcctgcaat ttaggttcgt tgaatattgc gcacaccatg gattcccccg
     5521 atttttcccg cacggtagag actgccgtgc gcggtttaac ggcggtgtca gatatgagtc
     5581 atatccgcag cgtgccgtcc atcgaagccg gaaatgccgc ctcgcacgcc atcggactgg
     5641 ggcagatgaa tttacatggt tatctggcga gggaaggcat cgcttatggt tcgccggaag
     5701 ccctggattt caccaatctc tatttctata ccatcacctg gcacgcactg cgtacctcga
     5761 tgttgctggc gcgcgaacgc ggtgaaacct tcgccgggtt caaacagtca cgctatgcca
     5821 gtggtgaata tttcagccaa tatctgcaag gtaactggca gccgaaaacg gcgaaagttg
     5881 gcgaactgtt tgcccgcagc tgtattacgt tacctacccg tgagatgtgg gcgcaactgc
     5941 gcgacgacgt gatgcgctac ggcatataca accagaatct ccaggcggtg ccgccaaccg
     6001 ggtctatctc ttatatcaac catgctacgt cgagtattca tccgattgtg gcgaaagtag
     6061 agatacgcaa agagggcaaa acaggacgcg tttactaccc tgccccgttt atgactaacg
     6121 aaaatctggc gctgtatcag gacgcttacg aaattggcgc agaaaagatc atcgacacct
     6181 acgcggaagc gactcgccat gtcgatcagg ggctgtcgct gacgctgttt ttccccgata
     6241 ccgccaccac tcgcgatatc aacaaagcgc agatttacgc ctggcgcaaa ggtatcaaaa
     6301 cgctctatta catccgcctg cgtcagatgg cgctggaagg cactgaaatt gaaggctgcg
     6361 tctcctgtgc actttaagga atatctatga aactctcacg tatcagcgcc atcaactgga
     6421 acaagatatc tgacgataaa gatctggagg tgtggaatcg cctgaccagc aatttctggc
     6481 taccagaaaa ggtgccgctg tcgaacgata ttcccgcctg gcagacatta actgtcgtag
     6541 aacaacaact gacgatgcgc gtttttactg gcctgacgct gctcgacacg ctgcaaaatg
     6601 ttatcggcgc gccttcgctg atgcccgatg cgctcacgcc tcatgaagag gcggtattat
     6661 cgaatatcag ctttatggaa gcggttcatg cccgctctta cagttcgatt ttctcgacgc
     6721 tgtgccagac caaagatgtc gatgccgcct acgcctggag tgaagaaaac gcaccgttgc
     6781 agcgaaaagc tcagattatt cagcaacatt atcgcggtga tgatccgctg aaaaagaaaa
     6841 tcgccagtgt gtttcttgaa tcttttttgt tctattccgg tttctggctg ccgatgtatt
     6901 tttccagccg cggaaagctg accaataccg cggacctgat ccgtctgatt atccgcgatg
     6961 aagcagtcca cggttactac ataggctata aatatcagaa aaacatggaa aagatatctc
     7021 tgggacaacg tgaagagttg aagagtttcg ccttcgattt gttgctggaa ctctacgaca
     7081 acgagttgca atacaccgat gagctgtacg ccgaaacccc gtgggctgac gatgtgaaag
     7141 cgtttctctg ttacaacacc aataaggctt tgatgaatct gggctacgaa ccgttatttc
     7201 ccgcagaaat ggcggaagtg aatccggcaa tcctcgccgc gctttcgccg aatgccgatg
     7261 aaaatcacga tttcttttcc ggttcaggct cctcttatgt gatggggaaa gcggttgaaa
     7321 cagaagatga agactggaat ttctgagggt gttattttca aaaatatcac tacccgcagc
     7381 agggaaataa ttcccgccaa atagcttttt atcacgcaaa tattctgtag tgatctacac
     7441 tgatactctg ttgcattatt cgcctgaaac tacaatattc aggcgttttt tcgctatcta
     7501 tgacaaaaaa tatcaacttt tctcgatttg ctctcagccc ttatatcacg gaaaattccg
     7561 gcgatttgct cgcatcaata ttcatgccac atttgccctc aggggttgcc tcagattctc
     7621 agtatgttag ggtagaaaaa agtgactatt cccattgggt aatatatcga cataaacaaa
     7681 taaagaaatc attctattgc atggccatta aattaaaaat taaaaatctt tataaaatat
     7741 ttggtgagca tccacagcga gcgttcaaat atatcgaaca aggactttca aaagaacaaa
     7801 ttctggaaaa aactgggcta tcgcttggcg taaaagacgc cagtctggcc attgaagaag
     7861 gcgagatatt tgtcatcatg ggattatccg gctcgggtaa atccacaatg gtacgccttc
     7921 tcaatcgcct gattgaaccc acccgcgggc aagtgctgat tgatggtgtg gatattgcca
     7981 aaatatccga cgccgaactc cgtgaggtgc gcagaaaaaa gattgcgatg gtcttccagt
     8041 cctttgcctt aatgccgcat atgaccgtgc tggacaatac tgcgttcggt atggaattgg
     8101 ccggaattaa tgccgaagaa cgccgggaaa aagcccttga tgcactgcgt caggtcgggc
     8161 tggaaaatta tgcccacagc tacccggatg aactctctgg cgggatgcgt caacgtgtgg
     8221 gattagcccg cgcgttagcg attaatccgg atatattatt aatggacgaa gccttctcgg
     8281 cgctcgatcc attaattcgc accgagatgc aggatgagct ggtaaaatta caggcgaaac
     8341 atcagcgcac cattgtcttt atttcccacg atcttgatga agccatgcgt attggcgacc
     8401 gaattgccat tatgcaaaat ggtgaagtgg tacaggtcgg cacaccggat gaaattctca
     8461 ataatccggc gaatgattat gtccgtacct tcttccgtgg cgttgatatt agtcaggtgt
     8521 tcagtgcgaa agatattgcc cgccggacac cgaatggctt aattcgtaaa actcctggct
     8581 tcggcccacg ttcggcactg aaattattgc aggatgaaga tcgcgaatat ggctacgtta
     8641 tcgaacgcgg taataagttt gtcggcgcag tctccatcga ttcgcttaaa accgcgttaa
     8701 cgcagcagca aggtcttgat gcggcgctga ttgatgcgcc gttagcagtc gatgcacaaa
     8761 cgcctcttag cgagttgctc tctcatgtcg gacaggcacc ctgtgcggtg cccgtggtcg
     8821 acgaggacca acagtatgtc ggcatcattt cgaaaggaat gctgctgcgc gctttagatc
     8881 gtgagggggt aaataatggc tgatcaaaat aatccgtggg ataccacgcc agcggcggac
     8941 agtgccgcgc aatccgcaga cgcctggggt acaccgacga ctgcaccggc tgacggcggt
     9001 ggtgctgact ggctgaccag tacgcctgcg ccaaacgtcg agcattttaa tattctcgat
     9061 ccgttccata aaacgctgat cccgctcgac agttgggtca ctgaagggat cgactgggtt
     9121 gttacccatt tccgtcccgt cttccagggc gtgcgcgttc cggttgatta tatcctcaac
     9181 ggtttccagc aattgctgct gggtatgcca gcgccagtgg cgattatcgt tttcgctctc
     9241 atcacctggc agatttccgg ggtcggaatg ggcgtggcga cgctggtctc gctgattgcc
     9301 atcggcgcaa tcggtgcctg gtcgcaggca atggtgactc tggcgctggt gttaactgcc
     9361 ctgctgttct gtatcgtcat cggtttgccg ttggggatat ggctggcgag aagtccgcga
     9421 gcggcgaaaa ttattcgtcc actgcttgat gccatgcaga ccacgccagc gtttgtttat
     9481 ctggtgccaa tcgtcatgct atttggtatc ggtaacgtgc cgggcgtggt ggtgacgatc
     9541 atctttgcgc tgccgccgat tatccgtctg accattctgg ggattaacca ggttccggcg
     9601 gatctgattg aagcctcgcg ctcattcggt gccagcccgc gccagatgct gttcaaagtt
     9661 cagttaccgc tggcgatgcc gaccattatg gcgggcgtta accagacgct gatgctggcc
     9721 ctttctatgg tggtcattgc ctcgatgatt gccgtcggcg ggctgggtca gatggtactt
     9781 cgcggtatcg gtcgtctgga tatggggctt gccaccgttg gcggcgtcgg gattgtgatc
     9841 ctcgccatta tcctcgatcg cctgacgcag gccgttgggc gcgactcacg cagtcgcggc
     9901 aaccgtcgct ggtacaccac tggccctgtt ggtctgctga cccgcccatt cattaagtaa
     9961 ctctgcactt gcccggtgac gccgggcatt atcaccctgc caaaaaaagg aataacaatg
    10021 cgacatagcg tactttttgc gacagcgttt gccacgctta tctctacaca aacttttgct
    10081 gccgatctgc cgggcaaagg cattactgtt aatccagttc agagcaccat cactgaagaa
    10141 accttccaga cgctgctggt cagtcgtgcg ctggagaaat taggttatac cgtcaacaaa
    10201 cccagcgaag tggattacaa cgttggctac acctcgcttg cttccggcga tgcaaccttc
    10261 accgccgtga actggacgcc actccatgac aacatgtacg aagctgccgg tggcgataag
    10321 aaattttatc gtgaaggggt atttgttaac ggcgcagcac agggttacct gatcgataag
    10381 aaaaccgccg accaatacaa aatcaccaac atcgcacaac tgaaagatcc gaagatcgcc
    10441 aaactgttcg ataccaacgg cgacggtaaa gcggatttaa ccggttgtaa ccctggctgg
    10501 ggctgcgaag gtgcgatcaa ccaccagctt gccgcgtatg aactgaccaa caccgtgacg
    10561 cataatcagg ggaactacgc agcgatgatg gccgacacca tcagtcgcta caaagagggc
    10621 aaaccggtgt tttattacac ctggacgccg tactgggtga gtaatgagct gaagccaggg
    10681 aaagatgtgg tctggttgca ggtgccgttc tccgcactgc cgggcgataa aaatgccgat
    10741 accaaactgc cgaatggcgc gaattacggc ttcccggtca gcaccatgca tatcgttgcc
    10801 aacaaagcct ggaccgagaa aaatccggcg gcagccaaac tgtttgccat tatgcagttg
    10861 ccagtggccg atattaacgc ccagaacgcc attatgcatg acggcaaagc ctcagaaggc
    10921 gatattcagg gccacgttga tggctggatc aaagcccacc agcagcagtt cgatggctgg
    10981 gtgaatgagg cgctggcagc gcagaagtaa tttttattcg ggcggataag gcgttttcgc
    11041 ctcatccgcc gctctgtaca caatgcctga tgcgacgctg acgcgtccta tcatgcctac
    11101 aaaccacatc gcacatttca acaagctatc tttcatccca tattcatcaa catccgctat
    11161 tattgatttc cagcttaatc atcacctgat gaacaaaaat aatgactaaa cctaatcatg
    11221 agcttagccc ggcgctgatc gtgctgatgt ctatcgccac cggtctggcg gtagccagta
    11281 actattacgc ccagccattg ctcgacacca tcgcgcgtaa cttttccctt tccgccagtt
    11341 cggcaggctt tattgttacc gccgcgcagt tgggctatgc cgcaggtcta ctgtttcttg
    11401 ttcccctcgg tgatatgttt gaacgccgcc gcctgattgt ctcgatgacc ttactggcag
    11461 cgggcggtat gttaatcact gccagcagtc agtcactggc gatgatgatc ctcggtacgg
    11521 cattaaccgg tttattctca gtcgtggcac aaattctggt tccgctggca gcgacgctgg
    11581 cttcaccgga taaacgcggc aaagtggttg gcaccattat gagcggtctg ctgttgggga
    11641 tattgctggc acgaaccgtt gccgggttac tggcaaacct cggcggctgg cgcaccgtct
    11701 tttgggttgc ttcggtgtta atggcactga tggcgctggc attatggcgt ggtctgccac
    11761 aaatgaaatc agaaacccac ctcaactacc cacagttgtt gggttccgtt ttcagcatgt
    11821 tcatcagcga taagatcctg cgcacccgcg cgttgctggg ctgcatgacc tttgccaact
    11881 tcagcattct ctggacctca atggcctttt tgcttgccgc tccacctttt aactacagcg
    11941 atggtgacat tggtctgttt gggcttgcgg gagctgccgg agcgttgggc gctcgtccgg
    12001 cgggcggttt tgccgacaag ggcaaatcgc accacaactt tcggtctgct tctgctgtta
    12061 ctttcatggc tggcaatctg gtttggtcac acttccgtac tggcgttgat tatcggcatt
    12121 ctggtgctgg acctcaccgt acagggcgtg catatcacta accagacggt aatttatcga
    12181 atacatcctg atgcgcgtaa tcgcctgacc gcaggttaca tgaccagcta ctttattggc
    12241 ggtgccgccg gttcgctaat ttcagcctca gcctggcaac atggcggttg ggctggcgtt
    12301 tgtctggctg gcgcgacgat tgccctggtt aacttactgg tctggtggcg aggttttcat
    12361 cgtcaggaag ccgcaaatta agcaatcagc aaccgttttc gtggctttac acttataagg
    12421 gtgttaagaa gcccatcagt ctgataaggt taagatattc attcagtcta tttataatat
    12481 taacaatcgt taagcgtaca ctctatggaa agccctactc cacagcctgc tcctggttcg
    12541 gcgaccttca tggaaggatg caaagacagt ttaccgattg ttattagtta tattccggtg
    12601 gcctttgcgt tcggtctgaa tgcgacccgt ctgggattct ctcctctcga aagcgttttt
    12661 ttctcctgca tcatttatgc aggcgcgagc cagttcgtca ttaccgcgat gctggcagcc
    12721 gggagtagtt tgtgggttgc tgcactgagc gtcatggcaa tggatgttcg ccatgttttg
    12781 tatggcccgt cactgcgtag ccgtattatt cagcgtctgc aaaaatcgaa aaccgcccta
    12841 tgggcgtttg gcctgacgga tgaggttttt gccgccgcca ccgcaaaact ggtacgcaat
    12901 aatcgccgct ggagcgagaa ctggatgatc ggcattgcct tcagttcatg gtcatcgtgg
    12961 gtatttggta cggtaatagg ggcattctcc ggcagcggct tgctgcaagg ttatcccgcc
    13021 gttgaagctg cattaggttt tatgcttccg gcactcttta tgagtttcct gctcgcctct
    13081 ttccagcgca aacaatctct ttgcgttacc gcagctttag ttggtgccct tgcaggcgta
    13141 acgctatttt ctattcccgt cgccattctg gcaggcattg tctgtggctg cctcactgcg
    13201 ttaatccagg cattctggca aggagcgccc gatgagctat gaggttctgc tgcttgggtt
    13261 actggttggc gcggcgaatt attgcttccg ctatttgccg ctgcgcctgc gtgtgggtaa
    13321 tgcccgccca accaaacgtg gcgcggtagg tattttgctc gacaccattg gcatcgcctc
    13381 gatatgcgct ctgctggttg tctctaccgc accagaagtg atgcacgata cacgccgttt
    13441 cgtgcccacg ctggtcggct tcgcggtact gggtgccagt ttctataaaa cacgcagcat
    13501 tatcatccca acactgctta gtgcgctggc ctatgggctc gcctggaaag tgatggcgat
    13561 tatataaccc acaagaatca tttttctaaa acaatacatt tactttattt gtcactgtcg
    13621 ttactatatc ggctgaaatt aatgaggtca tacccaaatg gatagttcgt ttacgcccat
    13681 tgaacaaatg ctaaaatttc gcgccagccg ccacgaagat tttccttatc aggagatcct
    13741 tctgactcgt ctttgcatgc acatgcaaag caagctgctg gagaaccgca ataaaatgct
    13801 gaaggctcag gggattaacg agacgttgtt tatggcgttg attacgctgg agtctcagga
    13861 aaaccacagt attcagcctt ctgaattaag ttgtgctctt ggatcatccc gtaccaacgc
    13921 gacgcgtatt gccgatgaac tggaaaaacg cggttggatc gaacgtcgta aaagcgataa
    13981 cgatcgccgc tgcctgcatc tgcaattaac ggaaaaaggt cacgagtttt tgcgcgaggt
    14041 tttaccaccg cagcataact gcctgcatca actctggtcc gcgctcagca caacagaaaa
    14101 agatcagctc gagcaaatca cccgcaaatt gctctcccgt ctcgaccaga tggaacaaga
    14161 cggtgtggtt ctcgaagcga tgagctaacg cgtcatctcg ctcaaaaatc cagatttata
    14221 aaagaaaaaa tgaccggcca gcatcgcaac atgctggcct ttttggcaag caggtcggct
    14281 cagccgatga gttaagaaga tcgtggagaa caatatgagc gcaaatgcgg agactcaaac
    14341 cccgcagcaa ccggtaaaga agagcggcaa acgtaagcgt ctgctcctcc ttctcacctt
    14401 gctctttata attattgccg tagcgatagg gatttattgg tttttggtac tgcgtcattt
    14461 cgaagaaacc gatgacgcat acgtggcagg gaatcagatt caaattatgt ctcaggtgtc
    14521 tggcagcgtg acgaaagtct gggccgataa caccgatttt gtaaaagaag gcgatgttct
    14581 ggtcactctc gacccgacag atgctcgcca ggcgtttgaa aaagccaaaa ctgcactggc
    14641 ttccagcgtt cgccaaaccc accagctgat gattaacagc aaacagttgc aggcgaatat
    14701 tgaggtgcaa aaaatcgccc tcgcgaaagc acaaagcgac tacaaccgcc gtgtgccgct
    14761 gggcaatgcc aacctgattg gtcgcgaaga gctgcaacac gcccgcgacg ccgtcaccag
    14821 tgcccaggcg caactggacg tcgctattca acaatacaat gccaatcagg cgatgattct
    14881 ggggactaaa ctggaagatc agcctgccgt gcaacaggct gccaccgaag tacgtaacgc
    14941 ctagctggcg ctggagcgta cccgtattgt cagtccgatg accggttatg tttcccgtcg
    15001 tgccgtgcaa ccgggtgcgc aaattagccc aacgacgccg ctgatggcgg tcgttccagc
    15061 caccaatatg tgggtggatg ccaactttaa agagacgcag attgccaata tgcgcatcgg
    15121 tcagccggtc accatcacca ccgatattta cggcgatgat gtgaaataca ccggtaaagt
    15181 ggttggtctg gatatgggca caggtagcgc gttctcactg cttccggcgc aaaatgcgac
    15241 cggtaactgg atcaaagtcg ttcagcgtct acctgtgcgt atcgaactgg accagaaaca
    15301 gctggagcaa tacccgctgc gcattggttt gtccacgctg gtgagcgtca ataccactaa
    15361 ccgtgacggt caggtactgg caaataaagt acgttcaaca ccggtcgcgg taagtaccgc
    15421 gcgtgaaatc agcctggcac ctgtcaataa actgatcgac gatatcgtaa aagctaacgc
    15481 tggctaatcc agaggtgcgt gtgatgcaac agcaaaaacc gctggaaggc gcgcaactgg
    15541 tcattatgac gattgcgctg tcactggcga cattcatgca ggtgctgaac tccaccattg
    15601 ctaacgtggc gatccccact atcgccggga atctgggctc atcgctcagc caaggaacgt
    15661 gggtaatcac ttctttcggg gtggcgaatg ccatctcgat cccgcttacc ggctggctgg
    15721 caaagcgcgt cggggaagtg aaactgttcc tttggtccac catcgccttt gctattgcgt
    15781 cttgggcgtg tggtgtctcc agcagcctga atatgctgat cttcttccgc gtgattcagg
    15841 ggattgtcgc cgggccgttg atcccgcttt cgcaaagtct attgctgaat aactacccgc
    15901 cagccaaacg ctcgatcgcg ctggcgttgt ggtcgatgac ggtgattgtc gcgccaattt
    15961 gcggcccgat cctcggcggt tatatcagcg ataattacca ctggggctgg atattcttca
    16021 tcaacgtgcc gattggcgtg gcggtggtgt tgatgacact gcaaaccctg cgcggacgtg
    16081 aaacccgcac cgaacggcgg cggattgatg ccgtggggct ggcactgctg gttattggta
    16141 tcggcagcct gcagattatg ctcgaccgcg gtaaagagct ggactgtttt tcatcacagg
    16201 aaattatcat tcttaccgtg gtggcggtgg tggctatctg cttcctgatt gtctgggagc
    16261 tgaccgacga taacccgata gtcgatctgt cgttgtttaa gtcgcgcaac ttcaccatcg
    16321 gctgcttgtg tatcagcctc gcgtatatgc tctacttcgg cgctattgtt ctgctgccgc
    16381 agttgttgca ggaggtctac ggttacacgg cgacctgggc aggtttggcc tctgcgccgg
    16441 tagggattat tccggtgatt ctgtcgccga ttatcggccg cttcgcgcat aaactggata
    16501 tgcggcggct ggtaaccttc agctttatta tgtatgccgt ctgcttctac tggcgtgcct
    16561 atacctttga accaggtatg gatttttgcg cgtcggcctg gccgcagttt atccaggggt
    16621 ttgcggtggt ctgcttcttt atgccgctga ccaccattac gctgtctggt ttgccaccgg
    16681 aacgactggc ggcggcatcg agcctctcta actttacgcg aacgctggcg gggtctatcg
    16741 gcacgtcgat aaccacgacc atgtggacca accgcgagtc gatgcaccat gcgcagttga
    16801 ctgagtcggt aaacccgttc aacccgaatg cccaggcgat gtacagtcaa ctggaagggc
    16861 ttgggatgac gcaacagcag gcatcaggct ggattgccca gcagatcacc aatcaggggc
    16921 tgattatttc cgccaatgag atcttctgga tgtcagccgg gatattcctc gtactgctgg
    16981 ggctggtgtg gtttgctaaa ccgccatttg gcgcaggtgg cggcggaggc ggtgcgcact
    17041 aagtacaact aagccagttc atttgaactg gcttttttca attaattgtg aagatagttt
    17101 actgactaga tgtgcagttc ctgcaacttc tctttcggca gtgccagttc ttcgttgctg
    17161 ttgatgcgta cctcacgttc cagaatgcta cgcgcaatat cctgcgcttc ctgcaacgag
    17221 tgcatctggt aagtgccaca ctggtagacg ttcagttccg ggatctgatt ctgatcctgc
    17281 actttcagca cgtcttccat tgccgctttc caggcatcag caacacgctg ctcatctggc
    17341 gtaccaatca gactcatata aaaaccggtg cggcagccca ttggcgagat atcgataatc
    17401 tctacaccat taccgttaag atggttacgc ataaaaccag caaacaggtg ctccagggta
    17461 tggatccctc tttctggcat cacttctttg ttcggcacgc agaagcgcag atcgaacacg
    17521 gtgattgcgt cgccatgcgg ggtgttcatt gttttcgcca cccgaactgc aggcgcttcc
    17581 atccgggtat gatcgactgt gaagctatct aacaacggca tttagccacc tccggtaatt
    17641 tttttaaaaa ttttctgaac tctttcttcc caggcgagtc tgagtatatg aaagacgcgc
    17701 atttgttatc atcatccctg aattcagaga tgaaattttg gccactcccg agtggccttt
    17761 ttcttttctg tcaggcgtgt ttttccagcc acaccgcaaa cggttcggta tcagcggttt
    17821 ccatttcctg ctgacggcgt tcagatgcct cgcgctcggc tacaaaatcc tcttcgcgca
    17881 gaatttccag cggctcttca cgcagcagat tacggtaggc ttctgcaaac gctttgcctg
    17941 ttccgccaat accagtatca atcatagacc ttaagatacg ggcagagaaa gtcagatcgg
    18001 gattatcgaa gcaggcaacc agttcatcac acactttctg atacgcttcg ccgccgttaa
    18061 tgctatccag cgtttgcgcg acgcgtttca gatcgcggaa cagatcttta cccacctgca
    18121 gtaacgggaa ctgcgcggtt tcgcagccga tacccagcgt caggcccggt ttgcgacctt
    18181 caaggatcac ccggttccag ttaacgcgcg tacaggcaag ttcgctactg ctcatttccg
    18241 gtgcatcagc cagcgcacac cagaccataa acaggtcgag gaatcgcacc tgctgttcat
    18301 ctacaccaat cggcgagaac gggttgatgt ccagcgaacg cacttcaata tattcaatgc
    18361 cgccacgtaa cagcgcatca gaaggtgact cgccgctgcg ggtaacgcgt tttggacgaa
    18421 tcggcgcata cagttcgttt tcaatctgca gcacgttgct attgatttgc agcctcttac
    18481 cgtctttctc aataccaatc ttcgcgtact cttccgatgg cgttttgatt gcctgtttaa
    18541 ggcccgctac gtactcgtaa agatcgttga aggtaatacc aagattgctt tgcgatttat
    18601 tggtatagcc gagatcgctc aaacgaagag aggtcgcata cggcaggtaa tacataccgc
    18661 actcggtttt ctcaaacggc agcgacgttg gttttccttg caggaaagaa gaacaaatcg
    18721 ccggagatgc accaaacaga taaggaataa cccaaccgaa acgatagtaa ttgcggataa
    18781 cgcggaaata gcccgcagaa attttctctt tggcatcagc gcccgagata tcaccgcact
    18841 tcgcttgcca gaatgccatt ggcaaagaga aattgtagtg cacgccggaa atggtttgca
    18901 tcagcgcgcc gtagcgattt ttcagccctt cacgatacag cgttttaaag cgtccggtgt
    18961 tagaagtgcc gtactgtgcc agttcgatgt cctgaccttc tgcgatgtag catggcatac
    19021 ttaacggcca catccgctca tcgcccatat tgcgcgccgt ataacgatgc agatcgcgca
    19081 taaaggtcag catatgttca atatcaccat ccactggtgt aatgaattcc agcaatgctt
    19141 ccgcaaaatc ggtagtaatc catttgtgcg tcagtgcgga acctaatgct tcaggatgac
    19201 ctgttgttgc cagtgtgcca tcagcattaa cacgcaaagt ttcgcgctcc agcccacgct
    19261 gtatcccctt taacgcctga ggatgttttt ccagccaggc cagcgcctgt gatacgtccg
    19321 ggatcaaatt gacctcccgc ctgtcaaaat cgttttaatt agcataactg taatggtgac
    19381 catatgtgca ggcctacaat tagtgccacc acatcatgcc ctgaacggtc gctgctgcaa
    19441 ctgcaacata gcgtaacgct ttaccaaggc acaaaaaaaa gattactggc ccccaggaga
    19501 tgcgcatcca tcccgctaac agacacagta aatcgccaac cacgggcatc cagcttaata
    19561 atagcgtgac tgcaccatag cgtttcagcc agccggtagc tttctcttgc cagcgcgatg
    19621 ttttacgcaa tggaaagaaa cgcccaagga taacgttagt taaccctcca aggctattac
    19681 ccattgttgc tgttaaaact aaaacccagg gatgactgat cccggaaagc aacattgcca
    19741 ccagcacgac ttcggagttg ccgggtaata gtgtagcgct gagaaaacta ctggcaaaca
    19801 acgagaaaag cgataacgct tcactcacag caagcgaaca tccacagcgt ccatgcctgc
    19861 tgcacgggcc gcctgaatac cgaaatcggc atcttcaaag accacacact gcgtcggttg
    19921 cacgcccata cgctgcgcgc acaacaaaaa tgtgtctggc gcgggtttat ggtgtttgac
    19981 gtgatcagca gcgacgacgg cgtcaaaata acggcgtaat cccaggtgcg ccagcaatgc
    20041 ctcagcgatg gcgctttcac tccccgttcc tacagccatt gggcgacgac catgccaact
    20101 tttcaccact tcaacaagag gaagcggctc gacactatcc agcagcatac ttcttactgc
    20161 ttctgttttt tcacgcgcta acgcatgcgg gtcgagatcg gcctgattca gctcaataat
    20221 tgcctgagca atacgccagg tgggcgatcc attaagcgca atcatcgcct gaatatcgta
    20281 ctgaagaccg tagtgcccta atacttcgcg ccacgcttta cggtgcgtag gcgccgtatc
    20341 caggattgtg ccatccatat caaaaattaa acctgcataa cgctcgtaca tggtcttctc
    20401 gcaagtcgaa aaatatgacg ctactttagc gtaattgctt gattttgtcg ctgatggcga
    20461 aggagaagaa aattcaggaa atggataaag tagttattta gaaatgagat atttttgaag
    20521 gaaatttttt ggagaatatg gtgcatccgg gaggattcga acctccgacc gctcggttcg
    20581 tagccgagta ctctatccag ctgagctacg gat
//
LOCUS       DAJTSA010000073        20521 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000073 DAJTSA010000000
VERSION     DAJTSA010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.073"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="OO369_002778"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="OO369_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR5982994.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="OO369_002779"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="OO369_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCR5982995.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="OO369_002780"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="OO369_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR5982996.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OO369_002781"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OO369_002781"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR5982997.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OO369_002782"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OO369_002782"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR5982998.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESYIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="OO369_002783"
     CDS             4449..4814
                     /locus_tag="OO369_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR5982999.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4964..5482
                     /locus_tag="OO369_002784"
     CDS             4964..5482
                     /locus_tag="OO369_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR5983000.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="OO369_002785"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="OO369_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="OO369_002786"
     CDS             6713..7195
                     /locus_tag="OO369_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR5983001.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="OO369_002787"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="OO369_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR5983002.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="OO369_002788"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="OO369_002788"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR5983003.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="OO369_002789"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="OO369_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR5983004.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="OO369_002790"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="OO369_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR5983005.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="OO369_002791"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="OO369_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR5983006.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10939..11730
                     /locus_tag="OO369_002792"
     CDS             10939..11730
                     /locus_tag="OO369_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR5983007.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="OO369_002793"
     CDS             11751..13037
                     /locus_tag="OO369_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5983008.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMPLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="OO369_002794"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="OO369_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR5983009.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="OO369_002795"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="OO369_002795"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR5983010.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="OO369_002796"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="OO369_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR5983011.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="OO369_002797"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="OO369_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR5983012.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="OO369_002798"
     CDS             complement(16985..17275)
                     /locus_tag="OO369_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR5983013.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="OO369_002799"
     CDS             complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="OO369_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR5983014.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="OO369_002800"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="OO369_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR5983015.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="OO369_002801"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="OO369_002801"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="OO369_002802"
     CDS             19137..20378
                     /locus_tag="OO369_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5983016.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5300 a         5358 c         4815 g         5048 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggctaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt caccttgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatataact ttcgatcatc agaccaataa ttgagcgatt
     3361 accatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatttgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgctcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acatcccctt taaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttactta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaagggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gcctcatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attgcaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgccttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcaacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcca
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctgatgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccaa gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgcccctca accgctatcg tcttcgacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtaccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgttgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg caagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttaa ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtattgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacctggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtatttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgctccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagacacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgatccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgcctgtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggatcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg cgggtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattctgca ctccagaagt
    20521 t
//
LOCUS       DAJTSA010000074        20287 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000074 DAJTSA010000000
VERSION     DAJTSA010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20287
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.074"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(61..819)
                     /gene="aroD"
                     /locus_tag="OO369_002803"
     CDS             complement(61..819)
                     /gene="aroD"
                     /locus_tag="OO369_002803"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR5983017.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="OO369_002804"
     CDS             complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="OO369_002804"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR5983018.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1728..2999)
                     /locus_tag="OO369_002805"
     CDS             complement(1728..2999)
                     /locus_tag="OO369_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5983019.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLSEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3182..4387)
                     /locus_tag="OO369_002806"
     CDS             complement(3182..4387)
                     /locus_tag="OO369_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5983020.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4487..4843)
                     /locus_tag="OO369_002807"
     CDS             complement(4487..4843)
                     /locus_tag="OO369_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCR5983021.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4979..5086)
                     /gene="rprA"
                     /locus_tag="OO369_002808"
     ncRNA           complement(4979..5086)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO369_002808"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="OO369_002809"
     CDS             complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="OO369_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR5983022.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6773..9829
                     /locus_tag="OO369_002810"
     CDS             6773..9829
                     /locus_tag="OO369_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5983023.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9826..10236
                     /gene="menI"
                     /locus_tag="OO369_002811"
     CDS             9826..10236
                     /gene="menI"
                     /locus_tag="OO369_002811"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR5983024.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10336..10524
                     /locus_tag="OO369_002812"
     CDS             10336..10524
                     /locus_tag="OO369_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR5983025.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11072..11440
                     /gene="sufA"
                     /locus_tag="OO369_002813"
     CDS             11072..11440
                     /gene="sufA"
                     /locus_tag="OO369_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR5983026.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11449..12936
                     /gene="sufB"
                     /locus_tag="OO369_002814"
     CDS             11449..12936
                     /gene="sufB"
                     /locus_tag="OO369_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR5983027.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12946..13692
                     /gene="sufC"
                     /locus_tag="OO369_002815"
     CDS             12946..13692
                     /gene="sufC"
                     /locus_tag="OO369_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR5983028.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13667..14938
                     /gene="sufD"
                     /locus_tag="OO369_002816"
     CDS             13667..14938
                     /gene="sufD"
                     /locus_tag="OO369_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR5983029.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14935..16155
                     /gene="sufS"
                     /locus_tag="OO369_002817"
     CDS             14935..16155
                     /gene="sufS"
                     /locus_tag="OO369_002817"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR5983030.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16168..16584
                     /gene="sufE"
                     /locus_tag="OO369_002818"
     CDS             16168..16584
                     /gene="sufE"
                     /locus_tag="OO369_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR5983031.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16733..17737
                     /gene="ldtE"
                     /locus_tag="OO369_002819"
     CDS             16733..17737
                     /gene="ldtE"
                     /locus_tag="OO369_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR5983032.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17800..18036)
                     /gene="lpp"
                     /locus_tag="OO369_002820"
     CDS             complement(17800..18036)
                     /gene="lpp"
                     /locus_tag="OO369_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR5983033.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18347..19759)
                     /gene="pykF"
                     /locus_tag="OO369_002821"
     CDS             complement(18347..19759)
                     /gene="pykF"
                     /locus_tag="OO369_002821"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR5983034.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5317 a         4861 c         5176 g         4933 t
ORIGIN      
        1 cggttaaaaa tacttttttt atttcggcgg gggagggtat ttccaccgaa atattattgc
       61 ttatgcctga tgtaaaatag ttaataccgt gcgcaaatca tttaccgaga tttgccctgg
      121 cgcagaggct ttttttaccg caccaaaagt tgccgccgag ccaaatactt caccaaccag
      181 acgagaaatt acgccagttt ttgccatcga catcgtgatg attggacgat cggcatactg
      241 ctcctgcatc tccagggtcg cggcaagcaa cgtcagcaca tcgctggtac tttgcggcat
      301 cagcgcaatc ttaggaatat cggcgtcgaa ggactgcatt ttgcgcagac gggcaatgat
      361 ttcttcggct tccggcgttt tatggaagtc atggttggac atgacaactt tcacatcatg
      421 cgcgtgggcg taggcgacgg tttctttgac ctgatcatcg ccggtaaata actccagatc
      481 gatcatatca accaggccgc tgtcgatggc tgcacgattg agagcaatat aagcctcggt
      541 ggaaatcgcc tgctcgccgc cttctttggc actgcggaag gtaaacagca gcggtttttc
      601 tggcatggtt tcacggagaa tttttgccgc cgccatgaca gactccacat tggagaggtc
      661 ggcaaagtgg tccacacgcc attccagaat atcaaagtcc gcttcacgat aggcgagagc
      721 ttcggatttc acgcgggcga tatctttcgc catcagcgag acgatgattt taggtgcgcc
      781 cgcaccaatg acgagatctt ttacagttac ggttttcatt ttttaccctt tctgcacgcg
      841 gtcagcctgt caggcaccga accccatgac ctgtttaaca tattccagag ggaaatcttt
      901 gccagtccat aacgtgaact gttcagcccc ttgccacaac aacatgccgt atccatcaat
      961 cgttttgcaa ccagcttgtt gtgcctgctg caataacttc gtcatatgcg ggttatacac
     1021 gcattcagtg accagcagtc ccggatgtaa cagactgata tcattaacca atgattcatt
     1081 ctcaaggggt ttcataccta cttttgtgcc attggttaaa atgtcggcgg aagccagggc
     1141 ttcagcaaag gcttgctgat cggcgagatc ggtatccgtg acgacacaat cggtgttttc
     1201 gttaacccgc tgcgcgaagg cgagggcttt atcgaagaac tcatcccgac ggttaaagag
     1261 tttaatttct tttaaacctt caattgcccc ctgcgcgcca atcgccgttg aggcacctcc
     1321 ggcccctaac agcaccatcg ttttgccttt gatatcaaaa ccgctctctt taatggcgcg
     1381 aatatggccc gtgccgtcgg tgttatagcc acgcagatag ccatcatcat taacgatggt
     1441 gttgatggca cccaccagtt tggctgctgg cgttaattca tcaacatatt cacacgccag
     1501 ttgtttgttc ggcatcgaca caccagttcc gcgcattttg agggctttta atccttcaat
     1561 tgctccggga aagctatcgt tatccacttc gaaggccata taggtaaatg gcaatcccgc
     1621 tttttctaag gctttattct gcatttcagg cgataaactg tggcggatag gataagccat
     1681 caatccaatc aattcgtatt ttgcggtaac atccataatt aactccttta acctctatgc
     1741 ttaattgtgt ttaaccgggt gctaaatttg cgttcgccaa accgcacatc attctcagga
     1801 ataataaaaa cgcggtaata gcggctaaaa acaataattg cggtaatcag ggccagcagc
     1861 gcgaaagtaa aatcgagaac aatgatatat tgcaggccga tgttcgacag ataaccggta
     1921 atcagcggaa taacaaagtt agccagtcca cccatcatca tataaatact ggtgactttg
     1981 gctttgcttt tggggaaaaa ctctgacatg accgaaacac cgagctgtaa aatgccgcca
     2041 gctgctgaga aaccgataac aaaggcgccg gcattacaca ccagtggaga agggtacagg
     2101 tagataatgg cagctgttat tgttgccagc gcagagttaa atacattagc ccagatgggc
     2161 cggaccattt ttttcagtag tgcggcaaaa ataaagacac agaccaacga gcccatactg
     2221 taataagaga tggtttttaa tgcctcagct tctgacatac cagcaaaagc catcgcatat
     2281 ttgggcatcc acaccacaat cacataaaag gtcgagaatg cggctacacc gaacagtacc
     2341 gatgaaacac cttccagcca gactaaaggt ttgctgttca tttgcggtaa ttcattagct
     2401 acgctggcgt ccaccaactg gctggggaat ttgcttttca acagcatcag cgtgatcagt
     2461 acaaacagaa tacccggaat aatcagccca tagccgtacc agatattatt gagcaacata
     2521 tagctcacca gcattgggta gaacatttgc ccaaatgaca ccatcgcttt aaccagtatg
     2581 accgccgaac cagaggcttt cggaaagcat tccatgagcg cggggtagcc acccgtatcc
     2641 agcgctgagt tagcgatacc tacgcacact gccagaccgt aggcgagagt taaattcggg
     2701 caagcgggaa taccaaagaa gaatagcaga tacattatta ctgccattaa tatcaccgcc
     2761 cgacgaccaa acttatcgga gatcacaccg aagaataaaa tactgatcaa tcgccccaaa
     2821 ccgataccgg aaattaaata ggcaatgccc gcgttgtcag tggaaaactt ttctgacaga
     2881 gatgtcatat tttgggcaag cgtaataaca ctaatgccgt gcaggaaata gctgaagtaa
     2941 atacaaagaa cagccaggat aaatggcgtg ctgaaagata atttcgttac cgtacgtatt
     3001 gttctccgac ggtttacata attactatac tgaagatgat tttccctggg tacatggcat
     3061 aaagtttatg tttccaggaa tattgcatgt catagtttgt ttttgatcgc gattttcctt
     3121 agttagcgca gaaagagtta tttatccgta agatcttgca tgacctgaaa agaatattgc
     3181 attacccacc cggagcgaca ttttccttta gcgagtgatg ccgcgttttt ttgcggctgc
     3241 gtaagccgat aaacagtgcc agtaaaaaac cgatggcagt atcgaaccac ataatatcgg
     3301 caatacttct ttgggacaga tgagccgtaa tcaacggaat agtaaaggtc gcaatactgc
     3361 ccgtactgta atagatccct gtagctttac ctttagcgta agggaaacgt tcagccatta
     3421 acgtcaggcc aatttgcaca acacctccag cagaggtaaa accaatgaca aaagcaaata
     3481 ttatcaccac ataaaatgtg ggatgcaggc agacggtaaa cagagcaata aatgagataa
     3541 aggtgtacag catcagtaat gttgttgggc gaacggtatt acgaatgagt ggagcggtaa
     3601 taaatacaca aagcagcgaa cccacggtgt agatactgag tagtttgatc gacatagtgt
     3661 atgacatgcc tgcaacaaat tgtccgtact gtgccagcca ctggctaacc agataaaacg
     3721 ttgccattga gatatagcca tataaggtat aactggctaa atcgataatt gaacagcgat
     3781 gttccgtaga gctggtggtt ttctttatga caggtaagcg acgacccgga tggggtggga
     3841 acgtacaacg gtataaaaac agagcgttaa taaacataat gcctgcagca atcataaagg
     3901 accaaccgaa ccacagttca gcccacacta acaggctaat gattagcggt aataaaaatt
     3961 gtccgctgga aacaaatgct ttaattaaaa tattggctgt cccaggtgag cgtggaaaag
     4021 cttccatcaa actgggataa gtgcctgcat cgagaaaact gtttgccatt cccgccagaa
     4081 agccaaaaac ataagcgata atgatgttat tggtctgcag gatgtcaaaa aagaaggcca
     4141 tatagcagca catcccgagc atgataaaag ggcggcgacc aaagcgatcg gataataatc
     4201 ctgcaaaaag caagacactt aatcgaccaa tgcccagcga tgagataact atcgagacac
     4261 ccgcggcatt agtctgccaa agtgtctcca gcgaggccat attcaggctc atcaaaatga
     4321 cgcccatacc atgcaccagg taattaaaat acaacccaag tgcggtaggg taatagggat
     4381 ttttcatagg ttctacttag caaaggtgct ttaaaaagtg tagcgatgtt gtgtcagtat
     4441 ccattcatta ttgttgtaac aacgacaatt cctttctgtg gaaaaattaa caaagccgct
     4501 ccagatcgtg tgcatataat tcagcggcta ccgattgata gatcttccag tcaataaaat
     4561 tgccatcagg gtagacttgt tgaaacgcgg ttaagtcggg gaaaaaacgc atggtgttgt
     4621 tgccgatgcg gttatttatt ttctctatga tggcatgaag atgttttttt cgttgctgac
     4681 gcatagccaa cagttgctcg actacacaat caggaatggc gcatttgcca ttttcccact
     4741 gacgccagct ttcactgtca ccggtctggg caatccaggt cgcgcattca tcaatagtca
     4801 tggcaaaaat atggcggagt gcctggagtt cataagcgtt catcgtgtaa tggctgcatg
     4861 aaataaacaa taaaatataa ataacattat cagcctgctg acggcttgaa gagagtcaca
     4921 gtatcttgtg caacattatt ggaaagagtg aggggcgagg tagcgaagcg gaaaaatgtt
     4981 aaaaaaaagc ccatcgtggg agatgggaaa agactacaca cagcaattcg ttgtttcact
     5041 caggggattt ccatgcttat aaatcaatat gttgatttat aaccgtgagc taatagtagg
     5101 catacggact ttttgcgtcg atcagattcg tctcattagt ttaaaaactg taaagaattt
     5161 gcgacaggta ctaccagctg aatggcggta agcaggaaaa tatggggaag agcaagcaaa
     5221 tatcaattag cggtgattaa caatcaggcg gcagagccgc ctgatgagaa attacttatt
     5281 cggtttctcg atttcgccaa gttcttcgag aatttcttcc ggttggtccg tcggtggcgg
     5341 gacttcttcc acccacgcgg caaacagacg ccaggaaacg gctaacagaa ccggaccaat
     5401 aaacagaccg atcatcccga aagcaatcaa accaccaata acgccagaga ggatcaggat
     5461 cagcggtaaa tcggcaccca tgcgaattaa cattgggcgg atgacgttat ccagcgtgcc
     5521 aaccacaccg ctccacacta acaataccgt tccccaggtg gtatcgccgg tccagtagag
     5581 ccagataatc gccggaatca gtaccggcaa cgggccaagc tggacaaggc aggagaggat
     5641 cattaacacc gttagcaacg ttgcataagg tacgccggat acggcgaggc cgataccgcc
     5701 aagcactgcc tgtactaacg ccgtcaccac cacacccagc gccaccgcgc ggatagcctg
     5761 tgccgccagc agcacggcgg catctccgcg aacgcctgcc agacgggttg caaaatggcg
     5821 aatgccttgt gccacctgtt caccgcgcca gtacagcagg gcactgaaga gaagcatcag
     5881 cgcacaatgc accataaagc gcccgatatg cgccgcctgc ccaacgaacc aggtggtggt
     5941 ggtgccaata taagggcgga ctttcgccat gatcgccgta ccccccatat ccagcaagtt
     6001 gtgccagcct gcatacagct tcgcgccaat caccggaatg gtattaagcc acgccagatc
     6061 gggtaacgtc atgtcgccgc tggaaatggc tttaattagc gggccgctgc cgtcgacgat
     6121 actgttaacc agcaaagcaa tagggatgat aaacaccatc actaataaca gcgtcatcac
     6181 cagaacggcg agagagcggc ggccaaacat gatcttttgc aaacgtaaca ataccggcca
     6241 ggtggcgata accaccgtac cggcccatgc aaagccgaga ataaagggtt gaacaatcca
     6301 cagacatgcc acaatcatga tggctaaaaa cagcaccgaa agcagaattt gtgcgacatc
     6361 cctgggctga cgaacattta ccataactac ttttcacctt ttttgtgcgt caaatcgtcg
     6421 acgcgacgtg aacacgcgaa actcacctgc ataatgatga gcaatttcag cggtttttaa
     6481 caggccgatt ctgcccttaa ttctgatggg cgtgttagaa aaaaatgtgg tacaacaact
     6541 caggcaacac gcaaacgatt tactcgtcgt atttcaagtt gcatgtgctg cggctacgtt
     6601 cgttcacccc agtcacttac tattgtaagc tcctggagtt cactcgcttg ccgccttcct
     6661 gcaactcgaa atccatagag taaataaaca tcacctttca gtgcgctatt gcagccgcct
     6721 gaaagaatag tgtatcgacc tacatcacag accgcaggaa agggtcaata taatgattcc
     6781 acagatttcc ctggcacccg gcgtcgttca actggtgctt aattttttgc aagagctgga
     6841 gcaacaaggt tttaccggcg atgcggcgac aagttatgcc gatcgtctga caatgtcgac
     6901 cgacaacagt atttaccaac ttctccccga tgcggtggta tttccgcgtt caaccgcaga
     6961 tgtggcgctg atcgcccgtc ttgccgcgca ggaacgctat tcatcgctga tctttacccc
     7021 ccgcggcggc ggcaccggca ctaacggtca ggcgctcaac caggggatta ttgttgatat
     7081 gtcccgccat atgaaccgca tcatcgaaat taaccctgaa gagggctggg tgcgcgttga
     7141 ggccggggtg ataaaagacc aactcaatca gtacctgaaa ccgttcggct acttttttgc
     7201 gccggaactt tcgaccagca accgggcaac gctcggtggg atgatcaata ccgatgcatc
     7261 cggtcaggga tcgctggtct atggcaaaac gtcagatcac gtacttggcg tacgcgcggt
     7321 gttgttgggg ggcgatattc tcgatacgca acctttaccc gtcgagctgg cggaaacgct
     7381 gggtaaatcc aataccacaa tcgggcgaat ttataacacg gtttatcaac gttgccgtca
     7441 gcaacgccag ttaatcatcg acaacttccc caaacttaac cgctttctta ccggttacga
     7501 tctgcgtcat gtctttaacg atgagatgac cgagttcgac ctgacgcgca ttctgacggg
     7561 ttcagaaggg acgctggcct ttattaccga agcgcggctg gatattacgc gcttgcctaa
     7621 agtgcgccgt ctggtgaatg tcaaatatga ctcttttgac tctgcgctgc gtaacgcgcc
     7681 gtttatggtt gaggcgcggg cgctttcggt agagacggtg gactcaaaag tgctgaatct
     7741 ggcgcgggaa gatattgtct ggcattccgt cagcgagttg attaccgata tgcctgacaa
     7801 agagatgctc ggactgaaca ttgtggaatt tgctggtgat gatgaggcgc tgattgacga
     7861 gcgagttaat gcactctgtg tgcggcttga tgagctgatt gccagccaac aggcaggtgt
     7921 gattggctgg caggtgtgcc gcgagctggc gggggttgaa cgtatctatg cgatgcgcaa
     7981 aaaagccgtt ggtctgcttg gcaatgccaa aggtgccgct aagccaattc cgtttgctga
     8041 ggatacctgc gtaccgccgg aacatctggc cgattatatt gctgaatttc gcgcgctgct
     8101 cgacagccac ggcttaagct acggtatgtt cggtcacgtc gacgcaggtg tcttgcacgt
     8161 ccgtccggca ctggatatgt gcgatccgca acaagagatt ttgatgaagc aaatctctga
     8221 tgacgtggtg gcgctgactg cgaaatacgg tggtttgttg tggggcgagc acggcaaagg
     8281 ttttcgcgct gaatacagcc cggcgttttt cggtgaggaa ctttttgcag aactgcgcaa
     8341 agtgaaagcg gcatttgacc cgcataaccg actcaaccca gggaagattt gcccgccaga
     8401 aggtctcgat gcgccgatga tgaaagtgga cgcggtgaag cgcggtacat tcgatcggca
     8461 gatccccatt gcggtacgcc agcagtggcg cggtgcgatg gagtgtaacg gcaacggttt
     8521 atgcttcaac tttgatgccc gtagtccgat gtgtccgtcg atgaagatca cccagaaccg
     8581 gattcattca ccgaaagggc gcgcaacgct ggtgcgtgaa tggctgcgct tgttggcgga
     8641 tcgcggcgtt gacccactca aactggaaca agaactgcct gaatccggcg tcagtttgcg
     8701 gacgttaatt gcccgcacgc gcaatagctg gcatgcgaat aaaggcgaat atgacttctc
     8761 acacgaagtc aaagaggcga tgtcgggctg tctggcctgt aaagcttgtt cgacccagtg
     8821 ccccatcaaa attgatgtgc cggagtttcg ctctcgtttt ctgcagctct atcacacccg
     8881 ttatttacgc ccgcttcgcg accacctcgt tgctacggtc gagagctacg caccgctgat
     8941 ggcacgcgcg ccgaaaacct ttaacttctt cattaaccag ccgctggtgc gcaaactctc
     9001 ggaaaaacat atcggcatgg ttgatttgcc actgctgtcg gtcccctcgc tacaacaaca
     9061 aatggtgggg catcgctcgg caaacatgac gctggaacag cttgaagctc tcaatgcaga
     9121 gcagaaagcg cgcacggtgt tggtggtgca ggaccccttt accagctatt acgatgcgca
     9181 agtggtggcg gattttgtcc gtctggttga aaaattaggt ttccagcctg tgttactgcc
     9241 attttcgcca aatggcaaag cccagcatat caaaggcttt cttaatcgtt ttgcgaagac
     9301 ggcgaaaaag acggcggatt tcctcaaccg tatggctaag cttgggatgc caatggtggg
     9361 cgtcgatccg gcgctggtac tttgttatcg cgatgaatat aaactggcgc tgggcgagga
     9421 acgtggcgag tttaacgtct tacttgcgaa tgaatggctg gcaagcgcac ttgagtcaca
     9481 tccagtggct acagtcagcg gtgaatcatg gtatttcttt ggtcactgta ccgaggttac
     9541 cgctttgccg ggcgcgccag cacaatgggc cgcgatattt gcccgttttg gcgcgaaact
     9601 ggaaaatgtc agcgtgggtt gctgcggcat ggcagggact tacggacatg aagcgaaaaa
     9661 ccatgaaaat tcgctcggga tctatgagtt atcctggcat caggctatgc agcgactgcc
     9721 gcgtaaccgc tgtctggcga ccggatattc ctgccgtagc caggtaaaac gggttgaagg
     9781 cacgggggta cgccatcctg tgcaggcttt actggagatt attaaatgat atggaaacgg
     9841 aaaatcaccc tggaagcact gaatgctatg ggtgaaggaa acatggtggg gttcctggat
     9901 attcgctttg aacatattgg tgatgacacc cttgaagcga caatgccagt agactcgcgg
     9961 acaaagcagc ctttcgggtt gctgcatgga ggagcatccg tggtactggc cgaaagtatc
    10021 ggttccgttg ccggttattt atgtaccgaa ggtgagcaaa aagtggttgg tctggaaatc
    10081 aatgctaatc acgtccgctc ggcacgagaa gggcgggtgc gcggcatatg taaaccgttg
    10141 catctcggtt cgcgtcacca ggtctggcag attgaaatct tcgatgagaa agggcgtttg
    10201 tgctgttcgt cacgattgac gaccgccatt ttgtgacgtt gtcaccagaa aagtgtgacg
    10261 catattgctt ttggtcatta tctgcacagt aaacggtggt atactgtgca ggtcttgtct
    10321 aaaagcgagg atgctatgtc tactcaactt gatcccaccc agctggccat tgaattttta
    10381 cgccgtgacc agagcaatct ttcacctgcc caatatctca aacgtctgaa acaactagaa
    10441 cttgaatttg ctgacctgct tactctctct tctgctgaat taaaagaaga aatctacttt
    10501 gcctggcgtc tgggcgtgca ttgatcctga cttaatgccg caagttcgcc aaatcgacct
    10561 ggattaacct ccgtccaggt tcgatgacct tcttaatact gcttatctac cgcctgatat
    10621 tgttcaaaaa taatactaat cgcagtttgt gttaaaacgg cgggttagct ttatgagatt
    10681 attcttatcg ccccttcaag agctaagcca ctgagagtgc cggagataag cgccggatgg
    10741 ggtagaaacc cttaagcctg tgtcgcacag acttaagggt tttcttattt ctgaacgctg
    10801 cactttttta cctatctctt aaatagtcat tttcaataca actttttctt tttcatcaca
    10861 gccttaatgc tccttagata ataaccatta tctaacaatg agatacctaa ttcttagcgt
    10921 gcctgttaac ccacacatca ggggctatgc ttattaaaag caggcaaaaa gaatgtttta
    10981 cggtaaagcc cctgcgtttg ctgggttgaa ctgataatca ttatcactaa catgctgtta
    11041 tacgctgaaa gcgatgaagt gaggtaaata gatggacatg cattcaggaa cctttaaccc
    11101 acaggatttc gcctggcaag gcttaacgct gacacccgca gcggcgatac acatccgcga
    11161 gctggtggca aagcagccag gtatggtcgg cgtgcgctta ggtgtgaagc aaacgggctg
    11221 cgcgggcttt ggctatgtgc tcgacagtgt tagcgagccg gacaaagacg atctgctgtt
    11281 tgaacacgac ggcgcgaagc tgtttgtccc gctgcaatcg atgccgttta ttgatggcac
    11341 ggaagtcgat ttcgttcgtg aaggacttaa tcagatattc aaatttcaca accctaaagc
    11401 ccagaatgaa tgtggctgtg gcgaaagctt tagggtatag gcggtactat gtctcgtaat
    11461 actgaagcaa ctgacgatgt caaaacctgg accggcggcc cgctgaacta taaagaagga
    11521 ttcttcaccc agttagccac cgatgagctg gcaaagggga taaacgaaga ggtggtgcgc
    11581 gcaatttcgg cgaagcgtaa tgagccggag tggatgctgg agtttcgtct aaacgcctat
    11641 cgcgcatggc tggagatgga agaaccgcac tggttgaaag cgcactacga caagctgaat
    11701 tatcaggatt acagctacta ctcagcacca tcgtgcggta attgtgacga cacttgcgcg
    11761 tctgaacctg gcgcggtgca gcaaactggc gcgaacgcct ttttaagtaa agaggtggag
    11821 gcggcgtttg agcagttggg cgttcccgtg cgggaaggca aagaggtggc ggtggatgcc
    11881 attttcgact ctgtttcggt tgccaccact tatcgcgaaa aactggcgga gcagggaatt
    11941 attttctgtt cctttggtga ggcgatccac gatcacccgg aactggtgcg taaatatctc
    12001 ggcaccgtgg tgccggggaa tgacaacttc tttgcagcac ttaatgcggc ggtagcctct
    12061 gacggtacgt ttatttatgt gcctaaaggc gtgcgctgcc cgatggaact ttccacctat
    12121 tttcgcatta acgcggaaaa aaccgggcag tttgagcgca caattctggt ggccgacgaa
    12181 gacagctacg tcagctacat tgaaggctgt tccgctccgg tgcgtgacag ctatcagttg
    12241 cacgcggcag tggtggaagt catcatccat aaaaacgccg aggtgaaata ttccacggta
    12301 caaaactggt tccctggcga taacaacacc ggcggtattc tcaacttcgt caccaagcgt
    12361 gctttgtgcg aaggcgaaaa cagcaaaatg tcatggacgc aatcagaaac cgggtcagcg
    12421 attacgtgga aatatcccag ctgcattttg cgcggcgata actccattgg tgagttttac
    12481 tcagtggcgc tgaccagcgg tcatcagcaa gcggataccg gcaccaagat gatccacatc
    12541 ggtaaaaaca ccaaatcgac cattatctcg aaagggatct ctgccggaca tagtcagaac
    12601 agttatcgcg gcttagtgaa aatcatgccg acggcaacca atgcgcgcaa tttcactcag
    12661 tgcgactcaa tgctgattgg cgctaattgt ggggcgcata ccttcccgta tgtcgaatgt
    12721 cgcaataaca gcgcacaact ggagcacgag gcaacgacat cacgtattgg tgaagatcaa
    12781 ctgttttact gcctgcaacg cgggatcagc gaagaagacg ccatctcgat gattgttaac
    12841 ggtttctgca aagacgtgtt ctcagaactg ccgctggaat ttgccgttga agcacaaaaa
    12901 ctcctcgcca tcagtcttga acacagcgtc ggataaggaa taaacatgtt aagtattaaa
    12961 gatttacacg tcagcgtgga agataaagct atcctgcgtg gattaagcct cgacgttcgt
    13021 cccggcgaag ttcacgccat tatggggcca aacggttcgg gcaaaagtac cttatcggca
    13081 acgcttgccg ggcgcgaaga ttatgaagtg acgggcggca cggttgagtt caaaggcaaa
    13141 gatttgcttg cactgtcgcc ggaagatcgt gcaggcgaag gcatctttat ggccttccag
    13201 tatccggtgg agattccagg cgtcagtaac cagtttttcc tgcaaacggc acttaatgca
    13261 gtgcgcagct atcgcggcca ggaaacgctc gaccgctttg attttcagga tttgatggaa
    13321 gagaaaatcg ctctcctgaa gatgccggaa gatttattaa cccgttcggt aaacgttggt
    13381 ttttccggcg gcgagaaaaa gcgcaacgat attttgcaaa tggcggtgct ggaaccggag
    13441 ttatgcattc ttgatgagtc ggactccggg ctggatattg acgcattaaa agtggtcgcc
    13501 gatggcgtga actcgctgcg tgatggcaag cgctcattca tcattgttac gcactaccaa
    13561 cgcattctcg actacatcaa gcctgattac gttcatgtgc tatatcaggg acgaattgtg
    13621 aaatccggcg atttcacgtt ggtcaaacaa ctggaggagc agggttatgg ctggcttacc
    13681 gaacagcagt aatgcgctgc aacagtggca tcacttgttt gaagctgaag ggacaaaacg
    13741 ctcaccgcaa gcacagcagc atttacaaca attgctgcgt accggactgc cgacacgtaa
    13801 acatgaaaac tggaaatata cgccgctgga agggctgacc aatagtcagt ttgtcagcat
    13861 tgcgggagat atatccccac agcagcgtga tgccttagcg ttaacgttag acgccgtgcg
    13921 gctggtgttt gtcgatggac gttacgtgcc cgcgctgagc gatgcaattg aaggcagcgg
    13981 atatgaagtg agcattaacg acgaccgtca ggggttaccc gacgctattc aggcggaagt
    14041 gtttctgcat ttgacggaaa gcctggcaca aagcgtgacg catatcgccg tgaagcgcgg
    14101 tcaacggccg gcaaagccat tgctgttaat gcatatcacc cagggcgtgg caggtgaaga
    14161 ggtgaacact gcccattacc ggcatcatct ggatctggcg gaaggtgccg aagcaacggt
    14221 gatcgaacat tttgtcagcc ttaatgatgc tcgtcatttt accggggcac ggttcactat
    14281 caatgtcgca gcgaacgccc acttgcagca tatcaagctg gcgtttgaaa acccggtcag
    14341 tcaccacttt gcccataacg atttgttgct ggctgacgat gccaccgcat ttagccatag
    14401 tttcctgctg ggtggcgcag tgttacgaca caacaccagt acgcaactca atggcgaaaa
    14461 cagcacgctg cggatcaata gcctggcgat gccggtgaaa aacgaggtgt gtgatacccg
    14521 tacctggctg gaacacaata aaggtttttg taacagccga cagttgcaca aaactatcgt
    14581 cagcgacaaa ggccgcgcgg tatttaacgg tttgatcaac gtcgcgcagc acgccatcaa
    14641 aacggatggt cagatgacca acaataatct gctgatgggc aaactggcgg aagtggatac
    14701 aaaaccgcag ctggaaatct atgcagatga tgtgaaatgc agccacggcg cgacggtggg
    14761 gcgtattgat gatgaacaga tgttctatct gcgctcgcgc gggatcaatc agcaggatgc
    14821 ccagcagatg atcatttacg cctttgctgc cgaactgacg gaagcactgc gtgatgaggg
    14881 gcttaaacag caggtgctgg cccgaatcgg tcaacggctg ccaggaggtg caagatgact
    14941 ttttccgtcg acaaagtgcg ggccgacttt ccggtgcttt cgcgtgaggt aaacggtttg
    15001 ccgctggctt atctcgacag cgccgccagt gcacagaaac cgagccaggt gattgacgcc
    15061 gaggccgagt tttatcgtca tggctacgcg gcggtgcatc gcggtattca taccttaagc
    15121 gcccaggcga ccgagaaaat ggagaacgtg cgcaagcggg catcgctgtt tattaatgcc
    15181 cgttcggcgg aagagctggt gtttgtccgc ggcacgacgg aagggatcaa tctggtcgcc
    15241 aatagctggg gcaacagcaa tgtgcgggcg ggcgataaca tcatcatcag tcagatggag
    15301 caccacgcta acattgtccc ctggcagatg ctttgcgcac gcgttggcgc agagctgcgt
    15361 gtgatccctc tcaaccccga cggtacgctg caactggaga cgctgcctac gctgtttgat
    15421 gagaaaactc gcctgctggc aattactcat gtctccaacg tgcttggcac agaaaatcca
    15481 ctggcggaaa tgatcacgct tgcgcaccag catggcgcaa aagtgctggt ggatggcgct
    15541 caggcggtga tgcatcatcc ggtggatgtt caggcgctgg attgcgactt ttacgtgttc
    15601 tccgggcata aactgtatgg ccccaccgga attggcattc tttatgtgaa agaagccttg
    15661 ttgcaggaga tgccgccgtg ggaagggggc ggttctatga tcgccaccgt cagcctgagt
    15721 gaaggcacta cctggaccaa agcaccatgg cggtttgaag ccggtacacc caataccggg
    15781 ggcatcattg gtcttggcgc ggcgctggag tatgtttcgg cgctggggct taatagcata
    15841 gccgagtatg aacagaatct gatgcattat gcgctatcac agctggaatc tgtaccggat
    15901 ctcactctct atggccaaca aaacaggctt ggcgttattg cttttaatct cggtaaacac
    15961 cacgcctatg atgttggcag ttttctcgat aattacggca ttgctgtgcg taccggacat
    16021 cactgcgcta tgccattaat ggcctattac aacgtccctg cgatgtgtcg ggcgtcgctg
    16081 gccatgtata acacccatga agaagtggag cgtctggtga ccggcctgca acgtattcac
    16141 cgtctgctgg gataacaggg aggcactatg gctttattgc cggataaaga aaaattgctg
    16201 cgtaattttt tacgctgcgc caactgggaa gagaaatatc tctacattat tgagctgggc
    16261 cagcgtctgc cagaattacg cgccgaagac ggaagcccac aaaatagcat tcagggctgc
    16321 cagagtcagg tgtggattgt catgcgccag aatgcgcagg gaattattga attgcagggc
    16381 gacagcgatg cggcgattgt gaaagggctt attgcggtcg tctttattct ctacgatcag
    16441 atgacgccgc aggatattgt caatttcgat gtgcgtccgt ggtttgaaaa aatggcgctc
    16501 acccaacatc tcaccccatc tcgttcacaa ggtctggaag cgatgattcg cgcaattcgc
    16561 gccaaagccg ctgcacttag ctaaactaaa gggacagctt tcatccggtt ggctcacatt
    16621 gccggatggt caatgtttac actgaccggc acaggagtgt tttggtttca ggtgaacata
    16681 agcaccatcc gacgtttcag cgtgagtctc cggcaaatac aggaggttta caatgaaacg
    16741 cgcgtctttg cttacactca cgcttatcgg cgcttttagc gccatccagg ctgcctgggc
    16801 ggttgattat ccgctaccac caaccggaag ccgactggtt gggcaaaatc aaacgtatac
    16861 ggtgcaagaa ggggataaaa accttcaggc tattgcccga cgttttgata ctgcagcaat
    16921 gttgatcctt gaagccaata acactatcgc cccggtgcca aaacctggta cgacgataac
    16981 tattccttca caactgttat tacctgatgc accgcgtcag gggattatcg ttaaccttgc
    17041 agagctgcgc ctttattatt atccgccggg agaaaatatt gtgcaggttt atccaatagg
    17101 tattggattg caggggctgg aaacgccggt gatggaaacg cgtgttgggc agaaaatccc
    17161 taacccaacc tggacgccta cggcaggcat tcgtcagcgt tcgctggagc gtggcattaa
    17221 attaccgcca gtcgttcctg ccggaccaaa taacccgcta ggacgttacg cactgcgcct
    17281 cgcgcatggt aatggcgaat acctcattca tggcaccagt gcgccggaca gcgttggttt
    17341 gcgcgtcagt tcagggtgta ttcgcatgaa tgcaccggat attaaagcct tgttctccag
    17401 cgtgcggacg ggaacgccgg tgaaagtgat caacgaaccg gtgaaatatt ccgtggaacc
    17461 taacgggatg cgttatgttg aagtacatcg accactatca gcagaagaac agcaaaacgt
    17521 tcagacaatg ccatacacac tgccagcagg ctttacgcaa tttaaagaca ataaggctgt
    17581 agatcagaag ttagtcgata aagcgttgta tcgtcgggca gggtatccgg tttcggtgag
    17641 cagtggagca actcccgcag ccagcaatgc gccttcagta gagtcagcgc agaatggtga
    17701 accagagcaa gggaatatgt tacgcgcgac gcagtagtgg taaatagcag acaaaaaaat
    17761 ggcgcacaat gtgcgccatt tttcacttca caggtactat tacttgcggt atttagtagc
    17821 catgttgtcc agacgctggt tagcacgagc tgcgtcatct ttagcagcct gaacgtcgga
    17881 acgcattgcg ttcacgtcgt tgctcagctg gtcaacttta gcgttcagag tctgaacgtc
    17941 agaagacagc tgatcgattt tagcgttgct ggagcaacct gccagcagag tagaacccag
    18001 gattaccgcg cccagtacca gtttagtagc tttcattatt aataccctct agattgagtt
    18061 aatctccatg tagcgttaca agtattacac aaagtttttt atgttgagaa tatttttttg
    18121 atgggaaggc acttattttt gatcgttcgc tcaaagaagc atcgaacgct gggttttaga
    18181 taaaaaaatt gagagaaaac aggatgcttc catcggattc atcttaaata aagtgagatt
    18241 atatagtgaa atccgatttg attttttatt gcttctggtt atcgattaaa taaaaaaagc
    18301 gcccatcagg gcgcttcgat atacaaatta attcacaaaa gcaatattac aggacgtgaa
    18361 cagatgcggt gttagtagtg ccgctcggta ccagtgcacc agaaaccata actacgacgt
    18421 cacctttgtg tgccagaccg ctctgcagag ccagttcttt acccagacgg tagaaatcat
    18481 cagtagaagt gatctcttta accagctgcg gcacaacgcc tttgctcagt accaactgat
    18541 gagccgtttt ttcgttggtg gtcagtgcca ggatggtggc atccgggaag tatttacgta
    18601 ctgcgcgagc agatttaccg ccctgggtag caaccacgat cagcggagca tccagttttt
    18661 cagcagtttc aacggcacca cggcataccg cttcggtgat gcgcagttta cggttgtcat
    18721 tgttgaactc gagacggctg ttcatcacgc ggtcggtacg ttcgcagatg gtcgccatga
    18781 tagaaaccgc ttccagcggg tatttacctt ttgcggattc accagacagc atcactgcgt
    18841 cagtaccgtc gaggatggcg tttgcaacgt caccggcttc tgcgcgagtc gggcgtgggt
    18901 ttttgatcat ggaatccagc atctgggtcg cagtgataac gactttacgt gcacggatac
    18961 atttttcgat catcatcttc tgggcgaaga taacttcttc taccgggatt tctacaccca
    19021 ggtcgccacg cgcaaccatg atgccgtcag aggcttcgag gatttcgtcg aagttgttga
    19081 ggccttcctg gttttcgatt ttggagatga tgtggatgtt ttcgccgccg tgcgctttca
    19141 ggtgctcacg gatttcgata acgtcagaac gcttacgaat aaaggaagca gcaacaaagt
    19201 ctacgccttt ttcgcaacca aagatcaggt cctgtttgtc tttttcagcc agtgctggca
    19261 gagcaatgga aacgccaggc aggttcacac ctttgttttc gcccaggtca ccgttgttca
    19321 gcactttaca gataactttg ttaccttcaa tggcggtaac ttccataccg atcagaccat
    19381 cgtcaaccag tacggtgttg ccaacagaca ggtcagtagt gaaaccttca tacgttaccg
    19441 caaccatttc gctgttgccg ataacagatt tatcagtggt gaaagtaaag gtctgaccag
    19501 ctttcagaga aacatcgtta ccgccttcca gtttcatggt gcggatttcc ggacctttgg
    19561 tatcaagcag gatagcggcg gttttaccag ttttgctcat cacgttgcgc agattctgaa
    19621 tgcgctgacc gtgttctgca tagtcaccat gagagaagtt cagacgcata acgttcatgc
    19681 cagcgtccag cattttagct aacatctctt cagattcggt tttcggtccg atggtgcaaa
    19741 caattttggt ctttttcatg acagtcttag tctttaagtt gagaaggatg ggagaaactt
    19801 gctttctggg cgcactgccg gaaagcgaat ccggtattac gaaagtggtg gtgcctcgct
    19861 ctaaggatag gtgacatcga aaaagcgtgc agaggaatgt gtgcttgtgt ctcagcccaa
    19921 cgtgagtgtc tgtttttgag tcgttgagtt ctacagtcca aagtgctgaa accattcaag
    19981 agtcaattgg cgcgcattat acgctgaaat taatccaaaa ggaaaacaaa aacagcgttt
    20041 caaaaaatat acaacttgtt agacaataaa ttgttatcaa agatttaacg ttccagggaa
    20101 aaggttacgc aaaaacttcg ctgtttgcgc aacgtctggt gccgaagttg tcagtgtgag
    20161 gataagtgaa agttcatccg gtgtataaat atatcatcgc tcaattgcta cagttaaaat
    20221 aatttatcgc gttatttgtc tgattttgtt tgatttttgt ttaattggtt agattgatat
    20281 atgctga
//
LOCUS       DAJTSA010000075        20219 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000075 DAJTSA010000000
VERSION     DAJTSA010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.075"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            75..227
                     /gene="hokA"
                     /locus_tag="OO369_002822"
     CDS             75..227
                     /gene="hokA"
                     /locus_tag="OO369_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR5983035.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(414..626)
                     /gene="cspA"
                     /locus_tag="OO369_002823"
     CDS             complement(414..626)
                     /gene="cspA"
                     /locus_tag="OO369_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR5983036.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(907..1197)
                     /locus_tag="OO369_002824"
                     /pseudo
     CDS             complement(907..1197)
                     /locus_tag="OO369_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1631..2341
                     /locus_tag="OO369_002825"
     CDS             1631..2341
                     /locus_tag="OO369_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR5983037.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYLAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="OO369_002826"
     CDS             complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="OO369_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR5983038.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3469..4119)
                     /locus_tag="OO369_002827"
     CDS             complement(3469..4119)
                     /locus_tag="OO369_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR5983039.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4282..6615
                     /gene="bisC"
                     /locus_tag="OO369_002828"
     CDS             4282..6615
                     /gene="bisC"
                     /locus_tag="OO369_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCR5983040.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6584..7024)
                     /locus_tag="OO369_002829"
     CDS             complement(6584..7024)
                     /locus_tag="OO369_002829"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5983041.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7021..7584)
                     /gene="tag"
                     /locus_tag="OO369_002830"
     CDS             complement(7021..7584)
                     /gene="tag"
                     /locus_tag="OO369_002830"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR5983042.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7742..8440
                     /locus_tag="OO369_002831"
     CDS             7742..8440
                     /locus_tag="OO369_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5983043.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRSWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8669..9877
                     /locus_tag="OO369_002832"
     CDS             8669..9877
                     /locus_tag="OO369_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5983044.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10201..11892
                     /gene="eptB"
                     /locus_tag="OO369_002833"
     CDS             10201..11892
                     /gene="eptB"
                     /locus_tag="OO369_002833"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR5983045.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            11984..12060
                     /locus_tag="OO369_002834"
     tRNA            11984..12060
                     /locus_tag="OO369_002834"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12018..12020,aa:Pro,seq:cgg)
     gene            12804..14411
                     /gene="dppA"
                     /locus_tag="OO369_002835"
     CDS             12804..14411
                     /gene="dppA"
                     /locus_tag="OO369_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR5983046.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14719..15738
                     /gene="dppB"
                     /locus_tag="OO369_002836"
     CDS             14719..15738
                     /gene="dppB"
                     /locus_tag="OO369_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR5983047.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15748..16650
                     /gene="dppC"
                     /locus_tag="OO369_002837"
     CDS             15748..16650
                     /gene="dppC"
                     /locus_tag="OO369_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR5983048.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16661..17644
                     /gene="dppD"
                     /locus_tag="OO369_002838"
     CDS             16661..17644
                     /gene="dppD"
                     /locus_tag="OO369_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR5983049.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17641..18645
                     /gene="dppF"
                     /locus_tag="OO369_002839"
     CDS             17641..18645
                     /gene="dppF"
                     /locus_tag="OO369_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR5983050.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18675..19946)
                     /locus_tag="OO369_002840"
     CDS             complement(18675..19946)
                     /locus_tag="OO369_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5983051.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         4843 a         5008 c         5306 g         5062 t
ORIGIN      
        1 agtgaggcta ccctatgcct gaacaccata aggttagcct cttactcgtt ggaaatcaac
       61 acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg atttgtttca
      121 cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat attaaacagg
      181 agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag tcttcgacgg
      241 gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccat atttattctt
      301 cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga aaaaaaataa
      361 aaaaatcccc gccaaatggc agggatctta gattctgtgc ttttaagcag agattacagg
      421 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      481 tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacaca
      541 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      601 acgataccag tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc
      661 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      721 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      781 accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt tttcatattt
      841 atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat
      901 aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa
      961 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac
     1021 tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt ttcataactg
     1081 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
     1141 aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga
     1201 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
     1261 ggaaaagcag acacagcggg gtgaagtcag ggcatgctct ggctatgatt cgattcagcc
     1321 tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttat
     1381 cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta aatctctggc
     1441 tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt aaccttgttt
     1501 cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc aagcctgtcc
     1561 tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag
     1621 gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt
     1681 attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt
     1741 tatcgacttc ctgcaaaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc
     1801 cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc
     1861 tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca gcgttaacgc
     1921 aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaatggttc
     1981 gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg ctgcgcactc
     2041 tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca ccaaagtggt
     2101 aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt tcacgcaaca
     2161 actggtgatg gttggagact accttgctca gcaaggtact caggtgagct ttgtcgcaaa
     2221 tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga ttgcgccatt
     2281 accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata
     2341 aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcagc ttagtccgcg
     2401 acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat caaattatcc
     2461 acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtact
     2521 gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag
     2581 acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag tgcattttcg
     2641 tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat
     2701 tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag gcaaacgaaa
     2761 tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct
     2821 tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa gtgcacacgt
     2881 tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg
     2941 tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt tacccgttct
     3001 gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg
     3061 gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg
     3121 acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt cggcattttt
     3181 tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc ttctgcaaaa
     3241 attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg aacggtgaaa
     3301 tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc
     3361 ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga
     3421 aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt acagcgggct
     3481 taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt tgctggcgat
     3541 tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc cctgggtgat
     3601 caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca ggtcgtgacc
     3661 ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact ctttcagcac
     3721 cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggagcgc tgctgctgtc
     3781 gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac
     3841 accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataacccac
     3901 gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac cgcgatcttt
     3961 cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac ctgcgccgat
     4021 agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc cagataccgc
     4081 cagagcgcca ctcactacgg cggcaagtaa gataaacacg tttcttcatt gttaatcctt
     4141 aataaccttt ttattctttg tcacgggttc cgtggcggga gattatgccg cgtgaacatg
     4201 aagattattc ctgggaatac tcggaaattt gtaagtaata tttaactgct caatacatct
     4261 aacctttcag gagtcttcgg tttggccaac tcatcctcac gatattccgt tctgactgcc
     4321 gcccactggg ggcccatgct ggttgaaacc gacggcgaaa ccgtgtttag ctcgcgtggc
     4381 gcgttagcca caggaatgga aaactccttg cagagcgcgg ttcgcgacca ggttcacagc
     4441 aatacgcggg tacgatttcc aatggtgcgc aaaggctttc tcgcgtcacc ggaaaacccg
     4501 caaggcattc gtgggcagga tgaatttgtt cgcgtgagtt gggatgaggc gctggatctt
     4561 attcaccaac aacataaacg cattcgtgag gcttatggtc cggcatcgat ttttgctggt
     4621 tcctacggct ggcgttcaaa cggcgtgctg cataaggcct cgacattatt acaacgctat
     4681 atggcgctgg caggcggtta taccgggcat ctgggggatt attcgaccgg tgcggcacag
     4741 gcgatcatgc cgtatgtcgt aggtggcagc gaagtttatc aacagcaaac cagttggccg
     4801 ctggtgctgg aacatagcga tgtcgtggtg ttgtggagtg ctaacccact caatacgctg
     4861 aaaattgcgt ggaatgcatc cgatgagcag gggctttctt acttttccgc actgcgtgac
     4921 agtgggaaaa agctgatctg cattgatcca atgcgatcgg aaaccgtcga tttctttggc
     4981 gataaaatgg agtgggtggc accgcacatg ggcaccgatg ttgcgctgat gctggggatc
     5041 gcccatacgc tggtggaaaa tggttggcac gacgaagcgt ttctggcgcg ttgcaccaca
     5101 ggttatgccg tcttcgcctc ttatttgctg ggcgagagtg acggaatagc gaaaaacgcc
     5161 gaatgggcgg cagagatttg tggtgttggc gcagcgaaaa tccgcgagct ggcggctatt
     5221 ttccaccaaa ataccaccat gctgatggca ggctggggaa tgcagcgcca acagtttggt
     5281 gagcaaaaac actggatgat cgtcacgctg gcagcaatgt tggggcaaat cggcacaccc
     5341 ggcggcggtt ttggtctttc ttaccatttt gccaatggtg gtaaccccac gcggcgttct
     5401 gcggtgctct cttccatgca gggcagcttg ccgggtggct gcgatgcggt ggataaaatc
     5461 cctgttgccc gcattgttga agcactggaa aaccctggtg gcgcatatca acacaacggt
     5521 atgaaccgac atttcccgga tattcgtttt atctggtggg cgggcggtgc caactttact
     5581 catcatcagg ataccaatcg tctgatccgt gcctggcaaa aaccggagct ggtggtgatc
     5641 tctgaatgct tctggacggc ggcggcgaaa cacgcggata ttgttctgcc tgcgactacc
     5701 tcttttgagc gtaatgatct caccatgacc ggtgattaca gtaatcagca tctggtgccg
     5761 atgaagcaag tggtgccgcc acgctatgaa gcgcgtaatg attttgatgt ttttgccgag
     5821 ttgagtgaac gttgggagaa gggcggctat gcgcggttta cggaaggaaa aagtcagctg
     5881 caatggctgg aaacgtttta taacgttgcc cggcagcgcg gggcaagcca gcaggttgaa
     5941 ttgccgccat ttgctgagtt ctggcaagcc aaccagttaa ttgagatgcc ggaaaacccg
     6001 gacagcgagc ggtttattcg cttcgctgat ttttgccgcg atccgctggc gcatccgtta
     6061 aaaaccgcca gcggcaagat tgaaatcttc tcacagcgta ttgccgatta cggttacccg
     6121 gattgccctg ggcatccaat gtggctggag ccggacgaat ggcagggcaa tgccgaacca
     6181 gaacagttgc aggtactttc tgcccatccg gcgcaccgcc tgcacagcca gctgaattac
     6241 agttctctgc gcgaattgta cgcggtagca aaccgtgagc ctgtcaccat tcatcctgac
     6301 gatgctcagg cgcgcggcat aaccgaaggg gatatggtgc gggtgtggaa ctcccgtggg
     6361 cagatccttg ccggagcggt cattagcgag ggaattaaac ctggcgtgat ttgcattcac
     6421 gaaggggcat ggccggatct ggatttaacc gctgacggta tttgtaaaaa cggcgcagtg
     6481 aacgtgctga ccaaagatct ccccagctcg cggctgggga atggctgtgc gggtaatacg
     6541 acgctggcat ggctggaaaa atacaacggt ccggaactga cacttacagc gtttgaacca
     6601 ccggccagct cataatccag gtgggtagtt gggtttcatc ctgccatgcg caatcgacaa
     6661 tgtgaaaacc ctgcgcacgg taaaaatcta tcgccggttg atttttttga taaacctcca
     6721 gcatcagatg gggatagcgc tgctgcacat actgcatcag cgccttacca ataccgcgtc
     6781 tgacggcctt cggtgcgaca aacattgctg ccagaaatcg gccttccata atgctgacaa
     6841 aaccgagaag cttaccgtcc tcttcccaga cccagttttg cgcgttggca agataggcat
     6901 cccgcaccag cggaatgcag tcacgccagt aattcgcttt tataaaggga tgcccccagg
     6961 ttgtactttc cagccacagt tcgaggatcg cggggagttc tgaacgttgc gcttcccgaa
     7021 tcatggttta tttcccagat agcaacagca gccaaccaca tgatcattca ccagcccaca
     7081 tgcctgcata aaggagtaac agattgtggt gccgacaaac ttaaaaccac gttttttcag
     7141 tgccttagat agggcgtcgg aggcggacgt agatgtggga atttcgctca acgttgtggc
     7201 ttgtgtcacc tgtggctgat gatttacaaa cgaccagaca aagtcgggaa acggttcgcc
     7261 gttctgttcc atttgcaggt acgcccgcgc attaccaata attgcctgaa ttttccctcg
     7321 atggcggata atcccggcgt cctgtaccag tctttcgaca tcctcttcct gcattgctgc
     7381 gaccttcacc ggatcgaact gatgaaagta ggcgcgatag ttttcgcgtt ttttgaggac
     7441 ggtgatccac gataacccag cctgctgccc ttcaaagcag atcatttcga acagtttttt
     7501 actgtcagtt tcaggcacgc cccactcatt atcatggtag gcaatataaa gcgggccctg
     7561 actcacccag ccgcaacgtt ccatactttc cctcgctatg tacccgatcg taaaaatttc
     7621 agccgctttt cttgacgcat cggtgaaaaa gtcaatattt aatacagggc tactatgccc
     7681 gatatccttc atacaatgac aataatattg ccgaattcgg ctcttccctg gagtcgtgtt
     7741 aatgatcata aaaaaaagcg gtggtcgctg gcagctaagc ctgctggcga gcgtggtaat
     7801 cagtgccttt tttctcaaca cagcttacgc ctggcaacaa gaatatatcg ttgatacgca
     7861 acccggacat tccacagagc gttacacctg ggatagtgat catcaacctg attacaacga
     7921 tattttgtcg caacgtattc aaagtagcca aagggcgctg ggactggaag tcaatctggc
     7981 ggaagaaact cctgtggatg tgaccagcag tatgagtatg ggctggaatt ttcctttata
     8041 tgaacaggtt acaaccggcc cggtcgcggc attacattac gatggcacaa ccacctcgat
     8101 gtataacgag tttggcgaca gtactaccac gctgaccgat ccgttatggc atgccagcgt
     8161 gagtagctta ggctggcgtg ttgactcccg gcttggcgat ctccgatcct gggcgcaaat
     8221 cagctataac cagcaatttg gcgagaatat ctggaaggcg caatcaggcc tgagccggat
     8281 gacggcgaca aaccagaacg gcaactggct ggatgtcacc gtaggcgctg atatgttgct
     8341 caatcaaaat attgccgcct atgctgcgct aactcaggca gaaaatacca ctaataatag
     8401 cgactatctg tatacgatgg gggttagcgc cagattttaa cgtaacagtc acaattgaaa
     8461 ccattgaata acaacagttg tggcgatagt gggtgctaac ttaccaaata ataaatttgg
     8521 tgaataattg tcgcgtcatt cattcctgaa ctaaggcatt tcattccgtt ctgatggcat
     8581 ttcatgccgt ttttccccaa gcataaaatg cacttcgtta tggttgtcgg cagagatttt
     8641 tcctttttat tactgcagga atactgccat gacaccttca aattatcagc gtacccgctg
     8701 gctgacactc atcggtacta tcattaccca gtttgcgctg gggtcggttt atacccggag
     8761 cctgtttaat ggcgcgcttt ctgccaagct ggatgcgccg gtaagccagg tcgctttctc
     8821 tttcggcttg ttaagtctgg ggctggcaat ttcgtcttct gttgcgggca aattgcagga
     8881 acgttttggc gttaaacgcg tcaccgtggc ttccggcatt ttgctgggat taggcttctt
     8941 cctgaccgcg cattccaaca acctgatgat gctgtggtta agcgccggtg tgctggtcgg
     9001 tctggcggat ggcgcgggtt acctgctgac gctctctaac tgcgtgaagt ggttcccgga
     9061 gcgtaaaggt ctgatctccg cgttcgctat cggttcttat ggtctgggta gcctgggttt
     9121 caaatttatc gacacgcagc tgctcgaaac ggtgggtctg gaaaaaacct ttgtgatttg
     9181 gggagcgatt gcgctggtga tgattgtttt cggcgcaacg ttaatgaaag acgcaccaaa
     9241 acaggaagtg aaaaccagca atggtgtggt ggagaaagat tacacgctgg cagagtcgat
     9301 gcgtaaaccg cagtactgga tgttagcggt aatgttcctg actgcctgca tgagcggcct
     9361 gtacgtgatt ggggtagcga aagatatcgc ccaaagtctg gcacaccttg atgtggtttc
     9421 cgcagccaat gcagtaacgg ttatttccat cgctaacctt tcaggtcgtc tggtgctggg
     9481 cattctgtct gacaaaattg cccgtatccg tgttatcacc attggtcagg tgatttcgct
     9541 ggtgggtatg gcggccctgc tgtttgcacc attaaatgca gtgacgttct ttgcagcgat
     9601 tgcctgcgtg gcatttaact ttggcggcac tattaccgtc tttccatcac tggtcagtga
     9661 gttcttcggc ctcaataacc tggcgaaaaa ctacggtgtg atttacctcg gtttcggtat
     9721 cggtagcatt tttggttcga ttatcgcctc actgtttggc ggtttctatg tgaccttcta
     9781 cgtgattttc gccctgctga ttctgtcatt ggcgctttct acgacgattc gtcagccaga
     9841 gcagaaaatg ttgcgtgagg cgcatggctc cctttaatgc tcagccgcga gctactgggc
     9901 ttcagcttcc gtgtgcatca aacgcatcaa cgaaatgtat aaacaatgac tcgtaaaatt
     9961 tgggccagtt aactggccct ttttactgct ttttgtaacg ctaaagtctc ttttcaaact
    10021 tgcatttttg taaatttgtg cttcatgcac actctttccc cacacttttt cgctttgctg
    10081 tggtctactt attcgcgcgt gtagatttta cttatctgac tacctccgca cttttcccct
    10141 gccgggcctg aaaagccgct aagcagggtg ttatcacctg tttgtccagg gtttgtttgc
    10201 atgagataca tcaaatcgat tacacagcag aagctgagct ttttgcttgc aatctatatt
    10261 ggccttttta tgaatggcgc ggttttttat cgccgcttcg gcagctatgc gcacgatttt
    10321 accgtctgga aaggcgtttc tgctgttgtt gaactggccg ccaccgtact ggtgaccttc
    10381 tttttactac gtcttctatc gctgtttggc cgccgcagct ggcgtattct ggcgtcgctg
    10441 gtggtgctct tttccgcagg tgccagctat tacatgacct tccttaatgt ggtcattggt
    10501 tatggcatca tcgcttccgt catgaccacc gatatcgacc tgtcaaaaga agttgttggt
    10561 ctgaacttta ttctctggtt aatcgccgtt agtgcattgc ctcttatcct tatctggaat
    10621 aaccgctgtc gctacacctt gctccgacaa ctgcgaaccc cagggcagcg tattcgcagc
    10681 ctggtggtcg tcgtactggc gggtattatg gtttgggcac cgattcgttt gctggatatc
    10741 cagcagaaga aagtggagag ggcgaccggc gttgatttgc cgagttatgg cggtgtcgta
    10801 gcgaactctt atctgccatc aaactggctt tctgcgttgg ggctgtatgc ctgggcgcgg
    10861 gtcgatgaat cttccgataa taattcattg cttaatccgg cgaagaagtt tacataccag
    10921 gctccacaaa acgttgatga tacttatgtc gtgtttatca tcggtgaaac cacgcgttgg
    10981 gaccatatgg gtattttcgg ctatgagcgt aataccacgc cgaaactggc ccaggagaaa
    11041 aatctggcgg cgttccgtgg ttactcctgt gataccgcaa ccaaactctc actgcgttgc
    11101 atgtttgtac gtcagggggg cgcggaagat aatccgcagc gcacattaaa agaacagaac
    11161 attttcgcgg ttctgaagca gttaggattc agttctgacc tctacgctat gcagagcgaa
    11221 atgtggttct acagcaacac gatggcagac aacattgctt atcgtgagca gattggtgcg
    11281 gagccacgta atcgtggcaa gccggtagat gatatgttgc tggtagacga aatgcagcaa
    11341 tcgctagggc gcaacccgga tggtaagcat ctgatcattc tgcataccaa aggttcgcat
    11401 tttaactaca cccagcgtta tccgcgtagc ttcgcgcagt ggaagccgga atgtattggt
    11461 gttgatagcg gctgtaccaa agcgcagatg atcaactcct atgacaactc ggtgacctat
    11521 gtggatcact ttatctccag cgtgattgat caggttcgcg ataagaaagc gattgtgttc
    11581 tacgcagctg accacggtga gtcaattaat gaacgcgagc acctgcacgg cacgccgcgt
    11641 gaactggcac cgccggagca gttccgcgta ccgatgatgg tctggatgtc agataaatat
    11701 ctggaaaatc cggccaatgc gcaggcgttt gcgcagctga aaaaagaagc cgacatgaaa
    11761 gtgccacgcc gtcacgtaga gctgtacgac accatcatgg gttgtcttgg ctatacttca
    11821 ccggatggtg gaattaacga aaacaacaac tggtgtcaca tcccgcagac aaaagtggca
    11881 gcggctaact aagcggcctg ctgactttct cgccgatcaa aaggcatttt gctattaagg
    11941 gattgacgag ggcgtatctg cgcagtaaga tgcgccccgc attcggtgat tggcgcagcc
    12001 tggtagcgca cttcgttcgg gacgaagggg tcggaggttc gaatcctcta tcaccgacca
    12061 aattcgaaaa gcctgctcaa cgagcaggtt tttttgcatt tgtagctcat gaggatgaga
    12121 acctccgggg gcagggaggc tcgactcgac gtaggcctga taagacgcgc cagcgtcgca
    12181 tcaggcaacg gcaccgcact tattgtcgga tgcggcgcga ccgccttatt cgaccaacac
    12241 accacacatt ttcctgccaa ctttgcgccc catatctcac tatccattaa cggatttgtg
    12301 acagaaacca ttgtcttttt ttatatttgc ttaaatcttt tttgcgttac ttatggctgg
    12361 cgctggcatt ttccgctgtg cgttttagcg ttcatggtat taatcgctca gatatccacc
    12421 ttttcctcag atttttttca ttaaatgcat aaaacttaat caccaaatgt tgctaaatat
    12481 taagctgatt gttctggtta tgttggagta gcacaaattt ctctgctgca aatagctgtt
    12541 tgaagttttt ttaatctttg tttgtggatt ctcaccgttg gtgtaaatcc cggttggctg
    12601 tattgacgtt ttcacattct gttgacagat tgtaggtcac gaggggcatt ttatggagga
    12661 tccgcactgt tacactgatg ttaattagta cggcatcccc acctcataac gttgacccga
    12721 ccgggcaaaa aacaaaaaag gtcaggcagc gacaacccac tgcaaagggt taaaacaaca
    12781 aacatcacaa ttggagcaga ataatgcgta tttccttgaa aaagtcaggg atgctgaagc
    12841 ttggtctcag cctggtggct atgaccgtcg cagcaagtgt tcaggctaaa actctggttt
    12901 attgctcaga aggatctccg gaagggttta acccgcagct gtttacctcc ggcaccacct
    12961 atgacgcctc ttccgtcccg ctttataacc gcctggttga atttaaaatc ggcaccaccg
    13021 aagtgatccc gggcctcgct gaaaagtggg aagtcagcga agacggtaaa acctatacct
    13081 tccatctgcg taaaggtgtg aagtggcacg acaataaaga attcaaaccg acgcgcgaaa
    13141 tgaacgccga tgacgtggtg ttctcgttcg atcgtcagaa aaacgcgcaa aacccgtacc
    13201 ataaagtttc tggcggcagc tacgaatact tcgaaggtat gggcttgccg gagctgatca
    13261 gcgaagtgaa aaaggtggac gataacaccg ttcagtttgt gctgactcga ccggaagcgc
    13321 cgttcctcgc tgacctggca atggacttcg cctctattct gtcaaaagaa tatgctgacg
    13381 cgatgatgaa agccggtaca ccggaaaaac tggacctcaa cccaatcgga accggtccgt
    13441 tccagttaca gcagtaccaa aaagattccc gtatccgcta caaagcgttt gatggctact
    13501 ggggcaccaa accgcagatc gatacgctgg tcttctctat tacccctgat gcttccgtgc
    13561 gttacgcgaa attgcagaag aatgaatgcc aggtgatgcc gtacccgaac ccggcagaca
    13621 tcgctcgcat gaagcaggat aaatccatca acctgatgga aatgccgggg ctgaacgtcg
    13681 gctacctctc gtataacgtg cagaaaaaac cgctggatga cgtgaaagtt cgccaagcac
    13741 taacctacgc ggtgaacaaa gacgcgatca tcaaagcggt ttatcagggc gcgggtgtat
    13801 cagcgaaaaa cctgatcccg ccaaccatgt ggggctataa cgacgacgtt caggattaca
    13861 cctacgatcc tgaaaaagcg aaagccttgc tgaaagaagc gggtctggaa aaaggtttct
    13921 ccatcgacct gtgggcgatg ccggtacaac gtccgtataa cccgaacgct cgccgcatgg
    13981 cggagatgat tcaggcagac tgggcgaaag tgggcgtgca ggccaaaatc gtcacctacg
    14041 aatggggtga gtacctcaag cgtgcgaaag atggcgaaca tcagacggta atgatgggct
    14101 ggactggcga taacggggac ccggataact tcttcgccac cctgttcagc tgcgccgcct
    14161 ctgaacaagg ttctaactac tcaaaatggt gctacaaacc gtttgaagat ctgattcaac
    14221 cggcgcgtgc taccgacgac cataacaaac gcgttgaact gtacaagcaa gcgcaggtgg
    14281 tgatgcacga tcaggctccg gcactgatca tcgctcactc caccgtgttt gaaccggtac
    14341 gcaaagaagt caaaggctat gtggttgatc cattaggcaa acatcacttc gaaaacgtct
    14401 ctatcgaata attaaaagcc atacaagact gatggcaaag gcaaaactgc ctgatgtgct
    14461 ccgcttatca ggcctacgaa aattctgcaa tgtattgaat ttgcacgatt ttgtaggccg
    14521 gataaggcgt taacgccgca tccggcataa acaaagcgca ctttgtcaac aatctgtata
    14581 cccggtggcg ctgtgcctct gcgtgcagcg ccatccggca gcaatacttc attctctgtc
    14641 cgacaagggc gggaattgat ttgtgagcaa tacagacacg cagttccagg ctgcgagtca
    14701 ctatagagaa tccgggttat gttgcagttt attctccgac gtttgggact cgtcatcccc
    14761 acgtttatcg gtattaccct tctcacattt gcctttgtcc acatgatccc gggcgatccg
    14821 gtgatgatca tggcaggcga acgtgggatc tcccctgagc gtcacgtgca gctgctggct
    14881 gaactcggct tagataaacc gatgtggcag cagtatctcc attacatttg gggcgtaatg
    14941 cacggcgatc taggcatttc aatgaaaagc cgaattccag tatgggaaga gttcgtgccg
    15001 cgctttcagg ccacgctgga acttggcgtc tgcgcgatga tttttgctac ggcagtcggt
    15061 attccggttg gtgtgctggc tgcggttaaa cgcggttcca ttttcgatca cactgcagtt
    15121 ggcctggcgc tgaccggcta ctcgatgccg atcttctggt ggggcatgat gctgatcatg
    15181 ctggtttcgg tgcactggaa cctgacgccc gtctccggtc gcgtgagcga tatggtgttc
    15241 ctcgatgact ccaatccgtt aaccggtttt atgctaatcg acaccgccat ctggggtgaa
    15301 gacggcaact ttatcgatgc cgtcgcccat atgatcttgc ccgccattgt gctgggtact
    15361 attccgctag cggtcattgt gcgtatgaca cgctcctcga tgctggaagt gctgggcgag
    15421 gattacatcc gcaccgcgcg cgccaaaggg ctaacccgca tgcgggtgat tatcgtccat
    15481 gcgctgcgta acgcgatgct gccggtagtg accgttatcg gcctccaggt gggaacattg
    15541 ctggcggggg cgattctgac cgaaaccatc ttctcgtggc ccggtctggg gcgctggttg
    15601 attgacgcac tgcaacgccg cgactatccg gtagtgcagg gcggcgtatt gctggtggcg
    15661 acgatgatta tcctcgtcaa cttgctggtc gatctgctgt acggcgtggt gaacccgcgt
    15721 attcgtcata agaagtaagg ggacatcatg tcacaggtta ctgaaaataa agtgattagc
    15781 gcaccggtgc cgatgacccc gttacaggag ttctggcact attttaaacg caacaaaggc
    15841 gcggtcgtcg ggctggttta cgtcgccatc gtgctgttca tcgcgatctt tgccaactgg
    15901 attgcgcctt ataacccggc ggatcagttc cgcgatgcgc tgctcgcccc gccagcctgg
    15961 caggaaggcg gcagtatggc acacttgctg ggcaccgatg acgtcggtcg tgatgtgctg
    16021 tcgcggctga tgtacggcgc gcgtttatcg ctgctggttg gctgtctggt ggtggtgttg
    16081 tcgctgatta tgggcgttat tctcggcctg atcgccggtt acttcggcgg cctggttgat
    16141 aacattatca tgcgcgtggt cgatatcatg ctggcgctgc caagtctgct gttggcactg
    16201 gtgctggtgg cgattttcgg cccgtcgatt ggtaacgccg cgctggcgct gaccttcgtt
    16261 gccttgccgc actatgtgcg cttaacccgc gccgccgtgc tggtggaggt gaatcgcgat
    16321 tacgtcaccg cctctcgcgt ggcaggtgcc ggggcgatgc gccagatgtt tattaacatc
    16381 ttcccgaact gccttgcgcc gctgattgtt caggcgtcgc tcggtttctc taacgccatt
    16441 ctcgatatgg ctgctcttgg tttcctcggc atgggggcac agccgccaac gcctgagtgg
    16501 ggcaccatgc tctccgacgt gttgcagttc gcgcaaagcg cctggtgggt cgtgaccttc
    16561 cctggtctgg cgatcctgtt gacggtgctg gcatttaacc tgatgggtga cggtctgcgt
    16621 gacgcgctcg atcccaaact aaagcagtaa gaggttcgag atggcgttat taaatgtaga
    16681 taaattatcg gtgcatttcg gcgacgaaag cgcaccgttc cgcgccgtag accgcatcag
    16741 ctacagcgta aaacagggcg aagtggtcgg gattgtgggt gagtccggct ccggtaagtc
    16801 ggtcagttca ctggcgatta tggggctgat tgattatccg ggccgcgtaa tggcagaaaa
    16861 actggagttt aacggccagg atttgcagcg tatctcagaa aaagagcgcc gcaacctggt
    16921 gggtgccgaa gtggcgatga tcttccagga cccgatgacc agccttaacc cgtgctacac
    16981 cgtgggtttc cagattatgg aagcgattaa agtgcatcag ggcggcaaca aaagtacccg
    17041 ccgtcagcgg gcgattgacc tgctgaatca ggtcggtatt cccgatccgg cttcgcgtct
    17101 ggatgtttac ccgcatcagc tttccggcgg catgagccag cgcgtgatga tcgccatggc
    17161 gattgcctgt cggccaaaac tgctgattgc cgatgaaccg accaccgcgc tggacgtgac
    17221 cattcaggcg caaatcatcg aactactgct ggagctacag cagaaagaga acatggcgct
    17281 ggtgttaatt acccatgacc tggcgctggt ggcggaagct gcgcataaaa tcattgtgat
    17341 gtatgcaggt caggtagtag aaacgggcga tgcacacgcc atcttccatg cgccgcgtca
    17401 cccgtatact caggcattgc tgcgtgcgct gccagaattt gctcaggaca aagaacgtct
    17461 ggcgtcgttg ccaggtgttg ttcccggcaa gtatgaccgc ccgaacggct gcctgcttaa
    17521 cccgcgctgc ccctatgcca ctgacagatg tcgcgctgaa gaaccggcgc tgaatatgct
    17581 cgctgatggg cgtcagtcca aatgccatta cccacttgat gatgccggga ggcccacact
    17641 atgagtacgc aagaggccac ctcgcaacaa ccgctgttgc aggctatcga cctgaaaaaa
    17701 cattatccgg tgaagaaagg catgttcgtg ccggaacgtc tggtgaaggc gctggacggc
    17761 gtttcgttta accttgaacg tggcaaaacg ctggcagtag tgggcgaatc tggctgcggt
    17821 aaatcgaccc tcggtcggtt gctgacgatg atcgaaacgc ccaccggcgg cgagctgtat
    17881 tatcaggggc aggatctgct caagcacgat ccgcaggcgc agaagctgcg tcggcagaaa
    17941 atccagatcg tcttccagaa tccctacggt tcgctgaatc cgcgtaagaa agttgggcaa
    18001 attctcgaag agccgctgct tattaacacc agcttaagca aagaacagcg tcgggaaaaa
    18061 gccctgtcga tgatggcaaa agtcggcctg aaaaccgagc actatgaccg ctatccgcac
    18121 atgttttccg gcggacaacg tcagcgtatc gctattgccc gtggtctgat gctcgacccg
    18181 gatgtggtga ttgccgatga gccggtttct gcgctggacg tctcagtgcg tgcgcaggtg
    18241 ctgaatctga tgatggattt gcagcaggag ttggggcttt cttatgtctt tatctcccac
    18301 gacctgtcgg ttgtggagca cattgctgat gaagtgatgg tgatgtacct aggccgctgc
    18361 gtggagaaag gaacgaagga ccaaatcttc aataacccgc gccatccgta cacccaggcg
    18421 ctgctctccg cgacgccacg cctgaacccg gacgatcgtc gtgaacgcat caaactcacc
    18481 ggtgaactgc caagcccgct gaatcctccg ccgggttgcg ccttcaacgc ccgctgtcgt
    18541 cggcgcttcg gcccctgcac ccagttgcag ccgcagctaa aagactacgg aggtcaactg
    18601 gtagcttgtt ttgctgttga tcaggatgaa aatccgcagc gttaactcac aaaaccgggg
    18661 ccatgcgctc cggtttactc aatcagcttc aacagtggcg acaagcatag caaaatgccg
    18721 taaagcagaa tcagccaggc tttaaatccg cgaagttttt ccagttgtgc gactttatag
    18781 atcaggaaaa acggaatgag gcacgacaca atgccatata acgggcttcc caactgaaag
    18841 aacaccagca ctgatacacg aaacgaaacc caaatcgtca acgtaatgac gataaaagcg
    18901 cagatcgcca gagtcagcat gcgtgagtta attttcttgg tatcaataat tcggcttaac
    18961 agattgagaa taatgccttt aatagcctcg tggaaaccga gataaatgcc aaagaatgcg
    19021 gtcagtacgg caaagatatt cagcaccgta gaggtgatat gaatgatatg tccagggatc
    19081 acctgcgcgg ccagtgccag tgccgagata ttttgttcaa aggcagaaac ggcttcttcg
    19141 tggctaattg agaaggtaaa cgaaaaggca aaaaacagga tcaccgcgat gagcgtgata
    19201 taactaattc ggtgggtacg cagcgcgagc cgggttgcca gtactttatc tgcttcccgt
    19261 ttacgatagg caatattcat tgggttaagt acctgaataa ataccgcaga aaagaagcaa
    19321 aatggaatgg taagcaggac atcgcggaaa aagacggagg cttgcgggaa ggcggttata
    19381 ttggcgaaat tccagtgcgg gatcatcgca aaaccgaaca cgacaataat ccctactttg
    19441 accaccacca ttggcccaga aatcttaaac agtaatcgtt caccaccaga cgcaatcgct
    19501 atcagtacgg cgaaaatagc gactttataa agtagagatt gtgaaagatc ggcatcggtt
    19561 aaaccgaagg ttttcaggta cgaggcgctg tcgaaaacca cggagagaga gtagataaaa
    19621 atcccgtgga taatcatcaa aaagtagata accccgagga aaattcccca gttcttcccc
    19681 aggtaatgac tgataatatc ggtgtagtca ttacaggaat cgctttcaga aagggttttt
    19741 aaataaatgt cctgcactac ccaggtgaca ggataagcaa tgatcgctgc ggtaataaat
    19801 acccaaattc ccttcaagcc aatttgtact ggcatcagca cggttccggc accaatcgcc
    19861 atgcctatgc ataataaaac ccagccgaag tcgtagcgtg taaacggcaa tttctgattg
    19921 tgtttcgata gtgtgttgtg ctgcataaag tcctgtccag aaagaagtcg gcctcgcaaa
    19981 aatgaataaa cccagtttct cttgcgcaac aggtggagaa gtcgaatacg cagtatgtaa
    20041 aaaatgaggc aacagattca ctggggaggt cgcgaggagg cgcaattcta caaaattgcg
    20101 catttcacta ccagtctgga gtcagcccgg atatgaactg caactattgc aaataaagaa
    20161 attaagctgg aaggcatcgc gcaatgagga agattgccgg gtgctgacag taaaaagca
//
LOCUS       DAJTSA010000076        19698 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000076 DAJTSA010000000
VERSION     DAJTSA010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.076"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO369_002841"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO369_002841"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR5983052.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO369_002842"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO369_002842"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR5983053.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVLEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO369_002843"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO369_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR5983054.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="OO369_002844"
     CDS             6038..6658
                     /locus_tag="OO369_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR5983055.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="OO369_002845"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="OO369_002845"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR5983056.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="OO369_002846"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="OO369_002846"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5983057.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="OO369_002847"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="OO369_002847"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR5983058.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="OO369_002848"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="OO369_002848"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR5983059.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="OO369_002849"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="OO369_002849"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            11276..12119
                     /gene="ttdA"
                     /locus_tag="OO369_002850"
                     /pseudo
     CDS             11276..12119
                     /gene="ttdA"
                     /locus_tag="OO369_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12116..12721
                     /gene="ttdB"
                     /locus_tag="OO369_002851"
     CDS             12116..12721
                     /gene="ttdB"
                     /locus_tag="OO369_002851"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR5983060.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12769..14232
                     /gene="ttdT"
                     /locus_tag="OO369_002852"
     CDS             12769..14232
                     /gene="ttdT"
                     /locus_tag="OO369_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR5983061.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="OO369_002853"
     CDS             complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="OO369_002853"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR5983062.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15526..15741
                     /gene="rpsU"
                     /locus_tag="OO369_002854"
     CDS             15526..15741
                     /gene="rpsU"
                     /locus_tag="OO369_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR5983063.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15852..17597
                     /gene="dnaG"
                     /locus_tag="OO369_002855"
     CDS             15852..17597
                     /gene="dnaG"
                     /locus_tag="OO369_002855"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5983064.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17792..19633
                     /gene="rpoD"
                     /locus_tag="OO369_002856"
     CDS             17792..19633
                     /gene="rpoD"
                     /locus_tag="OO369_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR5983065.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4684 a         5506 c         5030 g         4478 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca agatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccagcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcatcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccgctg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct ataccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtgtcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattaa cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac aggcagtgaa taagttgaca gagattgtcg ctaactttac
    11341 cgccatgatt tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga
    11401 tgccgaaacg tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa
    11461 agcgattgac ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa
    11521 agtcggttcc cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga
    11581 agaggcaacc gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa
    11641 caccggcaaa aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa
    11701 tgacgatgcg gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc
    11761 gaaagtgtta atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat
    11821 ctccaccctc gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc
    11881 ggtggaaacc gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca
    11941 tcccaatcca aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg
    12001 gattggtcca caagggctga ccggcaacag ttcagtgatg ggcgcatcgt cgcggcacgc
    12061 tgctggttca tgccgatctc acctttgaaa atctgtctca cacccggagc gcgttatgaa
    12121 aaagatcctg acaaccccga tcaaagctga agatctgcaa gatattcgcg tcggcgatgt
    12181 gatctacctg accggtacgc tggtgacctg ccgcgacgtt tgtcaccgcc gtttgatcga
    12241 actgaaacgt ccgatccctt acgatctcaa cggcaaagcg attttccacg ctggccccat
    12301 cgtgcgcaaa aacggcgaca aatgggagat ggtctccgtc ggcccgacaa ccagtatgcg
    12361 tatggaaagt tttgaacgtg aatttattga gcagaccggc gtaaaactgg tggttggcaa
    12421 aggtggtatg gggccgctga ccgaagaagg ctgccagaaa ttcaaggcgt tacatgtgat
    12481 tttcccagca ggctgcgcgg tgctggcggc aacccaggta gaagagattg aagaagtgca
    12541 ctggacagaa ctcgggatgc cggagtcact gtgggtctgc cgggtcaaag agttcggccc
    12601 gctgattgtc tctattgata cccacggcaa caacctgata gccgaaaaca aaaagctgtt
    12661 cgccgaacgc cgcgatccca tcgtggaaga gatctgcgag cacgtccatt acatcaaata
    12721 accctcccgg agaggctcac ccctctcctt tttcgcaggc ataacacgat gaaaccttcc
    12781 actgaatggt ggcgatatct tgcgccgctg gcggtcatcg ccattattgc tctacttccg
    12841 gttcccgcag ggctggagaa ccatacctgg ctctactttg ccgtttttac tggcgtgatc
    12901 gttggactga tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatctccatc
    12961 atcgccatac tctctccctg gctgctgttc agccccgaac agctcgccca gccaggcttt
    13021 aagttcaccg ccaaatccct ctcctgggca gtttctggtt tttctaattc ggttatctgg
    13081 ctcattttcg ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc
    13141 attgcgctga ttctggtgaa aaagatgggg catcgcacgc tgtttctcgg ctatgcggtg
    13201 atgttctccg agctgatcct ggcacctgta acaccgtcca actcggcgcg tggtgcgggt
    13261 attatctatc ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc
    13321 agttcgcgca gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc
    13381 agcgccattt tcctgacggc gatggcacct aacttgctgt taattggact gatgaaaagc
    13441 gcatctcacg ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagcatt
    13501 ttactggttc tgctggttcc ctggctggct tacgtgctgt acccgccggt actgaagtct
    13561 ggtgatcagg tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg
    13621 cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg tgctgtggat tttcggcggt
    13681 gattatatcg atgccgcgat ggtcggttac agcgtggtgg cgctgatgct gcttctgcgc
    13741 attatcagtt gggacgacat tgtcagtaat aaagcggcat ggaacgtttt cttctggctg
    13801 gcctcgctta tcaccctcgc gaccggactc aacaataccg gttttattag ctggtttggc
    13861 aaactgttag caggcagctt aagtggttat tcgccgacga tggtgatggt gacgttgatt
    13921 gtggtgtttt atctactgcg ctactttttc gccagcgcca cggcgtatac ctgcgccctc
    13981 gcaccgatga tgatcgccgc cgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc
    14041 ctgatggttg gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc
    14101 agtccgattt actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg
    14161 atttttgggc tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg
    14221 gtgttgcttt aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc
    14281 agccggtaac tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc
    14341 gcctgcttta aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa
    14401 ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc atcatttcag ccagcttcgc
    14461 ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg
    14521 atccaacgct cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg
    14581 ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa
    14641 ggttttcaga ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa
    14701 aacaaagcgc ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata
    14761 atccagcccc agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga
    14821 ctcgccgagc agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc
    14881 ggacaccagc agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa
    14941 cagatgccct tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga
    15001 acgccccacg gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc
    15061 cacagcatca atgtcttttg ccgttaaacc agactccttt agcgccgcct ggatcaacgg
    15121 tacggtttta cgcacatgat cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc
    15181 gtgcaatttc acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat
    15241 ggcgatgccg gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac
    15301 ctcgctttat taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc
    15361 ctggagaaag cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaaggccgc
    15421 ggtgctttac aaagcagcag caattgcagt aaaattccgc accattttga aataagctgg
    15481 cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa
    15541 gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa
    15601 gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt
    15661 aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca
    15721 cgccgcactc gtctgtacta attccccgag agcgttgctc tccgatcaga ccgagttgta
    15781 gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa
    15841 aatcggggcc tatggctgga cgaatcccac gcgtattcat taatgatctg ctggcacgca
    15901 ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc
    15961 acgcgtgttg tccattccac aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac
    16021 agttttacca ctgctttgga tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact
    16081 acgacaagct cgagttcgtc gaaacggtcg aagagctggc agcaatgcac aatcttgaag
    16141 tgccatttga agcaggcagc ggccccagcc agatcgagcg ccatcagagg caaacgcttt
    16201 atcagttgat ggacggtctg aatacgtttt accaacaatc tttacaacaa cctgttgcca
    16261 cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca cgaggttatc gctcgctttg
    16321 cgattggttt tgcgcccccc ggctgggaca acgtcctgaa gcggtttggc ggcaatccag
    16381 aaaatcgcca gtcattgatt gatgcgggga tgttggtcac taacgatcag ggacgcagtt
    16441 acgatcgttt ccgcgaacgg gtgatgttcc ccattcgcga taaacgcggt cgggtgattg
    16501 gttttggcgg gcgcgtgctg ggcaacgata cccccaaata cctgaactcg ccggaaacag
    16561 acattttcca taaaggccgc cagctttacg gtctttatga agcgcagcag gataacgctg
    16621 aacccaatcg tctgcttgtg gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg
    16681 gcattaatta cgccgttgcg tcgttaggta cgtcaaccac cgccgatcac atacaactgt
    16741 tgttccgcgc gaccaacaat gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg
    16801 ccgcatggcg agcgctggaa acggcgctgc cttacatgac agacggccgt cagctacgct
    16861 ttatgttttt gccggatggc gaagaccctg acacgctggt acgaaaagaa ggtaaagaag
    16921 cgtttgaagc gcggatggag caggcgatgc cactctccgc atttctgttt aacagtctga
    16981 tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg tttgagtacg ctggcactgc
    17041 cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca
    17101 acaaattagg catacttgat gacagccagc ttgaacgatt aatgccaaaa gcggcagaga
    17161 gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac catgcgtata cttatagggt
    17221 tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa
    17281 ataagctccc tggacttggc ttattcagag aactggtcaa cacttgtctc tcccagccag
    17341 gtctgaccac cgggcaactt ttagagcact atcgtggtac aaataatgct gccacccttg
    17401 aaaaactgtc gatgtgggac gatatagcag ataagaatat tgctgagcaa accttcaccg
    17461 actcactcaa ccatatgttt gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc
    17521 gtgagcgcac gcatggttta agcaacgaag aacgcctgga gctctggaca ttaaaccagg
    17581 agctggcgaa aaagtgattt aacggcttaa gtgccgaaga gcgatcggga agcccccgac
    17641 agccgcactg agaggcagcg gcaaatatat aagtacgccc tcgtaattat cgttggcggt
    17701 aaacaaccgt tggatttcag cgttaacggc tgaaggacat cgggtcaatc gcccaacacc
    17761 aacctcatga aataagtgtg gataccgtct tatggagcaa aacccgcagt cacagctgaa
    17821 acttcttgtc acccgtggta aggagcaagg ctatctgacc tatgccgagg tcaatgacca
    17881 tctgccggaa gatatcgtcg attcagatca gatcgaagac atcatccaaa tgatcaacga
    17941 catgggcatt caggtgatgg aagaagcacc ggatgccgat gatctgatgc tggctgaaaa
    18001 caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga
    18061 aatcgggcgc acgactgacc cggtacgcat gtacatgcgt gaaatgggca ccgttgaact
    18121 gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt gaagacggga tcaaccaggt
    18181 tcaatgctcc gttgctgaat atccggaagc gatcacctat ctgctggaac agtacgatcg
    18241 tgttgaagca gaagaagcgc gtctgtccga tctgatcacc ggctttgttg acccgaacgc
    18301 agaagaagat ctggcaccta ccgccactca cgtcggttct gagctttccc aggaagatct
    18361 ggacgatgac gaagatgaag acgaagaaga tggcgatgac gacagcgccg atgatgacaa
    18421 cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt
    18481 aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct gccgctcagg aagagatcct
    18541 gaaactgtct gaagtattca aacagttccg cctggtgccg aagcagtttg actacctggt
    18601 caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct
    18661 ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt accctgttta ccggcaacga
    18721 aaccagcgat acctggttca acgcggcaat tgcgatgaac aagccgtggt cggaaaaact
    18781 gcacgatgtc tctgaagaag tgcaccgcgc cctgcaaaaa ctgcagcaga ttgaagaaga
    18841 aaccggcctg accatcgagc aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc
    18901 gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac ttacgtctgg ttatttctat
    18961 cgccaagaaa tacaccaacc gtggcttgca gttccttgac ctgattcagg aaggtaacat
    19021 cggcctgatg aaagcggttg ataaattcga ataccgccgt ggttacaagt tctccaccta
    19081 cgcaacctgg tggatccgtc aggcgatcac ccgttctatc gcggatcagg cgcgcaccat
    19141 ccgtattccg gtgcatatga ttgagactat caacaaactc aaccgtattt ctcgccagat
    19201 gctgcaagag atgggccgtg aaccgacgcc ggaagaactg gctgaacgta tgctgatgcc
    19261 ggaagacaag atccgcaaag tgctgaagat cgccaaagag ccaatctcca tggaaacgcc
    19321 gatcggtgat gatgaagatt cgcatctggg ggatttcatc gaggatacca ccctcgagct
    19381 gccgctggat tctgcgacca ccgaaagcct gcgtgcggca acgcacgacg tgctggctgg
    19441 cctgaccgcg cgtgaagcaa aagttctgcg tatgcgtttc ggtatcgata tgaacaccga
    19501 ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc cgcgaacgta tccgtcagat
    19561 cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt tctgaagtgt tgcgtagctt
    19621 cctggacgat taatcggtag gccggatcag gcgttacgcc gcacccggca ctagtctctc
    19681 tgcacaaacg ccaccttt
//
LOCUS       DAJTSA010000077        19615 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000077 DAJTSA010000000
VERSION     DAJTSA010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19615
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.077"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            668..1459
                     /locus_tag="OO369_002857"
     CDS             668..1459
                     /locus_tag="OO369_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983066.1"
                     /translation="MTTRITFSMTSDETLRIVDEYCHTHKLSRSKVINALLSATAPVL
                     NDINCYYQLAGELQSRLLNGVYQRDLPRKRNVVSAEKYCLEIWENKLFTRRILEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRCIKDICEYQMQLSGEKTKYACFIYIERTIYNHD
                     NPSDKIPVKAAVGNAVILLAKDVIYNEYFFDLRQSFFVSVTDLMVSGAKGIPETQTYP
                     DVYCWIPLFSINSGVLITPVYKIDPLKPVTVKKPDKITVVCNYRE"
     gene            1845..2051
                     /locus_tag="OO369_002858"
     CDS             1845..2051
                     /locus_tag="OO369_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5983067.1"
                     /translation="MNTPVSLMDDQMVDMSFITQLTGLTDKWFYKLIKHGAFPPPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            2137..2709
                     /locus_tag="OO369_002859"
     CDS             2137..2709
                     /locus_tag="OO369_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983068.1"
                     /translation="MQQLENRCDLLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGSVISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(2711..2995)
                     /locus_tag="OO369_002860"
     CDS             complement(2711..2995)
                     /locus_tag="OO369_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983069.1"
                     /translation="MVVLNTPGKYQYDLRNHLLVNLFPIALDAEITTSNVNKHQLHRS
                     NIPAALSGNSGITDYVPFSAERNAAMVIWRTAIDSRPAVCNDEEGTPGTL"
     gene            2966..3109
                     /locus_tag="OO369_002861"
                     /pseudo
     CDS             2966..3109
                     /locus_tag="OO369_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            4278..5297
                     /locus_tag="OO369_002862"
     CDS             4278..5297
                     /locus_tag="OO369_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5983070.1"
                     /translation="MNNEQMLIKCRDPRKCRFYAEYLNHKYPNEFPHFAPDNPQNCIR
                     NVNLFLKEKYCNVSPPPFGTFGTTEEEALNNEINELEIIAGQAILPEESFLWLKTDEL
                     ATFFTWTTIYLSKEDHTKFPKINHNSSGIKNGIEVRKIRTVTDETLNTYKKSNLPDNP
                     SSHNERIKVIISYFDSCHSLINKNAKSIYLEELRQAWLKKKSACQKFKWLLPDDDEMC
                     QWFWTRLKQKQDEKHPVHNPVTRWFIPSSTKEYYLASLVAFALWKEAPDTIELFRTRI
                     NHAWHQKKQKDKYRSDGKKAINIHIRSNVKEMLDELRTVYGLRTGEFFEMLITEKYLE
                     HKRRK"
     gene            5407..6279
                     /locus_tag="OO369_002863"
     CDS             5407..6279
                     /locus_tag="OO369_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR5983071.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            6613..9735
                     /gene="ag43"
                     /locus_tag="OO369_002864"
     CDS             6613..9735
                     /gene="ag43"
                     /locus_tag="OO369_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCR5983072.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARAQGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGALTGNGSVEKSGSGTLTVSNTTL
                     TQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFIQGNKL
                     QAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVSG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGVY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLWADIVAQGTRHSMKASS
                     DNNDFRVRGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNASYVKFGHG
                     SAQHVRAGFRLGSHHDMNFGKGTSSRDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQASYAHSINGSSAEGY
                     NSQATLNVTF"
     gene            9856..12372
                     /locus_tag="OO369_002865"
     CDS             9856..12372
                     /locus_tag="OO369_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983073.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRTIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAISQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPAPEWYCSSLCHETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLTVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLALVAVTTSPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGKMVNRLEEAFPPELPA"
     gene            12448..12903
                     /locus_tag="OO369_002866"
     CDS             12448..12903
                     /locus_tag="OO369_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983074.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNGEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            complement(13022..13233)
                     /locus_tag="OO369_002867"
                     /pseudo
     CDS             complement(13022..13233)
                     /locus_tag="OO369_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13314..14132
                     /locus_tag="OO369_002868"
     CDS             13314..14132
                     /locus_tag="OO369_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR5983075.1"
                     /translation="MQLVSRFGYANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     TYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            14474..14947
                     /locus_tag="OO369_002869"
     CDS             14474..14947
                     /locus_tag="OO369_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR5983076.1"
                     /translation="MKTLSQNTTSSACAPETDLQQLVATLVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            14963..15439
                     /locus_tag="OO369_002870"
     CDS             14963..15439
                     /locus_tag="OO369_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR5983077.1"
                     /translation="MQQLSFLPGEMTTRERSLILRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVPPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            15526..15747
                     /locus_tag="OO369_002871"
     CDS             15526..15747
                     /locus_tag="OO369_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR5983078.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            15827..16204
                     /gene="cbtA"
                     /locus_tag="OO369_002872"
     CDS             15827..16204
                     /gene="cbtA"
                     /locus_tag="OO369_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HCR5983079.1"
                     /translation="MSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAAPATTV"
     gene            16251..16628
                     /locus_tag="OO369_002873"
     CDS             16251..16628
                     /locus_tag="OO369_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR5983080.1"
                     /translation="MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKQ"
     gene            16625..17113
                     /locus_tag="OO369_002874"
     CDS             16625..17113
                     /locus_tag="OO369_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983081.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLSELINKVSENGYS
                     LRVVDKSDQHATSTPPPLTTLTCIRCSTAHITETDNAWLYSLSHQTNDDGETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDAGAECLPGFPTF
                     DW"
     gene            17133..17330
                     /locus_tag="OO369_002875"
     CDS             17133..17330
                     /locus_tag="OO369_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983082.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            17415..18257
                     /locus_tag="OO369_002876"
     CDS             17415..18257
                     /locus_tag="OO369_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCR5983083.1"
                     /translation="MSEITASRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEVSAMTESIRGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDRELWRDLMQRS
                     GMLSLMDAQTRDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFKGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
     gene            19034..19279
                     /locus_tag="OO369_002877"
     CDS             19034..19279
                     /locus_tag="OO369_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983084.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
BASE COUNT         5247 a         4945 c         4805 g         4618 t
ORIGIN      
        1 actcttgact ataaagatga tgacgggatc agaacagtta ttaatccata caacaaaata
       61 cagaaaacaa cagaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aataacagac
      121 attacttaac aaagaacatt acagtcagtg tgtacattac agatttatat tcaggtgttg
      181 cgggactaca ttacagcaga gaggggtaag gacagagtta caagtactac tagagagaat
      241 atcactgcac agatacttct atgagtatat ctttatgaga gatccgtcta caggattggg
      301 ttataaggtg tatttacaag gtataatcac aggatcattt acatgaccat acttctgtaa
      361 ataatactaa ttaaccttaa ttattttttt atggtacatg aactacctgt agctgtaacg
      421 ataaacaaat cacccaaaat gaaaaatact ggttatcaca gttcagagaa ctcgtatgag
      481 tgtccccctg agcaatgctt cattttctgt gattctgtgt gatagagtta tactgagtac
      541 agtggcagta acactgcttt ccatcacttg aggataaaat tcattaaaaa attaattgca
      601 tgaattaaca ggttttttta tgaaatgtta tgcatctcaa ccaatatgat taacagaaaa
      661 cttcatcatg acaacacgta taacgttcag tatgaccagt gatgagacgc tgcgaattgt
      721 ggatgagtac tgtcataccc acaaactttc gcgctctaag gtaatcaacg cattactcag
      781 cgccacagca cctgtcctta atgatattaa ctgttattat caacttgcag gcgaactgca
      841 gtcacgctta ttaaatggag tgtatcaacg ggatcttcct cgcaaacgca atgtcgtcag
      901 tgcagagaag tattgtctgg aaatctggga aaacaaactt ttcaccagaa gaatcctgga
      961 gtttgacagt tcaaatggtg ttctgtacgc gctgaaacat aaaagacact atcgccggga
     1021 taagatgatt ggaagggttg aaagtcggtg tatcaaagac atttgcgaat accagatgca
     1081 gctatccggg gagaagacga aatatgcctg ttttatttat atcgaaagaa caatatacaa
     1141 tcacgataat ccatctgata aaatacctgt aaaagcggct gttggtaatg cagtcatcct
     1201 gctggcaaag gatgtgatat ataacgagta cttttttgat ttgcggcagt cattttttgt
     1261 gagcgttaca gatctgatgg tctctggcgc aaaaggtatt ccggagacac agacatatcc
     1321 ggacgtttat tgctggatcc ctcttttttc cataaattcc ggcgtgctca ttacccctgt
     1381 atataaaatt gaccctctga aacccgtaac ggtaaaaaaa ccggataaga tcactgttgt
     1441 ttgtaattac agagaataat ttcagccttc taccttcacc gcgacagtgt ttacctctgc
     1501 tttatcttcc gttcagtaaa tacaaaccac cgctctggca ggtacagggc gtggtttatc
     1561 tccgatactc ccctgtgcag taataatcat cactgaaaat actgatcccg tcacggcaaa
     1621 acgccatccc atattccggc aacacaaaat catcttccgt ttataccgga ttcactgaca
     1681 aaacagacaa cctgtattgc ctgtacacaa cagtttctct cctggcagga atattcccct
     1741 actcattttt tacgtttctc cccggttgtt atgccggtga aggtggtgcg tcgttttcat
     1801 caccaacgcc ggttgagatt aacaacatcc ggaggaacat tctcatgaat accccagttt
     1861 cactgatgga tgaccagatg gtcgacatgt cgtttatcac tcagctgacc ggcctgaccg
     1921 ataagtggtt ttataaactc atcaagcatg gagcctttcc gccccccatc aaactgggcc
     1981 gtagctcccg ctggctgaaa agcgaagtgg aagcctggct gcaggcgcgt attgcacagt
     2041 cccgtccgta attcctgacc gttatccgtt ccaccgcagc aaaggcatga tccggccccc
     2101 ggaggcattt tgctgcgcgc taaaggagag aatattatgc aacaacttga aaacaggtgt
     2161 gacctgctgc tcatccagca tcagaaatgg atgacaagcg tgacgaggct tatcgtcgct
     2221 catggaatgg ggtctccgca tcttcatggt tatcaccgtc tgaccctggc acactttttt
     2281 ctgccggaaa aaggctccgt catttcggtg gccccccagg gactctatca ggtcgtcaac
     2341 ccgggaacac caccgttcat tcctgccatt caggaagggt taatgaccag cattcagacc
     2401 catgaaatca tgctgcttac acattttaat ctgggcggcg ttttactcag tgagctacat
     2461 cgtctgggtg aaaaccggct ggcaaacagg ctgaacagcc tgctccggcg tttcgacgat
     2521 cgtgatctct accacacgct gatatggctg tgctggtatg atctgatgtg tgcccactcc
     2581 atgcagccct ggactgaaga gttaaagcat aaatcacatg cagagcttga aaactgggct
     2641 gtggcgcgca aacgggaaaa gcgcgagctg gaactgatga ttgacgagta cctgttatac
     2701 gcctgctgat tcataacgtt cccggggttc cttcctcatc gttacacaca gccggacggc
     2761 tatctatggc tgtccgccaa atgaccatcg ccgcatttcg ttccgctgaa aacggtacgt
     2821 agtcagtgat accggaattt ccggagaggg ccgcaggtat attgcttctg tgcaattgat
     2881 gcttatttac attggatgta gttatttctg catccaatgc tatcggaaag agattaacca
     2941 gcaaatggtt cctcaaatcg tattgatatt tacccggcgt attgagcacc accatctgac
     3001 actccgttcc cgcatcaaac gactggaccg aaaaacgatt tacttttcac gtgctgtgga
     3061 aattcacgaa aaaattattg ggtcattcat cgaaaaacat atgttctgat gactggagtc
     3121 atcctccaga aatatacgtc aactgaacaa tatcgatttt ttatgggatt ctatacactt
     3181 ttccatatcc ctctgaaaat cagtcatcag ctcaccaaca cctcttctga aacttgtatt
     3241 aaacttattg cttaaataga tgatataaca tctcttccat tgcactgaat cggcgaaaac
     3301 tgccgtttca ataatctcaa caatattatt tatcggctaa ctttatcagt aaacaatgga
     3361 atgtcacact gatgaaatga acattcagag taagatgata aaattcatat aaacgccatt
     3421 gctgaatatt atttatttca tacagataaa cgataaaata caaaagggta tatttctcaa
     3481 tgtaacagtc aattattcat ctccactggc gagcacggtc agcacacaaa caaagttagt
     3541 cctcctgctt aatcattgcg tatcagaaac taaaaaagaa aaaattctgc agacaactta
     3601 acaatcttca tcacatttcc tgaagacaga catatccaca aacggtagtc agcgttgaat
     3661 taacaagtct tacagagcta tacgtagctt ataaagccac tcaatgttca tcatcacccg
     3721 actacttatc tggaatacca ccagtataaa ctcatcaata aaaacacctg ttgttaaaat
     3781 aagaatactg tcagcagtaa attattaaaa caacatctgt ccacactatc tgttctttta
     3841 acgccaacgg cagcgaaaat agtgcatcac agccttcttt tttctttcgc caccactcag
     3901 acaaacacaa cactcacacc cacaccacaa aacagaatca aaacataaaa ttaacaataa
     3961 cagtcaaaag gaaaacacag aatattgagg tggactgtca ccaggacagc gcatacgacg
     4021 catgtgatca gcacatcccg ttcttcgctg gcatccccaa aatgcagaac aaacaatcat
     4081 aacattatga atataaaaga aaatatgatt tttcaccatg aaaaaaacat caatgtatct
     4141 ctgtataaaa ataatgataa catgacataa acatttaaaa acataaataa acagcaacaa
     4201 aaacatttga taatcactta gtgattaaat acaattaaga cgattttgtt ataagagagg
     4261 caccatggag tgagataatg aataacgaac agatgcttat caaatgcaga gatccccgta
     4321 aatgtcggtt ttatgccgaa tatctgaatc acaaataccc taatgaattc cctcactttg
     4381 cgcctgacaa cccacaaaat tgtatcagga atgttaattt atttttaaag gaaaagtatt
     4441 gtaatgtttc acctcccccc tttggtactt ttggtactac tgaagaagaa gcactcaata
     4501 acgaaattaa tgagcttgaa attattgccg ggcaggcaat actgcctgaa gagagttttc
     4561 tctggttgaa aactgacgaa cttgcgactt tctttacctg gactactatt tatctgtcga
     4621 aggaagacca tacaaaattc ccgaaaataa accataattc atcaggaata aaaaacggaa
     4681 ttgaagtcag gaaaatacga acagttaccg atgaaacgtt aaacacttac aaaaaaagta
     4741 accttccgga taatccatca tcccataacg aacgaataaa agtaatcatt tcatattttg
     4801 actcctgcca ttctttaatt aataaaaatg caaaaagcat atacctcgag gaactcaggc
     4861 aggcgtggct taaaaagaaa agcgcttgtc aaaagtttaa gtggctcctt cctgacgatg
     4921 atgaaatgtg tcagtggttc tggacaagac tgaaacaaaa acaggatgaa aaacatccag
     4981 tacataaccc agtgacccgg tggttcatac catcttcaac gaaagaatat tatcttgcct
     5041 ctcttgtcgc ctttgcttta tggaaagaag cgcctgatac cattgaactg ttcagaacac
     5101 gaataaatca tgcctggcat cagaaaaaac aaaaggacaa atatcggagt gatggtaaaa
     5161 aggccattaa tatacatata agaagcaatg tgaaagaaat gcttgatgag ctcagaactg
     5221 tttacggtct gagaacagga gagttttttg aaatgctgat tacagagaaa tatcttgaac
     5281 acaagcgaag gaagtaattc attacctgtt acattctaaa taatctcaga cagatattat
     5341 ctccgtgaat cccccgccac ctttccggtg cgcggggttt tgtctttttt caccgggaat
     5401 acatgtatga atccgtctga tgccattgag gcaattgaaa aaccgctctc ctccctgcct
     5461 tactcacttt cccgtcacat cctggaacat ctgcgcaaac tcaccagtca cgaacccgtg
     5521 attggcatta tgggtaaaag cggggccggt aaatcttcac tctgtaatgc actgtttcag
     5581 ggggaggtca caccggtcag tgatgttcac gccggcaccc gggaagtgca gcgcttccgt
     5641 ctgagtggtc acggtcacag catggttatc actgacctgc ccggggtggg cgagagccgg
     5701 gacagggatg cagagtatga agccctgtac cgtgacattc tgcctgaact ggacctggtg
     5761 ctgtggctga ttaaagccga tgaccgggcc ctgtctgtgg atgagtattt ctggcgacac
     5821 atcctgcagt gcggacatca gcaggtgctg tttgtggtga cgcaggctga caaaacggaa
     5881 ccctgccatg aatgggatat ggcaggtatt cagccttctc ccgcacagga acagaacatt
     5941 cgtgagaaaa cggaggcggt attccgtctg ttccggcccg tacatccggt tgttgccgta
     6001 tcggcccgca ccggctggga actggatacg ctggtcagtg cgctcatgac agcgcttccc
     6061 gaccatgccg ccagccccct gatgacccga ctgcaggacg agctgcgcac ggagtctgtc
     6121 cgctctcagg cccgtgaaca gtttaccggt gcggtggacc ggatatttga cacagcggag
     6181 agcgtctgtg ttgcctctgt tgtccgtacg gccctgcgcg ctgttcgtga caccgtggtc
     6241 tctgttgccc gcgcggtatg gaactggatc ttcttctgaa cctgttgtgg atgatgtcct
     6301 ccctgcctct gagtctgctc acaaaagcgc tgttttcgtt actgtctctc ttgtccgtgc
     6361 aatagactga taatagaata aaacgatcga tacctatttt accgatcgtt aatatcgatc
     6421 gttatgctaa taataactcc tgttagcaac gtgcgcagat acacacagac atgagattca
     6481 gggaacaaca gagccacacg tcagaaactt ccgtcagccg gacctccggc actgtaaccc
     6541 tttacctgcc ggtatccaca tctgtggata ccggcttttt tattcaccct cactctgatt
     6601 aaggaaatgc tgatgaaacg acatctgaat acctgctaca ggctggtatg gaatcacatt
     6661 acgggcgctt tcgtggttgc ctccgaactg gcccgcgcac agggtaaacg tggcggtgtg
     6721 gcggttgcac tgtctcttgc cgcggtcacg tcactcccgg tgctggctgc tgacatcgtt
     6781 gtgcacccgg gtgaaacagt gaatggcgga acactggtaa accatgacaa ccagtttgta
     6841 tccggaacag ctgatggcgt gactgtcagt accgggcttg agctggggcc ggacagtgac
     6901 gaaaacaccg gcgggcaatg gataaaagcg ggtggcacag gcagaaacac cactgtcacc
     6961 gcaaatggtc gtcagattgt gcaggcagga ggaactgcca gtgatacggt tattcgtgat
     7021 ggcggagggc agagccttaa cggactggcg gtgaacacca cgctggataa cagaggtgag
     7081 cagtgggtac acgggggagg gaaagctgcc ggtacaatta ttaaccagga tggttaccag
     7141 accataaaac atggcggact ggcaaccgga accatcgtca acaccggtgc agaaggtggt
     7201 ccggagtctg aaaatgtgtc cagcggtcag atggtcggag ggacggctga atccaccacc
     7261 atcaataaaa atggccggca ggttatctgg tcttcgggga tggcacggga caccctcatt
     7321 tacgccggtg gtgaccagac ggtacacgga gaggcacata acacccgact ggaggggggt
     7381 aaccagtatg tacacaacgg tggcacggca acagagacgc tgataaaccg tgatggctgg
     7441 caggtgatta aggaaggagg aactgccgcg cataccacca tcaaccagaa aggaaagctg
     7501 caggtgaatg ccggcggtaa agcgtctgat gtcacccaga acacgggcgg tgcactggtt
     7561 accagtacag ctgcaaccgt caccggcaca aaccgcctgg gagcattctc tgttgtggag
     7621 ggtaaagctg ataatgtcgt actggaaaat ggcggacgcc tggatgtgct gaccggacac
     7681 acagccacta atacccgcgt ggatgatggc ggaacgctgg atgtccgcaa cggtggcacc
     7741 gccaccaccg tatccatggg aaatggcggt gtactgctgg ccgattccgg tgccgctgtc
     7801 agtggtaccc ggagcgacgg aaaggcattc agtatcggag gcggtcaggc ggatgccctg
     7861 atgctggaaa aaggcagttc attcacgctg aacgccggtg atacggccac ggataccacg
     7921 gtaaatggcg gactgttcac cgccaggggc ggcacactgg cgggcaccac cacgctgaat
     7981 aacggcgcca tacttaccct ttccgggaag acggtgaaca acgataccct gaccatccgt
     8041 gaaggcgatg cactcctgca gggaggcgct ctcaccggta acggcagcgt ggaaaaatca
     8101 ggaagtggca cactcactgt cagcaacacc acactcaccc agaaagccgt caacctgaat
     8161 gaaggcacgc tgacgctgaa cgacagtacc gtcaccacgg atgtcattgc tcagcgcggt
     8221 acagccctga agctgaccgg cagcactgtg ctgaacggtg ccattgaccc cacgaatgtc
     8281 actctcgcct ctggtgccac ctggaatatt cccgataacg ccacggtgca gtcggtggtg
     8341 gatgacctca gccatgccgg acagattcat ttcacctcca cccgcacagg gaagttcgta
     8401 ccggcaaccc tgaaagtgaa aaacctgaac ggacagaatg gcaccatcag cctgcgtgta
     8461 cgcccggata tggcacagaa caatgctgac agactggtca ttgacggtgg cagggcaacc
     8521 ggaaaaacca tcctgaacct ggtgaacgcc ggcaacagtg cgtcggggct ggcgaccagc
     8581 ggtaagggta ttcaggtggt ggaagccatt aacggtgcca ccacggagga aggggccttt
     8641 atccagggga ataagctgca ggccggtgcc tttaactact ccctcaaccg ggacagtgat
     8701 gagagctggt atctgcgcag tgaaaatgct tatcgtgcag aagtccccct gtatgcctcc
     8761 atgctgacac aggcaatgga ctatgaccgg attctggcag gctcccgcag ccatcagacc
     8821 ggtgtaagcg gtgaaaataa cagcgtccgt ctcagcattc agggcggtca tctcggtcac
     8881 gataacaacg gcggtattgc ccgtggagcc acgccggaaa gcagcggcag ctatggcttc
     8941 gtccgtctgg agggtgacct gctcagaaca gaggttgccg gtatgtctgt gaccgcgggg
     9001 gtatatggtg ctgctggcca ttcttccgtt gatgttaagg atgatgacgg ctcccgtgcc
     9061 ggcacggtcc gggatgatgc cggcagcctg ggcggatacc tgaatctgat acacaacgcc
     9121 tccggcctgt gggctgacat tgtggcccag ggaacccgcc acagcatgaa agcctcatcg
     9181 gacaataacg acttccgcgt ccggggctgg ggctggctgg gttcgctgga aaccggtctg
     9241 cccttcagta tcactgacaa tctgatgctg gagccgcaac tgcagtacac ctggcaggga
     9301 ctctccctgg atgacggcca ggataacgcc agttatgtga agttcgggca tggcagtgca
     9361 caacatgtgc gtgccggctt ccgtctgggc agccaccacg atatgaactt tggtaaaggc
     9421 acctcatccc gtgataccct gcgcggcagt gccaaacaca gtgtgcgtga actgccggtg
     9481 aactggtggg tacagccttc tgttatccgc accttcagct cccgggggga catgagcatg
     9541 ggtacagccg cagccggcag taacatgacg ttctcaccgt cacagaatgg tacgtcactg
     9601 gacctgcagg ccggactgga agcccgtgtc cgggaaaata tcaccctggg cgttcaggcc
     9661 agttatgccc acagcatcaa cggcagcagc gctgaaggtt ataacagtca ggccacactg
     9721 aatgtaacct tctgataatt ctgcattgcc tctctgtggt cccggtcatg atgaccggga
     9781 cccggacggg ggaaaacgtt tcagtgccac attcactggc attcacaata acatgatatt
     9841 catcacggag tgactatgtt acagatagtc ggcgcgctga ttctgctgat cgcaggattt
     9901 gccattcttc gccttttgtt cagagcatta accagcacag cgtctgcgct ggcagggttc
     9961 atattgctgt gtctgttcgg tccggcttta ctggctggct atatcactga acgcataacc
    10021 cggttattcc atattcgctg gctggcaggc gtatttctga cgattgccgg aatgatcatc
    10081 agcttcatgt ggggacttga tggtaaacat atcgcactgg aggctcatac ttttgactct
    10141 gtaaaattta ttctgaccac cgctctcgcc gctggtctgc tggctcttcc cgtgcagata
    10201 agaaccattc agcagaacgg gctcacacct gaagatatca gcaaggaaat taacgggtat
    10261 tactgctgtt tttatactgc ttttttcctt atggcgtgtt ctgcatacgc accattgatc
    10321 gcattgcagt tcgatatttc accctcactg atgtggtggg gcgggttgtt gtactggctg
    10381 gctgcattag tgacgctgct atgggcggcc agccagatcc aggcgctgaa aagactgacc
    10441 agtgccatca gccagacact ggaagaacaa ccggtgctca acagtaaatc gtggctgagc
    10501 agtttgcaaa acgattacag ccttcctgaa acgctgacgg agcgcatctg gctgacactc
    10561 atttcacaac ggatttcccg gggagaactg agggaatttg aactggcaga cggaaactgg
    10621 ttactgaaca atgcctggta tgaaagaaac atggcagggt ttaacgaaca gttgaaagag
    10681 aacctgtcat tcacacctga tgaactgaaa acgctcttcc ggaaccgcct gaatttatca
    10741 ccggaagcga atgacgattt tctcgatcgt tgcctggacg gcggtgactg gtaccccttt
    10801 tcagaaggcc gccgttttgt atcattccac cacgtggatg agcttcgtat ctgtgcctcc
    10861 tgcgggctga cagaagtaca tcatgccccg gaaaatcata agccggctcc ggaatggtac
    10921 tgctcctctc tttgtcacga aacagaaaca ctgtgtcagg acatttatga acgttcttac
    10981 accggtttta tttccgatgc aacggcgaat ggtctgattc tcatgaaact gccggaaacc
    11041 tggagtacaa atgagaaaat gtttgcttcc ggagggcagg gacatgggtt tgccgctgaa
    11101 cggggaaacc atattgtcga cagagtccgt ctgaaaaacg cacggatcct cggtgataat
    11161 aatgccagaa atggagcaga cagactggtc agcggaacag aaatccagac gaaatattgt
    11221 tcaactgcag cccgtagcgt cggtgcggca ttcgacggac aaaacggaca gtatcgttac
    11281 atgggaaatc atggtcccat gcaactggaa gtcccccgtg atcagtatgc cggcgctgtg
    11341 gaaaccatga agaataagat ccgcgaaggt aaagtacccg gtgtaaccga tcccgcagaa
    11401 gcgtcccggc tgattcgtcg gggacatctg acttataccc aggcccgtaa tatcacccgg
    11461 ttcgggacca tcgaatcggt cacttatgat attgccgagg ggtcggttgt cagtctggcg
    11521 gccggaggga tcagttttgc cctgacggca tcggtcttct ggctcagcac cggcgatcgc
    11581 gatgctgccc tgcagacagc tgctgtccag gcaggaaaaa ccttcacccg cacactggct
    11641 gtctacgtca caacccagca acttcaccgg ctcactgttg ttcagggtat gctgaagcat
    11701 attgattttt cgacggccag cccgactgtc cggcaggcgc ttcagaaggg gaccggtgca
    11761 ggaaatatca gtgccctgaa caaagtgatg aagggtacgc tggtgacatc tctggcactg
    11821 gtagctgtca caaccagccc tgacatgatc aaaatgttgc ggggacggat ctccggtgcg
    11881 cagttcatca ggaatcttgc cgtggcatct tccggtgtgg caggtggtgc tgtcgggtca
    11941 gtggcgggcg ggatattgtt cagtccactg ggaccatttg gtgcactgac agggcgtgtg
    12001 gttggcggtg ttctgggggg aatgattgcc tccgctgtat caggaaaaat tgccggagcg
    12061 ctggttgaag aagatcgcgt caaaattctg gcaatgattc aggagcaggt gacatggctt
    12121 gccggcagtt tcctgctgac cggacatgag attgaaaatc tgaacgcgaa tctggcccgt
    12181 gttatcgatc agaatgctct ggagatcatt ttcgccgccg gtatacaaca acgggccgcg
    12241 accaatatgt taatcaaacc actggtggtc agtatcatca ggcaacgccc cgtcatggaa
    12301 tatgatgcat cccatctcgg caagatggtt aaccggctgg aagaagcatt ccccccggag
    12361 ctcccggcat aacgtgtgtt ctgctccttt taggttttat cacctgattt gccagtgtca
    12421 tcgtcatgac tcagagaggt aaataccatg attcacctgt tcaaaacctg catgattacc
    12481 gccttcattc tggggttaac gtggtctgcc ccactccggg cacaggatca acgttacatc
    12541 agtatacgca atacagatac gatatggctc ccgggaaata tttgtgctta ccagttccgg
    12601 ctggataatg gcggaaacgg tgaaggattt ggccccctca ccatcactct gcaactcaaa
    12661 gacaaatatg gtcagacgct ggtgaccaga aaaatggaaa cggaagcttt tggtgacagt
    12721 aatgccacgc gaaccacaga cgcatttctg gaaacggagt gcgtggaaaa tgtcgccaca
    12781 accgaaatca ttaaagcaac tgaagaaagt aacggccatc gtgtcagtct gccgttatcg
    12841 gttttcgatc cccaggacta ccatccactg ctgattaccg tttccggaaa aaatgttaac
    12901 tgatccctga agtcagtttc atgcttcagc ctctgccaca acgtatggca gaggctattt
    12961 atcaggagaa accagatatg ccgggttaca cacagagtat gtcctggctg aaggatcttt
    13021 ctcatacggg caggcggttg ctgtcataac cgcttaccgc aatgtgttta tccaggatga
    13081 cccgggaatg catttccgtc gggttatccg caacgcggaa ggtcagcgac gctgacgcag
    13141 ccggaactct gaagctgatg ccggaaaaca gcttaatgcg tggctcgcca gtggagggct
    13201 tctcagacaa taaacttcat tgatttcatc catcaggccg cgtcttctcc gggagacgcg
    13261 gcttttttca tttgttgcac cagtaaatct taaccaccga taaggagcaa agtatgcaat
    13321 tagtcagtcg ttttggttat gcgaaccaga tacgccgtga ccgtccgctg acacatgaag
    13381 agctgatgca ccatgtgccg gggattttcg gggaagagaa acacacgtcc cgaagccaga
    13441 actatacgta catccccacc atcaccgtac tggaaagtct gcagcgggaa ggcttccagc
    13501 cctttttcgc ctgccagacc cgtgtgcgcg acccgggccg ccggggatac acaaaacaca
    13561 tgctgcgtct gcggcgggcc ggagagataa acggagaaca tgtccctgaa attattctgc
    13621 tcaactccca tgacggtacc tccagctacc agatgctgcc gggttacttc agattcgtct
    13681 gccagaacgg gtgtgtctgt ggtcagtctc tgggggaagt gcgtgttccg caccggggaa
    13741 acgtggtgga gaaagttatt gaaggggctt acgaagtggt gggcgtgttt gaccggattg
    13801 aggagaaacg tgatgccatg cagtcgctgg tcctgccgcc accggcacgc caggcgctgg
    13861 cacaggcggc actgacttac cgttatggtg acgaacatca gcccgtcacc accgccgaca
    13921 ttctgacgcc acgacgccgg gaggattacg gtaaggacct gtggagtact tatcagacca
    13981 tccaggagaa tatgctgaaa ggcgggattt ccggccgcag tgcaaaagga aaacgtatcc
    14041 acacccgtgc cattcacaac atcgacaccg atattaagct caaccgcgca ttgtgggtga
    14101 tggcagaaac gctgctggag agcctgcgct gatgcagcat gtccctgaag gcgtgattca
    14161 ggcaccagaa tcacccgacg ccccggaggc gctgccgggg tgtcgttcca tttctgatga
    14221 tgacctgtgc gcctggtgta cccgtctgct ttaccgtccc ggagaaatca gtctgtgcag
    14281 actgagtata caggcaatgg catctggcca tccgtctgtg accggaacgg ttacgcgtac
    14341 ggctgtccgg aatttcagcc gaatatcatt catccctgat attaccgggt catcacggtc
    14401 agtgatgaac gtgatgttgc cagctcccga cctttactcc ctctcattta ttcacattac
    14461 aaggatttca tacatgaaaa cattgtctca gaacaccacc tcttcggcct gtgcaccgga
    14521 gactgacctg caacaactgg ttgccaccct cgtccctgat gaacagcgca tcagcttctg
    14581 gccgcagcac tttggcctca ttccacagtg ggtgaccctg gagccccgtg tcttcggctg
    14641 gatggaccgt ctgtgcgaag actactgcgg tggtatctgg aatctgtaca ccctgaacaa
    14701 cggcggggca tttatggcac ccgaaccgga tgacgatgat gacgaaacat gggtactgtt
    14761 caatgccatg aacggtaacc gcgctgaaat gagtccggaa gctgccggca ttgctgcctg
    14821 tctgatgacg tacagtcatc atgcctgtcg tacggagtgt tatgccatga cggtccatta
    14881 ttaccggctg cgggattacg ccctgcagca tccggaatgc agcgccatta tgcgcatcat
    14941 cgactgaatg gtggcaggga caatgcagca gctttccttt ctgcccggag aaatgacaac
    15001 cagagagcgc agcctcattc tgcgggccct gaaaaccctg gaccgccatc ttcatgaacc
    15061 cggcgtggcc ttcacctcca cgcatgcggc acgggaatgg ctgattctga acatggcggg
    15121 actggagcgt gaagagttcc gggtgctgta tctgaacaac cagaatcagc tgattgccgg
    15181 tgaaaccctc ttcaccggca ccatcaaccg tacggaagtc catccccggg aagtgattaa
    15241 acgcgccctg taccacaatg ccgctgccgt ggtgctggcg cacaatcacc cgtccggtga
    15301 agtccccccc agcaaggcag accgccttat cactgagcgt ctggtacagg cactggccct
    15361 ggttgatatc cgggtaccgg accatctgat agtcggtggc agccaggttt tctcctttgc
    15421 ggaacacggt ctgctttaac ccgtcaccgt cacaatcacc ttcatatcac ttcagtttct
    15481 ctttctcagc tgtttcttac tttcacattc aggaggacta ttctcatgaa aatcatcacc
    15541 cgtggtgaag ccatgcgtat tcaccgtcag catcctgcat cccgtctttt tccgttctgt
    15601 accggtaaat accgctggca cggtagcacg gatacatata ccggccgtga agtgcaggat
    15661 attcccggtg tactggctgt gtttgcagaa cgccgtaagg acagttttgg tccgtatgtc
    15721 cggctgatga gcgtcaccct gaactgagtc tggaagggaa ttctgatgag cagaattatc
    15781 gccactaccg gaccattctt aaccgatttt ctgtgaggac tttatcgtgt cagacacact
    15841 ccccgggaca acgcctcccg acgacaatca cgaccgcccc tggtgggggc tgccctgcac
    15901 cgtgacgccc tgtttcgggg cccgactggt acaggagggt aaccgcctgc attaccttgc
    15961 agaccgcgcc ggtatcagag gcaggttcag cgacgtagat gcgtaccacc tggaccaggc
    16021 ctttccgctg ctgatgaaac aactggaact catgctcact ggcggtgaac tgaatccccg
    16081 ccatcagcat accgtcacac tgtatgcgaa agggctgacc tgcgaagccg acaccctcgg
    16141 ctcctgtggt tacgtttatc tggctgttta tccgacaccg gcagcgcccg caaccaccgt
    16201 ataacaccgg atatctcccc tttataacca cctgttacag agaacttaag atgaatacat
    16261 tacccgacac tcacgtacgg gaggcatcgg gctgcccgtc tcccatcacc atctggcaga
    16321 cactgctcac ccgactgctg gaccagcact atggcctcac gctgaatgac acaccgttcg
    16381 ctgatgaacg tgtgattgag cagcatattg aggcaggaat ttcactgtgt gacgcggtga
    16441 actttctcgt tgaaaaatac gcgctggtac gtaccgacca gccgggattc agcgcaggag
    16501 ccccgtcgca gttaatcaac agcattgata ttctccgggc acgccgggca accggcctga
    16561 tgacccgcga taactacaga acggtaaata acattacccg ggggaagcat ccggaagcga
    16621 aacaatgaaa ctggccctga cgctggaagc cgacagcgtt aacgtgcagg cactgaacat
    16681 ggggcgcatt gtcgttgacg tcgatggtgt taatctcagt gaactgatta acaaggtctc
    16741 tgaaaacggt tattcactcc gcgtggtcga taaatccgac caacacgcaa ccagtacacc
    16801 accaccgtta acaaccctta cctgcatacg ttgcagcacc gcacatatca cggaaacgga
    16861 caacgcctgg ctgtactcgc tgtcacacca gactaatgac gacggcgaaa cagaatggat
    16921 tcattttaca ggcagcggct atctgttacg taccgatgca tggtcgtacc cggttctgcg
    16981 gcttaaacgc ctgggcctgt ccagaacgtt ccgtcgtctg gtcgtcactc tcacccggcg
    17041 ttatggcgtc agtctcattc atctggacgc cggcgcagaa tgtctgccgg ggttccccac
    17101 tttcgactgg taatcaggac aaaagacact ggatgaaatt attaaccaca gaaacggcgc
    17161 tggatattct gattgcgtgg ctgcaggaca atattgactg cgaatcaggg attatcttcg
    17221 acaacgatga ggataaaacg gattcggcag cactgctgcc ctgtattgaa caggccaggg
    17281 aagatatccg taccctgcgt cagctgcagt ttctgcaaca gaaccggtga gtcttactca
    17341 ccagacttca ttccactgac gccagcctga atacggctgg cgttttcgtt tatctgcaaa
    17401 aaggaatatc gattatgtct gaaatcacag cctctcgtcc ggaagtggtc aacgggcata
    17461 cggacgttat ctgctcaacc agtatccgcc acattctggc tgtacgaaag agtacattgc
    17521 tgcaaatcga cacacttatc cgacaactgg ctgaagtatc agcaatgaca gaaagtatta
    17581 gaggtaaaac ggctctggac tgggccatga aacaggattt tcgctgcggt tgctggctga
    17641 tggagaaacc ggaaaccgca atgaaagcca tcacacgcaa tcttgatcgc gaactctggc
    17701 gtgatctgat gcaacgttcc gggatgctga gtctgatgga tgcgcagacc cgggacacat
    17761 ggtacaggtc actggaatat gataattttc cggaaatcag cgaagcgaac attctgagca
    17821 catttgaaca actgcaccag aacaaagatg aagtgtttga acggggagtg atcaacgtct
    17881 tcaaagggct gagctgggat tacaaaacca actcaccctg taaatttggc agtaaaatta
    17941 tcgtcaacaa cctggtgagg tgggaccggt gggggtttca tcttatcacc gggcaacaga
    18001 ctgaccgact cgtcgacctg gaaagaatgc tacatctgtt cagcggcaaa ccgatccccg
    18061 acaaccggga aaacatcact attcgtctgg atggtcacat ccagtccgtt cagggtaaag
    18121 agcgctatga agacgagatg ttcatcatta aatactttaa gaagggatct gcacacatca
    18181 ctttcaaaag gctggaactg attgacagaa ttaacgatat aatagccagg tactttcctt
    18241 ctgtgctctc agcctgatcc cgagagtgat tccctttcga tatcaaaagg gactgcgggt
    18301 ataattgagg gtacattttt aatcaaacca aacaaattgc attaatatca atatgataaa
    18361 agaaattttc gattccgagt ccggcaccac tatttagaag aaccagccta cgggctggtt
    18421 ttttgctttc tggggttctg gtagatatcc ggtcactttg aatgctgatg tcactgcttt
    18481 acctcttcca cccagcctga acatttcttc taagtagccc ccttctggtc actgtggcct
    18541 tttctgtcaa acttcaaatc cccaatagac actatttcca catgagcacc tggctaacgg
    18601 attcattact gcacggatac atccggtctt tgtccggtct gaagtaaaac gcataccacc
    18661 tgcgttggcc cctcctcagt cccccccaac atcaaaccga acatttttca ctctgattga
    18721 tttagcttta gtcaactttt gggtgttatg cctactatat caatatgatc cataggggat
    18781 tatggccaga ctaccggtaa taccgagaga caggatctca atcggagata gaacgttctg
    18841 caaaacgggg cttacagaaa atccctaaat gacctgcaac ctccagccca ttgcatatta
    18901 tgataacagt gaatattttg gttaagtcgc gccccgcaaa aggtggaaag caatttaaca
    18961 aattgaatta aaagggttta accgcaaaat ggacgcaaca ggcaacacga cagtcacctg
    19021 cctgagtcac aaaatgaagt acaaagaagt cgcctgcggt aagcagcgga tgcactggct
    19081 ggcgctgcag ttcgtggatg agaacaacaa cccagtttcc ggtctcaacg tgcagctgga
    19141 gtatcatcca cgggcaatgg cggcagagct ggctctgtgg gccagaggca gacacactca
    19201 gtttgacccc acaccgccgc ccccacctgt cggtgtcact gacagtcagg gtctggtacg
    19261 gtttgatgac ttgtactgaa ttaaagcacc taaaacaaac atttatatcg caacagttaa
    19321 atagaatgga taatcggtaa taaaatggtc cttgagtgcg ttagatcaac atcaaaataa
    19381 ttaaaatacc catgcatttt tttaagatat ggttttagat cagaatgaga ttaaaaggga
    19441 gagaaagtca tagccttatt tgatgtcaga aaccaatgcg tggaagttag agcattacgg
    19501 aaaaaagcct gacatactta acgggcaggc atagaggtga tctcattgaa tttgttagcg
    19561 caatcagtag atcacaaaac tctatgcctg tcttcttttc tggggactcc tcagc
//
LOCUS       DAJTSA010000078        19380 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000078 DAJTSA010000000
VERSION     DAJTSA010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.078"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="OO369_002878"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="OO369_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR5983085.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="OO369_002879"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="OO369_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR5983086.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OO369_002880"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OO369_002880"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR5983087.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="OO369_002881"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="OO369_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR5983088.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="OO369_002882"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="OO369_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR5983089.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="OO369_002883"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="OO369_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR5983090.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="OO369_002884"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="OO369_002884"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR5983091.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="OO369_002885"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="OO369_002885"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR5983092.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="OO369_002886"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="OO369_002886"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR5983093.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="OO369_002887"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="OO369_002887"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR5983094.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="OO369_002888"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="OO369_002888"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR5983095.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="OO369_002889"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="OO369_002889"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR5983096.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="OO369_002890"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="OO369_002890"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR5983097.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="OO369_002891"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="OO369_002891"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR5983098.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="OO369_002892"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="OO369_002892"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR5983099.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..15634
                     /gene="atpC"
                     /locus_tag="OO369_002893"
     CDS             15215..15634
                     /gene="atpC"
                     /locus_tag="OO369_002893"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR5983100.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15985..17355
                     /gene="glmU"
                     /locus_tag="OO369_002894"
     CDS             15985..17355
                     /gene="glmU"
                     /locus_tag="OO369_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR5983101.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17517..19346
                     /gene="glmS"
                     /locus_tag="OO369_002895"
     CDS             17517..19346
                     /gene="glmS"
                     /locus_tag="OO369_002895"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR5983102.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT         4770 a         4858 c         5120 g         4632 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttattctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggca caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gagtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg ttatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggct ggcgttatca tccgatcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggag gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgccgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatacgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    15481 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    15541 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    15601 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    15661 gtctggaaac aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga
    15721 tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa
    15781 aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagat aaaaggcgtg
    15841 acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc
    15901 tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt
    15961 tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc
    16021 gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg
    16081 aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac
    16141 ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac
    16201 tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc
    16261 tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa
    16321 acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa
    16381 ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt
    16441 gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt
    16501 ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct
    16561 cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc
    16621 gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc
    16681 tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt
    16741 atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt
    16801 gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc
    16861 ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat
    16921 accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg
    16981 cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa
    17041 gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt
    17101 ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa
    17161 tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg
    17221 gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc
    17281 gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg
    17341 gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa
    17401 tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc
    17461 ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt
    17521 gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac
    17581 gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc
    17641 atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac
    17701 atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt
    17761 cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca
    17821 tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg
    17881 aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc
    17941 tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca
    18001 tggactcccg tcacccggat accctgctag cggcacgttc tggtagtccg ctggtgattg
    18061 gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc
    18121 gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca
    18181 tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg
    18241 acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga
    18301 acgcgataaa aaacaccctt accgggcgca tcagccacgg tcaggttgat ttaagcgagc
    18361 tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg
    18421 gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc
    18481 cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca
    18541 gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc
    18601 tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc
    18661 tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt
    18721 ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc
    18781 gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc
    18841 cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt
    18901 tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg
    18961 aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg
    19021 agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac
    19081 cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg
    19141 gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca
    19201 tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc
    19261 agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc
    19321 tggcaaaatc ggttacggtt gagtaatcac tatcatagat aaaaaagggc tgccgaccgg
//
LOCUS       DAJTSA010000079        19308 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000079 DAJTSA010000000
VERSION     DAJTSA010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19308
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.079"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..2095
                     /gene="pbpC"
                     /locus_tag="OO369_002896"
     CDS             <1..2095
                     /gene="pbpC"
                     /locus_tag="OO369_002896"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCR5983103.1"
                     /translation="ARYLEALINYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGS
                     TLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILYLNRAPFGGTLQGIGA
                     ASWAYLGKSPANLSYSEAAMLAVLPQVPSRLRPDRWPERAEAARNKVLERMAAQGVWS
                     REQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNW
                     KGRLPPRSSLAMIVVNHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVY
                     GLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPINMSEALVRSLNLPAVQVLEA
                     YGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLR
                     LQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPLAWKTGTSYGYRDAWA
                     IGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLSRSANLPEDPRPNSVT
                     RGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEGINGIRFPIWLDDKGQ
                     RVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLLLTGV
                     RDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLV
                     MDDVGQIATVKFVMQ"
     gene            2245..2676
                     /gene="ndk"
                     /locus_tag="OO369_002897"
     CDS             2245..2676
                     /gene="ndk"
                     /locus_tag="OO369_002897"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR5983104.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2826..3980
                     /gene="trmG/rlmN"
                     /locus_tag="OO369_002898"
     CDS             2826..3980
                     /gene="trmG/rlmN"
                     /locus_tag="OO369_002898"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR5983105.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4265..5278
                     /gene="rodZ"
                     /locus_tag="OO369_002899"
     CDS             4265..5278
                     /gene="rodZ"
                     /locus_tag="OO369_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR5983106.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5305..6432
                     /gene="ispG"
                     /locus_tag="OO369_002900"
                     /gene_synonym="gcpE"
     CDS             5305..6432
                     /gene="ispG"
                     /locus_tag="OO369_002900"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR5983107.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6535..7809
                     /gene="hisS"
                     /locus_tag="OO369_002901"
     CDS             6535..7809
                     /gene="hisS"
                     /locus_tag="OO369_002901"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR5983108.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7827..8447
                     /gene="yfgM"
                     /locus_tag="OO369_002902"
     CDS             7827..8447
                     /gene="yfgM"
                     /locus_tag="OO369_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR5983109.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8458..9636
                     /gene="bamB"
                     /locus_tag="OO369_002903"
     CDS             8458..9636
                     /gene="bamB"
                     /locus_tag="OO369_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR5983110.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9754..11226
                     /gene="der"
                     /locus_tag="OO369_002904"
     CDS             9754..11226
                     /gene="der"
                     /locus_tag="OO369_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR5983111.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11296..11511
                     /locus_tag="OO369_002905"
     CDS             11296..11511
                     /locus_tag="OO369_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR5983112.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="OO369_002906"
     CDS             complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="OO369_002906"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR5983113.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13040..14506
                     /gene="guaB"
                     /locus_tag="OO369_002907"
     CDS             13040..14506
                     /gene="guaB"
                     /locus_tag="OO369_002907"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR5983114.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14575..16152
                     /gene="guaA"
                     /locus_tag="OO369_002908"
     CDS             14575..16152
                     /gene="guaA"
                     /locus_tag="OO369_002908"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR5983115.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16247..16786)
                     /locus_tag="OO369_002909"
     CDS             complement(16247..16786)
                     /locus_tag="OO369_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR5983116.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16802..17320)
                     /locus_tag="OO369_002910"
     CDS             complement(16802..17320)
                     /locus_tag="OO369_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5983117.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17631..17822)
                     /locus_tag="OO369_002911"
     CDS             complement(17631..17822)
                     /locus_tag="OO369_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR5983118.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17840..17992
                     /locus_tag="OO369_002912"
     CDS             17840..17992
                     /locus_tag="OO369_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983119.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            18174..>19308
                     /gene="pdeF"
                     /locus_tag="OO369_002913"
     CDS             18174..>19308
                     /gene="pdeF"
                     /locus_tag="OO369_002913"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5983120.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT         4544 a         4701 c         5250 g         4813 t
ORIGIN      
        1 ggcacgttac cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg
       61 ggttaatcca ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat
      121 ttccggtggc agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac
      181 atttggtggc aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa
      241 gcgtgaaatt ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat
      301 cggtgcggca agttgggctt atctcggaaa atcgcctgcg aatttaagct attccgaggc
      361 ggcaatgctg gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga
      421 gcgtgccgaa gccgcgcgta ataaagtgct cgaacggatg gctgcgcaag gtgtgtggtc
      481 ccgtgagcag gtaaaagagt caagggaaga acccatctgg ctggcacccc gacaaatgcc
      541 gcaactggca ccgctgtttt cgcgcatgat gctcggtaaa agcaaaagcg acaaaatcgt
      601 tactacgctg gatgccggtc ttcaacgacg tctggaagaa ctggcgcaaa actggaaagg
      661 gcggttgcca ccgcgcagct cactggcgat gatcgtggtt aatcataccg atatgcgtgt
      721 tcgcggctgg gtgggatcgg ttgatctcaa cgatgattca cgctttggtc atgttgatat
      781 ggtcaatgcg atccgatcgc caggatcggt gctcaaaccg tttgtttatg gtctggcgct
      841 ggatgaaggc ttgatccacc cggcatcact gctgcaagac gtcccccggc gcaccggtga
      901 ttatcgacca ggtaactttg atagcggttt tcatggcccg atcaacatga gcgaggcgct
      961 ggtgcgctcg ctgaacttac ctgctgtgca ggtgctggaa gcctatggac cgaaacggtt
     1021 tgcggcaaag ttacgcaatg ttggattgcc gttatatttg cccaacggcg ctgcgccgaa
     1081 tctttcactc attctcggcg gcgctggtgc aaaactggaa gatatggcgg cagcgtatac
     1141 cgcgtttgct cgccacggca aggcaggcaa attgcgctta cagcctgacg atccgctgct
     1201 tgaacggcct ttaatgtcgt cgggggcggc gtggatcatt cgccggatta tggctgatga
     1261 agcgcaaccc ttgccggata gtgccttgcc gcgcgtcgcc ccactggcat ggaaaacggg
     1321 caccagctat ggctatcgtg acgcctgggc gattggggtt aacgctcgct atgtcattgg
     1381 gatctggact ggcagacctg acggcacgcc cgttgttggt cagtttggct ttgccagtgc
     1441 cgtaccattg ttgaatcagg tcaataatat cttactgtcg cgcagtgcga atctgccgga
     1501 agacccgcgc ccgaactcag tcactcgtgg cgttatctgc tggccgggcg gacagtcttt
     1561 gccggaaggt gacggtaact gtcgccgccg cctggcaacc tggctgctgg atggcagcca
     1621 gccgccaacg ctgttactgt cagagcagga agggattaat ggcattcgct tccccatctg
     1681 gctggatgac aaagggcagc gcgtcgccgc tgactgcccg caggcgaagc aagaaatggt
     1741 aaatgtctgg ccgctaccgc tggaaccctg gctgcccgca tcagaacgtc gcgctgtgcg
     1801 cttaccaccg gcctcgacaa tttgcccgcc atacggtcac gatgcccagc tccctctgct
     1861 gctgacaggc gttcgcgatg gcgcgattat taaacgttta ccgggcgcag cggaagcgac
     1921 tttgccgttg caatcaagtg gaggggcagg tgaacgctgg tggtttctga atggcgaacc
     1981 gttaactgaa cgcgggcgca acgtgaccct gcatttgacg gataaaggtg attatcaatt
     2041 gctggtgatg gatgatgtgg ggcaaatcgc gacagtgaaa tttgtcatgc aatagtctgt
     2101 tttgtccctg attgttgcta aaactcattt tattttaaaa aaaatgttac cttcatcaat
     2161 agtcaacggc cctgttgctc attataatcc gcgccatctc gtacgctggt acagacaaca
     2221 acagaacaat ttacagaggt aaaaatggct attgaacgta ctttttccat catcaaaccg
     2281 aacgcggtag caaaaaacgt cattggtaat atctttgcgc gctttgaagc tgcagggttc
     2341 aaaattgtcg gcaccaaaat gctgcatctg accgttgaac aggcacgtgg cttttatgct
     2401 gaacacgatg gaaaaccgtt ctttgatggt ctggttgaat tcatgacctc tggcccgatc
     2461 gtggtttccg tgctggaagg tgaaaacgcc gttcagcgtc accgcgatct gctgggcgcg
     2521 accaatccgg caaacgcact ggctggtact ctgcgcgctg attacgctga cagcctgacc
     2581 gaaaacggta cccacggttc tgattccgtc gaatctgccg ctcgcgaaat cgcttatttc
     2641 ttcggcgaag gcgaagtgtg cccgcgcacc cgttaataat ttcgtaaatg ccgcgtgcaa
     2701 acgtggcatc cgtgcgccag aatttgtaca atgcagcgcc cccggacgag cagccgctca
     2761 ccggggcgtt tcttttttca accctccagg ggccataacg tgtaataacg aggccggaat
     2821 agcatatgtc tgaacaatta gtcacacctg aaaacgtcac cacgaaagat ggaaaaatca
     2881 acctgctgga tctcaaccgt cagcagatgc gggagttttt taaagattta ggtgaaaaaa
     2941 ccttccgcgc cgatcaggtg atgaagtgga tgtatcacta ttgctgcgac aactttgatg
     3001 agatgaccga catcaacaaa gtgttgcgcg gcaaactgaa agaggtggcg gaaatccgcg
     3061 caccggaagt ggttgaagaa cagcgttcat ccgacggcac cattaaatgg gcgatcgccg
     3121 ttggcgatca gcgcgtcgaa acggtgtata tcccggaaga cgaccgtgcc acgctctgcg
     3181 tctcttcgca ggtggggtgt gcgctggagt gtaaattctg ttccaccgcc cagcagggct
     3241 ttaaccgcaa cctgcgggta tcggaaatta ttggccaggt gtggcgtgcg gcgaaaatcg
     3301 tcggcgcagc aaaagtcacc ggtcagcgcc caatcactaa cgtcgtgatg atgggcatgg
     3361 gcgagccgct gctcaacctg aacaacgtcg ttccggcgat ggaaatcatg cttgatgatt
     3421 tcggttttgg cctgtctaaa cgtcgcgtga cgctttccac ttccggtgta gttccggcgc
     3481 tggataaact gggcgatatg atcgacgttg cactggcaat ttccctgcac gcgccgaacg
     3541 acgaaattcg tgacgaaatt gtgccgatca acaaaaagta caatatcgaa acgttccttg
     3601 ctgcggtgcg ccgttatctg gagaaatcca acgccaatca gggccgagtc actattgaat
     3661 acgtgatgct tgaccacgtt aacgacggca ctgaacacgc gcaccaactg gcggaactgc
     3721 tgaaagatac gccgtgtaag atcaacctga tcccgtggaa cccgttcccg gacgcgccgt
     3781 atggacgcag ttcgaacagc cgtatcgacc gtttctcaaa agtgctgatg agctacggtt
     3841 tcaccactat tgtgcgtaaa acgcgtggtg atgatatcga tgctgcctgt ggtcagttgg
     3901 cgggcgatgt tatcgaccgt acgaaacgta ctctgcgtaa acggatgcag ggtgaagcca
     3961 tcgatattaa agcggtctga tagccattac gccacggtta ctttatgtac cgtggcgtaa
     4021 tcttttatga atgaagtatt aagttgcaat tgtctggtca atattaacgg tgcgtttttg
     4081 ctgactttaa ggcagtatgt aacgatgcaa cagtaagtta gcctcagtga atgtgggctt
     4141 tgtcacgagc acacagacgg tcttatactg tatgataacg gttaacttaa cggatgtttc
     4201 gcggtgtggg tgagcatgat ggttcaccgg catctcaatt ctcatttaaa cgtacctgca
     4261 gcgaatgaat actgaagcca cgcacgacca aaatgaagca cttactaccg gcgctcgcct
     4321 gcgtaatgct cgcgaacaac taggacttag tcagcaggcc gttgccgagc gactttgcct
     4381 gaaggtttcc acggtacgcg acattgaaga agataaggca cccgccgatc ttgcttcaac
     4441 attcctgcgc ggatatatcc gctcttatgc gcgtctggta catattccag aagaagaact
     4501 gctgccaggg ctggaaaagc aggctccact tcgggctgca aaagttgcgc caatgcagag
     4561 tttttccctc ggtaaacgcc gcaaaaaacg cgacggctgg ctaatgacct tcacctggct
     4621 ggtgttgttt gtggttatcg gcctgagcgg tgcctggtgg tggcaagacc acaaagctca
     4681 gcaggaagag atcaccacta tggccgatca atcttcggcg gaactgagca gtaatagcga
     4741 gcaggggcag agtgttccgt taaatacgtc gacaactaca gacccggcta caacgtccac
     4801 gccgccagcg tctgtggata ctaccgcaac caacacgcaa acacctgccg taactgcgcc
     4861 agcaccagct gttgatccgc aacagaatgc ggttgtttcg ccttcgcagg caaatgttga
     4921 taccgccgcg accccggtac caacggcagc aacaacgcca gatggtgctg cgccgttgcc
     4981 aaccgatcag gctggcgtga ccacgccggc ggctgatccg aatgcgctgg tgatgaactt
     5041 tactgccgat tgctggctgg aggtcactga tgctaccggt aaaaaattgt ttagcgggat
     5101 gcagcgtaaa gacggtaatt tgaatttaac cggccaggca ccgtataaac tgaaaattgg
     5161 tgcgccagcc gcagtacaga tccagtatca agggaaacct gtcgatctga gtcgttttat
     5221 cagaactaac caggttgcgc gtctgaccct caatgccgaa caatcaccgg cgcagtaaca
     5281 gacgggtaac gcgggagatt tttcatgcat aaccaggctc caattcaacg tagaaaatca
     5341 acacgtattt acgttgggaa tgtgccgatt ggcgatggtg ctcccatcgc cgtacagtcc
     5401 atgaccaata cgcgtacgac agatgtcgaa gcaacggtca atcaaatcaa ggcgctggaa
     5461 cgcgttggcg ctgatatcgt ccgtgtctcc gtaccgacga tggacgcggc agaagcgttc
     5521 aaactcatca aacagcgggt taacgtgccg ctggtggctg acatccactt cgactatcgc
     5581 attgcgctga aagtagcgga atacggcgtc gattgtctgc gtattaaccc tggtaatatc
     5641 ggtaatgaag agcgtattcg catggtggtt gactgtgcgc gcgataaaaa cattccgatc
     5701 cgtattggcg ttaacgccgg atcgctggaa aaagatctgc aagaaaagta tggcgaaccg
     5761 acgccgcagg cgttgctgga atccgccatg cgtcatgttg atcatctcga tcgcctgaac
     5821 ttcgatcagt tcaaagtcag cgtgaaagcg tctgacgtct tcctcgctgt tgagtcttat
     5881 cgtttgctgg caaaacagat cgatcagccg ctgcatctgg ggatcaccga agccggtggt
     5941 gcacgcagcg gggcagtaaa atccgccatt ggtttaggtc tgctgctgtc ggaaggcatc
     6001 ggcgacacgc tgcgtgtatc gctggcggcc gatccggtcg aagagatcaa agtcggtttc
     6061 gatattttga aatcgctgcg tatccgttcg cgagggatta acttcatcgc ctgcccgacc
     6121 tgttcgcgtc aggaatttga tgttatcggt acggttaacg cgctggagca acgcctggaa
     6181 gatatcatca ctccgatgga cgtttcgatt atcggctgcg tggtgaatgg cccaggtgag
     6241 gcgctggttt ctacactcgg cgtcaccggc ggcaacaaga aaagcggcct ctatgaagat
     6301 ggcgtgcgca aagaccgtct ggacaacaac gatatgatcg accagctgga agcacgcatt
     6361 cgtgcgaaag ccagtcagct ggacgaagcg cgtcgaattg acgttcagca ggttggaaaa
     6421 ataataacgt gatgggaagc gcctcgcttc ccgtgtatga ttgaacccgc atggctcccg
     6481 aaacattgag ggaagcgttg agggttcatt tttatattca gaaagagaat aaacgtggca
     6541 aaaaacattc aagccattcg cggcatgaac gattacctgc ctggcgaaac ggccatctgg
     6601 cagcgcattg aaggcacact gaaaaacgtg ctcggcagct acggttacag tgaaatccgc
     6661 ttgccgattg tagagcagac cccgctattc aaacgtgcga ttggtgaagt caccgacgtg
     6721 gttgaaaaag agatgtacac ctttgaggat cgcaatggcg acagcctgac tctgcgccct
     6781 gaagggacgg cgggctgtgt acgcgccggc atcgagcatg gtcttctgta caatcaggaa
     6841 cagcgtctgt ggtatatcgg gccgatgttc cgtcacgagc gtccgcagaa agggcgttat
     6901 cgtcagttcc atcagttggg ctgcgaagtt ttcggtctgc aaggtccgga tatcgacgct
     6961 gaactgatta tgctcactgc ccgctggtgg cgtgcgctgg gtatcttcga acacgtaact
     7021 cttgagctga attctatcgg ttcgctggaa gcacgcgcca attaccgcga tgcgctggtg
     7081 gcattccttg agcagcataa agaaaagctg gacgaagact gcaaacgccg catgtacact
     7141 aacccgctgc gcgtgctgga ttcaaaaaat ccggaagtgc aggcgcttct caacgacgct
     7201 ccggcattag gtgattatct ggacgaggaa tctcgtgagc attttgccgg tctgtgcaaa
     7261 ctgctggaga gcgcggggat cgcttacacc gtaaaccagc gtctggtgcg tggtctggat
     7321 tactataacc gtaccgtttt cgagtgggtg actaacagtc tcggctccca gggcaccgtg
     7381 tgtgcaggcg gtcgttatga cggtcttgtg gaacaactgg gcggtcgtgc aacaccggct
     7441 gtcggttttg cgatgggcct cgaacgtctt gtattgttag tacaggccgt taatccggaa
     7501 tttaaagccg atcctgttgt cgatatatac ctggtggctt caggtgctga tacacaatct
     7561 gcggctatgg cattagctga gcgtctgcgt gatgaattac tgggcgtgaa attgatgacc
     7621 aaccacggcg gcggcaactt taagaaacag tttgcccgtg ctgataaatg gggtgcccgc
     7681 gttgctgtgg tgctgggtga gtctgaagtg gctaacggca cagcagtagt gaaggatttg
     7741 cgctctggtg agcaaacggc agttgcgcag gatagcgtag ccgcgcattt gcgcacgtta
     7801 ctgggttaag gaaggagaag gacagcgtgg aaatttacga gaacgaaaac gaccaggtag
     7861 aagcggttaa acgctttttt gctgaaaatg gcaaagcact ggctgttggg gggattttgg
     7921 gcgttggcgc actgactggc tggcgctact ggaacagcca tcaggttgat tctgcacgct
     7981 ccgcttctct tgcctatcaa aatgcggtta ccgcagtgag cgaaggcaaa ccggatagca
     8041 tcccggcggc ggaaaaattt gctgctgaaa ataaaaatac ttatggtgcg ctggcttctt
     8101 tggaacttgc gcagcaattt gttgacaaaa atgaactaga gaaagctgcc gcccagttac
     8161 aacaggggct ggcagacacg agcgatgaaa atctcaaagc cgtgataaat ctgcgtcttg
     8221 ctcgcgttca ggtacagctc aagcaggctg atgccgcgct gaaaaccctt gataccatca
     8281 aaggtgaagg gtgggctgcc attgttgccg acttgcgtgg tgaagcattg ctgagcaaag
     8341 gtgataagca aggtgcgcgt agtgcatggg aagcaggcgt gaaaagcgat gttactccgg
     8401 cactgagcga aatgatgcag atgaaaatta ataatttgtc catctgagag ggacccgatg
     8461 caattgcgta aattactgct gccaggactg ctttccgtta cccttttaag cggctgttcg
     8521 ctgtttaaca gcgaagaaga tgtggtaaag atgtccccat tgccaaccgt tgaaaaccag
     8581 tttacgccga ccacggcgtg gagcacttcc gttggtagcg gcattggcaa cttctattcc
     8641 aatcttcatc cggcactggc ggacaacgtt gtctatgcag cggaccgcgc tggtttggta
     8701 aaagcgctga atgcggatga tggcaaagaa atctggtctg tcagcctggc cgagaaagat
     8761 ggctggttct ctaaagagcc tgcattactt tctggcggtg tgaccgtgtc tggtgggcat
     8821 gtctacattg gcagcgaaaa ggcgcaggtt tacgcgctga ataccagcga tggtactgtg
     8881 gcatggcaaa ctaaagtcgc gggtgaagca ctttcgcgcc cggtggtcag cgacggtctg
     8941 gtgttaatcc acaccagtaa cggtcagtta caagcgctga acgaagctga cggcgctgtc
     9001 aaatggacag ttaacctcga tatgccttcg ctctctttgc gtggcgagtc tgcgccggca
     9061 acggcttttg gtgcggccgt cgtgggtggc gataatggtc gcgtcagcgc agtgctgatg
     9121 gaacagggcc agatgatttg gcagcagcgt atttcccagg cgaccggttc taccgaaatt
     9181 gaccgtctga gcgatgttga cacgactccc gtcgttgtta acggcgttgt tttcgcgctg
     9241 gcctataatg gtaacctgac ggcgcttgat ctgcgcagtg gtcagattat gtggaaacgc
     9301 gaactgggtt cggtgaatga tttcatcgtc gatggcaatc gcatctatct ggtcgatcaa
     9361 aatgaccggg tgatggcgtt gaccattgat ggcggcgtta cgctgtgggc acaaagcgat
     9421 ctgctgcatc gcctgctgac ttctccggtg ctgtataatg gcaacctggt ggtcggtgac
     9481 agtgaaggtt atctgcactg gattaacgtc gaagatggtc gtttcgttgc ccagcaaaaa
     9541 gttgatagtt ccggtttcca gactgaaccg gttgccgctg acggcaaact gctgatccag
     9601 gcaaaagacg gaaccgtgta ctctattaca cgttaatcgt ctctgtcgtt cactttgaaa
     9661 acggctcctg gacaggggcc gttttcctgt ttttaacaac gacgcgaata tagcgtgcgt
     9721 tgtctgatga tttataaaaa tgaggcttta aacatggtac ctgtggtcgc gcttgtcggg
     9781 cgccctaacg taggaaaatc cacgttattt aaccgtctaa ctcgcacccg agatgcgctg
     9841 gttgcggatt tcccgggtct gactcgtgac cgtaagtacg gtcgtgcgga aattgaaggc
     9901 cgtgagttta tctgtattga taccggcggg attgatggca cagaagacgg tgtagaaacc
     9961 cgcatggcgg aacagtcgct gctggcgatt gaagaagcgg acgtcgtact gtttatggtg
    10021 gatgcgcgcg cgggcctgat gccggcagat gaagcgattg ccaaacatct gcgctcccgt
    10081 gaaaaaccga ccttcctggt ggtaaacaaa actgacggtc tggatcccga tcaggcagtg
    10141 gttgatttct actcgcttgg tttaggtgaa atctacccga tcgccgcgtc tcacggtcgt
    10201 ggcgtattaa gtctgctgga gcatgtgctg ctgccgtgga tggaagatct cgcaccgcaa
    10261 gaggaagtcg acgaagacgc tgaatactgg gcgcaatttg aagcggaaga gaacggcgaa
    10321 gaagaagagg aagacgactt cgacccgcaa agtctgccga tcaaactggc gattgtgggt
    10381 cgtccgaacg taggtaagtc tacactcact aaccgtattc ttggagaaga gcgcgttgtt
    10441 gtttacgaca tgcctggcac gacgcgtgac agcatctaca tcccaatgga acgcgatgga
    10501 cgtgagtatg tgctcattga caccgctggc gtacgtaaac gcggcaaaat caccgatgct
    10561 gtagagaaat tctccgtaat caaaacgttg caggccattg aagacgccaa cgtggtgatg
    10621 ttagtgattg atgcgcgcga aggtatttcc gatcaggatc tctcgctgct gggctttatt
    10681 ctcaatagtg ggcgctcact tgtcattgtg gtgaataagt gggatggcct gagccaggaa
    10741 gtgaaagagc aggtgaaaga aacgctggac ttccgtctgg gctttatcga ttttgctcgt
    10801 gtgcacttta tctctgcctt gcacggcagt ggtgttggta acttgtttga atcagtacgt
    10861 gaagcgtatg acagctccac ccgtcgtgtg gggacctcta tgctgacgcg catcatgacg
    10921 atggctgttg aagatcacca accgccgctg gtacgcggtc gtcgtgtgaa gctgaaatat
    10981 gcccacgctg gtggttataa cccgccgatt gtggtgattc acggtaatca ggtgaaagac
    11041 ctgcctgatt cctacaagcg ctacttgatg aactacttcc gcaaatcgct ggacgtaatg
    11101 ggatcgccga ttcgtattca gttcaaagaa ggggaaaacc cgtatgcgaa caagcgtaac
    11161 accctgacgc caacccagat gcgtaaacgt aagcgtctga tgaagcacat caagaaaaat
    11221 aaataattat ccgtaagtat tttatctgcc attccctcta tgaatgtaga gggaatggct
    11281 accggaggaa ttcatatgga actgcattgt ccaaaatgtc agcatgtgct tgatcaggat
    11341 aacggccatg cgcgttgccc atcctgcggg gaatttattg aaatgaaagc tctttgtcca
    11401 gactgccatc aaccgttgca ggtgttaaaa gcctgcggtg cagtagatta cttctgtcag
    11461 cacgggcatg gcttgatatc gaaaaaacgc gttgaattcg tgctggctta atgaaccttt
    11521 ttacgcgatt tttttaccgg ctggatgttt tttacttcac tttctatcca gccgtcttcc
    11581 agacgtgtgg ttagcatttc acccgtttta acttgcttca cttttttcag tacattgccg
    11641 tcagtagcag tagtaacgct gtatccacgc gccagcgttg acagtgggct tacggcttcg
    11701 aggtgcgtta ctgcattacc gaaacgttca cgcgtggcgc taagctgtgc gcgcaggatt
    11761 tctgctaaac gatattccag ttgctgaatg cgcgtttgcg tgcgatgaat cttcggctgt
    11821 ggattttgct gattcagccg ctgtgttaat cgctgctgct gttgcccggc acgcttaagc
    11881 tgattttcca gcgcaaagct catccgtttt tgcaggcgtt caagcatggt ttgctggcgt
    11941 gccagccgga gctgcggatg ctgctgctgc aagcgatgat gaatctgcgt aaagcgacgt
    12001 gtacggttgg cgagataata atccatcgcc atctccagcc gttgacgggc cgattgcacc
    12061 tggcgcagta actcttgctg attacggctc actacttcag cggcggcaga cggcgttggc
    12121 gcacgcagat cggcaacaaa atcggcaatg gtcacatccg tctcatgccc gacggcgctg
    12181 acgaccggaa tgcggctggc aaaaatcgcc cgcgctacgc gttcgtcgtt aaaactccat
    12241 aaatcttcca gcgaaccgcc gccgcgccca acgatcaaca cgtcgcactc attgcgctga
    12301 ttcgccagtt caatggcgcg aacaatttgc cccggcgcgt catcgccctg aacggaggtg
    12361 gggtagatga tcaccggtag agaaggatcg cgacgtttta acacatgcaa aatatcatgt
    12421 agcgcagcac cggtttttga ggtgatcaca ccaacgcaat gcgcagggga gggaagtggt
    12481 tttttgtatt gctgatcgaa caaacattca gcctgcaact tcgctttgag ctgttcgtac
    12541 ttctgttgca gcagcccttc accggccggc tgcatactct caacgattat ctggtagtcg
    12601 ccgcgcggct cgtagagcgt aatattggcg cgaactaaaa cttgttgccc atgctgcgga
    12661 cggaaggtga cccggcggtt gctgttgcgg aacatcgcgc agcgtacctg ggcggtgtcg
    12721 tctttgagtg taaagtacca gtgaccggaa gccggttgtg tgaaattaga gatttcgccg
    12781 ctgatccaaa cctgtcccat ctcatgctca agcagcagac gaaccgtttg attcaggcga
    12841 ctaacggtaa aaattgcagg ggattgagaa ggtaacatgt gagcgagatc aaattctaaa
    12901 tcagcaggtt attcagtcta tagtaacccg cccttcgggg atagcaagca ttttttgcaa
    12961 aaaggggtag atgcaatcgg ttacgctctg tataatgccg cggcaatatt tattaaccac
    13021 tctggtcgag atattgccca tgctacgtat cgctaaagaa gctctgacgt ttgacgacgt
    13081 tctcctcgtt cctgctcatt ctaccgttct gccgaatact gctgacctca gcacccagct
    13141 gacgaaaact attcgtctga atatccctat gctttccgca gcaatggata ccgtaacgga
    13201 agcgcgcctg gctattgctc tggctcagga aggcggtatc ggctttatcc acaaaaacat
    13261 gtccattgaa cgccaggcag aagaagttcg ccgtgtgaaa aaacacgaat ctggtgtggt
    13321 gactgatccg cagactgttc tgccaaccac gacgctgcgc gaagtgaaag aactgaccga
    13381 gcgtaacggt tttgcgggct atccggtcgt taccgaagaa aacgaactgg tgggtattat
    13441 caccggtcgt gacgtgcgtt ttgttaccga cctgaaccag ccggttagcg tttacatgac
    13501 gccgaaagag cgtctggtca ccgtgcgtga aggtgaagcc cgtgaagtgg tgctggcaaa
    13561 aatgcacgaa aaacgcgttg aaaaagcgct ggtggttgat gacgaattcc acctgatcgg
    13621 catgatcacc gtgaaagact tccagaaagc ggaacgtaaa ccgaacgctt gtaaagacga
    13681 gcaaggccgt ctgcgtgttg gtgctgcagt tggcgcaggt gcgggtaacg aagagcgtgt
    13741 tgacgcgctg gttgccgctg gcgttgacgt tctgctgatc gactcctccc acggtcactc
    13801 agaaggcgtt ttgcagcgta tccgtgaaac ccgtgctaaa tatccggatc tgcaaatcat
    13861 cggcggcaac gtggcaacag ctgcaggtgc acgcgctttg gcagaagctg gttgcagcgc
    13921 ggttaaagtc ggtatcggcc ctggttctat ctgtacgact cgtatcgtta ctggcgtagg
    13981 tgttccgcag atcactgccg ttgctgacgc agttgaagcc ctggaaggca ccggtattcc
    14041 ggttatcgca gatggtggta ttcgcttctc cggcgacatc gccaaagcta tcgccgctgg
    14101 cgcaagcgcg gtgatggtag gttccatgct ggcgggtact gaagaatctc cgggtgaaat
    14161 cgaactctac cagggccgtt cttataaatc ttaccgtggt atgggttccc tgggcgcgat
    14221 gtccaaaggt tcctctgacc gttatttcca gagcgataac gctgccgaca aactggtgcc
    14281 ggaaggtatc gaaggtcgcg tagcttataa aggtcgcctg aaagagatca ttcaccagca
    14341 gatgggcggc ctgcgctcct gtatgggtct gaccggctgt ggtactatcg acgaactgcg
    14401 tactaaagcg gagtttgtac gtatcagcgg tgcgggcatt caggaaagcc acgttcacga
    14461 cgtgaccatt actaaagagt ccccgaacta ccgtctgggc tcctgattct cttcgcccga
    14521 tttcatgtcg ggcgatttat attatctgtt tcacttgcct cggaataagc gtcaatgacg
    14581 gaaaacattc ataagcatcg catcctcatt ctggacttcg gttctcagta cactcaactg
    14641 gttgcgcgcc gcgtgcgtga gctgggtgtt tactgcgaac tgtgggcgtg ggatgtgaca
    14701 gaagcacaaa ttcgtgactt caatccaagc ggaattattc tttccggcgg cccggaaagt
    14761 actactgaag aaaacagtcc gcgtgcgccg cagtatgtct ttgaagcagg cgtaccgata
    14821 ttcggcgttt gctatggcat gcagaccatg gcaatgcagt tgggcggtca cgttgaagcc
    14881 tctaacgaac gtgaatttgg ctacgcgcag gtagaagtcg taaacgacag cgcactggtt
    14941 cgcggtatcg aagatgcgct gaccgcagac ggtaaaccgc tgctcgatgt ctggatgagc
    15001 cacggcgata aagttaccgc tatcccgtcc gacttcgtca ccgtagccag caccgaaagc
    15061 tgcccgtttg ccattatggc taacgaagaa aaacgcttct atggcgtaca gttccacccg
    15121 gaagtgactc acacccgcca gggtatgcgc atgctggagc gttttgtgcg tgatatctgc
    15181 cagtgtgaag ccctgtggac gccagcgaaa attatcgacg atgctgtagc tcgcatccgc
    15241 gagcaggtag gcgacgataa agtcatcctc ggcctctctg gtggtgtgga ttcctccgta
    15301 accgcaatgc tgctgcaccg tgctatcggt aaaaacctga cttgcgtatt cgtcgacaac
    15361 ggcctgctgc gtctcaacga agcagagcag gttctggata tgtttggcga tcactttggt
    15421 cttaacattg ttcacgtacc ggcagaagat cgcttcctgt cagcgctggc tggcgaaaac
    15481 gatccggaag caaaacgtaa aatcatcggg cgcgttttcg ttgaagtatt cgatgaagaa
    15541 gcgctgaaac tggaagacgt gaagtggctg gcgcagggca ccatctaccc tgacgttatc
    15601 gaatctgcgg cttctgcaac cggtaaagca cacgtcatca aatctcacca caacgtgggc
    15661 ggcctgccga aagagatgaa gatgggcctg gttgaaccgc tgaaagagct gttcaaagac
    15721 gaagtgcgta agattggtct ggagctgggc ctgccgtacg acatgctgta ccgtcatccg
    15781 ttcccgggac caggccttgg cgttcgtgtt ctgggtgaag tgaagaaaga gtactgtgac
    15841 ctgctgcgcc gtgctgacgc catcttcatt gaagaactgc gtaaagcgga cctgtacgac
    15901 aaagtcagcc aggcgttcac cgtcttcctg ccagtacgtt ccgttggcgt aatgggcgat
    15961 ggtcgtaagt atgactgggt tgtctctctg cgtgctgtcg aaaccatcga ctttatgacc
    16021 gcacactggg cgcatctgcc gtacgatttc ctcggtcgcg tttccaaccg cattatcaat
    16081 gaagtgaacg gtatttcccg cgtggtgtat gacatcagcg gcaagccgcc agctaccatt
    16141 gagtgggaat gattttgatc cctgcactat gaatgaacaa aaccctccgt tactacagag
    16201 ggttttttat cttcaagaat cataggcttg aagttactaa catcgattaa ttaaaccagc
    16261 tgtccgattt gttctcttct gctttgccca cgcttttcat cagatcgcga ccgccttcag
    16321 tcatatttct gtttgcgtca gcttcagatt gcaccacatc ggtttgcgca gctttgtgct
    16381 tcagttcctg atcgataaat tcgttttcgc gcttaacgcg ggcttcttct ttcgccagcg
    16441 ccagtttttg tttctgaatc tctaagctgc gtagctcatc ttcataactt tgatcgcgtt
    16501 ttttgtccgc agtggcttcg gcgtccagtt tatcctgacg agctttctta tttgccgctg
    16561 ccgttgccgc tcttttgttc gcggcggcct gggcatttgc gcgacgttgc ttctcttgct
    16621 ggatttccct gttgcgctcc gcgacccatt cgtcatgctg cctttgctct tcatttttac
    16681 cttgctgttc cgcttctgcg acagcagaga gttgatcctg caatgatgag gctatagccg
    16741 gatagcttaa ggaggctaag atggcgcaaa gaaaaacttt cttcatgact cctcctgatt
    16801 attagctctt ttcaggacat ttggtatttg gctgaatacg cgtttcgtta tacgttgtgg
    16861 taataacaac ggctaaacct gtcgtaaact ggcactcttt accctcctgg gtggaggtat
    16921 acactttggt gccttcctta tatgttaaag aaacaccttc cactaaggtt ttatcattca
    16981 ccatagaacc cgctgccgcg cctacagctc cgccgccaac tgcccctgcc gtcgttccgg
    17041 aattgctgcc agaaccgacg ttgtggccga taacaccgcc agcgactgcg ccaataagcg
    17101 cgccgaaggc ttgtgcgttc cgtttatttt gggagttgtc tacggcaact tttgcgggaa
    17161 gaatggaaat aatattaacg gttttagttt cttgtttggt attcagttga tcggtttgat
    17221 aaacatcggc ggcatgatcg tcagcatttg actggcatcc tgccagagtg aatgacgcta
    17281 acattgccac aggcagaaga cattttttaa atttcatcac tattccttgt tatacacatt
    17341 acgaaaaata agacatttaa gatcatcaaa agaggaaagt aaatgtattg cgtataaagg
    17401 gatagattaa atttattaaa attcacgcag ggaataattt ataaaaatat atcattacaa
    17461 catatatatt aattttatat ttatttcgca gccaaacagg ataaacatca gtcaggacaa
    17521 agacgtaaaa tttaagatga aagaatatct gccgctttga taatcccaaa gagcaaaagc
    17581 atccgacagc gaagtgccgt tatgttctgt gaaagtggag aaggtaaccg ctaacgttgt
    17641 accggtgatt cgacggagag tgtggattgc tgagcttctt ttttgctctg atggtgctgc
    17701 caggcaccga cggacgagta gataaaacgg ccaaagaaga agataaagct gatgagcagt
    17761 acgatacggg tcatgcgact gttaaatcgg tgtcgttttc gcatactagt tgcctgactc
    17821 acaaaaggtt ccttgaagta tgtcccacgc tgtggacggt acttacatta aggcacaaca
    17881 ggacaaaatg gtcaattctt cgttatgtaa aaaagcgtca gtggatacat attttaatgt
    17941 tatggaagtt aatttaatta tttacaatga tgatgtaata atgatgaatt aacatgataa
    18001 acgtaataat tcattaatca gggttatttt gtttgatata tatcaattgg aactttcata
    18061 tgacacttag aatcaatgct ctctatagtg atgaataatc atcattcgaa gtcaggtggg
    18121 atgcctgtct gaatacacct ccttcaggat gtgggggatt ctgctgagca tctatgaaac
    18181 tgaatgcaac ttatataaaa atacgtgata aatggtgggg gcttccgctg ttcctgcctt
    18241 ctttaatctt gcccattttc gcccacatta atactttcgc gcatatttct tccggtgagg
    18301 tttttctctt ttatctgcct ttggcactga tgatcagcat gatgatgttt ttcagctggg
    18361 cggcattgcc agggatcacc ttagggattt ttgtccgcaa atatgcagag ctgggttttt
    18421 acgaaacgct ctcattaacg gctaatttta ttatcattat cattctctgt tggggcggtt
    18481 acagggtctt tactccccgg cgtaacaacg tttcacatgg tgatacccgt ttaatttccc
    18541 agcgtatatt ctggcagata gtgtttcctg caacgctgtt tctgatactt ttccagtttg
    18601 ctgcatttgt aggattactg gcgagcagag aaaatctggt cggtgtcatg ccttttaacc
    18661 tcgggacttt aatcaattat caggccttac tggtgggtaa tctgatcggt gtcccgctgt
    18721 gctacttcat cattcgggta gtgcgaaatc cattttattt acgtagctat tattcgcaat
    18781 taaaacagca ggttgatgcc aaagtcacca aaaaagagtt cgcgctctgg ctactggcat
    18841 taggtgcttt attgttgctg ttatgcatgc cgttaaatga aaaaagcaca atttttagca
    18901 ccaattatac cttgtcatta ttgctgcccc tgatgatgtg gggagcaatg cgctatggtt
    18961 ataagctgat ttcgctgctc tgggcggtcg tgttgatgat cagcatccac agctatcaaa
    19021 attacattcc catttatcct ggctatacca cgcagctgac cataacctcc tccagttatc
    19081 tggtattctc ttttattgtc aattatatgg ctgtactggc aactcgtcag cgagcggtag
    19141 tcagacgcat tcagcggctt gcgtatgtgg acccggtggt tcatctgcca aatgttcgcg
    19201 ccctgaatcg cgcgttgcgt gatgccccct ggtctgcgct ttgttattta cgcatccctg
    19261 gcatggaaat gctggttaag aactatggca tcatgctgcg gattcaat
//
LOCUS       DAJTSA010000080        18803 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000080 DAJTSA010000000
VERSION     DAJTSA010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.080"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(139..3654)
                     /locus_tag="OO369_002914"
     CDS             complement(139..3654)
                     /locus_tag="OO369_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5983121.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4107..5369)
                     /locus_tag="OO369_002915"
     CDS             complement(4107..5369)
                     /locus_tag="OO369_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5983122.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(5567..5642)
                     /locus_tag="OO369_002916"
     tRNA            complement(5567..5642)
                     /locus_tag="OO369_002916"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5607..5609),aa:Phe,seq:gaa)
     gene            complement(5748..6455)
                     /locus_tag="OO369_002917"
     CDS             complement(5748..6455)
                     /locus_tag="OO369_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR5983123.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMKSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            6853..8988
                     /gene="speC"
                     /locus_tag="OO369_002918"
     CDS             6853..8988
                     /gene="speC"
                     /locus_tag="OO369_002918"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR5983124.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OO369_002919"
     CDS             complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OO369_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR5983125.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OO369_002920"
     CDS             complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OO369_002920"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR5983126.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(11639..11914)
                     /locus_tag="OO369_002921"
     CDS             complement(11639..11914)
                     /locus_tag="OO369_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR5983127.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OO369_002922"
     CDS             complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OO369_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR5983128.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            13155..13874
                     /gene="trmB"
                     /locus_tag="OO369_002923"
     CDS             13155..13874
                     /gene="trmB"
                     /locus_tag="OO369_002923"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR5983129.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            13874..14200
                     /locus_tag="OO369_002924"
     CDS             13874..14200
                     /locus_tag="OO369_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR5983130.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            14384..15103
                     /locus_tag="OO369_002925"
     CDS             14384..15103
                     /locus_tag="OO369_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR5983131.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            15279..16325
                     /gene="ansB"
                     /locus_tag="OO369_002926"
     CDS             15279..16325
                     /gene="ansB"
                     /locus_tag="OO369_002926"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR5983132.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASVNRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            16442..17404
                     /locus_tag="OO369_002927"
     CDS             16442..17404
                     /locus_tag="OO369_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR5983133.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(17505..>18803)
                     /locus_tag="OO369_002928"
     CDS             complement(17505..>18803)
                     /locus_tag="OO369_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR5983134.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILES
                     VVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIF
                     MDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGV
                     KIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         4899 a         4583 c         4643 g         4678 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 acttgtcaga aacatcaatc ttcgtcatca tttacaaaga aaaataatta gttaaagcag
     4081 tccgggctga ttgcccggac tgcttactat tttaatggat tgttaatctt tgcatattca
     4141 aacgggctga tacccttctc ccggttcgca tctagaaaat cggcccacca ttgcaacatc
     4201 agtcgccgtt cttccagatg ttctgcttta tggatatacg cagcacgtac accattacgc
     4261 tcctgatggc tcatctgacg ttccacagca tcgcgggacc acaaacctga ttcaatcaat
     4321 gcactgcacg ccatcgcccg gaatccatgg ccacagactt cctgggttgt gtcatacccc
     4381 atcaccctca gggctttatt tacagtattt tcactgattg gtttacgcgg gtcatgtgca
     4441 cccgtaaaga tcagaccatt ttcaccagcc catgtctgaa gttctgccag tatggctaca
     4501 acctgtttgc tgagtggcac aagatgtggt gttcgcatct tggatccccg gtgtgagaat
     4561 tttacgcctg gcagaggttc gcgttcggct ggaatagtcc acatggaatt gtcaatatca
     4621 atttccgacc agcgggcata tctgagttca ctggaacgaa taaaaaccag taaattcagc
     4681 ataactgcca gtcgggtgac aggctggcct ttataaccgt caatacgcga cagaagttca
     4741 ggcaggcgtg aaagctccag agcagggcga tgctggcgtt ttaccgtggt caatgcacca
     4801 gccatatcca gagccgggtt atatctgatg atgccagact gtacggcgta acgcattaca
     4861 gcagatattc tttgctgcag acgactggcg atttcataaa tttgttttgc ttctgcagca
     4921 cgaacaggga taagcaggtc cggggtattg agtgttgtga tatcccggtt gccgataaag
     4981 gggaaaacgt gtgtctcgag gcttttgagt actttatcgc tgtgtgattg tgcccattgt
     5041 ttattactgg catgccagcg tcgtgcaata tgttcaaaag agagctgtaa atcagggaca
     5101 acttttttaa ctcttttctg ttcgcaaggg tcaatcccct ctgaaatcag ttttcgggcc
     5161 tcatcccgtt ttgtacgagc ttcggagaga gaaacttctg gatacacacc aagtgccaga
     5221 gtcttctcct tacccagaat acgataacgg agacgccagt aacgggaacc attaggatga
     5281 accagcagga acatgccggc cccgtcagtg agtttataag ccttgtcagt gggctttgca
     5341 gcccggattt ttgcgtcagt cagtgccata gttcagcctt ctgaatgcgt taaagagggt
     5401 acaaattctg tcgaactggg gtatacccgc aattataccc acacaatggg gttgatgtgg
     5461 ggggaatctg gttgagttcg gtagaattga tttggaggaa gaacacttaa atcatggtgt
     5521 tctgaaacga aaaacggacc tccgtggagg tccgttgata tgaatttggt gcccggactc
     5581 ggaatcgaac caaggacacg gggattttca atcccctgct ctaccgactg agctatccgg
     5641 gcaacggggc gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac
     5701 tgcagactgc acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag
     5761 gcggtccagg cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc
     5821 gggaacattt taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg
     5881 gcgctgaagt ctgccatcat cgacggtgtg atcagcggta atatcagcgc ggcagcccac
     5941 gccagcgtta actggatgat cagtaatggg atactaatta ccgacaccgc aatacccagc
     6001 gagcaggcga agatcatcgc cgtaaagaaa tcaagaaatg acttggcgat taaaatactc
     6061 ggatcgccgg tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac
     6121 aggacaataa tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag
     6181 tgacgaaaca gattttgtgc tttggcgact gctgtattga cacctttctc cagcaaacaa
     6241 atttcgccaa ttagagcgcc aagtagcgta gctaaaacca tcgctggaag gttggcgcat
     6301 ttcacaacca gtaaaatacc aatccccagc gatgccagac caaaaataga tttcatggag
     6361 acgcggatac gttccggtaa gcgttggctg agcagtgcgc cgagaacgcc acccagtaag
     6421 actgcactag cgttgataaa agggccgatg accacaagag ttcctgttag ctgtattgtt
     6481 tgattttctt attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg
     6541 cttgcacgcc ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt
     6601 ttgcccgatg cacgccatct ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt
     6661 ttccctttct atgttactgc tcatgcggtg aagttaacgc acgctcactg caggacaaca
     6721 gtaaaatcag agcgtttctg cttttactga tgtctggcgg tcggagctgg tgaccagttt
     6781 gacccatatc tcatggggca gggttttcca ccttgtccgg tattcttact tccccgaaac
     6841 gggtttgcgc ttatgaaatc aatgaatatt gccgccagta gtgaactggt atcccgactt
     6901 tcttctcatc gccgcgtggt ggcgttggga gatactgatt ttacggacgt cgcggcagtc
     6961 gtcattaccg ctgcggatag tcgcagtggc attcttgcgt tgcttaagcg caccggtttt
     7021 catctaccgg tgtttttgta ttccgaacat gctgttgaat tacctgcggg cgttacggcg
     7081 gtaatcaacg gcaacgagca gcagtggctg gagctggaat ccgcagcctg tcagtatgaa
     7141 gagaatttgc tgccaccgtt ttatgacacg ctgacgcagt acgttgagat gggcaacagc
     7201 acctttgctt gccctggaca tcaacatggt gcgttcttta aaaagcatcc tgccggacgc
     7261 catttttacg atttctttgg tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta
     7321 aaattgggcg atctgcttat tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc
     7381 aaagtctttc atgccgataa aacctatttt gtgctgaacg gcacatcggc agcgaataaa
     7441 gtggtgacga atgcgctgtt aacgcgtggc gatctggtgc tcttcgaccg taacaaccat
     7501 aagtcgaatc atcacggcgc gctgattcag gcggggacga cgccggtcta tctggaagct
     7561 tcacgtaacc cgtttggttt cattggcggt attgatgcgc actgttttaa tgaagagtat
     7621 ctgcgccagc aaattcgcga cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc
     7681 ctgacgatta ttcagctggg aacctatgac ggcactgtct ataacgcccg tcaggtgatc
     7741 gataccattg ggcatctgtg tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa
     7801 cagtttatct cgatgatggc ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat
     7861 ccggggattt ttgtgaccca gttggtgcac aaacagcagg ctggattctc acagacgtcg
     7921 cagatccata aaaaagataa ccatatccgc ggacaggcgc gtttttgccc gcataagcgg
     7981 ttgaataacg cctttatgct ccatgcttct accagccctt tctatccgct gtttgctgca
     8041 ctggatgtta acgccaaaat tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc
     8101 gttgagttgg gtattgagtc gcgcaaggct attcttgcgc gctgtaagct gttccgcccg
     8161 tttatcccgc ccgttgttga tggcaaattg tggcaggatt atccgacatc agtgttagcc
     8221 agcgaccgcc gttttttcag ttttgagccg ggggcgaagt ggcacggctt tgaaggatat
     8281 gccgcggatc agtattttgt tgatccgtgc aagctgttac tcactacacc aggtatcaat
     8341 gccgaaaccg gcgaatatag cgactttggc gttccggcga cgattctggc gcactatctg
     8401 cgtgagaacg gcattgtgcc ggagaagtgc gatctcaact ccattctgtt cttattaact
     8461 ccggcggaaa gccacgagaa gctggcacaa ctggtggcga tgctggcgca atttgaacag
     8521 catattgagg atgactcgtc gctggctgag gtgttgccga gcgtttataa caaatatccg
     8581 gtgcgctatc gcgactacac cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc
     8641 agtttcgacg tcaaagacct acaaaaagcg atgttccgcc agcagagttt cccgtcagtg
     8701 gtgatgaacc cccaggatgc gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg
     8761 attcgtgatg ccgaagggcg aattgcggca gaaggggcgt tgccttatcc acctggcgtg
     8821 ctttgcgtgg tacccgggga agtctggggt ggggcggttc aacgttattt ccttgcgctg
     8881 gaagaaggcg tgaatttgtt gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa
     8941 accgatgcgg atggcatgaa acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac
     9001 gggtcacctt ctggcgaccc gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc
     9061 tgtcgggaca cgaacgtgtt tatatttgaa catcgccatg aacgcgaagg ccagaaccac
     9121 ggaataacca gcgaaaatca accatacggt ctgccagtcg gtaatgccgt tttgggtgta
     9181 catctcaaca actttaccgc tcacgatgcc gccgaggata cagccgaagc cgttagtcat
     9241 catcaggaac atcccttgtg cactggcgcg aattgccggg ctaacttctt tttcgacaaa
     9301 caccgaacca gagatgttga agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac
     9361 cagcagtaca gtaccgaacg gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat
     9421 ccacgccaca atactgatca tcattacgtt cttaataccg tagcggctta agaagaacgg
     9481 gatggtcaga atgaacaggg tttcagagat ctgcgaaatc gacatgatga ttgacgcatg
     9541 ctgcacgatg aagctgctgg caaacatcgg atctttgtcg aagctgtgca ggaaggtatt
     9601 accgaacatg ttggtaatct gcagttccgc gcccagcagc attgagaaga tgaagaagat
     9661 tgccatacgc ttgtttttaa acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct
     9721 ctgattcgct tgctgtttag caaccggaat atgcggcaga gtcagggtaa acagaaccag
     9781 aatggcggaa agtgctgcgc caatatacag ctgcatgtgg cttaattcga agccagacag
     9841 gctcaccacc cacattgcca tgataaagcc gatggtgccc cagatacgga ttggcgggaa
     9901 gtcagtaacg atatccatcc cggcattttg caggcgatag taagagatgg tgttgattaa
     9961 cccaagcgtt ggcatataag caaacgagtt aatcaatatc acaaggaaca tcgcttccgg
    10021 cgtcgtgacc tgtgccgcca tgaacagcgt gatagcgcca atggtgtggc aaatggcata
    10081 tacccatttc gcacttaacc atttgtcggc cacaatcccc agcagcgcag gcataaatac
    10141 cgcggcgata cccagtgagc tataaactgc gccaatagaa gcaccgtcaa acttcagggt
    10201 aacaaacata taggagccga gggtcgtcag ccaacttccc cacagacaga actgcagaaa
    10261 agagaggatt ttcagctgca gcttaagatt catgttaatt tcctcacatc gtgatgcgga
    10321 tgagtgtttg aaaaagcaca tttgaccgtg cgagatgatg ggattctatc aacttcgcgg
    10381 actggttttt tgttacctgt ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat
    10441 tgtgatgtgg ataacatttt tgcccctgag catcgtcagg ggcggttaat ggaattatcg
    10501 gcggcggtag gatttttgcg cggtattcac tttataaaga taacggcgag attccgcaga
    10561 gggatggcgg gtcgtcagcg tctgataaac atcgcccggc gtcatggtgt taataatatt
    10621 ggcagcctga atcttatcat tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata
    10681 ggcggtgatg acggcataac gccgcgatgt tgggttatca attccgccga gataaacatt
    10741 gttcagcatc gccagatacg cggtgccggt atcaatattg ctggcaggat caaacaagaa
    10801 actgcggctc ggcgtgccgg atttcccctg cgagcggaac acatctttcc cggcagtatt
    10861 ttgtaccacc tgcattaatc ccagcgcatc ggaacggctg accgcatacg ggttaaagga
    10921 agattcagtc tgcataattg ccagaatcag cgactcatca acgccatatt tccgtgacgc
    10981 ctggcggacc atgccgagat atttgtgcgc acgtttatca aggtggttcg gcaccatgtt
    11041 aatggtgacg ctgtagatga tacgtagccc gttgctgcgg cttttcagac ggtttttcag
    11101 cagataatcc gcgaagttgc ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc
    11161 caccacctga ccgtaaagga aaggttcttt cgaaattgta atatcatcaa cgtcggaata
    11221 gagatcgacc gaactcggat cgtcacccat cagcaacgtt ttgataattg cccggcgcaa
    11281 atgcgcggca ggttctgtcc cggcgatggt ttcgatagta atcgtaccgt catcgaagtt
    11341 gatgtggctg cgggtctgat attgatcggt gtatttcacg tagtccttag gaccggcgat
    11401 caccacctct ttgaagcccc agatgttctc aatattgtgg gcaaattgcc ccatcagaat
    11461 atcaaaaccg ttggtatctt tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt
    11521 cgaacaggag atgagcaacg gcgcaatcaa agccagcgcg agatattttt tcatcattcc
    11581 gggtgcgtgt tgtgtttgtt tttatgcaag ctgccggagg cgtgctccgg cactgttttt
    11641 attttttatc ttccggcgta tagccctcga tatgcacctc tttaccctcg aacaggaagt
    11701 tgaccatctc ctgctccagc agcttgcggt gctcggcatt catcatgttg agtttctttt
    11761 cattaatcag catggtttgt ttgtgctgcc actgcgccca ggcttctttg gagatctcgt
    11821 tatagatgcg ttttcccagc tcgccggggt acagctgaaa atcctgacct tctgcttcac
    11881 gttgcaggaa agtacaaaaa atcgttctgc tcataaatca tcctctttat cgactcacgc
    11941 gctaaaccgg cgcgccagtg cgtaactgct gtaacaaacg ctccacggga gccgccaggc
    12001 caactgacgg cggttgcgct aagttatacc agagcgcatt gccttcatcc atgcagccgg
    12061 tgaatgacga cacgggaagc cacataggca caatatctaa gtggaaatgg ctgaaggtat
    12121 ggcgaaacgc ggtcagttgc gtcaggttat cggcagcaat ctgccgttgt gccagccaat
    12181 gccgcaaact ttcttcgtcg gcaaactgcg ggaaacagta taaaccgccc cacaatccgc
    12241 tcggcggacg ctgcgccagc aatacttcat cttcgtgctg taacaggaga aagtagccgg
    12301 tgcgctccgg cagcgtctgt ttcggttttt tgcccggata aagcgaccag ctattgttgg
    12361 tggcggcaat acatccgttt tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc
    12421 aaatcatcgc gcccaaatcc atcatcgcct gattaaaccg ttccacgccg accgcgggcg
    12481 tcacctgctc gctcaaactc catagtttat tctcgacctc ttttttccca ggccagccgc
    12541 ttacagcata gcagcgcgcc agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct
    12601 tacccagaga aagcgagaga atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg
    12661 cgacttcttc aaaggtttcc gggaatttac caccgtgtaa ggtcgccact tgttgtgccg
    12721 ctttatgcag gttgcgcgcg cgggcgtaat agccaagccc ggtccacaag tggagaactt
    12781 cgtcgagcgg cgcattggcg agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa
    12841 aataggggat aacggtcgca acctgagttt gttgcaacat cacttctgag agccatactt
    12901 tgtagggcgt cttgtcaatt tgccagggca gcgttttccg cccgtattta tcgtaccagt
    12961 ccagaacctg ggctgaaaat tgcgacgctt gcatggtcac cgacttcact gttgttgggg
    13021 gcaagattgc agcacagcga cggcagggtg taaaccggaa ctttccgcag ccacggcctt
    13081 atcatgcttg catccggcaa ctaactttgg ataatgcccg ttttcagaac actttcacaa
    13141 gcgactaaat ctttatgaaa aacgacgtca tttcaccgga atttgatgaa aacggccgcc
    13201 cactgcgccg tatccgtagt tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac
    13261 atgcgctgga aaactactgg ccggtgatgg gcgttgagtt cagtgaagat atgctggatt
    13321 tccccgcgct ttttggccgt gaagcgccgg tgacgcttga gattggtttt ggcatggggg
    13381 cgtcgctggt ggcaatggct aaagatcgcc ccgagcagga cttcctcggc attgaagtgc
    13441 attcaccggg cgttggtgcg tgcctggctt ctgcgcatga agaaggttta agcaacctgc
    13501 gcgtgatgtg tcacgatgcg gttgaagtgc tgcataaaat gattcctgac aattcattgc
    13561 gcatggtgca gctctttttc cctgacccgt ggcacaaagc gcgccataat aaacgccgta
    13621 tcgttcaggt gccgtttgcc gaactggtaa aaagcaaact gcagctgggg ggcgtattcc
    13681 atatggcgac cgactgggaa ccttatgcgg aacatatgct tgaagtgatg tcttctattg
    13741 acggttataa aaacctgtca gagagcaatg attacgtacc gcgtccggca tcacgtccgg
    13801 tgacgaaatt tgaacaacgt ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg
    13861 agagggtgaa ataatggcaa agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga
    13921 cgaattccag gaattaggat tttcggtggc atggcgattc ccggaaggta catcggaaga
    13981 acagattgat aaaatcgttg atgactttat taacgaggtt atcgaaccga acaaactggc
    14041 ctttgacggc agcggttatc tggcctggga aggtctgatc tgcatgcagg aaatcggcaa
    14101 atgcaccgaa gaacatcagg cgattgtgcg taagtggctg gaagagtgca aactggatga
    14161 ggtacgcacc agcgaacttt tcgacgtttg gtgggactaa gaaagcatac gggcgatgac
    14221 aaatgcaaaa ctgcctgatg cgctacgctt atcaggccta cagagatgca cgatcgagta
    14281 ggcgggataa ggcgtttacg ccgcatccgg catggaaaac gcgtactttg ttatcaatct
    14341 ggggccagca tatgctggcc tgatttgttc ttgagggaag actatgatgc gcaaaatgct
    14401 gctggcggca gcactttcag tgacggcaat gaccgctcac gccgactacc agtgcagcgt
    14461 cacgccgcgt gacgatgtga ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg
    14521 caatctggtg atcacgccag acggcaacgt gatgtataac ggtaagcaat attccctgaa
    14581 tgccgcccag cgcgagcagg cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg
    14641 gattgatgaa ggcgcgaaaa gccgcgtcga gaaagcccgt attgcgctgg ataaaattat
    14701 cgttcaggag atgggcgaca gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca
    14761 gcttaaagag cagatgaatc gcattatcga aacgcgcagc gatggcctga cgttccacta
    14821 taaagccatt gatcaggttc gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg
    14881 aattttacag gacagcatta atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa
    14941 cccattacag aatgtgctgg gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg
    15001 gaaaaagcag gaaaaagatt tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac
    15061 tctggaagat agccgcaaag ccctggtcgg gaatttaaaa taatcctcta ttttaagacg
    15121 gcataatact tttttatgcc gtttaattct tcgttttgtt acctgcctct aactttgtag
    15181 atctccaaaa tatattcacg ttgtaaattg tttaacgtca aatttcccat gcaaagctaa
    15241 gggataatgc gtagcgtcca cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg
    15301 cacttgccgc actggttatg ggttttagtg gtgcagcatt ggcattaccc aatatcacca
    15361 ttttagcaac cggcgggacc attgccggtg gtggtgactc cgcaaccaaa tctaactaca
    15421 cagcgggtaa agttggcgta gaaaatctgg ttaatgcggt gccgcaacta aaagacattg
    15481 cgaacgttaa aggcgagcag gtagtgaata tcggctccca ggacatgaac gataatgtct
    15541 ggctgacact ggcgaaaaaa attaacgccg actgcgataa aaccgacggc ttcgtcatta
    15601 cccacggtac cgacacgatg gaagaaactg cttacttcct cgacctgacg gtgaaatgcg
    15661 acaaaccggt ggtgatggtc ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc
    15721 cattcaacct gtataacgcg gtagtgaccg cagctgataa agcctccgtt aatcgtggcg
    15781 tgctggtggt gatgaacgac accgtactgg acggtcgcga tgtcaccaaa accaacacca
    15841 ccgacgtagc gaccttcaag tctgttaact acggtcctct gggatacatt cacaacggta
    15901 agattgatta ccaacgtacc ccggcacgta agcacaccag cgatacgcca ttcgatgtct
    15961 ctaagctgaa tgagctgccg aaagtcggca tcgtttataa ctacgctaac gcatccgatc
    16021 ttccggctaa agcactggta gatgcgggct atgatggcat cgttagcgct ggtgtgggta
    16081 atggtaacct gtataaatcc gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg
    16141 cagtagtgcg ttcttcccgc gtaccgacgg gtgctaccac tcaggatgct gaagtggatg
    16201 atgcgaaata cggcttcgtc gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc
    16261 tgcagctggc tctgacgcaa accaaagatc cgcagcagat ccagcagatt ttcaatcagt
    16321 actaatcacc tcgtcccggc atcgtgccgg ggctttttca cttcagactt acgtccattg
    16381 ccaattttca ttaccctaat gataatcacc ggaataaatt attccgcgcg agggttttcg
    16441 ggtgaaaaag caatggattg ttggcacggc gctgcttatg ttgatgactg gtaatgcctg
    16501 ggcagatggc gaaccgccaa ctgaaaatat cttaaaagat caattcaaaa agcagtatca
    16561 cggcattctc aagcttgatg ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc
    16621 cacctggtca gcggaaggcg atgtctcttc cagtgacgat ctctatacct gggtcggtca
    16681 gttggcagat tacgaactgc tcgaacagac ctggacgaaa gataaaccgg taaaattctc
    16741 ggcgatgtta accagtaaag gaacgcccgc gtctggctgg tcggtgaact tttactcttt
    16801 tcaggcggca gccagcgatc gtgggcgggt ggtcgacgat atcaaaacga ataataaata
    16861 tctgatcgtg aatagcgaag atttcaatta tcgctttagt cagcttgaat ctgcgttgaa
    16921 tacccagaag aactcgattc ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat
    16981 ggtggcggcg cagaaagcag cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat
    17041 gacccgcgaa gaagccttta aaaaaattca ccaacagcgt gatgagttta ataaacagaa
    17101 cgatagcgag gcgttcgcgg ttaaatatga caaagaggtt tatcaaccgg cgattgcggc
    17161 atgtcataaa cagagtgaag agtgttatga agtgccgatt cagcagaaac gagatttcga
    17221 tatcaacgaa caacggcgac agacttttct gcaatcacaa aaactcagcc gtaaattgca
    17281 ggatgactgg gtaacgctcg aaaaagggca atatccgtta acgatgaaag tttcagaaat
    17341 aaacagtaaa aaagtcgcga ttttgatgaa aattgacgat atcaaccaag ctaatgaacg
    17401 ctagaaaaaa gataccgaac aactgcggcg caatggggtg attaagtaac tcatgttaag
    17461 tgcctttgcc ggataatata aaaagttatc cggcaaatta ttttttacat tagccctagt
    17521 agctgtggaa tgaacaagga tatttgcgga atgtaagtga tcatcatcaa cgctactagc
    17581 aatgcagcat aaaatagcag caatggtttg attaaatgtt gaatcttcac acccgagata
    17641 gaacaaccaa caaatagcgc actacccacc ggcggtgtta acagcccaat acaaaggttg
    17701 gccaccatca taataccgaa atgtactggg tccattccca gctcttgtgc aatcggcaag
    17761 aagatcggtg taaagattag tacagccggt gtcatgtcca taaagatgcc gacaattaat
    17821 aagacgatat taatcagcag caatatgatc agtgggttat cagaaatccc catcaatgcg
    17881 tcgctgatca tataaggaat atcagcatta gtcattgccc aggacatgcc cacggaaaaa
    17941 ccgatcaaca gtaatacaat cgaagtggta acaaccgact ccagaatcag tttgggtaaa
    18001 tggcgccatt ttacttcccg gtaaatcagc accgagagaa taaatgtgta gacaacggca
    18061 attgctgacg cttcagttgc ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac
    18121 accagaagca aacttggtaa ggcatccaga aacgctttac aggcctgagc aaaggttgct
    18181 ctttctgaaa gcgggtagcc acggcgttta gcaataatgc cgcagactat cattaccgca
    18241 agacccatta agatccctgg caaatatccc gccataaaca gcgatgctac agaaacgccg
    18301 ccagctgtca gggaaaacac aattaacacg tttgagggcg gtatcagtaa tccagtaata
    18361 caagatgaga cgtttacagc ggtagaaaat gccggatcat aaccttcttt ggtctgaatg
    18421 ggattcagtg tgccaccaac ggcagcagct gccgcaacgg ctgaaccaga aatggaacca
    18481 aacatcatat tcgccaggac gttcacatga ccaagcgaac caggaacgcg gccaaccatt
    18541 acctgggcga ggttaatcaa acgtatcgca ataccgccgc tgttcatcag tgttccggca
    18601 aagatgaaaa aggggattgc caacagggca aagttatcca gtccattagc cagtcgttgc
    18661 gaaacagtga ttgccgcgat atcccaggga aacatcaaaa gcattgaagc tacagtggca
    18721 ataccgattg agaacgaaat gggcacacca ataaagacca gcacaaaaaa acagccaaac
    18781 agtgtaatcg cgatataact atc
//
LOCUS       DAJTSA010000081        18064 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000081 DAJTSA010000000
VERSION     DAJTSA010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18064
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.081"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            341..1827
                     /locus_tag="OO369_002929"
                     /pseudo
     CDS             341..1827
                     /locus_tag="OO369_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1842..2576
                     /gene="ypdB"
                     /locus_tag="OO369_002930"
     CDS             1842..2576
                     /gene="ypdB"
                     /locus_tag="OO369_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR5983135.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            2589..3446
                     /locus_tag="OO369_002931"
                     /pseudo
     CDS             2589..3446
                     /locus_tag="OO369_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(3449..5944)
                     /gene="ptsP"
                     /locus_tag="OO369_002932"
     CDS             complement(3449..5944)
                     /gene="ptsP"
                     /locus_tag="OO369_002932"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5983136.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(5969..7006)
                     /gene="ypdE"
                     /locus_tag="OO369_002933"
     CDS             complement(5969..7006)
                     /gene="ypdE"
                     /locus_tag="OO369_002933"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5983137.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(7006..8089)
                     /gene="ypdF"
                     /locus_tag="OO369_002934"
                     /pseudo
     CDS             complement(7006..8089)
                     /gene="ypdF"
                     /locus_tag="OO369_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(8104..9351)
                     /locus_tag="OO369_002935"
     CDS             complement(8104..9351)
                     /locus_tag="OO369_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR5983138.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(9373..9699)
                     /locus_tag="OO369_002936"
     CDS             complement(9373..9699)
                     /locus_tag="OO369_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR5983139.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9918..10883)
                     /gene="glk"
                     /locus_tag="OO369_002937"
     CDS             complement(9918..10883)
                     /gene="glk"
                     /locus_tag="OO369_002937"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR5983140.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLCSGAHLRQTLGHIL"
     gene            11087..12343
                     /locus_tag="OO369_002938"
     CDS             11087..12343
                     /locus_tag="OO369_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR5983141.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            12458..12784
                     /locus_tag="OO369_002939"
     CDS             12458..12784
                     /locus_tag="OO369_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5983142.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12925..14163)
                     /locus_tag="OO369_002940"
     CDS             complement(12925..14163)
                     /locus_tag="OO369_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR5983143.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            14499..15701
                     /gene="nupC"
                     /locus_tag="OO369_002941"
     CDS             14499..15701
                     /gene="nupC"
                     /locus_tag="OO369_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR5983144.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15751..17863)
                     /locus_tag="OO369_002942"
                     /pseudo
     CDS             complement(15751..17863)
                     /locus_tag="OO369_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
BASE COUNT         4467 a         4846 c         4467 g         4284 t
ORIGIN      
        1 gagcgacgct gccacaatgg tggctgtttt gaaaatagcc tgattaattt ctgtctgtcc
       61 agcgttttag aacattttta tcaatggatt taaatgagga actccacttc aaaaacagga
      121 aatttttcta cgctaacaag aaaatcacca ccaattttat taacattata atttcaataa
      181 atttaccagc cagaagtgca ttatcaagag atacattgct aatacaccat ttatctttta
      241 atcccctttt aaatttatgg tttttcctca aaaacggcct gctggtcatc agccttcagg
      301 ttacctatca tagaggttta atccttattc agagtcaccc gtgcacgaaa tattcaacat
      361 gctgctggcg gtcttcgatc gggcagcgtt aatgcttatc tgcctgttct ttctcatccg
      421 tatccgcctg tttcgcgaac tgttgcacaa gtcggcgcac tccccaaaag aattgctcgc
      481 cgttaccgcc attttctcgc tgttcgccct gttcagcacc tggtccggcg ttcccgtaga
      541 aggctcgctg gtgaacgtac gtattatcgc ggtgatgtcc ggcgggattc tgtttggccc
      601 gtgggtaggc atcattaccg gcgtgattgc gggtattcac cggtatttaa ttgatatcgg
      661 cggcgtgacg gcgatcccct gctttatcac cagcattctg gcgggttgta tatcaggctg
      721 gataaacctg aaaatcccca aagcacagcg ctggcgctgg atatcgccaa caaaacgcta
      781 ccgctgtttc gccatgtcaa tagcgagtca ttacgcaagg tctgcgaaat tatccgcgat
      841 gacattcacg ccgatgcggt ggcgattact aataccgatc atgtgctggc ctatgttggc
      901 gtgggtgaac ataactatca gaatggcgat gacttcatta gcccgactac ccgtcaggcg
      961 atgaattacg gaaaaatcat cattaaaaac aatgatgaag cccaccgcac accagagatt
     1021 cattccatgc tggtgatccc attgtgggag aaaggggtcg tgaccggaac gctgaaaatt
     1081 tactactgcc acgcgcatca gatcacctcg tcattacagg aaatggcggt cggtctgtcg
     1141 caaatcatct ccacgcaact ggaggtttca cgcgccgagc agctacgtga aatggcaaat
     1201 aaggcagaga ttcgcgccct gcaaagcaaa attaatcccc attttctgtt taacgctctg
     1261 aacgctattt catcgtcaat ccgtctgaat ccggataccg ctcgccagtt gatctttaat
     1321 ctgtcgcgtt atctgcgcta taacattgaa ttaaaagacg atgagcaaat cgatatcaaa
     1381 aaagagctgt atcaaattaa agactatatt gccattgagc aggcccgctt tggcgacaag
     1441 ctgacggtta tctatgatat tgatgaagag gtgaattgct gcattcccag cctgctgatc
     1501 cagccgttgg tggagaacgc cattgtccac ggtattcagc cttgcaaagg taaaggcgtt
     1561 gtcaccatca gcgttgcaga gtgcggaaat cgggtacgca ttgcggtgcg agataccggg
     1621 cacggcatcg ttccaaaggt gattgagcgg gtcgaagcga atgaaatgcc gggcaataaa
     1681 attggcctgc tgaatgtcca tcatcgcgtg aagttattgt atggcgaggg gctgcatatc
     1741 cgccgcctgg agccggggac ggaaattgcg ttttacattc ctaaccaacg caccccagtc
     1801 gcctcacagg ctacgttatt gctttgagcc ggagtgatat tgtgaaagtc atcattgttg
     1861 aagacgaatt cctggcacaa caggaactga gctggctaat taaagagcac agccagatgg
     1921 agattgtcgg cacctttgac gacggtctgg acgtgttgaa gtttttgcag cataaccgcg
     1981 tcgacgccat ttttctggat atcaatattc cgtcgctgga tggcgtgttg ctggcgcaaa
     2041 acatcagcca gttcgcccat aaaccgttta ttgtgttcat caccgcgtgg aaagaacatg
     2101 cggtagaagc gtttgaactg gaggcgtttg actacattct caaaccgtat caggagtcac
     2161 gtattaccgg gatgctgcaa aaactggaag cggcctggca acaacagcag accagcagta
     2221 cgcctgccgc gacggtaacg cgtgagaatg acaccattaa tctggtgaaa gatgagcgaa
     2281 taatcgtcac gccaattaac gatatctatt acgccgaagc gcacgagaaa atgacctttg
     2341 tctatacgcg gcgtgaatcc tacgtaatgc cgatgaacat taccgaattt tgcagcaaac
     2401 tgccgccgtc gcattttttc cgctgccatc gctcattttg tgtcaatctg aacaaaatac
     2461 gcgaaatcga accgtggttt aataacacct acattctgcg actgaaagat ctggattttg
     2521 aagtgccggt cagccgcagc aaagtgaaag aatttcgcca gttaatgcat ctttaatcgg
     2581 gaactttcat gaaagcacca ggcttgcccg ccgatcaaca atttttcgcc gatctgttca
     2641 gcggcctagt gcttaacccg caactactgg ggcgcgtctg gtttgccagc cagcctgcct
     2701 cgttaccggt gggcagttta tgtattgatt ttccccgtct ggatatcgtg ctgcgcggcg
     2761 aatacggcaa cctgctggaa gcaaagcagc agcgaatggt ggaaggagaa atgctgttta
     2821 ttccggcacg cgcggctaat ttaccgatca acaacaaacc ggtgatgctg ttaagcctgg
     2881 tgttcgcacc gacctggctt gggttatcgt tttacgatag ccgcaccacg tcgttgttgc
     2941 atcctgctcg ccagacccag cttcccagcc tgcaacgcgg tgaaggtgaa gcgatgctta
     3001 ccgccctcac ccatcttagc cgttcgccgc tggagcagaa tatcattcag ccactggtgt
     3061 taagtttgct gcatctttgc cgcaacgtgg tgaatatgcc gccgggtaat tcacaaccgc
     3121 gcggcgattt tctctatcac agcatttgta actgggttta ggataattat gcccagccgc
     3181 tcacccgcga gagcgtggcg cagtttttta atatcacgcc caatcatctg tcaaaactgt
     3241 ttgctcagca tggaacgatg cgttttatcg agtatgtgcg ttgggtgcga atggcgaagg
     3301 cgaggatgat tttgcagaaa tatcatctgt caattcatga agtggcacag cgttgcggtt
     3361 ttccggatag cgactatttt tgtcgcgttt tccggcgtca gtttagtctg acgccgggag
     3421 agtacagcgc ccgttttcag ggctaacgtc agaaggttaa ttctgtttcc agcagcgtca
     3481 ggatactttg tgcatcttgc gcggcaaaca acgactggcg gaagttttta ttcaccagtt
     3541 tacgcgccag ctgcgagaag actttcacat gattcatccc ttcgttagca cccagcgtca
     3601 gcatgatcac cagttcgact tcgcccattt ctgactgcca gtcaaccggt ttcgccagcc
     3661 gggcaatgct gatactggaa tgacggatcc actgagattt ggtgtgcggg atcgctacgc
     3721 caaaaccaac gccggtggta acaatctctt cccgctgcca gacatcttct tccagctcaa
     3781 acggatgttc agtgcgcccg ttaacgccga ggttgccgca caggaactgg atcgcctgct
     3841 ctttattgct aaaatcctga tcaacaaaga tattctccag cgccagcagt gggcgaacgt
     3901 cttcttccgg cgtaaaggcg gtgagtaacg cttcaatttc ctgcgcactg cggcattcac
     3961 atgcctgacg cgccagttcc cgacacgcct cgctatccag ttgacgaagc tggcttttca
     4021 ccgccggaat acgcgggcta ctcatactca gctcatccag gcccagccca agcagtagcg
     4081 gcagataacg gctttcaccg cccagttcac cgcaaatgcc tacccatttg ccccgctgat
     4141 gcgcagtggt aactatttgc tgcaacatgc gcaggaacga tggcgtaatc gggttatata
     4201 gcggcgatac gcgcgggtta ttacgatcga ccgcatacag atactgggtc atatcgttgg
     4261 agccgatact gaagaaatcg acctcatcgc agaagtggtc gatgatgtag cacaccgacg
     4321 gcacttccac catgatccca agcgtaatcg tctctgcatg acgcaggcca tcgcgcttaa
     4381 gctcaacgat cgctttttgg atctcgcctt tcacccataa gatctgatcg aggctgtgaa
     4441 ccatcgggat catcaactgg gcgttgccga aactggcggc gcgcaaaatg gcccgcagtt
     4501 gagtgcggaa caggccagca aattccgggt aaatacgtac cgcgcgatag ccgaggaacg
     4561 ggttctcttc ctggggaata ttcagataag gaatgctttt atcgccgccg atgtccatcg
     4621 tgcggaagat aatcggcttg tcgcccgccg ccagtagcac ctgctggtag gcttcaaatt
     4681 gctcctgctc gtccggcgcg ctgtcgcgat ccatatagag catttcggta cggaacagcc
     4741 cgacaccttc cgcgccgttg gcaaacgcgc ctggcgcttc cagagcggtg ccgatattcg
     4801 ccgcaatatc aatacgcttg ttatcacggg aataggccag ctgcgcggca gcctgcgcct
     4861 gttgtttttg gcgtttatcc gccagcgtct gcgcgacctg ataataaccg ctcaccgcgt
     4921 cattcgggtt aatcgccagc acgccgcact gggcgtcaag cactgcaggt tgcccggcat
     4981 aacgggcaat cgcatccagc ggcaagccac tcagtaccgg gatcgccgag gcacgggcca
     5041 gaatcagtgt atgcgaggtg cgcccggttt tctccagaat catacccgca agatttttca
     5101 aatcaaggct caaaaactgg cttggggtta aatcttcagc caccagaatg gtcggttttt
     5161 ccagcaccag attgttgcgc ggcttcagtt ccggccaggt gatatgcagc aactgttcgc
     5221 tgatatcgcg aatgtcgctg acccgctcgc gcagataatc gctggcagaa gcagatagtt
     5281 tggcgcaaac ctgctccata ttgctgatga tcgccgcccc cagtccctga tgctgttctg
     5341 tcatcaggcg acggatattg cctgcaaatt catcatcctg aatcagcgac aaatgagcgc
     5401 tgaggatagt tttgctttcg ccgtcacgct cacgcaattg ctgattcagt tgctcggcaa
     5461 gcgttgccag gctgtgctcc agccgggtgg aatcttgcgc actggcgggg attgcccgat
     5521 aactgtcgag gctgtcgctt tgtaacaggg tcagcgtacc cacgccgacg ccgcttgcca
     5581 gcacattgcc gtacagtaaa tccgggttaa ggcggcttaa tgaacgcggc agcggatgcg
     5641 ccgtcagttc tgcctgcgtc ggctgaacgc tgtcgctgtc gataaagcgc accaggatgt
     5701 actcttccag cacgcgccgc gcctgctctt catcgctgcc gctaatgttc aggctgcaac
     5761 tgtcattaaa taaggtgcca gttccaatca acgccagcga gcttttagca tcagcctttg
     5821 cgttctggcg atggttgata aaggtgattt cgctttgcca ctggctgcac tgttctttaa
     5881 gttcccacgc cggacgagca tgtagaccgt taggcaaagg acagagaaat tgaatcgtta
     5941 acataaccgc tccgggttaa tcaggagatc atctgaaatc cgtcagttga acaaccgtct
     6001 cacgcgtaag acgttgaata agggcagata aaagttgctc catctgcaaa atgtcgcggc
     6061 aatcggcaat cgatgccgcg caatgtccgt ggcgggtggc aggccccatt accaatgtgg
     6121 gtacgccagt accggttaag tgcaccgctc caccgtccgt gccgccgtta ctgaacatat
     6181 ccgcctgcaa cggaacgcca atttctgccg ccacggtttc aatccacgcg gtaagttttg
     6241 gcggcgcaat cagcgattta tcgcttaaca ccagcatcgg cccgttacca atctgacgat
     6301 ggttagccgc gccataatca aagtttttcg cccagcaggc ggtatcaagc acaatggcga
     6361 cgtccggcga caccgcgcgg gtagcagttt gcccgccgcg taatcccacc tcttcgctgg
     6421 aacttgccac cagccacact tccgcaggca gttcggcgtc gtgcagctca cgtagtaacg
     6481 tcaccagcag atagcaactg aggcgatcat caaaggcttt gcccatcacc cgctggtgag
     6541 ggaggacctg aaaagtggta tcgaaggtga cgcgatcgcc tggacgaatt cccgcctgca
     6601 tcacttcgtc ataggtgcgc gcgccaatgt ccacgcgcat ggcgctaacg tcattcccct
     6661 gccggtcgcc gtcaagcagg cctggaattt tgcactcttc acgggtggtg atgcgcaccg
     6721 gctgcagctg gcgggcagcc atgcgcacgt tgccaaccgg cagcacgtca atagcgcctt
     6781 cgcgggagat gctgcgcacc ataaatccca cttcgtccat atgggcgcag atcatcacct
     6841 tcggacctgt cgattcattg aggcggatca gcaccgatcc cagaccatca aatcgcactt
     6901 ctttttgcag gcgagccgct tcttccagca ggatctgccg cacttcctgt tccgaggagg
     6961 cgatcgcatc tgcctcgctc aacgctttta atagcgataa atccattatg cctctcccgt
     7021 gagcaacact gttttcggca tggcgtagag cacttctgcg ccttgcgggg taaccagcac
     7081 aacatcttcg atgcgcacgc ccccttgccc tagcaaataa atccccggct ccacggtcag
     7141 caacatgcct ggctgtagcg tcgtggtgtc ccgcggtgaa aaacgcggat cttcatggac
     7201 ttcaatgccg atagcgtgac cagtgttatg accgaaatag tcgccaaatc cagcctcggt
     7261 aatgacccgg cgcgcggcgt cgtcaatctg ctggcagcgc acgccggggc gaattgcaga
     7321 gattgccgcg agctgtgcct gcaggacaat ctgatagacg tcaaacagtg ggtgagattc
     7381 ggcgctcacc ccttcgccat tcaccagcaa ggtgcgcgtc atatcagcag tagccctgat
     7441 acagcgcacc aaaatcgaga gtgacaaact cgcccgctgc aacaatcttg tcgctggctt
     7501 tgccgtgcgg cagcgccccg cgccagccgc tggcgacaat ggtgtcaaaa gaggcttttt
     7561 ctgcgccctg ctggcgcata aaccactcca gttcagcggc tatctcgcgc tcgctcatcc
     7621 ccgcctgaat aaagcggcga atatgctccg ctccgcgatc ggcaatccca caggcgaggc
     7681 ggattatctc cacctcctct ggcgttttga tttgccgcag cacatccggc gtggcgctaa
     7741 ccagtttcgc attgagttca gacttccagc ggtgcgcggt ttcccaactc acctgctggc
     7801 cttcaaaacc gagcgtttgc aactgctcat cggcaatgat ttgattgacg atagtggtaa
     7861 gcgtgttcgt tgcgtcaagc aaatgcagct ggtagccttg cgcacgggct tccacctcaa
     7921 cgaaatagcg cgaatctacc agaatgtgcg cactttcacg gctaataacc acatagcctg
     7981 atccggtgga gatccccaga tgcggctgtt tgttctgccg tgaggagaga agcaccgcat
     8041 ccagttgttg caccttaagc cagttgcgca gcgaagcgag taatgtcatc tggattttcc
     8101 ttcttacagg ctatcgatta acaatttgcc tttgcggaac atcatcagac gcaggaacac
     8161 caccatcagc gcagtaatga ttgcccccag cgcgatcccc gccatataaa cgcccaagtt
     8221 agtgaccagc ggccatgccc agatagcgga ttccgggaac cattgcactg cgcccagcca
     8281 gacagctgcg gttgagccga caatcgcgcc gaccatatat gacggaatag cggtgatggg
     8341 gctttccagc gcaaacggaa tcgcaccctc actaatcccc ataaaggcca ggaacatcgc
     8401 ggtcttaccc tgcggataaa gttgagcgtt gaacaggcgt ttgccggtta aacgacggtc
     8461 aataatggtc gccagaccca gaccaatcgg cggaataacg atagcgatag aacgagcggt
     8521 gaccggtaat acgtggtcag tggtaaagct gaaggcaacg aaacctgcgg ctttgttgat
     8581 cgggccacca aggtcgattg ctgtcgccgc ggcgatcccc attgcgtaca tcagcgcgcc
     8641 cttctcacct gcggcagtca gcacagtacg gataccgccg ttgatccagc caccaaaggg
     8701 agtgatgacg tagtacatcg ccagcatgac aaaaatggcg gaaagaatcg gtagcaaaaa
     8761 tgtggttttg aacgctaaca ggaaatccgg taactggatt ttctggttca tccatttcac
     8821 aaggtaaccg gcaacgatag aaataatcag cgcaccaatg aaggtggacg gcaccggcga
     8881 agaggtcgcc cattgcatag tgctgggatc gaagttcagc agttgcgtcg gctgggtaga
     8941 catcaacccg ccgataaaac cagccgggaa cgccagtttg ccaccgatag agttcgccac
     9001 aaatgcagca aacatcggaa tggcaaaacc aaacaacacg ccgccgaagg actgcgacag
     9061 ccaggcaaat ttaagtaagg agaggtcgaa accggagaat ttcccgctat taagagcatc
     9121 catgatgccg atctcagcag gaattttaag ccagctataa gcaatcagct ggctgaaagc
     9181 gaggatcaca ccgcccataa ttaaggtcgg caccatgcgt gaaatccctg acatcacatg
     9241 ctgcgggagt tcaccccaga agctgctttt agaggcctgc ggattcttaa ctgccgctgc
     9301 cgcaccggat gcgccaggca caacggttgc actgcgtttt ttaatggcca taacaattat
     9361 ccctgtcgaa tattactgtt gttcagaggc aatcatctct tcgatttctt tgatgatgcc
     9421 cgcagcgttt ttaattgcgt cctgcaaagt gatttcataa acgtcgcgtg attcgaaacg
     9481 ttcgttatct tccggggtaa ctgccacgga atggatgatg atggtcgctt cggcgatatc
     9541 ctgcgccgtc aggcgattct ggatgccgtc cgcgccctgg gtttcaattt tcacttcata
     9601 accggcttct accgccgctt cttccagcac ctgagcggcc ataaaagtgt gagccaggcc
     9661 cattgggcag gcacataagg caatcagttt cttactcatc gttaattcct cgttcagtga
     9721 tagttgacta gtgaaattat gaacgtgcaa aacaaatcgc cgttaccaga caaataatgc
     9781 atttactgga gaattaatcc cgctgtacgg aagtgtgacg caggtcggcc ttgtgagggg
     9841 acacaaagaa aaattgaatt aactgtatga tttaaaagat tatcgggaga gttacctccc
     9901 gatataacag gaaggattta cagaatgtga cctaaggtct ggcgtaaatg tgcaccggaa
     9961 cagagaagac ccggatagtc atggacgatg agatacaccg gaatatcatg gacatattct
    10021 ttaaagcgcc ctttatcttc aaatgcggca cggaaaccgg aggctttgaa gaactcaagg
    10081 aagcgcggca cgataccgcc cgcaataaac acgccgccaa atgtcccgag attgagcgcc
    10141 agattgccgc caaaacggcc cataatgacg caaaacagcg acaatgcgcg gcggcaatcg
    10201 gtgcagctgt cagccagcgc gcgttcggta atatcttttg gcttgagatt ttctggcagg
    10261 cggttgtcag ctttcacaat tgcgcgatac aaattcacca gcccagggcc agaaagcacg
    10321 cgctccgccg aaacatgacc aatttccgca cgcaatattt cgaggataat ggcctcttct
    10381 tcactattcg gcgcaaaatc aacgtgaccg ccttcgcccg gcaagcttac ccaacgctta
    10441 tcgacatgga ccagatgcgc aaccccaagc cccgttccgg caccgtaaac cgcaataggc
    10501 ttaccttcga ccggttctgc gccaccaaac tgaatcagat gctctttttt cagcatcggg
    10561 atcgccatcg atacagcggt aaaatcgtta ataatttcca gatggctaaa accgagattc
    10621 tttttcattt cggcaattga gaacgcccag gtatggttgg tcattgccac ccagtcaccg
    10681 gtaattgggc aagcgatggc aatacagccg tctttcacct cgaccttatg ttcttcaaga
    10741 taaacgcgaa tgaccgcttc gaggctgggg taatcaagcc ctgaataggt cttagcctgc
    10801 gagatttcac cactggcaat atcacacaga gcaagacgtg cgttggtgcc gcccacatca
    10861 ccgactaatg catactttgt cattcttcaa ctgctccgct aaagtcaaaa taattctttc
    10921 tcacactgta aatacctggg ggcataacaa caacgccgga aaggcaggct ccctgtaaat
    10981 atcgatctgg gtcacacaat tactttatcg tttcagcacc aattgcagcg atgccttttt
    11041 gcaagctggg caaactaagt atctgacccc gcataaggaa tagaacatgc tccatccgcg
    11101 agccagaacc atgttgttat tatcgctccc cgccgtggca attgggattg cgtccagtct
    11161 tattctgatt gtggtgatga aaatcgcctc ggtattacag aatttgctct ggcaacgact
    11221 gccgggaact ctggggatag cccaggattc acccctctgg atcatcggtg tattaacgct
    11281 aacgggtatt gcggtggggt tggttatccg tttcagccag ggtcatgccg gaccagaccc
    11341 cgcctgtgaa ccgctgatcg gcgcaccggt tccgccctct gcgctaccta gacttatcgt
    11401 agcattaatt ctcggtcttg ctggcggcgt cagcctgggg ccggaacatc cgatcatgac
    11461 cgtcaatatc gcccttgccg tcgccattgg cgctcgtctg ttaccgcgcg tcaaccgaat
    11521 ggagtggact attttagcct ctgccggaac catcggcgcg ctgtttggta cacctgttgc
    11581 ggcggcgttg atattttcgc aaaccttaaa tggcagtagt gaagttccgc tatgggatcg
    11641 tctttttgcg ccgttaatgg cggcagcagc tggtgcactt actaccggat tatttttcca
    11701 tccccatttt tcactgccca ttgcgcatta cggacagatg gaaatgaccg atattctcag
    11761 cggtgcaatt gtcgcggcga ttgccatcgc agcagggatg gtcgccgtat ggtgcttacc
    11821 acggttgcac gcgatgatga atcaaatgaa aaatccggtg ctcgtgctgg gtattggcgg
    11881 atttattctc ggaattctgg gggttattgg tggaccagtt tcgctgttta aagggctgga
    11941 tgagatgcag cagatggtgg caaatcaggc tttcagcacc agcgattact ttttgctggc
    12001 ggtaattaaa cttgccgccc tggtcgttgc tgccgccagt ggctttcgcg gtgggcgaat
    12061 cttcccggca gtgtttgtcg gcgtggcatt agggttgatg ctgcatgagc acgttcccgc
    12121 cgttccagcg gcaataaccg tttcctgcgc tattctcggc atcgtgctgg tggtaacacg
    12181 cgatggctgg ttaagtcttt ttatggcggc agtcgttgta cccaatacca cattgctacc
    12241 gctgctctgt atcgtcatgc ttccggcatg gctgttatta gcaggtaagc cgatgatgat
    12301 ggtcaatcgt ccgaagcaac agccacccca cgataatgtt tagcaaaaaa tgctctcctt
    12361 tatgatttaa gagttatggc gatgatacgg ggctttacct ccccgtaata ttgcgttaac
    12421 aggccgctga cggcttcgat cgtgaaggag aataacaatg ttcaggtcac tgtttctggc
    12481 ggccgccctg atggcattta ccccgcttgc agcaaatgca ggtgaaatca ccctactgcc
    12541 atcgatcaaa ttacaaattg gcgatcgcga tcattacggt aattactggg acggtggtca
    12601 ctggcgagat cgtgattact ggcatcgcaa ttatgaatgg cgcaaaaacc gctggtggcg
    12661 tcatgataat ggctatcatc gtggctggga taagcgtaaa gcatatgagc gtggctatcg
    12721 tgaaggctgg cgcgatcgtg acgatcatcg cggaaaaggc cgcggacatg ggcaccgcca
    12781 ttaaacgcgt cctccaatgg agcacaatgc ctgatgcgac gctagcgcgt cttatcaggc
    12841 ctacaaaccg gctctttaac gcgtagggcg gataaggcgt ttacgccgca tccgccaata
    12901 gtgccagatg cgacgctcat tcaactacaa tcccagcgcc gtccccacca acaaccagat
    12961 attcagcgcc acgaccagca ctacaatcac ccagcctgtc tgttttacgc gtttgctgtt
    13021 caccagatcg cccatcaact tgctgtcact ggtgaaaatc agcagtggaa ccagcgccag
    13081 ggcgatacca aaacttaaca gcacctgact cataaccaga atccgtgtcg gatctaatcc
    13141 catcagaatg acaataaatg acggcaacat ggtgactgta cgacgcaccc acagcgggat
    13201 atggaagcga atgaatccct gcatcaccac ctgccccgcc agtgtcccca ccaccgttga
    13261 ggacagtccg gcagcaacca gacttaaccc aaagaccgtt gccgcagcat ggcttaacag
    13321 cggttgcagc gtcagataag cctcatcaag atcggcaaca ccagtatgac cagaaaagtg
    13381 gaacgccgcc gcagctgtag ccatcatcac cagattgaca aaaccggcaa tcgtcatggc
    13441 gatagccaca tcccatttgg tggcggaata acgttgttga cgcgaaccgc catgtaaatg
    13501 ctgagtgagc gaagagtgca aataaatcac atgcggcata atcgtcgccc ctaacacgcc
    13561 tgctgccagg aagaccgcct ccgaagtagg taaactcggg atcaccattc ctttacccag
    13621 ctgcgccagg ttaggctggg agaaaatcaa ctcgacaatg taagccgcgg caacaaacaa
    13681 cagtaacccg ccaatcactt tctccagcgg tttttgcccg cgacgttgca gcattaaaat
    13741 caggaaagtc gcgatccccg tcagcaccgc gccctgcaac aacgaaacac caagaatgag
    13801 tttaaaaccg atcgccgcac caataaattc cgccaggtcg gttgccatcg caataatttc
    13861 tgcctgaacc caatagaacc acactacggg acgcggatag tgatcacgaa tctgctccgc
    13921 cagattttta ccggtggcaa tccctagttt ggcagagagg atctgaatca gcatcgccat
    13981 caggttggcc caaacgacaa cccacagtag ctgatagccg aagctagcac ccgcctgaat
    14041 attggtcgca aagttaccgg gatcgatata accaatcgcc gcaatgaacg caggtcccat
    14101 taatgcgagc ctcatcttac gcgccgcccg tccgctgcta ctctcaacgc gatagttcgt
    14161 catcttgtgc ctctaaaaca tagcctttgc tatgtttcat gctatgctaa acgagaatga
    14221 ttatcaaatt catttaaatg gattgtggtg atttctctga tagaccaaga ttatgattac
    14281 gaaaagattg atgacttgaa tgttatggtt atgtttaatg ttagcacatt tacataactt
    14341 tcagcttcca tacacaacat agcagaaatg tatgacagat cactattttt gaagcctgtc
    14401 acaggacgtc attatagtgt gtgtcagatc tcgttttcct taaccatgtt acatagaatg
    14461 tgcacggaaa tttaacctgc ctcatatttg gagcaaatat ggaccgcgtc cttcattttg
    14521 tactggcact tgccgttgtt gtgattctcg cactgctggt aagcagcgac cgcaaaaaaa
    14581 ttcgtatccg ttatgttatt caactgcttg ttatcgaagt gttactggcg tggttcttcc
    14641 tgaactccga cgttggttta ggcttcgtga aaggcttctc cgaaatgttc gaaaaactgc
    14701 tcggatttgc caacgaaggg actaacttcg tctttggtag catgaatgat caaggcctgg
    14761 cattcttctt cctgaaagtg ctgtgcccaa tcgtctttat ctctgcactg atcggtattc
    14821 tccagcacat tcgcgtgttg ccggtgatta tccgcgcaat tggtttcctg ctctccaaag
    14881 tcaacggtat gggcaaactg gaatccttta acgccgtcag ctccctgatt ctgggtcagt
    14941 cagaaaactt tattgcctat aaagatatcc tcggcaaaat ctcccgtaat cgtatgtaca
    15001 ccatggctgc cacggcaatg tccaccgtgt cgatgtccat cgttggtgca tacatgacca
    15061 tgctggaacc gaaatacgtc gttgctgcgc tggtactgaa catgttcagc acctttatcg
    15121 tgctgtcgct gatcaatcct taccgtgttg atgccagtga agaaaacatc cagatgtcca
    15181 acctgcacga aggtcagagc ttcttcgaaa tgctgggtga atacattctg gcaggtttca
    15241 aagttgccat tatcgttgcc gcgatgctga tcggctttat cgccctgatc gccgcgctga
    15301 acgcactgtt tgccaccgtt actggctggt ttggctacag catctccttc cagggcatcc
    15361 tgggctacat cttctatccg attgcatggg tgatgggtgt tccttccagt gaagcactgc
    15421 aagtgggcag tatcatggcg accaaactgg tttccaacga gttcgttgcg atgatggatc
    15481 tgcagaaaat tgcttccacg ctctctccgc gtgctgaagg catcatctct gtgttcctag
    15541 tttccttcgc taacttctct tcaatcggga ttatcgcagg tgcagttaaa ggcctgaatg
    15601 aagagcaagg taacgtggtt tctcgcttcg gtctgaaact ggtttacggc tctaccctgg
    15661 tgagtgtgct gtctgcgtca atcgcagcac tggtgctgta aaaccataca taaaaaagcc
    15721 ggggatcatt ccccggcttt tttacgcctg ttaatcagct aatggctgcg ggcgaccaat
    15781 caaataccct tgcagatatt gcaccccgag cttatgcaat agcgcctgct gctgttgcgt
    15841 ctcgacaaac tccgcgacca cactcaatga cttcgctttc gccagatcgg taattgatct
    15901 cacaatcatc gcatccagcg tattcgtgac aatatctttc acaaagacgc catcaatttt
    15961 gatgatatca gcctgcaaac gctttaaccg ttcgtagttg gcatatccgg tgccaaagtc
    16021 atcaatcgca atccggaaac caaacttatg cagctgctcg atgttgtaca tgctgctttc
    16081 tgcgttagaa aacgcctgct cctcggtgat ctcaagaatg accgcctgcg gggagatgtg
    16141 ataacgctta aacagacgaa taatccgccc ggcaatattc ttttgcagca gcgtgagcgg
    16201 cattaaattg actgaaaagc gcggtccttt tttgtcgcaa gggtgtgttg ccagccactt
    16261 caacaaggat tccagcactt gcaaatcaaa acgcgcgcta aggttaaact gagcaataag
    16321 gggcagaaac ttatccgggg tcataatgcc gccgtcatat ttcagtcgcg cgaggatctc
    16381 atcataacct tcaccctctt tgttgcgaat tggctgggcg tagagcagca aatcaccctg
    16441 atctaacgcc gtgcgaatgg tattcagcaa tagcacctgt ttagtggtct gcccggaaac
    16501 catctcctct ctgctatcca gcgccagcac atgatgatgt gcgcaggatt gctccgccag
    16561 ccagcttaac tgccccaaca agggttgcag ggtttcctga tttccatcaa aacgccccca
    16621 ggcagcacca tagcccatat ccagcccggt attgttccag tgaatttgcc gactattcag
    16681 gatgttaacc atatgctgga gtcgcccttc cgtttccggc ccgctcagca ctaacagcag
    16741 ttcactaccc ggcaattgat acaacttttc gttttcctgc atcaacggca gcagcgtacg
    16801 gcaaattgag cggatacagt gaacgcgcat cattaagccg taatgacgac tcataaactc
    16861 aagattatca atgcgcaggc agcaaaaact cttgcccgcc tcttgctccg gcgcttgttc
    16921 caacgcacga aagttgggta ggagcgttaa cggatctgtt agcgcctgca aatgccagcg
    16981 gcgattaagc cattcactgc gatgataaat gcgcaccata tagagcaggc aaacgctaaa
    17041 ggaaatcagc accgcgagaa taaatgccag cgaatattcg gtttccaccc cttgcaaaaa
    17101 gttctgattg taattcagaa ggcaaagcgt tgaaacagcc caggtgagat ttaaaaacgg
    17161 atagcgaaga ttaccgaccc cgagggtaaa gatgatgaag aaaacgggta ccaggtaacc
    17221 ggcaataaag tcgttttcat aaggtgtgca caacagaagt agcagcacgc ttaacgctgc
    17281 cagccagctt aaggtaaatg cgagtgagat agtagaaaag catgttgtaa atcagcactg
    17341 cggtgaaaag gcttagtaaa tcaacgaccg tgaaaatggc atccgcatcg ccgaaaaagg
    17401 tggatatctt tagcgggaaa tcaaagaagc tccccacaag atacatgctg catttgatgc
    17461 cgatcggcgt caccaaacca agccagaaca gacgttgcca gacatatcgg ctggtcaatc
    17521 cataacgcca acgcgtcccc agctgccagc gtaatattgc gcaggcacac aacacgacaa
    17581 acgtctggct gccgagcatg accgcagtct gtaaaaaaga gagatgaaag ttccacagat
    17641 tggtgcaaaa catcccgcat aaaacgggaa cgacaccgcg ccagccaaag ataaacaaga
    17701 ttgacagcat tacgcacagt ggcatccacg ccagaaagat ataactggaa tggataatgg
    17761 caagtggcga aataaaacgg gatagctgaa taagtaccac ggtgagcgta aacgctagtg
    17821 tgaatatctt gatatttttt atcaggttat gctccacaaa catgagtaca ctactctcac
    17881 tgatcggggt tttcaggcac acaaataata agtttattcg catcgcgaag caaacagggg
    17941 ccagcctgtt gccgattttt catccaatga tgctttcacc aggcggcgct aacccggacg
    18001 tagactgtat tacaaaagcg gcaaaaagca gagacaaaaa acccccgctt tgcagcgagg
    18061 gttg
//
LOCUS       DAJTSA010000082        18045 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000082 DAJTSA010000000
VERSION     DAJTSA010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18045
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.082"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            441..1124
                     /locus_tag="OO369_002943"
     CDS             441..1124
                     /locus_tag="OO369_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5983145.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGLSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            1168..5830
                     /locus_tag="OO369_002944"
                     /pseudo
     CDS             1168..5830
                     /locus_tag="OO369_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            6198..7853
                     /gene="lldP"
                     /locus_tag="OO369_002945"
     CDS             6198..7853
                     /gene="lldP"
                     /locus_tag="OO369_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR5983146.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7853..8629
                     /gene="lldR"
                     /locus_tag="OO369_002946"
     CDS             7853..8629
                     /gene="lldR"
                     /locus_tag="OO369_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR5983147.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8626..9816
                     /gene="lldD"
                     /locus_tag="OO369_002947"
     CDS             8626..9816
                     /gene="lldD"
                     /locus_tag="OO369_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR5983148.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            10012..10485
                     /gene="trmL"
                     /locus_tag="OO369_002948"
     CDS             10012..10485
                     /gene="trmL"
                     /locus_tag="OO369_002948"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR5983149.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10538..11359)
                     /gene="cysE"
                     /locus_tag="OO369_002949"
     CDS             complement(10538..11359)
                     /gene="cysE"
                     /locus_tag="OO369_002949"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR5983150.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11439..12458)
                     /gene="gpsA"
                     /locus_tag="OO369_002950"
     CDS             complement(11439..12458)
                     /gene="gpsA"
                     /locus_tag="OO369_002950"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR5983151.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12458..12925)
                     /gene="secB"
                     /locus_tag="OO369_002951"
     CDS             complement(12458..12925)
                     /gene="secB"
                     /locus_tag="OO369_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR5983152.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12988..13239)
                     /gene="grxC"
                     /locus_tag="OO369_002952"
     CDS             complement(12988..13239)
                     /gene="grxC"
                     /locus_tag="OO369_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR5983153.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13381..13812)
                     /locus_tag="OO369_002953"
     CDS             complement(13381..13812)
                     /locus_tag="OO369_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR5983154.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            14057..15601
                     /gene="gpmM"
                     /locus_tag="OO369_002954"
                     /gene_synonym="pgmI"
     CDS             14057..15601
                     /gene="gpmM"
                     /locus_tag="OO369_002954"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR5983155.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15611..16894
                     /gene="envC"
                     /locus_tag="OO369_002955"
     CDS             15611..16894
                     /gene="envC"
                     /locus_tag="OO369_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR5983156.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16898..17857
                     /locus_tag="OO369_002956"
     CDS             16898..17857
                     /locus_tag="OO369_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR5983157.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17844..18045)
                     /locus_tag="OO369_002957"
                     /pseudo
     CDS             complement(17844..18045)
                     /locus_tag="OO369_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4545 a         4662 c         4673 g         4165 t
ORIGIN      
        1 actcccttta cttcgattta ccgctggttg atattctgaa aatgcatttt taccgaaata
       61 gccatctata aacaataaaa atatatagaa gatatcattt gttaattaac aagcatttac
      121 gtctcttttt ttcggattta attgtgaaat tttaatcatc atattcacaa aatcaattat
      181 ctgtgaaatt tttcccagac atggtttctt tcatatttga catttattaa taatcatcat
      241 tagaatccac gaaaaataat atattcatac aaacacagac atcttttgaa acgaatgata
      301 catttttatg atgaatgctt aaaacaatat ttaatcgaca cacatcactg tcattaatgt
      361 aaagcaacat agctcaccaa taatacaatg gacaactcta ccggcattga gcgctggtcg
      421 gaggaaataa ggatttttac atgaacttga agaagatttt tttcagtgct gtcactgttt
      481 cagttctctg cgcattaacg ggatgtgact acattgaaga aggcaagcca gaaagtagtt
      541 tactgaaaca acaagaagaa cataataata aaattgtgct acttgaaaaa caacaagcac
      601 agctaaagag tcaactcgaa actattcaaa aacaacaaac tggcataata aacagtacaa
      661 agactttaac tcacgtaatt aaatccgtta aagaccaaca aaatactttt atttttaccg
      721 agtttaaccc agcaaaaacg aagtatttca tcctgaacaa cggttcggtg gctttagcgg
      781 gtcgagtgtt atccattgac gccacagaga atggtagtgt tattcatatc tcactggtca
      841 acttattaag tacacctatc tcaaacattg gttttaatgc gacatggggt ggtgaaaaac
      901 ctgtcgatgc caaagagttt gcccggtggc aacaattact tttcaacaca tcaatgaaat
      961 ccacattgaa attattacca ggtcaatggc aagacattaa tttgaccctg aagggtttat
     1021 cgcccaataa cctgggatat ctgaagttag ccatcaacat ggaaaatatt cagttcgaca
     1081 atcttccctc tgctgaaaat cggcagaaaa gaagcaaaaa ataaatgcag ttagtacgct
     1141 tataacaagg aatagcgagc atcattaatg aacaaaatat ttaaagttat ctggaaccct
     1201 gcgacaggga attatactgt taccagcgaa acggcaaaaa gccgtggcaa gaaatctggg
     1261 cgcagtaagc tgttaatttc tgcgctggtt gcgggtggaa tgttgtcgtc gtttggggca
     1321 ttggcgaatg ccgggaatga caacggtcag ggtgttgatt acggtagtgg atcagctggc
     1381 gacggctggg ttgctatagg caaaggggcg aaagcaaata cttttatgaa caccagtggt
     1441 tccagtactg ctgtgggtta tgacgctata gctgaaggcc aatatagctc tgccatcggg
     1501 tcaaaaaccc atgcgattgg tggtgcatca atggcctttg gggttagtgc aatatcagaa
     1561 ggcgatagaa gtatagcact gggtgcctct tcgtattcat tgggccaata ctcaatggcc
     1621 ctcggccgtt attcaaaagc attgggtaaa ttgtctattg ctatggggga ctcttccaaa
     1681 gcggaaggag caaacgccat tgccctgggg cgtagcagtg tagctagcgg tactgacagc
     1741 ctcgcatttg gcagacaatc acttgccagc gcagcgaacg ctattgcgat aggtgctgag
     1801 accgaagccg ctgaaaatgc aactgctatt ggcaataatg cgaaggcaaa agggactaat
     1861 agcatggcaa tggggttcgg aagccttgcc gataaagtca atactatcgc attaggaaat
     1921 ggcagccagg ctctggcaga taatgcaatc gccataggcc agggcaacaa agctgatggc
     1981 gtggatgcca tcgctctggg taatggtagc cagtcgagag gcttaaacac cattgcctta
     2041 ggcacagcca gtaatgcaac tggtgataag agtcttgcgc ttggtagtaa tagcagtgcc
     2101 aacggtatta actctgtcgc gctgggcgca gattccattg cggatttaga caataccgtc
     2161 tctgtcggca atagttcatt aaaacgcaag atcgttaatg tgaaaaatgg cgcgatcaag
     2221 tctgacagtt acgatgccat taatggttca cagctttatg ccattagcga ctcggtagca
     2281 aaaaaggctt ggaggagggg ctgcagtaga tgttgatgac ggtactgtta cagcaccaac
     2341 ctacaattta aaaaatggta gcaaaaataa cgtaggggct gcgctcgctg tacttgatga
     2401 aaacaccctg caatgggacc aaaccaaagg caaatacagc gctgctcatg gtactagtag
     2461 cccaactgcc agcgtaatca ccgatgttgc ggatggcacg atttcagcct ccagtaagga
     2521 tgcggttaac ggttcccaac tgaaagctac caatgacgat gtcgaagcca acaccaccaa
     2581 tatcaccaac ctgacggatt ccgttggtga ccttcaggct gatgccctgc tctggaacga
     2641 aactaaaaag gcattcagtg cagctcacgg ccaggatacc accagcaaaa tcaccaacgt
     2701 taaagatgcc gacctgacgg ctgacagcac tgatgctgtt aacggctctc agctgaaaac
     2761 caccaacgat gctgtggcga cgaataccac caatatcgcc aataacactt ccaatattgc
     2821 cactaacacc accaacatct ctaacctgac tgagacggtg actaatcttg gtgaggatgc
     2881 gctgaaatgg gataaggaca atggtgtatt cacggcagct catggcaccg agaccaccag
     2941 caaaataacc aacgttaaag atggcgacct gacgactggc agcaccgatg ccgttaacgg
     3001 ctctcagctg aaaaccacca acgatgccgt ggcgacgaat accaccaata tcgccactaa
     3061 caccaccaac atctctaatc tgactgagac ggtgactaat cttggtgagg atgcgctgaa
     3121 atgggataag gacaatggtg tcttcactgc agctcatggc aacaataccg ccagcaaaat
     3181 caccaatatc ctggacggca cagtcactgc aaccagttcc gatgccatta acggtagcca
     3241 gctttatgac ttaagcagca atatcgccac ctacttcggc ggcaatgctt ctgtgaatac
     3301 tgacggtgtg tttaccggtc caacctacaa aatcggtgaa acaaattatt ataacgtcgg
     3361 cgatgcactg gctgcgatta actcctcatt tagcacgtct ctcggcgatg ctctgctttg
     3421 ggatgccacc gcaggtaaat tcagtgccaa acacggtact aatggtgacg caagcgtgat
     3481 cactgatgtc gcagatggtg aaatttcaga ctccagttct gacgcagtaa acggctcaca
     3541 actccacggc gtgagcagtt atgttgttga tgcgctgggg ggtggtgccg aagtcaatgc
     3601 agacggcacc atcactgcgc cgacgtacac cattgctaat gctgattacg ataatgtcgg
     3661 tgatgccctg aatgctatcg ataccactcc tgacgacgct ctgctctggg atgcggacgc
     3721 cggtgaaaat ggtgcattta gcgccgctca cggaaaagat aaaactgcca gtgtaatcac
     3781 taacgtcgct aacggtgcaa tctctgctgc cagcagcgac gcgattaacg gctcacaact
     3841 ctataccacc aataagtaca tcgctgatgc gctgggtggt gacgcagaag tcaacgctga
     3901 cggcaccatc accgcaccga cttacaccat tgcgaacgcc gagtacaaca acgtcggtga
     3961 cgccctggat gcgcttgatg ataacgccct gctgtgggat aagactgcca atggcggtgc
     4021 tggagcctac aatgccagcc atgacggtaa agccagcatc atcactaatg tcgctaatgg
     4081 cagtattagt gaggacagta ccgatgcagt gaacggttct cagttgaatg cgacgaatat
     4141 gatgattgag cagaacaccc aaattatcaa tcagctcgct ggtaacaccg acgcaaccta
     4201 tatcgaagaa aatggcgctg gtatcaacta cgttcgtact aacgacaacg atttagcctt
     4261 taacgatgca agcgcctctg gtgtcggcgc tacagctgta ggttataacg ctgtcgcgtc
     4321 tggtgccagc agcgtagcca ttggtcagaa cagcagcagc accgttgata ccggtattgc
     4381 gctgggtagc agctccgttt ccagccgtgt gattgccaaa ggttctcgtg acactagcgt
     4441 aacggaaaat ggcgtggtga ttggttatga caccactgac ggcgaactgc taggtgcatt
     4501 gtcaattggt gatgacggta aataccgcca aatcatcaac gtagctgatg gttcagaagc
     4561 ccatgacgcc gttacggttc gccagttgca gaacgctatt ggagcggtcg ccactacgcc
     4621 aaccaagtac tttcacgcca actcaacggc agaagactca ctggccgttg gtgaagactc
     4681 gctggcaatg ggtgcgaaaa ctgtcgttaa tggtaatgca ggtattggta tcggtttgaa
     4741 cactctggtt ctggctgatg cgatcaacgg cattgctatc ggcagtaacg cacgcgcaaa
     4801 ccatgcaaac agtatcgcaa tgggtaacgg ttctcagacc acccgtggtg cacagactgg
     4861 ctacaccgcc tacaacatgg acgcaccgca gaactctgta ggtgagtttt ctgtcggtag
     4921 tgaagacggt caacgtcaga tcacaaacgt cgcagctggt tcagcggata ccgatgcggt
     4981 taacgtgggt cagttgaaag tcactgatga gcgcgtagcg caaaataccc agagcattac
     5041 taacctgaac aatcaggtca ctaatctgga tactcgcgtt actaatatcg aaaacggtat
     5101 tggcgacatt gtcaccaccg gtagcaccaa gtacttcaag accaacaccg atggcgtaga
     5161 tgccaacgcc cagggtaaag atagcgttgc tattggttct ggttccattg ctgccgctga
     5221 caacagcgtc gcactgggta ccggttccgt tgcagaggaa gaaaatacaa tctctgtagg
     5281 ttcttctact aaccaacgcc gtatcaccaa cgtagctgcc agtgttaatg ccaccgatgc
     5341 ggttaacgtg tcgcagctga aatcttctga agcaggcgga gtgcgctacg acaccaaagc
     5401 tgatggttct atcgactata gcaatatcac cctcggtggt ggcaacggca gtacgactcg
     5461 tatcagcaac gtctccgctg gcgtcaacaa caacgacgcg gtgaactacg cgcagttgaa
     5521 gcaaagcgcg caggaaacga agcaatacac cgatcagcgg atggttgaga tggataacaa
     5581 actgtctaaa actgaaagca agttgagcgg tggtatcgct tctgcaatgg caatgaccgg
     5641 tctgccgcag gcttatacac cgggtgccag catggcttct attggtggcg gtacttacaa
     5701 cggtgaatcg gcagttgctt taggtgtatc gatggtgagc gccaatggtc gttgggtcta
     5761 caaattacaa ggtagtacca atagccaggg tgaatactcc gccgcactcg gtgccggtat
     5821 tcagtggtaa tgatccatta agttagtgtg actaagggca aacgccaaat tgcctgatgc
     5881 gttacgcgca tcaggcaaga taaggcgctc cgggtcaaca atctaagggc aattctctga
     5941 tgaggattgc ccttttcttt accagacatc tccccccaca agaattggcc ctaccaattc
     6001 ttcgcttatc tgacctctgg ttcacaattc cccaattaaa actcacatca atattgccaa
     6061 tacataacat ttagttaacc attcattgtc attatcccta cacaacacaa ttggcagtgc
     6121 cacttttaca caacgtgtga caaggagatg agcaacagac tcattacacg atgtgcgtgg
     6181 actctaggag acctgcaatg aatctctggc aacaaaacta cgatcccgcc gggaatatct
     6241 ggctttccag tctgatagca tcgcttccca tcctgttttt cttctttgcg ctgattaagc
     6301 tcaaactgaa aggatacgtc gccgcctcat ggacggtagc aatcgccctt gccgtggctt
     6361 tgctgttcta taaaatgccg gtcgctaacg cgctggcctc ggtggtttat ggcttcttct
     6421 atggtttgtg gcccatcgcg tggatcatta ttgcagcagt gttcgtctat aagatctcgg
     6481 tgaaaaccgg gcagtttgac atcattcgct cgtctattct ttcgataacc cctgaccagc
     6541 gtctgcaaat gctgatcgtc ggtttctgtt tcggcgcttt ccttgaagga gccgcaggct
     6601 ttggcgcacc ggtagcaatt accgccgcat tgctggtcgg cctgggtttt aaaccgctgt
     6661 acgccgccgg actgtgcctg attgttaaca ccgcgccagt ggcatttggc gcgatgggca
     6721 ttccaatcct ggttgccgga caagtaacag gcatcgacag ctttgagatt ggccagatgg
     6781 tggggcgtca gctaccgttt atgaccatta tcgtgctgtt ctggatcatg gcgattatgg
     6841 acggctggcg cggtatcaaa gagacgtggc ctgcggtcgt ggttgcaggc ggctcgtttg
     6901 ccatcgctca gtaccttagc tctaacttca ttgggccgga gctgccggac attatctctt
     6961 cgctggtatc actgctctgc ctgacgctgt tcctcaaacg ctggcaacca gttcgcgtat
     7021 tccgcttcgg tgacttgggg gcgtcacagg ttgatatgac gctggcccac accggttaca
     7081 ccgcgggtca ggtgttacgt gcctggacac cgttcctgtt cctgaccgct accgtaacac
     7141 tgtggagcat cccgccgttt aaagccctgt tcgcatcggg tggtgcgctg tatgagtggg
     7201 tgatcaacat tccggtgccg tacctcaata aactggttgc ccgtatgcca ccagtggtca
     7261 gcgaggctac agcctatgcc gccgtgttta agtttgactg gttctctgcc accggcaccg
     7321 ccattctgtt tgctgcactg ctctcgattg tctggctgaa gatgaaaccg tctgacgcta
     7381 tcagcacctt cggcagcacg ctgaaagaac tggctctacc catctactcc atcggcatgg
     7441 tgctggcgtt cgcctttatc tcgaactatt ccggactgtc atcaacactg gcactggcac
     7501 tggcgcacac cggtcatgca ttcaccttct tctcgccgtt cctcggctgg ctgggggtat
     7561 tcctgaccgg gtcggatacc tcatctaacg ccctgttcgc cgcgctgcaa gccaccgcag
     7621 cacaacaaat tggcgtctct gatctgttgc tggttgccgc caataccacc ggtggcgtca
     7681 ccggtaagat gatctccccg caatctatcg ctatcgcctg tgcggcggta ggcctggtgg
     7741 gcaaagagtc tgatttgttc cgctttactg tcaaacacag cctgatcttc acctgtatgg
     7801 tgggcgtgat caccacgctt caggcttatg tcttaacgtg gatgattcct taatgattgt
     7861 tttacccaga cgcctgtcag acgaggttgc cgatcgtgtg cgggcgctga ttgatgaaaa
     7921 aaacctggaa gcgggcatga agttgcccgc tgagcgccaa ctggcgatgc aactcggcgt
     7981 atcacgtaat tcactacgcg aggcgctggc aaaactggtg agcgaaggcg tgctgctcag
     8041 tcgacgcggc ggcgggacgt ttattcgctg gcgtcatgac acatggtcgg agcaaaacat
     8101 cgtccagccg ctaaaaacgc tgatggccga tgatccggat tacagtttcg atattctgga
     8161 agcccgctac gccattgaag ccagcaccgc atggcatgcg gcaatgcgcg ccacacctgg
     8221 cgacaaagaa aagattcagc tttgctttga agcaacgcta agtgaagacc cggatatcgc
     8281 ctcacaagcg gacgttcgtt ttcatctggc gattgccgaa gcctcacata acatcgtgct
     8341 gctgcaaacc atgcgcggtt tcttcgatgt cctgcaatcc tcagtgaagc atagccgtca
     8401 gcggatgtat ctggtgccac cggttttttc acaactgacc gaacaacatc aggctgtcat
     8461 tgacgccatt tttgctggtg atgccgacgg ggcgcgtaaa gcaatgatgg cgcaccttag
     8521 ttttgttcac accaccatga aacgattcga tgaagatcag gctcgccacg cacggattac
     8581 ccgcctgccc ggtgagcata atgagcattc gagggagaaa aacgcatgat tatttccgca
     8641 gccagcgatt atcgcgccgc agcgcaacgc attctgccgc cgttcctgtt ccactatatg
     8701 gatggtggtg catattctga atacacgctg cgccgcaacg tggaagattt gtcagaagtg
     8761 gcactgcgcc agcgtattct gaaaaacatg tccgacttaa gcctggaaac gacgctgttt
     8821 aatgagaaat tgtcgatgcc ggtagcactg gcaccggtgg gtttgtgtgg catgtatgcg
     8881 cgtcgtggcg aagttcaggc agccaaagcg gcggacgcgc atggcattcc gtttactctc
     8941 tcgacggttt ccgtttgccc gattgaagaa gtcgcgccag ccatcaagcg cccaatgtgg
     9001 ttccagcttt atgtactgcg cgatcgcggc tttatgcgta acgcgctgga gcgagcaaaa
     9061 gcagcgggtt gttcgacgct ggttttcacc gtggatatgc cgacaccggg cgcacgctac
     9121 cgtgatgcgc attcaggtat gagcggccca aatgcggcaa tgcgccgcta cttgcaggcg
     9181 gtgacgcatc cgcaatgggc gtgggatgtg ggcctgaacg gtcgtccaca tgatttaggt
     9241 aatatctcag cttatctcgg caaaccgacc ggactggaag attacatcgg ctggctgggg
     9301 aataacttcg atccgtccat ctcatggaaa gaccttgagt ggatccgcga tttctgggat
     9361 ggcccgatgg tgatcaaagg gatcctcgat ccggaagatg cgcgcgatgc agtacgtttt
     9421 ggtgctgatg gaattgtggt ttctaaccac ggtggccgcc agctggacgg tgtactctct
     9481 tccgctcgtg cgttgcccgc tattgcagat gcggtgaaag gtgatatcgc cattctggcg
     9541 gatagcggaa ttcgtaacgg gcttgatgtc gtgcgtatga ttgcgctcgg tgccgacacc
     9601 gtactgctgg gtcgtgcttt cctgtatgcg ctggcaacag cgggccaggc gggtgtagct
     9661 aacctgctaa atctgatcga aaaagagatg aaagtggcga tgacgctgac tggcgcgaaa
     9721 tcgatcagtg aaattacgca agattcgctg gtgcaggggc tgggtaaaga gttgcctacg
     9781 gcactggctc cgatggcgaa agggaatgcg gcatagtcgt ttgcgccccc tcaccctaac
     9841 cctctccctc agggagaggg gaccgttcgg cgctgtatgt actccctcac tctgaaacga
     9901 caccgcactc tttttttctc cctcgcccct ccggggaggg ccggggtgag gggaaaaggc
     9961 cgcacggttc accaattttc tgctatcctg cccccgcact aagggggcag tatgctaaac
    10021 atcgtacttt acgaaccaga aattccgcca aatactggca acatcatccg tctttgcgct
    10081 aataccggct ttcgtctaca tatcatcgaa ccgatgggat ttgcctggga cgacaagcgc
    10141 ctgcgccgcg cggggctgga ctatcacgag tttaccgccg ttacgcgtca tcatgactat
    10201 cgcgcgttcc tcgaagcaga aaatccccag cgcctgtttg ccctcaccac gaaaggtacg
    10261 cctgctcaca gcgccgtaag ctatcaggat ggcgattatc tgatgttcgg cccggaaaca
    10321 cgcggcctgc cagcgagcat tcttgatgcc ctgcccgctg aacaaaaaat tcgcattccg
    10381 atggtgccgg acagccgcag catgaatctg tccaatgcgg tgtcggtagt ggtgtatgaa
    10441 gcctggcggc agctgggata tccgggagcg ttgttgagag attagttact gtatgccgga
    10501 tagatgatta catcgcatcc ggcacgatca caggacatta gatcccatcc ccatactcaa
    10561 atgtatggtt aataccgttg aaatgctggt ccatatccat tgatggctta tcgctgtctg
    10621 gtttaccgac aatacgagcc ggaacgccag cggcggttgt atgcggcggc accggttgca
    10681 gcaccacgga acctgcgcca atcttcgcgc cgcgaccaac ttcaatattg ccgaggattt
    10741 tcgcgcccgc gccaatcatc acaccttcac gaattttcgg gtgacggtca ccaccagatt
    10801 tacccgtacc gccaagcgtc acagattgca gaatcgatac gtcgttttca attaccgccg
    10861 tttcacccac aacgatgccc gtggcgtggt cgagcataat gccacggcca atttttgctg
    10921 ccgggtgaat atcgacctgg aacgtcacag aaacctggtt ttgcagaaag attgccaatg
    10981 cgcgacgccc ctgattccac aaccagtgac cgatgcgata ggcctgcaag gcatgaaaac
    11041 ccttcaggta taacaacggg gttgagtatt tatcgactgc cgggtcgcgg gtacgcaccg
    11101 cctgaatatc acaggccgca gaggcgatca tttccgggtc agcggcgtag gcttcttcaa
    11161 ccacttcacg gatagcaata gcaggcataa ttggcgatga cagcttgttc gccagcatgt
    11221 agctcagtgc actgccaagg ttttcgtgct tgagtagcgt cgcgtggtaa aaactggcca
    11281 gcattggctc acagtccgcc agcgttctgg cttcggcttt aatattgttc cagacaattt
    11341 ccagttcttc acacgacatt gcttactcca cacgatgaga taatgaccgg cccgttctgc
    11401 gcgggccggg tcatagcggt aacaaaggtt ccctggggtt agtgactgct gcgctcgtcc
    11461 ttgcgtgcac gacctaataa tgtcaatgct gcctcgcgcg cgttttttcc gcaatataat
    11521 acttgataaa tttcctcggt tattggcatt tcaacgccga agcgatgcgc cagttcgcgg
    11581 acttctttcg tattgcggta gccttccacc acctgaccaa tcttctcctg cgcgctttgt
    11641 acatccatgc cctgaccgag catcatacca aaacggcggt tacgcgactg gttgtcggta
    11701 caggtaagca ccagatcgcc cagccccgcc atgcccataa aggtggcagg atcggcaccc
    11761 agcgccgcac caagacgcga catttcagcc agcccacggg tgatcagcgc cgtacgcgca
    11821 ttcgcaccaa aaccgatacc gtcagacatc cccgcgccaa tggcaatgac gtttttcacc
    11881 gcgccgccaa gctgcacgcc aatgaaatcc gggttgctgt aaacgcggaa acttttgccg
    11941 cagtgcagca gctgctggag atcatcggca aaggtctgat cggtcgaggc cagcgaaata
    12001 gctgtcggta aacctgccgc cagttctttc gcaaacgttg ggccagagat aaccgccagc
    12061 ggaatttgat cgcctaaggc ctcacgcgcc acgtcctgta acagacgtcc ggtttccgct
    12121 tccagccctt tggtcgccca caccagacgc gcatcaggac gcatcagcgg tttaatctgg
    12181 cgcagcactt caccaaagac atggctgggt acgacgacga gaatattacg actggctgcc
    12241 agcgcagtgg cgagatcgct ttcaagatgg agcgtatcgg gaaaaggcac atcggggaga
    12301 aacgcggcgt tacagcggtc gcgttcaagc gttgcgatat gttcagggtc atggccccag
    12361 aggacaacct cgtggccatt tcttgccagg gtaatggcaa gagcggtgcc gtacgagccg
    12421 gcaccgatca cagtcattga agcattacgt tggttcatca ggcatcctga tgttcttcag
    12481 taccttcgcc agcctgctgc tgcaaatagt tcatgaacag cgcatcgaag ttaaccggcg
    12541 caaggttcag ttgcgggaat gtaccgcggg ataccatgct ggtgatgcac tcacgagcat
    12601 acgggaacag aatgttcggg cagtatgcgc ccaggcaatg tgccatctgg gtgccttcga
    12661 tacccgcgat ggagaaaata ccgccctgct gaacttcaca caggaacgcg gtttcttcgc
    12721 ccaaggaggc cgttacggta acacgcagta ccacttcgta tacgtcatct gccagttggg
    12781 aagatgccgt atccagatca agtttaactt ctggttgcca atctttctgg aaaacgtgcg
    12841 gcgcgttcgg cgcttcgaaa gagatatcct tggtataaat acgttggatc tggaaagtca
    12901 tttcagtgtt gttttgttct gacatgtgta gaaaaccctt aagtgttgtc cttaaatact
    12961 gcgcaatgta ataccaacgt tcacacgtta tttcagcagg ggatccagtc caccacgtgc
    13021 atccaatgca tacaagtcat cacagccgcc aatgtgctgt gcgtcaataa aaatctgggg
    13081 aaccgtggtg caaccgctgc gtttgatcat ctcttcacgc ttggcggcat tgccatcgat
    13141 cggcaactcc tggaaactca cgcccttgct gctcagcagt gcttttgcac gatggcaata
    13201 cgggcaggtt tctttggtat agatttcaac attggccata atttagctcc tgttttttta
    13261 ccctgaaatt tcatgttgca gggcggctgc aaaatggagt gtccccagaa gcttataaca
    13321 ataagtcgct gggggcctaa ttgcagctaa cggcctgcat catgaaagac gacaggtaaa
    13381 ttatttgccg cgcaccagag gcaagttttc gccagcccag ccagcaacgc cctctttcag
    13441 cacgaatact tgcgcaaaac cagctttcgt cagcgcgttt gcaggctcct ggcactgcat
    13501 gccagaaccg tctaccacga taaccgattt gtctttgtgc ttctcaagct caccaacatt
    13561 gttggctttg atttcgctcg gcaacagatt gatagaacct gcgatatggc ctttacggaa
    13621 gtcatcacgc tgacgtaaat ccacaaccac agcgtcttct ttgttgatca gacgcgtagc
    13681 ttcaccacga gtaatcacct tcactttcga ggtcaggctc ttaaacgtag tcacaagaac
    13741 cgccaccagt aacgcgatcc aggcgatact cagtatggga tgacggccaa caaattgcat
    13801 aatttcttgc atggggggta acaactcccg acgtagtgat taaaaaaacc aggaaaggag
    13861 tatacctgcg cggtgcggca aatacagcca gcgcgttaac tggaatgcaa ttttgcgggg
    13921 cacgacgaaa aaaaagcgca aatctggcat cggcttgccg cgagcggtcg tatattttga
    13981 tcttcagagg ctattttatc gattcagctg tagtaaaatt acgcaaattt tgactcttga
    14041 gtatgaggtt gtcgcaatgt cggtttctaa aaaacctatg gtactggtga ttctggatgg
    14101 ctatggctat cgtgaagaac agcaggataa cgccattttt agtgctaaaa ccccggtaat
    14161 ggatgcactg tgggccaatc gtccgcatac cctgatcgac gcttccggtc tggaagtcgg
    14221 tctgcctgac cgtcagatgg gtaactccga agtaggccac gttaacctgg gtgccggccg
    14281 catcgtgtat caggacctga ctcgtctgga cgttgaaatc aaagatcgcg ctttctttgc
    14341 taatccagtg ctggctggcg cggtacataa agcgaaaaac gcaggtaaag cagtacacat
    14401 tatgggtctg ctctccgcag gcggcgtaca cagccacgaa gatcacatca tggcgatggt
    14461 agaactggca gctgaacgcg gcgcagaaaa aatctacctg cacgcattcc ttgacggtcg
    14521 cgacaccccg ccgcgcagtg ctgaatcctc gctgaaaaaa tttgaagaga aatttgctgc
    14581 gctgggtaaa ggccgcgtag cgtccatcat tggtcgttac tacgcgatgg accgcgataa
    14641 ccgttgggat cgcgttgaaa aagcctatga cctgctgact ctggcgcagg gtgagttcca
    14701 ggccgatact gccgttgctg gtttgcaggc agcttatgct cgcgacgaaa acgacgagtt
    14761 cgtgaaagcg accgttattc gtgcagaagg tcagccagat gcggcaatgg aagacggcga
    14821 tgcgctgatt ttcatgaact tccgtgctga ccgcgcgcgt gaaatcactc gtgcattcgt
    14881 gaacgctgat ttcgatggct tcgcgcgtaa gaaagtggtt aacgtcgatt tcgtgatgct
    14941 gaccgaatac gccgctgaca tcaaaactgc ggttgcttac ccacccgctt ccctggttaa
    15001 caccttcggc gagtggatgg cgaaaaacga caaaactcag ttgcgtattt ccgaaaccga
    15061 aaaatatgcc cacgttactt tcttcttcaa cggtggcgta gaagagtcgt tcaaaggcga
    15121 agatcgcatt ctgatcaact caccgaaagt ggctacctac gatctgcaac cggaaatgag
    15181 ctccgcagag ctgaccgaaa aactggttgc ggccatcaag agcggcaaat acgacaccat
    15241 tatctgtaac tatccgaacg gcgacatggt aggtcacacc ggggtgatgg aagcggcggt
    15301 taaagcggtt gaagcactgg atcactgcgt ggaagaagtc gcgaaagcgg ttgagtccgt
    15361 gggtggacaa ctgctgatca ccgctgacca cggtaacgct gaacagatgc gcgatccggc
    15421 aacgggtcag gcacacacgg cacacaccaa cctgccagtt ccgctgattt acgttggtga
    15481 taagaacgtg aaagcggttg caggcggcaa actttctgac atcgcgccga ccatgttgtc
    15541 gctgatgggt atggaaatcc cgcaagagat gactggtaag ccgctgttca tcgtggaata
    15601 atccctcccc atgaggggaa aggcgattaa taccatgaca cgggccgtga aaccgcgcag
    15661 gtttgcaatc aggcccatca tctacgccag cgttcttagc gctggcgtat tgttgtgcgc
    15721 cttttccgcc cacgcggatg agcgtgacca actcaaatct attcaggccg atatcgccgc
    15781 aaaagagcgc gcggtacgcc aaaagcaaca acaacgcgca agcctgctcg cacaattgaa
    15841 aaagcaggaa gaagcgatct ctgaagccac ccgtaagctg cgcgaaacgc aaaacacgct
    15901 caatcaactc aataaacaga ttgatgagat gaacgcgtcg attgccaaac tggagcagca
    15961 aaaagccgcc caggagcgca gcctcgccgc acaactggat gccgcattcc gtcagggcga
    16021 gcataccggt attcagctaa ttctcagcgg tgaagaaagc cagcgtggac agcgtttaca
    16081 ggcttatttc ggctatctca accaggcgcg acaagaaacc attgcccagt tgaagcaaac
    16141 gcgtgaagaa gtcaccatgc agcatgctga actggaagag aaacaaagcg agcaacaaac
    16201 gctgttatat gaacagcgcg cccaacaggc gaaactgact caggctctga acgagcgtaa
    16261 aaagacgctg gcggggctgg aatcttccat ccagcaaggt cagcagcagc taagtgaact
    16321 gcgtgccaac gaatcccgtc tgcgtaacag cattgcccgt gctgaagccg cggcgaaagc
    16381 gcgtgctgaa cgagaagcgc gtgaggccca ggcggttcgc gagcgccaga aagaagcgac
    16441 gcgcaaaggc accacctaca aaccgaccga aagcgaaaaa tcgctgatgt cccgaactgg
    16501 tggcctgggt gcgccgcgtg gtcaggcatt ctggccggtt cgcgggccga cgctgcatcg
    16561 ctatggcgag cagctacagg gtgaattacg ctggaaaggg atggttatcg gtgcctctga
    16621 aggtactgaa gttaaagcaa ttgccgacgg tcgagtgatt ctggctgact ggctgcaagg
    16681 ttacggtctg gtggtggtgg ttgagcacgg taaaggcgac atgagtcttt acggctataa
    16741 tcagagcgca ctggtgagcg ttggttcgca ggttcgcgcg ggccagccaa ttgcactggt
    16801 gggcagcagt ggcggtcagg gtcggccttc actctatttc gaaattcgcc gccagggtca
    16861 ggcggtcaat ccacagccgt ggttgggaag ataagttttg tttccatttc gtcgtaacgt
    16921 tcttgcattt gccgctctgt tggcgctctc ctcccccgta cttgctggca aacttgccat
    16981 cgtcattgat gattttgggt atcgcccgca caacgaaaac caggtgctgg cgatgccttc
    17041 cgctatctcc gtcgctgtat tacccgattc accgcacgcc agagagatgg cgaccaaagc
    17101 gcataacagc ggtcacgaag tgttgattca tctcccgatg gcaccgttga gtaaacagcc
    17161 gctggagaaa aatacgctac gcccggagat gagcagcgac gaaattgagc gcattattcg
    17221 tagtgcggtc aataacgtgc cctatgccgt ggggatcaac aaccacatgg gcagcaagat
    17281 gacctctaac ctgtttggta tgcagaaagt gatgcaggcg ctggagcgtt acaatcttta
    17341 cttcctcgac agcgtaacca tcggtaatac ccaggcgatg cgcgccgcgc aaggtactgg
    17401 cgtgaaggtg atcaaacgga aggtgttcct cgacgattcg cagaacgaag cggacatccg
    17461 tgtgcaattt aatcgcgcaa ttgatctggc gcgtcgcaac ggttcaacca ttgccattgg
    17521 gcatcctcac ccttcaacgg tacgcgtgct acaacagatg gtttataacc tgccgccaga
    17581 cattacgctg gtgaaagcca gcagcttgct gaatgaaccg caggttgata cttctacacc
    17641 gccgaaaaac gctgtgcctg atgcaccgcg taatccgttc cgtggcgtga agctgtgcaa
    17701 accgaagaaa ccgatagaac ctgtttatgc taatcgcttc tttgaagtat taagcgaaag
    17761 catcagccag agcacgctga tcgtttactt ccagcatcag tggcaaggct ggggcaaaca
    17821 gcccgaagcg gcgaagttta acgctagcgc aaattaaagg cgctacgcgt aatgcgatgg
    17881 tgcgaccgcg ttttgtcgcg ccattgccat aagcggaaag accacagtaa cgcctggtaa
    17941 ccgaccttca cgctgcgcac attggtaatc aggcgcttat acatacccgg aagtaaatat
    18001 ctcggcaatc atccgttggc gggtaagaat atccggctct ttgcg
//
LOCUS       DAJTSA010000083        17644 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000083 DAJTSA010000000
VERSION     DAJTSA010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.083"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..671)
                     /gene="chbR"
                     /locus_tag="OO369_002958"
                     /pseudo
     CDS             complement(1..671)
                     /gene="chbR"
                     /locus_tag="OO369_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(679..1029)
                     /gene="chbA"
                     /locus_tag="OO369_002959"
     CDS             complement(679..1029)
                     /gene="chbA"
                     /locus_tag="OO369_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR5983158.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="OO369_002960"
                     /pseudo
     CDS             complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="OO369_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="OO369_002961"
     CDS             complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="OO369_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR5983159.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="OO369_002962"
     CDS             complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="OO369_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR5983160.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3680..4507
                     /gene="nadE"
                     /locus_tag="OO369_002963"
     CDS             3680..4507
                     /gene="nadE"
                     /locus_tag="OO369_002963"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR5983161.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4737..5624
                     /gene="cho"
                     /locus_tag="OO369_002964"
     CDS             4737..5624
                     /gene="cho"
                     /locus_tag="OO369_002964"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR5983162.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5584..6159)
                     /gene="ves"
                     /locus_tag="OO369_002965"
     CDS             complement(5584..6159)
                     /gene="ves"
                     /locus_tag="OO369_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR5983163.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6362..6847)
                     /gene="spy"
                     /locus_tag="OO369_002966"
     CDS             complement(6362..6847)
                     /gene="spy"
                     /locus_tag="OO369_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR5983164.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANLKKRLTERPAAKGKMPATA
                     E"
     gene            complement(7177..8145)
                     /gene="astE"
                     /locus_tag="OO369_002967"
     CDS             complement(7177..8145)
                     /gene="astE"
                     /locus_tag="OO369_002967"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR5983165.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(8138..9471)
                     /gene="astB"
                     /locus_tag="OO369_002968"
                     /pseudo
     CDS             complement(8138..9471)
                     /gene="astB"
                     /locus_tag="OO369_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9468..10946)
                     /gene="astD"
                     /locus_tag="OO369_002969"
     CDS             complement(9468..10946)
                     /gene="astD"
                     /locus_tag="OO369_002969"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCR5983166.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10943..11977)
                     /gene="astA"
                     /locus_tag="OO369_002970"
     CDS             complement(10943..11977)
                     /gene="astA"
                     /locus_tag="OO369_002970"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR5983167.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11974..13194)
                     /gene="astC"
                     /locus_tag="OO369_002971"
                     /pseudo
     CDS             complement(11974..13194)
                     /gene="astC"
                     /locus_tag="OO369_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13640..14446
                     /gene="xthA"
                     /locus_tag="OO369_002972"
     CDS             13640..14446
                     /gene="xthA"
                     /locus_tag="OO369_002972"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR5983168.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14613..15323
                     /locus_tag="OO369_002973"
     CDS             14613..15323
                     /locus_tag="OO369_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5983169.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            15328..16005
                     /gene="ydjY"
                     /locus_tag="OO369_002974"
     CDS             15328..16005
                     /gene="ydjY"
                     /locus_tag="OO369_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR5983170.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            16020..16727
                     /locus_tag="OO369_002975"
     CDS             16020..16727
                     /locus_tag="OO369_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5983171.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16727..17275
                     /locus_tag="OO369_002976"
     CDS             16727..17275
                     /locus_tag="OO369_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR5983172.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            17285..17611
                     /locus_tag="OO369_002977"
                     /pseudo
     CDS             17285..17611
                     /locus_tag="OO369_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         4364 a         4685 c         4308 g         4287 t
ORIGIN      
        1 tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat
       61 tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct
      121 ttatcatgca tcttttctac cgtacttttc agccactacg gtacatcatc aatcacctgt
      181 tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct
      241 tccagccctg tttcgcggaa atttaatgaa gaaatcactg tttccacata ggtgagaaaa
      301 gcgttattgg tccggtatac ctgcgaagca acaaagcaat aaggcaacaa cggcaggtaa
      361 tgctgctcaa aaaagcgttt actgatccca acgttcaata tgcgcgtggc accaaactca
      421 taaaaacttt gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac
      481 acgcgcttac cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa
      541 tagtcgtgct gatgcagtcc gctgatactc tcagttttgt tatagataaa cacatggaag
      601 tttttgccat taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact
      661 ggctgcatca tcgactcctt atgccttcag tttttcatga agctcaatta attcagtaat
      721 cagttcacgc gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact
      781 cactttcatc ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc
      841 ttcattcaat gccatccgtg actgatccat catggctttc gctgcggcaa aatcgccctg
      901 cttcgcctgt ttcaatgcgg catacgccag gctacgcgct tgtccagagt tgatgataag
      961 ccccatcacc acttcttcca gctcttcagc ttctgtttgc gtatcaggaa tattatcgag
     1021 atccatcata catcgttcct ctctcttctt accggcaaga ttacccgtac cggcatcgat
     1081 taaaatttca ggggttagcg atatcttctt cgctctcttc tttatcaatc gcattttgtg
     1141 ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatgcca aggttgaaga
     1201 gtgcgaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc agaccggttg
     1261 gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag tacgctgcga
     1321 gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc accgggttca
     1381 tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg gacggcagcg
     1441 ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata gccaggatca
     1501 ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc ggcttggccc
     1561 agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat tgctgatagg
     1621 tcgcgatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca
     1681 gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag gcccagccca
     1741 ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc tgatggaagt
     1801 tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga ataataaagc
     1861 cgggaattaa tgccgagaaa gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa
     1921 tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg ccaataataa
     1981 tcccggagat gatattcgcc ccacctaacc agtttgcgcc aaccgcatag gcctcaccga
     2041 cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc gccagcgcat
     2101 cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt aaagacatta
     2161 ttcctaatgt tccgttatat acgttgccgc caataccttt cagaccatta agtgtttcaa
     2221 tggttgaggc gtcgaggcga atacctaagg aataaaaaac gacccctccc caaagcttag
     2281 aaaaacgttg ttaattaata caaacatggc ccccgcaagg gttaacggca ttaagcgaat
     2341 aaagccattt ttgattgcat taacgtgtgg ctgctttcct attttaactg caaaagggag
     2401 gagtaccttt tcaagcgatg caataacatt actcatagaa aaataccctt aaaaaccgca
     2461 atttaaatat tacggtattg atttatgaaa taactctttg acgggaaaat ttaaaataaa
     2521 ttaatttgct gcggcttttt taatcgctgc aaccgcagcc ttaagcacgc ctaaaccatc
     2581 gactttgcca taaagcagcg agtcaattac ttcaaccggt ttgttgggta acaaacgctg
     2641 gatttcgggc aacatataag caatctgcgg ccctaataac acgacatcgg cattctgacc
     2701 tttttcacca gccagtgttt ccggaaatgc ttcaataatg accggaactt catatttttc
     2761 tgcctgtgcg cgcatttttg ataccagtaa agagatagac atgcccgcag aacaaaacag
     2821 ataaatgtgt ttcttttcca taaaactgcc ctcatcgacg attatctgtc agccagacac
     2881 tccgcaagcc ttgacctgct tccatgctct gggtaacttg cgaaaccaac atgacttttt
     2941 ttgtggctga aaggagtata cggtatagac ctgagcaata gtatttcttt gaccttataa
     3001 attgtttctc cttgacctgt ggttatgacc ctcttcacat ttcgggcaaa taattcgcga
     3061 agataattaa gaactcaggc caaaaaaaac cggcgcaatg gccggtttcc gttgttactc
     3121 aagctttcag acgaattgat tacttcgcag cctgtggatc agtgtcgtat tcagcacagg
     3181 tctgataacc ggagttgatg acatgtccgg tatcatctaa cgcgacaaag taggtttctg
     3241 ctttaccatc acgttgaccc aggatgtagg tctggcaagt accgcgagca tggatcatgc
     3301 tcacttcaga cgaaggttta cccgcaattt gtgcaacctg cgcccggctc atgccttttt
     3361 tgacgtcttt caccacaggc tgtacaaact ggtctttggt acgatcataa gctgtacaac
     3421 ccgccagcat ggttaatacc gccgctgcac tcagaattcc tgccatattc ttgttcatat
     3481 tccgtcctct tgtttatcag cgtgttagat aagcctggaa tacattgggc gctttttcaa
     3541 gcccgtgaac gaaacggctc cgctttcaga ggattcctgt atgacgtttt aaccaccatt
     3601 cagcccgctg tcgcttgtcg tttcagtagc aacgggttag ctttaaggaa gttttgtctt
     3661 ttctgtctgg aggggttcaa tgacattgca acaacaaata ataaaggcgc tgggcgcaaa
     3721 accgcagatt aatgctgaag aggaaattcg tcgtagtgtc gattttctga aaagctacct
     3781 gcaaacttat ccgttcatta aatcactggt gctcgggatc agcggcggtc aggactccac
     3841 gcttgccgga aagctgtgcc agatggcgat taatgagctg cgccaggaaa ctcgcaacga
     3901 atcactgcaa tttattgccg tacgcctgcc ctatggtgtt caggctgacg aacaagattg
     3961 ccaggatgcc attgccttta ttcaaccgga tcgcgtatta accgttaata tcaagggcgc
     4021 ggtattggct agcgagcagg cattgcggga agcaggcatt gaactgagcg attttgtccg
     4081 tggcaatgaa aaagcgcgtg agcggatgaa agcacaatat agcattgcgg gtatgaccag
     4141 cggtgtcgtg gtgggcaccg atcatgcagc agaagccatt accggattct tcactaaata
     4201 tggtgacggc ggtacggata ttaatccgct gtatcgtctc aacaaacgcc agggtaaaca
     4261 gttactgacg gcattaggtt gcccggaaca cctttataag aaagccccaa ctgccgatct
     4321 tgaagatgat cgcccttctc tgccagatga agtggcactc ggcgtgacct atgacaatat
     4381 cgacgactat ctggaaggga aaaacctacc tgaacaggtc gccagaacaa tagagaactg
     4441 gtatctgaaa accgaacata aacgccgtcc gccaattaac gttttcgatg atttctggaa
     4501 aaagtaataa tttgcaccta ttggattgtg cgaaaaagag taatttgttc acgccggatg
     4561 cggcgtgaac gccttattct acctacaaaa ctatgcaaat tcaatacatt gcaggagtcg
     4621 aataggcctg acaggcgtag cacgtcagac ggtgtaacct ttgtcatcaa cccgcctctt
     4681 ttttaatcgc ctcccgcctg ttacactgga tagataacca gcattcggag tcaacagtgg
     4741 tacggcgttt aacttctccg cggctcgaat ttgaagctgc ggcaatttat gaatatcccg
     4801 aacatttaca ttcattcctt aatgacttac ccacccgacc aggggtgtat ctgtttcatg
     4861 gcgaaagtga caccatgccg ctctatatcg gcaaaagcgt taatatccgc agtcgggtgc
     4921 tttctcattt acgcaccccg gatgaagccg ccatgctacg gcaatcccgg cgaattagct
     4981 ggatctgcac cgcaggcgaa atcggcgctc tgctccttga agcgcgatta atcaaagaac
     5041 aacagccgct gtttaataaa cggttgcgcc gcaatcgcca gctctgtgcc ctgcaattaa
     5101 atgaaaagcg cgtcgatgtg gtgtatgcca aagaggtgga tttttcacga gcccccaacc
     5161 tgtttggcct gtttgccaat aggcgcgcag ctttgcaagc attgcagagc atcgctgatg
     5221 aacaaaaact ttgttatggc ctgttgggac tggaaccgtt aagtcgcggt cgtgcatgtt
     5281 ttcgttcagc gctaaaacgt tgcgccggag catgctgcgg taaagagagc catgaggaac
     5341 atgcgctacg cttgcgccag tctctggagc gtttgcgagt ggtgtgttgg ccttggcaag
     5401 gtgcggtggc actgaaagaa cagcacccgg aaatgactca atatcatatt attcaaaact
     5461 ggctgtggct gggggcggtt aattcgctgg aagaagcgac aacgttaatt cggacacccg
     5521 ccgggtttga tcacgacggt tataaaattc tttgtaagcc gctgctttcc ggtaactatg
     5581 aaattactga acttgatccg gcgaatgacc agcgagccag ttgatttcac tgaacaacag
     5641 tttaccttgc ggttgtaaaa gccgcagggt gtgcctgcca tcgaaccagc acacgccctg
     5701 gtcggtagtg agcagtttgt cgcccagttg ccacgccccg ttaatcacaa acaccacccc
     5761 gccgcgtgag ccaaaggtag taaacgtacg ttcggcaatt ctgactttcg ccttacagac
     5821 atccaggcgc gtcataatat tgaaatccat cgacatctgc cctgccgtca gtttcgcttt
     5881 taccacctgg tccgccgtaa aggcaaaagg ctgtaacggt tttaaggtat ggttaaagcg
     5941 gtctgcgctt tcaaggagca tctcgccgcc ttccagcaac gtcactatcc tttccatgcc
     6001 gggaaacaaa gaaaactccc cattcgccgc gatggatgca atgctggcac gccagtaaaa
     6061 atcacgtttt gccggtggga aagtgcaaat ttcgcgcgtt tcaccggcag cgtttcgcca
     6121 cagattcacc gacattttac gcatatcaaa gtattccata ctcgctcccg gcccgttctt
     6181 ttcattgtta tcgttcaggt aaccgtggat ggttatcggc aactcgggtg atttacgtta
     6241 gtggtgatca ggcttttttt acaactgttt gagaagagaa aagaaaaccg ccgatcctgt
     6301 ccaccgcatt actgcaaggt agtggacaag accggcggtc ttaagttttt tggctgaaag
     6361 attattcagc agttgcaggc attttacctt ttgccgctgg acgttctgtc agacgcttct
     6421 ttaaattagc attaaattgc tttttctgtt ccggcgtcag gatgttgtaa attttgttct
     6481 gggtttccat gtgcgccagc atgttagctt tgcgctgttc ttccattttt gcgatctgcg
     6541 cttcagcttt tgctttatcg aaggtatcgc tggcaatgat gtcatgcatt gcgcggcgtt
     6601 cttccagcgg cggacgtttc atctggtcac gctggccttt catgatttcg tggatctgct
     6661 gtttctgcgc gtcggtcagg ttcaggtctt tgaacatcat gtcctgatgc ggaccgaact
     6721 tgcctttgtg gtgcatcatc ggcttcgcgt cagccggtgc tgcggtagtg gtgtctgcgg
     6781 catgggccag gttagccgcg ccaagagcca gggtagaggc aacaaacagt gcagttaatt
     6841 tacgcatatt ctatatcctt cctttcagtt atttattacg gctttcttaa gtagcgtgcc
     6901 gtgttgacga gattaacttt actgacttta gcgtcaatta atcagagcaa cggtaaaaca
     6961 atgaaagtgt aaaaaacact tttgcgccaa ttatggagaa aaaaagaaaa tttgatggag
     7021 agtgatgaga gaatattaca acacgatgat tttgcagaga ttatgaagaa ctataccgga
     7081 tgactggtga taaataaagc aaataaccag gattaatctg tattaattta taagaaagca
     7141 acttaatacc cgcagaatga tttctgcggg taagtattag cttatttttt cgagcattaa
     7201 tcccgcgcgt aatcccaacg ctaccaacgg attagggaat aacacatact ctacatcatg
     7261 ggttacggta aaacgttcct ctccgtcctg cgccagcaat gttcctttct caaacggcat
     7321 aaaattcagc gtgtcacttg ccatatgcat ttcgaaggac ggcgagtggc gagtaatttg
     7381 cgaaaccacc cgataacgga gcggcggtgt tctcacgata ccgacactct caccagatag
     7441 cagcgcagca attgcgctgg cagttactgc aaactggcga agatcgtttt gcccaaaggg
     7501 caacgctttg ccaagttcca acgtacaggc cagcgcgcca aaatgtctga cgctgaaatg
     7561 ggtaaacgta ccaccaggct cctgatggaa caccagcgcc tccagccccg ccgcacccag
     7621 ccacgtcaga aatttctcgt cccagggaat gtcgcgttgc ggtaatacac cgaactgcgg
     7681 atgcaaggag ccacgaattg cggtatgtag atcaaggtgc caacgcacag attctttgcc
     7741 ctggtcataa aaatcttcca ggcactgttc cagttcgcgc gcccgacagg tttctccgct
     7801 ttcagcaaat agctgccaac gaccgccaaa cattcggttc atatcgctat ggcaataacg
     7861 tttcccttgc ttcagcgcag gaggattccc gaggatcacc agcaaccgcc aacgtaacgg
     7921 gatctcgccg tgagatatcg cgccaagcaa cgcgtccagc atttccacag gtgccgtctc
     7981 attaccgtgg atccccgcag aaatcaccag cgcgccttgc ggtggcgtta atggcgtcag
     8041 ttccagcaca ccatcgccca gccagcgcca gcgaacgccg ttgatttctc gctcggtgat
     8101 aaccggtttt ttacccgtta aggtcagagc aagaaaatta tccattgccc cctccctcgc
     8161 gctggaacgg ataaaccgaa ccgagattca gtaattggct caatacatcc agtgcttccc
     8221 gcccttcgcg cagcaattgc gggtcggcca gatcggcagc agtaagacga tcgcggtagt
     8281 aacgatccac ccagtcattg agcgcattaa acaaggtatc gttcatcatc accgccggat
     8341 tcaccgcccg gcgttcttct tctgtcaata ccacccgcaa ccgcaggcac gccgggccgc
     8401 cgccattcgc catgctttca cgtaagtcaa agacttttag ttcgctaatc gggttgtcag
     8461 cggcaaggag ttcattgaga taaccccata ctccggcgtg ttcccgacac tcctgaggca
     8521 gcaccaacat catggaacca tcatcgcggc tcaacagttg gctgttaaac agataggtag
     8581 acaccgcatc cgacacggaa acctgagttg ccggaacttc tatcgccata aaaccgttca
     8641 cccgcgtgcc agtaactgtg actggcgagc gaacgcctgt tggtggcaaa acagcacctg
     8701 gcggttactc acggcaatca cgtcattatg aaaaacgctc tggtcgataa cgtccgggtt
     8761 ttgctgggcg aaaatcacct gttggggatt cacctgatta agccttgcca ccgcctcgct
     8821 ggcttcgcga gtctgtcgcg ccggataacg ggaaggccgg gtatcattgc cttcttctcg
     8881 cccgtagaca aaaagtagca tacccggttc accgtaatga ccgccgagac gattgtggtt
     8941 tgccgccccc tcatcaccga gcaacgctac ctgcggcaac gccgaatgga cgctaaattt
     9001 ctcttcgtcc ttaaaaatcg cttttaacag cgattcagtg acgggcgctt ccagcgaacg
     9061 gtgaaattta ttgttcaggt tggcaacggt gagatgcact ttgccatcca gcgtatcggc
     9121 agatggcgcg atcgttgccg cattggctac ccacattggc gaagcggaac tgacgctgga
     9181 aagccagtgc ggtgcctggc gtgcaacttt ttccagtacc tgctcatcgc taccactgaa
     9241 tcccaactga cgcagcaccg gaataaacgg acgctcgtgc ggcggaatca cggcctgggg
     9301 gaatcccgca tcggcaaggg ctttcatttt cagtaagccc tgttttgccg ccagtcgcgg
     9361 gttagacacc tgaaaacggt gacgggtaga ggcttcatta ccaaacgaca ggcccgcgta
     9421 atgatgcgtc agccctacca gcccgtcgaa attaacttcc caggcgttca tcgcaccacc
     9481 tcatcggaaa aatccagccc ggggttaagc gtggcgggca atgttaacga gtccgactcc
     9541 aggctcgcca taggccatgc gcagtaatct gcggcatacc aggcgctggg gcgatggtta
     9601 ccggaagcac caatgccgcc gaatggggcg gtactggcag caccagtaag cggtttgttc
     9661 cagttaacaa tccccgcccg cgcctccagc aacagttgat cgaatttttc ccgctcgggg
     9721 gaaaccagac cgcaaaagag tccgaagcga gtgttgttcg ccatccgaat cgcctcatca
     9781 aaagtatcat aacgccagac gcgcaaaaac ggcccgaaca cctcttcatc cggcacaccg
     9841 ccaacgcccg tcatttcaat gatccctggt gtcagcaacg atgtccctgc ttgtaataag
     9901 cgcggcgcaa gcagggttcg tccgcccatc gcttccagtt gctgccaggc agtaaccacc
     9961 tgctgtgcgg cctgttcaga aatcagcccg ccaataaacg gctgcggttc gtcatcccag
    10021 ttgcccggcg ttaatcgctg gctgacggca accagactag caagaaacgc atcgccctgc
    10081 gctccgcttt tgagcaataa acggcgggcg caggtgcagc gttgcccggc tgtgacaaac
    10141 gccgactgaa tggtcagatg gacagccgcg tcgatatccg ccacctcatc gataattagc
    10201 ggattattac cgcccatctc aagggcgaga attttctccg gctgaccgga gagctggcga
    10261 tgcagctggt agccggtatt ggcgctaccg gtaaacagca aaccgtcgag atcctccagc
    10321 gcactcagcg cctgacccgt ttcacgcccg ccctgcacca ggttcagcac gcccggcggc
    10381 aagccagcct gctgccataa acgcattacc gcttcgccac tccacggtgt cagttcgctg
    10441 ggtttaaaga taatggtgtt acctgccagc aatgccggaa cgatatgtcc gttcggcaaa
    10501 tgaccaggga aattatacgg cccaaacacc gccagcacgc cgtgcgggcg atgtcgcagg
    10561 ctcgccgcac cgtccggcat ttcactacgc tgctcgccgg tacgaacgtg atacgcctta
    10621 attgatatcg cgattttatt gatcatcgcc gtcacttcgg ttgccgcttc ccagcgcggc
    10681 ttacccgttt ctctggcaat aatcgcggtt aattcggctt tattgctttc cagcaatccg
    10741 gcaaagcgtt cgacaacgac ctgacgttca gccaatgaga gccgcgccca gcgcggaaac
    10801 gccgcacggg ctgcccgaca agcctgttcg acctgagcgg catcggcatc attgccttgc
    10861 cataacacct cgcccgatac cggattacgc ttcacacgcg atgcgccctg gcccgttatc
    10921 cagtcaccgt taatccataa agtcatgctg ttttctcctc tgcgcacagg cgcaccagac
    10981 gaacgcgatc ccctgcgtgg catttgaggg catccagttg tgcggcggtt aaaatcaaac
    11041 gctcggttgc cggatcggta cgcgccagca ccacgcggaa atggtgataa ttttcattgg
    11101 cgaccaggca ggccggaaaa tcgccctgcg caggctgccc ttctgccact tccaccagcc
    11161 gacttttacg gatggcgcgc acgcggtcga tgtcacactc aagcgttggc ccgccgtcga
    11221 agatgtcgat atagttgcgg taacgaaaac cttctttctc cagcaccgcg cgggcaggcg
    11281 cggtttgcgg atgtacctga ccgatgacgt cctgggcttc ctgggataaa aagtgggtat
    11341 agatcggatg tttcggcatc agttctgcaa taaatgcctt ttgcccggtg ccgcagagaa
    11401 aatcggcgcg gctaaaatcc atcgaaaaga agcgtttacc gaggctttgc cagaacggtg
    11461 aatagccgtg ttcgtcaatc accccgcgca tttcagcaac cactttgtca ttaaacttgt
    11521 cgcgaaaagc cgccataaac ataaagcgcg atttcgacag caaatagccg ttgccctctt
    11581 tgcgccagtc cgggtcgaga aacagcgtgc acagctcgct gctgccggtg tgatcgttac
    11641 taagaaacag cgtcggcaat gcgttataga cattcagctc ttttgaggcg tgaaccaacg
    11701 tgccgacgcg atagttgtac cagggatcgt tcagcccaac cgccacctca atagcgcaaa
    11761 tccccgccac ggtgcctgtc tcgctatctt ccagcacgaa cacatagccc tgctcgcttt
    11821 tgggcagttc accttgccag gttttgattg ccctttcgat acgcgccgaa agcgtggctt
    11881 cattggcggg aagcgacgtc aggccgccgc ccgttttgct ggcaagctgc atcagcgccg
    11941 agacatctga gcgctcaacg ggacggatga ccatcatgat gaacctcggc taacaaagtg
    12001 ttcgcaagca gctgcaaagc gatccagtcc ggtcgtcacc tcttcttcgc tgacattgag
    12061 cgcaggcgca aaacgcacca cgttgccacc cgcaatcagt accatcacgc ctgctttcgc
    12121 cgcttccaga gagatctgtt tcgcttgccc ggcgtaatcg gcattcagta cacagccaat
    12181 caacaaacct aagccgcgaa cttcactgaa caaaccatag cggtgattaa tagtattaag
    12241 acgctcaaca aaccagtcgt gacgctgttt aacgccatta agcatctctg gtgtgttgat
    12301 gagctccagc actttgcctg ctaccgccga ggccagcgga ttaccgccat aggtggtgcc
    12361 atgagtgcca acggtcatca cgcgggcgca ctcttcggtt gccaacaacg caccgaccgg
    12421 gaaaccgccg cccagcgctt tggcggtggt taacagatcg ggcgtcacgc cgtagtgcat
    12481 ataggcatac agctcaccag tgcgcccgac gccggtttgt acctcatcaa aaatcagcaa
    12541 ggcattgtgg cggtcacaca attcacgcag accttgtaaa aacgcgttgc tggctggcac
    12601 cacaccgcct tcgccctaga tgggctcgac aatcaccgca caggtggcgt cgtcaatcag
    12661 cgcgctggca gagttaatat cgttatatgc agcatgacga atatccgccg gcagtggcgc
    12721 aaaatcctgt gaataggctg gctgcccacc cgcactgaca gtaaacagcg tgcgaccatg
    12781 aaacgcattt ttgaacgcca cgatgccact cttatggctg ccgtagcggt cgtgagcgaa
    12841 tttacgcgcc agttttagcg ccgcttcgtt ggcttccgca ccggagttac aaaagaagac
    12901 gcgatcggca aacgtggcgt cgatcaattt tttcgccagt cgcagtaccg gctcgttggt
    12961 gtaaccgttg ccggtatgcc agaacttact cgcctgttcg ttcagcgctt cacgcagttc
    13021 cggatgcgca tggcccagcg cgttcaccgc aatgccaccc gcgaagtcga tatactcttt
    13081 cccctgctga tcccacaagc gcgaaccttc gccacgtacc ggtataaagg gtgccggagc
    13141 gtaaacaggt atcatccatt catcaaagtt ttcacgcgta attggctgag acatagcgac
    13201 ctctacagta aataattcgt tatttgtatg ttaacaataa gtaatgtttg cgctgtaaat
    13261 gtagattgca gggttcgtgc cagccagtga taaaagtgca taaacggcgg aggctaactg
    13321 gaaatcaagg agttataacc aaaccatatg catttaaagt gcatataaag tgaatacgtt
    13381 tgcgatgtgg gtgaataaaa agaataaaaa acgcaatgtt atgcagaagt aaaatataat
    13441 tctagaattg tgatcattga cgaaatttac tggaaattac tgcgccattc tgacgcagcg
    13501 cgcaccaaaa gcgggcattt tttgcgccat cgttgacatc attaacaacc atcgatcaaa
    13561 tcacttaaca acaggcggta agcaacgcga aattctgcta ccatccacgc actctttatc
    13621 tgaataaatg gcagcgacta tgaaatttgt ctcttttaat atcaacggcc tgcgcgccag
    13681 acctcaccag cttgaagcca tcgtcgaaaa gcaccaaccg gatgtgattg gcctgcagga
    13741 gacaaaagtt catgacgata tgtttccgct cgaagaggtg gcgaagctcg gctacaacgt
    13801 gttttatcac gggcagaaag gccattatgg cgtggcgctg ctgaccaaag agacgccgat
    13861 tgccgtgcgt cgcggctttc ccggtgacga cgaagaggcg cagcggcgga ttattatggc
    13921 ggaaatcccc tcactgctgg gtaatgtcac cgtgatcaac ggttacttcc cgcagggtga
    13981 aagccgcgac catccgataa aattcccggc aaaagcgcag ttttatcaga atctgcaaaa
    14041 ctacctggaa accgaactca aacgtgataa tccggtactg attatgggcg atatgaatat
    14101 cagccctaca gatctggata tcggcattgg cgaagaaaac cgtaagcgct ggctgcgtac
    14161 cggtaaatgc tctttcctgc cggaagagcg cgaatggatg gacaggctga tgagctgggg
    14221 ggtggtcgat accttccgcc atgctaatcc gcaaacagca gatcgtttct catggtttga
    14281 ttaccgctca aaaggttttg acgataaccg tggtctgcgc atcgacctgc tgctcgccag
    14341 ccaaccgctg gcagaatgtt gcgtagaaac cggcatcgac tatgaaattc gcagcatgga
    14401 aaaaccgtcc gatcacgccc ccgtctgggc gaccttccgc cgctaattta gcagctctcc
    14461 tggctcaaac tgggtcagga gaattaacct tgagaaaaat caacaaactg tcagtaatga
    14521 tttgttgcct gccgtccttt gttataccgt cactgcgttt ttagttgtct gaccacttct
    14581 ctattatcaa gtttgatata ggaaactcca cgatgaacgc tgagcgtaac tttctgtttg
    14641 cctgtcttat ttttgcgctg gtcatttacg ctatccacgc tttcggttta ttcgatctgc
    14701 tcaccgattt accccactta cagacactca ttcgccagag cggattgttc ggttatagcc
    14761 tctatattct gttattcatc aatgcccccc tctttctgtt accaggaagc atactggtga
    14821 tcgctggagg aatagttttt ggcccgctct tagggacaca actctcatta attgccgcaa
    14881 cgctggcctc ctcgtgctca ttcctgctgg cgcgctggct gggacgtgat ttactgctga
    14941 aatacgtggg ccatagccat accttccagg ccattgaaaa aggcattgcg cgtaacggta
    15001 ttgattttct tattctgacc cgcttaatcc cgttgtttcc ttacaatatt caaaattacg
    15061 cttacggatt caccaccatc gccttctggc cttataccct tatttcggca ctcacgaccc
    15121 tgcccggtat tgttatttat accgtgatgg caagcgatct cgccaatgaa ggcattacgc
    15181 tgcgctttat tttacaactc tgtctggcgg gcctggcgct gtttattctc atccagctcg
    15241 caaaactcta cgcccgacac aaacatgtgg atctgtctgc ttcgcgccgc agcccactta
    15301 ctcacccaaa aaatgaagga tagaacgatg tcgcaacatt attcagtgtc atggaaaaaa
    15361 ggactggctg cactctgctt actggctgtt gccgggctta gcggctgcga tcaaaaagag
    15421 aatgcggcgg caaaagtgga atacgacgga ctttcgaaca gccaaccgct gcgcgtcgat
    15481 gccaataacc atacggtaac catgctggtg caaattaatg gtcgtttcct caccgacgac
    15541 actcgtcacg gtattgtgtt taaagatggc tccaacggac ataaatcgct gtttatggct
    15601 tatgcgcccc cgaaagcatt ttatgaagcc ctgaaagagg caggtggtac gccgggcgaa
    15661 aacatgacga tggataataa agaaacgact catgtcacag gcagcaaact ggatatttcg
    15721 gtcaactggc aaggggcggc aaaagcgtat tccttcgatg aagtgattgt tgacagtaat
    15781 ggcaagaaac tggacatgcg ctttggcggt aatttaacgg cagcagaaga gaagaaaacg
    15841 ggttgcctgg tgtgtctgga tagctgcccg gtcggcatcg tcagcaatgc aacatacact
    15901 tatggtgcgg tggaaaaaca cggcgaagtg aaattcaaag gcaatgcctc agttctcccg
    15961 gcggataaca cgctggcaac ggttaccttt aaaatcaccg aataaagcca agataaagga
    16021 tgatgaagat acaatcgcgt aaaatctggt actaccgtat aaccctcatc atcctgttgt
    16081 tcgctatgct gctcgcatgg gcgctgcttc ccggcgtcca tgagtttatc aatcgcagcg
    16141 ttgcggcgtt tgccgccgtg gaccaacagg gtatagaacg ctttattcag tcttacggcg
    16201 cactggcggc agttgtctcg ttcttgttga tgattttaca ggccattgct gcaccgctac
    16261 ctgcgttttt gatcaccttt gccaatgcgt cgctgtttgg cgcgttctgg ggcggcttgc
    16321 tgtcgtggac cagttcgatg gccggcgcgg cgctgtgctt ttttatcgcc agagtgatgg
    16381 gccgcgaagt ggtggaaaaa ttaaccggca aaaccgtgct tgacagtatg gacggctttt
    16441 tcactcgcta cggcaaacac accatcctgg tctgtcggtt attgcctttt gtccctttcg
    16501 atccaatcag ctatgctgcc ggtctgactt caatacgttt tcgctcgttt tttatcgcca
    16561 ccgggcttgg tcaattaccg gcgactattg tttattcctg ggcaggcagc atgttaacag
    16621 gcggtacttt ctggtttgtc accgaactgt ttattctgtt tgccctgacc gtggtgattt
    16681 ttatggcgaa gaaaatatgg cttgagcgcc agaagaggaa tgactgatgg gtttaccgcc
    16741 gcttagcaaa attcctttca ttttacgtcc gcaggcgtgg ctgcatcgtc gccattacgg
    16801 cgaggtgcta agcccgattc gctggtgggg gcggatcccg tttatctttt atttggtgtc
    16861 aatgtttgtt gggtggctgg agcgcaaacg ctcaccgctc gatccggtag tgcgatcgct
    16921 tgtcagcgcg cgcattgcgc aaatgtgcct gtgtgagttt tgcgtggata tcaccagtat
    16981 gaaagtcgcc gagcgtaccg gcagcaccga taaactgctg gcagtggctg actggcggca
    17041 aagcccgctc tttagcgatg aagaacggct ggcgctggag tacgccgaag ccgcaagcgt
    17101 cacaccgcca acagtcgatg atgccctgcg tacccgactg gctgcgcatt ttgacgctca
    17161 ggcgctcacc gaactgacgg cattgatcgg cctgcaaaac ctgtcggctc gttttaattc
    17221 tgccatggac attcccgctc aggggctgtg ccgtattccg gaaaaacgtt cttaaggagg
    17281 gaagatgcgc cattgtgggt ggttgctggg attgttatcg ctgttttctt tggcaacaca
    17341 tgccagtgac tggcaggaaa ttaaaaatga ggccaaaggg caaaccgtct ggtttaacgc
    17401 ctggggcggc gataccgcaa ttaaccgcta tctcgactgg gttagcggcg agatgaaaac
    17461 ccattacgct ataaacctga agattgttcg tctcgcggat gccgcagacg cggtgaagcg
    17521 cattcagacc gaagctgctg ccggtcgtaa aacgggcggc tcggtggatc tgctctgggt
    17581 gaacggcgaa aacttccgca ccttaaaaga gtaatgccag tcagttaagc aactgactgg
    17641 catt
//
LOCUS       DAJTSA010000084        17385 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000084 DAJTSA010000000
VERSION     DAJTSA010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.084"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(41..772)
                     /gene="rlmB"
                     /locus_tag="OO369_002978"
     CDS             complement(41..772)
                     /gene="rlmB"
                     /locus_tag="OO369_002978"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR5983173.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(952..3393)
                     /gene="rnr"
                     /locus_tag="OO369_002979"
     CDS             complement(952..3393)
                     /gene="rnr"
                     /locus_tag="OO369_002979"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR5983174.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3432..3857)
                     /gene="nsrR"
                     /locus_tag="OO369_002980"
     CDS             complement(3432..3857)
                     /gene="nsrR"
                     /locus_tag="OO369_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR5983175.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4062..5360)
                     /gene="purA"
                     /locus_tag="OO369_002981"
     CDS             complement(4062..5360)
                     /gene="purA"
                     /locus_tag="OO369_002981"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR5983176.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5464..5661)
                     /locus_tag="OO369_002982"
     CDS             complement(5464..5661)
                     /locus_tag="OO369_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR5983177.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5743..6747)
                     /gene="hflC"
                     /locus_tag="OO369_002983"
     CDS             complement(5743..6747)
                     /gene="hflC"
                     /locus_tag="OO369_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR5983178.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6750..8009)
                     /gene="hflK"
                     /locus_tag="OO369_002984"
     CDS             complement(6750..8009)
                     /gene="hflK"
                     /locus_tag="OO369_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR5983179.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8095..9375)
                     /gene="hflX"
                     /locus_tag="OO369_002985"
     CDS             complement(8095..9375)
                     /gene="hflX"
                     /locus_tag="OO369_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR5983180.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9451..9759)
                     /gene="hfq"
                     /locus_tag="OO369_002986"
     CDS             complement(9451..9759)
                     /gene="hfq"
                     /locus_tag="OO369_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR5983181.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9845..10795)
                     /gene="miaA"
                     /locus_tag="OO369_002987"
     CDS             complement(9845..10795)
                     /gene="miaA"
                     /locus_tag="OO369_002987"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR5983182.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10788..12635)
                     /gene="mutL"
                     /locus_tag="OO369_002988"
     CDS             complement(10788..12635)
                     /gene="mutL"
                     /locus_tag="OO369_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR5983183.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12645..13982)
                     /gene="amiB"
                     /locus_tag="OO369_002989"
     CDS             complement(12645..13982)
                     /gene="amiB"
                     /locus_tag="OO369_002989"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR5983184.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14001..14462)
                     /gene="tsaE"
                     /locus_tag="OO369_002990"
     CDS             complement(14001..14462)
                     /gene="tsaE"
                     /locus_tag="OO369_002990"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR5983185.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14434..15981)
                     /gene="nnr"
                     /locus_tag="OO369_002991"
     CDS             complement(14434..15981)
                     /gene="nnr"
                     /locus_tag="OO369_002991"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR5983186.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15980..17119
                     /gene="queG"
                     /locus_tag="OO369_002992"
     CDS             15980..17119
                     /gene="queG"
                     /locus_tag="OO369_002992"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR5983187.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17102..17155)
                     /locus_tag="OO369_002993"
     CDS             complement(17102..17155)
                     /locus_tag="OO369_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983188.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3942 a         4959 c         4367 g         4117 t
ORIGIN      
        1 aaccatccaa atctggatgg cttttcataa ttcagaggaa ctagctgcgc tgacgaaccg
       61 cttcaaataa gcaaattccg gttgcaaccg aaacgttcag ggaagaaaca cttcctgcca
      121 ttgggatgct gatcaactca tcgcaatgtt cacgggtcag gcgacgcata ccttcacctt
      181 ccgcgcccat caccagcgcc aggcgtccgg tcattttgct ctgatagagt gtatgatccg
      241 cctcgcctgc cgtaccgacg atccagatat tctcttcctg caacatacgc atggtgcgcg
      301 caaggttagt cacccgaatc agcggaacgc tttctgccgc gccgcaggct acttttttcg
      361 ccgtagcgtt gagctgtgcg gagcgatctt tcggcacaat caccgcatga acgcctgcgg
      421 cgtccgcact acgcaggcac gcgccgaggt tgtgcggatc ggttacgccg tcgaggatca
      481 gcaggaacgg ttgatcgagc gaagcgatca gatccggcag atcgttttcc tgatactgac
      541 gtcctggctt cacgcgggcg ataatgccct gatgcacggc accgtcgctt ttctcgtcga
      601 gatattggcg gtttgccaac tggataacca cgccctgaga ctcaagggcg tgaatcagcg
      661 gtaacagacg tttatcttca cggcctttta aaataaagac ttcctgaaaa cgttcagggg
      721 cgcgctccag cagggcctgc actgcgtgga tgccgtaaat catttcgctc attaatgtac
      781 tcgttgttta cgttttgcct gatgcactac gtttatccgg cctacatgat ctctgcaata
      841 tattgaattt gcgtgattat gtaggccgga taaggcgttc acgccgcatc cggcatgaac
      901 aaagcgcatt ttgtcagcaa tctaaccctc ttcttttaaa gagggtattg atcactctgc
      961 cacttttttc ttcgccgcac gcttcgcttt ggtcgctgcg gctattttct gcgttttcgc
     1021 cgatggcttt ttcgcttttc tcgcgtcttt cttcgccgct ttcggcttcg tttttttctc
     1081 accgcggaag gcgctgtctg gctcaaagtt taccttttta ccgacctgac aacgcttgcc
     1141 gccttttttg cctgcatcgc cttttttcgc tttctcgcgc gccgttttac cgacgttgcg
     1201 cggtgcgcgt tcactggaga tcagactaaa gtcgattttg cgctcgtcca tattaaccgc
     1261 ttcgacgcga acttccacgc gatcgcccag gcgataagtc tggccgctgg attcccccat
     1321 caggcgttgc cctacctggt caaagcgata gtagtcattg tccagcgaag agacatggac
     1381 cagaccatca atgaacaagt cgtccagacg gacgaagaag ccaaagccag tgacgctgga
     1441 aattacgcct ttaaagacgt tacctacctg gtcgagcatg aagtcacact tcagccagtc
     1501 agcgacatcg cgcgttgctt cgtcggcacg acgttccgcc atcgaacagt gctgacccag
     1561 ttgcaacatc tcttccatcg aataatggta gccgccggtt tcagtggtgt tgccctgatg
     1621 cccctgctct ttcgccagca gatatttaat ggcgcggtgc agcgtcaggt caggataacg
     1681 acgaatcggc gaagtaaagt gcgcatagga ctgcaatgcc agaccgaagt gaccacggtt
     1741 ttctggatcg taaatcgcct gtttcatcga gcgtagcagc atggtttgca gcatttctgc
     1801 atcaggacgg tcggcaaccg actccagcag ttccgcgtag tcacgcggtt ccggcttgtt
     1861 accacccggc agctccagcc ccagctccgc cagcactgaa cggaaagagg taatcgcttc
     1921 ggtgctcggc ttgtcgtgaa tacggaacag tgccggttct ttcgctttct caacgaaacg
     1981 cgccgccgag atattcgcca gaatcatgca ctcttcaatt aatttgtgcg cgtcgttacg
     2041 ctgggtctgt tcgatacgtt caatacggcg ttcagcgttg aaaatgaact tcgcttcttc
     2101 gctctcaaat gagatcccac cgcgttcttc acgggcttta tccagcactt tatagaggtt
     2161 atgcaactct tcgagatgct taaccagcgg ggcgtactgc tcgcgcagat cctgatcgcc
     2221 ctgcagaata tgccagactt tggtgtaggt cagacgcgcg tgagagctca tcaccgcttc
     2281 gtagaatttg tagcccgtca ggcggccttt cgacgaaacc gtcatctcgc acaccataca
     2341 caggcggtct acctgcgggt tgagcgaaca caggccgtta gagagcactt ccggcagcat
     2401 cgggataacc tgcgaaggga agtacaccga cgtgccacgg ttacgcgctt ctctgtccag
     2461 cggcgttggc ggacgcacat agtagctgac gtcggcaatc gcgacccata aacgccagcc
     2521 gccgccgcgt tttttctcgc agtaaactgc atcgtcaaag tcacgggcgt cttcgccatc
     2581 aatggtgacc agcggtaaat cgcgcaaatc gacacggccc gcttttgctt cttccggcac
     2641 ttcttctttc agccccgcaa cctgttgctc aacagcctgc ggccagatat acggaatttc
     2701 atgggtacgc agagcgatat caaccgccat gccggtgccc atattgtcgc ccagcacttc
     2761 gacgatttta cccaccgctt tggtgcggcg agtcggacgc tgagtcagtt cgactacgac
     2821 cacaaagccc atccgcgcgc ccatgatctg atcgggcggg attaagatat cgaagctcag
     2881 acggctatcg tcaggaacca caaagccgac gcccgcttcg gtaaagtagc gaccaacaat
     2941 ctggctggtt tttggcacca gtacgcggac aatacgcgct tcacgacgac ctttacggtc
     3001 agcacccagc ggctgcgcca gcacctgatc gccatgaatg caggttttca tctgctcgct
     3061 ggagagatac aaatcatctt tacgcccttc aacccgcaga aagccgtagc catcacggtg
     3121 accaataacg gtacctttca ccaggtcgag gcgttccggc agcgcatagc actgacgacg
     3181 agtgaagacc agttgaccat cgcgctccat cgcgcgcagg cggcgacgca ggccttcaag
     3241 ctgctcttcg ccttcaatgt gcagttctac cgccagctca tcacggctgg ccggtttttc
     3301 acgtttggtt aaatgttcga ggataaattc ccgactaggg atgggattcg cgtatttttc
     3361 agcttcgcgt tcctggaaag gatcttgtga catctcggtt cctccgttgt catctctgat
     3421 gaagattttc gtcactccac cagcaataat ttataaagcg gttgattctc ttcaaccaaa
     3481 tcggcaagcg tgtagttatc cagttccgta agaaaacttt gcacggcctt agaaagcgcc
     3541 tgtttcaacc gacaggcagg tgtaatgtgg caaaactcac tgctgcaatt caccagcgat
     3601 aagggctcca gctcgcgcac cacatcacca atacgtatcg cactcgccgg tttacccagg
     3661 cgaatgccgc cattttttcc acgaacagca gtcacgtagc cggcacgact aagttgattg
     3721 attattttga ccatatgatt acgggagacg ccgtagacgt cagtcacttc agaaatactg
     3781 gtcatccgcc cttctggcaa tgacgccatg tagatcagcg cacgtaatcc gtaatcagtg
     3841 aaactcgtta actgcacatc aacctcaaaa gggaaatcgg gaaaaaatac atttatattg
     3901 atgataaacc agccacaagc tgtgtcgcta atttatttca gttcggggaa ggaaaaaagc
     3961 gaggatttaa cactgtgtcg gatagcgtaa tggcttaacc gacacagcaa tgtatcacac
     4021 tgttagcccg gcaggcaaaa tatctgccag gcgtaccaga attacgcgtc gaacgggtcg
     4081 cgcagaatca tggtttcagt acgatccgga ccggtagaga tgatatcgat cggcacaccg
     4141 gtcagctctt caatacgctt gatgtagttc agcgccgcct gcggcaggcc gctacgatct
     4201 ttcacgccga aagtggattc agaccagccc ggcatggttt cgtaaatcgg ctctacacct
     4261 ttccagtcgt cagctgccag cggagtggta gtcacttcgc gaccatccgg catacggtaa
     4321 gccacgcaga gtttaacctc tttcaggcca tccagaacgt ccagtttagt caggcagaag
     4381 ccagacaggg agttcagctg taccgcacga cgaacggcaa cggtgtccag ccagccggta
     4441 cgacgacgac gacccgtagt tgcaccgaat tcgttaccct gcttgcagag gaactcgcca
     4501 gtttcatcaa acagttcggc cgggaaagga cctgcaccta cacgagttga gtaagctttg
     4561 aggataccca gaacgtaatc aacataacgc gggcccaggc cggaaccggt cgccacgcca
     4621 ccagcagtgg tgttggaaga agttacgtac ggataagtac cgtggtcgat atccagcagc
     4681 gtaccctgcg caccttcgaa catgacgaaa tcgccacgct gacgcgcctg gtcgagcagg
     4741 tcagaaacgt caaccaccat agaagtcagg atgtcggcaa cagccatcgt atcatccaga
     4801 actttctggt aatcaaccgc ttcagctttg tagtagttaa ccaactggaa gttgtgatat
     4861 tccatcactt ctttcagttt ttcagcgaag gtttctttgt cgaaaaggtc gccaacacgc
     4921 agaccgcgac gtgccacttt atcttcataa gcaggcccga taccacgacc ggtggtgccg
     4981 atcgctttcg cgccacgcgc tttctcacgc gcgttatcca gagcaacgtg ataatcaagg
     5041 atcagcggac atgcttcaga cagcagcaga cgctcacgaa cggggatgcc acggtcttcc
     5101 agttctttca tctctttcat cagcgcggcc ggagacagca caacaccgtt accgatgatg
     5161 ctggttacat tctcgcggag aatacctgat ggaataagat ggagaacggt tttttcaccg
     5221 ttgattacga gagtatggcc tgcgttgtga ccgccctggt agcgtacaac atatttagcc
     5281 cgttcagtca gaagatcgac gatcttacct ttaccttcgt caccccattg ggtgcccagt
     5341 acgacgacgt tgttacccat ttttcaaaat caccgtttgc ttaaaaatgg attctaccat
     5401 cgctttttca gagttacagc actttttgca ctcaaaaatg acctcgatca gtctgttttt
     5461 tgttcagcca atcgttttcc tcaacatgta gtagaccaca acgcccgcaa ccacaagtcc
     5521 accgccaaaa cgacgtaaaa tattatcggg caaattggtc atcgcagaga tcatcttctt
     5581 ccatgccttc gggtaaagca tcggccctaa accttccagt accaaaacca gggcaagcgc
     5641 cagccagatt gtcgaattca ttcattatcc ttatagaaaa agaaaaccac cgacatccct
     5701 gaggatgcgg tggctttatt gacctgtacc gcagtcgtta tattaacgcg ttgcggaagt
     5761 cggcgtcttc atgtagcgga agaaatcgct atccgggctc atgaccatca cgtcctgatt
     5821 gccagagaag ctgttctcat aagcacgcag gctacggatg aatgcgtaga agtccggatc
     5881 tttactgaat gcatcagcaa acagtttggc tgcttcggca tcgccttcac cacgcataat
     5941 gcggccctga cgctcagctt ctgccagcgt tctggtcact tcatagtcgg cagtcgcgcg
     6001 cagtttttcc gcttcttcct gaccttgtga acggtgacga cgcgctaccg cttcacgctc
     6061 ggcgcgcata cggttgtaga tcgcttcaga cacttcggtc ggcaggttga tctgcttgat
     6121 acgcacatcg acaacttcaa tacccagcgc cgccatactg ttcgggttga tgaccggaac
     6181 tttgcccttc gtctcagccg ttacgcgctc tgccgcttcg gcaatggcgt tatctgctgc
     6241 cggggtagta acttcatctt ctgtacccgc agaaccggag ttcagcgcgt cacgtacttc
     6301 gagggtcaga cgaccacggg aatcggtgac gatatctttc acgtccaggc gaccaatttc
     6361 agaacgcaga cggtcagaga acttacgttt caacagcact tccgcttgcg aaatgtcgcc
     6421 accacccgtt gccaggtagt aacggctgaa atcgctgatg cgccatttga tgtaagagtc
     6481 gacgatcagg tctttcttct ctttggtcac aaagcggtcg gcctggttgt ccatggtctg
     6541 aatacgtgcg tcgagcattt tcaccgtttc aatgaacggt atcttgaaat gcagaccagg
     6601 ctcataaacc aaaggtttgt tgtcatcgtc acgcagtacc ttaccaaaac gcagcgtaat
     6661 accgcgctca ccttctttga cgacaaagac agacatgtaa agcactacca gcacgatgat
     6721 gataatcgcg ataactgact tacgcatcgt tattccccct gacgctggta gtcgttacgc
     6781 tgcgcgttgg cgcggcgttg gtccataata tcgccctgac tggtggacga cgtgttgctt
     6841 gctccactgg ttgtggagga agaggctggc ggcagacgca gcagattgct ggcaccgtta
     6901 tcgctcttcg ccgcaggggc gttaccacct ttcagcatct ggtctaacgg cagaaccatc
     6961 aggttgccac ctttatcgtt aaccagcact ttgcgggtgt tacccaacac tttttccatc
     7021 gtctcgatat acagacgctc gcgagtaatt tccggcgcgg ctttatattc cggcagaagt
     7081 ttagcaaagc gcgccacttc accctgagct tccaggatgg tctgggcctt gtacgcacgc
     7141 gcctcttcga ggatacgttg cgcctgaccg ttcgcacgcg gctgaacttc gttggaatac
     7201 gcttctgctt cacgaatgta ttgctgttcg ttttcacgcg cggcaatcgc atcgtcaaac
     7261 gccgctttta cttcttccgg cggacgagca gcctggaagt tgacgtccag cagcgtgata
     7321 cccatgtcat acggacgaat cgtctcttcc agttcgcgct gagtatcgct acgaatcacg
     7381 gtacgacctt ccgtcagaat gcggtccatg gtgtatttac cgataactcc acgcagggcg
     7441 ctgtcggtag cctgacgcag gctgtcatcc gggctggtca cgctatacag atatttttcc
     7501 ggattggtga cgcggtactg cacgttcatc tcaacgcgca ctacgttctc gtccgacgtc
     7561 agcatcacac cagaagcggc cagttcacgc acggcttcca cgttcaccgg tttgacttcg
     7621 tcgataaacg tcggtttcca gttcagaccc ggctcaacca gatggctgaa tttaccaaag
     7681 cgtgttacca cgccgcgttc ggcttcttta atggtataga aaccactggc cgcccagata
     7741 atgacaatcg ctgccgctgc gatggtaacg acacgaccgc caagctgcgg gcgcgggcct
     7801 tgcgatgaac tgccaccgcc agatccggtg cctttaccgc cgcccagacc accgagcttt
     7861 ttgctcagtt tgcggaagat atcatctaaa tcaggtggcc cttgatcgcg accgcctttg
     7921 tttccatttc cctcagagtt gccgccaggt ttgctgcttc cccacgggtc gcggtcttgt
     7981 ccgttattac cgggctgatt ccacgccatg tttgtgctcc atatttgtta tgcggtgatc
     8041 cctgttgtct ttcgacgcca gaggatatga ctccacgctt cagacgctac gccgttagat
     8101 caggtaatcg atcaacgccg gttcttgttt acagaggcga cgccagtcaa cgatcggcat
     8161 acgaacttgc agacttacgc tgccgtcctc ctccatccac tctttttcta ttgcctgaag
     8221 ctgataaaaa cgactcctca gacgcccttc ctgcggtggc agacgcaatg tatgctgcgc
     8281 cacctcgccg gaaagccgct ccgtcaaagc ctgaaaaagc tgtggtatcc ccgctccggt
     8341 ctgtgcggaa agccagacac ggatcggttt gttctcttcg tcccgatcaa tacgcggttc
     8401 gaaatcttcc agcatatcga tcttgttcat caccagcagg gttgggatct cgtgagcgtc
     8461 gatctcttca agaaccgtat tcaccgcttc gatgttttct tgtacacgca catccgccgc
     8521 atcaatgacg tgcagcagta atgtggcttg ccgcgtctct tgtaacgtgg ctttaaatgc
     8581 cgccaccaga tcgtgcggca ggtggcgaat aaaccctacg gtatctgcaa gtacggtttc
     8641 accgacatct gcaacgtcaa tacgccgcaa cgtcgggtcg agggtggcaa acaactggtc
     8701 tgccgcgtag acccgcgctt cggtgatgcg attgaaaagg gtagatttac cggcgttggt
     8761 atatcccacc agcgaaacag taggaacgtc ggctttgata cgcgattgcc gcccctgctc
     8821 acgctgcttt tcaactcttt ccaggcgcga ctgtatctgc acgatgcgat tacgcaacaa
     8881 acgacggtcg gtttcgagct gggtttcacc cggaccacgc aaacctatcc cgcctttctg
     8941 tctttcaagg tgggtccagc cacgcaccag gcgcgtagcc agatggcgca gctgcgccag
     9001 ctcaacctgc aacttaccct catgggtacg cgcacgttgg gcgaaaatat ctaaaataag
     9061 gccggtgcgg tcgataacac gacactcgca caaacgctcc aggttacgct cttgcgccgg
     9121 gctcagggca tggtcaaaaa gaacgaccga agcacccatc gctttgacag cttccgcaat
     9181 ttcaactgct ttaccttcac ctacaaaata ctttgggtgc ggcgctttac ggctaccggt
     9241 aatcacctgc aatgcttcga caccggcgga agagaccaga gattcaaact cctggaggtc
     9301 ttccatatct ttgtcttgcg taaaatagat gtgtaccagt accgcctgct caccagcatc
     9361 ataacggtca aacaagcgta taaccctcta aatagatcag cggggaacgc aggatcgctg
     9421 gctccccgtg taaaaaaaca gcccgaaacc ttattcggtt tcttcgctgt cctgttgcgc
     9481 ggaagtattc tgcgcgctgc taccatgatg gtagttactg ctggtaccgc caccggcgtt
     9541 gttactgtga tgagaaaccg ggcgagatgg gacaacagta gaaatcgcgt gcttgtaaac
     9601 catctggctg accgtgtttt tcaacaggat cacgaactga tcaaaagact cgatttgccc
     9661 ttgcagctta ataccattca ccaaataaat agaaactgga acacgttccc gacgcagtgc
     9721 gttcaggaac ggatcttgta aagattgccc cttagccatt ctctcttttc cttatatgct
     9781 tatttgtact ttgaaccttt cgattctgaa aaaattgcgc acgatacgtc tcaattgtac
     9841 acattcagcc tgcgatagca ccaacaacct gtaatacttc gtcacgcgcc tgttctggtt
     9901 tttcactgtc aagccagtga accccttccc aaccacgcag ccaggttatc tgccgcttcg
     9961 ccaactgtct cgtggcgcaa acacctcgat aaaccatttc atcgtatgag atttcgcctt
    10021 caaggtaaga ccacatctgg cgataaccca cgcaacgaat ggaaggcaag tccgtatgca
    10081 aatctcctcg ggcaaaaagc gcccggactt ctgcttcaaa acctgaagcc aacatctgat
    10141 gaaaacgctg ctcaatgcgt tgatggagca gttcacggct cgccggggcg atggcgaact
    10201 gatgcacctg atacggtaga gcgtctcctg acgtttgcgt cagttccgtt aaagttttac
    10261 ccgaaatgaa aaaaacttcc agtgcccggg aaagcctttg tggatcattt ggatgaatcc
    10321 ttgctgccgc aaccggatct acctcctgaa gttgacgatg caatgactcc caaccttgct
    10381 ctgccgcctg ttgctcaatt ctggcccgca cttccgggtc tgccgacggt agcggcgaca
    10441 acccttccag caatgccttg aaatacaaca tcgtaccgcc cactaacagt gggatccgcc
    10501 ccgccgcggt gatatcggcc atttccgcca gcgcatcgcg gcgaaaatca gcagccgagt
    10561 aagcctgcga cggatcgcga atatccagca atcggtgcgg cgcggcgagt aactcttcag
    10621 cgttcggctt cgccgtcccg atatccatcc ctttgtaaat aaggacagaa tcaacgctta
    10681 tcaactctac tggtaaaatt ttacgcagct caatggctaa cgccgtttta ccggaggccg
    10741 tcggccccat caaaaaaatc gccttaggca ggctcgcctt actgatatca ctcatctttc
    10801 agggctttta tcgccggatg taaatcaaca gattgtaaca gaccacccgg cggcgttttc
    10861 acaagttgcg gacataaccg ttccacgtcc gccagcaggg ttatggcctg tgccattgac
    10921 cactgcgcat gttcgctcat cagatttcgt gcaatccact gcgcaatatt gccaggttcg
    10981 aatacggact gcttcgccag gtagcctatc agttcaggaa tcaagatttg taaattttgt
    11041 tggcgtaagg gtaaaggcac ggccctgatg gtcacatgct gtgcatctga ctggaaatca
    11101 atacccaatt ccgctagggc agactgcgct ttttctaatg ccgatttttc ttcgccagaa
    11161 acttttagcc gcaacggaat cagcagcggc tgggcgcaaa cgggcgcttc acccggcgtc
    11221 aattgtgcct gacgcagcca acgttctgcc actggcaagg ataaaagtga aatgttgcca
    11281 tcgcgctcca gcaacgcaca gtcggaatgg acgatagtca gtacccgacc aaaactctga
    11341 ctgttcgccg caagtgcagg ttcctgcggt tccggcgctt ttggtttttg catcggcgcg
    11401 ggcgtttgca aaagctggcg atacacttca ccttgctgtt tctggtagcc tggctgcgca
    11461 ttcggccagg gggcagccgg acgactgcct gaggcgggcg caggagagta gcgcggagct
    11521 accggctcac gagctgccgg ttctgcaaag tgattgcgcc ccgccgccac gcggttttcc
    11581 gggatggcac gcggtgcggg ttgtggttca tcgtccagcg gtagcggcgt ttccagttgc
    11641 tgttgtagca cgctcagcac gccctgatag ataaaatcat gcaccagacg cgactgatgg
    11701 aaacgcactt cgtgtttggc ggggtgtacg ttgacgtcca cctgatgcgg gtcgatctcc
    11761 agatacaaca caaatgccgg ttgctgatcg gcccccagtt tgtcttcgca ggcctggcgg
    11821 atcgcatgat tgatcaggcg atcgcgcatc atgcgaccat tcacgtagca atactgaatt
    11881 tccgccagtg cgggcgtagt gtgatttgga tcggccaccc agccgcgcag ggtgagatcg
    11941 ccgtgttgcc attcaatcgc cagcgcttgt tcgagaaaag cggtgccgca aatcgcgcct
    12001 aagcgccgtt ctttttgccc gccttccggc actgcgcggt actgacgcac aattttaccg
    12061 ttatgcgaca ggttgatcgt gacgtcgaaa cgcgccagcg caatgcggcg gatgatttca
    12121 tcaatatggc taaattcggt tttctcggtg cgcaggaatt tgcgccgcgc cggggtgttg
    12181 tagaacagat ccagcacctc cagcgtcgtt cccaccggat gcgctgccgg tttaaccgtc
    12241 acgtccatgt cgcgtccttc ggcataggcc tgccaggctt cctgctgttc tgcggtgcgt
    12301 gaagtgagcg tcaggcggga aaccgaactg atactcgcca gcgcctcacc gcgaaagccc
    12361 aggctgataa tggcttcgag atcgtccaga gaggcgattt tactggtggc atgacgagcc
    12421 agcgccagcg ccagctcatc ttttttgata ccgcagccgt tatcacgaat gcggataagt
    12481 ttcgccccac cgcgttcgat atcaatatcg atacgcgtcg cacctgcatc gaggctgttt
    12541 tccactagtt ctttgactac cgacgcaggt cgctcgacca cctcacctgc ggcaatctgg
    12601 ttcgccagtt gtggcggtaa gacctgaatt ggcatcaatc gtccttagtt tggcagcgtg
    12661 cgatctggcg tcgtcaccgt actggcagtt tgtgccgttg caccctgcgg cgcagattgc
    12721 atcggatgcg caaggaagta attgcgcagg cctttgtaaa tggcttctgc cagctgttgt
    12781 tggtaatcgt cgctcgccag caaacgttct tcgctgttgt tgctgataaa accggtttcg
    12841 accagtactg atgggatatc cggcgagcgc agaacgccaa ggctggcgtg ttctggtcga
    12901 cgtttatgta tttcgccaat gcgttgcaac tgactgatca tactggtcgc tacatcatac
    12961 cctacccgct gggaatgacc gaactgtaaa tccagcaccg cctggcttaa ataggggtca
    13021 gactgactgt tcgccagcac atcacccgcc ccacccagca gctccgacag tttctcgtgc
    13081 tgctccagcc agctggccat ttcactgttg gcgcgacggt tagagagcac ccatacggaa
    13141 gcgccagtcg cactacggtt cggtgcggca tcagcgtgaa tcgacacgag gaaattggcg
    13201 ttttgcttat gtgccacatc actgcgcccc atcaccgaga taaagtaatc cccgtcacgg
    13261 gttaaaacgc ctttaaacat cggatcgtca ttgagcaaag tacgcaattt acgcgcgatg
    13321 gcgatggtga catttttctc ccgcgtacca ccggggccga tagcgccagg gtcctgaccg
    13381 ccgtgtccgg catcaatagc gatgataatt ttatcgccag tgttagccgt cgcgcgcgct
    13441 gccggacgcg ttaccgtatt actgctgata acacccgtag tgcggttact ttccgtttta
    13501 aacggattgc gcgccggttc gctgacgcgc ggtgcgacaa ccgcaggcgt ttcaacgcgt
    13561 ttcgcaacca cgggcggcgg aggaggcggt ggcggcacat cggcgttaat cgtaaagacg
    13621 acagtgtaat tgctgccatt ctgccgcttc accgcttcgg ttttaccgtt ttcggtaaga
    13681 tcgaccacca gccgtagcgt ttgtgcatct ttaggcgttc cagagcgaat cgccttcacc
    13741 agattattgc cgctgaacaa caacggcagt ccctgaatca tgcccgtttg tttgatatcg
    13801 agcgccacgg tgcgtttgct ttgatggcta aacgcataat caggatcgcc aataaaactc
    13861 aacgttatcc gcgcctgttg attaccgtta gaaacctgaa tatcagagag cgtcgcggca
    13921 cccaccggcg tgcacagcag cagcagcgtc gctaccaacc aatttctgat gcgatacatc
    13981 atcccgccac ctttcaaagg ttaaccggct aaacgcgcca gcaacaattc acccgcagag
    14041 gaaaccgcac tcacgcgcgc ctcacggcct tgtgcctgat aatcaatgtg tatttcgaca
    14101 tccgggtcag gaagaacacc tgtaccttgt tgtggccact ccaccaggca gatggcatcg
    14161 ttggcaaaat aatcgcggat ccccataaac tccagctcct cgggatcggc aaggcggtac
    14221 aaatcaaagt gatagaccat taagttgtcg agcgtatagg gttcgaccag cgtataagtg
    14281 gggcttttga cattaccctg atgacccaga gcctgtaaaa agccccggct aaaggtggtt
    14341 ttacctgcgc ctaaatcgcc atacagatag attacggttg cgccatcgca ggctttcgct
    14401 acccgctcgc ccaggtctaa tgttgcctgc tcatcaggga gcggaattac tcgattcatc
    14461 atggttttta tcagtcactt ccgggttaac aatacgctgt agcgtggaaa agagatcggt
    14521 tgccagcatc ccgcgcgttc caaaacgcgc cgccagtacg tcagctgccg caccgtgcgc
    14581 gacacagcct gcacaggctg catcatacgg cgacagtttt tgcccaagca atgcgccaat
    14641 aataccagag agcacatcgc ccatgccgcc gctcgccatg cctgcatttc cggcatcaat
    14701 aatgcctaaa gcgtcaggat gggcggcgac cacggttccg gcacctttca gcaccgctac
    14761 gccgccataa cgttgtacca gacgtttggc gcaatgtaag cggtcacttt caatttcagc
    14821 gacggaacag cctaacaacc gtgcggcctc gctaggatgc ggcgtgatca cgcgattgtg
    14881 acgcttatcg ggattgattg ccagcaggtt caatgcatcg gcatcccaca acatcggttt
    14941 gcgaaaattc tcaacttttt gcagggcttt tttcccccac tcttgctggc ccagaccggg
    15001 accaatcacc accacatcgg cccattccag gctttcggta agagagtcca tagtcagttc
    15061 atgcaccatc aattctggtc gtgcagtcag cagcggcgca atgttctcac tgcgggtcag
    15121 tactcggact aaaccagcac cagcacgtag cgccgcttcc cccgtcatac gaatagcccc
    15181 cgccgtgccg tgatcgccac cgataatcac cagccgcccg tgatcgcctt tatgcgaagt
    15241 cgggcgacgc ggtttcagcc agtgagaaag ttgttctgcc gaaaaccgct gaattttcgt
    15301 ctcctgacct gccagccagc tatccagccc cagtgagtca aaatgcagtt gtcctgtaac
    15361 atcccgcgct tttccagtga gcaagcctag tttcagcgca ataaaagtga tggtgtgatc
    15421 ggcgttgatc actgcgcctg gcgtagcgcc ggtttcagcc agcaggccgg aagggatatc
    15481 aaccgccaca atcggcgcag gatgggaatt agcgtggtcg attaactggc taatggattc
    15541 gcggggcgct tgctgcaagc cggtaccgag cagcgcatca acaatcagat ctaccgattc
    15601 gggccagaca atattcgaag catggatctc gccacccgcg tttaaccatg cttcgcgtgc
    15661 cagcgcggcc tcttccggca acggtttgtc gctctcctgg gccaacaacg tgacctcaat
    15721 gccgaccgct ttggccagtc gcgcgaccac gtagccatcg ccgccgttat taccatgacc
    15781 gcacagcacc agccagtggc gggcgtcagg atacgccgaa cgacacacct ggaatgcggc
    15841 ctcgccagcg cgaagcatca gctcatagag tgtgagcccc agcacatctg ccgcctcgcg
    15901 ttctccgcgg cggatatcgt cggcgtacca gacggtgtgt ggtatactta cggggttttt
    15961 cttcattgta tggtccgtca tgtcagagcc cctcgatctc aatcagttag cgcaaaaaat
    16021 taaacagtgg gggctggaac tgggctttca gcaggtaggt attaccgata ccgatctcag
    16081 cgagtccgag cccaaactgc aagcatggct ggacaaacaa taccacggcg aaatggactg
    16141 gatggcacgt cacggtatgc tgcgcgctcg cccccatgag ttattacccg gtacgctgcg
    16201 tgtaatcagc gtgcggatga attaccttcc tgctaacgcc gcatttgcca gcacgctgaa
    16261 aaaccccaaa ctcggctatg ttagccgtta tgcgctgggc cgtgactatc acaaacttct
    16321 gcgcaaccga ctcaaaaagc tgggcgagat gattcagcaa cattgtgttt cgctgaattt
    16381 tagaccgttt gtcgattctg cgcctattct cgagcgcccg ttagctgaaa aagctgggct
    16441 cggctggaca ggtaagcact cacttatcct caatcgcgag gccggttcgt tcttcttttt
    16501 aggcgaattg ctggtcgata ttccgctgcc cgtggatcaa ccagtcgagg aaggatgcgg
    16561 caaatgcgtg gcctgtatga cgatttgccc gaccggtgcc atcgtcgagc catataccgt
    16621 cgatgctcgc cgctgtatct cttatctcac catcgaactt gaaggggcgc tcccggaaga
    16681 gttgcgaccg ttaatgggaa accgtattta cggttgcgat gactgccagc ttatctgccc
    16741 gtggaatcgc tattcgcaac tcactacaga agacgatttc agcccgcgta agccgctaca
    16801 cgcaccggaa ctcattgagt tatttgcctg gagcgaagag aagtttttaa aagtcacgga
    16861 aggatcggcg attcgccgta taggtcacct gcgctggctg cgtaatatcg ccgtggcatt
    16921 aggcaatgcc ccttgggatg aaacgatttt gacagcgctg gaaagtcgta aaggtgagca
    16981 cccacttctc gatgagcaca tagcgtgggc gattgcgcag caaatagaga gacgaaatgc
    17041 gtgcatagtc gaagtgcaat tgccgaaaaa acagcgtctg gttcgggtga ttgaaaaagg
    17101 gttaccgcgt gacgcctgat tcatccacag cttgtgaata aaaataaaaa cgcattgcaa
    17161 ttcaagacga ataaaatcgt caagtgatcg actcgacaaa acggaatctt gatttttaac
    17221 ttatttatca ataggttaaa aaaataccat tcaccgtgcg aagtttcttt gaaattaaga
    17281 ttacagcctg aggctgtgga taactctgtt tacaagagtt tgtcagagac aacaaaaacc
    17341 agcaataacg catcgcgtcg ctgtagatat tttattgaga gaaga
//
LOCUS       DAJTSA010000085        16729 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000085 DAJTSA010000000
VERSION     DAJTSA010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16729
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.085"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..290
                     /locus_tag="OO369_002994"
                     /pseudo
     CDS             1..290
                     /locus_tag="OO369_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            963..2471
                     /gene="ltrA"
                     /locus_tag="OO369_002995"
     CDS             963..2471
                     /gene="ltrA"
                     /locus_tag="OO369_002995"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5983189.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
     gene            2561..3034
                     /locus_tag="OO369_002996"
                     /pseudo
     CDS             2561..3034
                     /locus_tag="OO369_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            3199..7317
                     /gene="pic"
                     /locus_tag="OO369_002997"
     CDS             3199..7317
                     /gene="pic"
                     /locus_tag="OO369_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HCR5983190.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(7468..7710)
                     /locus_tag="OO369_002998"
                     /pseudo
     CDS             complement(7468..7710)
                     /locus_tag="OO369_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(7749..8699)
                     /locus_tag="OO369_002999"
                     /pseudo
     CDS             complement(7749..8699)
                     /locus_tag="OO369_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(8750..9325)
                     /locus_tag="OO369_003000"
                     /pseudo
     CDS             complement(8750..9325)
                     /locus_tag="OO369_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            9329..9729
                     /locus_tag="OO369_003001"
                     /pseudo
     CDS             9329..9729
                     /locus_tag="OO369_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10132..10713)
                     /locus_tag="OO369_003002"
     CDS             complement(10132..10713)
                     /locus_tag="OO369_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983191.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(10760..14617)
                     /gene="sigA"
                     /locus_tag="OO369_003003"
     CDS             complement(10760..14617)
                     /gene="sigA"
                     /locus_tag="OO369_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR5983192.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(14978..15982)
                     /locus_tag="OO369_003004"
                     /pseudo
     CDS             complement(14978..15982)
                     /locus_tag="OO369_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            15967..16344
                     /locus_tag="OO369_003005"
                     /pseudo
     CDS             15967..16344
                     /locus_tag="OO369_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(16383..>16729)
                     /locus_tag="OO369_003006"
     CDS             complement(16383..>16729)
                     /locus_tag="OO369_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5983193.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         4195 a         3959 c         4240 g         4335 t
ORIGIN      
        1 cccgtgcgca gcgcgatgac tggctgaaga aagagataca gcgcgtatac gatgaaaatc
       61 acaaggtata cggtgtgcgt aaagtctggc gtcagttgtt acgggaagga atcagggtgg
      121 ccagatgtac agtggcgcgc ctcatggcgg ttatgggact tgccggtgtt ctccggggta
      181 aaaaggtccg tacgaccatc agccggaaag cctttgccgc aggcgaccgc gtaaaccgtc
      241 agttcgtggc agaacgacct gaccagctgt gggtggctga ttttacttac ggtgtgcccg
      301 gagttcaggg cgggcatgga tgcttaaatg aaccgcgagt ctgtctggaa tattgaaccg
      361 gtaactcacg atgagaaacc caacaatccc accgggtgtg acggtggaga acctgagcgg
      421 cagtgacctg cggcatgccc gcagggtgat gtaacccgct gacaacgggg attgaggcga
      481 gatcactaag ccgagatgat cctcaaggtt aagtactgaa aggctgaaga acatgaaccc
      541 gttaatccgc ctctgtgggt tgaaaacgtc accacggcct acgtgatctg acaggccgtg
      601 caggaggaac tggcagtgat acgtaagcac tgccggtcga aggtgttttg acatgtatgc
      661 gaaacaccgg ggcagcagcg tctatcacac ttgcgttgct gacttctgcc aacttgcggc
      721 aagcaaggat aaagagtgcg acgggcagcc tcctcagtat gcctgagtcc aggcaggtaa
      781 accggggaag gtcagcgacg gatgttaagg gggcatggct ccgatgacgc gctggctggc
      841 ggagcttccg tagtagtccg cgatggggaa agcccattac atggcgaagg gaagcagttt
      901 gaatgtgttt gcgacgtgaa ttaactgacc taacgaggtg aagacctttg ataatcagcg
      961 aaatgcaacg caagcttgcc acatgggcag ccaccgatcc gtccctacgg attcaacggc
     1021 tgctgcgtct gataacacaa ccagaatggc tggctgaagc ggcgcggatc acgctttcat
     1081 caaagggggc ccataccccc ggcgttgatg gcgtgaacaa aacaatgcta caggccagac
     1141 tggctgttga gctgcaaatc ctcagggatg aattactctc aggccactac cagcccttgc
     1201 ccgccagacg ggtttacatc cctaaaagca acggcaaact gcgaccactg ggtatccccg
     1261 cgttgcggga tcgtattgtt cagcgggcca tgctgatggc gatggagccg atatgggaga
     1321 gtgattttca tacgctctca tatggcttcc ggcctgagcg cagtgtccac cacgcgatcc
     1381 gcacggtgaa attacagctc acagactgtg gtgaaacccg gggacgctgg gtgattgaag
     1441 gcgacctgtc cagttacttc gacaccgtac atcatcgact gctgatgaag gctgtacgcc
     1501 gcaggatcag tgacgcacgt ttcatgactc tgctgtggaa aaccatcaag gcgggacata
     1561 tcgatgtcgg tctctttcgg gcggccagtg aaggtgtgcc acagggcggt gttatatcgc
     1621 cgctattatc gaacatcatg ctgaatgagt tcgatcaata cctgcatgag cgctacctga
     1681 gcgggaaagc cagaaaagat cggtggtact ggaataacag tatccaacgg ggccgaagta
     1741 cggcggtcag agaaaactgg cagtggaaac ccgcggtggc gtactgccgc tatgccgatg
     1801 attttgtcct catcgtcaaa ggcaccaaag cacaggcgga agccatcagg gaggagtgtc
     1861 ggggtgtgct cgaaggcagt ctgaaactca ggctgaacat ggataagact aaaatcaccc
     1921 atgttaatga cggctttatc tttctggggc acaggatcat tcgcaaacgc agtcgttatg
     1981 gcgagatgcg agtggtctca acgatcccgc aggagaaagc cagaaacttc gccgcatcgc
     2041 tgacagcact gttatcaggc aactacagtg aaagcaaagt cgatatggct gaacaactca
     2101 accgaaaact gaaaggctgg gccatgttct atcagttcgt tgattttaag gccaaagtct
     2161 tcagttatat cgaccgtgtc gtgttctgga agctggctca ctggctggcc cgcaaatacc
     2221 gtacaggtat cgcttccctg atgaggtggt ggtgtaaatc accgaaaccg ggtcagagca
     2281 aaacgtgggt tttatttggt aaaaccaatc acggcaagct cagcggcgaa atactgtacc
     2341 ggttggtggg gcaaggcaag aagctgttcc gctggcggct acccgaaggt aatccctatc
     2401 tgaggacgga gaccagaaac acgtatacat cgcgctttac agaagtggca atggcgttcg
     2461 ccagcattta aatggagagc cggatgcgct gaaaggtgca cgtccggttc ggggaggaga
     2521 ggcagggaaa tagtccgact acgccctgcc tcttactcta ctcagcacat ggcagggctt
     2581 cgtctatgtg gcgttcatca ttgatgtgtt tgccggatac atcgtgtggt gacgagtctc
     2641 atcgtctatg gaaacgacat tcgtgctgga tgcactggag caggcgttgt gggcccgtcg
     2701 tccgtctggc accatccatc acagcgataa aggctctcag tatgtgtcac tggcctataa
     2761 ggcgcgacta aaagaagcaa aactgctggc atcaacaggg agtactggtg actcgtatga
     2821 caacgcgatg gcagagatca tcaagggcct ttacaaagcg gaggtaatac accgtaagag
     2881 ctggaaaaac cggacagaag tggagctggc cacactcacg tgggtggact ggtataacaa
     2941 tcgacgattg ctggaaaggc tgggccatat cccaccggca gaagcagaaa aagcttatta
     3001 tgcttccatc ggaaatgatg atctggcagc ctgagttcac agataaaaca ctctccagga
     3061 aacccggggc ggttcagttc acaaaaacac attaatgcag taactatatt ttcctttctg
     3121 gtgataacgt cgggttatca ttagcttctt cagctatttt acttttatat cccttgtaaa
     3181 catcatggag aatccatagt gaataaagtt tattctctta aatattgccc cgtcaccggg
     3241 gggcttattg ctgtctctga acttgcccgc agggtaataa aaaagacatg ccgaagatta
     3301 acgcatattc ttctggctgg cattccagca atctgtctgt gttactctca gatatcccag
     3361 gcgggtattg tccgttccga tattgcctat cagatttatc gtgatttcgc cgaaaacaaa
     3421 gggctttttg tacctggtgc caatgatatt ccggtatatg ataaggacgg aaaacttgtg
     3481 ggaagactgg gtaaagcccc aatggccgat ttcagcagtg tgagctcaaa tggcgttgct
     3541 acgcttgtat cgcctcagta tatcgtcagc gtaaagcata acggaggata tcggagtgtg
     3601 agctttggta atgggaaaaa tacatattcc cttgttgacc gtaataacca cccttctatt
     3661 gacttccatg ctccacgtct gaataaactg gttacagaag ttattccctc agcggtaaca
     3721 tcagaaggaa ccaaagccaa tgcttataaa tacactgaac gttacaccgc tttttatcgg
     3781 gtgggtagtg gtacgcagta cactaaggac aaggacggaa atttagttaa ggttgccggt
     3841 ggatatgctt ttaaaacagg aggaaccaca ggagttcctc tgatatctga tgcaacaata
     3901 gtctctaatc ccgggcaaac ttataatcct gtaaacggcc ctttacctga ctatggagcc
     3961 cctggggata gtggttctcc tttgtttgct tatgataaac aacaaaaaaa atgggttatt
     4021 gttgctgtat taagagcata tgcaggtatt aatggtgcta cgaactggtg gaatgtcata
     4081 ccaacagatt atctgaacca ggttatgcag gacgatttcg atgcccccgt agactttgtt
     4141 tccggactgg gccccctgaa ctggacatac gacaaaacat caggcacagg taccctgagc
     4201 cagggcagta aaaactggac catgcacggg cagaaagaca atgacctcaa tgccggtaaa
     4261 aatctggtat tcagcgggca gaatggtgca attatcctga aagacagtgt gactcagggt
     4321 gccggttatc tcgaatttaa agacagttac accgtatctg ctgaatccgg aaaaacatgg
     4381 acgggtgccg gcattattac tgacaagggg acgaatgtaa cctggaaggt caacggcgtt
     4441 gccggtgaca acttgcataa gctgggggaa ggaaccctga ccataaacgg aacaggtgta
     4501 aacccgggag gactgaaaac gggagacggt atcgttgtac ttaaccagca ggcagacact
     4561 gcaggtaata tccaggcctt cagttcagtg aacctcgcca gcggacgtcc gaccgtggtg
     4621 ctcggggatg cccgtcaggt caatccggat aacatttcat ggggataccg gggaggtaag
     4681 cttgacctta atggtaatgc cgttaccttc acccgactgc aggctgctga ttacggggcg
     4741 gtgattacaa ataatgcaca gcaaaaatcc cagcttttac tggatcttaa ggctcaggat
     4801 acaaatgtca gtgaaccgac gattggaaat atatccccct ttggtggtac cggcactcca
     4861 ggaaacctgt acagcatgat actcaacagc cagacccgct tctatattct gaaatctgcc
     4921 agctatggta acactctgtg ggggaacagc ctgaatgatc cggctcagtg ggagtttgtt
     4981 ggcatgaaca aaaacaaagc agttcagaca gtaaaagata ggatcctggc cgggcgggca
     5041 aaacaacccg ttatctttca tggtcagctg accgggaata tggatgtcgc cattccacag
     5101 gtgccggggg gaagaaaggt aatctttgat ggtagcgtga acctgccgga aggtaccctg
     5161 agtcaggaca gtggcaccct gatattccag ggacatccgg ttatccatgc ctccatcagt
     5221 ggcagtgcac cggtcagcct gaaccagaaa gactgggaaa accgtcagtt tacaatgaaa
     5281 acactgtcgc tgaaagacgc tgacttccat ctttcacgta acgcctcgct gaacagtgac
     5341 attaagtcgg ataacagcca tatcacactg ggaagtgaca gggcatttgt ggataaaaat
     5401 gacggaacag gaaattatgt cattccggag gaaggtacct ctgtcccgga caccgtgaat
     5461 gacaggagcc agtatgaagg gaatattacg ctgaaccata actcagccct ggatatcggc
     5521 agcaggttca ccggggggat tgacgcttat gacagtgccg tcagcatcac ctctccggac
     5581 gtcctgttga cagccccggg tgcttttgcc ggcagttcac tgacagtgca tgatggcggt
     5641 catcttacag cactgaacgg tcttttcagc gacgggcata ttcaggccgg taagaacggc
     5701 aaaatcaccc tgagcggtac accggttaaa gatacggcta atcagtatgc ccctgctgta
     5761 tatctgacgg acggatatga cctgaccggc gataacgcag cactggaaat tacccgtgga
     5821 gcacatgctt ccggtgatat tcatgcctct gcggcatcaa cagttaccat cgggtctgac
     5881 acgccggcag aactggcttc tgcggaaacg gctgcatcgg cgtttgccgg cagtcttctt
     5941 gagggctata acgcagcatt caatggtgcc ataaccggtg gcagggctga tgtcagtatg
     6001 cataatgcac tgtggactct gggtggggac tctgccatcc acagtcttac cgtcagaaac
     6061 agccgtatta gttctgaagg agaccgtaca ttccgtaccc tgacggtgaa taaactggat
     6121 gcaacaggca gtgattttgt tttgcgtacg gacctgaaaa atgccgataa aattaatgtg
     6181 actgaaaaag ccactggttc agataacagc ctgaacgtca gctttatgaa taatcctgct
     6241 cagggacagg ccctgaatat tcctctggtc acggcaccgg cgggaacttc agcagagatg
     6301 tttaaggccg gcacccgggt gacaggtttc agtcgggtga ccccaaccct gcatgttgat
     6361 accagtggtg gcaatacgaa gtggatactg gatggtttta aagcggaggc tgataaagcc
     6421 gctgccgcga aggctgacag tttcatgaat gccgggtata aaaacttcat gacggaagtt
     6481 aacaatctga acaaacgtat gggtgacctg cgtgacacaa acggtgatgc cggtgcctgg
     6541 gcgcgcatca tgagtggtgc cggttctgca gacggtggtt acagtgataa ttacacccat
     6601 gttcaggtcg gctttgacaa aaaacatgaa ctggacggtg tggacctgtt taccggtgtc
     6661 acgatgacct ataccgacag cagtgcagac agccatgcat tcagcggaaa gacgaaatcg
     6721 gtggggggcg gtctgtatgc ttcagcattg tttgagtccg gtgcctatat cgatttgatt
     6781 ggtaaatata ttcaccatga caatgattac acaggtaact ttgctagcct gggaacgaaa
     6841 cactacaaca cccattcctg gtatgccggt gctgaaacgg gttaccgcta tcacctgaca
     6901 gaggacacgt tcattgagcc gcaggctgaa ctggtttacg gcgccgtgtc cgggaaaaca
     6961 ttccgctgga aagacggtga tatggacctg agcatgaaga acagggactt cagtccgctg
     7021 gttggaagaa caggggttga actgggcaag accttcagtg gtaaggactg gagtgtgacg
     7081 gcccgtgccg gaaccagctg gcagtttgac ctgctgaata atggagagac cgtactgcgt
     7141 gatgcgtccg gggagaaacg gataaaagga gagaaggaca gccggatgct gtttaatgtt
     7201 ggtatgaatg cgcagataaa ggacaatatg cgctttggtc tggagtttga gaagtcagcc
     7261 tttggtaaat ataacgtgga taatgcggta aacgcgaatt tccggtatat gttctgatgt
     7321 gggggtaaag ggctgccgga aggcagcctt tctcagagac tcttaaaata taaaactgaa
     7381 ggtcttcggt aagggggagt gtattagacg tcaactagtt ttgccgactg gcgcgagcct
     7441 gtttaagatt ctgtgtaaat gcctttctca gaagtgaccg tccaggcggt caccgaactc
     7501 gataataaag cggctcattg ccattcgcca gtccttcaac ggcatcgtcc atttctggga
     7561 cgcagactgg attgccagcc acaccacttt tttcaccgag tcgtctgtcg ggaacacttt
     7621 acgctttttg atcgcatggc ggatcacgct gtttagcgac tcgatggcat tcgtcgtata
     7681 gatcactttg cggatgtccg ttggataagc ggtaagcgta cagcgagggc cgtattgacg
     7741 gggatgtgtt attcagctgg cagtgctatg cgccacggaa gcagttcgct gacccggttg
     7801 accggccagt ctgctatgac gccaagcaca tggcgaaggt agctttctgg atccacgtca
     7861 ttcagtttgc acgtcccgat caggctgtac agtagcgcgc cccgctcacc accatggtca
     7921 gagccgaaga acaggaagtt tttacgaccc agactgaccg cccgcagggc attttcagcg
     7981 atgttgttgt cgatttccac ccagccatta ctgcagtaca cgttcagggc cttccactgt
     8041 ttcagtaggt atgcgaacgc ttttgccgta tccgagtgac gcgacagtgt tttcatctgt
     8101 tgctgtatcc ggtcatacag tgactgcatc agtggcgcgg ctcttgcttt tcttgccgcc
     8161 agacgctgtt ctgctgaaca tccccggact tctgcctcga tggtatacag ttcaccgata
     8221 cgctgcaggg cttccgtggt gatgtcggtt ggcactcttg catgcacatc gtggattttt
     8281 ctccgggcat gggccataca cgcagcttcc gttattctgc cggattcgta taacgcccgg
     8341 taaccaccgt aagcatctgc ctgaagcaca ccgctgtaac cggctaggtg attctgtgga
     8401 tggatacctt tccggtccgg gctgtacgcg aaccagaccg ccgggggcat ctgtgaaccg
     8461 gcgttacggt catcacggac atagacccac agccgggcag ttcttgtttt accactgccc
     8521 ggctcctgga ccggaacggg gatatcatcc gcatgaactt taccgggcat cagtacatac
     8581 tggcgcagga cgtcatacag cggctccagc agttcagcaa cggcaccggt ccagcgaccc
     8641 agtgtggcac ggctcagctc cacgccctga cgacggtata tttctgactg gcggtataac
     8701 tgtcaacgac ggatgaaaag tggctcttcc gcaacctggg tggagatttt catgccagtg
     8761 gactcattac cctctgcctg agtagttcaa atcctgctcg accatacatc tggcgtttca
     8821 gcatcttctt caggcgattt acatgacctt cgacaacacc attactccag cgactactga
     8881 ttgcctcaca tatagctgca gcatcagctt ccatccccgc cgccacgcgc cgaaattctg
     8941 ctgaaccgct ttcgtggact cgagtgaacc aactgcttag ctgtgattta ttttgggttt
     9001 tcagaatacg gtagaactcg agtgccagtt gctgcgctat tttcagctca ggttcttttt
     9061 cacacatcag actaataaat cgggaagcat aattttcttc atccctggtt attctccagg
     9121 gcatcaacca gcggcttacc cgggagactg aaggaagtct gacggcagta gtaaccggtg
     9181 ggttccagcc tttacgccat ttggcaaccg catccctgac gatggtttca ctgcctgtaa
     9241 atccccgggc caccatttcc cgccatatcc ggctggcgtt ataattacca ctttctcgtt
     9301 gctcttttaa ccactccctc catggtgtca gcgaaaaacg aaaccgctgt tgaaatccct
     9361 ggaaagctgg ttgcggaaaa gatgaagacc cagtcgcgac actcagagtt ggcgaaggcg
     9421 ttcgcgtacg cacttaacca gtggccggca ctgacgtact atgcgaacga tggctgggtg
     9481 gaaatcgaca acaacatcgc tgaaaatgcc ttgcgggcgg tcagtctggg tcgtaaaaac
     9541 ttcctgttct tcggctctga ccatggtggt gagcggggag cgctactgta cagcctgatc
     9601 gggacgtgca aactgaatga cgtggatcca gaaagctacc ttcgccatgt gcttggcgtc
     9661 atagcagact ggccggtcaa ccgggtcagc gaactgcttc cgtggcgcat agcactgcca
     9721 gctgaataac acatccccgt caatacggcc ctcgctgtac gcttactcat cacctgctat
     9781 taatccccga gtcagtactt cgcggcctga cgcgatcacc ctactcagta cttcagagcc
     9841 cgcctccggt acctgcctgg cgatcgcccc tgtgcaacaa aataaatcaa ccgttatcaa
     9901 accacgatcg ttcgtatgcg ccgccgatga cgcgcgcctg aaaaacgcgt caatatgagt
     9961 tccccggaag cttacgataa tacgcttatg aacattacaa cacgctcatg cggaacctgg
    10021 atgggggaag gaacggtgaa gctgttagcg aatgaccggc gtttatacaa acgccggtca
    10081 tgatgaatga tatttcagaa tatacctcgg gctaaaatac cgggacagag atcaggcgtg
    10141 attaatctgc agcattttta ataacagaag tactgagctt actttacctt tgatgatgta
    10201 atagttattt ccgtcggtac tcatttttcc ctgaaataat gtcacggatg tttcttcctg
    10261 ctgatcctgc aggcgcagtc gtaaggtcat atttttaatg agctgagatc cgggtggcaa
    10321 ggggtatttg ctgtcagata ctgggcgact gagtctgatg tggcgcgtat acagcccgtt
    10381 ttcgtacagg gtggcctctg ttatggtgtc agtgaccagc atgtgggaga caatgctgta
    10441 ttctttttta aagcacccac cagaaacggt gattgtaatg ataatggtct tctcgatcga
    10501 gatgtaaata cccgtttcgt tatactgtcc gtatcggaac catgagaact cagttgcatt
    10561 ccagccatgc tccagaatat gtgcattttt ttgcctgata gtttcttttt ttattcgctc
    10621 atgtttaagg cataacaggc agataaataa tgtcagaaaa agaaagctaa taatgagagc
    10681 aagagcgata tagtctgtca cagggtaata catattctct ccggtagaag aagggccgca
    10741 aacgcggccc gggctgttat cagaaagagt aacggaagtt ggcgttgatc gcgttatcca
    10801 cgttgtattt accaaatgcc gatttctcaa actcaagacc gaagcgaaga ttatcgcgaa
    10861 tttcggcgtt gagaccaaca ttcatgagaa tacgaccgtc tttttcacct ttgatacgtt
    10921 tctcaccgga cgcatcacgc agtacggttt caccgttggc aaacaggtca aactggtagc
    10981 caaggccggc gcgggctgtg actttccagt ccttaccgga gaaggattta cccacatcaa
    11041 caccggtacg cccaatcagc ggattaaagt ccttatcctt catggtcacg ctcattccgc
    11101 gatcctgcca gtcgaaccgt ttacctgata cggccccata aaccagttct gcctgcggct
    11161 caatccagga gtcttctgtt acatgatagc ggtagcctgc ttcagcaccc acatacaagg
    11221 aatgagaact gtagtctttt gttccgagtc cagcaaaggt cgccgagtac tcattatcat
    11281 ggtgaacata cttactaatc aggtcgatat aggcaccaga gtcaaatata gcggaagcat
    11341 acagacctgc cccgacggac ttcgtcttgc cactgaatgc attactgctg gcatgactgt
    11401 cggtatacgt catagtcaga ccagtgaaaa ggtcaagtcc atccagctca tgttttttat
    11461 ccacaccaat ctgcacatgg gtgtagttgt cactgtatcc accagaagct gaacctgctc
    11521 cgctcatgat gcgggcccat gcaccggcct cgccattggt gtcacgcaga tcccccattc
    11581 gtttattcag gttgttgact tctgtcagga aggctttgta attaccggac atatacgatt
    11641 ttgctgcacc ggaagatgct gtattttcag ccaccttata tccggtcagg gtccacgtgg
    11701 tatgttcctg atcctctttc gtgctgatca caggagatat gttgctgaaa cctatggcct
    11761 gagtactggc tttaaatatc tctttattgc ttccttttgg ggctgttatc aggtcaacgt
    11821 ttaatcctga gttattattt cccactttct gtatatcgac cgacagactg ttattgcttc
    11881 cggttaactg atttttaaca atgagtttat ccgattgatt tacagactgg ctcagatcag
    11941 cccgcataat cacagcagta ttagtggcat ccagtgtgtt tactgtcaca gtatggaaac
    12001 ccgaattctc tatgttacta aacaacaaaa gactgtcgcg ggtttttaat gattgcaggg
    12061 ctgactgtcc gtttactctc cataaggtgt tatacatgct ggctgaggct gcaggagcat
    12121 caatacctcc ggtataagaa gtatcaaagc cacccagcaa acctgtagcc agcggtgtgt
    12181 gtgtggtttt gtccaggtta tcttctttat actggtcaac accaaacaga atagatgctt
    12241 ttttgtctga ttttatatta ccattgaccc aggcataatt ttgagccttt aacgtggcac
    12301 catcttctgt catattatat ccatctgaca tatagattgc cgggctgtat ttattatttt
    12361 ctgtaggggt gcctgttaat gtcagggttc cggccagttc aatagcttta tctacataca
    12421 tttttccctg ggcggtaaca gtagctcctt tgttgataat caggtctgta ttaattaagg
    12481 ataatgcacc agtcatagta acagttgatt tatcaccaac agttattttg ctgttttgag
    12541 cactcagact gccgttgaaa ctactttcgc ctgctgaatc cccggagcga agctgttgtc
    12601 tgaatccaaa accttcgcca gtaatgtttc ttcctgagaa catgtcaatg tatgcagttt
    12661 tatttcccag aacaatttga gagtttttag cctctatgtg tccttttaca ttagcatccc
    12721 ggcctatact cagggttgcg ttttctaaat ttaatttccg gaaattaaat ttacgacttt
    12781 cccagtcggg ctgggaaaaa gaggctatct gattgttgga cttatattcc gtattgtaac
    12841 gtttatttac agtgctttcc tggtccttta tggcagcaga atagtctttt tgacataaaa
    12901 aaggaattgg acagttgtta ttgccttctt taaatatggc atgttctgtc gcatgtcctt
    12961 gtaatgttaa ctgtgcattc cggacagaga tatcgttttt gatatcgact gagccatcaa
    13021 agatcagatt ggcattattg ttatgttgct gggtagtatt gataatatga tttatgctta
    13081 tattacctga tacattccca tggtacatat atgcatcagt attagtaagt gataatgttg
    13141 attgcttcac gtttgagttc gttacggttg ttcccgcatc tgttgctgcg attttctgaa
    13201 atgactggtc atagccgttt agatccagtg ttccgccatt tctggtaaaa tagatttcgc
    13261 cattaccgct ttcgcttaaa gcgtctttgg catttatttt taccgtacct ttacctccgg
    13321 ccatgtaaat tttattgaag gctttttcag cactaaggat gacagtccca ttacctattt
    13381 taaggttatt tccctgtgct atttttacat ccagagtacc actacctatt ttatgcagat
    13441 tatctccggc aacgccctta acattccatt caacggtggt attattatca atatctatcc
    13501 cggcaccttt ataagactta tcttttccgt acactttata tgtctggttc ttatcgaata
    13561 taagcccgcc aatgccaaga tcggtatcct gcttgagcgt taatacccct cctcctgaga
    13621 cagacaggtc cttatctttt ataatcgatg ataactcttg ttttttacca tccagctgca
    13681 tagtgtttgt gaaattcttt tttaaattgt ttaccagaat attattaaat aacgagtagt
    13741 ttgtccaggc tatattggta ctactgataa aaccaactgt actggtaaca ccatggaaaa
    13801 cccatttttg ttgttcttta tcatatagat aagaaccaga accactatcg ccaaaaattg
    13861 gtgagttttt aaaaggggtt ctgttggtat aaatgtaact cttgtttttc cagtccagat
    13921 taaaaagact tgcgcttaac aagtcgggat tatagttttg tccaaacaga tattgtttgc
    13981 cgtttaatat cgttgaggtt gttccggcac ctgctctgaa gccaattatt tgttccttac
    14041 cgttgtagtt tacgccatat cttttcatcg catcttcggg agaaagtgag aagtcggcac
    14101 tctccgtaac ccctgttgtt tccacgacga atttattcag acgatgaacc gaaaagtcgc
    14161 cagatgatac tctgttactt gctttatatg ctgtctgatc ccatgactgt gttgttattg
    14221 catgatgctg tgtaccgtta tgagtcgcag taacactgta cgcacctcca attgatgttg
    14281 ttgcaccttt gttggaaaca gcagaaaaat caggtacagg tagttctgga aaatgaaata
    14341 tctttccatc tttcatcatt aattgaacat ttgttgcacc cggtctgaac tctcctctgt
    14401 tttgagcaag gtcaagataa tcgcgggtcc atatattatt tatatccagc attgctgcaa
    14461 atgctgtttc gccataactt ccatatattg aggataaaat aataccgagg ataactttct
    14521 ttcttgatgt accgacacta actttccggg tcagttcaga aacagcaact aatccacctg
    14581 taatatgact atatttcagt gaataaattt tattcatacg tgaactccga tatttctgag
    14641 ttgtggttat gggatagcat ttaattacaa cggggaacta tcagtcatgg tacgtaactg
    14701 ataagggtat aatgataatc actgtcagtt atatttgtat gacagatgta acaaaatgaa
    14761 aaaaaagata agcacaagtg attgattggt tagatattga tcacttggtg tttgtatttt
    14821 gaattttgcg atcaaaattt gatctttgtt gtttccggtc aatcctttac aggttttctg
    14881 tattatccgg gggagttaag tctgtcgatg tccgacagac ttttgatgat ggaaacaaca
    14941 tgcagcgata atgtgcatgc tgttttgcag gtggaactca gaaggtgaac tggaactgtg
    15001 cgaccgcacc gtaagtgttg tccgtgccga tcaggcctgc aacatcaagg tgaaccacat
    15061 caaacggtga gatccccacc ccggccgtga atgcactgcc gtcgttaccg gccagattct
    15121 gacggtaacc ggcgcgcagc tgtgcccatt tccaggcatt aaattctgtg ccaatggcgg
    15181 caaactgacg gttgctgtcg gaggtgaaac cgctggccgg ggtcagatcc acatcaaatg
    15241 cagtggtgaa catcgcgttg tgccaggaga caccggccgt cacctgcggg cggatcctga
    15301 aggtttccgt gataccgttc acttcttttg tctcaatact acgggagatc aggttctgtg
    15361 cgaccagtcc cagcgtccag ttgtcatcca ggtccgtact ggcaccgatg tcggcgttga
    15421 tgccattttt cgtgttgtgg tagcggtcgc ctttgaaggc gctgctgtca taatttctga
    15481 ccagcacgtt gtagttgaac aggtcgacgc gctggaattt gggggggaag cccagagacc
    15541 atttctgccc ggccgtttcc agttccgtag ccagagcaat acccacatca gtgatcagtg
    15601 ccgtgcgggc acaggcctgt gatgtcaggc ggcttttgtc cacctcctgc ccggtgctgt
    15661 ttgccacctc ttccaggtaa gtgaggtcag catcagagac tttaccgtcg acactgacgg
    15721 tgccgtgtga tttaaccatc agtgcggcag agaggcgatc accgggaagg gccgccacag
    15781 cagaaacgcc cagctgtgcg gcggcatgtg tgttacggaa gtcccggagg tgttctttca
    15841 ggcgtgcggc agcctcagga acaccgtggt tactttcgat ggcgcggtcg aaggccttcc
    15901 actcatcact gatccggtcg gcgttgtcgg tgatattatc cggattggag agctgcgcgc
    15961 cgacggacgg aagcaggatg ctgggctcat gcgcgacgca aaatccacga tgtatatatc
    16021 agtactaaaa gcgcgacggc ggaagaagct ctgaaactaa tcggtgaact gtacgccata
    16081 gagcacgata tacgcgggtt accggtgtct gaacgcctgg cggtcaggca aatgcagagt
    16141 agaccgctcc tgacttccct gtataagctg atgcaggaga aagaacacac gttatcgaag
    16201 aaatgtcgtc tgggagatgc gttccggtat atcaggaagc actgggctgc gttgtgcaac
    16261 ttctgtgatg atggcctggc tgaggtagat aataatgcag cagaaagagc gcttcgtgca
    16321 gtctgtctcg ggaagaaaac tttttcgagg atcactcatt ccgtgtctga ctgcttcagg
    16381 gtttaatttt gcttctgctg actgaatgat gattgggcag caagtgcaat atcccacata
    16441 aatccagtca actcacgagc caccgccgta atggctactt gtgatttttt cccttttgct
    16501 gtcagagttc ggtaacgaga acacaatcgt tgttgagctt tccatgcaat atctttggaa
    16561 tattgtgtta cagagtccgg tcggtaatat ataagccatg ctccaacttt tgcaggtgta
    16621 cgataagacc aggcggcttc gtaaagcaga cgtctcagtt cgctgtttcc aacttttgtt
    16681 attcctctgg gacgaatact gtttccactg gaatattcac ctggcacca
//
LOCUS       DAJTSA010000086        16426 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000086 DAJTSA010000000
VERSION     DAJTSA010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.086"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..73)
                     /locus_tag="OO369_003007"
     CDS             complement(<1..73)
                     /locus_tag="OO369_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HCR5983194.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQL"
     gene            complement(171..1280)
                     /gene="anmK"
                     /locus_tag="OO369_003008"
     CDS             complement(171..1280)
                     /gene="anmK"
                     /locus_tag="OO369_003008"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR5983195.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1554..2021
                     /gene="slyB"
                     /locus_tag="OO369_003009"
     CDS             1554..2021
                     /gene="slyB"
                     /locus_tag="OO369_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR5983196.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="OO369_003010"
     CDS             complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="OO369_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR5983197.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2703..2939
                     /locus_tag="OO369_003011"
     CDS             2703..2939
                     /locus_tag="OO369_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR5983198.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2942..3799
                     /locus_tag="OO369_003012"
     CDS             2942..3799
                     /locus_tag="OO369_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5983199.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3799..5811
                     /locus_tag="OO369_003013"
                     /pseudo
     CDS             3799..5811
                     /locus_tag="OO369_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5812..6333)
                     /gene="sodC"
                     /locus_tag="OO369_003014"
     CDS             complement(5812..6333)
                     /gene="sodC"
                     /locus_tag="OO369_003014"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR5983200.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6414..7310)
                     /locus_tag="OO369_003015"
     CDS             complement(6414..7310)
                     /locus_tag="OO369_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR5983201.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7359..7598)
                     /locus_tag="OO369_003016"
     CDS             complement(7359..7598)
                     /locus_tag="OO369_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR5983202.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7701..8300
                     /gene="nemR"
                     /locus_tag="OO369_003017"
     CDS             7701..8300
                     /gene="nemR"
                     /locus_tag="OO369_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR5983203.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8337..9434
                     /gene="nemA"
                     /locus_tag="OO369_003018"
     CDS             8337..9434
                     /gene="nemA"
                     /locus_tag="OO369_003018"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR5983204.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9515..9922
                     /gene="gloA"
                     /locus_tag="OO369_003019"
     CDS             9515..9922
                     /gene="gloA"
                     /locus_tag="OO369_003019"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR5983205.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10025..10672
                     /gene="rnt"
                     /locus_tag="OO369_003020"
     CDS             10025..10672
                     /gene="rnt"
                     /locus_tag="OO369_003020"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR5983206.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10765..12879
                     /locus_tag="OO369_003021"
     CDS             10765..12879
                     /locus_tag="OO369_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5983207.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGL
                     SATVRSASDVAAFLGGDRPVTVVNPPTMRHPQIRIVVPVANMDDVSSVASGTGEDSHA
                     GREGSIWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVD
                     AAHFESTSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLEL
                     GIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVEC
                     MFAGRLENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRTAPWKDLPRRVFDAT
                     LDMLSGRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPE
                     GEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSAQLPF
                     WRGEGNGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATG
                     IVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASD
                     DSIVARIPDTDGKLPDAAIFLF"
     gene            12889..14181
                     /locus_tag="OO369_003022"
     CDS             12889..14181
                     /locus_tag="OO369_003022"
                     /inference="COORDINATES: protein motif:HMM:NF020083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983208.1"
                     /translation="MLQIVREAVGSSALFAARFRECAARALLMPGRTPGHRTPLWQQR
                     LRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQISDVTTTTP
                     SPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELLDPQVIRQ
                     VEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLENLLAVKR
                     IFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLRYLRAHAL
                     VTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRSLQAAREA
                     TRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQLAGVGLPA
                     SLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKAG"
     gene            15131..15223
                     /locus_tag="OO369_003023"
     CDS             15131..15223
                     /locus_tag="OO369_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR5983209.1"
                     /translation="MNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(15267..>16426)
                     /locus_tag="OO369_003024"
     CDS             complement(15267..>16426)
                     /locus_tag="OO369_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5983210.1"
                     /translation="EREMRRFAGACRFVFNRALALQNENHEAGNKYIPYGKMASWLVE
                     WKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPRFKKRGQNAAFRYPQDVK
                     LDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWYISIQTESEVSTPVHPSA
                     SMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKRKI
                     QRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKS
                     GLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKF
                     RCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         3748 a         4344 c         4301 g         4033 t
ORIGIN      
        1 ccagttgatt aaaggcgcta caaccggaga gaaggaccgg caaaatgata attaacagtt
       61 ttttcatagt catatcccga agactttcct ggtccggagg gcaaataccc tccttaatct
      121 tccaagtgta acggcagaca cgataagaaa aaattcagtt aattctgata tcacgagttg
      181 gcggggaaaa tagcccccag taccgtctcc tggcttgcgc cagtgacgga aggcagattt
      241 cctggtaatc ccgccagcgt ccgccaggca agccaggcga aagccaatgc ttccatgtca
      301 tcgccactaa tgccaacggc atcggtggtg gtgacttctg tgcctagcag taataccgcc
      361 agacgcgcca tgagtagcgg attacgacta cctccaccac ataccatcaa tcgttcgcag
      421 ccaccgctca acaaaacttg ttcagaaatg gtcacggcgg tgagttctgc cagtgtggcc
      481 tgaacatcac ggggatcaac acccggaaaa tggcgtaaat ggcgctccag ccagccgtag
      541 ttaaagtatt cgcgtccggt gcttttcggt gcaggttgcg agaaatacgg gtcgctgagc
      601 atattttgca gcagtgggag aataacttta cccgcccgtg cccactcggc atctttatcg
      661 taaggtttac ctgcctgacg ccagatccag gcatccatca gcatgttacc aggaccggta
      721 tcgtagccgc caaccagctg cccaggaatg agcagtgaca gattggcgat gccgccaata
      781 ttgagcacca ttcgtcgctc ggttgggtga gccagcaagg catgatggaa cgcaggtacc
      841 agcggtgcgc cttgtcctcc caaggcaata tcgcgacggc gaaaatcacc gacaaccgta
      901 attccggtgc gtgccacaat ttgattgtta tcgccaatct gtaaagtgtg tggtgccacg
      961 cccgtcggtt catgccagac ggtttgaccg tgacagccga tcgcaactat atctctcgcc
     1021 tgcaggtttt gttccttaag caaggcattg accgcatcag caaaaagtcg cccgagttga
     1081 gtatcaagct gtccaaactg cgaaagtgta agctgctggc cctggcaaat atccagtaca
     1141 gcctgtttca gagacaccgg gatcggccag ctcaagcttg ccagctgtgc gacccggtgt
     1201 tcatcaattg tcgccaacac aacatcaaca ccatcaaggc tggtgcctga cataacgcca
     1261 ataaagcggc ccgatttcat agttcatcct ttttcaatct gacgtttgcg caccactcaa
     1321 acataaactt ttcgtgaata ccatgcggaa tgaccgattt ttaccgttgg tagtaaaaca
     1381 ttatcttcaa atcaataatc atcatgaatg ttttgtttat aattggttga tcctactttc
     1441 attatgattt gctcatattt ggtagaacat gtaaccatgg attcacatat gccatatact
     1501 ttgaccatga gggatgcttg cgtggcgttt catggtgaac aggagatttt tcaatgatta
     1561 aacgcgtatt ggttgtttca atggtaggtc tgtctcttgt cggttgtgtt aataacgaca
     1621 ccctgtcagg ggatgtttat accgcttctg aagcgaaaca agtacagaat gtcagctatg
     1681 gcaccatcgt taacgtacgt ccggtacaga ttcagggcgg tgatgattcc aacgttatcg
     1741 gtgcaattgg cggtgctgtt cttggtggtt tcctggggaa tactgttggt ggcggaaccg
     1801 ggcgttctct ggctactgca gcaggcgctg ttgcaggtgg cgtagctggt cagggcgtac
     1861 agagtgcaat gaacaaaacg cagggtgtcg agctggaaat tcgtaaagac gatggtaata
     1921 ccatcatggt ggtacagaaa caaggcaaca ctcgtttctc tccgggccaa cgtgtcgtac
     1981 tggccagcaa tggcagtcag gtgaccgttt ctccgcgcta aataagtttg cgtgtggtca
     2041 ggttactgac cacacgcccc cttcatttca ccctttggcc tgtaactcaa tgatattatg
     2101 ctcaagtttt gcgatgagcg taatcagttg ctccagttcc tctgcggaga tgccatgtaa
     2161 tatttccgcg cgggttttgt taataacagc ttccatttcg ctgatcagtg gctctgcctt
     2221 ttccgtcagt ttaatacgtt tagcccgacg atcgctggca caagtttgac gcgaaattaa
     2281 ccctttttct tccagttggt ccagagtacg gaccagtgat ggctgctcga tgccaatcgc
     2341 ttttgccagt tgaatttgcg actggtctgg aggtaactga tggatattgt gtaacgtaac
     2401 ccaatgggtt tgtgttaact ccagcggttt caggcgatgg tctatcagag cacgccatat
     2461 gcgcaccaac cgtgccagat cagaacctag tggcgattcc aatttcatct ccttataatt
     2521 agcttgctaa gatattatgc ggcttttaga atagtgtgca gcaattgtat tgctaaaaca
     2581 aatgtattgc tgcatttggt taccgtcaga catatttttc agaaattgcg cgtaaatttt
     2641 tcgcacttaa agaatattta ttaatctaac gcaatatatt cggtcgtaaa ggaatctact
     2701 ttgtgaagtt tatgctcaat gcaacaggat tgcccttaca agatctggtg ttcggtgcgt
     2761 ccgtctattt tcctccgttt ttcaaagcat tcgcgtttgg attcgtcatc tggcttgtcg
     2821 tacaccgcct gcttcgtggc tggatctacg ccggcgatat ctggcatccc ttgttaatgg
     2881 atttatcgct gtttgcgatt tgcgtttgcc ttgctctggc aatactgatt gcgtggtaac
     2941 tatgtcaatt aaaacaatta agtatttctc aacaatcatt gtagcgatag ttgcggttct
     3001 tgccggatgg tggctgtgga attattacat gcaatctccg tggacacgcg acgggaaaat
     3061 acgcgcagaa caggtttcta tcaccccaca ggtgtcggga cgtattgttg agctgaatat
     3121 aaaagacaac caactggtta acgcggggga acttctgctc acgatcgata aaacgccctt
     3181 tcagatcgcc gagctgaacg cacaggctca gctggcgaaa gcgcaatctg acctcgcaaa
     3241 agccaataac gaagcgaatc gtcgtcgtca tctctcacaa aattttattt ttgccgaaga
     3301 actcgatacc gctaacctca atgttaaagc gatgcaggcc agtgttaatg ccgcacaggc
     3361 gacgctgaaa cagacacaat ggcaactggc gcaaacggaa attcgcgccc cggtgagtgg
     3421 atgggtgact aacctcacta cccgcatcgg cgattacgct gacaccggga aaccgctgtt
     3481 tgcccttgtc gacagccact cgttttacgt cattggttat tttgaagaaa ccaagttgcg
     3541 ccatatccgc gaaggtgcac cagcacaaat taccctctat agtgataaca aaacgttaca
     3601 gggtcacgtt tccagtatcg gtcgggcgat ttatgatcag agcgttgaaa gtgactcaag
     3661 ccttattccg gatgtgaaac ctaacgtccc ctgggttcgt ctcgcccaac tcgttcccgt
     3721 ccgctttgcg ctggataaag ttccaggcga tgtcacgctg gtgtcgggca ccacctgtag
     3781 cattgccgta ggtcaataat gaacgcatcg tcatggccct tgcgcaattt gccctggttc
     3841 agggccacgc tggcgcaatg gcgttatgcg ttacgcaata ccattgccat gtgtctggcg
     3901 ctgacggttg cctattattt gaatctggat gaaccctatt gggcgatgac ctcggctgca
     3961 gtggttagct ttcccaccgt tggcggtgtc atcagcaaaa gcctcggacg catggctggc
     4021 agtttactgg gagccattgc ggcactgctt cttgccggac atacgctcaa tgagccgtgg
     4081 ttttttctat tgagcatgtc ggcgtggctt ggcttttgta cctaggcctg tgcgcacttc
     4141 acgaataacg tcgcgtatgc atttcaactg gcgggctaca cggctgccat catcgccttt
     4201 ccgatggtta atattactga ggccagccag ctgtgggata tcgctcaggc gcgcgtttgc
     4261 gaggtgatag tcggtatttt gtgcggcggc atgatgatga tgatcctgcc tagcagttcc
     4321 gatgctactg cccttttaac tgcattgaaa aacatgcacg cccgattact ggaacatgcc
     4381 agtttactct ggcagcctga aacaaacgat gccattcgtg cagcacatga gggggtgatt
     4441 gggcagatac tgaccatgaa tttgctgcgt atccaggctt tctggagcca ctatcgtttt
     4501 cgccagcaaa acgcgcgcct taatgcgcta ctccaccagc aattacgtat gaccagtgtc
     4561 atctccagcc tgcgacgtat gttgctcaac tggccctcac cgccaggtgc cacgcgagaa
     4621 attctcgaac aattgctgac ggcgctcgcc agttcgcaaa cagatgctta caccgtcgca
     4681 cgtattatcg ccccgctacg cccgaccaac gtcgccgact accggcacgt cgccttctgg
     4741 cagcgactac gttatttttg ccgcctttat ctgcaaagta gtcaggaatt acatcgtctg
     4801 caaagcggtg tagatgatca tgccagactc gcacggacat ccgctctggc tcgtcatacc
     4861 gacaacgccg aagctatgtg gagcgggctg cgtacatttt gtacgttgat gatgattggc
     4921 gcatggagta ttgcatcgca atgggatgcc ggtgccaatg cattaacgct ggcagcaatt
     4981 agctgcgtac tctactccgc cgtcgcagca ccgtttaagt cgttgtcact gctgatgcgc
     5041 acgctggtgt tactttcgct attcagcttt gtggtcaaat ttggcctgat ggtccagatt
     5101 agcgatctgt ggcaattttt actgtttctc tttccactgc tggcgacaat gcagcttctt
     5161 aaattgcaga tgcccaaatt tgccgcactg tgggggcaac tgattgtttt tatgggttcg
     5221 tttatcgctg tcactaatcc cccggtgtat gattttgctg attttcttaa ctataatctg
     5281 gcaaaaatcg ttggcgttgc gctggcttgg ctagcgttcg ccattctgcg tccaggatcg
     5341 gatgctcgta aaagccgccg ccatattcgc gcgctgcgcc gggattttgt cgatcagcta
     5401 agccgccatc caacactgag tgaaagcgaa tttgaatcgc tcacttatca tcacgtcagt
     5461 cagttgagta acagccagga tgcgctggct cgccgttggt tattacgctg gggtgtagtg
     5521 ctgctgaact gttctcatgt tgtctggcaa ttgcgcgact gggaatcgcg ttccgatccg
     5581 ttatcgcgag tacgggataa ctgtatttca ctgttgcggg gagtgatgag tgagcgtggc
     5641 gttcagcaaa aatcactggc ggccacactt gaagaattac agcggatttg cgacagcctt
     5701 gcccgtcatc atcaacctgc cgcccgtgag ctggcggcaa ttgtctggcg gctgtactgc
     5761 tcgctttcgc aacttgagca agcaccaccg caaggtacgc tggcctcgta attacttaat
     5821 tacaccacag gcatagcgtt caccgccacc gcccagcggt ttaggttgat cggacatatt
     5881 atcgccgcca acgtggacca tcagcgcctt gtctttgatt tcatccagtg atttcagacg
     5941 aggcgcgatg acggcatcgg tagctttgcc gtcattattg acgaccagtg caggcagatc
     6001 gcctaaatgc ccggtacctt ctggcccttc atgtttaccg gtattttgtg gatcaagatg
     6061 cccgcctgcg gattccgcgg cgctggcttt gccatctttg gtggctggct ggcagcttcc
     6121 tttggcatga atatggaagc catgttcacc ggggggtaat gctttcagat cgggtgaaaa
     6181 ctccagacct ttatcggttt cagtaatggt gacgctacca attgactgcc caaccccttg
     6241 cgacgtgacg aggttcatct cgactttttc actggcagct tgtgcgccgg tcgcaacaac
     6301 cagcgccaga atggccagac taaaacgttt cataggacct ccgttcattg tgcattcctg
     6361 aataagtgta aaccagtgac acatttttga acggcggcta ttcctaaaag tgcttacggt
     6421 acgtcgtacc ccagtgcagc tttacggata cgaaaccatt gttgacgggt cattttcagt
     6481 gtttctgctt cgacagctgc ccgtacgcgc tcaattttac ctgagccgat aattggcagc
     6541 ggctgcgacg gtaaacgtaa tacccaggcg taaaccacct gttcaatcga gcccgcgttt
     6601 aactcctccg ccaccacagc cagttcatca cgcagcggct ggaaataatc atcattaaac
     6661 agacgaccac caccaaggca ggaccacgcc atcggacgaa cacgcagttg ttgtagttgg
     6721 tcgagcgtgc catccagcag taacggctga tgcaccgggg atatttccac ctgattagtg
     6781 gcaagggtaa acggcagacg tgattgcaac agggcaaatt gcgcaggcgt aaagttcgat
     6841 acgccaaaat gacgcacttt gccgctctga tgcagatgtt tgaacgcgtc cgccacttca
     6901 tcggcatcca ttaacgggtc tggtcggtgg attaacagca gatccagatg atcggtcgcg
     6961 agattaatta gcgactgttc ggcgctctta atgatgtgat tgcggtcagt gatgtaatga
     7021 ccaatgacgt tttcttcacg cgcggtcgtc gcgataccgc atttactgac gatttccatc
     7081 cgttcacgca ggtgaggtgc cagtttcagt gcctcgccaa acgccgcttc gcactgatag
     7141 ccaccataaa tatcagcatg gtccacggtg gtcacgccga gatccagatg ctcttcaata
     7201 aaactgacca gctggcgggc ggacatattc cagtccatca atcgccagta gcccatcaca
     7261 aaacgggaaa actccgggcc ttccggcgca atagtaatac gctgaaccat aatcgcttcc
     7321 tcttatcaga tataagagga gtatacgcaa gattaggttc aaaagagtga tggttgctcc
     7381 ggttcgtctg atgacgctgg cttatttgcg cgtaatttgc gcattaatcg ctgccgacaa
     7441 aggcgcagca cctcttgttt ttcgccatcg ctcattttat tccagttaaa acgctcatcc
     7501 cgactacgaa aacagccgcg acaaaacccg cgttcgtcag actggcaaat tccccggcac
     7561 gggctctgga cgggaaagaa ctctaattgc tccgccacct cgccctcctc agataagatt
     7621 attaccatta ttgaagctgt taatgtccaa agtagcaact ttgcttgcac tagaccaact
     7681 ggtctactac actccaacgc atgaacaaac acaccgaaca tgatactcgc gaacatctct
     7741 tggcgacggg cgagcaactt tgcctgcaac gtggattcac cgggatgggg ctaagcgaat
     7801 tactaaaaac cgctgaagtg ccgaaagggt ccttctatca ctactttcgc tctaaagaag
     7861 cgtttggcgt tgccatgctt gagcgtcatt acgccgcata tcaccagcga ctggctgagt
     7921 tgctgcaatc cggcgaaggt aactaccgcg accgcatact ggcttattac cagcaaacac
     7981 tgaaccagtt ttgccaacat ggaaccatca gtggttgcct gacagtaaaa ctctctgccg
     8041 aagtgtgcga tctgtcagaa gatatgcgca gcgcgatgga taaaggtgct cgcggcgtga
     8101 tcgccctgct ctcgcaggct ctggaaaatg gccgtgagaa ccattgttta accttttgtg
     8161 gcgaaccgct acaacaggca caagtgcttt acgcactatg gcttggcgcg aatctgcagg
     8221 ccaaaatttc gcgcagtttc gagccactgg aaaacgcgct ggcccatgta aaaaacatta
     8281 ttgcgacgcc tgccgtttag caggcatttt ttatcaccag acgaccggga gcctttatgt
     8341 catctgaaaa actgtattcc ccactgaaag tgggcgcgat cacggcggca aaccgtattt
     8401 ttatggcacc gctgacgcgt ctgcgcagta ttgaaccggg tgacattcct accccgttga
     8461 tggcggaata ctatcgccaa cgtgccagtg ccggtttgat tattagtgaa gccacgcaaa
     8521 tttctgccca ggcaaaagga tatgcaggtg cgcctggcat ccatagtccg gagcaaattg
     8581 ccgcatggaa aaaaatcacc gctggcgttc atgctgaaaa tggtcatatg gccttgcagc
     8641 tgtggcacac cggacgcatt tctcacgcca gcctgcaacc tggcggtcag gcaccggtag
     8701 cgccttcagc acttagcgcg ggaacacgta cttctctgcg cgatgaaaat ggtcaggcga
     8761 tccgcgttga aacatccatg ccgcgtgcgc ttgaactgga agagattccg ggtatcgtca
     8821 atgatttccg tcaggccatt gctaacgcgc gtgaagccgc ttttgatctg gtagagctcc
     8881 actctgctca cggttatttg ctgcatcagt tcctttcccc ttcttcaaac catcgtaccg
     8941 atcagtacgg cggcagcgtg gaaaatcgcg cacgtttggt actggaagtg gtcgatgccg
     9001 ggattgaaga atggggtgcc gatcgcattg gcattcgcgt ttcgccaatc ggtactttcc
     9061 agaacacaga taacggcccg aatgaagaag ccgatgcact gtatctgatt gaacaactgg
     9121 gtaaacgcgg cattgcttat ctgcatatgt cagaaccaga ttgggcgggg ggtgaaccgt
     9181 atactgatgc gttccgcgaa aaagtacgcg cccgtttcca cggtccgatt atcggcgcag
     9241 gtgcatacac agtagaaaaa gctgaaacgc tgatcggcaa agggttaatt gatgcggtgg
     9301 catttggtcg tgactggatt gcgaacccgg atctggtcgc ccgcttgcag ctcaaagctg
     9361 agcttaaccc acagcgtgcc gaaagtttct acggtggcgg cgcggaaggc tataccgatt
     9421 acccgacgtt gtaatccaac attgcgagcg gcgtaaagcc gccgctatac taaaacaaca
     9481 ttttgaatct gttagccatt ttgaggataa aaagatgcgt cttcttcata ccatgctgcg
     9541 cgttggcgat ttgcaacgct ccatcgattt ttataccaaa gtgctgggca tgaaactgct
     9601 gcgtaccagc gaaaacccgg aatacaaata ctcactggcg tttgttggct acggcccgga
     9661 aaccgaagaa gcggtgattg aactgaccta caactggggc gtggataaat acgaactcgg
     9721 cactgcttat ggtcacatcg cgcttagcgt agataacgcc gctgaagcgt gcgaaaaaat
     9781 ccgtcaaaac gggggtaacg tgacccgtga agcgggtccg gtaaaaggcg gtactacggt
     9841 tatcgcgttt gtggaagatc cggacggtta caaaattgag ttaatcgaag agaaagacgc
     9901 cggtcgcggt ctaggcaact aatctcctgc cgggcgtgaa ctcatcgcgc ccgcatcttt
     9961 actgcatcga caagtaatat ttgtcataat gcgcgctgca atttatccgt attaagagaa
    10021 tcagatgtcc gataacgctc aacttaccgg tctgtgcgac cgttttcgtg gtttttatcc
    10081 tgttgtgatc gatgttgaaa cagccggatt taacgccaaa accgatgcgc tgcttgagat
    10141 tgccgccatc accctgaaaa tggatgaaca aggctggctg atgccggaca ccacattaca
    10201 tttccacgtc gaaccatttg tcggcgcaaa tttgcaacca gaagccctcg ccttcaacgg
    10261 cattgacccg aacgatcccg atcgcggcgc ggtcagcgaa tacgaggcgc tgcacgaaat
    10321 ttttaaagtt gtacgtaaag gtattaaagc gagcggctgt aaccgcgcca ttatggtggc
    10381 gcacaacgcc aattttgatc acagctttat gatggccgcc gcagaacgcg cctcgctgaa
    10441 acgtaacccg ttccaccctt tcgccacttt tgacactgct gcactggccg ggctggcact
    10501 cggacaaacc gtattgtcaa aggcttgcca gaccgctggc atggacttcg acagcaccca
    10561 ggcgcactcc gcgctgtacg acaccgaacg cactgctgtg ctgttttgtg aaatcgtcaa
    10621 ccgctggaaa cgtctgggag gctggccgct acctgccgcc gaagaggtgt aatcgagtcg
    10681 atgcctgatg acatgcaatg attcaggcat ctatagtgag gctattccac gcatcaagca
    10741 tgatattcac ggggaatagc gttaatggca gataatccag acccttcatc gctcctgccg
    10801 gacgtgtttt caccggcgac ccgcgactgg tttcttcgcg cctttaaaca gccgaccgct
    10861 gtccagtcgc aaacctggca tgtggcggcg cgaggcgaac atgcgctggt gattgcaccg
    10921 actggctccg ggaaaacgct ggcagcattt ctctacgccc tcgatcggct cttccgcgaa
    10981 ggcggcgaag atacccgcga ggcgcataag cgtaaaacct cacgcatcct ctatatttca
    11041 ccgataaaag ccctgggcac cgacgttcag cgcaacttgc agatcccgtt gaagggtatt
    11101 gccgatgaac ggcggcggcg cggcgaaacg gtaattattg atgaagtcca cgcggtagcg
    11161 ggcagtaaac gtggtgcgca tctggcgtta agtctggagc ggctcgatgc gctgctccac
    11221 acctcagcac agcgaattgg cctttctgcc actgtgcgct cagccagcga tgtggcagca
    11281 tttcttggtg gcgatcgccc ggttacggta gtcaacccgc ccacaatgcg ccatccgcag
    11341 atacgaattg tcgtaccggt cgccaatatg gatgatgtct catcggtcgc cagcggcacc
    11401 ggcgaagaca gccatgccgg ccgggaaggc tccatctggc catatattga aacgggtatc
    11461 cttgatgaag tgttgcgcca tcgctcgacc attgtcttta ctaattcacg cgggctggcg
    11521 gaaaaactga cggcacgatt aaatgagctt tacgccgcac gcttacagcg ttccccgtct
    11581 atcgccgttg atgcggccca tttcgagtcg acctccggcg caacctctaa ccgtgtacaa
    11641 agtagcgacg tttttattgc ccgctcacac cacggctccg tctctaaaga acaacgagca
    11701 atcaccgaac aggcgctgaa atcgggtgaa ttacgctgcg tggtcgcaac ctccagtctt
    11761 gaactgggga ttgatatggg cgcggtggat ctggtgattc aggtggcaac gccgctttct
    11821 gttgccagtg ggttacaacg cattggtcgc gccggacatc aggttggcgg tgtatctaaa
    11881 gggctgtttt tcccccgtac ccggcgtgat ttagtcgatt ccgcagtcat tgtagagtgt
    11941 atgttcgcag gcaggctgga aaacctgaca ccaccgcata atcctctcga cgtccttgcg
    12001 cagcaaaccg ttgccgccgc ggcgatggat gcattacagg tagacgaatg gtactcccgc
    12061 gtacgccgta ccgcaccgtg gaaagatctg ccaagacgtg tttttgacgc cacgctggat
    12121 atgctttccg ggcgctatcc ctctggcgat ttttctgctt ttcgccccaa actggtctgg
    12181 aacagggaga ccgggatatt gaccgcccga cctggcgctc aattgttggc ggttaccagc
    12241 ggcggcacca ttccggatcg tggcatgtat agcgtgttat tacccgaagg tgaagaaaag
    12301 gccggttcgc ggcgggtggg tgaactggat gaggagatgg tatatgagtc gcgggtgaac
    12361 gacattatca ctctcggcgc tacctcatgg cggatccagc aaatcacccg cgatcaggtg
    12421 attgtgactc ctgctccggg tcgttctgcc cagctcccct tctggcgtgg tgaaggtaac
    12481 ggacgtccgg ctgaattagg cgagatgatc ggcgattttc ttcatttact ggcggatggc
    12541 gcgttctttt ccgggactat tcccccgtgg ctggcagaag aaaatacgaa cgccaatatt
    12601 caggggttga ttgacgagca acgcaacgcg acgggcatcg ttccggggag tcgccatctg
    12661 gtcctcgaac ggtgccgtga tgaaattggc gactggcgta ttattttgca ctctccctat
    12721 ggaagacggg tgcatgaacc ctgggcgctg gcgattgccg ggcgaataca tgccctatgg
    12781 ggcgctgacg cgtctgtggt cgccagtgat gacagcattg ttgcgcgcat tcctgacact
    12841 gatggtaaat tgcccgacgc cgcgattttt ttgttttaac cagaaaagtt gctgcaaatt
    12901 gtccgcgagg cggtaggcag ctcggcactt ttcgccgccc gttttcgcga atgcgccgcg
    12961 cgggcattat taatgccggg ccgcacgccg ggccatcgca ccccgctttg gcaacaacgg
    13021 ctgcgcgcca gtcagttgct ggaaatcgct cagggatatc cggattttcc ggtcattctc
    13081 gaaaccctac gcgaatgtct gcaagatgtt tatgatcttc ccgcactgga acgtttgatg
    13141 cgtcgcctga acggtggcga aattcaaata tccgatgtaa cgaccactac gccctcgcct
    13201 ttcgccacaa gtttattgtt cggctatgtc gcggaattta tgtaccagag cgacgccccg
    13261 ctggcagagc gccgggcatc cgtactgtcg ctggacagcg agttactgcg caatctactc
    13321 ggacaggtcg atccggggga attactcgac ccgcaggtca ttcgccaggt ggaagaagag
    13381 ttgcaacgac tggctcctgg tagaagagcg aaaggtgaag aaggattgtt cgacctgctg
    13441 cgcgaactgg ggccaatgac cgttgaagac ctggcgcaac ggcatacagg cagcagtgaa
    13501 gaggttgcgt cgtatctgga aaatcttctt gcagtaaaac gaatcttccc agcgatgatt
    13561 agcggacagg agcgtcttgc ctgtatggat gatgccgcca ggctgcgtga tgccctcggc
    13621 gtacggctac cagagtcatt gccagagatt tatttacata gagtcagtta cccgcttcgc
    13681 gacctctttc tgcgctatct ccgggctcat gctctggtca cggctgaaca actggctcat
    13741 gagtttagtc tcggtattgc cattgtcgaa gagcagcttc agcaactgcg tgaacagggt
    13801 ctggtgatga atctgcaaca agacatctgg gtgagcgatg aagtatttcg tcgtctgcgt
    13861 ttgcgctcgc tgcaagccgc cagagaagcg acgcgtcccg ttgcagccac gacctatgcg
    13921 cgattgctgc tggaacgtca gggcgtatta cccgccaccg atggtagccc ggcgctcttt
    13981 gcctcaacat ctccaggcgt ttatgagggc gtagatggag tgatgcgggt gatcgaacag
    14041 cttgccggag tcggtttacc cgcctcactc tgggaaagcc agatcctgcc tgcccgcgta
    14101 cgcgactatt cgccagaaat gctcgatgaa ttactggcaa ccggagcggt tatctggtcg
    14161 ggacaaaaaa aagctgggtg aagatgacgg cctggtggca ctgcatctac agaaatatgc
    14221 cgcagaatcg ttcactcccg ccgaagcgga tcaggcgaat cgttcggcgc tgcagcaagc
    14281 gataatcgct gttctggctg acggaggagc ctggtttgca caacaaatca gccaacggat
    14341 acgcgataaa atcgtcgaat cggttgattc ctcggcactg caagaggcgt tatgggcgct
    14401 ggtctggcaa ggcgtcatta ccagcgatat ttgggcaccg ttacgcgccc tcacccgcag
    14461 cagttccaac gcacgcacct caactcgccg cagtcaccgg gctcgtcgtg ggcgtcctgt
    14521 ctatgcacaa cccgtctcgc cgcgggtatc ttacaacaca ccaaatctgg ctggacgctg
    14581 gtcgttattg caggtggagc cactaaacga taccgaaagg atgctggcgc tggcggaaaa
    14641 tatgctcgac cgctacggca tcatcagtcg tcaggcggtg atagccgaaa atatccctgg
    14701 cgggtttcca tcgatgcaaa cactttgtcg aagtatggaa gactccgggc gaattatgcg
    14761 aggtcgtttt gtagaaggtc tgggtggcgc gcaattcgct gaacgtctga ctattgaccg
    14821 attgcgcgat ctggcgacac aagccacgca aacacgccac tatacaccag tggcgctctc
    14881 cgccaacgat ccggctaatg tgtggggaaa tcttctgccc tgggctgcac atccggcaac
    14941 gctggtacca acgcgtcggg cgggtgcgct ggtggtcgtt tctggcggca aattgttact
    15001 ctatctggcg caaggcggca aaaaaatgct ggtctggcag gaaaaggagg aattactcgc
    15061 cccagagatt ttccaggcgc tgaccaccgc actgcgtcgc gaaccaaggc tgcgctttac
    15121 gctaacagaa gtgaatgatc ttccggtccg gcaaacgccg atgtttacgc tgctgcgtga
    15181 ggcgggattt tcaagttcgc cacaagggct ggattgggga tagttgacat cctccacgcc
    15241 ctgaaagacg tggttccctg ctacgttcaa gctgtcgcct gaatcatttc ggtgggttcc
    15301 tgcttcaacg agcggcctga ctgcaccatc tctccacagg caagaacggc gtgccccgcc
    15361 gctaaaatgt tacgagcgcc atttacatcg gcgttcgctg tatatccaca tacctggcat
    15421 ctgaatttac tttgtgacag gcggttttct ttcgctgtat gaccacagca cgcgcaacgc
    15481 tggcttgtgt acgctggcgg aacagcaagc acctgaccgc cactccagag ctgcttgtac
    15541 tcaagctggc ggcgcatttc ataccagccc tgatccagta tcgaacggtt taaacctgat
    15601 tttgcccgga cattgcgccc cggctggctg accgtacccg ctgctgactt tgacatgttg
    15661 ctgaccttca aatcctcaat gactatcatt gcgtggtttt tgctgacggt cgttgtgact
    15721 ttgtgaaggt agtccctgcg gatatttgcg atacaggaat gcagtcgctg tattttgcgt
    15781 ttctgcttct gccagttgtt gctgaatttg accttccggc ttaactgtcg ctgaagtctc
    15841 gccagcgtct tctggttttt ttgaaaactg tttacaggct caaagactgt gccatctgac
    15901 agcgtggcga gtttagccac gccagcatcc agcccgatca ttgatgctga agggtgaacc
    15961 ggagttgata cttcactttc tgtctgaata ctgatgtacc acgtaccgca ggactggctg
    16021 acagtgacat ttttcacaac tcccgtgacc tgacggctat tccggtagcg catccagcca
    16081 agtttcggca gaaaaatacg gctgttttcc tgatcgagct taacgtcctg cgggtagcgg
    16141 aatgcagcat tctgtccccg ctttttgaat cggggaaaag cagcccgctt ctggaagaag
    16201 tttttgtaag cccgctcaag gtctttcagt gactgttgca atggctgtga gggcgaatct
    16261 ttaagccatt gcgtttcagt ggcgtttttc cactcaacca gccaggaagc cattttcccg
    16321 taagggatgt atttattacc ggcctcatga ttctcattct gaagcgccag agcacgattg
    16381 aaaacgaaac gacatgcgcc tgcgaagcgc ctcatttcac gctcct
//
LOCUS       DAJTSA010000087        16389 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000087 DAJTSA010000000
VERSION     DAJTSA010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.087"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(87..2063)
                     /gene="speA"
                     /locus_tag="OO369_003025"
     CDS             complement(87..2063)
                     /gene="speA"
                     /locus_tag="OO369_003025"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR5983211.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="OO369_003026"
     CDS             complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="OO369_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR5983212.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2339..2554
                     /locus_tag="OO369_003027"
     CDS             2339..2554
                     /locus_tag="OO369_003027"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983213.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2858..4012
                     /gene="metK"
                     /locus_tag="OO369_003028"
     CDS             2858..4012
                     /gene="metK"
                     /locus_tag="OO369_003028"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR5983214.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4449..5843
                     /gene="galP"
                     /locus_tag="OO369_003029"
     CDS             4449..5843
                     /gene="galP"
                     /locus_tag="OO369_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR5983215.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5920..6417
                     /locus_tag="OO369_003030"
     CDS             5920..6417
                     /locus_tag="OO369_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR5983216.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6512..7219
                     /gene="endA"
                     /locus_tag="OO369_003031"
     CDS             6512..7219
                     /gene="endA"
                     /locus_tag="OO369_003031"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR5983217.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7299..8030
                     /gene="rsmE"
                     /locus_tag="OO369_003032"
     CDS             7299..8030
                     /gene="rsmE"
                     /locus_tag="OO369_003032"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR5983218.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8043..8993
                     /gene="gshB"
                     /locus_tag="OO369_003033"
     CDS             8043..8993
                     /gene="gshB"
                     /locus_tag="OO369_003033"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR5983219.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9102..9665
                     /locus_tag="OO369_003034"
     CDS             9102..9665
                     /locus_tag="OO369_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR5983220.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9665..10081
                     /gene="ruvX"
                     /locus_tag="OO369_003035"
     CDS             9665..10081
                     /gene="ruvX"
                     /locus_tag="OO369_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR5983221.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10257..11237)
                     /locus_tag="OO369_003036"
     CDS             complement(10257..11237)
                     /locus_tag="OO369_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR5983222.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            11255..11959
                     /gene="yggS"
                     /locus_tag="OO369_003037"
     CDS             11255..11959
                     /gene="yggS"
                     /locus_tag="OO369_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR5983223.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11977..12543
                     /gene="yggT"
                     /locus_tag="OO369_003038"
     CDS             11977..12543
                     /gene="yggT"
                     /locus_tag="OO369_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR5983224.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12540..12830
                     /locus_tag="OO369_003039"
     CDS             12540..12830
                     /locus_tag="OO369_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR5983225.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12838..13431
                     /locus_tag="OO369_003040"
     CDS             12838..13431
                     /locus_tag="OO369_003040"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR5983226.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13424..14560
                     /gene="hemW"
                     /locus_tag="OO369_003041"
     CDS             13424..14560
                     /gene="hemW"
                     /locus_tag="OO369_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR5983227.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14874..15837
                     /locus_tag="OO369_003042"
                     /pseudo
     CDS             14874..15837
                     /locus_tag="OO369_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15882..16385
                     /locus_tag="OO369_003043"
     CDS             15882..16385
                     /locus_tag="OO369_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR5983228.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4059 a         4220 c         4124 g         3986 t
ORIGIN      
        1 agcagctaga ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa
       61 tcgacccgcc agcggaataa tcttaattat tcatcttcaa gataagtata accgtacaaa
      121 cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc
      181 acttgatcgc ggaactgggt taacagcgtt ttcggatcga gctgtacata ttgcagcatg
      241 tccgccacgg tatcgccttc gtcagacagt tctacttcta cgctaccgtc agggaacacg
      301 aacacgtcaa ctgcttcggt atcaccgaac aggttgtgca tattgccaag gatctcctga
      361 tatgcaccga ccataaagaa accgagcatc ggcggattct ctggatcgta ctccggcatt
      421 ggcattgtcg tggcaatacc atcaccatca atatagtggt cgatagcacc gtcagagtca
      481 caggtaatat ccagcagcac agcgcgacgt tccggcactt gatccagccc ttccagcggt
      541 agaaccggga acaactggtc gatcccccat gcgtccggca ttgactggaa cagcgagaaa
      601 ttgacgtaca ttttgtccgc catacgttcc tgcagttcgt cgattatcgg acgatgcgca
      661 cggttttgcg gatccagttg cttctgcact tcatggcaca tgctcagata gagttgttct
      721 gcccatgcac gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatgtcgtgc
      781 agatccatct ggctgtcgtg taaccattca cgcaaagaac ggcgagttcc cggttcgtgc
      841 atttcctgcc aggtttccca catgctttgc agcgcacgcg gcgcatcttc tacgggcgcg
      901 gtcggcaccg tatattcgtt acgttccacg ccgatgatat tcgacaccag cacggtatgg
      961 tgcgcagtca ccgcacggcc cgattcggtg attaccgtcg gatgcggcag accattttct
     1021 tcacacgcat cgccaatcgc ccagatgatg ttgttggcgt attcattgag gccatagttt
     1081 acggagcagt cagactgcga acgggtgcct tcataatcca cgcccagacc gccgccgacg
     1141 tcgaagcact gaatattgac gcccagcttg tgcagttcca catagaaacg cgcggattca
     1201 cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagt
     1261 agttgcaggc tgtcgagacg cccggcttca cgcagggttt caaccagttg cagtacctga
     1321 gtcgcagcca ggccgaactt cgatttttcc ccgccggagg actgccattt acccgagccc
     1381 tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca
     1441 tccagcacaa tggcgatttc tgacatcttc tcaatgacca gatagacctt gtgccccatc
     1501 ttctcgccaa ttaatgccag gcggatatat tcgcggtctt tataaccgtt gcagacgatg
     1561 acgctacgag tcatgccagc atgtgccagc actgccatca actcggcttt ggaaccggct
     1621 tccagaccca gcggttcacc cgaatgaatc agggactcga tcacgcggcg gtgctggtta
     1681 actttgatcg gataaacaag gaagtaatcg ccgttatagc cgtaggattc cctcgcacgt
     1741 ttgaacgcgg cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt
     1801 gcaggcagac gctgaccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga
     1861 gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag
     1921 tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag
     1981 ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa
     2041 cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt
     2101 taacgactgt cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca
     2161 gtgtatgctg acgctccgac tgcgcaaccg gtttcttttt cataacatta ttaagcacat
     2221 aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag
     2281 cgcccttgtt cagtcctcag taactgtaac cagctcttga atcctgagaa gcgccgagat
     2341 gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac gagtcttcac
     2401 cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg
     2461 gccgcctgtt aagacgaacc cacaagccaa aactctgatt tcaacccggc tggaagtggc
     2521 aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac
     2581 aatggcagaa aattgcaaaa gataaatacg cagaatgccg gcattgtcag gaaaaatttc
     2641 cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc gggtgaagaa
     2701 ttgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc
     2761 agtgttttta acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag
     2821 tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc
     2881 cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt
     2941 agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac
     3001 cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat
     3061 cacccgtaac accgttcgcg aaattggcta tgtgcattcc gacatgggct ttgacgctaa
     3121 ctcctgtgcg gttctgagcg ctatcggcaa acagtctcct gacatcaacc aaggcgttga
     3181 ccgtgccgat ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac
     3241 taatgaaacc gacgtgctga tgccagcacc tatcacctat gcacaccgtc tggtacagcg
     3301 tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca
     3361 ggtgactttc cagtatgacg acggcaaaat cgttggtatt gatgctgtcg tgctttccac
     3421 tcagcactct gaagagatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat
     3481 caagccaatt ctgcccgctg aatggctgac ttctgccacc aaattcttca tcaacccgac
     3541 cggtcgtttc gttatcggtg gcccaatggg tgactgcggt ctgactggcc gtaaaattat
     3601 cgttgatacc tacggcggca tggcgcgtca cggtggcggt gcattctctg gtaaagatcc
     3661 atcaaaagtg gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa acatcgttgc
     3721 tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc
     3781 gacctccatc atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct
     3841 gctggtacgt gagttcttcg acctgcgccc atacggactg attcagatgc tggatctgct
     3901 gcacccgatc tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac atttcccgtg
     3961 ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt aatctttctt
     4021 cacctgcgtt caaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggat
     4081 aggcgttcac gccgcatccg gcaaaaaaac tgcccgcaca ataacatcat tcttcctgat
     4141 cacgtttcac ggcaaattac catcacaact gaaaccgatt acaccaacca caccagcaaa
     4201 atatttgtaa tattttcata ttattattca gctttcacag ttgttacatt tcttttcagt
     4261 aaagtcttaa ttgcagatga cagcgtttaa tctatgatga tataactcaa ttattttcat
     4321 gcccttaaat cataactaag ataaatgtta gtgtaagcga ttacactgat gtgatttgct
     4381 tcacatcttt ttacgtcgta ctcacctatc ttaattcaca ataaaaaata actatattgg
     4441 agggcatcat gcctgacgct aaaaaacagg ggcggtcaaa caaggcaatg acgtttttcg
     4501 tctgcttcct tgccgctctg gcgggattac tctttggcct ggatatcggt gtaattgctg
     4561 gcgcactgcc gtttattgca gatgaattcc agataacttc gcacacgcaa gaatgggtcg
     4621 taagctccat gatgttcggt gcggcagtcg gtgcggtggg catcggctgg ctctccttta
     4681 aactcgggcg caaaaagagc ctgatgatcg gcgcaatcct gtttgttgcc ggttcactgt
     4741 tctctgcggc tgcgccaaac gttgaagtac tgattctttc gcgcgttctg ctggggctgg
     4801 cggtgggtgt ggcctcttat accgcaccac tgtacctgtc agaaattgcg ccggaaaaaa
     4861 ttcgcggcag tatgatctcg atgtatcagt tgatgatcac tatcgggatc ctcggtgctt
     4921 atctttctga taccgccttc agctacaccg gtgcatggcg ctggatgctg ggtgtgatta
     4981 tcatcccggc aattttgctg ctgattggtg tcttcttcct gccagacagc ccacgttggt
     5041 ttgccgccaa acgccgtttt gttgatgccg aacgcgtgct gctacgcctg cgtgacacca
     5101 gcgcggaagc gaaacgcgaa ctggatgaaa tccgtgaaag tttgcaggtt aaacagagtg
     5161 gctgggcgct gtttaaagag aatagcaact tccgccgcgc ggtgttcctt ggcgtactgt
     5221 tacaggtaat gcagcaattc accgggatga acgtcatcat gtattacgcg ccgaaaatct
     5281 tcgaactggc gggttatacc aacaccaccg agcaaatgtg ggggacagtg attgtcggcc
     5341 tgaccaacgt acttgccacc tttatcgcaa tcggccttgt tgaccgctgg ggacgtaaac
     5401 caacgctaac gctgggcttc ctggtgatgg ctgctggcat gggcgtactc ggtacaatga
     5461 tgcatatcgg tatccactct ccgtcggcgc agtatttcgc catcgccatg ctgctgatgt
     5521 ttattgtcgg ttttgccatg agtgccggtc cgctgatttg ggtactgtgc tccgaaattc
     5581 agccgctgaa aggccgcgat tttggcatca cctgctccac cgccaccaac tggattgcca
     5641 acatgatcgt tggcgcaacg ttcctgacca tgctcaacac gctgggtaac gccaacacct
     5701 tctgggtgta tgcggctctg aacgtactgt ttatcctgct gacattgtgg ctggtaccgg
     5761 aaaccaaaca cgtttcgctg gaacatattg aacgtaatct gatgaaaggt cgtaaactgc
     5821 gcgaaatcgg cgctcacgat taatctcctc aggcttcctc ccgtcgcgga ggaagccgcc
     5881 tcttgcagtc atcttttctt cgctctatcc tctgccgcta tgaaaacatc ccgtctccct
     5941 atcgccatcc aacaggccgt tatgcgccgc ctgcgggaaa aactcgccca ggccaacctg
     6001 aagctagggc gtaactaccc ggagccaaaa ctctcttaca cccagcgcgg aacctccgcc
     6061 ggaacagcct ggctggaaag ctatgaaatc cgcctcaatc ccgttttgct gttggaaaac
     6121 agtgaagctt ttattgaaga agtggtaccg cacgaactgg cacatttgct ggtatggaaa
     6181 catttcggcc gcgtagcgcc acatggcaaa gagtggaagt ggatgatgga aagcgtgctg
     6241 ggcgttcccg cccgtcgtac gcatcagttc gaactgcaat ccgtgcgtcg caacaccttc
     6301 ccctaccgct gtaagtgcca ggaacatcag cttaccgtac gccgccataa tcgcgtagtt
     6361 cgtggcgagg ccatctatcg ctgtgttcac tgcggtgaac agctggttgc gaaataacca
     6421 cctgaactat caggaacttt cctgatctgg ctgattgcat accaaaacaa ctttcgctac
     6481 gttgctggct cgttttaaca cggagtaagt gatgtaccgt tatttgtcta ttgctgcggt
     6541 ggtactgagc gcagcatttt ccggcccgac gttggccgaa ggtatcaata gtttttctca
     6601 ggcgaaagcc gcggcggtaa aagtccacgc tgatgcgccc ggtacgtttt attgcggatg
     6661 taaaattaac tggcagggca aaaaaggcgt tgttgatctg caatcgtgcg gctatcaggt
     6721 gcgcaaaaat gaaaaccgcg ccagccgcgt agagtgggaa cacgtcgttc ccgcctggca
     6781 attcggtcac cagcgccagt gctggcagga cggtggacgt aaaaactgcg ctaaagatcc
     6841 ggtctatcgc aagatggaaa gcgatatgca taacctgcag ccgtcagtcg gcgaggtgaa
     6901 tggcgatcgc ggcaatttta tgtacagcca gtggaatggc ggcgaaggcc agtacggtca
     6961 atgcgccatg aaggtcgatt tcaaagaaaa agctgccgaa ccaccagcgc gtgcacgcgg
     7021 tgccattgcg cgcacctact tctatatgcg cgaccaatac aacctgacac tctctcgcca
     7081 gcaaacgcag ctgttcaacg catggaacaa gatgtatccg gttaccgact gggagtgcga
     7141 gcgcgatgaa cgcatcgcga aggtgcaggg caatcataac ccgtatgtgc aacgcgcttg
     7201 ccaggcgcga aagagctaac ctacactagc gggattcttt ttgttaaccc ctaccccacg
     7261 cgtacaaccg cgtggggaga cgacgcggat ttttaactat gcgtatcccc cgcatttatc
     7321 atcctgaacc actgaccagc cattctcaca tcgcgctttg cgaagatgcc gccaaccata
     7381 tcgggcgcgt actgcgcatg gggccggggc aggcgttgca attgtttgac ggtagcaacc
     7441 aggtctttga cgccgaaatt accagcgcca gcaaaaaaag cgtggaagtg aaggtgctgg
     7501 aaggccagat cgacgatcgc gaatctccgc tgcatattca cctcggtcag gtgatgtcgc
     7561 gtggtgaaaa aatggaattt actatccaga aatcgatcga actcggtgta agcctcatta
     7621 cgccactttt ttctgagcgc tgcggcgtta aactggatag tgaacgtctg aacaagaagc
     7681 ttcagcagtg gcagaagatt gcaattgctg cctgtgagca gtgtggtcgt aaccgggtgc
     7741 cggaaatccg tccagcgatg gatctggaag cctggtgtgc agagcaggat gaaggactga
     7801 aactgaatct tcacccgcgc gccagtaaca gcatcaatac gttgccttta ccggttgaac
     7861 gcgtccgcct gctgattggc ccggaaggcg gtttatcggc agatgaaatt gccatgactg
     7921 cccgctatca atttactgat atcctgttgg gacctcgcgt tttgcgtaca gagacaactg
     7981 cgctcaccgc cattaccgcg ctacaagtac gatttggcga tttgggctaa cggagaagaa
     8041 taatgatcaa gctcggcatc gtgatggacc ccatcgcaaa catcaacatc aagaaagatt
     8101 ccagtttcgc tatgttgctg gaagcacagc gtcgtggtta cgaacttcac tatatggaga
     8161 tgggcgatct gtatctgatc aatggcgaag cccgcgccca tacccgcacg ctgaacgtga
     8221 agcagaacta cgaagagtgg ttttcgttcg tcggtgaaca ggatctgccg ctggccgatc
     8281 tcgatgtgat cctgatgcgt aaagacccgc cgtttgatac cgagtttatc tacgcgacct
     8341 atattctgga acgtgccgaa gagaaaggga cgctgatcgt taacaagccg cagagcctgc
     8401 gcgactgtaa cgagaaactg tttaccgcct ggttctctga cttaacgcca gaaacgctgg
     8461 ttacgcgcaa taaagcgcag ctaaaagcgt tctgggagaa acacagcgac atcattctta
     8521 agccgctgga cggtatgggc ggcgcgtcga ttttccgcgt gaaagaaggc gatccaaacc
     8581 tcggcgtgat tgccgaaacc ctgactgagc atggcacttg ctactgcatg gcgcaaaatt
     8641 acctgccagc cattaaagat ggcgacaaac gcgtgctggt ggtggatggc gagccggtac
     8701 cgtactgcct ggcgcgtatt ccgcaggggg gcgaaacccg tggcaatctg gctgccggtg
     8761 gtcgcggtga acctcgtccg ctgacggaaa gtgactggaa aatcgcccgt cagatcgggc
     8821 cgacgctgaa agaaaaaggg ctgatttttg ttggtctgga tatcatcggc gaccgtctga
     8881 ctgaaattaa cgtcaccagc ccaacctgta ttcgtgagat tgaagcagag tttccggtgt
     8941 cgatcaccgg aatgttaatg gatgccatcg aagcacgttt acagcagcag taacccacct
     9001 tagcgagaag gatctcgttg agactctgag tgacagcgcc cttctttcca cgcatactgg
     9061 gcgctgttgc ttttttgaac caggaaacag aacctctgac aatgaattta cagcatcact
     9121 ttcttattgc catgcctgct ctccaggatc cgattttccg tcgttccgtg gtctacattt
     9181 gcgaacataa taccaatggt gcaatgggga tcatcgtcaa caagccgctg gaaaatctca
     9241 aaattgaagg gattctggaa aagctgaaga tcacgccgga gccgcgtgat gaatcaatcc
     9301 gtctggataa accggttatg ctcggcggcc cactggctga agatcgcggg tttattttgc
     9361 atactccgcc ctccaatttt gcttccagca ttcgcatttc agacaacacg gtgatgacca
     9421 cttcccgcga tgtgctggaa acgctcggca ccgataaaca accgtctgac gtattggtgg
     9481 ctctgggcta tgcctcctgg gaaaaaggtc aactggaaca agaaattctc gataacgcgt
     9541 ggctaacggc cccggcggat ctgaatattc tgttcaaaac gccgattgcc gaccgctggc
     9601 gcgaggcggc aaaactgatt ggtgtggata ttctcaccat gcctggtgtg gcaggacacg
     9661 cctgatgagt ggaaccttac tcgccttcga cttcggcacc aaaagcattg gcgtagcggt
     9721 cggccaacgc attaccggca ccgctcgccc tttgcctgca attaaagcgc aggacggtac
     9781 gccggactgg aacctcatcg aacgtttact gaaagagtgg cagcctgacg aaatcatcgt
     9841 cggtttgccg ctgaatatgg acggcaccga acagccgtta accgccagag cgcgcaaatt
     9901 tgccaaccgt attcatggtc gtttcggtgt tgaagtaaag ctccatgacg agcgtcttag
     9961 cactgtggaa gcccgttccg gtctttttga acagggcggc tatcgggcgc tcaacaaagg
    10021 caaagttgac tctgcctctg cggttattat tctcgaaagc tatttcgagc agggatatta
    10081 aggcgatcta aaagcctggc ggatagtcgt taccgccatc ttgccggatg cggcgtgaac
    10141 gccttatcca gcacacatct ggcagcggct gtaggtctga taagacgcgt tagcgtcgca
    10201 tcagacattt attgccttta taggccctat aaacttgcgc atcgggcatg gcaacgtcac
    10261 aaacgccctt cccccacccg ctgctgataa ctctgctgaa acgttaacat ccccaccttc
    10321 tgcccggttt gaataacatg cggtaactgg tgggttttcc cttcgcgaat caaattcccc
    10381 accgcgggag tgttaatcag taattcaaac agcgccacgc gtccttcctg tttatccact
    10441 tccagctttt gtgacagcac tgcccgtaaa ctacccgcaa gttgattacg tacgggatct
    10501 ttttcctgcg ccggaaatga atccaccagt cgctcaactg cctgcgcggc accacgtgta
    10561 tgtaaggttg ccagcaccag atgcccggtt tctgccgccg tcagcgccag acggattgtc
    10621 tcactgtcac gtagctctcc gagcaaaatc acatcaggat cttcccgcaa tgcagcccgc
    10681 aaccccgatg cgaacgtcat acagtgcaaa ccaatttccc gctgctggat caaacatcgc
    10741 tggctggcat agagatattc cacaggatct tccagcgtca gaatatgcgc atcggcatgt
    10801 tgattaagat agccaaccat cgccgccagc gtggtagatt tgccactccc cgtagccccc
    10861 gtcaccagaa tcaggccatt ctcgctcttg agtaattccg gcaataccgg tggtgcacca
    10921 agctgttcga gctgtggaca gtgcgaaggt aacaaccgta atgccagaga aataccttgc
    10981 cgttgcgcga acgcactgcc acgcaaccgc tggttttccg ccagcgacac ggcaaaatcc
    11041 agctggccat tttccagcaa tattgcccgc tgatcgtcat ccagccactc ccgcagtagc
    11101 tcttcgacgt ccggcgcatc aaacggcgca gcttccattc tgccacgaat gcgccatcgt
    11161 gcgggccagg cgctgcacag gtgtagatcc gagacgttat gctttacact aagggccaca
    11221 atttcttcca tattcatact aagatcctcg gaaaatgaac gatattgcgc ataacctggc
    11281 acaggtccgg gacaaaatct cagcggctgc aacgcgttgc ggccgttctc cagaagaaat
    11341 tacgctgctt gcagtcagta aaacaaaacc tgcgagcgcc atcgcagaag ccattgatgc
    11401 cgggcagcgt caatttggtg aaaactacgt tcaggaaggg gtagataaaa ttcgccactt
    11461 tcaggaactg ggcgtaacag gattagaatg gcattttatt ggcccgttgc agtctaataa
    11521 aagccgccta gtggcagagc atttcgactg gtgtcatacc atcgaccgtt tgcgcattgc
    11581 tacccgcctc aacgagcagc gcccggcaga acttccccct cttaacgtac tgattcaaat
    11641 taatattagt gatgaaaaca gtaagtccgg gattcaactg gcagaactgg acgagctggc
    11701 agcggcggtc gctgaactac cgcgtttacg tctgcgcggg ctgatggcaa tccctgcgct
    11761 tgagtcagaa tatgtaaggc agtttgaagt tgcacgccaa atggctgtag catttgccgg
    11821 actgaaaacg cgctacccgc atatcgacac gctctctctg ggaatgtcgg acgatatgga
    11881 agccgccatt gcggcaggta gcacgatggt tcgtatcggc actgcaattt ttggtgcgcg
    11941 tgattactct aaaaaataag gaattaaagg aacgccatga atacgttgac tttcctgctt
    12001 tcaacggtca ttgagctgta tacaatggtg ctgttattac gcatctggat gcagtgtgct
    12061 cattgtgatt tttacacccc cttctcacag tttgtagtga aggtagcgca gccaattatc
    12121 gggccactgc gccgcgttat tccggcaatg ggtcccattg acagcgcctc gctgctggtt
    12181 gcctatattc tcagttttat caaagccatt gtgctgtttg aagtggtgac cttcctgcca
    12241 atcatctgga ttgccggttt actgattctg ctgaaaacca tcggcttgct gattttctgg
    12301 gtcctgctgg tgatggcgat tatgagctgg gtaagccagg gtcgtagccc gattgaatac
    12361 gtgctgattc agctggccga tccgctgctg cgcccgattc gccgcttgct accggcaatg
    12421 ggtgggattg atttctcgcc gatgatcctc gttctgctgc tgtatgccat caatatgggt
    12481 gtcgcagaag tattacaggc aaccggaaac atgctgctgc cggggctgtg gatggcgtta
    12541 tgagtgccgt aacagttaat gatgacggtc tggttttacg gctctatatt cagccgaaag
    12601 ccagccgtga ttctattgtc ggtttacatg gcgacgaagt taaagtcgcc attaccgcgc
    12661 cgccggttga cggccaggcc aacagtcatc tggtgaagtt tctcggtaag caattccggg
    12721 ttgccaaaag ccaggtggtg attgaaaaag gcgaacttgg ccgccacaaa caaattaaaa
    12781 tcattaatcc gcaacaaatc ccgccagaaa tcgcggcgtt aattaattag gtatcctatg
    12841 caaaaagttg tcctcgcaac cggcaatgcc ggtaaagtgc gtgagctggc gtcgctgctt
    12901 agcgactttg gtcttgatat cgtggcccaa acagacctcg gcgttgattc cgctgaagaa
    12961 accggcctaa cctttatcga aaacgcgatt ctgaaagcgc gccatgcggc aaaagtgacc
    13021 ggtttaccgg caattgccga cgactctggt ctggcggtag atgcgctggg tggcgcgccg
    13081 ggtatttact ccgcgcgtta ttccggtgaa gacgcgaccg atcaaaagaa tctgcaaaaa
    13141 ctgttggaaa cactgaaaga cgtaccggac gaccaacgtc aggcgcgttt ccactgcgtg
    13201 ctggtatatc tgcgtcacgc ggaagatccg actccgctgg tatgccacgg cagctggccg
    13261 ggcgtgatta ctcgcgaacc agcgggcact ggtggctttg gttatgatcc aatcttcttc
    13321 gtaccttccg aagggaaaac cgccgccgaa ctgacccgcg aagaaaagag cgccatttcc
    13381 caccgtggtc aggcgttgaa actgctgctg gacgctttac gtaatggtta aattaccgcc
    13441 gctgagtctc tacattcaca tcccgtggtg cgtgcagaaa tgcccgtact gcgatttcaa
    13501 ctctcacgcg ttgaaaggag aagtgccgca cgacgattat gttcagcatc tgctttgcga
    13561 tctggacaat gatgtggctt acgctcaggg ccgtgaagta aagacaatct ttattggcgg
    13621 tggtacgccg agcctgcttt ccggcccggc gatgcaaacg ctgctagacg gcgtgcgtgc
    13681 gcgtttgccg ctggcagcgg atgcagaaat tactatggaa gcgaacccag gcacggtaga
    13741 agcggatcgc tttgtcgatt atcagcgtgc tggtgtaaac cgcatctcta ttggtgtgca
    13801 gagttttagc gaagaaaagc tgaaacgact tgggcgtatt catggcccgc aagaagcgaa
    13861 acgagcggca aagctggcga gcggtttagg gttacgtagc tttaaccttg atttgatgca
    13921 tggactaccg gatcaatcac tggaagaggc gcttggcgat ctacgccagg ccattgaact
    13981 gaatccgccg catctttcct ggtatcaact gactatcgaa cctaatacgc tgtttggctc
    14041 gcgaccgccg gtgctgccgg acgatgacgc gctgtgggat attttcgaac aggggcatca
    14101 gttattaacc gcagcgggtt atcagcaata tgaaacttcc gcttacgcca aacccggtta
    14161 tcagtgccag cacaatctca actactggcg ctttggtgac tacatcggta ttggctgcgg
    14221 cgcgcacggc aaagtgacct tcccggatgg gcgcattctg cgtaccacta aaacgcgtca
    14281 tcctcgtggt tttatgcaag gaaggtatct ggaaagccag cgtgatgtcg aagccgcaga
    14341 taagccgttt gagttcttta tgaatcgctt ccgtctgctg gaggccgcgc cgcgcgtgga
    14401 gtttattgcg tataccgggc tttgcgaaga tgtgattcgc ccacagttag acgaggcgat
    14461 tgcccagggt tatctcaccg aatgtgcgga ttactggcag ataacggaac atggtaaatt
    14521 attcttaaac tcacttcttg aattattcct cgctgagtaa tcattaatgc gcctgcgggc
    14581 gcattaatca cttcttttat ccccccgtta attaacctcg gcagaaatat atttcttact
    14641 gaataaatat cgacgacatt tattatatat atttagtgaa tatttactaa tcattcattg
    14701 attataaata tgtacaacct ggcgcacaaa tttaatattt aatttctgac tttatttcaa
    14761 gttgtgataa acatagacaa gttgtcagac aggttaacca atttgtcgca caaatcactg
    14821 ataagcacaa ttagatatca accaatttcc atccttcaac agggagtttt tttatgaaaa
    14881 tgaggtcatt tacccgctca ttagtctgcg catccttgct ggctctggtt agcactggcg
    14941 taaacgccgc agaaaaagtg gcgttaaagt tagcacacaa cctggaaaga agtcatgttg
    15001 tacatcagag ttttgaagag ctggcgaaag aggtaaaaca gctgtcaaaa ggtaatatgg
    15061 tgatccgtat ttatcccagc agccagatgg gtaatgcgcg agaaactatg gaattgttgc
    15121 aaaatggcgc actggatatg accaaaggtt ccgccagtga tctggagtct tttgataata
    15181 tttatgctat ctataatttg ccgttcttat taagttgttt ttggtgaagt tggtaaagaa
    15241 attatggact caacaaaaga caaaggcttt tttgctttat cagcttatgt agcgggtact
    15301 cgcagtttct atgcgaaaaa accgattaca aagccagaag atcttaaagg actgaaaatt
    15361 cgtgttcagc caagtccgac caccatcaaa atgattgaac tgatgggtgg ttcacccact
    15421 ccaatttcat ttggcgaagt ttatactgcg atgcagcaag gtgtagtaga tggcgcagaa
    15481 aataacgtgc cttcctggat gcagactcga catattgaaa ttgccaaagt tttttctgaa
    15541 gatgaacacg cctcaattcc tgactttttg gttatctcca ccaaaacatg gaataaatta
    15601 accccagaac agcaacaaat cctcgaaaca gcggcgaaga aatccgaagc ttatcaacaa
    15661 aaattgtggg aaaaaattga cgctgacaca cgtgctcagg cgaaagctat ggggggtgaa
    15721 attgtaaaag tggataaagc gccattccgt aaagccgtgc aacctctgtt tgacgacttc
    15781 aaaaaagatc caaaacaagc tgctttgtta gagaaattcg acaacgctgc tcaataatat
    15841 tccgggccag cctctggccc tgattttcca ggatgcttac aatgatattt aaccgcctga
    15901 aacttgcggt agaccgcgtc attgccacgt tttctgtcgc cgtaatgctt gctctggttg
    15961 tatgcgtagt ctggcaagta tttagccgct acgtacttaa ccagccaagt acattaaccg
    16021 atgagctggc acgctttttg atgatctggg taggattatt aggtgctgca tataccgtcg
    16081 gcgcgcagcg acatctctct attgatttat ttgctctcgc cctgaataag cgcaaacaat
    16141 tacttcttag tatagtcatt aacgttctca ttttaggttt tgccggttcg gtcattgtga
    16201 ctggtggcct gaaattaatt gataaaacac tggcaacatc acaggtatct gccgctatgc
    16261 aaataccgat gggatacgta tatatcattc tgccattaag tggagtggta atgatgtttt
    16321 atgccttgtg ttttattaat caaagcattc aacaactgaa acaacctgcg caggaggcca
    16381 gttaatgga
//
LOCUS       DAJTSA010000088        15944 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000088 DAJTSA010000000
VERSION     DAJTSA010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15944
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.088"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            446..1504
                     /gene="citC"
                     /locus_tag="OO369_003044"
     CDS             446..1504
                     /gene="citC"
                     /locus_tag="OO369_003044"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR5983229.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1519..1815
                     /gene="citD"
                     /locus_tag="OO369_003045"
     CDS             1519..1815
                     /gene="citD"
                     /locus_tag="OO369_003045"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR5983230.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1812..2720
                     /gene="citE"
                     /locus_tag="OO369_003046"
     CDS             1812..2720
                     /gene="citE"
                     /locus_tag="OO369_003046"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR5983231.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2731..4263
                     /gene="citF"
                     /locus_tag="OO369_003047"
     CDS             2731..4263
                     /gene="citF"
                     /locus_tag="OO369_003047"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR5983232.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4267..4831
                     /gene="citX"
                     /locus_tag="OO369_003048"
                     /pseudo
     CDS             4267..4831
                     /gene="citX"
                     /locus_tag="OO369_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4806..5684
                     /gene="citG"
                     /locus_tag="OO369_003049"
     CDS             4806..5684
                     /gene="citG"
                     /locus_tag="OO369_003049"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR5983233.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5735..7197
                     /gene="citT"
                     /locus_tag="OO369_003050"
                     /pseudo
     CDS             5735..7197
                     /gene="citT"
                     /locus_tag="OO369_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7311..8117
                     /gene="rna"
                     /locus_tag="OO369_003051"
     CDS             7311..8117
                     /gene="rna"
                     /locus_tag="OO369_003051"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR5983234.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8346..8756
                     /gene="rnk"
                     /locus_tag="OO369_003052"
     CDS             8346..8756
                     /gene="rnk"
                     /locus_tag="OO369_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR5983235.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8987..10225)
                     /locus_tag="OO369_003053"
     CDS             complement(8987..10225)
                     /locus_tag="OO369_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCR5983236.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10446..10874
                     /gene="uspG"
                     /locus_tag="OO369_003054"
     CDS             10446..10874
                     /gene="uspG"
                     /locus_tag="OO369_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR5983237.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10995..12560)
                     /gene="ahpF"
                     /locus_tag="OO369_003055"
     CDS             complement(10995..12560)
                     /gene="ahpF"
                     /locus_tag="OO369_003055"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR5983238.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12805..13368)
                     /gene="ahpC"
                     /locus_tag="OO369_003056"
     CDS             complement(12805..13368)
                     /gene="ahpC"
                     /locus_tag="OO369_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR5983239.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13740..14486
                     /gene="dsbG"
                     /locus_tag="OO369_003057"
     CDS             13740..14486
                     /gene="dsbG"
                     /locus_tag="OO369_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR5983240.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14696..15598
                     /locus_tag="OO369_003058"
     CDS             14696..15598
                     /locus_tag="OO369_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5983241.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15745..15944
                     /locus_tag="OO369_003059"
                     /pseudo
     CDS             15745..15944
                     /locus_tag="OO369_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         3956 a         4126 c         3857 g         4005 t
ORIGIN      
        1 gatacgcagc ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg
       61 caacattgtt ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg
      121 aatcaaaata catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa
      181 atcacttaaa cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa
      241 ataaataaaa aaccactaac tctatgtgaa ataaatcaaa acttcacgcc gaaatactcc
      301 ttaggatgta tagcgaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt
      361 gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac
      421 atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg
      481 ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt
      541 tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg
      601 tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt gaatccgttc gcggtgaagg
      661 actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca
      721 tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac
      781 gctgacctgc gtacccggcg tgatggtgct gatggaaaac agcgccacgc gactgaaacg
      841 ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct gcattgtgat
      901 gaacgccaat ccctttacga atggtcaccg ttatctgatt caacaagctg cggcacagtg
      961 cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg
     1021 gctggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc
     1081 agaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac agagcgtcat
     1141 taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg ctcccgcgct
     1201 tggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa
     1261 ccaggatatg cgctactggc tggaaacgcc gactatctcc gcagcgccca tcgaactggt
     1321 tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact
     1381 gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta
     1441 tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agacccccgc
     1501 atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct
     1561 ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat
     1621 agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc
     1681 cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact ggactgcatt
     1741 ttacatgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg
     1801 gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca
     1861 tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg
     1921 atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac accgcccgcc
     1981 gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg
     2041 tcaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt cgcggtggtg
     2101 cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat attgaaaaag
     2161 agatcctgcg tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg
     2221 cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct tccgagcgtt
     2281 tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc
     2341 cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg
     2401 gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag
     2461 aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga
     2521 ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccaccgcg
     2581 tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca
     2641 agatggtgga cggtccggtt atcgatcgcg cccgtctggt gctctcccgt gcagaacttt
     2701 ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga
     2761 caagaacggg tagcggcctg gaatcgtcgc actgaatgcg atcttgccgc tttccagaac
     2821 tcaccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg
     2881 gaagaagcga ttcgtcgctc aggtttacag gacggcatga cggtttcctt ccatcacgct
     2941 ttccgtggcg gtgacctgac cgtcaatatg gtgatggacg tcatcgcgaa gatgggcttt
     3001 aaaaacctga ccctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac
     3061 attcgccagg gcgtggttac ccgcatttat acctctggcc tacgcggtcc actggcggaa
     3121 gagatctccc gtggtctgct ggcggaaccg gtgcagatcc actctcacgg cggtcgtgtg
     3181 catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgt
     3241 gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat
     3301 gcaatggttg atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga actgctgcct
     3361 tatccgcata atccggcaag cattgagcaa gatcaggttg atttgatcgt caaagttaat
     3421 cgcgttggcg atgctgcaaa aatcggcgct ggtactaccc gtatgaccac taacccgcgc
     3481 gaactgctta ttgcccgtag cgctgcggat gtgattgtca actctggcta cttcaaagaa
     3541 ggtttctcca tgcaaaccgg caccggcggc gcttcgctgg cggtaacccg tttcctggaa
     3601 gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggcggtat taccgcgacg
     3661 atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca gagctttgac
     3721 agccatgctg cgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag
     3781 tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt ggtactgagc
     3841 gcgctggaaa ttgacaggca gttcaacgtt aacgtgctga ccggttctga cggcgtactg
     3901 cgtggcgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc
     3961 gcgccactgg tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc
     4021 ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt
     4081 ccggaactag cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg
     4141 cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc
     4201 gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa
     4261 taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg
     4321 ctcgtcagcc gggatgaaag gcaagcacgg caacacgcct ggctcaagca ccatcctgtt
     4381 ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagcga ggtcacacgc
     4441 cgaattttta atcatcgcgt gacagccttg cgtgccttag ccacaaagca gggctggcaa
     4501 attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt gagcattgcc
     4561 gccccggctc gcgacctcaa gctcgccacc tcaagctcgc caccattgag cttgaacata
     4621 gtcatcctct cgggcggtta tgggatatcg atgtcctgac gcccgaaggc gaaattctct
     4681 cccgccgcga ctattcactg cgcctcgccg ctgcctgttg tgcgagcaaa gcgcagccgt
     4741 ctgcgcgcgt ggaaaaaccc atcaactgac cgatttactc aaccgcatgg aggcactgct
     4801 gaacgatgtc gatgcctgca acgtcaacta aaaccacaaa gcttgcgacg tcattaatcg
     4861 atgagtacgc cctgctgggc tggcgcgcca tgctgactga agtcaatctg tcaccgaaac
     4921 caggcctcgt ggatcgcatt aactgcggtg cgcacaaaga tatggcgctg gaagatttcc
     4981 accgcagcgc gctggcgatt cagggctggt taccccgttt cattgaattt ggtgcctgta
     5041 gtgcagaaat ggcaccggaa gcggtactcc acggattacg cccaattggt atggcttgcg
     5101 aaggtgatat gttccgcgcc actgcgggcg taaacacgca taaaggcagc attttttctt
     5161 tagggctgct atgtgcggca attggccgtt tgcttcagct caaccagccg gttacgccaa
     5221 caaccgtttg ttctacggcg gcaagtttct gccgtggcct gaccgatcgc gaactgcgta
     5281 ccaataattc acgactgacg gcaggtcaac ggttgtacca gcagcttggc cttaccggcg
     5341 cacgcggtga agccgaagca ggttatccac tggtgatcaa ccacgccttg ccgcattacc
     5401 tcactctgct ggatcagggg ttagatcctg aactggcatt gctcgatacc ttgctcctac
     5461 tgatggctac caacggcgat accaacgttg catcgcgcgg tggcgagggg ggcctgcgct
     5521 ggctacagcg cgaggcgcaa acattattgc aaaaaggggg cattcgaacc cccaccgatc
     5581 tcgattatct ccggcagttc gacagggagt gtatcgaacg aaatctcagt ccaggcggca
     5641 gtgccgacct gctgatcctt acctgttttt tagcacagat ttaattattt aagcacttga
     5701 taaatttgga aatattaatt ttcggagaac ccgtatgtct ttagcaaaag ataatatatg
     5761 gaaactattg gccccactgg tggtgatggg tgtcatgttt cttatccctg tccccgacgg
     5821 tatgccgccg caggcatggc attacttcgc tgtgtttgtg gcaatgattg tcggcatgat
     5881 cctcgagcca attccggcaa cagcgatcag ttttattgcg gttactattt gcgttattgg
     5941 cagtaattac ctgctctttg atgccaaaga attagctgac ccagcgttta atgcgcaaaa
     6001 acaggcgctg aaatggggcc tggctggttt ttccagcacc acggtatggc tggtatttgg
     6061 cgcatttatt tttgcattag ggtatgaagt ttccgggtta ggtcgtcgca ttgccctttt
     6121 cctggtgaaa ttcatggaca aacgcacgct gacgttgggt tatgcgattg tcattatcga
     6181 cattctgctg gcaccgttta caccgtccaa caccgcgcgt accgggggta cggtttttcc
     6241 ggtcattaaa aacctgccgc cgttgtttaa atcattcccg aacgatccgt ccgcgcgtcg
     6301 tattggcggc tatttgatgt ggatgatggt cattagtacc agtctgagtt cgtccatgtt
     6361 tgtcaccggt gcggaccaaa cgtgctgggt ctggagttcg tcagcaaaat tgccggtatc
     6421 cagattagct ggttgcagtg gttcctctgc ttcctgccgg ttggggttat cttgcttatc
     6481 attgcgccgt ggctttccta cgtgctgtac aaaccggaaa tcacacacag tgaagaagtg
     6541 gcaacctggg cgggtgatga actaaaaacc atgggtgcgc tgacacgcag agagtggacg
     6601 ctgattggcc ttgtattgct cagcttaggt ttgtgggtat ttggcagtga agtcattaat
     6661 gctactgcgg ttggtctgct ggcagtttcg ctaatgctgg ctctgcacgt tgtgccgtgg
     6721 aaagacatta cccgctataa cagcgcatgg aacacgctgg tcaacctggc aactctggtt
     6781 gtgatggcta acggcctgac tcgttctggt tttattgact ggttcgccgg taccatgagt
     6841 acgcacctgg aaggattctc accaaacgca acggtgattg tactggttct ggtgttctac
     6901 tttgcacact acctgtttgc cagcctgtct gcgcacaccg caaccatgct gccggttatt
     6961 ctggccgtcg gtaaaggtat tccgggcgta ccaatggaac aactgtgtat cctgctggtg
     7021 ctgtctatcg gtatcatggg ctgtctgacg ccgtatgcaa ccggtcctgg ggtgattatt
     7081 tacggctgtg gctatgtgaa atcaaaagat tactggcgtc ttggcgcaat cttcggggtg
     7141 atttacatct ctatgttgct gttggttggc tggccgattc tcgccatgtg gaactaatac
     7201 gttcgccaaa cgctgaataa atcatcacgc ccgccaggta agcccacctg gcgggctttt
     7261 ttatgattta atagatagta ttcagtgtat gtatgagttc cacacccatt atgaaagcat
     7321 tctgccgtaa cgccgcgttg ctcgcggttt ctctgcttcc cttatcttct gccaacgccg
     7381 tagcgttgca ggcaaaacag tatggcgatt ttgatcgcta tgtcctggcc ctctcctggc
     7441 aaaccggatt ttgccagagt caacacgatc gaaatcgtaa cgaacgagat gaatgtcgcc
     7501 tgcaaaccga aacgaccaac aaaactgatt ttctgaccgt acatggtctg tggccaggat
     7561 tgcctaaatc ggttgctgcc cgtggtgttg atgaacgccg ctggatgcgc ttcggttgcg
     7621 ctactcgccc aatcccgaat ctaccagaag cacgcgccag ccgaatgtgt tcatcgccgg
     7681 aaaccgggtt atcactggaa acggccgcta aactaagtga agtcatgcca ggagctggcg
     7741 gacgttcctg cctggaacgc tacgaatatg ccaaacacgg tgcctgcttt ggttttgatc
     7801 cggacgcata cttcggtacg atggtacgcc tgaatcaaga aattaaagag agcgaagccg
     7861 gaaaattcct tgcggataat tacggtaaaa cagtgagccg ccgtgacttt gacgccgcct
     7921 ttgccaaaag ctggggaaaa gagaacgtga aagcagttaa gctaacgtgc cagggtaacc
     7981 ctgcgtattt gactgaaatt cagatctcga tcaaagctga cgccatcaac gctccgcttt
     8041 ctgcaaactc attcttgcca caacctcacc caggtaactg tggcaaaacc tttgtgattg
     8101 ataaagcggg ttattaatcc tctgatttac aaaacacgct aattcgtctt ccataaccat
     8161 gccgattaag aattatacat aatgcgctgc attgaggaat tccctgaatg tgacgcaaat
     8221 cacttcaaag agtgagcaac agtcagtatc atcatgtgag ttaaaccctc gccgcctgac
     8281 ggtgagggtt ttcttttggg tatgtttatc tgcgacactc gcagtaccga cgacatggag
     8341 taaaaatgtc cagaccaact atcatcatta acgacctgga tgccgaacgc atcgatattc
     8401 tgctggagca acccgcctat gctggtttgc caatcgccga cgcgttaaac gcagagttgg
     8461 atcgcgccca aatgtgttcg ccagaagaga tgccacacga cgtggtgaca atgaacagcc
     8521 gggttaaatt ccgcaatctt agcgatggcg aagtgcgtgt gcgcacgctg gtgtatccgg
     8581 caaaaatgac cgatagcaat actcagcttt ccgttatggc tccggtaggt gccgcactgc
     8641 tggggctgcg cgttggcgat tccattcact gggaacttcc gggcggcgtt gcaacccacc
     8701 ttgaagtgct ggaactcgaa taccagccag aagctgctgg cgactacctg ctttaatccc
     8761 atctgcacaa gcgataactc gctttgccca gaggatgcac tcgcatcctc tttacgcaaa
     8821 atcagcaatt tttcctggtt ctcactgttc acttttgctg caaaattatt ccgtttcgtt
     8881 tgtgggaata accatggcag aagcatttta catcctgata ggatttctga ttatggcggc
     8941 gattatcgtc atggctgtcc tttacctcga aaaccactcc tgacgatcat attgttcccc
     9001 ccggcatcgc attcaccaga cctgaaaccg ccttctgcgc cgcctctgcg ctttgtgcac
     9061 cgggtaccag aatcacctta cggcactcct cttcacgttt ttcgaaaatc tcatatcccc
     9121 gggcggcctc ttcaaacggc atatagtggg taacaatttc ttctggtttc agtaatcctt
     9181 tctcaattaa cggtaataat tctcccagcc atgcgtgaac gtgggtctgt cccattttaa
     9241 acgtcaaccc tttatcaaag gcgtcgccaa acaggaaacc gtgaataaat ccagcgtaga
     9301 cgcccggtac gctaacaatg ccgccacgcc tgaccgccgc aatacactgg cgcaacgctt
     9361 taccgctgct gccctccagt ttcaggttag tcagcaccgt ttccgtggtg ctgcctttcg
     9421 cttcaaaacc gacggcgtct attactgcat ccacgccccg gtgacctgcc gtttgttcaa
     9481 taattgactg tgccggatcg ctatcttcat cgaaattaat cgggatcgcg ccgtagcggt
     9541 cagcggcgaa acgcaagcgg tagggatgat gatcaacaac aaaaatctgt tccgcaccga
     9601 gcaaccgtgc acaggcgatt gtcaacaatc ccacaggacc agcaccatag actgcaacgc
     9661 ttgaaccttg ttggatctgc acattttttg ctgcctgcca tgccgttggc agaatatcag
     9721 aaaggaaaag ggctttatca tctgaaagca aaggcggtac tttaaacggc cccacattcc
     9781 ctttcgggac gcggacatat tccgcctgcc cgccaggaac gccaccatac aggtgactat
     9841 aaccaaacaa tgccgcggga gcaggtatct gttttttatt gagcgcagcg cctttacccg
     9901 cattggtatt ttcgcaggcg gcatattgct gtaatcgaca gaaaaaacag tcgccacaag
     9961 caatgacgaa cggaattacc acccggtcgc ctttttgcaa attttttacg tccttcccgg
    10021 tttcaacgac ttcccccata aattcatgac caaaaatatc gccatgttta acctggggta
    10081 ttttgcctcg ataaagatgg aggtcagagc cacagatcgc cgttaccgta atacgcagaa
    10141 taatatcatc tgcctgttca atgcccggat cgggaacatt ttctacctga acgtgatgtg
    10201 ggccgtgata agtcaatgct ttcatgcaac ctccagagac agttcaaaaa agagtcagtc
    10261 tttaagggta gctatcacag cgggaaaacg ggcggtaccg caggttttgg gagaattcct
    10321 agtgttcagt gagaatatat taatcacaga aaccgttcgt aaggaaaaag aaccagacaa
    10381 atatcaggga taaatattat aatcatggag aatgatttgt tcatgattaa cagggagaaa
    10441 gggttatgta taaggcaatc attatgccag ttgatgtatt tgaaatggaa ttgagcgaca
    10501 aagctgttcg ccacgctgaa ttcctcgccc aggatgacgg agttattcat ctacttcacg
    10561 tactacccgg gtcagccagc ctgagcctgc accgttttgc cgctgatgtg cgtcgttttg
    10621 aagagcatct gcaacatgaa gcagaagaac gtctgcaaac gatggtcagc cacttcacca
    10681 tcgatccttc ccgcattaaa caacatgtcc gttttggtag cgtgcgggat gaagtcaatg
    10741 agttggcaga agaactgggg gctgatgttg tagttatcgg ttctcgcaac ccatcgattt
    10801 cgacccatct gttaggttct aacgcctcga gcgtgatccg ccacgccaat ctgcctgtac
    10861 tggtcgttcg ctaacagata aaagtattta tgccgccctg cctggaacat cccggcaggg
    10921 ctttttaatg ccataaaaaa gccgccaggt ttgaccccgg cggctaagca attgcaggtg
    10981 aatcttactt cttcttatgc agttttggtg cgaatcaggt agtcaaaagc actcagagag
    11041 gctttggcac cttcgccagt ggcgatgatg atctgcttgt acggaaccgt cgtacagtca
    11101 cccgctgcga acacgccttt gacgttggtt tcacatttcg catcaatgat aatctcgccc
    11161 atgcggttac gttcgactgc gccttccagc cagttggtgt tcggcagcag accaatctgg
    11221 acaaaaatac cggccagttc gatgttgtga atattgccgc tgacacgatc tcgatattcc
    11281 agaccaacga ctttgctgcc gtcgcctttc acttccgtgg tttgcgcatt cagaataatg
    11341 tcgacgtttt tcaggctgcg cagtttgtcc tgcagaacct ggtcggcttt catttctggc
    11401 gcaaattcca gcagcgttac gtgctcaacg atacccgcca ggtcgattgc cgcttccacg
    11461 ccggagttac cgccgccgat aaccgctacg cgtttgcctt taaacagcgg gccgtcgcag
    11521 tgcgggcagt aggtcacgcc tttagtgcga tactgatctt cgcccggaac gttcatgttg
    11581 cgccattttg cacccgtcgc cacgataatg ctgcgtgctt tcagtaccgc gccagaagct
    11641 gtttcaatct ggtgtagacc accttcaacc gctgccggga tcagtttgct ggcgctctgg
    11701 ctgtcgatca catcaacgtc gtattcatca acgtgaactt tcagtgcgcc tgccaacttc
    11761 tgcccttcag tcttcggtac agaaatgtag ttttcgatat caacggtatc gaggatctga
    11821 ccaccaaaac gttcgcccat cagaccggta cggatgcctt tacgtgcgga gtaaattgct
    11881 gccgctgcac ccgccgggcc ggaaccaacg attaatacgt cataagcatc acgcttgttc
    11941 agctcttctg ccgcacgatt ttccgcgcca gtatcaattt tggcaacgat ttcagtcaac
    12001 gtcatgcggc cctgaccaaa ctctttcccg tttacgaaca ctgccggaac gcccatcacg
    12061 ttgcgatcgg tgatttcgtt ctggaaggtg ccgccgtcaa ttgcagtgtg cttgatgcgc
    12121 gggttcaata cgctcatcag gttcagcgcc tgcaccacgt ccgggcagtt atggcaagag
    12181 agcgagtaat aggtttcgaa ttcaaaatca ccgtcaatat ggcgaatctg ctccagcaga
    12241 gactgcgctt ctttcgacgg atgaccaccg gtccacagca acgccagtac cagcgaggtg
    12301 aactcgtggc ccagcgggga gcctgcaaaa cgtggtccct ggttggaacc tgggttggtg
    12361 atcaggaaag acggcttacg cactggcaag ctgttatctt ctttaaaggt gactttgtct
    12421 gacagttctg cgatttcagc caacagttcc ttgatttctg ccgatttagc gctgtcatcc
    12481 agcgtggcaa ttaactcaac aggcttggtc aatttctcaa ggtaagcctt gagttgagtt
    12541 ttcatatttg tgtcgagcat gtttatctcc tgggctttaa acatcatcat gcaagcggcc
    12601 tctgcggcta cctggatgca gcttgcatca tggtgcgggg aagaaaatcg ggtgccgcag
    12661 cacccgaaga attaggtgaa tttccgaagt atttcgcgtc agagcaaggc gactagcctg
    12721 tgaatccccg ggagcttaca caagtaagtg accggggtga gcgggcgacg ccaacgccgc
    12781 tatggcgtga aagacgaagg aaatttagat tttaccaacc aggtccagag acggagccag
    12841 agttgcttca ccttctttcc atttagccgg gcaaacttca cctgggtgag aagctacgta
    12901 ctgagctgct ttgattttac gcagcagatc agacgcgtca cggccaatgc cttcagcggt
    12961 aacttcgatt gcctggatga taccctgcgg gtcaacaacg aaggtcgcac ggtcagccag
    13021 accttcatct tcacgcatgt tgtcgaagtt acgggtcagg gcgccagtcg ggtcgccgat
    13081 catcgcatat ttgattttag cgatggtttc agagctgctg tgccatgctt tgtgggtgaa
    13141 gtgagtatcg gtagatactg cgtatacgtc tacgcccagt ttctgcagtt cttcgtagtg
    13201 gtcagcaacg tcacccagtt cggtcgggca tacgaaagta aagtcagccg ggtagaagaa
    13261 gaagacgctc cagcggcctt cggtatcttt ttcggtgatt tcgatgaatt cgccgttttt
    13321 gaatgcctgg tttttaaaag gtttaatttt ggtgttaatc aaggacatct atacttcctc
    13381 cgtgttttcg atgagatgta aggtaaccaa tttttgccga ttcggctaat gcgtttccat
    13441 tatcaaatcg ataagtttta ccttacaacc ttcgtaagac aactttgtga actttgcacc
    13501 gccagcggcg ataaaaagta aaatggcccc ctttccaggt gaccgtgtaa tctgaactac
    13561 cctttggtat cttcagcgcc tgcctgcagg ctgaggtgtt gcccttacaa atgcaacaac
    13621 gacatggatt acaacaccct cataaacaaa gggcaatcac ctgatctaag ctcttaccta
    13681 tgacagtgat aggttatgcc ttttactcga cttttgcact gactgaaaag aacaaattaa
    13741 tgttaaaaaa gatactttta ctggctctgc ttcctgcaat cgccttcgca gaggaacttc
    13801 cttccccagt aaaagcgatt gaaaaacagg gcattacaat catcaaaacg ttcgatgccc
    13861 ccggaggaat gaaaggttat ctcggaaagt atcaggatat gggcgtcacc atctacctga
    13921 ctcccgatgg taagcacgct atctctggtt acatgtacaa cgagaaaggt gaaaacctga
    13981 gtaacacact tatcgaaaaa gaaatttacg caccagccgg acgcgaaatg tggcaacgga
    14041 tggaacaatc ccactggctc ctcgacggta aaaaagatgc gccggtcatt gtctacgtct
    14101 tctccgatcc gttctgccca tattgtaaac agttctggca gcaggcgcgc ccgtgggtag
    14161 attctggcaa agtgcaatta agaacattgt tggttggggt tatcaagcca gaaagcccgg
    14221 cgacagcagc ggcaattctt gcctccaaag atcccgcaaa aacctggcaa caatatgaag
    14281 cctctggttg caagcttaag ctaaacgtgc ctgcaaacgt aagtacagag caaatgaaag
    14341 tgttaagtga caatgagaaa ctgatggacg atctgggggc aaatgtcacg ccggctatct
    14401 attacatgag taaggaaaat acgctgcaac aggccgtggg gttgcccgat cagaaaacgc
    14461 ttaatatcat tatgggaaat aaataaaagg taattcatgg atggttgaac tatatccctg
    14521 cttttcaacc atcctaaaaa agcatgaatt tctcgctcta ccctaagtgg ctgtgctatt
    14581 ttagaaaaac ttaatcctga gttaagcaaa aaaacataat caataaaaat acatacgcaa
    14641 aaaacattga ttaagtgaat ataccatgga agaaaaatat aaaccggagt agtgtatggc
    14701 caatctctac gacttgaaaa agttcgactt aaatcttctt gtcatatttg agtgtattta
    14761 ccagcattta agcattagca aagctgctga atcactatat atcaccccat cagccgtgag
    14821 ccagtcgtta caacgcttac gtgcgcaatt taatgaccct ttattcattc gctcaggaaa
    14881 aggtatcgcg ccgacgacaa cagggttgaa tctgcatcac catcttgaga aaaacctcaa
    14941 aggccttgaa caaacaatta atatagtcaa taagtccgaa ctaaagaaaa actttattat
    15001 ctacggccct cagctcattt cttgctctaa taatagtatg ctgatccgct gcctacggca
    15061 agatgcctct gtcgagattg agtgccatga tatcctcatg tcagcagaga atgctgaaga
    15121 actgttggtt caccgcaaag ccgatctggt gatcacccaa atgccagtga tcagccgttc
    15181 cgtcatttgc atgccattgc atacaattcg caatactcta atttgtagta ataagcatcc
    15241 gcgaattact gacaatagca cttatgaaca aattatggca gaagagttca cccaattaat
    15301 agccaaatca gcaggagttg atgatattca aatggaaata gacgagaagt ttatgaatcg
    15361 caaaatttca tttcgtggtt cctctttgct gacaataatt aatagcattg cagttacaga
    15421 tttactggga atagtcccct atgaacttta taactaccac cgtgactttc ttaatttaaa
    15481 agagataaaa ctagaacatc cgttaccttc aataaagctt tacatttcct ataataaatc
    15541 ttctttaaat aatttggtgt tctcccgatt tattgaccgt ctaaatgaga gtttttgata
    15601 tataactata gacagctact ataactttca tctatttatt cacagcgcga atgaaaaata
    15661 aaacactctg ctttacagtg tttactttat aattaacagt cttatctcac tgttcaccct
    15721 gtaacgttca gagagcgtct tcagatgtct atttataaaa ttcctcttcc gctcaatatt
    15781 ctggaagctg cacgagagcg tattacctag acactcaaca ccctaccccg cgtttgtgtc
    15841 tcattttctg gcggcaaaga ctctggttta atgctgcatc tgacagccga acttgcccga
    15901 caaatgggca aaaaaatctg cgttttgttt atcaactggg aggc
//
LOCUS       DAJTSA010000089        15526 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000089 DAJTSA010000000
VERSION     DAJTSA010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15526
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.089"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="OO369_003060"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="OO369_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR5983242.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="OO369_003061"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="OO369_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR5983243.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="OO369_003062"
                     /pseudo
     CDS             complement(941..1408)
                     /locus_tag="OO369_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO369_003063"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO369_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR5983244.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="OO369_003064"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="OO369_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR5983245.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="OO369_003065"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="OO369_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR5983246.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="OO369_003066"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="OO369_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR5983247.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="OO369_003067"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="OO369_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR5983248.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="OO369_003068"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="OO369_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR5983249.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="OO369_003069"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="OO369_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR5983250.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="OO369_003070"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="OO369_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR5983251.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="OO369_003071"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="OO369_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR5983252.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="OO369_003072"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="OO369_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR5983253.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO369_003073"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO369_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR5983254.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="OO369_003074"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="OO369_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR5983255.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="OO369_003075"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="OO369_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR5983256.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="OO369_003076"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="OO369_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR5983257.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="OO369_003077"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="OO369_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR5983258.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="OO369_003078"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="OO369_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR5983259.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="OO369_003079"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="OO369_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR5983260.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="OO369_003080"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="OO369_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR5983261.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="OO369_003081"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="OO369_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR5983262.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="OO369_003082"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="OO369_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR5983263.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="OO369_003083"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="OO369_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR5983264.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="OO369_003084"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="OO369_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR5983265.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="OO369_003085"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="OO369_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR5983266.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="OO369_003086"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="OO369_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR5983267.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="OO369_003087"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="OO369_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR5983268.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="OO369_003088"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="OO369_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR5983269.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="OO369_003089"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="OO369_003089"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR5983270.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="OO369_003090"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="OO369_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR5983271.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4007 a         3643 c         4130 g         3746 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgtgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 tgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1021 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggtaagaagg cccatgaatg ctaggcgcca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1261 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagag agcgctgatt tgttaacctg attccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatgc agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcagaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctattgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtaca ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcttgg gtattgtaaa
     5221 accatggaac tctacttggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcatt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggaaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattattgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttacaaa ggcaaggggg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactcta caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcttgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctatctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag atcactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtgtttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cttggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtacgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacttg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtt gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgctt ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca atgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agtatcccca cttatctaca atagctgtac tctttt
//
LOCUS       DAJTSA010000090        15448 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000090 DAJTSA010000000
VERSION     DAJTSA010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.090"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1393
                     /locus_tag="OO369_003091"
     CDS             <1..1393
                     /locus_tag="OO369_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5983272.1"
                     /translation="QYGFNNLLTLYGGTMVANNYYAFTLGTGWNTRIGAISVDATKSH
                     SKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYRR
                     DENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQL
                     SYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDVTTPRRQIYMSNSTTF
                     DDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQ
                     SSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGV
                     VVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKA
                     LRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITF
                     GKEIDESRNYICQ"
     gene            1409..2442
                     /locus_tag="OO369_003092"
                     /pseudo
     CDS             1409..2442
                     /locus_tag="OO369_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(2524..2862)
                     /gene="rcnB"
                     /locus_tag="OO369_003093"
     CDS             complement(2524..2862)
                     /gene="rcnB"
                     /locus_tag="OO369_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR5983273.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3081..3911)
                     /gene="rcnA"
                     /locus_tag="OO369_003094"
                     /pseudo
     CDS             complement(3081..3911)
                     /gene="rcnA"
                     /locus_tag="OO369_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            4033..4305
                     /gene="rcnR"
                     /locus_tag="OO369_003095"
     CDS             4033..4305
                     /gene="rcnR"
                     /locus_tag="OO369_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR5983274.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      4397..4493
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4528..5316
                     /gene="thiM"
                     /locus_tag="OO369_003096"
     CDS             4528..5316
                     /gene="thiM"
                     /locus_tag="OO369_003096"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR5983275.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            5313..6113
                     /gene="thiD"
                     /locus_tag="OO369_003097"
     CDS             5313..6113
                     /gene="thiD"
                     /locus_tag="OO369_003097"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR5983276.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6178..6999
                     /locus_tag="OO369_003098"
     CDS             6178..6999
                     /locus_tag="OO369_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR5983277.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            7047..7793
                     /locus_tag="OO369_003099"
     CDS             7047..7793
                     /locus_tag="OO369_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5983278.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(7767..8732)
                     /locus_tag="OO369_003100"
     CDS             complement(7767..8732)
                     /locus_tag="OO369_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCR5983279.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(8729..9732)
                     /locus_tag="OO369_003101"
                     /pseudo
     CDS             complement(8729..9732)
                     /locus_tag="OO369_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            9772..10824
                     /gene="fbaB"
                     /locus_tag="OO369_003102"
     CDS             9772..10824
                     /gene="fbaB"
                     /locus_tag="OO369_003102"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR5983280.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            11132..11986
                     /gene="gatY"
                     /locus_tag="OO369_003103"
     CDS             11132..11986
                     /gene="gatY"
                     /locus_tag="OO369_003103"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR5983281.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            12015..13277
                     /gene="gatZ"
                     /locus_tag="OO369_003104"
     CDS             12015..13277
                     /gene="gatZ"
                     /locus_tag="OO369_003104"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCR5983282.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            13287..13739
                     /gene="gatA"
                     /locus_tag="OO369_003105"
     CDS             13287..13739
                     /gene="gatA"
                     /locus_tag="OO369_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR5983283.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            13770..14054
                     /gene="gatB"
                     /locus_tag="OO369_003106"
     CDS             13770..14054
                     /gene="gatB"
                     /locus_tag="OO369_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR5983284.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            14058..15413
                     /locus_tag="OO369_003107"
     CDS             14058..15413
                     /locus_tag="OO369_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR5983285.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
BASE COUNT         3931 a         3648 c         3979 g         3890 t
ORIGIN      
        1 tcagtatggt tttaataact tattgacgct gtatggcggc acgatggttg ctaataatta
       61 ctatgcgttt accctcggaa cgggttggaa cacgcgcatt ggcgcaattt ccgtcgatgc
      121 cacgaagtcg catagtaaac aagacaacgg cgatgtgttt gacgggcaaa gttatcaaat
      181 tgcctacaac aaatttgtga gccaaacgtc gacgcgtttt ggtctggcgg cctggcgtta
      241 ttcgtcgcgt gattaccgga catttaacga tcacgtttgg gcaaacaata aagataatta
      301 tcgccgtgat gaaaacgata tctatgacat tgccgattat taccagaacg attttggccg
      361 taaaaatagc ttttctgcca atatgagcca gtcattgcca gaaggctggg gttctgtgtc
      421 cttaagtacg ttatggcgag attactgggg gcgtagcggc agcagtaagg attatcagtt
      481 gagttattcc aataactggc ggcggataag ctataccctc gcggcaagcc aggcgtatgg
      541 cgagaatcat catgaagaga aacgttttaa tatttttata tcaattccct gtgattgggg
      601 tgatgacgtt acgacgcctc gtcggcaaat atatatgtct aactcaacga cgtttgatga
      661 tcagggtttt gcctcaaata atacgggatt atcaggaaca gtagggagtc gggatcagtt
      721 caattatggt gtcaacctga gtcatcaaca tcagggaaat gaaacgacag ctggggcgaa
      781 tttgacctgg aacgcgccgg ttgcgacagt gaatggcagt tatagtcagt cgagtactta
      841 tcgacagact ggagccagtg tttcaggggg cattgtcgcc tggtcgggtg gcgttaatct
      901 ggcgaaccgt ctttccgaaa cgtttgctgt gatgaatgcg ccaggaatta aagatgctta
      961 tgtcaatggg caaaaatatc gcacaacaaa ccgtaatgga gtggtggtat acgacggaat
     1021 gacaccttat cgggaaaatc acctgatgct ggatgtgtcg caaagcgata gcgaagcaga
     1081 attacgtggc aaccggaaaa ttgccgcccc ttatcgcggc gcggttgtac tggttaattt
     1141 tgataccgat cagcgcaagc cctggtttat aaaagcgtta agagcggatg gtcaaccatt
     1201 aacgtttggt tatgaagtca atgatatcca tggtcataat attggcgttg tcggccaggg
     1261 aagccagtta tttattcgca ccaatgaaat accgccatcg gttaatgtag caattgataa
     1321 gcaacaagga ctttcatgca caatcacctt cggtaaagag attgatgaaa gtagaaatta
     1381 tatttgccag taatagtcag gtggttctat ggaaattcgc ataatgctat ttatattaat
     1441 gatgatggtt atgcctgtga gctatgcggc atgttatagt gagttatctg ttcagcacaa
     1501 tttggttgtt caggggaatt ttgcacttac tcaaacacaa atagcgacat atgagcataa
     1561 ttttaatgat tcgtcatgcg tgagtacaaa tactatcacc cctatgagcc catcggatat
     1621 tattgttgga ctttataacg ataccattaa attaaattta cattttgaat ggaccaataa
     1681 aaacaacatc acgttgtcaa ataatcagag cagtttcacc agtggttatt cggtaacggt
     1741 gacacctgcg gccagtaatg caaaagtgaa tatttctgcg gggggcggtg gttcagtgat
     1801 gattaatggt gttgcgacat tatccagtgc ttcatcatcg acacgcggga gtgccgcagt
     1861 acaatttcta ctgtgtttat taggtggcaa gtcatggggt gcatgtgtaa atagctacag
     1921 aaatgcattg gcacaaaatg caggtgtcta ttccttcaat ctgacattgt catacaaccc
     1981 gataaccaca acctgcaaac aggacgattt attaattact ttagacagta ttcccgtttt
     2041 acaattacca gccacaggta acaaagcaac aataaatagt aaaaaaaggg atattattct
     2101 gcgttgtaaa aatttattag gtcaacaaaa tcaaacatca cggaaaatgc aggtgtattt
     2161 atcaagttct gacttgttaa ccaacagcaa cacaatactg aaaggtgcgg aagataatgc
     2221 gtaggattta ttcttgaaag taatggttcg ccagtcacac ttttaaatat cactaacagc
     2281 agtaaaggat acacaaattt aaaagaaatt gcggcaaagt caaaacttac agatacaacg
     2341 gtttcaattc cgataacagc cagttactac gtctacgata caaacaaagt taaatctggc
     2401 gcactggagg caaccgcatt aatcaacgtg aaatacgact aataacaagc aaaacgagca
     2461 agtggctaat aataaaaaat aacaaggtgt ctgcatttcc ccctcatgat gagggggctt
     2521 tttttagcga tgataaaaaa tctcaccgtc gtaggcttta atgattttac cgtcggtgtc
     2581 gctgatcaac acgtacgcgc cacccatata ggtccagtgc gtcccggcat caggcgcggg
     2641 caggttacgt aactgccact gcttaatgtt gtactcatcg gtgcggtaca tctctgggac
     2701 ggtatcacct ggtttgaaat gcttaaagtc agcaatgaaa ctacttaact catacttaga
     2761 aattcccgag gtattggtcg aacccgcggt ggctggtgcg gcccaggcgg cactggtaac
     2821 cagcaaaagc acacccagca acatcttatt tttaatggtc ataaactctc cactttgggg
     2881 atctgtaata tcagtcgcat atctttcctg tcatacaata taactgacaa gcgatatttt
     2941 gttagtgatc ccatgatttc gtaaaaagtg taaatgaaag gatgccctaa aagcatcctt
     3001 agcgagcaga gagtcagcgg aaatagctga tttaagaatc atcctgggct gaagataaca
     3061 tgcaagccta aaggattgag ttatcgcatt atgcccatga agccatgtac gcccatatac
     3121 acaccgacta agccaatcaa cagactggaa aaataggggg cgcgtttagc gagagtgtta
     3181 aatccgctcc agcgttttgc gacctgctga acgctgattg ctgcgccaac gcctacggtg
     3241 acaagcgtta acgccaggcc aatgctgaaa ctgacgacca gtgttgcgcc cagtgtcagg
     3301 gctttcaact gaatgcatat caacagcacg gtaattgctg ccgggcaggg gataaagcca
     3361 ccggttaagc caaataacaa aatttgccag ttgatgacct ctctaccatc aaagcgtcgt
     3421 ttaatgtcat tggcatgggc tcgtgcatgg gcatcctgat actcgccatg ttcgtgatgg
     3481 tgatgatgtc cttggtcgtg gtgatgttcg tgatcgtgat gatggtgatg atgctcataa
     3541 tcatgcccgt gcatattctc cagccagttg cgttcgccgc gctaggtacg ccagaacatc
     3601 cagaacgcgg tgccaataat gatcactgcg gaaatcagct ggagccacgg ttctgctgat
     3661 tgagcagtaa agcgcttact gatcaccatc ccgccaaagg caatcaacca gaccaccgcg
     3721 gtatgcgaaa tggttgctgc cagtccgagc atcaccgcct gtttaatggt gcctttgatg
     3781 gcgatgataa acgccgccat catcgttttt gagtgccctg gttccaggcc atgaagcgca
     3841 ccaagtaaga tggcgctggg gatgaagaac caggcgtttc cttgctgaag aagagttgta
     3901 aattcggtca tgataataat tcttagtatt aattcggcaa tctgattcta ctccccccca
     3961 gtacctgata ctaccccccc agtagattaa tagtgctatg atttttcata ttcttgttaa
     4021 ccaggtgttg ccatgtctca tacaatccgt gataaacaga aactgaaagc gcgtgccagt
     4081 aagattcagg gccaggtcgt ggcgctcaag aaaatgctcg acgagccgca cgaatgcgct
     4141 gcagttttac aacagattgc tgctatccgt ggagcggtaa acggtctgat gcgggaagtg
     4201 attaaaggtc atctgacgga acacatcgtt caccaggggg atgagctaaa acgtgaagaa
     4261 gatctggatg tcgttctgaa ggtgctggat tcatatatca aataatttat taacgcgatt
     4321 gtaaaactgc cgtttttcct cgtttacaac gggggcgctg gacattacca tcctcctctg
     4381 cgatttatca tcgcaaccaa acgactcggg gtgcccttct gcgtgaaggc tgagaaatac
     4441 ccgtatcacc tgatctggat aatgccagcg tagggaagtc acggaccacc aggtcattgc
     4501 ttcttcacgt tatggcagga gcaaactatg caagtcgacc tgctgagttc agcgcaatct
     4561 gcgcacgcgt tacacctttt tcaccaacat tcccctcttg tgcactgcat gaccaatgat
     4621 gtggtgcaaa cctttaccgc caataccttg ctggcgctcg gtgcatcgcc agcgatggtt
     4681 atcgaaaccg aagaggccag tcagtttgcg gctatcgcca gtgccttgtt gattaacgtt
     4741 ggcacactga cgcagccacg cgcacaggcg atgcgtgctg ccgttgagca agcaaaaagc
     4801 tctcaaacac gctggacgct tgatccagta gcggttggtg cgctcgatta tcgccgccat
     4861 ttttgtcatg aacttttatc ttttaaaccg gcagcgatac gtggtaatgc ttcggaaatc
     4921 atggcattag ctggcattgc taatggcgga cggggagtgg ataccactga cgccgcagct
     4981 aacgcgatac ccgctgcaca aacactggca cgggaaactg gcgcaatcgt cgtggtcact
     5041 ggcgaggtgg attatgttac cgatggacat cgtatcattg gtattcacgg tggcgatccg
     5101 ttaatgacca aagtggtagg aactggctgt gcattatcgg cggttgtcgc tgcctgctgt
     5161 gcgttaccag gcgatacgct ggaaaatgtc gcatctgcct gtcactggat gaaacaagcc
     5221 ggagaacgcg cagtcgccag aagcgagggg ccaggcagtt ttgttccaca tttccttgat
     5281 gcgctctggc aattgacgca ggaggtgcag gcatgaaacg aattaacgct ctgacgattg
     5341 ccggtactga tccgagtggt ggtgcgggga ttcaggccga tcttaaaacc ttctcggcac
     5401 ttggcgctta tggttgctca gttattactg cactggtggc gcaaaatacc cgtggcgtac
     5461 agtcggtgta tcgcattgag cctgattttg tcgccgccca gctcgattcg gtgttcagcg
     5521 atgtgcgaat cgataccact aaaatcggta tgttggcgga aaccgatatt gttgaagcgg
     5581 tggcagaacg gttgcaacgt tatcagatcc aaaacgtggt actcgacacc gttatgctgg
     5641 caaaaagcgg cgacccgctg ctttcacctt cggcggttgc tacgctgcgc agtcgattat
     5701 tgccacaggt ttcattaata acgccaaact tgcccgaagc tgccgccttg ctcgacgcgc
     5761 cacacgcgcg caccgaacag gaaatgctgg aacaagggcg atcgctgttg gcgatgggct
     5821 gtggcgcagt gctaatgaaa ggtggtcatc tggatgatga gcaaagcccg gactggctgt
     5881 ttacccgcga gggtgaacaa cggtttaccg caccgcgcat tatgaccaaa aacacccacg
     5941 gcactggttg tacactctct gcggcgttgg ctgcactacg cccgcgccat acaaactggg
     6001 ctgacaccgt acaggaggca aaaatctggc tttcatcggc gttagcccag gccgacacgc
     6061 tggaagttgg tcacggtatt ggtccggttc accacttcca cgcctggtgg tgagcaccgt
     6121 actatagctg ctttggttat tgtcagcctt tggcactgac attcagggat gaaaccaatg
     6181 cagctaagaa ttaccagtcg aaaaaaactc accgctctat tatgcgctct tgggctaatt
     6241 tccatcgttg caatttatcc gcgtcagacg gtgaattttt tctattcgac agcagttcag
     6301 attacagact acatccactt ctacggttat cgcccggtta aatctttcgc tattcgcatt
     6361 cctgccagtt acaccattca cggattagat gtttcgcgct ggcaggagcg tatcgactgg
     6421 cagcgggtgg caaaaatgcg cgacaacgat atccgcttac agtttgcttt tattaaggct
     6481 acggaaggcg aaaagctggt ggacccctat ttttcgcgta actggcaatt aagccgcgaa
     6541 aatggcctgc tgcgcggggc gtatcattat ttctccccgt cggtatccgc ttcagttcag
     6601 gcgcgattat ttctgcaaac ggtggatttc tcacaaggcg atttccctgc cgtgctggac
     6661 gtagaagaac ggggaaaatt atcggcaaaa gaattacgca agcgggtaag tcagtggcta
     6721 aaaatggtcg aaaaacgcac gggaaaaaag ccgattattt actcaggagc cgttttttat
     6781 cacaccaatc tggcgggcta tttcaatgaa tatccgtggt gggtggctca ctattatcaa
     6841 cgtcgtccgg acaatgacgg catggcctgg cgcttctggc agcattccga ccgtggacag
     6901 gtagatggca tcaatggtcc ggtggatttt aatgtgttta atggcacggg gatgagttgc
     6961 aggcattcgt tgatgggatt aaagaaacgc cttaaataag aagcagtgat atactcccac
     7021 cagactccct ggcaggaaaa agacaaatgg aacaagcgca tacccagctg attgcccaac
     7081 tgaacgagcg tattttagcg gcggataaca cgccgcttta tattaagttt gccgaaacgg
     7141 taaaaaatgc cgtgcgcagc ggggtgctgg agcatggcaa tattttgccc ggtgagcgtg
     7201 atttaagtca gttaaccggc gtttcgcgca ttacggtgcg caaggcgatg caggcgctgg
     7261 aagaagaggg cgtggtgacg cgttcgcgcg gttacggcac gcagatcaac aacatcttcg
     7321 aatactcgtt gaaagaagcg agaggttttt ctcagcaggt ggtattgcgc gggaaaaagc
     7381 ccgatacgct atgggttaat aagcgtgtcg tgaaatgtcc cgaagaagtt gcgcagcagc
     7441 tggcggtcga agcgggaagt gatgtctttt tgcttaagcg tattcgctat gtcgatgaag
     7501 aagcggtatc gattgaggaa tcgtgggttc cggcgcattt aattcatgat gttgatgcca
     7561 tcggaatttc tctttatgac tatttccgca gccagcacat ttacccacaa cgtacgcgtt
     7621 cccgcgttag cgcccggatg ccggatgccg agtttcagtc acatattcag ttagatagca
     7681 aaataccggt gctggtgatc aagcaagtgg cgcttgacca acagcaacgg cctattgagt
     7741 acagcattag ccactgtcgc agcgatctat acgtttttgt gtgcgaggag tagttcctcg
     7801 cgcgttggcg cacaatcacc gccccgatgc ccgacaaccc acgacgccac tgcattgccc
     7861 agtaatacgg catccgccag tggcagacca gaggccagcc cggcaagtac gccacctgca
     7921 tgactgtcgc ccgccccaat ggtgtctaca acttgcgttg gaaatgccgg aatgcagcca
     7981 gaagcgtcgt tgctgaaata ccatgcgcct tctttatcga gacgaacgat caacggcgcg
     8041 gcaaatttct cctgccattg cttgccaagt gttgttatct ctgcggataa agcaaaacgt
     8101 tcggcggcaa tctcagcctc ttgacgattg agtgacacta aaggtcgaca ggccatgatc
     8161 cgcgccagta atgcgtccgg gatatcgcca atacgtgggc aaaaatcgat aaacggcgtc
     8221 acgtcttgca gcttttccag ccattccact aacaattcgc cgcagggcga ggccagttga
     8281 taaccggaaa aatagagcag gttgccaggc gcaacggtta atcgtgccaa ccattggcga
     8341 ttccattgat tttccacacc gctgaatgac ataaaagtgc gttcgccatc cggctcaacc
     8401 agtgccagac accagccgtt atcgctttcg gcgttatcga tcaggctgat taacccctct
     8461 ttcgccatcc ggttgcgaat aatctccgcc cacacgccct gaccgagcgg caaggcatta
     8521 cccgcttcga tgccgaggcg ctttaacgcc acggcaatat tcagtgcgca gccgccaacg
     8581 ttaacgctct gctgtttcag ttcgatatcg cacccacgcc aggggagggc ataagcgtcg
     8641 gcgatcacgt caatgaccgc cgcgccgacg accatcaccg gctgacgcgt ggttaattca
     8701 ggcagcagtg tgtgtaatcg agcgccgctc ataccgcctc ccgttgttga cgatatttcg
     8761 ccagcgctgt ggcatagcgg ttgaaatcaa gctgatttac cgcatccagc tccgccttta
     8821 acgcaggatc gacagcgtta atgccatgca acgcgccgca aatcgccgtc gccatagcac
     8881 ctatggtgtc tgtgtcgccg ccaagattgg cgcacaggac ggcgcagcgg tttgggtcgg
     8941 tttgtgccag ttcaactagc gcaatggcgc acggaacgga ctcaatagtg ctggtgcctg
     9001 cgccaacgac ctggtaaagc tgttcgctgg cggattcggt gccgtcggca ttacgcacaa
     9061 ttttcagcgc aatctccaga cgtgccgcca gtgaggcgct gaacgtggtg gtgcgttttt
     9121 gctgtgcatg tcgcgcaatt gaaggcaatg aatcaacaat cgctgaccag cgttctccgt
     9181 caatggcacg agaaatcgcc catgcgatga ctaccgcgcc tgcaaccgcc agatcggatt
     9241 tatgggtcgg gctggacgcc agcgccacat catcaataaa ggaatcaaca tcacgcgctg
     9301 gaagcaaaca acctaatggc gagacgcgca tcgctgcgcc gttggttacg ccgttgtttt
     9361 ccagttcagc aacgggttta ccgtcgcgaa tggcgttaag cgcaatcttc gaggtcggac
     9421 ctagtacgtt tttgttaaag gcgtcgaaac gcaacgccca gtcgagaata ttacgcccaa
     9481 tcagattcgg atcgatcttg ccttcacatt ccagtaacgc atccgccaga cacaggccat
     9541 cgaggtatcg tcggtgaatt cggcgcggtt aaaataacag gccgcgttat tctcctttgg
     9601 tccaggcaga aaacggtcaa tccagccaaa gtgtgcttta acgcggctgc gtggccaaag
     9661 ctcggagggc atccccatcg catcccctaa cgcctgccca taaagagcac cgagaatacg
     9721 ttctgttttc atttaacttc cccttgaatc ggaatttacg ggagcatagt aatgacagat
     9781 attgcgcagt tgcttggcaa agacgccgac aaccttttac agcaccgttg tatgactatt
     9841 ccttctgacc agctttatct ccccggacat gactacgtag accgcgtgat gattgacaat
     9901 aatcgcccgc cagcggtgtt acgtaatatg cagacgttgt acaacaccgg gcgtctggct
     9961 ggcacaggat atctttctat tctgccggtt gaccagggcg ttgagcactc tgccggagct
    10021 tcatttgctg ctaacccgct ctactttgac ccgaaaaaca ttgttgaact ggcgatcgaa
    10081 gcgggctgta actgtgtggc atcaacttac ggcgtgttgg tgtcggtatc gcggcgctat
    10141 gcgcatcgca ttccatttct cgtcaaactt aatcacaacg agacgctaag ttacccgaat
    10201 acctacgatc aaacgctgta tgccagcgtg gagcaggcct tcaacatggg cgcggtggcg
    10261 gttggtgcga ctatctattt tggctcggaa gagtcacgtc gccagattga agaaatttct
    10321 gcggcctttg aacgcgcgca cgagctgggc atggtgacag tgctgtgggc ctatttgcga
    10381 aactccgcct ttaagaaaga tggcgttgat taccatgttt ccgccgacct gactggtcag
    10441 gcgaaccatc tggcggcaac cataggtgca gatatcgtca aacaaaaaat ggcggaaaat
    10501 aacggcggct ataaagcgat taattacggt tataccgacg atcgcgtgta cagcaagtta
    10561 accagcgaaa acccgattga tctggtgcgt tatcagttag ctaactgcta tatgggccgg
    10621 gctgggttga taaactccgg cggtgctgca ggcggtgaaa ctgacctcag cgatgcagtg
    10681 cgtactgcgg ttatcaacaa acgcgcaggc ggaatggggc tgattcttgg acgtaaggcg
    10741 ttcaagaaat cgatggctga cggcgtgaaa ctgattaatg ccgtgcagga cgtttatctc
    10801 gatagcaaaa ttactattgc ctgatacgcc tcatacgaac agcaccaccc cgtaggacgg
    10861 ataagacgtt tacactgcat ccagcaacct atgcctgatg cgacgcggaa gcgtcttatc
    10921 atgcctacat agcactgcca cgtatgttta caccgcatcc ggcataaaaa cacgcgcact
    10981 ttgctacggc ttccctatcg ggaggccgtt tttttgcctt tcactcctcg aacaattttc
    11041 atattgttgt ttttgcgatc attatctcga catttaaaag caaataattt cattatattt
    11101 tgaaatcgaa agcaaacgac aagatatgaa aatgtacgtg gtatcgacaa agcagatgct
    11161 gaacaacgca cagcgcggcg gttatgcggt tccggcattc aatattcaca atctcgaaac
    11221 gatgcaagtg gtggtggaaa ccgctgccaa cctgcatgcg ccggtcatca tcgccggaac
    11281 gcctggcaca tttactcatg ctggtacaga aaatctgttg gcgctggtca gcgcgatggc
    11341 gaagcaatat caccatccac tggcaattca tctcgaccat cacacgaaat ttgacgatat
    11401 cgctcagaag gttcgttctg gcgtgcgctc agtcatgatt gacgcctcgc atttgccttt
    11461 tgcgcaaaat atttcacggg tcaaagaggt ggtggatttt tgccatcgct ttgatgtcag
    11521 cgtcgaggcg gagctggggc aacttggcgg ccaggaagat gatgtgcaag tcaatgaagt
    11581 cgatgcgttg tacaccaacc ccgcacaggc gcgtgaattt gccgaggcaa ccgggattga
    11641 ttccctggcg gtcgccatcg gcacggctca cggtatgtat gccagcgcac cggtgcttga
    11701 tttttctcga ctggagaaca ttcgccagtg ggtgaactta ccgctggtgc tgcatggcgc
    11761 gtcaggatta tcgactaagg atattcagca aaccatcaaa ctggggatat gcaaaatcaa
    11821 cgttgcaacg gagctgaaaa atgccttctt gcagtcgtta aaaaattacc tgaccgagca
    11881 ccccgaagcg accgatcccc gggattattt gcagtcggct aaatccgcaa tgcgcgatgt
    11941 ggtgagcaaa gtgattgccg attgtggctg cgagggcagg gcataacgca cctgccattt
    12001 aacaaggaaa aaacatgaaa acgttaattg cccggcataa agctggtgaa catatcggca
    12061 tatgttcagt ctgttctgcc catccgttgg ttatcgaagc ggcgctggca tttgatcgaa
    12121 acagcacgcg caaagtgctg attgaagcaa cgtcaaacca ggtcaatcaa tttggcggtt
    12181 ataccggaat gacaccggca gactttcgcg aatttgtttt tacgattgcc gataaagttg
    12241 ggtttgcacg cgaacgcatt attctcggcg gcgatcatct gggaccaaac tgctggcagc
    12301 aagaaaatgc ggatgcggcg atggaaaaat ccgtcgagct ggtaaaagca tatgttcgtg
    12361 ctggcttcag taaaattcat cttgatgcgt caatgtcctg cgcgggggat accataccgt
    12421 tagcaccaga aacggttgcg gaacgagctg ctgtgctttg ctttgctgcg gaaagtgtgg
    12481 cgacagattg ccagcgtgag caactgagct atgtcattgg cactgaagtt ccggttccgg
    12541 gcggtgaggc cagcgccatt cagtcagtac acatcaccca tgttgaagat gccgccaata
    12601 ctttacgtac gcatcaaaag gcctttattg cccgtgggct gacagaggcg ttaacacgtg
    12661 tgattgccat cgtggtgcag ccgggtgtgg aatttgatca cagcaatatt atccattatc
    12721 agccgcagga agcgcaggcg ctggcgcaat ggatagaaaa cacccgaatg gtttatgaag
    12781 cacattctac cgattaccag acccggacgg cttattggga attagtccgc gatcactttg
    12841 caatattgaa agtcggtccc gcattaacct ttgctttacg cgaggcgata tttgcactgg
    12901 cacaaattga gcaggaactt atcgcccccg aaaatcgcag cggttgcctg gcggtaattg
    12961 aagaagtgat gctcgacgaa ccgcaatact ggaaaaaata ttatcgcacg ggttttaacg
    13021 attcattact ggatattcgt tacagcctgt cggatcgtat tcgttattac tggccgcata
    13081 gtcggattaa aaatagcgtt gaaacgatga tggtgaatct gcaaggcgtg gacatcccac
    13141 tgggcatgat tagtcagtat cttcccaaac aatttgaacg cattcagtcc ggggaattat
    13201 cagcaatgcc gcatcagctt attatgaata aaatttatga tgttttgcgc gcctatcgct
    13261 acggctgtgc ggaataagga cggtatatga ctaacctgtt tgttcgtagc ggaatttctt
    13321 ttgtcgatcg tagcgaagtt ttaacctata tcggtaatga gatgctcgcg aaaggtgtgg
    13381 ttcatgatac ctggccacag gcattaattg ccagagaagc agaattccct accgggataa
    13441 tgcttgagca gcacgccatt gcaataccgc attgtgaggc gattcatgct aagtcgtcag
    13501 ccatttatct gttaaggcca acaaataaag ttcattttca gcaagcggat gatgataacg
    13561 acgtggcggt atcgttggtt attgcgttga ttgtggaaaa tccgcagcag caattgaaac
    13621 ttttacgctg tttatttggc aagttacaac agcccgatat cgtcgagaca ctaattactc
    13681 ttcctgaaac tcagttaaag gaatacttca caaagtatgt tttagattca gacgaataaa
    13741 tccctctgta acaataaaaa ggactattta tgaaacgcaa gattattgtc gcttgcggag
    13801 gcgcggttgc gacctctacg atggcggcgg aagaaattaa agagttgtgt cagagtcata
    13861 atattcctgt tgaattaatc cagtgtcggg ttaatgaaat agaaacctat atggatggtg
    13921 tgcatttgat atgtaccact gccagggtgg atcgtagttt tggcgatatt ccgttagttc
    13981 acggcatgcc ttttgtttct ggtgtcggta tcgaagcatt acaaaataaa attctgacta
    14041 tcttacaggg atgacctatg ttttcagaag tcatgcgtta tattctcgac ctcggcccta
    14101 cagtgatgct gccggttgtc atcattattt tttctaaaat attaggcatg aaggcaggcg
    14161 attgctttaa agcgggtctg catatcggga ttggctttgt tggcattggc cttgtgattg
    14221 gcttaatgct ggattccatt ggtccggcgg cgaaagcgat ggcggaaaat ttcgacctga
    14281 atctgcatgt ggtcgatgtc ggctggccgg gctcttcacc aatgacctgg gcgtcgcaaa
    14341 ttgcgctggt ggcgattccg attgcgatat tggttaacgt ggcgatgtta ctgacccgta
    14401 tgacgcgggt ggtaaatgtt gatatctgga atatctggca tatgaccttc accggcgcgt
    14461 tgctgcacct ggcaaccggt tcatggatga tagggatggc gggtgtggta attcacgcgg
    14521 cgtttgttta taagctcggc gactggtttg cccgcgatac ccgaaatttc tttgagctgg
    14581 aaggtattgc tattccgcac ggtacgtcgg cgtatatggg gccgattgcg gtgctggtcg
    14641 atgctatcat cgagaaaatc ccaggcgtta accgaattaa atttagcgcc gacgatattc
    14701 agcgcaaatt tggccctttt ggcgaacctg tcaccgtggg ttttgtgatg gggctgatta
    14761 tcggcatcct cgcgggttac gatgtcaaag gtgtattgca gctggcggta aaaacggcgg
    14821 cagttatgct gctgatgcca cggatgatta aacccatcat ggatggttta acgcccatcg
    14881 ctaagcaggc ccgtagtcgt ttacaggcga agttcggcgg tcaggagttc ctgattggcc
    14941 tggatcccgc attactgctg gggcatacgg cggtggtatc ggcaagcctg atttttattc
    15001 cgctcaccat tttaattgct gtttgtgttc cgggtaatca ggtactgccg tttggcgatc
    15061 ttgccactat cggcttcttc gtggcgatgg cggtcgcggt gcatcgtgga aatctgttcc
    15121 gcaccttaat ctcgggtgtc atcattatga gcatcaccct gtggatcgcg acgcaaacta
    15181 ttggtttgca cacccaaccg gcggctaatg ctggggcgtt aaaagccggg ggtatggtgg
    15241 cttcaatgga tcagggcggt tctcccatta cctggttact gattcaggtt ttctccccgc
    15301 aaaatattcc cggtttcatt attatcggcg caatttatct gaccggtatt ttcatgacct
    15361 ggcgtagagc gcgtggtttt actaaacaag agaaagccgt tctcgcagaa taatttttac
    15421 ctgaggggga ggttatcccc cttaacaa
//
LOCUS       DAJTSA010000091        15416 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000091 DAJTSA010000000
VERSION     DAJTSA010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.091"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1177)
                     /locus_tag="OO369_003108"
     CDS             complement(<1..1177)
                     /locus_tag="OO369_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5983286.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLM
                     "
     gene            complement(1376..1460)
                     /locus_tag="OO369_003109"
     tRNA            complement(1376..1460)
                     /locus_tag="OO369_003109"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1424..1426),aa:Leu,seq:caa)
     gene            1656..2679
                     /gene="ahr"
                     /locus_tag="OO369_003110"
                     /pseudo
     CDS             1656..2679
                     /gene="ahr"
                     /locus_tag="OO369_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
     gene            2808..4310
                     /locus_tag="OO369_003111"
     CDS             2808..4310
                     /locus_tag="OO369_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR5983287.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4429..5511)
                     /gene="lptG"
                     /locus_tag="OO369_003112"
     CDS             complement(4429..5511)
                     /gene="lptG"
                     /locus_tag="OO369_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5983288.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5511..6611)
                     /gene="lptF"
                     /locus_tag="OO369_003113"
     CDS             complement(5511..6611)
                     /gene="lptF"
                     /locus_tag="OO369_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5983289.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSHVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6878..8389
                     /gene="pepA"
                     /locus_tag="OO369_003114"
     CDS             6878..8389
                     /gene="pepA"
                     /locus_tag="OO369_003114"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR5983290.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8523..8966
                     /gene="holC"
                     /locus_tag="OO369_003115"
     CDS             8523..8966
                     /gene="holC"
                     /locus_tag="OO369_003115"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR5983291.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8966..11821
                     /gene="valS"
                     /locus_tag="OO369_003116"
     CDS             8966..11821
                     /gene="valS"
                     /locus_tag="OO369_003116"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR5983292.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11875..13071)
                     /locus_tag="OO369_003117"
     CDS             complement(11875..13071)
                     /locus_tag="OO369_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCR5983293.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13264..13767
                     /locus_tag="OO369_003118"
     CDS             13264..13767
                     /locus_tag="OO369_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5983294.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13813..14229)
                     /gene="rraB"
                     /locus_tag="OO369_003119"
     CDS             complement(13813..14229)
                     /gene="rraB"
                     /locus_tag="OO369_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR5983295.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14391..15404
                     /gene="argF"
                     /locus_tag="OO369_003120"
     CDS             14391..15404
                     /gene="argF"
                     /locus_tag="OO369_003120"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR5983296.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3979 a         3932 c         3972 g         3533 t
ORIGIN      
        1 acatcaacct acgctcccca agatgttccg ccttatggat gtaagctgct cgcacagagc
       61 tacgctcctg gtgactcatc tgccgctcta ccgcatccct cgaccataat cccgactcaa
      121 tcaacgaact acaagccatc gtcctgaagc catgcccaca aacctcgacc ttcgtgtcat
      181 accccattac gcgcaaagcc ttgttcaccg tattctcact catcggctta cgtggatcgt
      241 gatcgcccac aaaaatcagc tctcgatttc cgctcatgct tttgatcttt tccaaaatac
      301 ttagggcctg ccgcgacaaa ggtacaaggt gtggtgtccg catcttcgag ccacgctgag
      361 aatgcttgac tccttccagt tgctcacgct cacctgggat tgtccacata gccgtttcaa
      421 aatctatttc tgaccagcgg gcaaaacgca gctcgctcga acggatgaaa accaataaag
      481 tgagttcaac cgccagtcga gttaacggtc tgccagaata gtgatctatg cgatgaagta
      541 attcaggtat acggttcagt tcaagagctg cacggtgctg tcttttcgcc gtagcaaccg
      601 cacctgcaat ctcttgcgcg gggttgtagt cgattaagcc gctctgaacg gcaaagcgca
      661 taatcgcggt agtacgctgc tgtaaacggg cggcaacttc gagccgcccg gatgactcga
      721 ctgctttaat gggtacaaga agatcccgcg tcttcagttc cgcaatgttc cgcttaccga
      781 tagcagtaaa gagattatcc tccagacttt tcaaaacacg agcgctatgc gatgccgacc
      841 acttctgatt gctggcgtgc cagtctctgg cgactacttc aaacgttatc gcctcttgtt
      901 cctgttctac cttaacagct ttcttgtttt cactgggatc gacaccatta gctaaaagct
      961 tacgagcctc atcccggcgt gccctggcat ccgccaacga cacttcgggg tatttcccca
     1021 atgccagcat cttctctttg ccgccaaagc gataacgtag ccgccagtat ttggagccgt
     1081 tagggtgaac cagcaaaacc atgccttcac cgtcggtcag cttataggct tttgcttcag
     1141 gcttagccga acgaaccttc acatcactca gagccatgat gagtatcctt tcaagggttc
     1201 tgtgtgggta caaacattat cgaaccggga aatacccgca gttgtacccg catcagtaag
     1261 ttgatgtaga ttgaatcagg ttgacttagg ttgagtgaaa aagcgaggaa agccttgcgg
     1321 atactggatt ttaggcacaa aaaaagacgt ccgttgacgt ctattgatgt tccgatggtg
     1381 ccgaaggccg gactcgaacc ggcacgtatt tctacggttg attttgaatc aactgcgtct
     1441 accgatttcg ccacttcggc actgaagagg tatgcggaaa acgttgtgga ttatacctgt
     1501 cgcacgccac catgcaagcg ccagcttgca cccacctcgc taagtgctga aattttcagc
     1561 attacactca gcgatatgta acctttgtca cactccaggc accccgccct gccatgctct
     1621 acacttccca aacaacacca gagaaggccc caaaaatgtc gatgataaaa agctatgccg
     1681 caaaagaagc gggcggcgaa ctggaagttt atgagtacga tcccggtgag ctgaagccac
     1741 aagatgttga agtgcaggtg gattactgcg ggatttgtca ttccgatctg tcgatgatcg
     1801 ataacgaatg gggattttca caatatccgc tggttgccgg gcatgaggtg attgggcgcg
     1861 tagtggcact cgggagcgcc gcgcaggata aaggtttgca ggtcggtcag cgtgtcggga
     1921 ttggctggac ggcgcgtagc tgtggtcact gcgacgcctg tattagcggt aatcagatca
     1981 actgcgagca aggtgcggtg ccgacaatta tgaatcgcgg tggctttgcc gagaagttgc
     2041 gtgcggactg gcaatgggtg attccactgc cagaaaatat tgatatcgag tccgccgggc
     2101 cgctgttgtg cggcggtatc acggtcttta aaccactgtt gatgcaccat atcactgcta
     2161 ccagccgcgt tggggtaatt ggtattggcg ggctgggggg ggcatatcgc tataaaactt
     2221 ctgcacgcaa tgggatgcga ggtgacggcc tttagttcta atccggcgaa agagcaggaa
     2281 gtactggcga tgggtgccga taaagtggtg aatagccgcg atccgcaggc actgaaagcc
     2341 ctgtcggggc agtttgatct cattatcaat actgtgaacg tcagcctcga ctggcagcct
     2401 tattttgagg cgctgaccta tggcggtaat ttccatacgg tcggtgcggt tctcacgccg
     2461 ctgtctgttc cggcctttac gttaattgcg ggcgaccgca gcatctctgg ttctgctacc
     2521 ggcacgcctt atgagctgcg aaagctgatg cgctttgccg cccgcagcaa ggttgcgccg
     2581 acaaccgaac tgttcccgat gtcgaaaatt aacgacgcca ttcagcatgt acgcgatggt
     2641 aaagcgcgct accgagtagt cctgaaagcc gacttctgat ccctttgccg ggtgctgtcg
     2701 cccggcactt aaatctcttt ggaaggcgtc tcgcaatctg ctgaatttag cggtgaagag
     2761 tgcacagccg ttgcctatac tccaggcagg aaactggagg aaatctcatg agcgaacccc
     2821 tgttaattgc ccgcacgccg gacacagaac tgtttttact gccgggaatg gctaaccgtc
     2881 acgggctgat tactggcgca acggggacgg gtaaaaccgt tacgctgcaa aaactggcag
     2941 agtcattgtc ggaaatcggc gtgccggtgt ttatggctga tgtgaaaggc gatctgaccg
     3001 gtatcgcgca ggcaggaacg gcgtcggaaa aactgctcac aaggcttaaa aatatcggcg
     3061 tcaatgactg gcaaccgcat gccaatccgg tggtggtgtg ggatatcttt ggcgagaaag
     3121 gccacacggt gcgggcgacg gtttcggatc tggggccgct gttgctggcg cgactgttga
     3181 atctcaacga tgtgcaatct ggcgtgctga atattatctt ccgcattgcg gacgatcagg
     3241 gattgttgct gctcgacttt aaagatttgc gggcgattac ccagtacatc ggcgataacg
     3301 ccaaatcctt ccagaatcag tacggaaata tcagtagcgc atcggttggt gccatccagc
     3361 gcggattact gtcgctggaa cagcaaggcg cagcacactt ctttggtgag ccgatgctgg
     3421 atatcaaaga ctggatgcgc accgatgcca acggtaaagg cgttatcaat atcctcagcg
     3481 ccgagaaact ttatcagatg ccgaaactgt acgccgccag cctgctgtgg atgctttcag
     3541 agttgtatga acaattgccg gaagcgggcg atctggagaa accaaaactg gtgtttttct
     3601 tcgacgaggc acatctgctg tttaacgatg caccgcaggt actgctggat aagattgagc
     3661 aggtgataag gcttattcgc tcaaaaggcg tgggcgtctg gttcgtttcg caaaacccgt
     3721 ctgatattcc ggataatgtg ttcggccagc tcggtaatcg tgttcagcac gctttgcggg
     3781 cttttacgcc aaaagatcag aaagcagtga agacagcggc gcaaaccatg cgggccaatc
     3841 cggcatttga taccgaaaag gcaatccagg aactggggac cggcgaggcg ttaatctcgt
     3901 ttctcgatgc aaaaggaagt ccttctgtgg tggaacgggc gatggtgatc gcaccttgtt
     3961 cgcgaatggg gccggtgacg gaagatgagc gtaatggcct gattaatcac tccccggtgt
     4021 atggcaaata tgaagatgag gtggaccgcg agtccgccta tgagatgctg caaaaaggct
     4081 ttcaggccag taccgagcag caaaataatc cccccgtgaa agggaaagag gtggcggtgg
     4141 atgacggtat tcttggtgga ttgaaggata ttttgtttgg cactaccgga ccacgcggcg
     4201 ggaagaaaga tggtgtggtg caaacaatgg cgaaaagcgc cgctcgccag gtgacgaatc
     4261 agattgtacg cgggatgttg gggagtttgc tgggggggag aaaaagataa gtcagacgtg
     4321 atctgtgccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtt cgaactcgta
     4381 tttgactttg tcaacaataa taaagggagc tttcgctccc ttgttagatt acgattttct
     4441 cattaacagc cacaggctga ttaagaagaa gctggcactt ggcaacagtg cgccgatgat
     4501 cggcgggatg ccgtaaacca acgtcagcgg gccgaagatc tggtccagta cgtagaagac
     4561 aaaaccgaag ctgatacctg tgaccacacg cacgcccatc ggtacgctac gcagtgggcc
     4621 aaagataaac gacagcgcca tcagcatcat caccgccacg gatagcggct ggaagatttt
     4681 gctccacatg ttgagctgat aacgtccggc atcctgaccg ctcgacttca gatacttcac
     4741 gtagttgtgc aaaccactga tggagagtgc atccgggtcc agcgccacca cgcccagttt
     4801 gtctggcgtg aggtcggttt tccaggtgcc gctcaccgtc tgcgaaccgg taatctgttt
     4861 cggattggtc agatcagatt catcaacctg cgacagacgc cagactttat gttccgggtc
     4921 aaacttcgcg gtagcggcat aacgtacgga ttgcagacga cgattctcgt taaaggcata
     4981 aatactgatg ccacctaaca cctcgtcacc tttaacccgc tcaatgtaga cgaagttgtt
     5041 gccatctttc gcccataaac cttgctgggt agagagcaac gagccgccgt acatcgcctg
     5101 cgcacggtag ttacgcgcca tctgttcgcc ctgcggcgcg acccattcgc caatcgccat
     5161 cgtcagcaag accagtggaa tggcggtttt catcaccgac agcgctacct gcatacgggt
     5221 aaaaccagaa gcctgcatca ccaccagttc gctgcgctgc gccagcatcc caagaccaag
     5281 caacgcccca agaagagccg ccatcgggaa gaagatctgc acatctttcg gcacgctcag
     5341 caaggtatac attcctgcgc ctaacgcgtc gtaactcccc tgcccggctt ttttcagctg
     5401 atcgacaaat ttgataatgc ccgacagcga caccagcatg aacagcgtca tcatgatggt
     5461 ggtgaaaata gttttaccga tatagcggtc aagtacgcca aaaggttgca tcacaccgct
     5521 cctttacgcg aaaaactggc gcgcaggcgg cggaccggca cggtgtccca aaggttgaga
     5581 acaatcgcca aagccagata aatcaggtta acggtccaca tccacagtgt cgggtccagc
     5641 ttacctttac cgccgttcga tttcagggag gtctggatca ggaagaaaag tagatacagc
     5701 agcatggctg gcagcatcga cagtacgcgt ccctgacgcg ggttaaccac gctcaacggt
     5761 acaaccataa gtgccatcat aaacacggtg aataccaacg tgatacgcca gttcagttct
     5821 gcgcgagcac gatcggtgtc agtgttccac aatgtgcgca tgtccatctg gtcggtatcg
     5881 ttcgggtcga gcgccaccgc ctggtgacca atgatcgcct gataatcctg gaaatccgta
     5941 atgcggaaat cacgtaacag tgcagtgcct tcgaagcgcg ttccctggtt gagagtgacg
     6001 acatgggagc cgtcgcgcag ctgggttaaa tgtccggaat cggccaccac cacagaagga
     6061 cgtgcattac cttttggtcg aatttgtgcg aggaacacat ctttgaaatc gctgccgtca
     6121 acgctttcga tgaacagcac cgagctgcca ttagtcgctt gctggaattg cccttgcgcc
     6181 agcgccgcca tgccagggtt cgctttcgct tctgctaaca cttcatcctg atgacgcgat
     6241 gaccacggtc ccgcccacat cacgttaacc gccgcgacga ttgccgtgaa taccgcaagg
     6301 atcattgccg ctttcaccag aaccgctttg ctcaagccgc aggcatgcat taccgtaatt
     6361 tcactttcgg tatacagttt gcccagcgtc atcagcagcc cgaggaacag gcttaatggc
     6421 aggataagct gcgccatttc cggcacgccc aacccgagaa gggagagcac cagattcgcc
     6481 ggaatatcgc cgtcaaccgc tgcgccgagg atcctcacta acttttgaca gaagaagatc
     6541 aaaagcaaga tgaagagtat cgccagctgg cttttgagcg tctcccgcac cagatatctt
     6601 atgattatca ctttaaatac gcccgtaaaa actcgtcttt tgcaggattt tagcttgttt
     6661 catggcttaa acgtcattta ttctcttgag tcgtcgaaat cgtcgctaag ataattatac
     6721 tcaacggatt cacctctcag attttgttct gacgtgccaa tgccgtaata acgttaagat
     6781 taacacgaag tcatcgcaac agcggacatg agttacgaaa gcttgcaatt ctatctgtag
     6841 ccaccgccgt tgtctttaag attcaggagc gtagtgcatg gagtttagtg taaaaagcgg
     6901 tagcccggag aaacagcgga gtgcctgcat cgtcgtgggc gtcttcgaac cacgtcgcct
     6961 ttctccgatt gcagaacagc tcgataaaat cagcgatggg tacatcagcg ccctgctacg
     7021 ccggggcgaa ctggaaggaa aaccggggca gacattgttg ctgcaccatg ttccgaatgt
     7081 actttccgag cgaattctcc ttattggttg cggcaaagaa cgtgagctgg atgagcgtca
     7141 gtacaagcag gttattcaga aaaccattaa tacgctgaat gatactggct cgatggaagc
     7201 ggtctgcttt ctgactgaac tgcacgttaa aggccgtaac aactactgga aagtgcgtca
     7261 ggctgtcgag acagcaaaag agacgctcta cagtttcgat cagttgaaaa cgaataagag
     7321 cgaaccgcgt cgtccgctgc gtaaaatggt gttcaacgtg ccgacccgcc gtgaactgac
     7381 cagcggtgag cgcgcgatcc agcacggtca ggcgattgcc gccgggataa aagcagcgaa
     7441 agatctcggt aatatgccgc cgaatatctg taacgccgct tatctcgcct ctcaggcgcg
     7501 ccagctggct gacagctaca gcaagaatgt catcacccgc gttatcggcg aacagcagat
     7561 gaaagagctg ggaatgcact cttatctggc ggtcggtcag ggttcacaaa acgaatcgct
     7621 gatgtcggtg attgagtaca aaggcaacgc gtcggaagat gcacgcccaa tcgtgctggt
     7681 gggtaaaggt ttaaccttcg actccggcgg tatctccatc aagccttcag aaggcatgga
     7741 tgagatgaag tacgatatgt gcggcgcggc ggcagtttac ggcgtgatgc gtatggtcgc
     7801 ggagctgcaa ctgccgatta acgttattgg cgtgctggca ggctgtgaaa acatgcctgg
     7861 cgggcgtgcc tatcgtccgg gcgatgtgtt aaccaccatg tccggtcaaa ccgttgaagt
     7921 gctgaacacc gatgccgaag gccgcctggt actgtgcgac gtgttaactt acgttgaacg
     7981 ttttgagccg gaagcagtga ttgacgtggc gacgctgacc ggtgcctgcg tgatcgcgct
     8041 gggtcatcat attactggtc tgatgacgaa ccataatccg ctggcccatg aactgattgc
     8101 cgcgtctgaa caatccggtg accgcgcatg gcgcttaccg ctgggtgacg agtatcagga
     8161 acagctggag tccaattttg ccgatatggc gaacattggc ggtcgtcctg gtggggcgat
     8221 taccgcaggt tgcttcctgt cacgctttac ccgtaagtat aactgggcgc acctggatat
     8281 tgcaggaacc gcctggcgtt ctggtaaagc aaaaggcgca accggtcgtc cggtagcgtt
     8341 gctggcacag ttcctgttaa accgcgctgg gtttaacggc gaagagtaag tttgcgtttt
     8401 accctcaccc taaccctctc cctaagggag agggaaccga tcgagcgcaa tagaaattca
     8461 ttgctcatct ttcagggtga ggaatgcagc aatatttaaa tccaccacaa gaagccccat
     8521 atatgaaaaa cgcaacgttc taccttctgg acaatgacac caccgtcgat ggcttaagcg
     8581 ccgttgagca actggtgtgt gaaattgccg cagaacgttg gcgcagcggt aagcgcgtac
     8641 tcatcgcctg tgaagatgaa aagcaggctt accggctgga tgaagccctg tgggcgcgtc
     8701 cggcagaaag ctttgttccg cataatttag cgggagaagg accgcgcggc agtgcgccgg
     8761 tggagatcgc ctggccgcaa aaacgcagca gcagcccgcg cgatatattg attagtctgc
     8821 gaacaagctt tgcagatttt gccaccgctt tcacagaagt ggtagacttc gttccttatg
     8881 aagattctct gaaacaactg gcgcgcgaac gctataaagc ctaccgcgtg gctggtttca
     8941 acctgaatac ggcaacctgg aaataatgga aaagacatac aacccacaag atatcgaaca
     9001 gccgctttac gagcactggg aaaagcaggg ctactttaag cctaatggcg atgaaagcca
     9061 ggaaagtttc tgcatcatga tcccgccgcc gaacgtcacc ggcagtttgc atatgggtca
     9121 cgccttccag caaaccatca tggataccat gatccgctat cagcgcatgc agggcaaaaa
     9181 caccctgtgg caggtcggta ctgaccacgc cgggatcgct acccagatgg tcgttgagcg
     9241 caagattgcc gcagaagaag gtaaaacccg tcacgactac ggccgcgaag ctttcatcga
     9301 caaaatctgg gaatggaaag cggaatctgg cggcaccatt acccgtcaga tgcgccgtct
     9361 cggcaactcc gtcgactggg agcgtgaacg cttcaccatg gacgaaggcc tgtccaatgc
     9421 ggtgaaagaa gttttcgttc gtctgtataa agaagacctg atttaccgtg gcaagcgcct
     9481 ggtaaactgg gacccgaaac tgcgtaccgc tatttctgac ctggaagtgg aaaaccgcga
     9541 atcgaaaggt tcgatgtggc acatccgcta tccgctggct gacggcgcga aaaccgcaga
     9601 cggtaaagat tatctggtgg ttgcgaccac tcgcccggaa accctgctgg gcgataccgg
     9661 tgtagctgtt aacccggaag atccgcgtta caaagatctg attggcaaat atgtcattct
     9721 gccgctggtt aaccgtcgta ttccgatcgt tggcgacgaa cacgccgaca tggaaaaagg
     9781 caccggctgc gtgaagatca caccggcgca cgactttaac gactatgaag tcggtaaacg
     9841 tcacgccctg ccgatgatca acatcctgac ctttgacggt gatatccgtg aaagcgccca
     9901 ggtgttcgat accaaaggta acgaatctga cgtttattcc agcgagatcc cggcagagtt
     9961 ccagaaactc gagcgttttg ctgcacgtaa agcagtcgtt gctgcggttg atgcactcgg
    10021 cctgctggaa gaaattaaac cgcacgacct gaccgttcct tacggcgacc gtggcggcgt
    10081 ggttatcgaa ccgatgctga ccgaccagtg gtacgtgcgt gccgatgtac tggcgaaacc
    10141 ggcagttgaa gcggttgaga acggcgacat tcagttcgta ccgaagcagt acgaaaacat
    10201 gtacttctcc tggatgcgcg atattcagga ctggtgtatc tctcgtcagc tgtggtgggg
    10261 tcaccgtatc ccggcatggt atgacgaagc gggtaatgtt tatgttggcc gcaacgaaga
    10321 agaagtgcgc aaggaaaata acctcggtgc caacgttgcc ctgcgtcagg acgaagacgt
    10381 tctcgacacc tggttctctt ctgcactgtg gaccttctct acccttggct ggccggaaaa
    10441 taccgacgcc ctgcgtcagt tccacccaac cagcgtgatg gtatctggtt tcgacatcat
    10501 cttcttctgg attgcccgca tgatcatgat gaccatgcac ttcatcaaag atgaaaatgg
    10561 caaaccgcag gtgccgttcc acactgttta catgaccggt ctgattcgtg acgacgaagg
    10621 tcagaagatg tccaaatcca agggtaacgt tattgacccg ctggatatgg ttgacggtat
    10681 ttcgctgcca gaactgctgg aaaaacgcac cggcaatatg atgcagccgc agctggcgga
    10741 caaaatccgt aagcgcaccg agaaacagtt cccgaacggc attgagccgc acggcaccga
    10801 tgccctgcgc ttcaccctgg cggcgctggc gtctaccggt cgtgacatca actgggatat
    10861 gaagcgtctg gaaggctacc gtaacttctg taacaagctg tggaacgcca gccgctttgt
    10921 gctgatgaac acagaaggtc aggattgcgg cttcaacggc ggcgaaatga cgctatcgct
    10981 ggcggaccgc tggattctgg cggagttcaa ccagactatc aaagcgtacc gcgaagcgct
    11041 ggacagcttc cgcttcgata tcgccgcagg cattttgtat gagttcacct ggaaccagtt
    11101 ctgcgactgg tatctggagc tgaccaagcc ggtaatgaac ggtggaacag aagccgaact
    11161 gcgcggtact cgccatacgc tggtgactgt actggaaggt ctgctgcgcc tcgcgcatcc
    11221 gatcattccg ttcatcaccg aaaccatctg gcagcgtgtg aaagtacttt gcggtatcac
    11281 tgccgacacc atcatgctgc agccgttccc gcagtatgat gcatctcagg ttgatgaagc
    11341 cgcactggcc gacaccgagt ggctgaaaca ggcgatcgtt gctgtacgta acatccgtgc
    11401 agaaatgaac atcgcgccgg gcaaaccgct ggagctgctg ctgcgtggtt gcagcgcgga
    11461 tgcagaacgt cgcgtaaatg aaaaccgtgg cttcctgcaa acgctggcgc gtctggaaag
    11521 catcaccgtg ctgcctgccg atgacaaagg tccggtttcc gttaccaaga tcatcgacgg
    11581 cgcagagctg ctgatcccga tggctggcct catcaacaaa gaagatgagc tggcgcgcct
    11641 ggcgaaagaa gtggcgaaga tcgaaggtga aatcagccgc atcgagaaca agctggcaaa
    11701 tgaaggcttt gtcgcccgcg caccggaagc ggttatcgcg aaagagcgtg agaagctgga
    11761 aggttatgca gaagcgaaag ccaagctgat tgaacagcag gctgttatcg ccgcactgta
    11821 atcaacacag tgatgaaaac gaaggccgga gcatgctccg gcctttttta tctcttacag
    11881 aaatggtaaa gaaggcataa ctccccgaga aatactggct aagaagcctt tatctggttg
    11941 ttcttcttta ttgattaaag gaaggtcatc caaatcgccc agcaaatacg tattttttgc
    12001 ctgccagtca atcatccata ttttcagcag tgggtaagcc agaccacccg taatcccgga
    12061 tatcaccacc aacgcacaca tgcgataaag cataccgtgg tacgttaaag ttgagcgaaa
    12121 acgaatacgc ccatcattca gtgacaggtt gctcataaaa tgatttcgca acgacaccgt
    12181 taaataactg gtgctaacag caatcccaaa ataataaatt aactgcgcga ttatcatttt
    12241 acgttgcatt tccataaatt gctgtaaatt ctcaatatca tcatttgcat acccagaact
    12301 atcatactca tttaatattt gatcgaagat aatataacca gcaacagcga gaaaaggtaa
    12361 taaagctaaa aatgcgagga tggcatattt tatacaatac gcagtatcta atttcacctt
    12421 gaaacgatgt ataccgaaac tggtattgct ccagagaaaa ctcattacca ggctatataa
    12481 agtaccatta aaaataccaa tggaaacaat accaactatt gccatcagaa ccacagatat
    12541 aataacacta cttgaactat tggcatgtag catctttgtc gagataaaga aaacagtccc
    12601 catcccaatg gccattaaaa tcggcaagaa aaaggttacc caccagaacc ctttcaaaga
    12661 gcaattaaaa ctaaatctta cgccgttgag actggtcatt aaggcctgat aacgcagtcc
    12721 ttttgctgcc ataaatataa gcaaaaccaa cagtaacaaa gtcaaaacac agccaacaag
    12781 cggcatatct gctgacactg tcattaagat tgcgaaatag aaaaaaacaa aaacaagaca
    12841 actaacaaaa acattcccac cggtaattcc ataagaaaat cgttgtccgt taacttccat
    12901 attagcataa agataacgct tacatttcat taatgcccat ggtaaataga tacctagcgt
    12961 tataatcgtt aacaacacat tcaccacaca aataagaaaa tatctctcac ctgtaccatg
    13021 aaaaacaaaa gaatgggacg gaacatccat ttcattaata acttgagcca taaaaatccc
    13081 ctgaattaaa aaatccattg atataattta tagatacaga cagtaaaatg ttctgccacg
    13141 ttacaatact cttatcagta ataggctggc aaaaacttta ttgctttata agacgcttgc
    13201 gcacgtaaga agagagtggt attaaacaaa tgtataatta ctttattttt attgggttaa
    13261 actatgaata acattgcgcc gcaatcacct gtaatgcgtc gcctgacgct gcaggataat
    13321 cctgctatcg ccagcgtcat ccgtcaggtt tacgcagagt atggtcttac cgccgataaa
    13381 ggctacaccg ttgccgatcc gaatctcgac gaactgtatc aagtgtacag ccagcctggc
    13441 catgcttatt gggtggttga atacaatggc gaagtggtcg gcggcggcgg gattgcgcca
    13501 ttaacaggta gtgaagcgga catttgcgaa ctgcagaaaa tgtactttct tcccgctatt
    13561 cgcggtaaag ggctagcgaa aaaactggcc ttaatggcga tggagcaggc acgagagatg
    13621 ggcttcaaac gctgctatct ggaaacgacc gcttttttaa aggaagccat tgcgctttat
    13681 gagcatttgg gctttgagca tatcgactat gcgcttggct gcacgggcca tgtcgattgt
    13741 gaagtgcgga tgctacgcga actctaaaaa aatgcccgct agtatgtgct atcgggcatt
    13801 ttaatcaatt aattagtggc gaaccccgtc atcgtcttca tcgacaaaat cttcatcgtc
    13861 gccatcttcg ccgttaggat cttcaaagta agtgccccaa ccgtcgtact caacgtcaaa
    13921 tttctccgcc agcgtcatca gttgttcaac ctgggcatcg atcagatcgg cattcaacgc
    13981 gcactcgctg aggatgtcgc agcaaatcac gatatcacca tcttcaactt ccagctcttc
    14041 tggatcggtc acttcgtaac cgagtttaaa cgcttcaact gccgcttttt ccagagtttc
    14101 cagatcgtct gcggaaagat ggtgttcgat ggtgtacagc gcgtccggat cgctgccatc
    14161 ttccagtaat tcttcaataa tcaaacgtgt ttcttcacgc tgttcttcca gttgttccgg
    14221 gtttgccatg gctcattcct cttaatgtcc tgccgatact cttattgtca cacaccactg
    14281 acattgcctc cacctttgtg acaaagattt atgctttaga cttgcaaatg aataataata
    14341 cacataaatt gagttttaat tcattgaggc gttagccaca ggagggatct atgtccgggt
    14401 tttatcataa gcatttcctg aaattactcg atttcacgcc agctgaactc aacagcctgc
    14461 tgcagttagc cgcgaagctg aaagctgata agaaaagcgg taaagaagaa gccaaactca
    14521 ctggtaaaaa catcgcgctc atcttcgaaa aagactcgac tcgtacccga tgctctttcg
    14581 aagttgccgc atatgaccag ggggctcgcg ttacttatct tggcccaagc ggtagtcaga
    14641 ttggtcataa agagtcgatt aaagacactg cccgcgtgct tggtcgcatg tatgacggta
    14701 ttcagtatcg cggctatggt caggagattg tcgaaacact ggcggaatac gctggcgtgc
    14761 cggtatggaa cggcctgacc aatgagtttc accccacgca gttgctggcg gatctcctca
    14821 ccatgcagga gcatttaccc ggtaaagcat tcaacgaaat gacgctggtc tatgcaggtg
    14881 acgcacgtaa caacatgggc aattcgatgc tcgaagctgc ggcgcttacc ggtctggatt
    14941 tgcgtctggt cgcgccacag gcatgctggc cagaagctgc gctggttacg gaatgccgcg
    15001 ccctggcaca acaaaatggc gggaatatta cgctgacgga agatgtagct aaaggagttg
    15061 aaggtgctga ctttatctat accgatgtat gggtgtcgat gggtgaagca aaagaaaaat
    15121 gggctgaacg cattgcatta ctgcgtgatt atcaggtgaa cagcaagatg atgcagttga
    15181 ccggtaaccc ggaggtcaaa ttcctccatt gcctgcccgc gtttcatgac gaccaaacga
    15241 cgcttggcaa aaaaatggcg gaagagtttg gcctacatgg cggaatggaa gtgactgatg
    15301 aggtcttcga atctgccgcc agcattgtat ttgatcaggc ggaaaaccgc atgcatacca
    15361 tcaaagcggt gatggtcgcg acgctcagta aattaaataa ttaatatctc tataaa
//
LOCUS       DAJTSA010000092        15343 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000092 DAJTSA010000000
VERSION     DAJTSA010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.092"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(172..1197)
                     /gene="tdh"
                     /locus_tag="OO369_003121"
     CDS             complement(172..1197)
                     /gene="tdh"
                     /locus_tag="OO369_003121"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR5983297.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="OO369_003122"
     CDS             complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="OO369_003122"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR5983298.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="OO369_003123"
     CDS             complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="OO369_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR5983299.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3839..4771
                     /gene="rfaD"
                     /locus_tag="OO369_003124"
     CDS             3839..4771
                     /gene="rfaD"
                     /locus_tag="OO369_003124"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR5983300.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4781..5827
                     /gene="rfaF"
                     /locus_tag="OO369_003125"
     CDS             4781..5827
                     /gene="rfaF"
                     /locus_tag="OO369_003125"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR5983301.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5831..6823
                     /gene="rfaC"
                     /locus_tag="OO369_003126"
     CDS             5831..6823
                     /gene="rfaC"
                     /locus_tag="OO369_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR5983302.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6820..8028
                     /gene="rfaL"
                     /locus_tag="OO369_003127"
     CDS             6820..8028
                     /gene="rfaL"
                     /locus_tag="OO369_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR5983303.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8064..9206)
                     /locus_tag="OO369_003128"
     CDS             complement(8064..9206)
                     /locus_tag="OO369_003128"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCR5983304.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9215..10228)
                     /locus_tag="OO369_003129"
     CDS             complement(9215..10228)
                     /locus_tag="OO369_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCR5983305.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="OO369_003130"
     CDS             complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="OO369_003130"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR5983306.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="OO369_003131"
                     /gene_synonym="rfaI"
     CDS             complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="OO369_003131"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR5983307.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="OO369_003132"
     CDS             complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="OO369_003132"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR5983308.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12826..13950)
                     /locus_tag="OO369_003133"
     CDS             complement(12826..13950)
                     /locus_tag="OO369_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR5983309.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="OO369_003134"
     CDS             complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="OO369_003134"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR5983310.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         4380 a         3445 c         3145 g         4373 t
ORIGIN      
        1 tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca
       61 gctatataaa agatcagcgc aattacgaac tctttattac actgacagga ttttacataa
      121 ttaacagatt atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca
      181 gctcagaata actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc
      241 atcgatagag aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc
      301 cgccatcttg taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc
      361 tttaaagatc actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc
      421 aatacgaccg ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga
      481 catttccaga ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag
      541 gttttctttc gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg
      601 gtattcgtta acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc
      661 agccataata cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa
      721 cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga
      781 gatattgtcg gggattttga aagcgttgaa cgccggaatc accagatatt cggcgaagca
      841 gcccggacgg ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg
      901 acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt
      961 gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac
     1021 catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt
     1081 cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga gttccggtac
     1141 agggacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc
     1201 acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg
     1261 cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt
     1321 cggaacgacc ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc
     1381 aaatttctgc gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc
     1441 cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag
     1501 acggtcacgc aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac
     1561 aattgctggt gccagcgagt tggagaacag gtacggacga gaacgctggc gcagccactc
     1621 aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag
     1681 cgtgccggtg atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc
     1741 attttcaccg acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt
     1801 atctgccaga tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc
     1861 atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc
     1921 aagctcctgc atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc
     1981 atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag
     2041 cgtttcaaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat
     2101 ccccaggaat gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat
     2161 aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat
     2221 cagatccgga tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct
     2281 tccatcagcc acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa
     2341 caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg
     2401 cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg
     2461 acgagatgat gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc
     2521 tctcaacacc tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg
     2581 attttactgc attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca
     2641 gttggcatca aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc
     2701 tcagcgcctg catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc
     2761 attttcctct ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga
     2821 caaaaatgcc ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca
     2881 gaactaattt atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa
     2941 tatatacatg attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc
     3001 ctttttttat agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca
     3061 atcctcgagt tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg
     3121 ttttcactaa acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca
     3181 aaacatactc tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg
     3241 taaatgactc atctttttga attttttcga tacgacttct gatatatcta aatttctttt
     3301 ttatatcgat aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt
     3361 tcaaatcggg agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt
     3421 aactaaaata aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc
     3481 aatcccctct accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta
     3541 ataagaccat taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta
     3601 tattatccat tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac
     3661 tattcacatg caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt
     3721 tgcaattagc atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat
     3781 gattacagac attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat
     3841 gatcatcgtt accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga
     3901 taaaggcatc accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa
     3961 cctggtggat ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat
     4021 ggctggcgaa gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac
     4081 caccgagtgg gatggcaagt atatgatgga taacaactat caatactcca aagagctgct
     4141 gcactactgt ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg
     4201 cggacgcacc tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg
     4261 ttactcaaaa ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca
     4321 gattgttggc ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat
     4381 ggcgagcgtc gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt
     4441 cgaaggtagc gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa
     4501 cctgtggttc ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga
     4561 atccttccag gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata
     4621 cattccgttc ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac
     4681 aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga
     4741 atacatggcc tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg
     4801 cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg
     4861 ctatccccag gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg
     4921 gatgccggaa gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg
     4981 cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt
     5041 accaaactcc ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg
     5101 ctggcgcggc gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc
     5161 ctggccgcta atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac
     5221 cgcacaagat ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa
     5281 atcatatacc tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc
     5341 gggtgcggag tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa
     5401 gcagctgatt gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc
     5461 gggcaatgag attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc
     5521 gggggaaaca cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac
     5581 taacgattct ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta
     5641 tggtccaagt agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct
     5701 gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt
     5761 gatcgacatt actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga
     5821 agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac
     5881 gttgcccgca ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt
     5941 ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt
     6001 ggcaatacgt cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc
     6061 gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt
     6121 aaaaagcgcg gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca
     6181 aaccgctcgc gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca
     6241 gcagcacgcc gtagaacgca cccgcgaact gtttgccaaa agtttgggct atagcaaacc
     6301 gcaaacccag ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc
     6361 tggcgaatat gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga
     6421 acactggcga gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg
     6481 gggcgcgccg catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga
     6541 agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt
     6601 agtgtcggtg gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac
     6661 ggtttatgga ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg
     6721 tcgttctaca agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa
     6781 tcttgaaata ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc
     6841 tctctcgaga aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt
     6901 accacctatt ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca
     6961 ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca
     7021 tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc
     7081 cctgatgcag gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta
     7141 tgcacaatag caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc
     7201 gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat
     7261 tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct
     7321 gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa
     7381 taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta
     7441 tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa
     7501 caatggaagt tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc
     7561 acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat
     7621 cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt
     7681 ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat
     7741 ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt
     7801 ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag
     7861 tgtataaaaa atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc
     7921 ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat
     7981 ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag
     8041 gctgcataat gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt
     8101 tgagttacgc catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact
     8161 gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc
     8221 ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca
     8281 cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat
     8341 ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc
     8401 ttttcaggcg gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc
     8461 ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc
     8521 acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc
     8581 aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag
     8641 atattaagtt cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt
     8701 ccatttggta caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag
     8761 tataaactag ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta
     8821 tgcatatgta taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc
     8881 atgctattat gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc
     8941 aatattctct gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta
     9001 tatatgcgac tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt
     9061 gagtatcctg gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc
     9121 tgatacatcc acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta
     9181 accgtaaata ttattttatc aaccatctcg tatattaacc tttcataaca ttatatttaa
     9241 ttgctgtaaa aacaccatcg agaaattttt tctggtaaag caagtgttta tatttttctt
     9301 tgtgctcatg ttttttaaca gcttttttat aaggtatatt tctccatggt gatatattat
     9361 aaatattacg aaaataatct gcagaggcgt agttcgccca atcatgccat ggcttagtta
     9421 taccagtata atgtatgaag acagtgtcat cattaataaa ccgggtgtaa tattcactat
     9481 ttttttcttc aaattctgac tttatagtat aaatacaatt atattttctc ggtaatattt
     9541 tagcatgatg tagaaacatt aagtttaaaa tatcttgatc cgggtacttt aattttttta
     9601 taattgactc atctgataat agatcaaaga atttttccgt tagtctttgt tttaaccatt
     9661 cccgtaaatt aatatacatt acaccagagt taaaatagct accgttaaaa tcctcattac
     9721 acaaacgctc agcgctttta ctttgcatag catctacatc aattaccact gccccatatt
     9781 catctttaaa ctctaatgct attaactcgt tcaatgaacc cttacagacg atatcagcat
     9841 caagatataa taatgaatcc aatcgcgcag aaaaataatc aaatgaaagc aaacgataat
     9901 aaatagatac tggccagata tttgattgag gtagcgcctt caatggctca gaatcaatat
     9961 gatataattt aattactgtt tgatacttcg ttgctaattg tgataataat tcaatatact
    10021 ctttgtctaa gtaatcagcg attatgtgaa aggtaaattt gttttgttta ttcttttcaa
    10081 gaattgaagc aattgaaatc gcagccccaa cctggtagtt ctcatcaata ccccatgaga
    10141 cattaaacgt ttcccgttca tcaagtttaa cggggcgctt gtccagttca attatatctt
    10201 taaattgagt aagatgttta aaatccaatt taattaccct tcatcataat cattatttgc
    10261 tcttcaatcc tagtttgact tttacatccc tgattacttt tttaattttc tttttgaata
    10321 taagatatgt atagccaaag tctttcaatt cattttttat attatagtgc cgttcaagat
    10381 caatgcgatc ttttgctttt aaaacagcgg ttgttttttt acctgaaagg tcaattagtc
    10441 ttaaaccttt ttcggatact ataaaatttc ctttatgagg atcgccagac accattccat
    10501 gctgatgcaa ctcttttatc gattctgata attgatcctt cagatcttca gaaatttcca
    10561 aatactcatt taacccgaca ccttcaatat actcaatgag cataatataa taatgagcga
    10621 aatttaatgt tttacgttca gctagcaaaa aatagtcatt aattgattgg atgccttcac
    10681 cacgtacccg atcggtttga taaattaaat tttcataata atcttttttt atgcaagatt
    10741 ttaagaaacg ttcggtcatt ttatgctttg gcgcataaac tttaagtaca agaggtccac
    10801 gagcagtgtc aataagaatg actttagtat catcaataga acgaaaaact tttaatgttt
    10861 taaccctgca agttaaaaag tcatttagaa cttgctcata aaagggatca ttatctttta
    10921 tgaaaacttt caacccattt atcgttttat tatatatcat aatgaattat tttaacctta
    10981 aatctttaga agcatttttc tttataatac tttaaataat taaaaatgcc tgccatataa
    11041 tgcttttgtt taaatttatg ttttgcacaa taccgatact gattgctatt tacaggttta
    11101 agtaaatctt ctttactcca cggagaagct gctttggcga tcaaaaaact acgtgaaacc
    11161 ggataatcag cccactcatg ccagggtttt gtaggtccca catagtgtat aaacacggtg
    11221 tcatcattga ctggattatc aactttgtct tttaattcat agttaatact atatcgggta
    11281 ttatatttct ccgaaataaa cttcacttta ccattcaata atacattcag tacatcttga
    11341 tcaaggtggg tgattttact tacccaatct gggtcacgca acatttcaat agcttttgac
    11401 gaaatgttat ttagattcca ctcatcaata tttatcagta aaaaaccagc attgaaatat
    11461 ccagaagcta actgtggtgt agttaatacc gaggctcgat tttgccacca ttcaatatct
    11521 ctttcagcta caactgcggc aatttcatta gtagaaaatt gataatctaa gagttcttta
    11581 atactaccct tgcaagcaat atctgcatca agatatagta ttttttcatg tttatgatag
    11641 aaataatctg caattataaa tcgaaaatat gttgcgtaag tccagttttt cgtgcttggt
    11701 aatgacttca gcttatcaca attgataaca taaatattaa ttcttgaatt atattgtttt
    11761 gctaaatcag aaaaatataa tttgtcttta tcactgatat aatctgtgaa aacatgaaat
    11821 tcacaagaga tttctctatt gtttaataga atagatgcga tggctacacc acatccaaaa
    11881 agaaaatttc tgtcaatacc aaatgctata tcaagagtat ttttagatct tatattttga
    11941 taatgaaaat tatactcaaa aaggatgatg tcactatcat tgagttgaga catatttatt
    12001 ctgtatccaa aattttggca aaaataagtt acgggttaca acgattttcg aatcgttctt
    12061 tccctgattt ttgtggcttt tgcttctgct tgcgacagca gtccttgttc ctgcttgaga
    12121 atgtctttaa gcggggcggc aaaatacacc ttcataaagc gccagatatc ccgctgagtc
    12181 aggccaatat tcattgaaga aaaataaagc cctataagat ctttatcgcg ccaacgacct
    12241 ggaacgcgcg tgcgaagctg cgcccggtgt aggtcaatta ccgaaatttt taactcctct
    12301 tccttaccgg aaaaaggcaa gtgcagcagg aaatgacaga tataacagtc acgatggtta
    12361 atgcccgcag catgcatatc gcgcaccatc gtcgcgacac gcttaataag catacgcttt
    12421 acgcgaacat ctggtgggtt ggtcgcccag tcagcacagt aatcttccag actgatggtt
    12481 ggtgtcagat cttcggtaat aataaacgag gttctggtca gcggattcat gcctttttcg
    12541 ccgaatgcca ccccatacat agtatcaacg ccgacatccc gcagtcgatg aatcgcattc
    12601 cattcgcggt ctgcgcctaa tactggcatc cgcaatgaga gtaaattttt gattatctct
    12661 ttcagggtcg tgccgcgatg ccatttgaga aaatagcttt ttcccgccat ttcaaagcgc
    12721 agagtacggc gagtttccag ttcacgaaat acctcaccct gcaaggtttt aacttcctca
    12781 aaaggatctt tgccgcgcca taacgtggca aacggctctt taagttcaac catccagacc
    12841 acccgttatg atatccgccg ctttctctgg cagactgtat aaatcttgtg tatcagcata
    12901 atgtcgcgca ttttccgccc aagcctggcg caatgaagat tgcgttaacg ctttgcgtaa
    12961 aatctcattc aatgtttcct ggcggaatgg ctcagcaata gcctcgccgc aattagcgtc
    13021 gacaatataa tgcgcatagc cacaaacggc agttgttagt accggtaatc ctgcagtaat
    13081 cgcttccagc agcacaattc ccgccgcttc ctggtaggca ggatgcagta ataaatccgc
    13141 cgccgccatt aattccgaga catcgttgcg ccccgagaag aagtgaacat tactgcgcac
    13201 gccgcgtttt tctgccagtg cctcaaattt tcgcggttta tcctgcccaa caacatatag
    13261 caatgtgttg tggcgcagcg aatccggtaa cgaagcaagt gcttcaatgg aacgatcggc
    13321 acctttacgc gtgaagtctg aaccgacctg caacaataaa tattgttgtt cggttattcc
    13381 attcttctta cggaagattt cacggctatt ggctggctgc tggctatatt tacgatcagg
    13441 ataaatcccc ggtggcagaa tatgaaaacg ctccgcttca gtctgataat gtttctggaa
    13501 atcggcgatt tgcttatctg tcagcatcag cagctgtgtc ggcttgccct gttcgaaggt
    13561 tgcccgctca aaggcggcat aatggcgata acgtgacgtc aggcgataga aaaagccttt
    13621 ttcctgcgct actttctcgg cataacaaac atcagcggca taataaacgt ccagccccgg
    13681 cattttgttg aatccaacga ctttatcgac gggatgttcg cgtaaatgtt tttgcaccca
    13741 ggcaaaatac tccgcattgc gcccgtgatt ggtatgcgat ttaaccggca ctttgatcac
    13801 ttcaaataca tcagggcatt cgccttccca cgactgggta taaacccgaa catgatgacc
    13861 tcgggcggcg actgtctgag caatacgcat aaaatcgcgc tgcaaaccgc caaagggaaa
    13921 atatttatat aaacaaaaag caacgatcat aacgacgtac ctgtcgtgga ggagggcagt
    13981 aatttatcga cagcagcaat gacatctgcc gccggaataa ccgaaagata catctcattt
    14041 cggtcacgct gatcgcgcgt tggcatttcc cggtaatctc ccgcccagaa ttgaatcatg
    14101 ttatttgacc agggacgcca gaaaatatga tctgttgcgc caaacagcga tatcagcggc
    14161 gtattaactg cagcggcaat atgcgccggt gcggaatcaa cgccaataaa cagctgcgca
    14221 tgatcgatta acgcaccaag ttccgggaag gtcacctttc cagccagcgc cgttactggt
    14281 ggcgtctggc atccctgcgc aatttcattg acgcaggcca gatcgtcttt atctgggccg
    14341 gacgtcagaa cgacttcata accacgagca tgtaaggcat caatcacagc ggaaaacttg
    14401 gcgttgtccc agcatttgaa gatttgccgc gccgtaggtt ggataaccac ataattttgt
    14461 ccaacaccag cgtgatcaag ttcgcgacgc atacgtttcc agcttgcagg cgggtaactc
    14521 atggttgtct gcttcaccaa cgaatcaagt cccaatggtg tcagcacgga taagttactt
    14581 tccaccacat ttccaccctg caacggcacc aaatgggtga aacttttacg ccaaaaagca
    14641 gactgccgat gatgataatc ctgggaaatt ttcacacgag catttaataa gcgaaccagt
    14701 atagcaacca tccattgatc ggtaagattg acgataaggt catacttatt ggcacgtaat
    14761 accttgatga gatgaaaaaa gttggcaatt ttttctgagg cttttgcttt tttatttttt
    14821 atgccgtaga gcgcgttaat ctctggattt tcagacagga tcgggatggt gtcctgataa
    14881 agcagcacat cgatttttgc gtcagggtaa ttttttttca gagaactaat gacgggagta
    14941 gttaataaca tatccccatg aaaacgcatc ttaatgagca aaattcttcg aaatggctta
    15001 tccacaagta actctttttg ttgagattat ccgattaagg tgaagcagaa aaagcacgct
    15061 accgccccag gctcaacagc tacctgaata ctgataccgc actgatattg acgcgctagt
    15121 gtatcacttc tttcgcggca atccgacccg aatgattttt aagcacattg ttgattttca
    15181 ataaagaaat caatcaaata aaatcattag caaccatatt ctttcactgt tgctctgcga
    15241 ttccttcctg cacgtacagc agaaatcaat gaatcgcata aaaatacaaa aaaagtatag
    15301 atgttcaaca agaattggcg ggtaaattgc tatttaagtc aaa
//
LOCUS       DAJTSA010000093        14976 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000093 DAJTSA010000000
VERSION     DAJTSA010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14976
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.093"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(519..1460)
                     /gene="fabY"
                     /locus_tag="OO369_003135"
                     /gene_synonym="yiiD"
     CDS             complement(519..1460)
                     /gene="fabY"
                     /locus_tag="OO369_003135"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR5983311.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1505..1942)
                     /gene="dtd"
                     /locus_tag="OO369_003136"
     CDS             complement(1505..1942)
                     /gene="dtd"
                     /locus_tag="OO369_003136"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR5983312.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1939..2811)
                     /locus_tag="OO369_003137"
     CDS             complement(1939..2811)
                     /locus_tag="OO369_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR5983313.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2805..3404)
                     /gene="yihX"
                     /locus_tag="OO369_003138"
     CDS             complement(2805..3404)
                     /gene="yihX"
                     /locus_tag="OO369_003138"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR5983314.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3503..4288)
                     /locus_tag="OO369_003139"
     CDS             complement(3503..4288)
                     /locus_tag="OO369_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5983315.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4322..5218)
                     /gene="yihV"
                     /locus_tag="OO369_003140"
     CDS             complement(4322..5218)
                     /gene="yihV"
                     /locus_tag="OO369_003140"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCR5983316.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5386..6282
                     /gene="yihU"
                     /locus_tag="OO369_003141"
     CDS             5386..6282
                     /gene="yihU"
                     /locus_tag="OO369_003141"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR5983317.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6306..7184
                     /gene="yihT"
                     /locus_tag="OO369_003142"
     CDS             6306..7184
                     /gene="yihT"
                     /locus_tag="OO369_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR5983318.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7201..8442
                     /gene="yihS"
                     /locus_tag="OO369_003143"
     CDS             7201..8442
                     /gene="yihS"
                     /locus_tag="OO369_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR5983319.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8580..9482
                     /locus_tag="OO369_003144"
     CDS             8580..9482
                     /locus_tag="OO369_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCR5983320.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9681..11717
                     /gene="yihQ"
                     /locus_tag="OO369_003145"
     CDS             9681..11717
                     /gene="yihQ"
                     /locus_tag="OO369_003145"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCR5983321.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11763..13137
                     /locus_tag="OO369_003146"
                     /pseudo
     CDS             11763..13137
                     /locus_tag="OO369_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13180..14583
                     /locus_tag="OO369_003147"
     CDS             13180..14583
                     /locus_tag="OO369_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5983322.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14651..>14976
                     /gene="ompL"
                     /locus_tag="OO369_003148"
     CDS             14651..>14976
                     /gene="ompL"
                     /locus_tag="OO369_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCR5983323.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNKIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYL"
BASE COUNT         3674 a         3855 c         3735 g         3712 t
ORIGIN      
        1 gatttaatag cgtttgcttc ggtgatttat tactcattag ttgtttttct aaattaacaa
       61 cagaataact ttcttgttta atgagattaa tattggcaac cattatagcc tccttcccac
      121 ggtttaagag caaacataca acataatgat tcataacata cagagcagta aatgaatgat
      181 actgacaatg tatgccagcc cattatcttt gcataatatt tgtgagctat agattttaaa
      241 aatatttatg aaaaaagtat ggcaccagag ggggttttga ctatcaaaaa tcgctcaaac
      301 ctgaactaat atgatgagtt aagcattatc tgcaataatt gccactcagc tctacaaata
      361 atatttaaat gctcctgaac agtgatgtag caagacacgt atggtgccaa cgaagaataa
      421 aaatttataa agttcttatt caatataacc ggaatagtca gggactgatg taaatgaccg
      481 gaatgaatga gagaggcagc accgcccctc tccataagct actcttcttc gttcccgccc
      541 tcttcatacg ggccaaatgg cttcgcgggc agaacgatat acgtgccttc aaacactgca
      601 cccggcgtct cgtcgccaaa gatttcgacc tgcatctgca cccgtgcttt tcgtccgcgc
      661 gccagacggt cgagatcgcc gcttaaggca ccaaggtcgg ctaccgcatg gggtttaccg
      721 ctaatcggtt tgctgtagcg gatatgcgca tccgccagaa taatcgttcc gccgaggtgg
      781 cgttcacgca gcatcagcca gataagcccc caaccggtga gcgtcgccag cgagaataaa
      841 ctcccggcaa acagcgtatg gtgcggattc tgattgccgg tttccggcat ggtggtgata
      901 aatttttgcc cggtatattg ttgaatacgc acgcccattt tttcactaag cgggatatgt
      961 tcgtaccacg cctgttgcag ctgcgcgcac cagtcgccgc gatgcaaaat atcatccaga
     1021 gtggcgacgg gcttaatcat caaaaaatgg cgaatcggcg tggtggttgg cgtggtgatt
     1081 tctccctgat taacaaaccc cagcttggcg aaaaactcca ccgcgtcttc acgggcgcta
     1141 caggtcacgc gcttaacgcc ttcctgacgc gccaccgact ccagggtcat cgccatcagc
     1201 gttcctaacc ctttgtcctg cacgtccggg tgaacgacca taaagcgaat ggacgcttca
     1261 ttgtcggcat taatatacaa tcggcctacc gccaccagat taccctgctc gtcgacgacc
     1321 atctggtgat gcgccatcgc atcccacgcg tcgcgttccg aaccttttgg ttgatgcagg
     1381 ggcttacgca acatttccca gcgaaagtga tagtaacgct ctaactcttc ttctgtttgt
     1441 ggaacctgaa ggtgatacat agcgatactc tctcttgttt cccgtgacaa ccctggaagc
     1501 tggctcatac ctgcaaccag aatgtcacgg ggccatcatt gaccagcgat acctgcatat
     1561 cggcagcgaa gcgtcctgtt tgcgtgttca tctcttgctg gcggcagcgt tcgacgaaat
     1621 agtcatataa cgcctctgcg cgatccggtg atgcgccttt ggagaaactt gggcgcatcc
     1681 cccgttcggt atctgcggcg agggtaaact gggaaaccac cagtacactg ccgcccgcct
     1741 gttgcacgtt gagattcatc ttgccttcgg catcgctaaa gatgcggtag ccgagcacac
     1801 gctcgcacag acggtttgct ttctgttcgt cgtcatcctt ttcgacaccc aataacacca
     1861 aaagtcccgc gccaatttcg cccgtcactt ctccctccac ggtgacgctg gcacgggtta
     1921 cgcgttgaat taatgcaatc atggttcgtc gtcttcttct tgttcagctg cttgttttag
     1981 tttgcggtat tccccgagag tgacagtaat ttccgcgcca agcaagacga tacaccatgt
     2041 ccagtagacc caaacaaaga gaatggggat caccgccagc acaccgtaaa tgagctgata
     2101 tgacgggaac atggtgatat aaagcgcgaa acctttcttt cctgcttcga acaggagtgc
     2161 ggcgacaaac gcgccgacaa tcgcgtcgcg gttaggtacg cggatggtag gaacaatgct
     2221 gtacagcaac cagaaggaga tccacgacaa cagcaacgga aaaatacgca gcacgttgtc
     2281 gatgacagta ttgagatcgc tcgcccagcg cagggagagc aaataggaac tgatcgccag
     2341 actggcccct gccagcagcg gccccagcgt taaaatcatc cagtacacgg cgaacgagta
     2401 aatttttggt cgcgctcgtt tactgcgcca gatggtattc aacgcgctat cgatggagta
     2461 catcaacaat aacgcggtga cgatcagccc gcacgcccca acggcggtca tcttgttgga
     2521 attagcaaca aattgttcga tataccgctg aataacatcg ccagtagcag gcagaaagtt
     2581 ggcaaaaata aagtgacgta actgaatgct gacgtcggaa aacatgggaa aagcggcgaa
     2641 aagcgcaaaa acaacggcaa ccagcggcac taatgagagc aacgacacat aggcaaggtt
     2701 acctgccagg gttgtcatgt tgtcctcatc aatacgttgc cagagtagtt ttagccaggc
     2761 ccatagtgga cgggtacggt gcctggcttt gtcctgaatg gtttttagca taacaccttc
     2821 gcgaaatagt ccgggatggt ggttttatct ttcaccagaa tactggtaat gcctagctga
     2881 ttagctcctt ctatattatc ggcgttatcg tcgaaaaaga ccgtatcgct gggtgaaaaa
     2941 ccttccgcct gcaaaacatg ctggtaaatt cgtgcttcag gtttgcgcat ccccagatct
     3001 tgcgacagat agatatggtc agcagcatca cgaatttccg ggtattcttc cggccagaag
     3061 gtggtatgca ggcggttggt attggaaagc accaccacgc gatgcccctg ctcacgcagt
     3121 ttatgcatga tggcgatcac ttccgggcgc agcgcaacaa acaccgcctg ccagccgtga
     3181 gagaactgct cgtagcttag cggtagagcc atctcatgac acagcgcctc tgcgaacgct
     3241 tcgtcgctaa tttccccacg ctcatgctga tgaaacgcct cccccatatg aaaactcttc
     3301 ttaagcgatg ccagcggaat acgcgttaaa tcgctccagg ctcccagcac acggttaaag
     3361 tcgatatcga caatcacatt acctaaatca aagatataga gcatttttgc ctcctttcgc
     3421 gccacaagaa attaactgta gcgggaaagg atgggtttgg ctatgtggca aggtatgaag
     3481 ccaggttaac ggtcacaaaa atctacgcgt cttcctgggg aagaatgatt tcaatttggc
     3541 tgtcttgtaa gcgtgatttt agcgcagcgg gcggcagctc gtcggtaaag agcgcagtga
     3601 cctgtgccac gttaccaatt tcaaccgccg ccgaagcatg atacttagtg tgatcggcga
     3661 ccagcagaat atttctcgcg tgcgccatca tcgttttcac cacgttagct tcgtttacat
     3721 caaactccat caacgcgcca tcgctctcaa tcgccccaac gcttgttacc agataatcag
     3781 cacgaaaatc agccacaaag gacgccgctg aagggccaat gatcccgcta ttatgagagc
     3841 gcaacgtacc gccgggcacc atcacttcaa agcgcgggtt gtggtaaaga atatgcgcca
     3901 cacgcaggct gttggtgatt atccgcaaat gattatggtt aagtaacgcc cgggcaacat
     3961 gctcaacagt cgtaccaatg gtgataaata ttgttgaacc atcaggaata tagtctgcca
     4021 ccgcttcggc aatcgctttt tttttctcgg tttgcgaaac ctcacgctgc tcgaacgccg
     4081 tattaacgac gctggaagcc cgacccgcgc caccgtgatg gcgcgtaatc aggccttgct
     4141 cgcttaattt acgaatatcc cggcggaccg tctgcgtgga gacatccagc agatttgcca
     4201 gctcatcaat attcatatac ccacgctcgg cgatcagcat gaggagttgg tcgtgccgcg
     4261 ggttaccggt tagttcggta aggctcatga aaattcctcg aaaaaccatc accctggcat
     4321 tttatacaaa aagtgacaaa aaagatcggg tttgatcaca gtcagggatc ccggctcgtc
     4381 caccgggacg tgtgcatttt aacgccgcta caccgctggc gaagcggact gactccgcta
     4441 aatccccact tgttgccagc gccaccgcca aagcgccgtg aaaaacatct cccgcacctg
     4501 tggtatctac cacatcaact ttgaaggccg gttgatgctg acgcccacca ttttccagcc
     4561 agtcgcagcc tgcgctaccc tgggtcacat agacatgacc atttgtgagc gtttgtgcct
     4621 gttttagcgc actggccatc tctttcacgc ccgttaagcg cgccagaccc ggttctgaaa
     4681 aggccgcgtg atcgcttaat gccaccagct cactgatatc ctgcggcgta atgtccccgt
     4741 ccagaacggt catcacaccc gcctgacggg ccagggtgaa ggctttttta gcgccgtcgt
     4801 gccagcgtac atctgccagc acaacatccc actgagagaa atcaatttcc tccaaccact
     4861 ctgcgtcagg cagcagatcc gggctggggt agttaatgat tatccgctcg cctttggtat
     4921 ccaccatgat ggcggattgc gaagatttcg cctggttata ccgtttggtg taacgggtgt
     4981 taacgcccca ggattccagc tctgccagca ggctgttgcc cgcgtcgtca tcacctacgc
     5041 gaccaataaa atcgacctgc gcccccagcc ttgccgccgc aaccgctgcc gtcgccgctg
     5101 gcccgccacc aacttccgta taatttctcg ccacgtattt accgctctcc gtcggtaacc
     5161 cttccacgta atagatgcga tccatcacgg ttatacctac acaagcaaca cgaatcatgg
     5221 tcattcctta aacattttgg gatgcgttca ttttaatttc accaattgtt taaaaagtga
     5281 cggctgtcaa ttttttgacg ataaattaca aattcgatca aaaacagaca agcaaagcat
     5341 gtcataaatg acaaaaagtg acatcactgt attcaggaga gggttatggc agcaatcgcg
     5401 tttatcggtt taggacaaat gggttcgcca atggcgagca atttattgca gcaagggcat
     5461 caacttcgcg tctttgatgt gaacgccgaa gctgtgcggc atctggtaga caaaggtgcg
     5521 actcccgccg ccaacccggc gcaggccgct aaagatgccg aatttatcat taccatgttg
     5581 ccgaatggcg atctggtgcg caacgtgttg ttcggtgaaa acggcgtttg cgaaggctta
     5641 tctaccgatg cgctggtcat tgatatgtcc accatccatc cgctgcaaac cgataaattg
     5701 attgccgata tgcaagccaa aggcttcagc atgatggatg ttccggtagg ccgtacttct
     5761 gcaaatgcca ttaccggtac tctgttactg ctggctggcg gcaccgctga acaagttgaa
     5821 cgtgccacac cgatcctgat ggcgatgggc agtgagttga tcaacgctgg cggtccgggc
     5881 atggggatcc gcgttaagct catcaacaac tacatgagca tcgcgctcaa tgcgctttcg
     5941 gcagaagccg ccgttttgtg cgaagccctg aatcttccct tcgatgttgc cgtcaaagtg
     6001 atgagcggta ccgccgccgg taaaggtcac ttcaccactt cctggccaaa caaagtcctc
     6061 agcggagatc tttctcccgc cttcatgatc gatcttgccc ataaggatct tggcatcgcc
     6121 cttgatgtcg ccaaccagct gcatgtgcca atgccgctgg gggccgcctc acgggaggtt
     6181 tatagccagg cgcgcgcagc gggtcgcggt cgccaggact ggtccgccat tctggaacag
     6241 gtccgtgtca gtgccgggat gactgccaaa gtaaaaatgt aacgactgga taaaggaata
     6301 aatgaatgaa taagtacacc atcaacgaca ttacgcgcgc atcgggcggt tttgccatgc
     6361 tggcggtcga tcagcgcgaa gccatgcgca tgatgtttgc cgcggctggg gcacccgctc
     6421 cggtagccga tagcgtttta actgatttca aagttaacgc tgcaaaggcc ctctcgcctt
     6481 atgcctcggc gattctggta gatcaacaat tctgctaccg ccaggtggtt gagcaaaacg
     6541 cgattgccaa aagttgcgcc atgattgtcg ccgccgatga gttcattcct ggcaacggta
     6601 ttccggtcga tagcgtggtt attgaccgca aaatcaatcc gctacagatc aaacaagacg
     6661 gtggcaaagc cttaaaactg ctggtgctgt ggcgtagtga tgaagatgcg cagcaacgtc
     6721 tggatatggt gaaagagttc aacgaactgt gccactcaca cggtctggta agcatcattg
     6781 agccagtcgt ccgcccaccg cgtcgtggcg ataaattcga tcgcgaacaa gcgatcatcg
     6841 atgccgccaa agagctgggc gacagtggcg ctgacctcta caaagttgaa atgccgcttt
     6901 atggcaaagg tccgcaacaa gagcttctct gtgcttcaca acgtctgaat gaccatatca
     6961 atatgccatg ggtgatcctc tcttccagtg tcgacgaaaa actgttcccg cgtgccgtac
     7021 gcgtggcaat gacggcgggc gcatcgggat tcctggcagg ccgtgcagtc tgggcatcgg
     7081 tcgtcggttt accagacaac gagctgatgc tccgtgacgt ttgcgcaccg aaattacaac
     7141 aacttggcga tatcgtcgac gaaatgatgg ctaaacgccg ctaagatgag gataaaagga
     7201 atgaaatggt ttaacaccct aagccacaac cgttggctgg aacaggaaac cgaccgcatc
     7261 tttgattttg gtaaaaattc cgtagtgccg actggttttg gctggttagg caataaaggg
     7321 caaatcaaag aagagatggg cacccatctg tggatcacag ctcgtatgtt gcacgtttat
     7381 tccgttgctg cggcgatggg tcgacctggc gcttacgcgt tggttgatca cggtattaaa
     7441 gccatgaacg gcgcactgcg tgataaaaaa tatggcggct ggtatgcctg cgtgaatgac
     7501 gagggcgtgg tggatgcctc caaacagggc tatcaacact tctttgctct gctgggtgcc
     7561 gccagcgccg tcacaacggg tcacccggaa gcgcgcaagc tgctcgatta caccattgag
     7621 attatcgaga aatatttctg gagcgaagaa gagcagatgt gcctggagtc ctgggacgaa
     7681 gccttcagca aaaccgaaga gtaccgcggc ggcaatgcca atatgcacgc ggtggaagct
     7741 ttcttgattg tttatgacgt cactcacgac aaaaaatggc tggatcgcgc gattcgcgtg
     7801 gcttccgtga ttatccacga cgtcgccaga aataatcatt atcgcgttaa cgaacacttc
     7861 gatacccagt ggaatccgct gccggattac aacaaagata acccggcgca tcgcttccgc
     7921 gcgttcggag gtacaccagg ccactggatc gaatggggcc gtttaatgct gcacatccat
     7981 gctgccctgg aagcccgttg cgaacaacca ccagcatggc tgctagaaga tgccaaaggt
     8041 ctgtttaacg ccaccgtgcg cgatgcctgg gcacccgatg gagcggacgg gattgtttat
     8101 accgttgact gggaaggaaa accggtggtc cgcgaacgtg tacgttggcc tatcgtcgaa
     8161 gcaatgggta cggcctacgc gctctacacc gtcactggcg atcgccagta tgaaacctgg
     8221 tatcaaacat ggtgggagta ctgcattaag tacctgatgg actatgaaaa tggttcctgg
     8281 tggcaggagc tggatgcgga caataaggtc atcaccaaag tctgggacgg caaacaggat
     8341 atttatcacc tgctgcattg cctggtgatc ccgcgtatcc cgttagcgcc cggcatggct
     8401 ccagcggttg cagcgggtct gctggatatt aatgcgaaat aactgatctg aaaatagggt
     8461 gcgggttttc ccctcaccct aaccctctcc ccaaaggggc gaggggaccg tccggtgcag
     8521 tttttgggtt tgacatcagc ctcacgggag gctgtatgtc gttaattaag gtgccttgta
     8581 tgcaaatcac caatatgcat tgcagtggac agaccgtttc acttgccgcg ggggattatc
     8641 acgcgaccat tgtgaccgtc ggtgctggtc tggctgaatt aacctttcag ggttgtcatt
     8701 tagtcatccc gcacaaaccc gaagagatgc cgctggcgca tctgggtaaa gtgcttattc
     8761 cgtggccgaa ccggatcgcc aacggctgtt accgttatca ggggcaggaa tatcagttac
     8821 ccattaatga acatagttca aaagccgcta ttcacgggct gcttgcctgg cgagactggc
     8881 aaattagtga attaaccacc accagcgtta ccctgacagc ttttctgccg cccagttacg
     8941 gctatccgtt tatgctggct tcacaagttg tttattcgct caacgcacac acgggtcttt
     9001 ccgttgagat cgcaagccag aatatcggca ctgtcgcagc gccttatggc gtgggtattc
     9061 atccttattt aacctgcaac ttaacatctg ttgatgaata tctgttccag cttccggcaa
     9121 accaggttta tgccgtcgat gaacacgtaa atccgacaac gcttcaccac gttgatgagc
     9181 tggatttaaa tttcacgcag gcgaaaaaaa tagccgcgac aaaaatcgac cacaccttta
     9241 aaaccgctaa tgatttgtgg gaaatgacca ttacccatcc acagcaggcg ctttcggtaa
     9301 gcctttgttc cgatcaaccc tgggtgcagg tttatagcgg tgaaaaatta cagcgtcagg
     9361 gactggcagt tgaacctatg agttgcccgc ctaacgcctt taattcaggt atcgacttat
     9421 tattactgga accaggaaaa ccgcaccgcc tgttttttaa tatttacgga cagagaaaat
     9481 aacttgtatt gattagcagt caaaatcatt gaagaacatt tcactcgcta aaggcaacat
     9541 tatgtatctt caatttatta agttaaaaac agctcagctt ctgtaaagtt tttatatatc
     9601 agttttatga gaatcatttt acataagcct gtaataacag gaaggcaaca atctcgttct
     9661 caactctatg gagattagtt atggatacgc cacgtccaca gttattagat tttcaatttc
     9721 atcagaataa cgacagtttt accctacatt ttcaacaacg tcttatttta acccataaca
     9781 aagataatcc ttgtttatgg attggctcag gtatagcgga tatcgatatg ttccgcggta
     9841 atttcagcat taaagataaa ctacaggaga aaattgcgct taccgacgcc atcgtcagcc
     9901 agtcaccgga tggttggtta attcatttca gccgtggttc tgacattagc gccacgctga
     9961 atatctctgc cgacgatcag ggccgtttat tgctggaact acaaaacgac aaccttaacc
    10021 acaaccgtat ctggctgcgc cttgccgctc aaccagagga ccatatctac ggctgcggcg
    10081 aacagttttc ctacttcgat ctgcgcggca aaccgttccc gctatggacc agtgaacaag
    10141 gcgttggtcg caacaaacaa acctatgtca cctggcaggc cgactgcaaa gaaaatgcgg
    10201 gcggcgacta ttactggact ttcttcccac agcctacgtt tgtcagcacg cagaagtatt
    10261 actgccatgt tgataacagt tgctacatga acttcgactt tagcgccccg gaataccatg
    10321 aactggcgct gtgggaagac aaagctacgc tacgttttga atgtgctgac acatacatct
    10381 ccctgctgga aaaattaact gccctgctgg gacgccagcc agaactgccc gactggattt
    10441 atgacggggt aacgctcggc attcagggcg ggacggaagt gtgccagaag aaactggaca
    10501 ccatgcgtaa cgcgggcgtg aaggttaacg gcatctgggc gcaggactgg tctgggatcc
    10561 gtataacctc ttttggcaag cgcgtgatgt ggaactggaa gtggaacagc gaaaactacc
    10621 cgcaactgga ttcacgcatt aagcagtggt atcaggaggg cgtgcagttc ctggcctata
    10681 tcaacccgta tgttgccagc gataaagatc tctgcgaaga agcggcacaa cacggctatc
    10741 tagcaaaaga tgcctctggc ggtgactatc tggtggagtt tggcgagttt tacggcggcg
    10801 ttgtcgatct cactaatcca gaagcctacg cctggttcaa ggaagtgatc aaaaagaaca
    10861 tgattgaact cggctgcggc ggctggatgg ctgacttcgg cgagtatctg cccaccgaca
    10921 cgtacctgca taacggcgtc agtgccgaaa ttatgcataa cgcctggcct gcgctgtggg
    10981 cgaagtgtaa ctacgaagcc cttgaagaaa cgggcaagct cggcgagatc ctcttcttta
    11041 tgcgcgccgg ttctaccggt agccagaaat actccaccat gatgtgggcg ggcgaccaga
    11101 acgtggactg gagtctcgac gatggcctgg cgtccgtagt cccggcggcg ctgtcgctgg
    11161 caatgaccgg acatggtctg catcacagcg acattggcgg ttacaccacc ctgtttgaga
    11221 tgaagcgcag caaagagctg ctgctgcgct ggtgcgattt cagcgccttc acgccgatga
    11281 tgcgcaccca cgaaggtaac cgtcctggcg acaactggca gtttgacggc gatgcggaaa
    11341 ctatcgccca ttttgcccgt atgactaccg ttttcaccac cctgaaacct tatctgaaag
    11401 aggccgtcgc gctgaatgcg aagtccggtc tgccggtcat gcgcccgctg ttcctgcatt
    11461 acgaagacga tgcacatacc tattccctga aatatcagta cctgttaggc cgcgacattc
    11521 tggtcgctcc ggtacacgaa gaaggtcgca gcgactggac gctctatctg ccggaggata
    11581 actgggtaca tgcctggacg ggtgaagcgt tccggggcgg ggaagttacc gttaatgcgc
    11641 ccatcggcaa gccgccggtc ttttatcgcg ccgatagcga atgggcggca ctgttcgcgt
    11701 cgttaaacag catctaatca catccgcccg tggggaaact catgggcaaa gggagaacat
    11761 cgatgagtca catcacaacg gaagatccag caactttacg cctgcccttt aaagagaaac
    11821 tctcttacgg tattggcgac ctggcctcta acatcctgct ggatatcggt acgctttatc
    11881 ttttgaagtt ttataccgac gttctggggc tgccaggcac ctatggcggc attatctttt
    11941 tgatttcaaa attctttact gcgtttaccg atatgggtac cggcattatg ttggattccc
    12001 gacgcaagat cggtccaaaa ggtaagttcc gtccttttat tctgtatgcg tcattcccgg
    12061 tcaccttact ggcgatcgcc aactttgtcg gcacaccgtt tgatgtcacc ggtaaaacgg
    12121 tgatggccac tattctgttt atgctttacg gactgttttt cagcatgatg aactgctcct
    12181 acggcgcaat ggtcccggca atcaccaaaa accccaacga acgcgcctca ctggcggcat
    12241 ggcgtcaggg cggcgctacg ctgggcctgc tgctgtgcac ggtgggattc gtgccagtta
    12301 tgaatcttat cgaaggtaat cagaaacttg gctatatctt cgccgccacg ctgttttcac
    12361 tgttcggcct gctgtttatg tggatctgct actcaggcgt gaaagagcgt tatgtcgaaa
    12421 cccaaccaac caatccggcg caaaagcctg gcctgttgca atctttccgc gcaattgccg
    12481 gtaaccgccc actgttcatt ctgtgtattg ccaacctctg caccttaggg gcgtttaacg
    12541 tcaagctcgc cattcaggtc tattacaccc agtacgtact taacgatccc atcctgttgt
    12601 cgtatatggg atttttcagc atgggctgta ttttcatcgg cgtgttcctg atgcctggcg
    12661 cagtcagacg ttttggtaag aagaaggtct atatcggcgg cctgctgatt tgggtgctgg
    12721 gcgatctgct caactatttc ttcggcggcg gctcggtcag ctttgtggcg ttctcctgcc
    12781 tggcgttctt cggctcagcg cctgaactgg gcgctggttt ccgacaccgt cgagtacggc
    12841 gagtggcgca ccggcgtgcg ttcggaaggc acggtctaca ccggcttcac cttctttcgc
    12901 aaagtgtctc aggcgctggc tggtttcttc cccggctgga tgctgacgca aattggctat
    12961 gtgccgaacg tcgcacaggc tgaccacacc attgaagggt tacgccagtt gatcttcatc
    13021 tacccaagcg cactggcggt agtcaccatt gtggcgatgg gttgcttcta cagcctgaac
    13081 gagaagatgt atgtccgcat tgtggaagag atagaagccc gtaaacgcac ggcgtaataa
    13141 taataaacga cgccctgcgg ggcgttataa ggagtgatta tgtctgacca taatccactg
    13201 acattaaaac tgaatctgcg ggaaaaaatc gcctatggta tgggcgacgt cggttcgaat
    13261 ttaatgctct gcatcggtac tctgtatctc ctcaaacttt ataccgatga attagggatg
    13321 cctgcttact atggcgggat tatctttctg gtcgcgaagt tttttaccgc gtttaccgat
    13381 atgctcaccg gatttttact cgactcgcgg aaaaatattg ggcctaaagg caaatttcgt
    13441 ccctttattt tatatgcagc agttccggcg gcgttaattg caacgctcca gtttatcgcc
    13501 accacctttt gtttgccggt taaaacgacg attgccacca cgctgtttat gatgtttgga
    13561 ctttcataca gtctgatgaa ctgctcgtat ggtgcgatga tcccggcaat taccaaaaac
    13621 ccgaatgagc gcgcgcagct cgcggcttac cgccagggtg gtgcaaccat agggctattg
    13681 atttgtaccg tggcgtttat tccgttgcag tcgctttttt ctgactcaac cgtcggttat
    13741 gcctgtgcgg cacttatgtt ctccattggc ggctttattt ttatgatgct gtgctacaga
    13801 ggcgtcaaag agcattatgt ggacacagcg ccaaccggac ataaagccag tattctcaaa
    13861 tctttttgcg cgatatttcg taatccacca ttgctggttt tatgcattgc taacctgtgt
    13921 actctggcgg catttaatat caaactggcg attcaggtct attacaccca gtatgtgctg
    13981 aatgatatta atttattgtc gtggatggga ttcttcagta tgggatgcat cctcgtcggc
    14041 gtattactgg tgccagtaac tgtaaaatgt tttggtaaaa aacaggttta tttagctggc
    14101 atggtgctat gggcggtggg tgatatactg aattatttct ggggaagtaa ctctttcact
    14161 ttcgtcatgt tctcttgtgt cgcctttttt ggcacggcct ttgtaaacag cctgaactgg
    14221 gcactggtac cagataccgt agattacggt gaatggaaaa ccggtattcg tgccgaaggt
    14281 tctgtttata ccggttatac cttctttcgt aaaatttctg ccgcacttgc tggcttcttg
    14341 ccaggcatta tgctgacgca aattggttat gttcccaaca tcgcgcaaag cgatgcgaca
    14401 ttacagggtt tgcgtcagct catttttatc tggccttgtg cactggcaat tattgctgca
    14461 ttgacgatgg gattctttta cacactcaat gaaaaacggt ttgcattgat tattgaggaa
    14521 atcaaccaac gcaaaaataa agagatggcg acagaaaaaa aaacggcttc cgtaacgtta
    14581 taaatgtcaa tagccgctat ttccatcctg gtgggtggcg gcctccctac gtttaaaaaa
    14641 tggacttatt atgaaaaaga ttaatgcaat aattttatta tcttctctga cctctgcctc
    14701 ggtatttgct ggcgcatacg tcgaaaacag ggaggcatat aatcttgcct ccgatcaggg
    14761 cgaggttatg ctacgtgtgg gttataactt cgatatgggc gcgggtatta tgttaaccaa
    14821 tacctacacc tttcagcgag aagatgaact aaaacatggc tataataaaa ttgaaggttg
    14881 gtatccttta ttcaaaccaa ccgataaatt aaccatccag cccggtggct taattaatga
    14941 taagagtatc agttcgggtg gtgcagtgta tctgga
//
LOCUS       DAJTSA010000094        14479 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000094 DAJTSA010000000
VERSION     DAJTSA010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.094"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(113..379)
                     /locus_tag="OO369_003149"
     CDS             complement(113..379)
                     /locus_tag="OO369_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR5983324.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            653..4177
                     /gene="nifJ"
                     /locus_tag="OO369_003150"
     CDS             653..4177
                     /gene="nifJ"
                     /locus_tag="OO369_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR5983325.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4544..5673
                     /gene="ompN"
                     /locus_tag="OO369_003151"
                     /pseudo
     CDS             4544..5673
                     /gene="ompN"
                     /locus_tag="OO369_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5814..6248
                     /gene="uspF"
                     /locus_tag="OO369_003152"
     CDS             5814..6248
                     /gene="uspF"
                     /locus_tag="OO369_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR5983326.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6424..7359
                     /gene="ttcA"
                     /locus_tag="OO369_003153"
     CDS             6424..7359
                     /gene="ttcA"
                     /locus_tag="OO369_003153"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR5983327.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="OO369_003154"
     CDS             complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="OO369_003154"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR5983328.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="OO369_003155"
     CDS             complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="OO369_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR5983329.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10571..11803
                     /gene="dgcM"
                     /locus_tag="OO369_003156"
                     /pseudo
     CDS             10571..11803
                     /gene="dgcM"
                     /locus_tag="OO369_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="OO369_003157"
                     /pseudo
     CDS             complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="OO369_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12641..14095)
                     /locus_tag="OO369_003158"
     CDS             complement(12641..14095)
                     /locus_tag="OO369_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCR5983330.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14092..>14479)
                     /locus_tag="OO369_003159"
     CDS             complement(14092..>14479)
                     /locus_tag="OO369_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5983331.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3860 a         3652 c         3418 g         3549 t
ORIGIN      
        1 acaccattag cgaaatgctg aaagaaggtg cacaagtgga tctgaccgcg aaccagttaa
       61 cgctggcgac cgcgaaacag acattaactt ataagctggc ggatttaatg aattaatagc
      121 tgccacagct cccggcggca agtgactgtt cgctacagcg tttgccgttg ggtaatgcac
      181 acatcccaat cgccgtacca tccagttgac gggcaacaga aagcgaaccg ccgatcattg
      241 cacaatttgc ttctccacta ctggacatcg acgcttttaa acttggcgct acgtgcgccg
      301 cagtcgcctg ctgaacaggc tcactactac acgccgacaa caataaagcg gcacacccta
      361 cccaaaacgc tgctcgcatc tcttcttcct ctgatcttca agccaaacga caccgccata
      421 aataataggc agcacagagg gcggcgtcga gagctgtcct gcgcgttgcc ccgcattttt
      481 acttttttat ggctattttt ttgccctctg tttgatcaaa acattcatta ctctgatgtg
      541 ggggacacaa aagcgaaaat gcagaagaaa gccatttgct aaaattgaag gattactact
      601 gggcgcgcag caatttcgtg cgcccctcat ttgcgcaatg taagggtgtc atatgattac
      661 tattgacggt aatggcgcgg ttgcttcggt gacgtttcgc atcagtgaag ttatcgccat
      721 ctaccctatt acccccagtt ccacgatggc agaacaggct gatgcctggg ccggaaacgg
      781 cttgaagaac gtttggggag acacaccacg cgtggttgaa atgcagtcgg aagcgggtgc
      841 tatcgctacc gtgcatggcg ctttgcagac gggtgccctt tcaacatcgt ttacgtcatc
      901 gcagggtttg ctgctgatga tcccgacgct gtacaaactg gcaggcgaac tgacgccatt
      961 tgtcctgcat gtagcggcac gtaccgttgc cacacatgca ctttctattt ttggcgatca
     1021 ttccgacgtt atggcggtgc gccagacggg ttgcgcgatg ttgtgtgcgg ctaacgtcca
     1081 ggaagcgcaa gactttgctc tcatttcgca catcgcaacg ctgaagagcc gcgtgccatt
     1141 tattcatttc tttgatggtt ttcgcacgtc ccacgaaatc aataaaattg tcccgctggc
     1201 cgatgacact attcttgatc tcatgccgca ggccgaaatt gatgctcatc gcgcccgggc
     1261 gctcaacccg gaacatccgg tgatccgcgg tacgtccgcc aatcctgaca cttatttcca
     1321 gtctcgcgaa gccaccaatc cctggtacaa cgcggtctat gaccatgttg aacaggcgat
     1381 gaatgatttc gctgccgcga caggtcgtca gtatcagccg tttgagtatt acgggcatcc
     1441 gcaagcggaa cgggtgatta tcctgatggg ctctgccatt ggcacctgtg aagaagtggt
     1501 tgatgaattg ctaacccgtg gcgaaaaagt tggcgtgctg aaagttcgtc tgtaccgtcc
     1561 cttctccgcc aaacacttgc tgcaagctct gccgggatcc gtacgcagcg tggcggtact
     1621 ggacagaacc aaagaacccg gtgcccaggc agaaccgctc tatctggatg tgatgaccgc
     1681 actggcagaa gcctttaata atggtgagcg cgaaaccctg ccccgtgtca ttggtgggcg
     1741 ctatggtctt tcatccaaag aatttggccc ggactgtgta ctggcggtat ttgccgagct
     1801 caacgcggct aaacctaaag cacgctttac ggttggtatt tacgatgatg tgaccaatct
     1861 gtcactgccg ttgccggaaa acactctgcc aaactcggcg aaacttgaag ccttgtttta
     1921 tggccttggt agtgatggca gcgtttccgc aaccaaaaac aatatcaaga ttatcggtaa
     1981 ttccacgccg tggtacgcac agggctattt tgtttacgac tccaaaaaag cgggcggcct
     2041 gacggtttct caccttcgcg tgagcgaaca gccgattcgt tccgcttatc tcatttccca
     2101 ggctgatttt gttggctgcc accagttgca gtttatcgat aaatatcaga tggctgagcg
     2161 tttaaaacct agcggcattt tcctgcttaa tacgccgtac agcgcagatg aagtgtggtc
     2221 gcgcttgccg caggaagttc aggccgtgtt aaaccagaaa aaagcgcgct tctatgtgat
     2281 taacgcggcg aaaatcgccc gcgaatgtgg cctggcggcc cgtattaata ccgtcatgca
     2341 gatggctttt ttccatctga cgcaaattct gcctggcgat agcgccctcg cagaattgca
     2401 gggtgcgatt gccaaaagtt acagtagcaa aggtcaggat ctggtggagc gcaactggca
     2461 ggctctggcg ctggcgcgtg aatccgtaga agaagttccg ttgcaaccgg taaatccgca
     2521 cagcgccaat cgaccgccag tggtttccga tgccgcccct gatttcgtga aaaccgtaac
     2581 cgcggcgatg ctcgccgggc ttggtgatgc cctccccgtt tcggcgctgc cgccagacgg
     2641 cacctggccg atgggcacca ctcgctggga aaaacgcaat atcgccgaag agatccccat
     2701 ctggaaagag gaactctgta cccaatgtaa ccactgcgtc gccgcttgcc cacactcagc
     2761 tattcgcgca aaagtggtac cgcctgaagc gatggaaaac gcccctgcca gcctgcattc
     2821 gctagatgtg aaatcgcgtg atatgcgcgg gcagaaatat gtcttgcagg tggcaccgga
     2881 agattgcacc ggttgtaacc tgtgcgtcga agtttgcccg gcgaaagacc gtcagaatcc
     2941 agagattaaa gccatcaata tgatgtctcg ccaggaacat gtcgaagaag agaaaatcaa
     3001 ttacgatttc ttcctcaacc tgccagaaat cgaccgtagc aaactggaac gtattgatat
     3061 tcgtacatcg cagctgatta caccgctgtt tgaatattca ggtgcttgcc ccggttgtgg
     3121 cgagacgccg tatattaaat tgctgactca gctctatggt gaccggatgt tgatcgctaa
     3181 cgccaccggc tgttcttcaa tttatggcgg taacctgccc tctacaccgt ataccaccga
     3241 tgccaacggt cgtgggccgg catgggcgaa ctctctattt gaagataatg ccgaatttgg
     3301 ccttggtttc cgcctgacgg tcgatcaaca ccgtgtccgc gtgctgcgtc tgctggatca
     3361 atttgccgat aaaatcccgg cggaattact gacggcgttg aaatcagacg ctacgccaga
     3421 ggttcgtcga gaacaggtcg cagctttacg ccagcaactc aacgatgttg ccgaagcaca
     3481 tgaacttctg cgtgacgcag atgcactggt ggaaaaatca atctggctga ttggtggcga
     3541 cggctgggct tacgatatcg gctttggcgg tctggatcat gtattgagtt tgacggaaaa
     3601 cgtcaacatt ctggtgctgg atacgcaatg ctattccaac accggtggtc aggcgtcgaa
     3661 agcgacaccg ctgggtgcag taactaagtt cggcgagcac ggcaaacgta aagcgcgtaa
     3721 agatcttggc gtcagtatga tgatgtacgg tcatgtttat gtggcgcaga tttctctcgg
     3781 cgcgcagctg aaccagacgg tgaaagcgat tcaggaagcg gaagcgtatc cgggaccatc
     3841 gctgatcatt gcttatagcc cgtgtgaaga gcatggttac gatctggctc tcagccacga
     3901 ccagatgcgc caactcactg ctaccggctt ctggccgcta tatcgctttg atccgcgtcg
     3961 tgccgatgaa ggcaaactgc cgctggcgct ggattcacgc ccgccgtcag aagcactgga
     4021 agaaacgtta cttcacgagc agcgtttccg tcgtctgaat tcgcagcagc cagaagtggc
     4081 agaacagtta tggaaagatg ctgcagctaa tttgcaaaaa cgctatgact tcctagcaca
     4141 aatggccgga aaagcggaaa aaagcaacac cgattaaatg ctctggataa ggattatcca
     4201 attctaaaaa aaaagcccgg acgactgttc gggcttgtct ttttatatgt tggaaaatca
     4261 gtggcaatgc aatggcccaa cagaaaataa aatctgtgac aataaaggca tataacccgc
     4321 gcagaataac gtataggaaa ttaaaagtat aatttttatt ttgtatgtat tatcttcgat
     4381 acattcacca gacttattat catttcagag gaattatttg attaaggttt tacttaaggc
     4441 gtaacaaatg atatttatcg gctaactgaa cttctccttt aggatgtttt cactccccga
     4501 aatggggata aaaggcaaat aaaataacta aaggatttat tcaatgaaaa gaaaagtact
     4561 ggcactttta attcctgccc tgctcgccgc aggtgctgca catgcagccg aagtttataa
     4621 taaagacggc aacaaattag atctgtatgg caaagttgat ggcctgcatt atttttctga
     4681 taattcagcg aaagatggcg accagagcta tgcgcgtctg ggttttaaag gcgaaaccca
     4741 aattaacgat caacttactg gctacggtca atgggaatac aatattcagg caaacaacac
     4801 tgaatcttca aaaaaccagt catggacccg tctggcattt gccggactga aatttgcaga
     4861 ttacggttct ttcgattacg gacgtaatta tggcgtaatg tacgacatcg aaggctggac
     4921 cgatatgctg cctgaatttg gcggtgactc ttataccaat gcagacaact ttatgactgg
     4981 tcgagccaat ggcgtcgcga cttatcgtaa tactgatttc ttcggtctgg taaatggtct
     5041 gaacttcgcg gtgcagtatc aaggtaacaa cgaaggagcc agtaatggtc aggaaggcac
     5101 caataacgaa cgcgatgttc gtcatgaaaa cggtgacggc tggggtcttt ccacaacata
     5161 tgatttaggc atgggcttta gcgctggtgc ggcatacacc tcttctgacc gcaccaatga
     5221 ccaggttaac catactgcgg cgggtggtga taaagcagac gcgtggactg ctgggctaaa
     5281 atacgatgct aacaatattt acctagcaac catgtattca gaaacgcgta atatgacccc
     5341 gtttggcgac agcgattatg cagttgcgaa caaaacccag aactttgaag ttactagtac
     5401 cagtttgatt tttgtctgcg tccggcagtc tctttcctga tgtctaaagg ccgtgacctt
     5461 cacgctgctg gtggtgcaga caacccggca ggtgttgatg ataaagatct ggttaaatac
     5521 gccgatgttg gcgcgactta ctatttcaat aaaaacatgt ccacctatgt tgactataaa
     5581 atcaacctgt tggatgaaga tgacagtttc tacgctgcca acggcatctc taccgatgat
     5641 attgtcgctt taggtctggt ttatcagttc taaatcctcc tgcccgctgt tatggcgggc
     5701 tttttctgct tattcttcct cctttgatct aaattaaaaa tgtgaactcc gtcattacac
     5761 aaaaagtgtc atctggcgtt acactttatg cggatactaa aacaggaggt tttatgaaca
     5821 gaacgattct tgtccctatc gatatttcag attcagaatt aactcaacgc gtgattagcc
     5881 acgttgaggc agaggcaaag attgatgatg cagaggttca tttcctgacg gtaatacctt
     5941 cactgcccta ctatgcctct ctgggtttag cgtattccgc agaattaccg gcaatggatg
     6001 acctgaaagc ggaagccaaa tcgcaactgg aagagatcat taaaaaattt aaactgccaa
     6061 ccgacagagt gcatgtccat gttgaggaag gctcgcccaa agaccgcatt ctggaattgg
     6121 cgaagaagat ccccgctcat atgatcatca ttgcttccca tcgaccggat atcaccactt
     6181 atctgctcgg ttccaacgcc gcagctgtag tgcgtcacgc agagtgctcc gtgctggttg
     6241 tgcgctgaca ctaacgcccg cacattgctg cgggcttttt gattcatttc gcaaatgtgc
     6301 tgacattttc ccctctaatc cgtaccatac acgccacagt ttttatatca gatttcttat
     6361 gctgagcgtt ccggcatgac gcatactctt ctgatgccat ataacgaatt gagtcgcttt
     6421 taaatgtcgc aaaatcaaga aattagtaag aaagaacaat acaacctgaa caaattacaa
     6481 aaacgtctgc gtcgtaacgt gggcgaagcc attgctgact tcaatatgat tgaagaaggc
     6541 gatcgcatca tggtttgcct ctccgggggt aaagacagct ataccatgct ggagattctg
     6601 cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa cctcgatcaa
     6661 aagcaaccgg gcttcccgga acacgttctg cccgagtatc ttgaaaagct gggcgttgag
     6721 tacaagattg ttgaagagaa cacttacggt atcgtgaaag agaagattcc agagggcaaa
     6781 accacttgct cactgtgttc tcggctacgt cgcggtatcc tttatcgcac cgcaacggaa
     6841 ctggggacga cgaagatcgc gttgggtcac catcgtgacg atatcctgca aacgttgttc
     6901 ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc cgaaactgat gagcgatgat
     6961 ggcaaacata tcgttattcg tccactggcc tactgccgcg agaaagatat tcagcgattt
     7021 gccgatgcaa aagcgttccc gattattccg tgcaacctgt gcggttcaca gcctaacctg
     7081 caacgtcagg tgattgctga catgttgcgt gactgggata aacgttatcc agggcgtatc
     7141 gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga taccaacctg
     7201 ttcgatttca aaggcattac ccacggttct gaagtggtta acgggggtga tctggcgttc
     7261 gatcgcgaag agatcccact acaaccggcg ggctggcagc cagaagaaga tgaaaatcag
     7321 ttggatgagt tacggctgaa tgtggttgaa gtgaaataac caggatagcg cccgatgcgc
     7381 aagcgtatcg ggctactctt atggaggccg gataagacgc ggccagcgtc gcatctggca
     7441 atcccgaata agatgtttac tcttgcaccc ggcaattcaa catttcatta ttttaataac
     7501 cgcacccggc acgtttttcc tttaatcttc ccgccctgta actgtttcca tgctttatga
     7561 gcaacagcct gacggaccgc gacatagaca tgcgccggat gcacggcgat tttgccaata
     7621 tctgcgccat caagcccgat atctcctgtc agtgcaccta atacatcacc cgggcgcatt
     7681 ttggcttttt tcccgccatc gatacacaac gttgccattt ctgcttccag cgtcacaatg
     7741 gaactattag ctggcggcgt ttgccagtta agttttatct gcaacatgtc agaaatgata
     7801 ttggcccgct gtgcttcttc cggagcacag aaactgatcg ccagaccgct atttcctgca
     7861 cgagctgtac gaccgatgcg atgtacatga acttcagggt cccacgccag ctcaaagttc
     7921 accaccagct caagcgattt aatatccaga ccacgcgcag caacatcagt cgcaaccagt
     7981 acacgggcgc taccgttagc aaaacgtacc agggtctgat cgcgttgctc cagatcgccg
     8041 tgtaacgaca atgcactttg ctctacttca ttcagcgcgt cgcagacagc ctggcaatct
     8101 tttttggtat tgcaaaacac cacacaagag gatggctgat gcaagcttaa taaccgttgc
     8161 aacagaggaa ttttgccttt actggatgtc tcataaaatt gttgttcaat gggtggcaaa
     8221 gcatctgttg agtcaatttc aatcgccaaa ggatcgcgtt gcactcgtcc gctgattgcg
     8281 gcgatggctt ccggccaggt tgccgaaaac agaagcgtct gtcgagatgc aggcgcaaaa
     8341 cggatgacat catcaatggc atcgctaaat cccatatcca gcatgcggtc ggcctcatcc
     8401 atcaccaaag tattcagcgc atccagtgat accgtgcctt tttgcaagtg atccagcaaa
     8461 cgacccggcg ttgccacgat aatatgcggc gcatgttgca gcgaatcacg ctgcataccg
     8521 aacggttgac caccgcacaa cgtcaaaatt ttggtatttg gcagaaaacg cgccagccga
     8581 cgcaattcac ctgccacctg atccgccagt tcacgcgtag gacacagcac taaagcctgg
     8641 gtttgaaata gcgacgcatc aatttgctgt aacaagccga ggccaaaagc cgccgttttg
     8701 ccgctgccgg ttttcgcctg cacgcgaaca tcttttccgg caaggatcgc cggaagcgcg
     8761 gcggcctgca ccggcgtcat ggttaaataa cccaactcat taaggttcgt gagttgggcg
     8821 ggaggcaaaa cattcagggt agaaaaagcg gtcacaatct attctcgtgg tcatcgacgc
     8881 aaagttagca ggcgcgtatc ctcgcagatc tacgctcacg atgcgacaat ttaatcggtt
     8941 cttcatcggg tggtgggtca ggcatgggtt gcgggcgagg gatcggatcg ggcactggaa
     9001 caggatcgcc aggaatcggt tcagggacag gaatttgcaa ataaataagt gtcgtcatat
     9061 ttccctccgg tcattgggtg gactcttaaa gggtagacgc tgataaataa caggcaaaaa
     9121 aaagccgact catcaaagtc ggcgtcgtac gaatcaattg tgctatgcag taattcaaaa
     9181 aaggaagtaa gacaatatgg agcgcaacgc ccatcgcttg acgttgcatt cacctgcaag
     9241 ggagatattg ccctgaatgg gtagagagtt tattgacttc gctcaaactt tgcggcgttt
     9301 ttgtatacag acagccggaa aaattgcttt tgttacaacc atttactacg gtgcaaccat
     9361 aaagcaacac caccaataag aacaactaac agaatacaaa aaattgaaaa tccgaattgc
     9421 cacccgccgc cagggatccc accaaggttg acgccaaata acccggtcag aaaggtactg
     9481 ggtaaaaaga ccattgccat caacgacatt gtataggtac gacgagctaa attttcctgc
     9541 atcacctgag cgatttcatc cgccatcacg ccagtccgtg ctatacaggc gtcgatttcg
     9601 tcaaggccgc gcccaaggcg atcggcaata tcctgcatcc gacggcgttg gtcatcgctc
     9661 atccacggca aacgttcact ggcaagacga gcataaacat cacgttgcgg tgccatatag
     9721 cgacgcatca caattaattg tttgcgcagc agagccagga atccacgcgg tggaatttgc
     9781 tgatcaagga gattatcttc aaggtcgata attttatcgt gcagctgctc gataaattca
     9841 ctggaatgat cggtcaacgc atcgcacaca tccaccagcc atcccccgca atcggtcgga
     9901 cccgtgccct cttccagatc gctcaccaca tcgtccagcg ccagcacttt gcgttgtcgg
     9961 gtcgaaacaa ttaaccgccc gtccatatat acacgcatgg cgaccagttg atcggggcgt
    10021 tcatcggtgc tgccgtttat acagcgcaat gtaatcagcg tgccttcacc gaaacggctg
    10081 actcggggcc gtgtgctctc gcccgccagc gcatcacgta cgttattggg aagcagcggt
    10141 gttgtcgcca gccattgggc gctatcatgg tgtacataat taaggtggag ccagcaggga
    10201 tgcgcttcat caatcacatc tgtattttcc agcggtttaa cgccgcctct accatccagc
    10261 atccaggcaa atactgcatc cgggacatta acgtccgatc ccttaatcgc ttccactgtg
    10321 cctccatcat caacgcatta ttttgtagtc tagccttctg gccctgttac gcaacatctc
    10381 atcaccccat taccctgaaa tgattaataa aattctgtct aaattgaata caaaaagcaa
    10441 aatgcttttc cgtatacaaa ccgtgtgaag tgttaaatag cgtctatcat tatcagaatt
    10501 atctgatcat atgacgtggc ttttttgcga tcggatagca acaaaaattg ataaaaataa
    10561 cgggatctca atgattacgc acaacttcaa taccctggac ttactcacca gtcctgtctg
    10621 gattgtttcg ccctttgagg aacagttaat ttatgccaat agcgcggcgc gactgttgat
    10681 gcaagacctc acgtttagtc agctacgaac cggaccctat tccgtctcct cacaaaaaga
    10741 actgccgaaa tacctctccg atctgcaaaa ccaacacgat attatcgaaa tcctcactgt
    10801 tcagcgtaaa gaagaggaaa cagcattaag ctgtcggctt gttttgcgag agctgacaga
    10861 aacagaaccg gtgattattt tcgaaggtat cgaagcgccg gcaacgctgg gtttaaaagc
    10921 cagtcgctcg gcaaattatc agcgcaaaaa acaaggtttt tatgcgcgct tttttctgac
    10981 taactctgca ccaatgttgt tgattgaccc gtcacgagat ggacaaatcg tcgatgctaa
    11041 cctcgccgcg ctcaatttct atggttataa ccatgaaacg atgtgccaga aacataccta
    11101 ggaaataaat atgcttgggc gtcgcgtcat gcctatcatg catgaaattt cgcatttacc
    11161 cggcggtcat aaccctttga attttgttca taaactggcg gatggatcta ctcgtcatgt
    11221 gcagacctat gccggaccga ttgaaattta tggcgacaag ctcatgttat gtattgtgca
    11281 tgatattact gagcaaaaac ggctggagga gcagctggaa catgctgctc accatgacgc
    11341 gatgaccgga ttactgaatc ggcgacagtt ttatcacatt acggaaccag gccaaatgca
    11401 gcatctcgcc atcgctcagg attacagctt gttgctcatc gacaccgatc gttttaaaca
    11461 cattaacgat ctctatgggc attctaaagg tgatgaggtg ttatgcgccc tcgcccgccc
    11521 cctcgaaagt tgcgctcgca aaggcgattt ggtgtttcgt tggggaggcg aagagtttgt
    11581 cttattgcta ccaagaaccc cactggatac cgcgctttcg ctggctgaaa ctatccgcgt
    11641 aagcgtggca aaagtgagta tttcgggctt accacgcttt accgtcagca ttggtgtggc
    11701 gcatcacgaa ggaaatgaaa gcatcgatga actgtttaaa cgcgttgatg atgctttgta
    11761 tcgggcgaaa aatgatggac gcaaccgcgt gctggcggca taagccgcgg atgcgtctcg
    11821 agatcaacga ctgcgcttag cgtggcgctc ccagttttct tgcttcgcct gcgccgtttt
    11881 acgtagtgcg acgtaacacg ccccgctgcc gccatgatgc ggtagcgcgg tgcaatatgc
    11941 ctgaacatca tcaaattcgg tcagccagcg cgccacatag ctgcggacaa tattggcatg
    12001 tgatttatct tcccgccctt taccatgaat aatcagcaca ttacgcaaac catccgccag
    12061 ggcttgttga atgaaactga acaccatttt gcggcactct tccaccggct ggcgcaaaag
    12121 attcaggctc gcctgttgcg gatatttacc actgcgcagc ttatccagca ccccatgcta
    12181 caacccttcc tgccgaaact ccagcggctg acttagcggg atgatgtcga gaaatccggt
    12241 agtgaggaaa ttatcaagct gcagcgtgtc gatacgctgc ggcgcacgtt ggttacgcgt
    12301 tggatgccag tggacatctg tagcacgttt cagcggctgg acatcttcca tggcgtcaag
    12361 aaacagcgat ttgtcgtcaa ggttcatgta acctcctatg caccttcaca cgtcgtgatt
    12421 cagacgggtt aattgttcat acttactgca tcgttccctt cacagattga aacaatactc
    12481 tacttcatac ctgtgagtat ctgcgcaggc ggggcatttt atactagcct gttagcagta
    12541 tcgaacagtt tagcccgagt ttaattcagc acaggctccg atcaggatga tttattcata
    12601 gcaggaacca acaaaaatgc gataaataac agaccactga ttagcagaaa ggtaccgaaa
    12661 atgccatagt ttgcgcctcc ctcagcatca attgactcaa taatttggtt gaagccagta
    12721 tttccgatgt ctggtcactt ctactgcgat taaaaaataa agcacttctg gaaatgaagt
    12781 cattcaccgt atggggaaca gaaaccattt tttcacccat tctcgaaaaa tgaaaattag
    12841 ttcgattatt gagaatagag ccacacaggg caataccaat cgcgccccca agattacgtg
    12901 ttaaattaaa tacaccagag gctaacttca gccgttcttt tggaattccc cccagcgtta
    12961 atgttacgat gggggccatc gcaaactgct gcgaaatccc tctaattgcc tgtggaaaca
    13021 gtaattcctg ccatccccat tcgtgggtta taggggtaaa aagatacatt gaaaacacaa
    13081 atccgcccag ccccgccatc aacagccatc gcagatttat ttttttactt aaccagaagt
    13141 aaaagggaac agaaaataac tggaaaatac ctgttgtgca aacggcaaaa cctatctctt
    13201 cggcatttaa tccgcgaatc tgccccaaaa acacaggtat taaatataca gtagagaata
    13261 tcccaacccc tccagaaaaa gaaaaaaaac atcccagagt gaaattttta tccttaaaag
    13321 catgaagatc cattatcgga ttggatattg taagtgtacg cgcagcaaat aaaataaaac
    13381 tcaccaatgc cagaactgat gtcagcaaaa tagtattatc atccagccat ccccagcgcg
    13441 ctccttcttc aagggtatat tccagacatc ccagagtcat ggccaacagg ataatactcg
    13501 gataatcagc aactttcagt aaagagagat caggtttatc aaaattaacc agaaaaggaa
    13561 tactcaaaac taaatatatg ccaggtaata tattgatata aaatagccat cgccagtcga
    13621 ggttatcagt gagccagccc cccagagttg gccctagcgt cggtgacagt gaggccagcg
    13681 cactgactac cgcagcagcc aaccccaact ctttaccctg gtaataaata aacgccatcg
    13741 tgaaaactaa agggatcatg gatgcgccag cagctccctg taaagcacga aacaaaatca
    13801 tgatctgaat attccatgca agtccacacg cgatactcat taaagtaaat attcccgcgg
    13861 atagagtaaa aagccaacga gtggaaaata cccgtgacag ccacccagag agcggaataa
    13921 caataatctc agcaattaaa tagctggtct gaagccaggc catttcgtct ttacctgcgg
    13981 acagaccacc acctatttca tttagggatg acgatacaat ttgaatatca agctgagcaa
    14041 taaataatcc catacacata ctggcgaaag caatatgttt acgcatagac atcatctctc
    14101 atcaacactg acggtgacgg acagtcccgg gcgaagaagt tgctttaatt cctttgcgtc
    14161 gtcaaatgca atccgtaccg ggacacgctg aacaattttg gtgaaattgc cagtggcatt
    14221 ttctatcgga atcaagctga aactggctcc tgtcgctggg gaaaggcttt caatgtgacc
    14281 gtgaaacact tcccctttga gaatatcagc acggatctca gccttcattc ctggcttcat
    14341 tcccgctatt tggttttctt tataatttgc atcaacccac aactcactga ctggtactag
    14401 tgaaactaac gatgtgccgc cttccaccca actgcctgta tgggcgctgc ggtttgcgac
    14461 aatgccatca atcggcgca
//
LOCUS       DAJTSA010000095        14421 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000095 DAJTSA010000000
VERSION     DAJTSA010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.095"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..132)
                     /locus_tag="OO369_003160"
     CDS             complement(<1..132)
                     /locus_tag="OO369_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5983332.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     "
     gene            315..458
                     /locus_tag="OO369_003161"
     CDS             315..458
                     /locus_tag="OO369_003161"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983333.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(677..2215)
                     /gene="aldB"
                     /locus_tag="OO369_003162"
     CDS             complement(677..2215)
                     /gene="aldB"
                     /locus_tag="OO369_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR5983334.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2380..3531)
                     /gene="yiaY"
                     /locus_tag="OO369_003163"
     CDS             complement(2380..3531)
                     /gene="yiaY"
                     /locus_tag="OO369_003163"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR5983335.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(3721..5565)
                     /gene="selB"
                     /locus_tag="OO369_003164"
     CDS             complement(3721..5565)
                     /gene="selB"
                     /locus_tag="OO369_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR5983336.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(5562..6953)
                     /gene="selA"
                     /locus_tag="OO369_003165"
     CDS             complement(5562..6953)
                     /gene="selA"
                     /locus_tag="OO369_003165"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR5983337.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(7051..7659)
                     /locus_tag="OO369_003166"
     CDS             complement(7051..7659)
                     /locus_tag="OO369_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR5983338.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(7730..8815)
                     /locus_tag="OO369_003167"
     CDS             complement(7730..8815)
                     /locus_tag="OO369_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5983339.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(8818..9180)
                     /locus_tag="OO369_003168"
     CDS             complement(8818..9180)
                     /locus_tag="OO369_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5983340.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            9717..11630
                     /gene="mtlA"
                     /locus_tag="OO369_003169"
     CDS             9717..11630
                     /gene="mtlA"
                     /locus_tag="OO369_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR5983341.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            11725..12873
                     /gene="mtlD"
                     /locus_tag="OO369_003170"
     CDS             11725..12873
                     /gene="mtlD"
                     /locus_tag="OO369_003170"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5983342.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            12873..13460
                     /gene="mtlR"
                     /locus_tag="OO369_003171"
     CDS             12873..13460
                     /gene="mtlR"
                     /locus_tag="OO369_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR5983343.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(13472..13681)
                     /locus_tag="OO369_003172"
     CDS             complement(13472..13681)
                     /locus_tag="OO369_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983344.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            13965..14327
                     /locus_tag="OO369_003173"
     CDS             13965..14327
                     /locus_tag="OO369_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR5983345.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         3523 a         3842 c         3624 g         3432 t
ORIGIN      
        1 aatggcgtcg gcatgaggat gattacgttt tttagcaatc aggtagggaa tatcatgtaa
       61 aataataatc ccatagaata tcaccagaaa tacaaaaata agcactccca gtgcaaaata
      121 gtccaggaac atatttccct caaagaatat aaaaaagaac aattaacgca tattatgaaa
      181 tgccatgatg cagtgcaatt tccttattta tataacgaca tattgttttt tatatacttt
      241 ctgaaatgtg ctgtctggtt tttcaacagg acgaggttta tcatattgaa atagtggaac
      301 tattggccaa actaatgaat aactccagta aaacatccac agtacagatt aagcgtatta
      361 aaccttcaat tatctaccgt ttattgctga ttggcctcgg atcaccaatg gtgatttacg
      421 gcctggttcg cccgctcacc atcgaaacgc gagattaaac tcctgacaaa acacgcccca
      481 gaaacgcact tctgttggcg tagtctgggt tattgcgcac gtaggtttaa aacgtaaatt
      541 gtaacaacgt gcgctttgtt tatgccggat gcggcgtgta cgccttatcc ggcctacggt
      601 caggttcccg taggcatgat aagacgcgta agcgtcgcat caggcaatga atacccaatg
      661 cgaccagctt cttatatcag aacagcccca acggtttatc cgagtagctc accagcaggc
      721 acttggtttg ctggtaatgc tccagcatca tcttgtgggt ttcgcgaccg atacctgatt
      781 gtttgtagcc accaaacgca gcatgtgccg ggtaagcgtg ataacagttg gtccacacgc
      841 gcccagcctg tatgccgcgc cccatcttat aggccagatt accgttgcgg ctccagacgc
      901 ccgcgcccag gccatattgc gtatcgttcg ccagctccag cgcttcttcc atcgttttga
      961 aggtggtcac cgccagcacc gggccaaaaa tctcctcctg gaacacccgc atattgttct
     1021 gaccaaacag aatcgtcggt tcgaggtagt agccgtcttt cagttcacct tccagcagct
     1081 tgcgccgccc gcctgtgagc acgtcagcgc cctctttttt accgatatca atgtagttga
     1141 ggatggtttc cagttgcccg tgagaaacct gcgcgcccat ttgcgtcacg ctgtcgagcg
     1201 ggttaccgct gcgaatgctt tcgacacggc ggatggcgcg ttccataaag cgttcgtaga
     1261 ttgactcctg cactaaggca cgactcggac aggtgcaaac ttcgccctgg ttaaaggcaa
     1321 acagtgcaaa gccttccagc gctttatcga aaaaggcatc ttcttcatcc atcacatcag
     1381 caaagaagat atttggcgac ttaccgccca actccagcgt caccggaata atgttttgcg
     1441 ttgcgtattg cataatttgt tggcccactt ccgttgagcc ggtaaacgcc actttggcga
     1501 tgcgtttcga ggtcgccaga tattcgccaa tttccccacc tgcgccattg accacgttca
     1561 ccacgcccgg cggcagtaaa tcaccgacaa tttccattag cagcagtaca gaaagcgggg
     1621 taagacgtgc gggtttcagc accacacagt tgcccgccgc cagcgcggga gccattttcc
     1681 agctcgccat cagcagcggg aagttccacg ggataatctg ccccaccacg cctaacggtt
     1741 catggaaatg ataggccacg gtttcgctat caacttcact gatcccacct tcctgcgccc
     1801 gaatacacga ggcgaaatag cggaaatggt caatcgccag cggtacatcc gcagcactgg
     1861 tttcgcgaat gggtttgccg ttatcccagg tttcagctgt cgctaacagc tcgaggtttt
     1921 gttccattcg atcggcaatc ttaaacagaa tcgccgcgcg atcctgcacc gaggtgtgcg
     1981 cccatttatc tttcactttg tgcgcagcat ccagcgccag atcgatgtct cgtttgcccg
     2041 aagacgccac ttcgcacagc agctgcccgg tcaccggcgt cagattctgg taatactcac
     2101 catcggcagg ggctacccat tcaccgccaa taaagttgtc gtagcgggct tttaacttga
     2161 tggggaaacc atactcgccg ggcttaatct gtgctgaagg gggattattg gtcatgatcg
     2221 tctccttgcg gtgtgaggta taacaagggt agacgtgact ggcgaaatct tcgccagtcg
     2281 gtaacagctt tacgacagct atcacgaatt tacgggcaag gattacagag atagttgaaa
     2341 aagcggctga caatttgcca gccgttgttg gaatgatgat tacattgcgg cgcgataaat
     2401 caccacaatt tcttcgtgag ttgcctggat cgggttagta aagccacagg catctttcag
     2461 ggcattagtc gccagaaccg cgaaatcttc ttctttcacg ttcaggtcgc gtaggcctgc
     2521 cgggatatcc actttcttcg ccagttcacg gatggcgtta atgcaggctt cagcaccttc
     2581 cgcgtcgttt ttacctgtca cgttcacgcc cattgcagcg gcacagtcac gcagacgtgc
     2641 agcggcgact ttgctgttga atacctgaac atgcggcagc agaacggcgt tacatacacc
     2701 gtgtggcagg ttgtagaaac cgcccagctg gtgcgccatc gcatgaacat aacccagaga
     2761 agcattattg aacgccatac cggcaaggaa ctgggcataa gccattgctt cacgcgcttt
     2821 cgcattactg ccatcttcaa cggctaacgg caggttttcg gcaatcatgg tcacggcttt
     2881 cagtgcacaa gcgtcagtga tcggcgtggc ggcaatagaa acatacgctt cgatagcgtg
     2941 cgttaaggca tccatacccg ttgcggcggt cagtgacttc ggcataccaa tcatcagaga
     3001 ggagtcattg acagaaagca gcggagtcac gtgcttatca acaatcgcca ttttgttatg
     3061 acgcgcttcg tcagtgatga tgcagaaacg ggtcatttca gaggccgtac ccgccgtggt
     3121 attgatggcg atcatcggca gctgcggttt tgcagagcgg tcaacgcctt cgtaatcgcg
     3181 aatatcgccg ccattggctg ccaccagcgc aatacctttt gcgcagtcgt gtggagaacc
     3241 accgcctaag gagatcacgc tatcgcaatt attttcttta agtaatttca aacctgcggc
     3301 gacgttttcc gtggtggggt taggttgggt gccatcataa ataacgctaa aaatattgcg
     3361 ttcttccagt gctttttgca catcacccgc catacctaat ttcgttaaca tattgtcagt
     3421 gacaattaag gtacgggtaa atccataatc tgccatcata ttcattgcat cagtcaatga
     3481 atcagcgccg atgacattca cagaaggaat aaagaaagtt gaagatgcca taacacactc
     3541 ctgaaagtgt aaaaaagggt gggcagctta ctgaggattt tcattcgaaa acatgatcat
     3601 tattattaat tatcaaaatt aatctaataa aaagcacatt gtattcatta aacaatgtgc
     3661 tttatttaaa atcataagaa atttgtatta atttatttta aaaattaatt aatcatttcc
     3721 ttatttttcc ggaaataata atgcgtcgcg caataaatga tcatttccac gacgacgcgt
     3781 aaagccaatg cggtcaaaat attccagaat ctgaattgcc agctttcggc ctacgcctaa
     3841 gcgatcgcgg aaatccgccg cgcaggttga accacactcc tgatcgagat cgcggatcat
     3901 attggcaaat tcgacaatcc gatcgttacg gtaataacga tctttgacga tcgcggtaat
     3961 tattccttgc tgcgccgcct ggcgtagcgt caggcgcatt gcctgctcgt cggtacccgt
     4021 ctcttttgcc agatcacgca cccaccacgg ctcgtcacca aacagcggct ctgctttttg
     4081 ccagatggcc tgctgctctt cgctgaagcc cgctttgtga tccggcagat gcagccaacc
     4141 gtgatgactg aggatgtcgc cgctttcgcg catcttttca atcagcaaca gtactagcgc
     4201 ttcatcttcc attggcaacg ccatacgtcg cagacgttcg cgcccaggcc caggttcatc
     4261 gcgatgttgc tcatgataag tcgctaatgt gtcgagaatt ttccgttgcc agcgagcggc
     4321 aaccggcgca ttcaacaagc tataaccagc ctgaatatag ccaggctgtt gcagcagttc
     4381 gcgcatccct tcgccgttga gctggcgcgc ccaagcgaaa tcaggaagat taaccgcgcc
     4441 gcgttgcagg tgaacggcta acgcttcgct atcattctgt gccctggcga gggaagccag
     4501 ccattgcaga tactctggtt tacgtttacc gcgacgcggc gggttaagca tcacgacgcg
     4561 cgctccggcc agcgtgttgc gggcagagat atcgcgcaat accaggcggt cgttatctgc
     4621 cagccataac ggggtgtcga agaccagttc agcaaggtta tcttccagca gtgaaacgcg
     4681 tcccgtgacg tggctggcgg cgtggtgaat atgcagcggc tgccactggg tcagcggtgc
     4741 atgggtttga agctcgacaa tcacccgtgt gaacggctct ggcggcacat cggcaagcag
     4801 ccagtcgcca cggttaatct gctctttttc cgcatcaccc gcgatgtcaa gcgcgatacg
     4861 ctgcccggca ttggcggttt ctgttggctg gttttgcgca tgcagcgcac gtacacgcat
     4921 cggtttattt acaccagtca gccagagtga atcgcctacc ttcacttccc cgcttaacgc
     4981 cgtaccggtg acgaccagcc cggcaccttt tacggtaaat gcgcggtcaa tcgcgaggcg
     5041 gaaactatgt tggctggcgt gctcgcgttc cggcaactga agcagatgct cgcgcagggc
     5101 atcaattccc cgaccttcgg ttgctgcggt gataaacagt tttgcctcag caaaaccgta
     5161 ttcccgcaga acctccttta cctggcgttc aacctcatca acacgcgctt cgtccacgcg
     5221 atcggctttg gtcagcgcca ctgtcagcat cgggttaccg gtcagctgca aaatcgccag
     5281 atgctcacgg gtctgtgcca tcacgccgtc atcgcacgcc accaccaaca gcgcgtgatc
     5341 gataccacca acgcccgcca gcatgttgga aagaaacttt tcatgaccgg gaacgtcgat
     5401 aaaaccaggc acgcgaccat ccggctgcgg ccagtaggca tagccgagat cgatggtcat
     5461 gccgcgcttt ttttcttccg gcagacggtc agcatttacg ccagtaatcg cctgcaataa
     5521 ggttgttttg ccgtggtcaa cgtgtccggc agtcgcaata atcatttcaa caacatctcc
     5581 aaaaaccgtt gctcatcttc aaggcagcgt aaatccagcc acaatcgtcc gtcataaata
     5641 cgaccaatca ccggcactgg caattcacgc cagcgggcgg ctaatgactc aaggtggcta
     5701 ccgcgtccat catggggtgt aaacgttaat gccgcgctcg gcaggcgatc aaccggcagc
     5761 gaaccactgc caatctgcga aagacatggc ataacctgta ccgcaaactc cgcgccgtaa
     5821 tgtgcgacaa ggggagcctg taaacgttgt gcctggattt gaatgacctc tgcgctgcgg
     5881 gtaagcaggc gcagggtcgg taatttttca ctcagagctt cagggtgtaa ataaagacgc
     5941 aacgtggctt ccagcgccgc gagggtcatt ttatccgcgc gtaatgcacg cttcagcggg
     6001 tggctttgca ggcgggcgat catctctttt ttaccaacga taatcccggc ctgcggcccg
     6061 cctaacaact tgtcgccgga gaagctcacc agactgacgc ccgccgcaat caactcctgc
     6121 ggcattggct ctttcggcaa accgtactgg ctaagatcga ccagcgagcc actgcctaaa
     6181 tcagtcacca ctggaacatc cagctctttg ccgagcgcca ccagttcagc ttcgtctatc
     6241 gctttggtga acccctgaat gctgtagtta ctggtatgta ctttcatcaa cagtgcggta
     6301 ttttcattca ccgcctgacg ataatcattc gcgtgcgtgc ggttggtggt ccctacttcg
     6361 tgtagggtgc agcctgcctg acgcataaca tcgggaatac gaaacgcgcc gccaatctcc
     6421 accagttcgc cgcgagatac caccacctct ttcccgctgg cagtggccgc caacatcaat
     6481 aacaccgccg ccgcattgtt attgacgata caggcatctt ccgcccccgt aatacggcac
     6541 agcagctgcg ccagcgcccg atcgcgatgt ccgcgtccgg cgtcgtccag atcgtactcg
     6601 agggtcactg gcgaacgcat agcctgcgca acggcttcca ccgcggcttc cgcctgtaaa
     6661 gctcgcccaa ggttggtatg cagcacggtt cccgtcaggt tgatcaccgg acgcagcgcg
     6721 ctctgcgcct ctttcgtcaa ccgggcatcg acttcttgcg cccagttttc acaccacgca
     6781 ggcagcgtct ggctgccacg aatcacttct cgcgcttcgt cgagcatctg acgcaacaat
     6841 tccaccacgc gggtgtgacc ataagtatca cgcaaagaaa ggaaggagct atcgcgcaat
     6901 aagcgatcaa tagccggaag ttgactatag agggaacgcg tttcggttgt cataggaaac
     6961 ctggctgatc aaggccctct cacacggaga agggcgttta acataaccac ggattgtaac
     7021 gtgagatggg tcaggaggac atatcgcgca tcaagccttt ggcggttcgg tgcgagcaaa
     7081 actttcgcgg ctaaacaggt tttcgaccag tttgactaag tgtgggcggt cgacacacca
     7141 acctggcgca acgcggcgga aattgagata tccgacagca caggcaatgg cgatagtcgc
     7201 cagattgacc gtatcggttt tgagtgtgcc atcgaccaga tatccttcca gcacatccag
     7261 actgcggttg attttctccc gctggcgtaa caattcatct tcagactgct gcgccgccgg
     7321 acgcgcctgt tcacgcaccg ataccagccc ggcatccata atgccatccg ccagcgcctc
     7381 aattttgcgc acccgcaacg actccagcgg atcgcgcggc aacatcgccg gagcgacatt
     7441 cattaactca atatattcag cgatgatcgg cgaatcaaac cagcattcgc cctcttcggt
     7501 caccagcacc ggcactttcc ctaacgggtt aaattgcgcc acgccgttat ccgcgttata
     7561 gggcagttca ttaataaatt cgaaagttat gcccttttct aacaacagaa taaaaagttt
     7621 gcgtacaaac gggctggtgt agctaccgac gagtttcatg ccgagtcctt tgtgcgagga
     7681 aaaatatcag tatggctcag gctcacgcaa aacacaggtg atataagtct caatgatcca
     7741 aataaagata atgcatccaa ctggtcattc ttagcagcac tttccgcatc accgaaacat
     7801 ggctgaaatg gtcggagtaa accgccacct gggcgtagat gccgtcgggt aaggcatcga
     7861 tatcatcgtt agggtccagt tcaatggttc ccagcacacc gtccgtgccg ggaacgaccg
     7921 ttaatgattg caataccccc tgcgcctgat aagaaccgcc tggcacgaca ggtaaaatac
     7981 tagtcaattt gccgtggaac acctgcccag gtagcgcgtt aaacaccact tccgcatcat
     8041 cgccaggttt cagacgtaac agcgagtttt gccgaaattg ggcgacaatt tgccgttttt
     8101 gctcggggat gaagaccatc accggacgca gcggcaaggc agctgcgtat gtacctgggc
     8161 ggatcagtac ctgagtgacg tagccattgc ttggtgcgcg aatgacagtc tgctcaagat
     8221 tatattttgc ttcagtaagt tgcgctctta agctcacaat ctgcgattgc tcgccgttga
     8281 ccatactgtc gagctgactc tggatctgcg cctgctccgc caccgagcct ttcactaacg
     8341 catcctgcgc gaggaaattt tgccgcgcat cgtcgatgtc acgttccgag aacggattca
     8401 ccgccgcctg gctgcctttc aggtaacgtt gataattttt aaacagacgg tcgcgctccg
     8461 ctgaaacctg ggtggtgttg gcctgcgctt cagtgagctg cgcacgcagc gtctttatat
     8521 tatgcgtcgc cgtcatcagg tcagcctgaa gtctgtcaac tcgcgcctgg taacgaaccg
     8581 ggtcgagctt aaaaagcacc tcgccctttt gaataagctg attattcttg tcagtgactt
     8641 cagtaacaat tcccgtcacc tgtggcgtga tagggatcgc tatcactgcc ttttgcgcgg
     8701 taaaagtgta agggtggttg tagttcatca acaaaatcaa accactcacc agaaacacgc
     8761 ctcccagcgc cgccgtcgcc agcgccacgt aagttaaaac aatcaataga tccataatta
     8821 gatctccggg aacgtggaat agtcagtctt tttctcggcg gcgagttgat gctccagccg
     8881 ggaaattcgg tcagaaagtg cggcgatttc cggttcagtc gctttctcct gcgacgcaac
     8941 atgtgactgc ataccccagc cacgctccgg ttgatagagc gtcgcccaga tccacagaaa
     9001 cggccagata acatgcagag taaacaggct cacccagccc gccgtatgaa tagcgtcggc
     9061 atgggggtga ttgcgctttt tggcaatcag ataagggatg tcatgtatcg cgatgattcc
     9121 ataaaaaatc accagaaaga cgaagatcag cactcccaac gcgaaatagt ttagaaacat
     9181 atctgcctcg gattcacgtt tatcagtgtt gttttttggc tggcagccag aagggagtca
     9241 ggctgatatt ttgacaataa tccgggttcg cgattctcgc cataacacca aagaataatt
     9301 tttagaggtg atgagttgct tagttacata acgattgtat gacgaaggca taacatgctg
     9361 tagatcacat caggtgaacg ccgtaagaaa atatcttgtg attcagatca caaagattca
     9421 acaaaccatc aaaacaaaaa tgtgacatta ctcacattta aatgccattt ttagcgaaaa
     9481 tcaccgcctt gtttcttttt tatacaggcg ttttgtgatg agcgtcacgt caattacctc
     9541 tctaccccct atatttatgt gactgatatc acacaaaagc ccgtcgactg gacagttaac
     9601 cgattcagtg ccagatttcg cagtatctac aaggtccggc tacctctgcc gccacattaa
     9661 caaaaaacct cgggcttcca gcctgcgcga cagcaaacat aagaaggggt gtttttatgt
     9721 catccgatat taagatcaaa gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc
     9781 caaatatcgg cgcgtttatc gcgtggggta tcatcaccgc gttatttatt ccaacagggt
     9841 ggttaccgaa cgagacgctg gcgaagctgg tcgggccgat gatcacttat ctcctaccgc
     9901 tgctgatcgg ttataccggt ggtaagctgg taggcggcaa acgtggtggc gtagtcggtg
     9961 ccattaccac catgggcgtt atcgtcggcg cagacatgcc gatgttcctc ggttctatga
    10021 ttgcaggtcc gctgggcggc tggtgcatta agcacttcga ccgctgggta gacggtaaga
    10081 tcaaatccgg ttttgagatg ctggtgaata acttctccgc aggcatcatc gggatgatcc
    10141 tcgctattct ggcattcctc ggcattggcc cgattgttga agccctgtcc aaaatgctgg
    10201 ctgcgggcgt taacttcatg gttgtccatg acatgctgcc gctggcgtct atctttgttg
    10261 aaccggcgaa aatcctgttc ctcaacaacg ccattaacca cggtatcttc tcgccgctgg
    10321 gtattcagca gtcccatgaa ctgggtaaat caatcttctt cctgattgaa gctaacccag
    10381 gtccaggtat gggcgtgctg ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt
    10441 ctgcgggcgg tgcggcaatc atccacttcc tgggtggtat ccacgaaatc tacttcccgt
    10501 atgtgctgat gaatccgcgt ctgatcctcg cagtcatcct cggcggtatg actggcgtgt
    10561 tcacgctgac tatcctgggc ggtggtctgg tttctccggc atctccgggt tctatccttg
    10621 ctgtactggc gatgacacca aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg
    10681 cgatggctgt ctccttcgtt gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag
    10741 aagatgatat tgaagcagca actcgtcgta tgcaggacat gaaagctgag tctaaaggca
    10801 catctccgct atctgcgggc gatgtgacta acgacctgag ccacgtacgt aaaatcatcg
    10861 ttgcctgtga cgccggtatg ggttcaagtg cgatgggcgc aggcgtgctg cgtaagaaaa
    10921 ttcaggatgc aggtctgtcg cagatttcgg tcactaacag cgcgatcaac aacctgccgc
    10981 cagatgtgga cctcgtcatc actcaccgtg acctgaccga acgcgctatg cgccaggttc
    11041 cgcaggcaca gcatatttcg ctgaccaact tcctcgacag cggcctgtac accagcctaa
    11101 ccgaacgtct cgttgctgcc cagcgccata ctgaaaacga agtgaaagtg aaagacagtc
    11161 tgaaagacag ctttgacgat tccagtgcta acctgttcaa actgggcgcg gagaacatct
    11221 tcctcggtcg caaagcagca accaaagaag aagcgattcg ttttgctggc gagcagctgg
    11281 tgaaaggcgg ttacgttgag ccggaatacg ttcaggcgat gctggatcgt gaaaaactga
    11341 ccccgactta tctgggtgag tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc
    11401 gcgtactgaa aacgggcgtg gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag
    11461 aagaagatga cattgcccgt ctggtgattg gtattgcagc ccgtaacaac gagcacattc
    11521 aggttatcac cagcctgacc aatgcactgg atgatgagtc cgtcatcgag cgtctggcac
    11581 acaccaccag cgtggatgaa gtgctggaac tgctggcagg tcgtaagtaa tccaatccta
    11641 ccctctcctc acggagaggg tttgggtgag ggaaaagcct caccccagcc ctctcgggta
    11701 aaaacattga tgaaggttaa tactatgaaa gcattacatt ttggcgcagg taatatcggt
    11761 cgtggcttta tcggtaaact gctggcagac gcgggtatcc aactgacgtt tgccgatgtc
    11821 aatcaggtgg tacttgatgc cctgaatgcc cgtcatagct atcaggttca tgtggtcggt
    11881 gaaaccgagc aggtagatac cgtttccggc gtcaatgctg tcagcagcat tggtgatgat
    11941 gtcgttgatc tgattgctca ggtcgattta gtcactaccg ccgttggccc ggttgtgctg
    12001 gaacgtattg ctccggctat cgccaaaggg ctggtgaaac gcaaagaaca aggtaatgaa
    12061 tccccgctga acatcatcgc ctgtgaaaac atggtacgcg gcaccacgca gctgaaaggt
    12121 catgtgatga acgccctgcc ggaagacgcc aaagcgtggg tagaagagca cgttggcttt
    12181 gtcgattccg ccgttgaccg catcgtaccg ccttcggctt cggcaactaa cgatccgctg
    12241 gaagtgacgg tagaaacttt cagcgaatgg attgtcgata aaacgcagtt caaaggcgca
    12301 ctgccgaaca tcccaggcat ggagttaacc gacaacctga tggcatttgt cgaacgtaaa
    12361 ctcttcaccc tgaacacggg tcatgctata accgcgtacc tcggaaaact ggccggtcat
    12421 cagaccattc gtgacgctat tctcgacgag aaaatccgcg cggtggtaaa aggtgcgatg
    12481 gaagaaagtg gtgcggtact gatcaagcgc tacggctttg acgcagacaa acatgcggcg
    12541 tacatccaga aaatcctcgg tcgttttgag aacccgtatc tgaaagatga tgtagagcgc
    12601 gtaggccgtc agccgctgcg taaactgagt gctggcgacc gtctgatcaa gccactgctc
    12661 ggtacgctgg aatacagcct tccgcacaag aatctgattc aggggattgc tggtgcaatg
    12721 cacttccgca gtgaagatga tccgcaggct caggaactgg cagcactgat cgctgacaaa
    12781 ggtccgcagg cggcgctggc acagatttcc ggtcttgatg ccaacagcga ggttgtatcc
    12841 gaggcggtaa ccgcttataa agcaatgcaa taatggtgga ccaggcgcag gacaccctgc
    12901 gcccgaataa cagattgtca gatatgcagg caacaatgga acaaacccag gccgttgaaa
    12961 accgtgtgct tgagcgtctg aatgctggca aaaccgtgcg aagctttctg atcaccgccg
    13021 tcgagctttt gaccgaggcg gtaaatcttc tggtgcttca ggtattccgc aaagacgatt
    13081 acgcggtgaa gtatgctgta gaaccgttac tcgacggcga cggtccgctg ggcgatcttt
    13141 ctgtgcgttt aaaactcatt tacgggttgg gcgtcattaa ccgccaggaa tacgaagatg
    13201 cggaactgct gatggcattg cgtgaagagc taaatcacga cggcaacgag tacgccttta
    13261 ccgacgacga aatccttgga ccctttggtg aactgcactg cgtggcggcg ttaccaccgc
    13321 cgccacagtt tgaaccagca gactccagtt tgtatgcaat gcaaattcag cgctatcaac
    13381 aggctgtgcg atcaacaatg gtcctttcac tgactgagct gatttccaaa atcagcttaa
    13441 aaaaagcctt tcaaaagtaa gcaacgtctg cttactgccc ctctacctgc ttcggccgat
    13501 aaagccgacg ataatactcc agacgttgta gatataacgg cacgctttca tcgggtattc
    13561 cggacggaat cgcgttacgg ggaggaagtt ttttcagata ctcccggaac gcctggcttg
    13621 atgccatgaa atctacggct ttatcgataa gaagcggaac gttttcacct aatttgccca
    13681 tgatgatatc tccgtattac cccgcgccgg gaatgcgcgg ccgccaattt tagtttaggt
    13741 tcccggtaaa gatgacatta aggaaaacgt gctgaatcct caataatcat ccgctgctgt
    13801 ttattatctt tttctataga tttcttaatt aatcaacgaa ttgcgtagtt tttgagcatg
    13861 ttaatgatta cgttgcagaa ctattacaat gtcatcagtt gtgaacaaag cacctggtcg
    13921 cgcatactag gggctataaa tttatcttta tcagaagcca tcacatgaaa gaagtcgaaa
    13981 aaaacgaaat caagcgtctc agcgatcgcc tggacgccat ccgccaccag caggccgatc
    14041 tgtcgctggt tgaagccgca gacaaatatg ccgagctgga aaaagagaaa gccacgctgg
    14101 aagcagaaat tgctcgcctg cgtgaggttc atagccaaaa actgagtaaa gaagcacaga
    14161 agctgatgaa gatgccgttc cagcgcgcaa ttaccaaaaa agagcaggct gatatgggca
    14221 agctgaagaa aagtgttcgc ggactggtgg ttgtgcaccc aatgaccgcg ctggggcgcg
    14281 aaatgggcct ggaagagatg actggatttt ctaagactac gttttaagcc cctcagacga
    14341 ctggttcgat cgtatcaacc cgcatctggc ttgccttagc tggtcaatgc ggggactctc
    14401 ctgtctactc gcctaattac c
//
LOCUS       DAJTSA010000096        14368 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000096 DAJTSA010000000
VERSION     DAJTSA010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.096"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..185
                     /locus_tag="OO369_003174"
     CDS             <1..185
                     /locus_tag="OO369_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCR5983346.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
     gene            complement(210..401)
                     /locus_tag="OO369_003175"
     CDS             complement(210..401)
                     /locus_tag="OO369_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR5983347.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGFWQPAQEYGDF"
     gene            complement(506..742)
                     /locus_tag="OO369_003176"
     CDS             complement(506..742)
                     /locus_tag="OO369_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR5983348.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="OO369_003177"
                     /pseudo
     CDS             complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="OO369_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="OO369_003178"
                     /pseudo
     CDS             complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="OO369_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="OO369_003179"
     CDS             complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="OO369_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR5983349.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            6381..6878
                     /gene="msrC"
                     /locus_tag="OO369_003180"
     CDS             6381..6878
                     /gene="msrC"
                     /locus_tag="OO369_003180"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR5983350.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6975..7673
                     /gene="proQ"
                     /locus_tag="OO369_003181"
     CDS             6975..7673
                     /gene="proQ"
                     /locus_tag="OO369_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR5983351.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7693..9741
                     /gene="prc"
                     /locus_tag="OO369_003182"
     CDS             7693..9741
                     /gene="prc"
                     /locus_tag="OO369_003182"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR5983352.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9933..10814
                     /gene="htpX"
                     /locus_tag="OO369_003183"
     CDS             9933..10814
                     /gene="htpX"
                     /locus_tag="OO369_003183"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR5983353.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10860..12233)
                     /locus_tag="OO369_003184"
     CDS             complement(10860..12233)
                     /locus_tag="OO369_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5983354.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12410..13201
                     /gene="kdgR"
                     /locus_tag="OO369_003185"
     CDS             12410..13201
                     /gene="kdgR"
                     /locus_tag="OO369_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR5983355.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13344..13583)
                     /locus_tag="OO369_003186"
     CDS             complement(13344..13583)
                     /locus_tag="OO369_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983356.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13742..13885
                     /gene="mgrB"
                     /locus_tag="OO369_003187"
     CDS             13742..13885
                     /gene="mgrB"
                     /locus_tag="OO369_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR5983357.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13960..14247
                     /locus_tag="OO369_003188"
     CDS             13960..14247
                     /locus_tag="OO369_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR5983358.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
BASE COUNT         3771 a         3515 c         3686 g         3396 t
ORIGIN      
        1 atgaatggca aaaggacgtt gatttgcatc aggtcttgtg gagccgctcg cgattaggtg
       61 aacgcccaaa agggcaggga attacaggtg ctgatcattt ctggtttggt catacaccgt
      121 tgcgacatcg cgtggatatt ggcaacctgc attatattga taccggtgct gtctttgggg
      181 gctaactgac tcttgtgcaa ctgcaataat taaaaatcac catactcctg tgctggttgc
      241 cagaaaccat ctataaaatc ctcaatcgga aaacaactgc catggcggat ccgttgatcg
      301 ctcatagaat aaagacactg ctgttccgtg ttgtagacat ccacaacaat atcttcacaa
      361 ccgccatcca ggtagcaaac aaaaagtacc agcgcgaaca tttcatcccc gaagtgtggt
      421 gccgtaccgt taagtttagg agagatttta caacggggga ataaccagga caaataaccc
      481 gccatgccga cgggttcttt ttggatcagg caagacgcat aatccaggca tcagattgtt
      541 gatcgtaacg ggtacggtaa aggacagagt gttcgccatt tagcagggca gggatactgg
      601 tttcagcatc ccaggcatcc agttgcatac ataaatcggg gtcagattta caaggaatgg
      661 ttaacgttcg atcacccggc gattcaccgt gtaattcgcc gtcgtcgatt tcaaaagcgc
      721 ctatgcgcac actggttttc atcttcgtgc tctccattta gctgctgatt gtagtacgac
      781 cagtccgatc gcacaatttt tcagcctagt tgaggccggc gaatgtgcca gtcaaaaagt
      841 gcgctttttt tgtgcgctgt catgcgttac tggtaaaaag tttcccgtcc cgcaccagtt
      901 cacgcgggta gctgtttttc agtcgcgaac caatccgttt ggctaaacca atcggctgat
      961 gctggaaagt aaccaacacg tcatccgcca ctggcgcggc ttgcgggtaa acatcgcgcc
     1021 cgcgatacca ctcctacgct tcctgcggtg tcagttcaaa agcgtttaca ttgtcggggg
     1081 aggcatgggc aataaccgct tcatgctgcc agcgataacc tttgttatgc gtttcggcaa
     1141 gtttaatccc taagcgagaa aatcggactt taccgatcag ggcttcaatg cctaccggga
     1201 acaaccacag ttctttgtcg cgttgccaga gtcgcaggtt tccatcccag tttaagccaa
     1261 cacttgcagc cgcctgacga atttgtccgg cttcgcgatc tttcaccggg ctgaacggga
     1321 aattgcccac tttgtatttt ggggcgggta aggcggggat cgcctgagtt ttacgcagac
     1381 gagcaacgaa gaagccttcg cagtcgtaaa tttgtgggaa aacatgcaaa aaaccttctt
     1441 cggtcagcgc cttgtttgca ccagggaaga gatcgccaag tggtaaaaac tctacttcgt
     1501 cggggtaagt ctctttcagc caccggcaaa cggcttcgtt ttcttcccgg tttaaggtat
     1561 aggtcgagta aaccagcgta ccgccaggac gtaatgcatg aaaggcgctg tcaatcagtt
     1621 cccgttgggt agctgcgatt tcctgattgc tttcgggtga ccagtttttt agcgcatcgg
     1681 gatctttacg caccacgcct tcgccggagc agggagcgtc cagcaaaatg gcatcaaaca
     1741 tttctggcac tgccgcacca aacacgcggc caccaaaatg tgtgagcgca acattactga
     1801 tgccacagcg gctgatattg gcatgtaaca cttttaccca actggcggaa aactcattgg
     1861 caaggattgc cccttcgtta ttcatccgcg cggcaatttg cgtcgttttt gagcctggcg
     1921 cagcagcgac atccatcacc cgctgtggtg cattaccgtc agcaaacaag gcggcgacgg
     1981 gcaacattga actggcttcc tgaatataaa acaggccact taaatgctcg gtggtactac
     2041 ccaatggcaa tgcatcttca ttgtcgcgtt caatccagaa accttcttca caccacggaa
     2101 ttggcgtaag cgtccagcca taaggagcgg ttaattgcag gaaatcagca acggagattt
     2161 tcagcgtatt aacgcgaatg ctgcggcgca acgggcgctg acaggcggca agaaaatcat
     2221 caaatgagag cgtcgaaggc atagcttcgc gcatttgtgt cagaaacgcg tccgggaaat
     2281 aaacggtgtg ttgggccacg agcatgtacc actggcaaaa aaacaggcgc gcagtttagc
     2341 ataagcgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagttccc
     2401 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgtttgcc ttcttgggct
     2461 ttcggtgcaa gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaattgg
     2521 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     2581 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     2641 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2701 agaccacgga acagcacagg cgtaccgatg cctaacgaac cggcttccgg cgcttcaaca
     2761 ataatgctta aaccatctag gtaacgcgaa tcagtaatgg tggcctcttc taattcaaag
     2821 tcgcgacgag gattgccacg gcctggttcg acgttgatat acggctggag gatagtatct
     2881 agatgctcaa cgccagctgc cgagatttgc ggtgtgacca ctgagaagcg cgtaccaccg
     2941 cgagcaaagg tctggacata ttccggatag agcaccgcct ttgcctgtac ttcattgcgt
     3001 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     3061 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgcct gccaaccgca
     3121 cgggccgctg tttcggaagc atacagaatt cgcttgtcgc ctttacgctg actggcgctg
     3181 gcaccactga ggttatcgaa gctaatggcc ccctttaatg ctctggagag cggggatgcc
     3241 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     3301 ctgttgctgg tcaaaaggtt gcgatattcc ggtttaatgt gcagatcgat atcgaacgcg
     3361 ttagctcgcg gacgcacggt aataacttca ccaacttcaa atttacggta cagcactacc
     3421 gatcctgcct gcacatccgg cagcgtctct gcactcaaac tcatggttgt ggtgggtaaa
     3481 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     3541 atctcgcctt tatcgcccgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     3601 gcaccgagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3661 aatttgctat cgccttttac cagttctcga tgctgaggct cgatggcgac ggtaaaggtg
     3721 acgcctttgc tggtgagttt acgatcgata acctggccta cctgcacgcc gtgaagaatg
     3781 agcggctgac ccgcatcaat accgtaactt tccggtgctg tcagggtcag tgtcagaacg
     3841 tccggctctt gcagcagggc tttttcgccc ggcacaacaa cgaactcttt gcgtggctcg
     3901 ccatcgccgg gcaccaactc aaaggttttt ccggtcagca gggcgctgag attagcatcg
     3961 ctaagggata atttcgggtt gcgtaattcg atgcgggtat tttcacgaag cagggatcaa
     4021 cggtcatttc cccagtgact ttaccaccag gatttaaatc cagtttagtc agctgtccga
     4081 cttccagccc ctgatacatt aacggcgtcg agtcggcggt taatcctgct ccactcggca
     4141 gttccagttt tattattact ccacgctggc tgtgggccag atcttcatac agaccaaagg
     4201 tatcttccgc ctcggcaggt ttcgactctt caggtgaatc gaaggcaatc gcaccgttaa
     4261 ccagtgccgc cagactttcc agtttcacct tcgcgccact gatactgacg ttggcatcaa
     4321 cgccggaaac gtttcagaaa cggctacctt ttttcaccag atcggtaaaa cgccgctcga
     4381 tcaggacatc aatcaccacg ccttgcttgt tgggattgat ggcatagtcg tagacttttc
     4441 ccaccgggat cttgcggaaa tagaccaatg aaccgctgtt cagcgaaccg agagcggggg
     4501 cttgcaggtg gatcatcaga tcgccattgt ccagccgata tttcggttgg gtatcgagcg
     4561 cgacaaagtg atcctgctct ttacctttac ccggcatcat gccgatatag ttcccaccga
     4621 cgagggcgtc cagcccggag acacctgcca acgatgcttt tggcgtcatc agccagaact
     4681 gagtctcttc gcgcagcgca tctttcatat cggacttgat gctgaccttg acttcaatct
     4741 tacgaagatc gtcgctgagg ctgatatcct gcactgttcc gacttcaacg ccctgataac
     4801 gaacaggcgt acggcccgga acaataccat ccgccgacat aaagtcgatg gtgacggtat
     4861 taccccggtc ctgataactg tcccaaatca gccaaccggc aatcattagc gcgatgaaag
     4921 gcagcagcca gaaaggtgag atacggcgtt tatttttaat ctgcgcttca gtcgtcgaag
     4981 cgggcgtttc ctgactcatg tgcatcccaa agtaagcggc tgtccagcca ttccacagca
     5041 agaatagtca aaattacggc agcgccaaaa taaaacgcag ccggtcccat agtaaaagcg
     5101 aggatctgat cgcgattaat cagcgacatg gttaaagata tgacaaacag gtcgagtatc
     5161 gaccagcgtc caatccaggt caccatccgc agtaacagaa tgcgtgtgcg taaaccttgc
     5221 tggcatttaa aatgaatgct caacagtaaa gtgaacatga cgatcacttt agtaaacggt
     5281 accagaatac tggcgataaa cacgattccc gcaaccgcaa tgttgctact tgccagcgac
     5341 ataattccgg aaagaatcgt atcttcctgc cggccaccat tcagataaat gatagaaata
     5401 ggcaataggt tggctggtaa caacaaaacg attgacgcta acagcgccgc ccagcatttt
     5461 tgcaaactat gacgacgacg caggcgtagc gggatatggc aacgcgggca gagaccacgc
     5521 tgatcaggat agccggtaaa atggcaccca agacagacac gaagtttctc gtccctacgc
     5581 gtagcggggc gctgcggata atatcgctcc cacagctcct caacattaag atgtgacaac
     5641 gtcaccgtcg ttaaaatcac caacgccaca aaagagaaca agccgacacc cgcctggata
     5701 tgggcataat cctgtacctt tatagaagca acgccaatgc cgaccaggta gatatccagc
     5761 attacccact ctttaagtcg ctcaagcatc agcagtaccg gacgtaaatt cattcccagt
     5821 cggttaccaa accataaata tgctatggag gacaccagaa tgaggggggc accaataacg
     5881 cagaaaaaga ccattgatcc cgttatcgca tcgccctgtt tggtcatttg ccagatgcct
     5941 tgcatgacgt tggcgtcaat acgaatgcct aacagccaga tatgcaacag cggttcgccc
     6001 caggcaaacg gcataagcaa cagcatggtg aaggccattg ccgccaggcg cgttagcgac
     6061 cagtcgcgcc cgtcgcgaat ttttgcctga cagcgcggac aataggcact ttgatgagaa
     6121 tttatctcgg gcaggctaaa cagcatgtca cattgcgggc aacgttggta atcaccacgc
     6181 ggcagttcct cgccgattgc cctcactgtt atcttttttg tcggcgtaat ttgtggtgtg
     6241 ttaagagcca tgacgtgcca aatgtaataa gaattaacat taatcttaac ccatgatggg
     6301 aatgatcttg agcattaacg catttaatgc ttattttaat aggctggcag ttccaggtaa
     6361 gacaaccaag tgattaagta atgaacaaaa cagaatttta cgcggattta aatcgcgact
     6421 ttaacgcgct gatggcggga gaaaccagtt ttctggcaac gcttgcgaac accagtgcgt
     6481 tgttatacga gcgtctcact gacgttaact gggcaggttt ttatttgctt gaggacgata
     6541 cactggtact cggaccattt cagggcaaaa ttgcctgtgt ccggataccc gtcgggcgcg
     6601 gggtgtgcgg cacagcggtt gcccgcaatc aagtgcagcg tatcgaggat gttcacgcgt
     6661 ttgacgggca tattgcctgt gatgcggcga gtaattctga aattgttctg ccgctggtgg
     6721 tgaaaaatca gattattggt gttctcgaca tcgatagtac tgtcttcgct cgctttacag
     6781 acgaggacga gcaaggctta cgtcagcttg tggcacagct tgaaaaagtg cttgcaacga
     6841 cggattacaa aaaattcttt gcgagcgtcg caggataatc aacggataac gtagcattta
     6901 ctggtagcgt cattataatg acgcctgttc atgcctgcgc ttgttggcta cgtccgttgt
     6961 aatcaggaaa tttcatggaa aatcaaccta agttgaatag cagtaaagaa gtaatcgcgt
     7021 ttctggccga acgttttccc cactgtttca gtgcggaagg tgaagcgcgt ccgctgaaaa
     7081 ttggtatttt tcaggatttg gtcgatcgtg ttgccgggga aatgaacctg agcaaaacgc
     7141 aattacgatc cgctttacgt ctctacactt cgagctggcg ttatctttac ggtgttaaac
     7201 ccggcgcaac gcgtgtcgat cttgacggca acccatgcgg tgagctggac gagcaacatg
     7261 tagagcatgc tcgcaagcag cttgaagaag cgaaagcgcg tgttcaggca cagcgtgctg
     7321 aacagcaagc gaaaaaacgc gaagctgccg cagctgctgg tgagaaagaa gacgcaccgc
     7381 gccgcgaacg caagccacgt ccgaccacgc cacgccgcaa agaaggcgct gaacgtaaac
     7441 ctcgtgcgca aaagccggta gagaaagcgc caaaaacagt aaaagcacct cgcgaagaac
     7501 agcacacccc ggtttctgac atttcagctc tgactgtcgg acaagccctg aaggtgaaag
     7561 cgggtcaaaa cgcgatggat gccaccgtat tagaaatcac caaagacggc gtccgcgtcc
     7621 agctgaattc gggtatgtct ttgattgtgc gcgcagaaca cctggtgttc tgaaacggag
     7681 gccgggccag gcatgaacat gttttttagg cttaccgcgt tagctggcct gcttgcaata
     7741 gcaggccaga ccttcgctgt agaagatatc acgcgtgctg atcaaattcc ggtattaaag
     7801 gaagagacgc agcatgcgac ggtaagtgag cgcgtaacgt cgcgcttcac ccgttctcat
     7861 tatcgccagt tcgacctcga tcaggcattt tcggccaaaa tctttgaccg ctacctgaat
     7921 ctgctcgatt acagccacaa tgtgctgctg gcaagcgatg ttgaacagtt cgcgaaaaag
     7981 aaaaccgagt taggcgatga actgcgttca ggcaaactcg acgttttcta cgatctctac
     8041 aatctggcgc aaaagcgccg ttttgaacgt taccagtacg ctttgtcggt actggaaaag
     8101 ccgatggatt tcaccggcaa cgacacttat aaccttgacc gcagcaaagc gccctggccg
     8161 aaaaacgagg ctgagttgaa cgcgcagtgg gacagtaaag tcaaattcga cgagttaagc
     8221 ctgaagctga caggaaaaac ggataaagaa attcgtgaaa ccctgacacg ccgctacaaa
     8281 tttgccattc gtcgtctggc gcaaaccaac agcgaagatg ttttctcgct ggcaatgacg
     8341 gcgtttgcgc gtgaaatcga tccgcatacc aactatcttt ccccgcgtaa taccgaacag
     8401 ttcaacactg aaatgagttt gtcactggaa ggtattggcg cagtgctgca aatggatgat
     8461 gactacaccg ttatcaattc gatggtggca ggtggtccgg cagcgaagag taaagctatc
     8521 agcgttggtg acaaaattgt cggtgttggt caaacaggca agccgatggt tgacgtgatt
     8581 ggctggcgtc ttgatgatgt ggttgcctta attaaagggc cgaagggcag taaagttcgt
     8641 ctggaaattt tacctgctgg taaagggacc aagacccgta ctgtaacgtt gacccgtgaa
     8701 cgtattcgtc tcgaagaccg cgcggttaaa atgtcggtga agaccgtcgg taaagagaaa
     8761 gtcggcgtgc tggatattcc gggcttctat gtgggtttga cagacgatgt caaagttcaa
     8821 ctgcagaaac tggaaaaaca gaatgtcagc agtgtgatca tcgacctgcg tagcaatggc
     8881 ggtggggcgt tgaccgaagc ggtatcgctc tcaggtctgt ttattcctgc gggtcccatt
     8941 gttcaggtcc gcgataacaa cggcaaggtt cgtgaagaca gcgataccga cgggcaggtg
     9001 ttctataaag gcccgctggt ggtactggtt gaccgtttca gtgcttcggc ttcagaaatc
     9061 tttgccgcgg caatgcagga ttacggtcgt gcgctggttg tgggtgaacc gacgtttggt
     9121 aaaggcaccg ttcagcaata ccgttcattg aaccgtattt acgatcagat gttacgtcct
     9181 gaatggccag cgctgggttc tgtgcagtac acgatccaga aattctatcg cgttaacggc
     9241 ggcagtacgc aacgtaaagg cgtaacacca gacatcatca tgccgacggg taatgaagaa
     9301 acggaaacgg gtgagaaatt cgaagataac gcgctgccgt gggatagcat cgatgccgcg
     9361 acttatgtga aatcaggaga tttaactgcc tttgaaccgg agctgctgaa ggaacataat
     9421 gcgcgtatcg cgaaagatcc tgagttccag aacatcatga aggatatcgc gcgcttcaac
     9481 gctatgaagg acaagcgcaa tatcgtttct ctgaactacg ctgtgcgtga gaaagagaat
     9541 aatgaagatg atgcgacgcg tctggcgcgt ttgaacgaac gctttaaacg agaaggtaaa
     9601 ccggagttga agaagctgga tgatctatcg aaagattacc aggagccgga tccttatctg
     9661 gatgagacgg tgaatatcgc actcgatctg gcgaagcttg aaaaagccag acccgcggaa
     9721 caacccgctc ccgtcaagta atatcaatca ggcacaagaa attgtgcctg attttttaac
     9781 agcggcaaga tgccgtaaat cagatgctac aaaatgtaaa gttgtgtctt tctggtgact
     9841 tacgcactat ccagacttga aaatagtcgc gtaacccata cgatgtgggt atcgcatatt
     9901 gcgttttgtt aaactgaggt aaaaagaaaa ttatgatgcg aatcgcgctc ttcctgctaa
     9961 cgaacctggc cgtaatggtc gttttcgggc tggtactgag cctgacaggg atacagtcga
    10021 gcagcgttca ggggctgatg atcatggcct tgctgttcgg ttttggtggt tccttcgttt
    10081 cgcttctgat gtccaaatgg atggcattac gatctgttgg cggggaagtg atcgagcaac
    10141 cgcgtaacga aagggaacgt tggctggtca atactgtagc aacccaggct cgtcaggcgg
    10201 ggatcgctat gccgcaagtg gctatctacc atgcgccgga catcaacgct tttgcaaccg
    10261 gtgcgcgccg tgatgcctct ctggttgctg tcagcaccgg tttgctgcag aacatgagcc
    10321 cggatgaagc cgaggcggta attgctcacg aaatcagcca catcgccaat ggtgatatgg
    10381 tcaccatgac gctgattcag ggcgtggtga acaccttcgt tatctttatt tcccgtattc
    10441 tggcgcagct tgccgcgggt tttatgggcg gaaatcgtga tgaaggtgaa gagagcaacg
    10501 gcaacccgct gatctacttt gcggttgcaa cggttctgga actggtgttt ggtattctgg
    10561 cgagcattat caccatgtgg ttctcgcgtc atcgtgaatt ccatgcagat gccggttcgg
    10621 caaaactggt tggtcgcgag aaaatgattg ccgcgctgca gcgcctgaaa accagctatg
    10681 aaccgcaaga agcaaccagc atgatggctt tctgcattaa cggtaagtcg aaatcgctca
    10741 gtgagttgtt catgactcac ccgccgctgg ataaacgtat tgaagctctg cgtacgggtg
    10801 aatacctgaa gtaatatgaa gtaaatacta aaaagcgcgt cctgatcgac gcgctttttt
    10861 tatgccctgg ctcgtggctg agtgatacgt aaaccactga cacaggcagc aatcactgcc
    10921 agaatagccg cagccatcag cgagacgtgc gtaccattat caccaaactg atttagcatc
    10981 agcgccacca gcgccgcgcc actactctga cccagtagac gagccgttcc taacatgcca
    11041 ctggccccac cgctacgttc gcgcggagcg gaggtaataa tggtgtggtt attgggtgac
    11101 tggaataagc caaatccagc gccacataag atcatcggcc agataatatt gatatccgca
    11161 ggtgacgcgg gcagcagaac cagggaaaaa agccccgtag ccatgatgaa caaccctaaa
    11221 gcacccagca atcctgcatg tacgcgttca atcaaatagc ctgccagagg agccatcacc
    11281 atcgttgcta acggccacgg tgtcagaagt aaacctgttt cgacttcact acgcccgagc
    11341 acggtttgca ggtaaaaggg cagggaaacc attgccagca tttgtgcgca gaaagagcaa
    11401 acagatgtgc aaatagaaag tgaaaacagc gggatacgca gtaaatccac cggtagcagc
    11461 ggtacgggaa gagaaagctg gcggcgaata aagaaaatac caacaacaac cattaccacc
    11521 agttccgcag caattaacgt cagcgattgc ccctgagcga aaccactcaa cgcagtgata
    11581 agcaggccga aggttaacgc gttcatcacg gcgctgggca ggtcgaaacg gggtttactg
    11641 gcgcgagaac cattgggtgg cagaaaacgc atcgccagaa gcagggcgat aatacctaac
    11701 ggtacgttga ttaaaaataa ccatttccag gatgcgatgg agaggattgc tgcagcaatt
    11761 gtcggcccgg cagcagaaga gacggcaaca ataaacgagt ttatgcccat ccctctcccc
    11821 agaaaacgtt gtggatagat caggcggata agtgcggtat taacgctcat caacgctgca
    11881 ccgccgaaac cttgtatgac acgcgcaagg gtgagcattt gcagcgaatc agaaagggcg
    11941 cagaacagtg aagacaacag aaaaacgacc agaccgcatt tataaatacg tcgatagcca
    12001 aacatatcgc ccagaaacga aaacgagagc agggagatga caatggcgat ttgataggcg
    12061 ttcactaccc agatggaact ggctggtgtg gcatgaaggt ccgtggcaat tgttggcagg
    12121 gcgacgttgg cgattgcgcc gtcaaggaca gccatcgaaa taccaatcac aatggttaat
    12181 atcgcaccgt atcgctgggg caatggcagg ccgtcggcct gaacttttgg cataggaatt
    12241 ttatatcttt ggtgaataat caacaggggt atgattctag cactgatatt cacggtggat
    12301 gtcgcagaat tgtaactaac cggcaaaaga tggattgcga ccaccctggt gcgaaattat
    12361 aataaaaaac agttctgatt tttataaaac actcgcaatg aggtgataaa tggctaacgc
    12421 agatctggat aaacagcctg attctgtatc ttccgtgcta aaagtttttg gcattttgca
    12481 ggcgctgggt gaagagcgcg aaatagggat aaccgagctg tcgcagcgcg tcatgatgtc
    12541 aaaaagcacc gtttatcgct ttttacagac catgaaaacc ttaggttatg tggcgcagga
    12601 aggggagtcg gagaaatatt ccctgaccct gaaattgttt gaactgggcg ctcgcgcgtt
    12661 acaaaacgtc gatttaattc gtagcgcaga tatccagatg cgtgagatct cccgcctgac
    12721 caaagaaact atccacctcg gcgcactgga cgaagacagt attgtttaca ttcacaaaat
    12781 tgactctatg tacaatttgc gcatgtattc acggattggg cgtcgtaatc cgctgtacag
    12841 caccgcgatt ggtaaggtac tgctggcatg gcgcgatcgc gatgaagtga agcaaattct
    12901 tgagggcgtg gagtataaac gcagtaccga gcggaccatc accagtacag aagcgttatt
    12961 acccgttctg gaccaggtgc gcgagcaggg gtatggagaa gataacgaag agcaggaaga
    13021 agggctgcaa tgcattgcgg taccggtatt tgatcgcttt ggcgtggtca ttgccggttt
    13081 gagcatctcc ttcccgacgt tgcgtttctc tgaagagcgt ttacaggaat atgtcgcaat
    13141 gttgcatacc gcagcgcgca aaatttctgc ccaaatgggt tatcacgact atccgttctg
    13201 atgagagtaa gaacctgtcg gaatatcaaa cagacaggtt ctttatttag catgagaaaa
    13261 ataaagttga aggtgcgtta tattaaacgc gcttgctata agaatatttt actcatgagt
    13321 gagaatctgg ttatttatta cccttaacca ttatcgacca cgatattgct tttacgtaac
    13381 agtgggcaat ctgttatctc caagaaacca cttttagtgt gcaagtattg taccgtgctg
    13441 gtgcctctgg cagtcagata ggtacattgc aaacctaatc ctgcggcatt ctctttgctt
    13501 ccaatcaaaa caccatatcc gctgagtaat aatcctatcc ataccagtgc tatcagcata
    13561 actgtgcgaa tgatgaatcg cattacaatc tcttctcttt ttatgttcgc ttaatcgtag
    13621 cggcaatatg cgctgaagca agctactcat tccgaaaaag cacgaatatc gacatagtta
    13681 ggcgctgttt aactaacgca tgctagttta atgacataag gtaggtgaag cggagattgg
    13741 agtgaaaaag tttcgatggg tcgttctggt tgtcgtggtg ttggcttgct tgctgctttg
    13801 ggcgcaggta ttcaacatga tgtgcgatca ggatgtacaa tttttcagcg gaatttgtgc
    13861 cattaaccag tttatcccgt ggtgatatca ttttttcagc actagatttt cttcctctcc
    13921 agcagtggta gaatagcgcc tctactcaga ggtggtgaaa tgaacgaagt tgtaaattca
    13981 ggcgtgatga acattgcgtc tttggttgta tcggtggtgg ttcttcttat cgggctcatc
    14041 ttgtggtttt ttatcaatcg tgccagttca cggactaacg aacagattga actgcttgag
    14101 gcgttgctgg atcagcaaaa acgtcaaaat gcactgttac gtcgtttgtg cgaagcaaac
    14161 gaaccagaga aagcagataa aaagaccatt gagagtcaaa aatcggttga tgacgaagat
    14221 attattcgcc tggtcgccga acgataatcc ttcctcaatc ttgagtaacc ccgtcgttga
    14281 agggttactc aattcatcct gattatacgc actgtcatgg tgcaactgct tcaaattaaa
    14341 actctggtct gacttcaaat aattatca
//
LOCUS       DAJTSA010000097        14318 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000097 DAJTSA010000000
VERSION     DAJTSA010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.097"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..3051
                     /locus_tag="OO369_003189"
                     /pseudo
     CDS             1..3051
                     /locus_tag="OO369_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3061..6707
                     /locus_tag="OO369_003190"
                     /pseudo
     CDS             3061..6707
                     /locus_tag="OO369_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6768..7175
                     /locus_tag="OO369_003191"
     CDS             6768..7175
                     /locus_tag="OO369_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983359.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7717..8805
                     /locus_tag="OO369_003192"
     CDS             7717..8805
                     /locus_tag="OO369_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR5983360.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8816..11095
                     /locus_tag="OO369_003193"
     CDS             8816..11095
                     /locus_tag="OO369_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983361.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVATYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            11088..12224
                     /locus_tag="OO369_003194"
     CDS             11088..12224
                     /locus_tag="OO369_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR5983362.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            12221..14224
                     /locus_tag="OO369_003195"
     CDS             12221..14224
                     /locus_tag="OO369_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983363.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3547 a         3756 c         3655 g         3360 t
ORIGIN      
        1 gggattatgc gaataatgtc attattattc tgccgtctgg cattgcacct gactacttgg
       61 agcaatattc cagatttgaa ttacgcctac gtaaacattt atcactggcg gaagagtctc
      121 tctggcaaaa atgtgcacaa aaacttattg ccgcaattcc acatattcca gaatggcggc
      181 aaccattaat tgctttgtta ttacccgaaa aaccagaaat tgcacatgaa attgcccagc
      241 atctactggg gcaaaaaaaa ttaccctcgc ttgagtggtt aaaaatagtg gcgactgatg
      301 agcacattct tgcctcatta gaaaaatatc acgagccata tgccattttt gatgattact
      361 attgtggtgc gatatggtca gccaccgtat tacaggagca aggtgttgca gccctgcccc
      421 gatttgctcc ctatgccgca agtgactact gcgccgatgt gttgcgtcat atcaatcatc
      481 cgttcgcatt aacactgctt atacgtgtag ccgggcatac taaacgctgt cacgatcgaa
      541 tgacgaaagc ctgcgctgcg ttcccacacg cagcactggc agcactggcg gaacttctag
      601 tgcaaaaaga agagaatagt tggcgcatta tgctaatgac gatgtttatc tcgcaaccag
      661 cactggcaga acaggtcatt ccctggcttt cgacacccgc agttgcagtg ctgaaatcat
      721 gccagcacca actgacacag ccctcaaacc atgccagcgc cgatctactg ccagccatag
      781 tagtctcccc accatggatt tcgaaaaaga aaaaatcgcc gattccggtg ctggatttag
      841 cgccattagg catcgagcca atctgttatc tgacagaaga aatcagtaat caacttttgg
      901 cgaaatatat ctggtattca aaacacatca cggttagcca tgaagaaagt actaccaacc
      961 tgttggcaag gatgggtttt caacgacgga tcgctggtac atatattaaa gctcccgaag
     1021 cggtagttga ggcatggcta aatgaagatt attcaacctt actaagtgaa tttaaggtgt
     1081 ttcattcacc taccgggcat tattggcagt tggggatttt gacaacattg ccgctggagg
     1141 ctggagaaag cagtaaaagc atggaatgcc cttactctat ctccacatac cgataccgaa
     1201 tacgccatgt tacattttgg actcaaaggt ttacctggtt tagttaactc aattacacgc
     1261 tatccacagg aagccttgcc catcacgaat tacttcgcag cgagtgagct ggctcctgcc
     1321 gtcgcccgtg ccttcaacaa actcaaaacg ctacgcgaaa acgcccgcac ctggctgttg
     1381 aaatacccgg aacatgccct taccggcctg ctgcctgcgg cgctcggcaa agccggtgaa
     1441 gcacaggata acgcccgcgc tgccttgcgt atgcttaccg aaaacggtca tcagccatta
     1501 ctgcaagaaa tcgcccggcg ttataaccag tcggaagtaa cagacgcggt gaacgctctg
     1561 cttgcgctcg atcccttaga taatcacccg acaaaaatcc ccactcttcc gaccttttat
     1621 cagccatcga tctggacgcg cccggtatta aaagcaaacg cccaatcact gccagataac
     1681 gccctcctcc acctcggcga aatgctccgc ttccctcagg aagaggctct gtatccggga
     1741 ttattgcagg tgaaagacgc ctgtaccgct gactctctgg ctgaatttgc ctgggatctg
     1801 tttaccgcct ggcagaccgc tggcgcgccg tcgaaagaga gttgggcgtt cactgcgtta
     1861 ggcgttctcg gtaacgatga caccgcccgc aaactgacgc cattaatacg cgcctggcct
     1921 ggtgaatccc agcataaacg cgccaccgtt gggttggata ttctcgccgc tatcggtagt
     1981 gatatcgccc tgatgcagct taacggcatt gctcagaaac tgaaattcaa agcattacag
     2041 gagcgggcaa aagaaaagat tgccgacatt gccgagagcc gcgaactcac ggtagcggag
     2101 cttgaagatc ggttagcacc ggatctcggt ctggatgata acggttcgtt gctgctggat
     2161 tttggcccac gccagttcac cgtcagcttt gatgaaacct taaaaccgtt tgtgcgtgat
     2221 gtttccggca gccgcctgaa agacctgcca aaaccaaaca aaagcgatta tgagtcacag
     2281 gcgaacgatg ctgttaaccg ctacaaattg ctgaaaaaag atgcgcgtac cgtcgcggcc
     2341 cagcaggtag caaggcttga atccgccatg tgcctgcgtc gccgctggtc gccggaaaac
     2401 ttccaactct tcctggttgt gcatccgctg gttcgccact taacccgccg tctgatttgg
     2461 ggcgtttata gcgccgacaa ccagctactg gcctgtttcc gggtagcgga agataacagc
     2521 tacagcaccg ctgacgatga tcttttcacc ctgccggaag gcgatatctc tatcggcatt
     2581 cctcacgttc tggaaatatc accgacggat gctgtcgcct ttggtcagct ttttgccgac
     2641 tacgaactgc taccaccgtt ccgtcagctt gaccgtaaca gttacgccct gacagaagcc
     2701 gagcgcaacg ccagcgaact gacccgctgg gcaggcagaa aatgcccgag cggccgggtc
     2761 atggggctgg caaataaagg ctggataaag ggcgaaccac aggatggagg ctggatgatc
     2821 aaacctctcg gtcgctggtc gttaatcatg gaaatcgacg aaggttttgc ggtgggtata
     2881 tcaccagccg aacttagcgc tgagcagctc ttaagcaagc tgtggctatg ggaaggcaaa
     2941 gcggaaagct atggctgggg gagtaattca acacaggaag cgcagttctc cgtactcgat
     3001 gccatcaccg ccagcgagct aattaacgat attgaagccc tgtttgaata aggaaagagc
     3061 atggacaagg aattaccgtg gctggcggat aacgcccaac tggaactgaa atataaaaaa
     3121 ggcaaaacac cactcagtca tcgcaattgg ccgggcgaac cagtgcctgt tatcactgaa
     3181 agcatcattc agacactggg tgatgaattg ctacaaaaag ctgagaagaa aaaaaacatt
     3241 gtctggcgtt atgaaaattt ttcaccggag tggcagtccg ccattacgca ggccatcaac
     3301 ttgatcggcg aacacaaacc ctcagtcccg gcccgcacaa tggcggcact agcctttatc
     3361 gcgcaaaatg acaaccaaca gttgctcgac gaaatcgtcc aacaagaggg gctggaatat
     3421 gcgactgagg tggtgattgc acgccagttt attgcacggt gttatgagag tgatcctctg
     3481 gtagtcacat tgcagtatca gaacgaggac tatggctatg gttatcgctc agaaacctat
     3541 aacgaattcg atctccgact gcgtaaacac ctctctctgg cagaggaaag ctgctggcag
     3601 cgttgcgccg acaaactcat tgccgcactg ccaggaataa ccaaagttcg ccgccctttt
     3661 attgcgctga tcctccccga aaaaccagaa atcgccaatg agttggtaag ccttgaatgc
     3721 ccgcggactc attttcattc taaggagtgg ttaaaagttg ttgctactga ccccaaagcg
     3781 gtgagaaaac tcgaacgcta ctggagccag gatatattta gcgatcgaga agccagctac
     3841 atgtcgcatg aaaaccactt cggctacgcg gcctgcgctg cccttttgcg cgaacaagga
     3901 ctggcagtcg ttccgcgcct cgcgatatat gcccataaag aagattgcgg cagtctgctg
     3961 gtacaaatta accatccgca agtcatccgc accttgctgt tagttgctga taaaaacaaa
     4021 cccagcctgc aacgtgtggc taaatacagt aaaaacttcc cccatgcgac actcgccgca
     4081 ctggcagaac tgctggcgtt aaaagaacca ccagcccgcc caggttatcc aatcatcgaa
     4141 gacaaaaagc tgcctgcaca gcaaaaagct cgagatgaat actggcgtac gctgttacaa
     4201 acgctgatgg catcgcagcc acaactggca gcagaagtga tgccgtggtt aagtactcaa
     4261 gcccgggcag tgctgaatag ttatttatcg gcaccgccca aaccagttat tgatagcacc
     4321 gataacagcc aaatgcctga aatcctcgtt tcaccaccat ggcgtgggag aaagaaaaag
     4381 acttccatac gtcacttttc gctgcctgat cttacgcttg caccgcaaac gtacaggtcg
     4441 ccgaataaac actggacgcc ggaacagcaa actgcaattg accattttag cgccatgtct
     4501 tttgacgaac gtcttgctac gagaggagcg gatatcttct tgcgggaatt aggttttgac
     4561 gttcatagcc tggaatttaa taagcgtctt cttaatggac agttggatgc ctttgatgct
     4621 cagcaactgc aagaatacac atcaatagga ctattgcgta cttatcacgc gcatcaggcc
     4681 aaatctaaac agtaccggga agacgccacc aatgcattac tggcacaaga tagcattgcg
     4741 ttgatgggag ggtggcgaac ttttcagcac ggattttact atggtaatga acagcgttgg
     4801 gggatttgga atctgtacct tatcgcgcag atgccgcgcg aaatggcggt atcgtgctgg
     4861 caacaaattg tcgaggctga ttttcattac accggggtgg aatatttgtt aagtgtgctg
     4921 ggtaccgatg ctttgcctgg attaaacacc gcctttgccc gacatccaaa agagatattt
     4981 ccgctgctga ttcatttcgg cgcaacagaa ctggccctgc ccattgcccg cgtctggcac
     5041 ctttttgcgg gccagcgtaa tctggctcgc cagtggattt tacaatggcc ggaacatacg
     5101 gctactgcac ttatccctct tgtcttcacc aaatctagcg ataaaagcga agctgcatta
     5161 cttgccctgc gtttacttta cgaacatggg catggcgaat ggctacaaac tgtagccaat
     5221 cgttggcagc gtacagatct atggcctgcc ctggaacaat tacttaaaca gagtccaata
     5281 gagatttacc cggcacgcat tccaaaagcc cctgatttct ggcatccgca aatgtggtcc
     5341 aggccgcgcc ttatcactaa taatcaacct gttaccgatg acgctctgga aattatcggc
     5401 gaaatgctgc gctttaccca ggggggacgt ttttatagcg ggctggaaca actgaaaacg
     5461 ttctgccagc cacaaacgct ggcagctttt gcctgggatc tcttcactgc gtggcaacaa
     5521 gctggtgccc ccgcaaaaga caactgggca tttctggcgt taagtctctt tggtgacgaa
     5581 agcacggcac gggatctaac gacacatatc ctcgcctggc cacaagaagg caaatctgcc
     5641 cgtgcggtca gtggcatgaa catccttacc ctgatgaata atgatatggc gctgatgcag
     5701 ctgcatcata tatcgcaacg ggcgaaatcc cgccccttac gtgataacgc ggcggaattt
     5761 cttcaggtag tcgcagaaaa tcgcgggcta agccaggaag agttagcgga cagattagtc
     5821 ccaacactgg gccttaatga tccgcaggcg ttgagttttg attttggtcc ccggcagttt
     5881 accgttcgct tcgatgaaaa cctcaacccg gttatctttg atcagcaaaa cgttcgccag
     5941 aaaagtgttc cccggttgcg cgccgatgac gatcaactga aagcgcccga ggcactggcc
     6001 cgactaaaag ggttaaaaaa agatgctact caggtgagca aaaacctgct cccgcgtctt
     6061 gaagctgccc tacgtaccac ccgacgctgg tcgctggcag attttcattc tctgtttgtt
     6121 aatcatccct ttacccgtct ggttacccag cgattaatat ggggggttat ccggcaaatg
     6181 aaccgcgtcg tttactcaac gcctttcgtg tggccgcaga gggggagttc tgcaatgcgc
     6241 aagatgagcc aattgacctg cctgcggacg ctctgattgg cattgcccac ccgttagaaa
     6301 tgacagcaga aatgcgcagt gaatttgcac agctttttgc cgattacgaa attatgccgc
     6361 cttttcgcca gttgtcgcgc cgcacggtgc tgctcacacc tgacgagtca accagtaaca
     6421 gcctgactcg ctgggaaggt aaatccgcta ccgttgggca acttatggga atgcgataca
     6481 aaggctggga gtcaggttat gaggacgcat ttgtctatga cctgggtgag taccggctgg
     6541 tccttaagtt ttcacccggt tttaaccact acaatgttga tagcaaagcg ctaatgagct
     6601 tccgttctct tcgagtgtac cgtgacaata aatccgtcac ttttgccgaa cttgatgtgt
     6661 ttgatttgag tgaggcgtta agcgcacctg acgtcatttt ccattaaaac aaagcgggca
     6721 gtgatgcgac tgcccgttat ctacacgact taccagcggg gaaagcgatg atcgtgcaaa
     6781 aagagctggt tgctatttac gattatgagg tccctgtacc tgaagatccg ttttccttca
     6841 gacttgagat ccataaatgc tctgaattat ttacaggttc cgtctaccga ctggaacggt
     6901 tccggctacg tccaacattt catcaacgtg atcgagagga tgttgacccg ctaataaatg
     6961 atgcgttgat ttatataaga gatgagttta ttgatgagca aaaattacga ggcgaatcac
     7021 ctgaacctgt aatagtaatt tttaattgta aactacagaa tatattcaac caacaaatag
     7081 aattatatac tctagataat tcaagtcgca gcacatgcaa cttgaagtat gacgagtata
     7141 gctttattaa aggggggaac ggtattcccc cttagatgcg tttcaatttg cccgttactt
     7201 caacaaatca atcaaccgta acagagccac taatagctcc agtacagcaa tgagcacggt
     7261 taacttttgt gtcagcgtgt ccatccgctc tccttattaa ggagcacaac accagcacct
     7321 tacccttaca ctgcctgtcg atactggttt acgtttgttg agtcgccatg ttgcactccg
     7381 gatcagagca ctacctgcgg caccacccgt ccggtagcca ggacttgaaa taggttcgat
     7441 ccggcgcaac ttcccaacag tcaggattat aaatctcatt atcttaattg caacactgtc
     7501 cagaggtggt tataattcgt tcgttggaac acgatgttgc acaggcagtg gtgttaggcc
     7561 tgaaaatagt aaataaaaga acctgcctca ccagcagttt tttttattta ctgtgatctg
     7621 ctttccagat atttttcgct caaacaacta atgcgccaaa catttattgc gcgtaaaata
     7681 tcgtttattt cattaataca tttcagggat gaatatatgt caccgcagaa taatcatctg
     7741 cagcgtccgc ctgctgctgt gttatacgcc gatgaactgg caaaattaaa acaaaatgat
     7801 aacgcacctt gcccgcccgg ttggcagtta agtttgcctg cggcccgtgc ttttatcctt
     7861 ggcgatagcg cgcaaaatat tagccgtaaa gtggtgatta gtccctccgc tgtcgaacgt
     7921 atgttagtca ctcttgccac cggacgcggt ttgatgttgg tgggggaacc tggcaccgca
     7981 aaatctctcc tgtctgaatt actggcaacc tctattagcg gcgatgccgg gttaaccatc
     8041 cagggcggcg catctactac cgaagatcaa atcaagtacg gctggaacta cgccctgctt
     8101 atcaatcatg gtccatcaac ggaagcgctg gtccctgccc cgctttacca gggaatgcgc
     8161 gacggcaaaa tcgtccgttt tgaagagata acgcgtacac cgctggaagt gcaagactgt
     8221 ctgttaggta tgctttctga cagggtgatg acggtgccgg aactcactgg tgaagccagt
     8281 cagctctatg cgcgagaagg ctttaatatt attgccactg ccaatacccg cgatcgcggc
     8341 gtcaatgaaa tgagtgctgc gctaaagcgt cgcttcgatt ttgaaactgt ctttccgatt
     8401 atggatttcg cccaggagct ggagcttgtc gccagtgctt cggcgcgttt gctggcccat
     8461 agcggtattc cgcataaagt gcctgacgcg gtactggagt tactggtccg caccttccgc
     8521 gatctgcgtg ccaacggcga aaagaaaacg tcaatggata cactaacagc gattatgtcc
     8581 accgctgaag ccgtgaacgt ggcccacgcc gtaggcgtcc gcgcctggtt tttagcgaat
     8641 cgcgcaggtg agccagccga cctggtggac tgcattgccg gaaccatcgt caaagataac
     8701 gaagaagatc gcgctcgtct gcgccgttac ttcgaacagc gtgtcgccac acataaagaa
     8761 gctcactggc aggcttatta tcaagcccgc caccgcctgc cgtgaggaaa gatgcatgag
     8821 cgagccgtta attgtcggca tccggcatca tagtccggcc tgcgcccggc tggtgaaatc
     8881 gttaatcgaa agccagcggc cacgatacgt gttgattgaa ggccctgctg attttaatga
     8941 ccgggtagac gaactgtttt tagcccacca gcttccggta gctacttaca gttattgcca
     9001 gtatcaggac ggtgcagccc ccgggcgtgg tgcctggacg ccatttgctg aattttcgcc
     9061 ggagtggcag gcgctacaag ccgatcgtcg tattcaggca caaacttact tcatcgattt
     9121 gccttgctgg gcgcagagtg aagaagagga cgattcgcct gatacgcaag aggaaagcca
     9181 gaccttgctg ctgcgtgcca cccgcatgga taacagcgac accctgtggg atcacttgtt
     9241 cgaagatgaa agccagcaaa ctgcattacc ctctgcactg gcgcactatt ttgcccaact
     9301 gcggggcgac tcccccggcg atgctctaaa tcgtcagcgc gaagccttta tggcccgctg
     9361 gattggatgg gcgatgcagc aaaataatgg cgacgtgtta gttgtctgcg gtggctggca
     9421 cgctccggca ctggcaaaaa tgtggcgcga atgtccgcag gtaattaaca cgccagaatt
     9481 gccgtcgctg gcagatgccg ttacaggttg ttatctcacc ccctacagtg aaaagcgcct
     9541 tgatgtgctg gcaggatacc tttcaggaat gcctgccccg gtctggcaaa actggtgctg
     9601 gcagtggggc ttacagcagg cgggagaaca actgctaaaa acggttctca cccgtttgcg
     9661 ccagcacaag ttgcctgctt cgaccgcgga tatggctgcc gctcatctgc atgcgatggc
     9721 actggcacag ttgcgcggtc atacactacc gttacgcact gactggctgg atgccatagc
     9781 aggatcgctg attaaagaag ccctgaatgc gccgttgccg tggagctatc gcggcgttat
     9841 tcatcccgat accgatccga ttctgctaac gttgatagac acattagcgg gtgacggatt
     9901 cggtaaactt gccccttcta cgccacaacc gcctctgcca aaagatgtca cttgcgaact
     9961 ggaacgtacc gcaatctctc ttccggcgga gcttacctta aatcgcttta accccaatgg
    10021 actagcgcaa agtcaggtgt tacatcggct ggcaatactg gagatcccag ggattgtacg
    10081 ccagcaggga agcacactga cacttgcagg caacggtgaa gaacgctgga aattaacccg
    10141 cccgcttagc cagcatgcgg cattgattga ggccgcctgc tttggtgcca cactcctgga
    10201 agccgcacgc cataaattag aagccgatat gctggacgcg ggtggaatcg gcagcattac
    10261 cacatgtctt agccaagcgg cgtcagcggg tctggcgtcc ttcagtcaac aattactgga
    10321 gcaactcaca ttattaatcg cccaggaaaa tcaatttgcc gaaatgggcc aggcgctgga
    10381 agtgctatat gccttatggc ggctggatga aattagcggt atgcaaggcg cgcagatatt
    10441 acaaacgacg ttatgcgcgg ctatcgatcg cacgctgtgg ctgtgtgaat ccaacggcag
    10501 accggatgaa aaggagtttc acgctcactt gcatagctgg caagcgttgt gtcatatcct
    10561 gcgcgatcta catagcggtg tgcaactccc cggcgtttcg ctttctgcgg cggtagcctt
    10621 gctggagcga cacagtcagg cgattcatgt cctggcactg gatcgcggcg cgactcttgg
    10681 tgcgctgatg cgtctggaac atcccaacgc tagtgccgaa gcggcgctga cgatgctggc
    10741 gcagttatcc ccggcacaat ccggcgaggc gctgcacggt ttgctggcat tagcccgtca
    10801 tcaactggcc tgtcagccgg catttatcgc cggtttcagc agtcatttaa atcaactaag
    10861 tgatgccgat tttaccaatg ccctgcccga tttacgcgcg gcaatggcct ggctaccgcc
    10921 acgagaacgc gggacgctgg cgcatcaggt gcttgagcat tatcaactgg cgcaacttcc
    10981 cgtttcggca ctgcaaatgc cgttgcattg tccaccgcaa gccattgcac atcatcaaca
    11041 actcgaacag caggcactgg catcgctgca acactgggga gttttccatg tctgaactga
    11101 acgatcttct gaccacccgt gagctacaac gctggcgatt aattcttggc gaagcggcag
    11161 aaacgacgct ttgtgggctg gatgacaacg cccggcagat agatcacgcg ctggagtggc
    11221 tgtatgggcg cgatcctgaa cggctccagc gtggtgaacg ctctagtgga ttaggtggct
    11281 caaatctcac cactcctgag tggatcaaca gtattcacac gctgtttccg caacaggtga
    11341 ttgagcggct ggaaagcgat gccgtactgc gctacggcat tgaagatgtg gtgacaaatc
    11401 tcgacgtgct ggaacgtatg cagccttccg aaagcctgct acgcgccgtt ttgcacacca
    11461 aacatctgat gaaccccgaa gtactggctg ccgcccgcag gattgtgcgc caggttgtgg
    11521 aagaaattat ggcgcgactg gcaaaggaag ttcgtcaggc tttttctggt gtccgcgatc
    11581 gtcgtcgccg ctcatttatt ccactggcgc gaaactttga tttcaaaagt actctgcgcg
    11641 ccaacctgca acactggcac ccgcaacacg gcaagttgta tatcgaattc ccccgcttta
    11701 acagccgcat taaacgccaa agcgagcaat ggcaactggt cttactggtt gatcaaagcg
    11761 gatcgatggt cgattcggtg atccactctg cggtgatggc ggcctgttta tggcagttac
    11821 ccggcattcg tacccatctg gtggcgtttg acacaagcgt cgttgatctc acggcagacg
    11881 ttgccgatcc ggtagagtta ttaatgaaag tgcaattagg cggcggaacc aatatcgcca
    11941 gtgccatgga gtatggtcgg caacttattg aacaacctgc aaaaagcgtc attatcctcg
    12001 tgagcgattt ttatgaaggg ggttcatcat cattgctgac gcaccaagtg aaaaagtgtg
    12061 tccagagcgg catcaaagtg ctgggactgg cagcgctcga tagcacagca acaccttgct
    12121 atgaccgcga tacggcccag gcgctggtta atgtcggcgc acaaatagcc gccatgacac
    12181 cgggcgagct ggcatcatgg cttgcggaga atcttcagtc atgaattcac tacgtccgga
    12241 attattagaa ctgacaccgc aggcactgac ggcgttaagc aatgccggtt ttgttaagcg
    12301 cagccttaag gaactggaaa atggcaacgt cccggaaatc agccatgaga acagcgcttt
    12361 aatcgccacc ttcagtgacg gtgtccgtac ccagctggcg aacggccagg cactgaaaga
    12421 ggctcagtgc acctatgggg ccagcggcat gtgccgtcat cgcgtgatgc tggtgttaag
    12481 ttatcaacga ctttgtgcta ccgcccagcc tacgggaaaa gaagaggaat gggatccggc
    12541 aatctggctg gaagaactgg ctacccttcc cgatgccacc cgcaaacgcg cacaggcgct
    12601 ggtcgctaaa agcatcacca ttgagttgtt ctgtgcgccg ggtgaaattc cctctgcccg
    12661 cttaccgatg agcgatgtgc gtttttattc ccgcagcagt attcgtttcg cccgttgtga
    12721 ttgtattgaa ggcacacttt gcgaacatgt cgtactggca gtacaggcct ttgtccaggc
    12781 aaaagcgcag caagcggaat tgactcattt aatctggcag atgcgcagcg aacacgtcac
    12841 atcatctgac gatccgtttg ccagcgaaga aggcaaaacg tgtcgtcaat atgttcaaca
    12901 actaagccag gcattatggc tgagcggcat cagccagccg cttattcact acgaggccgc
    12961 tttcagccgc gcgcagcaag cagcggaacg ctgcagctgg cgatgggtga gtgaatcgct
    13021 acggcaactg cgcgccagcg ttgatgcctt ccacgcccgc gccagccact atcatgccgg
    13081 agaatgctta cgtcagcttg cggcattaaa cagtcgatta aattgcgcac aagagatggc
    13141 ccggagcgac agtgttggtg aagttcctcc tgtgccgtgg cgcacggtcg ttggctctgg
    13201 cattgccgga gaagcaaagc ttgatcatct gcggctggtg tctttaggta tgcgttgctg
    13261 gcaggatatt gagcattatg gtttacgcat ctggtttacc gatcccgaca ccggcagtat
    13321 tttgcacctt tcgcgcagtt ggccgcgaag tgaacaggaa gactcaccgg cagctacgcg
    13381 tcggctgttt agttttcagg ctggcgcact ggcgggtggg caaattgttt cacaagcagc
    13441 aaaacgcagt gccgatggcg agctgctgtt agctacccgc aaccgcttaa gcagcgttgt
    13501 accgctgtcg cctgatgcct ggcaaatgtt gagcgcgccg ttacgccagc cgggcattgt
    13561 ggctttgcgg gaatatttac gccagcgtcc ccccgcctgc atacggcctc ttaatcaggt
    13621 cgataactta tttattctgc cggtcgctga gtgtatttcg ctcggttggg atagcagccg
    13681 ccagacgctg gatgcgcagg taatcagcgg cgaaggtgaa gataatctgc tgacgttatc
    13741 actgccagcc tcagccagcg cgccttatgc cattgaacgc atggcggcgc ttttgcaaca
    13801 aacagacgac cccgtgtgtc tggtttctgg ctttgtcagt tttgttgatg ggcaattgac
    13861 actggaacca caggtaatga tgacaaagac tcgtgcctgg gcgctggacg cagaaactgc
    13921 gcctgtggtg gtatcactac cttctgccag cgttctgcct gtgccgtcta ctgctcatca
    13981 gttgctgatg cgctgccagg cgttacttat tcaactgctc cataacggct ggcgctatca
    14041 ggaacagagc gctattaatc aggcagagtt gctggcgaat gacctcaccg cggtgggttt
    14101 ttatcggctg gcacatgtgt tggcacaatt tcgtaataca gaaagcgagg cacgggtaga
    14161 agcaatgaat aacggtgttt tgctttgcga acaattattc cccatgcttc agcaacaagg
    14221 atgaaatagt gctttttact aagagttcta ctccagttcc ggactgctca cgccacggta
    14281 ttaggcatat cctatatagc ccctggtgag agtcacca
//
LOCUS       DAJTSA010000098        13964 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000098 DAJTSA010000000
VERSION     DAJTSA010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.098"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="OO369_003196"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="OO369_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5983364.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="OO369_003197"
     CDS             2219..2767
                     /locus_tag="OO369_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR5983365.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="OO369_003198"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="OO369_003198"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR5983366.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="OO369_003199"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="OO369_003199"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR5983367.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="OO369_003200"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="OO369_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR5983368.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OO369_003201"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OO369_003201"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR5983369.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="OO369_003202"
     CDS             complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="OO369_003202"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR5983370.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9765..12380
                     /gene="pepN"
                     /locus_tag="OO369_003203"
     CDS             9765..12380
                     /gene="pepN"
                     /locus_tag="OO369_003203"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR5983371.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OO369_003204"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OO369_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5983372.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..>13964)
                     /gene="ssuC"
                     /locus_tag="OO369_003205"
     CDS             complement(13351..>13964)
                     /gene="ssuC"
                     /locus_tag="OO369_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCR5983373.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3551 a         3563 c         3527 g         3323 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggtctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgctgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agttttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctatcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagagtg cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcgggggtg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcttcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tgggggggat acctatgtta cctggcatct gcaatcagac
     8221 aagcagaatt cgcaaatgca gcgaaagatt tcggaattaa aggtaaaaaa gggagccgat
     8281 tagctccctg atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca
     8341 gcgcccgaac aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg
     8401 ccaccggttt accgttacac tctaccaact taatcacaat attcaggggt tttacctggg
     8461 ggatatcgca ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt
     8521 ggcggtatag ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac
     8581 tctgtggatc aattcccagc ttttcataat gtgcaatggc tttttcaccc cattcaaccg
     8641 ggtcgccaga gtcatgacgc aggccctgat accgactagc gaactcgaca ccgaaatcac
     8701 gcaggaaagc atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatact
     8761 cttccagcca ggcagcaagt gcagctcgct ggctgttggc tagatccggg ctgatttgct
     8821 gatgtgcctg gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg
     8881 ccagatcgta gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg
     8941 tttcttgtac ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg
     9001 acatatcaag accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga
     9061 gggcttgcgc aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg
     9121 ccagcaaagg aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca
     9181 gcttgccatt atcgttggac acggtgactt gttccgggtt aaagcggaac tcgcgtaacc
     9241 agttaagata gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct
     9301 gcaggcgcag gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca
     9361 gcagatcgtc acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa
     9421 acacggcttg ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag
     9481 gagaagcgaa ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa
     9541 taaacatctt caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca
     9601 caccatatgc cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca
     9661 ataaaggcgt ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga
     9721 atagactgaa caccagactc tataaaagat gctaaaggtt atttatgact caacagccac
     9781 aagccaaata ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga
     9841 cctttgacct cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg
     9901 gtgcatcaga tgctccgctt cgtctcaacg gcgaagacct caaactggtt tctgttcata
     9961 ttaatgatga gccgtggacc gcctggaaag aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcgttttacg cttaagatca ttaatgaaat aagcctggcg gcgaatacgg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaatcaaa tatcccttcc tgctttccaa tggtaaccgc gttgcgcaag
    10261 gcgaactcga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgataccttt accacgcgtt
    10381 ctggtcgcga agtagcactg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactccatga aatgggatga agaacgcttt ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccggttgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggta aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagcga tcagcaactg accttcctga tgcgtcatgc gcgtaatggt ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atagacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcgt aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgccg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggtggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctt tacgagaaga
    12361 taactaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 taatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctgccgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctgaccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tatcatcctg aatttcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc ggccagcagg tgcagcaggg
    13201 tacttttacc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcatatcgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtct gtttgcaaaa actctcgcgc
    13501 attcatcgcc agataaccaa tgccggaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcatcggg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatctccac
    13681 cagcccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaacagcgt
    13741 accgagcgcc accagaaaga ttttcgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13801 cgggatcagc gccagatgcg gcacgttgcg caacatctga attgaggtat ccagcagcct
    13861 ttctccccag cgcgacagtc cgctaatcag tcccagaatc aggcccagcg atccgccaat
    13921 tgaaaagcca atcagcgccc gccaggaact gatcgccaga tgct
//
LOCUS       DAJTSA010000099        13249 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000099 DAJTSA010000000
VERSION     DAJTSA010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13249
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.099"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(16..999)
                     /gene="kdgT"
                     /locus_tag="OO369_003206"
     CDS             complement(16..999)
                     /gene="kdgT"
                     /locus_tag="OO369_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCR5983374.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="OO369_003207"
     CDS             complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="OO369_003207"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR5983375.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2164..3198
                     /gene="rhaT"
                     /locus_tag="OO369_003208"
                     /pseudo
     CDS             2164..3198
                     /gene="rhaT"
                     /locus_tag="OO369_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="OO369_003209"
     CDS             complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="OO369_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR5983376.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="OO369_003210"
     CDS             complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="OO369_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR5983377.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5241..6710
                     /gene="rhaB"
                     /locus_tag="OO369_003211"
     CDS             5241..6710
                     /gene="rhaB"
                     /locus_tag="OO369_003211"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR5983378.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6707..7966
                     /gene="rhaA"
                     /locus_tag="OO369_003212"
     CDS             6707..7966
                     /gene="rhaA"
                     /locus_tag="OO369_003212"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR5983379.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QHHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8208..9032
                     /gene="rhaD"
                     /locus_tag="OO369_003213"
                     /pseudo
     CDS             8208..9032
                     /gene="rhaD"
                     /locus_tag="OO369_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9042..9356
                     /gene="rhaM"
                     /locus_tag="OO369_003214"
     CDS             9042..9356
                     /gene="rhaM"
                     /locus_tag="OO369_003214"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR5983380.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9657..10103
                     /locus_tag="OO369_003215"
     CDS             9657..10103
                     /locus_tag="OO369_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR5983381.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            10114..11565
                     /locus_tag="OO369_003216"
     CDS             10114..11565
                     /locus_tag="OO369_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR5983382.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11555..12625
                     /locus_tag="OO369_003217"
     CDS             11555..12625
                     /locus_tag="OO369_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5983383.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12625..>13249
                     /locus_tag="OO369_003218"
     CDS             12625..>13249
                     /locus_tag="OO369_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR5983384.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT         3158 a         3459 c         3491 g         3141 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc ccagcttctt ctttcgtacc
      601 gtactgctgc atgatggaag cgtaaagtcc gccgttggtc atatccatcg ccgccaccag
      661 cgccagcgtt gaaagtccgg caaagaatcc aacttcaaca ccatgttccg gaataatgcg
      721 cgaggcaatt gccgcaacca cccacgcgac ggcaatttta gttaccacca gcgtaccgga
      781 tttacgcagt accgttcccg tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca
      841 caccgccaga atgggcaccg taccggtaat catcccgttg gtgaatgatc caaaatattt
      901 ccccgccccc ggcgagaagg tgtggcacag tgcgccaagg aatagcggga cgagcatcat
      961 cccccccggg attttctcaa tcgagcgttt tatctgcatt tccatcacct gtcggcatat
     1021 ttttaagggc tgatgcgtaa ttcctcaatg gagcatcagt gttgatggaa acgaagatag
     1081 acattcattt aatcaataaa agtgatcaaa atcacatcaa ttgaaacgct gttttatttt
     1141 tataggacca ttgtttttat tttggaattg atcacaaaaa aaccaccgcc gttggcgatg
     1201 gttcattttt taagctgata tgcggcctat acgcctcatt gcagcaggcg gcaaatgatt
     1261 attttttcgc cgcaaaacgt gccgctgctt cgtcccagtt caccacgttc cagaactctt
     1321 tgatgtagtc cggacggcgg ttctggaatt tcaggtagta agcatgttcc cacacatcca
     1381 ggcccagaat cgggaagccg gaagcgccag aaatagcttc acccatcagc ggagaatcct
     1441 ggttagcagt agaaaccacc gccagtttat cgcctttcag caccagccat gcccagccgg
     1501 aaccaaagcg ggaagctgcc gctttttcaa attctgcttt gaagttatca acggagccga
     1561 agtcacgttc gatagccgct ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt
     1621 tccagaacag gctgtggtta gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt
     1681 ctgctggcag ctggtccagt ttggtgatca gctcttcaac cggcaggttg gcaaattctg
     1741 gcaggctttc cagcgccgcg ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt
     1801 ggatttccat ggtctgctta tcgaagtgcg gttccagggc atcgtaagca tacggcaggg
     1861 atggcagggt atagctcata ttcatctcca gtattgtcgg gcggccgatt gttaatgccg
     1921 cgtaagcagt tggttcatta tagttaatta aatgatattg aaaatgatta tcaatgccgt
     1981 acttttcgta agggtatggt tttgcaggaa aatgcccgag atgtgaagca aatcacccac
     2041 ttaatgccgt gattgccagt aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca
     2101 gcacgatttt tacactcatc tcgtcggaga tgtgaagcga cgaaaaatga tgaggataag
     2161 aagatgagta acgcgattac gatggggata ttttggcatt tgatcggcgc ggccagtgca
     2221 gcctgttttt acgctccgtt caaaaaagta aaaaaatggt catgggaaac catgtggtca
     2281 gtcggtggga ttgtttcgtg gattattctg ccgtgggcca tcagcgccct gttactaccg
     2341 gatttctggg cgtattacag ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc
     2401 ggcgctatgt gggggatcgg taatatcaac tatggcctga ccatgcgtta tctcggcatg
     2461 tcgatgggaa ttggcatcgc cattggcatt acgttgattg tcggtacgct gatgacgcca
     2521 attatcaacg gcaatttcga tgtgctgatt aacaccgaag gcggacgcat gacgttactc
     2581 ggcgttctgg tggcgctgat tggcgtaggg attgtaactc gagccgggca gttgaaagag
     2641 cgcaagatgg gcattaaagc cgaagagttc aatctgaaaa aagggctggt gctggcggtg
     2701 atgtgcggca ttttctctgc cgggatgtcc tttgcgatga acgccgcaaa accgatgcat
     2761 gaagccgctg ccgcacttgg cgtcgatcca ctgtatgttg ctctgccaag ctatgttgtc
     2821 atcatgggcg gcggcgcgat cattaacctc ggtttctgtt ttattcgtct ggcaaaagtg
     2881 aaggatttgt cgctaaaagc cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg
     2941 ttactctcgg cactgggcgg gttgatgtgg tatctgcaat tctttttcta tgcctagggc
     3001 cacgcccgca ttccggcgca gtatgactac atcagttgga tgctgcatat gagtttctat
     3061 gtattgtgcg gcggtatcgt cgggctggtg ctgaaagagt ggaacaatac aggacgccgt
     3121 ccggtaacgg tgttgagcct cggttgtgtg gtgattattg tcgccgctaa catcgtcggc
     3181 atcggcatgg cgaattaatc tttctgcgaa ttgagatgac gccactggct gggcgtcatc
     3241 ccggtttccc gggtaaacac caccgaaaaa tagttactat cttcaaagcc acattcggtc
     3301 gaaatatcac tgattaacag gcggctatgc tggagaagat attgcgcatg acacactctg
     3361 acctgccgca gatattgatt gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa
     3421 acgcgctcac tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg acttttcagg
     3481 ctagccgcca gccgggtaat cagcttatcc agcaacgttt cgctggatgt tggcggcaac
     3541 gaatcactgg tgtaacgatg gcgattcagc aacatcacca actgcccgaa cagcaactca
     3601 gccatttcgt tagcaaacgg cacatgctga ctactttcat gctcaagctg accgataacc
     3661 tgccgcgcct gcgccatccc cacgctacct aagcgccagt gtggttgccc tgcgctggcg
     3721 ctaaatcccg gaatcgctcc ctgccagtca agattcagct tcagccgctc cgggcaataa
     3781 ataatattct gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc gtcagcatga
     3841 atataaaaga gatcgccacg ggtaatgcga taaggacgat cgttgagtac atgcagacca
     3901 ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg tatgttcggc aaagacatct
     3961 tgcggataac ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg
     4021 agaagtttta actgatgcgc caccgtggct acctcggcca gagaacgaag ttgattattc
     4081 gcaatatggc gtacaaatac gttgagaaga ttcgcgttat tgcagaaagc catcccgtcc
     4141 ctggcgaata tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt
     4201 actgtcgctg aatccacagc gataggcgat gtcagtgacg ctggcctcgc tgtggcgtag
     4261 tagatgtcgg gctttcatca gtcgcaggcg gttcaggtat cgctgaggcg tcagtcccgt
     4321 ttgctgctta agctgccgat gtagcgtacg cagtgaaaga gaaaattgat ccgccacggc
     4381 atcccaattc acctcatcgg caaaatggtc ctccagccag gccagaagca agttgagacg
     4441 tgatgcgctg ttttccaggt tctcctgcaa actgctttta cgcagcaaga gcagtaattg
     4501 cataaacaag atctcgcgac tggcggtcga gggtaaatca ttttcccctt cctgctgttc
     4561 catctgtgca accagctgtc gcacctgctg caatacgctg tggttaacgc gccagtgaga
     4621 cggatactgc ccatccagct cttgtggcag caactgattc agcccggcga gaaactgaaa
     4681 tcgatccggc gagcgataca gcacattggt cagacacaga ttatcggtat gttcatacag
     4741 atgccgatca tgatcgcgta cgaaacagac cgtgccaccg gtgatggtat agggctgccc
     4801 attaaacaca tgaatacccg tgccatgttc gacaatcaca atttcatgaa aatcatgatg
     4861 atgttcagga aaatccgcct gcgggagccg gggttctatc gccacggacg cgttacaaga
     4921 cggaaaaaaa tccacactat gtaatacggt catactggcc tcctgatgtc gtcaacacgg
     4981 cgaaatagta atcacgaggt caggttctta ccttaaattt tcgacggaaa accacgtaaa
     5041 aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag catcacatca
     5101 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
     5161 attttcctgt cagtaacgag aaggtcgcga attcaggcgc tttttagact ggtcgtaatg
     5221 aaattcagca ggatcacatt atgacctttc gcaattgtgt cgccgtcgat ctcggcgcat
     5281 ccagtgggcg cgtgatgctg gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg
     5341 aaatccatcg ttttaacaat gggctgcata gccaaaacgg ttatgtcacc tgggatgtgg
     5401 atagcctgga aagtgccatt cgccttggat taaacaaggt gtgcgaggaa gggattcgta
     5461 tcgatagcat tgggattgat acctggggcg tggactttgt gctgctcgac caacagggtc
     5521 agcgtgtggg cctgcccgtt gcttatcgcg atagccgcac caatggcctg atgccgcagg
     5581 cacaacagca actcggcaaa cgcgatattt atcagcgtag cggcatccag tttctaccct
     5641 tcaatacgct ttatcagttg cgtgcgctga cggagcaaca acctgaactt attccacaca
     5701 ttgctcacgc tctgctgatg ccggattact tcagttatcg cctgaccggc aagatgaact
     5761 gggaatatac caatgccacc accacacaac tggtcaatat caatagcgac gactgggacg
     5821 agtcgctact ggcgtggagc ggggccaaca aagcctggtt tggtcgcccg acgcatccgg
     5881 gtaatgtcat tgggcactgg atttgcccgc agggtaatga gattccggtg gtcgccgttg
     5941 ccagccatga taccgccagc gcggttatcg cctcgccgtt aaacggttca cgcgccgctt
     6001 atctctcttc tggcacctgg tcattgatgg gcttcgaaag ccagacgcca tttaccaatg
     6061 acacggcgct ggcagccaac atcaccaatg aaggcggggc ggaaggtcgc tatcgggtgc
     6121 tgaaaaatat tatgggctta tggctgcttc agcgagtgct tcaggaacgg caaatcaacg
     6181 atctcccggc gcttatcgcc gcgacacagg cacttccggc ctgccgcttc atcatcaatc
     6241 ccaatgacga tcgctttatt aatcctgacg agatgtgcag cgaaattcag gctgcgtgtc
     6301 gggaaacggc gcaaccgatc ccgggaagtg atgctgaact ggcgcgctgt attttcgaca
     6361 gtctggcgct gctgtatgcc gatgtgttgc atgagctggc gcagctgcgc ggtgaagatt
     6421 tctcgcaact gcatattgtc ggcggcggct gccagaacac gctgctcaac cagctatgcg
     6481 ccgatgcctg cggtattcgg gtgatcgccg ggcctgttga agcctcaacg ctcggcaata
     6541 tcggcatcga gttaatgacg ctggatgaac tcaacaatgt ggatgatttc cgtcaggtcg
     6601 tcagcaccac cgcgaatctg accaccttta cccctaatcc tgacagtgaa attgcccact
     6661 atgtggcgca gattcactct acacgacaga caaaggagct ttgcgcatga ccactcaact
     6721 ggaacaggcc tgggagctgg cgaaacagcg tttcgcggcg gtggggattg atgtcgagga
     6781 ggcgctgcgc caacttgatc gtttacccgt ttcaatgcac tgctggcagg gcgatgatgt
     6841 ttccggtttt gaaaacccgg aaggttcgct gaccgggggg attcaggcca caggcaatta
     6901 tccgggcaaa gcgcgtaatg ccagtgagct acgtgccgat ctggaacagg ctatgcggct
     6961 gattccgggg ccgaaacggc ttaatttaca tgccatctat ctggaatcag atacgccagt
     7021 ctcgcgcgac cagatcaaac cagagcactt caaaaactgg gttgaatggg cgaaagccaa
     7081 tcagctcggt ctggatttta acccctcctg cttttcgcat ccgctaagcg ccgatggctt
     7141 tacgctctcg catcccgacg acagcattcg ccagttctgg attgatcact gcaaagccag
     7201 ccgtcgcgtt tcggcctatt ttggcgagca actcggcaca ccatcggtga tgaacatctg
     7261 gatcccggat ggtatgaaag atatcaccgt tgaccgtctc gccccgcgtc agcgtctgct
     7321 ggcagcactg gatgaggtga tcagcgagaa gctaaaccct gcgcaccata tcgacgccgt
     7381 tgagagcaaa ttgtttggca ttggcgcaga gagctacacg gttggctcca atgagtttta
     7441 catggggtat gccaccagcc gccagactgc gctgtgcctg gacgccgggc acttccaccc
     7501 gactgaagtg atttccgaca agatttccgc cgccatgctg tatgtaccgc agttgctgct
     7561 gcacgtcagc cgtccggttc gctgggacag cgatcacgta gtgctgctgg atgatgaaac
     7621 ccaggcaatt gccagtgaga ttgtgcgtca cgatctgttt gaccgggtgc atatcggcct
     7681 tgacttcttc gatgcctcta tcaaccgcat tgccgcgtgg gtcattggta cacgcaatat
     7741 gaaaaaagcc ctgctgcgtg cgttgctgga acctaccgct gagctgcgca agctggaagc
     7801 ggcgggcgat tacactgcgc gtctggcact gctggaagag cagaaatcgt tgccgcggca
     7861 ggcggtctgg gaaatgtatt gccaacatca cgatacgcca gcaggtagcg aatggctgga
     7921 gagcgtgcgg gcttatgaga aagcgatttt gagccagcgt gggtaaacac tgccggatgc
     7981 ggcacgagcg ccttatccgg cctacgggtc gaatgtccat gcgggctgtc accctcaccc
     8041 cggccctctc cctgaaaggg cgagggagaa aaacatagcc tcgtgcggca acggagctga
     8101 acaccgctcc ggacagtccc ctcgccccta cggggagagg gttagggtga ggggaacaag
     8161 ccgcaccatg tctaaacaag gcaacctaat ttacaggaac acagaacatg caaaacatta
     8221 cccagtcctg gtttgtccag ggaatgatca aagccaccac cgatgcctag ctgaaaggct
     8281 gggacgagcg caacggcggc aacctgacgc tgcgcctgga tgacgccgat atcgcgcttt
     8341 atcacgacaa cttccaccca caaccgcgct atattccgct cagccaaccc atgcctttac
     8401 tggcaaatac gccgtttatt gtcaccggct ccggcaaatt cttccgtaac gtccagctcg
     8461 atcctgcagc taacttaggc gtcgtaaaag tcgacagcga tggcgcgggc taccatattc
     8521 tctgggggtt aaccaacgaa gccgttccaa cttctgaact tccggctcac tttctctccc
     8581 actgcgagcg cattaaagcc accaacggta aagatcgggt gatcatgcac tgccacgcca
     8641 ccaacctgat cgccctcacc tatgtactgg aaaacgacac cgcggtcttt actcgccaac
     8701 tgtgggaagg cagcaccgaa tgtctggtag tattcccgga tggcgttggc attctgccgt
     8761 ggatggtgcc cggcacggac gaaatcggcc aggcgaccgc acaagagatg caaaaacatt
     8821 cgctggtgtt gtggcccttc cacggtgtgt tcggttccgg accgacgctg gatgaagcct
     8881 ttggtttaat cgacaccgca gaaaaatcag cacaagtatt agtgaaggtt tattcgatgg
     8941 gcggcatgaa acagaccatc agccgtgaag agttgatagc gctcggcaag cgcttcggcg
     9001 ttacaccact agccagtgcg ctggcgctgt aaggagcaaa catgatccgc aaagcctttg
     9061 tcatgcaggt aaaccccgac gcccacgaag agtatcagcg tcggcataat cccatctggc
     9121 cggaactgga agcagtgttg aaatctcacg gtgtgcataa ctacgccatt tacctcgaca
     9181 aagcgcgtaa tctgctgttt gccatggtag agattgaatc tgaagaacgg tggaatgcgg
     9241 tagccagtac tgatgtttgc caacgttggt ggaaatatat gaccgatgtt atgcccgcta
     9301 acccagataa cagcccggtg agtagcgagc tgcaagaagt gttttacctg ccgtaattgc
     9361 ccaatgtgcc ggagatgcct ccggcacaat attttaaatt tcgatttgaa aaattaaaca
     9421 tattacatct cccggtaagt tatatttccc tgatacattg tgagtaaatc acaaaaataa
     9481 tgaataaccc attaatgatt catgtggttt atttaaataa ccattatgtg cattactccg
     9541 caaatctgac ctttcactct gtctcaaatt gtcattgttt accgacatat cgcctctcat
     9601 cacttaatcg ctccaccatt acaattgatt aaaatatatt ttatggagtc cgattaatgg
     9661 cagctcttac tgcaagctgt attgacctga atattcaggg caatggcgct tattccgttc
     9721 tgaagcagtt ggcgacaata gcgttacaaa acggttttat caccgactca caccagttcc
     9781 tgcaaaccct gctgctgcgc gaaaaaatgc actctactgg atttggttcc ggtgtcgcag
     9841 tgccgcacgg taaaagcgcc tgcgttaaac aaccgttcgt attattcgcc cgcaaagcgc
     9901 aggctattga ctggaaagcc agcgatggcg aagacgtcaa ttgctggatc tgcctcggcg
     9961 tgccgcaaag cggcgaagag gatcaggtca aaatcatcgg cacactgtgt cgcaaaatta
    10021 ttcaccagga atttattcat caactgcaac agggcgatac cgaccaggtg ctggccttgt
    10081 taaatcaaac cctcagctca taaggaagtg gcgatggagt catccttacg tattgtcgcg
    10141 atcaccaact gccccgccgg gatcgctcac acctacatgg tggcggaagc cctggaacag
    10201 aaagcccgtt ctctcggcca taccataaaa gtggaaactc aagggtccag tggcgttgaa
    10261 aaccgcttat ccagcgaaga gattgccgct gccgattacg tcattcttgc taccgggcgt
    10321 ggcctgagcg gtgatgatcg cgcgcggttt gccgggaaga aagtttatga gattgccatc
    10381 tccctggcgt tgaaaaatat cgaccagatt ttcagcgaat taccgacaaa ctcgcagctt
    10441 tttgccgcag atagcggcgt gaagcttggc aaacaggaag tgcaaagcgg cagcgtgatg
    10501 agccacctga tggcgggcgt ctctgcggcg ctgccgtttg tcatcggcgg tggtatcctg
    10561 gtggcgcttg ccaacatgct ggtgcagttc ggtttgccgt ataccgatat gtcgaaaggc
    10621 gcgccgtcgt ttacctgggt agttgaatcc atcggttacc ttggcttcac ctttatgatc
    10681 cccatcatgg gcgcttatat tgcctcgtcg attgccgata aaccggcttt cgctccggcg
    10741 tttctggttt gctatctggc gaacgacaaa gcgctgctcg gcacccagtc gggtgcggga
    10801 ttcctcggcg cggtggtgct ggggctggcg attggctatt tcgtcttctg gttccgcaaa
    10861 gtgcgccttg gcaaagcatt acaaccgctg ctcggttcaa tgctgatccc gttcgttact
    10921 ctgctggttt tcggcgtgct cacttactac gttatcgggc cggtgatgtc cgacctcatg
    10981 ggcgggctgc tccacttcct gaataccatt cctccatcaa tgaagtttgc ggcagcgttt
    11041 ctggtcggtg cgatgctggc gttcgatatg ggcggcccca tcaataaaac cgcctggttc
    11101 ttctgcttct cactgctgga aaaacacatt tatgactggt acgccatcgt cggcgttgtc
    11161 gcgttgatgc cgcccgttgc ggcgggtctt gcgacgttca ttgcgcccaa actgtttacc
    11221 cgacaggaaa aagaggctgc cagtagtgcc attgtggtcg gtgccacagt cgcaaccgaa
    11281 ccggctatac cttacgctct ggcagctccg ttgccgatga ttaccgccaa tacgctggcg
    11341 ggcggcatta ccggcgtgct ggtgatcgct ttcggaatca aacgtctggc acctggtttg
    11401 ggtatctttg acccgctgat cggcctgatg tcgccggtag gttcgtttta tctggtgctg
    11461 gcgattggcc tcgcgctgaa catttcattc attatcgtcc tgaaaggatt gtggttacgc
    11521 cgcaaagcga aagccgcgca acaggagctt gtccatgaac attgagttac tgcaacagtt
    11581 gtgcgaagcc agcgccgtta gcggcgatga acaggaagtt cgcgacatcc tgataaacac
    11641 gctggaacct tgcgttaatg aaatcacctt tgatggtctg ggcagctttg ttgcccgtaa
    11701 gggaaataaa ggtccaaaag ttgccgttgt cgggcatatg gatgaagtcg gctttatggt
    11761 cacccacatc gacgagagcg gttttctgcg tttcaccacc attggtggct ggtggaatca
    11821 gtcgatgctc aaccatcggg taaccatacg cacacacaag ggagtgaaaa tccctggtgt
    11881 gattggtttc gtcgcgcctc atgcgttaac ggaaaagcaa aggcaacagc cgctgtcatt
    11941 tgatgagatg ttcattgata ttggcgcaaa cagtcgcgat gaggtggaaa aacgcggtgt
    12001 ggcgatgggc gattttatta gcccggaagc caattttgcc tgctggggcg aagataaagt
    12061 ggtcggcaag gcgctggata accgcatcgg ctgcgcaatg atggctgaac tattgcagac
    12121 ggtgaataat cccgaaatta cgctgtatgg cgtcggcagc gtggaagaag aagttgggct
    12181 acgcggggca caaacctcgg cggaacacat taaaccggat gtagtgatcg tgctggatac
    12241 cgctgtcgca ggcgatgttc cgggcattga taacattaaa tacccgctga aactgggcca
    12301 ggggccgggg ctgatgctgt ttgacaagcg ctacttcccc aaccagaaac tggtagcagc
    12361 gttaaaaaac tgtgccgcac ataacgattt accgctgcaa ttttccacca tgaaaaccgg
    12421 agcgacggat ggcgggcgct acaacgtgat gggcggcggg cgtccggttg ttgcgctgtg
    12481 cctgccaacc cgctatctgc acgctaacag cggcatgatt tcaaaagccg attacgatgc
    12541 tctgctcacg ttgatacggg gttttctgac gaccttaacc gcggagaaag tcaacgcgtt
    12601 tagccagttc cgtcaggtgg attaatgcta aacgaacgcc agttaaagat tgtcgatctg
    12661 ctggagcaac agccgcgcac gcctggtgag ctggcgcaac agactggcgt ttcaggcagg
    12721 accatcctgc gtgatattga ctatctcaac tttaccctta acggcaaagc ccgcattttc
    12781 gccagtggca gtgcgggcta tcagctggaa atcttcgagc gccgcagctt ttttcagttg
    12841 ctgcaaaagc acgataacga cgatcggctg ctggcgctgt tattactgaa tactttcact
    12901 ccccgtgcgc aactcgcctc ggcgcttaat ttgccagaaa cgtgggtagc agagcgtctg
    12961 ccccagttaa aacagcgtta tgaacgcact tgttgcctgg ccagccgccc tggtttgggc
    13021 catttcattg atgagacaga agagaaactc gttatcttgc tggcgaactt gctgcgcaaa
    13081 gatccgtttt taattccgct ggcgggcata acacgagaca accttcagca tttatccacg
    13141 gcctgcgaca accaacaccg ctggccgctc atacagggtg attatctctc cagcctgatt
    13201 ctggcgattt acgccctgcg taatcaactg accgatgagt ggccgcaat
//
LOCUS       DAJTSA010000100        13098 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000100 DAJTSA010000000
VERSION     DAJTSA010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13098
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.100"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..890)
                     /locus_tag="OO369_003219"
     CDS             complement(<1..890)
                     /locus_tag="OO369_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5983385.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="OO369_003220"
     CDS             1131..1931
                     /locus_tag="OO369_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5983386.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="OO369_003221"
     CDS             2009..2779
                     /locus_tag="OO369_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5983387.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="OO369_003222"
     CDS             complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="OO369_003222"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR5983388.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="OO369_003223"
     CDS             complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="OO369_003223"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR5983389.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5034..5756
                     /locus_tag="OO369_003224"
     CDS             5034..5756
                     /locus_tag="OO369_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5983390.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="OO369_003225"
     CDS             complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="OO369_003225"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR5983391.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6285..7016
                     /gene="dnaQ"
                     /locus_tag="OO369_003226"
     CDS             6285..7016
                     /gene="dnaQ"
                     /locus_tag="OO369_003226"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR5983392.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7149..7225
                     /locus_tag="OO369_003227"
     tRNA            7149..7225
                     /locus_tag="OO369_003227"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7183..7185,aa:Asp,seq:gtc)
     gene            complement(7356..8402)
                     /locus_tag="OO369_003228"
     CDS             complement(7356..8402)
                     /locus_tag="OO369_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCR5983393.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="OO369_003229"
                     /pseudo
     CDS             complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="OO369_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="OO369_003230"
     CDS             complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="OO369_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR5983394.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="OO369_003231"
     CDS             complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="OO369_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR5983395.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11362..12192)
                     /locus_tag="OO369_003232"
                     /pseudo
     CDS             complement(11362..12192)
                     /locus_tag="OO369_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="OO369_003233"
                     /pseudo
     CDS             complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="OO369_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3007 a         3393 c         3484 g         3214 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcac caatcgtcaa actttggcct ggcttcagct tagatccgcg
     3061 cagtttgttc cactgctgca aatctttggt gcttacgccg agacgtgaag cgatgcttga
     3121 aagcgtgtcg ccagagcgta cggtgtaaac acggctgtta agcggcgtat tgttggcaac
     3181 cagcgtcgac tgtacagcag caatttcgcc tgaagcgaga gattcacgca gttgatctgc
     3241 atgcttcttt ggcaccatca cgtactgcgg cccacttgcg cccagcgtgg agcctttcac
     3301 gccagcgttg aatgtcttca gcttgctgac ggaaatcccc gccatatctg caaccttcgc
     3361 catttcaacc gggctactca ggtgcacacg cgccagagca cggctttcat cggtcgttgg
     3421 cagacgtacg ccataacgct tgctgttttt gagaatatca ctcaatgcca gcattttagg
     3481 cacgtactgc ttcgtttcct gcggcaacgg taacgaccag aagtccgtgg atttcccacg
     3541 cgctttgttc gttttaattg ccttcatgac ccgaccttcg ccgctgttat aagccgctac
     3601 ggtcagaagc cagtcgtcgt caaacatttt gttcagacgc tgcatcatat tcagcgcggc
     3661 agttgttgaa gcaacaacat cgcgacgcgc gtcataattg cgggtctgtt tcaaaccata
     3721 attgcgcccc gtgctcggaa tgatctgcca gatgcctgcg gcattggcgc cagacgttgc
     3781 gtgaggatca aaagcgctct ccactatggg tagtagtacc agttccatag gcatgttacg
     3841 ttttttaact tgccctgcta tccagtacat atacggctct gcccgtaaag ttacatcgtg
     3901 gagatagctc ttattgcgta aatatttctg tttctgttcg cgaatccggt cattttccgg
     3961 aattcccatc tttagctcgt cgccaatgaa agcccacaag tcaccatctg gcgcgataga
     4021 cgtcccatcg tccatccatc gtgcctgact tgtaaacttt gctgcttccc cttgaccagc
     4081 tgcagaaagg ctctgtgcgt gctgttgaac gttgccggta ctctggcaac ccacgagcag
     4141 gacagaggcg agtaatatcg cttttgcctt catgtgtgtg tcaatagttg cttaaaagac
     4201 gaccgatcat aacggcgaac ggagccgatg acaagaaagt tttatcagaa cctatctttc
     4261 tttgacctta accatgcaaa acgctcttca ggttgttgca ataatgtttc ttcattaatt
     4321 ccattaatta aatcaatatc ttccgttctt aaaaaaacat taatttgccg ctcatttttc
     4381 agaattacgg gtagtgttat ttgatttttt gcccgtaact ccttaacttt acgataataa
     4441 tcatttatgg acaaatcgtg cggaaaaata ctcaaagcaa acttcatatt tgataacgta
     4501 tattcatgag cacaacatac caatgtatcg tcaggtaacg cacttagctt tttaagtgat
     4561 tgatacattt gtaaagctgt cccttcaaac aaccgaccac acccaccaga aaacagcgtg
     4621 tcgccgcaaa atagataagg tttactgaag taacagatat gtcctaaagt gtgacccggt
     4681 gtggtaatta cactaaattc atgccctaaa acgaaggcag tttcgccatc tttgactacc
     4741 tgtgttgttc ccttatcttg tgtctcttgt ggaccataca ctacaatttg cggaaacttt
     4801 tccaccagtt cttttacgcc gccaacgtga tcgtggtgat ggtgggttag aaatatggcc
     4861 tccggttgcc agttattggc ggtaatggcg tttaatactg gctctgcgtc tccgggatcg
     4921 acaatcaggc agcgacctgc ctcatcattc aaaacccaga tgtaattgtc atcaaaggcg
     4981 ggaatactgt taagattcat agattacctc tcagtgtaaa acggaaggtt gtgatgaaac
     5041 cggcaagagt ccctcaaact gtcgtggctc ctgattgctg gggcgatttg ccctggggag
     5101 agctttatcg caaggcgctg gagcgccagc tcaacccgtg gttcactaaa atgtatggtt
     5161 ttcatctgct taagattggc aatttaagcg cagaaatcaa ttgcgaagcg tgcgcggttt
     5221 ctcatcaagt gaatgtttct gcgcaaggaa tgcccgtcca ggtgcaggcg gatccacttc
     5281 atcttccttt tgccgataaa tccgttgata tttgtctact ggcacatacc ttgccgtggt
     5341 gcaccgatcc gcatcgttta ttacgtgaag ccgatcgggt actgattgat gatggctggc
     5401 tggtgattag tggcttcaac cccatcagtt taatgggatt acgcaaactt gtgccggtat
     5461 tgcgcaaaac ctcgccctat aatagccgga tgtttactct gatgcggcaa ctggactggc
     5521 tctctttgtt gaattttgaa gtgctgcacg ccagccgttt ccacgttctc ccgtggaaca
     5581 aacacggagg aaaactattg aatgcgcata ttcctgcgct tggttgctta caacttattg
     5641 ttgcccggaa acggactatt cctttaacgc taaatccgat gaaacagagt aaaaacaagc
     5701 cacgaattcg ccaggcggtt ggagccaccc ggcaatgtcg taaaccacag gcttaaactt
     5761 cgacttggta gcccgtatct tccagtgtgg gattcatcgc cgcggcacga gccagttcat
     5821 cacagcgttc gttttccggg tgtccggcat ggcctttaac ccattcccat ttgatttgat
     5881 gctgccccaa tgcagcatca agacgttgcc agagatcgac attttttact ggttttttgt
     5941 ctgctgtttt ccagccacgt tttttccagt tatggatcca ctgggtgata ccctggcgga
     6001 catactggct gtcggtactc aaaatgactt cgcaatgttc ttttaacgcc tccagcgcga
     6061 caatagcggc catcaactcc atacggttgt tggtggtgcg ggtgtagcct gcgctaaagg
     6121 ttttctcgcg tccgcgatag cgtaaaatag cgccgtaacc cccaggtcca ggattaccca
     6181 gacacgaacc atcggtgaaa atttctacct gtttaagcat ctctggtaga cttcctgtaa
     6241 ttgaatcgaa ctgtaaaacg acaagtctga cataaatgac cgctatgagc actgcaatta
     6301 cacgccagat cgttctcgat accgaaacca ccggtatgaa ccagattggt gcacactatg
     6361 aaggccacaa gatcattgag attggtgctg ttgaagtagt gaaccgtcgc ctgacgggca
     6421 ataacttcca tgtttatctc aaacccgatc ggctggtgga tccggaagcc tttggcgtac
     6481 atggtattgc tgatgaattt ttgctcgata agcccacgtt tgccgaagta gccgatgagt
     6541 ttatggacta tatccgcggc gcggagttgg tgatccataa cgcagcgttc gatataggct
     6601 ttatggacta cgagttttcg ttgcttaagc gcgatattcc gaagaccaat accttctgta
     6661 aggtcaccga tagccttgcg gtggcgagga aaatgtttcc cggtaaacgc aacagcctcg
     6721 atgcgctatg tgcccgctac gaaatagata acagtaaacg aacgctgcac ggggcattac
     6781 tcgatgccca gatccttgcg gaagtttatc tggcgatgac cggtggtcaa acgtcgatgg
     6841 cttttgcgat ggaaggagag acacagcagc aacaaggtga agcaacaatt cagcgcattg
     6901 tacgtcaggc aagtaagtta cgcgttgttt ttgcgacaga tgaagagctg gcagcacatg
     6961 aagcccgtct cgatctggtg cagaaaaaag gcggaagctg cctctggcgg gcataaatac
     7021 atgtaaaagg cgctaaaaat agcgacttgg gcgatttttg cagcaaacga ttcaaaagat
     7081 gagaaaaatc gttgacgaag gtcgaggcaa tccgtaatat tcgcctcgtt cccaacggaa
     7141 cacaacgcgg agcggtagtt cagtcggtta gaatacctgc ctgtcacgca gggggtcgcg
     7201 ggttcgagtc ccgtccgttc cgccaatatt cagaaagccc gaatcagtaa tgattcgggc
     7261 tttcgtcgtt ttggtgaagt gagaattttt cactccgaag taaaacggat atgatttaaa
     7321 aaatggccag acattataaa ttctggccac ttctgttact ccagcgcatc ccatttctcc
     7381 agcagttcta ctggaatttc atttattacc tccgagacgc gttttccgac caggcgttct
     7441 gcctggcgca gcaaaggaat ggtcgggctg ctcggttcgc tgtgctcaaa ccagcgacgg
     7501 atgacacgca ggcgttccag cgcgtcgttt cggtcgcgga tctgccgggg ttctgcgccg
     7561 gtcatcggtg atatttcctc cgcgatatgc accggtagcg caatctgctc tgcctgcatc
     7621 tgttcagtat gaacgatggt ggcgttttcc ggctgctcag cctgtggttg tggcaggatc
     7681 tccggcgtgg tgctctgcac tgctcccggt aacagggcca gcagttgcgt cagacgggag
     7741 aagtccggtg cctggtcatt cagcgtttca cgcgcatggc gttgcagccg ctcagtggtc
     7801 tctgctgcct ggcgaaaggc atccagcggc agtgtgccgc gcgcttccag gtctgccagt
     7861 tgctggcgta ccgattctgg tgccagcgca tccgccgggc gtaatgcaga tagcgaacgc
     7921 tccacatccc gcacctgaag gcgcatggca gcattatttg acagtgtaat gccacgaata
     7981 tccgccatta caccttcgtg gtccagcaac gccgccagcg cgttactgcg tgccaccgca
     8041 gcatcttcgg cggtgatatc ttcagtcgcg agcagttgag gatggatggc gtccgggtac
     8101 gtggagcaca acgtttccag cagcgtgagc gcctccgaca gtcccctggc accggcctgc
     8161 tggatacggc agcgcagcag gacaaccaga atgcggatat ctttgctgcg catcagcaga
     8221 cgatgggcgt cccgctcaag ctccgcccag ttaatgtttt ccggcgtact gacaaaatcg
     8281 ccatattgcg cttcttcacg cggtgcggcg cgggtgaaca gcagcaggta ttccggatcg
     8341 tactccagat ccgggccgca ggggcattcc gccgaaattg ggtcagacag gaatatttcc
     8401 atttaattat cctcagtgcg ccagcgtggt ggccagaccg tccggattgc tggactcacg
     8461 caccagatgg gccgatgccg ccagacgagc gtgatctggc aacgggctgt cagcgctctc
     8521 ccgcaaatcc tgcccgctga ggctggcgac ataaaaggca aagcggtcat gttgcggttt
     8581 atccagaccg cccccagcgg actgggcgct gcgccatgcc tggtaaaact gcgtcagcca
     8641 gcggtgatga aaaagatcag aaaaacgggt cagcgtcggc tttcagtccg gttacgcgcc
     8701 agttcggtgt agtgcagcgg cagtggaccg ttcggccccc acagcacgag gctgtacaga
     8761 ctgatgtgca ggcgcccttc ctgccagccc acactggcga tttctcgtgg cgcaaaggtc
     8821 agcgcgggcg tttgtccgag gcgaaatttt tccatccgtg gttgccaggt gttgcccgcc
     8881 ggaacggtac acaactgcac atccacgcgg cgcatcaggt tcagaaaacc gtaacgccag
     8941 gggggggcct gcgcctgagt gaaatcgttg ctcatacgct tccccgctgt cccgccctga
     9001 caggccaggt cataattttg ccgcgctgca tgctgtgcag cgtcatctgc gagaaggtat
     9061 taatggaaac atggcgggcg atatagtgtt ccagcaccag gccgaacaga tacggactga
     9121 tgccggaaaa accttcttca tccaccgtca gttcacaact gacgccacgc ccatagacca
     9181 gtagtccgga accgggcaga cggcgggtga ccggggtcgt tttacaacca atcaggctgc
     9241 gcacctggcg cgattgcgga ctgtcatgag caggaataaa caggttcagc agatctcgca
     9301 acgcctggcc gccggtacgg tgatccagat cggccagcgg cagataatta aaagataact
     9361 ggcggatcaa ccgccatgcc atctcacgtt cagccatcgg gggctgcggc gagcgtggcg
     9421 ggcggataag ccctacgccc gccaccggga tggctgcatc cacggttaga tcgtcccggc
     9481 cattgcgggc aataagacag ggcagatcgc ggttagtgac catggccgtg atggtgatgt
     9541 ggcgcagatt ttccgggtac ggcgcttcat actggtcaac cagcgacagg aacacttccg
     9601 agcccgtata gggggtacgg gtgccgtaac ggcgggcgtt ttctgataag cggcggggct
     9661 cccggcgcag ggaaaaataa cgtccgtgat tgccttcatc attattgcgg gtgtggtaga
     9721 gcgggcgaaa ggccatcttc cgtgtggtgt cggtttccag cccttccact tcctggaccg
     9781 agaacacctc ataatccagc ggatgggttc tgtccaccac cagatgctgt tccgtcgtgc
     9841 tgtgcgtgac atcgatccgg gcggtgacgc ggggaaacag gttgataacc ggggtacaga
     9901 acaggctgaa ctgtgccgca tttgtctggt ggatcagcca gtccggcggc aggcggttca
     9961 gcaggataac aatctccgcc acgccaccgt caattttctg taatccggca gacaggcctg
    10021 tcggggtgaa aaaatagaag cgttccggac aggcaaaata ttcatgcagc aggttatgac
    10081 cgtgaaacac gttccacgcc agcggcagca gcccctgatc cggctccagg ccttcgtaca
    10141 taacgggctg ttgcagattc acatccagcg cgccgtcaaa atgccccgga atgccagcca
    10201 ggggcgcgat ggcgctggta tgtagaagtt ctaacaggtg tgaggccgta cgctcctcgc
    10261 cgcagagata aaacggcagc ctgtccagtc cggcaagctg actgaacgtt agttcaccaa
    10321 aggtgcgcag ggtaatacgc agcgccccgg cgacgtgaat gttggccggc aggtagcggt
    10381 gcagggcagg catatccggt ggcgcggcgg tcagtcgcgc ctcttcgatg gcgagcggcc
    10441 acagcgttac atcctggctg ctgcgaaact ggcaggcagt gctttcgcct tccggaatgg
    10501 cggagaaaaa ggctgtgtcg cgcgggacgg taaatccttt cgccagatcg ccttcttccg
    10561 tatcgggatg caactgcgcc acgctcattg agggcgtggg tgtgacgtaa ttggggctga
    10621 ccacttccag caggcgctgg gtgaaacggg ggaattcagc gtcaattttt agctgcgtgc
    10681 gggcggtcag gaagctgaag gcctcgacca ttcgctctac atacggatcg gcaatatccg
    10741 ttccctgcat gcccagccgg gcggcgactt tgggatgacg ggcggcaaat tcggctcccg
    10801 tttcccgcag atagctcagt tcgcggttgt agtattccag tagccgtgga tccatcggtc
    10861 tctctctgaa ttaaaaaaac gtaatgcgac tctgctccat atccagcgcg ctacgtaccc
    10921 ggaatgcagt cggatacggc tgcgtgagga tctcgccgcg aatttcaaac tgtaatgtgt
    10981 tatggccact ctggcggcgc tcatcctgaa gtgggatgat ttgcagagtg gacgctttca
    11041 gccggggttc aaaccgggtg atggttcgca aaatggccct gcggatatcg tcccacttgt
    11101 gctcatacag aaagctgccc gccagtggcg gcaggccgta gttaagtacg gacgctgccg
    11161 cgtacggata acgtgcagca tcaatatcat cttcatggct gacggtgttg agcagaaatg
    11221 acaaatcccg gcggatgatc tccttaatct gaaccggact gacgctgata ccgtggtcgc
    11281 gtttctggtt tggggcgtcg tcacacaggc ggtcaaacag cgtgggcagc aggtgattgc
    11341 ctggacgctg gcgggaagcg ctcacgcgtc gcttccttca gcggtgttaa aggtacaggt
    11401 ggacaaatcc agcaggctga tatcgccgtg accggtgagc cacaccctct gacccagcgc
    11461 ctgtaccagt gtctctcccg gaccgtcctg ccagaccgtt tcccggcaca gacgcaggga
    11521 gtcggcagcc ttttcagagc cgctgtagcg gggaaacagc taggcactgt gtgtatcgcc
    11581 attgttcagg gtgacgttga cgggtttcca gatcaaatcg atcagatgcg caggccgcgg
    11641 cgatttcagc gaacagattt gtgaaaacgg cagccagata tatgctccgc ccgcgatcag
    11701 ttcgagtacc ggaccggtac gggagtcgct gtcgctaatc cagtcaaatg cgccgccatt
    11761 ccactgcccg gaagtgtctg ttatggcctc aagcgcgcgg ctgcggtgag catcagcttc
    11821 ctgcgcttca ccacgggcgt tacaggcgag ggcggtcagc agatcctcca tccaggcggg
    11881 cggcgggaga ataacaccgg gccgctgttc gccctgaaag caggcgtggc ggtatatctc
    11941 acagcggatc agctcgccaa atacctgcgc ttcgcgagta taatttgcgt ccatccgggc
    12001 gcatagctgg atctgttgca gtgcccgcga ccagtcgcct gccacgcaca ataactgaaa
    12061 caggctgtgg cggcgggagg cctgcgctgg gtgcatcctg acctgttgct ctgccatctg
    12121 aatgccctcc accacggaat aatcccgcag cagcgccgac aaggtaatcg gtagctcgtt
    12181 attgtttgtc atgatatatc tctccatgtt gtgccgggtt tatccggtaa tggctgtcaa
    12241 tgccaatgat gcgatgctct ttccgtgtca gatctggcag tacggtgtta tgcgctgttt
    12301 ccggggcgta ttccggggaa agtaaatgca ggatatcagg gggagtcttt tcgacaggcc
    12361 agatagtttc atcttcgcca gtggcatcca gtacattgag aatagcgtca atgcttaacc
    12421 caccagtgac catatcctgc aatgtctcgg cttcggtgac caggtcacgc aatgaatcca
    12481 gatcctgcga ggagggctct tcgttaaaag gaaaaggatc agcatggcga ttaagatctt
    12541 gttcgggaga taacaacgcc tcgcggtatt cctgataaag tcgggtcagg gcatttcctt
    12601 tttccgcacc ttctccggtg gaggaggctc catgaacagg aatgatatcg aatggatttt
    12661 gtgatgtcag atgctgctgc ctgaaccaat ccagatccag atattcggtt attgcttccg
    12721 acgtgatggc agtctgcgat atcgttttta ctgtgctcag gttcggagat gatggattgc
    12781 catgtacaag gcactatgaa gataaccccc attctatcag gtcgccttca ttcagacgca
    12841 tacggtgatg aggttccaca atctggtcgt ttaccgtaca gcaatattcg gtagactgat
    12901 tgataatcca ccatgcttct tcgtggcggg tcagagtgag ggcaatcttt acatcatcat
    12961 tcaccttaaa aacaggaatc cagggagagg taacggtgaa gataatttgt gttccttcag
    13021 tcgcgtaaat tgtgttatcg gcgatcccgt gcgcctgtat tttttggagt ccccacgtaa
    13081 tatttggttt cattgtgt
//
LOCUS       DAJTSA010000101        12907 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000101 DAJTSA010000000
VERSION     DAJTSA010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12907
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.101"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OO369_003234"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OO369_003234"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR5983396.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="OO369_003235"
     CDS             complement(1806..3224)
                     /locus_tag="OO369_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR5983397.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3378..3722)
                     /locus_tag="OO369_003236"
     CDS             complement(3378..3722)
                     /locus_tag="OO369_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR5983398.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="OO369_003237"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="OO369_003237"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR5983399.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5647)
                     /locus_tag="OO369_003238"
     CDS             complement(4712..5647)
                     /locus_tag="OO369_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR5983400.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="OO369_003239"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="OO369_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR5983401.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKVPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="OO369_003240"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="OO369_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR5983402.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="OO369_003241"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="OO369_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR5983403.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9084..9518
                     /gene="marR"
                     /locus_tag="OO369_003242"
     CDS             9084..9518
                     /gene="marR"
                     /locus_tag="OO369_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR5983404.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9538..9921
                     /gene="marA"
                     /locus_tag="OO369_003243"
     CDS             9538..9921
                     /gene="marA"
                     /locus_tag="OO369_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR5983405.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9953..10171
                     /gene="marB"
                     /locus_tag="OO369_003244"
     CDS             9953..10171
                     /gene="marB"
                     /locus_tag="OO369_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR5983406.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="OO369_003245"
     CDS             complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="OO369_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR5983407.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11365..12483
                     /gene="ydeE"
                     /locus_tag="OO369_003246"
     CDS             11365..12483
                     /gene="ydeE"
                     /locus_tag="OO369_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR5983408.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12610..12705
                     /gene="mgtS"
                     /locus_tag="OO369_003247"
     CDS             12610..12705
                     /gene="mgtS"
                     /locus_tag="OO369_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCR5983409.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3398 a         3299 c         2951 g         3259 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aaggaatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc cgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataaacgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatctgc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtggtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagttcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcattcat ttgacttata cttgcctggg caatattacc ccctacaact
     9061 aattacttgc caaggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     9121 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     9181 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     9241 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     9301 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     9361 agcgcggcgt actggtaaaa cttaccacca gcggcgcggc aatatgcgaa caatgccatc
     9421 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     9481 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     9541 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     9601 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac
     9661 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
     9721 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
     9781 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
     9841 gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
     9901 ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc
     9961 actttcatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagagcg ttgcggaaca
    10021 aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga
    10081 acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg
    10141 cgtgccctat tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt
    10201 attaacttcc cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
    10261 cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata
    10321 gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca acccgccagg
    10381 tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg
    10441 ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg
    10501 atgcggaacc atcgagaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc
    10561 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
    10621 agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg
    10681 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
    10741 cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga
    10801 tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt
    10861 taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga
    10921 gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca
    10981 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
    11041 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
    11101 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
    11161 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
    11221 acttcggatt tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct
    11281 ggtagagcgt cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt
    11341 attttagaga tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt
    11401 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
    11461 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
    11521 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
    11581 gtgacgctgg ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctacc
    11641 gtgctgaaag cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca
    11701 atcaactaca ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg
    11761 gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg
    11821 cttttcattc aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
    11881 gtctggtcgc cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt
    11941 tttctggctt cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg
    12001 attgctgatg gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc
    12061 atggtggtta cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg
    12121 ctgatgacag caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc
    12181 aacaacctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat
    12241 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
    12301 ttttccgccc agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta
    12361 gtgctaacca gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct
    12421 gcgtgggtgc tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt
    12481 tgatttaagt cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa
    12541 tatctttctc attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg
    12601 aggcaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt
    12661 tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc
    12721 cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg
    12781 aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt
    12841 tacgctaaca ggcattttcc tgcactgata acggatcgtt gacacagtag catcagtttt
    12901 ctcaatg
//
LOCUS       DAJTSA010000102        12766 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000102 DAJTSA010000000
VERSION     DAJTSA010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12766
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.102"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..283
                     /gene="ydiV"
                     /locus_tag="OO369_003248"
     CDS             <1..283
                     /gene="ydiV"
                     /locus_tag="OO369_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR5983410.1"
                     /translation="GAGNSTMKAVFDGLFTRVMLEKGFIQQQITHRSFEPFIRAIQAQ
                     ISPCCNCIIAGGIDTPEILAQITPFDFHALQGCLWPAVPINQITTLVQR"
     gene            347..1783
                     /locus_tag="OO369_003249"
     CDS             347..1783
                     /locus_tag="OO369_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR5983411.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(1787..1978)
                     /gene="hemP"
                     /locus_tag="OO369_003250"
     CDS             complement(1787..1978)
                     /gene="hemP"
                     /locus_tag="OO369_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR5983412.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2110..3156)
                     /gene="aroH"
                     /locus_tag="OO369_003251"
     CDS             complement(2110..3156)
                     /gene="aroH"
                     /locus_tag="OO369_003251"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR5983413.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(3313..4146)
                     /gene="ppsR"
                     /locus_tag="OO369_003252"
     CDS             complement(3313..4146)
                     /gene="ppsR"
                     /locus_tag="OO369_003252"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR5983414.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            4478..6856
                     /gene="ppsA"
                     /locus_tag="OO369_003253"
     CDS             4478..6856
                     /gene="ppsA"
                     /locus_tag="OO369_003253"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR5983415.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(6913..8559)
                     /gene="fadK"
                     /locus_tag="OO369_003254"
     CDS             complement(6913..8559)
                     /gene="fadK"
                     /locus_tag="OO369_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR5983416.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(8616..8909)
                     /locus_tag="OO369_003255"
     CDS             complement(8616..8909)
                     /locus_tag="OO369_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5983417.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(8906..10195)
                     /locus_tag="OO369_003256"
     CDS             complement(8906..10195)
                     /locus_tag="OO369_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5983418.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(10251..11189)
                     /locus_tag="OO369_003257"
     CDS             complement(10251..11189)
                     /locus_tag="OO369_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR5983419.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(11209..11973)
                     /locus_tag="OO369_003258"
     CDS             complement(11209..11973)
                     /locus_tag="OO369_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR5983420.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            12294..>12766
                     /locus_tag="OO369_003259"
     CDS             12294..>12766
                     /locus_tag="OO369_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR5983421.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDALAYTLA"
BASE COUNT         3065 a         3209 c         3335 g         3157 t
ORIGIN      
        1 gggagcaggt aatagcacaa tgaaagcggt ttttgatggt cttttcaccc gcgtaatgct
       61 ggagaaaggt tttattcagc aacaaatcac gcatcgctcc ttcgaaccgt ttattcgtgc
      121 gatccaggcg caaatttccc cttgttgcaa ttgcatcatc gcggggggca ttgatacgcc
      181 agaaatattg gcacagatta cccccttcga tttccatgct ctccagggat gcctgtggcc
      241 tgccgttcct ataaatcaga taacgacgtt ggttcagcga taaccctcct gtttgctggt
      301 gtttaagacg agagtaaccg tctacactat caaacagata ggatctatga ccctgtcttt
      361 tattacccgc tggcgcgatg aattgccaga aacctataca gcactttccc ctacgccttt
      421 aaataatgcc cggctgattt ggcataatac cgaactcgct aacacgctga gtattccatc
      481 gtcgctgttt aaaaatgccg caggtgtctg gggcggcgaa accctactgc ctggtatgtc
      541 accactggca caggtttaca gtggtcatca gttcgtcgtc tgggcgggcc aactgggtga
      601 tgggcgcggc attttacttg gcgaacaact gcttgctgat ggcactacaa tggactggca
      661 tctgaaaggt gctggcctga cgccttattc gcgaatgggt gatggacggg cggttttacg
      721 ttcgacgata cgagaaagtc tcgccagtga ggcgatgcat tatctgggca ttccgacgac
      781 ccgcgcgtta agtatcgtca ccagcgattc gccagtgtat cgggaaacgg tggagccagg
      841 cgcgatgctg atgcgtgtgg caccaagtca tctgcgcttt ggtcatttcg aacattttta
      901 ctatcgccgc gagccggaaa aggttcgtca gttggctgac tttgccattc gtcattactg
      961 gtcacatctt gaagacgatg aggacaaata ccgtctctgg tttaacgatg ttgtcgcacg
     1021 taccgcgtcg ttaattgccc aatggcagac ggtcggcttt gcacatgggg tgatgaatac
     1081 cgacaacatg tcgctgctgg gactgacgct tgattacggg ccgtttggtt ttcttgatga
     1141 ttacgaaccc ggttttattt gtaatcactc ggatcatcaa gggcgttata gctttgataa
     1201 tcaacctgct gtcgcgttgt ggaatttaca gcgtctggcg cagacattgt caccatttgt
     1261 tgccgtagat gccctgaatg aggccctgga cagctatcag caggttttgt tgacgcatta
     1321 tggacaacgg atgcggcaga aactgggctt catgacggag caaaaagagg ataacgcgct
     1381 actgaatgaa ttattcagtc tgatggcgcg agagcgcagc gattataccc gcacattccg
     1441 catgctgagt ctgaccgagc agcacagcgc ggcgtcaccg ctacgtgagg agtttattga
     1501 tcgtgcggca tttgatgact ggtttgcccg ttatcggggg cgtttgcaac aagacgaggt
     1561 tagcgatagt gagcgtcagc aactgatgca aagcgttaac ccagctctgg tattgcgcaa
     1621 ctggttggcg caacgggcga ttgaagcggc agaaaagggt gatatgatgg aattgcaccg
     1681 cctgcatgag gcgttgcgaa atcctttcag cgacagagat gatgactatg tcagtcgtcc
     1741 acctgactgg ggtaaacggc tggaagtcag ctgctcgagt taaaggctac ttagttaaca
     1801 gcagcttgcc agcctgagtt ttacggagca gatattcttg cccgtcatga tcgataatca
     1861 gtttgccatc cggacctaac agtgtctggc tggtaattcg ccggatgggc tgcggggaag
     1921 cggtcttgtg attggcatcc gtttcaggcg tgagttttct gctatccgta taacgcataa
     1981 aaattaataa taattgatat aacaatgatt cctattatca atattatggc agaccagagc
     2041 aagcagtaat gtgttgagaa gacaacaagt tgcgcagaaa tgcccatccc atcaggaatg
     2101 ggcacgcatt cagaagcggg tatctaccgc aaaggcgagt ttttcgacca ggcgttcggt
     2161 atcttcccag cccagacacg ggtcggtaat ggattgaccg taagtgagcg gctgaccgcc
     2221 gacgattttt tgcgttcctt cgcgcaggaa actttccgcc ataattccag caatctccgt
     2281 agagccattg cggatttgct gacaaatatc ctcacaaact tctaactggc gacggtgctg
     2341 cttctggcag ttaccgtggc tgaaatccac caccagatgt tcaggtaaat caaactcgtg
     2401 cagcgtatcg caggctgcgg cgatatcatc ggcatgataa ttcggttttt tgccgccacg
     2461 cataataatg tggccatacg ggttgccgct ggtctgatag atggtcatct gaccattttt
     2521 gtctggcgag aggaacatat ggctggcgcg ggctgcgcgg atagcatcca cggcaatccg
     2581 cgtgttgcca tcggtaccat ttttaaaacc taccggacag gagagtgctg aagccatttc
     2641 gcggtggatc tgactttcgg tagtacgagc gccaatcgcg ccccaactga ttaaatcagc
     2701 aataaactga ccggttacca tatcgaggaa ctcagtcgcg gtagggacac ccagctcatt
     2761 tacctgtaaa agtaatttgc gcgccagctc cagaccgtga tttacccgat agctgccgtt
     2821 taaatccgga tcggagatta gccctttcca gccgacaaca gttcgtggtt tttcaaaata
     2881 ggtgcgcatt acgatttcca gccgtgactg gtactggttg cgcagcgact gcagacgggt
     2941 ggcgtactcc attgcagcgg tgagatcgtg gatcgagcag gggccaataa tgaccaacag
     3001 tcgcttatct tcaccattca gtattttttc aattctgcgg cgggagtcgg tgacatgggt
     3061 ggcgacgcca ggcgttacgg gataccgtag cgcgagttcg gcgggcgtta ccagactctc
     3121 aatacgcgca gtacggagtt cgtcagttct gttcattaca agtctcagaa atttgttgct
     3181 tctacggcag gactaccgga aagtgatgcg catcacgata aaccaatcgc agctgatttc
     3241 aagtgcgagg tgtgtcacac ctcgccgggt ggttagcatg ataacaaaaa aacaaaccaa
     3301 tgcactagtt ctctagtaca ttcggcgact aaggcccatg atatcgagga tcttggtggc
     3361 aatctcttct accgaataat tggtactgtt aatccacggg atctgatttt tacggtacaa
     3421 ggcttccact tccgcgactt ccatcctgca ctgacgaagc gaggcatagc gactgttctc
     3481 ccgacgttcc tcgcgaatcg ccgccagacg ttccgggtcg atagtcaggc cgaacaattt
     3541 atgctgaagc ggtttgagcg acgcgggtag caccagatta tccatatcgt cggcaataaa
     3601 ggggtagttt gccgcgcgga taccaaattg cattgccaga tacagactgg tgggggtttt
     3661 accacagcga gaaacaccga gcaggatcac ctgagcctga tccagattgc gcaacgaaat
     3721 gccgtcatcg tgggcgaggg tgtaatcaat cgccgcaatg cgcgcatcat atttattgag
     3781 attattaggg ttaaggccat gggtacgatg agcaatcggc gttggatcca gtttcatctc
     3841 ttgttgtagc ggggcaacca gcgcctgaac gatatcctgg caaaagcctt cactttgcaa
     3901 gatgatggcg cgaatctccg gcaacacgat ggagtagaag accagcgggc gcacgcctgt
     3961 ctggtgataa attgcgtcaa tctgatcctt cactgcccgt gcacggctct cattttcgac
     4021 aaacggcagc gtgatgctgc tgatagtgac gggaaattgt gacattactg cgtgtcctaa
     4081 tacctccgca gttattgccg taccatcaga aatataaaaa acgtggcgat caacagcatt
     4141 atccattttg ttcttcccgt gaagcagaca taattctatg aaagcataaa ttaaaagcgc
     4201 acagaagcgt aggacgtaat gtctggttta taaaatgaac cttcatttta ttttttataa
     4261 aaacagcatt tcatttttat ggtttcgttt ataccgatgg tttatgtgga aattgtcgaa
     4321 tagagcaggt ttgcgcaacg ctgggatcag tcttaaaaag taaaaaaata tatttgcttg
     4381 aacgattcac cgtttttttc atccggttaa atatgcaaag ataaatgcgc agaaatgtgt
     4441 ttctcaaacc gttcatttat cacaaaagga ttgttcgatg tccaacaatg gctcgtcacc
     4501 gctggtgctt tggtataacc aactcggcat gaatgatgta gacagggttg ggggcaaaaa
     4561 tgcctccctg ggtgaaatga ttactaatct ttccggaatg ggtgtttccg ttccgaatgg
     4621 tttcgccaca accgccgacg cgtttaacca gtttctggac caaagcggcg taaaccagcg
     4681 catttatgaa ctgctggata aaacggatat tgacgatgtt actcagcttg cgaaagcggg
     4741 cgcgcaaatc cgccagtgga ttatcgacac tcccttccag cctgagctgg aaaacgccat
     4801 acgcgaagcc tatgcacagc tttccgccga tgacgaaaac gcctcttttg cggtacgctc
     4861 ctccgccacc gcagaagaca tgccggacgc ttcctttgcc ggtcagcagg aaaccttcct
     4921 caacgttcag ggttttgacg ccgttctcgt ggcagtgaaa catgtatttg cttctctgtt
     4981 taacgatcgc gccatctctt atcgtgtgca ccagggttac gatcaccgtg gcgtggcgct
     5041 ctccgccggt gttcagcgga tggtgcgctc cgacctcgca tcttctggcg tgatgttctc
     5101 cattgatacc gaatccggct ttgaccaggt ggtgtttatc acttccgcat ggggccttgg
     5161 ggaaatggtc gtgcagggtg cggttaaccc ggatgagttt tacgtgcata aaccgacact
     5221 ggcggcgaat cgcccggcta tcgtgcgccg caccatgggg tcgaaaaaaa tccgcatggt
     5281 ttacgcgccg acccaggagc acggcaagca ggttaaaatc gaagacgtac cgcaggaaca
     5341 gcgtgacatc ttctcgctga ccaacgaaga agtgcaggaa ctggcaaaac aggccgtaca
     5401 aattgagaaa cactacggtc gcccaatgga tattgagtgg gcgaaagatg gccacaccgg
     5461 caaactgttc attgtgcagg cgcgtccgga aaccgtgcgc tcacgcggtc aggtcatgga
     5521 gcgttatacg ctgcattcac agggtaagat tatcgccgaa ggccgtgcta tcggtcatcg
     5581 catcggcgcg ggtccggtga aagtcatcca tgacatcagc gaaatgaacc gcatcgaacc
     5641 tggcgacgtg ctggttactg acatgaccga cccggactgg gaaccgatca tgaagaaagc
     5701 atctgccatc gtcaccaacc gtggcggtcg tacctgtcac gcggcgatca tcgctcgtga
     5761 actgggcatt ccggcggtag tgggctgtgg agatgcaaca gaacggatga aagacggtga
     5821 gaacgtcact gtttcttgtg ccgaaggtga taccggttac gtctatgcgg agttgctgga
     5881 atttagcgtg aaaagctcca gcgtagaaac gatgccggat ctgccgttga aagtgatgat
     5941 gaacgtcggt aacccggacc gtgctttcga cttcgcctgc ctgccgagcg aaggcgtggg
     6001 ccttgcgcgt ctggaattta tcatcaaccg tatgattggc gtccacccac gcgcactgct
     6061 tgagtttgac gatcaggaac cgcagttgca aaacgaaatc cgcgagatga tgaaaggttt
     6121 tgattctccg cgtgaatttt acgttggtcg tctgactgaa gggatcgcga cgctgggtgc
     6181 cgcgttttat ccgaagcgcg tcattgtccg tctctctgat tttaaatcga acgaatatgc
     6241 caacctggtc ggtggtgagc gttacgagcc agatgaagag aacccgatgc tcggcttccg
     6301 tggtgcgggc cgctatgttt ccgacagctt ccgcgactgc ttcgcactgg agtgtgaagc
     6361 agtgaaacgt gtgcgcaacg acatggggct gaccaacgtt gagatcatga tcccgttcgt
     6421 gcgtaccgta gatcaggcga aagcggtggt tgaagaactg gcgcgtcagg ggctgaaacg
     6481 tggcgagaac gggctgaaaa tcatcatgat gtgtgaaatc ccgtccaacg ccttgctggc
     6541 cgagcagttc ctcgaatatt tcgacggctt ctcaattggc tcaaacgata tgacgcagct
     6601 ggcgctcggt ctggaccgtg actccggcgt ggtgtctgaa ttgttcgatg agcgcaacga
     6661 tgcggtgaaa gcactgctgt cgatggctat ccgtgccgcg aagaaacagg gcaaatatgt
     6721 cgggatttgc ggtcagggtc cgtccgacca cgaagacttt gccgcatggt tgatggaaga
     6781 ggggatcgat agcctgtctc tgaacccgga caccgtggtg caaacctggt taagcctggc
     6841 tgaactgaag aaataaaata aatccccggc ggcgttcagt cgccggggtt atgtgatccc
     6901 cgaagatgaa acttattcaa tctcttcaca gacatcctgc gttaatcgcc gtataatatc
     6961 ttttcttaac aaaaactttt gtattttatc tgaggcagtg cgcggtagtt tttcgattac
     7021 cacgatatgt tcaggatatt tatattttgc gacccgttta cggctaaaaa aagccgccat
     7081 ctcttccagc gataatgaat gatgcggcgc tttcagcacg acataagcgc atgatcgttc
     7141 acctaaacgt tcatcgggca ttgcaaccac acaggcatcg tgaattttag gatgctgcaa
     7201 taaaatatct tccacttcac ggctgctaat attttcgccg ccgcggacaa taatatcttt
     7261 tttgcgtccg gtaattttta tatagccagc ctcatccata cggcagagat cgccgctgta
     7321 ataccagcct tcttcatcca gcgcacgggc ggttaattca ggttcatcaa aataccccat
     7381 aaacacattg gggccacgcg atgcttcttc accttcgcaa cctggcggta aggtcttgcg
     7441 tgcgtcatcg accactttaa tctctacacc tgcggcagcg taaccatcgg tatgcataaa
     7501 gcgcgacaaa gaattatcga gattcaccac cgcatgcggc gaactttctg tggaaccata
     7561 aacacttaat aatttaatgc tgcgctgctg gcattcgcgc gccacttttt tggggattgt
     7621 ggtaccgccg caaagaaaga agcgcagcgc tgaaaggtct gcgggttgtt tctctagtaa
     7681 attcaaaaga tcatagacaa acggcgttgc gcctagcata caggtgcaac gctgctgctc
     7741 aagcagcgcg agacacgcat caggagtgaa aatatctaac aacacgctgc gagcgccaat
     7801 taagaatggt gccgttacgc catgcagaaa gcccgttgcg tgaccaagtg gcgcaggcat
     7861 cataaagaca tccagccagg tcagattcag ccgcgcgcaa taagcccgct cactggcgag
     7921 aatattgtta tgcgttagca tcacgccctt tggcagaccc tcggttccgg aggtaaacag
     7981 caccgcagct aattcatcgc cgtgggtcgt tatcgccgtg gtcagtggga tattgtcggc
     8041 gataatctga cttaatgaga gggaagaggt ggcgggagcc agtttgtcca cgccgacaat
     8101 ttgttgtagt tgtggaagct gattttgcag cggcaggatt aaatctaccg gacgcgtttg
     8161 tttaaacaac gtcggtgcaa agaacatttt tgcctgacac ttattgagca cccacaccag
     8221 ttctgcttcc cgccaggaag gcaacagcgg cacggaaacc gcaccgattt tcaggcaggc
     8281 aagatagata acggtaaatt cacaccagcc aggcagttga aatgcgatgc gatcgcctga
     8341 ttcaataccc ttcgctaaca tccagtttgc cagacagctc gcggcgtgat cgagcgcgct
     8401 ataggtgtac gatgcaccat gattatcgac cacggcaatt ttgtctggca tcgcacgagc
     8461 ggtctgctgc cagtaatcgg ccagtgaagc atcgccccat agcccttgct gacgatacgc
     8521 cgcacgacgt tgttcgttaa acgttaatgt cactttcatt ttggactctc gaaacagaac
     8581 atcaggcccg agttgcgggc ctgtgggatg catgattagc cgtagcgaaa atcaataccg
     8641 aaggtgcctg cgggatattg ccactgttcg agaatagtgt taccgcacag cacccgacag
     8701 gtgccgcact ccagacaacc ggcgggatca aaatgaatgt ttcctacgtc atcctgctta
     8761 taaagtccgg cagggcaggc tttcattaat ttacggaatt cattgatatc gggattttcc
     8821 gtcagaatga tatgcgggtg gccctcatca acatggaatt tattgacgcc taatttgatg
     8881 tcgacgttaa ccgtagcgtt ctggctcata gcgcggttgc tcccttaatg ccatctttca
     8941 gcaagttgat cagcccaatt ttcttcgcgt gtcccatgat cattttgcgt accggctggt
     9001 ttggtttgcc gtcaatggtg aacatctcgt tcatgatgtc ggctaccatt cgtgggtatt
     9061 ggctaaacag gcgcgggttt tccatcagcg ccgggatctt gcgaaaatgc tgcatatcac
     9121 gcatgacgca gctttgttcc agctcacgtt tgtattgcgc cagactgttg gcggagaaat
     9181 ccgcgcgttc tttggcggcg atcaccgttg tggcggcagc ctgagccgat gcaatggcta
     9241 aatccatgcc gcggaccgta aaacccaaat tcaggcagaa gcctgcggcg tcaccaacga
     9301 tcatcacgcc ctcgttaacc agttgcggca ccattgccag accgccttcc ggcaccatat
     9361 gcgcggaata ttcaagcagt ttgccgccgc taatcagcgg gcgaatggcg gggtgttgtt
     9421 taaaatcttc cagcatttgc ggcacgcttt tttgcgcatg ggcgatatca cccaatccac
     9481 aaaccagccc caaggatatg gaatccttgt tggtatagag aaatcccccg cccatcaggc
     9541 cgtcagaagg ggaaccggca aacagccagg cggcaccttc ctcgcccgta atattaaagc
     9601 gatcgttgat ctgttctggt gtgaggccaa taacctcttt aacaccaaca gcgtaatgat
     9661 gcggatcgga agcgggaacc attcccagcg agcggccaag catcgagtta acgccatcag
     9721 ccagaatcac cacattcgct tcgagaatat catccccagc ctgcacgcca gtgaccttgt
     9781 ttccttcacg aaccagcgcg tcgacgcgaa cgcctgggat aaactgcgcg cccgcctgct
     9841 cggcttgttc catcaaccac gggtccagac gattacgcaa tacggtataa gatgcgtgtt
     9901 gaggaacatc tggttgctcg cggtgaaaat cgagggtaac ggcgctttct tcggttaaga
     9961 aggagatttt ctcgcgtgtg accttgcgtt ctaccggcgc tgatactgca aagcctggaa
    10021 tgattgcttc aagtgtgtgg gcataaagac gcccgccggt catgttttta catccggcac
    10081 tgtcgccgcg ttctatcacc aggacatcca gcccggctcg tgccatgacc agtgcggcaa
    10141 cgctaccagc aacgcccgca ccgaccacaa tggcatcaaa tttgtcatcc gacatgcgtt
    10201 atgctccctt cctgctgcgt ttacagcggg agatgtggtt gaaaggggaa ttagcggctc
    10261 aactggctaa tcagggcagg gacgactttg tagatatcgc ccaccagacc gtagtcggca
    10321 tagttgaaga ttggcgcatt tttatcttta ttgatggcga caatcacttt tacgccgttg
    10381 ccgccaacca tatgttggat ctgcccggag atccccagcg tcaggtagag atcggatttc
    10441 agcaacacgc cggagacacc gatataacgt tcacgctcca tccagttctc gccttcggca
    10501 attggacgtg aacagccgac ttcagcattc agcaccgccg ccagttcgtg gaccattttc
    10561 aggtcatcct gcgccgccag accacgaccg acgccaacca cacgttttgc tttgctcagg
    10621 tccacgctgc ttgcggcttt cgcacggcgt tcgcgacaga gaatttcatg acgcggagca
    10681 acataaggta ccgtttctgt cgggcactga tgagaggtat cactggtgca cggttcctga
    10741 acaccgggtg caagggtaat gatcgccagc gggctgttga tcttttcctg agcgaacgct
    10801 aacccgccat acatccggtg ttcggcgcaa atgtgaccat cgacaatatc caccgccgtg
    10861 gcatcgttca ccagcgccgc attcagttgc acacttaacc gtgctgccag agctttacca
    10921 cgtttcgtcg cggccaacag caacatagcg gggtgtttat ctttcagcag ggcagcaatg
    10981 ctttcggcgt aattttcagt gcgttgcagc gcgtcgtttt gctcaagaac ataaatacat
    11041 tttggaccat aaggcataac tgcctgcgcc tggtcggtat tttgtacaat ggcatacacc
    11101 tgttggcccc attgctgagc gccgccaaac agttcagcat aacgttcagg attatcgcta
    11161 aagacccaga cgctgtttaa ttgactcatc gtttcttctc cgtaggcttc agttcagggc
    11221 tttcttcaga tgttccgcca gctcggcaat ggcctccggc gaatcgttat cgagaatgat
    11281 gtgcttacgt tctgtttgcg gcggtacgcg aatgccgacc agttcagcaa gtggcgcgct
    11341 ctggctccag tcaatatcac ttgcctgcca ctgatttacc ggttttttac ccgcgccgag
    11401 aatggctttc atcgaaggaa tgcgtggcac gttaatatcg gaggtgacgc agagcacggc
    11461 tggaacagag agttcaataa cttcaacatc atcttcaagc gtgcgttcaa tcaccagtgt
    11521 attgccctga cgctgaatag cactcactgc attaatcacc ggaagttgca gaatttctcc
    11581 gaccagcaag ccaacctgct gggcataaag gtcgccggaa ccttcaccaa agatcagtaa
    11641 atcgaagccg atcttttcaa ttgctgccgc cagcgctttt gcggtatcga gaggcagtgc
    11701 atgttcaagt tgcgcatcct gcaccaaata caggctgtgc ggcccgcggg atagcacgtc
    11761 tttgcgcact ttcgagttct gcaacaatga gccgccaacg gtcagcgcgg ctatctcatc
    11821 gtcatctgtt gcgagctggc ttgcagcttc aatggcattg agatcgaact ggctgatttt
    11881 ggcgtcggca ttgtcgaaat tcagggtgta ttctggagtg acaacaatgt cctgttcttc
    11941 aggcaccagc ttaaagcagg ttattatttt catgatttct cctgtgagtc gcatacggaa
    12001 ccatctccga tggaaggtaa aaatggcctg aaaatagtgt gcgatagcgg tgcggggggg
    12061 ggggggcgac ggagcaagct gttttccttg ccaacaatct tgcaaaaaaa atgcgattgc
    12121 taaaacaaaa gtgcaatttg cggcaatata gttattccat gaccgtacaa tcttgcattc
    12181 agtgtaaata atcttgttta gctctcgcta aacgcggttg tataaccgca atacacgccg
    12241 tattttatcc ggcatatatt gtttttgctt gctcatggaa tagggaggtt gtcatgtatc
    12301 aacgctgttt tgataatgcc agtgaaacgc tgtttgtcgc cggtaaaacg ccacggcttt
    12361 cacgttttgc atttagcgat gatccaaaat gggagtctgg acatcacgtt catgacaatg
    12421 aaaccgagct gatttacgtc aagaaagggg ttgcaaggtt taccatcgat tcttcgttat
    12481 atgtcgcaca tgcggatgac attgtggtga tagaacgcgg caggctgcat gcggtggcct
    12541 ctgacgttaa cgatccggca acgacgtgta cctgtgcgct gtacggcttt cagtttcagg
    12601 gggctgagga aaatcagtta ctgcaaccgc attcttgtcc ggtaattgcc gcggggcagg
    12661 gaaaagaagt cataaaaacc ttatttaatg agctaagtgt gattttgccg caaagtaaaa
    12721 atagccaaac atcttcgtta tgggatgcat tagcctatac attagc
//
LOCUS       DAJTSA010000103        12696 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000103 DAJTSA010000000
VERSION     DAJTSA010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.103"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="OO369_003260"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="OO369_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR5983422.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="OO369_003261"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="OO369_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR5983423.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="OO369_003262"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="OO369_003262"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR5983424.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="OO369_003263"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="OO369_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR5983425.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="OO369_003264"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="OO369_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR5983426.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="OO369_003265"
     CDS             4025..4615
                     /locus_tag="OO369_003265"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR5983427.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="OO369_003266"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="OO369_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR5983428.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="OO369_003267"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="OO369_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR5983429.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="OO369_003268"
     CDS             complement(6551..6658)
                     /locus_tag="OO369_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983430.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="OO369_003269"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="OO369_003269"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5983431.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="OO369_003270"
     CDS             7648..7875
                     /locus_tag="OO369_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR5983432.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="OO369_003271"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="OO369_003271"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR5983433.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..10703)
                     /locus_tag="OO369_003272"
     CDS             complement(9447..10703)
                     /locus_tag="OO369_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCR5983434.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSECCLD
                     NENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10964..11884
                     /gene="cbpA"
                     /locus_tag="OO369_003273"
     CDS             10964..11884
                     /gene="cbpA"
                     /locus_tag="OO369_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR5983435.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11884..12189
                     /gene="cbpM"
                     /locus_tag="OO369_003274"
     CDS             11884..12189
                     /gene="cbpM"
                     /locus_tag="OO369_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR5983436.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12282..>12696)
                     /gene="torD"
                     /locus_tag="OO369_003275"
     CDS             complement(12282..>12696)
                     /gene="torD"
                     /locus_tag="OO369_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5983437.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         3031 a         3242 c         3200 g         3223 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataatatt cactttttaa aaactggcat ccgctttgcg aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgcgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccacag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcatttta cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttagcg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct catctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcttatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 ggcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacgcta atttgattat
     6301 ggtcgcagta accttcgtgc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcggacaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggcgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttgttaaa tatccatact caagatcttc atctgttgaa
     9601 aaggattcat ttcccaagga gattgatgcc acattactat taacaataaa taatgagcaa
     9661 atgctatctg aaagaacagc aagaaaattc ttcaccgctt taatcctatc cgacgtgtcg
     9721 gtatctaaag caagcaaatt cttgttatca ttaaatataa actcgggtaa atgcagtatg
     9781 cattttttgt cattcaagca actatcaatg aattgttttt taaaaaacag aaagctccat
     9841 gacataaaac tacccacacc ctgaattttg ttgtcagatt gcagatcaac gccaaataat
     9901 gtctttacat cttccctgag attatagaat gccgtatcaa caagagcctc gatcgtttgt
     9961 ttatcaacaa ttttaataaa ctcttcgtat acttgctttt gcgtcttatg gctgcccagc
    10021 gcccatgaat actcatgttc ttttaaaacc agtatggctc ttaacagaca atatccatcg
    10081 ccatctactg gagcgtattt taatccatat gttttattaa tttcactctg aataaaagat
    10141 atgccagaca aatcaatacc agtatctaaa tcagatattc ccggcatatt tatctgttct
    10201 ggctggatac tcatattttc attatctaaa caacactcag aaaaaacatc atcacacgca
    10261 ttaatgtttt caatatattc cataaagcca taccaataat aacctttaaa atctgtgata
    10321 gactctgaac ctctgcccgg caaggacact ctaacaatat caacatcttg aacatattgt
    10381 atagtatatg taatacctcc tgaaacccct gaataaattt gtgtatcatt agtacgcaat
    10441 gttctcataa ataatgatag atcattatca ttctgcattt cctcaaattc tttttctagt
    10501 gattcacatt ccagacatgg agtatcctct tcatttactt tacgtctgag ttcattgtac
    10561 aaccgcataa aacacaccag tgtgttttca aaaagcgtga ttgaatatgt ttgatttttt
    10621 tcgtactcat tttttatatc gttccaggcc tgtttcaaat aattatttgc agtgcaacta
    10681 attccatgta tattactacc catatatagc gtctataaaa tttaataaat aatgacgccc
    10741 tagttaaact taaagtgcct ggttcaacta tcaaaaatcg ctcacccttt ttcacctgtt
    10801 taaaatatgt tcagcaaccc atcttgatgg cgacctcctc tccgcgatga tttcaataac
    10861 atattctgtg ttggcatatg aaattttgag gattacccta cacttatagg agttacctta
    10921 caggggttcc ttcaatttgt gttgatttac gcgagataac gctatggaat taaaggatta
    10981 ttacgccatc atgggcgtga aaccgacgga cgatctcaag acaatcaaga ccgcctatcg
    11041 tcgactggcc cgtaaatacc atcctgatgt cagcaaagaa ccggatgccg aagcccgctt
    11101 caaagaggtc gctgaagcct gggaagtgtt aagtgatgaa caacgtcgcg ctgagtatga
    11161 tcagatgtgg caacatcgca acgatccgca atttaaccgt cagttccatc atggcgacgg
    11221 tcagagtttt aatgccgaag attttgacga tatcttctcg tcaattttcg gtcagcatgc
    11281 ccgccagagc cgtcaacgcc ccgccgcacg tggccacgat attgaaatcg aagtggcggt
    11341 attcctcgaa gaaacgctta ctgagcataa gcgtaccatc agctataacc tgccggttta
    11401 taacgccttt ggcatgatcg aacaggaaat cccgaaaacg ctgaatgtga agatcccggc
    11461 aggcgtcggc aatggtcaac gcatccgcct gaaaggccag gggacgccgg gcgaaaacgg
    11521 cggtccaaat ggcgatttgt ggctggtgat tcatattgcg ccacatccgc tgttcgatat
    11581 tgtcggccag gatctggaaa ttgtggtgcc ggttagcccg tgggaagcgg cgctgggtac
    11641 taaagtcacc gttccaacac tgaaagaaag cattttgctg actatcccgc caggcagcca
    11701 ggccgggcaa cgattgcgcg ttaaaggcaa aggtctggtg agcaaaaaac agaccggcga
    11761 tctgtatgcg gtactgaaaa tcgtgatgcc gccgaaaccg gatgaaaaca ctgccgcgct
    11821 gtggcagcaa ctggcagacg cccagtcgtc ttttgatcca cgtaaagatt gggggaaagc
    11881 ataatggcta atgttacggt gacttttact attaccgaat tttgcctgca taccggcatc
    11941 tctgaagagg agttgaatga aattgtcggt ttgggggtgg ttgaaccgcg tgagatccag
    12001 gaaacaacct gggtatttga cgaccatgcc gccattgtgg tgcaacgcgc ggtacgcctg
    12061 cgtcatgaac tggctctgga ctggccgggg atcgcggtgg cgctgacgtt aatggatgat
    12121 attgcgcacc tgaagcagga aaaccgcctg ctacgccagc ggctttcccg gtttgtagct
    12181 catccgtgat gtgtgtttgc cggatgtgat gacgcatccg gcaactgcgc cataatgcct
    12241 gatgcgacgc taaacgcgtc ttttcaggcg cataaacgac gttatctgtt ttggtggtcg
    12301 cactccacta acaccagcaa taactggctt agtgccgcgt aaaaaccaaa gctgtcatac
    12361 tgtcgacaac gcgcagcaaa ctctggtaac cattgccgca gcgccgtcag tgttttttgc
    12421 cgcaaactgt cgattcttcg cgcaggaacg gtcccctctc ccagcgaaaa atgcagataa
    12481 ctcaacaact cgagatagat cgccagatga tctgccggtt cgttgaaatt gccactggtt
    12541 tccatccctg cctcaactaa caagcgttta atctcttgct cgtcctgttt gtaggccgat
    12601 gcatacggca gcgccgcttg tttgtcggtc atcagaaaca ggccgcaaaa gtcagcggcc
    12661 agttccagac gggcatcgtc acgtactgtc agcgtg
//
LOCUS       DAJTSA010000104        12667 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000104 DAJTSA010000000
VERSION     DAJTSA010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12667
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.104"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(52..531)
                     /gene="xdhC"
                     /locus_tag="OO369_003276"
     CDS             complement(52..531)
                     /gene="xdhC"
                     /locus_tag="OO369_003276"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCR5983438.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(528..1406)
                     /gene="xdhB"
                     /locus_tag="OO369_003277"
     CDS             complement(528..1406)
                     /gene="xdhB"
                     /locus_tag="OO369_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR5983439.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1417..3714)
                     /gene="xdhA"
                     /locus_tag="OO369_003278"
     CDS             complement(1417..3714)
                     /gene="xdhA"
                     /locus_tag="OO369_003278"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCR5983440.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            4129..4884
                     /locus_tag="OO369_003279"
     CDS             4129..4884
                     /locus_tag="OO369_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR5983441.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4963..5036
                     /locus_tag="OO369_003280"
     tRNA            4963..5036
                     /locus_tag="OO369_003280"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4995..4997,aa:Gly,seq:ccc)
     gene            5171..6781
                     /locus_tag="OO369_003281"
     CDS             5171..6781
                     /locus_tag="OO369_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5983442.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6813..7420
                     /locus_tag="OO369_003282"
                     /pseudo
     CDS             6813..7420
                     /locus_tag="OO369_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8351..9097
                     /locus_tag="OO369_003283"
     CDS             8351..9097
                     /locus_tag="OO369_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5983443.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
     gene            9395..9886
                     /locus_tag="OO369_003284"
     CDS             9395..9886
                     /locus_tag="OO369_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983444.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRAEAERIAKELASQKE
                     RTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            9970..10959
                     /locus_tag="OO369_003285"
     CDS             9970..10959
                     /locus_tag="OO369_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983445.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            11621..12166
                     /locus_tag="OO369_003286"
                     /pseudo
     CDS             11621..12166
                     /locus_tag="OO369_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12198..>12667)
                     /locus_tag="OO369_003287"
     CDS             complement(12198..>12667)
                     /locus_tag="OO369_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5983446.1"
                     /translation="GDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLPCDAL
                     QMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENQNLA"
BASE COUNT         3251 a         3144 c         3148 g         3124 t
ORIGIN      
        1 ttaaacacat aacctcagtg aatccggcct gaattcaggc cggatatcct tttacttcgt
       61 tttctcgcaa tccagaactg tatttacaat catctgatac cccgtgcagc gacaaagatt
      121 tcccgccagt ccgcgacgaa tttccgtaat ggttaatggc ttctcgcgtg gtttcgccag
      181 cattgccgtg gtagccataa tcaggccagg cgtacaaaac ccgcactgca ctgtgccgga
      241 tttcgcataa gcctgctgaa catgagaaag ttttccgcct ttcgcttcac cttccagcgt
      301 gcggatctct tttccttcag cccaggcggc aaggtataag caactgtcta ttgctgtgcc
      361 gtcgaccaac accgtacagg caccacattc acccacgcag cacccttgtt tgacacttag
      421 cagtccttgt tcgcggagta attccgagag cggcgtgcct ggtgcggcgt gaagctgaaa
      481 aggcatcccg ttaatggtgc attcggtggt aattgtttcg ctgtgattca ttgcaatttt
      541 ccccccgccg cggcgacggc ttcgctaatc acttttttgg tcatcgtctg gatgagatgc
      601 agacgaaact ctttactggc ccgccatgaa gaacgcgggg cgacatcttg caggacagat
      661 tcgctgatag cttccagcgt ttgcaggttt aatggcgcat tttgtgcagt ctgttcggca
      721 tgttggcagc gaatcggcgt tggcgcggca acaccaaatg ccaggcgtaa ttcgctgaaa
      781 ttgccgttat ccagtcggca atgtgcggcg cagccaatcg ttgaaatatc cattgcgtcg
      841 cgcatggcat atttaaaatg cgcgctgccc gcgtgttctt tcggctgtgg cggaaaatga
      901 aaggcgacga ggatttcgtc atgctcaaga gacactttgc ccggcccggt gtgaaagcca
      961 ttaatcggga cgaaacgaac accgcgcggg gagtggatct ccagtttcgc gtcataaatt
     1021 agcgttggcg tggcagaatc tgcgctggtg gcaccgttgc aaatatttcc accgtaggta
     1081 gcgacgttac ggatctgcgg tccagcaatg aacgtggccg cagcacataa cgccgggaga
     1141 tgacgttgag ttataggatc ttctattagc tgggtaaatg tcgttgcaga gccgatacgt
     1201 agcgagccat cttccgccag cgtaattccc cgcagctccg ccagattatg aatatcaaca
     1261 atatggcgat aacggtcatt gtgatggtgg agctgaatca gtacgtcagt gccaccggcg
     1321 agcagtttgg cctgcgggtt gtcagccagc aggttgatgg catcggcaag ggttgctgcg
     1381 cgatggtaag aagcaaaatc aaacatgatg ttatcctcaa atcaatcctg ccagatggaa
     1441 ctcttcatat aaccgttttg gcgtcagcgg cagtgtattg attgcaacac cggtagccat
     1501 cttcaccgcg ttacgaatag cagcggcaac aggaattatg ggtggctcac ccagtgactt
     1561 atgtccgtat gcggattgcg gctcattgat ttcgacgaac gcgctttcca gttgtggcag
     1621 atccggcatg gtcggcattt tgtaatccag cagattgggg ttacggacca cgccgctttt
     1681 agcatcgatg atcatctctt caaatagcgc ccagccaatg cccattccca ttccgccgtg
     1741 tacctgacct tctgccagca gtggattaag aatatgccct gaatcatgaa cgttgaggat
     1801 gcggttgatg gtgactttgc acagcgcaat atcgaccgtc agatcaacaa aggtacagcc
     1861 aaacgccggt gggttagtgg tggttttgat ggagctttca gcagagagct gcccgccgcg
     1921 ttcagggtgg tagaaagcgt ccatcgccaa atcttttaac gacattaacg gctcttccgg
     1981 tcgttcaacc agcacgatat ggccttttat cagggtcaaa ttcatcgctg actgatgtag
     2041 catgactgcg gcgtgagcga tgattttctc ttttaataat agtgccgcac tgcgcagcgc
     2101 aggcgcggca acatagctct ggcgtgaggc aaatgcgccg ggatcgaacg gcgtaacgtc
     2161 ggtatcttgt gttgaaataa cgcgaacgtc gctgaccgga acccccacgg tttctgccac
     2221 catttgcgag aagacggtgt cggcaccctg accgatttcc gtcgcgccgc tttgcacgtt
     2281 gatggttcca tcctgattca tcagaaggcg cgcgcctgct atttctacgc cgacaggcca
     2341 ggtgttagag gtgtagctaa aacaggcgac gccaacgccg cggcgcaaat tgccttgctg
     2401 gttctggcat tctgcacggc gtttttccca ttcaaagatt ttccggcctt tttcaagaca
     2461 ctccggcaac cctgcgctgc aaatacgttt gcccgtgagc ggattagcat ctccttcgcg
     2521 ggcggcgttg cgtaaacgaa tttcaacagg atcaatacct aacgctgtcg cggcgtcatc
     2581 aagcatagac tcaacggcaa atacgacttg tggcgcgcca taaccacgca tcgcaccagc
     2641 cgaggggagg ttggtatagc aggtctttga actgtaagcg taggcacaac gaggataaag
     2701 gtaagcgact ttattccccc ccgcagaagc gatggagtgc ccgtgagatg cataagcgcc
     2761 ggtgttagac agaacatcca gactataacc tttcaaggtt ccgtcgcggt tcacgcccat
     2821 ttgcccgtca atagtaaaag cgtggcgggt acgggttgcg aggaaacact cttcacggct
     2881 aagggaaact ttcaccggaa tgccgccaag cttgctggtc aggaatgccg ccattggctc
     2941 ttccagtaca tcctgtttat taccaaaacc gccaccgaca aacggtttga tgactcgtac
     3001 gcatgaccag ggaatatcca gcgcctgacc aaccacgcgg cgaacaatgt gcgggatctg
     3061 ggtgctggaa acgatggtaa ttcgcgagtc atcctccatc cacgccagcg atgttacgct
     3121 ttccatgtga caatgttgaa taacgggagt ctgatagtgc ccctgtacct ggtagtcggc
     3181 ggcatcgatt gtttgttgga cattgcccgt cgacatcgtg ctttgtttca gtaaattgcc
     3241 gccgttatgg attggtgcag cgtcttctgt cagcgccgct tctggcgtgg tgataacggg
     3301 taattcttgc cactcaatgc tgaccaattg cgccgctttt tctgccgtga gttcatcgcg
     3361 ggccacgacg atggcaacgg cgtcaccatg atgacgaaca tggcgagtta gcagtgcgcg
     3421 atcggcggta tcgcgcttgt tttcgtcaag tgtccaggca tgcccagctg tagcgaatgg
     3481 aatatcaggc acatcttccc aagtaaaaat cgccagtacg cccggtaaac ttctggcttg
     3541 ttcatcatta atacttacgg cataaccatg tgcgataggg ctacgtacat atttcgcgta
     3601 acacatgccc gccataacat aatcgtcagt atatcgtgcc cgcccggtga ccttagcaat
     3661 ggcatcgacg cgcatgcatg attcacccgt agcggttgct tcccgcgctt ccatagaaat
     3721 cccctcgatt tgaacatttg gttcttatgg caaagattta gtctaagcag gcaagattta
     3781 cgccagaaac gacgataagc gttaggggag gaaggaagtg tgatgacgtt aaaaaaacac
     3841 agatatgata cgaaacagga ctctgaatag ggacagtgat acgtatcaat atgagtggcg
     3901 ataaaatcaa tgtgataaat caggattatt acgcatcagg atgggaaata gggtaaaaag
     3961 tcaggaactt cgatacctct acagcgaata ttttgctgat gatacttccc tttgccaaat
     4021 gaaattaacc tctttctcta ttaccacgtt tttccagaag caagagattg ggtatcaaga
     4081 ggctggctgc tatgatatgg cgtcttgttt tttaagcgag gaaagatttt gagtgcggga
     4141 cgcctgaata aaaaatctct gggtatcgtg atgtttttat cggttggact gctattggcg
     4201 ggctgttcgg gtagcaaatc atccgataca ggaacgtatt ccggctccgt ttacaccgtg
     4261 aaacgggggg atacgctata tcgtatttcg cgcaccacgg gaaccagcgt aaaagagctg
     4321 gcgcgactga acggcatttc ccccccttac accattgaag ttggtcagaa actaaaactg
     4381 ggtggggcga aaagtagcag tagtacacgt aaatcaaccg ccaaatcaac gactaaaacc
     4441 gcatcggtta caccgtcatc agcggtaccg aaatcttcct ggccgccagt agggcaacgt
     4501 tgttggttat ggccaacgac agggaaagtt atcatgccgt attcgacagc agatggcggc
     4561 aataaaggga ttgatatctc agctccacgg ggtacaccta tttacgccgc gggtgcagga
     4621 aaggtggtgt atgtgggcaa ccagctgcgt ggctacggta atctcatcat gattaaacac
     4681 agtgaagatt acattacggc ttacgcccat aatgacacga tgctggtaaa taatgggcaa
     4741 agcgtgaagg ctgggcaaaa aatcgccact atggggagca cggatgcggc atctgttcgc
     4801 ctgcatttcc agattcgtta ccgtgcaacg gcaattgatc cgctacgtta cttgccgccg
     4861 cagggcagca agccaaaatg ctgatggcga attaatcagc agtcagcagc gcggcacttg
     4921 ctaaggagag cgtaaggttt ataatgcctt acgcatctcg aagcgggcgt agttcaatgg
     4981 tagaacgaga gcttcccaag ctctatacga gggttcgatt cccttcgccc gctccatgac
     5041 acttcttaaa gtctactaac ttactgaatc ctaagggata cggcttaacg ccttccgata
     5101 gttttactca ctgtaaccaa cactgtgact aactttgttg aagtagtgag cagacctaag
     5161 gtctcagtac atgctcaagt caagaaccta cctttatcag cgtaatggtg tcttttacat
     5221 ccgtcttagg atgaagacca ctagtcgcct gaccgcttcg cttccttctc acaaccgtta
     5281 taaactagcg tcagtatctt tacggaccaa ggatagacgt accgctatgg ctcactcacg
     5341 gcacatcaag tcagcactta aagcaatcca tgcagacaac cctaacgcct cttatgagga
     5401 gctacgggag cacctgaaga ccattgtcga gtgggaactt agtgtcagcc gtgatgacct
     5461 gaacgacccg gagtcctacc agctttacgt tgaccagtac gatgacatta agtccaacct
     5521 tcgggaagct gtagcgaccg agcgtttgac tgtagaccag catcgctata ttaatgacgt
     5581 tatcggtgtg cttaaggcct gtcaggatag actcaatggc gatagctctg gcctattgtc
     5641 ttaccttgag cctgagactg gcagtctaag accgtccgtc tccctatctg tattggcgga
     5701 acctgaagtc cctgaaccta aagcactgac cctcgcgtct ctcattgagc agtacgagca
     5761 ggagaacgcc cagaactgga agccagcgac cctaagcgaa aaccgagcgt cacactctac
     5821 gctaatcgag atattcgact atctggatat tcaggatgtc ggcaaggcga cccgtgcaga
     5881 tatgctcaga gttcgtgaag tcctccagca gctaccgaag aaccgcaagc aacgctttaa
     5941 gtctatgccg ctgtctgact tgctgaaccg ggagtctaag actgactgtc tggacgtcgt
     6001 taccattaac aataaatatc ttatcaagat ggctgctgtg tttaagtggg cggtacgcaa
     6061 cgacttgata gctaagaacc tgaccgaagg tcttgagctg aaagtccctc agcgtaaagc
     6121 ctccgacgct cgtgatgcgt tcagtcctga gcaggtaggg caactactgg tcgcagctaa
     6181 agcgtactcg cagaagacct caggtaagcc ataccattat tacgtcaccg ctctggctgc
     6241 gattacaggt gcgagactta acgaggtggc ccaacttcag gttaaagatg tcaggaccac
     6301 tgaggctggg accgttttta tccatatcaa cgaagacgat agcagcctgc cgggtaagag
     6361 tatcaagaac gcccacagtg accgctgtgt cccgttggta gatggtgctt atggttttgt
     6421 attggctgac tttatgtcac tcgtggaaga ccgtagaaag actgagggtg ataatgctat
     6481 ggtgttcaat ggcctcaagc taatgaagaa cggctacggt gagcaggtta gcaagtggtt
     6541 taaccgaacg ttgctgccca aagtcctcgc tgaccgtagc ggcttagctt ttcactcgtt
     6601 tagacataca gttgcgaccc agttaaaaca acatggggtc gagttagcgt atgctcaggc
     6661 gattatgggt catagctcag ggtctataac ttacgaccgt tacgcgaaag aggtcgaggt
     6721 ggaaacgctg aaagaaaaat tagcggagtc attgtcagta aaaaaaatag acggaaagta
     6781 atcgcatagg gatagcggaa tgtgaggacg taatggtcaa agaattttta gttagcaaag
     6841 aaaataagcc ttcatatgaa tgtatgaata ctgctgaaac ttgtttgggg ctggctggaa
     6901 tggcttcgga agattggcaa cgtgagcatc attgccgcac aggtataata tttactgctt
     6961 ttgctattga ggcaatgttc atcttctata gaaaacaggt tgaccccggc tacgataaga
     7021 cgcagaaaga gtgcagaaag acaatgcaca agaatacgtt aaaattgtgc ggtataaaca
     7081 attatatggg gacaaaacct taccaaatta tcaaagagtg tcttgaggta agagacgcta
     7141 ttgctcacgg cgattcgtat acttcgagct ttaatttctc ggcggatcat ttggataacc
     7201 aagacgatat tgcaaaagtg tcctggcggt aaactctgaa caatttcggg gattaactgt
     7261 tgagcgcctt gcaaaataca ttgaaatggc aaagcatatt gaccatgaga tttctgataa
     7321 tggttacagg ccttctgaag gttaccttcc agcagccgag cgcagaagtc tgatgttagc
     7381 ttttggcgtc agtggtgtgt caatgtggcc cagtgcttga gcgataatac acctcactat
     7441 agaggaatgg acccttaact atcactatag gggagagacc ctaagtcccc accttaatag
     7501 cagtcacgac ccatacacac attcattaag tctcaggctt ccggtagcgg accgattgga
     7561 aaccatccgc agacgcctag tgttaaatca ccttggcctg agaccattcc ttttaacgta
     7621 agctatcttc ccttttgaca ggcggggagc agtagaccac atccgacttt aagttggctc
     7681 tgtgataatc gctgcgcgga taactgaaga accaagacac cattactgag gtctgccatc
     7741 catttgtgga caggcggaaa ctcaaagagg ttagatcagt ggtgtctgag gttggagctt
     7801 tgagtcttga ccagcaggct ggaggagacc atgagtttat cttgttatgg attgtgttat
     7861 cacaattcca ataagaagaa ctttaagtcc agcctccact cccgccaacc cgaagtaaaa
     7921 ttatggtcgc tatgatgtgg tctccatgct tcgggtctta ctggaagtac tgaacacttt
     7981 cgcgtcaagt tctccgaagt tcattcctgt ttgactaaac tatcactata gggaccatat
     8041 ctcgcctctg acctcctgag agacggcaag cgagaaaccg tggtccacgc tagcaagcta
     8101 gccaccagcc taaggcaaga cagcctctgg tcagcctgtg tgttctttgg tctcgtgact
     8161 taagtctatc accacacacg ctcggacatg ctgtcgaacc ttcatctcta tggtcatcgc
     8221 agaccactaa ccccaattac ctcaagtcat tctgtttgcg tcctgttgcc tgctggtctc
     8281 cctttacgtg ggggcttgag taggtcagtg gggtgcgact tcagggtgca aacaaaagga
     8341 gtacacgtaa atgtcccaac aagaaatcag catcattaac ctcgaccagc ttgtgtccat
     8401 gacctcagtt gaaattgctg agctgacagg aaaagaacat aaacacgttt tacgagatat
     8461 tcgtaatatg gtcgaagagc ttaacggggc caaaactgag cactgctcaa ctttgagcag
     8521 cgagcttaac gggtccaaat ttgggctcgt tggcgaggaa gtatacaagg acgcgaaagg
     8581 tgagagccga acaatgtatc gtctcgaccg taagcatact ttcattctgg tcgctggcta
     8641 ttccgttcac ctccgcgcca agtgttacga ccatattcag acgttagagc gtcgagtcct
     8701 tcaactggaa gaccagaaga agcgagcagc cattcagtcc gctaaccgcc gaggcgtgac
     8761 ttggggcgac tactgcaaaa cctatggtct gcccgctcag aagctaatga ccgcacttct
     8821 ccagcatcgt ggcctctttc gtaagaaccc aatcagtaac gagtggtctg taaatccgaa
     8881 gtattccgac tgctttcgta tcattaagcc gtcagaccag aagttctcag caggtgggta
     8941 taacttccgg ttcaacgcta aggggctgga ggtcttcggt aagcctgaga tggttgacaa
     9001 gatgcgaggc atccttattg cattcactgg caccgaccag cagaagcaag agcacctcct
     9061 gaagctggca caatccggta aggtggaggg aatctaagat gatgattgac ccgcgcacac
     9121 ctgaggggcg catgactctc cgttaccgtg gctaccggac tgaagtcctc ctcagagagc
     9181 ttggtctaga cccggaagat gagacccgcc agcaccaatc ccgagacgag ctaatagccc
     9241 aactggtcgc tatgaaactg ccgcttaacc gttagtagct cacctactca atacctaatt
     9301 taaacacctt ttgagatgac cgatgcgggg agacccgtag gccttactgt gcctgcgata
     9361 ccgatagtct caactctcaa gaggagaatt acctatgatg ttcaacaaca acaactggaa
     9421 gctgtcagtc accgacatca acctttatga aaacactgtg agtctggacg gtcagtccta
     9481 tccgttgtcg ctcgctatca agaccctcat tccgggctac ctgtcaggct taccgtccac
     9541 cagccgcgag gcgatggagt tactggaagc tctggctgaa gctggcgtga ccattggtaa
     9601 cttcttcagt aatgacctga tgaccgccta ccaacgccgc cagatgaaca aacgagctga
     9661 agctgagcgt atcgctaaag agctagcgtc tcagaaagag cgtacccgcg aaatgttcat
     9721 gaccgaagac gaatggcaaa aagagctaca gcgccgcgaa caggtcaaag ctgaacgccg
     9781 gacctatggt gaaaacctcc gcagcgctac tcattccgct ggtcgctcac gtgccgctat
     9841 cgtcgctgac cttgagtcag gcggtaactg gatggacagc ttgtaagcaa gcgtccctca
     9901 tctgagaaca cacggtccct gactgcctgt tagggacttc aagtatttga ctcaaggaga
     9961 ttaattataa tgaatatgac taaaaacgct ttccagtttg gcattgagcc agtacgcatc
    10021 accgataccg acaacattca ggtcaatgaa ggtctgccga ctaacgccga ccctcaggtc
    10081 tatgccctcc agttagctaa gactgttaag gctatgctga acggtgtact taaagacgct
    10141 caagataaca tcccgttccc tgtagaagtc ctgccgaccc gtaacagctt accgactcca
    10201 atcattgctc acactctggc tgaccgctct gtgctggtcc ctgtccgtgg tggtaagcga
    10261 cctgaagttg tgaccgctcc gtctggcact gaaatcaccg ttgagccgat tgaacaggcg
    10321 atcctcgtgt ctcaccaaac aaagctttgg gaccagaagt cgaccactgg tttcactcag
    10381 gggacgctcc agcaggacgc actgaacatc tgcgataacg tgattcgtac cattaactct
    10441 aagatggttg acgtgctgga gtcctcaaag ctgctgaaga ccgtagaact tccggccctg
    10501 actggtagcc tgaccgctaa ggctgacgct atcatggatg ccctgtacga gaacacggag
    10561 tcttctttcg gttctgaggt ttctgactac ggcattatcg cccatgagtc acacctcaag
    10621 gctctctccc gtctggctgc taagcagggc ttcggtggtg aagacgctat cgtcgatatg
    10681 ctggggactg acgtcgccta ttacaacggt gaagaccgtg gcgtgttcat gatggctaag
    10741 cgttttacgg ctctgagctt cggttgcttc cgtcacgatg gcgagagcat tactgtggtc
    10801 ctttcacgtg atggagactc ccagtctcac gaccttgaga ttctcggcaa ggtgtttgtt
    10861 gtagctgaag ctgctacgac catcaagatg ggtactggct cggctactgc tgtcctgcct
    10921 gtagtcaaac gcctgagctt cactaaagaa gctaactaac aataacgact attggtcaga
    10981 cttccgaact ggatgtctgg cctttatttt gtactagtaa tctagtttgt gaatacagaa
    11041 agtaggaggg ccaaatggac ctacacaaag tctatctcgt ggtcttcctg ctaaagctgg
    11101 gactggtggt ctggctatgg gctattagac gatgagtttt gactgaaaaa tgtgagacct
    11161 catctaatat aaagagcgag ccaaaagccc ccatagcctg acctgagcca gaccaaaagg
    11221 gccgggtagt cagaccgtga gttctattcc tttagaggga atattacgtg cttcaagtct
    11281 tgaccttcta tctctaaatg tgtgactatc actacaacca actggattca cctgtcttta
    11341 agaaacaata ggttagctgg ttgtcttcga tttgtcgtag gctcccttcg cccgctccaa
    11401 tttatcttca cctgatcaat ctgccatttt tgctgtatct gattttctat attatcttaa
    11461 ttcaatcact tcaggctttt cgcaatcgct gtaacacttc cccatttttt tgtaaataaa
    11521 aagcgtttac gcaatgcaat ccttttgtaa caactcaggt aaaatacctt attttcaagt
    11581 tgttgaataa gtggttatct acgcttttaa caaacgattg atggattatt ttatgaaagg
    11641 gaaatctgca ttaactcttc tgctcgctgg tatattttcg tgcggcacat gccaggcgac
    11701 agaggcagaa gtgacaagcg aatctgtatt taacattctt aattccaggg gggcagcaac
    11761 agataaaagt tatttatcgt taaatccaga taagtatcct aattatcgtc ttctgattca
    11821 ctcagccaaa ttaaaaaatg aaataaagag tcactatacc aaagacgaaa ctcaaggatt
    11881 gctaacatta acagaaaaca cacgaaagtt aacgttgacg gagaaaccct ggggaacgtt
    11941 tattcttgct tctactttcg aagatgacaa aacagcggca gaaacccact atgatgctgt
    12001 ctggctgagg gatagtttat ggggctatat ggcattagta tcagaccaag gaaatagtgt
    12061 agccgcaaaa aaggttctgc tcaccctgtg gggttatatg tctacgcccg accagattaa
    12121 gcggatgcag gacatcatta gcaacccaaa acgactggat ggaattctga tcctacccac
    12181 gtaatatgga cacaggccta agcgaggttc tggttttcaa attgttccgg actgaggccg
    12241 ccacaccaac tgtgccgccg ccaccgattg taatcacatt cgatataatt aaacaccgtt
    12301 gcccgcatta tttcccggct gataaagtgt tctccatgga tacattccac tttcagcgaa
    12361 tgaaagaagc tttccacgca ggcattatcg tagcagcaac cttttgcgct catacttcca
    12421 cgcagattat gccgtttcag ttgcgcctga taatctgctg aacagtactg gcctccacgg
    12481 tccgtgtgaa cgataacgtt ccggggcctc ttacgccgcc acagcgccat ctgcagggca
    12541 tcgcagggca gttgcgccgt catgcgtggc gacattgacc agccaataac ggcacgtgac
    12601 cacaggtcaa tgaccactgc cagatacagc cagccttcat ctgtacgtaa gtacgtgatg
    12661 tctcctg
//
LOCUS       DAJTSA010000105        12662 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000105 DAJTSA010000000
VERSION     DAJTSA010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.105"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="OO369_003288"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="OO369_003288"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5983447.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="OO369_003289"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="OO369_003289"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR5983448.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDELLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="OO369_003290"
     CDS             3115..3399
                     /locus_tag="OO369_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5983449.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="OO369_003291"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="OO369_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR5983450.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="OO369_003292"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="OO369_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR5983451.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="OO369_003293"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="OO369_003293"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5983452.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8449..9330
                     /gene="yddG"
                     /locus_tag="OO369_003294"
     CDS             8449..9330
                     /gene="yddG"
                     /locus_tag="OO369_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR5983453.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9585..10685
                     /gene="ompC"
                     /locus_tag="OO369_003295"
     CDS             9585..10685
                     /gene="ompC"
                     /locus_tag="OO369_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5983454.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            10849..11334
                     /locus_tag="OO369_003296"
     CDS             10849..11334
                     /locus_tag="OO369_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5983455.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            11444..12433
                     /locus_tag="OO369_003297"
     CDS             11444..12433
                     /locus_tag="OO369_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5983456.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         3340 a         3128 c         2941 g         3253 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg aggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc tgttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ctataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     8461 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     8521 cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     8581 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
     8641 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct atctggcgct ttccttaggg
     8701 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     8761 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     8821 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat
     8881 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
     8941 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     9001 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     9061 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     9121 accttaggat ttgcttatac tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9181 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
     9241 agcgccccgc tgtcgttctc gttctggcaa ggcgcactaa tggtctgcgg cggttccctg
     9301 ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt
     9361 aatcagataa attccgacaa tattaaaaaa taaatttcaa ataccagtaa ttcaccttat
     9421 atctcatttc gacttcgatc acatatcgat aaataatatt attctatttt tctcattgtt
     9481 tttaatcacc attctatttt aaaaacagta tgtcatttat aaatgacgcc gtttaaaatt
     9541 cgtttagaaa atacgtttaa cgataattaa aaggattctg ttaaatgaaa ttaaaaatag
     9601 ttgcggtggt tgtaactggt ttgttagctg cgaacgtagc acacgctgcc gaagtctata
     9661 acaaggatgg taataaactc gacctttatg gcaaggttac cgctctacgt tattttactg
     9721 atgataagcg tgacgatggt gataaaactt atgcccgtct cggctttaaa ggagaaacgc
     9781 aaatcaatga tcaaatgatt ggttttggtc actgggaata tgattttaaa ggctataacg
     9841 atgaagccaa cggctcgcgc ggcaacaaga cccgtctggc ctatgctggt ttaaaaatta
     9901 gtgaatttgg ctctctggac tatggtcgta actacggtgt cggctacgac attggttcat
     9961 ggaccgatat gttgccagaa tttggtggcg atacctggag tcagaaagat gtcttcatga
    10021 cataccgtac caccggtcta gcaacctatc gcaactacga tttctttggc ttaattgaag
    10081 ggctgaactt tgccgcgcaa tatcaaggca aaaataaacg tactgacaac agtcatcttt
    10141 atggtgctga cgacacgcgt gccaacggtg acggtttcgg tatctcctca acttatgttt
    10201 atgatggctt tggtatcggt gcggtatata ccaaatccga tcggacaaat gcgcaggaaa
    10261 gagccgcagc taatcctctc aatgcctccg gtaagaatgc agaactatgg gccacaggta
    10321 taaagtatga tgccaacaac atctactttg cagctaatta cgatgaaaca ttaaacatga
    10381 ccacctatgg ggatggttat atctctaaca aagcacaaag ttttgaagtg gtggcgcaat
    10441 atcaattcga cttcggcttg cgcccatcac tcgcttacct gaaatcgaaa ggcagagatc
    10501 tgggccgcta cgccgaccag gacatgattg agtatatcga cgttggtgcg acgtatttct
    10561 tcaacaaaaa tatgtcgacc tatgttgatt ataaaatcaa cctgattgat gaaagcgact
    10621 ttacccgtgc cgtagatatt cgcaccgata acatcgtcgc tacgggcatt acctatcagt
    10681 tctaacgtcc tctcctgacc agcccttctc tgggctggtt tttgatttaa gtgctgctgt
    10741 atgactcctt aagtaaaaat gagcaattct tgatatttct ggtcattcac ctcaattaat
    10801 attattccat atcctccggg gacatcttac tataatattt attgatgagt gattatgacc
    10861 aatattaata cagcttgtgt aaaaaataat gccagttatc aacttaataa cgcattacct
    10921 aacaaagaaa ccatctccag caatttttgt gaacgactag aacaatgggg taataaatcg
    10981 gttaataatg gtgaagaaag agcaattgcc gtggagcgaa ttaaagaagc ttacaattcg
    11041 aacatggcat ctcttgattt gtcatatctt gatttgagtg aattaccgcc tatcccctcg
    11101 acagttaata cgttaaatct ggaaaacaat tgtctaactt gtcttgactt agctgacaat
    11161 gccagtctcg ttaatatcaa cctcagcgtt aataaaatta acacgataac ctttccaaaa
    11221 gaaccaaaac tggaaaacat ttatattaat cataacaatc tggaaagttt agatttaaaa
    11281 aatcagcatt cattggttaa tctggaagcg caaaataata gcctaaaaaa atgatatttc
    11341 tgatagttat aaactgaaat ttcttaatct tgattataac aagctagcat cgctggatgt
    11401 ctcccggcaa gaatctctga ttgagttaag tgcccatcac aatatgatca ctgaccttat
    11461 attacacaat catcccagga tgaaaacaat cactttaaac gacaaccata ttgcacattt
    11521 aaacgccaaa aacactacaa aactggaata tttaaactta agcaataaca atttactgcc
    11581 aaccaatgac attgatcaac taatatcatc aaaacatctt tggcatgtat tagttaacgg
    11641 catcaacaat gatccacttg cacaaatgca gtactggact gcagtaagaa atataattga
    11701 tgacactaat gaagtgacca ttgatttatc aggacttaat ttaaccactc aaccaccagg
    11761 gctgcaaaac ttcacctcta tcaatcttga taataaccaa ctcacacatt ttgatgcaac
    11821 caactacgat agactcgtaa agctaagtct gaatagtaat gctcttgagt caataaattt
    11881 tcctcaaggc agaaatgtaa gtattacaca tatatctatg aataataatg ctctcagaaa
    11941 tattgatata gataggcttt catcagttac ttattttagt gcggcacata atcaactaga
    12001 gtttgtgcaa ttagaatctt gcgaatggct gcaatacctg aatctcagcc ataatcaatt
    12061 aactgatatt gttgcaggta ataaaaatga actcttactg ctggatctat cccataataa
    12121 actaacaagt ttacacaatg tcttatttcc caacttgaat acgttactta ttaacaacaa
    12181 tttgctttct gaaattaaaa tattctatag caacttccgc aatgttcaga cattaaacgc
    12241 tgctaacaac cagttgaaat atataaatct tgatttcctg acttatcttc catctatcaa
    12301 aagtttaaga ctggacaata ataaaataac ccacattgat actaataata catccgatat
    12361 tggaacttta ttccccataa taaaacagag caaaaactta aattttttaa atgtttctgg
    12421 gaagaacaat tgacctacta tgcagctcat gttatttaat ttgttttccc cagcacttaa
    12481 gcttaatact ggcctggcaa ttctttcgcc tggtgcattt gaagttcact ctgacggaat
    12541 agatgcggat aacgaattgt ttcactatac tattaaaaaa gcatataccc catataatat
    12601 acacacttac aagacagagg aagttgtaaa ccagaggaat ataaaagtta aaaacatgac
    12661 ct
//
LOCUS       DAJTSA010000106        12649 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000106 DAJTSA010000000
VERSION     DAJTSA010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.106"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1039
                     /locus_tag="OO369_003298"
     CDS             <1..1039
                     /locus_tag="OO369_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5983457.1"
                     /translation="FFPNLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIH
                     GVWFDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMT
                     IYDAEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHF
                     YVIGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTA
                     WPHDGNQHEKGGGEQLQGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFK
                     VFNTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKI
                     PAPIRPIARRT"
     gene            1040..3205
                     /locus_tag="OO369_003299"
     CDS             1040..3205
                     /locus_tag="OO369_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR5983458.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRSKDAR
                     ANAELLLKDADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            3219..4130
                     /locus_tag="OO369_003300"
     CDS             3219..4130
                     /locus_tag="OO369_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HCR5983459.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4130..5425
                     /locus_tag="OO369_003301"
     CDS             4130..5425
                     /locus_tag="OO369_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCR5983460.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5470..5700
                     /locus_tag="OO369_003302"
     CDS             5470..5700
                     /locus_tag="OO369_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983461.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5678..6178
                     /locus_tag="OO369_003303"
     CDS             5678..6178
                     /locus_tag="OO369_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCR5983462.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6178..7596
                     /locus_tag="OO369_003304"
     CDS             6178..7596
                     /locus_tag="OO369_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCR5983463.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7596..8444
                     /locus_tag="OO369_003305"
     CDS             7596..8444
                     /locus_tag="OO369_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983464.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8444..8899
                     /locus_tag="OO369_003306"
     CDS             8444..8899
                     /locus_tag="OO369_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR5983465.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            8902..9594
                     /locus_tag="OO369_003307"
     CDS             8902..9594
                     /locus_tag="OO369_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR5983466.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9604..10935
                     /locus_tag="OO369_003308"
     CDS             9604..10935
                     /locus_tag="OO369_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR5983467.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            10936..>12649
                     /locus_tag="OO369_003309"
     CDS             10936..>12649
                     /locus_tag="OO369_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR5983468.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     DPIEK"
BASE COUNT         3403 a         3129 c         3334 g         2783 t
ORIGIN      
        1 tttctttccg aaccttgttg atcaccttct ggttaagcat cacacggctg atggcgttga
       61 agatggcatt tcaatctgct acttcaagcc atactcgcaa ggccgtgctc gctggcaggg
      121 tgacacaatc cacggcgtgt ggtttgacga agagccacca tacagcattt atggcgaagg
      181 tcttacccgt accaacaaat acgggcaatt ctcaattctg acgtttaccc cgctgatggg
      241 gatgtctgac gttgttacca agttcctgaa gaaccccagc aagtcgcaga aagtggtcaa
      301 catgaccatc tatgacgctg agcactatac cgacgagcag aaagagcaaa tcatcgcatc
      361 atatcccgag catgagagag aggcgcgtgc tcgcggtatt cctacgatgg gtagtggtcg
      421 aatcttccag ataccggaag agacgattaa gtgtcagccg ttcgagtgtc ctgatcactt
      481 ctacgtaatt ggcgggatgg atttcggatg ggatcaccca caggcgcaag ttcagctttg
      541 gtgggataag gaggcagaca caatctacgt ttcacgcgtg tggaaggcga aagaaaaaac
      601 agccgttcag gcgtggggag ccgttaaatc atgggcgcat aaagtgccaa ccgcatggcc
      661 tcatgatgga aaccagcacg agaagggcgg cggtgagcag ctccaagggc agtatgcaga
      721 cgctggattt atgatgttgc aggagcatgc gacatggcct gatggcggta acgctgttga
      781 gccaggcatc actgaattgc gcgacatgat gctcgatggt cgcttcaaag tattcaatac
      841 ctgtgagcca ttctttgagg agttccgcct ctatcaccgt gatgaaaacg ggaaaatcgt
      901 caagcttaac gacgacgtgc tatccgccgt tcgctatgca tacatgatgc gccgcttcgc
      961 aaaaatgatg cgcgacatca aaaaaccaaa agagaaaaag ataccagccc caatcaggcc
     1021 catcgcacgg agaacttaaa tggccgacga aaacagactc aattccattc tgtgtaagtt
     1081 tgacgcagac tggatggcga gcgatgaagc cagaaccgag gcgacaaatg acctgtattt
     1141 tagccgagtg tcgcaatggg atgactggct atcaaactac actaccctgc aatatcgcgg
     1201 acaattcgat gttgttcgcc cggaggtcag gaaactggtc gcagagatgc gccggaaccc
     1261 tgtcgacgtt ctcttccgac ccaaagacgg cgctaatcct gatgcagccg atgtattgat
     1321 ggggatgtat cgtactgata tgcgccataa cacggcaaag attgccgtta acgttggcgt
     1381 tcgtgagcag atagagtcag gcgttggtgc atggcgtctg gtcacgcagt acgaagacaa
     1441 cgatccaaca agcaacaatc aggtaatccg acgcctgcca atccatgaag cctgctcaca
     1501 cgtcatatgg gacgccaaca gcaagcagat ggataagagc gacgctaagc actgcacggt
     1561 gattaacgcc ttgtcgcgca atggctggaa agagttcgca gaggattacg gtattgaccc
     1621 ggataccctg ccatctttcc agaatcctaa cgatacatgg ctattcccgt gggtgtcgaa
     1681 tgatgtcgtc tacgtcgctg agtattacga ggtagaagag gagaaggaga aggtcttcat
     1741 ctaccgcgac ccgctgacag gtgagccggt cagctattac cagcaggata tcaaagacgt
     1801 catcgacgac ctggctaatc gtggattcat taaggtagca gagcgcaagg tgaagcgtcg
     1861 gcgtgtgtat aagtcgatca tcacctgtac gcagatactg aaagaccgcg agaagatagc
     1921 cggagagcat attccaatcg ttccagtgta tggcgaatgg tcattcgctg gtgacaagga
     1981 gtgctacgag ggtgtggtaa ggctgacgaa agacggtcaa cgccttcgta acatgattat
     2041 gtcgttcaac gccgatattg ttgctcggtc accgaagaag aaaccgacct tcttccctga
     2101 gcaaatcgaa ggctacgaat acatgtacgg cggaaatgat gactatccgt actatctgca
     2161 gaacaggact gatgaaaacg gtaacgacct gccgattggt ccaatctcct acatggaaaa
     2221 ccctgaagtg ccgcaagcca acgcttacat gcttgaggct gccaccaacg cagtgaaaga
     2281 agtggctagt cttggcgtgg atgcgcaggc ggcaaatggt caggtcgctt ttgataccgt
     2341 caatcaactg aacatgcggg cagaccttga gacatacgtg tttcaggata acctggctac
     2401 cgcaatgcga cgtgatggcg agatttatgc ctcaatggtc aacgatattt atgacgttcc
     2461 tcgtcatgta acgctgacac ttgaagatgg aagcgagaaa gacgttcaac tctatgcgca
     2521 agttgtcgat taccagtccg gcaatgtggt cacactcaac gacattcgcg gtcgatatga
     2581 gtgctataca gacgttgggc catccttcca gagtatgaag gaacagaacc gcgcagagat
     2641 tcaggagttg ctcaccaagg ttccgcaagg tactccagag ttccagatgc tgatgctgca
     2701 atacttcacg ctgcttgacg gtaaaggcgt cgagatgatg cgagagtacg cgaacaagca
     2761 actggtgatg atggggctga agaaaccaga aacacctgaa gagatggaga tggtgcagca
     2821 ggcacaacaa cagccgcagc agccatcagc agagcaaatt caggcgcagg gtatccttct
     2881 gcaaggtcag gctgaattgc tcaaggcaga gaaccaacag gcgcagattc aggttgaagc
     2941 tgccaaggtt gaagcccaaa accaactcaa cgccgcgaag attgcagaaa tattcaacaa
     3001 tatggacctc gacaagcagg cagaactgcg tgagtacctc aggctcgtag gtcaattcca
     3061 gcaacagcgc agcaaagatg ctcgtgctaa cgctgagctg cttcttaaag atgcagacca
     3121 gactcattca caacgcatgg atttcgcgaa tcttatgcgt caagttcaaa tcccctccgg
     3181 cggagtagcc gagacacctc aataagagag agttaatcat ggaccaaacc accgacattc
     3241 aggcttctga agaattaacc ctgcccggca atcatgcagc ggcatctgct gatggcttag
     3301 ttgtcgataa tgccaacgac agcgcaggtc aggaagaagg ctttgagatt gtccggaaag
     3361 acgatgagaa accaaaacaa gacccggcaa ctaatgctga atttgcccgt cgccgcatcg
     3421 aacgcaaacg ccagcgtgag cttgagcagc agatggaagc ggttaagcgt ggagagttgc
     3481 cggagcacct gcgggtgaac cctgagttac caaaacaacc agaccctaac gattatcttt
     3541 ccgaagatgc actggctaag tacgactatg accagagccg cgcactggct gccttccagc
     3601 aggcaaacag tgaatggcag atcaaggcta tggacgcacg aagccaggct gtcgccgagc
     3661 agggtcgcaa aactcaggag ttcacccagc aatcagcgca atacgtcgag gcggcccgta
     3721 agcactacga ggcagcggaa aagctcaata tccctgacta tcaggagaaa gaggatgcat
     3781 tcatgcaact ggtgccgcca gcagtcggtg ccgacatcat gcgcctcttc ccggagaaat
     3841 ccgccgctct catgtatcac cttggtgcta atcctgagaa aacacgccag ttgctggcga
     3901 tggacgggca atccgcgctg attgaactca ctcgactgtc agaacgttta actctcaagc
     3961 ctcgagccaa gcctgtttca gaagccccgt tacctgatga acccattcag ggacatgctg
     4021 ttgctgcaaa tatctctgcg attgaaaagc agatggaggc ggcagcaaac aaaggggatg
     4081 tagagacgta ccgcaagctc aaggcgcaac tgaataaagg aattcgataa tggcattaaa
     4141 tgaaggtcaa ctggtcacgt atgctctgga tgaaatcatc ggaaccgtcc agaacctgac
     4201 gccaatggcg tcaaaagtga caaaataccc ccctccggca gaatccatgc agcgttcaag
     4261 caacaccgtg tggatgcctg ttgagcagga agcgccaacc cagactggct gggatttaac
     4321 tggcaacgca accgggattc tggaactctc cgtgaagtgc aacatgggcg atccggataa
     4381 cgatttcttc gagcttcgtg cagatgacct gcgtgatgag cgttcttacc gtcgccgcat
     4441 ccaggcatcc gccaaaaaac tggcgaataa cattgagtca gcgattgcca aacaggcaac
     4501 tgaaatgggc tcgcttgttg ttcacgatac ccgcgcaatt ggtccatcga ctggcctgcc
     4561 tggctgggat tttgtgtctg atgcagagcg actgatgttc tcccgtgagc taaaccgcga
     4621 catgggcatc agttacttcc tgaaccctga cgattaccgc aaagcaggcc gcaacctggt
     4681 agatggtgac atcttcgggc gcgttcctga agaagcatat cgaaacggta ctattcagcg
     4741 tcagattgct ggctttgatg aaattcttcg ctcaccgaaa cttccggcag ttaccaagtc
     4801 aaccgcgact ggtgtaactg tttctggtgc gcagaagttt aagccgcagg catacaccct
     4861 tgataccgat ggtaacaaag agaacgtcga caaccgtgtt gcaacggtga ccgtatcctc
     4921 caccaccgga tttaagcgcg gcgacaaaat cagcttcatt ggtgtgaagt tcctgtctca
     4981 gatggcgaag aatgtgctga ctgatgacgc gactttctca atcacccgtg tgatcgatgg
     5041 tactcacatc gaaatcacgc cgaagccgat tgcgctggat gacgcgtcac tgacaaaaga
     5101 agagaaggct tacgctaacg taaacacctc tcttgctgat accactccgg taaacgttct
     5161 gaacgtggca acaaccaccg ctaacgtgtt ctgggctgat gactcaatcc gcctgctgtc
     5221 tcagccgatc ccggtaaccc atgaactgtt tgctggcatg aaaacgtctt ccttcagcat
     5281 tccaggtatt ggtgttaacg gcatcttcgc aacgcagggt gatatcaaca ctctgtctgg
     5341 taagtgccgt attgctgtgt ggtattcagc atgtgctgta cgaccagagg caattggtgt
     5401 tggtctgcct aaccagaccg cgtgataacc agagggagct tcggctcccg tttttatctg
     5461 gagacaagca tgacacacat gatctttcgt catggcgaca tgaagaagtg gaaaggcgtt
     5521 ggctacgact ttgaaatcgt gaaagccgaa gagcttcagg aatatctgga tgctggctgg
     5581 ttttcacatc ctgatgacct tttgaaggat gttgcagagc cagagccaga agaaaaacag
     5641 cgtaaaaagc ctggtcgaaa acctaaggcg gcagcagatg aacctgacaa cgaagggtga
     5701 tttagttctt gcggcattac gtaagctcgg tgttgcatca aatgccacgt taaccgatgt
     5761 cgaaccgcag tctatggaag acggcgtcaa cgaccttgaa atgatgatgg ctgaatggct
     5821 tggcggtgat gcgtcacctg gtatcaacgt tggctacatt tttgctgatg cagatgttgc
     5881 tccggatccg ggcgatgagc acggtttatc aaataacgct atcaatgccg tcattttcaa
     5941 ccttgcctgc cgaattgctc cagattatgc gctggaagcg tctgcaaaac ttataaccac
     6001 tgccagatac gggaaagagc gactcgtcaa actgtctgca atggacaggg caaaagcagc
     6061 taaatgtaag tccggttatc caaaccgtat gcctgttggt agtggtaacc agttggcgaa
     6121 gtggaatggt tggaattact tccaccgaaa ggaaccttgc gataacggga gcgaataatg
     6181 cctattcagc aacttccgct tatgaaaggt gtcggcaaag acttccgaaa cgccgactat
     6241 atcgactatc tgccagtgaa tatgctggct acacccaaag aaatcctgaa cagcagcgga
     6301 tatcttcgct cattcccggg cattgccaaa cgttctgacg tgaacggtgt atctcgcggc
     6361 gtcgagtaca acatggcgca gaatgctgtt tatcgcgtgt gtggcggcaa gctgtacaaa
     6421 ggagaaagtg aagtcggtga tgttgccgga agtggtcgtg tatcaatggc gcatggtcgg
     6481 acatcacagg cggtaggcgt taatggtcaa ctggtcgagt atcgctatga tggcacggtc
     6541 aaaaccgtct caaactggcc tacagacagt ggattcactc agtatgagtt aggttcagtt
     6601 cgcgacatta cgcgcttacg tgggcgttat gcgtggtcaa aagacggaac tgattcatgg
     6661 tttatcactg accttgaaga cgaatctcat cctgaccgtt acagcgcaca atatcgtgcc
     6721 gagtctcagc ctgacggcat catcggtatc gggacatggc gagacttcat cgtctgcttt
     6781 ggttcatcga ctattgaata tttctccctg actggcgcaa ccaccgttgg tgccgctttg
     6841 tatgttgcac aaccatcgct gatggtgcag aaaggcattg ccggaactta ctgcaaaacg
     6901 ccattcgctg attcgtatgc gtttatcagc aatccggcaa cgggtgcgcc gtctgtgtat
     6961 atcatcgact ccggtcaggt gtcaccaatt gccagcgcga gcattgagaa aatcctccgc
     7021 tcctacactg ctgatgaact ggctgatggt gtgatggaat cgctgcgatt tgatgctcat
     7081 gagttgctga ttatccacct tccgcgtcac gtcctcgtgt acgacgcatc ttcaatcgcc
     7141 aatggcccac aatggtgtgt gctgaaaaca ggtctgtatg acgatgtgta ccgcgctatc
     7201 gacttcgttt acgaaggcaa tcagataacg tgcggcgata agctggaatc tgttaccggg
     7261 aaactgcaat tcgatatcag cagccagtac gacaagcaac aggaacacct gctgtttact
     7321 ccactcttca aagcggataa cgccagagtg ttcgaccttg aggttgaatc gtcaactggc
     7381 gttgcgcagt acgccgaccg cctttttctc tctgcaacca ctgactgcat caattacggg
     7441 cgtgagcaga tgattgagca gaatgaaccg ttcgtttacg acaagcgtgt tttgtggaag
     7501 cgagtagggc gcatcaggaa aaatgtcggc ttcaaattgc gcgttatcac gaagtcacct
     7561 gtcactctgt ctggcgctca gataaggatt gagtaatggc tgattcgaat ctcaatgtgc
     7621 cggtaatcat ccaagctacg cggctcgata catcagtcct tccacgcaat atcttctcgc
     7681 agtcgtatct gctttacgtt atcgcacagg gcactgatgt tggtaacgtg gctaacaaag
     7741 ccaacgaggc cggacagggc gcttatgatg cacaggtcag gaacgatgag caggatgtga
     7801 ttctcgctga ccatgagcag cgaatttctg ctgcggaagc aacgcttgtt aatcatgagg
     7861 agcgaatcag acaggcagaa tcaactcttc aggaccatga aacacgaata gctcagaatg
     7921 aaagcgatat ttcgtcgctt gacacaagag ttcagtcgct ggagtcgcag gtttcagacc
     7981 atgaaacgcg catcgatgct ctggagtatg caaccacacg caagaagtca gaggttgttt
     8041 actctggtgt atctgtaacc atcccgacag cgccgaccaa ccttgttagc ctgctgaaaa
     8101 cgctcacacc gtcatccggc tcgttggcac cattcttcga caccgttaac aacaagatgg
     8161 ttgtgttcaa cgagaacaaa accctgctct tcaagttgtc gattgtcggc acgtggccca
     8221 gcggaaccgc caacaggtca atgcagctaa ccttttccgg ctctgttcct gacacactgg
     8281 tcagcagtcg taatgcggcg acaacaaccg acaacatcct gttagctacg ttcttcagcg
     8341 tggataaaga cggctttctt gccacaaatg gcagtacgtt aaccattcag tcgaatggtg
     8401 cggcgtttac tgccacaacc atcaagataa tcgcggagca gtgatgatta cattcaaacc
     8461 aacgcgaaac atcgacctga tagaagcagt cggaaatcac cctgacatta tcgccgggag
     8521 caacaacggt gatggatacg actacaaacc tgaatgccgt tactttgagg tgaacgtgca
     8581 cggtcagttt ggcggcattg tttactatca ggagattcag ccgctgacat tagattgcca
     8641 cgccatgtac ctgccagaga ttcgcggctt cagcaaggaa attgggctgg cgttctggcg
     8701 atacattctg actaacacca ccgttcagtg cgtaacatcg ttcgctgcac gcaaattccg
     8761 acacggtcag atgtactgcg caatgattgg ccttaagcgt gtaggaacca tcaagaaata
     8821 cttcaaaggc gtggatgacg tgacttttta cagcgccaca cgcgaagaac taatcgactt
     8881 cctgaatcac gggagatagc catgttatat gcatttaagc tgggcagaaa actgcgcggc
     8941 gaggaacctt attgccctga aaaaggcggg aaaggtggca gttctgataa aagcgcaaag
     9001 tatgcagcag aagcccagaa gtatgctgca gacctgcaaa atcagcagtg gcagacgatc
     9061 atgaaaaacc ttgctccgtt cacgcctctt gcggagcagt atgttaacca gcttcagaac
     9121 ctttccagtt tagaaggtca ggggcaggca cttaatcagt attacaactc taagcagtat
     9181 aaagaccttg cagggcaggc gcgttaccag agtcttgctg ctgcggaggc gacgggtgga
     9241 cttggttcga cagccacaag caatcaactg gctacgatcg cgccgacact cggtcagtct
     9301 tggttatcaa atcagatgag caattacaac aatctggcaa acgttgggct tggtgcgctg
     9361 caaggtcagg caaacgccgg gcagacgtac gccaacaaca tgagcagcat tgcacagcaa
     9421 agcgcagctc ttgccgctgc taatgccaat aaaccatcaa gtcttcagac tgcaattagc
     9481 ggtggcacgt ctggtgcgat tgccggtgca ggtcttgcca gccttttggg aacatcaacg
     9541 ccttggggcg ctggtattgg tgctggtatc ggattgcttg gctcgttgtt ttaaggggga
     9601 atcatggcta cttggcaagg aacaaacggc ggattgttgg ctggtatcgg cggcgtcaac
     9661 tcaaacgctc cgagcgtaaa tgacatcggc aatacgcttc agcttatcag gcagaacaat
     9721 gatattgagc gttcaggcgc taacaatgtt gggctgactg ctttgcaagg tctttcaggt
     9781 attgcgggtg tttttcagca ggaaaagcag gctcagcggc agaaagaatt tcagcaggcg
     9841 tacgctaatg cttatgcgtc tggtgatcgc ggtgctttgc gtcagttggc tactcaatat
     9901 ccagaccaga ttgaatccgt tcgtaaaggc atgggattca ttgatgaaga gcagcgtaat
     9961 tctatcggca ccttagcggc tggcgcacgc cttgcgtcat cgtctccaga agcaatgcaa
    10021 tcatggctgc aaaacaacgc cggtgagtta gctcgtgttg gcgttaatcc tcatgacgtc
    10081 gctcagatgt accaacagaa cccgcggcag ttcggcgaat ttgtcgatca cctggggatg
    10141 aacagtctcg ggcccgaaaa atactttgac ctacaggata aaatgcaggg tcgccaggtt
    10201 accatgcgcg gtcaggatct ggattcgcaa accgccgctc ggaatcaggc aatcacaatg
    10261 cgcggacaag atatccaggc gaatttaggt cagcagcgca ttaatctgga cgcagaaaca
    10321 aaccgcatta acaacgaaaa taagcgcctt gaccggatgc tatcagcaga aactaacgac
    10381 ctgaagcgcc aggaaataca gagccgcata gcagccaaca accagcagtt gcagcagaag
    10441 cagcaagcgc taaatgatgg ctacaaagac ggcatcaaca ccctcacaac cagcatgttc
    10501 actctgaacg atatcgttag ttctccttca cttaagagca ttacaggctt acgtggagta
    10561 atccccaacg ttccaggctc acaggctgca gacactcagg cacgacttga tacctttaaa
    10621 tcacaagcat acctgacagc ggttcaggcc atgcgaggca tgggcgcact ttctgatgcc
    10681 gagggcaaaa agctcgacca ggctgttggt tcgctgcaga actcgcagag cgaggagtcc
    10741 tttcgtcgca acgctggcgt catcctgaac acgctcaacc agaagcgtaa tgaggcggtt
    10801 ggtaagtacg ttcagcaaaa cggtatcaag cgagtggaag cgcctcaggc ttctatagat
    10861 tacctgaagc agcaccccga gctgtcaatc gacttcatta atcgctacgg atatcttcca
    10921 tctttggggc agtaaatggc taattaccgt gatttgttag agcaggctgg cgcacgttac
    10981 ggtgtgccag aagggttgat gactgcactg ggtgccaagg agtcttctta caaccctgcc
    11041 gcagtaagct ccgccggggc tgtaggattg actcaggtca tgcctgggac atggcgtgat
    11101 atgggttata ccgatgagca aatgcaaaac cccgaatatc aggctgacgc tggcgcgcgc
    11161 tatctggcaa agatgtacca gcagtttggt aactggcgtg actctcttca ggcttatcac
    11221 gacggtcccg gcaacgttat gaaggcaaag cgtggtgaat atacgccagg acctgaaggc
    11281 cgcggttacg ttgatgatcg ctttgctcaa tgggctggtg acccggtgac agactcaaca
    11341 gtcgaacagc gcgccacctc tgcaaaggta catcctcagc aagaccctaa caacccgttt
    11401 gcacaactgg aagtacagtc atccgaacaa gtatcggcat caggtgtgca gtcagaccca
    11461 aataatccat ttgctcagat tgagcagcag gcagccagtc agcagccacc tcaacccgta
    11521 agttctgtcg caccgaaacc tgttcagcaa caaacagtta atcaggccaa taatgcacca
    11581 acacgggaag aaccatcatt gatgcaacaa gctggcgatt ggctcactgg tggtcaaagt
    11641 gcagggcaaa ttgcagaaca ggctggtcgt ggtctggtaa acataccatt tgacgtattg
    11701 cagggtggcg caagtctgat taatgcaatc agtcaggggc ttggggggcc aaaagtattg
    11761 gatgatgttt atcgtccagt agacagaccg acagacccct acgcgcaagc tggagagtca
    11821 ataggcggtt atcttgttcc tggagcaggt gtagctggaa acatggtcat tggttctctc
    11881 gctgacgcgg cgaatcaacg gggtgatttt gccgaaaatg ccgctattaa tgccggactt
    11941 agcattgcta cgcatggcct gataaatggc gttacccgtg gtgttcgtgg tgcatcaaat
    12001 ataattagtg gcaataaaac atctgcacag agagcgacca ctgcgccaac agaaacatca
    12061 ccattctccg gtgatgccgc tgcagcaaca aatcctgcgg ttcatgccgc agaggcaaga
    12121 gtagcacaag gtgtaccaat gacgcctgcg acgaagaacc cagaggaagt cgttcgcaca
    12181 gtagcagcac aaaaaaggcc aaatctcgct tcatcgcttg atgaactaga tatcaatcct
    12241 caggctgaag ttctggagtc tgctgaaagg cttaatgttg attcattact cccttcacac
    12301 ttttccggga acgagcaata caaggcagtt gagcaagcaa tcaagtcccg tgcgggttct
    12361 gctctacagg tgcaggaaaa tgaagcaatc aggcagctag cacagggcgc gggtgagata
    12421 attgatcgcg tttccggtgc aaaagatgct cttggtatga gcgacaagtt tattgatacg
    12481 gtcaatggaa gaatgtctgc gctgatgaaa cgaagcgacc agctttatcg caatgttgaa
    12541 aaggcgatgc ctgcaggtgc aaaaattgat gcgccatcaa caaggtcaat gctcaaacag
    12601 gtggcagaag atcttggcgg gatgaaaaac cttgacccta ttgaaaaga
//
LOCUS       DAJTSA010000107        12634 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000107 DAJTSA010000000
VERSION     DAJTSA010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.107"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            758..841
                     /locus_tag="OO369_003310"
     CDS             758..841
                     /locus_tag="OO369_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR5983469.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(895..2247)
                     /gene="gorA"
                     /locus_tag="OO369_003311"
     CDS             complement(895..2247)
                     /gene="gorA"
                     /locus_tag="OO369_003311"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR5983470.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="OO369_003312"
     CDS             complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="OO369_003312"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR5983471.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATDIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3364..5406
                     /gene="prlC"
                     /locus_tag="OO369_003313"
     CDS             3364..5406
                     /gene="prlC"
                     /locus_tag="OO369_003313"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR5983472.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5414..6166
                     /gene="rsmJ"
                     /locus_tag="OO369_003314"
     CDS             5414..6166
                     /gene="rsmJ"
                     /locus_tag="OO369_003314"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR5983473.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="OO369_003315"
                     /pseudo
     CDS             complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="OO369_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="OO369_003316"
     CDS             complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="OO369_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR5983474.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8830..9165
                     /gene="uspB"
                     /locus_tag="OO369_003317"
     CDS             8830..9165
                     /gene="uspB"
                     /locus_tag="OO369_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR5983475.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="OO369_003318"
     CDS             complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="OO369_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR5983476.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10967..12166
                     /locus_tag="OO369_003319"
     CDS             10967..12166
                     /locus_tag="OO369_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR5983477.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3109 a         3169 c         3356 g         3000 t
ORIGIN      
        1 taacagaaat gacatattgc gctcctgatt gttgcaggta gtgtctctct tcgaagcgga
       61 taagtcaaaa acatatatga cttaacgaat gtgtaagtgc agaggaaggt aataggtgtg
      121 aattttgagt tggctattca tttgaaaggc ggtttgaatc aggacgtaaa aaaaccgact
      181 ttgcgtcggt tttttactct tcagcctgag ttggtggctc tggctggagt gagaatgccc
      241 acatctaacg gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt
      301 cagctggaga agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat
      361 cgggtcgctg ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc
      421 ggttttttta ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc
      481 taacgcatca atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc
      541 taattacact gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat
      601 aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg
      661 gagtgagatt gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa
      721 gcggatgcat tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt
      781 tcttggggcg ttaattgcgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg
      841 atagcggaaa cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg
      901 catggtcacg aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt
      961 tttggttgcg cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa
     1021 gccaatgccg tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg
     1081 gcacggctgg cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac
     1141 tttcacctga tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc
     1201 aatcggcgga tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg
     1261 cttgttatta aacaggcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag
     1321 ttccactgca cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata
     1381 tttatcgacg acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat
     1441 gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg
     1501 accatcttcc agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat
     1561 ggcgttggta tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat
     1621 catcgggtcg aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc
     1681 gaggccgtta atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac
     1741 gcgctctggc aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc
     1801 tggaatatcc gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat
     1861 ggtttcgccg ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac
     1921 atcaacgtta tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc
     1981 ggtacggctg gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata
     2041 atcagggccg tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt
     2101 cggcacgcag ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca
     2161 tttctggccg tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat
     2221 ggcgatgtaa tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt
     2281 gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt
     2341 acggtggcgt gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta
     2401 ttcatctgct gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg
     2461 ccacggcgat cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgtcg
     2521 gtcgcttcca ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac
     2581 aatgcgtaag tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc
     2641 tgatagtcgg ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc
     2701 ggcggcagtt tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg
     2761 tctttctgaa atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc
     2821 aggctgtctt gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc
     2881 aactggccgc tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc
     2941 aaatcgtcct gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca
     3001 tgttcgctac ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt
     3061 ttatctttct ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg
     3121 gcgtggttgc cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta
     3181 agtaaaaatt ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag
     3241 aggccgccgc cgcgaaagcg taacgtttct cattgaaatt cactacactt aaccccatgc
     3301 tacacacatt atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg
     3361 ctaatgacga atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg
     3421 gaacatgtcg ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc
     3481 gtagtagcgc aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg
     3541 gacgatgttt tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc
     3601 ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg
     3661 gtagggcaac atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac
     3721 gccacgctga acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc
     3781 tctggcatag gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt
     3841 tctgaactgg gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa
     3901 ctcgttaccg acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa
     3961 gcccaggccg aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac
     4021 ctgccggtaa tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac
     4081 agcacccgcg cctccgatca aggcccgaat gccggtaagt gggataacag caaggtgatg
     4141 gaagagatcc tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc
     4201 tttaaatccc ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc
     4261 gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc
     4321 gctaaagccg aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa
     4381 aaacagaaac agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa
     4441 aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa
     4501 gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa
     4561 aataacgaac tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcggc
     4621 ggggcgtgga tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa
     4681 ccggtcgcgt atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc
     4741 actcacgacg aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg
     4801 acccgcatcg aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa
     4861 ctgccgagtc agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct
     4921 ggtcactatg aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg
     4981 aagaactacc aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc
     5041 cgccttcatg ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa
     5101 atcaagaaac tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc
     5161 agccatattt tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta
     5221 ctggcggcag atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg
     5281 cagtcgttcc tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc
     5341 aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag
     5401 ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt
     5461 atctgttctg gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt
     5521 gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt
     5581 tgttgatttt gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga
     5641 ggcggtggcg aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac
     5701 cgcagggctg gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct
     5761 ggagcgtaat ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga
     5821 tgcggaaatc ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac
     5881 ggcgctaacc gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca
     5941 taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc
     6001 ggatcttgat gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt
     6061 ggtaaagcgc ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt
     6121 gactaaaggg catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt
     6181 agcgataacg ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca
     6241 tgcgtttcag ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac
     6301 caatcttacc aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt
     6361 ccggcaccgc ggtaaatgtt gccacatagc cgcccagcaa gaacgcggca gcctgcgtca
     6421 ggaaccacat cccgagaata aagcccatca aatgctgcgg caccagggca gcaatcatcg
     6481 ccaggccaag ggcgctaata aacaattcac ccaggctcta gaataaatac accagcacga
     6541 taaaccatgg cgatgtcagc ccctggtcat ccgcaaacca cattcccgca gcagcagccg
     6601 tcaaaaagcc cagtgagcac ataaacatgc tgagagtaaa tttcatcggc atcgagaggt
     6661 ctttgccttt gttacccaga tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca
     6721 cccagaacgg gttaagcgcc tggaagctga ccgggttgat ggaaaaaccg agaatttcat
     6781 gatgcacgtt gttgatggca aagaagttca gcgatgttgg catctgggcg tagagaatgt
     6841 aaaacaccac cgcttcgagc atcaggacaa aggcgacaaa cattttattg cgcccggttt
     6901 tatccagctt gaatgcctga cgaaagaaga tgatagtgac gacgatggag agaacaatca
     6961 gcaccagatt ggcgacttct acgttgtgca tcagccatgc acatacgaag atcatcacca
     7021 cgctgccaag taatacgtac aacagtttgc tgaagctcat cggcttgaag tcgggttcag
     7081 aaccaatgtc tttcaccatt ccacgacagg cgatgtaaac cagtaatgcg ataattaacc
     7141 ccgccccgca caggttgtag gtgactgaat aaccgaatct atcagcgatc acaggggcca
     7201 gcgataacgc tatcaacgag ccgatattga tcgacatata gaacagggtg aatgcgccat
     7261 caagccgcgg atctttcggc ggatagcact tcgaaagcaa gctggctggg ttagctttaa
     7321 acaggccgtt accgacagcg atagtcccca gggcgatgaa aatcaggtca ggcttaagta
     7381 gcgacatgcc ggtcatgaag tagccaatcg ccagcacaag tgctccaaga acaatggtgc
     7441 gtttggtccc caacaggtgg tcgccgacat agccgccaat ggaaatgagg ccatagacca
     7501 gtgcagcaaa agcaccaaaa gtgacaaaag cctgctcttg cgagaatcca agctgtttaa
     7561 cgaagaaaac cgccagtacg ccctgcacgc cgtagtagcc gaatcgctcc cataactcga
     7621 caaaaaagat catgaaaaat gggcgaggtt gctgcagcat ccccatgggt gttgttgtat
     7681 taatatttat taaccttcca ataccatcct gaaaagtatg aacgcgtaag ggataacgcg
     7741 cgaaaaagtt tacatctgtc agagattctt aatattgatg cggtgaatag attaatacac
     7801 tattacaaca gaaaataacc agctgatcac actaatgatg gaaaatatga tggtgttaaa
     7861 ttttgtattg gcaggagatt ttgattgcgt acgcagatcg taggccagat aaggggttta
     7921 cgctgcatca ggtaacaagg cgcgccaaaa agcaaaacgc ccgcatatgc gggcgttcag
     7981 gaacacgtcg tggagggatg attattcttc ttcgtcgcgt agcggaacaa tcagcatatc
     8041 aacgtgaacg gtgttgatca gctgacgtgc ggaagacatc agtttgctcc agaagtcctg
     8101 gtggtgacca caaaccacca gatccatatc gtatttcttg attgcatcga ccagaacctg
     8161 gcctaggtcg ccgctgccgc tcagggtttc agtgattgga tagcctgcat tagtggaaag
     8221 ctcggtcagc gcatgatgtg tctcttcaga gatgcgtttc tgcatatcac ccagattcac
     8281 atcaataagc ccggtgtata ggtcagagta gtttacatct acgtggatca gagaaacttt
     8341 cgcattgtag gggcgagcca tagagactgc tttctctacc agaactttgc tttccgggga
     8401 gagatcgact gcgatgagaa tgtgtttata agccatagtg ttactccttc cataaagttg
     8461 tcgatgactg gccagctagc gtttcttgtg ccgttacgtc acccgcgtcc tgcgaactac
     8521 gtgcgcgggg ctaacccagc cgtaaagatt cagtgttaag accatcctta ccttatagcg
     8581 acccggatga tccgtcaatc cgccttgctt acccgttaga taaacaaatt ttcgacatga
     8641 atattatatt gatagtggtt aaccttctgg aaaaaaaaca acctgatctc ctacactatc
     8701 tatagagccg ctcggatgtt ggtcaccaca aaagcggttt ctccaggacc tggtgttaac
     8761 tgattggcag cgtatcggga gcggtagccc cggggtggaa ctccgcgggc aggtcgccgg
     8821 ggaggaggta tgataagcac cgtcgcatta ttttgggctt tatgtgtcgt ttgcattgtt
     8881 aacatggcgc gctatttctc atcactacgc gcgttgttag tggtactgcg taactgcgat
     8941 ccattgctct atcaatatgt tgatggaggg ggctttttta cctcacatgg ccaacccaac
     9001 aaacaggtgc gtctcgtttg gtatatctat gcccaacgtt atcgcgatca tcacgatgat
     9061 gagtttattc gccgctgtga gcgggtgcgt cggcagttta ttctgactag cgcattgtgt
     9121 ggtctggtgg tggtcagcct gattgcattg atgatttggc attaagcact tcagcaataa
     9181 taacgtataa aaaaagcggg ccagtaaact gacccgctcg ttttggtgcg tacgattaca
     9241 ggaactgcaa ggagaaccag tacagcccgc cggaaagtag taccgcagcc ggaagggtaa
     9301 acacccaggc catcagaata ctggttacgg ttttacgctg taagccgccg ccatctacca
     9361 ccatcgtccc cgcgacagaa gaggagagta cgtgagtggt ggaaaccggc atcccggtat
     9421 aactcgccag gccgattgac actgccgccg tcatctgggc agacatcccc tgagcgtagg
     9481 tcatgccttt cttaccgatt ttctcaccga tagtcgttgc cacacgacgc cagccaatca
     9541 tcgtaccgat acctaacgcc agcgcgaccg ccatgatgat ccacaccggc gcatactcga
     9601 tggtgctaag catgtcggac ttcagtttct tcaacaggcg ctgatcgtca gcactcacac
     9661 caggcatctt caccactttg tcgatagtgt cagaaacgca cagcataatg cggcgcatct
     9721 ggctacgttg atcaagcgac agcttgtcgt agctttccac atcggtggtc aacatacctt
     9781 tcaggcggtt gagcgcgtta atggtattcg acggatggca gtggaactcc gcaggttgcg
     9841 ttgcgccagc ttccggagcc ggtactaact gatcagcacc ggtagcctgt ttgagcagcg
     9901 caggatgctg ctcaaagtaa gcttcgacgt tgttgatggc atcacgggta cgggtgattt
     9961 cgtagccagt ggcattcatg ttcaccacga agcctgctgg cgcgacgcca atcaatacca
    10021 acataaccag accaatgcct ttctgaccat cgttcgcgcc gtgcgaaaac gccacgccga
    10081 tagcggaaag gatcagcgcg atacgcgtcc agaacggcgg ctttttcttg ccgtctttct
    10141 tttcacgctc cgctggggtc aggtggatac gggcgcgttt cttggtgccg ctccagtagc
    10201 gacgcagcaa gaaaatcaga ccgccagcaa acaccaggcc gacaataggg gaaacgatca
    10261 gagaaccgaa aatacttaat actttcggga tattgagtgc atccaccact gacgtcccgg
    10321 tcatcaacgc attggttaaa ccaatcccga tgatcgcgcc aatcagcgta tgagagctgg
    10381 atgcaggtaa accaaagtac caggtaccca ggttccagat aatcgccgcc agcaacatag
    10441 agaacaccat ggcaaggcca tgagacgatc ccatattaag cagcagatcc gtcggcagca
    10501 tatgcacaat ggcataggca acactcagac cacccagcaa aacacccaaa aagttgaaca
    10561 ccgccgccat aaccacggcg agctgagaac gcatcgcgcg ggtatagata acggttgcca
    10621 cggcgttggc tgtgtcatgg aaaccattga tggcttcgta gaacagcaca aaagccagtg
    10681 caagcaataa taacagcccg gtatgcaaat ccaggccagc aaacaaatgt agcataggac
    10741 gttacgccat tttgaggaca tgaacgcggc gcattatcag tgactttcgc ggcgcgggca
    10801 aagtgaaata tagacttttt ttgatttgcc tcctgtatgg atttcactca aaaaataatt
    10861 atcttatata attcaggcaa atacttcctt ttagtaatat tgatgctggt gcgaccactg
    10921 aggaatcttt acaattcacg cccgtttttt ctaagaggag cgcaacgtgg aaaggtttga
    10981 tgccattatt ataggcgctg gtgcggcggg tatgttctgt tctgcgctgg caggtcaggt
    11041 aggacgccgg gttctgctga tcgataatgg taaaatacta gggcgcaaaa tccttatgtc
    11101 tggcggtggg cgctgcaact ttaccaacct ttatgtcgaa ccaggcgctt atctgagcca
    11161 gaatccgcat ttttgtaagt ctgcactcgc acgttttacc cagtgggatt tcattgatct
    11221 ggtcaataaa cacggtatcg cctggcacga gaaaacgtta gggcaactct tctgcgatga
    11281 ctccgcgcag cagattgtcg acatgctggt ggatgagtgc gagaagggca atgtaacctt
    11341 cagattgcgt agcgaagtgc tgagtgtggc gaaggatgaa acaggcttca cgcttgatct
    11401 gaacggcatg actgtcggtt gcgaaaagct ggtcatcgcg accggtgggc tgtcaatgcc
    11461 ggggctgggc gcatcgccgt ttggttataa gattgccgaa caatttggcc tcaacgtgct
    11521 gccgacccgc gcgggtctgg tgccattcac tctgcataaa ccgttgctcg aagagttaca
    11581 ggtgctggcg ggcgtggcgg tgccttccgt gattaccgct gaaaacggca ccgttttccg
    11641 tgagaactta ctcttcaccc accgcggctt atctggaccg gcggtgttgc agatttcaag
    11701 ctactggcaa ccgggtgaat ttgtcagtat caatctgcta ccggatgtgg acctcgaaac
    11761 cttcctgaat gagcagcgta acgcacatcc gaatcaaagc ctgaaaaaca cactggcggt
    11821 tcatctaccg aagcggttgg ttgaacgctt acagcaactc gggcaaatcc cggatgtttc
    11881 gctaaaacag ctcaacgtgt gtgaccaaca ggcactgatt agcacattga ccgactggcg
    11941 cgtacaaccc aacggcactg aaggctatcg cactgccgaa gtgacgctcg gcggcgtgga
    12001 caccaacgaa ctttcttcac ggacgatgga agcgcgcaaa gtgcctgggc tgtacttcat
    12061 cggcgaagtg atggacgtta ccggctggct ggggggctat aacttccagt gggcgtggtc
    12121 gagtgcatgg gcttgtgcgc aggatttggc gtcaattgtg aaataaccat gacgttttcc
    12181 cgtcagccgt ttgtatatgt cgttattaag tcattttggt acggttctgc atccgacatt
    12241 ctggtgttaa ctcgctgttt tgttagcgaa ttaacgctat tgccgttgtg gttattttat
    12301 agtgtattta ttctcagaga atatatatta ctgaaataaa aaacacaaag aataaccaaa
    12361 ggaaattatt tgaaacgtaa aaggtatata tttttaatct gtaaaattta aaatcacctc
    12421 ctctggattg aatcaacttg ctctatttaa gacgattgtt tttacatttt caccacctgt
    12481 atcaggtggt gtttttttat tggcggcaat gcctttatct tatttttatt acgttgtaaa
    12541 tatggctatt cctaaatgaa atttgatttg ctcagtttat ttgtgatgtc aggggagtgc
    12601 ttgccatgag agtcgtgata ttattattca tcat
//
LOCUS       DAJTSA010000108        12571 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000108 DAJTSA010000000
VERSION     DAJTSA010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12571
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.108"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(82..801)
                     /locus_tag="OO369_003320"
                     /pseudo
     CDS             complement(82..801)
                     /locus_tag="OO369_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(817..1149)
                     /gene="cusF"
                     /locus_tag="OO369_003321"
     CDS             complement(817..1149)
                     /gene="cusF"
                     /locus_tag="OO369_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCR5983478.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1173..2555)
                     /gene="cusC"
                     /locus_tag="OO369_003322"
     CDS             complement(1173..2555)
                     /gene="cusC"
                     /locus_tag="OO369_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR5983479.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            2712..3395
                     /gene="cusR"
                     /locus_tag="OO369_003323"
     CDS             2712..3395
                     /gene="cusR"
                     /locus_tag="OO369_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR5983480.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3385..4827
                     /gene="cusS"
                     /locus_tag="OO369_003324"
     CDS             3385..4827
                     /gene="cusS"
                     /locus_tag="OO369_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR5983481.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            4977..7214
                     /gene="nrfB"
                     /locus_tag="OO369_003325"
     CDS             4977..7214
                     /gene="nrfB"
                     /locus_tag="OO369_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCR5983482.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7201..10169
                     /gene="nfrA"
                     /locus_tag="OO369_003326"
                     /pseudo
     CDS             7201..10169
                     /gene="nfrA"
                     /locus_tag="OO369_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10170..11060
                     /locus_tag="OO369_003327"
     CDS             10170..11060
                     /locus_tag="OO369_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR5983483.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11243..12004
                     /gene="envY"
                     /locus_tag="OO369_003328"
     CDS             11243..12004
                     /gene="envY"
                     /locus_tag="OO369_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR5983484.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12050..12126)
                     /locus_tag="OO369_003329"
     tRNA            complement(12050..12126)
                     /locus_tag="OO369_003329"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12090..12092),aa:Arg,seq:tct)
     gene            12369..>12571
                     /locus_tag="OO369_003330"
     CDS             12369..>12571
                     /locus_tag="OO369_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR5983485.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3057 a         3276 c         3201 g         3037 t
ORIGIN      
        1 ggttgttttt cgagttataa cagacagtta attaactaca gtggacaatg ctagacaatg
       61 acagacacag ataagcaacc taccttcctc tttcgcgacc acgttatctt tggcgatatt
      121 cattcccgcg cgcagatcaa acgcggtgat cacgccatca atgggcgctt tgagcgtaaa
      181 gcgagtctgg attttttgcg tggcgatcag gtggcgaata tccgcctccg gcattcccgc
      241 cagtcgcagc cgctcaagaa tgccttcagt ctgggtcgcc gtaccgccgg tttcgcgcaa
      301 cagtaaatac tcactctgcg cttccaccca gtcgggaatg gtcaggtcga gaagcggtgt
      361 gcccttttgt actttatcgc ccacggtaag cggatacacc ttgtcgataa acccggcagc
      421 gcgagcctgc acaatggcat actgatactc gttgtaactg acattcgtcg ggaaactctg
      481 ggcaaaagtc agcggcccgc gcgtgacggt agccgttttc acccccagat tctgcgtctg
      541 agtcgggtca atgcgcacac cagacgcaga actctcttca tcggcatatt tcggcaccag
      601 atccatatcc ataaacggcg atttacctgg tttatcgaac cgcgtattgg gatacatcgg
      661 gtcgtaccag aataagattt tacgttctgc ggtcggcatt ttttctgcgt gcggttccgc
      721 ctggtcaaac caggtaaaac ctgccgctga aataataccg cccgcgatca tgctgccgat
      781 aataagcgcg atttttttca tctcattaaa cctgggttac tggctgactt taatatcctg
      841 taataaagaa aggttgccct gctggacaaa attaaacgcc actttgtcgc cggttttaat
      901 ttcactcatt ttcgtctgcg gggtgatggt aaagcgcatg gtcatctccg gccagttgac
      961 ggcagcaatc ggatcgtgat ggatggtgat ttttttgctt tccagatcaa caccttttac
     1021 cacaccggtg gcactaatga cctgtggttg tgcttcgctc atggtttcat gatgatgttc
     1081 gttagcctgg gcattaaagc caataacggt aaacagactg aacattgcga cttgcagtgc
     1141 ttttttcatt tattctctcc tggagttaaa aattattgct gccaaccgcc ccctaaggcg
     1201 gtatacaaat aaatttcgtt aacctgacgg gcataattca ggtcaagtaa agtttgtcgg
     1261 gttgcaaata aagaacgctc ggcatccagc acttccagat aacttaccgc gccgtgctga
     1321 tataatgccc gcgcccgttg cagggtaatt tgcagcgacg ccagataacg ctgctgggcg
     1381 ctgatttgat cgtccaggct ttgacgtaat gccagcgcat ctgccacctc tttaaaggcg
     1441 ttctggattt tctgttcata attcaccacc gactgctgct ggcgaatttc ggcgatatcc
     1501 agattggcct ggttgcgtcc ggcattaaaa atggggatct caattttagg aataaaattc
     1561 cacatcccgc tgctagcgtt aaataatgac gacagatcgc tgctggcggt tgatattccg
     1621 ctggtcaggc tgatagacgg gaaaaatgcc gcacgcgcag caccaatatt agcattagcc
     1681 gccattaacg cgtgttcagc ttccataata tccgggcgct gcaataagat ttgcgacgac
     1741 aagcccgccg gtaatttaac gctttgcagg ctgccgctgt ttactgtctg cgcttgcggt
     1801 agcttgccgt agcttcccaa taacagttgc aatgcattat tcgcctgcgc cagttccccc
     1861 tgacgtttag cgatgtcact gcgggtactt tctatcaccc cgcgagcctg ttccagcgcc
     1921 agaacattgc tgctaccggt caacagttgt ttttcgacaa acgcatatga ctgctgataa
     1981 ttacgcagcg tttcttcggc tatttgcaat tgcgcatacg ccagctgctg attgaaatag
     2041 ctttgcgcga cattagaaac cagcaaaata tgcaccgcgc gctgggcttc ctcagtggct
     2101 aaataatttt ggcgctcggc ttcgctcatg ttctttaagc gaccgaaaaa atcgagatca
     2161 aagctggcgt taaggccagt cgagaactcc cgcgtcgtgg ctgaatcgcc tttaagattg
     2221 ccgctccagc tgccgctgcc ctcgccattg agctgtgggt agcggtcggc atcggtcaga
     2281 cgatattgcg cccgtgcctc ctgcactttc agcgtcgcca tgcgcaaatc ccggttattc
     2341 accagcgcct cgctaatcag cgtcttcacc tgattatcaa caaaaaaggt gcgccagccc
     2401 acgttctggt agttatccgc tgcgttaacc aggccgttct ggctgagtga gaactgctgc
     2461 ggtacgggca ttaccggacg ctgataatcc ggtgccagtg aacaaccagt tagcgcaagg
     2521 gccacacaaa atggcagaag tttacaagga gacataggct cataatttct ggtgatttta
     2581 tgtcgccaac tttactcgcc aggctctgat tttccggtga caggaaaatg acaaaattgt
     2641 cattttgcca ataagcgatt gccatctgat cccgctactc tagaattgcc cgggcaacat
     2701 gcggaggaaa tatgaaactg ttgattgtcg aagatgaaaa gaaaaccgga gaatacttga
     2761 ccaaagggtt aaccgaagcc ggttttgtgg tcgatttggc cgacaacggg ctgaatggct
     2821 accatctggc gatgaccggt gattatgacc tgataatcct cgatattatg ctgccagacg
     2881 tgaacggctg ggatatcgtg cgcatgttgc gctccgccaa taaagggatg ccgattctgt
     2941 tgcttaccgc gcttggcacc attgaacatc gcgtcaaggg gctggagttg ggggcagatg
     3001 actacctggt gaaaccgttc gcttttgctg aactgctggc gcgggtgcgc actctcctgc
     3061 ggcgcggggc ggcggtgatt atcgaaagtc agtttcaggt tgccgatctg atggtcgatc
     3121 tcgtcagccg caaagtcacc cgcagcggca cgcgcatcac tctgaccagt aaagaattta
     3181 ctctgctgga gttttttctc cgccatcagg gcgaagtgct gccccgctcg cttatcgcct
     3241 cgcaggtatg ggacatgaat ttcgacagcg acactaacgc cattgatgtg gcggtgaagc
     3301 tgctacgcgg caaaatcgac aacgactttg agccgaagct gattcaaacc gtgcgcggcg
     3361 tgggttacat gcttgaggtg ccggatggtc agtaagccat ttcagcgccc gttttcgctg
     3421 gcaacccgcc tgaccttttt tatcagccag gccaccatcg cggcgttttt cgcctttgca
     3481 tggatcataa tccactcagt aaaagtgcat tttgccgagc aggacattaa tgatttaaaa
     3541 gagattagcg ccacccttga acgggtacta aatcaccctg acgaaacgca agcccgacgc
     3601 ttaatgacgc tggaagatat cgtcagtggt tattccaacg tgttgatttc cctggcagat
     3661 agccacggca aaacggtgta tcactccccc ggtgcgcctg atatccgcga gtttgcacgt
     3721 gatgccatac ccgataaaga cgcgcggggt ggcgaggtgt atctcctttc cggcccgacg
     3781 attatgatgc caggccacgg tcacgggcat atggaacaca gcaactggcg gatgattaac
     3841 ttgccggttg gcccgttggt ggacggcaaa ccgatttata cgctctacat cgcgctgtcg
     3901 atcgattttc atcttcatta cataaatgat ttgatgaata aacttattat gaccgcatcg
     3961 gtaatcagca tcctgatcgt ctttatcgtg ctgttggcgg tacataaagg tcacgcgccg
     4021 atccgcagcg tcagccgtca aatccagaat atcacctcga aagatctcga cgttcgcctt
     4081 gacccgcaga ccgtgccgat tgagctggaa cagctggtac tgtcgttcaa ccatatgatc
     4141 gagcgtattg aggatgtctt tactcgccag tccaatttct cagcggatat cgcccacgaa
     4201 attcgcacac caattactaa tctcattacg caaacggaaa tcgccctcag ccagtcgcgc
     4261 agccagaagg agctggaaga tgtgctctac tctaatctcg aagagctgac gcgaatggcg
     4321 aaaatggtca gcgatatgct gtttctcgct caggccgata acaaccagct aatccccgaa
     4381 aagaaaatgc tcaacctggc ggatgaagtc ggcaaagtgt tcgatttttt cgaggcgcta
     4441 gcggaagatc gcggcgtgga gttgcggttt gttggcgaca aacgtcaggt tgcgggcgat
     4501 ccgttgatgc tgcgtcgggc gctaagcaac ctgctttcta atgcccttcg ttatacgcca
     4561 accagagaga caattgtagt acgctgtcag acggtcgatc accaggtgca agtttccgtc
     4621 gaaaaccccg gtacgcccat tgcgcccgag cacttaccgc gattgtttga ccgtttctat
     4681 cgcgttgacc cctcccgcca gcgaaaaggt gaaggtagcg gtattgggct ggcgatagtg
     4741 aaatcgattg ttgtcgcgca taaaggcacg gttgcggtaa cgtcagatgc gcgggggaca
     4801 aggtttgtta tcacattacc cgcttaatta ttcagcaaac ggcaactttt ataaccagtg
     4861 taaaaataac gtgccgcaat agtcatacat attaatggta aaaagctgtc acaattcata
     4921 aaaaacctta atatacgcca ccctaaacat aaccagcgtt aatgtaaggt ttttgtgtgg
     4981 actggcttct tgatgttttt gctacctggc tctacggctt aaaagtaatc gcgataacgt
     5041 tagcggtcat catgttcatc agcgggctgg acgatttttt tattgatgtc gtctactggg
     5101 tacgccgcat taaacgcaag ttgagtgttt atcgccgcta cccgcgaatg agttaccgcg
     5161 aactgtataa accagatgaa aaaccgttag cgattatggt tccggcgtgg aatgaaacgg
     5221 gcgtcatcgg caatatggcc gagctggcgg cgaccacgct cgactacgaa aactatcata
     5281 tctttgttgg cacctacccc aacgaccccg atactcaacg tgatgttgac gaagtgtgcg
     5341 ctcgctttcc gaacgtgcat aaggtagtct gcgcgcgtcc tggtcccacc agcaaagccg
     5401 actgcctgaa caacgtgctg gacgccatca cccagtttga acgtagcgcc aatttcgctt
     5461 ttgctggttt tattctgcat gacgccgaag atgtgatttc accgatggaa ttgcgtctgt
     5521 tcaactatct ggtcgagcgt aaagatctga ttcagatccc agtgtatccg tttgaacgcg
     5581 aatggacgca cttcaccagc atgacttaca ttgatgagtt ttcagaactg catggcaaag
     5641 atgttccggt gcgtgaagcc ctcgccggac aagtgcccag cgcaggcgtc ggcacctgtt
     5701 tcagccgccg cgccgtgacc gcactgttag ctgacggtaa cggtattgct ttcgacgtgc
     5761 agagtcttac tgaagattac gacattggct tccgcctgaa agaaaaaggt atgacggaaa
     5821 tttttgtccg ttttccggtg gtggacgaag ccaaagaacg cgagcagcgt aaatttttac
     5881 agcacgcacg gacgtcaaac atgatctgcg tgcgcgaata tttccccgat accttttcga
     5941 ctgcggttcg acaaaaatct cgctggatca tcggcattgt tttccagggc tttaaaaccc
     6001 ataaatggac ctccagcctg acgctgaact actttctctg gcgcgaccgc aaaggggcaa
     6061 tcagtaactt tgtcagcttc ctcgcgatgc tggtgatgat ccagcttttg ctgttgctgg
     6121 cgtatgaaag tttgtggccc gatgcctggc atttcctttc tatttttagc ggcagcgcat
     6181 ggttaatgac cctgctgtgg ctaaactttg gcttgatggt taaccgcatc gtgcagcgag
     6241 tgattttcgt cactggctac tacggcctca cgcagggcct gctttccgtc ctgcgtcttt
     6301 tctggggcaa cctgattaac tttatggcta actggcgcgt gctgaaacag gttcttcagc
     6361 acggcgatcc gcgtcgcgtc gcgtgggata aaacaacgca tgacttcccc agcgtcactg
     6421 gcgatacccg ctcgttgcgc ccgttaggtc aaattctgct ggaaaatcag gtcatcactg
     6481 aagaacaact cgatacagca ctgcgtaatc gcgtcgaagg tctacgcctg ggcggttcaa
     6541 tgctgatgca ggggctgatt agcgccgagc agctggcaca ggcgctggca gagcaaaacg
     6601 gcgtggcgtg ggaatccatc gatgcctggc agatcccgtc ctcgctgatt gccgaaatgc
     6661 cagcctccgt agcgctgcat tatgcagtac tgccgctgcg tctggaaaat gatgagttaa
     6721 ttgtcggcag tgaagatggt attgacccgg tttcgctggc ggccctgacg cgtaaagtcg
     6781 gacgcaaagt gcgttacgtc attgttctgc ggggacaaat tgtcacaggg ttacgccact
     6841 ggtatgcacg ccgacgcggt cacgatccgc gggcaatgtt gtacaatgcg gttcagcatc
     6901 agtggctcac ggaacagcag accggtgaaa tctggcggca atatgtgccg catcagttcc
     6961 tgttcgccga aatactgacc acgctcggtc atattaatcg ttcagcaatt aacgtgttgt
     7021 tattgcgcca tgaacgcagt tctctgccgc tcggcaagtt tttggtcacc gaaggcgtta
     7081 tcagccagga aacgttggat cgcgtcctga caattcaacg cgaattacaa gtttcgatgc
     7141 aatcactatt actcaaagca ggtttaaaca cagaacaggt tgcgcaactg gagtccgaaa
     7201 atgaaggaga ataaccttaa tcgcgtcatc ggatggtctg gtttactgct gacgtcttta
     7261 ttgagtacca gcgcactcgc agacaatatc ggcaccagcg cagaagagct ggggctgagc
     7321 gattatcgcc attttgttat ttatccccgt ctcgataagg cgctgaaggc acagaaaaat
     7381 aacgacgaag caaccgccat ccgcgaattt gaatatatac accaacaggt gccggataat
     7441 attccgctga ctttatacct tgcggaagcc tatcgtcatt ttggtcatga tgaccgggcg
     7501 cggctgttgc ttgaggatca actgaaacgt cacccaggag atgcccgact tgagcgcagt
     7561 ctggcggcta ttccagttga agtgaaaagc gttacgactg ttgaagaact gcttgcccag
     7621 caaaaagcgg gcgacgctgc gccgaccctg cgttgtcgca gtgaagtcgg gcagaatgcc
     7681 ctgcggctgg cacaattacc tgtcgccaga gcgcaactga acgatgcgac gtttgctgca
     7741 tcgccggaag gaaaaacgct gcgaaccgat ctgctgcaac gggcaatcta cctgaaacaa
     7801 tgatcccagg cagatacgct atacaatgaa gcacgccagc agaacacatt aagcgcggca
     7861 gaacgccgtc agtggtttga cgtgcttctt gccgggcagc tggacgatcg gatcctggca
     7921 ctgcaatcac aggggatctt caccgatcct cagtcatata ttacttacgc gaccgcgctg
     7981 gcttatcgtg gcgaaaaagc acgcctccag cattatctca ttgaaaataa gccgctgttt
     8041 accacgggcg cacaagagaa aagttggctc tatctgttat ctaaatacag cgccaacccc
     8101 gttcaggcat tggcgaatta tacggtgcag tttgccgata accgccagta tgttgttggc
     8161 gcgacgctac cggtgctgtt aaaagaaggt cagtacgacg cagcgcaaaa actgctcgcc
     8221 accctccccg ccaatgaaat gcttgaggag cgttatgctg tcagcgtggc gacccgtaac
     8281 aaggctgaat ctctgcgtct ggcacgattg ctgtatcagc aagaaccgac aaatcttccc
     8341 cgcctggatc aactgacctg gcaactgatg cagaacgagc agtcacgcga agctgccgat
     8401 ttattgctgc aacgctatcc tttccagggc gatgcgcgtg tcagccagac tttaatggcg
     8461 cgactggcgt ctctgctgga aagtcatcct tacctagcaa cgccggcgaa ggtggcgatt
     8521 ttatcgaaac ccttaccgct ggcggagcaa cgtcagtggc aaagtcagtt gccgggtatt
     8581 gcagataatt gcccggcaat agttcgcttg ctgggcgata tgtcgccttc ctacgatgct
     8641 gccgcctgga accgtctggc aaagtgttat cgggacacgc tacccggtgt ggcgttgtat
     8701 gcatggcttc aggccgaaca acgacaaccg agcgcctggc aacatcgtgc ggtagcctat
     8761 caggcgtatc aggttgagga ctacgccacc gcactggcgg cctggcagaa aatcagtctt
     8821 cacgacatga gcaatgatga tctgctcgcc gccgccaaca ccgctcaggc agcgggaaat
     8881 ggcgcagctc gcgatcgctg gcttcaacag gcagaacaac gtgagctggg aaacaatgct
     8941 caatactggt ggctgcatgc gcgacgttat atcccagctc agccagaact cgcactgaac
     9001 gatctcacgc gctcaatcaa tattgcccct tctgccaact cttacgttgc gcgggcgaca
     9061 atttatcgcc aacgtcataa tgttccggcc gcggtgagtg atttgcgcgc cgcgctggaa
     9121 ctggaaccaa ataatagcaa cacccaggca gcgcttggtt acgccttgtg ggatagcggt
     9181 gatatcgcac agtcgcggga aatgctcgaa caggcgcata aagggcttcc ggacgatccg
     9241 gcactgatcc gacaactggc ctacgtgaac cagcgtctgg atgacatgcc tgcgacgcag
     9301 cactacgccc ggctcgtgat tgatgacatt gataatcagg cataacccca ctgaccccag
     9361 agcaaaatca gcaacgcttc aatttccgcc gtttgcatga ggaggtcggt cgccgctgga
     9421 cgttcagttt cgattcttcc atcggcttgc gttccggcgc aatgagtacc gctaacaata
     9481 atgtcggcgg tgcagcgcct gggaaaagct atcgtagcta cggacaactg gaagccgagt
     9541 accgcatcgg acgcaatatg ctgctggaag gcgacctgct ctcagtttat agccgcgtct
     9601 ttgccgatac cggagaaaac ggggtgatga tgccggtgaa aaatccgatg tccggcaccg
     9661 gtctgcgctg gaagccgctg cgcgatcaga tctttttcct cgccgtcgaa cagcagttgc
     9721 cgctgaacgg gcaaaatggc gcatccgata ccatgctgcg cgccagcgcc tcattcttta
     9781 atggcggcaa atacagcgac gaatggcacc cgaacggttc aggctggttt gcccaaaacc
     9841 tgtacctcga tgcggcgcaa tatatccgcc aggatattca ggcgtggacg gcagattatc
     9901 gcgtcagctg gcatcagaag gtagctaacg gacagactat tgagccttac gctcacgttc
     9961 aggacaacgg ctatcgtgat aaaggcactc agggcgcgca gcttggcggt gtcggggtcc
    10021 gctggaatat ttggaccggc gagacgcact acgacgcctg gccgcacaaa gtcagtctcg
    10081 gcgtcgagta tcaacatacc tttaaggcga ttaatcaacg taacggagag cgcaacaacg
    10141 cgtttctcac cattggagtg cactggtaaa tgcgtaagtt cattttcgta ttgctgacac
    10201 tgcttttggt cagccctttt tcctttgcga tgaaaggtat tatctggcaa ccacaaaacc
    10261 gagatagtca ggttaccgat acccagtggc aggggctgat gagtcagtta cgttttcaag
    10321 gcttcgatac cctggttttg caatggaccc gttacggcga tgcatttacc cagccagaac
    10381 agcgcacgtt attgtttaag cgtgccgcag ctgcgcaaca gactggcctg aagctgattg
    10441 tcgggctgaa cgccgatccg gaatttttta tgcaccagaa acagtcgtct gcagcgctgg
    10501 aaagctatct taatcgcctg ctggctgccg atctccagca agccagatta tggagcgccg
    10561 cgcctggcat aacgccggat ggctggtaca tcagcgcgga aattgacgac ctgaactggc
    10621 gcagcgaagc cgcccgtcaa cctttgctaa catggttaaa caacgcgcag cggctgatta
    10681 gcgatgtttc agcaaaaccg gtttatatca gtagtttttt cgccggaaac atgtcgcccg
    10741 atggctatcg ccaactgctg gaacaagtta aagcaaccgg agttaatgtc tgggtacagg
    10801 atggcagcgg cgtggataaa cttaccgctg aacagcgtga acgttatcta caggccagcg
    10861 ccgattgcca aagctccgcc ccagcaagcg gcattgttta tgaactgttt gtcgccggca
    10921 aaggcaaaac ctttacagcg aaaccgaaac cggacgcaga aattgcctcg ctgttagcga
    10981 aacgttcctc ttgcggtaaa gacatgctct atttctctct gcgctatttg cccgtcgcgc
    11041 acggcattct cgagtattaa atcccctcca ggtaagtcgg gtacgacctg gcttacctct
    11101 ttcgctctcg tcagaattcc atcaagatta attgctaaaa agcatgctaa agaaagaaga
    11161 tcgttatcgg cgaacgggcc gatcactcat gcaggaagca tagatccttt gagcggagtt
    11221 tctgatatct gaggagtgcg aaatgcaatt gagcagcagt gaaccttgcg tggtgatcct
    11281 gaccgaaaaa gaggtagagg taagcgtcaa taaccatgct acgtttaccc ttccgaaaaa
    11341 ctacctggcc gccttcgcgt gcaacaataa cgtcattgaa ctctcaacgt taaatcacgt
    11401 attaatcacc cacatcaacc gtaacatcat caacgattat ctgttgtttt taaataagaa
    11461 attaacctgt gtaaagccct ggtcgcggct agcaaccccg gttatcgctt gtcatagccg
    11521 tacaccggaa gtgttccggc tagccaccaa ccacagcaag cagcaatcca gcaaaccctg
    11581 cgaagcggag ttgacgcgcg cattactttt taccgtattg tctaactttc ttgagcaatc
    11641 gcggtttatt gccctactga tgtatatctt acgcagcagc gtccgcgaca gcgtctgccg
    11701 cattattcaa agcgatattc agcattactg gaatctgcga attgtcgcca gttcgctatg
    11761 tttaagcccc agcctgctca aaaagaaatt aaaaaacgaa aataccagct atagccagat
    11821 tgtcacagag tgtcgtatgc gttacgccgt acagatgtta ttgatggata acaaaaatat
    11881 cactcaggtg gcgcaattat gtggctatag cagcacgtcg tactttatct ctgtttttaa
    11941 ggcgttttac agcctgacac cgttgaatta tctcgccaaa cagcgacaaa aagtgatgtg
    12001 gtgaagggca aagcggaaac ggataagacg ggcataaatg aagaagaaat ggcgcgccct
    12061 gcaggattcg aacctgcggc ccacgactta gaaggtcgtt gctctatcca actgagctaa
    12121 gggcgcgttg ataccgcaat gcggtgtaat cgcgtgaatt atacggtcaa cccttgctga
    12181 gtcaatggct tttgatctgg ttgctgaaca agtgaacgac cgcgtctgat tttctgattt
    12241 atttcgctat agcggcaaac aaacgcacac cgctgcgcgt ctgaatcaag aaaacccgta
    12301 ttttcatgta tcaaagtaca atttaccgcc ctaacggaaa attgtccgct cctatgagac
    12361 tggtaactat gaaaccaacg tcggtgatca ttatggatac tcatcctatc atcagaatgt
    12421 ctattgaagt tctgttgcaa aaaaacagtg aattgcagat tgtcctgaaa acggatgatt
    12481 accgcataac catcgattat ctccgaaccc gtcctgttga tttaatcatt atggatatag
    12541 acttgcccgg aacagacggt tttaccttcc t
//
LOCUS       DAJTSA010000109        12348 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000109 DAJTSA010000000
VERSION     DAJTSA010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.109"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..247)
                     /gene="yefM"
                     /locus_tag="OO369_003331"
     CDS             complement(<1..247)
                     /gene="yefM"
                     /locus_tag="OO369_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR5983486.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            530..571
                     /gene="hisL"
                     /locus_tag="OO369_003332"
     CDS             530..571
                     /gene="hisL"
                     /locus_tag="OO369_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR5983487.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    547..670
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            717..1616
                     /gene="hisG"
                     /locus_tag="OO369_003333"
     CDS             717..1616
                     /gene="hisG"
                     /locus_tag="OO369_003333"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR5983488.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1622..2926
                     /gene="hisD"
                     /locus_tag="OO369_003334"
     CDS             1622..2926
                     /gene="hisD"
                     /locus_tag="OO369_003334"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR5983489.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2923..3993
                     /gene="hisC"
                     /locus_tag="OO369_003335"
     CDS             2923..3993
                     /gene="hisC"
                     /locus_tag="OO369_003335"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR5983490.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3993..5060
                     /gene="hisB"
                     /locus_tag="OO369_003336"
     CDS             3993..5060
                     /gene="hisB"
                     /locus_tag="OO369_003336"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR5983491.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5060..5650
                     /gene="hisH"
                     /locus_tag="OO369_003337"
     CDS             5060..5650
                     /gene="hisH"
                     /locus_tag="OO369_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR5983492.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5650..6387
                     /gene="hisA"
                     /locus_tag="OO369_003338"
     CDS             5650..6387
                     /gene="hisA"
                     /locus_tag="OO369_003338"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR5983493.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6369..7145
                     /gene="hisF"
                     /locus_tag="OO369_003339"
     CDS             6369..7145
                     /gene="hisF"
                     /locus_tag="OO369_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR5983494.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7139..7750
                     /locus_tag="OO369_003340"
     CDS             7139..7750
                     /locus_tag="OO369_003340"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR5983495.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="OO369_003341"
     CDS             complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="OO369_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR5983496.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="OO369_003342"
                     /pseudo
     CDS             complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="OO369_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="OO369_003343"
     CDS             complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="OO369_003343"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR5983497.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11944..12303)
                     /locus_tag="OO369_003344"
     CDS             complement(11944..12303)
                     /locus_tag="OO369_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983498.1"
                     /translation="MNFNSRLESRYSYELMKKESEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
BASE COUNT         3122 a         3131 c         3081 g         3014 t
ORIGIN      
        1 caataatgtc cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
       61 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgaa ttgtattctt
      121 cgagtgacat cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat
      181 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
      241 tacgcatgta aatctcctat tttgtacagt tcattgtaca atgataggtg ttaattaact
      301 atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct
      361 ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttc cccgctcatt
      421 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
      481 tggtttaggt taaaagacat cagttgaata aacattcaca gagattttta tgacacgcgt
      541 tcaatttaaa caccaccatc atcctgacta gtctttcagg cgatgtgtgc tggaagacat
      601 tcagatcttc cagtggtgca tgaacgcatg agaaagcccc cggaagatca ccttccgggg
      661 gcttttttat tgcgcggttg ttaacggttc agacaggttt aaagaggaat aagaaaatga
      721 cagacaacac tcgtttacgc atagctatgc agaaatccgg ccgtttaagt gatgactcac
      781 gcgaattact ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga
      841 tggcagaaaa catgccgatt gatattctgc gcgtgcgtga cgatgacatt ccctgtctgg
      901 taatggacgg cgtggtagac cttgggatta tcggcgaaaa cgtgctggaa gaagagctgc
      961 ttaaccgccg cgcccagggt gaagatccgc gctactttac cctgcgtcgt ctggatttcg
     1021 gcggctgccg cctttcgctg gcaacgccgg ttgatgaagc ctgggacggt ccgctctcct
     1081 taaacggtaa acgtatcgcc acctcttatc ctcacctgct caagcgttat ctcgaccaga
     1141 aaggtatctc ttttaaatcc tgcttactga acggttctgt cgaagttgcg ccgcgtgccg
     1201 ggctggcgga tgcgatttgc gatctggttt ccaccggtgc cacgctggaa gctaacggcc
     1261 tgcgcgaagt cgaagttatc taccgctcga aagcctgcct gatccagcgc gatggcgaaa
     1321 tggaagaatc taaacagcaa ctgatcgaca agctactgac ccgtattcag ggtgtgatcc
     1381 aggcgcgcga atcaaaatac atcatgatgc acgcaccgac cgaacgcctg gatgaagtca
     1441 tcgccctgct gccaggtgcc gaacgcccaa ccattctgcc gctggcgggt gatcaacagc
     1501 gcgtagcgat gcacatggtg agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga
     1561 aagcactggg tgccagttca atcctggtcc tgccgattga gaagatgatg gagtgatcgc
     1621 catgagcttt aacacaatca ttgactggaa tagctgtact gcggagcaac aacgccagct
     1681 gttaatgcgc ccggcgatct ccgcttctga aagcattacc cgcaccgtta acgatattct
     1741 cgataacgtg aaaacgcgtg gcgatgaggc cctgcgtgaa tacagcgcga agtttgataa
     1801 aaccacggtt accgcgctga aggtgtctgc tgatgagatc gccgccgcca gcgaacgcct
     1861 gagcgacgag ctaaaacagg cgatggcggt ggcagtaaag agtattgaaa ccttccacac
     1921 tgcgcaaaaa ctgccgccgg tagatgtaga aacgcagcta ggcgtgcgtt gccagcaggt
     1981 cacgcgcccg gtggcttcag tgggtttgta tattccgggc ggctccgccc cgctcttctc
     2041 aacggtatta atgctggcaa ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg
     2101 ctcaccgccg ccgattgccg atgaaattct ttatgcggcg cagctgtgcg atgtgcagga
     2161 cgtgtttaac gtcggcggcg cacaggccat tgccgcgctg gcgtttggta cggaatctgt
     2221 gccgaaagtg gacaaaatct tcgggccggg taacgccttt gtcaccgaag caaaacgcca
     2281 ggtaagccag cgtctggacg gtgcggcgat cgatatgccc gcaggcccgt cggaagtgct
     2341 ggtgattgct gacagcggcg ctacgccgga ttttgtggct tctgatttgc tttctcaggc
     2401 tgaacacggc cctgactcac aggtaatttt actgaccccc gatgctgata tggcgcgtcg
     2461 cgttgccgag gccgtcgaac gccaactggc agaactgccg cgtgccgaaa ccacccgcca
     2521 ggcactgaac gccagccgcc tgatcgtgac taaagattta gcgcagtgcg tggagatctc
     2581 caaccagtac ggcccggagc acctgatcat tcagacccgc aacgcccgcg aactggtcga
     2641 tggcatcacc agcgccggtt cggtatttct tggtgactgg tcaccggaat cggcaggcga
     2701 ctacgcctcc ggcaccaacc acgtgctgcc gacttacggt tacaccgcca cctgttccag
     2761 cctcgggctg gcagatttcc agaaacgcat gaccgtacag gaactgtcga aagaaggttt
     2821 ctccgcgctg gcttcgacca ttgaaacact ggccgccgcc gagcgcctga ccgcccacaa
     2881 aaatgccgtt actttgcgtg ttaacgtcct taaggagcaa gcatgagcac cgtgactatt
     2941 actgatttag cgcgtgaaaa cgtccgcaac ctgacaccgt atcagtcggc gcgtcgtctg
     3001 ggcggtaacg gcgacgtctg gctgaacgcc aacgaatatc ccacagccgt ggagtttaag
     3061 cttactcagc aaacgctcaa ccgctacccg gaatgccagc caaaagcggt gattgaaaat
     3121 tacgcgcaat atgcaggcgt aaaaccggaa caggtgctgg tcagccgtgg cgcggacgaa
     3181 ggtattgaac tgctgatccg cgctttttgc gaaccgggta aagacgccat cctctactgc
     3241 ccgccaacgt acggcatgta cagcgtcagc gctgaaacca ttggtgtcga atgccgcaca
     3301 gtgccgacgc tggaaaactg gcaactggac ttacagggca tttccgacaa gctggacggc
     3361 gtaaaagtgg tctatgtttg cagccccaac aaccccaccg ggcaactgat caatccgcag
     3421 gattttcgca ccctgctgga gctaacccgc ggtaaggcga ttgtggttgc cgatgaagct
     3481 tatatcgagt tttgcccgca ggcatcgtta gctggctggc tggcggaata tccgcacctg
     3541 gcgattttgc gtaccctttc gaaagccttc gcgttggcgg ggcttcgttg cggatttacg
     3601 ctggcaaacg aagaagtcat caacctgctg atgaaagtga tcccccccta cccgctctcg
     3661 acgccggttg ccgacattgc cgcacaggcg ttaagcccgc agggaatcgt cgccatgagc
     3721 gaacgggtgg cgcaaattat tacagaacgc gaatacctga ttgccgcact gaaagagatc
     3781 ccctgcgtgg agcaggtttt cgactctgaa accaactaca ttctggcgcg ctttaaagcc
     3841 tccagtgcag tgtttaaatc tttgtgggat cagggcatta tcttacgtga tcagaataaa
     3901 caaccctctt taagcggctg cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc
     3961 gtcattgacg ccttacgtgc ggagcaagtt tgatgagtca gaagtatctt tttatcgatc
     4021 gcgatggaac cctgattagc gaaccgccga gtgattttca ggtggaccgt tttgacaaac
     4081 tcgcctttga accgggcgtg atcccggaat tgctgaagct gcaaaaagcg ggctacaagc
     4141 tggtgatgat cactaatcag gatggtctgg gaacacaaag tttcccgcaa gcggattttg
     4201 atggcccgca caacctgatg atgcagatct tcacctcgca aggcgtgcag tttgatgaag
     4261 tgctgatttg tccgcacctg cccgccgatg agtgcgactg ccgtaagccg aaagtaaaac
     4321 tggtggaggg ttatctggct gagcaagcga tggatcgtgc caacagttat gtaattggcg
     4381 atcgcgcgac cgacattcaa ctggcggaaa acatgggcat taatggttta cgctacgacc
     4441 gcgaaaccct gaactggcca atgattggcg agcaactcac taaacgagac cgttacgccc
     4501 atgtagtgcg caacaccaaa gagacgcaga ttgacgtcca ggtgtggctg gatcgcgaag
     4561 gtggcagcaa gattaacacc ggcgttggct tctttgatca catgctggat cagatcgcca
     4621 cccacggcgg tttccgcatg gaaatcaatg tcaaaggcga cctctatatc gacgatcacc
     4681 acaccgtcga agataccggc ctggcgctgg gcgaagcgtt aaaaattgcc ctcggcgaca
     4741 aacgcggtat ttgccgcttt ggttttgtgc tgccgatgga cgaatgcctt gcccgctgcg
     4801 cactggatat ctctggtcgc ccgcacctgg aatataaagc cgagtttacc taccagcgtg
     4861 tgggcgatct cagcactgaa atgatcgaac acttcttccg ttcgctctct tacaccatgg
     4921 gcgtgacact acacctgaaa accaaaggta aaaacgatca tcaccgtgta gaaagcctgt
     4981 tcaaagcctt tggtcgtacc ctgcgccagg ccatccgcgt cgaaggcgac accctgccct
     5041 cgtcgaaagg agtgctgtaa tgaacgtggt gatccttgat actggctgcg ccaacctgaa
     5101 ctctgtgaag tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga
     5161 cattgtgttg ctggccgata aactgttttt acccggcgtc ggcaccgcac aagcagcgat
     5221 ggatcaggtg cgtgagcgcg agctgtttga tctcatcaaa gcctgtaccc aaccggtgct
     5281 gggcatctgc ttagggatgc aactcctggg acgacgcagc gaagagagca atggcgtcga
     5341 cttgctgggc atcatcgacg aagacgtgcc gaaaatgacc gactttggtc tgccactgcc
     5401 acatatgggc tggaaccgcg tttatccgca ggcaggcaac cgcctgtttc aggggattga
     5461 agacggcgcg tacttttact ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat
     5521 cgcccagtgt aattacggcg aaccgttcac cgcggcggta caaaaagata acttctacgg
     5581 cgtgcagttc cacccggagc gttctggtgc cgctggcgct aagttgctga aaaacttcct
     5641 ggagatgtga tgattattcc ggcattagat ttaatcgacg gcaccgtggt gcgtctccat
     5701 cagggcgatt acggcaaaca acgcgattac ggtaacgacc cgctgccgcg cttacaggat
     5761 tacgctgcgc aaggtgccga agtgttgcac ctggtggatc tgaccggggc aaaagatccg
     5821 gctaagcgtc aaatcccgct gattaaaacc ctggtcgcgg gcgttaacgt cccggtgcag
     5881 gttggcggcg gtgttcgtag tgaagaagac gtggcggcgt tactggaagc tggcgttgcg
     5941 cgcgtggtgg tcggttccac cgcagtgaaa tcaccggacg tagtgaaagg ctggtttgaa
     6001 cgtttcggtg ccgatgcgct ggtgctggca ctggatgtcc gtattgacga gcaaggcaac
     6061 aagcaagtgg cagtcagcgg ctggcaagag aactcgggcg tttcactgga acaactggtg
     6121 gaaacctatc tgcccgtcgg cctgaaacat gtgttgtgta ccgatatctc gcgcgacggc
     6181 acgctggcgg gatctaacgt ctctttatat gaagaagtgt gtgccagata tccgcaagtg
     6241 gcgtttcagt cctccggcgg cattggcgac attaacgatg ttgctgccct acgtggcact
     6301 ggcgtgcgcg gcgtaatagt tggtcgtgca ttactggaag gtaaattcac cgtgaaggag
     6361 gccatcgcat gctggcaaaa cgcataatcc cgtgtctcga cgttcgtgat ggtcaggtgg
     6421 tgaaaggcgt acagtttcgc aaccatgaaa tcattggcga tatcgtgcca ctggcaaaac
     6481 gctacgccga agaaggtgca gatgaactgg tgttctacga tatcaccgct tccagcgatg
     6541 gccgtgtggt agataaaagc tgggtatctc gcgtggcgga agtgatcgac attccgtttt
     6601 gtgtggcggg tgggattaag tctctggaag atgccgcgaa aattctttcc tttggcgcgg
     6661 ataaaatttc catcaactcc cctgcgctgg cagacccaac attaattact cgcctggccg
     6721 atcgctttgg cgtgcagtgt attgtggttg gtattgatac ctggtacgac gccgaaaccg
     6781 gtaaatatca tgtaaatcaa tataccggcg atgaaagccg cacccgcgtc actcaatggg
     6841 aaacgctcga ctgggtagag gaggtgcaaa aacgcggcgc cggagaaatc gtcctcaaca
     6901 tgatgaatca ggacggcgtg tgtaacggtt acgacctcaa gcagctgaaa aaagtgcgtg
     6961 aagtttgcca cgtcccgctg attgcctccg gtggcgcggg caccatggaa cacttcctgg
     7021 aagccttccg cgatgccgac gttgacggtg cgctggcagc ttccgtattc cacaaacaaa
     7081 taatcaatat tggcgaatta aaagcgtacc tggcaacaca gggcgtggag atcaggatat
     7141 gttaacagaa caacaacgtc gcgaactgga ctgggaaaaa accgacggtc tgatgccggt
     7201 gattgtgcaa cacgcggtat ctggcgaagt gttaatgctg ggctacatga acccggaagc
     7261 cttagacaaa accatcgaaa gcggtaaagt caccttcttc tcgcgcacta aacagcgact
     7321 gtggatcaaa ggcgaaacct cgggcaattt tctcaatgtg gtaagtattg ccccggactg
     7381 cgacaacgac acattactgg tgctggcgaa tcccatcggg ccaacctgcc acaaaggcac
     7441 cagtagctgc ttcggcaaca ccgctcacca gtggctgttc ctgtatcaac tggaacaact
     7501 gctcgccgaa cgtaaatatg ccgacccgga aacctcctac accgccaaac tgtatgccag
     7561 cggcactaaa cgcattgcgc agaaagtggg cgaagaaggc gtggaaaccg cactggcagc
     7621 aacggtacat gatcgctttg agctgaccaa cgaggcatct gacttgatgt atcacctgct
     7681 ggtgctgttg caggatcagg atctggattt aacgacagta attgagaacc tgcataaacg
     7741 gcatcagtga gttacgggct gagcaggtgt gatgttgttg tcacacccgg agccattgcc
     7801 cgatgcgcaa acttatcgtg cctacacatt atctcaacac acgtaggccg gataagatgc
     7861 gccagcatcg catccggcaa aaaaaacggg catggtgtca ccaccctgcc cgtttctctt
     7921 aaatgcacaa taatattact tcgcgttgta attacgcaga gcgttacgcc ccaacacaat
     7981 ccccgcgcca accatgccgc ccagaagcac tgccagaatc aaggtgattg cctttttcgg
     8041 actatcgcga cgaataggta acgtcggttt catcacatag cggtaagcat gaatatcaag
     8101 atcatcaaac tttaattttt caatatccaa caggttttga cgtgtctgat agtagtttgg
     8161 tgagaacacc aacggacgag tcgcttcatg cttaatcatc gactccagcg cttcgctccc
     8221 tagaaggaac aacgtatctt gcgtcacatc ttcagtctgc tgaacctgtg gctttgtcac
     8281 ctgcgcctga ttcgcatact gcaacgcttc ctgaatctga cggatacgca gatctttctg
     8341 ctcctgcgcg accacttcct gggttcttaa agagtcctgc aagtttttcc gtcccagagc
     8401 aatgttgtcc ttgagatcct tttctagctc ttgattcact ttatcatcaa cttgctgaat
     8461 gtattgggcc aacttcattt gtgcgccctc tgcagtttgc ccaacataag aaacagtcaa
     8521 tggtaattgc tggttcttaa cagaaggttc gatggtaagt ttttctggct cttcctgatt
     8581 atccagcgtt tctgctaatg cagagaaggc agaactgaag cgaccaatta acgtctcctg
     8641 caaatccgat actttcggtg cagcctgacc atagataaca ttcatggcat tgttatagcc
     8701 agcaatttgc cccacgtcgg gctgagtgat aattgctgtt gacgtccatt tctccttcgc
     8761 tactgccaaa taaccaatag ccagggcaat agccacaatg acggaaatga taattgtcat
     8821 cttgccacgc cacaactgca ctagtaaatc aatcaaatca atctgttccg ggtcatggtt
     8881 ttgcccagaa acattattat tttctactct catcattacc ctaactgacg gaaatttcca
     8941 agtccgcata gtttagcgat aatccccgtg aatgctatag gataagtgat aaaaataatg
     9001 aattattgcg gaggtaacaa aagacaatca gggcgtaaat agccctgata acaagatgtt
     9061 agtcgctgcc aaagagatcg cgggtgtaga ccttatctgc cacatcctta agctcttctg
     9121 ccatacggtt ggagataatg acgtcggctt gttgtttgaa ggtggcgaga tcacgttcca
     9181 ggcgagagtt gaagaatgag tcttctttca tcaccggctc gtagatgatc acttcaacgc
     9241 ctttcgcctt gatacgcttc ataatcccct gaatagaaga cgcacggaag ttatctgacc
     9301 cgctcttcat aatcagacga taaataccca ccacttgcgg tttgcatcga caattgccga
     9361 gatcaggtta ttcggcacag actggtagtt cgccagtaac tgcttggtat ctttcggcag
     9421 acaataacca ccataaccaa acgacggatt gttgtaatgg ttgccaatac gcggatcgag
     9481 acaaacgcct tcgataatct ggcgagtatt cagacctaaa ctttctgcat aactatccag
     9541 ttcattaaag tatgctacgc gcatcgccag ataggtatta gcgaaaagtt taatcgcttc
     9601 tgcttcagtg gagtcggtaa acagggtcgg gatattttgc ttaatcgcgc cttcctgtaa
     9661 caacgcagca aaacgttctg cgcgttctga acgctcaccg atgacaatac gtgacggatg
     9721 gagattatcg taaagggctt taccttcgcg gaggaattcc ggagagaaaa taatattttc
     9781 agtacgatat ttcttatgca tcgctgcggt aaaaccaacg ggaaccgttg atttgatgac
     9841 cataaccgca taaggattta tctcaactac gtctttaatt actgattcta cactggatgt
     9901 attgaaataa ttagttttag gatcatagtc ggttggagtg gcgatgatga cataatcagc
     9961 atcccggtag gcttcatttt tatctaatgt ggcattaaag tgtattttat ctgattgcaa
    10021 aaactgctga atttccttat caacaatagg agatatccga tcattcagca tagcaacgcg
    10081 tgacggtaaa atatctaatg ccacaacctc atgattttgt gcgattagaa gcccgtttga
    10141 caagcctaca tagccagtac cggaaatggt gattttcatt tcatgctctc agaattaact
    10201 taactgtgaa tcatgatgtt tttagcatcg tgataagatg ctaaaagttt taacgctacg
    10261 ttgtcagatg gtcaacgcaa cgaaaaacaa ttcagatttt ctttataaga atagctgatg
    10321 tttattaagt taatattaag cagtgaaatt tagtctataa gatatttggc aaaaaaaagc
    10381 ccggtgcaat atacgccggg cctcaatttt attgttggtt aaatcagatt aatccagcca
    10441 ttcagtatgg aacacacctt ctttatcaat gcgcttataa gtatgcgccc cgaaatagtc
    10501 acgctgtgcc tggatcaggt tcgcaggcag aaccgcggca cggtagctat cgtaataggc
    10561 aaccgcagcg gcgaaggtcg gaaccgggat accgttctgt actgcataag cgacgacatc
    10621 gcgcagcgcc tgctggtagt catcggcaat ttgcttgaag tacggggcca gcagcaggtt
    10681 agcgatctgc ggattttcgg cataagcatc ggtgattttc tgcaggaact gcgcacggat
    10741 gatgcagcca gcacggaaaa tcttcgcgat ttcgccgtag ttcagatccc aattgtattc
    10801 ttcagacgca gcacgcagct gagagaagcc ctgagcgtaa gaaacgattt tgcccagata
    10861 cagcgcactg cgaacttttt cgatgaactc agccttgtcg cctgctgact gagcttgcgg
    10921 accagagaga actttagatg cggcaacgcg ctgatctttc agagaagaga tataacgtgc
    10981 aaacacagac tcggtaatca gcgacagcgg ttcgccgaga tccagcgcgc tctggctggt
    11041 ccatttaccg gtgcctttgt ttgctgcttc atccagaatc acatcaacca ggtagttacc
    11101 gtcttcatct tttttggtga agatatcttt agtgatgtcg atcaggtagc tgctaagttc
    11161 accgttattc cactcggtga aagtctgggc cagttcttca ttagatagat tcaggccacc
    11221 tttgagcaga gaatacgctt cagcaatgag ctgcatatct ccgtattcaa taccattgtg
    11281 aaccatcttc acataatggc ctgcgccgtc ggcaccaata taagtaacgc atggctcgcc
    11341 gtcttctgcc acggcagcga ttttagtcag gataggcgca accagctcat aagcctcttt
    11401 ctgcccgcca ggcataatag aaggaccttt cagcgcgccc tcttcaccgc cggaaacacc
    11461 ggtaccgatg aagttaaagc cctctgctga aagctcacga ttacgacgaa tggtgtcctg
    11521 gaagaaggta ttaccaccat caatgatgat gtcaccttta tcgagatatg gtttcaggga
    11581 atcaatcgca gcatccgtgc ctgcacctgc tttcaccatt aacaggatgc gacgaggcgt
    11641 ttccagagat tcaacaaact ctttcaccgt atagtaagga gccagtttct tgcctggatt
    11701 ttcagcaatc acttcttccg tcttttcgcg ggagcggttg aaaatagaga cggtataacc
    11761 acgactttcg atgttgagcg caaggttgcg ccccatcact gccataccga ctacgccgat
    11821 ctgttgcttt gacattgttt actcctgtct tctatattat taaccgtcta ttaaccgtta
    11881 tattattaac cgtcgtgatt ttcatcttct aatcaaggaa tacatatggt tacagcatgc
    11941 ttcttacttt atagcgataa gtatgccatt gggtgtatcc ataagaactt tgaattttgg
    12001 atacttttct ggattaaaat tagcatagct tcgtttagat ataacaataa acgaactatt
    12061 aaaagatttc atcttctcta ttaaatcatc ctgtgggatt acctcacact cgggattact
    12121 aatagtccaa tcactatatc ttgctaatcc aaagacgtca ccaattgcca tccctttata
    12181 gaagtaaata ccatcctcca agcctaattg gataagatta tctccatata actcactata
    12241 ttcactttct ttcttcatca gttcatagct atagcgactt tctaagcgtg aattaaaatt
    12301 caagtattca gaataaaaat atttgaattc aaaatacaac gatcgccc
//
LOCUS       DAJTSA010000110        12219 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000110 DAJTSA010000000
VERSION     DAJTSA010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.110"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..492)
                     /locus_tag="OO369_003345"
     CDS             complement(<1..492)
                     /locus_tag="OO369_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5983499.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(523..1215)
                     /gene="fimC"
                     /locus_tag="OO369_003346"
     CDS             complement(523..1215)
                     /gene="fimC"
                     /locus_tag="OO369_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5983500.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="OO369_003347"
     CDS             complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="OO369_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5983501.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2458..3324
                     /gene="folD"
                     /locus_tag="OO369_003348"
     CDS             2458..3324
                     /gene="folD"
                     /locus_tag="OO369_003348"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR5983502.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3326..3538
                     /gene="ybcJ"
                     /locus_tag="OO369_003349"
     CDS             3326..3538
                     /gene="ybcJ"
                     /locus_tag="OO369_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR5983503.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3646..4155
                     /locus_tag="OO369_003350"
     CDS             3646..4155
                     /locus_tag="OO369_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5983504.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="OO369_003351"
     CDS             complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="OO369_003351"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR5983505.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5750..6244
                     /gene="ppiB"
                     /locus_tag="OO369_003352"
     CDS             5750..6244
                     /gene="ppiB"
                     /locus_tag="OO369_003352"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR5983506.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6247..6969
                     /gene="lpxH"
                     /locus_tag="OO369_003353"
     CDS             6247..6969
                     /gene="lpxH"
                     /locus_tag="OO369_003353"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR5983507.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7087..7596
                     /gene="purE"
                     /locus_tag="OO369_003354"
     CDS             7087..7596
                     /gene="purE"
                     /locus_tag="OO369_003354"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR5983508.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7593..8660
                     /gene="purK"
                     /locus_tag="OO369_003355"
     CDS             7593..8660
                     /gene="purK"
                     /locus_tag="OO369_003355"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR5983509.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8797..9690)
                     /locus_tag="OO369_003356"
     CDS             complement(8797..9690)
                     /locus_tag="OO369_003356"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR5983510.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9687..10334)
                     /locus_tag="OO369_003357"
                     /pseudo
     CDS             complement(9687..10334)
                     /locus_tag="OO369_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10337..11569
                     /gene="allC"
                     /locus_tag="OO369_003358"
     CDS             10337..11569
                     /gene="allC"
                     /locus_tag="OO369_003358"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCR5983511.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVQESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            11580..>12219
                     /gene="allE"
                     /locus_tag="OO369_003359"
     CDS             11580..>12219
                     /gene="allE"
                     /locus_tag="OO369_003359"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCR5983512.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2967 a         3158 c         3117 g         2977 t
ORIGIN      
        1 agcgctactc aacaacgcta tctgaggaat agtgatgttc aggcgcatcg ctgcaaaatc
       61 aaagttaatt gtggcatcag gtacagcttt cagtaaattg atgcatttat tgttttgctg
      121 ctgggctaac tcgggaaatg cactgctatt aaggccaatt cgttcaagga gtacctggtt
      181 aaaacagggc attaacccac ctgatttatc acctgtattt tctgttgtcg attcaaatac
      241 gatatcctgt gaaccaatga actcatcatt acgccagaga tccacccgat acacgcccgc
      301 aggttgatga ttacctctct caaagcgcga taaatcagca acggatgtgc cattctctaa
      361 taaaaatgcc gggttgaaat aactttccgc atagcttaaa aaggagggta aaatggcaag
      421 agatgaataa caaattccgg ccagacacca ggtatacctc tgcggaatat ccgtagtagt
      481 gggtattttc ataattagct ctgatattgt cagatgattc ggttagttta agttcacttc
      541 tctgaccggg gttactgaac cataatcatt aacggtctga aatttcagct ttccctgcac
      601 tcctgagggt aagggaattt gtgaactaac tctgggtgcc gccatagcat tccccaactt
      661 ttggctgccc acttgcaggt taacgagggt gacataaaaa ggtgatggat tatgaacatt
      721 gatatagtta ccggatcgcg aaaacttgag tgtgtccggc gcttctgcgg gtaattcttg
      781 taattgaatt ggacggagaa ataatttcat gcgcgataaa atcgccagtt gcaaaacatt
      841 cctgccgttc aatgcatttt tatctaccga agggatcgtc ttaacattca tccagaacag
      901 agactcacga tctgccgcca gcggtggacc ggtgtaaata atacgcaaag tattttcgct
      961 tttgggttca ctaacaaata gtggcggtgt aatgatgaat gacttttctt ttacaccgct
     1021 gctgttttca atccacgaat tgaccagaaa gagctcattg gtatggctat ttttaatcca
     1081 taccgcagtc tgtttagcat cagcgggata aataatacgc gtggcaccta atgcgatccc
     1141 tccggcagca tgggcgctgg ccgaaatgat taaataaaat atcatgagca tcaataactt
     1201 tattttagtc atcatagtta atacgccttg tgtattcctg acggcaaaaa taaggagtat
     1261 aaagaaatga ttgctttggc ttcttacaac acatcattcc cttttcattt aataaagcgg
     1321 catgatacaa agtgaacgtg gtttcgattt acattgccac cataccgctt cagacaaccg
     1381 gtacattact ttgttataag acgtgatata taattcaaaa caacgtggtt ttgattattc
     1441 gtatctcata ataaaagtcg cgtcagcatt tgcttgccct gctgatgcac ttgtaccggt
     1501 gcctttataa cgtgcagaaa aatgaagaac gttggtcccg gggatcaagt tctggttggt
     1561 tgagaagcta ttcccatcag gtttgagaat tgcggatgta ttatcaagta tttcaatacc
     1621 gacacccgtt gctgttgttg tatttgtact ggatacaatg gccaataaat tatcattgat
     1681 tgcatcagcc tgaccagaaa atgcaacggc agcattagcg gcaacaacag gatcgcagtc
     1741 gttcaactga atggtgaatg gaattaatgc agaggtatta ccaacagcat tgaaaatatc
     1801 ggtacgatat tgaccgagtg tgacaacctg gtctgccgag tcagtattca ctgaacaggc
     1861 agcattcacc agttcgcctt caaagtgaat tgtaccgcca tctacaacgg cagcataact
     1921 acccgtagcg gcgaatagtg cggcagccag cgcagacgaa ataaatctta atttcatata
     1981 tattccttca atctcattta tcgactccac atccgtatat aaccgattac tttatttaag
     2041 acactgatag tagtaaaatt cctttttatc ctctaagaat gtcttaattg aaaatatgca
     2101 ctctattcta aaaaatagag agccccgtta gatgaatact tccgcgcaaa atatattcac
     2161 cacaaatata gacctgaagc ggtaaattac caggctgaaa attcttttta tattgtcagg
     2221 tatttcttaa attatcttaa tccttaaaca aggaaataaa tcagttccag atttacaacg
     2281 ccatcatgta cgaaaaatga agctttcaat ctcagcgacc tcagcgacgg tgcgcctaac
     2341 cttcgcaaga ggtcgcttca cgcgataaat ctgaaacgaa acctgacagc gcgccccgct
     2401 tctgacaaaa tagacgcatc cccttcgacc tacgtaacag atggaatcct ctctctgatg
     2461 gcagcaaaga ttattgacgg taaaacgatt gcgcagcagg tgcgctctga agttgctcaa
     2521 aaagttcagg ctcgtattgc agccggactg cgggcaccag gactggccgt tgtgctggtg
     2581 ggtagtaacc ctgcatcgca aatttatgtc gcaagcaaac gcaaggcttg tgaagaagtc
     2641 gggttcgtct cccgctctta tgacctcccg gaaaccacca gcgaagcgga gctgctggag
     2701 cttatcgatg cgctgaatgc cgacaacacc atcgatggca ttctggttca actgccgtta
     2761 ccggcgggta ttgataacgt caaagtgctg gaacgtattc atccggacaa agacgtggac
     2821 ggtttccatc cttacaacgt cggtcgtctg tgccagcgcg cgccgcgtct gcgtccctgc
     2881 accccgcgcg gtatcgtcac gctgcttgag cgttacaaca ttgatacctt cggcctcaac
     2941 gccgtggtga ttggcgcatc gaatatcgtc ggtcgcccaa tgagcatgga actgttgctc
     3001 gcaggttgca ccaccaccgt gactcaccgc ttcactaaaa atctgcgtca tcacgtagaa
     3061 aatgccgatc tgttgatcgt tgccgttggc aagccaggct ttattcccgg tgactggatc
     3121 aaagaaggcg caattgtgat tgatgtcggc atcaaccgtc tggaaaatgg caaagttgtg
     3181 ggcgacgtcg tgtttgaaga cgcggctaaa cgcgcctcat acattacgcc tgttcccggc
     3241 ggcgttggcc cgatgacggt tgccacgctg attgaaaaca cgctacaggc gtgcgttgaa
     3301 tatcatgatc cacagggtga gtaacatggc gacattttct ttaggtaaac atccgcacgt
     3361 tgagctgtgc gacttgctga aactggaagg ctggagcgaa agcggcgcgc aggcgaaaat
     3421 cgcgattgcc gaaggccagg tgaaagtcga cggtgcggtt gaaacgcgca aacgctgcaa
     3481 aatcgtcgcc ggtcagacag tgagttttgc aggacacagc gtacaggttg ttgcctgatc
     3541 cctttcccgg cctgtatcaa caggccggtt ctcttttcag acttttgccc tctacgcccc
     3601 gttcatccat gctgatagca tcgacatttt gccgaagcga ccatcatgcc aaccgttatt
     3661 acgcacgccg ccgttcccct ttgcattggt ttagggttgg gttcgaaagg tctgttattt
     3721 gccggaatca tcctggcgat gctgcccgat gccgacgtat tgtcgtttaa atttggcgtt
     3781 gcttacggca atgtttttgg tcatcgcggt tttacccatt cgctggtgtt tgcgtttgtt
     3841 gtcccgctct tatgtgtgct gattggacgt cgatggttca gggctgggct gattcgctgc
     3901 tggctgtttt taaccgtctc gttgctatcg cacagcttac tggattcggt aaccactggc
     3961 ggtaagggcg ttggctggct gtggccgtgg tcagatgaac gctttttcgc tccctggcag
     4021 gtgattaaag tcgcgccgtt tgcattgtct cgttacacca cagcgtacgg gcatcaggtg
     4081 attatttccg aattgatgtg ggtgtggttg ccggggatgc tactgatggg aatgttgtgg
     4141 tggcgcagac gataaccgga tgcgaaaact cgcatccggc aattgcgcaa ttacttacga
     4201 cgccaggtgg tcccttgcgg gccatcttcc agcacgatcc ccatctcgtt aagacggtca
     4261 cgtgccgcat ctgccgccgc ccagtctttc gctttacggg catccagacg ctgttgaatt
     4321 aacgcttcaa tctcagccac ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct
     4381 tccggttctt gctccagcag gcccaataca gcggaaagtt tacgcaggtg agatgccatt
     4441 gcattcgccg ctgccatatc ttctgctttc agacggttca cttcacgcgc catatcaaac
     4501 agcacggaat aggcttccgg ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct
     4561 tcaaacgctt cgccaccggc aggcgcaacg gttttatctg tgccgcgcag cgcagtgtag
     4621 aggcgctcca gcgccgcacg cgcctgcttc aggttctctt cgctgtagtt cagctggctg
     4681 cgatagtggc ccgacatcag gaagtaacgc acggtttccg cgtcgtagta tttcagcaca
     4741 tcgcgcacgg taaagaagtt acccagcgat ttggacatct tctcgcggtc aaccatcacc
     4801 attccggagt gcatccagta gttcacatac tgaccatcat gggcacaggt ggactgcgcg
     4861 atttcgttct cgtggtgcgg gaacatcagg tctgaaccgc cgccgtggat atcaaagtgg
     4921 ttacccagct gcttgcagtt catggccgaa cattcaatgt gccagccagg acgacccgcg
     4981 ccccacggag acggccagct cggttcgccc tctttcgaca tcttccacag aacgaagtcc
     5041 atccggttgc gtttgtcatc aaccacgtca acgcgcgcgc cagcctgcag ctggtcgaga
     5101 tcctgacgcg acagcacgcc ataagttgga tcggtcggga cgtcgaacat cacgtcgctg
     5161 ttgtccgcca cataagcgtg acctttggcg atcagttgtt cagtgagttc aataatttct
     5221 gcgatatggt gcgtcgcgcg cggctccata tccgggcgca gaatgttcaa agcatcaaaa
     5281 tctttgtgca tttcggcgat catgcgatcc accagcgcca caaagctttc gccattttca
     5341 ttggcgcgtt tgatgatttt gtcgtcgata tcggtaatgt tgcgcacata cttcagttta
     5401 tagccgagga aacgcagata gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga
     5461 ccgatatgac agagatcgta aacggtgatt ccacacacgt acatgccgac ttccccggcg
     5521 tgaataggct taaattcctc tttttggcgt gtcagagtat tgaagatttt tagcatcgaa
     5581 gattccgttt agacatgtgt gggtaattga gttgcgtata atacccatat ttcccgcgcg
     5641 aatcagcata cattgcgtga tgatccgatc gtgcggttat gctataacac caccctatat
     5701 atgacccgaa ctgggttgaa gcaccaatca aacggaacag gatgcaaaaa tggttacttt
     5761 ccacaccaat cacggcgata ttgtcatcaa aacttttgac gataaagcac ctgaaacagt
     5821 taaaaacttc ctggactact gccgcgaagg tttttacaac aacaccattt tccaccgtgt
     5881 tatcaacggc tttatgattc agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac
     5941 caaagaaccg atcaaaaacg aagccaacaa cggcctgaaa aatacccgtg gtacgctggc
     6001 aatggcacgt actcaggctc cgcactctgc aactgcacag ttcttcatca acgtggttga
     6061 taacgacttc ctgaacttct ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc
     6121 tgaagtggtt gacggcatgg acgtggtaga caaaatcaaa ggtgttgcaa ccggtcgtaa
     6181 cggtatgcac caggacgtgc caaaagaaga cgttatcatt gaaagcgtga ccgttagcga
     6241 gtaatcgtgg cgacactctt tattgcagat cttcatctct gcgtggaaga accggcgatc
     6301 accgccggtt ttctgcgttt tttagcgggg gaagcccgca aggccgacgc gctgtatatt
     6361 cttggcgatc tgtttgaagc atggattggc gacgacgatc ccaacccact ccatcgccag
     6421 atggcggcag cgatcaaagc ggtgtccgat tccggcgttc cctgttattt cattcatggc
     6481 aaccgtgatt ttctgctcgg caaacgcttt gcccgtgaaa gcggcatgac attattgccg
     6541 gaagaaaagg tgctcgaact ttatggtcgc cgggtgttga ttatgcatgg cgacacgctg
     6601 tgcaccgatg acgcgggtta tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa
     6661 atgctattcc tcgccctgcc gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgcgcg
     6721 aacagcaaag aagccaacag cagtaaatcg ctggcgatca tggacgttaa ccaaaacgct
     6781 gtggtcagtg cgatggaaaa acatcaggtg caatggctaa tccacgggca tacccatcgc
     6841 ccggcggtgc atgaacttat cgccaaccag caacctgctt ttcgcgtggt actgggtgcc
     6901 tggcatacgg aaggttcgat ggtgaaagtc acggctgatg acgttgagct gattcatttt
     6961 ccgttttaaa aaacccacaa ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc
     7021 tctttccgtg ctattctctg tgccctctaa agccgagagt tgtgcaccac aggagtttta
     7081 agacgcatgt cttcccgcaa taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc
     7141 gactgggcta ccatgcagtt cgccgtcgaa atcttcgaaa tcctgaatgt cccgcaccac
     7201 gttgaagtgg tttctgctca ccgcaccccc gataaactgt tcagcttcgc cgaaagcgcc
     7261 gaagagaacg gttatcaggt gattattgcg ggcgcaggcg gcgcagcgca cctgccaggc
     7321 atgattgccg ccaaaacgct ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg
     7381 agcggtgtcg atagcctcta ctccatcgta caaatgccgc gcggcattcc ggtgggtacg
     7441 ctggcgattg gtaaagctgg cgcggcaaac gcggcattac tggcagcaca aattctcgcg
     7501 actcacgata aagagctaca ccagcgtctg aatggctggc gcaaagccca gaccgacgaa
     7561 gtgctggaaa acccggaccc gcgaggtgcg gcatgaaaca ggtttgcgtc ctcggtaacg
     7621 ggcagttagg ccgtatgctg cgtcaggcag gcgaaccgtt aggcattgct gtctggccgg
     7681 tcgggctgga cgctgaaccg gcggcggtgc cttttcaaca aagcgtgatt accgctgaga
     7741 tcgaacgctg gccggaaacc gcattaaccc gcgagctggc gcgtcatccg gcctttgtga
     7801 accgcgatgt gttcccgatt attgctgacc gtctgactca gaaacagctt ttcgataagc
     7861 tccacctgcc gaccgcgccg tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt
     7921 ttgatcgttt aggtgaactg gcgattgtta agcgtcgcac tggtggctat gacggtcgcg
     7981 gtcaatggag tttacgcgcc aatgaaaccg aacagttacc ggcagagtgt tacggtgaat
     8041 gtattgtcga gcagggcatt aacttctctg gtgaagtatc gctggttggc gcgcgcggct
     8101 ttgatggcag caccgtgttt tatccgctga cgcataacct gcatcaggac ggtattttgc
     8161 gcaccagcgt cgcttttccg caggccaacg cacagcagca ggcgcaagcc gaagagatgc
     8221 tgtcggcgat tatgcaggag ctgggctatg tgggcgtgat ggcgatggag tgttttgtca
     8281 ccccgcaagg tctgctgatc aacgaactgg ctccgcgtgt gcataacagc ggtcactgga
     8341 cacaaaacgg tgccagcatc agccagtttg agctgcatct gcgggcgatt accgatctgc
     8401 cgttaccgca accagtagtg aataatccgt cggtgatgat caatctgatt ggtagcgatg
     8461 tgaattatga ctggctgaaa ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc
     8521 gtccggggcg taaagtgggg catctgaatt tgaccgacag cgacacatcg cgtctgactg
     8581 cgacgctgga agccttaatc ccgctgctgc cgccagagta tgccagcggc gtgatttggg
     8641 cgcagagtaa gttcagttaa gcaggtgctc atttgattgc cggatgcggc gtaaacgcct
     8701 tatccggctt accgctccgg tacccattgt aggcctgata agacgcgtca agcgtcgcat
     8761 caggcatggt gcaccgattg ccggatgctc tcatcactac agcgaaatac aggtccccgc
     8821 ttcgcccgcc agcgtctctt caattcgcga taacgcccca atccacgcgg gtttactacg
     8881 gcttctgaca taaccactca ccgccgttac cttcggcccc atcgaaccat cggctttggc
     8941 aaatggcgct aactcatccg gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca
     9001 gttttcatat accgcatcag catcggtgag gatcaccagt ccatcggcat taatctgctc
     9061 ggcgagcaac gcagcggcga gatctttatc aatcaccgct tcactccctg ccccatcttc
     9121 cgtcacaggc acaccgccgc cgccactgca aatcaccaca tgcccctctt tgagcaacaa
     9181 ctcgatggct tcgctgtcga gaattttacg cggttgcgga gacgccacca cccggcgcaa
     9241 atatttacca tcacgtttca tctgccagcc gtaagccgct tccagtgcct cttgttcttc
     9301 tggctgataa accggaccaa taaatttctc tggctgcaaa aacgccggat catcaggcga
     9361 aacctcaatg cgcgtcagca ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct
     9421 ctgcgccagc atatagccaa tcatcccctg gctttccgcc accagtacat ccagcggata
     9481 cggttctacc tctttccacg ccagattctg taacgccagc agccccacct gcggcccgtt
     9541 gccgtgaaca atcgccagcc gataagaacg ggccaggcgt gccagcgcgg gtacagcact
     9601 ggcgatattg cgatattgat tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt
     9661 gcccccaaga gctacaacca gtgttttcat ggttttcctt gtaataattg ttggccaagc
     9721 cagaacccca gcagcgtatc tgcccctgac gtatgcccca gcgccagcag cgaatcaatc
     9781 gcaacagcgg tacgtttcgg gcaactcaga gcatggacaa agtgcaggag tggcgaggcg
     9841 aaatatcctt gcgcggcata acgtaaataa ctgacgctca ccgctgtggt aacgagttga
     9901 agattgtcgg aacaggcaaa aaacgggcga ccggcgcgcg catctaaagc gccataatac
     9961 caggccgcca gcagcattcc gctcaacgtg tcatcatgac ttggcgttaa tccggggcct
    10021 ttacccagcc agtgccgcca gtcggtctta acgccattga gcgcggcctg aaaacagtga
    10081 cgaaactggc gtaattcagc gggcagcgga tcgcttgccg ccatcgccag tggcccgaaa
    10141 agtccggttt cctccgcgcg ttgcatccat gcagctgcaa gcggttgagg atgcgaaggc
    10201 ggcgtaatac gcagcaaaca ataacgctgt ggctgtttaa ccgtgaaacg cccgaggcga
    10261 atcccttgag ccacaacctg tggtcgttca ttgccgcata atccaccgca taacgcatcg
    10321 aattgcgtgc ggcgaaatta cacattcccg tcaagctata gaagaaacgc tgccctggct
    10381 ttcctctttt ggcgctgacc caacgggtgg gatgacccgt ttactttatt cgccggaatg
    10441 gctggaaacc cagcagcaat ttaaaaaaag aatggcagca agtgggctgg aaacacgttt
    10501 cgatgaagtg gggaatttat acggtcgcct gagtggcacc gaatatccac aggaagtggt
    10561 tctgagcggt tcgcatatcg ataccgtggt taacggcggt aacctcgacg ggcaattcgg
    10621 cgcgctggcg gcgtggctgg caattgactg gttgaaaacg caatacggcg caccgttacg
    10681 tacggtcgaa gtggtggcga tggcagaaga agaaggcagc cgcttcccgt atgtcttctg
    10741 gggcagtaaa aatatctttg ggctggcgaa tcctgacgac gtgcggaata tctgtgatgc
    10801 caaaggaaat agttttgccg atgcgatgaa ggcttgcgga tttactctgc cgaacgcccc
    10861 actaactccg cgtcaggcta taaaagcctt tgttgaactg catattgaac agggctgtgt
    10921 gcaggaaagt aatgggcaat caattggcgt ggtgaatgca attgtcgggc agcgtcgcta
    10981 tacggtgacg ctgaacggcg aatcaaacca tgcaggcacc acgccgatgg gttatcgtcg
    11041 tgatacggtt tacgctttca gtcgcatttg ccatcagtcg atcgaaaaag cgaaaaagat
    11101 gggcgatccg ctggttctga cctttggcaa agtagagccg cgcccgaata cggtaaatgt
    11161 ggtgccgggt aaaaccacgt tcaccattga ttgtcgtcat accgacgctg ccgtgctgcg
    11221 cgatttcacc caacagttag aaaacgacat gcgggcgatt tgcgatgaaa tggacattgg
    11281 tattgatatc gatttatgga tggacgaaga acccgtgccg atgaataagg agctggtcgc
    11341 caccctgaca gaattgtgtg aaagagaaaa actgaattac cgggtgatgc acagtggtgc
    11401 cgggcacgac gcgcaaattt tcgcgcctcg cgtaccaacc tgcatgattt ttatccccag
    11461 catcaacggg atcagccata acccggcgga acgcaccaat attactgacc ttgccgaagg
    11521 ggtcaaaacg ttggcactca tgctttatca acttgcctgg caggaataag gagtcataaa
    11581 tgggatattt aaataacgtc accggttacc gcgaagattt actggctaac cgtgcgattg
    11641 ttaaacacgg taatttcgca ctgttaaccc cggatggtct gataaaaaat attattccgg
    11701 actttgaaaa ttgtgacgcg acaatcctct ccacgccaaa gctgggtgcc tcttttgttg
    11761 attatctggt cacactgcat caaaacggtg gcaaccaaca gggcttcggt ggcgaaggca
    11821 ttgaaacgtt cctgtatgtg atctctggaa atatcactgc caaagccgaa ggcaaaacat
    11881 ttgccttaag cgaaggtggc tatctttatt gcccaccagg ctccttaatg acgtttgtta
    11941 acgcccaggc cgaagacagc caaatctttt tatataagcg ccgctatgtt ccggtagaag
    12001 gccatgcacc gtggctgttt tctggcaatg ccagcgaact ggaacgcatt cactatgaag
    12061 gtatggatga tgtgatcctg ctggattttc tgcccaaaga gttaggtttt gatatgaaca
    12121 tgcatatcct ctcttttgca ccaggtgcca gccacggtta tatcgaaaca cacgttcagg
    12181 aacatggtgc ctatattctt tccggtcagg gggtttata
//
LOCUS       DAJTSA010000111        12150 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000111 DAJTSA010000000
VERSION     DAJTSA010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12150
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.111"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            126..995
                     /locus_tag="OO369_003360"
     CDS             126..995
                     /locus_tag="OO369_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR5983513.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1198..1551)
                     /locus_tag="OO369_003361"
     CDS             complement(1198..1551)
                     /locus_tag="OO369_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR5983514.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1689..3335)
                     /gene="groL"
                     /locus_tag="OO369_003362"
     CDS             complement(1689..3335)
                     /gene="groL"
                     /locus_tag="OO369_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR5983515.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3379..3672)
                     /locus_tag="OO369_003363"
     CDS             complement(3379..3672)
                     /locus_tag="OO369_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR5983516.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3948..5204
                     /gene="yjeH"
                     /locus_tag="OO369_003364"
     CDS             3948..5204
                     /gene="yjeH"
                     /locus_tag="OO369_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR5983517.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5220..5696)
                     /gene="fxsA"
                     /locus_tag="OO369_003365"
     CDS             complement(5220..5696)
                     /gene="fxsA"
                     /locus_tag="OO369_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR5983518.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6033..7469
                     /gene="aspA"
                     /locus_tag="OO369_003366"
     CDS             6033..7469
                     /gene="aspA"
                     /locus_tag="OO369_003366"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR5983519.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7587..8888
                     /gene="dcuA"
                     /locus_tag="OO369_003367"
     CDS             7587..8888
                     /gene="dcuA"
                     /locus_tag="OO369_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR5983520.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9004..9342
                     /gene="cutA"
                     /locus_tag="OO369_003368"
     CDS             9004..9342
                     /gene="cutA"
                     /locus_tag="OO369_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR5983521.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9318..11015
                     /gene="dsbD"
                     /locus_tag="OO369_003369"
     CDS             9318..11015
                     /gene="dsbD"
                     /locus_tag="OO369_003369"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR5983522.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11052..11627
                     /locus_tag="OO369_003370"
     CDS             11052..11627
                     /locus_tag="OO369_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5983523.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11734..11809
                     /locus_tag="OO369_003371"
     tRNA            11734..11809
                     /locus_tag="OO369_003371"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11767..11769,aa:Phe,seq:gaa)
BASE COUNT         2931 a         3139 c         2979 g         3101 t
ORIGIN      
        1 ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat tactaccaag
       61 cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa
      121 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
      181 tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt
      241 ctgtcatgga actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata
      301 aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa
      361 tggcagaaaa catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc
      421 gggttaacac gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc
      481 tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc
      541 gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct
      601 cactgttaga tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat
      661 atgacaccta ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc
      721 tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc
      781 gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc
      841 cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcagcga
      901 ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac
      961 aatccaccag caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg
     1021 gagccatcca gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa
     1081 acgccttatc cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc
     1141 ctgataagcg aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca
     1201 gttcgggcac ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa
     1261 cattccctgc gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg
     1321 gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga
     1381 aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg
     1441 ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc
     1501 cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc
     1561 cataatcaat gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga
     1621 tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaagtg
     1681 cagggcaatt acatcatgcc gcccatgcca cccatgccgc ccataccgcc agcagcgcct
     1741 aagtcagctg catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg
     1801 ccagccacag aagctgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata
     1861 cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg
     1921 ccgcctttaa cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga
     1981 cgcagcggag cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc
     2041 tgaccacgca ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca
     2101 accacgcctt cttctaccgc agcacgggtc gcgtgcaggg catcttcaac gcgtgctttt
     2161 ttctctttca tttcaacttc ggtagcagca cctactttga taactgcaac gccgcctgcc
     2221 agtttcgcta cgcgctcctg cagtttttca cggtcgtagt cagaagttgc ttcttcaatc
     2281 tgctgacgga tctgagcaac acggccctgg attgcagctt cttcacccac gccatcgatg
     2341 atagtggtgg tgtctttgtt gatcacaaca cgtttagcct gacccaggtc ttccagggtt
     2401 gctttttcca gctccatacc gatctcttca gagattacgg taccgccagt cagggttgcg
     2461 atatcctgca gcatagcttt acgacgatcg ccgaagcccg gagctttaac cgcagcaact
     2521 ttcacgatgc cacgcatggt gttaacaacc agagttgcta gcgcttcgcc ttcaacatct
     2581 tcagcgatga tcagcagcgg tttgcctgct ttggcaacag cttccagaac cggcagcatt
     2641 tcgcggatgt tggagatttt cttgtcagcc agcaggatga acgggctttc cagttctact
     2701 gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata
     2761 ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt gataacgcct
     2821 tctttaccga ctttgtccat cgcttcagcg atcagtttac ctacggtttc gtcggagtta
     2881 gcggagatgg taccaacctg agcaatcgct ttagaatcag agcacggtac ggacagcgct
     2941 ttcagttctt caactgcagc ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg
     3001 ttcatgcccg cagcaacagc tttcagacct tcagtgatga tagcctgagc cagtacggtt
     3061 gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc
     3121 tgcgcaccca tattttcgaa cttgtcttcc agttcgattt cacgagcaac ggaaacacca
     3181 tctttggtga tggtcggtgc accgaaagat ttatccagaa ctacgttacg gccttttgga
     3241 ccgagggtaa ctttcactgc atctgccagt acgtttacgc cgcgcagcat tttcacacga
     3301 gcgtcgttac cgaattttac gtctttagct gccattatct ttattcctta aattcgtatg
     3361 ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga atgtcgcttt cggacatgat
     3421 caacacttct tcattgtcga tcttctcgga tttcacaccg tagccatcgt tgaaaataac
     3481 gatgtcgcca actttcacgt ccagcggctt cacttcgcca ttttcaagga tacggccatt
     3541 gccgacagcc agcacttcgc cgcgggtgga tttagccgct gcagagccgg tcagaacgat
     3601 gccgccagca gatttagttt caacttcttt acgcttgacg atcacgcgat catgcaatgg
     3661 acgaatattc attgataact ctcctttgag aaagtccgta tctgttatgg gtgacgccgg
     3721 agcttacgtg gtttcccggc tggtgaccag agaaatgggg atgaggcttc gccccttcaa
     3781 gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct
     3841 cacatttcat cgcaatttct tcatcgccgt cataagcgaa tctgattgtg ctaccatcga
     3901 aaatctacgc agttgcccaa aatttgggcg caatcggata tcaacccatg agtggactca
     3961 aacaagaact ggggctggcc cagggcattg gcctgctatc gacgtcatta ttaggcactg
     4021 gcgtgtttgc cgttcctgcg ttagctgcac tggtagcagg caataacagc ctgtgggcgt
     4081 ggcccgtttt gattatctta gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc
     4141 actatcccag cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg
     4201 agcgagtcac cggctggctg tttttatcgg tcattcccgt gggtttgcct gccgcactac
     4261 aaattgccgc cggattcggc caagcaatgt ttggctggca tagctggcaa ctgttgttgg
     4321 cagaactcgg tacgctggcg ttggtgtggt atatcggtac tctcggtgcc agttccagtg
     4381 ctaatctaca aaccgttatt gccggactta tcgtcgcact gattgtcgct atctggtggg
     4441 cgggcgatat caaacctgcg agtatcccct tccccgcgcc aggaaatatc gaacttaccg
     4501 ggttatttgc tgcgttatca gtgatgttct ggtgttttgt cggtctggaa gcatttgccc
     4561 atcttgcctc ggaatttaaa aatccagagc gtgattttcc tcgtgctttg atgattggtc
     4621 tgctgctggc aggattagtc tactggggct gtacggtagt cgtcttacac ttcgacgcct
     4681 atggtgaaca aatggcggcg gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg
     4741 taggagcgtt atggattgcc tgcgtaattg gctatctggc ctgctttgcc agtcttaaca
     4801 tttatataca gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat cctgaccact
     4861 acctggcacg cctctcttct cgccatatcc cgaataatgc cctcaatgcg gtgcttggct
     4921 gctgcgtggt gagcacgttg gtaattcatg ccttagagat caatctggac gctcttatta
     4981 tttatgccaa tggcatcttt attatgattt atctgttatg catgctggca ggctgtaaat
     5041 tattgcaagg tcgttatcga ctactggcgg tggttggcgg gctgttatgc gttctgttac
     5101 tggcaatggt cggctggaaa agtctctatg cgctgatcat gctggtgggg ttatggctgt
     5161 ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac ataatccggc gtttcgacat
     5221 taatcctggc gatcgtcttt atgatcaagg cggtcgcggt catcatcctt tcgctgatac
     5281 tcaccatcaa aagtattacc gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga
     5341 gaaaagcgca aatgtggcat caacttcact gtcagatgct tttgcaccgg cggcaataaa
     5401 agcagaagac cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa accggcaata
     5461 atcagcgaaa cacttttaat catctccgcc gcagggtttt cacccgccgc cattttttgc
     5521 tgcatcagca cgaaattctt aaagccctgg ttacgtacca gtgacatacc gataactgac
     5581 gtgaatataa ccagcacgag ggtcagcaat acccccaata catgggcaac ctgaataaag
     5641 attgaaatct caatatagac ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt
     5701 ttctcctgta atagcagccg gttaaacccg gctacctgaa tgggttgcga atcgccttca
     5761 gcttatattg tggtcattag caaaatttca agatgtttgc gcaactattt ttggtagtaa
     5821 tcccaaagcg gtgatctatt tcacaaatta ataattaagg ggtaaaaacc gacacttaaa
     5881 gtgatccaga ttacggtaga aatcctcaag cagcatatga tctcgggtat tcggtcgatg
     5941 caggggataa tcgtcggtcg aaaaacattc gaaaccacat atattctgtg tgtttaaagc
     6001 aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa caacattcgt atcgaagaag
     6061 atctgttggg taccagggaa gttccagctg atgcctacta tggtgttcac actctgagag
     6121 cgattgaaaa cttctatatc agcaacaaca aaatcagtga tattcctgaa tttgttcgcg
     6181 gtatggtaat ggttaaaaaa gccgcagcta tggcaaacaa agagctgcaa accattccta
     6241 aaagtgtagc gaatgccatc attgccgcat gtgatgaagt cctgaacaac ggaaaatgca
     6301 tggatcagtt cccggtagac gtctaccagg gcggcgcagg tacttccgta aacatgaaca
     6361 ccaacgaagt gctggccaat atcggtctgg aactgatggg tcaccagaaa ggtgaatatc
     6421 agtacctgaa cccgaacgac catgttaaca aatgtcagtc cactaacgac gcctacccga
     6481 ccggtttccg tatcgcagtt tactcttccc tgattaagct ggtagatgcg attaaccaac
     6541 tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga catcctgaaa atgggtcgta
     6601 cccagctgca ggacgcagta ccgatgaccc tcggtcagga attccgcgct ttcagcatcc
     6661 tgctgaaaga agaagtgaaa aacatccaac gtaccgctga actgctgctg gaagttaacc
     6721 ttggcgcaac agcaatcggt actggtctga acacgccgaa agagtactct ccgctggcag
     6781 tgaaaaaact ggctgaagtc actggcttcc catgcgtacc ggctgaagac ctgatcgaag
     6841 cgacctctga ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga
     6901 agatgtccaa aatctgtaac gacctgcgct tgctctcttc tggtccacgt gccggcctga
     6961 acgagatcaa cctgccggaa ctgcaggcgg gctcttccat catgccagct aaagtaaacc
     7021 cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt catcggtaac gacaccactg
     7081 ttaccatggc agcagaagca ggtcagctgc agttgaacgt tatggaaccg gtcattggcc
     7141 aggccatgtt cgaatccgtt cacattctga ccaacgcttg ctacaacctg ctggaaaaat
     7201 gcattaacgg catcactgct aacaaagaag tgtgcgaagg ttacgtttac aactctatcg
     7261 gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa
     7321 tctgtgccga aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg
     7381 aagcggaact tgacgatatt ttctccgtac agaatctgat gcacccggct tacaaagcaa
     7441 aacgctatac tgatgaaagc gaacagtaat cgtacagggt agtacaaata aaaaaggcac
     7501 gtcagatgac gtgccttttt tcttgtgagc agtaacttaa aaataacaat ctaatatcaa
     7561 cttgttaaaa aacaaggaag gctaatatgc tagttgtaga gctcatcata gttttgctgg
     7621 cgatcttctt gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg
     7681 tgctggttct tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca
     7741 ttatcatggc ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc
     7801 tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac
     7861 cgatcgtgac ctatttcctg actatctttg ctggtactgg caacatctct ctggcgacac
     7921 tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctctcta
     7981 ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt
     8041 acatgtcttc cgtgatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca
     8101 tcccgtccac cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact
     8161 ccaaactctc tgacgatccg atttatcgca agcgtctgga agagggcttg gttgaactgc
     8221 gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc
     8281 tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg
     8341 aaaaaccgct gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc
     8401 tgaccaccgt tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag
     8461 caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt
     8521 ccaacaacat cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc
     8581 tgctggccgt catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa
     8641 aagcactgat gccgatggct ctggcactga acgtttcacc gctgaccgct gttgcttctt
     8701 tcgctgcggt gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac
     8761 agatggatga cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca
     8821 tcccgggtac tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca
     8881 tgctgtaatg atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc
     8941 cttcccccgt ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg
     9001 tttatgcttg atgaaaaaag ttcgaatacc acgtctgtcg tggtgctatg taccgcaccg
     9061 gatgaagcga cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc
     9121 gcgaccttga tccccggcgc tacctctctc tattactggg aaggtaagct ggagcaagaa
     9181 tacgaagtgc agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc
     9241 ctgaagtctc atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga
     9301 gacacagatt acctctcatg gctcaacgca tctttacgct gattctgcta ctttgcagca
     9361 cttccgtttt tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc
     9421 aagcctttgc ttttgatttt cagcaaaacc aacatgacct taatctgacc tggcagatca
     9481 aagacggtta ctacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg
     9541 ccgacgagca gctgccgcaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga
     9601 tttaccgcga tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt
     9661 taactgtcac ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa
     9721 ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc
     9781 agcaagagca gcacaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta
     9841 ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc
     9901 tgggtggtaa acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc
     9961 aggggatggc actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt
    10021 tccaggcggc gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc
    10081 tggcgatgtc aatgtttggc ttgtttactc tacaactccc ctcttcgctg caaacacgtc
    10141 tcacgttgat gagcaatcgc caacagggcg gctcacctag cggtgtgttt gttatggggg
    10201 cgattgccgg actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgctgt
    10261 atatcgccca aagcgggaac atgtggccgg gcggcggcac gctttatctc tatgcgttgg
    10321 gcatgggcct gccgctgatg ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg
    10381 gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct
    10441 tcctgctgga gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg
    10501 tcgcattctt tggctgggcc tttatcacca gcctacaggc taaacgcggc tggatgcgcg
    10561 tggtgcaaat aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg
    10621 catttggtgc gacgcatacc gcgcaaactc aaacgcatct caactttaca caaatcaaaa
    10681 cggtagatga gttaaatcag gcgctcgttg aagccaaagg caaaccggtg atgttagatc
    10741 tttatgccga ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc
    10801 aggtgcaaaa agcgttagca gacacggtct tacttcaggc caacgtcacg gccaacgacg
    10861 cacaagatgt ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt
    10921 ttgacggaca aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa
    10981 ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg
    11041 aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac
    11101 aagggattgc caacaccacg ctggagatgg ttgctgaacg tgtggattat ccactggacg
    11161 agctacgccg cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta
    11221 gccaacagat agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac
    11281 aaaagctgct ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg
    11341 gctgtctatt tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac
    11401 tggccgatca gcaaaaaagc gctgcctacg atttcaccca cgaactgtta accacgctgg
    11461 aagttgacga tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa
    11521 gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac tgcacatcgg ctggcggaag
    11581 atatcctgcg cttcgcccgc tgccgccagg gtggtgcatt gacctgacag aaacacagaa
    11641 aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg
    11701 agatgtgcag attacggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag
    11761 gggattgaaa atccccgtgt ccttggttcg attccgagtc cgggcaccaa attcatatca
    11821 acggacctcc acggaggtcc gtttttcgtt tcaggacgcc acgatttaag cgttctgcct
    11881 ccaaatcaat tctaccgaac tcaaccagat tctccccaca tcaccagcaa tttgcgagca
    11941 tatcccaatt tgggaaaatt tgttcctgag ctatagcact aactgacgtg aaatgtcgtg
    12001 cggccccgtg atgctgttga acgtcaaatg acgtcatcag gagcgtaacg tatccataaa
    12061 gcacaacacc ggtctgaacg ccgactgatg agattttctg aatgagaaca aagagaaatg
    12121 tgtcagtccg cttgctcatg caaagactaa
//
LOCUS       DAJTSA010000112        12112 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000112 DAJTSA010000000
VERSION     DAJTSA010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.112"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..370
                     /locus_tag="OO369_003372"
     CDS             <1..370
                     /locus_tag="OO369_003372"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983524.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="OO369_003373"
     CDS             complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="OO369_003373"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR5983525.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="OO369_003374"
     CDS             complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="OO369_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR5983526.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="OO369_003375"
     CDS             complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="OO369_003375"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR5983527.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="OO369_003376"
     CDS             complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="OO369_003376"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR5983528.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3448..5049)
                     /gene="tap"
                     /locus_tag="OO369_003377"
     CDS             complement(3448..5049)
                     /gene="tap"
                     /locus_tag="OO369_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCR5983529.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5095..6756)
                     /gene="tar"
                     /locus_tag="OO369_003378"
     CDS             complement(5095..6756)
                     /gene="tar"
                     /locus_tag="OO369_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCR5983530.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6901..7404)
                     /gene="cheW"
                     /locus_tag="OO369_003379"
     CDS             complement(6901..7404)
                     /gene="cheW"
                     /locus_tag="OO369_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR5983531.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7425..9389)
                     /gene="cheA"
                     /locus_tag="OO369_003380"
     CDS             complement(7425..9389)
                     /gene="cheA"
                     /locus_tag="OO369_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCR5983532.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9394..10320)
                     /gene="motB"
                     /locus_tag="OO369_003381"
     CDS             complement(9394..10320)
                     /gene="motB"
                     /locus_tag="OO369_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR5983533.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10317..11204)
                     /gene="motA"
                     /locus_tag="OO369_003382"
     CDS             complement(10317..11204)
                     /gene="motA"
                     /locus_tag="OO369_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR5983534.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11331..11909)
                     /gene="flhC"
                     /locus_tag="OO369_003383"
     CDS             complement(11331..11909)
                     /gene="flhC"
                     /locus_tag="OO369_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR5983535.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11912..>12112)
                     /locus_tag="OO369_003384"
     CDS             complement(11912..>12112)
                     /locus_tag="OO369_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCR5983536.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2782 a         3388 c         2933 g         3009 t
ORIGIN      
        1 cactacgcta atgagttggg atgccgtgct ctatgatcta cgtagtgaaa aaattatcag
       61 taaagataat tacttgaccg acttaaaccg cagacatctt gaacttgttc ttcatactaa
      121 ccccaatcca agcggttcgg taacgaagat tttacgcact atttttacta agcaggtaaa
      181 gagtttaggg ccggaactct gtcatttctt gcaccatgaa ctgacgaaaa aacgttatga
      241 agtatggcat cactacgatg tactgcacta ccagtcatcg tcattttctc gttcacaatt
      301 taatgcattg ataaatttac tggcgcaaca atcacgcttc gctgatctaa aattatcaaa
      361 ctacaaataa ccatcctcca cgtactccct gaacgatacc cgccaccctg ctatcggctt
      421 ggcatcatcg cgcaggccga agttcaggca atacaaatca aaatccaaga ctatccaaca
      481 aatcgtccac ctgatcctga ctggctacca cgccggcttt gctggtatcg acctgaggtc
      541 cattaagcaa actctggttt tcacgttttg gacgcgactc ctgttccggg atgttttcca
      601 acagcaccat cagcaactgg cgttcgatct cctgaatgac atccatcatc cgcttaataa
      661 cctgcccggt gagatcctga aaatcctgcg ccatcatgat ttccagcagt tgcgcgttag
      721 taaagctggt atgcgcgggt acatctgcca gaaattgtcg tgtatctgtt accagttcac
      781 gggcgtcggc aaggtcaatc ggatcggcaa accagtcatc ccaacgttgg gttaacgctt
      841 ttgctgattt ctccatttga tcctgatgcg gttgtgacgc ctcaacactg ttcagcgccc
      901 gctccgcagc ctgggcggtc atctgcacaa catagtacaa acgatcgcgc gcatcgggga
      961 tggcttccgc cgcttcggca atggcctgaa ccagccccag ttcccgcaaa ctgtcgcgca
     1021 gcatatgcgt caggctgccg atgcgcgcaa tgatatcgcc agctgaatgc tcgtcagcag
     1081 gtttgattga tggttgcatc atagtcgcat cctcacatgc ccagtttctc aaagattttg
     1141 ttgagttttt cctccagcgt cgcggcggta aatggcttca ccacatagcc actggccccc
     1201 gcttgcgccg cagcaatgat gttctctttc ttcgcttctg cagtcaccat taacactggc
     1261 aatgccgaca tcgcgccatc cgcacgaatt gttttcagca attccaggcc atccatattg
     1321 ggcatgttcc agtcggagat aacaaatcca taaccgcctg cctgcaactt attgagagcg
     1381 tcgacaccat cttccgcttc ctcaacatta ttgaatccca gctctttcag caggttacgc
     1441 actatgcgtc gcatggtgga aaagtcatcc acaaccaaaa atttaagttc tttatccgcc
     1501 atttcacact cctgatttaa atacgtatcg cctgtccggc actaattgtt gccaacattt
     1561 gctggcttac ctggctaaga tcgaccactt cgcagacacc acccatattg atggcctcgc
     1621 gcggcatgcc gaacaccacg caacttgctt cgttttgcgc aagggtccat gcccccgcct
     1681 gacgcatcgc caacattccc gccgcgccgt cgttgcccat accggtcagg atcaccccaa
     1741 ccgcattacg ccccgcctgt ttggcgacag aatggaacaa cacatctacc gaaggccgat
     1801 gacggttaac cgccgggccg tcgtgaattt tgatttgata atttgcgcca ctacgcgcca
     1861 gttccatatg ccgatcgccc ggcgcaatat aggcatgccc cggcaagaca cgttctccgt
     1921 cttcggcttc tttaaccccg atctggcaaa gcttattaag tctgtcggca aaagagcggg
     1981 tgaaaccggg cggcatatgt tgggtaatta acaatgccgg gctggaaagc ggcaacggtt
     2041 gcagtacgtg acgaattgcc tcagttccac ccgttgaagc accaatcgca atcagttttt
     2101 cagaactcaa caacggccct gccttcagcg ttgtcggtgc tgacaatggc ttatgtgctg
     2161 caaggctcgc ctttgctgcc gtacgcacct tttcagcaat catttcgcta tacgccagca
     2221 ttccttcgcg aatacccagt tgcggtttgg tgacaaaatc tattgccccc agctccagcg
     2281 cgcgcagcgt gacttctgat cctttgccgg tcaaggaaga aaccatcaca acgggcattg
     2341 gacgcaaacg cattaatttt tcgaggaaat ccagtccgtc catccgcggc atttcaacat
     2401 ccagcgtcag cacatcggga ttgaatttct taatcaagtc acgcgcaacc agcggatcag
     2461 gcgcggtcgc caccatttcc atgtcgctat ggctgttgat gatttctgtc atgatctggc
     2521 gcatcagtgc cgaatcatcg acagataaca ccctgatttt gctcatcgtt aatccttact
     2581 tagcgcatac accgtctgac cacgcagcgt gaagcggcgc tcgaggtggc taaagttttc
     2641 agagtgaccc gcaaacagca atccgtcggg tttaaggagc ggaacaaagc ggcgcaaaat
     2701 ctcctgctgg gtagtttgat cgaagtagat catgacgtta cgacagaaga tcgcatcaaa
     2761 cggccccggc acggtgtact gtttcgccag taaattcagc ggggcaaaat caacatagtt
     2821 cgccagctcc tgacgcacgc gtaccagccc ttcatgcggt cccgtccctc gcatgaaata
     2881 ccgttgcagt tgctgcggcg tcaggttttt caactcttca tggcgataga taccgcttct
     2941 ggctttttcc agcacttcgg tgtcgatatc actggcaaac actttccagc gtccgggcac
     3001 ggtgcccaat gtgtcagcca gagtcatcgc aatgctgtac ggctcttcgc cggtcgaagc
     3061 cgccgcgctc catacgcgat cctcgccaga acggcgacgt gcgtgatccg cgagcagagg
     3121 gaaatgatga gcctcacgga aaaatgccgt cagattcgtg gtcagcgaat tgataaacgc
     3181 ctgccactca ccgctgtgct gattagattc cagcaagttc agataatgac cgaaatccgt
     3241 cagtcccaga gaacgcaaac gacgaaccag tcggttgtaa accatgtcgc gtttatggtc
     3301 agccagaacg atcccggctc gttgatagat caattgactt atccgccgaa aatgcgcgtc
     3361 ggaaagcgcc aggcgctcgg tcatctgtaa caataaagac gtttgcccac agggcagaga
     3421 tgaagtcata gcgccttctc aatcacttca ggataccact ggcgcaattt gtaactgcgc
     3481 cgactcatgt cgtgctactt catgttcttc aagggtaaat accgccacac gcgacgaaag
     3541 atggtcggcc tgattcgcca gttgttccgt tgccaccgcc gcttcttcta ccagcgagga
     3601 gttctgctga gtcacctgat ccatctggct gacagcctgt gcaacctgct caatcccccg
     3661 ccgttgttct tccgacgccg aagcaatttc tcccataatg tcgttcacgc gggtcaccga
     3721 actgacaata tcggtcatgg tcgcggcggc gttattcacc agtttcgaac cctgctggac
     3781 acgattgact gactcttcga tcagcccttt gatctctttt gctgcctgcg cgctacggct
     3841 ggcaagattg cgcacttcac ctgccactac cgcaaaacca cgcccctgct ctccggcgcg
     3901 agccgcttcc actgccgcat tcagggccag aatattggtc tggaaagcaa ttccgtcgat
     3961 aacgctgata atgtcgccaa ttttttgcga gctggtggcg atctcctgca tggtgtgagt
     4021 catggtactg acctgaacac cgcccgcctg cgccgttgtc gcggcatttt ttgccagttc
     4081 cgacgcctgt cgtgcgttat cggcgttttg ccctaccgtg gcggttaatt gctccatact
     4141 ggcggccgtt tgtgccagcg atgccgcctg ctgttcggta cgacttgaga gatcgttatt
     4201 gcctgcgaca atctccgcga tcccaatgtg catctcatgg cttcccttac gcacatcact
     4261 caccgtccca cgcaaagcct gctgcatggt cttcaggctg gcaaaaatgg cggtgatctc
     4321 attacgacca tataccgcaa tcggacgcgc cagattaccc gcagcaatgc tgtcaaaatg
     4381 gctaccgata atggccagtg gttgaacaat catcttgcgc gtccaccaca gcgcactgct
     4441 gatgtagatc gccgcaacaa taatcatgct gataaacacc agcgccgaaa tctgatagtt
     4501 acgctggctt tgcgcactgg cggcttccag cacatggttg atctccagct gccaggcctc
     4561 aaagttaacg tcaaacgcat tctgtgatcc ctgcaccggc gcagtgagga aatccgaaag
     4621 ttgattactt tcaagccagg tcgcctgatg ttcgagatcg ttgtgccagc gggcaaaact
     4681 tttctccgtc tctttttgca atgccctgac gtgctcgttg cccgcagtca tcgccataaa
     4741 acttttaaac agcgtggtgg attgcgtcag actggcgcgc gccgtcgtca tcaacgtttt
     4801 aatgtcatcc gccggatagc taagcgctgt cagagtgcct gctttgttca gcgcggtact
     4861 ggcctgtaac attaccgccc gagtttgcgc taatgccgca cgttgctgat tgctctgctc
     4921 gccgtgattc agtcgttgca aatcgtcgcg aaatgcccaa aaagacatgc cgttactgcc
     4981 aatctgcaag atcccgcaga gaatcaaaat taaaaacagc gtggtcgaaa ttcgaatacg
     5041 attaaacatc aacgctcccc atcaggcggc aatgaccgcg ttagtaaaaa ctcgtcaaaa
     5101 tgtttcccag tttggatctt gttcagtaat tcgcagtcgt ggctgtgccg gtggttgctc
     5161 actggcagga cgggatggtg tttgcggttt attggtgagt gggctggctg ccagacggaa
     5221 cgcggaaacc gcttgcgtta aacgactcgc ctgttcttcc agcgcagctg cggcggcagc
     5281 tgattcctgc accagcgatg cgttctgttg cgtgacgcga tccatttccg aaaccgccaa
     5341 tgcgacttga tcgatgccac ggctctgttc atccgatgcc gatgcaatct cgcccataat
     5401 gtcagtcacg cgagtgacag cattgacgat attgttcatt gtttccccgg cgctttcgac
     5461 cagcaccgaa ccggtatcaa tgcgcgagac ggagtcttca atgagggctt tgatctcttt
     5521 tgccgccagg gcgctgcgac tggcaagatt acgcacttca cccgccacca cggcaaaacc
     5581 acggccctgt tcacccgcac gcgcggcttc aaccgcggca ttcagcgcga ggatattagt
     5641 ctggaaggca ataccgtcga taacgctgat aatgtcggca attttcttcg aactatcggc
     5701 gatctcatgc atcgttttca ctacgccatc caccactttg ccgccgtgct gggcggtgtc
     5761 ggaggcactt tgcgccagtt gcgaggcctg gcgggcgtta tcggcgtttt gcttcactgt
     5821 cgcggtgagc tgctccatgc tggcggcagt ttcttccagc gcggatgcct gctgttcagt
     5881 acgggaggaa agatcggtgt tgcccgccgc aatttcacgg gtaccggcat agatggcatc
     5941 tgaaccttcg cggacatgag tgacggtgtc agtcaaagag cgttgcatat gtgaaacgct
     6001 ctgcgccagg tcacccattt cactgcgccc gtcaatggtc agggtattcg ccaggttacc
     6061 accggcgatt tcgcaaatgt gagcaataat ttttgccagc ggagtaagca acatacggcg
     6121 aatgccgtac cacgccacca gcagaatcaa taccaccacc agcgcgataa ccgccagttg
     6181 ccactgggca aatcggtaat catctgcgtt gtcagtgacg atatcgcgat acagtttttc
     6241 actgctgagg gcgtactgag caaacgcttc gcccattgca ttttgcattc cctgggttgg
     6301 ctgagcgaaa taagctccag tattgccata atcaagataa tcaatcagtt cagttaacgc
     6361 tgtgtaatag tttttatatt tttcatcaaa attacggctg gtagcgacca tttcaggtaa
     6421 cggtgccatg cttttgaatt ttttataatg cgtcgcggcc tgggccaatg ttttcctggc
     6481 gctatcgagc aattcaactt tggcgttact ttgttgattg gaggaatcca tcatcatccg
     6541 caccgctgaa cgactcaggt taatgcgcgt ttgcagcatt aaatcccagg ttgacgtcag
     6601 ctcgccctgc tgttcccgta attgattgga aaccacaaag ctcttctggc tatggtgaag
     6661 ggaagaaaaa aacaaactgc cggaaataag ctgtaacagt gcgaataccc ccagcaccat
     6721 taccaacagc gtgactacgc ggatacggtt aatcataagg caccttcctg aaaacaagtt
     6781 gatctcgtta tcggcaagaa ggggggaaac tttattgctg atgccacccg ccgcgaaatt
     6841 gaaataaaaa acccgatgcg cagatcatcg ggttcatttc aattgaggaa atcgggagaa
     6901 ttacgccact tctgacgccg cgctatctaa cagcgccatc tcttcgctgt tcagcagttt
     6961 ttcgatgttc accagaatca acatacggtc gcccagtgcg cccagtccag tgagatattc
     7021 tgttgaaagc gtcacggcaa actctggtgc cggacgaatt tgctccgccg tcaatgaaag
     7081 cacgtctgaa acgccgtcaa ccacgatgcc gaccacccgc tgtccgagat tcaggacgat
     7141 aactaccgtg ttgtcgttat agtccacatc cacctggctg aacttaattc gtaagtcaac
     7201 aatcggcaca ataacgccgc gcagattcgt gacgcctttg ataaacgctg gcgtgttcgc
     7261 aatccgtgtt acctgatcgt agccacggat ctcctgcact ttcaggatat caataccgta
     7321 ctcttcatca ccaagggtaa ataccagaaa ttcctggcct gacggctcgc tggccagctt
     7381 tgttacattc gtcataccgg tcatattgtt acctttttac tcattcaggc ggcggtgttc
     7441 gccatacgtt gttctcggtt tatcgcctgc aaggcggaga catcaacaat cagtgccacg
     7501 ctgccgtcgc caagaatggt cgcagcagaa atgccgggga ctttgcgata gttactttca
     7561 aggtttttaa ccacaacctg gtgttgacca attaattgat ccaccagcaa ggcgtagcgg
     7621 cgaccgccac tttgtaagat caccacaatt ccctgggtgg cttcggtttt cgcgcccgcg
     7681 acgttgaaca ctttccacag ttcgacgatg ggcagatatt caccccgcac ttccagcacc
     7741 cgctcgccgc cggccagtgg atggagatcg gcttcacggg gttgcagtga ttccataaca
     7801 gcattcagcg gcagaatgaa aacttcatcc gcaacgcgta cggacatgcc gtcgaggatg
     7861 gccagcgtca gcggcagtaa aatgcggatc gtagtgccag taccctgctt cgactggatt
     7921 tcgacatgac cgcccatctc ctggatatta cgtttaacga cgtccatgcc gacgccgcgc
     7981 ccggagacgt cggtgacctg ctctgccgtg gagaagcctg gtgcaaatat cagcattgca
     8041 acttcgtcgt cgctcatgtt ttcgctgaca gtcaaacctt gcgaggccgc ttttgccaga
     8101 attcgctcac ggtttagccc cgccccatcg tcggtcactt caatgcaaat gttgccgccc
     8161 tgatgttcgg cagacagaat taaatttccg acgctgtttt tacctgcggc gagccgtttt
     8221 tctggcagtt caataccgtg atcgaggcta ttgcgtacca ggtgggtcag cgggtcgata
     8281 atgcgttcta tcaggctctt gtcgagctcg gtggaactgc ccaccagcgt cagttctacc
     8341 tgcttgccga gttttcccgc cagatcacgc accagccggg gatagcgact aaagacatat
     8401 tccatcggca tcatgcgaat cgacatcact gattcctgca aatcacgggc gttacgttgt
     8461 aactgcccca tgctggtaat caaatcacca tgattaaccg ggtccagttc gctggaacgc
     8521 tgggcaagca tggactgggt gataaccagc tcgccgacga ggttaattaa ttgatcaacc
     8581 ttttctaccg ctacacggat gctggtggat tcactgctgc gcgtcgtttt ttcccgctcc
     8641 acgcggccgg ttggcgcttg ttcggctgcc agtttaagca caggtggggt agctattttt
     8701 ggcgaaactt ctactgtttc aaaggtaatc tgatcggctt caatcacaaa acagagtacc
     8761 gctgtgatgt catcttcggc gatatcgccc ggtaatattg ccgagagaga atccgcccct
     8821 ttcaccacgt catttaacgt tgtcagatgc cccagctctt cttccagcag gtcgacttcg
     8881 ccggccttca ggcgcgaaag gataattcgt cgcggcaact gactgcgact ttgctcatct
     8941 tgcggttcac ttttggcaac cacacttaat cgggtcactg cggatggcgt ttcgcctttc
     9001 gcttctaatg ccagttgacg caaggcctgg cagatataat cgtagctggc ggcatccggc
     9061 tcttgcgact gtttataagc gtcgagctgt tcttgcatga tgtccttcgt ttccaaaaac
     9121 agattgataa tgtcggtgtt gagttgcatc tcacctcgtc tggcttcatc gagcaggttt
     9181 tccatcagat gcgtggtttc ctgcaaaacg ctgaagccaa aagttcctgc ccctcctttg
     9241 atcgagtggg cagcccgaaa gatggcattc aattgttcgg catctggcgc ttccggctgc
     9301 aaaaccagca gatgctgctc catatcagcc aacagttcgt ccgcttcatc aaaaaatgtc
     9361 tgataaaaat cgcttatatc gatgctcacg ctgtcacctc ggttcggctg atggcattgt
     9421 gggaacactg acctgtggtg caacctcagg tttttctagg gcgcttactg gctcattctg
     9481 gctttcggcg ttttcatgca aaatggcctg ttcggcttgt ttgttcagta ccaacaggct
     9541 gatgcgacgg ttgacggcat catcaggtcc gcgatcgctt aagcgcatcg ttgccgccat
     9601 gccgacgaca cgtaacactt tgccgctatc caaccctccg accatcagtt cgcggcggga
     9661 tgcattggcc cgatcggcag aaagctccca gttgctgtaa cctttctcac cgctggcgta
     9721 gggaaaatca tcggtatgac ctgaaaggct aatacggttg ggaataccgt tcagcacagg
     9781 cgcgatggcg cgcagaatgt cgcgcatata gggttcgaca tcggcactgc cggttctaaa
     9841 catcgggcga ttctggctat cgatgatctg aatacgtaga ccttcctgga ccagatcgat
     9901 tttgagatgg agacgtaacg cccgcagttt cggatcggac tctatcaact ggtcgagatc
     9961 accccgcaat ttccgcaatc gactttgctc catgcgtttt ttcagctctt cgatgttcgg
    10021 ctgcttattc acttccccct ggctttgggt gtaatcatca ccaccgccgg gaattgggct
    10081 ttcactattg gaaatgcgat cgccgcccgt aaccgctgtc gccagtggag tccggaagta
    10141 ctccgcaatc tgaatcaact cttttgggct ggagatggag atcagccaca tcaccagaaa
    10201 aaaggccatc atcgcagtca taaagtcggc ataagcaatc ttccacgatc catgtgctgc
    10261 cccgtggctt ttggctttgc gtcgtttgac gacaataatc ggatgcgctt gattcttcat
    10321 gcttcctcgg ttgtcgtctg ttgttgcgga tttttcaccg cacgcacatg ctcttccagt
    10381 tcaataaacg acggacgttc gctggaatag agcgttttgc gaccaaactc aacggcgata
    10441 ggcggtgcgt aaccgttcag attagaaagc agagtgactt tgacgcactg catcattttg
    10501 ctggtttcgg cgcttttctg acgtaaaaca ctcgctagtg gggaaataaa tccgtaagcc
    10561 aataaaatac cgaggaaagt ccccaccatc gcatgtgcga taagcgcccc cagctcggcg
    10621 gcaggacgat cggctgaacc taacgcgtga acgcccccca ttacagccgc gacaatacca
    10681 aacgccggaa gtgagtcccc gaccagcgcc agactgttcg ccgggacttc tgcctcgctt
    10741 tcgtgcgtct caatctcttc atccatcagg gcttcgattt cgaaggtgtt catgtgaccg
    10801 ctgataatca ggcgcagata atcgacgata aaatcaagca tgacgctatc cgcgaggatg
    10861 cgtgggtagc tggcgaagat ctcgctctca cggggatttt caatatcacg ttccagcgaa
    10921 aacatcccca tctgccgcga tttcgccatc aaccgataaa gcagagccag cagatccata
    10981 tacattgctt tggtgtattt ggagcgacga aacagcaacg gcagcgcttt cagcgtgcct
    11041 ttaatcgctt tgccattatt gccgacgata aacgacccaa tccctgcacc ggcaataatc
    11101 accagttcag cgggttgata gagtgctcca aggcttccac cggtcatcaa ataaccgccg
    11161 aaaactgtac cgagaacaac caggtaacct aataagataa gcacgacatc atccttccac
    11221 tgttgaccat gacaggatgt tcagtcgtca ggcgttaacg cgcgattggg gaaaaaaaaa
    11281 gcagcggtac gtcgttaccg ctgctggaat gttgcgccac accgtatcag ttaaacagcc
    11341 tgtactctct gttcatccag cagttgtggg ataatatcgg caggattctg ggaaagttta
    11401 cgtcttttta ctgcccggga tggcggttga cataagctgc aggcaaagct gccaacaggc
    11461 tggtgagcgt gggtaataaa attgccgccg cagcagttgc agctggaaag ttgcagtaat
    11521 ccactttcaa caaaccgcac caatgtccag gcacgggtta atgccagcag tggtccttct
    11581 tctgcttgtg ggcactgttc aaggtataaa cggtaggctt tgatcaccgc atcgacgcca
    11641 ttacacaaac cggttttcag taaaaactgc catgcattac agaacatcga agcatgaacg
    11701 ttttgttccc aggtcataaa ccagtcggtt gagaatggca gcatgccttt cggcggtggg
    11761 cttccgcgca gttctttata aagttttatc aggcgtccgc gacttaactg tgtttcgctt
    11821 tccagcatct gcaaacgagc gcccagggtg atcaattcca ttgccagctg aatatcccgc
    11881 gcttcctgaa caatgctttt ttcactcatg atcaggccct tttcttgcgc agcgcttctt
    11941 caggctgatt aacatcattc agcaagcgtg ttgagagcat gatgccggta tgaatttgct
    12001 ggagatcgtc aacgcgggaa tcttgcgtca actgagtaat cgtctggtgg ctgtcaaaac
    12061 ggaagtgaca aaccagctga ttggtttctg ccagcttaac catttgcgga ag
//
LOCUS       DAJTSA010000113        12062 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000113 DAJTSA010000000
VERSION     DAJTSA010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12062
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.113"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="OO369_003385"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO369_003385"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1558)
                     /gene="garK"
                     /locus_tag="OO369_003386"
     CDS             complement(413..1558)
                     /gene="garK"
                     /locus_tag="OO369_003386"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR5983537.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OO369_003387"
     CDS             complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OO369_003387"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR5983538.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OO369_003388"
                     /pseudo
     CDS             complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OO369_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OO369_003389"
     CDS             complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OO369_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR5983539.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5070..6641
                     /gene="garD"
                     /locus_tag="OO369_003390"
     CDS             5070..6641
                     /gene="garD"
                     /locus_tag="OO369_003390"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR5983540.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="OO369_003391"
     CDS             complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="OO369_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCR5983541.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7766..9046
                     /gene="kbaZ"
                     /locus_tag="OO369_003392"
     CDS             7766..9046
                     /gene="kbaZ"
                     /locus_tag="OO369_003392"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCR5983542.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            9069..9542
                     /gene="agaV"
                     /locus_tag="OO369_003393"
     CDS             9069..9542
                     /gene="agaV"
                     /locus_tag="OO369_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR5983543.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9553..10332
                     /locus_tag="OO369_003394"
     CDS             9553..10332
                     /locus_tag="OO369_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCR5983544.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            10322..11131
                     /locus_tag="OO369_003395"
     CDS             10322..11131
                     /locus_tag="OO369_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR5983545.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            11218..11652
                     /locus_tag="OO369_003396"
     CDS             11218..11652
                     /locus_tag="OO369_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5983546.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            11649..>12062
                     /locus_tag="OO369_003397"
     CDS             11649..>12062
                     /locus_tag="OO369_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5983547.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         2878 a         3252 c         3078 g         2854 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccaccc gtctcccccg aagaggacga cgacgaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcaatgccaa
      601 tcaccggttt atggtacttc ttcgccacgt ttgcgacgcc aatcggtacc ttgccgtgaa
      661 tactctggct gtcaatacgc ccttcaccgg tgatcaccag cgtacaatcg tgaatatgtt
      721 cctccagatt cagcgccgta gtgacgattt caataccact tttcagttcc gcaccaagaa
      781 acgccatcag cgccgcaccc ataccgcccg cagctcctgc gccggggaca tcttttacat
      841 caacatgcag cgctttttta atgacatcgg catagtgaga gaggttattg tccagctcaa
      901 caatcatcgc ttcactggct cccttttgtg ggccaaagat gcgcgatgcg ccgttatcgc
      961 ccaccagcgg attggtgaca tcacaagcga cgcgaatgac gcaatctttt aagcgcggat
     1021 cgaggccgga aatatcaata tcattcagag tattaagact accgccgcca aaaccaattt
     1081 cattgccgtt ggcgtcgcat aatttcgccc ccagcgcctg taccatgcct gcgccgccat
     1141 catttgtagc gctaccgcca atgccgataa taatgtttgt cgcgccgctc tccagcgcct
     1201 gcaggattaa ctcgcctgtg ccgcgtgaag tggtcacgag tggatcgcgt ttttccgcag
     1261 gtaccagctc cagcccactg gccgccgcca tttcaataaa cgcggttttg ccatcgccgg
     1321 agatccccca actggcattc actttctcgc ccagcggccc tgtaacccag gcgtgacgtt
     1381 cagaaccctg ggtggctgca atcatcgctt ccaccgttcc ttcgccaccg tcggcaaccg
     1441 gaatagaaac gtactgtgca tcaggaaaaa tttcccgaaa tcctttttct atcgcctgcg
     1501 caacctcgct ggcagataaa ctttctttat aagagtctgg ggcgattacg attttcatac
     1561 ctatgcctgt taccacatga cgccggaggg cgtttctctt attcggcctg gattccaggc
     1621 ccggattgca atacgccatc cgggcacgac gtcattaacg agtaacttcg actttcgcca
     1681 gtttttcgta gtagcacacc agggcgctat gatccgccgt tcctaaacca tctgctcgca
     1741 gtgcctgcat catctccata accgcagctg tgagcggcag ttgcgcgccg acgccgtggg
     1801 aagtatccag cgcatttgcc agatccttaa tatgcagatc aatacggaag cccggcttga
     1861 agttgcggtc catcaccatc ggcgctttgg catccagcac ggtactgccc gccagtccac
     1921 cgcgaattgc ctgatacacc aggtccgggt taacgcccgc tttagttgcc agcgttaacg
     1981 cttctgacat cgcggcaata ttcagcgcca caatgacctg attcgccagt ttggtgacgt
     2041 tacctgcacc aattgccccg gtatgcacca cggaacccgc cattgctttc atcaaatcat
     2101 agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac tgacagcgta ccgtcgatgg
     2161 ctttcggttc accgccgctc accggagcat ccagcatatc aatgcctttc gctttcagcg
     2221 cttcgctgat ttcacggctt gccagcggtg cgatagaact catatcgatc aataccgtac
     2281 ctggcttcgc gccttcaata atgccattct cacccagcgc cacctctttc acatgagggg
     2341 agtttggcag catggttatg atgacgtcgc actgttcagc gatcgcttta gccgtagagg
     2401 ctgtttctgc acctgcagca atcacgtcag caatagcttc tgggttacgg tcagcaacca
     2461 ccagcgagta acctgctttc agaaggtttt tactcattgg tttacccata atccccaggc
     2521 caataaaacc aactttcata gtcatatcaa tcatctctct tgttgcggtg gtggttattt
     2581 tttaaaggta tcagccagtt tatgagtggc agagcggaag acgccgagat cgctgccgac
     2641 agccacaaac gtcgcgcccc attccagata acgacgcgca tcggcttcga ccggcgcgag
     2701 gataccgctg ggtttgccgt gcgcgctggc acggttaaaa atgtgctgaa ttgctttttg
     2761 tacatccggg tgtgatgcat tgccgagatg gcctaatgcc gcggccagat cgctggggcc
     2821 gacgaagatg ccgtctacgc cttcggtagc ggcaatggca tcgacgttat ctacgccctg
     2881 ctgactttct atctggacca gaatagtgat gttcttgttc gactgagcga aataatccgc
     2941 cacggtgcca aacatattgg cgcggtgaga aacggagacg ccgcgaatgc cttccggtgg
     3001 gtaacgggtt gatgccaccg cctgctctgc ttcctctttt gtttctacaa aaggaatcag
     3061 gaagttatag aaaccgatat ccagaagacg cttaataatt tccggctcgt tggtcggcac
     3121 tcacactact ggcgcgctgg cgctgccttt caaggccatt aactgcggaa taaacgtgga
     3181 gatatcgttt ggcgcatgtt cgccatccag caccagccag tcaaacccag ccaaaccaag
     3241 aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa ccaatttgta cctgtttcgc
     3301 agccagtgcg gctttgaatt tattcgggaa aacatcgtta ttcatcgctt atacctttgc
     3361 ttatttctgc aattccatac gtttaatgtc gccaactacg aagaggtagc agaccatcgc
     3421 catcagcgct gaacatccca cgaaaaccag tgctgcattg aaggagtgca gttcacttac
     3481 caggtagcca atcaccagtg gagtgacaat ggaggcaaca ttgccaaaga cgttaaagac
     3541 gccgccacag aggccaacaa tctctttcgg cgcggtgtca gaaatcaccg gccagcccag
     3601 agcaccaaat cctttgccaa aaaaagccag cgccatcagc atgaccacca gcgtggtgtt
     3661 gttggtgtag ttacataaga tgatggtgga agccagcaac attcccagca caatcggtag
     3721 cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga taatccgaga agacacctcc
     3781 cagcacgccg cccgcaaaac cacacagtgc cggaatcgag gcgaccagac ccactttcag
     3841 aatcgacatg cctttttcct gcaccagata aatcgggaac caggtgagga agaaccaggt
     3901 gatggtgttg ataaaatatt gtccgaaaaa tacgcccaac atcatgcggt tagagagcaa
     3961 ttgcttgatg taatgcagtt tgggaccgct tgctgccgcg ctgcccggct ttttgtggtc
     4021 catatcgacc accgcgccat tttcagagat aaacttcagc tcttcctcag acatacgtgg
     4081 gtgatctgtc gggttatgaa tcaacttgat ccacagcgcc gtcagcacaa aacaaatcac
     4141 ccccataacg gtaaagacgt gctcccatcc ccaggcgaaa gtcagccagc caagcagagg
     4201 cgaaaagagc gccagcgaga aatattgtgc cgagttaaag atggcggagg cagtaccacg
     4261 ttctttcgtc gggaaccagg cggcgacaat acgggcgttc gccgggaagg atggagcttc
     4321 cgagaatccc agcataaagc gcataaagaa catggagatc cctgcccaag ccagcgggaa
     4381 catatcaaca aagccttgca ggaaggtgaa cagcgaccag aaaaagaggc tgtaggtgta
     4441 aacttttttc gagccaaact tatcaagcaa ccagccgccg gggatttgca tcagcaagta
     4501 ggcccagcca aaagcggaga agatgtaacc catcgaaacc gcacttaact gcaactcttt
     4561 tgccacttcg gtaccagcaa tagacagcgt tgcacgatcg gcgtagttaa cggcggtaac
     4621 aataaaaata atcagtaata ttaaatagcg ggtatgcaca cctttccttt tttcgtcaac
     4681 ggtgtccaga atcattttat ttacctcggg tacttatgct gatttttatt attatgggga
     4741 aggtgttatt tataagtttc atttatgccg taacgacaat gaactcggga attagtataa
     4801 gcagcgcgag aataataatc attgtgcaaa tgctaattta attaatactt tttaaatatt
     4861 attttgagca tatgcacata aggttgcgcg ctaaagcaca gatttgcgct ttaccttgcc
     4921 gggcggcact gcaatcccta aaatgattga cattgatcac atttctgcgt ttaaactcct
     4981 gacattctta tttcacccaa tgaaatcatt tatttttaaa tgagaccagg tcctcatttt
     5041 aataacccct ggctggagaa tattgcacaa tggccaacat cgaaatcaga caagaaacgc
     5101 caactgcgtt ttatataaaa gttcacgaca cagataatgt ggcaattatt gttaatgata
     5161 atggcctgaa agcaggaacg cgttttccgg atgggctgga attaattgaa catattcccc
     5221 aggggcataa agtcgcattg ctggacattc cggctaatgg tgaaattatt cgttatggcg
     5281 aagtgattgg ttacgccgtg cgtgcaatcc cacgcggaag ctggatcgac gaatcaatgg
     5341 ttgtactacc ggaagcgccg ccgttacaca cgctgccact ggcaaccaaa gtcccggaac
     5401 ccttaccgcc gctggaagga tacacctttg agggctatcg caatgccgat ggcagcgtgg
     5461 gcaccaaaaa cctgctcggc atcaccacca gcgtccactg tgtggcaggc gtggtggact
     5521 atgtagtaaa aatcattgaa cgcgatctgc taccgaaata cccgaacgtc gatggcgtgg
     5581 tggggctgaa tcatttgtac ggttgtggcg tggcgattaa cgcaccggca gcagttgtac
     5641 ctattcgtac cattcacaat atttcgctga accctaactt tggcggcgaa gtaatggtga
     5701 ttggcctggg ttgtgaaaag ttgcagcctg agcgcctgct gactggaacg gatgatgtgc
     5761 aagctattcc agtagaaagc gccagcattg tcagtttgca ggatgaaaag catgtcggtt
     5821 ttcagtccat ggtcgaggat attttgcagg tcgccgaacg ccatctacaa aaactgaatc
     5881 aacgtcagcg agaaacctgt ccggcttcag aactggttgt cggcatgcat tgcggtggca
     5941 gcgatgcgtt ttctggcgta acggcaaacc cggcggttgg ctatgcgtct gatctactgg
     6001 tgcgctgcgg cgcaacggtg atgttttcag aagtcacgga agtgcgtgac gcgatccatc
     6061 tgctgacacc acgcgcagtg aacgaagagg tcggcaaacg gctgctggag gagatggagt
     6121 ggtacgataa ctatctcaat atgggaaaaa ccgaccgcag cgccaaccct tcgccgggca
     6181 acaagaaagg cggtctggca aacgtggtag agaaagcgct cggctccatt gctaaatcgg
     6241 gcaaaagtgc aattgttgaa gtgctgtcgc ccggtcaacg cccgactaaa cgcggattaa
     6301 tttacgccgc gacgccagcc agcgattttg tctgtggcac gcaacaggtg gcttcgggta
     6361 tcaccgtgca agtgtttacg accggtcgtg gtacgccgta cggcctgatg gcggtacccg
     6421 tcattaaaat ggcaccccgc accgagctgg cgaaccgctg gtttgattta atggatatta
     6481 atgcgggcac catcgctacc ggcgaagaaa ctattgaaga ggagggctgg aagttgttcc
     6541 actttattct cgacgtcgcc agcgggaaga agaaaacctt ctcggatcaa tggggactgc
     6601 ataaccagct ggcggtgttt aacccagcac cggtgacctg ataattcaat tcgtattatg
     6661 cccgatagca cacgtttatc gggcatattg agtcacagcg aagacgcgtg atccccgacc
     6721 agaatcactt caaccccagc ctttcgcagt ccttccagac tatccgcagg aatgccttca
     6781 tcaacaatga tcatgtcgat acgttgagta tcaatgatct tatgtaaact ggaacgattg
     6841 aatttactgg aatcggtgac cacgatgatc cgttccgcaa cttcgcacat ccgacggctt
     6901 aaacgggctt catcttcatt atgtgtgctg acgccgcgct ccagatcgat cgcatctaca
     6961 ccaagaaaca gcatatcgaa gtggtaattt tgcagcgatt gctcagcctg atcgccgtaa
     7021 aaagattgcg actgacggcg caaatgcccg ccggtcatca gcagctcaac gccttccgct
     7081 tccagcaacg cattagccac gttcataccg ttggtcatcg caattacgtc agtgtacttg
     7141 cgcatcagac gagcaatctc aaaagtggtg gtcccggaat cgaggatcac ccgatgacct
     7201 ggctgaatca actcaacggc agctttcgca acgctgcgtt tcatcgcggt gttcagtgcg
     7261 cttttatctt ccactgatgg ctcgactgac ggcgtcgtgc tatcgcagat caacgcgcca
     7321 ccataggcac gcacagcgat cccctgcttt tccagaaacg ccagatcatt gcggatcgtc
     7381 acagtagata cgccatacaa tgccgacaga tcgttaacct gcacactccc ttgctgtcgc
     7441 agacgctgaa tgatctgttc tcgtcgctcg ctggtgcctg tcactcgctt ctcacctgaa
     7501 gcgtcggtat tactcatagt aagtcctttc gtaaaacttt cgtttcattt cgttttgcct
     7561 attaacgcct ttctattaag caaatgcaag cccaccttgc ccattggcgc aagctactct
     7621 cgtttcagtg actttcatta cgtttctttt gtgaatcaga tcagaaaact attatctttc
     7681 gttttatttt tatctcacca tgacgcagta tcaattgaaa caaaacgaaa gatcaatatc
     7741 gcagtaatct gaactggaga ggaaagtgaa acatctgaca gaaatggtga gacagcacaa
     7801 agcgggcaaa acaaatgcaa tttatgccgt ttgttccgca catccgctgg tgctggaggc
     7861 tgcaatccgc tacgccagtg caaaccaaac gccgttactg attgaagcaa cctccaatca
     7921 ggtagaccag ttcggcggtt ataccggaat gacgcccgcc gattttcgcg gctttgtttg
     7981 tcagctcgcc gactcgttga atttcccgca ggatgcgctg attctgggtg gtgaccatct
     8041 ggggccaaac cgctggcaaa acctgccagc cgctcaggca atggccaatg ccgatgattt
     8101 gattaaaagc tacgttgcgg caggattcaa aaaaatccac cttgattgca gcatgtcctg
     8161 tcaggacgat ccaattccct taactgatga catcgtggct gaacgcgccg cccgtctggc
     8221 gaaagtggcg gaagaaacct gtcgcgaaca ctttggcgaa gccgatctgg agtatgtcat
     8281 tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc ttaagcgagc tggcggtcac
     8341 cacgccggat gccgcccgcg ccacgctgga agcccatcgt cacgcctttg aaaagcaagg
     8401 tttgaatgcc atctggccac gcattattgc cctggtggtt caacccggcg tcgaattcga
     8461 tcacaccaac gttattgatt atcagcccgc caaagcgagc gccttaagcc agatggtcga
     8521 aaactacgaa acgctgattt tcgaagcgca ctctaccgat tatcaaacgc cgcaatctct
     8581 gcgccagctg gtgattgacc actttgccat tctgaaagtt ggcccggcgc tgaccttcgc
     8641 cctgcgtgaa gccctttttt ctctggcggc gattgaagaa gaactggtac cagcgaaagc
     8701 ctgttccggt ctgcgtcagg tgctggaaaa cgtgatgctc gaccgcccgg aatactggca
     8761 aagccactac cacggtgacg gcaacgcgcg tcgtctggcg cgtggttaca gctactcaga
     8821 tcgcgtgcgc tattactggc cggacagcca gattgatgac gctttcgctc atctggttcg
     8881 taatctggcg gattcaccaa ttccgctacc gctcatcagc cagtatctgc cgctgcagta
     8941 cgtgaaagtt cgctccggcg agctgcaacc gacgccacgg gaactcatta taaaccatat
     9001 tcaggacatc ctggcgcagt accacacagc ctgtgaaggc caataagcaa aacaaagagg
     9061 aacatgctat gccaaatatt gttttaagcc ggattgatga acgcttgatt cacggtcagg
     9121 tcggcgttca gtgggtcgga tttgcggggg caaatctggt gctggtagcc aacgatgagg
     9181 ttgccgaaga tccggtacaa caaaacctga tggaaatggt actggcagaa gggatcgccg
     9241 tacgtttctg gacgctgcaa aaagttatcg acaacattca tcgcgccgcc gatcgacaga
     9301 aaatcctgct ggtttgtaaa acacccgccg atttcctgac gctggtgaaa ggtggcgttc
     9361 cggtgaatcg cattaacgtt ggcaatatgc actacgccaa tggcaaacaa caaatcgcca
     9421 aaacggtttc tgtggatgcg ggcgatatcg cagcatttaa cgacctgaaa gccgctgggg
     9481 tggaatgctt cgttcagggc gtcccgacag agcctgctgt ggacctcttt aaattacttt
     9541 gagggattca tcatggaaat cagcctgttg caggcatttg cgttgggcat tatcgccttt
     9601 atcgctggcc tagatatgtt taacggctta acccatatgc accgcccggt ggtcctcggc
     9661 ccgttggtcg ggctggtact tggcgatctg cataccggaa ttttaaccgg cgatacgctg
     9721 gaactagtgt ggatggggct ggccccgctg gcgggcgcac agccgcctaa cgtgattatc
     9781 ggtactatcg tcggcacggc gtttgccatt actactggcg tgaaacccga tgtcgcggtc
     9841 ggtgtcgccg tacctttcgc tgtcgcagta cagatgggga ttaccttcct gttctcggtg
     9901 atgtctggcg tgatgtctcg ctgcgaccgg atggcggaga acgccgatac tcgcggcatt
     9961 gaacgcgtga actatctggc actgctggca ctcggcactt tctattttct ctgcgctttc
    10021 ctgcctatct acttcggcgc ggaacatgca aaaaccatca ttgatgtcct gccgcaacga
    10081 ttaatcgacg gcctcggcgt cgcgggcggc atcatgccag caatcggttt tgccgtgctg
    10141 ctgaaaatca tgatgaaaaa cgtctacata ccctacttca tcctgggctt tgttgccgca
    10201 acctggctca agttaccggt gctggctatt gctgccgccg cgctggcgat ggcgctgatc
    10261 gatctgctgc gtaaatctcc tgaaccgact caacctgcgg cacagaaaga ggaatttgaa
    10321 gatggcatct aatcaaacca ccctgccgaa cgtcagcgaa aatgaagaaa cactgctgac
    10381 tggcgtcaat gaaaacgtgt atgaagatcg gagcattgcc gcggagctga cgaaaaaaga
    10441 tatcaaccgc gtcgcctggc gttccatgct gttacaagcg tcttttaact acgaacgtat
    10501 gcaggcttct ggctggttgt acggtctgct gcctgctctg aaaaagatcc acactaataa
    10561 acgcgacctg gcgcgcgcca tgaaggggca tatgggtttc ttcaataccc atccgtttct
    10621 ggtgacgttt gttatcggca ttatccttgc gatggagcgt tctaagcagg acgttaacag
    10681 tattcagagc accaaaattg ccgtcggtgc gccgcttggc gggattggcg atgcgatgtt
    10741 ctggctaacg ctactgccga tttgtggcgg gataggcgca agcctcgcgc tacaaggctc
    10801 tattcttggc gcagtcgtct ttattgtgct gttcaacgtg gtacacttgg ggctgcgttt
    10861 tggtctggcg cattatgctt accgcatggg cgtggcggcg attcccctta ttaaagcgaa
    10921 taccaaaaaa gtcggccatg ctgcgtcaat cgttggaatg acggtaatcg gcgcactggt
    10981 ggcaacctat gttcgtttaa gcaccacgct ggaaatcacg gcgggcgacg cagtagttaa
    11041 gttacaggct gatgttatcg acaaactgat gcctgccttc ttaccgctgg tctacaccct
    11101 gaccatgttc tggctggtac gccgcggcta gagtccgctg cgcctgattg cggttaccgt
    11161 ggttctaggg atcgtcggta aattctgcca tttcctttaa atacaatgag gtattagatg
    11221 ttaagtatta ttttgacagg gcatggcggg tttgccagtg gcatggaaaa agcaatgaag
    11281 caaatcctcg gcgatcaatc gcagtttatt gccatcgatt ttcccgaaac ctcaagcgcc
    11341 gcgctgctta catcgcagct tgaagaggcc atcgcccaac tggattgtga agacggcatt
    11401 gtttttctga cggatttgct gggcggcacg ccgtttcgcg tagcttcaac gctggcgatg
    11461 caaaaaccgg gctgtgaagt gattaccggc accaatttgc aactgctact ggagatggtg
    11521 ctggagcgcg aagggttaag cggtgaagaa ttccgtgtac aggcgctgga gtgtggacac
    11581 cgagggctga ccagtctggt ggacgagtta ggccgctgcc atgaagaatg tccggtcgag
    11641 gaaggaatat gacacacgtt ctgcgcgcca gaaggctgct gactgaagag ggatggttcg
    11701 atgaccatca gttgcgtgtt gctgacggtg tcatcgcagc aatcgaaccg attccagtgg
    11761 gcgtgactga acgcgatgcg gaacggctct gccccgctta catcgacacc catgtacacg
    11821 gtggtgcggg cgttgatgta atggatgacg cgccggatgt actcgacaag ctggcaatgc
    11881 acaaggcacg cgaaggtgtc ggcagttggt taccgaccac cgtaaccgcg ccgcttaata
    11941 ccattcatgc ggcgctgaaa cgtattgctc aacgttgcca acgcggcgga cctggtgcgc
    12001 aagtgctggg gagttatctc gaaggaccgt acttcacgcc gcagaataaa ggcgcgcatc
    12061 cg
//
LOCUS       DAJTSA010000114        11924 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000114 DAJTSA010000000
VERSION     DAJTSA010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11924
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.114"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..2341)
                     /locus_tag="OO369_003398"
     CDS             complement(<1..2341)
                     /locus_tag="OO369_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5983548.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTEGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNVE"
     gene            complement(2275..2898)
                     /locus_tag="OO369_003399"
     CDS             complement(2275..2898)
                     /locus_tag="OO369_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCR5983549.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2895..4583)
                     /locus_tag="OO369_003400"
                     /pseudo
     CDS             complement(2895..4583)
                     /locus_tag="OO369_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="OO369_003401"
     CDS             complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="OO369_003401"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR5983550.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7506..8228
                     /locus_tag="OO369_003402"
     CDS             7506..8228
                     /locus_tag="OO369_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR5983551.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8356..10290)
                     /gene="yfaL"
                     /locus_tag="OO369_003403"
     CDS             complement(8356..10290)
                     /gene="yfaL"
                     /locus_tag="OO369_003403"
                     /inference="COORDINATES: protein motif:HMM:NF007285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCR5983552.1"
                     /translation="MMEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMA
                     DSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGD
                     GATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATD
                     GVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGT
                     LLFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQ
                     NNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDP
                     TPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHY
                     TAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADN
                     QNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLE
                     AGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTA
                     VGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAW
                     QKGSDDFAQTAGFLSMTVKW"
     gene            complement(10300..11895)
                     /locus_tag="OO369_003404"
     CDS             complement(10300..11895)
                     /locus_tag="OO369_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HCR5983553.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT         2756 a         3307 c         2945 g         2916 t
ORIGIN      
        1 attcgacatt gaccagttca ccaggctggt aatgggtttt gtccgttttc acccggatat
       61 caagctgagg aacggcaact ttgatcccgg cgttctgaaa actgtactga ccattacggg
      121 tatacagcac cgaaaaagtg atgttaggcg caaaggaatt gctcactggt acccgggctt
      181 catacagggt atcgtttaaa cgttgtagcg ttagccagtt tgccggatgc gaaagcagcg
      241 actgctgttc cacgcgatcg cgttccagcg tcaataacgc ttcatcaatt ggctccggga
      301 aagtaatcag catcttcgcg gtttcgcctg gctggtacag cgttttatcc gccacgatat
      361 ctaccgtacc agtatgcgcc gtgctgccct taccgctgac ggcatgactt aacccagcga
      421 gaattaagcc gtctttatcg cgtaatgtca gattgtagtt gccaggttta gcgaaattga
      481 cggtaaagga tttgccgcct gacggtagct ctccgctatg gctcgtgcgg tcttcgagac
      541 gcaaccattc atacgtaaca ggaacctgtt ttgaagattc cagcgcggca taacggaaca
      601 caaccgactc gccgctatta ctgtattgtg cggcggtact taaagagtaa tgcgccaggc
      661 cgcgttcaat gaggatctct ttggtggtgg tgacgcgata cgccgcgccg tcactggcgg
      721 agacggttaa caaatagcgg ctcggtttat cggcggcggg gagatttaac gccacatgac
      781 cgctggcgtc ggacaccgtt tcgctgcctt ccagcgacac ggggaaacgt ccggcataac
      841 gcaaatcgtt accgaccatt gataattgct gagcgcgcaa acttaactgc acgcgggcat
      901 tttttaccgg ctcgccatcc gggtagagca gttgcagttt gccgctgacc gcttcgccag
      961 ttttgaactc ttttttgtcg agagctaaac caatctcgaa atgtggcttg atgtagtttg
     1021 ccacgcgaaa actgctgcta tagacctgat tgcggtaagc aagacgtaac tcataacctc
     1081 cggcaacggc attttctggc aggcggaaac ttccctgccc gccattgcgc gcatccagcg
     1141 tgacatcgac ggtttgcaac agactgccgt tagcgtccag caccgaaagc ttcgccgggg
     1201 cgctgacgat gggggatgaa tgcaacggat cgcggaactc gcggcccatc actttaacat
     1261 cgacacgatc gcctgcgcga tatagcgggc gatcggtaaa aatatacaag cgggtgttgt
     1321 agatttcgct ttcgtagaag aagttctcgg agacaaaaac gccgccttca gcatccttac
     1381 ccagaatgta tgaacgttct ggcgatatat gttgtaactg caaagtaccg ctgtcatcgg
     1441 tcaccccgcg ggtcatcacg ccaagacctt cagtccacaa gatctctgag ccgggcttcg
     1501 cttcaccctg ttttttaccc gcggtccata ccagaagctc gttacctgac actttgctaa
     1561 gcgccacggt atcggaaaca aacaccaccg tcgtcgcccg atacccacct accatcgcct
     1621 cgacgaggta cagccccggc tcttgttggc cgagaggaat ataaatatta cccggctgcg
     1681 gcgagatgaa attgctggat gcgccttcca gttttacccc ttgctgcggc tcgaccggtt
     1741 tagcctgcca tagtggataa cggaactgtt ccaccaaggg atattttttc agcggggaaa
     1801 actggttgtt ctgtacataa cgggaaggtt taataatggc attgccgaga tgtaattcgg
     1861 gtaatgcctg agtcacattc tgccgtgact gagaagagaa agtacgctgc atcacgcggc
     1921 gagatttgcc gtaccagtta tcccacagcc aggtcagcgt attgttcagc ccgtcgccca
     1981 gatattgcgg ttgcaccaca atgcgatgca ggtttttctg ctggcgcaaa aatgccatcg
     2041 ggtcaggaat acgatatagg cgaacgtcca cgccgccgta ctcttccatc tgatagcgcc
     2101 gataatcacg ccccggcgct tccagtcgca ctttcgcctc ttcactgctg ctaaaactgc
     2161 tgtcagcaag caaaaagaat gttcccccgg cgggcggcgc atagttactg gaaggaagcg
     2221 aatcatcagc tttagcaagc cctgttccca caagagacag ggacaagcac gctgctatcg
     2281 cgccagaaaa tttaaacgat aaatgccaat gaaattggga ttggaatcgt tgggtatcca
     2341 tcgggtgtcc ttccatgtca taagttgttg cagactgact gcgcgcattc cgttgtcagt
     2401 tttcgtggcg cttccggtgt ggtagatgac gtaacgcccc atccagacca ttaagtgctg
     2461 ggcatcgccc tgatcgaaaa aaatcatatc gccaggcagc gcctggttta tgtcttggcc
     2521 aataaactgg ctgttgtact gaatcaaatt aatcgcggtc acgtaggggc cgtttttccc
     2581 gctcccctga ttccagtttt gcgccagttg acgctgttca ggtgttagcg tcatctctgg
     2641 cggcaaatac tggctagata aaccgttact ttttagccat ttactgtcgt gaactttcag
     2701 cgtctcgttc gccgcaaatc gcaccaggcc cgcacaatcc tgctgatacc agcgtggact
     2761 tggcccctgg cggagctgtt cttgtgcaat gcgtacaaac caggcgcgaa acagcctgga
     2821 ttgttcgaca ttcagcattt cgctatgggc aacaacacaa cacagccagc aaatcagcgc
     2881 cagcagcccg tgtctcatag cggctgccag gttatgggta gccactgcca ggcgacaccg
     2941 ggttccatct gggccagctt catgacataa cgcggttgtt gagataaagc gtccagcttc
     3001 ggcattaata aagtttgtgc ggcgttataa aaaaccggtt cgagattctt cggcagactg
     3061 gtcaacgttt cgttacgcag cagtttcgcc acgccttgtg gattgatata gagcggaacg
     3121 atgccatcag tgggtattac atccaccatt gccgggcggg ttttattcag tgtttgcagg
     3181 gcgttattaa ccagcgtgtc atcgagggag aaaagcagcg ttttgttttg catcgcctgc
     3241 gacacacgga aaaaataatc cgacattaat tgatcgggtt gcgccgcctg cgctttcgga
     3301 tactgaccgt atcgggaact cacttcgcga caccagattt gcgcttcgcc ctgctgagtc
     3361 tggcttaccg gcaaaacacc ttctggcgct ttgctttcgt gcgcaccaat attttgcgta
     3421 aacagtttcc ctggcaattg cgcctgttcg gcagtgccat caaactgacc gacaaacagc
     3481 ggggtttgca attttgagtc ttcataccag cacagccccg cggcaccgtc taacgcccca
     3541 ttcagcttgt cgttttcctg gctgatgtgc gaaagcatct cttcggcaat accgtgtgaa
     3601 tacggcaccg ccacacagaa gctggctccg gcaggcatac tgttccagac cggcgtaaaa
     3661 tcgaaactgg catctacgct ggcggattca tcatttaacg ccacaaaact gtgccagccg
     3721 tcgttaccca tttcgaagcg tacgccagca aaagaaggca ttaaccgctg gtagccaaaa
     3781 cccagcaacc tggcgctgac cacgatgcgc aggcgtactg gcgttttttc ggctgtacgc
     3841 tcttccaggc caaagctggc ttgccagcgt tttttaccgc tcaacaaatc acttgcgatc
     3901 gccgtggctt cggtatcctg ctgatcatct ttaaacaaca tatccgtact ggaaaacacc
     3961 agcatcttgt cctgatatgt cgcgaacatc agggcgttat tgccgttata gcgcaactga
     4021 taaacaggaa tagtttcact atttatcttg atgctactga tttccgtttt gcttaactag
     4081 ctgtcgctgg tagcggcaaa caacaatggc tccagcagtt tgcttaaacc actgcgctgg
     4141 atcagcacca tgtaatgtga aagatggcct tgtttatcgt gccatagcgc cgcctgcgcg
     4201 ggctgatcta agagtgacga aaagagctta tctttcagcg tgagatcgtg ttcatagaca
     4261 atgcggcgaa tgctgccttc aatgcccaga cgatcggcat gattctgata atagaaaacg
     4321 aaatcttcgc tcagaacatc gtggagaaac ggaatggtga ggagatcttt gggaagctgg
     4381 ctcagagagt cgctgtctag aaagaggtcc ggctcattga gatcgatttg cagattgttg
     4441 tgcaccacca gcggcgacaa cgttttttct ggcccactgc cagcatattg caacgcccag
     4501 acgccagcgg aaagcagtgc tattgcgcca aaacctacaa gaccatagaa ccgccagcct
     4561 ttcgcctttt tttcaccact cattgccaca ttccttgtgt atagccagcc attttttacg
     4621 ggcacagcca aactttaccg cgccctaata cgacaaaagc ccagactttg cagcctggac
     4681 ttttcaattc aaacaaggga gatagctccc ttttggcatg aagaagtaaa attattcttc
     4741 ttctggctcg tcgtcaacgt ccacttccgg agcaatttca tcatcccctt ccgcggcact
     4801 gccgtcgatg gtatccagat cttcctcgtc aaccggttca gcaacacgct gcagacccac
     4861 tacgttttca tcttccgcag tacggatgag gatcacgccc tgggtgttac ggcccacgat
     4921 gctgatttct gaaacgcgag tacgtaccag cgtaccggca tcggtgatca tcatgatctg
     4981 gtcgcagtca tctacctgca ccgcgccaac cactaaaccg ttacgttcgg taaccttgat
     5041 ggagataacc cctttcgtcg cacgcgactt ggttgggtat tccgccactg cagtacgttt
     5101 accgtaaccg ttttgcgttg cggtgaggat tgcgccatcg ccacgaggca cgatcagaga
     5161 gacgacttta tcgccttcac ctaagcgaat accgcgcaca ccagtggtat tgcagcccat
     5221 cgcacggaca gaagactctt taaagcgcac cactttacct tcggcggaga acagcattac
     5281 ttcatcttcg ccactggtca gatcaacgcc gatcagctca tcgccgtcaa ccagtttgat
     5341 cgccacttta ccggcggtac gcagacggtt gaattcggtg aggacggttt tcttcacagt
     5401 accgttagcg gtcgccatga agactttcac gccttcttca aactcggtca ccggcaggat
     5461 cgcggtgata cgttcgtcct gttccagcgg tagcaggttg acgatcggac gaccgcgcgc
     5521 gccacgagtg gcttccggca actggtaaac tttcatcgaa tagacgcgac cacggctgga
     5581 gaagcacaga atatggtcgt gcgtgttcgc caccagcagt cggtcgataa agtcttcttc
     5641 tttaatacgt gcggcagatt tacctttccc gccacgacgc tgcgcttcgt attcagaaag
     5701 cggctgatat ttaacgtagc cctggtgaga gagcgtcacg accacatctt cctgggtgat
     5761 cagatcttcc aggttgatgt ctgcgctgtt ggcggtgatt tcagtacgac gtttgtcacc
     5821 gaactgttca cgaaccagct ccagctcttc gcggatcact tccatcagac gatcggcgct
     5881 accaagaata cgcaacagtt ccgcgatctg atccagcagc tctttgtatt cgtcgagcag
     5941 tttttcgtgc tcaaggccgg tcagtttctg caaacgcaga tccagaatcg cctgagcttg
     6001 ctgttcggtc aggtagtaca gaccatcacg cacgccgaac tctggctcca gccattccgg
     6061 acgcgcagca tcgtcgccag cacgttccaa catggcggca acgttgccca gctgccacgg
     6121 attagcaacc agcgcagttt tcgcttctgc aggcgtcggc gcatgacgga tcagttcgat
     6181 gatcgggtca atgttcgcca gcgccacggc taatgcttca aggatatgag cacgatcgcg
     6241 agctttacgc agttcgaaaa tagtacgacg ggtcaccact tcacggcggt gacgaacaaa
     6301 cgccgcgatg atgtctttca ggttcatgat cttcggctga ccatggtgca atgccaccat
     6361 gttgataccg aaagaaacct gcaactgggt ctgggagtag aggttgttga gcacaacttc
     6421 accgaccgca tcgcgtttca cttcaatcac gatgcgcata ccgtctttgt cagactcgtc
     6481 acgcagcgcg ctgatgcctt ccacgcgttt ttcttttacc agttccgcaa tcttctcgat
     6541 caggcgcgct ttgtttacct gatacggaat ttcgtggacg ataatggttt cacgtccggt
     6601 tttggcgtca acttccactt cagcacgggc gcggatatat accttgccgc gaccggtacg
     6661 gtaagcttct tcaataccgc gacgaccgtt aatgattgcc gccgtcggga agtccggccc
     6721 cgggatgtgt tccatcagcc cttcaatgct gatgtcttca tcatcgatat acgccagaca
     6781 accgttgatg acttccgtca ggttgtgcgg cgggatgttg gttgccatac ctacggcgat
     6841 accggaagaa ccgttcacca gcaggttagg aattttggtt ggcatgacgt cgggaatttt
     6901 ttccgtgccg tcatagttat caacgaaatc gaccgtctct ttttcgagat cggccatcag
     6961 ttcatgggca attttcgcca gacggatttc cgtataacgc attgccgccg cagagtcgcc
     7021 gtcgatggaa ccgaagttac cctgaccgtc taccagcatg taacgcagcg agaatggctg
     7081 cgccatacgg acgatcgtgt cataaaccgc cgagtcacca tggggatggt atttaccgat
     7141 tacgtcacca acgacacggg cagatttttt ataggctttg ttccagtcat tgcctagtac
     7201 gttcatggcg taaagtacgc gacggtgtac cggcttcagg ccatctcgga catctggcag
     7261 cgcacggcca acaatgaccg acatcgcata atccagatag gagctcttta gctcttcctc
     7321 aatgttgacc ggtgtaattt ctctcgcaag gtcgctcatc taaccgctat ccctctactg
     7381 tatcccggat tcaaaggtcg caaattataa cacagccgcg cagtttgagg taaacctata
     7441 cgctttattc acatccaatg cctgatatac tcgtttgtct tgccaattac ggagtagaag
     7501 tgccaatgaa tgccgaaaaa tcgccggaaa accataacgt agaccacgaa gagatcgcta
     7561 aatttgaagc cgtcgcctcc cgctggtggg atcttgaagg tgagttcaaa ccgttgcacc
     7621 gcattaaccc gctgcgtctg ggctatattg ccgagcgtgc tggcggttta tttggcaaaa
     7681 aggtgctcga tgtcggttgt ggcggcggca ttctggccga gagtatggcg cgcgaaggcg
     7741 cgacggtgac cggtctggat atgggctttg agccattgca ggtggcaaag ctgcacgcgc
     7801 tggaaagcgg cattcaggtg gattacgtgc aggaaaccgt ggaagagcac gcggcaaaac
     7861 atgccgggca gtatgatgtg gtgacctgca tggagatgct ggagcacgta cccgatccgc
     7921 agtcagtggt cagagcctgt gcgcaactgg tgaaaccagg cggcgatgtc tttttctcga
     7981 cgcttaaccg caacggcaag tcatggctga tggcggtggt tggcgcggaa tatattttgc
     8041 gcatggtgcc caaaggtacg catgatgtga agaagtttat taaaccggca gagctgcttg
     8101 gctgggtgga tcagaccagt ttaaaagagc gacatataac cgggctacat tacaacccga
     8161 tcactaattc ctttaagctc ggccccggcg tggatgtgaa ctatatgctg cacacgcaga
     8221 ataagtgagg ttgatgtttg gccgcgccaa tgcctgatgc gacgcttgca gcgtcttatc
     8281 aggcctacaa atgctctccg taggccagat aaggcgttta cgccgcatcc ggcaattgtg
     8341 atggtcagac agcgattacc atttcaccgt catcgacaaa aaccctgccg tctgggcaaa
     8401 atcatcactc cctttctgcc acgccacgct gccgcgcagg gacactcgct gactgatatt
     8461 gcccgtgact cccactttta tttcaccccg tagcttcacc gcatcgtcac tgatagtgct
     8521 gccatcttct tcaatttccg tcgcatgggg atcgtgatag taattcagat ctaatgttgg
     8581 tgtgacacca accgcggtac gccattcgct gtgtaaaccc agccgcgtct gaatatcatc
     8641 cccctgcgat tgtgacacgc gcgcatggtt agcggcggta aaatcatcct gctgcacgcc
     8701 ctgataaatc acctgcgcct gcggttcaat caccacacca cgccccggta accactgata
     8761 cccagcttcc agcgaggcga taatccccga cgaatggtaa cgatccacgc catcttcatg
     8821 ttcggaaaca tcattgttaa accacgcgta ttgcagccag ctatccagcc aggccccctg
     8881 cttctgatta ccgtgctgat accagcttga ggtcagccca acggcataac cgtggttctg
     8941 gttatcggcg cgagttccgg tcatattcga gcggctgtcg ccctgattat cactgtagcc
     9001 accaaccgcc ccaagcatcc actcgccatc cgtgccccag cgcccgctaa acaagccgcc
     9061 gctaagctgc accgtagaag tgtcttcctg ctgagccagt tgccctgccg ccgtgtaatg
     9121 ataacgtccg ccgataacac gtaaattcag cgtctgacca tcgccaccag cgtgatcgcg
     9181 tcgctccatc ataaacgcct gatttgccgc ccgcaggtta ttgagataac cgccaacttt
     9241 ggcattcagc accggctggt aagcaggcgt aggggccggg tcgggggttg ggtcaggatc
     9301 cggcgtggga tcaggatcgg gagtcgggtc tggctcaggt ggcgatggcg gcgttacttc
     9361 ttgcgatcgc agataccagt cgttgttatc ttccactagc gtgtagtcat acgctcccat
     9421 gttgacgtag ccactgccag ccagactgaa ctgtgcattg ttttgaaact gcgtgggatc
     9481 agctgcgaaa tcaaccactt taatgcctgt cgatgtcggc tcaccaatcc ctgtaatgga
     9541 gttaaccacc acagttgtgt tgccagcagt attaccgttc atcaccaatt gatcgcttac
     9601 cgagtcatcg ccgtttaatt cgctgtcgaa tagtagcgta ccgccgccgg tataatcacc
     9661 gtttaccgtc agcgtatcgc cagcgacgcc attttgcagg ctaaccgcac cggacgtatt
     9721 ggtcagatca ccattaaccg tcacgtcagc aagataggtc aggctgtcag tttcaaggtt
     9781 ggtattaagc tcgcctgtca acgtcacgcc atcggtagca tttaccagcg tcccatcgcc
     9841 gttaaatagt gaggcattaa gggcgacgga agtatcctgc ccggtcagac gcaaaaccgt
     9901 accatcgctg atgtcgatag tgccgctgga agtagcatca attgactgaa tatcctgatc
     9961 cgcgccagta acgaacgttg ccccatcacc aacccacagt gacgaagcat aaggaaagat
    10021 atccgcaaca tcgcctttca gggtgccttc ttcgacacgt accgccgact gcgttgtacc
    10081 gctggcggtc agctccagcg tacccgcccc cgttttagta aatgtgctac cctcatcctg
    10141 atgctgcccg ctgctgtcag ccatcagtgc gccccactgc gtgtctaccc ccgcatcaac
    10201 tgccacttca ccgtcggcgc gcatttcaat atcacgccca tgctcagtcg tcgtagagcg
    10261 agtatcgaca ttggcagtaa tctccatcac gctttcttgt tacttatcgg taaagataac
    10321 ctggcggtta tagtgggtat ctccaagctg cgagttgtcg ctcaccatca gcgtaccgga
    10381 ggcgatttgt gttgtgccca aataactatt attgtttgcc agacttacct gcccgtcgct
    10441 cgcatcgacc accacgtaga cgccatcttt cccgtcgccg ccgatatgca aattgtcgcc
    10501 acctgcaaga tctaccacat cctgactgcc gataaccgtt ccgctgctgc cactgacttc
    10561 aaggccatca ttgtatgatg tgccgctctg ccaagtggag aaatcagaga gatcgagcac
    10621 accgccgttt aacacgatcg actgcggatc gtcctggaga gcggtaagat ctgccgcgtc
    10681 gccttccagc gaaagcaccg caccatcatc aacgactata tcgccggtta gcgccatcga
    10741 ctgcgcccct gccagcacgt agctgccgtt ttgcgcaatg gtgagctgac cagcaccttc
    10801 gatgatgcca gcaaaactgc cctgattaac agtaacgttg ccaccagcat cgatatttaa
    10861 agtgccattc tgaaagcccg tcaatgcgtg cacaaaagtt tgttgggtcg agccaacgtt
    10921 tagctcagcc tgattctgat actgatcaat actccctatc gtcagaccgt agcagtcttg
    10981 cggatcgtcc tggcaatgcg tatcgccgac attcatcagg gagttgctgc gacccagggt
    11041 aacttcaccg ttttcaatct gcatctcacc agtaaagtca ttgttatctg cattaagtac
    11101 cagatcgccg gaacctgttt tggtgattaa cccggtacca gcaatagagt caacagctcc
    11161 gtcattctct gtattgccga taaccagcgt ttttccgtcg gcaatatcaa aggtaacttc
    11221 acttaagccg agatacataa agccacccgc cgcagtggag ggaccatctc catagcctgc
    11281 tgcgctattg ttctcatcga ctaacacgcc gccgttctgg ctgtagctgt catcaacaga
    11341 aatatcaata agatagggag ccgtcgcgct attggtatat atcgccccgc cataaccttc
    11401 ggctgtgtta tttgtaaagg cggtgttatt tattatcgta taacctgaag gatgcttgct
    11461 gtcgctatta ttatcggtaa catcgattgc cccgccatcg ccatcactgt aacttgttga
    11521 tgtatatgcc tggttattgt taaaaacatc atcacttaaa tagacgtcat tattaatggt
    11581 ataaattgcg ccacctttgc catcattagc gatattattg atggcgttag tgacacgtaa
    11641 atcgacggca ccagtatcat tagtaccaga agaatagatt gcgccaccat agccgcctgc
    11701 gacgttaccg gaaaaaataa catcagtaag attcagcgtt gagttttctt tagcaaatat
    11761 ggccccgccg ttattatatt ctcctgtaac ggtgttattg gcaaacagag tcattccagt
    11821 ttcattttct ggtaatagtg aaaattccgc tccttgttgc aaaaatacgg ccccaccgct
    11881 ggcattattg gtcatatccg aaaaaaccag ccattgccca tcgg
//
LOCUS       DAJTSA010000115        11888 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000115 DAJTSA010000000
VERSION     DAJTSA010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11888
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.115"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..386)
                     /locus_tag="OO369_003405"
                     /pseudo
     CDS             complement(1..386)
                     /locus_tag="OO369_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            910..2838
                     /gene="thrS"
                     /locus_tag="OO369_003406"
     CDS             910..2838
                     /gene="thrS"
                     /locus_tag="OO369_003406"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR5983554.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2842..3384
                     /gene="infC"
                     /locus_tag="OO369_003407"
     CDS             2842..3384
                     /gene="infC"
                     /locus_tag="OO369_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR5983555.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3481..3678
                     /gene="rpmI"
                     /locus_tag="OO369_003408"
     CDS             3481..3678
                     /gene="rpmI"
                     /locus_tag="OO369_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR5983556.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3731..4087
                     /gene="rplT"
                     /locus_tag="OO369_003409"
     CDS             3731..4087
                     /gene="rplT"
                     /locus_tag="OO369_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR5983557.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4204..4328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4210..4254
                     /gene="pheM"
                     /locus_tag="OO369_003410"
     CDS             4210..4254
                     /gene="pheM"
                     /locus_tag="OO369_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR5983558.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4537..5520
                     /gene="pheS"
                     /locus_tag="OO369_003411"
     CDS             4537..5520
                     /gene="pheS"
                     /locus_tag="OO369_003411"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR5983559.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5535..7922
                     /gene="pheT"
                     /locus_tag="OO369_003412"
     CDS             5535..7922
                     /gene="pheT"
                     /locus_tag="OO369_003412"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR5983560.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7927..8226
                     /gene="ihfA"
                     /locus_tag="OO369_003413"
     CDS             7927..8226
                     /gene="ihfA"
                     /locus_tag="OO369_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR5983561.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8327..9307
                     /gene="btuC"
                     /locus_tag="OO369_003414"
     CDS             8327..9307
                     /gene="btuC"
                     /locus_tag="OO369_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR5983562.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9370..9921
                     /gene="btuE"
                     /locus_tag="OO369_003415"
     CDS             9370..9921
                     /gene="btuE"
                     /locus_tag="OO369_003415"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR5983563.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9921..10670
                     /gene="btuD"
                     /locus_tag="OO369_003416"
     CDS             9921..10670
                     /gene="btuD"
                     /locus_tag="OO369_003416"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR5983564.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10748..11212
                     /locus_tag="OO369_003417"
     CDS             10748..11212
                     /locus_tag="OO369_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5983565.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11459..>11888
                     /gene="ydiV"
                     /locus_tag="OO369_003418"
     CDS             11459..>11888
                     /gene="ydiV"
                     /locus_tag="OO369_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR5983566.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGN"
BASE COUNT         3039 a         2741 c         3150 g         2958 t
ORIGIN      
        1 attaccctgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
       61 cgtggtttct agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt
      121 ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt
      181 ttgtgatatt ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa
      241 cttaagtcag aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct
      301 gatgaaggtg ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt
      361 ctgataatga tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg
      421 aggatgatga ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat
      481 tgtgtcactt ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa
      541 tactaccgcc aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg
      601 ctcgcagtaa acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct
      661 ggtgtactca ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct
      721 cccttttttt agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca
      781 tttgtgtacc tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac
      841 tgttaataaa caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa
      901 ggatataaaa tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct
      961 gtaagcccca tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca
     1021 gggcgcgtta atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg
     1081 tcgatcatta ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac
     1141 ctgttagggc atgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt
     1201 attgacaacg gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc
     1261 gaagcactcg agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag
     1321 aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt
     1381 tccattcttg acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa
     1441 tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa
     1501 ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt
     1561 atttacggta cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa
     1621 gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag
     1681 gaagaagcgc cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg
     1741 gaagtgtttg ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc
     1801 atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg
     1861 ttcaccacat cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac
     1921 gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag
     1981 tttggtagct gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt
     2041 ggatttaccc aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt
     2101 aacggatgta tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc
     2161 gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct
     2221 gaggcggacc tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt
     2281 gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca
     2341 tggcagtgcg gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat
     2401 gtgggcgaag acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg
     2461 atggagcgtt tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt
     2521 gcgccagttc aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa
     2581 ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag
     2641 attggcttta aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt
     2701 gataaagagg tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga
     2761 agcatggacg taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt
     2821 aaacaattgg aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac
     2881 cgtatcaatg gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag
     2941 cttggtattg tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta
     3001 gtcgagatca gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc
     3061 ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt
     3121 aaggaaatta aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc
     3181 ctgattcgct ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt
     3241 gagatggcgc accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa
     3301 gaactggcag tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg
     3361 ctcgctccta agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc
     3421 ttcacaggca ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa
     3481 atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt
     3541 ggttttaagc acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt
     3601 aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg
     3661 tgcctgccgt acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag
     3721 gagagcacat atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat
     3781 tttgaaacaa gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca
     3841 ggctgttatc aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt
     3901 ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag
     3961 caaattcatc aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga
     4021 tatcgcagta ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct
     4081 ggcataagcc agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa
     4141 cattgacttt tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta
     4201 acgcaagcaa tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag
     4261 gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct
     4321 ttttttgtat gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc
     4381 cagtctggac acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga
     4441 gcactgcccg ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa
     4501 cgaaccagtg tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt
     4561 gccagtgcga aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc
     4621 gtcgaatatt tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg
     4681 ccgccagaag agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag
     4741 caggcgctga atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg
     4801 gcggaaacga ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg
     4861 gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca
     4921 accgggccgg aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac
     4981 cacccggcgc gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc
     5041 cagacctctg gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc
     5101 gcgcctggcc gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag
     5161 atggaaggtc tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac
     5221 gacttcctgc gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc
     5281 ccgtttaccg agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa
     5341 gtgctgggct gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa
     5401 gtttactctg gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc
     5461 gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa
     5521 ggcaggaata gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga
     5581 tcgatagcga tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag
     5641 aaccggttgc cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc
     5701 atccgaacgc tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg
     5761 acatcgtctg cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg
     5821 gtgctgttct gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg
     5881 aagggatgct gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg
     5941 aactgcctgc ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata
     6001 acaccatcga aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg
     6061 cgcgtgacgt tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg
     6121 gtgcgactat cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct
     6181 atcttggccg tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag
     6241 aaaaactgcg tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg
     6301 tactgctcga actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca
     6361 ttgtggtgcg gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga
     6421 agctgaatgc tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca
     6481 tctttggtgg cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg
     6541 ctttcttcag cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg
     6601 cgtctcaccg ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg
     6661 cgacccgtct gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca
     6721 acgaagcaac gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc
     6781 tgataggcca tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg
     6841 aagtgaccga aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg
     6901 agattgaaga agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg
     6961 atgagccggt acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca
     7021 agcgcgtgaa aacgctgctc aacgacaaag gctatcagga agtgatcacc tacagcttcg
     7081 ttgatccgaa agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc
     7141 cgatctctgt tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg
     7201 tggtgtacaa ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt
     7261 tcgtaccaga tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga
     7321 tttgcggtaa ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg
     7381 atttgaaagg cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt
     7441 tccgtgcaga agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag
     7501 gtgaacgtat tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta
     7561 acggtcgcac tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc
     7621 aggcgcgcga gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag
     7681 aaaacgttcc cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag
     7741 ttggcgtaaa cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc
     7801 tcgccataag cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg
     7861 ctaccgtcgc caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt
     7921 gaacctatgg cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt
     7981 agcaagcggg atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg
     8041 gaaaacggcg aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat
     8101 caacgcccgg gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg
     8161 gtgaccttca gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac
     8221 gagtaatctg atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag
     8281 agtcaccgta aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc
     8341 ccgccaacaa cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct
     8401 ggcgcttctc ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac
     8461 tcctcgtggc gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct
     8521 ggttggtgcg gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct
     8581 ggcagaacct ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt
     8641 attgcttggg caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc
     8701 gcttatcatc actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt
     8761 attgctggct ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta
     8821 cttttccacc tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg
     8881 cgtagactgg cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg
     8941 ttgtcagtcc aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg
     9001 tttacccatg tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt
     9061 cagtgtggcg ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg
     9121 gttgtgtggt ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag
     9181 cgcattgctg ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat
     9241 tggcgtggtc accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg
     9301 acgttagccg caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta
     9361 ggagaaacga tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg
     9421 accacgctgg agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt
     9481 ggcttaacgc cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt
     9541 tttgtggtgc tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa
     9601 gagattaaaa cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt
     9661 gaagttaatg gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc
     9721 acagtcgcgc cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg
     9781 ctgtacccgg atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa
     9841 gtcatccagc gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt
     9901 aaactggcgt tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta
     9961 cccgcctggg gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc
    10021 cgaatggcgc gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg
    10081 gaagcattca gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc
    10141 atcgcgccta tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc
    10201 tgacactgca tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc
    10261 tggctcttga tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac
    10321 gcgtacgtct tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat
    10381 tgctgcttct tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca
    10441 aaattctgag cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca
    10501 accacacgtt gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca
    10561 gtggacgcag agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt
    10621 ttcgccgtct ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt
    10681 atcccgaaaa tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc
    10741 gtacggaatg cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca
    10801 ccataaagca ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt
    10861 gaacgaccag ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg
    10921 cggtgtggac tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct
    10981 gccccgtgaa accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct
    11041 gcctggtgac ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat
    11101 ttacgatacc aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc
    11161 acttgataat gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga
    11221 aaaattgacg gcgatgatac atcgccgtca atttactcct gaatacagta gtattttgca
    11281 gacaaaaatt ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggcccc
    11341 ctgaaaaagt ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt
    11401 atggcctcat cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat
    11461 gaagattttt ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa
    11521 ccagcaactc cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggcacggt
    11581 tcgtatcccg acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt
    11641 ttcagagcag ttggaattac taaaatcatg ccaacacttt tttattcaac ataaactctt
    11701 tgcctggctt aatttaacgc cacaagtggc tacattatta ttagacaggg ataattacgc
    11761 aggcgaacta ttaaaatatc cgtttattga gctgttgatt aatgagaatt acccgcatct
    11821 caatgaagga aaagataatc gcgatctttt gtccttatcg caaatgtatc cccttgtgtt
    11881 aggcaatt
//
LOCUS       DAJTSA010000116        11682 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000116 DAJTSA010000000
VERSION     DAJTSA010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.116"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..346
                     /locus_tag="OO369_003419"
     CDS             <1..346
                     /locus_tag="OO369_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5983567.1"
                     /translation="GNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQ
                     QQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQN
                     DLLLRATGGVCV"
     gene            complement(463..738)
                     /gene="yjfN"
                     /locus_tag="OO369_003420"
     CDS             complement(463..738)
                     /gene="yjfN"
                     /locus_tag="OO369_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR5983568.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(887..1216)
                     /gene="bsmA"
                     /locus_tag="OO369_003421"
     CDS             complement(887..1216)
                     /gene="bsmA"
                     /locus_tag="OO369_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR5983569.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            1398..2147
                     /gene="yjfP"
                     /locus_tag="OO369_003422"
     CDS             1398..2147
                     /gene="yjfP"
                     /locus_tag="OO369_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5983570.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(2144..2899)
                     /gene="ulaR"
                     /locus_tag="OO369_003423"
     CDS             complement(2144..2899)
                     /gene="ulaR"
                     /locus_tag="OO369_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR5983571.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(3007..4071)
                     /gene="ulaG"
                     /locus_tag="OO369_003424"
     CDS             complement(3007..4071)
                     /gene="ulaG"
                     /locus_tag="OO369_003424"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR5983572.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRMLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            4426..5823
                     /gene="ulaA"
                     /locus_tag="OO369_003425"
     CDS             4426..5823
                     /gene="ulaA"
                     /locus_tag="OO369_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR5983573.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            5839..6144
                     /gene="ulaB"
                     /locus_tag="OO369_003426"
     CDS             5839..6144
                     /gene="ulaB"
                     /locus_tag="OO369_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR5983574.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            6154..6618
                     /gene="ulaC"
                     /locus_tag="OO369_003427"
     CDS             6154..6618
                     /gene="ulaC"
                     /locus_tag="OO369_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR5983575.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            6632..7282
                     /gene="ulaD"
                     /locus_tag="OO369_003428"
     CDS             6632..7282
                     /gene="ulaD"
                     /locus_tag="OO369_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR5983576.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGAPDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            7292..8146
                     /gene="ulaE"
                     /locus_tag="OO369_003429"
     CDS             7292..8146
                     /gene="ulaE"
                     /locus_tag="OO369_003429"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR5983577.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            8146..8832
                     /gene="ulaF"
                     /locus_tag="OO369_003430"
     CDS             8146..8832
                     /gene="ulaF"
                     /locus_tag="OO369_003430"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR5983578.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(8961..9236)
                     /locus_tag="OO369_003431"
     CDS             complement(8961..9236)
                     /locus_tag="OO369_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5983579.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            9563..9958
                     /gene="rpsF"
                     /locus_tag="OO369_003432"
     CDS             9563..9958
                     /gene="rpsF"
                     /locus_tag="OO369_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR5983580.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            9965..10279
                     /gene="priB"
                     /locus_tag="OO369_003433"
     CDS             9965..10279
                     /gene="priB"
                     /locus_tag="OO369_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR5983581.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            10284..10511
                     /gene="rpsR"
                     /locus_tag="OO369_003434"
     CDS             10284..10511
                     /gene="rpsR"
                     /locus_tag="OO369_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR5983582.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            10553..11002
                     /gene="rplI"
                     /locus_tag="OO369_003435"
     CDS             10553..11002
                     /gene="rplI"
                     /locus_tag="OO369_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR5983583.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(11073..11636)
                     /locus_tag="OO369_003436"
                     /pseudo
     CDS             complement(11073..11636)
                     /locus_tag="OO369_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
BASE COUNT         2767 a         2872 c         3087 g         2956 t
ORIGIN      
        1 cggcaatatt atgtgcctgg atgtgttgcg cgttctcaat aagcaagcgg gcgtatacga
       61 cttattgtcg gaagcatttg tggaagtgaa agggcaggat cgctattttg atcgcgcggt
      121 tcgtcgttta cagcagcagc tgcgtaagcc agctgaagaa ctggggcgag agattactca
      181 tcagctattc ctgctgggct gcggtgcgca aatgttgaaa tatgcttctc cgccaatggc
      241 gcaggcgtgg tgtcaggtga tgttagatac gcgcggcggc gtacggttgt cagagcagat
      301 ccagaatgat ttattgctgc gggcgacggg gggagtgtgt gtgtaagcgt atacgactga
      361 tgcgacgctg gtttcgatta actaaatgaa atatgtgaaa attgtaggcc ggacaaggcg
      421 ctcgcgccgc atccggcatt gttcatttgc tgtagatgac actcatgcat acagtatcgc
      481 ctgtacgcgc cagttatcag gctgctggtc ttcgtacatg gtaataatgc ggtaccatga
      541 cgcaccttgt tcatcggcct ttaacgccac gatacgttcc acgtcctgcg gatcccctga
      601 aatattgcta actgagatct gacctatttc attcaggccc gtcacgcagt cagcactggc
      661 gaattctgca gactgtgccg tggcgctcgc aggataagtc actaattgca gcgagggtga
      721 ggcaagtaat tgtttcatcg tcagctccat ttttcgtttc cccacatgtt gcagggcatt
      781 ccatcgcggt aataaacgcc caagcttcca gccggtaatt tattgtgcac aggtaaacgc
      841 atagggacgc aaagcaatgt aaagctgact atgtaagttg tctgatttat ttacggtaca
      901 aaatggcttg tgaataccac tgtcctgtca cgatggtttc gtccaccatg acgacaacgt
      961 aataatcagc ttttgcagcg acagcttttg ctctgatttc tgctaatgcg tcatccggag
     1021 aaccccgaac catcgtgctt acgctaccta ttcgctgtaa cccttgcgtc tggtcgcggc
     1081 gaatctcttg cggatggtcc gttactggcg gtgctggctg tggcgtacct tgcagtgcgc
     1141 tacaggcact taacatcaac accaataata aactggcaaa ccggtaaata acgctattac
     1201 gtttcctgct aaccatagtg tagtgcctta ttaatttaac tttgggggaa tgttcccgaa
     1261 ttgttacctg gtacgcgatt ttcgaatgaa aacgtgacga aagcgtaacc cacatctcaa
     1321 cattatgaac taataccgcc agcagaaact tgtgctaagg atttcttcat gtcataccag
     1381 acaaaaagga gagacagatg attgaaatag aatcacgcga gctggcagat attcccgttc
     1441 ttcatgctta tcctgtcggg caaaaagata ccccgttacc gtgcgtaatt ttttatcacg
     1501 gctttacttc atccagcctg gtgtatagct attttgccgt tgcgctggcg caggctggtt
     1561 tgcgggtgat catgccggat gcgcccgatc acggtagccg ttttagtggc gacgaagcgc
     1621 ggcggttaaa tcaattctgg caaatcttgc tacaaagtat gcaggaattc actactttac
     1681 gtgcggcaat agccgaagaa aactggctgc ttgatgaccg tctggcagtc ggtggcgcgt
     1741 cgatgggcgc gatgacggca ctgggaatta ccgctcgcca ccccacggtg aaatgtaccg
     1801 ccagcatgat gggatcgggc tattttacat cgctcgcccg ttcactgttt ccaccgctga
     1861 tacctgaaac ggcagcacag cagaatgaat tcaataaaat tgtcgcgccg ctggcagagt
     1921 gggaagcgac aaaccacctg gaacaacttg gggacagacc tctactgctg tggcatggcc
     1981 tcgacgatga cgttgtgcct gccgacgaat cactacgttt gcagcaggcc ttaagcgaga
     2041 cgggacggga taaactgcta acctgttcat ggcagccagg cgtgcgtcac cgcattacgc
     2101 ctgaggcgtt agatgctgcc gtgacatttt tccgccagca tctttaaaca cgcagaatgc
     2161 tgaccccttg cgcttccagt tgttgcagga tttccgggtt agcatttttg ccggtgatca
     2221 gcatatcgat ttgatcggca cggctaaaaa gcattcccgc gcgttcgcca atcttactgc
     2281 tatcaaccag taccaccagt ttccctacca cgctcagcat cttctgctct gccattgctg
     2341 tcagcatatc ggttttatac aacccttctg cggtcagccc ttttccgctg gtaaacatcc
     2401 agtgcccggc atagagactg ttttcgctgc cctgcgggct taaagtgatg gactgacttt
     2461 tgttgtactg tccacccata atgatcacgc tgtcatgttc ttgatcgatc aggtaatttg
     2521 ccagcggtag ataattagtg atgatttgca ctggcttgcc acacatttcc cgcccaagca
     2581 gaaacgcggt ggagccgcag ttgatgacta cgctttcgcc cggattaacc agctgcgagg
     2641 ccgctttagc aatacgtact ttttcatcgt gattctgcgc ctgatgcaga ttcatcggcg
     2701 tccagcgcgg gcgctgttgg gtaatagctt ctgcgccatt gcgcaccttt ttcagtttgc
     2761 cgctttcgcc aagtttattg atatcgcgtc gcgcagtggc aggcgaaatt cccagacgct
     2821 caacgacttt ctcaacggtc acaaagccca attgtgcgag catttccagg aggatttgat
     2881 gtctttgtgc ttcagtcatg agctattccg ataaaatttg atttttttag atgatatttg
     2941 aaatagccag gaaatactac gccggatagc gcgaacttct ccacgctatc cggtataaac
     3001 gacagattac aggaatgact tgaacggcag atccggttca atagtgaagc aatcatcgaa
     3061 accgcgcgga tagtggtact cgaagttgtc tttatccagc ggccaggtaa atttgccgcc
     3121 aacctgccag ataaacggct tgaagccata cttcagacga tcttttttga tctcccacag
     3181 catgcggatc tcttgcggat cggcctggaa gtttgaccag atgtcgtggt ggaacgggat
     3241 aaccactttc gcattcaggg cttcacccat acgcagcata tcggcgctgg tcattttgtc
     3301 ggtaataccg cgcgggttct caccatacga tcctaacgcc acatcgatct gatgttcgtt
     3361 accgtgcttc gcataatagt tagagtagtg ggaatcgcca ctgtgatata gggaaccgcc
     3421 aggggttttg aacaggtagt ttactgcgcg atcgtccatg ccatctggca gtacgccagc
     3481 cgctttttga tcggcaggca gggtgatcag tgcagtacgg tcgaaagcgt caagcgcatg
     3541 aatttcaatg tctttcactt ttacaacatc gcccggtttg accacgatgc aacgctcttt
     3601 cggtacgccc cagccaatcc acaaatcaac gcaggttttc ggtccaatga atggtacgtc
     3661 atcagcacag ttctgcatca cggcagcagc gacgttaacg tcgatatgat cgttgtgatc
     3721 gtgagtcgcc agtaccgcgt cgatctggcg aatcgcaaac ggatcaagaa caaacggggt
     3781 ggtgcgtagg tttggctgca gttttttcac gccagccatg cgctgcatct ggtgaccctg
     3841 tttcattaac gggttaccgt gactttgttt gccagtgccg caccagaaat caacgcaaac
     3901 gttggtgcca ccttctgatt tcaaccagat cccggtgcag cctagccacc acatcgcaaa
     3961 tgtgccaggg gcgacctgct cttgttcaat ttcttcattc agccagctac cccactccgg
     4021 gaaagtgctc aggatccagg attctcgggt gatacttttc actttactca tcgccattta
     4081 ccttcatgat agttcaattc gaatcaacat gtgattggtt ttgattaatc ctgacactat
     4141 tccttcagga aggtaatgac cattttttga ctttttgcca gggaagttgt tgttgatttt
     4201 gagtggggaa agatttaatg gaatgtgtag ttaaattaac taaatgaatt taaataaata
     4261 attgtttcgt tgtgtgaatc ccactctatc catgtggaat tatttgcggg tcgcgtcaca
     4321 tttaatcata aataatcttg ttgtgattac ttttgaaaat tagagtgagt gcacaacatt
     4381 ccgggtgtgc ggaatacccg gttacctctt cttcaggaga tcgttatgga gatcctctac
     4441 aacatcttta ccgtgttttt taaccaggtc atgaccaatg ccccgttgtt gctgggtatt
     4501 gtgacctgtc tgggctacat cctactgcgc aaaagtgtca gcgttattat taaaggcacg
     4561 attaaaacca taattggttt catgttgttg caggcagggt ccggcatcct caccagcacc
     4621 ttcaaaccgg tggtggcgaa aatgtccgaa gtctacggca ttaacggcgc aatttccgat
     4681 acttacgcgt cgatgatggc aaccatcgac cgcatgggcg atgcctatag ctgggtaggt
     4741 tacgcggtgt tgttagcgct ggcgctgaac atctgttacg tgctgctgcg tcgcattacc
     4801 ggtattcgta ccatcatgct gactggtcat atcatgttcc agcaggcggg gctgattgcc
     4861 gttacgttgt ttattttcgg ctactccatg tggacaacca ttatctgcac ggcgattctg
     4921 gtttcgctct actggggcat tacctccaac atgatgtaca agccgactca ggaagtgacg
     4981 gacggctgcg gtttctccat cggtcaccag caacagtttg catcattaat tgcctataaa
     5041 gttgcgccgt tcctcggcaa aaaagaggaa agcgttgaag acctcaaact gccaggctgg
     5101 ctgaatattt tccacgacaa catcgtctcc acggcgattg tcatgaccat cttctttggt
     5161 gccattctgc tctccttcgg tatcgacacc gtgcaggcga tggcaggcaa agtgcactgg
     5221 acggtctata tcctgcaaac cggtttttcc ttcgcggtgg cgatcttcat catcactcag
     5281 ggtgtgcgca tgtttgtggc ggaactctct gaagcgttta acggtatctc ccagcgcctg
     5341 atccctggtg cggttctggc gattgactgt gcggctattt atagcttcgc tccgaacgcc
     5401 gtggtctggg gctttatgtg gggcaccatc ggtcagctga ttgcggttgg catcctggtc
     5461 gcctgcggtt cctcgatcct gattatccca ggctttatcc cgatgttctt ctccaacgcc
     5521 accatcggcg tgttcgctaa ccacttcggc ggctggcgtg cggcgctgaa gatttgtctg
     5581 gtgatgggga tgatcgaaat ctttggttgc gtctgggtgg tgaaacttac cggtatgagt
     5641 gcctggatgg ggatggcgga ctggtcgatt ctggcaccgc cgatgatgca gggcttcttc
     5701 tccatcggta tcgcctttat ggccgtcatc attgtaattg cactggctta tatgttcttc
     5761 gctggccgcg cactgcgcgc agaagaagat gcagaaaaac aattggcaga acagtctgct
     5821 taataaggag ttttgattat gaccgtacgt attctggctg tgtgtggcaa cggacaaggc
     5881 agttccatga tcatgaagat gaaagtggac cagtttttaa cccaatcaaa cattgaccac
     5941 acggtaaata gctgcgcggt tggcgaatac aaaagcgagt tgagcggcgc ggatatcatc
     6001 atcgcttcta cgcacattgc gggcgaaatc accgtgaccg gcaacaaata cgtggttggc
     6061 gtgcgcaaca tgctctctcc tgccgacttt ggcccgaaac tgctggaagt gatcaaagcg
     6121 catttcccgc aggatgtgaa gtaaggacgc gccatgaaat tacatgattc gctggcggag
     6181 aataaatcca tccgcctgca ggctgaagct gagacatggc aggacgcagt aaaaatcggc
     6241 gttgacctgc tggtggcggc ggatgttgtc gagccgcgtt actaccaggc gattctggat
     6301 gcggttgaac agcacggtcc gtactttgtg ctcgcaccag gcctggcaat gccgcacggt
     6361 cgcccggaag agggcgttaa gaaaaccggt tttgcgctgg tgacgctgaa aaagccgctg
     6421 gagttcaacc acgaagataa cgacccggtg gatatcctca tcaccatggc ggcggtcgat
     6481 gccaacacgc accaggaagt gggcatcatg cagatcgtca acctgtttga agatgaagaa
     6541 aatttcgacc gtttacgtgc ctgccgtacc gagcaggaag tactggatct cattgaccgc
     6601 accaacgcgg cagcttaaga aggaattgaa gatgtcatta ccgatgttgc aagtcgcgct
     6661 ggacaaccag actatggata gcgcctacga aaccactcgc ctgattgccg aagaagtcga
     6721 cattatcgaa gtgggtacca ttctgtgcgt gggcgaaggc gtgcgtgcgg ttcgcgatct
     6781 gaaagcgctc tacccgcaca aaatcgtgct ggcagacgcc aaaattgccg atgcgggcaa
     6841 aattctttct cgtatgtgct tcgaagccaa cgctgactgg gtgaccgtga tttgttgtgc
     6901 ggatatcaac accgccaaag gtgcgccgga cgtagcaaaa gagtttaacg gcgacgtgca
     6961 gatcgaactg accggttact ggacctggga acaggcgcaa cagtggcgcg acgcaggtat
     7021 tcagcaggtg gtttatcacc gcagccgtga cgcgcaggcc gctggcgtgg cgtggggtga
     7081 agccgatatc actgcgatca aacgtctttc cgatatgggc ttcaaagtca ccgtcaccgg
     7141 tggcctggcg ctggaagatc tgccgctgtt caaaggcatt ccaattcatg tgtttatcgc
     7201 cggtcgcagc atccgtgatg ccgcttctcc ggttgaagcc gcgcgtcagt tcaaacgttc
     7261 catcgccgaa ctgtggggct aaggagcgga tatgttgtcc aaacaaatcc cgcttggcat
     7321 ctatgaaaaa gcgctccccg ccggggagtg ctggctggaa cgcctgcgac tggcaaaaac
     7381 gttgggcttc gattttgtcg agatgtcggt agatgaaact aacgagcgcc tgtcgcgcct
     7441 cgactggagc cgcgaacagc gtctggcgct ggtgaatgct attgttgaaa ccggcgtgcg
     7501 tgtgccgtcc atgtgccttt ctgcccatcg tcgtttcccg ttgggcagcg aagatgacgc
     7561 ggtgcgggcg caggggctgg agattatgcg taaagccatc cagttcgccc aggacgttgg
     7621 cattcgcgtg atccagctgg cgggctatga cgtttactat caggaagcca ataacgaaac
     7681 gcgtcgtcgt ttccgtgacg gcctgaaaga gagcgttgag atggcgagcc gcgcgcaggt
     7741 gacgctggcg atggagatca tggattatcc gttgatgaac tccatcagca aggcgctggg
     7801 ttacgcgcac tatctcaaca acccgtggtt ccagctctac ccggatatcg gcaacctgtc
     7861 ggcgtgggac aacgatgtgc agatggagtt gcaggccgga atcgggcata tcgtcgcggt
     7921 gcatgtgaaa gacaccaaac cgggcgtctt caaaaacgtg ccatttggcg aaggtgtggt
     7981 ggatttcgaa cgttgcttcg aaacgctcaa acagagtggc tattgcgggc cgtacctgat
     8041 tgagatgtgg agcgaaacgg cggaagaccc ggcggcagaa gtggtgaaag cgtgtgattg
     8101 ggtgaaagcg cgcatggcca aagcaggcat ggtggaggcg gcataatgca aaagctaaaa
     8161 cagcaggtgt ttgaagccaa catggatctg ccgcgctatg ggctggtgac ctttacttgg
     8221 ggcaacgtca gcgctatcga ccgcgaacgc gggctggtgg tgatcaagcc cagcggcgtt
     8281 gcctacgaaa ccatgaaagc agacgatatg gtggtggtcg atatgagcgg caaggtggtg
     8341 gaaggggagt atcgcccatc ttccgacact gcgacgcatc tcgaactcta ccgtcgttac
     8401 ccgtcgcttg gcggcattgt ccatacccac tccactcatg ccaccgcatg ggcgcaggcg
     8461 gggctggcga tcccggcatt aggcaccacg cacgctgact acttctttgg tgatatcccg
     8521 tgtacgcgtg gattaagcga agaagaggtg cagggtgagt atgaactgaa cactggcaaa
     8581 gtgattatcg aaacgctggg caacgccgag ccgctgcata cgccgggaat tgtggtgtat
     8641 cagcacggac cgttcgcctg ggggaaagat gcccacgacg cggtgcataa cgcggtagtg
     8701 atggaagaag tggcgaaaat ggcgtggatt gcgcgcagta ttaatccaca actcaatcat
     8761 atcgacagct acctgatgaa taaacacttc atgcgtaagc acggtcctaa cgcttattac
     8821 gggcagaagt aaaacacgcg ctgcggaaat ttccttcctc gggagataac tggtctaatt
     8881 ccgcagccgt ttttcaaaaa aagccccctg cgaagggggc aaagcaaact atggcaatgt
     8941 ttcgttggtt atacctggtg ctagcgataa atatccgcgc tggcgtgcat attgccgtta
     9001 ctccccggtt cccgcatcag aattacgtgg tagtacgttg cgccttgcgc atctgtttct
     9061 tcatttaatg cctgacgtgc ttcgctttca gtggcgaaat tatgattgat ataaatcacg
     9121 cctaagcttt gcacatcgtc catatttctg gcctggtggt tattaatttc aatggctgcc
     9181 catgtatttg cacttagcaa aagcacagcc agaagggcta aaactcgact gaacatagat
     9241 acctcctcga cggctgactt tgtgtgctct cctctgtgat gatcttctga tttaatttta
     9301 atcaatgata aagaagttga tggtgaccat ttctgatgca gttgttcaaa aaaacactgt
     9361 gacggaatgt gatgaacttc aaatcagcgg tttagaggtt atttgcgaaa ggggagattt
     9421 atttctgctc cgcccttgag tttagcgggg tatgccagta ctataacgcg tcatttttca
     9481 gccgaccttt aacacgttcc ttgcctcccc gggattcggc tgacccagac aggaggctga
     9541 ataatccgta aggagcaatt cgatgcgtca ttacgaaatc gtttttatgg tccatcctga
     9601 ccagagcgaa caggttccgg gcatgatcga gcgctacact gctgccatca ctggtgcaga
     9661 aggcaagatc caccgtctgg aagactgggg ccgccgtcag ctggcttacc cgatcaacaa
     9721 actgcacaaa gcacactacg ttctgatgaa cgttgaagct ccgcaggaag tgatcgatga
     9781 gctggaaact accttccgct tcaacgatgc cgttatccgc agcatggtta tgcgtaccaa
     9841 gcacgctgtt accgaagcat ctccgatggt taaagcgaaa gacgagcgcc gtgagcgtcg
     9901 cgatgatttc gcaaacgaaa ccgctgatga tgctgaagct ggggattctg aagagtaatt
     9961 tctgatgacc aaccgtctgg tgttgtccgg caccgtgtgc aggactcccc ttcgaaaggt
    10021 cagtccatca ggaattcctc actgccagtt cgtgcttgag catcgttctg tgcaggagga
    10081 agccggcttt caccggcagg catggtgtca aatgcccgtt attgttagcg gacacgaaaa
    10141 ccaggccatt actcacagta taacggtcgg cagtcgcata accgttcagg ggttcatttc
    10201 atgccacaag gcaaagaacg gactgagcaa aatggttttg catgccgagc agattgaatt
    10261 gatagattct ggagactagc catatggcac gttatttccg tcgtcgcaag ttctgccgtt
    10321 tcaccgcgga aggcgttcaa gagatcgact ataaagatat cgctacgctg aaaaactaca
    10381 tcaccgaaag cggtaagatt gtcccaagcc gtatcaccgg tacccgtgca aaataccagc
    10441 gtcagctggc tcgcgctatc aaacgcgctc gctacctgtc cctgctgccg tacactgatc
    10501 gccatcagta atcggtcacg gtccattaat acgactttga gaggataagg taatgcaagt
    10561 tattctgctt gataaagtag caaacctggg tagcctgggt gatcaggtaa acgttaaagc
    10621 gggctatgct cgtaacttcc tggtaccgca gggtaaagct gttccagcta ccaagaaaaa
    10681 cattgaattc ttcgaagcac gtcgcgctga actggaagct aaactggctg aagttctggc
    10741 agctgctaat gctcgcgctg agaaaatcaa tgcactggaa actgttacca tcgcgtctaa
    10801 agctggcgac gaaggtaaac tgttcggttc catcggtact cgcgacatcg ctgacgctgt
    10861 aactgcagct ggcgttgaag tggctaagag cgaagttcgt ctgccgaacg gcgttctgcg
    10921 taccactggc gaacacgaag tgagcttcca ggttcacagc gaagtattcg cgaaagtgat
    10981 cgtaaacgta gtagctgaat aattcgttat tcaacgagac gtaaaaacgc cgaccattgg
    11041 tcggcgtttt gctttctatt tttcgtcagg cattaatttc gcaagtagat ccattcacca
    11101 ttctcttgtg tccataagtc aggatggttt gctggcgtta gatgcatacg atcactcctt
    11161 atgtctcggt cctgcggcac tcgctcgaaa cctaaacccg agtagattgc ggctgaattt
    11221 gataaggaac gtagaccaat tttcaaatca tggggtaaat cagaatacgc gtttagcaat
    11281 gcagcaatga tgaatccgag ggcattagaa acgcctgtaa cagtgtgaag ttggtcgata
    11341 taaagatcga ttgttggtag ttcagatgag cagcaattag gcatttgggc aggaagggtt
    11401 acaacgtatc ctatcgtttg gccggtatag tctggagtac tataaccagg tcccaaaaca
    11461 acctcgtgta ttgtgtattt actgttgggt tcgaaggatt cgacatcttg caataaatat
    11521 ttcagttctg cggttaacct cgcacccgcg taagctttat tgtatttctg ggcgtgaata
    11581 ttatctaatg tagcttcatg gttccatcca aaagaattat caccattcat tgttactgaa
    11641 tcgccacggg tttaacagac acctcagagt catttaagat gg
//
LOCUS       DAJTSA010000117        11655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000117 DAJTSA010000000
VERSION     DAJTSA010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.117"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            597..794
                     /locus_tag="OO369_003437"
     CDS             597..794
                     /locus_tag="OO369_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983584.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="OO369_003438"
     CDS             complement(744..1937)
                     /locus_tag="OO369_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5983585.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="OO369_003439"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="OO369_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR5983586.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="OO369_003440"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="OO369_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR5983587.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="OO369_003441"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="OO369_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR5983588.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="OO369_003442"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="OO369_003442"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR5983589.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="OO369_003443"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="OO369_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR5983590.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="OO369_003444"
     CDS             complement(10573..10716)
                     /locus_tag="OO369_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983591.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10955..>11655
                     /locus_tag="OO369_003445"
     CDS             10955..>11655
                     /locus_tag="OO369_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5983592.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQW"
BASE COUNT         2956 a         2897 c         2992 g         2810 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctaccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattag
    10801 tattgattca cataatctct catcattaat gagaacattg tcacatattt acaatataga
    10861 actgttactt ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata
    10921 ctttgttcat tcaataaatg agttgagaga atatatgaat aaaatatact cccttaaata
    10981 tagtgctgcc actggcggac tcattgctgt ttctgaatta gcgaaaagag tttctggtaa
    11041 aacaaaccga aaacttgtag caacaatgtt gtctctggct gttgcaggta cagtaaatgc
    11101 agcaaatatt gatatatcaa atgtatgggc gagagactat cttgatcttg cacaaaataa
    11161 aggtattttc cagcccggag caacagacgt aacaatcact ttaaaaaacg gagataaatt
    11221 ctctttccat aatctctcaa ttccggattt ttctggtgca gcagcgagtg gcgcagctac
    11281 cgcaatagga ggttcttata gtgttactgt tgcacataac aaaaagaacc ctcaggccgc
    11341 agaaactcag gtttacgctc agtcttctta caaggttgtt gacagaagaa attccaatga
    11401 ttttgagatt cagaggttaa ataaatttgt tgtggaaaca gtaggtgcca ccccggcaga
    11461 gaccaaccct acaacatatt ctgatgcatt agaacgctac ggtatagtca cttctgacgg
    11521 ttcaaaaaaa atcataggtt ttcgtgctgg ctctggagga acatcattta ttaatggtga
    11581 atccaaaatc tcaacaaatt cagcatatag ccatgacctg ttaagtgcta gtctatttga
    11641 ggtcacccaa tggga
//
LOCUS       DAJTSA010000118        11586 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000118 DAJTSA010000000
VERSION     DAJTSA010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.118"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(282..357)
                     /locus_tag="OO369_003446"
     tRNA            complement(282..357)
                     /locus_tag="OO369_003446"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(322..324),aa:Ile,seq:cat)
     gene            916..3168
                     /locus_tag="OO369_003447"
     CDS             916..3168
                     /locus_tag="OO369_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983593.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3504..4436
                     /gene="csiD"
                     /locus_tag="OO369_003448"
     CDS             3504..4436
                     /gene="csiD"
                     /locus_tag="OO369_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR5983594.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4456..5724
                     /gene="lhgO"
                     /locus_tag="OO369_003449"
     CDS             4456..5724
                     /gene="lhgO"
                     /locus_tag="OO369_003449"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR5983595.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEMIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5747..7195
                     /gene="gabD"
                     /locus_tag="OO369_003450"
                     /pseudo
     CDS             5747..7195
                     /gene="gabD"
                     /locus_tag="OO369_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7203..8489
                     /gene="gabT"
                     /locus_tag="OO369_003451"
                     /pseudo
     CDS             7203..8489
                     /gene="gabT"
                     /locus_tag="OO369_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            8727..10127
                     /gene="gabP"
                     /locus_tag="OO369_003452"
     CDS             8727..10127
                     /gene="gabP"
                     /locus_tag="OO369_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR5983596.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10148..10810
                     /gene="csiR"
                     /locus_tag="OO369_003453"
     CDS             10148..10810
                     /gene="csiR"
                     /locus_tag="OO369_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR5983597.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10811..11260)
                     /gene="kbp"
                     /locus_tag="OO369_003454"
     CDS             complement(10811..11260)
                     /gene="kbp"
                     /locus_tag="OO369_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR5983598.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11344..11502)
                     /locus_tag="OO369_003455"
     CDS             complement(11344..11502)
                     /locus_tag="OO369_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR5983599.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2981 a         2846 c         2889 g         2870 t
ORIGIN      
        1 tccggaacaa tttgaaaacc agaacctcgc ttaggcctgt gtccatatta cgtgggtagg
       61 atcaatttgc ccgcttggca tagcgggcat tttttccagg tacttttgaa tgagtactga
      121 tgaataaata cattgcagtg gcgtgccacg taccaaaaca ccagccctca ttcgaaacca
      181 cccaccgcac ttcttccttg aaatggcgtt agtcatgaaa tatagaccgc catcgagtac
      241 cccttgtacc cttaactctt cctgatacat gaatgatgat ttggtggccc ttgctggact
      301 tgaaccagcg accaagcgat tatgagtcgc ctgctctaac cactgagcta aagggcctta
      361 agtatgcaat aacaatactt ataaaccacg caataaacat gatgatctta tgatgtaaat
      421 aacagatttt tatgcgttcc cattcacctt gggtcgtgat tacacgcgac ataaaacccg
      481 acaccgcctc cattcgcaaa gtcgatactc gcagtcaaca agcaaatgtt aataattagc
      541 actatctata gttatcatcg attcaatgat actttgtaat gattttgtat ctaataatat
      601 aactttatta cattagctga agagttttcg catcattatg atatctgtta cttttcactc
      661 cataaaaata aacttcatat agcaatatat tctttcatag atcttattct gctaatcatt
      721 agtttcgtat gcgcgatttt tgacagttgc ctctccagac cacatcgata attaaaaaac
      781 agatttaaac attatccttt tccatataga tattggataa aaataggaca tctgtttgca
      841 attactttca caacaattaa acatttttat gttttcgtat acatcatatt actctaccat
      901 tagaggaacg ttattatgtt ttctataaaa ccaggacctg gaaatttacc tatcgacaac
      961 cccacattgt tatcatggaa cattactgat ggggatctaa attccaaatt taatacatta
     1021 gaatatctaa actgtataac aaatattatt aatgcttgtg gagtttatcc tcaggattta
     1081 aaagacagag aaattatatc aacttttcac gcagaaaaag tcattaatga tctgttaaaa
     1141 aacgattata aaatttccct ttctccagat acaacttacc gagagctgaa taaagctgca
     1201 cagagtagca ttacagcgcc agacaggata ggagaaggga aaacatgggt ttatcaacga
     1261 gatacaatgg ttgaaagagg tgataacagt ggtgttcatc agtatggtcc agctgaacat
     1321 ttcactcaca ttatatctga caaaccgtcc ccaaaagata agtatgttgc atatgctatt
     1381 aacattcctg actatgagct agcagccgat gtatataata ttaacgtgac gtcaccttcc
     1441 ggacagcaag aaacatttaa aatactaatc aatccagaac atctacggca aacacttgag
     1501 cgtaaatctc ttactgctgt tcagaaatca caatgtgaaa tcatcacccc caaaaaacct
     1561 ggcgaagcga ttcttcatgc ttttaatgcc acctaccagc aaatcaggga aaatatgtct
     1621 gagtttgcac gttcccatta tgggtatata caaatccctc cagtgacaac tttccgcgcc
     1681 gacggaccag aaactcccga agaagaaaaa ggttactggt ttcacgctta tcaacccgaa
     1741 gatctttgta ccatccataa tccaatgggg gatttgcagg attttatcgc attggttaaa
     1801 gatgctaaaa aatttggtat cgatatcatt cctgattata cctttaactt tatgggaatc
     1861 gggggaagtg gtaaaaatga cctggattat ccctctgctg atatacgagc gaagatcagt
     1921 aaagatatag aaagtggtat ccctggctat tggcaaggtc aggttttgat tccattcatt
     1981 aaagatccag taacaaaaga acgtaaacaa atccatccag aagatataca tctcactgca
     2041 aaagacttcg aagcaagtaa agataacatc tctaaggatg aatggaaaaa cctccatgca
     2101 ttaaaagaaa agagtttaaa tggaatgcct aaaacaaccc ccaaaagtga ccaggttatt
     2161 atgttgcaaa atcaatacgt tcgtgaaatg cgaaaatatg gcgtacgagg tttacgttat
     2221 gatgcagcaa aacactcaaa acatgaacaa atagaaagat caataacccc accgcttaaa
     2281 aattataatg agcgattaca caatactaac ttatttaacc caaaatatca tgaaaaaacc
     2341 gttatgaatt acatggaata tctggtaact tgtcagttga atgaagaaca aatgtcatcg
     2401 ctgctttatg aaagagatga tttaagcgcc attgattttt cattgctcat gaagacgata
     2461 aaagcctttt cattcggggg agatctccta acccttgcat caaaaccggg ttccacaatc
     2521 tcaagcatcc catcagaaag acggatattg attaacatta accacgattt tcctaacaat
     2581 ggcaatcttt tcaatgactt tctatttaac catcaacaag atgaacaatt agcaatggca
     2641 tatatggccg ctctcccgtt cagcaggcct ttagtttact gggatggcca ggtattaaaa
     2701 tcaacgactg aaattaaaaa ttatgatggg cccacgcgtg tcggtggtga ggcgtggctt
     2761 aataaaggtt gctctaccta tcagcagctc tacaatgaat tccacgcatt atatatagat
     2821 aaagcaggaa tatggagcgc atttgagggt gtatttgcaa ctaaaaacgt tctggccttt
     2881 agtcgtgggg attctgtgaa cattaatcac tctcctcatg atggactagt tataataaat
     2941 aaaggaaacg aagaagttga aggtacctgg cctaacaaat tgcaacctgg aatatataaa
     3001 aacatgggga gtaatagcgt taacattatt attaataata cccgaaaaat tatcccccct
     3061 tgtaaagtat ttactcttag aggcggatct ctaaatatca atattcctag gcgtagcgct
     3121 cttcttttag ggaaaacagg agaaccgccg aactatctct atttataatt taatgttata
     3181 tctgccccga taaaacgggg cagataatat gtttagttta ctaacggtca ttttgtagtg
     3241 aagccattta ctgtttttta tcgaccagat aatctgttct ctaatgttaa ctccccctaa
     3301 cctgttgctt tagttattca tttcctgtct cactttgcct taatacccta cgttaaatgt
     3361 tactaatttg ttgcttttga tcacaataag aaagcaatat gtcgcttttg tgcgcatttt
     3421 tcagaaatgt agatattttt agattatggc tacgaaatga gcatcgccat gtcaccctac
     3481 atctcataag aggatcgctt ctgatgaatg cactgaccgc cgtacaaaat aacgctgtcg
     3541 attcaggcca ggactatagc ggattcactc tcatcccgtc ggcgcaatcc ccgcgtctgc
     3601 tggaactcac cttcaccgaa cagacgacca aacagtttct cgaacaggtt gccgaatggc
     3661 ccgtgcaggc gctggagtac aaatcgtttc tgcgttttcg ggtaggcaaa attcttgacg
     3721 atctgtgtgc gaatcagctg caaccattgc tgttgaagac cctgctaaac cgtgctgaag
     3781 gtgcgctgtt gatcaatgcg gtaggtatcg atgatgtggc gcaggcagat gagatggtga
     3841 agctggcaac ggcggtagcg catctgattg gtcgctcaaa tttcgatgcg atgagcggtc
     3901 agtattacgc gcgtttcgtg gtgaaaaatg tcgataactc agacagctat ctgcgtcagc
     3961 cgcaccgcgt gatggaactg cacaacgacg gcacgtatgt cgaagagatc accgattacg
     4021 tgctgatgat gaaaatcgat gactgggaac atctggacca ctatttccgc catccactgg
     4081 cgcgtcgccc gatgcgcttt gccgcgccgc cgagcaaaaa cgtcagcaaa gatgttttcc
     4141 atccagtgtt cgacgttgat caacagggtc gcccggtgat gcgctatatc gaccagttcg
     4201 tccagccaaa agattttgaa gaaggcgtgt ggttgagcga gctttccgat gccattgaaa
     4261 ccagcaaagg cattctttct gtacccgttc ccgttggcaa attcctgttg ataaacaacc
     4321 tgttctggct gcacggtcgc gaccgcttta ctccgcaccc ggatctgcgc cgtgaactga
     4381 tgcgtcagcg tggctatttc gcttacgcca ctcaccacta ccagacgcat cagtaagcgc
     4441 aaaggaattg agcggatgta tgattttgtg attattggcg gcggcatcat cggcatgtcg
     4501 accgccatgc aactgattga tgtttatccg gatgcccgca ttgcgttgct ggaaaaagag
     4561 tccggcccgg cctgtcacca gacgggccac aacagcggcg tgatccatgc cggggtctat
     4621 tacacgcccg gcagcctgaa ggcgcagttt tgcctggcgg ggaaccgtgc caccaaagcc
     4681 ttttgcgatc aaaacggcat tcgctatgac aactgcggca agatgctggt cgccacgtct
     4741 cagctcgaaa tggaacggat gcgcgcctta tgggaacgca cagcggcgaa cggtatcgag
     4801 cgcgagtgga taaacgccgt ggaactgcgc gagcgcgaac cgaatatcac cgggcttggt
     4861 ggcatttttg tgccgtccag cggcattgtc agctaccgcg aagtgacggc ggcaatggca
     4921 aaaatcttcc aggccagagg cggcgagatg atttataacg ccgaagtcag cgccctcagt
     4981 gagcataaaa acggcgtggt gatacgcacc cgtcagggcg gtgaatatga agcatcgacg
     5041 ctgattagct gttccgggct gatggctgac cggctggtga aaatgctcgg cctcgaaccg
     5101 ggctttatta tctgcccgtt ccgtggcgag tatttccgcc ttgcgccgga gcataaccag
     5161 attgttaacc acctgattta ccccattccc gaccccgcga tgccattttt gggcgtgcat
     5221 ctcacccgca tgatcgacgg cagcgtgacc gtcgggccaa acgcggtgct ggctttcaaa
     5281 cgcgaaggct atcgcaagcg cgacttctca tttagtgaca cgctggagat tttgagctcg
     5341 tcggggattc gccgggtgct gcaaaaccat ctacgctcag gactgggcga gatgaaaaac
     5401 tcgctgtgca aaagcggcta tctgcggctg gtgcaaaagt attgtccccg gctttcgtta
     5461 agcgatctcc agccctggcc cgccggtgtg cgggcgcagg cagtatcgcc ggacggcaag
     5521 ctgattgacg attttctgtt tgtcaccacc ccacgcacga tccacacctg caatgcgccc
     5581 tccccggcag cgacatcagc aattcctatt ggtgcgcata ttgtcagcaa ggtacaaacg
     5641 ctgttggcaa gccagagtaa ccccggacgc acgctgcgag cggcacgtag tgtggatgcc
     5701 ttacacgccg catttaatca ataacctttg aaaacaggac gtaacaatga aacttaacga
     5761 cagtaactta ttccgccagc aggcgttgat taacggggaa tggctggacg ccaacaatgg
     5821 tgaagtcatc gacgtcacca atccggcgaa cggcgacaag ctgggtagcg tgccgaaaat
     5881 gggcgcagat gaaacccgcg ccgctatcga cgccgccaac cgcgccctgc ccgcctggcg
     5941 tgcgctcacc gccaaagaac gcgccaccat tctgcgcaac tggttcaatt tgatgatgca
     6001 gcatcaggac gatttagcgc gcctgatgac cctcgaacag ggtaaaccgc tggctgaagc
     6061 gaaaggcgaa atcagctacg ccgcctcctt cattgagtgg tttgctgaag aaggcaaacg
     6121 tatttatggc gacaccattc ctggtcatca ggccgataag cgcctgattg ttatcaagca
     6181 gccgattggc gtcaccgccg ccatcacgcc gtggaacttt ccggcggcga tgattacccg
     6241 taaagccggt ccggcgctgg cggcaggctg caccatggtg ctgaagcccg ccagtcagac
     6301 gccgttctct gcgctggcgc tggcggaact ggcgattcgc gcgggcattc cggctggcgt
     6361 gtttaacgtg gtcaccggtt cggcaggcgc ggtcggtaat gaactgacca gcaacccgct
     6421 ggtacgcaaa ctgtcgttta ccggttagac cgagattggc cgccagttaa tggaacagtg
     6481 cgcgaaagac atcaaaaaag tgtcgctgga gctgggcggc aacgcgccgt ttatcgtctt
     6541 tgacgatgcc gatctcgata aagccgtgga aggcgcgctg gcctcgaaat tccgcaacgc
     6601 cgggcaaacc tgcgtctgcg ccaaccgttt atacgtgcag gacggcgtgt atgaccgctt
     6661 tgccgaaaaa ttacagcagg cggggagcaa actgcacatc ggcgacgggc tggagaaagg
     6721 cgtcaccatc gggccgctga tcgatgaaaa agcggtagca aaagtggaag agcatattgc
     6781 cgatgcgctg gagaaaggtg cgcgtgtggt ttgcggcggt aaagcgcacg aacgcggcgg
     6841 caacttcttc cagccaacca ttctggtgga cgttccggcc aacgccaaag tgtcgaaaga
     6901 agagacgttc ggccccctcg ccccactgtt ccgttttaaa gatgaagccg atgtgattgc
     6961 acaagccaat gacaccgagt ttggtcttgc cgcctatttc tacgcccgtg atttaagccg
     7021 cgtcttccgc gtcggcgagg cgctggagta cggcatcgtc ggcatcaata ccggcattat
     7081 ttccaatgaa gtggccccat tcggcggcat caaagcctcg ggtctgggtc gtgaaggttc
     7141 gaagtatggc atcgaagatt acttagaaat caaatatatg tgcatcggtc tttaactgga
     7201 gaatgcgaag gaacagcaat aaagagttaa tgcagcgccg cagtcaggcg attccccgtg
     7261 gcgttgggca aattcacccg attttcgctg accgcgcgga aaactgccgg gtgtgggacg
     7321 tttaaggccg tgagtatctt gatttcgcgg gcgggattgc ggtgctcaat accgggcacc
     7381 tgcatccgaa ggtggtggcc gcggtggaag cgcagttgaa aaaactgtcg cacacctgct
     7441 tccaggtgct ggcttacgag ccgtatctgg agctgtgcga gattatgaat cagaaggtgc
     7501 cgggcgattt cgccaagaaa acgctgctgg ttacgaccgg ttccgaagcg gtggaaaacg
     7561 cggtaaaaat cgcccgcgcc gccaccaaac gtagcggcac catcgctttt agcggcgagt
     7621 atcacgggcg cacgcattac acgctggcgc tgaccggcaa ggtgaatccg tactctgcgg
     7681 gcatggggct gatgccgggt catgtttatc gcgcgcttta tccttgcccg ctgcacggca
     7741 taagcgagga tgacgctatc gccagcatcc accggatctt caaaaatgat gccgcgccgg
     7801 aagatatcgc cgccatcatg attgagccgg ttcagggcga aggcggtttc tacgccgcgt
     7861 cgccagcctt tatgcagcgt ttacgcgctc tgtgtgacga gcacgggatc atgctgattg
     7921 ccgatgaagt gcagagcggc gcggggcata ccggcacgct gtttgcgatg gagcagatgg
     7981 gcgtggcgcc ggatcttacc acctttgcga aatcgatcgc aggcggtttc ccgctggcgg
     8041 gcgtcaccgg gcgcgcggaa gtgatggacg ctgttgcacc gggcggtctg ggcggcacct
     8101 atgccggtaa cccgattgcc tgcgtggctg cgctggaagt gttgaagggg ttcgagcagg
     8161 aaaatctgct gcaaaaagcc aacgatctgg ggcagaagct aaaagacgga ttgttggcga
     8221 tagccgaaaa acacccggag atcggcgacg tacgcgggct gggatcgatg attgccatcg
     8281 agctgtttga agatggcgat cacagcaaac cggacgcaaa actcaccgcc gagatcgtgg
     8341 cacgcgcccg cgataagggc ctgattcttc tctcctgcgg cccgtattac aacgtgctgc
     8401 gcatccttgt accactcacc attgaagacg ctcagatccg tcagggtctg gagattatcg
     8461 gtcagtgttt tgatgaggcg aagcagtagc accgtcccca tgcaggaggc gacgctgcgc
     8521 gtcttgtccg gcctacgaga atccaagtcg gataaggcgt ttacgccgca tccgacaatc
     8581 tgtacgtgaa caggaataaa tctatgtcgg ccggataagg cggagccgct ctccggcaaa
     8641 aagaatcaat aacaattaaa cgcgtgaccc gacgcgggaa atgtcggggc gctctcccaa
     8701 gtgacacact ttcgagagga ttcaggatgg ggcaatcatc gcaaccacac gagttaggcg
     8761 gcgggctgaa atcacgccac gtcaccatgt tgtctattgc cggtgttatc ggcgcaagtc
     8821 tgtttgtcgg ttccagcgtc gccatcgccg aagcggaccc ggcggtatta ctggcctatc
     8881 tgttcgccgg attactggtg gttatgatta tgcggatgct ggcggaaatg gcagttgcca
     8941 cgcccgatac cggttcgttt tccacctatg ccgataaagc cattggccgc tgggcgggct
     9001 ataccatcgg ctggttgtac tggtggtttt gggtactggt tatcccactg gaagccaaca
     9061 tcgccgccat gatcctgcac tcgtgggttc caggcattcc catctggtta ttttccctcg
     9121 tcattaccct cgccttaact ggcagtaatt tattaagcgt taaaaactac ggcgaatttg
     9181 agttctggct ggcgctgtgc aaagtcatcg ctatcctggc ctttattttc cttggtgcag
     9241 tcgcaattag cggtttttac ccttatgccg aagtgagcgg gatctcaaga ttgtgggata
     9301 gcggcggctt tatgcccaac ggtttcggtg cggtattaag cgcgatgttg atcaccatgt
     9361 tctcgtttat gggcgcagaa attgtcacca ttgccgccgc ggaatccgac atgccggaaa
     9421 aacatattgt ccgcgccacc aactcggtta tctggcgtat ttctatcttc tatttgtgct
     9481 ctatttttgt cgtcgtggca ttaataccgt ggaatatgcc ggggctgaaa gccgttggat
     9541 cttatcgctc ggttctggaa ttgctcaata ttccccatgc gaaattaatc atggactgtg
     9601 tgatattact ttccgtaacc agttgcctga actcggccct gtataccgcc tcaaggatgc
     9661 tctactcctt aagccgtcgc ggtgatgctc ccgcggtaat gggtaaaatc aaccgcagta
     9721 aaaccccgta cgtggcggtg ttactctcca ccggcgcagc attcttaacg gtggtggtga
     9781 actattacgc gcctgcgaag gtatttaaat ttctgatcga cagctccggc gctatcgccc
     9841 tgctggttta tttagtcatc gccgtttcac agttgcggat gcgcaaaatt ctgcgagcag
     9901 aaggaagcga aattcgcttg cgaatgtggc ttgatccctg gctcacctgg ctggtcatcg
     9961 gctttattac ctttgtgttg gtagtgatgc tattccgtcc ggcgcaacag ttagaagtga
    10021 tctccaccgg cttattagcg atagggatta tctgtaccgt gccaattatg gctcgctgga
    10081 aaaagctggt attgtggcaa aaaacacccg ttcataatac gcgctgataa tgatcaggag
    10141 tcacaccatg accattacgt ctctggatgg ctatcgctgg ctgaagaacg atattattcg
    10201 cggtaatttt caaccggatg aaaaattacg gatgagttta ctgacatcgc gttatgcact
    10261 tggcgttggg ccgttgcagg aagcactttc tcaactggtg gcggaacggc tggtcacggt
    10321 ggtgaatcaa aaagggtatc gggtggcgtc tatgtcagag caggagctgc tcgatatttt
    10381 cgacgcccgc gccaatatgg aagcgatgct ggtcagtctg gcgattgccc gcggtggcga
    10441 tgaatgggag gcagacgttc tcgcaaaagc gcatctgctc agtaagctgg aagcctgtga
    10501 cgccagcgag aaaatgcttg atgagtggga tctgcgtcat caggcgtttc atacagcaat
    10561 tgtggcgggc tgtggttctc actatttgct gcaaatgcgt gaacggttgt ttgatctggc
    10621 ggcgcgttat cgatttatct ggctgcggcg aacggtgctt tcggtggaaa tgctggagga
    10681 taaacacgat cagcaccaga ccctgactgc ggcggtactg ccgcgagata cagcgcgcgc
    10741 cagtgagtta atgcgccagc atttactgac gccaattccc attatccagc aggcgatggc
    10801 tggcaattaa ctactcttcc ggaatacgca acacttgccc cggataaatt ttatccgggc
    10861 tttttagcat cggtttattc gcttcgaaga ttttattgta cagattagcg ttaccgtaga
    10921 cctgtttgga aatggcactc agagtgtcgc cagacttaac ggtataaaac tggctggcag
    10981 tggctggtgt cgccgttttc acctgatcat caacactggc aataccggaa atattcccca
    11041 ccgcaacaag gattttctcc ttcgcctcct gactcaggcc gtcaccagtg accgtcgctt
    11101 tgccgtcggc aatttgaatg ttcactttat cggcatcagg tataccggtt ttgctcagat
    11161 gctcctgcac cttcttcgcc tgatcgtctt tatcgtgctg acctgtaacc gcatcccaga
    11221 gtttttctcc ggcatcttta acaaaattga acagacccat agctacctca ttcgttaacg
    11281 gaaacactca gaaagtgtag cagaggaacg cggcgcgtta acgccgcggg ggtgagcaca
    11341 ggtttaatcg cgggtttgca cccagaacgc gtgaatcaaa ccggggatat acccaagcag
    11401 tgtcagcaga atattaataa tgaacgccca accgaaccct ttaccgagca gcacgccgat
    11461 cggcggcaga atgatggtga tgacgattct ccagaaaccc atatgtactc cctataagaa
    11521 aattactcat tgtttaaaaa gagattttat ctcttaagcg tagtaatttt agcggagcct
    11581 gccagt
//
LOCUS       DAJTSA010000119        11335 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000119 DAJTSA010000000
VERSION     DAJTSA010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.119"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..611)
                     /gene="hdhA"
                     /locus_tag="OO369_003456"
     CDS             complement(<1..611)
                     /gene="hdhA"
                     /locus_tag="OO369_003456"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCR5983600.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITP"
     gene            complement(723..1751)
                     /gene="malI"
                     /locus_tag="OO369_003457"
     CDS             complement(723..1751)
                     /gene="malI"
                     /locus_tag="OO369_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR5983601.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYVELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1926..3518
                     /gene="malX"
                     /locus_tag="OO369_003458"
     CDS             1926..3518
                     /gene="malX"
                     /locus_tag="OO369_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR5983602.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3528..4700
                     /gene="malY"
                     /locus_tag="OO369_003459"
     CDS             3528..4700
                     /gene="malY"
                     /locus_tag="OO369_003459"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR5983603.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4804..5805
                     /gene="add"
                     /locus_tag="OO369_003460"
     CDS             4804..5805
                     /gene="add"
                     /locus_tag="OO369_003460"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR5983604.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5841..6881)
                     /locus_tag="OO369_003461"
     CDS             complement(5841..6881)
                     /locus_tag="OO369_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5983605.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7124..7249
                     /gene="blr"
                     /locus_tag="OO369_003462"
     CDS             7124..7249
                     /gene="blr"
                     /locus_tag="OO369_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR5983606.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7522..7737
                     /gene="ydgT"
                     /locus_tag="OO369_003463"
     CDS             7522..7737
                     /gene="ydgT"
                     /locus_tag="OO369_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR5983607.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7823..8263
                     /locus_tag="OO369_003464"
     CDS             7823..8263
                     /locus_tag="OO369_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR5983608.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8340..8921
                     /gene="rsxA"
                     /locus_tag="OO369_003465"
     CDS             8340..8921
                     /gene="rsxA"
                     /locus_tag="OO369_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR5983609.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8921..9499
                     /gene="rsxB"
                     /locus_tag="OO369_003466"
     CDS             8921..9499
                     /gene="rsxB"
                     /locus_tag="OO369_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR5983610.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9492..>11335
                     /gene="rsxC"
                     /locus_tag="OO369_003467"
     CDS             9492..>11335
                     /gene="rsxC"
                     /locus_tag="OO369_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR5983611.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEE"
BASE COUNT         2647 a         2924 c         2859 g         2905 t
ORIGIN      
        1 tctggtgtaa taacggattt cagggcatcg gttaatattg cccccggcgc aatgctattc
       61 acccgaatat ttttttcacc caggtcaaat gccatatttc tgaccagatg actggccgca
      121 gctttagatg atgcatagga agtcatgttt atatttttat tttctgccgc catagaagtg
      181 atggtcagaa taacgccacc tccatttttt tccatttctg gcgcaacaag ttgtgacaga
      241 tggaaaaaag aaaacacatt cagttcataa gcacggcgaa aatctgccat tggcatatca
      301 aacggtttag gaccaccgcc accggcgttg ttaaccagga tatcaacttt acccagatta
      361 ctgacggcaa agtctgccag tgcagagagt tcctgttcgg aagtaatatc acaacgccag
      421 gcaaatgcct gaccatccag ttgttgaatt tcgtctacaa catggttagc tgcgtcggcg
      481 ttaatatcac tgaccaccac agatgcgcca gctgtcgcga atgtaatggc gatttcttta
      541 ccaatacctg cacccgcacc tgtgatgatg gcgcattttc cgtcgagtct caggttgtca
      601 gaattaaaca cataaacctc ctgtgaactt cagtaaaaat aaaaggcacc gcctgtgtgc
      661 cacatttttt atcattaacc ttgaggaaag gacactacca agtatagcag ataaagagag
      721 gattatttcg ccgcaatgag ccgggcggga ataataagat tgcgtgaatg cgtctcttca
      781 tgggtgattt tttgcatcat gcgatccgca agtgtgatac caagttcccg cgctggcgtg
      841 ctggcccagg taacaggtat atcatcaagt gtggttggtg tcgcatcggt aaatgccgcc
      901 agcgaaacct gttgctcaaa gtaacgatcg actccgcttt ccccgctttg ccgccctgct
      961 ttcagcaaac caaaccatgc ccccatcgca atagtttcgt tatagcaaac cacggcactg
     1021 atggtcgggt tatgacgtaa aagcgccgtg atagcttccg cggcttgctt ctggctggaa
     1081 gtgcactcca acacccaatc gctgtgaaac ggcaggccaa attttagtag agttgcacaa
     1141 tagcccccca cccgttctgc acgggttaat gaggaacttt gccctcccag ccaggcgatc
     1201 cgctgatgcc cattgcgaat gagatgctcc gtcaacaact gtgcagcctg catgttgtcc
     1261 gggcgaaccg tatcaacatc atcaagataa ctggcacggg aagcgaaaat caccgggata
     1321 gctttttctt ctgccatccg ccgcaggtca tcgctgcttc ctgcagcccc ggcaattacc
     1381 acaccatcga caccctgatt cagtaacagt gaaaaccgct gtgccagctg ctcgccgtct
     1441 ttaccgccgt gaagcaaaaa aaccatccgt ccctgtgctt ccagagcttc cgtcaatccg
     1501 gccgtcaatt cgacgtaaaa cggcgcagat aaatcacgga cgatcaaacc aatgacgccg
     1561 ctttgcccgc cgcgcagcgc cgacgcctgg cgattgcgca caaatcccag ctcttcaatg
     1621 gcggcgttaa cgcgttctcc tgtggcggta gagattcgcc ctttgccatt aagcaccagc
     1681 gaaacggtac ttaccgacac gcccgcagcc agcgcaacat catgaatggt tatttttttg
     1741 gcggtagcca tgcagatgac ctactccctg attatgtgac agataaaacg ttttaccttt
     1801 tatttatctt atacccgcta ttatcgttgc gtaatgtgat ttatgactca ctaaaatttg
     1861 ataaaacgtt ttatcttctc gcgcaattta ctgaatccag attgttctct acgaggagtc
     1921 gttttatgac ggcgaaaaca gcaccgaaag tcacgctgtg ggagttcttc cagcagttag
     1981 gcaaaacctt catgttaccc gtggcattat tgtcgttctg cggcattatg ctcggcattg
     2041 gtagttctct tagcagccat gatgtcataa ccctgatccc ggtcctgggc aaccccgtgt
     2101 tgcaggctat ctttacctgg atgagtaaga ttggctcgtt tgcttttagt ttcctgcctg
     2161 tcatgttctg tatcgccatc ccgctgggcc tggcacgcga aaataaaggc gtagcggcat
     2221 tcgctggctt cgtcggttat gcggtaatga acctcgcggt aaacttctgg ttgaccaata
     2281 aaggcattct gccaaccaca gatgccgccg ttctgaaagc caataacatc cagagcattc
     2341 ttgggatcca gtcgatcgat accgggatcc tcggtgcggt gatcgccggt attatcgtct
     2401 ggatgctgca tgagcgtttc cataatatcc gcctgccgga tgcgctggca ttcttcggcg
     2461 gtacgcgctt cgtaccaatt atctcctcgc tggtgatggg ccttgtcggc ctggtgattc
     2521 cattagtctg gccgattttc gccatgggta ttagcggctt gggccatatg ataaacagcg
     2581 cgggtgattt cggaccgatg ctgtttggta ccggtgaacg tctgctgttg ccgtttggtc
     2641 tgcatcacat tctggtggca ttaattcgct ttaccgacgc aggcggcacg caggaggtct
     2701 gcggtcaaac cgtcagcggc gcactgacca tcttccaggc gcaattgagt tgcccgacca
     2761 ctcacggttt ttctgaaagc gtcacgcgtt tcctttcgca aggtaaaatg cctgcgtttc
     2821 tcggcggtct accaggtgca gcgttagcta tgtatcactg cgcgcgcccg gaaaatcgcc
     2881 ataaaattaa aggtctgctg atttctggcc tgatcgcctg cgtcgttggc ggcactaccg
     2941 aaccgctgga attcctgttc ctgttcgtag cgccagttct gtatgtcatc cacgcgctgt
     3001 taaccggcct cggcttcacc gtcatgtctg tgctcggcgt caccatcggt aataccgacg
     3061 gcaatatcat cgacttcgtg gtgttcggta ttttgcatgg tctgtcaacc aagtggtaca
     3121 tggtgccagt ggtggcggca atctggtttg tcgtttacta cgtcatcttc cgtttcgcta
     3181 tcacccgctt caatctgaaa accccggggc gcgatagcga agttgccagc tcaatcgaaa
     3241 aagccgttgc cggtgcgccg ggtaaatcag gttacaacgt tcctgcaatc ctcgaagcat
     3301 taggcggtgc cgacaatatt gtcagcctcg ataactgcat tacccgtctg cgtttgtctg
     3361 tggaagatat gtcgcttgtt aatgtgcagg cactgaagga caatcgggca attggcgtag
     3421 tacaacttaa tcaacataac ctgcaggttg ttatcgggcc acaagttcag tcagtaaaag
     3481 atgaaatggc cggtctgatg catactgtcc aggcataagg ataagatatg ttcgattttt
     3541 caaaggtcgt ggatcgtcat ggcacatggt gtacacagtg ggattatgtc gctgaccgtt
     3601 tcggcactgc tgacctgtta ccgttcacga tttcagacat ggattttgcc actgccccct
     3661 gcattatcga ggcgctgaat cagcgcctga tgcacggcgt atttggctac agccgctgga
     3721 aaaacgatga gtttctcgcg gctattgccc actggttttc cacccagcat tacaccgcaa
     3781 ttgatacgca gacggtggtg tatggccctt ctgtcatcta tatggtttca gaactgattc
     3841 gtcagtggtc tgaaacaggt gaaggcgtgg tgatccacac gcccgcctat gacgcatttt
     3901 acaaggccat tgaaggtaac cagcgcacag taatgcccgt tgctttagag aagcaggctg
     3961 acggctggtt ttgcgatatg ggcaagctgg aagccgtttt ggcgaaacca gaatgtaaaa
     4021 ttatgctcct gtgtaaccca cagaatccta ccgggaaagt gtggacgtgc gatgagctgg
     4081 agatcatggc tgacctgtgc gagcgtcatg atgtgcgggt tatttccgat gaaatccata
     4141 tggatatggt ctggggcgag cagccgcata ttccctggag taatgtggcg cggggagact
     4201 gggcgttgct gacatcggga tcgaaaagtt tcaatattcc cgcgctgacc ggtgcttacg
     4261 ggattataga aaatagcagc agccgtgatg cctatttatc ggcactgaaa ggacgtgatg
     4321 ggctttcttc cccttcggta ctggcgttaa ctgcccatat cgccgcctat cagcaaggtg
     4381 caccgtggct ggatgcctta cgcgtctatc tgaaagataa cctgacgtat atctcagata
     4441 aaatgaacgc cgcgtttcct gaactcaact ggcagatccc gcaatccact tatctggcct
     4501 ggcttgatct acgtccgttg aatattgacg acaacgcgtt gcaaaaagcg cttatcgagc
     4561 aagaaaaagt cgcgatcatg ccggggtata cctatggtga agaaggtcgt ggttttgtcc
     4621 gtctcaatgc cggctgccca cgttcgaaac tggaaaaagg tgtggctgga ttaataaacg
     4681 ccatccgcgc tgttcgctaa ccccaattgc gcaacgtaaa aaatcgttgc gcaatcgtgg
     4741 atttttaccc tgctttgttt ttataatggt gcgcactttt atatccagaa aaagagtgcg
     4801 accatgattg ataccaccct gccattaact gatatccatc gccaccttga tggcaacatt
     4861 cgtccccaga ccattcttga acttggccgc cagtataata tctcgcttcc tgcacaatcc
     4921 cttgaaacac tgattcccca cgttcaggtc attgccaacg aacccgatct ggtgagcttt
     4981 ctgaccaaac ttgactgggg cgttaaagtt ctcgcctctc ttgatgcctg ccgccgcgtg
     5041 gcatttgaaa acattgaaga tgcagcccgt aacggcctgc gctatgtcga gctgcgtttt
     5101 tcaccaggct acatggcaat ggcacataag ctgcctgtag cgggtgttgt cgaagcggtg
     5161 atcgatggcg tacgtgaagg ttgccgcacc tttggtgtgc aggcgaagct tatcggcatt
     5221 atgagccgaa ccttcggcga agccgcctgt cagcaagagc tggaggcctt tttagcccac
     5281 cgtgaccaga ttaccgcact tgatttagct ggtgatgaac ttggattccc gggaagtctg
     5341 ttcctttctc acttcaaccg cgcgcgtgat gcgggctggc atattaccgt ccatgcaggc
     5401 gaagctgccg ggccggaaag catctggcag gcgattcgtg aactgggtgc ggagcgtatt
     5461 ggacatggcg taaaagccat tgaagatcgg gcgctgatgg attttctcgc cgagcaacaa
     5521 attggtattg aatcctgtct gacctccaat attcagacca gcaccgtagc agagctggct
     5581 gcacatccgc tgaaaacgtt ccttgagcat ggcattcgtg ccagcattaa cactgacgat
     5641 cccggcgtac agggagtgga tatcattcac gaatataccg ttgccgcgcc agctgctggg
     5701 ttatcccgtg agcaaatccg ccaggcacag attaatggtc tggaaatggc tttcctcagc
     5761 gcagaggaaa aacgcgcact gcgagaaaaa gtcgccgcga agtaacaaaa atggatggtg
     5821 caaattgcac catccatttt tcacgcaagg cacaacgtcg cgcgatgttt ggcggattcg
     5881 atgcccagct caatcaactc cattacctgg attgcctggc ttgccggaac cggattttca
     5941 ccatcgccat ttaacgcatc acgaatcgcc gcatagtaag ccggataatt cccaggcacc
     6001 gtcaacagcg tttcttcgac acgttcctca ccttccacgc gggtaagtac gccatcacgc
     6061 atatcgtagc cccagtcttc ctgcggcaga cgctcgccat ttttcagacg ttcttcctgt
     6121 ggatcgaggc catatttcac ataactgcct cgggatccat gcacgatata ccgtgctgac
     6181 tcagcggctg ccagcatggt accgtgtaaa atgactcgcc gctgtggata ggacaagatg
     6241 gcgtggaaat aatcggtcga ctgcgctccg ggccgtaact gtgccaaatc taccgtcatg
     6301 ctgaccggta aaccaaatag cgtaatggcc tgatcaagaa gatgcggtgc taaatcgtac
     6361 cagataccgc tgcctgggcc gccctgttca cgccaacgat cgcgcacctg cggacggaag
     6421 cggtcaaaat gagactcaaa gtaagcaact tcacccagca cgccttccgc gagcaaacct
     6481 tttagcgtca agaaatcgct atcccaacgg cggttatgga atacagacag cacacgcccc
     6541 aggctttttg ccagcgcatc cagctctcgc gcttgtgaca gtgtcacggt aaagggttta
     6601 tcaacgacca catgtttacc cgcctcaagc gccgctttgg ctaacgggaa atgggtatcg
     6661 ttgggtgtag gaatgacaat caggtctatg ttgggatcgt taaacagatg cttcggctca
     6721 gagacaaccg taaccgttgg ccagtcggct tttacttttg tttcatcact gctggagatt
     6781 accgccagtt ccagcccggg cgtgcccgca atcaggggcg catggaaggt tttgctcgca
     6841 taaccatacc caatcaaccc aacacggatg ttgtcgctca tgtcattgcc tctcattgaa
     6901 gtatgatggc tatttgacac tatcctttac ccacgctcaa cagtttaata acctgccagc
     6961 aataagggat gttgtttaac ttaagtcaaa aaaatagcga attttccaac gacaaaagct
     7021 aaatatcgca aaaacctcag taaaaatctt gctggagcta ttattgctaa gtaacattta
     7081 ccccctgaag ttaatggatc aatcaagaga gatgtgggct gtaatgaatc gtcttattga
     7141 attaacaggt tggatcgttc ttgtcgtttc agtcattctt cttggcgtgg cgagtcacat
     7201 tgacaactat cagccacctg aacagagtgc ttcggtacaa cacaagtaag ctctgcactt
     7261 gtggagcgac atgctgcccg tccgggtgca tgttttcact tgtcggatat taaaccagga
     7321 gtttattatc ttgttcgatg ttgttggtga ttgtcaggga tagtaaagcg cgttactctt
     7381 ttcagagcag tttttcatcc ctgaatattt gccatcagcg cattcttatt attgtaataa
     7441 cacaagcgtt cacttttggt gaaattttat catcactaat aatacctacc cctttggtat
     7501 taattatttt atggaccttt tatgactgtt caggactact tattaaaatt tcgcaaaatc
     7561 agttcactcg aaagtctgga aaaactctac gaccatctta attacaccct gacggacgat
     7621 caggaactga tcaatatgta tcgtgctgcc gatcaccgtc gcgcagagct ggtttctggc
     7681 gggcgtttgt ttgacctcgg ccaggtaccg aagtccgtct ggcactatgt ccaataaaga
     7741 aagtagcgat cggttataaa agccttataa taacgcccct aaaattagat ttgcctgttt
     7801 gcgtcgctat tctggagaac ctatgaccac cacaacgcca caacgtattg gaggctggtt
     7861 gcttggcccg ttggcctggc ttttagtcgc tctgttgagt acgacgctgg cgttgttgct
     7921 gtataccgct gcgttatctt ctccgcaaac atttcaaacg ctcggcggac aagcactgac
     7981 gacacaaatc ttatggggcg tttcttttat taccgctatc gccatgtggt attacacact
     8041 gtggttgacc atcgctttct ttaaacgtcg gcgttgtgtt cctaaacact atattatctg
     8101 gctgcttatt tccgtattgc tggcagtaaa agcattcgct ttttcacccg tggaagttgg
     8161 catagccgtt cgccagttgc tgtttacttt gttggcaacc gcattgatcg tcccctattt
     8221 caaacgctcg tcgcgggtaa aagcgacgtt tgtgaatccg taataacctt acagttaacc
     8281 tgttgtcgcc tgctctggat taacggataa taggcggctt ttttatttca ggccgaaaaa
     8341 tgactgacta cctgttactg tttgtcggaa ctgtactggt caataacttt gtactggtca
     8401 agtttctcgg tctctgtccg tttatggggg tttccaaaaa actggaaacc gcgatgggca
     8461 tggggctggc aacaacgttt gtgatgacgc tgacgtctat ttgcgcctgg cttatcgata
     8521 cgtggatttt gatcccactt aatctgattt acctgcgcac cctggcattt attctggtga
     8581 ttgctgtggt cgtgcagttc accgagatgg tggtgcgcaa aaccagcccg gtgctttacc
     8641 gtctgctggg gatttttttg ccgcttatca ccaccaactg tgcagtgctc ggcgtggcgt
     8701 tgctgaatat caatctcggg cacaatttct tgcagtcggc gctgtacggt ttttccgccg
     8761 ctgtcggttt ctcgctggtg atggtgctct tcgccgccat ccgcgaacgc cttgctgtgg
     8821 ctgatgtccc ggcacctttt cgcggtaatg ccattgcgtt aattaccgca ggtcttatgt
     8881 ctctggcctt tatgggcttt agtggtttgg tgaagttgta atgaatgcta tctggattgc
     8941 cgttgccgcc gtgagcctgc tgggcctggc gtttggcgcc attctgggtt atgcctcccg
     9001 ccgttttgcg gtggaagacg atccggtcgt tgagaaaatt gacgaaatct taccgcagag
     9061 ccagtgtggt cagtgcggtt atcccggctg tcgcccctac gcggaagcca tcagctgtaa
     9121 cggtgaaaaa atcaaccgtt gcgccccagg tggcgaagct gtgatgctaa aaattgccga
     9181 gttgcttaat gtcgagccgc agccgctgga tggcgaagcg caagagctaa cgcctgcgcg
     9241 gatggtggcg gttattgatg aaaataactg tattggctgc actaaatgta ttcaggcttg
     9301 tccggtagac gccatcgttg gcgctacccg tgtcatgcat acggtaatga gtgatctctg
     9361 tacgggctgc aatttatgtg ttgatccgtg cccgacgcac tgcatctcgt tgcaaccggt
     9421 cgcagaaaca cctgactcct ggaaatggga tctgaacacc attcccgtgc gtatcattcc
     9481 cgtggaacac catgcttaag ttattctctg cattcagaaa aaataaaatc tgggatttca
     9541 acggcggcat ccatccaccg gagatgaaaa cccagaccaa cggtaccccc ctgcgccagg
     9601 tactactggc gcagcatttt gttattccac tgaaacagca tattggcgct gaaggtgagt
     9661 tgtgcgttag cgtcggcgat aaagtattgc gcggccagcc gcttacccgt ggtcgcggca
     9721 aaatgctgcc tgttcacgcg cccacctcgg gtaccgttac ggctattgcg ccccactcta
     9781 cggctcatcc ttcagcttta gctgaattaa gcgtgattat tgatgccgat ggtgaagact
     9841 gctggatccc gcgcgacggc tgggtcgatt atcgctctcg cagtcgcgaa gagttaatcg
     9901 agcgcataca tcagtttggt gttgccgggc tgggcggtgc cggattcccg acaggcgtta
     9961 aattgcaggg tggtggagat aagattgaaa cgttgattat caacgctgct gagtgcgagc
    10021 cgtacattac cgccgatgac cgtttgatgc aggattgcgc ggctcaggtc gtagagggta
    10081 ttcgcattct tgcgcatatt ctgcagccac gcgaaattct tatcggcatt gaagataaca
    10141 aaccgcaggc gatttccatg ctgcgcgcgg tgctggcgga ctctaacgat atttctctgc
    10201 gggtgattcc aaccaaatat ccttctggcg gtgctaaaca attaacctac attctgaccg
    10261 ggaagcaggt tccacatggc gggcgttcat ccgatatcgg cgtattaatg caaaacgtcg
    10321 gcactgctta tgcagtgaaa cgtgccgtta ttgatggcga gccgattacc gagcgtgttg
    10381 taaccctgac tggcgaagca atcgctcgcc cgggcaacgt ctgggcacgg ctggggacgc
    10441 cagtgcgtca tttattgaat gatgccggat tctgcccctc tgccgatcaa atggtgatta
    10501 tgggtggccc gctaatgggc tttaccttgc catggctgga tgtcccggtc gtaaagatta
    10561 ccaactgtct gttggctccc tctgccaatg aacttggcga accacaggaa gaacaaagct
    10621 gcatccggtg tagcgcctgt gctgacgcct gccctgtgga tcttttgccg caacagttgt
    10681 actggttcag caaaggtcag caacacgata aagctaccac gcataacatt gccgactgca
    10741 ttgaatgtgg ggcttgcgcg tgggtttgcc cgagcaatat tcccttggtg caatatttcc
    10801 gtcaggaaaa agctgaaatt gcggctattc gtcaggaaga aaagcgcgcc gcagaagcca
    10861 aagcgcgttt cgaagcgcgc caggctcgtc tggagcgcga aaaagcgact cgccttgaac
    10921 gacataagag cgcagccgtt caacctgcag ccaaagataa agatgcgatt gctgccgctc
    10981 tggcgcgggt gaaagagaaa caggcccagg ctacacagcc tattgtgatt aaagcgggcg
    11041 aacgcccgga taacagtgca attattgcag aacgggaagc ccgtaaagcg caagccagag
    11101 cgaaacaggc agaactggag caaactaacg acgcagcaac cgttgctgaa ccacgtaaaa
    11161 ctgccgttga agcagctatc gcccgctcca aagcgcgcaa gctggaacag caacaggcta
    11221 atgcggaacc agaagaacag gtcgatccgc gcaaagccgc cgtcgaagcc gctattgccc
    11281 gcgccaaagc acgcaagctg gaacagcaac aggccaatgc ggagccagaa gaaca
//
LOCUS       DAJTSA010000120        11271 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000120 DAJTSA010000000
VERSION     DAJTSA010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11271
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.120"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            327..1967
                     /gene="trg"
                     /locus_tag="OO369_003468"
     CDS             327..1967
                     /gene="trg"
                     /locus_tag="OO369_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR5983612.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAVSMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2005..2928)
                     /locus_tag="OO369_003469"
     CDS             complement(2005..2928)
                     /locus_tag="OO369_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5983613.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3145..4488
                     /locus_tag="OO369_003470"
     CDS             3145..4488
                     /locus_tag="OO369_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5983614.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4713..6368
                     /gene="opgD"
                     /locus_tag="OO369_003471"
                     /gene_synonym="mdoD"
     CDS             4713..6368
                     /gene="opgD"
                     /locus_tag="OO369_003471"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR5983615.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6508..6732
                     /locus_tag="OO369_003472"
     CDS             6508..6732
                     /locus_tag="OO369_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR5983616.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6795..7331
                     /gene="rimL"
                     /locus_tag="OO369_003473"
     CDS             6795..7331
                     /gene="rimL"
                     /locus_tag="OO369_003473"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR5983617.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7326..8306)
                     /locus_tag="OO369_003474"
     CDS             complement(7326..8306)
                     /locus_tag="OO369_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983618.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8430..9422
                     /gene="tehA"
                     /locus_tag="OO369_003475"
     CDS             8430..9422
                     /gene="tehA"
                     /locus_tag="OO369_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR5983619.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9419..10012
                     /gene="tehB"
                     /locus_tag="OO369_003476"
     CDS             9419..10012
                     /gene="tehB"
                     /locus_tag="OO369_003476"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR5983620.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10315..10971
                     /locus_tag="OO369_003477"
     CDS             10315..10971
                     /locus_tag="OO369_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR5983621.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(10989..11267)
                     /gene="tnpA"
                     /locus_tag="OO369_003478"
                     /pseudo
     CDS             complement(10989..11267)
                     /gene="tnpA"
                     /locus_tag="OO369_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         2906 a         2637 c         2838 g         2890 t
ORIGIN      
        1 tgatagaaat atcggtcggg gccttcgtct ttctgattcc cggttagcct gaaaacagaa
       61 agtctcaggc acccgcaggc atcctatgag gtttccttag ggacgaaaat aatcacttca
      121 cgaaattgcg tgctgttttc cagaattttt cgtcattcgg gttagccagt ttagccattc
      181 gttactctct tcattccaat agcattaatt ttctctgcaa taattgttgt aaaaatgtga
      241 cgcaaagagg tttttggtca taagtaatta ccgtcaagtg ccgatgactt tctatcagga
      301 gtaaacctgg acgagagaca acggtaatga atacaactcc ctcacagcga ttaggttttt
      361 tgcatcacat caggctggtt ccgttatttg cctgcattct tggcggtatc ttagttctat
      421 tcgcattaag ttctgctctg gctggctatt tcctctggca ggccgatcgc gatcagcgtg
      481 atgttactgc ggagattgag attcggaccg ggttagcgaa cagttcagat tttttgcgtt
      541 cagcccggat caatatgatt caggccgggg ctgcgagtcg tattgcggaa atggaagcaa
      601 tgaagcgaaa tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg
      661 cttatcagaa tcgaccggtg aaaacacctg ctgatgaagc cttcgacact gaattaaatc
      721 aacgctttca ggcttatatc acgggtatgc aacctatgtt gaaatatgcc aaaaatggca
      781 tgtttgaagc gattatcaat catgaaagtg agcagatccg accgctggat aatgcttata
      841 ccgatatttt gaacaaagcc gttaagatac gtagcaccag agccaaccaa ctggcggaac
      901 tggcccatca gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc
      961 tggtcatgac gctgataaca tttatggtgc tacgtcggat cgtcattcgt ccactgcaac
     1021 atgccgcaca acggattgaa aaaatcgcca gtggcgatct gacgatgaat gatgaaccgg
     1081 cgggtcgtaa tgaaatcggt cgcttaagtc gtcatttaca gcaaatgcag cattcactgg
     1141 ggatgacagt agggactgtt cgacagggcg cggaagagat ttatcgtggc accagcgaaa
     1201 tttcagctgg caatgcggac ctgtcatctc gcaccgaaga acaagcggcg gctatcgaac
     1261 aaactgccgt cagcatggag caactcactg cgacggtgaa acagaatgcg gataacgcgc
     1321 atcatgccag caaactggcg caagaggctt ctattaaagc cagcgatggc gggcagacgg
     1381 tttccggtgt agtaaaaacg atgggcgcta tctccacgag ttcgaagaaa atttctgaga
     1441 tcaccgccgt catcaacagt attgctttcc agacgaatat tctggcactg aatgctgccg
     1501 ttgaagccgc gcgagcgggt gagcaagggc gtggatttgc cgttgtcgcc agcgaagtac
     1561 ggacactcgc aagccgcagc gctcaggcag cgaaagagat tgaaggcttg atcagtgaat
     1621 cagtcaggtt aattgacctg gggtcggatg aggtggcaac ggcagggaaa accatgagca
     1681 ctattgttga tgccgtcgcg agtgttacac atatcatgca ggaaatcgcc gccgcctcgg
     1741 atgaacaaag tagaggcata actcaggtta gccaggcgat ttctgaaatg gataaggtga
     1801 cgcaacagaa tgcttctctg gtagaagagg cctcagcagc ggcggtgtcc cttgaagaac
     1861 aggctgcacg attaactgag gcggtgaacg tattccgtct gaacaaacat tctgtgtcgg
     1921 cagaacctcg cggagcgggt gaaccagtta gtttcgctac ggtgtgaaaa tgttcaagga
     1981 gggatcgaca gatcccttca cctttcagaa cggcattgat tttcgaatag cgttaatcat
     2041 caactggcaa ccagaagaga acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc
     2101 tgcaccatgt cccggaacag gcagggccac cagcgtggca tgacgcaggt cgtcttttac
     2161 agcgccagaa gggacaaacc acacgtaatc gtattcaacg gtaagttgac gagatagcga
     2221 agtagacagc gtttcgatac aacccgaagg aattttacag ccctggctct gcactaatgc
     2281 atctgaatgc tggcgtggcg cagtgccttc tggtgataca acgaccggcc attccagcac
     2341 ccggcttagc gttacgttct cctgaagtag cgggtgatta gggcggacaa ccagcttcaa
     2401 cgattcaaga aacagcagtt cgtaattaag cccggtcatc agttcaggat ctgacatccg
     2461 accaatgccg atatcgattt ccccggtttt taaacccgcc agaatcatag ggttactcat
     2521 tgtcgcaact tgcaaggtcg tctctttttg ttgctgatga aactgaccta taaccgaagg
     2581 taatatcccc agtgccgcag taggtagtgc accaaccctg acgacatcat tattaagacc
     2641 ttctttacga tgaagcgact gtccggcagt gttgatggcg tcaagaactc tgactgcatg
     2701 cgttaaaaat tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa
     2761 gcgagcgccc gtcagctgct ccagttcatt caatgtatta gagagcgcag gttgactcaa
     2821 attaagggtt tcagctgcgc gccccaaagt tccttgttgt gcgacagcta cgaatgtatg
     2881 aaggtggcgc aaacggatgc gctgactaaa cagactattt ttttccataa gcgatgttaa
     2941 aaacgaagcg gtgtcgctga caagtgaagt tgtttgatta tgataacttg attgcaaaat
     3001 attattaaca attaaagcaa ttatgttaca gcaaaatgga taatattgat gttttcgcgg
     3061 cgagatcaca gtttgtaaat tcttcccgcc agagtgaatg cggttaccta cactccagat
     3121 tactgaccac tggaggcaga cactatggcg aacagcatca cggcggatga gattcgggaa
     3181 cagttttcgc aggcaatgtc agccatgtac cagcaagaag ttccgcaata tggcacgctg
     3241 ctggaactgg tagctgatgt gaatctggct gtgctggaaa acaatcctca actgcacgaa
     3301 aaaatggtaa atgcagacga gctggcgcga ctgaatgttg aacgtcatgg ggcgattcgc
     3361 gttgggactg cacaagagct tgctactctt cggcggatgt ttgccattat ggggatgtac
     3421 ccggtgagct attacgatct ctcgcaggca ggggtgccgg tacattcgac agcatttcgg
     3481 cccattgatg atgcttctct ggcgcgtaat cccttccgcg tttttacatc cttactccgc
     3541 cttgagctta tcgagaacga aattttgcgc cagaaagcgg cggagattct gcgtcagcgc
     3601 gatatcttca ccccacgttg tcgacaactg ttagaggaat atgagcagca gggcggtttt
     3661 aacgaaacac aggcacagga gtttgtgcag gaagccctgg aaacgtttcg ctggcaccag
     3721 ttagcaacgg tagatgaaga aacctatcgc gccttgcaca acgaacatcg gttgattgct
     3781 gatgtggtct gttttcctgg atgccatatc aaccacctga cgccacgtac gctggatatt
     3841 gaccgggtgc agtcgatgat gcctgaatgc ggaattgaac ccaaaattct catcgagggg
     3901 ccgccgcgcc gcgaggtacc gattttacta cgccagacca gctttaaagc actggaagag
     3961 acggtgttgt ttgcggggca gaaacagggc acgcataccg cgcgctttgg tgaaattgag
     4021 cagcgtggcg tggcattaac gccgaaaggg cgacaactgt atgatgatct tctgcgtaac
     4081 gctggaaccg ggcaggataa tctcactcac caaatgcatt tacaggaaac cttccgcact
     4141 tttcctgaca gtgagttttt aatgcgtcag caagggttgg catggttccg gtaccgtctg
     4201 acgccttcgg gtgaggcgca tcgtcaggcg attcatcctg gagacgatcc acagccctta
     4261 attgaacgtg gttgggtagt ggcgcaaccc atcacctatg aagatttctt gcccgttagc
     4321 gcggcgggga tcttccagtc aaatctgggt aatgaaacgc agacacgcag tcacggtaat
     4381 gccagtcgcg aagcatttga gcaggcgttg ggttgtccgg ttttggatga gttccagctt
     4441 taccaggaag cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat tctgaccatc
     4501 cgcctttgca aaaatttgcc tgattttaca aacgaatcag gctcatccca tcgacataaa
     4561 aaaaatgccg atttatgcat attctctcag ttcaacaatt ggattattaa taaatattgt
     4621 ctagagtgag cggtcataaa taagcacttt cttgccgctg aaaacgacca gcgcgggacc
     4681 attcacaaca ccagaaggac tcactttcag gtatggatcg tagacgattt attaaaggtt
     4741 caatggctat ggccgccgtg tgcggtacca gcggcattgc ttctcttttt tctcaggcag
     4801 cattcgcggc agattctgat attgccgacg ggcaaaccca gcgttttgac ttctccattc
     4861 tacagtcaat ggcgcacgac ttagcgcaaa cagcgtggcg tggtgcgcct cgtccgttac
     4921 ctgacacgct ggcgacaatg acgccgcagg cttataacag tattcaatac gacgccgaaa
     4981 aatcgctctg gcataacgtt gagaaccgtc aactggacgc tcagttcttc catatgggaa
     5041 tgggattccg tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat ctggcgcgtg
     5101 aaattcactt tcgcccggag ttgttcaaat acaacgatgc aggtgttgat accaaacaat
     5161 tagaagggca aagcgatctc ggctttgccg gttttcgcgt gtttaaagcc cccgaactgg
     5221 cgcgccgtga tgtagtatca tttctcggcg cgagttattt ccgcgccgtt gatgatacat
     5281 atcaatacgg tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac agtaaagaag
     5341 agttccccga ctttaccgcc ttctggtttg atacggtaaa accgggggca actaccttta
     5401 ccgtttatgc gttgctcgat agcgccagca ttactggtgc ctataagttc actatccatt
     5461 gtgagaaaag tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc aaagacatta
     5521 aacagctggg catttcgccg atgaccagta tgttcagctg cggtactaat gaacgtcgga
     5581 tgtgcgatac aattcatccg caaattcatg actctgatcg tctgtccatg tggcggggca
     5641 acggcgagtg gatttgccgt ccgctgaata atccgcaaaa attgcagttc aatgcttaca
     5701 ccgacaacaa cccgaaaggg tttggtttat tgcaactgga tcgtgacttc tcccattatc
     5761 aggacattat gggctggtat aacaaacgcc caagtctgtg ggtggaaccg cgtaacaagt
     5821 ggggtaaggg caccatcggc ctgatggaaa tcccaacaac gggcgaaacg ctggataaca
     5881 ttgtctgctt ctggcagcca gaaaaagctg taaaagcagg tgatgagttt gcattccagt
     5941 atcgtctgta ctggagtgcg caaccgcctg ttcattgccc attagcgcgc gttatggcga
     6001 cgcgtaccgg catgggcggt ttcccggaag gttgggcgcc aggtgaacac tatcccgaaa
     6061 aatgggcgcg tcgttttgcc gtcgatttcg ttggtggtga tctgaaagct gccgcgccaa
     6121 aaggcattga gccggtgatt acgctttcca gtggggaagc gaagcaaatc gaaattctct
     6181 atattgaacc catcgatggt tatcgtattc agtttgactg gtatccgact tcggactcca
     6241 ctgatccggt cgatatgcgg atgtatctac gttgtcaggg ggacgctatc agtgaaacat
     6301 ggctgtatca gtatttcccg ccagcgccgg ataaacgtca gtatgttgac gaccgcgtga
     6361 tgagttaatc gttttttctt cggcaccttc ttcgggaggt gccgtctggt taaacacgat
     6421 cccgctcgca tttttcccta agttaaatga gtaatctgat ggtgtgtatt tcagatacac
     6481 cgtgtcagcc actaacaggg agagcgtatg tttccagaat accgagattt aatatcccgt
     6541 ctgaaaaacg aaaatcctcg ctttatgtcc ttgttcgata aacacaataa acttgatcat
     6601 gaaattgcca gaaaggaagg ttccgacggt cgagggtaca atgcggaagt ggtccgcatg
     6661 aaaaaacaaa agctacagtt aaaagatgag atgctcaaaa tcctgcagca ggagagcgtc
     6721 aaagaggtgt aaactttcct aagccgccag ccaggcggct tttttaacaa cagcatggat
     6781 tgactggaga taagatgact gaaacgataa aagtaagcga atcacttgaa ttacatgctg
     6841 ttgcggaaag tcacgtcaca cctctttatc agttaatctg taaaaataaa acctggttac
     6901 agcagtcgct aaactggccg cagttcgttc agagtgaaga ggacacgcga aaaacggtgc
     6961 agggtaatgt gatgttgcat caacgcggct atgccaaaat gttcatgatt ttcaaagaag
     7021 atgaacttat cggcgttatc tcgtttaatc gtattgaacc actgaataaa actgctgaaa
     7081 tcggctactg gctggacgaa tctcatcagg ggcaggggat catttctcag gcgctgcaga
     7141 cattgattca tcattacgct cagtctggtg aacttagacg cttcgtgatc aaatgtcggg
     7201 tggacaatcc gcaaagcaac caggtcgctt tgcgcaatgg ttttatcctt gaaggttgcc
     7261 tgaaacaggc tgagttcctg aatgatgcct atgatgatgt gaacttatac gcgcgtatta
     7321 tcgattcata ataaccctgc cagcggcgtt cgcgtaatgc gctcctcgcc gttaatgact
     7381 tttggaccac ggacatgaac cgtgtcgcca tcaaaggctt caacgacggc gtggtcggtt
     7441 aactccacat ggttttcaat tatcaccgca cctgtaatac ggctctcacc ttgtattaca
     7501 acgtgctcgt cgagcagaat cggtccacca cgtaccacgg catttccgcc aatcaagaca
     7561 tgatgtttta acacacaatt accttccaca atggcatatt ccgccacctg cgaactgtaa
     7621 tgaatcgtcg gaatggcatc ttcttctatg ccagctttca cctgcgcgtg accgtagact
     7681 ttagcgcaat cgcatagcca gacgttgttc tcttcattac cttcaacact ggcaaaatca
     7741 aaaacttcgg cgcgatgttc aataaaagca taccggacga cggcatcgcc ataaatttgt
     7801 gcctggtgga caatacgcga ggcgctaacc cttgcgcgat cataaatttg gagcaggagt
     7861 tgatggtcgg gcgttaagcc ttgtgcggcg acgatcatag aatgttgatc aattaaggcg
     7921 tgaccaaata ttctacattg cacgtaaact aatgagtcat gaatggtgac actgtcactt
     7981 atatatgcac cctggctgat ttcactatta tcgatccaga cgttatccgt tgcgtaaacc
     8041 tctccccaca gcacgctggt gccggtgata cgcgtattac cggaaatcac cgttccgccg
     8101 aacgcaatag cattctggtc gtaaatccag caatttcctt cctgtgctaa caccgtttcg
     8161 cgatcgatcc agccgccagc ggttcctgct atcacatcgt taaaatcgct aatggcgata
     8221 atctgccgta ataacacact ttttttagtg ccatcttcct gataactaaa ggctcgctgt
     8281 tcttcactta ggcgatattt gcgcatagcg ttttcccaca ggtgacttac tataaccgta
     8341 gcaaattctg cggctctggc tatgctcgag aaaattccat aaaatgcatt tcaaatatac
     8401 tttataaatt aaacaaaatg agtaagaaga tgcagagcga taaagtgctc aatttgccgg
     8461 caggctactt tggtattgtg ttggggacga tagggatggg atttgcctgg cgctatgcca
     8521 gccaggtttg gcaggtcagc cactggttag gggatgggct ggtgattctg gcgatgatca
     8581 tctggggatt attgactagc gcatttatta cccgactcat acgctttccg catagcgtgc
     8641 tggcggaagt tcgccatcca gtgctgagca gttttgtgag tttgtttccg gcaacgacga
     8701 tgctggtggc gattggtttt gttccgtggt ttcgcccact ggcggtgtgc atgttcagtt
     8761 ttggtgtcgt ggttcagttg gcttatgccg cctggcaaac tgcgggatta tggcgcggat
     8821 ctcaccctga agaagctacc acgcctggac tgtatctgcc gacagttgcc aacaacttta
     8881 tcagcgcaat ggcctgtggt gcgttgggct acaccgacgc cggtctggtg tttttaggcg
     8941 caggcgtttt ctcatggcta agccttgaac cggtgatctt gcagcgtctg cgcagttcgg
     9001 gagaattacc cacggcactg cggacatcac tcggcattca gctcgctcct gcgctggtgg
     9061 cctgtagtgc ctggctgagc gtcaacggcg gcgagggtga cacgctggcg aaaatgcttt
     9121 tcggttatgg actgctgcaa ctgctgttta tgctacgtct gatgccatgg tatctctccc
     9181 agccatttaa tgcttcattc tggagtttct cgttcggcgt atctgcactg gcaaccaccg
     9241 gtttgcatct ggggagtggc agcgataatg gatttttcca tacgctggcg gtgccgctgt
     9301 ttatctttac caattttatt attgcaatac tgctcatccg tacttttgcg cttctgatgc
     9361 agggaaaatt gttagtcaga accgagcgcg ccgttttaat gaaagcagag gacaaagaat
     9421 gatcattcgt gacgaaaact attttactga taaatatgaa ttaacccgca cacactctga
     9481 agtactggaa gcggtgaaag tggttaaacc gggtaaaacg ctggatctgg gctgtggcaa
     9541 tggtcgtaac agtctttacc tggcagccaa tggttatgat gttgacgcat gggataaaaa
     9601 tgccatgagt atcgccaacg tcgagcgcat taaatccatt gaaaatctgg ataatttaca
     9661 cacccgggtc gttgatctga ataacctcac atttgatgga cagtacgatt ttattctttc
     9721 gactgtggtg ctgatgttcc ttgaggctaa aaccatccca gggttgattg ccaatatgca
     9781 acgttgcact aaacctggtg gttacaacct gattgtggcg gcgatggata ccgctgatta
     9841 tccatgtacc gtcggcttca cgtttgcctt caaagaggga gaattacgtc gatattacga
     9901 aggctgggag agggtgaaat acaatgaaga cgtcggcgag ctgcaccgca ccgacgccaa
     9961 cggtaatcgt attaaactgc gtttcgccac gatgctggca cgtaaaaaat gacccggtaa
    10021 gcacaaaacg cgtgaaaatt ccccacgctg agatgattta ctgttcttct tttcggtaag
    10081 catatttttt atcgaaggga tgtgaaatta atcacagtag tcgaagtttt ttagcagctt
    10141 aacttactga aatttaacta ctgatgattg acttagcccc tttttcggca ttgactatgt
    10201 cgtctgaaaa ggggctgaaa aatttatttt caccaacact ttttttgcca caacacgaag
    10261 cggcgctttt tgctataact tagaaagtaa tataatcatc tcaggaaact attcatgcgt
    10321 accacatcat ttgcgaaagt tgcagcttta tgcggcttgt tggctctgtc tggttgtgcc
    10381 tctaaaatca cccagccaga taaatataac aattactctg atttaaaaga aacaacctca
    10441 gcaacaggta aacctgtttt acgttgggta gacccgagtt ttgatcaaag caaatatgac
    10501 agcatcgtct ggaacccaat cacttattat ccggtaccaa aaccttctac ccaggtaggg
    10561 cagaaagttc tggataaaat tttgaactat accaacaccg aaatgaaaga agcgatagcg
    10621 cagcgtaaac cagtggttac caccgctggg ccgcgtagtc tgattttccg tggggccatt
    10681 accggtgtag ataccagcaa agaagggctg caattctatg aagtggttcc tgttgcatta
    10741 gtggttgcgg ggacgcaaat ggctacaggc caccgtacca tggatactcg cctctatttt
    10801 gaaggtgagc tgattgatgc agcgactaat aaaccggtta tcaaagtcgt tcgtcagggc
    10861 gaaggtaaag acctgaataa cgaaagtacg ccaatggcct tcgaaaatat taaacaagtt
    10921 attgatgaca tggcgaccga tgccaccatg tttgacgtta acaaaaagta gttccagacg
    10981 cgccatcgtt agatggcgct ttttatccgg tgcgccgtaa aaccccatcc ttcagggcgg
    11041 ggatataagg cgcggttttc cacctgacca ggtgtttgct gttgttcaat gtattggcgg
    11101 atgacggata ttggcgcacc tccgcagcta ctggcaaaat agccaggact ccacaaaacg
    11161 cctttgtagt aatacctgac tgcaatatct ggtcgatctc gtcgcagtaa cctacccgat
    11221 acgcctttga ggctgtttac cagactggat atcgccagtt tgggaggtta g
//
LOCUS       DAJTSA010000121        11110 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000121 DAJTSA010000000
VERSION     DAJTSA010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.121"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="OO369_003479"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="OO369_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR5983622.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="OO369_003480"
     tRNA            complement(621..695)
                     /locus_tag="OO369_003480"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="OO369_003481"
                     /pseudo
     CDS             complement(769..1701)
                     /locus_tag="OO369_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1995..2750
                     /gene="mlaA"
                     /locus_tag="OO369_003482"
     CDS             1995..2750
                     /gene="mlaA"
                     /locus_tag="OO369_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR5983623.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2932..3990)
                     /locus_tag="OO369_003483"
     CDS             complement(2932..3990)
                     /locus_tag="OO369_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983624.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
     gene            complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="OO369_003484"
     CDS             complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="OO369_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR5983625.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6068..6352
                     /locus_tag="OO369_003485"
     CDS             6068..6352
                     /locus_tag="OO369_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR5983626.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6532..7842
                     /gene="fadI"
                     /locus_tag="OO369_003486"
     CDS             6532..7842
                     /gene="fadI"
                     /locus_tag="OO369_003486"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR5983627.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7842..9986
                     /gene="fadJ"
                     /locus_tag="OO369_003487"
     CDS             7842..9986
                     /gene="fadJ"
                     /locus_tag="OO369_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR5983628.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            10189..10674
                     /gene="sixA"
                     /locus_tag="OO369_003488"
     CDS             10189..10674
                     /gene="sixA"
                     /locus_tag="OO369_003488"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR5983629.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2775 a         2544 c         2748 g         3043 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagccttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctaaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2761 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2821 aggagataaa ccgacaatga cccggacgta atgatgtaaa aatactgatg tttgtcactg
     2881 actatttccg atattcaggc gttgaaaaat caaaaaactg gaaaaataat tttaaatatc
     2941 atcaaaatta acacctagtg cagatagttg attaatttca ttttgatatt tcgtctggcg
     3001 attttgaagt cttttttcaa ctttatcttc gagattctta aagtctttgt atttattttc
     3061 aggatggtca acgtcaactt tgttagcgaa taaagtagaa attatttctt ttacattatc
     3121 gcgtctaaat tttgcatcgt ccacttgaaa attatatctt attgctcgac ctaaacgttt
     3181 aaaaacttcg ttaggttctc tcagtctttc cccgttgagc tcgtttacaa cctgatgatc
     3241 ggaaccagtt gcaaatgatt ctgccatgat agctttactg ctttcaaacc acgctgaagc
     3301 actcctggcg ttttcactac aatattgggt gagactatca ccattaatac gaattcctct
     3361 ttgaatgtgg tagtcgtcct gatcgtagta accgagagat aagtaatcat ttccgttttt
     3421 atttttaatt acaatagcga gagagtcgcc ttttttatct ttaccgtacc aggtaaataa
     3481 gtccgttggt tcgcttttcc cgctctcatc aaattccgtt accgtatgtc tgagtttgtg
     3541 tgatatctta tcgaagataa aaccaggatc tccatcatat atttctttta atgcgctgaa
     3601 gatcatttcc ttgttattga tatcatgagt cttctcaact atgttgtcat taatgatagt
     3661 acggacgagt tctttgtctt tatagttcgc aggtaattct attggttctt ggttgtcgat
     3721 accaatagta atatggtctg tatcattgga gccaatatca atataagtaa ctgaggaatt
     3781 attaatatta gatctataag gatcgtcgtt tactcgtatg gcgtttttta taagctcaag
     3841 tagatgttta atacaggtgc ttattccttc cttaccatta aaaaggtttt tgaactcttg
     3901 tactaatcca ttaatttcat cattgttatt ttcattgatg gattctgggt aagctgcgct
     3961 ggtattgttg attgaaatag atggtagcat gattttattc catgttgatt gatttgttac
     4021 aaatatatat tacctaatga aatagactat caaaaaacgc tcatttattt agaacctatc
     4081 tttaatttga gtgtataagc gcacaatagc caggggggtt gagtatcctg gaaatgcaga
     4141 aaagtaaact ctttgagaag gacgagcaga acgcttgaaa atatgcattt cccttcaggt
     4201 ttgtctgtgg ataccactta ctgatttgaa agggcaagct gccatcaggc tgtttgcaaa
     4261 actcaggagg caagcaatga ggaaactact ttcggtgaag tgggggagtt aaagtcacct
     4321 gcatagcagg tgactttatc caggcgaacg cgttatcaga acgcgtagtt aaagttagta
     4381 ccgaacagcc aggctttacc ttcagactcg aactggtatg ggccttcgtt aattttcacg
     4441 ctctgaccgt gcatataaga aacaccaacg tcgactgaag catctttatt aaatgcgtaa
     4501 gtcgtacctg cactcagcca gaaacggtcc tggtccggaa tggagataga acgattctga
     4561 gccggaactg ggctgtcatc aaaggcgata ccggtacgga aggtccagtt atcatcgtag
     4621 taataagtgg tacccaacgc gatgcggtaa gcatctttaa agccttcatg tttctggaac
     4681 agcgtgtcgc cactggttga ggtcgctttc agctgctgga actgactcca gctggtgtaa
     4741 gccaggctat agtgaatcgc ccactgtgga tcaacacggt tataacctga cacttcccac
     4801 atttcaggca ggttcagcgt cagataaccc gattgcgttg cgccacctgt cgcggtagga
     4861 attggcaaac cgtagttatt aaacgcacga ttaagatcgc tgctgtagtt acctttgaag
     4921 tcaattttca cttcagaacg gtaggtcagt gcatagcggt tatttttatc cagttcatac
     4981 aggattccgg cgttccagcc aaagccccac tggttaccgt tcagatgagc gattttggta
     5041 ttactgtcga taccgttggc ggtagctgcc aatgcttgcc cttgaggagt ttgcccggca
     5101 ggagattgca taatctgacc agcaaccaac tgccccagat cgcctgcgaa acgttcaatt
     5161 ttcgcgcgag cgtagatggc gttgaaacca agaccaaagc tccatgcatt atttaagcga
     5221 tacgcaccgc ttaagttcag gttcatggtt tcaaggtcag ttgtaccccc gacagagccg
     5281 cctgcataag tatcattaaa ctctgttgcc agaccatagt tagaggtaat agaagcgccc
     5341 caaccaaatt ggtcgttaat cggtgcaaca aagtgcatgt tcggaaccca tgccgtaggc
     5401 gcgatgttat cggctttcag gctacgacca gacggagacg ttccgctgat attcacatcc
     5461 gggtcaatat aaaccgcacc cgcagaaaat gtcgggcggt caaacatggt aatcaatgcg
     5521 gggttacggc taacgttacc tgcatcatcg gcaattgcgc cttcccctga ataagcccgg
     5581 cccaggccag aggaagaaaa ttcgtttaac tgaaagcctg ccgaccaggc ctgggtggag
     5641 ataagtgcca ctgcgactgc gagagcagac tttgtaaaca gggttttctg gctcatgacc
     5701 ataacctcaa tgatttattt ttatacaaac tatgttacgt gctgtaacag gagcgcgaag
     5761 tgtagggtct gaggtacatc ttagaaatca gaccagtggc gagagtatag gtcggaccag
     5821 ctagaatgtt gcaagtatgt ttccatcttt tttcaaatat gatctaagaa acgcgatcca
     5881 gctggcaaaa tttggaggtt gcgcactcgc ggattcgggt gaattttgtt ttagatcatt
     5941 tttaagtgtg atttcggtca cttatccgat tttgcagaat taacaactgg agcagcactt
     6001 tccggaagcg taaaatatcc acaacgttta tctggcacct tgggtgcaca tacagaggaa
     6061 atcaacgatg agtaaatgta gtgctgatga aaccccggtt tgctgctgta tggatgttgg
     6121 caccattatg gacaactccg actgcaccgc gtcttacagc cgcgtgtttg caaaccgcgc
     6181 agaagcagaa caaacgctgg cggcgctgac tgaaaaatcc cgtagcgtgg aatccgaacc
     6241 gtgcaaaatc tccccgactt tcactgaaga gtctgacggt gtgcgcctgg atatcgattt
     6301 cacttttgcc tgcgaagctg aaatgctgat cttccagctg ggtctgcgtt aattcctctc
     6361 gggtacgtac gcccctgtca gttgctggca ggggcgtttt cttttctttc ttctgtcttc
     6421 actgtgtcat agctcccact ttcctttttc tccgcttggc taaatgtaaa aaaatggtta
     6481 agactatgat caggtcagac cactttattt atttttttac aggggagtgt tatgggtcag
     6541 gttttaccac tggttacccg ccagggcgat cgtatcgcca ttgttagcgg tttacgtacg
     6601 ccttttgccc gccaggcgac ggcttttcat ggcattccca cggttgattt agggaagatg
     6661 gtggtaggcg aactgctggc acgcagcgag atccccgctg aagtgattga acagctggtc
     6721 tttggtcagg tcgtacaaat gcctgaagcc cccaacattg cgcgtgaaat tgttctcggt
     6781 accggaatga atgtgcatac cgatgcttac agcgtcagcc gcgcttgcgc taccagtttc
     6841 caagcggttg caaacgtcgc agaaagcctg atggcgggaa ctatccgagc ggggattgcc
     6901 ggtggggcag attcctcttc cgtattgcca attggcgtca gtaaaaaact ggcgtacgtg
     6961 ctggttgatg tcaacaaagc tcgaaccatg agccagcgcc tgaaactctt ctctcgcctg
     7021 cgtttgcgcg acttaatgcc cgtaccgcct gcggtagcag aatattctac tggcttgcgg
     7081 atgggcgaca ccgccgaaca aatggcgaaa acctacggca tcacccgaga acagcaagat
     7141 gcattagcgc accgttcgca tcagcgtgcc gctcaggcat ggtcagacgg aaaactcaaa
     7201 gaagaggtga tgactgcctt tatccctcct tataaacaac cgcttgtcga agacaacaat
     7261 attcgcggta attcctcgct tgccgattac gcaaagctgc gcccggcgtt tgatcgcaaa
     7321 cacggaacgg taacggcggc aaacagtacg ccgctgaccg atggcgcggc agcggtgatc
     7381 ctgatgactg aatcccgggc gaaagaatta gggctggtgc cgctggggta tctgcgcagc
     7441 tacgcattta ctgcgattga tgtctggcag gacatgttgc tcggtccagc ctggtcaaca
     7501 ccgctggcgc tggaacgtgc cggtttgacg atgagcgaac tgacattgat cgatatgcac
     7561 gaagcctttg cagctcagac actggcgaat attcagttgc tgggtagtga acgttttgct
     7621 cgtgaagtac tggggcgtgc acatgccact ggcgaagtgg acgatagcaa atttaacgtg
     7681 cttggcggtt cgattgctta cggacatccc ttcgcggcga ccggcgcgcg gatgattacc
     7741 cagacattgc atgaacttcg ccgtcgcggc ggtggatttg gtttagttac cgcctgtgct
     7801 gccggtgggc ttggcgcggc aatggttctg gaggcggaat aatggaaatg gcatcagcgt
     7861 ttactcttaa tgttcgtctg gacaacattg ctatcatcac catcgacgta ccggatgaga
     7921 aaatgaatac cctgaaggcg gaatttgcct cgcaggtgcg cgccattatt aagcaactcc
     7981 gtgaaaacaa agagttgcgc ggcgtggtgt ttatctccgc gaaaccggac aattttatcg
     8041 ccggagcaga catcaacatg attggcaact gcaaaacggc gcaagaagcg gaagcactgg
     8101 cgcggcaggg ccaacagttg atggcggaga ttcatgcttt gcccattccg gttatcgcgg
     8161 ctattcatgg cgcttgcctg ggcggtgggc tggagctggc gctggcgtgc cacggtcgcg
     8221 tttgtactga cgatcctaaa acggtgctcg gtttgcctga agtacaactt ggattgttac
     8281 ccggttcagg cggcacccag cgtttaccgc gtctgatagg cgtcagtaca gcattagaga
     8341 tgattctcac cggaaaacaa cttcgggcga aacaggcatt aaagctgggg ctggtggatg
     8401 acgttgttcc gcactccatt ttgctggaag ccgctgttga gctggcgaag aaggatcgcc
     8461 catcttcccg ccctctacct gtacgcgagc gtattctggc ggggccgtta ggtcgtgcgc
     8521 tgctgttcaa aatggtcggc aagaaaacag aacacaaaac tcaaggcaat tatccggcga
     8581 cagaacgcat cctggaggtt gttgaaacgg gattagcgca gggcaccagc agcggctatg
     8641 acgctgaagc tcgcgccttt ggcgaactgg cgatgacgcc gcaatcgcaa gctttgcgta
     8701 gtatcttttt tgccagtacg gacgtgaaga aagatcccgg tagtgatgca ccgcctgcgc
     8761 cattaaacag cgtgggaatt ttaggtggtg gcttgatggg cggcggtatt gcttatgtca
     8821 ctgcttgtaa agcggggctt ccggtcagaa ttaaagatat caacccgcag ggcataaatc
     8881 atgcgctgaa gtacagttgg gatcagctgg agggcaaagt tcgccgtcgt catctcaaag
     8941 ccagcgaacg tgacaaacag ctggcattaa tctccggaac gacggactat cgcggctttg
     9001 cccatcgcga tctgattatt gaagcggtgt ttgaaaatct cgaattaaaa caacagatgg
     9061 tggcggaagt ggagcaaaat tgcgccgctc ataccatttt tgcttcgaac acgtcatctt
     9121 taccgattgg tgatatcgcc gctcacgccg cgcgacctga gcaagttatt ggcctgcatt
     9181 tcttcagtcc ggtggaaaaa atgccgctgg tagagattat tcctcatgcg ggtacatcgg
     9241 cgcaaaccat cgccactacg gtaaaactgg cgaaaaaaca gggcaaaacg cccattgtcg
     9301 tgcgtgacaa agccgggttt tacgtcaatc gcatcttagc gccttacatt aatgaagcta
     9361 tccgcatgct gaccgaaggt gaacgggtag agcacattga tgccgcgcta gtgaaatttg
     9421 gttttccggt aggcccaatc caacttttgg atgaggtagg aatcgacacc gggactaaaa
     9481 ttattcctgt actggaagcc gcttatggag aacgttttag cgcgcctgca aatgttgttt
     9541 cttcaatttt gaacgacgat cgcaaaggca gaaaaaatgg tcggggtttc tatctttatg
     9601 gtcagaaagg gcgtaaaagc aaaaaacagg tcgatcctgc catttacccg ctcattggcg
     9661 cacaagggca ggggcgactc tccgcaccgc aggttgctga acggtgtgtg atgttgatgc
     9721 tgaatgaagc agtacgttgt gttgatgagc aggttatccg tagcgtgcgt gacggggata
     9781 ttggtgcggt atttggcatt ggttttccgc catttctcgg tggaccgttc cgctatatcg
     9841 attctctcgg cgcgggcgaa gtggttgcaa taatgcaacg actggccacg cagtatggtt
     9901 cccgttttac cccttgcgag cgtttggtcg agatgggcgc gcgtggggaa agtttttgga
     9961 aaacaactgc aactgacctg caataagaag gtcaaagcta tatgaatccg cgctgaatgg
    10021 cggagtgttg gtcaaaatgt aaacgcatat tgactatact tacgccattg aggtaaaaaa
    10081 cagcgtttca ttcggtgaat ggataaggca caatgccggc caccgttttc tttctctggt
    10141 ttcagatgaa agaaaacggg cgaatctggt taacaaaagc ggtgcaatat gcaagttttt
    10201 atcatgcgtc acggcgacgc agccctcgat tccgccagtg attcggttcg tcctctgacc
    10261 actaatggtt gtgacgaatc tcgcctgatg gcgaactggc tgaaaggtca aaaagtggaa
    10321 atcgaacgcg ttctggtgag cccgttcctg cgagccgagc aaacactgga agaagtaggg
    10381 gattgtctga acctgccttc cagtgcagaa gttctgccgg agttaacgcc ctgcggcgat
    10441 gttggtctgg tcagtgccta tttgcaggcg ttgaccaatg aaggtgttgc ttcggtactg
    10501 gtgatctctc acttaccgtt agtcgggtat ctggttgccg agttatgtcc gggcgaaacg
    10561 ccgcctatgt ttaccacctc ggccattgcc agcgtaacgc ttgatgaaag cggaaacggt
    10621 acattcaact ggcagatgag cccgtgtaat ctgaagatgg caaaagctat ctgatgtgtt
    10681 ccaaattatg cccaatgagg tagattcatt gggcataatt gctcattagg cagcggatat
    10741 ctgtcaacct cactactatc gaagactctc cttatctata aataaaacac tttctacatt
    10801 cattcggaaa ttattcttct acgcgtatct tgattttgcc caaagttaca tgttgaaatg
    10861 aataatatct gtttgacact cattgtcatt tattaataca gataatagag aaaatagagt
    10921 tgaataatca ggcaggatgt aattgaaact tgattaaatt ctggctatag tgtttatata
    10981 ttatattgtc aaaagtggat aaatgccaat cgcattatat gttcattgtt tgttttttaa
    11041 gctgttgata ttcattattg atatactttc tattttttct aaatacttat tccgtatttt
    11101 ttaaggatgt
//
LOCUS       DAJTSA010000122        11046 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000122 DAJTSA010000000
VERSION     DAJTSA010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11046
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.122"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            409..1239
                     /locus_tag="OO369_003489"
     CDS             409..1239
                     /locus_tag="OO369_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5983630.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="OO369_003490"
     CDS             complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="OO369_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR5983631.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2368..3828)
                     /locus_tag="OO369_003491"
     CDS             complement(2368..3828)
                     /locus_tag="OO369_003491"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR5983632.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="OO369_003492"
     CDS             complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="OO369_003492"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR5983633.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5433..6776
                     /gene="gntP"
                     /locus_tag="OO369_003493"
     CDS             5433..6776
                     /gene="gntP"
                     /locus_tag="OO369_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR5983634.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="OO369_003494"
     CDS             complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="OO369_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR5983635.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="OO369_003495"
     CDS             complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="OO369_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR5983636.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="OO369_003496"
     CDS             complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="OO369_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR5983637.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(9005..>11046)
                     /gene="fimD"
                     /locus_tag="OO369_003497"
     CDS             complement(9005..>11046)
                     /gene="fimD"
                     /locus_tag="OO369_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR5983638.1"
                     /translation="IGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKW
                     QHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVI
                     HGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFT
                     VPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLA
                     DRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQ
                     LVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTV
                     TQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRD
                     QMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSY
                     SVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANG
                     VTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNTL
                     ADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQSS
                     GIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2684 a         2828 c         2682 g         2852 t
ORIGIN      
        1 acgtagatga aaatacttgt caccgctgct acttaattcc ggtgggaaat tatttatttc
       61 cgcataatca gctatgtatt atttgaaaca attagtttgt aaataatata aattgataac
      121 atatcaaata catcaaaaat accattcaca ggcattatta atatagacct caatatagca
      181 atatcaaatt ccaattagta ataagatatg cttttaaatt gcatgcaaat aattttaaat
      241 gagcgttttt tgatacttta gagtgtttcc taccctttaa tatcactgtg tagagttgat
      301 atcactacaa cagaaacatg catgtcattt gtttacttct ctttttacta cagaaaaatt
      361 tatctatttt tctaaacatc agttaaatac tcataaaagg taactattat gtctatgcca
      421 ttaagcaatg ccgctaataa ccaattcaat acaaataatc acttccttca tcatccaaaa
      481 gtcgacagtg agttaacacg tcgatatgaa tatgcacgcc tggatacgga aaatatctat
      541 cttctgcctc ttgctcgtgg caataaccat aattacgatg gaaaaagcgt tgtcgaaatt
      601 agaaaactta atattggcaa acagtcctgg ccatttaatt atgtgacaga tacatgcaga
      661 gaattcaatg gtattacaac aacaggacgt atgctctatc gaaacttgaa aatcacctca
      721 gctttagacg aaatatatgg tggtatatgt aaaaaggcgc atgctgctac agagttagca
      781 gaaggtttac ggctaaattt atttatgaag agccctttcg atcctgttga ggactataca
      841 gttcatgaaa taacattagg ccctggttgc aatgttcctg gttatgccgg aaccacgata
      901 ggatatattt caacccttcc agcttctcag gcaaaaagat ggaccaatga gcagccaaga
      961 attgatatct atattgatca gataataact gtctcagggg tagcaaattc ttccggattc
     1021 gcccttgccg cattacttaa tgctaatatt gagatgggaa atgacccaat cattggtata
     1081 gaagcctatc ctggtaccgc ggagatccat tcaaaaatgg gatataacgt tatccccggt
     1141 gatgaagatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc agaactgttt
     1201 gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact
     1261 aatattcagg ttatacagat atttaaacaa aaccctgcaa tttacaaagt aatactcatt
     1321 gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt
     1381 caggcgtcga ctttatccag cggccatgaa tcaaagagat agccgtcaaa ataaatatcg
     1441 tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc
     1501 agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc
     1561 aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga
     1621 ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct gttatgcgtt
     1681 gcttcagcaa tggcgagatg gaactgcatg tcaccgcttt cgctgctacc cggcgcgctg
     1741 gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg
     1801 gtagcctgca gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg cgcctgtaac
     1861 agctcaaaag gacccgcatc attgcagacg ttggcatccg gactgtctgt gttgtggctg
     1921 cctgagctat caagcacata gataccggca ccccggcgca cttccaccag ccctttgatc
     1981 tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt
     2041 tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgtgg
     2101 atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tgacagattt catggtgttg
     2161 tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc
     2221 ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag cactggaagt
     2281 tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac
     2341 cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg
     2401 ttcgcacagc tgctgatatg ccgacgtcac cgcgccaaca aagtcggcat tctgcggcag
     2461 atcatcggca aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata
     2521 ctgcgcgtta atcttctgga actcggccag catcgggtcg accacgtcaa tggcattacc
     2581 ctgctcatcc acgccctggg tgtaacccat ccagccagcc acgcccagcg ccaggtgacg
     2641 ccagctgccg ccgttttgca ggtgcagacg caccgggtcc agtagacgct gcggtaactt
     2701 ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg
     2761 ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg
     2821 cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga taatccgggt tagtcatggt
     2881 gtcggcaatg gtttcatagc cgccgaggta accgaggtac gccaggaaag agtggctgcc
     2941 gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgtgcc
     3001 cactttatcc cagtccgggc gaccattaac gaagttatct tcaatcaccc actgacggaa
     3061 cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa tttcctgtaa
     3121 ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt
     3181 ctcttcaatc catgccgcta gctgcgggtc acgcgcctga gccagcccca gtaccgcgac
     3241 cttcgccaca tggccgtttt cacgcacgtt atcgcaggac atcaccgtaa atgctttcag
     3301 ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc
     3361 agtcgggttt tccagatcgt gcttgatcag cgggttattg agatccagtt gaccgcttgc
     3421 cgcatcagcg cagtagcctt tttccgtgac cgttagagag acaatcgccg tttgcggacg
     3481 cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg cttctttcat
     3541 cgaaccgata attttcagct cggtgctttc tgcgcctttt tccgctacgg tgtacagcag
     3601 ttgctgtttt ttcaggtttt cgatcagcat gcggtcgttg cctggcatca ggttaacttc
     3661 gcagatgccc cagtcgctgt cggtgctttc cagcagatga tgggtataca gcgcctggtg
     3721 cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgatc
     3781 ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag
     3841 aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg cagccatgcc
     3901 ggtgggtatt aacggctaaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg
     3961 gccaggcctt tcagacgacc aattgcggag taaccagggt tggttttctt cttcaggtcg
     4021 tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct
     4081 ttacgacggt gttcttcttc aacaatcgct ttcaccactt tgtacatatc aacgtcaccg
     4141 ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga
     4201 tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca
     4261 cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt tacggtatca
     4321 accatccact gcatatcttc aatggtggaa acaatgcgcg gcaagccgag gatcgggcgc
     4381 ggcggatcgt ccgggtgaac cgccatacgc acgccaactt cttcagcaac tggaataatc
     4441 gctttcagga agacggcaaa gttttcgcgc agtttggctt tgtcgatatc tttgtacagc
     4501 tccaggtgct tacggaactg gtcgagggta tacccttctt ccgcgcccgg cagaccggca
     4561 atgatgttac gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct
     4621 gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt caggatatgc
     4681 atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc
     4741 acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg
     4801 cgaatgccac actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca
     4861 tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac
     4921 accaggcctg cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact
     4981 tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca
     5041 tcagctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt
     5101 cctctttcac atagtgggtt ccacagcaaa gagacgcgga accttggagt tgtttaggta
     5161 tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt
     5221 ctaccttttg gtaagacatg tcaaccagaa ttctgaaatt ggttaaccgc gtcgcaagaa
     5281 tttcactctc atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca
     5341 gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt cgcccaaaat
     5401 aacaacggtt caaccaagta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg
     5461 tattcggcat tggcctgatg ctgatactga atttgaagtt caaaatcaat tcaatggtgg
     5521 ctttgttggt ggcggcgctg tccgtcggga tgctggcagg catggatttg atgtcgctgc
     5581 tgcacaccat gaaagcgggc ttcggcaaca cgctgggaga actggctatc atcgtggtgt
     5641 tcggtgcggt catcggtaaa ttgatggtcg actccggcgc ggctcaccag atagcacata
     5701 cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc ggtgattatc atcggcctta
     5761 ttttcggtct ggcgatgttc tatgaagtgg cctttatcat gttagcgccg ctggttattg
     5821 ttattgccgc cgaagctaaa attccgttcc tgaaactggc gatcccggca gtagcagctg
     5881 ccactaccgc acattcactg ttcccaccgc agccgggtcc ggtggcgctg gtgaatgctt
     5941 atggcgcgga tatggggatg gtttatatct atggcgtact ggtgacgatc ccaagtgtaa
     6001 tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct tgagcgccca acgccatcat
     6061 tcctgaaagc agatcaaccg gtagatatga ataatctgcc ctctttcggc gtttcgattc
     6121 tggtgccgct gatcccggcg atcattatga tctctaccac catcgccaat atctggctgg
     6181 taaaagatac ccctgcctgg gaagtggtta actttattgg ttcctcgccg attgcgatgt
     6241 ttattgcgat ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat gacatgcagt
     6301 gggtgatgaa cgcttttgaa agcgcggtga agagtattgc aatggtgatt ctgatcatcg
     6361 gtgcgggtgg cgtgctgaag cagaccatca tcgacaccgg cattggcgac accatcggca
     6421 tgttgatgtc ccacggcaat atctcgccct acattatggc atggctgatc actgtgctaa
     6481 ttcgtctggc gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg attatcagtg
     6541 ctgcaatcct tgatccagca accggtcagc tggttggcgt gaatccggcg ctgctggtac
     6601 tggcgacggc tgcgggttcc aacaccctca cccacattaa tgatgcatct ttctggttgt
     6661 tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa aacctggggt ctgctggagc
     6721 tggtcaactc cgtggttggg ctgattattg tgctgattat tagcatggta gcgtaaaaga
     6781 acaccagagc ctgccaatgg caggctcaga ctgatgacta aagtctaaaa atgtgcccgg
     6841 acagtcccct cgcccctccg gggagagggt tagggtgagg ggaacagacc agtaaagtgc
     6901 gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt
     6961 gcaggttttg taggtctgat agcgtagcgc ctcaggtacc agcattagca atgcgctgta
     7021 atttttttat tgataaacaa aagtcacgcc aataatcgat tgcacattcc ctgcagtcac
     7081 ctgccctccg gtacgtgcgt aatttgccgt taatcccaga cttaccgccg aagttcctac
     7141 tgctcctaac gataccgtgt tattcgctgg aataatcgta ccgttgcgcg tcaactgtac
     7201 gccgacgccc tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc
     7261 tgcggttgtg ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag
     7321 aggaattggc actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac
     7381 atcacaaccg ccagtgggca ccaccacatc attattggcg taaatattcc acacaaactg
     7441 aaaatcatcg ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgaacc
     7501 agctttaata gccaccccgc ccgcactgct cacaggcgtc aaataaagcg ccaccggcca
     7561 cggcttatcc gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg
     7621 atagctactg ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccgtaagc
     7681 cgagcctcgt tgcagtgtga catagtctgt aatggtttcc ggataatcgt tatggcaaaa
     7741 gatttgcgtc gaaagatcta cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt
     7801 tacataaaca ttagcgctgc caccgccaat agggatagcg gtaccattgg cggttttaca
     7861 ggcgaatgac caggcattta ccgaccagcc catcagcagt acagcaaaca gggtaatagc
     7921 tcgtttcatt acaattatct cttcgggttc agctgtaggt ataggtgatg ctaatcactg
     7981 cctgaatggt tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga
     8041 agtgcgctga ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca gtattcaatg
     8101 tgttgccact gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat
     8161 aatatccggt gctgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg
     8221 gacaattagt caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacataa
     8281 gactgaaaga ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa accgtacacg
     8341 gtttggcgac gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac
     8401 ttgcgagcgc caacattgcc gccaatacat acccacgttt gcaccatttc atgagcatct
     8461 ccagttactg atattcaaga gtgaaggtag ccgtagcatt gatatgcccc gcagtgacag
     8521 gcacctgtgt cgccattagc cgggcgtaaa aattcagcgt atttggttta cccggcgtca
     8581 gggtcgtcca cgaaatcgca gacgatggag cattaagggg tatttgattt tgctgctcat
     8641 tcagaagctg tattcccagt cccgcagccg ctgacaccgt attttcaagt gcaagcaggt
     8701 tggcattgtg gctatctgca acgccggtaa acccaacttt tacggcagaa acggcattac
     8761 cacagggtga cagcaaaata cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa
     8821 attgcttcgc cgcgttttcc atcagatcaa cagtaaaatt ggttgattca gcggccacac
     8881 tacagccgtt atctctgaca tagccgcgga tagtaatcgt gctatccgca gccaaaacgc
     8941 gacttaccgc cagccacaaa aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc
     9001 ccccttaacg acattcagct gatagctggg ttaataactg ctgctgactc tctggtggca
     9061 gttgataatt ggcgacacag tgagcatttt cctcttctcc ccatttcacc tgaacttttc
     9121 ccgctaaagg cattccgctg aggtaaacct gaccattatc cgcaacaatg ccgctactct
     9181 ggctactctc tgatgtcacc atcgccccaa acggcagcgg cttattattg tgagttagcg
     9241 tcatgagcag ttttatccca acgcgcgctt taaactctgc tcgcacgatc gccccacgag
     9301 tgggaacaac gttagcgacc gcgttatcta aatcgacgtt atcagccaag gtattggtat
     9361 ccagcgccac tctattttcc cgatattcag tggcataagg cagcacggca taaccacgcc
     9421 agtcggtacg cacccccgtc tggttttcga cttttgcatc ttttgcgcca ggcgctttaa
     9481 caagcaccac cgtatcgttt aacggctgcc ccagcgttac gccattggca tgagccaata
     9541 ccccaccgct gactccgtaa tagagctgct taatatcatc gctatggctg taaccgatat
     9601 tggcattgcc gtaaccaccg cgataattca gcgtggcgta gcctgtactt ccgctattac
     9661 catcgcctcc cccggcatag ccggtttgca cgctataact gaggttgttg tcttccagca
     9721 acgtaccgta tacaccagcc agattggtca tccgaccgtt gagatcgtgt gacatgctgt
     9781 agctggcact ggcatgtcgc cactgagatt tactgtcaga acgcagccag tggctgaaag
     9841 gaatattgac gttaagcgct aacatctgat cccgtccttt ttgccaggcg tttttcgtca
     9901 ggctatagct gagcgtccag ttgatatctt cgaacgcagt atttaatcca gcctggaatt
     9961 gctcatcgac attactcgtt ccccaataag tttgatggct accactcaaa tacagtgttg
    10021 atgtgcgccc gagttgctga gtaacggtga gctgtaattt cccgcgtttg ttataagcga
    10081 ggttgtaata gtcggtgaat ttcggcttaa cctgaataac tccatcctgt gtttcgatgt
    10141 tgtagccatt cattcgactg tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat
    10201 aacggtaacc cactaactga atattcgtgc ctgattcatt gagcgatttg ttatagagaa
    10261 aacgcaccga ttgtccgtca tgctgactgt catcgggaag tgtggaatta gcctgcgtca
    10321 tatccacaga cagagcgccc agtgcctcca tgtttttccc gataccgaaa ttaaaagcac
    10381 gataacgatc cgccagttgc gttccaccat atattgtcca gccagccgga aggccgtgga
    10441 gtaatgtact ctggaaaaag cggggttttt cctgctgcgc atttccacta cggtattctc
    10501 ctgccgtaat ggaataacga gtatgccctt cacgttgcaa aagcgggact gacgaatagg
    10561 gtacggtaaa aatctgcgtg ctgccgtcag cctctttgat cgttacctgc aagtcaccac
    10621 tattacctgc ggcatagata tcgttgatgg taaaaggccc cggtggcacc gtactattat
    10681 aaatgccata tccattttgt ttaatagtga cctgtgcagt accacgagca ataccgtgga
    10741 tcaccggggc aaatcctctt tggctatcgg gtaacatatt gtcatctgag gccaattgtg
    10801 cgccgcgaaa gttaatacca tcgaaaatat cgccctgggt ataaccatca cccaacgtca
    10861 gccgggaacg taaaggaatt atgtctcgct caatccaggt aatgatatgc tgccatttat
    10921 ttttgctacc tgatgaactg tcgctactgt tataactcca ggtggtattg tcgcgtaaac
    10981 gccacgcacc aatatttaac ccactctgta ggtttaaata tgcataatgg ctgttacccc
    11041 caatcc
//
LOCUS       DAJTSA010000123        11036 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000123 DAJTSA010000000
VERSION     DAJTSA010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11036
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.123"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            154..504
                     /locus_tag="OO369_003498"
     CDS             154..504
                     /locus_tag="OO369_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR5983639.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            557..952
                     /locus_tag="OO369_003499"
     CDS             557..952
                     /locus_tag="OO369_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR5983640.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            993..1736
                     /gene="cmoA"
                     /locus_tag="OO369_003500"
     CDS             993..1736
                     /gene="cmoA"
                     /locus_tag="OO369_003500"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR5983641.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1733..2704
                     /gene="cmoB"
                     /locus_tag="OO369_003501"
     CDS             1733..2704
                     /gene="cmoB"
                     /locus_tag="OO369_003501"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR5983642.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="OO369_003502"
     CDS             complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="OO369_003502"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR5983643.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5323..6423)
                     /locus_tag="OO369_003503"
     CDS             complement(5323..6423)
                     /locus_tag="OO369_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR5983644.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="OO369_003504"
     CDS             complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="OO369_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR5983645.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7571..8137)
                     /locus_tag="OO369_003505"
     CDS             complement(7571..8137)
                     /locus_tag="OO369_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5983646.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8353..10086
                     /gene="argS"
                     /locus_tag="OO369_003506"
     CDS             8353..10086
                     /gene="argS"
                     /locus_tag="OO369_003506"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR5983647.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10263..10751
                     /locus_tag="OO369_003507"
     CDS             10263..10751
                     /locus_tag="OO369_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCR5983648.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(10869..10937)
                     /locus_tag="OO369_003508"
                     /pseudo
     CDS             complement(10869..10937)
                     /locus_tag="OO369_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
BASE COUNT         2689 a         2815 c         2635 g         2897 t
ORIGIN      
        1 taatgggctt ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact
       61 catagctaac cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta
      121 ggaaccatct gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt
      181 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
      241 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
      301 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
      361 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
      421 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
      481 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
      541 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
      601 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
      661 tggttttagc gaactgcaaa gcgctatttg cattcatggt aacgcggtgg aatatattcc
      721 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
      781 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
      841 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
      901 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
      961 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     1021 cccctatcgc cagactgggc gactggacct ttgatgagcg ggtcgctgaa gtcttcccgg
     1081 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     1141 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     1201 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     1261 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     1321 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     1381 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     1441 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     1501 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     1561 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     1621 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     1681 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     1741 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     1801 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     1861 caacacggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     1921 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     1981 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     2041 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     2101 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     2161 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     2221 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     2281 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     2341 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
     2401 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     2461 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     2521 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     2581 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     2641 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     2701 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     2761 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     2821 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     2881 agcaggctta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     2941 cagcgcagag ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa
     3001 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     3061 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     3121 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     3181 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata
     3241 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     3301 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     3361 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     3421 cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc
     3481 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     3541 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     3601 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     3661 ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
     3721 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     3781 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     3841 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     3901 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
     3961 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     4021 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     4081 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     4141 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     4201 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     4261 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     4321 ttccgccgtc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     4381 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     4441 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     4501 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     4561 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     4621 gctccaggca atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct
     4681 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     4741 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     4801 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     4861 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     4921 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     4981 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     5041 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     5101 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     5161 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     5221 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     5281 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     5341 atctttggcg ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct
     5401 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     5461 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     5521 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     5581 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     5641 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     5701 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     5761 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     5821 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     5881 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     5941 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     6001 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     6061 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     6121 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     6181 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     6241 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     6301 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     6361 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     6421 catagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     6481 gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga
     6541 tttattacaa tgtaatcatt aattgctaag gaataaccca gttgctattt agaatatata
     6601 tttatcagtt ttagcaattt aaatcccata atcaatgtga atataaaaaa tatttttagc
     6661 acaacgctat gcgcactgtc acccgctcaa cagatcacgg ttatcgttcg ttttttatac
     6721 tgctcaggga taaaaaaagg cccctgttga aattgcaggg gcctggtacg agcaagcatc
     6781 atattgggcg acatgatgca acggtaaaaa tcatttggcc tgatggcgtt caatgattcc
     6841 tttcatttca gcaaccgccg ccccgtctac gatataacgc gaatactcgt ccgcgtgttc
     6901 atctgatgac atggacaatc cttgattacg ataacgcatc ggtgaggctt gccacgctcc
     6961 cgcggagcta tggacttcca gcactccggc atcgaggaag tggtgcaggt tttctgcacg
     7021 gactcctgct ccagccataa tgattggagc atcacgatgg gcaataagtt ccataatttt
     7081 tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc agtacccggg caatgcccaa
     7141 ttccgcaaga ttattgagtg tatataaagg gttagcgcac atatcgaagg cgcgatgaaa
     7201 agtcactgcc agcggaccgg cagcagccat tattttttcc attcgtggca tatcgacatt
     7261 cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa cctaattcgc ggaccgtgcg
     7321 cacatcctca agaatggcgg caaactcacc gtcgctgtag caaaaatcac caccgcgtgg
     7381 gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc acggatttca gtacccccaa
     7441 cgacggcgtt aagcccccct cttttggggc tgcgcataat tcaactctgt ctgcgccgtt
     7501 ttgctgcgcc gttagtgcac attccatgct gtaacagcaa atttccagta atgccatttt
     7561 tgctccttaa ttacgctgac tgctcgctgg caacgatctc ttcaatggac cacggatgaa
     7621 acttaatagt tgttttgcca tcggtgaccg ccagcgtcgg attcggtaat cgctcatgct
     7681 caccttttgg tgaactggtt ttaactgaga tccccggtag gctgagtcct tcatcagaaa
     7741 gcaatgccag cgcacgggca ttcagcgttt ctggatcacc cggcagaacg atttcaatat
     7801 gttcccaacc ttcgtgtggg taacgttttt ccccgggcca cggtagctcc acattggaaa
     7861 actgccagtg cgcaacctgt accggttcat gcaatttaaa cagacaaatc ggtctgccat
     7921 tgatcatttt ttctgacaaa agctcgccac actgttcaaa accgcgacgc cagcgctcag
     7981 cggtgacgtt ttgatggcaa cgcaaagaaa tgtgatcggc agtcagcgga gtgatatcca
     8041 accccagacg gcgggaaagt tcatctaatg cgtgggtaaa tcgcggtaaa tccgatgcaa
     8101 tatcctgcag ctcgtcgata gattgccagt tcgccataat cactcttcgt ctttcagtaa
     8161 aagcgttaat ttaccctgtt gccctgtgcc aaccaaccgc tgatttcacg ccgcttctga
     8221 tgcaatagtg aaaacggcaa tacgccacgc gcacgttgct gacgaaaaca gccatttgca
     8281 gtatactccc gccctaattt ctttaactgg tgcgggcaat ttttgctcgc ttcatcaatg
     8341 taaggtattc cggtgaatat tcaggctctt ctctcagaaa aagtccgtca ggccatgatt
     8401 gcggcaggcg cgcctgcgga ttgcgaaccg cagattcgtc agtcagcaaa agttcagttc
     8461 ggcgactatc aggctaacgg catgatggca gttgctaaaa aactgggtat ggcaccgcga
     8521 caattagcag agcaggtgct gactcatctg gatcttaacg gtatcgccag caaagttgag
     8581 atcgccggtc caggctttat caacattttc cttgatccgg cattcctggc tgaacatgtt
     8641 cagcaggcgc tggcgtccga tcgtctcggt gttgctacgc cagaaaaaca gaccattgtg
     8701 gttgactact ctgcgccaaa cgtggcgaaa gagatgcacg tcggtcacct gcgctctacc
     8761 attattggtg acacagcagt gcgtactctg gagttcctcg gtcacaaagt gattcgcgca
     8821 aaccacgtcg gcgactgggg cactcagttc ggtatgctga ttgcatggct ggaaaagcag
     8881 cagcaggaaa acgccggtga aatggagctg gctgaccttg aaggtttcta ccgcgatgcg
     8941 aaaaagcatt acgacgaaga tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa
     9001 ctgcaaagcg gtgacgaata tttccgcgag atgtggcgca aactggtcga catcaccatg
     9061 acgcagaacc agatcactta cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg
     9121 ggcgaaagcc tctacaaccc aatgctgcca ggaattgtgg cggatctcaa agccaaaggt
     9181 ctggcagtag aaagcgaagg ggcgaccgtc gtattccttg atgtgtttaa aaacaaggaa
     9241 ggcgaaccga tgggcgtgat cattcagaag aaagatggcg gctatctcta caccaccact
     9301 gatatcgcct gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt gctgtattac
     9361 atcgactccc gtcagcatca acacctgatg caggcatggg cgatcgtccg taaagcaggc
     9421 tatgtaccgg aatccgtacc gctggaacac cacatgttcg gcatgatgct gggtaaagac
     9481 ggcaaaccgt tcaaaacccg cgcgggtggt accgtgaaac tggcagacct gctggatgaa
     9541 gccctggagc gtgcacgtcg tctggtggca gaaaagaacc cggatatgcc agctgacgag
     9601 ctggaaaaac tggctaacgc ggttggtatt ggtgcggtga aatatgcgga tctctccaaa
     9661 aaccgcacca cggactacat cttcgactgg gacaacatgc tggcgtttga gggtaatacc
     9721 gcgccataca tgcagtatgc ttacacgcgt gtattgtccg tgttccgtaa agcagaaatt
     9781 aacgaagagc aactggctgc agctccggta atcatccgtg aagatcgtga agcgcaactg
     9841 gcagctcgcc tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg
     9901 catgtaatgt gtgcttacct gtacgatctg gccggtctgt tctctggctt ctacgagcac
     9961 tgcccgatcc tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa
    10021 ctgacagcga agacgctgaa gctgggtctg gatacgctgg gtattgagac tgtagagcgt
    10081 atgtaatcga tttttcgtga gagtgaagcc tgatcagggt tagccgatca ggctttttta
    10141 ttgccatcta aatgtattct gaaaacggac atgccattgt tttctcactg ttgagtaaga
    10201 ggccagaagc gtaatatcca ggcccaggaa aacgataacg gttcaattta aggaataccg
    10261 aagtgtttaa atttcttgta ttaacattag gcattatctc ttgccaggct tacgcagaag
    10321 atacggttat agtaaacgac catgacactt cagccatcaa agattgttgg caaaaaaatt
    10381 cagatgatga tactgacatt aacgtgatca aatcatgcct gcgacaagaa tacaatctcg
    10441 tcgatgcgca attaaataaa gcctatggtg aagcttatcg ttatatagaa caagtgccac
    10501 gcacaggtgc aaaaaaactt gatactgaac aacttaactt gcttaaaaaa tcacagcgag
    10561 cctggctgga ttttagggac aaagaatgtg aattaatcct ttcaaatgag gatgttcagg
    10621 atttaagtga cccctattct gaatcagaat ggctatcatg tatgatcata cagaccaata
    10681 cgcgaactcg ccagttgcag ctataccgta actctgaaga tttttatcca agccctttga
    10741 caagaggata attcacatct ttttggcatg ttttgttgca agctattcct gataaatgat
    10801 tgcaacaaaa catccagcct tttgactgag ttattaaaca tactcgcgag cgcgtaattt
    10861 tttgtctttc agcgataatt cacaatcact tcattccgtt gtacctttag cggtggaatt
    10921 aaccgccctg aaccgccccg ggtttcctgg agagtgtttt atctgtgaac tcaggctgcc
    10981 agatcatcgt ttccgatgga agcataataa gctttttctg cttctgccgg aggagt
//
LOCUS       DAJTSA010000124        10946 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000124 DAJTSA010000000
VERSION     DAJTSA010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10946
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.124"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OO369_003509"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OO369_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR5983649.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="OO369_003510"
     CDS             complement(1433..1759)
                     /locus_tag="OO369_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCR5983650.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="OO369_003511"
     CDS             1894..2235
                     /locus_tag="OO369_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR5983651.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="OO369_003512"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="OO369_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="OO369_003513"
     CDS             complement(2833..3543)
                     /locus_tag="OO369_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR5983652.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="OO369_003514"
     CDS             3651..3956
                     /locus_tag="OO369_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR5983653.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..4475
                     /locus_tag="OO369_003515"
     CDS             4155..4475
                     /locus_tag="OO369_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase"
                     /protein_id="HCR5983654.1"
                     /translation="MSKNDRMVGISRRTLVKSTATGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQGTRLFARSLH
                     PPAY"
     gene            5035..5613
                     /locus_tag="OO369_003516"
                     /pseudo
     CDS             5035..5613
                     /locus_tag="OO369_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(6299..7288)
                     /locus_tag="OO369_003517"
     CDS             complement(6299..7288)
                     /locus_tag="OO369_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983655.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(7373..7864)
                     /locus_tag="OO369_003518"
     CDS             complement(7373..7864)
                     /locus_tag="OO369_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983656.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYGRRQMNKRAEAERIAKEQRLQAE
                     RMREENMTDAEWQKELQRREQVKAERRTYGESLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            complement(8185..8685)
                     /locus_tag="OO369_003519"
     CDS             complement(8185..8685)
                     /locus_tag="OO369_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983657.1"
                     /translation="MTSDNKQEYTIHIFGQDITSQDGLFRLDEIRRVGIQIGKVEDNR
                     STEVARFMRTKAGRSLTNPENGFVKKINMGHLGTSWLADPVAAIEFGRWLDLGYGLAV
                     TRAFVSMTSSATVQSAVASAEDIKPETKEFIQRHGTGLYIAGREVSQAEYSDYQAVTA
                     GRCEWR"
     gene            complement(8675..9190)
                     /locus_tag="OO369_003520"
     CDS             complement(8675..9190)
                     /locus_tag="OO369_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983658.1"
                     /translation="MNTELPVTNRQFLIILLTNAESSVDCCRYNLDLLKLSRWYAQAE
                     DWDYEKIREMYAEPIQSYLQTHTEEELKRGLQDIFYFNNPYTCEGDSEEMDWIASRSD
                     ADLKHISNAMMQVESKWSRRLQVLTEDYENGTFDKLVPPDAERLQAKADQEQRFKSFA
                     EALAGGASYDF"
     gene            complement(9621..9989)
                     /locus_tag="OO369_003521"
     CDS             complement(9621..9989)
                     /locus_tag="OO369_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983659.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
     gene            complement(10401..>10946)
                     /locus_tag="OO369_003522"
     CDS             complement(10401..>10946)
                     /locus_tag="OO369_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5983660.1"
                     /translation="YQFTRHTAHTTSEARRWASLVSVVTGARSAEVCHLTKRDIVTLD
                     NGLVCIDINEDGDGKSVKNKHSVRLVPLTDGAYGFDLTSFLSWVDMQPDEGPLFGMTP
                     SAYSSWFNSRVLTEALGDSQDVSLHSLRHWLATRMKERGVNLVDAQGILGHSSQSITY
                     DLYGKGHAVGRLADVLKTALL"
BASE COUNT         2810 a         2783 c         2705 g         2648 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatgtataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgacaggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag ggtacgcgcc
     4441 tgtttgcgcg gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaatgaccca
     4501 atgaaacgcg tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc
     4561 ctggatacta tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaagctgta
     4621 tatattcagt actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc
     4681 tcggcggtca aacgcctgat gaactgctac ggcggttcac tcaatcagta tggctcctac
     4741 agcaccgcgc aaatttcctg tgccatgccc tacacctacg gcagtaatga tggcaacagc
     4801 accacggata ttgaaaacag caagctggtc gtgatgtttg gtaacaaccc ggcagaaacc
     4861 cgcatgagcg gtggtggcat cacttatctt cttgaaaaag cgcgcgagaa atcgaacgcc
     4921 aaaatgattg ttatcgatcc gcgatatacc gatacggctg ccggtcgtga agaggaatgg
     4981 ctccctattc gcccgggcac cgatgccgcg ctggttgcgg gtattgcctg ggtattgatt
     5041 aacgaaaatc tcgttgatca accttttctc gataaatact gcgtcggcta tgacgaaaaa
     5101 accttacccg cagatgcacc caaaaatggt cactataaag cctatattct tggtgaaggt
     5161 gacgataaaa cagcaaaaac gccgcagtgg gcttcgcaaa ttaccggtat cccggtagac
     5221 cgtatcatca aactggcgcg tgaaattggc acaacaaaac ccgcttatat ctgctagggc
     5281 tgggggccgc aacgccaggc aaacggcgag ctgactgcac gcgctattgc tatgttacct
     5341 attttgacgg gcaatgtcgg catcagcggc ggaaatagtg gcgcgcgtga atcgacctat
     5401 accattacga tagaacgcct accggtgctg gataatccgg tcaaaacgtc aatctcctgc
     5461 ttcagctgga cggatgctat cgatcatggc ccgcaaatga cggcactccg cgacggtgtc
     5521 cggggcaaag ataaactgga tgtgcccatt aagttcatct ggaactacaa ggacttgaag
     5581 cggaccctta gtaggcctga ggtcctgcat tgagtcaaga ccttgtgtca ttctttgtgc
     5641 cagacttgtg tcattagacg ggatgtctag acggctggtc aggaccctcc ggtggcctcg
     5701 gtctggactt gaggcgggct atgggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagta caaacctaag cccccagaag
     5821 cccacccaca gccttagaca gagtgtcgaa tacattcaca ccgagtcgac cggagaggac
     5881 catatagagg cccacagaga ggaacacgag tttatagacc agagtcagga gggaccttag
     5941 gtcttcggaa gtcatggaca taaagccccc ccctatcttc tattgcacta ccgcactaga
     6001 caactagtac aaaacaaagg cccagaccat tggtccagac cgtagttagt taatcacagt
     6061 gacaagccac cacctgaagt cctgctgata ttcagtccag acttacctct gctcatggtc
     6121 ttacctccct cggtctcctg accttgggta tcgactacag cttgctgagc gtcatctgga
     6181 ggagctacag gaggagctac aggaggcggg accgaagccc caccttgagt cccaccacca
     6241 cctgtcaggc ccttggcgat ggacctgaag cctttactaa tgctcttacc cattggtctt
     6301 acgcttcggt cttggtgaac ttcaggcgtt tgactacagg caggacggca gtagctgagc
     6361 cagtgcccat cttgatggtc gtagcagctt cagcgacaac gaacacttta ccgaggattt
     6421 ccaagtcgtg ggactgagag tcgccatcac gtgaaaggac gaccgtaata ttctcaccgt
     6481 cgtgacggaa gcagccgaag ctcagggccg tgaagcgttt agccagcatg aatacaccct
     6541 tgtcttcacc gttgtaatag gcgatgtctg tccccagcat atcgacgata gcgtcttcac
     6601 cggagaagcc ttgcttagca gccagacggg acagtgcttt cagttggctc tcatgagcga
     6661 taataccgta gtcagacacc tcagaaccga aagaagactc tgtgttctca tacagagcgt
     6721 ccatgatagc gtctgcctta gcggtcaggc taccagtcag gacaggaagc tctacggtct
     6781 tcagcagctt agaggattcc agaacgtcga ccatgcgagc gttgatggta cggaccacat
     6841 tctcacagat gttcatagcg tcctgctgaa gtgtgccctg agtgaatccg gtggatgact
     6901 tggcgtccca gagcttggtc tgctcagaga tgagaatagc ctgctcgatt ggttcgacca
     6961 cgatttcttg tccagacgga acggtcacga cttcaggtcg cttgccacca cggacaggaa
     7021 cgaccacaga gcggtcagcc agagtgtgag caatgattgg ggtcggtaag ctgttacggg
     7081 tcggcaggac ttctacaggg aacgggatgt tctcttgagc gtctttaagt acaccgttca
     7141 gcatagcctt aaccgtctta gctaactgga gggcatagac ctgcgggtca gcgttagtcg
     7201 gcagaccttc attgacctga atgttgtcgg tatcggtgat gcgtactggc tcaatgccaa
     7261 actggaaagc gtctttaatc gtagtcataa taatcaatct cctaaattag tttaaatact
     7321 gtgagtcctc aaaactgagg agtccctaac atcgggtcag ggactgtgtg ttttacaagc
     7381 tgtccatcca gttaccgcct gactcaaggt cagcgacaat agcggcacgg gagcgaccag
     7441 cggaatgggt agcactgcgg aggctctcac cataggtccg gcgttcagct ttgacctgtt
     7501 cgcggcgctg tagctctttc tgccactctg catcggtcat gttctcttca cgcatacgct
     7561 cagcctgaag tcgttgctct ttagcgatac gctcagcttc agctcgtttg ttcatctggc
     7621 ggcgaccata ggcggtcatc aagtcattac tgaagaagtt gccaatggtc acgccagctt
     7681 cagccagagc ttccagtaac tccattgcct cgcggctggt agacggcagg cctgacaggt
     7741 agcccggaat gagggtcttg atagcgagcg acagcggata gggctgaccg tccagactca
     7801 cagtgttttc ataaaggttg atgtcggtga ctgacagctt ccagttgttg ttgttgaaca
     7861 tcataggtaa ttctcctctt gagagttgag actatcggta tcgcagacgc agtagcgcct
     7921 acgggtctcc ccgcatcggt catctcaaaa ggtttaaaat taggtttggt atggtcagga
     7981 ctgcttagca aaggctcggc gtaactgatt gtctaaatca cggtccacca gcatagctat
     8041 cagctcgtct cgactgtagt aagggcgctc agggtcttcg actccaagtc tcagcagaga
     8101 cagcagatga cgtgtgggca ggccacggta acgaagggtc gctttgccga taggtgtacg
     8161 agggtcaatc atagccatga ttacttacct ccactcacag cgaccagcgg tcacagcttg
     8221 atagtctgaa tactcagcct gtgagacttc acgtccggcg atgtagaggc ctgtgccatg
     8281 acgctggatg aattccttgg tctccggctt gatgtcctca gcagacgcta cagcagactg
     8341 cacggtggcg ctggaggtca tagacacgaa ggctcgggtt actgccagtc cgtacccaag
     8401 gtccaaccac cgaccgaact ctatagcggc tactgggtca gccagccacg aggtccctaa
     8461 gtgacccatg ttaatctttt taacaaaccc gttttctggg ttggttagac ttctaccagc
     8521 cttggtcctc atgaagcgag caacctcggt actacggtta tcttctacct taccaatctg
     8581 aatgcctacc cggcgtatct catccagtcg aaacaatccg tcctgactgg tgatgtcctg
     8641 accgaagata tgaatggtgt attcctgctt attgtcagaa gtcataagag gcacctcccg
     8701 ccagagcttc ggcaaaggac ttgaagcgct gttcttggtc agctttcgcc tgaaggcgtt
     8761 cggcatcagg tgggaccagc ttatcaaagg tgccgttttc gtaatcttca gttaggacct
     8821 gaaggcggcg ggaccatttg ctctccacct gcatcatcgc gttagagatg tgcttcaagt
     8881 cggcatcaga gcgtgacgct atccaatcca tctcttctga gtcaccctca caggtgtaag
     8941 gattgttgaa atagaagatg tcctgcaggc cacgcttcag ctcttcctcg gtgtgtgtct
     9001 ggaggtatga ctggataggc tccgcgtaca tctcccggat tttctcatag tcccagtctt
     9061 ccgcctgggc ataccatcgg gataacttca gcaggtctaa gttatagcga cagcaatcga
     9121 cactggactc tgcattggtc agcaggatga ttaagaactg gcggttagtt actggtaatt
     9181 cggtattcat acaaagatgc tcctttggta aagcactttc tgtctccaca tggagtccgc
     9241 accattcaga cgcatcctaa acggacccat cagaaagtgc ttaacgaaag tgaatggtta
     9301 aatgttgacg gtctggagtg accatgttct ccatgtacgt ataaaggtgc gaccgcatgt
     9361 caggacgagc tgtaaggact ccagatggtg agcggtgata gacttaagcc aatgtagtga
     9421 gtagacgagc tactaaccga gacgactaaa gaacgctaac gagctggagg tcgatacagg
     9481 aggtctgtca gaggcggtca cgggccttga ttacttagga gagatactta atgtcggttg
     9541 accagatgct cgcatgttct gacgcatcag actcagaggc ggaggctggt ctccctatag
     9601 tgatagttta gggtccgtaa tcatgaacca gctttactgg cgtggtactg gacggattta
     9661 ctggcgctga cgacggtagc atagacgtag cgagcgtagc gagttgaaac cataggttca
     9721 cccaaggcaa cacgcttacg gctcagggac tcctgcacag cgcttacagt taatgtgtcg
     9781 tgcgttagta gttgggtcag aatcaagtat gggctgacag gcttggtatt gcttgccatt
     9841 gattcgttca ggcgaccagc accagtaagg acatcatcca gccaaccact caggtcgccc
     9901 tgaccatcga atgacagcag gtccccgcag tgctgctgca agtaggcttt ggtgaacgtg
     9961 ttgtcgtgag ttgcgattgg tcgtgccata gagtatctcc tgtagttgaa ttagttggtt
    10021 cccctatagt gataatcaag tccatcactg gtgatgcata cagtagcgcg acaggatgtg
    10081 actcacaggt tgaaccgaat gtcaaggact aaagacgcct caggaattaa ggaagtatat
    10141 ataaagagag accagaggcg actcacagtt aaacaggaat gtcaagagga gaccatagga
    10201 atttggaggg actggagttt aggacgcagt ttaaataaga ccacagacag acttagagtc
    10261 ggacagccga aggcggacca gtgggcctat agtcaggagg gattatgatt attatcaccc
    10321 tccctaactt cagtcagact tcagtgaccc aaggtctcaa ccatcaagcc gtatatggtc
    10381 tccaccttgg gtctccctac ttaaagtaaa gctgtcttaa gcacatcagc cagcctaccc
    10441 acagcgtgtc ctttcccgta caggtcatac gtaattgact gactgctgtg acctaagatg
    10501 ccttgagcgt ccaccagatt gactccacgc tctttcattc gggtcgccag ccaatgtctc
    10561 agactgtgga gagagacatc ctgagagtct cctagggcct cagtcaggac ccttgagtta
    10621 aaccagctag agtaggcgct cggagtcatc ccgaagagag gaccctcatc aggctgcatg
    10681 tctacccacg acaggaagga cgtcaggtca aacccataag cgccatcggt taacggcaca
    10741 agtcggactg aatgtttgtt cttcacagac ttcccgtccc catcttcatt gatgtcgata
    10801 cagaccagtc cattgtccaa ggttacaatg tcacgtttag tcaggtggca gacctcagca
    10861 gaccgagcgc cagtgaccac agacaccaga gacgcccatc tacgggcctc agaggtcgtg
    10921 tgagccgtgt gtcgtgtgaa ctgata
//
LOCUS       DAJTSA010000125        10927 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000125 DAJTSA010000000
VERSION     DAJTSA010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10927
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.125"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..203
                     /locus_tag="OO369_003523"
     CDS             <1..203
                     /locus_tag="OO369_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983661.1"
                     /translation="GASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAI
                     YLLAGCLISAMTALLMKDNQRS"
     gene            305..1759
                     /gene="amn"
                     /locus_tag="OO369_003524"
     CDS             305..1759
                     /gene="amn"
                     /locus_tag="OO369_003524"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR5983662.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2102..2818
                     /locus_tag="OO369_003525"
     CDS             2102..2818
                     /locus_tag="OO369_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5983663.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3274..3349)
                     /locus_tag="OO369_003526"
     tRNA            complement(3274..3349)
                     /locus_tag="OO369_003526"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3314..3316),aa:Asn,seq:gtt)
     gene            complement(3450..5077)
                     /gene="yeeO"
                     /locus_tag="OO369_003527"
                     /pseudo
     CDS             complement(3450..5077)
                     /gene="yeeO"
                     /locus_tag="OO369_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            5082..5157
                     /locus_tag="OO369_003528"
     tRNA            5082..5157
                     /locus_tag="OO369_003528"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5115..5117,aa:Asn,seq:gtt)
     gene            complement(5195..6145)
                     /gene="cbl"
                     /locus_tag="OO369_003529"
     CDS             complement(5195..6145)
                     /gene="cbl"
                     /locus_tag="OO369_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR5983664.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6247..7164)
                     /gene="nac"
                     /locus_tag="OO369_003530"
     CDS             complement(6247..7164)
                     /gene="nac"
                     /locus_tag="OO369_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR5983665.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7492..7567
                     /locus_tag="OO369_003531"
     tRNA            7492..7567
                     /locus_tag="OO369_003531"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7525..7527,aa:Asn,seq:gtt)
     gene            complement(7623..8558)
                     /gene="ldtA"
                     /locus_tag="OO369_003532"
     CDS             complement(7623..8558)
                     /gene="ldtA"
                     /locus_tag="OO369_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5983666.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8620..9699)
                     /gene="cobT"
                     /locus_tag="OO369_003533"
     CDS             complement(8620..9699)
                     /gene="cobT"
                     /locus_tag="OO369_003533"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR5983667.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9711..10454)
                     /gene="cobS"
                     /locus_tag="OO369_003534"
     CDS             complement(9711..10454)
                     /gene="cobS"
                     /locus_tag="OO369_003534"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR5983668.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10451..>10927)
                     /gene="cobU"
                     /locus_tag="OO369_003535"
     CDS             complement(10451..>10927)
                     /gene="cobU"
                     /locus_tag="OO369_003535"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR5983669.1"
                     /translation="SSQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDEL
                     IHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQR
                     CPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         3037 a         2778 c         2366 g         2746 t
ORIGIN      
        1 ttggtgccag ttatcgctac agtggtgctg gagtcggtta tcaggttgcc agtgtggttg
       61 gcggtggatt tacacctttt attgccgctg cactcatcac ttactttgcc gggaactggc
      121 atagcatcgc catttatttg ctggctggat gcctgatttc tgcaatgacc gctttattga
      181 tgaaagacaa tcaacgctcg tgatagcctg gcgaagatca tccgatcttc gccttatact
      241 tttgtttcac attcctgtga catactatcg gatgtgcggt aattgtatgg aacaggagac
      301 acacatgaat aataagggct ccggtctgac cccagctcag gcactggata aactcgacgc
      361 gctgtatgag caatctgtag ttgctttacg caacgccatt ggcaaatata ttacaattgg
      421 cgaattacct gatgaaaacg cccgtaaaca aggtcttttt gtctatccat cactgaccgt
      481 aacctgggac ggtagcacaa caaatccccc caaaacgcgc gcatttggtc gttttactca
      541 cgcaggcagc tacaccacca cgattactcg ccctactctc tttcgttcgt atctgaatga
      601 gcaactaacg ttgctttatc aggattatgg tgcgcatatc tctgtgcaac cctcgcaaca
      661 tgaaatccct tatccttatg tcatcgatgg ctctgaattg acacttgatc gctcaatgag
      721 cgctgattta actcgctact tcccgacaac agaactggcg caaatcggcg atgaaactgc
      781 agacggcatt tatcatccaa cggaattctc cccgctatcg cattttgatg cgcgccgcgt
      841 cgatttttcc ctcgcacggt tgcgccatta taccggtacg ccagtagaac attttcaacc
      901 gttcgtcttg tttaccaact acacacgtta tgtggatgaa ttcgttcgtt ggggatgcag
      961 ccagatcctc gatcctgata gtccctacat tgccctttct tgtgctggcg ggaactggat
     1021 cactgccgaa accgaagcgc cagaagaagc catttccgac cttgcatgga aaaaacacca
     1081 gatgccagca tggcatttaa ttaccgctga tggtcagggt attactctgg tgaatattgg
     1141 cgtgggaccg tccaatgcta aaaccatctg cgatcatctg gcagtgctac gcccggatat
     1201 ctggttgatg attggtcact gtggcggatt acgtgaaagc caggccattg gagattatgt
     1261 ccttgcacac gcttatttac gcgatgacca cgttcttgat gcggttctgc cgcccgatat
     1321 acctattccg agcattgctg aagtgcaacg tgcgctttat gacgctacca agttagtcag
     1381 cggcaggcct ggtgaggaag tcaaacagcg gctgcgtaca ggtactgtgg taaccacaga
     1441 tgacaggaac tgggagttac gttactcagc ttctgcactt cgttttaact taagccgggc
     1501 cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt tccgcgtgcc
     1561 atacgggaca ctactgtgtg tttcagataa accgttacat ggtgagatta agcttcccgg
     1621 ccaggctaac cgtttttatg aaggcgctat ttccgaacac ctgcaaattg gcattcgggc
     1681 gatcgatttg ctgcgcgcag aaggcgaccg actgcattca cgtaaattac gaacctttaa
     1741 tgagccgccg ttccgataat aagttatgaa ccttcttatc agaaatagat gtgaaggatt
     1801 aagtaagaca tctggcaaat agcctgcaat aaacgcgggc tgcttgcttc cttgggcaga
     1861 tgcgagtttt attatcgtct taatgatttc cacatattaa aagcaagtat gctttcaaaa
     1921 cacaattata aaaaatcccg ccaacaacat tagtttttat aaaattaaat ataagattat
     1981 ggctttagaa tatttttatt tctaatagac gagatttttc ctgttatgat ataatatgcc
     2041 gaattaacac atgttaacga tttaccagta atgtaaataa attttcgagg agatcattcc
     2101 agtgggacgt aaatgggcca atattgttgc taaaaaaacg gctaaagacg gtgcaacgtc
     2161 taaaatttat gcaaaattcg gtgtagaaat ctatgctgct gccaaacaag gtgaacccga
     2221 tccagaatta aacacatctt taaaattcgt tattgaacgt gcaaagcagg cacaagttcc
     2281 aaagcacgtt attgataaag caattgataa agccaaaggc ggcggagatg aaacgttcgt
     2341 acagggacgt tatgaaggct ttggtcctaa tggctcaatg attattgctg agacattgac
     2401 ttcaaatgtt aaccgtacga ttgctaacgt tcgcacaatt ttcaataaaa aaggcggcaa
     2461 tatcggagcg gcaggttctg tcagctatat gtttgacaat acgggtgtga ttgtatttaa
     2521 aggaacagac cctgaccata tttttgaaat tttgcttgaa gctgaagttg atgttcgtga
     2581 tgtaactgaa gaagaaggta atattgttat ttatactgaa cctgctgacc ttcataaagg
     2641 aatcgcggcg ctaaaagcag ctggaatcac tgagttctca acaacagaat tagaaatgat
     2701 tgctcaatct gaagttgagc tttccccaga agatttagaa atctttgaag ggcttgttga
     2761 tgcccttgaa gatgacgacg atgtacaaaa agtttatcat aacgtcgcaa atctctaatt
     2821 atattttaaa gaaatctgtc tttatggcag atttctttaa tctcatataa ttcttataaa
     2881 aaatataata tttaactcgt catattgctt ataaaaaatc cccgtcccca gagaaataat
     2941 atttattaaa atggcaggtc ttctttttct gattaccgaa gacaaagtgg caattacgta
     3001 tagtttcccg ataaagacgc gacagctacg tcccgcataa ggcatttttc tctttatctt
     3061 tgtacggtac ttcatggaac agaatttttg accttgtgaa tcgtgatatc tgttggggag
     3121 gggcaatttg ctcactgaag cgtgagactc gattaagcgc acgaaacaca gaaatcaaaa
     3181 aacccggcca cttttttaca aagtggccgg gtaaaaataa tttttatttt ttaactgtcc
     3241 tgagtctcat agagatgtct caaaactaaa atttggctcc tctgactgga ctcgaaccag
     3301 tgacatacgg attaacagtc cgccgttcta ccgactgaac tacagaggaa tcgtgagaac
     3361 gaggcgaata ttagcgatgc ccacccacaa tgtcaaagcc tgttttttaa atttgaaatc
     3421 gtttgctgaa ataatctgca ttttgtcgtt tattccgaca caactggctt tttttcacac
     3481 ttttgcggct cggatcgagg gtatttccac agccaacgtc cagtaaccat tcgccagtaa
     3541 aacagcacgg cccgcacagc ccagtcggca aacattccca tccagacacc aaccacaccc
     3601 cagccaagca tgattccctg cacataaccg actacaaccc ggcaacccca catgctcaac
     3661 atagaaaccc acatggcgta acgggcatca cgagcaccct taaatccagc gggtagcacc
     3721 catgaggcgg accaaatagg cataaataaa gcatttagct aaatcagaat cacaacgacg
     3781 tgtttaacct gtggatcctg ggtgtaaaac gatgccataa ccccggcaaa gggagccgtt
     3841 agccaggcga tggccgttaa tccaagagtg gacagccaga acacatgccg caactgaatc
     3901 tctgcttgcg ctatctgccc tacccccaac cttcggcctg taatgatcgt agaagcagaa
     3961 ccgagcgcac ttccgggtaa gttgataaga gccgcaattg agaacgcgat aaaatttccg
     4021 gcaataacgc tggtccccat cccggcaacg aacatttggg ttaataaccg accactggta
     4081 aataacactg attcgacact cgctggaata ccaatcccca tgacttccca gataatgcta
     4141 aaattcagcg gtttaaaata gctctttaac gaaatcctta gcgcaggatt aaaaccaatc
     4201 gccagcaccc acaaaattgc aactgcgcca atataacgag aaatggttaa acccaaccca
     4261 gccccgacca ggagaaaagg ccgtaaatca atatgccgct aataataata ttaagaatat
     4321 tcaggctacc gttaatcagt agcggtattt tcgtattccc tgcaccacga agtgccccgc
     4381 taccaataag agtgatggca gcggctggat aactgagtac ggtcagctcc agataagtca
     4441 acgccagtgc tttaacttct gtcgtggcat cacccgcgac gaaatcaata atttgttcgc
     4501 caaaatgatg aattagcgtt gccaacagta cggcaaacaa cgtcatgatc accaatgact
     4561 gccgcgtcgc caccctcgct cgtcgtcgat cccgcttacc gagactaaat gctacaacga
     4621 cagtagtacc aagatcgata gctgcaaaaa aagccataat gaccatattg aagctgtccg
     4681 ccaaccccac gccggccatc gcatcttttc ccagccaact gaccagaaaa gtgctcagga
     4741 cccccatcaa caggacacag gcgttctcca tgaagatagg aacagcaagc ggggttatct
     4801 cgcgctagaa caacactttg tagctcttgc gtttagagtg ccagcgagtg ccgtgaacaa
     4861 cctggcgtaa agcagaggag atattcaaag ccgaccttaa ttgcagaaag tgaaaccaca
     4921 tttcaaatga tgagggagaa tcagcaaagc tgcaaagatt ttcgccaaca aattgtctgc
     4981 aaatgcaaca aactgttgat agaaacggca aacagttggg aaatttaaaa atcgggtttg
     5041 acaaaatatt tttcgccgtt aagatgtgcc tcaacagcga ttcctctgta gttcagtcgg
     5101 tagaacggcg gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaaat
     5161 ttctgaaaag cccgctttta tagcgggctt tttgctatat ctgataatca atttcctctt
     5221 cactgttttc catcacctgg cgcttgatat cctcaactga cagtcctgca ttacaaagtt
     5281 ccagaaagcg ccaaacatag ttacgctgaa gttgtcctcg cttcagtccc aaccagacag
     5341 tattagcatc gaaaagatgc cgcgtatcca ggcggattaa attctcttcc tcttgttcgc
     5401 cgctggattg ttcggcaact aatccgatcc caagcccaag agcaacatag gttttaatga
     5461 catcagaatc ctgtgcactt aatacaatat ctgccggcaa acctttgcgg gtaaatgcgt
     5521 catcagtgcg tgagcgcccc gtaattccct gtcggtaagt aattaacggc cacttcgcta
     5581 ttgattccag cgtcaatggt gtaatttgcg ttaagggatg atcgagtgga acaagcaaac
     5641 tatggtgcca acaaaaccat gggaaggcga cgagctgcgg gtcattactc aaacgctcgc
     5701 tggcgatacc aatatcagct tcgccatttt gcaacaacgt cgcaatttcc tgtggcgaac
     5761 cctggattag ctcgagccga acctccggga aaagttcgcg aaaagcttta atgacctctg
     5821 gcaagctata acgtgcctga gtatgcgtcg ttgcaatagt gagaacacca gacgtatcgt
     5881 tggtaaacag gtctgcaagc cgacgaacat tactggcttc attcagaata cgttctgcaa
     5941 tgaccagtaa tgctttgccc ggttcagtca tccccagcag tcgcttacct cgtcgaacaa
     6001 atatttcgat gccaaattca tcctccagtt cccgaatatg acggctaacg cctgactgtg
     6061 aggtaaaaag catattcgca acctctgtca ggttgtaatc ctgacgtgca gcctcgcgga
     6121 ttatctttag ttgttggaaa ttcacggtaa actccgggca gttcagatat cccgttattg
     6181 ttaaagtcta atgcccggca taacaaataa taaaaacccg catcttattc catcccgata
     6241 taacgcttag ctcaccaatt gccactgcct tttttccatc actggagaac taatcactga
     6301 cattaacaac tctttcactg cctgtgcctg tggtgacaag tttgctctgg cgggtaaatt
     6361 taaggacaaa gagagactca ttgaaggagt ggtaatgcgt gacatccacc catttactgc
     6421 gccacataac gaacgcgcag ccgattcagg taatactgca acgcccatgc cgctggcaat
     6481 cgctgcggta agcgtggcaa tagactcaat ttcaccaatg acttttgccg tgagccgccg
     6541 tagggaaaaa gcctcatcaa cacgaagtct aacagcgctg taatcactgg ggagaaaaag
     6601 gttcatttgc gcaatagcat taacatcaac gttttgcccc gggcaatctt gagttcctac
     6661 caggaaaaga tcttctttca gcaaagcctg actggatacc ccagccacag gggaatgctc
     6721 ataaatcacc gccatatcga gttggtgatt tatcagtttt tcgttaagca ctgcaccact
     6781 attttcatga agatagataa cgacctccgg gaattcagcg cgaaccgcct gtaataaggg
     6841 catggtgatg gatgacgcag cggttcctgg cgcaaagcca atcgagactt gccccgataa
     6901 tgactgacca acgttatgca ccgccagttg ggcctgctca cactgacgta aaatggcccg
     6961 tgcatgggta tagagaattt ttccggcgtc tgttggcgta acgccccgtt ttgtgcgaat
     7021 caaaagctgt tgatttaact caccttccag tatggcaacc tgttggctga gcgctggttg
     7081 tgcgatatgc aatacttcag cagcctgggt caggctacca atatctacaa tttttacgaa
     7141 gtatttcagg cgtctgaagt tcatgttgcc tccggttttt aagaatcggc ccaagtgccg
     7201 ccattactta caaccagtat gcaagatgcc tgccagttct attttggcgt tgatgcgcaa
     7261 gctaaccaac tgtcaaataa gagattatga tagattcgtc attggccctt tttatcagca
     7321 gtcgcattca cctgccctat aaaaggaggg tatgcaccac gatggttcat tacccaataa
     7381 gattgaaagc tcaccacttt gttgaaattg gcagcaaaca aacaaaaaaa atgcatttca
     7441 ccctttgaca tcaccatgca ctgccattaa tatgcgcccc gttcacacga ttcctctgta
     7501 gttcagtcgg tagaacggcg gactgttaat ccgtatgtca ctggttcgag tccagtcaga
     7561 ggagccaaat tcaaaaaagc ctgctttcga gcaggctttt tactttctaa ttaccaacgc
     7621 tcttaaaaca tctgtcttga accagaacta atttgcacag gcattcccga tcgatgttgc
     7681 aacgcagcat ttgcgcgatt tacatcaact tcttgcccgt tgataaacgc ccgcaaagat
     7741 ggggttaccg gcaatggcac ttttcggtca gactcatatt ctgcacgatt gcgcgacagc
     7801 ggctcatgaa cttccagcca ctttgaacca tccggttccg ttgtgtattt cactggctga
     7861 tcaataattt gtacacgagt gcctacagga acattatcaa acagatattt gatatcgtca
     7921 ttacgcagac gaatacagcc ctgacttaca cggagcccaa taccaaaatt ggcattggta
     7981 ccgtggatgg catacaacct gccaatataa atcgcgtaca gccccatggg gttatcgggc
     8041 cccgcaggaa caaatgcggg caaactctcc cctcgtttcg catattcgcg ccggatgttc
     8101 ggcgttggcg tccaggttgg tgcttcttgt ttacgttcaa cggtagtcac ccagttacgc
     8161 ggggtttctc gcccagcctg gccgatacca ataggaaaga cttccactgt attactgtct
     8221 ggcggatagt aataaagacg catttcagcg acgttaacca caatcccttt acgaacagtg
     8281 gcgggcaaaa tcagttgctg cggaatggtg agttgtgagc caggcttcgg caaaaaaaca
     8341 tcagcgcccg gattcgcttc cagcatatta cttaaccctt gcccgtattg tgcggcaaaa
     8401 gtctccagcg gctgagtatt gtgatcagga acagttacag taagcgactg ccccactaaa
     8461 cggctaccct ctggaggtaa tggataagtt accgccaggc tattttggct ggcaaaaagc
     8521 agagcaaatg agcaaagaat atttacacga cgcatcatgt ccctttccta agtcgcgaat
     8581 actatccgat aagtatagct tttatcagac ttttcgtttt tagctgttca aatcagaagt
     8641 cgtattcccc ggtagaacaa tattactggc agcaagttcg cccatgttgt tgtatatcgc
     8701 acaagcagct tcgatgatgg acatcgccag agctgcacca ctcccctcac ctaaacgcat
     8761 atccatattg agataaggct ccagccccaa atgcgagagc gctatacgcg cgcctttttc
     8821 tgccgacaag tgagaaggaa tgagatacgg tttgattgca ggagacatct ggcaggctgc
     8881 gagcgcagca gcataagaaa gaaatccatc cagcaacacg ggtaaaccgc aggaagcagc
     8941 acctaacatc actccagcta ttccgaccaa atcaaatcca cccacttttg ccaggacatc
     9001 aacaccatcc tgaggatttg gttgattcaa cgtaatcgcc cgacgcacaa catcaatttt
     9061 attagccagt ttatctgtcg gcaggtttgc gccaatccca accacttctt caggatcccg
     9121 gccagtgatt gtgctgacta ttgccgctgc cggtgtcgtg tttgccatcc ccagttcacc
     9181 tacaccaaac agcgtgacac cgtttttcgc cagctcccgc gtataacata tgacgtccaa
     9241 aagcaacttt tcagcctgac ggcgactcat tgccggagct gaagcaatat tgccgctacc
     9301 tcgtgcgaca cgcatgttga taagcccggg gataggctca gcagtatcaa taccaacatc
     9361 aattacgtgg acgttagcgc ccgcttgtgc tgccagcaca cacacgccgg ttgttccacg
     9421 ggtcatattt tcagcctgta tggctgtcac ttcttttgga gaaatagcga ccccttcctc
     9481 ccagacgccg tgatcggcac acataaccag taccgctttt ttgcccacat gcggtatgcc
     9541 attcaccccc ggcattcctg ccagttgtat ggcaagcgcc tccagctttc ctaggctacc
     9601 aacaggtttg aataacccgt caacatgccg ttgtgcacgc gacatagcgg cagaattgat
     9661 ggctgggatc gtattcagta aatcggcaat tatttgcatg tcacgtttct ttatagcagt
     9721 gccagcagga agaccagttc accaagctct atggctgctc ccagcgtatc gcccgtttgc
     9781 ccgcccagcg tacgtttgag aagctgaccg aggatgaaaa tcgccaccat cgtcaccacc
     9841 atagcagcca caccatgcat acccagcaat aatacggcag caaaaatagc ggctaagccg
     9901 agtgtgacgc aggtttgtcg cccatcaatt ttgccaatga acacattgcc aagcccttct
     9961 tcccgcgcat aacgatgacg atacatcaat aatgcggcag tcccgcggct gaccgcacat
    10021 gctgccgcca gcgatgcaag gatcggttcg ccacgcagag ccaactcgct taacaccaga
    10081 atctttgcca gtaccacaaa aatcaatgcc agaccgccgt gggtgcctaa acggctatca
    10141 cgcatgattt ccaacatgcg atcgcgacta cgtgcagaaa atacgccatc acaggtatca
    10201 gcgaggccgt ccagatggaa ttccccggtc atcagcgcca gcacaagtac gctaaacagt
    10261 gccgccagtg gtgcgccaca ccatgcctgc agcaccatga agaccagccc gctaatcgcg
    10321 ccaagcaata atccaatcaa aggaaaagta ataataccgc gagaataatg ttcgaaatcc
    10381 agtccctggg accagcgacg cggtacaggc agacgcgtaa taaatgagag catcgcccaa
    10441 aataatttgc tcatttgatt tttactccaa tacccgaaac caccagccat acttcatttg
    10501 ccgcagcggc caactgctga tttacccgcc cggcaatatc acgaaaatgt cgtgccagac
    10561 gactctccgg cacaatcccc attcccactt cgttagtcac taatacaacc tttgcggggc
    10621 aacgttggca ggcagcaatc aacgactgaa tctcagcatt aatcgcctgt tccatcgcct
    10681 gataatccca ttcatcaggg tctttatcgc cgccataatc aaacaacaga ttagtcacca
    10741 ttgtggtaac gcattccagc aacacagcct cattcgggtt aatgtctgca tgaattaatt
    10801 catcaagatg ttgccagcgc tccactgtgc gccagtgctc cgggcggctt tgccgatgat
    10861 gttctatccg tgcagccatc tcatcatcaa ggatttgcga ggtagcgata tacagaacct
    10921 gtgaaga
//
LOCUS       DAJTSA010000126        10464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000126 DAJTSA010000000
VERSION     DAJTSA010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.126"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            3..203
                     /gene="cspG"
                     /locus_tag="OO369_003536"
     CDS             3..203
                     /gene="cspG"
                     /locus_tag="OO369_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001990088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCR5983670.1"
                     /translation="MTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNEN
                     QKVEFSIEQGQRGPAAANVVTL"
     gene            214..402
                     /locus_tag="OO369_003537"
     CDS             214..402
                     /locus_tag="OO369_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR5983671.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            377..607
                     /gene="ymcE"
                     /locus_tag="OO369_003538"
     CDS             377..607
                     /gene="ymcE"
                     /locus_tag="OO369_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HCR5983672.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            597..770
                     /locus_tag="OO369_003539"
     CDS             597..770
                     /locus_tag="OO369_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR5983673.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(819..1892)
                     /locus_tag="OO369_003540"
     CDS             complement(819..1892)
                     /locus_tag="OO369_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5983674.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1994..4705)
                     /gene="torS"
                     /locus_tag="OO369_003541"
                     /pseudo
     CDS             complement(1994..4705)
                     /gene="torS"
                     /locus_tag="OO369_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4788..5816
                     /gene="torT"
                     /locus_tag="OO369_003542"
     CDS             4788..5816
                     /gene="torT"
                     /locus_tag="OO369_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR5983675.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5789..6481)
                     /gene="torR"
                     /locus_tag="OO369_003543"
     CDS             complement(5789..6481)
                     /gene="torR"
                     /locus_tag="OO369_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR5983676.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6568..7740
                     /gene="torC"
                     /locus_tag="OO369_003544"
     CDS             6568..7740
                     /gene="torC"
                     /locus_tag="OO369_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR5983677.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7740..10280
                     /gene="torA"
                     /locus_tag="OO369_003545"
                     /pseudo
     CDS             7740..10280
                     /gene="torA"
                     /locus_tag="OO369_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10277..>10464
                     /gene="torD"
                     /locus_tag="OO369_003546"
     CDS             10277..>10464
                     /gene="torD"
                     /locus_tag="OO369_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5983678.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2524 a         2684 c         2788 g         2468 t
ORIGIN      
        1 aaatgactgg tttagtaaaa tggtttaacg cagataaagg ttttggcttt atcactcctg
       61 atgatggcag caaagacgtt ttcgtccatt tcaccgccat ccagagcaat gaattccgca
      121 cgctgaacga aaatcagaaa gttgaatttt ctattgagca ggggcaacgt ggccccgcgg
      181 cagcgaacgt tgttacgctc taaggttgcc gttattactc aacatctcca tttccgctgt
      241 ccatgttgtc atggttcaca gtaccgtaca tcggcattcg atgtgacgga gcgaaaccct
      301 ttgggcgcta agtgtatttt ttgtaaatcg actatgatca cctttgataa cgtcgcgctg
      361 caaatacgca ctgaccatgc gccgctggat ttcacaaaat aatatcaggc tccctcgtgg
      421 agcctttttt atatctgcct tatttttctt caacgctgta tgtatagtaa gcgataacct
      481 gttgattatt gaatctttcg ggaagatggc ttataacatt tcttacctga ccagggtacc
      541 gggaaccaac accttactgg cgtgttgctg tctttcaaga ccagacgagg ttaacagtga
      601 atattgaaga gttaaaaaaa caagccgaaa cggaaatcgc cgactttatc gcgcaaaaaa
      661 tcgccgagct gaacaagaat acagggaaag aagtctctga aattcgcttc atcgcacgag
      721 aaaaaatgac cgggcttgaa agttatgatg tcaaaatcaa aataatgtga ttttgtgaac
      781 atcaccccgt gcgaggtgat gttccgcttg ttgctaattt agtgaccaat cattggcgct
      841 tgtggaatta agcgtcggta caattcctcc ggcaccgggc tttgccatac tcccgcatac
      901 attgcgtaac caatcaccgc aaacataatc cccagaacca gtagcgtcat taaccagcca
      961 gacaacgcaa aggctttttt atttgctgca ggtttttgca gtgaaaaggt caatgttgag
     1021 gctaccggac atgactctac gcaagtcata cagccggtac attccactgt tcgtacctga
     1081 attaatttat ctaccgggat ccgtgatggg cacttttttg cgcatttgcc acaatcgata
     1141 caactttcgg cattgcgacg aatcttaaac ggcgacaata gcgaaaccac gcccatcagc
     1201 gcgccatatg ggcaaagata acgacaccag gcatggcgaa taaacaggct ggcaatcagc
     1261 aaaacggtca cgctgattaa tgtcgcggtc cccatatgac gaaagaaatc gagcatttta
     1321 acgtccatca ccacgctata gggcgacaac ataaaatagt gaatcgcctg agcgggcatc
     1381 aataacgcga tatagagaaa taaactcaac agcaaatact tcacgccgcg cagaggaata
     1441 tccagccagc ggggaaggac acattgccga ccaaacagtt tgttaccgag atcgccgatt
     1501 aattcagaaa gcgtaccaac cgggcataac catgagcaaa aggccttttt gagtaataga
     1561 ctgatgacga taaaagcgac caataacagc atcgcggcgg cgtggacgga cggtaactga
     1621 cctgttgcaa ggctatattt cagattcatc agaccggcaa tcggtagcca gccttcgata
     1681 cctcccggtc tggcgacaaa tgtcgtacta cctgccgttt cgtaatagcg cacccaatac
     1741 cagaacgtga tggcaatata aatattcatt gccaatagta agaattgcgt cgctttacgc
     1801 caggtcgtgg cattgcgcca gtcattccac ggtaatttgc cgcccgtcgt gcctggccgc
     1861 cgctgccagc gggttctttt tttctctgcc atgattttgc cagtccgtta agttgcatac
     1921 caaatgccac tattctagtt attcttaact ggctgatatt gattcaaatc gcgttcaggt
     1981 ctttcttatt caatcatgct tccagagcgg caacactgcg tgtaatttct tcgtgtggaa
     2041 ggggggcaga taatggctgc tgctccagtt gtgcgcatag ctgactggcg ctacgcattc
     2101 ccagactcga gcaactgctt tttagctgat gcgcggcacg ctttattttc tcgctgtcct
     2161 ggctggcgcg ggcaatgtcg atttcatcga gaagcggcag ggcatgttgt ttaaataatg
     2221 ccagccattc gtggatcttc tccgtcccca ttaactgagc gtcttcattg agttgcgaga
     2281 catccagtgg tagatcgtta ttggcttgca gttggagata gtgcgccagt aactgaccga
     2341 gcacttcacg cggcaccggt ttagggataa tcccgcgaaa tagtgaactg gtacgctggc
     2401 gcagcgtttc gtcaatgaca tgggcgctaa agccaatcaa aaccagcgac ggatattgct
     2461 gtgccagttg ccgggcaagg gtaatgccgt cgatatccgg cagatcaaaa tccaccagtg
     2521 cggcagcaaa cggttcgcta ttttgcagtg tctctaaagc ctgcgcggca ttgccaatag
     2581 caacaacctg cgcaccactg gtgttcagca tctcaacggt aattcgctgg gttagcgggt
     2641 tatcttcaat taacagcaaa cgtaaaccgt caagacgcac cgcctgattg gctgtttttg
     2701 gcacgggtgc agtggcaaca cgtaacggca agcgtaaaca aaaacagctt ccaacctccg
     2761 gcgtgctggt ggcgctcagt tcgccgccca tcgcctgggc cagacggcta ctgatagtca
     2821 gccccagccc ggtgccgccg cgtttgccgc ttacctgcac aaatggctgg aagatttctg
     2881 ccagtttcgc gggatcaata ccgcagccgc tgtcttccac ttcgaccagc cattgctcgc
     2941 catcagtgcg actacgcagg ataatgtgcc cttcgtcagt aaaacgcagg gcattgctca
     3001 ataggttggt aatgacctga cgaatacgtc gtggatcgcc cattaacgcg ctcggtacat
     3061 catcggcaat tgccgttgcc aggcgaatcg ggcgaccttt cacccgtccg ctcattaatt
     3121 gcagggtact ttccagcagc gggcgcggtt caaagggctc atcgctgact gaaacatttt
     3181 tgccacctgc ttcgatagcc gaataatcga gaatatcgtt gaggatggtc agcaacgatt
     3241 cgccagagtc ggtaatcgcc tgcaaatcat cacgctgggc gttaagtgcg gggttatctg
     3301 ccagcagttg agcagtgccg agaataccgt acagcggtgt gcggatctca tggctcatcg
     3361 ccgtcagaaa cgccgatttt gcctggctgg ctttttctgc ttccgcccgt gcctgtcggt
     3421 gttctatcac cagttcctgc aattcagctg tacgcgcttt gacctgcgcc gccagctgtt
     3481 cgcggtggcg attcagtgca tgaacactgc tgcgaaacgc atccatcagc cgcccgatgg
     3541 tatccagctc ccgtacgcca gcggtttccg ggaaagggga gtcaatatca ccgtccagca
     3601 gccgttgcag cgcctgcgtt tgttcggcaa gtggacgcgt gaatgagcga taaaccacgc
     3661 gccagaggat cataatcagt gcgcaaagtg aaaccatccc cagcaataac aggctgtatt
     3721 gcccgcgtgc actggctttt tccagatgtg ccagtccatg ctgattgtgc agctcaatgg
     3781 tgtcgaccag ctgactgact tcgctactaa actgcgcgaa ctgggcgata ttattttggg
     3841 cgagagtttg caggtgattg ctgatttcac tgtcctgctg gaacagcgcc aacagatcgc
     3901 tatattggct aacggtagtt aacgttgttg cgacctgcgc acgaactccc ggatcttcaa
     3961 tgcgtatttg ccgacgttgc agaattttca ccgcattatt gagctgcttt tccagcgttg
     4021 gtgcattttt ctggatctgc tccagcccca gattcatcac catttgctgc acccgcagag
     4081 cgctaaggcg cagttcattc atctggttaa catactcaag atcgatatca atcagccgat
     4141 cgagtgcact ttcagcagcc tgacgctgat cttgttcgat caaatcgtaa atcccggcct
     4201 gggtcgctcc agcggaagtt gtcgcattat tcgcctgacc ttgctccagg cgtgcgatct
     4261 catcggcggc agctatctgc tgactgagtt gccgttgttg ctggcgtagt tgcagacgcc
     4321 gccccaccag ttccccttgc tgacgtaatg aacgggagat ctcctgctcc tgttgttcaa
     4381 tagcggtggt atcaaaacct tgttcccgta acgcttgcag caacgcatta atcttcaggc
     4441 tttatgcggt gagcattcgc ccctgcgcct gctacatctt ttcgttatcg gcactggtca
     4501 ggttctgcgc ggcgaaaagt tcccaggcgc tggcttcgct caactggcgc gccatattca
     4561 tggtaggaat caatgcctga gtattgtctt tttccacctg gctgataaag cgcaggttgt
     4621 accatcccac cagggtactg gtcagggtta acagcgccat cagggcaaag cccatccaga
     4681 gtcttcgggt cagggttaaa ttcacggtcg gtgcacttaa ggtgaaaaag gttgagtcgc
     4741 aaagtggaat gcatctagca taaagcctta ttattgatga ggctatcatg cgcgtactgc
     4801 tatttttact tctttccctt ttcatgttgt cggcattttc ggctgataac ctgttgcgct
     4861 ggcatgatgc gcagcatttc acggtgcaag cctctatgcc gcttaaagcc aaacgcgtat
     4921 ggaaactgtg cgcgctttat cccagcctga aagattcata ctggttatcg ttgaactatg
     4981 gtatgcagga ggctgctcgc cgctacggtg tggatttaaa agtgctggag gcaggcggct
     5041 acagccagtt ggctacccag caagcacaaa tcgaccagtg taaacagtgg ggcgcagagg
     5101 ccattttgct cggtagtagc acgacgtcat ttcccgacct gcaaaagcag gtagcaaatc
     5161 tgccggtgat cgaactggta aatgctcttg atgatcccca ggtgaaaagc cgcgttggtg
     5221 taccctggtt tcagatgggc tatcaacctg ggcgatattt ggtgcaatgg gcgcacggta
     5281 aaccactgaa cgtgctgctg atgcccggac ccgataacgc cgggggcagt aaggagatgg
     5341 tcgagggttt tcgcgcagcc attgccggaa gcccggtgcg tattgttgat attgcgcttg
     5401 gtgataacga tattgaaatc cagcgcaacc tgttgcagga gatgctggaa cgccacccag
     5461 aaatcgacgt cgttgccgga acggccattg cggcagaggc ggcaatgggt gaagggcgta
     5521 acctgaaaac gccgcttacc gtggtgtcgt tttatctttc acatcaggtg tatcgcgggc
     5581 tgaagcgggg aagagtgatt atggctgcca gcgatcaaat ggtctggcag ggggaactgg
     5641 cagttgagca ggccatcagg caattacagg ggcaatcggt gtctgataat gtcagcccac
     5701 cgattttagt tctgacgccg aaaaatgccg accgagaaca tatccgccgc tcgctgtcac
     5761 cggggggatt tcgtccggtc tatttttatc agcacacatc agcggctaag aaataacctt
     5821 caccatgttg cgtcaccagt aaatccgcgc tgagtttatg acgtaaacga cgaattaaca
     5881 catcgacggt gcgcaggtca gggttttcca cccgacgcgc agaaagcata cgcagcagac
     5941 gctcacggct gagaatttcg cccggattag tcacaaatgc caccaacatt tcatactctg
     6001 cgcgggtcag tttaatcggc tcgccatccc gctccagcgt atggcgcgac acattcaggc
     6061 aataacccgc aaagcgatag cagtagtcct gagtgtgcgg ttgagcttgt cgcgcgaggt
     6121 cgattcgcca gagcagattt ttcacccgta ctaccagttc gcgcagttcc agcggtttgg
     6181 tgacgtaatc gtctgcgccc atttccagcc caacaatacg gtcaatccga tcgctgcgtc
     6241 cggtaaccag aataatcccc accgttgagc gttctcgcag ggcgcgggtt aacatcaggc
     6301 cattttcatc gggtaagttg atatccagca gaattaaatc taccggctga ttctgcataa
     6361 tttcccgtag cccggcaccg ctcgccgtaa cggaaacggt atacccctcc tgagtgaagt
     6421 aggattgtaa tcgcgcctgg gtaaccggct tatcttcaac aataacaatg tgatgtggca
     6481 tcagagggtt ttactcattc tgttcatatc tgttcatatt tgctcattaa gatcgcttca
     6541 ctaaaccata attctacagg ggttattatg cggaaactct ggaacgcgct acgccgaccc
     6601 agtgctcgtt ggtcggtact ggcgctggtc gcaattggga ttgtgattgg cattgcgctg
     6661 attgtattgc cacacgttgg gatcaaagtc accagcacaa ccgaattttg tgtcagttgc
     6721 cacagtatgc aaccggtgta tgaagaatat aaacagtcgg tgcatttcca gaacgcctcc
     6781 ggcgtgcgag ctgaatgcca tgactgtcat atcccgccgg atattccagg catggtgaag
     6841 cgcaaactgg aagcgagcaa cgacatttac cagaccttta ttgcccactc cattgataca
     6901 cctgaaaaat tcgaagccaa acgcgcggaa cttgccgagc gtgaatgggc gcgaatgaaa
     6961 gaaaacaact cggcaacctg tcgctcctgc cataactacg atgcgatgga tcatgccagg
     7021 cagcatcctg aagcggcacg tcagatgaag gtggcagcga aagataatca atcctgcatc
     7081 gactgtcata aaggtattgc ccaccagtta ccggatatga gtagcggctt ccgtaagcag
     7141 ttcgatgagc tgcgcgccag tgctaatgac agtggtgaca cgctgtactc tattgatatt
     7201 aagccaattt atgcggcgaa aggcgataaa gaagcctctg gttctctgct gcctgcttcg
     7261 gaagtgaaag tccttaaacg tgacggcgac tggctgcaaa ttgaaattac cggctggacg
     7321 gaaagcgccg gacgtcagcg tgtactcacc caattcccag gtaaacgcat ctttgttgcc
     7381 tcgattcgtg gtgatgtgca gcagcaggtg aaaacgctgg agaaaaccac cgttgccgac
     7441 accaataccg agtggagcaa gttgcaggcc accgcgtgga tgaagaaagg cgacatggtg
     7501 aacgatatca aaccgatctg ggcctatgcg gattcgttgt acaacggcac ctgtaacgag
     7561 tgccacggcg caccggaaat cgcccacttt gacgctaacg gttggatcgg cacgctcaac
     7621 ggcatgattg gctttaccag tctcgataaa cgtgaagaac gcactttgtt gaaatatctg
     7681 caaatgaatg cgtctgacac cgcaggtaag gctcacggcg ataagaagga agaaaaataa
     7741 tgaacaataa cgatctcttt caggcatcac gtcggcgttt tctggcacaa ctcggcggct
     7801 taaccgtcgc cgggatgctg gggccgtcat tgttaacgcc gcgccgtgca actgcggcgc
     7861 aagcggcgac tgaggctgtc atctcgaaag agggcattct taccgggtcg cactgggggg
     7921 ctatccgcgc gatggtgaag gatggtcgct ttgtggcggc aaaaccgttc gaactggata
     7981 aatatccgtc gaaaatgatc gccggattgc cggatcacgt acacaacgcg gcacgtattc
     8041 gttatccgat ggtacgcgtg gactggctgc gtaagcgcca tctgagcgac acctcccagc
     8101 gcggtgataa ccgttttgtg cgcgtgagct gggatgaagc cctcgacatg ttctatgaag
     8161 aactggaacg cgtacagaaa actcacgggc cgagtgcctt gctgaccgcc agtggttggc
     8221 aatcgacggg gatgttccat aacgcttcgg ggatgctggc gaaagctatt gccttgcatg
     8281 gtaatagcgt tggtacgggc ggagattact ctaccggtgc tgcgcaggtg atcctgccgc
     8341 gcgtagtcgg ttcaatggaa gtgtatgaac agcaaacctc ctggccgctg gtattgcaga
     8401 acagcaaaac cattgtgctg tggggctccg atttgctgaa aaaccagcaa gcgaactggt
     8461 ggtgcccgga tcacgatgtt tatgaatatt acgagcagct aaaagcgaaa gtcgccgccg
     8521 gtgaagttga ggtcatcagc atcgatccgg ttgtcacatc cacccatgag tatctggggc
     8581 gcgagcatgt gaagcacatt gcggttaacc cgcaaactga cgtgccgctg caactggcgc
     8641 tggcacatac gctgtacagt gaaaacctgt acgacaaaaa cttcctcgct aactactgtg
     8701 tgggttttga gcagttcctg ccgtatctgc tgggtgagaa agacggtcag ccgaaagatg
     8761 ccgcatgggc tgaaaaactg accggcattg atgccgaaac cattcgtggg ctggcgcggc
     8821 agatggcggc gaacagaacg caaattattg ctggctggtg cgtgcagcgt atgcagcacg
     8881 gtgaacagtg ggcgtggatg attgtggttc tggcggcgat gctggggcaa attggcctgc
     8941 caggtggtgg ttttggctgg cactacaacg gcgcaggcac gccggggcgt aaaggcgtta
     9001 ttctgagtgg tttctccggc tctacgtcga ttccgcctgt tcacgacaac agtgactaca
     9061 aaggctacag cagcactatt ccgattgccc gttttatcga tgcgatcctc gaaccgggga
     9121 aagtgatcaa ctggaacggt aaatcggtaa aactgccgcc gctgaaaatg tgtatttttg
     9181 ccggaactaa cccattccat cgccatcagc agatcaaccg cattattgaa ggctggcgca
     9241 agctggaaac ggttatcgcc atagataacc agtggacctc aacctgccgc tttgccgata
     9301 tcgtgctgcc tgcgaccacg cagtttgagc gtaacgatct cgaccagtac ggcaaccact
     9361 ccaaccgtgg cattatcgcc atgaaacagg tggtgccgcc gtagttcgag gcgcgcaacg
     9421 actttgatat tttccgcgag ctgtgccgtc gctttaatcg tgaagaagcc tttaccgaag
     9481 ggctggacga aatgggctgg ctgaaacgca tctggcagga aggtgtacaa caaggcaaag
     9541 gacgcggcgt tcatctgcca gcgtttgatg acttctggaa taacaaagag tacgtcgagt
     9601 ttgaccatcc gcagatgttt gttcgccacc aggcattccg cgaagatccg gatctcgaac
     9661 cgctgggcac gccgagtggc ctgattgaga tctactcgaa aaccatcgcc gatatgaact
     9721 acgacgattg tcaggggcat ccgatgtggt ttgagaaaat cgaacgctcc cacggtgggc
     9781 ccggctcgca gaagtatccg ttgcatctgc aatctgtgca tccggatttc cgacttcact
     9841 cgcagctatg tgagtcggaa acgctgcgtc agcaatatac ggtagcgggt aaagagccag
     9901 tgttcattaa cccgcaggat gccagcgcgc gcggtattcg taacggtgat gtggtacgcg
     9961 tctttaacgc tcgcggtcag gtgttggcag gagcagtagt ttctgaccgc tatgcacccg
    10021 gcgtggcgcg aattcacgaa ggggcatggt acgatccaga taaaggcggc gagcctggtg
    10081 cgctgtgcaa atacggtaac cccaacgtgc tgaccatcga catcgggact tcgcagctgg
    10141 cgcaggcgac cagtgcgcac actacgctgg tggaaattga gaagtacaat ggaacagtgg
    10201 agcaggtgac ggcgtttaac ggccccgtgg agatggtggc gcagtgcgaa tatgttcccg
    10261 cgtcgcaggt gaaatcatga ccacgctgac agcacaacag attgcctgtg tttacgcctg
    10321 gctggcgcag ttgttctccc gtgaactgga cgatgaacaa ctgacgcaaa tcgccagtgc
    10381 gcagatggct gaatggtttt cgttgctgaa aagcgaaccg ccgctcactg cggcggtgaa
    10441 tgatctggaa aacagtattg ccac
//
LOCUS       DAJTSA010000127        10105 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000127 DAJTSA010000000
VERSION     DAJTSA010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.127"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            123..254
                     /locus_tag="OO369_003547"
     CDS             123..254
                     /locus_tag="OO369_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR5983679.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            514..1509
                     /gene="pgl"
                     /locus_tag="OO369_003548"
     CDS             514..1509
                     /gene="pgl"
                     /locus_tag="OO369_003548"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR5983680.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1550..2503)
                     /locus_tag="OO369_003549"
                     /pseudo
     CDS             complement(1550..2503)
                     /locus_tag="OO369_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2687..3739
                     /locus_tag="OO369_003550"
     CDS             2687..3739
                     /locus_tag="OO369_003550"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR5983681.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3815..5248
                     /locus_tag="OO369_003551"
     CDS             3815..5248
                     /locus_tag="OO369_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCR5983682.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5431..7611
                     /locus_tag="OO369_003552"
     CDS             5431..7611
                     /locus_tag="OO369_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR5983683.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7752..9035)
                     /locus_tag="OO369_003553"
     CDS             complement(7752..9035)
                     /locus_tag="OO369_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR5983684.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(9170..10051)
                     /locus_tag="OO369_003554"
     CDS             complement(9170..10051)
                     /locus_tag="OO369_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5983685.1"
                     /translation="MTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAI
                     RRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPV
                     AATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSD
                     IVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLS
                     SGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMA
                     SQYRDDRGREWDKIEIK"
BASE COUNT         2452 a         2358 c         2552 g         2743 t
ORIGIN      
        1 tttttttctt taaaaaaaca gtacattcta ttttataatg actgtgtatt cccatggaag
       61 gagatcaccc aattctcccc cccgactttt gacgcagtgt cttgttaaca gggggattga
      121 ccatgcttat tggctatgta cgcgtatcaa caaatgacca gaataccgat ctacaacgta
      181 atgcgtttat ttgtgccgga agtgagttga ttgttgcgcg aatgggaagt tggccagaag
      241 ttgttatggc atagtgttta cggtcgttga tacaggtaaa caaaagcggg gagagtcata
      301 ctccctgaat agtcgcgaaa atatctgtgg cattgtcaaa ttcgtgtcgg tgactaatat
      361 tttagtaata tgccattcga aatggagtgc taacttattg atttaattga tgtgcgatat
      421 tgtattttat cgtgttttgt tgtctgtttt ttatccagta agtgttgtta tatgtatgaa
      481 agataaggga tttatcattt ttgaggtgtg ttcatgaagc aaacagttta tatcgccagc
      541 cctgagagcc agcaaattca cgtctggaac ctgaatcatg aaggcgcact gacgctgaca
      601 caggttgtcg atgtgccggg gcaggtgcag ccgatggtgg tcagcccgga caaacgttat
      661 ctctatgttg gtgttcgccc tgagtttcgc gtcctggcgt atcgtattgc cccggacgat
      721 ggcgcactga cctttgccgc agagtctgtg ctgccgggta gtccgacgca tatttccacc
      781 gatcaccagg ggcagtttgt ctttgtaggt tcttacaatg cgggtaacgt gagcgtaacg
      841 cgtctggaag atggcctgcc agtgggcgtc gtcgatgtag tcgaggggct ggacggttgc
      901 cattccgcca atatctcacc ggacaaccgt acgctgtggg ttccggcatt aaagcaggat
      961 cgcatttgcc tgtttacggt cagcgatgat ggtcatctgg tggcgcagga ccctgcggaa
     1021 gtgactaccg ttgaaggggc cggcccgcgt catatggtat tccatccaaa cgaacaatat
     1081 gcgtattgcg tcaatgagtt aaacagctca gtggatgtct gggaactgaa agatccgcac
     1141 ggcaatatcg aatgtgtcca gacgctggat atgatgccgg aaaacttctc cgacacccgt
     1201 tgggcggctg atattcatat caccccggat ggtcgccatt tatacgcctg cgaccgtacc
     1261 gccagtctga ttaccgtttt cagcgtttcg gaagatggca gcgtgttgag taaagaaggc
     1321 ttccagccaa cggaaaccca gccgcgcggc ttcaatgttg attacagcgg caagtatctg
     1381 attgccgctg ggcaaaaatc tcaccacatc tcggtatacg aaattgttgg cgagcagggg
     1441 ctactgcatg aaaaaggccg ctatgcggtc gggcagggac caatgtgggt ggtggttaac
     1501 gcacactaac cgctgattta accggcgcag tctctcctgc gccggtgtat taacttatct
     1561 cctgtaacgc atgtctctgg cgttcgacga tattggtcca caaattgtct ttatcgtcag
     1621 tccacaaatt aatcagcaag gaaaaaaagc gttcagctgc cggagaaagc acggcatctt
     1681 tgcggcgaat aatccccaat gtccgacgta taacgggttc caccagtggg ataccaataa
     1741 tcgaagaata gggcgcatgg ggcattgcca gaccaggaag cgccgaaatc cccagtcctg
     1801 cctccactag tcctaatgac gtcgaaagat gacgcacttc gtaaaaccag tccagcttcc
     1861 agggcttgtc ggctagctgt tgttctatca acaatcggtt gccgctggag gaacgtacgc
     1921 caatcatttt gtaacccact aactcctgcc attctacaag ctactttttg gccaatggat
     1981 ggtcacgccg acaggccagc acgaatggct cgttgaccag tggggtaaaa tcaatggatg
     2041 aatttgtgac gttgttcatg tttatgccaa agtctgattc gttacacagc accgattcca
     2101 tgcaattatt cgtgccttgt tccagaatcc gcactttaat attgggatac agctcattaa
     2161 atttaccgat tgccagcggt aaaaaataaa atactgcggt cggaatacac gctaatgtca
     2221 ccataccacg atgataagca ttcatatcac gaatattaaa aagcgtttca tcgaatttct
     2281 ttattaattc ccgcgcctcc gggagcaacc tttttcctgc ttttgttaac gtaaccttgc
     2341 gagttgtacg ctcaaatagt tgaatatgta aatcttcctc catctttttt attcttcgcg
     2401 ttaatgcagg ttgcgtaata ttgagtaatt tagcggcatt gttaaatgaa ctggactctg
     2461 ccagtatgac aaatgccttc atacttgata attcatgctt cattattact ccggaaaatg
     2521 gaagcgacga ttttgggggg ctggtcgtta aaaattttaa ctgcatttag ccaacttaaa
     2581 ttaatgaaaa aatgttatta atcgttgagc taaagtcatt agagatgctt tgtccttaat
     2641 gtaaccatat cgcaataagt tatgttttta aattgagggc attattatga aaaaaatacc
     2701 ctgcgtgatg atgcgaggtg gaacctcgag gggcgcgttc ctgttagcgg aacatttacc
     2761 cgaagatcaa acgcagcgcg ataaaatatt gatggcaatt atgggttccg gtaacgatct
     2821 ggaaattgac ggtattggcg gcggtaatcc actgaccagt aaagtcgcca ttattagccg
     2881 ttccagcgat ccgcgtgctg atgtcgatta tctgtttgct caagttatcg tccatgagca
     2941 acgtgtcgat accacgccta actgcggcaa tatgctgtct ggcgtagggg cattcgccat
     3001 tgaaaatggt ttgattgcag cgacttcgcc agttacccgt gtacgtatcc gcaacgtcaa
     3061 tacgggtacg ttcatcgaag ctgatgtgca aacgccaaat ggtgttgtcg agtacgaggg
     3121 tagcgccaga attgacggcg taccgggtac tgccgcaccg gttgcgctca ctttcctgaa
     3181 tgccgctgga acgaaaactg gaaaagtttt cccgactgat aatcagattg attattttga
     3241 cgatgtcccg gtgacctgta tcgatatggc gatgccagtc gtcattattc cggctgaata
     3301 tctgggaaaa acaggttatg aattaccggc ggaactggat gccgacaaag cattattagc
     3361 ccgcattgaa tctatccggc tacaagcggg taaagcaatg ggcttaggtg atgtcagtaa
     3421 tatggttatc cctaaacctg tgcttatttc tccagcgcag aaaggcgggg caattaatgt
     3481 gcgttatttt atgccgcatt cttgccatcg cgcgctggcg ataaccggtg ctattgctat
     3541 ttccagtagt tgtgcattgg aaggcaccgt cacccgacaa atcgtccctt ctgtaggata
     3601 tggcaatatc aatattgaac accccagtgg tgcgctcgac gttcatttaa gtaatgaagg
     3661 tcaggatgcc acgacgttac gcgcatctgt tattcggaca accagaaaaa tattttccgg
     3721 tgaagtttat cttccctgaa aaaattcgtt gtcaggataa ggacaatcaa taaaggactt
     3781 ctgtatgagt catacagaaa gaacaggatt ttaaatgaat aagaaatcgt tatggaagct
     3841 aattctgata ttagcgatcc catgtattat tggttttatg ccagctccgg caggattaag
     3901 cgaactggcg tgggtgcttt ttggtattta cctggcggcc attgtgggtc tggttatcaa
     3961 gcctttcccg gaacctgtcg tactgttaat tgccgttgct gcctcaatgg tggtggtcgg
     4021 taacttatcc gacggtgcgt ttaagaccac cgccgtatta agcggttact cttcaggtac
     4081 tacctggctg gtgttctcgg cgtttacctt aagcgccgca tttgtgacca ccggtttagg
     4141 taaacttatt gcctatctgc tgattggtaa aatcggtaac accatgctgg gtctgggtta
     4201 cgttacggta ttcctcgatc tggtactggc tccggcaaca ccgtctaaca ccgcgcgtgc
     4261 gggcggcatt gtgttaccga tcatcaacag cgtggcggtg gctttggggt ccgaaccgga
     4321 aaaaagtccg cgtcgtgtcg gacattacct gatgatgtcc atttacatgg tcaccaaaac
     4381 caccagctat atgttcttta ccgcaatggc ggggaacatt ctggcgctga aaatgatcaa
     4441 cgacattctg cacctgcaaa ttagctgggg tggatgggcg ctggccgccg gattgccggg
     4501 catcattatg ctgctggtca ctccgctggt aatttacacc atgtatccac cagaaattaa
     4561 aaaggtggat aacaaaacca tcgctaaagc gggccttgcc gaactgggac cgatgaaaat
     4621 ccgcgaaaaa atgctgctcg gtgtctttgt gctggcgcta ctgggctgga ttttcagtaa
     4681 gtctctgggg gttgatgaat ccactgtggc aatcgttgtt atggcgacca tgctgctgct
     4741 gggtatcgtt acctgggaag acgtggttaa aaataaaggc ggctggaata ccttaatctg
     4801 gtacggcggt attatcggct taagctcctt attatcgaaa gttaaatttt tcgaatggtt
     4861 agctgaagtc tttaaaaata acctggcatt tgatggtcac ggtaacgttg ctttcttcgt
     4921 tattattttc ctcagcatca tcgtgcgtta tttcttcgct tccggtagtg cctatatcgt
     4981 tgctatgtta cctgtatttg ccatgctggc gaacgtctcc ggcgcaccgt taatgttaac
     5041 cgcgctggca ctgttgttct ccaactccta tggcggcatg gttactcact atggcggcgc
     5101 ggcaggtccg gtcatctttg gcgtgggtta caacgatatt aaatcctggt ggttggtcgg
     5161 tgcggtactg acgatattaa ccttcttggt gcatatcacc ctcggcgtgt ggtggtggaa
     5221 tatgctgatc ggctggaaca tgctgtaaat atactcgtca tacttcaagt tgcatgtgct
     5281 gcgtctgcgt tcgctcaccc cagtcactta cttatgtaag ctcctgggga ttcactcact
     5341 tgtcgccttc ctgcaactcg aattatttag agtatatcca tttattatct ttctgcgcac
     5401 ttcacggtgc gcagatatct ggagcatttg atgatcaagt tatctgaaaa aggcgtgttt
     5461 ctcgccagta ataacgaaat aattgccgaa gaacatttca ccggcgaaat taaaaaagaa
     5521 gaagcaaaaa aaggcactat tgcctggtct attctctctt ctcataatac gtccggaaat
     5581 atggataaac ttaaaattaa gtttgattca ttagcctctc acgatattac ctttgttggt
     5641 attgtacaga ccgctaaagc gtccggaatg gaacgtttcc cgctgccgta tgtgctgacc
     5701 aactgccata actcactctg cgccgtcggc ggcaccatta acggtgatga ccatgttttt
     5761 ggtttatcgg cagctcagcg ttatggcggt atttttgtgc ctccgcatat tgcggtcatc
     5821 catcaatata tgcgtgagat gatggctggc gggggcaaaa tgatcctcgg gtcagacagc
     5881 cacacccgtt acggtgcatt agggacaatg gcagtcggtg agggtggcgg tgagttggta
     5941 aaacagctgc ttaatgacac ctgggatatc gactatccgg gcgtggttgc ggtgcatctg
     6001 accggaaaac ctgcgccgta tgtggggcca caggatgtgg cgctggctat cattggcgcg
     6061 gtgttcaaaa acggttacgt caaaaacaaa gtcatggagt tcgttggacc gggcgttagc
     6121 gcgctctcta ccgatttccg taacagcgtt gacgtgatga ccacggaaac cacctgttta
     6181 agctccgtct ggcaaactga tgaagaagtg cataactggc tggcgctgca cggtcgcggt
     6241 caggattact gccagcttaa ccctcaaccg atggcgtact acgatggctg catcagcgtt
     6301 gatttaagcg ccatcaaacc aatgattgcg ctgccgttcc acccgagcaa cgtgtatgaa
     6361 atcgacacac tgaaccagaa cctgaccgac attctgcgtg agattgaaat tgagtccgaa
     6421 cgcgtggcgc acggtaaagc caaactctcg ctgctggata aagtggaaaa tggtcgcctg
     6481 aaagtgcagc aggggattat cgcgggctgt tctggcggta actacgaaaa cgtcatcgcg
     6541 gcggcgaatg cactgcgcgg tcaatcctgt ggcaatgaca ccttctcgct ggcggtttac
     6601 ccgtcatcac agccggtgtt tatggatctc gccaaaaaag gtgtggtagc agatttgatt
     6661 ggcgcaggcg caatcatcag aaccgcattc tgtggcccat gctttggcgc gggcgatacg
     6721 ccaatcaaca acggtttgag tattcgccac accacgcgta acttcccgaa ccgcgaaggc
     6781 tctaagccag ccaatgggca gatgtcagcg gtggcgttga tggacgctcg ttctatcgct
     6841 gcgactgcgg caaacggtgg ctatttaacc tctgccagcg aacttgattg ctgggacaac
     6901 gtgccggagt acgccttcga tgtaacgccg tataaaaacc gtgtttatca gggctttgtg
     6961 aaaggggcaa ctcagcaacc gctgatttac ggaccgaaca ttaaagactg gccggaattg
     7021 ggtgcgctga ctgacaatat cgtcctgaaa gtgtgctcga agatcctcga cgaagtcacc
     7081 accaccgacg aactgattcc ttccggtgaa acctcttctt atcgttcaaa tccgattggt
     7141 ctggcgggga atgtcagcga gctgacagag gtgtttgcgc gcattaagca gattgctggt
     7201 caggagcata ttgatccgct gcaaactgaa attggcagca tggtctatgc ggtgaaacca
     7261 ggcgatggtt ctgcgcgtga acaggcggcg agctgccagc gtgtgattgg cggtctggcg
     7321 aatattgccg aggagtacgc gactaaacgc taccgttcta acgtcatcaa ctgggggatg
     7381 ttaccgctgc agatggcgga agtgccaacc tttgaagtgg gggattacat ttacatccct
     7441 ggcattaaag cggcgctgga taatccgggt acgacgttta aaggttatgt gatccatgaa
     7501 gatgcgccgg taacggaaat tacgctttac atggaaagcc tgacggctga agagcgcgag
     7561 attatcaagg cgggtagttt gattaacttc aataaaaacc gtcagatgta aaaagcgcca
     7621 tgtgaatgta ggtcgcattc ggcacttatt gtcggatgcg atgctggcgc atcttatccg
     7681 acctacaaat cgcatcgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcaaata
     7741 gttaatagct cttacttctt ctcctctgca accactttgc tgcccacgcc gcggttattg
     7801 tattcccaca tgcggttgta gttagtgtca ttcagattgc gctgtatttc gtcgttatca
     7861 tctacgctgc cggtattacc cgcaaacgga cgattagaga tcaccgcatc ggcccacggt
     7921 ttagccgtgt taaaaccttc gttgatggcg ctatcacgga tcaccacctg accgttggta
     7981 ttagcatcaa catccagcga gcggcccagt tgcgccacgc catcaccgga agcattgaaa
     8041 cggctgttta cggcgaggaa accgtaatag atgttagaca gcgtagccgg tgcaaacaca
     8101 tatgcttctt gctgggtacg tgagttcacc acgcggaatt cggtgttatc gaacaccact
     8161 gcgccgcgac cagaaacgat atccacatcc ccttcaatat agctgttggt caccagcgta
     8221 cgcggctgac ggttggtttc cagacggttc tgcacaccgc tgttggtgac aaagaaggtg
     8281 ttctgacgac cgagaatgtt aacgttgtta atctgtactt ggtcaccgtc agtacgcagt
     8341 gctaccgccg gatggttacc tgcatctacg ctatcgccca gcgtgttttc gatggtcaga
     8401 ttttgcagtt gcagtccatt gttttgtgac cagaagaccg cagaacacag aacaccgata
     8461 ctgtcgctgc gtttgctctg gcagctatcg tacatatacc acgctggttt acctggcata
     8521 tatttgccgc gcgggttgac gtcgtgacgc cagtcggcag ggctcatgcc cccatcaagg
     8581 gaaagcccaa tcttcacatc aatcggtttt tcacctgtac cgtacagagt gattccacct
     8641 ggagcagcag ggacatacac cgttccctga tactcaccag gcatcacggc aatatactgg
     8701 cgcttgttgg tgcgcttgat aattgccgca tctaccgccg cctgaatcgt ggtatgcgtt
     8761 acaccttgag tacccgccgg gccgacaaca aagtcaggtt gcgcaggcag ggttatcggg
     8821 gacggattcc acgctgcagc acctggtgtc agggatgcaa aatagtgttg cgcatcgaaa
     8881 ttcagcgctt cttttgccga cagaatcggg cgagaagagg taccaggcgc agtttgatca
     8941 gaaggacgtt gatcgggtgg tgttgagcta caggcggtca gcgtcacgcc aaaagccaat
     9001 gccagcgcca gacgggaaac tgaaaatgtg ttcacaggtt gctccgggct atgaaataga
     9061 aaaatgaatc cgttgaagcc tgctttttta tactaagttg gcattataaa aaagcattgc
     9121 ttatcaattt gttgcaacga acaggtcatt atcagtcaaa ataaaatcat tatttgattt
     9181 caattttgtc ccactccctg cctctgtcat cacgatactg tgatgccatg gtgtccgact
     9241 tgtgcccgag aagatgttga gcaaacttat cgcttatctg cttctcatag agtcttgcag
     9301 acaaactgcg caactcgtga aaggtaggtg gatccccttc gaaggaaaga cctgatgctt
     9361 ttcgtgcgcg cataaaatac cttgatactg tgccggatga aagcggttca cgacgagtag
     9421 atgcaattat ggtttctccg ccaagaatct ctttgcattt atcaagtgtt tccttcattg
     9481 atatcccgag agcatcaaca tgcaatgttg tagggatggc aatttttacg cctgttttgc
     9541 tttgctcgac ataaagatat ccatctacga tatcagacca cttcatttcg cataaatcac
     9601 caactcgttg cccggtaaca acagccagtt ccattgcaag tctgagccaa catggtgatg
     9661 attctgctgc ttgataaatt ttcaggtatt cgtcagccgt aagtcttgat ctccttacct
     9721 ctgattttgc tgcgcgagtg gcagcgaccg ggtttgttgt tatatggcct tcagctattg
     9781 cctctcggaa tgcatcgctc agtgttgatc tgattaactt ggctgatgcc gccttgccct
     9841 cgtctatgta tccattgagc attgccgcaa tttcttttgt ggtgatgtct tcaagtggag
     9901 catcaggcag ccccctcctt attgctttaa ttttgctcat gtaatttatg agtgtcttct
     9961 gcttgattcc tctgctggcg aggatttttt cgtagcgatc aagccatgaa tgtaacgtaa
    10021 cagaattatc actgttgatt ctcgctgtca gaggcttgtg tttgtgtcct gaaaataact
    10081 caatgttggc ctgtatagct tcagt
//
LOCUS       DAJTSA010000128         9934 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000128 DAJTSA010000000
VERSION     DAJTSA010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9934
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.128"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="OO369_003555"
     CDS             complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="OO369_003555"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR5983686.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVG"
     gene            complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="OO369_003556"
     CDS             complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="OO369_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR5983687.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(3052..3873)
                     /gene="bglG"
                     /locus_tag="OO369_003557"
     CDS             complement(3052..3873)
                     /gene="bglG"
                     /locus_tag="OO369_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR5983688.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(4160..4885)
                     /gene="phoU"
                     /locus_tag="OO369_003558"
     CDS             complement(4160..4885)
                     /gene="phoU"
                     /locus_tag="OO369_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR5983689.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4900..5673)
                     /gene="pstB"
                     /locus_tag="OO369_003559"
     CDS             complement(4900..5673)
                     /gene="pstB"
                     /locus_tag="OO369_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR5983690.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5765..6655)
                     /gene="pstA"
                     /locus_tag="OO369_003560"
     CDS             complement(5765..6655)
                     /gene="pstA"
                     /locus_tag="OO369_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR5983691.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6655..7614)
                     /gene="pstC"
                     /locus_tag="OO369_003561"
     CDS             complement(6655..7614)
                     /gene="pstC"
                     /locus_tag="OO369_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR5983692.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7701..8741)
                     /gene="pstS"
                     /locus_tag="OO369_003562"
     CDS             complement(7701..8741)
                     /gene="pstS"
                     /locus_tag="OO369_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR5983693.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8989..>9934)
                     /locus_tag="OO369_003563"
     CDS             complement(8989..>9934)
                     /locus_tag="OO369_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5983694.1"
                     /translation="GYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYY
                     TSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYN
                     CNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADV
                     YIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGI
                     LNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVY
                     GEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         2561 a         2586 c         2294 g         2493 t
ORIGIN      
        1 tccaaccgga tcaatttgcc acccccactc tgaacttttc agatgcggat tggggatcat
       61 attcagtatg ttgccctgcg catttttatt aatgctttcg tcgtgggaaa cacaaccagt
      121 catgtaataa ctaaaagaga tgaaatctac agtatgtttt aaatcttctg cgtcactttc
      181 agtcatctca atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc cgggatattg
      241 gccacgcgcc tgaacatcac caaagaacat ccagcgccgg ttctcttcca tggcctgcaa
      301 catatcctgt ggctggcagg tgagggggta aaccagccca ccgagaagca tattgccgat
      361 tttcgcttcg gggatcaggc tatgataggc tttaactgcc cgcgcactgg caaccagttg
      421 atgatggatc gcctgataaa cttccgcctc gccactctct tctgccagcc ccacgcccgt
      481 gaatggcgcg tgtaacgaca tattgatttc attaaacgtc agccataacg ctactttatg
      541 ttggtagcgg gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga ccgcccgatt
      601 agcccaaccg ccgtagtttt tcaccagccc atatagcatt tcgtaatggg ataacgttac
      661 cagcggcttg atccccgcct gcgccatttc atcaaacagc cgatcgtaaa acgctaaccc
      721 cgcttcattc ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg caatggaaat
      781 acgcagacag gtgaagccca tctcggcaaa taacgcgata tcttccgggt aacggtaata
      841 aaaatcgatg gcgacatctt tgatattctc tttccccagg atgcgcggtt ccatttttcc
      901 cattacgcca tgaggctgta aatctgaggt cgtgatccct ttgccatctt ccagccaggc
      961 accttccacc tgattggcag ctgttgcgcc accccaaaga aatgtttctg gaaatgcttt
     1021 cataattaac tccttttatc gttagcgaat gatggataac agcggttcac ctgcgcttat
     1081 ctgcgccgtg ccgtggggta atacgtctgt aaaatcatcg ctattactga ttaataccgg
     1141 cgtcgtcaga tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa tcagccgatc
     1201 gcctgtattg accttgtcac ccacgttgac gtgagcggaa aagaatttgc cgtccagttt
     1261 tacggtgtcg ataccgacat gaatcaggat ctccacacca tcatctgact caatgccaat
     1321 ggcgtgtaat gtggcgaaca acgaagcaat tcgacccgca accggagaac gcacttcacc
     1381 aaccgagggc agaatggcga tacctttacc caacaggcca ctggcaaacg tggtatcagc
     1441 gacgtgaatg agcggcacaa tctctcccgt catcggtgaa cagataccgc cctgctcagg
     1501 tggtgtaata acctctggtg ttttctcttg cggggcaccc tgcgctggct gacgtttact
     1561 ggtgatgaaa tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa tgacaccgcc
     1621 aataacgctg gcccagacgg tgaaatcaat ccccgttgac gggatggtct gcatgaaggt
     1681 gaaaatactt ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa tgatggtggc
     1741 ccccaatgct ccactgatac aggcgataac aaaggggtac ttacgcggca ggttgacgcc
     1801 atataccgct ggttcggtga taccaaacag acccgtcaac gccgctgatc ccgccaccac
     1861 ttttttctgc gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga cctgcgccat
     1921 aatggcgggc attaacagcg ggatcatggt gtcgtagccc agcacggtga agttattgat
     1981 acacagcggc accaggcccc tgtgcagtcc gaacatgacg aagatttgcc agaagccgcc
     2041 cattaccgcg cccgcaaatg caggaaccgc ctgataaagc cagagataac cggcggcaat
     2101 catttcgctt atccaggttg atagctgccc caccagcaga aaggtgacgg gtgtgataac
     2161 catcagacat agcaatggtg tgaagaaatt tttgattgcc gacggtaacc acgcattaag
     2221 tcggcgttcc agaatgctgc acaaccaggc agaaaaaata atgggaataa ccgatgacga
     2281 gtaattcaac aatgtgaccg gaatacccag gaaatccagc cccggcgcat ccgctttttg
     2341 cccgttctcg aaggcagtca gaattaatgg atgcactaac gctccaccaa tcaccatggc
     2401 agtaaatgga ttaccgccga agcgtttgcc cgcggtgtat cccaggatta tcgggaagaa
     2461 cccaaacaag gcatcactgg cgctgaataa aattaaataa gtaccacttt gttcggtcgt
     2521 ccactgaaaa gtgagcgcca gagccagcat acctttcagg atcccggttg ccgccatcag
     2581 accgatcaga ggcgtaaaaa tacctgaaat aacataaaca aagcggttta gcagattacc
     2641 tttctcatca ttttccggtg cctgttgcgc tttttcgcca aggcctgcca cgctgttaac
     2701 cgccaggaag acatcggcca catggttacc tatgaccacc tgaaactgac caccgctttc
     2761 caccaccata ataataccgg gggtcttttt cagtacctca gcttgcgctt tgctttcatc
     2821 ctttaactta aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt tatctgcgcc
     2881 cccgactcct gcgactattt ttctggctaa ctccgtcata acttgccctc taccgctttg
     2941 cggcaaaact ccaaaaaaaa cctgaaaaaa acggcctgac gtgaatcaag caattttttt
     3001 caggttttgc ccgcttagtg cggtaacaat cctttactca gtaataatat ttcagtgttc
     3061 tttgcgcacg cgctctatat ttatggctaa aaacataatc tctgcgggtg aaattttacg
     3121 ttgatactgc aaaccaataa aaatggcgat ccgttccgca cattgccatg cttgcgggta
     3181 attttgtttt actgcttgtt gtaatgattc atcactatcg ttaattgaag catgttcaag
     3241 aatacgccag gataaaaact tcagatgtgt aaccagtcgc tgataactca agctttcttc
     3301 ctggtaatta aggctgaact gaaattttat taattgcagc atttcgcgca ttaactgcgt
     3361 gacacctgca acatcctcca tatttccgct catttgggca ctgaccagat gcatggcaat
     3421 aaagcccact tcatctttcg gtaactgcac gcccaaccgt ttatcaataa tggttaacgc
     3481 ttcttcccct agctggaact ctttcgggta aagccgctgg atatcccaca gcaacacgtt
     3541 ttgctgaaag cgtttaatcg caaactggca atggtcagtt agcgagatat aaatactgtc
     3601 ctgtaatttt cccaagcgct cctgcgctaa agagataaga cgatcacagg ttgccatcac
     3661 ctcaagagga atatgactta agagttcgct taatcgcccg ttcagttcat gactgctcaa
     3721 ggcatactct ttttctattc cacttgagtt aattctttcg ccagcgcgtt tttgaaagcc
     3781 aattccgcgc cccatgacga ctttttcccg ctgttgatca tcaataacca ccacaacatt
     3841 attgttgaga attttggtga tttgcatgtt catagcaagg acctttttta taaacaaaaa
     3901 aaaaccgact tcaccagtat tctctggtta tgtcaggttt tgcctgcgaa tgcagtaaca
     3961 atccagtcat ttattaatgg tttttataac gaacatccag gttcggaaat taatttaatt
     4021 gcgtgcagag aaaatagcaa tgcgctattg ataaaaatat gaccatgctc gcagttatta
     4081 actttgtgta attttaggaa tttataaagt tatatataac aaatcccaat aattaagtta
     4141 ttgggatttg tctggtgaat tatttgtcgc tatcttttcc cgccagcagt ttatccagct
     4201 catcaccgcc tacatgacgg aaatcctgac ccttcacgta gtagaagata aactcacaaa
     4261 tattctggca gcggtcgccg atacgttcga ttgaacgcgc acagaacagc gcggtaagta
     4321 cgctcggaat agtgcgcgaa tcttccatca tgtaggtcat cagttgacga acaatacctt
     4381 cgtattcctg atcgactttt ttatcttcac gataaatacg taccgcttcg tcaatgtcca
     4441 tccgcgcgaa cgcgtccagc acgtcgtgca gcatctggat ggtatgacgg cccagcgact
     4501 ccagacttac cagcaacggc tgatgctgct gggagaattt ctccagcgca gtacggcaga
     4561 ttttgtccgc cacgtcgcca atacgctcca gctcggcaat ggttttactg atcaccataa
     4621 ccagtcgcag gtcgctcgcc gtcggctgac gtttggcgat aatgcgcacg caggcttcat
     4681 cgatcgccac ttccatcatg ttgacgttct tgtcgccttc gatcacgcgc ttcgccagat
     4741 cgctgtcctg gttatgcatc gcggtgattg catcagaaag ctgctgctcc accatgccgc
     4801 ccatggtcat cacctgcgtg cggatacttt ccagttcggc gttgaactgg ccggaaatat
     4861 gtttattaag attgagactg tccataacgc actcctgaat caaccgtaac gaccggtgat
     4921 gtagtcttct gtttgtttct tcgctggctt ggtgaacaga tcgtccgtgt tgctgaactc
     4981 aatcaattcg cccaggtaca taaatgccgt gtggtcggaa caacgcgcag cctgctgcat
     5041 gttgtgggtg acgatcacca cggtgtaatc ctgcttcagt tcggtgatca gctcttcaat
     5101 acgcccggta gagatggggt cgagcgccga acacggttcg tcgagcagca gcacttccgg
     5161 gcgaatggcg ataccacgcg caatacacag acgctgttgc tgaccaccag agagagagta
     5221 accgctctgg tgcaatttat ctttggtttc gttccacaat gcggctttgg tcaatgccca
     5281 ctgcacgcgc tcgtccatgt cggcacggga gagcttctca aacagacgaa cgccaaaggc
     5341 gatgttgtcg tagatggaca tcgggaacgg cgtcggtttc tggaacacca tgcccacttt
     5401 cgcacgcagc agggcgatat cctgagagtt ggtcaggatg ttgtcgccat caagcagaat
     5461 ttcaccttcc gcacgctgct ccgggtacag ttcaaacatt ttgttgaagg tacgcagcag
     5521 cgtcgattta ccgcagccgg acggcccgat aaacgccgta acctggtttt tggcgatatc
     5581 caggttgatg tttttcaggg catggaattt gccgtagtag aagttcaaat tacgaacctg
     5641 aattttactc ggggcagttt caaccatact cattgcaatc ttttcctcat cgtacctgat
     5701 gcttttcgct aatcaggctt accgtagggc cgggtaaggc gttcacgccg catccggaaa
     5761 agtgttaacc gtgtttattc ttcgcaaaaa caacgcgcgc cagaatgttc agcagcagga
     5821 cgcacagggt aatgatcaat accccggccc aggccagttg ttgccattcc gcaaacgggc
     5881 tcatcgcaaa cttaaagatc gtcaccggca ggttggcgat cggctgcatc atgtccgtgc
     5941 tccagaactg gttggagagc gcggtaaaca gcagcggcgc ggtttcaccg gcaatacggg
     6001 caatcgccag caggataccg gtcataatcc cggacaccga cgctttcagc gtaatcgcgg
     6061 agatcatctt ccacttcggt gtacccagcg cataagccgc ttcacgcagg ctgtccggca
     6121 ccagtttcag catgttctca gtggtgcgga taacgatcgg cacctgcaac agcgccagcg
     6181 caattacgcc cgcccagccg gagaagtgct ccatctgtgc caccacgatg gtgtagacaa
     6241 acagaccaac cacaatcgac ggcgcggaga gcagaatgtc gttaatgaag cgaatcactt
     6301 ctgccagcca ggatttacga ccatattccg ccagataaat ccccgccata atgcccagcg
     6361 gcgtaccgaa taccgtggcc cacaaaatta acagcccgct acccgccagg gcgttcgcca
     6421 gaccaccacc ttccgtattg ggcggcggcg tcatttcagt gaacagcgcc agcgacatac
     6481 cgtcgatacc gcgagtgatg gtggacatta aaatccagat cagccagaac aggccgaagg
     6541 ccatcgtcgc catcgagagc gtcagcgcaa tacggttttt gaggcggcga cgcgcctgca
     6601 ttttgcggcg agattcagcc agcgccgcag tggtttgcat ttcaaccata gccattagcg
     6661 tgccccctca ttcttagcca ggcgcataat cataaacttc gatgcggcga ggacgatgaa
     6721 ggtaatcaca aacaggatca ggcccagttc catcagtgcg gcaacgtgca gaccggattc
     6781 cgcttccgca aattcgttcg ccagcgcaga ggtgatacta ttgcccggca tgtacagcga
     6841 ggcgctgtcg agctggtagg tgttaccgat gataaaggtc accgccatgg tttcaccgag
     6901 cgcgcggccc agccccagca tgatgccgcc gataacacca tttttggtga acggaagaac
     6961 gatacgccag ataacttccc aggtggtgca gccaataccg taggccgact ctttcatcat
     7021 caccggggtt tgttcgaaca catcacgcat taccgccgca atgtacggaa taatcatgat
     7081 ggcgaggatc acgcctgccg cgaggatacc gataccaaat gcggggccag agaacagcgc
     7141 gccaacaatc gggatattcg acatgatatt gccgaccggc tcctgaaagt aaacggcgaa
     7201 cagcggcgca aagataaaca ggccccacat gccgtaaacg atacttggaa tggctgccag
     7261 cagctcaatg gcgataccca gcgggcgttt cagccagcca ggcgcaagct cagtcaggaa
     7321 cagggcgata ccgaaactca ccgggacggc gatcagcagc gcgataaacg aagtcaccaa
     7381 cgtaccgtag atcggcacca gcgccccgta gatatcgttc ggtgcatccc actctttggt
     7441 ccataggaaa gccagaccaa atttctgaat gctcggccag gaggagatga tcagagagac
     7501 aataatgcca cccaacatca atagcacaat cagcgccgcc agttttacca gcacgctgaa
     7561 aattatgtcg ccctttttac ccggtgggtt aaaagcgggc ttggttgcag ccataagtta
     7621 ctcttcagtt aaacgcgttt acaaagtcat tttgtaatgc cggatgcggc gtaaaacacc
     7681 gtacccggcc tggagtttta ttagtacagc ggcttaccgc tactgtcttt aatattggtc
     7741 ttccacgcag cgcgaacctg ttcaactaca ctatccggca ggctggcgta atccaggtcg
     7801 ttcgcctgtt tagccccggt tttgtacgcc cagtcgaaga atttcagcac ctctgtgcct
     7861 tgttctggtt tcttctgatc tttgtggatc agaatgaacg tggtagaggt aataggccat
     7921 gcatcttcgc ctttctggtt ggtcagatcc tgagcgaagg ttttgctcca gtctgcacct
     7981 tttgctgcat tagcgaagtt ttcttcggtc ggactaaccg gtttaccatc agcggagatc
     8041 agtttggtgt acgccaggtt gttctgcttc gcgtaagcat attcaacata accaattgca
     8101 cccggcagac gctgaacgaa cgcggcgata ccgtcgttac ctttaccgcc cagaccgatc
     8161 ggccatttta cggtagagcc agtaccaacg ttgtttttcc actcttcgtt cactttcgcc
     8221 aggtagctgg tgaagacgaa ggaagtcccg gagccatctg cgcggcgtac tacagcaatg
     8281 ttttgtgaag gcagtttcag acccggattc agtttggcga tggcttcatc atcccacttc
     8341 ttgattttgc ccaggtagat gtcgccgagg gttttaccat ccagcaccag ttcgccagac
     8401 ttcagccctg gaatgttaac cgccagcacc acgccgccaa tcacggtcgg gaactggaac
     8461 agaccttcct gagccagttt ttcgtcagac agcggcgcgt cagaggcacc aaaatcaacg
     8521 gtattagcga taatctgttt tacgccaccg gaagaaccga taccctggta gttaacttta
     8581 ttaccggttt ctttctggta agtgtcagcc catttggcat acaccggcgc agggaaggtt
     8641 gcacctgcac ctgtcaggct tgcttctgca aacacagaga aagcactcat cgataaggtc
     8701 gcggcgacaa cagttgcgac ggtggtacgc ataactttca taatgtctcc tgggaggatt
     8761 cataaagcat tgtttgttgg ctacgagaag caaaatagga caaacaggtg acagttatat
     8821 ataaggaata tgacattttt atgacagaaa ggaattacca aataacttac tgattttaaa
     8881 tgtgaagatg aaaattccac atgccatctt tttgtcatga tgcatttaaa agtgataaag
     8941 aaatgggtga gatgacaacg atgtgatgaa caatcgttgt cagacctatt agcgcacaat
     9001 ctcaacagtt atcgtcgctg ttgcagtaaa ttgccccggg gctgggcgag ctccagtaag
     9061 gctcgcagga gcagctgaaa aagttacact acctgatttt tcaccataca ctgttgattt
     9121 atccatgtct gttggcaact caccaccgtt cactttgatt tccttattat ttttgtcgta
     9181 aatttttatc cccacgttag agtttgaagt tacgaccatt gaattgtccg tattgggttc
     9241 ggtagcataa aaagacgtac tgatatcatc ggtattcagt atccccaggt tttcacactc
     9301 aatagtgact gttttagtaa ttttatgtgt tgttaccgca cttccaacaa cagatgaaaa
     9361 atctgcagct ggtatttctt taaaatcaaa acaaactggc tttaaattat ttatttcgca
     9421 actttgcgga gctgtaatat cgccccgaat ataaacatca gccatcggct gtgagtaatc
     9481 caccggggtg tcacggctaa ttgttccata caatgtcact atctttgttt caggaataat
     9541 caattttcct gcaaatgtct tcttgatata aaaatatact atcgctttag ccccactgga
     9601 tatacttaaa ggttcaatgc ggttgcaatt gtactttgta ttaccagacg ggttgttagc
     9661 atgatattca aaaggtgcat tcacataccc aaccccaaga atatctatag caatcccaac
     9721 atcaaggttc ggattaagat cataataatt aatattagag tgaaccccag cataagttaa
     9781 tgcctggttt atttttgacg tataatagat ataggggaat tctcctacgt taccagtatc
     9841 acaatgacag cgtccaagat atgaggatcc attagttaca gtggtgttaa atgtcgttcc
     9901 aaccttattt tcgttcgcgg taataagata tccc
//
LOCUS       DAJTSA010000129         9925 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000129 DAJTSA010000000
VERSION     DAJTSA010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.129"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..336
                     /locus_tag="OO369_003564"
     CDS             <1..336
                     /locus_tag="OO369_003564"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983695.1"
                     /translation="SSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLNA
                     RNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYNI
                     DPLCECFKY"
     gene            524..1639
                     /locus_tag="OO369_003565"
     CDS             524..1639
                     /locus_tag="OO369_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983696.1"
                     /translation="MFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVGPDILAL
                     PVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFD
                     LRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRSVALAKT
                     VAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISVRNKYSP
                     AFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAKDRTISI
                     VLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRVLHVSNK
                     FNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            2318..2536
                     /locus_tag="OO369_003566"
     CDS             2318..2536
                     /locus_tag="OO369_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR5983697.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2754..2996
                     /locus_tag="OO369_003567"
     CDS             2754..2996
                     /locus_tag="OO369_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983698.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3326..4255)
                     /gene="fdhE"
                     /locus_tag="OO369_003568"
     CDS             complement(3326..4255)
                     /gene="fdhE"
                     /locus_tag="OO369_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR5983699.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4252..4887)
                     /gene="fdoI"
                     /locus_tag="OO369_003569"
     CDS             complement(4252..4887)
                     /gene="fdoI"
                     /locus_tag="OO369_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5983700.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4884..5786)
                     /gene="fdoH"
                     /locus_tag="OO369_003570"
     CDS             complement(4884..5786)
                     /gene="fdoH"
                     /locus_tag="OO369_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR5983701.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5799..8849)
                     /gene="fdnG"
                     /locus_tag="OO369_003571"
     CDS             complement(5799..8849)
                     /gene="fdnG"
                     /locus_tag="OO369_003571"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8262..8264),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5983702.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9043..9876
                     /gene="fdhD"
                     /locus_tag="OO369_003572"
     CDS             9043..9876
                     /gene="fdhD"
                     /locus_tag="OO369_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR5983703.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2489 a         2396 c         2542 g         2498 t
ORIGIN      
        1 tcgagtaata atgaatatga cctgtttaaa aatagagaaa cctcactgtg gaaacgcaaa
       61 gtaccagaaa gcgcagcgct ggcaatgacg ggaacagtat ataaattatt tggtaatcgc
      121 ttactcaacg cccgcaatca gttagataaa aatctcctcg aaatggctat gcaaatggtt
      181 tatggcaaag atatggtgac aaagtccggg gatatattga ttgatataca attaaataag
      241 gatggcagtc tccagagtaa gcactacaca cttgatgtag gtgaagttat aaacagttat
      301 aatattgacc ctctttgtga atgcttcaag tattaatcaa acttacttac atgataaaaa
      361 tcctggtgac gttattagcc tgtatggcac tttagataac attccccttg tagttaagga
      421 atgcacgggt aaaaatcatc aaattaatgt taaagccaaa ttgccacaag aacgaagtga
      481 aaaagttgag gatatgtgtg aaagaatgaa aggtggcatc tctatgttta accacaccac
      541 aaaaacagct gataatatag agcataattt gcagctaagt tttctcgtgg gttcacgtcc
      601 gtcgatcacc aataaatact cagcaaaaga tgtaggtcca gatatacttg cattgcccgt
      661 caacgtatat catgcggatt ttaaaattaa aatcaataat gcagtggaga aggcttcatc
      721 aattgtgtta tgtgctgtgc aaaaaaatct ccatgcagca aggaaggttc ttagcgggat
      781 aataggaaat gtgccaaaaa ataataaacc aaaaacaatt atttttgatt tgcgttttct
      841 tagtgcaatt aagcagaaag tttttttggg cagtgaaaaa ccgttgtttg taaagcatac
      901 cgcaatgatg agggaggcat gtaaggaatt accgcagtca gtggaatatg ttcctttagc
      961 ctgtgaagcg catagccatc gcagcgtcgc ccttgccaaa accgtggctg cgcccatcat
     1021 ggcgacaggt gtgggcttgg cattaatgta tgcaacatca tcaggaccgt attatgcgca
     1081 aagtctttct ggttcgccgg atatcgagat gataaaaaat cgcatggctg aattgtttaa
     1141 ccagtcggat atttctgtgc gcaacaaata tagcccggct tttgataaat ggaatgaact
     1201 ggtagataaa ttaaatcacg aaagaaatac agttccgttt gcgtgtttga ccaccatttt
     1261 agcgacggcg ataaatgaaa ctcttgaggg ggatacgaat gttgccgttg tgatggggtg
     1321 taaatctgcg aaagatcgca cgatatccat tgtccttggc aactcgatgt tgcaaacact
     1381 ccttgaaaaa cgcctcgccg atagtggaga aatagaaaat ctttttgatc aacagggata
     1441 ttttagttgc gattcattaa cacccgaaga attaatgatg ttaaaagacc tttttgatat
     1501 tcgagttctt catgtctcta acaaattcaa tgttggattg caaggtaata tcaacactga
     1561 tgttttacaa gattcgtttt ttaaaaatgt cgatttcatt catgaatata actcgtatac
     1621 cgtagggatg ggagtctgac gagtaaaact cattaggatt atcagaattc ggacaatgca
     1681 ttgcaaaaat gacatacaaa gagagatgtg tgcaggtact tagccgcgag gggcatggcg
     1741 aagccataca gaaagtacca gtattcagaa ctccagtgaa agcggcatag tggccgcctg
     1801 ctcaggagtt ttgctatgac ccgtaatcgt gctgaacgtc gccaccctat gatgcgatta
     1861 aaaaaactca tcgtcgcgct gatgattgac gtcaatgcta tcggatgatg ccgggggtgt
     1921 tccggaagga attccttgtt tcccgaactg ttaccaacaa aattataacg atgagcaagt
     1981 aaagaccggt tggtggcagt actgaccggt ctgtgatgaa agattaattt caccttaaat
     2041 cctggtgctg ttaggatgct gacgtttttc ggctacacca ttcagattat gtaaataaat
     2101 atccctgaat ataatatatt catagcaccc ataactcatc ctgggcgata actaaaccat
     2161 ttttgagtaa taaatgaaac aactgcaacg ctcttattat ttctggaaaa cgcctcgcaa
     2221 aaacaaaatg acatgcaaaa atccgcattt ttttaaatgc ttttccggcg taattttcgc
     2281 acatacatta atgagtaata tatgtaaggt ggtggtaatg gcgatgaata cggtttttct
     2341 tcatttatca gaagaggcaa ttaaacggct gaacaagctt cggggctggc gtaaggtttc
     2401 acgatctgca attttacgcg aagcggttga gcaatatctg gagcggcagc aatttccggt
     2461 gcgtaaagca aagggaggca ggcaaaaggg ggaggtagtt ggtgttgatg atcagtgtaa
     2521 ggagcataag gaatgaaaaa aatttttcat tcttgaatat aaaaaacaga tgcccttatt
     2581 ctggtattaa tacaaggctg ttttacttga acttataata actgcaactg ttacatcgta
     2641 tctggaaaac gcctcgcaaa aataaaaaat gatgcgtaaa tgagctttct ttaaagattc
     2701 ggcaagggta cttttctctc catagattca aaaatgatat tggcgagata tttatgaact
     2761 ctctggcagg tatagatatg ggcagaattt tactcgattt atcgaatgag gtgattaagc
     2821 aacttgatga tctcgaggtg cagcgtaatc ttcctcgtgc ggacctatta agggaagcgg
     2881 tagatcaata cctgataaat caatcgcaaa cagcaagaac cagtgttcct ggcatctggc
     2941 aagggtgtga ggaagatggt gtcgaatatc agcgtaagct gcgcgaggaa tggtaatggg
     3001 aaaaaatgat gtcaatcaga ttgctgacaa cgtgcgcgtt gttcatgccg gatgcggcgt
     3061 gaacgcctta tccggcctac aaagtcatgc aaattcaata tattgaagtt tattggtagg
     3121 cctgataagc gcagcgcatc aggcaatcat acgcttgtca tcagtgacat acccataacc
     3181 ttatcgatgt gttggaggga agaacgaggc aaacagacaa ttgcggcatg atcttgaatg
     3241 ggtaacgcgg aatacgagag atggtgtcgt cacaccctat gagctgtaat ccgggcgacc
     3301 tgcgccaccc ggaaaacgtc agagattact ctccttcacc cggaaacagg aacgggttaa
     3361 tggaactacg ggcgtagcct tcttgctcca ttcgcgcgtc cagtaccaga gaggcgaggt
     3421 catctgccac ggcttcaact ttcggttctt tttcctgata aagaatcttc aggtaagtgc
     3481 cgcagtcatc gcagctttcg gctttaatcg cggcctgttc gtcatccagc gaccagtaat
     3541 gcagtttgcc gctctgctcg cagttgctgc attttacgcg cactacgtgc cattcggttt
     3601 cacacaggtt gcagtgcagg taacgcagac cctgagtggt gccaatttgc accatgctgg
     3661 acaccggcat agagccacat accgggcaat attgacgttg ttcgccgtat tcagcgcggg
     3721 ctttgccggg gatcagattg gccatctgcg cccagtagag cgacagcgca gcccagataa
     3781 acggcgcttt atcgctgctg acggacgaga aatcagaggc aaacagcgcg ctggccatat
     3841 cttccagctc ctgagtcgat gccttctcca gattctcaat cactgccagc gccgggccgc
     3901 tcatttcagg tttcagctca gcaattagcg ccatcagcag cttttgccag tgcttatcac
     3961 gcggcagaac gtgaatatcc agcgggggct tgccctgtgc gctggcttct ttaatgcgcg
     4021 cggtcagatc catctccagc ggatggtcgt acagcaccac tttctgggcg tgggcgataa
     4081 gcgcagcaaa gcgcaggtaa tcacccagcg gattattttc tgccagctcg cgcagacgct
     4141 cggcgcggcg gttgtataaa ttcttgagcc gagggaacaa taacggcgga atcatatccg
     4201 ccgtacgttt ctcgctcgaa cccagctcat cttgcgggat tatgcgaata ctcattcagc
     4261 tttcttttcc gttgtcttgc ggacctcacg gtaccagcgc gggtgatgtt tcttcgccca
     4321 ggcgctggta acccatcctt ccaccatggc ggtaatcgtg cctttcaccc aaagggcggc
     4381 gtagatatgc accatgataa ccacaattaa cgccactgcg gcaaatgaat gcagcattaa
     4441 cgcgaatcgg atcaccggga ttgagaaagc aggcgcaaaa taaggacgcc agataatcac
     4501 gccgctcacc agcagcagaa ccaggaaaat aatcgccgcc cagaaaacgc atttctgacc
     4561 gaagttataa cgcccggtgt cacctacttc ctcgttgacg acgatcttac gaatattctt
     4621 cgcccaaaag atatcatccc gattgattag gttttgatgc cagtaacgga aaaacatgat
     4681 gatgaacgag gcaaacataa ccacgccgac aaacgggtgc agaattcgcg ccagctgcgg
     4741 tgtgcccatg atttgcatca accagttgaa ggacgggaac aaaaagccca gcccgctcac
     4801 cgccgccagg atgaagcaga aggcggtgat ccagtggttg atacgttccg gcgcggtgta
     4861 gcgcacgatg gtgtcacgtc gtttcatttg cgctcctcgt ctttctcttc gtgcagatta
     4921 ttctcttcct catccgcacg gttcggaccg acacccacgt agtggaagat actggccgca
     4981 aaggtagccg caaagccaac agccgcgagc ggtttccaga tgcctttcca gaatttcacg
     5041 gtttcgctga tttccgggtt ctccggcaag ccatgataca gatttggctt gtcagcatgg
     5101 tgcagcacgt acatgacgtg tgtaccaccg acgcctgccg gatcgtacag acccgcattg
     5161 tcgtaaccgc gggttttcag ctcagcaacg cgctcgctcg ccagcgtttt catcgactct
     5221 ttcgtgccga agtgaatcgc gcccgttggg caggtcttca cgcaggctgg ttcttgccca
     5281 accaccacgc ggtcaacgca cagcgtacat ttgtagacgc ggttgtcttc cgggttgagg
     5341 cgcggaatgt cgaacggaca gcccgcaatg caataaccgc agccaatgca ctgctcggac
     5401 tggaagtcga cgataccgtt ggcatactga ataattgcac cttccgccgg gcacgctttc
     5461 aggcagcctg gatcggaaca gtgcatacag ccgtctttgc ggatcagcca ttccagtttg
     5521 tcgttctgct ccacttccga gaagcgcatt accgtccacg atttggcgct taaatcattg
     5581 gggttgtcgt acaccccaat gttattgccg acggtatcgc gaatgtcgtt ccactctgaa
     5641 cacgccacct gacaggcttt acagccgata caggtggtaa cgtcgatgag tttcgccact
     5701 tcttcctgga agtcccgcgc ctgaggcgcg ggggtcagac cgttagtcgc ggaacgacga
     5761 atgatatctt gagattgata agccatatgt cgtctccgtt acaccttttc cacattcaca
     5821 aggaaggact taaactccgg cgtctgcgtg ttcgcatcac caacgaatgg cgttaaagta
     5881 ttggcaataa agcctttttt agctacgcct tcatagcccc agtgaatcgg aataccgatg
     5941 gtatcgatat ctttgccgtt cgctttcagc gtgcgaatac gtttggtcac caccgctttg
     6001 gctttgatat agccacggtt ggaggagact ttcacggtat cgccctgggc aatgccaagt
     6061 ttattcgcca gcgactcccc gatttccaca aactgctctg gctgcaaaat cgcgttcaac
     6121 agcgcgtgtt tggtccagta gtggaagtgc tcggtcagac gataggtggt tccgacatac
     6181 gggaacttat cggctttacc caatgcttcg gcgtcgtctt taaagatacg tgcagctggg
     6241 ttcgagataa cgtttggatg cagcgggtta gttcccagcg gcgtttcaaa cggctcgtag
     6301 tgttccggga acggaccttc cgccatctta tcgagggcaa acagacgccc catgccttcc
     6361 tgctgcatga taaacggccc gacgccgcta cccggaggcg ctgcgctgta gtccggaata
     6421 tcccagccgg tccacttagt gccgtcccat ttcagcaact gacgcttcgg atcccacggg
     6481 ttaccctgcg gatctgcgga ggcgcggtta tacagaatgc ggcggttaag cggccatgcc
     6541 catgcccagc ccagcgtgtt accgaggcca gacggatcgg cgttatcacg acgcgccatc
     6601 tggttgcctt ccggcgtcca gctaccggcg aaaatccagc agccacagga ggttgtaccg
     6661 tcatcgcgca attgggcgaa cgagctaagt tgttggcctt tcttgacgat aaccgcaccg
     6721 gttgccggat cggtaatatc ggccagcgcc ttaccgttgc tctccatcgc tacttcttcc
     6781 gacttcggct catgcggaat ggcgtagttc caggtcatgt tcagcacctg atccgggttc
     6841 gcgccaccct gttcggcata catcttgcgc aagcgcagga agataccgga gaggatctca
     6901 ccgtcagtca gcgcaatccc cggggcgtcc gcacctttcc agtgccactg caaccagcga
     6961 cctgagttaa cgattgaacc gttctcttct gcgaagcagg tcgatggcag acggaacact
     7021 tcggtctgga tcttcgacga gtcaacttcg ttcagctcac cgtggttctg ccagaagtta
     7081 gaggtttcag tgttcagtgg gtcgatggtg acgaggaact tcagtttcga cagacaaccg
     7141 atcactttgt ttttgttcgg gaatgaggca acagggttaa agccctggca gatatagcca
     7201 ttgaccttgc cctctttcat catctcgaag tattgcagga cgtcgtagcc tttatcccac
     7261 ttcggcaacc agtcaaagcc ccagctattt tccgccgtcg ctttatcacc aaagaaggcc
     7321 ttcatcatag agacgaagaa tttcgggtag ttgccccagt agtttacctg gccttccagc
     7381 agcggttttg gcgtgttggc ggtaaggtag gtttgcagat cggtctgctt ctcgcttggc
     7441 agcgtcatgt aacctggcag actctgcgac agtagcccca ggtccgtcag cccctgaata
     7501 ttggagtgac cgcgcagggc gttaacgccg ccgccggcca tccccatatt gccgagcagc
     7561 agctggatca tcgccatcgt acgaatgttt tgcgcaccaa cggagtgttg cgtccagccg
     7621 agggcataca ggaacgaggc agttttatcg tgagcactgg tttctgcgat gtattcgcag
     7681 actttcagga acgcgtcttt tggcgtacca cagatgtttt caaccacatc tggcgtgtag
     7741 cgggaaacgt gctgtttcag caagttccac acgcagcgcg gatgttgcag cgtggtatcg
     7801 cgtttggcga agccgttttc gtccagttca taagtccagg aggatttatc gtacttgcgt
     7861 ttttccgcgt cgtagccggt gaacaggcca tcttcaaagc cgtaatcctc acgcacgatc
     7921 aggctggcgt tggtataggc ttcggtgtat tcgtggttga atttttcatt gttcagcagg
     7981 tacagcaata cgcctgacag gaaagcaatg tcagtaccgg aacgaatagg ggcatagtag
     8041 tcagctaccg ccgccgtacg cgtaaagcga ggatcgatca caatcagctt cgcgccgttg
     8101 tgaattttgg cttccatcgc ccagcggaac ccgaccgggt gagcttcagc ggcgttaccg
     8161 cccatcacca cgacgaggtt ggcgttcttg atgtcgaccc agtggttggt catcgcaccg
     8221 cgaccaaatg ttggagcaag acttgctacc gttggtccgt gtcagacacg cgcctggttg
     8281 tcgaccgcga gcatacccag cgcgcgggag aatttttgcg ttaaatagcc ggtttcgtta
     8341 ctcgacgcgg aagcacacag catcccggtg gagagccagc ggttaacagt cacgccttcg
     8401 gcgttttgcg caatgtagtt agcatcgcgg tcttctttca tcagtttggc gatgcgatca
     8461 aacgcctctt cccaactgat ttgctgccat ttatcagaac ctggcgcacg gtattccgga
     8521 aacttcagac ggctttcgga gtggatgaaa tccaccaggc cagcgccttt cggacaaagt
     8581 gcaccgcggt tgaccgggtg atccggatcg ccttcgatat ggaagataga tgctttggcg
     8641 ttttttgctc cgtcaccgag gctgtacatc aacagcccac agcctacgga acaataggtg
     8701 caggtattac gggtttcgcg ggtgcgcagc agtttatact gccgggtttc cgcgagtgct
     8761 acgctgggtg caaaacccag tgccgctgcc gtggtgcctg ccataccgcc agcgcagatc
     8821 ttaaagaact gccttctgct gacctgcatg gattgctcct tgtttcgaca ttgtcacgtc
     8881 ccatttacat tcgcttgctg cgtgtgcagg gagtgggagt tatttttctt tgcggaaggg
     8941 gccgcaaagg tccagaattg gctcaatttc cctccatcta ggaagggttt gtaacagaat
     9001 accataatgt tggtgtgtat gttcttatct ggttaagaga aagtgaaaaa aacacagcga
     9061 aaagaaatcg aaaatgtgac aaatatcaca ggtgttcgtc aaattgagtt atggcggcgt
     9121 gatgatttac aacacccacg gttagatgag gtcgcggaag aagttcccgt tgcgctggtc
     9181 tacaacggca tttcgcatgt ggtgatgatg gcgtcgccca aagaccttga gtactttgcg
     9241 ctcggttttt cgctttccga ggggataata gaaagtccgc gcgatatctt cggcatggat
     9301 gtcgttcctt cctgtaatgg tcttgaagta caaattgagc tttccagccg ccgctttatg
     9361 gggttgaagg agcgccgccg ggcgctggcg ggacgtacgg gctgtggcgt atgcggcgtg
     9421 gagcaactta atgacatcgg aaaaccggtg cagccgctac cgttcaccca gacgtttgat
     9481 ctcaacaaac tggatgatgc attacgtcat ctcaacgatt ttcagccagt ggggcgactg
     9541 actggttgta ctcacgccgc tgcctggatg ttgccatctg gcgaactggt cggcgggcat
     9601 gaagacgtgg gtcgccatgt ggcgctggac aaactgttag gccgccggtc acaagaaggg
     9661 gaaagctggc agcaaggtgc ggtactggtt tccagtcgtg ccagttatga aatggtgcaa
     9721 aagtcggcga tgtgcggcgt agagattttg tttgcggtgt ctgccgcgac cacgcttgct
     9781 gtagaagtgg ctgagcgctg taatctgaca ctggtaggtt tttgtaaacc gggtcgggca
     9841 acggtttata cccatccgca gcgtttgagc aattaattaa aataagtcct gcgatatata
     9901 ttaaattgca ggacttattc atttc
//
LOCUS       DAJTSA010000130         9906 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000130 DAJTSA010000000
VERSION     DAJTSA010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.130"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(160..900)
                     /gene="pflA"
                     /locus_tag="OO369_003573"
     CDS             complement(160..900)
                     /gene="pflA"
                     /locus_tag="OO369_003573"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR5983704.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="OO369_003574"
     CDS             complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="OO369_003574"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5983705.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3429..4286)
                     /gene="focA"
                     /locus_tag="OO369_003575"
     CDS             complement(3429..4286)
                     /gene="focA"
                     /locus_tag="OO369_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR5983706.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4692..6452)
                     /locus_tag="OO369_003576"
     CDS             complement(4692..6452)
                     /locus_tag="OO369_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR5983707.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6582..7208
                     /locus_tag="OO369_003577"
                     /pseudo
     CDS             6582..7208
                     /locus_tag="OO369_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7407..8495
                     /gene="serC"
                     /locus_tag="OO369_003578"
     CDS             7407..8495
                     /gene="serC"
                     /locus_tag="OO369_003578"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR5983708.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8566..9849
                     /gene="aroA"
                     /locus_tag="OO369_003579"
     CDS             8566..9849
                     /gene="aroA"
                     /locus_tag="OO369_003579"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR5983709.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2521 a         2367 c         2483 g         2535 t
ORIGIN      
        1 ttcgatgggg aagaacttca cgcgagctat tatcagtcgt tattatctca ggtacgggat
       61 atggtagatg cactataaga tgtgttaaaa acgctgtagc agaatgaagc gcggaataaa
      121 aaagcggcaa ctcaataaag ttgccgcttt acgaggaaat tagaacatta ccttatgacc
      181 gtactgctca agaatgcctt tcacgcgttc catggtctct ttcttcggtg gtttaacacc
      241 atcgagtttg tattcttcac ccatcgccac ccatttgtgt ttgcccagtt cgtggtaggg
      301 gagaagttcg attttctcaa cgttacccat atcacgggta aattcaccga ggcgatgtgc
      361 cgaatcgtca tcgtcagacc agcctgggac aacaacgtag cggatccaaa ccttcacatt
      421 tttgttcgcc agatatttag cgaactccag cgtgcggtgg ttggaaactc caaccagatt
      481 ttggtggatc tcgtcgttca tctgtttgag atcgagcatt accaggtcgg ttacttccag
      541 cagttcatca atcaccggat cgtaacgacg aacaaaaccg ttggtatcca gacaggtatg
      601 aatgccttct tttttacagg cgcggaacca gtcacgaaca aactcagctt gcaggattgc
      661 ttcaccgccg gatgcggtaa cgccgccgcc ggaagcgttc ataaagtggc gataggtcac
      721 cacttccttc atcaaatctt caacggtaac ttctttaccg ccatgcgtgt cccaggtgtc
      781 gcggttatga caatacaggc agcgcatcag gcagccctgg aaaaaggtga taaagcgaat
      841 acctgggccg tctacggttc cacaggattc aaaggagtga atgcgaccaa taactgacat
      901 tgcggtgttt ctccagatgt ggcccatctg aggccgtgtt ggtgcgcagc tcgaaggcta
      961 cgtcgagtct gttttggcag tcaccttaaa gtatagatag ctgacaaaaa aggctctcgt
     1021 gctaaaaaag gccccacttt cgtggagcct ttattgtacg ctttttactg tacgatttca
     1081 gtcaaatcta attacataga ttgagtgaag gtacgagtaa taacgtcctg ctgctgttct
     1141 ttagtcagcg agttgaaacg tactgcgtag ccagatacac ggatggtcag ctgcggatat
     1201 ttttccgggt tttccatcgc gtcgagcagc atttcacggt tcatcacgtt aacgttcagg
     1261 tgctgaccac cttcgatgga tgcttcgtgg tggaagtaac catccatcag accagccagg
     1321 ttggtcttac gaacttcgtc gtctttaccc agtgcgttcg gaacgataga gaaggtgtag
     1381 gagataccat ctttagcgta agcaaacggc agtttagcaa cggaagtcag agacgctaca
     1441 gcacctttct ggtcacgacc gtgcatcggg ttagcacccg gtccgaacgg cgcgccagca
     1501 cgacgaccgt ctggggtgtt accagttttc ttaccataca caacgttaga agtgatggtc
     1561 agaacagact gagtcgggat agcgtcacgg taggtgtgca gtttctgaat tttcttcatg
     1621 aaacgttcta ccaggtcaac agccaggtca tctacacgcg gatcattgtt accaaactgc
     1681 gggtattcgc cttcgatttc gaagtcgata gccagaccgt cttcgtcacg aatcggttta
     1741 actttcgcat atttgattgc agacagggag tcagcagcaa cggacagacc agcgatacca
     1801 cacgccatgg tgcggataac gtcacggtcg tgcagcgcca tcagagaggc ttcgtagctg
     1861 tacttgtcgt gcatgtagtg gatgatgttc agtgcagtga tgtactgttt agccagccag
     1921 tccatgaagt gatccatgcg ctccatcact tcatcgtagc tcaggagatc gcctttgatc
     1981 ggttcagact tcggaccaac ctgcattttc agtttttcgt caacgccgcc gttgattgcg
     2041 tacagcatgg ttttcgccag gtttgcacgc gcaccgaaga actgcatttg tttaccaacg
     2101 atcatcgggc ttacgcagca agcgatagcg tagtcatcgt tgttgaagtc cggacgcatc
     2161 aggtcatcgt tctcatactg cagagaagag gtgtcgatgg acactttagc ggcgaatttc
     2221 ttgaagttca gcggcagttt ttcagaccac agaatggtca tgttcggttc cggagacggc
     2281 cccatagtgt acagggtgtt caggaaacgg aagctgtttt tggtaaccag ggtacgacca
     2341 tcgaggccca taccaccgat agattcggtt gcccagattg ggtcgccaga gaacagttca
     2401 tcgtattccg gagtacgcag gaagcgaacc atacgcagtt tcatgaccag gtggtcaacc
     2461 atttcctgcg cttcttgttc ggtgatcttg ccagctttca ggtcacgttc gatgtacaca
     2521 tccaggaagg tggaggtacg accgaaggac attgcagcac cgttctgaga cttaacagca
     2581 gccaggtagc cgaagtaagt ccactggata gcttcctgag cgttggtagc cggaccagag
     2641 atgtcgtagc cgtatttcgc agccatttct ttcatctgac ccagagcgcg gtgctgttca
     2701 gcgatttctt cgcgcagacg gatagtctgt tccaggttta cgccgttttc cagatcagcc
     2761 tgtagagagg tgaactgagc gtatttgtct ttcatcaggt agtcgatacc gtacagcgca
     2821 acgcgacggt agtcaccgat gatacggcca cggccataag catctggcag accggtcaga
     2881 acaccggatt tacggcaacg caggatgtcc ggagtgtaaa cgtcgaacac gccctggttg
     2941 tgagttttac ggtattcagt gaagattttt ttgatcatcg ggtccagttc gcggttgtac
     3001 gctttgcagg aaccttcgat cattttgata ccaccgaacg ggataagagc acgtttcagc
     3061 ggagcttcag tctgcagacc aacaactttt tccaacgctt tattgatgta gccagcgtcg
     3121 tgagaggtga tggtggaagc aacagcggtg tcaaaatcaa ctggcgcgtg agtgcggttt
     3181 tccagtttaa caccttccat tactttgtcc cacagggtgg tggtcgcttc agtagcgcca
     3241 gccaggaagg actcgtcacc ctcgtacgga gtgtagtttt tctgaatgaa gtcacggacg
     3301 tttacttcat tctgccagtc acctttggta aaaccttccc aggctgtggc taacttttca
     3361 ttaagctcgg acatgtaaca cctaccttct taagtggatt ttttatttac tgcgtacttc
     3421 gacaaccatt aatggtgatc gttttcacgc aggtaaatga cccagtatgt caacccaacc
     3481 aacaaaccac cgccgataat attaccgatc gtaaccggga tcaggttatc agtgatgaag
     3541 ttcatcacgg tcaggtgaga aaaattttcc ggtgcagaac cgacagcggt ccagaattcc
     3601 ggagatgcga agtcgcggat tacaataccc atcgggatca taaacatgtt tgcgatactg
     3661 tgctcaaaac cgctggcaac aaacatcgcg accggcagca ccataatgaa cgctttgtcc
     3721 atcaggctgc ggccagaata actcatccat actgccagac ataccatcag gtttgccagg
     3781 ataccaagac agacggcctc aataaaagta tggtgcactt tgtggtcggc ggtttgtagg
     3841 acgtttagtc cccattgacc atttgcggtc atatactcgc cggaaagcca cattaaaagt
     3901 acaaacagca gtgcgccgac caggttgcca aaatagacat ttagccagtt tttagccaac
     3961 tgacaccagg tgatgcgccc actcgcctta gcaacaacaa tcaacactgt ggaagtaaag
     4021 agatcggctc cgcagacaac acaaagaatc agccccagag agaagcaaat gccgccaacc
     4081 agttttgcca tgccgaaggg cattgtgcct gtgccagtgg ttgctgtgat atagaagacg
     4141 aatgcgattg agatgaaaac accggcggta atcgccagat agaaagtctt aagcggatgt
     4201 ttcgttgctt tatagacacc cgcctcttcg gccactttgg ccattgcagc aggaagtaaa
     4261 agatcaaaag ggttgtcagc tttcacacta actctctctt tattaagtcg gcgacgagat
     4321 actaacaaag cattatagat gagaaattga tatagatcat atctcgcctg gcttataggc
     4381 ccgtaactcg catggttttt atgcaaatac ggagtaacta tttgattatc caaataaaaa
     4441 taaattttaa aaattaacaa atgagttgaa ttttttccgc atcctccgct aaaacagtta
     4501 attaaaaggg agcatcaggc gaataaagta acaatatcga tcgtatttat taaatacaaa
     4561 ttaccgatat ttaaccttat aattacaatt attttattaa tgcaaatata tgtaaagcgg
     4621 ggcattaaaa aaacgccccg taatataact cagactaatc attaagccta cattgcgtag
     4681 gctatttgat tttattttgc ccagaatgct gctttggcgc gctgcagctt ttcgtaggcc
     4741 ttcaacaacg actgatgtgc agcaaacgcg tgcagatcgc tatctaccgg ttgcaggccg
     4801 taaaacgccg cttcgccgct cattgccgca ctggcggctt ctacggcatc tgcgccgtac
     4861 atgcgaacaa acgcattcag atattgcagc ggctggcgat cttcttcctg tgccagtaat
     4921 aacaacgttt gcaggcagcg ataatagttg gcgcgttccg gactaaatac tgatgagtta
     4981 aactccatcg tccattcggt ccagaccaga gcctgttcca gatcgccacc agccagcgcc
     5041 agcatggctt ttaattcacc gatacgcagg gtgtaccaac cgttatccga cccggtcgcc
     5101 agacccaaca gctcacgcac gcgggtaaag tcatcaaaac cttcttcatc cagttgctcg
     5161 atgaggttca ggtaatcttc tttttcccac tcgctgcctg gtagcgaaag aatcgtttca
     5221 cgtaaatggc tgcccatact gttattcgcg agccacagat cttcagccgg ataaatatcg
     5281 gacatgccag gcacgataat acggcaagca taaacgccca gatgctcgta atcggcaata
     5341 taaacttctt tatcttcttt gttgaagata gccatcagcg tagcaaactc ttcttccgtg
     5401 gtgccggaga aattccagtc cacaaacgga taatcggcat cctgcttgaa caggtcccag
     5461 gagattaaac cgctggaatc gataaagtgc gtttcgaggt tggtatgttc agcgacttct
     5521 tcatcatcga aggttggcgg agtaaacaca tccaaatctt tcaggccacg accttgcagc
     5581 agctcggtca cggtacgttc cagtgctacg ccaaaatcag gatgcgcacc gaaagaggca
     5641 aagcaggtgc cgtttgcagg attaaacagt accacgcaaa tcaccggata ctggccgcca
     5701 agggaaccat catatgcgaa gatcgggaaa ccttctgttt ccagtgtttc gatcgcttca
     5761 accaccgctg ggtaacgcgc cagcacgtct gccgggattt ccggcaagct gatgctttca
     5821 gcaataatgc ggtttttcac gtagcgttcg aaaacttcgg acaacccctg aacgcgtgct
     5881 tcgttgcggg tattgccagc ggacataccg ttagaaacgt acaggttacc aatgatattc
     5941 atcggaatat aaacggtctg attgtcggac tggcgcgtaa acggcagacc gcaaatacca
     6001 cgatcttcgt taccggattg taggtcaatc agcatactgc cggtcaattc attctccgga
     6061 tcgtaaaatg cgcgcagacg gtcatcgagc agcccttctg gcacatcgtc attttcgctc
     6121 agtgggaacc atttttcgtt gggataatgc acaaatggac cgttggcgat ggtttcgccc
     6181 agccagaaat cagcaaaaaa gtagttggtt gagagacgct cgaaatattc accgagtgca
     6241 gaagccagcg ccgctttctt ggttgcgcct ttaccgttgg taaaacacag tgcgcactct
     6301 ttgtcgcgaa tatgtacaga ccagacgtta ggcacgggat tcagccagga ggcctcttca
     6361 atctgaaagc cgaggtctga aagtttttgc tggaagcgag cgatggaatc ttccagagcg
     6421 gcatctttgc cggggataaa tgtttgcgtc atgaaaatca ctttagtcgt acggaaagcg
     6481 cgcaataata cgggttttat ctcaaaggcg ctatcaccgc cgccatgccg atgaactgtt
     6541 gactatgctt ttagcggata aaccacgtta agagcataaa aatgaaggcg ttcgatctcc
     6601 accgtatggc atttgataaa gtgccttttg atttccttgg cgaagttgca ctacgtagtc
     6661 tttatacctt tgtactggtt ttttgttcct caaaatgacc ggaagacgcg gtgtgcgaca
     6721 gatgtcgctg tttgaagtgt taatcattct cacgctgggt tcagcggcgg gagatgtggc
     6781 gttttatgat gatgtgccga tggtgccggt acttatcgtc tttatcactc tggcgttgtt
     6841 ataccgcctg gtaatgtgat ggcgagctgg cctggtcgaa actcaataac tccaacatga
     6901 cggaatttga gttctttatg gagctacgat tgcgtggcgt ggagcagctg gggcaggtac
     6961 gtctggcgat tctcgaaacc aacgggcaaa tcagtgtcta tttctttgaa gatgacaagg
     7021 tgaaaccggg tttacttatt ttacccagtg attgtacgca gcgttacaaa gtggtgccgg
     7081 agtcggcgga ctatgcctgc attcgttgta gtgaaatcat tcatatgaac gcgggggaaa
     7141 aacaattatg tccgcgctgt gcaaatccag aatggacgaa ggcaagtcgg gcaaaacggg
     7201 tgacctgaca gtaaaaacat cggctttttg ctaataatcc gagagattct tttgtgtgat
     7261 gcaagccaca tttttgccct caacggtttt actcattgcg atgtgtgtca ctgaatgata
     7321 aaaccgatag ccacaggaat aatgtattac ctgtggtcgc aatcgattga ccgcgggtta
     7381 atagcaacgc aacgtggtga ggggaaatgg ctcaaatctt caattttagt tctggtccgg
     7441 caatgctacc ggcagaggtg cttaaacagg ctcaacagga actgcgcgac tggaacggtc
     7501 ttggtacgtc ggtgatggaa gtgagtcacc gtggcaaaga gttcattcag gttgcagagg
     7561 aagccgagaa ggattttcgc gatcttctta atgtcccctc caactacaag gtattattct
     7621 gccatggcgg tggtcgcggt cagtttgctg cggtaccgct gaatattctc ggtgataaaa
     7681 ccaccgcaga ttatgttgat gccggttact gggcggcaag tgccattaaa gaagcgaaaa
     7741 aatactgcac gcctaatgtc tttgacgcca aagtgactgt tgatggtctg cgcgcggtta
     7801 agccaatgcg tgaatggcaa ctctctgata atgcggctta tatgcattac tgcccgaatg
     7861 aaaccatcga cggtatcgcc atcgacgaga cgccagactt cggcaaagat gtggtggtcg
     7921 ccgccgactt ctcttcaacc attctttccc gtccgattga cgtcagccgt tatggtgtaa
     7981 tttacgctgg cgcgcagaaa aatatcggcc cggctggcct gacaatcgtc atcgttcgtg
     8041 aagatttgct gggcaaagcg aatatcgcgt gtccgtcgat tctggattat tccatcctca
     8101 acgataacgg ctccatgttt aacacgccgc cgacatttgc ctggtatcta tctggtctgg
     8161 tctttaaatg gctgaaagcg aacggcggtg tagctgaaat ggataaaatc aatcagcaaa
     8221 aagcagaact gctatatggg gtgattgata acagcgattt ctaccgcaat gacgtggcga
     8281 aagctaaccg ttcgcggatg aacgtgccgt tccagttggc ggacagtgcg cttgacaaat
     8341 tgttccttga agagtctttt gctgcaggcc ttcatgcact gaaaggacac cgtgtggtcg
     8401 gcggaatgcg cgcgtctatt tataatgcca tgccgctgga aggcgttaaa gcgctgacag
     8461 acttcatggt tgagttcgaa cgccgtcacg gttaatgccg aaattttgct taatccccac
     8521 agccagcctg tggggttttt atttctgttg tagagagttg agttcatgga atccctgacg
     8581 ttacaaccca tcgctcgtgt cgatggcact attaatctgc ccggttccaa gagcgtttct
     8641 aaccgcgctt tattgcttgc ggcattagca cacggcaaaa cagtattaac caatctgctg
     8701 gatagcgacg acgtgcgcca tatgctgaat gcattaacag cgttagggtt aagctatacg
     8761 ctttcagccg atcgtacgcg ttgcgaaatt atcggtaacg gcggtccatt acacgcagaa
     8821 ggtgccctgg agttgttcct cggtaacgcc ggaacggcaa tgcgtccgct ggcggcagct
     8881 ctttgtctgg atagcaatga tattgtgctg accggtgagc cgcgtatgaa agagcgcccg
     8941 attggtcatc tggtggatgc gctgcgcctg ggcggggcga agatcactta cctggaacaa
     9001 gaaaattatc cgccgttgcg tttacagggc ggctttactg gcggcaacgt tgacgttgat
     9061 ggctccgttt ccagccaatt cctcaccgca ctgttaatga ctgcgcctct tgcgccggaa
     9121 gatacggtga ttcgtattaa aggcgatctg gtttctaaac cttatatcga catcacactc
     9181 aatctgatga agacgtttgg tgttgaaatt gaaaatcagc actatcaaca atttgtcgta
     9241 aaaggcgggc agtcttatca gtctccgggt acttatttgg tcgaaggcga tgcatcttcg
     9301 gcttcttact tcctggcagc agcagcaatc aaaggcggca ctgtaaaagt gaccggtatt
     9361 ggacgtaaca gtatgcaggg tgatattcgc tttgctgatg tgctggaaaa aatgggcgcg
     9421 accatttgct ggggcgatga ttatatttcc tgcacgcgtg gtgaactgaa cgctattgat
     9481 atggatatga accatattcc tgatgcggcg atgaccattg ccacggcggc gttatttgca
     9541 aaaggcacca ccacgctgcg caatatctat aactggcgtg ttaaagagac cgatcgcctg
     9601 tttgcgatgg caacagaact gcgtaaagtc ggcgcggaag tggaagaggg gcacgattac
     9661 attcgtatca ctcctccgga aaaactgaac tttgccgaga tcgcgacata caatgatcac
     9721 cggatggcga tgtgtttctc gctggtggcg ttgtcagata caccagtgac gattcttgat
     9781 cccaaatgca cggccaaaac atttccggat tatttcgagc agctggcgcg gattagccag
     9841 gcagcctgaa tgaacaacgg gcaataaata gccaaatctt tctttatcaa aacgtcggca
     9901 cattgt
//
LOCUS       DAJTSA010000131         9796 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000131 DAJTSA010000000
VERSION     DAJTSA010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9796
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.131"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            383..1501
                     /locus_tag="OO369_003580"
     CDS             383..1501
                     /locus_tag="OO369_003580"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5983710.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1567..1815
                     /locus_tag="OO369_003581"
     CDS             1567..1815
                     /locus_tag="OO369_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR5983711.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1880..2248
                     /locus_tag="OO369_003582"
     CDS             1880..2248
                     /locus_tag="OO369_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5983712.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2342..2995
                     /gene="nfsB"
                     /locus_tag="OO369_003583"
     CDS             2342..2995
                     /gene="nfsB"
                     /locus_tag="OO369_003583"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR5983713.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3103..4350
                     /gene="ybdG"
                     /locus_tag="OO369_003584"
     CDS             3103..4350
                     /gene="ybdG"
                     /locus_tag="OO369_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR5983714.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="OO369_003585"
     CDS             complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="OO369_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR5983715.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="OO369_003586"
     CDS             complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="OO369_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR5983716.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="OO369_003587"
                     /pseudo
     CDS             complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="OO369_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2397 a         2718 c         2376 g         2305 t
ORIGIN      
        1 gttgatagaa atatctcctg gggcttcatc tttccagacc tcagagtaaa cctgaaatcg
       61 gaaagcttca ggcacccgca gttatcttac ccttcctcaa caaaatagct gtttatttat
      121 acagcaaatc ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt
      181 tttttctgca tgcctcaatc gtcaaaattc ttcgccacca gccagctctg ccataacttg
      241 ccacattgaa aaagtagcca aagtgcttca ggcaccatca gttcaccatc accaaaaaaa
      301 agaaacattc cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa
      361 aacgtcaact taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac
      421 cctcggtatt gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga
      481 ctcttcaatg ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga
      541 tatcaccgaa agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc
      601 cgggcagttt tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga
      661 aatttgcggc ggtggcacgc ccccgtttca gaaatggcag cgtcaggagg tatgcgacaa
      721 cgaacgctat caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt
      781 tggtcagcat gtccatgttg gctgtgccag tggcgatgac gccatttatt tgctgcacgg
      841 cttgtcacgg tttgtgccgc actttattgc cctttccgcc gcgtcgccat atatgcaggg
      901 aacggatacg cgttttgccc cctcacgacc gaatattttt tccgcctttc ctgataatgg
      961 cccgatgccg tgggtcagta actggcaaca atttgaagcc ctgtttcgct gtctgagtta
     1021 caccacgatg atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt
     1081 tggcacggtg gaggttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat
     1141 ggcgggatta attcaggcca ccgcccactg gttactgacg gaacgcccgt tcaaacatca
     1201 ggagaaagat tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atggtctgga
     1261 aggcgtcatt accgatccgc acactggcga tcgtcgaccg ctaacggaag ataccttgcg
     1321 attgctggaa aaaatcgccc cttcagcaca taaaattggc gcatcgagcg caattgaagc
     1381 actgcatcgc caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc
     1441 cgatggcggc tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccggtgacta
     1501 agccgggaat tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac
     1561 aatgttatga aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct
     1621 ttcctctctt gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg
     1681 gtgaccatgt tccatctgat ggatcttagc cagctttaca ctttattgtt ttgtctgtgg
     1741 tttttagtgc tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc
     1801 tcgctggcgg attaaacgtc gattatcgcc gaacttcgcg gcatactttg cttattctcc
     1861 ttcgcctaaa ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctagc
     1921 tcttgagtta ccctttgtcg agctttgctg gccttttggc ccggagttcg atgtttttaa
     1981 aattggcggc aagattttta tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct
     2041 gaagtccgat ccacaaaaat ccctgttaaa tcagcaaata tacccaagca ttaagccagg
     2101 gtatcacatg aataaaaagc actggatttc ggtgtattcc ggcgaggaaa tctccgaagc
     2161 gttacttcgc gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg
     2221 cgatcaaaaa agagtgcgtc caggctaaag cgggaatcta tagcgcattt ttctcgctta
     2281 ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa ttacttacac atggagtctt
     2341 tatggatatc atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa
     2401 aaaacttacc ccggaacagg ccgagcagat caaaacgcta ctgcaataca gcccatccag
     2461 caccaactcc cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt
     2521 tgccaaatcc gctgccggta attatgtgtt caacaaacgt aaaatacttg atgcctcgca
     2581 cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga
     2641 ccaggaagat gccgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg
     2701 tcgcaagttc ttcgcctata tgcaccgtaa agatctgcat gatgatgcag agtggatggc
     2761 aaaacaggtt tatctcaacg tcggtaactt cctgctcggc gtggcggcgc tgggtctgga
     2821 cgcggtgccc atcgaaggtt ttgacgccgc aatcctcgat gcagaatttg gtctgaaaga
     2881 gaaaggctac accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa
     2941 cgctacgctg ccgaaatctc gtctgccgca aaacaccacc ttaaccgaag tgtaattctc
     3001 tcttgccggg catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt
     3061 ttcagccgtc atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc
     3121 caggttgaag atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc
     3181 ggtattattt ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg
     3241 accttcgaaa aacgtgccat cgccagttca cggctttggt tgcaaatcat tacccagaat
     3301 aaactcttcc accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta
     3361 ttctggctgc aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg
     3421 atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg
     3481 cagaaattcc cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg
     3541 atcggcgcaa tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg
     3601 attctaatca gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg
     3661 attcttggtc tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac
     3721 tggctggaaa tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc
     3781 gtcaaagtgc gtaactggga caataccatt accactattc ccacctggtc tctggtttct
     3841 gactccttta aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt
     3901 atcagtattg atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat
     3961 aaagcgcatt tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat
     4021 cgccagcaag gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc
     4081 tttcgtgcct atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc
     4141 ttaatggtac gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt
     4201 actaacaccg tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata
     4261 tttgcgattg tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt
     4321 cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga
     4381 gagcggacat cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa
     4441 atgccacaaa caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat
     4501 ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga
     4561 acggatagag cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gccgcgcgaa
     4621 aacgcagatg cgccaggcag atcataatcc agttcaacag caacgttgct accaccagcg
     4681 ccatcagcag accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag
     4741 tgatcgctcc ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca
     4801 aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa
     4861 ccccgctgtt atacaccgac agcgatgcca ctagaatgac gaagttcagc gcagaagcta
     4921 ccacattgct gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt
     4981 ccacccacgg atagagtgcc agtaaaacca ccagtgaacc gatataaaac agcaggatgc
     5041 gatacaccac ctgatttacc gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg
     5101 ctgtaatccc aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa
     5161 tcagcccatt ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg
     5221 ctttctcgcc gccgtaacca gaaaacagca gccacaggcc aaagccgatc ataccgatga
     5281 tcgccagcac tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca
     5341 ccaggttaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat
     5401 ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca
     5461 cgaacattac ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat
     5521 aggcaaagtg ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc
     5581 gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg
     5641 ccatctgaat cgccgggcca atgccaagaa acagaccagt accaattgcg ccacccagcg
     5701 caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg
     5761 cagtatcttc cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt
     5821 tgaggggcac cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa
     5881 cgaggtatcc tgctttaatt ccgtacccga tgtcggtgca gccacatcag cttatacgcc
     5941 gccgggataa taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggtgcg
     6001 gcaatccggc tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg
     6061 gcgataatca ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc gccgtgatac
     6121 aacgcctcat ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggctcggcc
     6181 tctatggcgt gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggca
     6241 agggcaataa agccggtacc cgtcgccaca gaaagatgaa agcccatcca ccacagtaac
     6301 cagatgccgc ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc
     6361 cgacggaatg ccagatacaa cagcacgaag atgatcatca gtgtcatcgg caccatgagt
     6421 ttcagcttat ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg
     6481 cccggtttta actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc
     6541 atgtcacgat cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc
     6601 agcatcgacg gtccggtaga gactttaacg tcggccacgt ctgccagggt gatttgctgc
     6661 ttcatcggcg tcaggatcgg caactggcgc agtgcctgcg gactatcgcg ccagctttgc
     6721 ggataacgca gattaattgg ataacgggca atcccttcca ccgtttcgcc aaccatcgcc
     6781 ccgcccaccg cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg
     6841 gctttttcac ggttaatctc aacgttgata tagcgcccac cttccagccg ctcagcaagc
     6901 gcagaagcta cgcctggcac cgttcgcgcc acttcttcaa tttgctcagc catcgcgtcg
     6961 atatccgcca gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag
     7021 agcatatcga tacggttacg aattggcggc acccacagat tcgccagccc cggcagacgc
     7081 acggtgttat ccagttcctc aatgattttg tccattgtca tgcctggccg ccactgatcc
     7141 tgtggcttaa gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt
     7201 tccgctttcc cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg
     7261 tcggtttttt gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc
     7321 atatacaaca agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc
     7381 ggccagagaa ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc
     7441 agtactttca gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cggattactg
     7501 ctttccggcg gaattttgcc acggatccag taacccatca ggatagggat cactacgatc
     7561 gccagcagcg ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga
     7621 cgcccttcct gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga
     7681 ctgataaaca gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt
     7741 ttattatcca gcgtggcgtc aggatgctgg tgctgccact cttccagccg tttatgcgca
     7801 ttctcgatca tgacgatagc ggcatcgacc atcgccccga cggcaatcgc aatgccaccc
     7861 agcgacataa tattggcatt cagcccctgg aagggcatga caataaaagc aatacacaac
     7921 cccagcggca acgaaataat cgccaccagc gccgagcgca catgccagag aaacggcgcg
     7981 cagactaccg ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcc
     8041 cggtcaatga gctggctgcg gtcgtatgtt gtaactatct ccacgccttc cggcagacta
     8101 cttttcagcg tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg
     8161 gatcgcagga tcaccacccc gcccgccact tcgccttcgc cgtttagttc ggcaatgccc
     8221 cggcgcatct tcgggccaat ctggaccttc gcaacatcgc gcagataaac gggcacgcca
     8281 ttttcactgg cttttaaaac gatgtgatta aagtcgtcga gcgtttgtag atagccgctg
     8341 gcgcgcacca tatattctgc ttccgccagt tcgattgacg aaccgcccgc ttcctggttt
     8401 gaagcatcca gcgcgctttt tacttcggcg agactgatgc catactgcgc caggcgctga
     8461 ggatcgataa ccacctgata ctctttcacc acaccgccta ccgacgccac ttccgcaacg
     8521 tcaggaatgg ttttcagctc atatttgaga aaccagtcct gcaatgagcg taaatcggca
     8581 agatcgtgct taccgctgcg atccaccagt gcatattcat agatccagcc aacacccgtg
     8641 gcatctggtc ccagctcggc gctgactccc gcaggcagct taccctgtac ctggttgagg
     8701 tactccagca cccgcgaacg cgcccagtac ggatcggtgc catcttcgaa aatgacatac
     8761 acataagagt cgccaaactg cgagaaacca cgcacagtct tcgcgccagg caccgacaac
     8821 atggtggtgg ttagcggata agtcacctga ttttcaacga tttgcggtgc ctggccggga
     8881 tagctggttt taataatcac ctgcacatcg gagagatccg gcaacgcatc cactggcgta
     8941 ttaatgatgg tccaggtgcc ccagatgctc agaaacaacg cgcccatcag caccaaaaaa
     9001 cggttcgcca ccgagcgacg aataatccat tcaatcattg gttattccct caatgcgcat
     9061 gggtagcact ttcagagcgc atccgctcca gtgcgccaga aatattggct tcggaatcaa
     9121 tcaggaacag gccgccggaa accacctttt caccttccgc cagaccagag cgtaatgcgg
     9181 tgacgccttg cgatgcctgg aaaacagcaa cgcgtttcgg tacaaagcgc ccgtcggcat
     9241 caacggtaat cacccgctgt tcgttgccgg tatcaatcag cgcctgtgac ggaatgagca
     9301 gcatcggttc gctggcggtg ttgagttgca accaggcgtt catgcccggt tttagcgcct
     9361 cgtcggcgtt gtcgacttcc agacgcagct gcagcgtgcg ggtcgcggca tccacgccag
     9421 gtagcagcgt ccatttgcgg atggtgagtg ttttatccgg tcgcgccgga acggtgaggg
     9481 taaactgcga ggcatctttc accagccagg cgatggactc cgggatcgca gcagtgaccc
     9541 acaccgggtc cataccctga attcgcgatt acgcgcaatc cccctgagaa ccgccatgaa
     9601 caagggtttc atgatttgtg attttagttt ggtacgcaat ttggtacaca acactatttt
     9661 cacttcagcg ggtagtcatc aaactcacca taacgagcat cgttgatgat gtatgtgatc
     9721 accacaaata tcacatctgg actagttccg tcttcatgcg caggtagact ggccccctga
     9781 atctccagac aaccag
//
LOCUS       DAJTSA010000132         9772 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000132 DAJTSA010000000
VERSION     DAJTSA010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.132"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            390..2021
                     /gene="oppA"
                     /locus_tag="OO369_003588"
     CDS             390..2021
                     /gene="oppA"
                     /locus_tag="OO369_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR5983717.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2107..3027
                     /gene="oppB"
                     /locus_tag="OO369_003589"
     CDS             2107..3027
                     /gene="oppB"
                     /locus_tag="OO369_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR5983718.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3042..3950
                     /gene="oppC"
                     /locus_tag="OO369_003590"
     CDS             3042..3950
                     /gene="oppC"
                     /locus_tag="OO369_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR5983719.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3962..4975
                     /gene="oppD"
                     /locus_tag="OO369_003591"
     CDS             3962..4975
                     /gene="oppD"
                     /locus_tag="OO369_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR5983720.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4972..5976
                     /gene="oppF"
                     /locus_tag="OO369_003592"
     CDS             4972..5976
                     /gene="oppF"
                     /locus_tag="OO369_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR5983721.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6029..6358)
                     /locus_tag="OO369_003593"
     CDS             complement(6029..6358)
                     /locus_tag="OO369_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR5983722.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6393..7853)
                     /gene="cls"
                     /locus_tag="OO369_003594"
     CDS             complement(6393..7853)
                     /gene="cls"
                     /locus_tag="OO369_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR5983723.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7996..8169
                     /locus_tag="OO369_003595"
     CDS             7996..8169
                     /locus_tag="OO369_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR5983724.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8224..9477)
                     /gene="kch"
                     /locus_tag="OO369_003596"
     CDS             complement(8224..9477)
                     /gene="kch"
                     /locus_tag="OO369_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR5983725.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2563 a         2286 c         2432 g         2491 t
ORIGIN      
        1 aaagatgttt gttaaggcat ggatgcaagc tatagattct gatacggtca ataaaagaga
       61 atcgcttaac aattttgcaa aatgtattgg cgagtaagaa ccgcatttgg tactttccgg
      121 gcaaccgcca gacgattctt tattggtaat gagaataatt aacaattaaa gagcgtcgcg
      181 aaagaataat gtgtctcgac aggggagaca cagtacgaat cgacataagg tgatcgtctg
      241 aatcaccaga ataaataaag tcggtgatag taatacgtaa cgataaagta acctgacagc
      301 agaaagtctc cgagcttgtg cagggtccca atccgggatt acacatgctg gttaatacca
      361 gtaattataa tgagggagtc caaaaaacaa tgaccaacat caccaagaga agtttagtag
      421 cagctggcgt tctggctgcg ctaatggcag ggaatgtcgc gctggcagct gatgtacccg
      481 caggcgtcac actggcggaa aaacaaacac tggtacgtaa caatggttca gaagttcagt
      541 cattagatcc gcacaaaatt gaaggtgttc cggagtctaa tatcagccga gacctgtttg
      601 aaggcttact ggtcagcgat cttgacggtc atccagcacc tggcgtcgct gaatcctggg
      661 ataataaaga cgcgaaagtc tggaccttcc atttgcgtaa agatgcgaaa tggtctgatg
      721 gcacgccagt cacagcacaa gactttgtgt atagctggca acgttctgtt gatccgaaca
      781 ctgcttctcc gtatgccagt tatctgcaat atgggcatat cgccggtatt gatgaaattc
      841 ttgaagggaa aaaaccgatt accgatctcg gcgtgaaagc tattgatgat cacacattag
      901 aagtcacctt aagtgaaccc gttccgtact tctataaatt acttgttcac ccatcaactt
      961 caccggtgcc aaaagccgct atcgagaaat tcggcgaaaa atggacccag cctggtaata
     1021 tcgtcaccaa cggtgcctat accttaaaag attgggtcgt aaacgaacga atcgttcttg
     1081 aacgcagccc gacctactgg aacaacgcga aaaccgttat taaccaggta acctatttgc
     1141 ctattgcttc tgaagttacc gatgtcaacc gctaccgtag tggtgaaatc gacatgacgt
     1201 ataacaacat gccgatcgaa ttgttccaga agctgaaaaa agagatcccg gacgaagttc
     1261 acgttgatcc atacctgtgc acttactatt acgaaattaa caaccagaaa ccgccattca
     1321 acgatgtgcg tgtgcgtacc gcactgaaac taggtatgga ccgcgatatc attgttaata
     1381 aagtgaaagc gcagggcgac atgcccgcct atggttatac tccaccgtat actgatggcg
     1441 caaaattgac tcagccagaa tggtttggct ggagccagga aaaacgtaac gaagaagcga
     1501 aaaaactgct ggctgaagcg ggttataccg cagacaaacc gttgaccatc aacctgcggt
     1561 ataacacctc cgatctgcat aaaaagctgg cgattgctgc ctcttcattg tggaagaaaa
     1621 acattggtgt aaacgtcaaa ctggttaacc aggagtggaa aacgttcctc gacacccgtc
     1681 accagggcac ttttgatgtg gcccgtgcag gctggtgtgc agactacaac gaaccaactt
     1741 ccttcctgaa caccatgctg tcgaacagct cgatgaatac cgcgcattat aagagcccgg
     1801 cctttgacag cattatggcg gaaacgctga aagtgactga cgaggcgcag cgcacagctc
     1861 tgtacactaa agcagaacaa cagctggata aggattcggc cattgttcct gtttattact
     1921 acgtgaatgc gcgtctggtg aaaccgtggg ttggtggcta taccggcaaa gatccgctgg
     1981 ataataccta tacccggaat atgtacattg tgaagcacta atggcaatac gtggggcagg
     2041 agtgtcctgc tccacggtgt ctgattttta tcgcattaca gaaggcacag gccagaaggt
     2101 agggcaatgt taaaatttat tctacgtcgt tgtctggaag cgattccgac gctatttatt
     2161 cttattacta tttcgttctt tatgatgcgc ctcgcaccgg gaagcccttt taccggcgaa
     2221 cgtactttac cgccagaagt gatggccaat atcgaagcga aatatcatct taatgatccg
     2281 atcatgacac agtatttcag ctacctgaaa caactggcgc atggcgattt cggtccatcg
     2341 tttaaatata aagattattc ggtcaatgac ctggtggcat ccagttttcc cgtttctgcc
     2401 aaactgggag ccgccgcatt tttccttgcg gtaatactgg gtgttagtgc tggcgttatt
     2461 gccgcattaa aacaaaacac caaatgggac tataccgtga tggggctggc aatgaccggg
     2521 gttgttatcc ccagttttgt ggttgcgcca ttattagtca tgatatttgc gatcattttg
     2581 cattggctgc cgggcggtgg ctggaatggt ggggcgctta aattcatgat attgccgatg
     2641 gtggcgttgt cactcgccta tatcgccagt attgcgcgta ttacccgtgg ctctatgatt
     2701 gaagtattac actccaactt tattcgtact gcccgggcga aagggttacc tatgcggcgg
     2761 atcattttac gccatgcatt aaaacctgct ctgttacccg tgctctccta tatgggcccc
     2821 gcatttgtcg gcattattac cggttctatg gtcatcgaaa ccatttatgg tttgccgggg
     2881 attgggcaac tgttcgttaa tggcgcattg aaccgtgact attccttagt gttaagcctg
     2941 accatcctgg ttggtgcgtt aaccattttg tttaatgcca ttgtcgatgt gctatatgcg
     3001 gttatcgacc cgaaaatccg ttactgatac tggagctcgc gatgatgtta agtaagaaaa
     3061 acagcgagac gctggaaaat ttcagtgaaa agctggaggt cgaagggcgc agcttgtggc
     3121 aggacgcacg tcgacgtttt atgcataacc gtgcggcggt tgccagtctg atagtgctgg
     3181 tgctgatcgc gttatttgta atcctggcac cgatgctttc ccagtttgcc tatgacgata
     3241 ctgactgggc gatgatgtcc agcgccccgg atatggagtc cggtcactac tttggtactg
     3301 actcatccgg tcgcgacctg cttgtgcgcg ttgcgattgg cgggcgtatc tcactcatgg
     3361 tcggtgttgc tgcggcactg gtggcagtgg tcgtggggac actttatggt tcgctttccg
     3421 gttatctggg cggtaaagtg gattcggtaa tgatgcgtct gctggaaatc ctcaactcct
     3481 tcccattcat gttcttcgtc attttgctgg tgaccttttt cggccaaaac atcctgctga
     3541 ttttcgtggc gattggcatg gtttcctggc tggatatggc tcgtattgtg cgtgggcaaa
     3601 ccctgagtct gaagcgcaaa gagtttattg aggcggcaca agttggcggt gtatcgacgc
     3661 cgggcattgt tattcgccac attgtgccga acgtactcgg tgtggtggtg gtctacgcat
     3721 cgctactggt gcccagcatg atcctctttg aatctttcct tagcttcctg gggttgggta
     3781 cgcaagagcc gttaagcagc tggggggcat tgctgagtga tggcgcgaac tcgatggaag
     3841 tctctccatg gttactgttg ttcccagcgg gattccttgt ggtgacactg ttttgtttca
     3901 actttatcgg cgatggcttg cgtgatgccc tcgacccgaa agatcgttaa ggagtgcagc
     3961 catgagcgta attgaaactg caactgtgcc gctcgcacaa caacaggctg acgcactgct
     4021 gaacgtgaaa gatttgcgtg tcacctttag taccccggac ggcgacgtca cggcggtcaa
     4081 tgatttaaat ttttccctac gtgccggaga aacgctgggc attgtaggtg agtctggttc
     4141 gggtaaatcg caaactgcat ttgcgttgat gggcctgttg gctgccaacg gacgcattgg
     4201 cggatcggca accttcaatg ggcgtgaaat cctcaatttg ccagagcgtg aactcaataa
     4261 actgcgcgct gaacaaatct caatgatttt tcaggaccca atgacttcgt tgaatcccta
     4321 tatgcgcgtc ggtgagcagt tgatggaagt gctgatgctg cataagaaca tgagcaaagc
     4381 tgaggcgttt gaagagtcgg tgcggatgct cgatgcggta aaaatgccgg aagcacgtaa
     4441 acgcatgaaa atgtacccgc acgaattttc tggcggcatg cgtcagcgag tcatgattgc
     4501 gatggcattg ctatgtcgac ctaagttgct gattgcggat gaacccacta cagcgctgga
     4561 cgtcaccgta caggcgcaga tcatgacgct attgaatgaa ctgaagcggg aatttaatac
     4621 tgccatcatt atgatcaccc acgatcttgg cgtggtggcg gggatctgtg acaaagtgct
     4681 ggtaatgtac gccgggcgca cgatggaata tggcaatgcg cgcgatgtct tttatcaacc
     4741 cgttcatcct tattctatcg gtttgctcaa cgcggtgccg cgtcttgatg cggaaggtga
     4801 aacaatgttg accatcccgg gtaatccgcc aaacttgctg cgattaccga aaggttgccc
     4861 gttccagcca cgttgtccac atgcgatgga aatttgtagt agcgcaccgc cgctggaaga
     4921 gtttacgcct ggccgtctgc gtgcttgctt taaaccggtg gaggaactgt tatgaatgct
     4981 gtaactgaag gaagaaaagt cctccttgaa atcgccgatc ttaaagtgca ctttgaaatc
     5041 aaagatggca aacagtggtt ctggcaaccg ccgaaaacgc tcaaagccgt cgatggtgtc
     5101 actcttcgcc tgtatgaagg ggaaacatta ggtgtggtag gggaatcggg atgcggtaag
     5161 tccacctttg ctcgcgccat catcggtttg gtcaaggcga ccgacggtca tgttgcctgg
     5221 ttaggtaaag agttgctggg catgaagccc gatgaatggc gtgccgttcg cagtgatatt
     5281 cagatgattt tccaggatcc gttggcatcg ctaaacccgc gtatgaccat cggcgagatc
     5341 atcgctgaac cactgcgtac ttatcatccg aaaatgtcac gccaggaagt tcgcgagcgc
     5401 gtgaaggcga tgatgctgaa agtcgggtta ttgcctaacc tgattaaccg ctatccgcat
     5461 gagttctccg gtgggcagtg ccagcgtatc gggattgctc gtgctcttat tcttgaaccg
     5521 aagctgatta tctgcgatga gccggtttcg gcgctggacg tgtcaattca ggcgcaggtg
     5581 gttaacctgc tccagcagct gcaacgtgag atgggattgt cattaatttt tattgctcat
     5641 gacctggccg tggtaaaaca catttccgat cgtgtgttgg tgatgtatct cggccatgca
     5701 gtagaactgg ggacctatga tgaggtctac cacaatccac tacatcctta caccagggca
     5761 ttgatgtcgg cagtccccat acctgatccg gatctggaga agaacaaaac catccagtta
     5821 ctggaagggg aattaccgtc gccgatcaac ccgccttccg gttgtgtttt ccgtacccgt
     5881 tgcccgattg ccggtccgga gtgcgccaaa acacgtcctg ttctggaggg gagtttcaga
     5941 cacgccgttt cttgcctgaa agtcgatccg ctttaaaaac aataagggct gacagttgtc
     6001 agcccttttt cacgctaaaa gcgattattt attcccgcca gatgatatgg caaagtttgt
     6061 gatctttttc gcggcataac agaatgcggg cgaaaacatc gctgatttca ccatcttcac
     6121 tgtccgccag accaatcacc acttcggcaa aaaagtccgg gttcaaatcg aaatcaacat
     6181 gttcctgcca gtcttccgcc ggatcgaata actccgcgcc gccgcgctct tcaaactgaa
     6241 gattgaacag cagaacatca gctggatcga gattgtcggc agccagttcg agaaaaatat
     6301 cgtaggcctg ctcaagcgtt tcatcttcag tcaggcgatt gtttagatcc atatccatag
     6361 tcactacctg tttaacctct gttggcgacg ttttacagca acgggctgaa gaagtaaaac
     6421 agtcgctcgg cgacacgttg ccataatgga cgttttagcc ataaacgggc atcgagcaga
     6481 cgtgaacgcg aaatataatc gtcctgaacg gcggcaaggt ctgcaccaaa acctttatcg
     6541 tcgattgcca aggtaatctc gaaattcagc cacagactac gcatatcaag gttaacggtg
     6601 ccaaccagac ttagttcgcc atcgaccagc acgctcttgg tatgcagtaa cccgccttca
     6661 aactgataaa ttttaacccc agcagcaagc agttccgtaa agaatgcgcg actggcccag
     6721 ccgaccagca tcgagtcatt ttttcgcgga aggataatac tgacatccac cccgcgctgc
     6781 gccgccgtgc aaatcgcatg aagtaaatca tcgcttggca caaagtaggg cgtggtcatg
     6841 atcaaatatt catgcgccga ataagccgca gtcaataatg cctggtgaat gagatcttcc
     6901 ggaaagccgg ggccagaagc aattgtgtga atggtgtgac cgctggcctg ttcaaacggc
     6961 ataatattga catctggtgg tggcggcaga atacgttttc cggtttcaat ctcccagtcg
     7021 caggaataaa taatccccat cgcggtggcg atggggcctt ccatacgcgc catcagatca
     7081 atccattgcc ctacgcccgc atcttgtttg aagtagcgag gatcgaccat attcatgctg
     7141 ccggtgtacg cgatgtaatt atcgatcatg atcatcttgc gatgttggcg caggtccata
     7201 cggcgtaaaa acacacgcat cagattgacc tttaaggctt cgaccacttc aataccggca
     7261 ttacgcatta gctcgggcca cgggctgcgg aaaaaagcca cactcccggc ggagtcgagc
     7321 atcaatcggc aatgaatacc acgccgtgca gccgccatta atgattcagc cacctggtcc
     7381 gccatgccgc cgggctgcca gatataaaac accatctcaa tattatggcg cgcgagctgg
     7441 atgtcgcgga ttaacgcctg catcacatca tctgactcgg tcatcagctg tagctgattc
     7501 cctttgaccc cagcgatccc ctgacgacgc tcgcaaagct tgaataatgg cgcagcgaca
     7561 ctgctatttt cttcggcgaa gatatgctta caggctttaa ggtcgttaag ccattttgcg
     7621 gtggaaggcc acatcgctct ggcgcgctca gcgcggcgtt tgcctaaatg gagctcgcca
     7681 acggcaagat aggcaataat tccgactaac ggcagaatgt aaataatcaa cagccaggcc
     7741 atcgcggagg gaactgcgcg tcgtttcatt agaatgcgta aagttacgcc tgcaatgagc
     7801 aaccagtatc ccagaatggc caaccaactc accaacgtat aaacggttgt catagattag
     7861 aaatcctttt gaaagcgcat tgttatgagt ttacgcatat ggattcatct ggcaaataaa
     7921 aacgcgggaa aagcactggt cagcgtcggg ggcgggggta taatgaccat tctgttattg
     7981 catagagtag ttaacatgaa gcggagtaga acggaagtgg ggcgctggcg catgcagcgt
     8041 caggctagcc gacgtaaatc gcgttggctt gaggggcaat cgcgccgaaa tatgcgtatc
     8101 cacagcatca ggaagtgcat tctaaacaaa cagcgtaact cgttattgtt tgcgatctac
     8161 aatatctaaa tgtactgggc accattgcgg tgcccaataa gtttactctt ccgtctttaa
     8221 actctatttt tgcgccgatt ctttactgtc agctttcgtt tcttgttcat cgttatcgct
     8281 gaaaatacca tgacctgagt tcaacaacat tgaaacgagc atatcattat taatctcttc
     8341 accatttaaa actcgcgcca gaatttcgct gccaaacagt tgcggtgaga gaatgatatc
     8401 cggatgtacc atcttaatct tattcaggtt tttgctatca ctgacggcga gaactgtttt
     8461 gacatcactg ctcatatctt ttgccgagag tacaacaaac gcgttatctg catcgttatc
     8521 actcagcgcc agaatggccc ggcaacgatc gattcccgct ttctttaata ctgaactgtc
     8581 attactgtca ccggggataa catcagcgtt atcgcctaag cgttgctcaa gttgcttgat
     8641 atcatcttca ggcaagttgc tgataaccgt tacgttttgt ccgcgttgat tcagttgcag
     8701 gatcgtattg atggcgagaa tcgaatgtcc gcaaacaata aaatgatctt tacgatgcat
     8761 tgtatgattg tttcctttta aaagtttgtt gaatcccccg cggataagcg gaccaaaaat
     8821 tgaggtcata gatgtggcaa aaacggtaat gccggaaata atgaccgaaa tagtgaacaa
     8881 tcgtgctgat tcagaaacag ggacaatatc tccatagccg acggttgaca tggtttctat
     8941 cgaaaaatag aacgcggtca tcaaactttc tattcgcgga ttaaaacctt cgcttaaata
     9001 aagcgcaccg taggttgagt aaaacagtaa tgtcgtgaaa ctaataaatg caaaaattgt
     9061 cccggctgcg gcgctactgt gggagaagtc tttgcgcagt atcagcaaaa acaccagcgt
     9121 aaaaatgcaa aatccaatac taaatttcag ccagggataa aaatgcaggg tgtaaattag
     9181 cgctatcaac aacaaaatga tactgattgc ccatgcgagc tttgcccgga acagtaagcc
     9241 aatggagttc aggacaagaa agacgccgag cataaatagt ggggcgtttg ccagcaagga
     9301 gagatccaac tctgaaaatg ctttgatatg gaaaatatca agcaaattga ccgacatacc
     9361 atagattgtt ttaaaaataa gcaatccatt taaaaagacg gccagcgcga gaatgtcgtg
     9421 ccgtagggtc acccataaat ttgttgctgt ttgtttgaat gtagcccagt gactcactga
     9481 tacctctgta accacaattt tgtttaatta tcttagcgtg cacattttct gaacatacat
     9541 gcagcgcgtg aatgtgttaa acaatagtag atgactttat ccgcagtaat caaaggtttt
     9601 gagtgagcaa ctctgcgtca ggaaagggct tttttcctga acatgctcac atcatataaa
     9661 gaaatacaga taaagttatt atctgcttgt ggtagtgaat gcactgaccg gctataagga
     9721 aaggccaaac aagaacacgg ttgcaaaaac cgtgccctta aatattggat ct
//
LOCUS       DAJTSA010000133         9681 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000133 DAJTSA010000000
VERSION     DAJTSA010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.133"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(26..214)
                     /locus_tag="OO369_003597"
     CDS             complement(26..214)
                     /locus_tag="OO369_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5983726.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMVMTPTY"
     gene            complement(433..1410)
                     /gene="epmA"
                     /locus_tag="OO369_003598"
     CDS             complement(433..1410)
                     /gene="epmA"
                     /locus_tag="OO369_003598"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR5983727.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1735..3543
                     /gene="frdA"
                     /locus_tag="OO369_003599"
     CDS             1735..3543
                     /gene="frdA"
                     /locus_tag="OO369_003599"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR5983728.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3536..4270
                     /gene="frdB"
                     /locus_tag="OO369_003600"
     CDS             3536..4270
                     /gene="frdB"
                     /locus_tag="OO369_003600"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5983729.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4281..4676
                     /gene="frdC"
                     /locus_tag="OO369_003601"
     CDS             4281..4676
                     /gene="frdC"
                     /locus_tag="OO369_003601"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5983730.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4687..5046
                     /gene="frdD"
                     /locus_tag="OO369_003602"
     CDS             4687..5046
                     /gene="frdD"
                     /locus_tag="OO369_003602"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5983731.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5109..6242
                     /locus_tag="OO369_003603"
     CDS             5109..6242
                     /locus_tag="OO369_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5983732.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6331..6864
                     /gene="blc"
                     /locus_tag="OO369_003604"
     CDS             6331..6864
                     /gene="blc"
                     /locus_tag="OO369_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5983733.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6861..7178)
                     /gene="sugE"
                     /locus_tag="OO369_003605"
     CDS             complement(6861..7178)
                     /gene="sugE"
                     /locus_tag="OO369_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5983734.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="OO369_003606"
     CDS             complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="OO369_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5983735.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="OO369_003607"
     CDS             complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="OO369_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5983736.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7788..8354)
                     /gene="efp"
                     /locus_tag="OO369_003608"
     CDS             complement(7788..8354)
                     /gene="efp"
                     /locus_tag="OO369_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5983737.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8396..9424
                     /gene="epmB"
                     /locus_tag="OO369_003609"
     CDS             8396..9424
                     /gene="epmB"
                     /locus_tag="OO369_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR5983738.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2465 a         2480 c         2466 g         2270 t
ORIGIN      
        1 ggcattatct gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg
       61 gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt
      121 aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc
      181 ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg
      241 tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat
      301 acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa
      361 aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt
      421 aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc
      481 gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat
      541 accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc
      601 cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc
      661 atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg
      721 atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa
      781 ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa
      841 cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc
      901 ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg
      961 ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac
     1021 ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc
     1081 agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg
     1141 cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag
     1201 attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat
     1261 atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc
     1321 aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc
     1381 gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac
     1441 gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg
     1501 cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat
     1561 aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca
     1621 gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca
     1681 ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa
     1741 acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt
     1801 gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg
     1861 cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc
     1921 ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc
     1981 gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc
     2041 ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc
     2101 gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag
     2161 acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg
     2221 gttgatgatg gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg
     2281 cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac
     2341 aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt
     2401 ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc
     2461 ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt
     2521 tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat
     2581 atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc
     2641 aacaccatct ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag
     2701 aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc
     2761 gatccggtta aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc
     2821 gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc
     2881 tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc
     2941 ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac
     3001 gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac
     3061 caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa
     3121 ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag
     3181 ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac
     3241 ctgctctaca ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc
     3301 gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag
     3361 cgtgacgacg tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact
     3421 cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt
     3481 ggcgaagcgg atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc
     3541 tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc
     3601 gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg
     3661 ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat
     3721 ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct
     3781 gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga
     3841 tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat
     3901 cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa
     3961 gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt
     4021 tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga
     4081 agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt
     4141 atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc
     4201 tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa
     4261 accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt
     4321 ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg
     4381 ttccggctgt gtggttcagc attgaactga ttttcgcgct gtttgccctg aaaaatggcc
     4441 cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc
     4501 tgatcactct gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag
     4561 cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct
     4621 gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag
     4681 cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc
     4741 ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt
     4801 ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc
     4861 gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt
     4921 ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa
     4981 tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca
     5041 atctaacgca tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac
     5101 cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat
     5161 ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc
     5221 aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta
     5281 cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt
     5341 taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg
     5401 aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga
     5461 atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc
     5521 cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat
     5581 gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg
     5641 ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac
     5701 tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg
     5761 gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg
     5821 gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta cccccccttg
     5881 atatcaatga gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa
     5941 ccggcgatat gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca
     6001 gcatcattaa cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta
     6061 agcccccaac tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat
     6121 ttggtagcta tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca
     6181 aaaactatcc caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt
     6241 aaaatttcat cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac
     6301 acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg
     6361 acagctgcat ttctggtcgt tgcctgtagt tttcctacgc cgccgcgtgg cgtgaccgta
     6421 gtgaataatt tcgacgccaa acgttatctc ggtacctggt atgagattgc ccgttttgat
     6481 caccgttttg aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc
     6541 ggcctgaatg tcattaataa aggctataac cctgacagag gaatgtggca gcagagtgaa
     6601 gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt
     6661 cctttctatg gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt
     6721 tgcggcccgg accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa
     6781 gtgaaacagg agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt
     6841 tgggtacagc agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg
     6901 attaacgcca gactcgccag acgcatcggg ttagcggact caccgagcag cacaatgccg
     6961 gtgatggccg cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt
     7021 aacgatttca tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacggtaata
     7081 acactcggcg tcaaacggct aaagccgtgg gtatatttca ggccaacggc ccataccact
     7141 tccagcagac cagcaataac taagataatc caggacatat caggctccgg aaatttgggg
     7201 ccgtccccgg tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat
     7261 attttgccag gtggcagggg aatttcaact attaccttta tgacgatcgc agaaaaaaca
     7321 aacggcacga cactgctgtc gtaccgttat tgcttattgc tgcgctttcg ttgctgcgcc
     7381 agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaggc
     7441 agttaatact gttgaaagca ccagaacaga aaagatcgct gcaattgtct tcttcaccat
     7501 aacgtcttcc ttttaggtaa tgttatttat gtttgcctat agcaaattaa taatagacaa
     7561 catcgccaaa aatgatggaa atctgatgat ttttcggaag agaagaaaaa ttagctggca
     7621 gcgcgagaga tggagttacc gagatgtttg atgtcttcgc cgaaaccgca agcggtgtta
     7681 cagcccgtga gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt catcatctct
     7741 gacctgaata agtgatggtg cagcctgcag gccgcaccac aaccgcatta cttcacgcga
     7801 gagacgtatt caccagagcg ggtatccact ttgatgactt cgccgatttg tacgaacagc
     7861 ggaactttaa ccacagcgcc agtagacagg gtagccggtt tgccgccagt acctgcggta
     7921 tcacctttca ggcccggatc ggtatcaacg atttccagtt caacgaagtt cggcggagta
     7981 acggagatcg gctgaccatt ccacagagtt acgatacact ctgcctgatc cagcagccat
     8041 ttagcattgt cgccaattgc ttttgcatca gcagacagct gctcgaaagt ttcgttgttc
     8101 atgaagtgcc agaactcacc gtcgttgtac aggtaagtca ggttcatatc gacaacatca
     8161 gcgccttcag cggaatcagt agatttgaag gttttttcta cgcgagtacc ggtcagcaga
     8221 cgacgcagtt taacgcgagc aaatgcctgg cctttacccg gttttacgaa ttcactcgct
     8281 tcaaccgcgt aaggttcgcc gtctaacatg attttaagac cagcacgaaa atcgttgcta
     8341 tagtacgttg ccataaggcc ctctgaaatt tgttaattgg tagctaagcc acaaaatggc
     8401 gcatattgta accctaaata ccccatccag agaagattgg ttaacgcaac ttgccgatgt
     8461 tgtgaccgat cctgatgaac ttctgcgtct tttgaatata gacgcggacg aaaaactgtt
     8521 ggccggacgc agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg
     8581 catggagaaa ggcaatccgg acgatcctct tttgcgtcag gtacttacct cgcaagatga
     8641 gtttgtcgtc gcgtccggat tctccaccga cccactggaa gaacagcaca gcgtagtgcc
     8701 tggtttgttg cataaatacc acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt
     8761 aaattgccgc tattgcttcc gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg
     8821 taactggcag actgcacttg agtatgttgc tgcgcatccg gaactggacg agatgatttt
     8881 ctccggcggc gatccgctga tggcgaaaga gcacgagctg gactggttgc tcacacaact
     8941 ggaagccatc ccgcatatca aacgtctgcg gattcacagc cgtctgccga ttgtgatccc
     9001 ggcacgtatc accgaggcgc tggttgaacg ctttgcccgt tctacgctgc aaatcttgct
     9061 ggtgaatcac atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg cgatggctaa
     9121 attgcgccgt gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg gtgtgaacga
     9181 taacgcacaa acgttggcaa acctgagtaa tgcgttgttc gatgccggcg tgatgccgta
     9241 ttacctgcat gtgctagata aagtacaggg cgcggcgcat tttatggtga gtgatgacga
     9301 agcacggcag attatgcgtg agttgctgac attggtgtcg ggatatctgg tgccgaaact
     9361 ggcgcgagaa attggcggcg aacccagcaa aacgccgctg gatctccagc tacgccagca
     9421 gtaactgatt aaacagggaa gttaaacatt aaaattgcct gatgcgctac gcttatcagg
     9481 cctacatcgt gcattgaaat tgaatggatt tgcaatgaac tgtaggccgg ataaggcgtt
     9541 tacgccgcat ccggcaagag caacaaacgc aagtacagca accactgaga aatttaatct
     9601 ttgcacgttg cataaccttt aattattcat atgcattgct gttgttactt tttatatagc
     9661 taacaccaat aattaacctt t
//
LOCUS       DAJTSA010000134         9595 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000134 DAJTSA010000000
VERSION     DAJTSA010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.134"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(7..327)
                     /gene="psiF"
                     /locus_tag="OO369_003610"
     CDS             complement(7..327)
                     /gene="psiF"
                     /locus_tag="OO369_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR5983739.1"
                     /translation="MQITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(446..1861)
                     /gene="phoA"
                     /locus_tag="OO369_003611"
     CDS             complement(446..1861)
                     /gene="phoA"
                     /locus_tag="OO369_003611"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR5983740.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1962..2222)
                     /gene="iraP"
                     /locus_tag="OO369_003612"
     CDS             complement(1962..2222)
                     /gene="iraP"
                     /locus_tag="OO369_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR5983741.1"
                     /translation="MKNLIAELLFKFAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2404..2607)
                     /locus_tag="OO369_003613"
     CDS             complement(2404..2607)
                     /locus_tag="OO369_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983742.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2685..3779
                     /gene="ddlA"
                     /locus_tag="OO369_003614"
     CDS             2685..3779
                     /gene="ddlA"
                     /locus_tag="OO369_003614"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5983743.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3803..4015)
                     /locus_tag="OO369_003615"
     CDS             complement(3803..4015)
                     /locus_tag="OO369_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR5983744.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4275..4583
                     /locus_tag="OO369_003616"
     CDS             4275..4583
                     /locus_tag="OO369_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR5983745.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4642..5736)
                     /gene="yaiW"
                     /locus_tag="OO369_003617"
     CDS             complement(4642..5736)
                     /gene="yaiW"
                     /locus_tag="OO369_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR5983746.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5749..6969)
                     /gene="sbmA"
                     /locus_tag="OO369_003618"
     CDS             complement(5749..6969)
                     /gene="sbmA"
                     /locus_tag="OO369_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR5983747.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7321..8478
                     /gene="ampH"
                     /locus_tag="OO369_003619"
     CDS             7321..8478
                     /gene="ampH"
                     /locus_tag="OO369_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR5983748.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDPCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8479..8982)
                     /locus_tag="OO369_003620"
     CDS             complement(8479..8982)
                     /locus_tag="OO369_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5983749.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9070..>9595)
                     /locus_tag="OO369_003621"
     CDS             complement(9070..>9595)
                     /locus_tag="OO369_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5983750.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2381 a         2448 c         2285 g         2481 t
ORIGIN      
        1 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
       61 cagagcttgt tgcgttgctt gattattaca ttcacgcatc ttttgctgct gtggcgtcaa
      121 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
      181 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
      241 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
      301 caaggtaacc agtaatgtta tttgcatagc accatccctc ttcatgtttt aaccatgagc
      361 gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag
      421 cgaaaattca ctgccgggcg cagttttatt tcagccccag agcggctttc atggtgtaga
      481 agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa
      541 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca
      601 ccatcactgc gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg
      661 gcgcaacaac ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct
      721 cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa
      781 tttgcccaca agggttcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca
      841 ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca
      901 gggttggtac actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga
      961 tattgccgtg gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa
     1021 acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat
     1081 cgttcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
     1141 caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac
     1201 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
     1261 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca
     1321 gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
     1381 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
     1441 cgccgttata ggttttgaca tcggttgacc aggcggttgc tgatgcagcc gagtcggtga
     1501 cgtagtccgg tttgccagtt tttttattca gcgcatagtg agtgtattgc ccggtaagcg
     1561 gtaaggcatc gataccttta aaaaagccgc ccgcaccttc ggcataatta cgtgcggcag
     1621 taatttccga atcccccatc ccatcgccaa tcagcaaaat aatatttttt gcaggtttat
     1681 cgctaagaga atcacgcaga gcggcggtct gatcacccgt taagcggcga gcaccgccgg
     1741 gtgcagtaat atcgccctga gcagcccggt tttccagaac aggcatttct ggtgtccggg
     1801 cttttgtcac aggggtaaac agtaacggta agagtgccag tgcaatagtg ctttgtttca
     1861 ctttattttc tccatgtaca aatacattaa aaaataaaaa caaagcgact ataagtctcg
     1921 gccgtgacaa ctttatgaca gctgttgaaa agattaactt tttactgaca aggatgcttc
     1981 aataacttct ttacgtaatc gcgcagcagc tctgtatcgt cgtcaggaat gctggcatcg
     2041 ggctttacct cgtacagcgc cccctctacc tgatcaatca accgctgttg gtcattttgc
     2101 gccatattgc gaagcattgc agtgacgata atctccaaag cttctacctg cgcacacagt
     2161 tctttcgact cttcttcttt ttgggcaaac ttaaataaca actcagcaat gagatttttc
     2221 atgtctgtat ttccttatcc aaagtatgga gaagttaaca ttatgtttca caacttcata
     2281 gtgtctggaa gtattatttt tatgacaaag gaaatacttt gcgcaaatat tttttgtttg
     2341 attgccagcg aaacgtttct cttatttaaa taattaccca cacaaaatat aggcttttaa
     2401 ttatcaacct ttattataaa taaaagctat gaaatatatt ctcttcgtta acttataatt
     2461 aagagaaaaa aactcaccgc attgccattt aagccaggaa taaattagct tcccgttcat
     2521 catttttgtg atcttgcgca catctttgag ataacgcact ttcttcacca gtacgttgca
     2581 cgctttgtca gcagaagatt gcaacaatct ttcagaaggc cgcgaatccc ccgttttgtc
     2641 gtggtaatgt atgcactttg tgttagacgg gataggtttt taagatggaa aaactgcggg
     2701 taggaatcgt ttttggtggt aaatcagcgg aacatgaagt gtctctgcaa tcggcaaaaa
     2761 acattgtcga tgccattgat aaaagtcgct tcgacgttgt gctgctgggc attgataaac
     2821 aagggcaatg gcacgtcagc gatgccagca attatctgct aaatgcagac gatcctgccc
     2881 atattgcgtt gcgcccttcg gcgaccagcc ttgcgcaggt gccaggtaaa catgagcatc
     2941 agcttatcga cgcgcaaaac ggtcagccgt tgccgacggt ggatgtcatt ttcccgattg
     3001 tccacggtac gctgggcgaa gatggttcct tgcagggaat gctgcgggtc gccaatttac
     3061 cgtttgtagg ttctgatgtt ctggcttcag cagcctgtat ggataaagat gtcaccaaac
     3121 gtctgctacg cgatgccggg ctgaacattg cgccatttat taccctgacg cgcgctaatc
     3181 gtcacaacat cagttttgcc gaagtggagt ctaaactggg gttaccactg tttgtaaaac
     3241 cggctaatca gggctcttct gttggtgtca gcaaagtaac cagtgaagaa cagtacgcaa
     3301 ttgccgtcga tctggcgttc gagttcgatc ataaagtgat cgttgagcaa gggatcaaag
     3361 gtcgtgagat cgaatgcgca gttctgggca acgacaatcc gcaagccagc acctgtggcg
     3421 agatcgtact caccagcgat ttctatgcct acgacaccaa gtacattgac gaagatggcg
     3481 cgaaagtggt agttccggca gccattgcac cagagatcaa cgataagatc cgggcgattg
     3541 ccgttcaggc ttatcagacg ttgggatgcg caggcatggc gcgtgtagac gtgtttttaa
     3601 ccccagagaa cgaagtggtg atcaacgaga tcaacacact gcctggcttc accaacatca
     3661 gtatgtatcc gaagctgtgg caagccagcg gtctgggtta caccgatctg atcacacgtt
     3721 tgattgagct ggcgctggag cgtcacgctg cggataacgc attgaaaacc acaatgtaat
     3781 attttaacgc ccgataatat tcttattggg cgttttcagt ttcttcatcg cgacgacgaa
     3841 taatcaaccc cgccagccag aaactaatca cccacgtcac cagaccgacg gcataggtct
     3901 gccaaccttt tgcttcaaat cccagtactc ccaccacgcc attaagaatg aatataaggc
     3961 caatggcgaa cgcatagtag tgccagtcac ggcggatttt tacaggcaga ttcatggcag
     4021 ctccgttaac gaaaaacaac atccttgcat attctgtaac aaccgtctgt ggtgtagaca
     4081 acattttcgt aattattaat aaatttaact taaggttgcc attttgtgac tcacaacgaa
     4141 gattcacgct tcgggaatat tcccaacatc aatttaccat gattctgata ttgacaatct
     4201 aaatgacctg tcagacttgc cataagccgc tggtatcaca ccagaagaga agcagatgac
     4261 ttccggaggt ttttatggct gatttcaccc tgtcaaaatc gctgtttagc ggaaaatatc
     4321 gcaatgcctc ttcaacgcct ggcaacattg cctatgcgtt gtttgtgctg ttttgctttt
     4381 gggctggggc gcaattgctg aacctgttag tgcatgcgcc cggcgtctat gagcgtttaa
     4441 tgcaggtcca ggaaacaggt cgcccacggg tggaaattgg tttaggtgtc ggcaccattt
     4501 tcgggctgat cccgttttta gcaggctgcc tcatttttgc agtggtggcg ctatggctgc
     4561 actggcgaca tcgccgccag taaaatttac gttgccagat gcggcctgaa acgccttatc
     4621 tggcctgaca ttcctgtttg atcactgttt catacaacgc gcccgccgtt cgtctacgcg
     4681 cttcgcaaac caggccgtag tcaggttgcg ggtgattttc gggctttcca gttgaatgcc
     4741 aggcaacatt tcgcggggta gtgatttacc cgttttcgct tcggcaagtt gataaacttt
     4801 cttgtacagc gccgtctcct caaaagagaa gctgtcacct ttctctaact gacggcgaat
     4861 ttcgctgtcg ctcatcccca gttttcccgc cagtttgcgc gttgccagtt cggttttccc
     4921 gggttcttta ctgtcgtaac gaattaaatc accgtccagt gccagcttca cgccgctggc
     4981 cttactgact gcgttctgaa acgcggcatt acggctggcg taccagccag cgttaaaatc
     5041 agcaaagcgg tatatcggtg cgctatagct ggcgggataa ttcagtaaat ggtaagtacc
     5101 aaaccacaac ccgccgcggc gactgaagac ttcctgacga actgtaccgt ccattttcca
     5161 cggataccct ttggtatgct gttcggcaaa agcaatgctg acctgcatcg gcccaccggt
     5221 gcgcaccgga ttaagcgaac caaacagcgt ctgccccatt ggtaccatgc tgatcagatc
     5281 gtcaaaaatc gcgctcaatt gcttttctgt acgcaccgaa tctaaccgtt cgctataact
     5341 tttgccgttt ggcgatttga tttttagcgc ggtatgaacc agaaaagcag gaatgtgcat
     5401 ccgttcagca cgacggtcaa tctcttgcca ggcgatcttg cttaagcccg gaacggccgg
     5461 atctgcctga taatttgact cctgctgcgc caccgccagc accgagtaga cgttttccag
     5521 cgtcggtgcc agcccctgac tttcaaaggt ggtggcgaga tctttcgccc aggcatcgcg
     5581 gtctttcacg ctcgcaggca tcttctgccg cacgacgctc gctacatcta tcgctttttc
     5641 gccctttttc aacggttgcg gagcctgact actacacccc gctaaaacca gaaccgccaa
     5701 caatgacagc gaagatagat gattgacgcg cgacattact tctccttttt agctcaaggt
     5761 atgggttact tcctgaattt tgtcaccatc cagctcatgt tcaaagctgc gcagacgttt
     5821 gtagatagac atcaactcaa ccagtgtggt ccatgagtta atcaggtact ggaaagcacc
     5881 gcgaacctga ccaaaaacgt tggtaatctg cgtcatcagg ccgagcgtaa tcgtaccggc
     5941 aacaattgac ggaaacagca agaacaaacc gaaaacgtta tcgacctgca aatagagaat
     6001 gcgggcgatg ttgaaataca tatagtgaaa atagaggcga aaatagtttt tccgtacggc
     6061 gctaaacagc tcgcgtaccg taggcggcgt cgcgcgcgtg gcatcgtctt caccataaac
     6121 cagctcttta cggtaggcgg cctctactcg ctggttttta aactccagcc ccggcagttt
     6181 gatccctacc actgccagca atccggtccc catcagcgac cagacgatag cggcaatcac
     6241 cagaccatac ggaatgtgcc cgataatcgg cagctccggc acatgcgcgg agagcgttac
     6301 cagcaccggc aggaaggcga tcaacgtcat gatggcgttg ataaaactga cgcccatatt
     6361 ctccagcgtt gaagcaaaac gcatggtgtc ttcctgcaca cgctgtgcgg caccttcgat
     6421 atgacgcagt tgttgccagt tcgccatgta atattcgttc atcgccgtac gccagcggaa
     6481 cacgtagtga ctgacaaaga agttgttcag tacactgatc accaccgcga tcagcgcaat
     6541 ccccagaaag acgcccactt cgcggtaaaa ttgttcgata gtgactttat gcggcgaact
     6601 tagcgcggtt tgaatcagat catagaacgg cgcataccag gcgttgacgg cgaccccgac
     6661 ttccaccaaa aaccaagtga cgaagataat cagcgcagta ccgagtattg accagtattg
     6721 ccaacggtgc gggctgtaga taaaccagaa aaatgcaaaa agtcctacgc aaacaatgta
     6781 gtaagcgtaa aaaatcagga aatccaacga ccagaaacgc gcggcgctaa tcgggatctg
     6841 cccggaagcg ccggtgatac gtgccaccca gtcaccccca ccggcttgcc agaagataac
     6901 ggcaatcaat gcccaaacaa aggccgagag aaaaaacgtt cccggctttg ggaaaaaaga
     6961 cttaaacata tgcactcctg ctaacttctt atcgttttaa caactgctgt gtattaaccg
     7021 catgaccgct acaaagcgtc agccaaatga aaccgcccat tgtgacccat agcaaggcgc
     7081 ttagttcgcc agcctgacca taagaattgt ttcgacggat ttcacaaacc aaagattgtt
     7141 tacataagcg gattaacacg cgcaggatca caaatgcagg cattggtgag caggaaaaag
     7201 atcgtgttta cgcgccgtta cgtttttaac actattattg cactaattcg ccctttgcaa
     7261 tccatcaatg agtagtataa atacgctcag ttaccttcat tcaatctatg gacaccaccg
     7321 ttgaaacgta gtctgctttt ttctgccgtg ctgtgtgcgg cgtcattgac ctccgtccat
     7381 gcggcgcagc cgatcactga accggagttt gcctctgata ttgtcgatcg ttatgccgat
     7441 catatttttt acggcagcgg tgccacgggg atggcgctgg ttgttatcga cggtaaccag
     7501 cgcgtctttc gtagttatgg tgaaacgcga cctggtaata acgttcgccc gcagctggat
     7561 tcggtcgtgc gtattgcttc cctcaccaaa ctaatgacca gtgaaatgct ggtgaaattg
     7621 ctcgaccagg ggaccgtaaa gttaaacgat ccgctaagta aatatgctcc gccaggcgca
     7681 cgcgtgccga cctacaatgg gacgcccatt accctagtga atctggcaac ccataccagc
     7741 gccctgcccc gtgaacagcc cggtggcgcg gcacatcgtc cggtgtttgt ctggccaacg
     7801 cgcgagcaac gctggaaata cctttctacg gcgaagctga aagccgcgcc aggcagtcag
     7861 gcggcgtact ctaaccttgc gttcgacctg ctggcagatg cactggcgaa tgcgtccggc
     7921 aagccttata cccagttgtt tgaagagcag attacccatc cattggggat gaaagacacc
     7981 acctacaccc cctcaccgga tccgtgccgc cgtttaatgg ttgctgagcg cggtgccagt
     8041 ccgtgcaata acacgctggc ggcaattggc agcggcggcg tctattccac gcctggcgat
     8101 atgatgcgct ggatgcagca gtatttgtca tctgattttt atcagcgcag taaccaggca
     8161 gatcgcatgc agacgttgat ttatcagcgc gcgcagttta cgaaagtgat tggcatggat
     8221 gtccccggca aagcggatgc gcttggttta ggctgggtat atatggcccc gaaagagggt
     8281 cgtccgagga ttattcagaa gacaggcggt ggtggcggtt tcattaccta tatggcgatg
     8341 atcccgcaga agaatattgg cgcgtttgtg gtggtcactc gttcgccgtt gacgcgcttt
     8401 aaaaatatga gcgatggcat caatgatctg gtgaccgagc taagcgggaa taaaccgttg
     8461 gttatccccg catcctgatt aatactctga aggcaaacgg ttgatagcga ccagtttgcc
     8521 tttattcatt tcgatatagc cgcctttacg caaagcagcg agaacttctg cgacgacaga
     8581 acgcgatatg cgtgtacgtt gatggatata attcatcaca ccaatacgtg atcgcaattc
     8641 ttcattccag tcaatcattg ataataatgt tgcgcgggtt tgtttggctg gtagatgata
     8701 tcccgtacaa tttccttctg atattaattt gtatggtata tcgtttttca ttaaaccatc
     8761 agccagcccc ataatataag gagcctgggt aataccgata agtacgtttt cttctctacg
     8821 cagagagata actccttcta aaataacgaa agtatcttcg ttattgacat cactggaaaa
     8881 tataacttgc ttttcattat taaactcgaa gcgcgtaccg tatctggaca aacatttatc
     8941 gagcttacca aattcctgaa gaggtttaac tacagataac atttgcgcgt cctttgcagt
     9001 aatgcccgtc aaatccttga cgggcattat ttagattaaa ttaccagtat ttcttcggag
     9061 tgaagaatat taccaagtat atttaacacc cacgttcgcg gaccagtctt gatctacgtc
     9121 accaccaccg aggtagttag catcggtata ggcgctgaag ttcttggtga agctgaactg
     9181 agtacccaga ccaacacgta ccgcagaccc ttcagtaccg ttatcgatgg aatcaccgtt
     9241 cacatcgtta tcgttgttag agtcgtcgta gacgtaagcc agtttgaagt acggagtcag
     9301 agcctgatct tcgctgtagg tgaaggtata acctgcatct acacccagtt cataacgcat
     9361 gctgtcgtaa ggctgaccgt caactttcat gtcgttgctc agctggtagt catcaccaga
     9421 ctggaacaga ccagaaatgc tgccgtaagg agtcacgtaa ccagcatcac ccagtttgaa
     9481 gtcgtaaccg gttttcaaac cgaagcccca agcgtcggag ttggtgctac cgtcaacgta
     9541 agtaccgttg ctcatggttg cagacaggtc gttgttgaag tgagagtagc tcaag
//
LOCUS       DAJTSA010000135         9572 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000135 DAJTSA010000000
VERSION     DAJTSA010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.135"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..133
                     /locus_tag="OO369_003622"
     CDS             <1..133
                     /locus_tag="OO369_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5983751.1"
                     /translation="SNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQTYMPRV"
     gene            230..382
                     /gene="hokE"
                     /locus_tag="OO369_003623"
     CDS             230..382
                     /gene="hokE"
                     /locus_tag="OO369_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR5983752.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            733..885
                     /gene="hokE"
                     /locus_tag="OO369_003624"
     CDS             733..885
                     /gene="hokE"
                     /locus_tag="OO369_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR5983753.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(1007..1627)
                     /gene="entD"
                     /locus_tag="OO369_003625"
     CDS             complement(1007..1627)
                     /gene="entD"
                     /locus_tag="OO369_003625"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR5983754.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1802..4042)
                     /gene="fepA"
                     /locus_tag="OO369_003626"
     CDS             complement(1802..4042)
                     /gene="fepA"
                     /locus_tag="OO369_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR5983755.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4285..5487
                     /gene="fes"
                     /locus_tag="OO369_003627"
     CDS             4285..5487
                     /gene="fes"
                     /locus_tag="OO369_003627"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR5983756.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5490..5708
                     /gene="ybdZ"
                     /locus_tag="OO369_003628"
     CDS             5490..5708
                     /gene="ybdZ"
                     /locus_tag="OO369_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR5983757.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5705..9550
                     /gene="entF"
                     /locus_tag="OO369_003629"
     CDS             5705..9550
                     /gene="entF"
                     /locus_tag="OO369_003629"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCR5983758.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDI
                     ISPGAFVKIGPIIRATLNR"
BASE COUNT         2105 a         2505 c         2630 g         2332 t
ORIGIN      
        1 cagtaatata agcgaactga aggatgctgt tacggaatat attgaatact ataacagcag
       61 aagaattagc ctgaaattaa aaggtctgac tccaattgaa tatcggaatc agacctatat
      121 gcctcgtgta taactgtcca actttttggg gtcagtacac cctggggctg ccactccaaa
      181 ccagacaatt tggatggtag tcccttcttc gcatggaggt aatataaaca tgctgacgaa
      241 atatgccctt gtggcagtca tagtgctgtg tttgacggta ctgggattta cgcttctggc
      301 cggagactcg ctgtgtgagt ttacggtgaa ggaacgtaat attgagttca gggctgttct
      361 cgcttacgaa ccgaagaagt agccgttgtg cggggagtaa tccccgcata tccggttgtc
      421 aggtcaggat ggtaaggcac ctgctttaca ctttcgcccg cggtcagtga tggctgcggg
      481 cgatttcgtt ttaagagcaa tgatgagttt tgccgacaga agtccgaaac aggactcact
      541 gtataaataa acagctattt tgctaaggaa gggtaagata acggcgggtg cctgaagctt
      601 tccggtttca ggttaactct gaggtttgga aagatgaagc cccaagagat atttctatca
      661 atcctggggc tgccactccg aacctcaaca actcggatgg tagtcccttc ttcgcatgga
      721 ggcaatataa acatgctgac gaaatatgcc cttgtggcag tcatagtgct gtgtttgacg
      781 gtgctgggat ttacgcttct ggtcggagac tcgctgtgtg agttcaccgt aaaggaacgt
      841 aatattgagt ttaaggctgt tcttgcttac gaaccgaaga agtagccgtt gtgcggggag
      901 taatccccgc atatccggtt gtcaggtcag agtggtaagg cacctgcttt acactttcgc
      961 ccgcagtcag tgatggctgc gggcgaatcg taccagatgt tgtcaattaa tcgtgttggc
     1021 acagagttat gactattttt tcttttatct gccagtgcac agcaaacatc tcattctcac
     1081 gatgaatgat gacctgctgt ttattccagc taattatctg atagtccaga aaacctgcat
     1141 cagtttggat ctcacttgcc ttaaatgcgc tctctttggc ggaaaatgca agtgtcagcg
     1201 ccagagaaaa ggctaaaccg cagtctgcga gtcgctcgtg ttctgctggt gtaataatgt
     1261 tgtctgtcaa ttcggtcgct gtttgcgcag aaaaaatttc ttcgatatca acgccaatcg
     1321 gttgacggga aaccacggcc aatgccgtag ccccgcagtg gctaatactg ccgtatacct
     1381 ccgcaggcca caccggttgg cgtagctcgc cgattgcggg cacacattta tagccatatt
     1441 cccgcaaggc ataaacagca gcgatccgtc cggctaaatg ctctgtttta cgtttacgtc
     1501 cagcgtgttg cagttgtgcg tagtgcggca gccagagtaa atcctgctca caaaaattcg
     1561 ccggatcgaa ctcaacaaaa tgcagcgtat gtccggcaaa ggggagggag gtatgcgtag
     1621 ttttcatatc gaccatattc gagactgatg acaaacgcaa aactgcctga tgcgctacgc
     1681 ttatcaggcc tacgtgatcc tgcaatatat tgaattggca agatctttgt aggccggata
     1741 aggcgttcac gccgcatccg gcgtgaacga cgcgcacttt gtcaacaatc tgacgttagc
     1801 atcagaagtg agtattaatg ctcatatacc acgtacgtcc cggctcgtta taggtatacg
     1861 cgccggcacc ggcgatatag ttggctcctg ccagatcgcc cgtggtctgg gcattacctg
     1921 cacgccacaa acgtttgtcg aacaggttgt ccacgccgcc ggtcagactg acattcttcg
     1981 tcacatccca ggtcgcgctc agacccacaa tgctgtaagg actgatttct ttggtttccg
     2041 ggccaaccgc tggctgacct ttatagttgt acttcttcgg ctgctgcttg ccgtaccagg
     2101 tgaaggtcgt ttgcatcgac aaatcttccc gtgcctgcca gctcagcgtt gagttcaacg
     2161 tgtactccgg gatgatcgac aaacggtcgc ccgtggtttt gttttcactc ttcagcatat
     2221 aagtgatgtt attggtccac atcaccgttt cgctaactgg tacgtttaac gatccttcca
     2281 gaccttcaac cactgctttc ggcacgttat cccactgata gagatcagtg ccgactgcgt
     2341 tttgccctac agccacatag cccgcttcaa tcttattgcg ataatcgtta cggaaccagg
     2401 tgacgcccgc cagccagccg tcgcgtttga actccagacc aatctctttg ttgatgctgg
     2461 tttctacttt caggtcatcg ttaccttgca gatagcagcc gcccgcgctg gcatagcagc
     2521 cctgaccttt actgtagaga atgtagttcg ggttagtctg gtataggctc ggcgctttat
     2581 aggcgcgggc aatgcctatt ttcagcgtga agtcatcgcc taaaccttgc gatatgttca
     2641 gcgccgggct ccagttattg ccgacaatac tgtgatgatc gaaacgcagc cccggcgtta
     2701 cgatggtgct gtcagtcagc tccatgttgt tttcggcaaa cagcgagaaa atttctgctt
     2761 ttgaatacgg gctacggtcg gtggcactca cgccatcaat agcgccaccg gtattcgttc
     2821 cggtcagtgc ttgggtgttg gaacttaagt ccttcatccg ttgctgattc cactccgtac
     2881 ccagcgtcag cgtctggtta acgaggaaat caatcggcag gttaacttcg ctgtgcagca
     2941 tcacgtcatc gagatcgata tcgacgaaat cctgtgtcgc tttttcgtta aatttccctt
     3001 cggtaccgcc cgccagacct tccggaatac gcgagttacg ggtgtgttca tactgcaccc
     3061 agttgctggt ggtcacgccg ttatcccagc caccgttcca ggtcagcgcg tagttctggc
     3121 gatacaggcg gttggtttca tcgccatatt tcgagcgagt gtaagcgtca gagttggtgt
     3181 tctgggtatc gcccgcatac aggttaccct ggcggctgta acccgcttcc agttccagcg
     3241 attgcaatgg tgcgaagtcc cagcgcacca cgccgttaat atctttattg atcacccctt
     3301 cgcgcccggc tggtaacgtc gtggcatacg ttccggcacg cgcggactga tggccctggt
     3361 tgatatccca cgcgtcagcc tgagttttgt cgaggttgcc atacaagcgg aagctgaatt
     3421 cgtcgcccag cggaccggtc aggctaaagt tagtgcgttt ggtggcacct tcctctttat
     3481 gttctggcgc attgaaatat gcgtcccagg agccgtgcca ctcgccgctg ccttttttgg
     3541 taatgatgtt aaccacgccg cccgccgcgc cgttgccata acgcgcagct gccggaccac
     3601 gcagaacttc aatacgttca atcatttcag gtggcaccca ggaggtatca ccacgggtat
     3661 cgcgctcgcc acgccagccc tgacgcaccg agttacggct gcttaccggc ttgccgtcaa
     3721 tcaaaatcag cgtgttttcc ggacccatac cgcgaatatc aatctgtcgg ttattcccac
     3781 gctgaccact ggtggagtta ccggtcaggt taacgcctgg catggtacgg atgatctccg
     3841 acacatcgcg ggcaaccggg tttttgcgga tttcatctgc ggtgatggtc gaaacgccag
     3901 gagcctgcaa gttctgctcg gcggcggtaa cgacaatggt atcgtcatgt gaaacaggtg
     3961 tatcggtcgg ctcttgtgcc tgcgctaccc cataaatccc cagattgacc aacaaggcca
     4021 gggaatgaat cttcttgttc attgttttat tcctgcattt ttgccacgaa ttgcaactgt
     4081 cgggcatggt cgtcatcaac acgacgcatc ccgttaccgc gaaaaccttt gatcctgaaa
     4141 gacacgcagt gcagttggtt aattaatgtc cgcgcttccc acggcgcgcc attacgctat
     4201 tgcaaatgca aatagttatt aataacatta tcaatatatt tctgcaatca atgaaaaatt
     4261 gcacagtaaa catggggtta tggtgtgacg gcgttaaaag taggaagtga gagctggtgg
     4321 cagtcgaaac atggcccgga atggcagcgt ctgaatgacg aaatgtttga ggtcactttc
     4381 tggtggcgtg atccccaagg ttctgaagaa tactcgacga taaagcgcgt atgggtctac
     4441 atcactggtg tgaccgatca tcatcagaac agccagcccc agtcgatgca gcgaattgca
     4501 ggtactgatg tctggcagtg gacgacacaa ctcaatgcca actggcgcgg cagctactgc
     4561 tttattccca ccgaacgcga tgacattttt tctgcaccat cccccgatcg cctcgaatta
     4621 cgtgaaggct ggcgaaaact attaccccag gcaatagccg atccgctgaa tccacaaagc
     4681 tggaaaggcg ggctagggca cgctgtttct gcactcgaaa tgccgcaagc gcctctgcaa
     4741 ccgggatggg attgtccgca agcgccagaa ataccagcca aagaaattat ctggaaaagt
     4801 gaacggttga aaaattcacg tcgtgtatgg atttttacca ccggcgatgt aacagcagaa
     4861 gaacgcccgc tggcagtttt gctcgatggc gaattttggg cgcaaagtat gcccgtctgg
     4921 cctgtgctga cttcgctgac ccatcgtcag caacttcctc ccgccgtgta tgtgttgatc
     4981 gacgctatcg acaccacgca ccgcgcccac gaactgccgt gtaatgcgga tttctggctg
     5041 gcagtacagc aagagttatt acccctggtg aaagttattg ccccttttag cgatcgcgcc
     5101 gatcgcaccg tggtcgccgg gcagagtttt ggtgggctgt ccgcgctgta tgccggactg
     5161 cactggcctg aacgctttgg ctgtgtatta agccagtcag gatcgtactg gtggccgcat
     5221 cggggcgggc agcaagaggg cgtgttactt gaaaagctaa aagctggtga agttagcgcc
     5281 gaaggtctgc gcattgtgct ggaagcgggt attcgcgagc cgatgatcat gcgggccaat
     5341 caggcgctgt atgcgcaatt acaccctata aaagaatcca ttttctggcg tcaggttgac
     5401 ggcggacatg atgcgctttg ttggcgcggt ggcttgatgc aggggttaat cgacctctgg
     5461 caaccacttt tccatgacag gagttgaata tggcattcag taatcccttc gatgatccac
     5521 agggagcgtt ttacattttg cgcaatgcgc aggggcaatt cagtctgtgg ccgcaacaat
     5581 gcgccttacc ggcaggctgg gacattgtgt gtcagccgca gtcacaagag tcctgccagc
     5641 agtggcttga agcccactgg cgtactctga caccggcgaa tttcacccag ttgcaggagg
     5701 cacaatgagc cagcatttac ctttggtcgc cgcacagccc ggcatctgga tggcagaaaa
     5761 actgtcggat ttaccctccg cctggagcgt ggcgcattac gttgagttaa ccggagaggt
     5821 tgatgcgcca ttactggccc gcgcggtggt tgccggatta gcgcaagcag atactttgcg
     5881 gatgcgtttt acggaagata acggcgaagt ctggcaatgg gtcgatgatg cgctgatatt
     5941 cgaactgcca gaaattatcg acctgcgaac caatattgat ccgcacggta ctgcgcaggc
     6001 attaatgctg gcggatttgc aacaagattt gcgcgtcgat agcggtaaac cactggtctt
     6061 tcatcaactg atacaggtgg cagataaccg ctggtactgg tatcagcgtt atcaccattt
     6121 actggtcgat ggcttcagtt ttccggccat tacccgccag atcgccaaca tttactgcgc
     6181 attgctgcgt ggcgaaccaa cgcctgcttc gccgtttacg cctttcgctg atgtagtgga
     6241 agagtaccag caataccgcg aaagcgaagc ctggcagcgt gatgcggcat tctgggtgga
     6301 acagcgtcgc caactgccac cgcccgcgtc actttctccg gcacctttag cggggcgcag
     6361 cgcctcggca gatattttgc gtctgaaaat ggaatttacc gacggggaat tccgccagct
     6421 ggctacgcaa ctttcaggtg tgcagcgtac cgatttagcc cttgcgctga cggctttgtg
     6481 gctggggcga ttgtgcaatc gcatggacta cgccgccgga tttatcttta tgcgtcgact
     6541 gggctcggcg gcgctgacgg ctaccggacc cgtgctcaac gttttgccgc tgggtattca
     6601 tattgcggcg caagaaacgc tgccggaact ggcaacccga ctggcaaccc gactggcagc
     6661 acaactgaaa aaaatgcgtc gtcatcaacg ttacgatgcc gaacaaattg tccgtgacag
     6721 cgggcgagcg gcaggtgatg aaccgctgtt tggtccggta ctcaatatta aggtatttga
     6781 ttaccaactg gatattcctg gtgttcaggc gcaaacccat accctggcaa ccggtccggt
     6841 taatgacctt gaactggccc tgttcccgga tgaacacggt gatttgagta ttgagatcct
     6901 cgccaataaa cagcgttacg atgagccaac gttaatccag catgctgaac gcctgaaaat
     6961 gctgattgcc cagttcgccg cggatccggc gctgttgtgc ggcgatgtcg atattatgct
     7021 gccaggtgag tatgcgcagc tggcgcagat caacgccact caggttgaga ttccagaaac
     7081 cacgctgagc gcgctggtgg cagaacaagc ggcaaaaaca ccggatgctc cggcgctggc
     7141 agatgcgcgt tacctgttca gctatcggga aatgcacgag caggtggtgg cgctggcgaa
     7201 tctgctgcgt gagcgcggcg ttaaaccagg ggacagcgtg gcggtggcac taccgcgctc
     7261 ggtctttttg accctggcac tccatgcaat agttgaagct ggcgcggcct ggctaccgct
     7321 ggataccggc tatccggacg atcgcctgaa aatgatgctg gaagatgcgc gtccgtcgct
     7381 gttaattacc accgacgatc aactgccgcg ctttagcgat gttcccaatt taaccaacct
     7441 ttgctataac gccccgctta ccccccaggg cagtgcgccg ctgcaacttt cacaaccgca
     7501 ccacacggct tatatcatct ttacctctgg ctccaccggc aggccgaaag gggtaatggt
     7561 cgggcagacg gctatcgtca accgcctgtt gtggattcaa aatcattatc cgctcacagg
     7621 cgaagatgtc gttgcccaaa aaacgccgtg cagttttgat gtctcggtgt gggagttttt
     7681 ctggccgttt attgccgggg ctaaactggt gatggctgaa ccggaagcgc accgcgaccc
     7741 gctcgctatg cagcaattct ttgccgaata tggcgtaacg accacgcact ttgtgccgtc
     7801 gatgctggca gcatttgttg cctcgctgac tccgcaaacc gctcgccaga gttgcgtgac
     7861 gttgaaacag gttttctgta gtggtgaggc cttaccggct gatttatgcc gcgaatggca
     7921 acagttaacc ggcgcgccgt tgcataacct gtatggcccg acggaagcgg cggtagatgt
     7981 cagctggtat ccggcttttg gcgaggaact ggcacaggtg cgcggcagca gtgtgccgat
     8041 tggttatccg gtatggaata cgggtctgcg tattcttgat gcgatgatgc atccggtgcc
     8101 gccgggtgtg gcgggtgatc tctatctcac cggtattcaa ctggcgcagg ggtatcttgg
     8161 acgacccgat ctgaccgcca gccgctttat tgccgatcct tttgctcctg gtgaacggat
     8221 gtatcgtacc ggagacgttg cccgctggct ggataacggc gcggtggagt acctcgggcg
     8281 cagtgacgat cagctaaaaa ttcgcgggca gcgtatcgaa ctgggcgaaa tcgatcgcgt
     8341 gatgcaggcg ctgccggatg tcaaacaagc cgttacccac gcctgtgtga ttaaccaggc
     8401 ggcagccact ggtggtgatg cgcgtcagtt ggtgggctat ctggtatcgc aatcgggcct
     8461 gccgttggat accagcgcat tgcaggcgca gcttcgcgaa acattgccgc cgcatatggt
     8521 gccagtcgtt ctgctgcaac tgccgcagtt gccacttagc gccaacggca agctggatcg
     8581 caaagcctta ccgttgcctg aactgaaggc acaagcgcca gggcgtgcgc cgaaagcggg
     8641 cagtgaaacg attatcgccg cggcattcgc gtcgttgctg ggttgtgacg tgcaggatgc
     8701 cgatgctgat ttctttgcgc tgggcggtca ttcgctactg gcaatgaaac tggcagcgca
     8761 gttaagtcga cagtttgctc gtcaggtgac gccggggcag gtgatggtcg cgtcaaccgt
     8821 cgccaaactg gcaacgatta ttgatggtga agaggacagc tcccggcgca tgggattcga
     8881 aaccattctg ccgttgcgtg aaggtaatgg cccgacgctg ttttgtttcc atccggcgtc
     8941 cggttttgcc tggcagttta gcgtgctctc gcgttatctc gatccacaat ggtcgattat
     9001 tggcattcag tcgccgcgcc ctcatggacc catgcagaca gcgacaaacc tggatgaagt
     9061 ctgcgaagcg catctggcaa cgctacttga gcaacaaccg cacggcattg cggcgcggct
     9121 gcgtgcccgt ggcgaacagg tggcatttct tggtttgctg gatacctggc cgccagagac
     9181 gcaaaactgg caggaaaaag aggctaatgg tctggacccg gaagtgctgg cggagattaa
     9241 ccgcgaacgc gaggccttcc tggcggcaca gcagggaagt acttcaacgg agttgtttac
     9301 caccattgaa ggcaactacg ctgatgctgt gcgcctgctg acgactgctc atagcgtacc
     9361 gtttgatggt aaagcgacgc tgtttgttgc tgaacgcacg cttcaggaag gtatgagccc
     9421 cgaacgcgcc tggtcgccgt ggattgcgga gctggatatc tatcgtcagg attgtgcgca
     9481 tgtggatatt atctctccag gggcgtttgt aaaaattggg ccgattattc gcgcaacgct
     9541 aaacaggtaa attaatatta tctataaacc ca
//
LOCUS       DAJTSA010000136         9520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000136 DAJTSA010000000
VERSION     DAJTSA010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.136"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..591)
                     /locus_tag="OO369_003630"
     CDS             complement(<1..591)
                     /locus_tag="OO369_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HCR5983759.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQ"
     gene            complement(658..1383)
                     /gene="fimC"
                     /locus_tag="OO369_003631"
     CDS             complement(658..1383)
                     /gene="fimC"
                     /locus_tag="OO369_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5983760.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1420..1959)
                     /locus_tag="OO369_003632"
     CDS             complement(1420..1959)
                     /locus_tag="OO369_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5983761.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2024..2572)
                     /gene="fimA"
                     /locus_tag="OO369_003633"
     CDS             complement(2024..2572)
                     /gene="fimA"
                     /locus_tag="OO369_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5983762.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(3053..3649)
                     /gene="fimE"
                     /locus_tag="OO369_003634"
     CDS             complement(3053..3649)
                     /gene="fimE"
                     /locus_tag="OO369_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR5983763.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(4127..4729)
                     /gene="fimB"
                     /locus_tag="OO369_003635"
                     /pseudo
     CDS             complement(4127..4729)
                     /gene="fimB"
                     /locus_tag="OO369_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            6183..6899
                     /gene="nanC"
                     /locus_tag="OO369_003636"
     CDS             6183..6899
                     /gene="nanC"
                     /locus_tag="OO369_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR5983764.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHADETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWETENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            6919..8025
                     /gene="nanM"
                     /locus_tag="OO369_003637"
     CDS             6919..8025
                     /gene="nanM"
                     /locus_tag="OO369_003637"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR5983765.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            8090..9070
                     /locus_tag="OO369_003638"
     CDS             8090..9070
                     /locus_tag="OO369_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR5983766.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2788 a         2076 c         1997 g         2659 t
ORIGIN      
        1 ctgtacacta tttccgctga aattataatt gagcaatccg gcattaatac cgggatccca
       61 taactcagga ggaatataac cacgcgcgcg attactcata aatgcctgag ggatcgtcag
      121 gttcagtcgc tgctgaccaa catccagatg cgcagtagcg tcctggacca ttgtggttaa
      181 tggcacacag gcatcatccg ccagcagatt cataccggcg acagaagccg tattcagccc
      241 catactggcg agttgcgcgc gtgtcaggca gggaacaatc ccttgttcac tgtcgcccgt
      301 attaaatgtg acatcacgcg ttgccatata accattattc aaatagatat cgacgcgata
      361 cgtccctggc ggtaattctt gcccattttc aaaacgcgat aaatcggcca cagcctgggg
      421 atcatccgct aaaaagcgca gattaaaata gaggtcggca gatgacaaag gtgcctgtgc
      481 ggcaaaagca caggcgacaa cgagtcggac aaaaaaacca gccaaacgat gcttacgaat
      541 atgcaagcat tgtgtgtttc gctggtaaag tcttaaattc agatatgaca ttattcctcc
      601 ggtttaaaat cacccggcag tcaattcttt ttattcaggc gaaaaattcc ccctgcgtta
      661 ttccattacg cccgtcattt tgggggtaag tgcgccataa tcatttattg ttcggtaagt
      721 aatattgctt cctgcatcag aaggcaattt aaccgtgctt tcgcccattg gaggcaccaa
      781 tgcattttca agaacccggg ttccggcatt caactctgtt accgtcaggt aatagggtgt
      841 cgggttaatc agcgtcagag aattcgcgct acgacgaaat cttaattttt ctgcggcctg
      901 atcgggtggc aacgctaatt tagccgggcg atagtacagt ttaatgcggc tgataattgc
      961 gagctgtagc atattctcag tcaattttga tttatccatt gacggaatcg ctttaacgtt
     1021 catccagaat aaactttccc ggtcctgtgg caattggtta tttgttgcat caagaatacg
     1081 taaggtattc tctttttttc ccttcatcgc aaacagagga ggcgtcacga taaaacgacc
     1141 atcctttaca ccatcggcat tttccaccca tgattgaatt aaataggtac tattttcatc
     1201 attatttgtc acggcaagtt gcacttgttt ttgccctgcc ggataaatta cgcgagtcgc
     1261 acctaaggcc actcctgctt cagcgcgtcc ggcaaccatc attgccatga acatcaggat
     1321 acctgccagc aagcagaatg ttatttcctg cgatttcctt acattgacgt ttttattact
     1381 cactgtcctg ttcctgttat cacattatct actgaacaat tactgatagg ttaaagagaa
     1441 ccaggcctgg gcattggcga tgccgccagt aacccgacgc ccggtagcac gatatttggc
     1501 gatgaaatgt agcgaagtag agcctgagta aagccgtttc cagtttgctg gaggacgatt
     1561 aatcggtacg aggtttcctt catcatcaaa caacgctaca ccaatattgg aggctatccc
     1621 tggcccctct cccacggaga gcacatccgg atttttacca tccgcgacac cgtgaaacgc
     1681 cacacccaca cgttcactca ccaccgtgct acattcccgt aaatgaataa caaaaggcac
     1741 cggtgcgcta tcttcccctg ccgcatgaaa ccggttactg ctgatttgcc ccatattgac
     1801 cgtcatttgt ttatcaccgg cttcaatccg gcaagtttcc gcaataatga cgccctgaaa
     1861 ttgcatattt ccgccgggca gcgtggtatt ccatttattt ccggccagag caaacattgg
     1921 cagcaacgac gctaacagaa ataatctctt ccttttcatc gttattttta tcgcacaagg
     1981 gtgggcatcc ctgcccgtaa tgacgtcctt gaacctgggt aggttattga tactgaacct
     2041 tgaaggttgc atccgcatta gctgcacccg gggttgcctc gccgattgca taataacgcg
     2101 cctggaacgg aatggtgttg gtaccgttat tcagggttgt ttgtgcacta aatgtcgcac
     2161 cgtccagcgt cagagcattg cctgttctgt ctaggatctg cacgccaacg tttgttgcac
     2221 tacctgcagc agaactctgc agagccagta catcagtacg cgtagcatca attgccgtac
     2281 ctaagaaggc aacagcggct tttgtggcaa cagtggtatc gcaatcattc agctgaatgt
     2341 taaaaccaac ggcagagctg gttgctccag cctgcttcag gctagcggta cgaacctgtc
     2401 ccaactgaac ggtttgatca acagagcctg catcaactgc gcaagcggcg ttaacaactt
     2461 cccctttaaa gtgaatggtc ccaccattta ccgtcgtagt atcggccaga gccgctgcgg
     2521 aactgaggga cagagccgac agaacaacga ttgccagagt tttaattttc atgctgcttt
     2581 cctttaaaaa aactatttct aaatcgacat gggcagtcgt tctgtacact ttgttttgtc
     2641 aacgagtttg actgccaaca ctgcacagtt ttcccccaaa agatgaaaca tttggggcca
     2701 ttttgactca tagaggaaag catcgcgcac aaactttttc agtttatttg ttggcttaat
     2761 attctattat tatctttatt tatagatttt tatattgcat gaggtggttt ttggagagaa
     2821 gaatgaggaa gatgcgtcga gccacagaaa cgttagcttt acatatagcg gaggtgatgt
     2881 gaaattaatt tacaagagaa ataatttaca tatcaaatag ttagatgctt tttgtcgttt
     2941 tttaatattt ttatgcttga gaaaaaatac gtaacttatt tatgatatgg acagtttggc
     3001 cccaattgtc ttgtgtttat ttgtttttta actttattat caataagtta aatcaaacct
     3061 cttctctttt taatttttcg tttatgagat tatttctttc ccataatccg gcaaaacgag
     3121 cagcattact ggcggtataa cgcacagtat ggcgaatatt tcgatgcccg agataatcct
     3181 gaattaaacg ggtatctgca ccacgctccg ccagttcata accgcaagca tgccttaaca
     3241 tatgaggatg agtctgcgtt acggttccag cttcaatacc ggcatcgcga ataatgcgat
     3301 aggcctgctg gcgagaaagc cgactcccgc ggcgagaaat aaatatagcg tcggtccggt
     3361 cagcgccttt ccagttagca cgttcctggg tccagcgttc cacggcttca cgctcatcaa
     3421 aacgtaacgg gtgaacggta gaaaatccgt tcttcagtcg gcgaatattt attcgacctt
     3481 cattaaggtc aaggtcctga taatgcagat caagcagttc actaatacgc atcccatgcc
     3541 gatatgccaa cagaataaga caataatctc tggctcccgt tgccccgtaa caaaccgcct
     3601 gcatcatggc ctgaacttct ttaccggtaa gataacgacg tttactcaca atagtagtac
     3661 tcctaactga gatatatttg aatgaatacc tataggaaac ctcaatcggt caaaattagc
     3721 ccaatagata aaaagataac cacacacgaa gttatgtggc tatcagtcaa ttacattaaa
     3781 catcatacaa aaataaaata tcactgatgt gacaacatct attttcattg atttgattca
     3841 atataacaat agattacaga ggattaactt ccagttactt aaaaaataaa gaaaaaagtg
     3901 tggaaacagg agaatttaag tgatgtgtaa acttatgaat gattttgttt atttattttg
     3961 aagaacataa atcattagat tttgtatggg tatggttaac ttaatgttta ttatgataat
     4021 taagaatcac acttacaata acatcaatta ataaatggtt aatcattttt gatatatcgt
     4081 aagaataatg tagtttttaa caccatccct ggtatctcaa ctatctctat aaaacagcat
     4141 gacgctgtcg tcctctggct ctatcccaga tgccgtaaaa acgccctgca ttgctggcgg
     4201 tataccagac agtatgacga atattgcgat gcccaagata atcctagata agtcgcgtat
     4261 ctattcccat attcgccaaa gcaaaaccac acgaatagcg taacatgtgc ggatgaatct
     4321 ccagtgacaa cccggcatta ccaccagaag tcgagataat atggtaaaac tgttgccgag
     4381 aaagcggatt ccccttacgt gataaaaata cccactcgct ctcagcatgc ggataagaag
     4441 tacggatact caaccagttt tttaaagcct gaacttcttt gttcaatagc gggtgcgttg
     4501 ttgaaaagcc tttttttaat cgatggatat atatacactt tgctttaaga tcaatatccg
     4561 aaatcctcaa tcgacaaatt tcactcgccc ggaaaccatg aataaaacaa agcaaaatca
     4621 gacaataatt acgtgttgca tgaggcccgg tatttgctgc tttaaggagt gactcgattt
     4681 cactatgggt aaggaagttc ctttttttgt tatcagcctt attcctcatc gttttccctt
     4741 ataattacag acgcgcacta gctgtgctgg gttagcaata atccaacatt tttatcctga
     4801 ttatgtttat aaaagcgaac gtttgcttaa taacttacgt aagtggacca gttcttctga
     4861 gcgaacttaa atggagtagc gatagttact tataataact ttgccataca aacgccatgc
     4921 tcttgcatgc tatgtacctt tatatattta tcaatctgat acgaatctaa taataaccct
     4981 catcattaat gcaactaatc ttatctatat atcatgtgat atgttctgta acaagtaaaa
     5041 tcaacataaa aaattcagca aaatttaata tttgttaaac tagccgtctc aaatatgaca
     5101 ttattagtta actgcatttg ccacaaaatg gctatttcat ggaacgcagt aatttcttag
     5161 tggctattat catgctaatt cttagatccc tagcgattat tcctgcctgg ttcgtcagct
     5221 taggccatga gattgttaca ttccttttta tcctggagat atgggggaac gcaggcgacg
     5281 ctttttcatc tacatccgtt ggtaaatata gctttggtca ctacgactta agccttcctc
     5341 aatcacagct attcgccggg cgatacggca aacaaactaa gtgaaacatc atcctgaaaa
     5401 caccaacatc aacaagcctc tccagatcga catcaggagt gaccagttac aagccacaaa
     5461 ccaagaaaca tatttcatga tgagaattat gctcagcctg atggcgagta aacccttatt
     5521 ttcaccactc tcactgagtt agcttgcaaa gctttctttg tttagattct aaataaatta
     5581 tttataaaac gtatagttaa aagaaaatct ttagtttttg aagaccaccg caagtgttcg
     5641 tctggcttca catggcatct tcttcttaga aaaagatcga catattttgt gacgcgaatt
     5701 gcaaatctgg ttttgttgta tggattgcgt gatttttgat ctggtataac aggtataaag
     5761 atgcaccacg atagtcaatg agactgggca tctcgtaatc tatggcaaac atcacttcag
     5821 ttctttcaca tcgggtgatg aaaatgcact tcagtctgaa aggaatatga aaatgagatc
     5881 aacagacact ctattttatg actctgggta aaatgggttg agtaagtgat ataacttacg
     5941 aatattcaaa tcaattaaaa atcagaagag attttatact caggtattta atctggatct
     6001 ctgtttattt aaataatgtt aaaagagaat tttcacagga gaccttatac aaaaaatata
     6061 aaatacagtt acccgttgcc aaagacacta taagcctggc aaaaaaatat tacacaacat
     6121 aaatgctaat tgtttatgcg ggctttgtat tgctttctgt atcctacaaa tgagtgaaat
     6181 ttatgaaaaa ggctaaaata ctttctggcg tattattact gtgcttttcg tccccattaa
     6241 tttctcaggc tgcgacactg gacgtacgtg gtggatatcg tagtggaagc cacgccgatg
     6301 agactcgact caaagtcagt gagggatggc aaaatggatg gtgggcaagc atggaaagta
     6361 atacctggaa taccattcat gataataaaa aggaaaatgc cgcactcaat gatgttcagg
     6421 ttgaagttaa ttacgcaatt aaacttgatg atcaatggac ggtgcgcccg ggaatgttaa
     6481 cgcattttag cagcaacggc acacgctacg gaccctacgt aaaactgtcc tgggacgcga
     6541 caaaagatct taattttggc attcgctatc gttacgactg gaaagcttac cgacaacaag
     6601 acttatccgg tgatatgtct cgtgataacg ttcatcgttg ggatggatat gtcacttacc
     6661 atattaatag tgatttcacc ttcgcatggc aaacgacgct atacagcaaa cagaatgatt
     6721 atcgctatgc aaaccacaag aaatgggaga cggaaaatgc ctttgttcta caataccata
     6781 tgacgcccga tattacgcca tacatagaat atgactacct tgaccgccag ggtgtttaca
     6841 acggcagaga taatttatcg gaaaacagtt atcgcattgg tgtgtcattt aaactgtagt
     6901 agacaggaga cagtcacaat gaataaaact ataatggcgc ttgctatcat gatggcttca
     6961 tttgccgcaa acgcgtctgt attaccggaa actcctgtgc catttaaaag tggtaccgga
     7021 gcaattgata acgacactgt ctacattggt ttaggtagcg caggtacagc atggtacaag
     7081 ctagatacac aggccaaaga taaaaaatgg actgcgttag ctgcattccc tggtggaccg
     7141 agagagcaag caacctctgc atttattgat ggcaatctgt atgtgtttgg cggcattggc
     7201 aaaaacagcg agggattgac tcaggtattt aatgacgtac acaaatacaa ccccaaaacc
     7261 aatagctggg ttaaattgat gtcgcacgcg ccgatgggca tggcgggtca tgtgactttt
     7321 gtacacaacg gcaaggctta tgttactggc ggtgttaacc agaatatctt caatggctat
     7381 tttgaagatc tcaacgaggc tggaaaagat tcaaccgcta tagataaaat caacgcccac
     7441 tattttgaca aaaaagcaga agattatttc ttcaataagt ttctgttgtc ttttgatccc
     7501 tcaacacagc aatggagtta cgctggcgaa tcgccctggt acggaacggc tggtgcggcg
     7561 gttgtgaata aaggtgataa aacctggctt attaatggcg aagccaaacc aggattgcga
     7621 acggatgccg tatttgaact tgatttcacc ggtaataatt taaaatggaa taagcttgat
     7681 cccgtctcat caccagatgg cgtcgctggc ggttttgcgg ggataagcaa tgattctctt
     7741 atatttgctg gaggggccgg attcaaaggt tcacgagaaa attaccagaa cggtaagaac
     7801 tatgcgcatg aaggcctgaa aaaatcatat agcactgata ttcatctttg gcataacggg
     7861 aaatgggata aatcgggtga attatcgcaa ggtcgggcct acggagtatc attgccctgg
     7921 aataatagtt tattaattat tggcggtgaa actgcaggcg gcaaagcggt gacggattca
     7981 gttttaattt ctgtgaagga taataaagtc acagtacaaa actaacgctt cagggccccg
     8041 gtaaaggggc cctgctatca atttgtcatt tgtattacgc gaaaattata tgaacgcaat
     8101 aatatcaccc gattattact atgttcttac cgttgctggt cagtctaatg ccatggcgta
     8161 tggcgaagga ctgccattac cggacaggga agatgcgcct cattccagaa ttaaacaatt
     8221 agcgagattt gcgcatacgc atcccggagg cccgccatgt cactttaacg acattattcc
     8281 actgactcac tgcccacacg atgttcagga tatgcagggt tatcaccatc ctctggcaac
     8341 gaatcatcaa acacagtacg gcaccgttgg ccaggcactg catattgcac ggaaattact
     8401 gcccttcatt cctgataatg cagggattct tatcgttccg tgttgccgtg gcggatcggc
     8461 ttttaccgcg ggcagcgaag ggacatattc agaacggcac ggagccagcc atgatgcttg
     8521 tcgttgggga acggatactc cgctgtacca ggatttagtc agcagaacgc gagccgcact
     8581 ggcaaaaaat ccgcagaaca aattcctcgg cgtatgctgg atgcaaggcg aatttgactt
     8641 aatgaccagt gactacgcgt cacaccctca acactttaat catatggttg aagcctttcg
     8701 tagggatcta aaacaatacc attctcagct taataatatt actgacgcgc cgtggttttg
     8761 cggcgatacc acctggtact ggaaagaaaa tttcccgcat tcgtatgaag ctatttatgg
     8821 caattatcaa aataatgttt tagccaatat tattttcgtc gacttccagc aacaaggtga
     8881 aagaggactg acgaacgcgc ctgatgaaga cccggacgat ttaagcacgg gatattacgg
     8941 ttcagcgtac cggtcaccgg agaactggac gacggcactg cgaagcagtc atttcagcac
     9001 ggcagcccgt cgggggatta tttctgacag atttgtagaa gcaattttgc agttttggcg
     9061 cgaaaggtga gcggggagtt tattcatggg ggatgtcctt gttcgttctc agagtagaca
     9121 acaaaaaaca aatgagatca aggtaatatc tcttcacttg cgggctcgaa cagcaggcaa
     9181 ttgccaatat aataatctgg cacaagggtg aaaaacgcgc accgcataaa ccgttgttat
     9241 tgcttacaga ttagccggat gccttaatag acatccgcgc cttttcgatt atggctcgga
     9301 aatctacgaa catctacaca gtttactgga acgttttggg ccacagcgtt cgcagcaccg
     9361 tcctgatatg ccgttctggc gattataagt cgacggattc tggcaaatag acgatgatga
     9421 tatgcttaat gatgttcaaa gaaattaggc cattattata ttattgcttg cattttcttt
     9481 cattcctaaa agcacccgcc attaatccca ggcgcattat
//
LOCUS       DAJTSA010000137         9493 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000137 DAJTSA010000000
VERSION     DAJTSA010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.137"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..190)
                     /locus_tag="OO369_003639"
     CDS             complement(<1..190)
                     /locus_tag="OO369_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HCR5983767.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            710..1405
                     /gene="minC"
                     /locus_tag="OO369_003640"
     CDS             710..1405
                     /gene="minC"
                     /locus_tag="OO369_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR5983768.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1429..2241
                     /gene="minD"
                     /locus_tag="OO369_003641"
     CDS             1429..2241
                     /gene="minD"
                     /locus_tag="OO369_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR5983769.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2245..2511
                     /gene="minE"
                     /locus_tag="OO369_003642"
     CDS             2245..2511
                     /gene="minE"
                     /locus_tag="OO369_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR5983770.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2883..3227)
                     /locus_tag="OO369_003643"
                     /pseudo
     CDS             complement(2883..3227)
                     /locus_tag="OO369_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3262..3381)
                     /locus_tag="OO369_003644"
     CDS             complement(3262..3381)
                     /locus_tag="OO369_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR5983771.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3342..3527)
                     /locus_tag="OO369_003645"
     CDS             complement(3342..3527)
                     /locus_tag="OO369_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983772.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3628..3801
                     /locus_tag="OO369_003646"
     CDS             3628..3801
                     /locus_tag="OO369_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983773.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3803..4147
                     /locus_tag="OO369_003647"
     CDS             3803..4147
                     /locus_tag="OO369_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR5983774.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4151..4486
                     /locus_tag="OO369_003648"
     CDS             4151..4486
                     /locus_tag="OO369_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR5983775.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4543..6864)
                     /locus_tag="OO369_003649"
     CDS             complement(4543..6864)
                     /locus_tag="OO369_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5983776.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6981..7190)
                     /locus_tag="OO369_003650"
     CDS             complement(6981..7190)
                     /locus_tag="OO369_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983777.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7941..9464)
                     /locus_tag="OO369_003651"
     CDS             complement(7941..9464)
                     /locus_tag="OO369_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5983778.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         2657 a         2206 c         2081 g         2549 t
ORIGIN      
        1 cggctttttc gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac
       61 aaatatacgg gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc
      121 cagacgccat tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca
      181 tcatattcat agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc
      241 aatccgtaat caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac
      301 gtcttctgcg gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct
      361 gttctttaat gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata
      421 cacttgcctc aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg
      481 gaaaaaggaa gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag
      541 ggtatttttt aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata
      601 aatgggaggg tgacttgcct caatataatc cagactataa catgccttat agtcttcgga
      661 acatcatcgc gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac
      721 tccaatcgag cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga
      781 acctaaggtt atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa
      841 acatgccccc gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat
      901 gcataaggcg gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc
      961 gcaacttaaa gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa
     1021 agcgccacgt ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa
     1081 aacgcgttta atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga
     1141 tctgattgtt acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca
     1201 tgtctatggc atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca
     1261 aatattttgt acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag
     1321 tgatcaaatc ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc
     1381 tttgaccgtt caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt
     1441 attgttgtta cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc
     1501 actggtttgg cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt
     1561 aatctcgacc tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt
     1621 cagggcgatg caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat
     1681 attcttccgg catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa
     1741 gttcttgatg atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg
     1801 attgaaaccg gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac
     1861 ccggaagtct cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca
     1921 cgccgcgcag aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac
     1981 ccaggccgcg taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc
     2041 atcaaactcg tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt
     2101 gaaccggtca ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa
     2161 cgtctgttgg gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc
     2221 aaacgcttgt tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag
     2281 aacacagcca acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc
     2341 gatgcagaac cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa
     2401 tacgtacaaa ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt
     2461 tctattcttg agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt
     2521 aaaaacgcat ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat
     2581 ccacgttgtg gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc
     2641 cccctttaca ccttaaggct gttcccacgc acagaacaaa atgtttatgc ctggttgagt
     2701 aaataaccat gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt
     2761 cattgttctc ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga
     2821 attttctctt tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat
     2881 ggtcagaacg accagttcac accagccacc gcgttccacg gggactccac accggcacca
     2941 tggctatacc ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct
     3001 gcctggtata ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact
     3061 ttcacccgat tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt
     3121 tccataccgt tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata
     3181 ctgcgggtat cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt
     3241 gacctaagct ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag
     3301 agacagcgca ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata
     3361 tcccgcataa gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa
     3421 atcactcttt tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa
     3481 aataaaatga ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac
     3541 aataatattg ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca
     3601 cttcttgaat aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt
     3661 cacgtaaaaa acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat
     3721 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
     3781 tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc
     3841 attgttctgc tccacgccag cgatggcgga tatgaaccgc accacgaaag gtgcattgct
     3901 aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
     3961 tgttggcgct ggtgttagtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa
     4021 aggtgcaaaa gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
     4081 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
     4141 gaaataagga atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc
     4201 aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac
     4261 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
     4321 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
     4381 gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt
     4441 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac
     4501 cgcgtcgatg aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag
     4561 gcccaccatc actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc
     4621 ccagacgctg gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt
     4681 actcacccca tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt
     4741 atggatccag ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt
     4801 ctcctgagta cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc
     4861 attctcaata cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat
     4921 agcctgtgcc tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt
     4981 attcagggca aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga
     5041 ttcataggct aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga
     5101 gtcaatgtat gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc
     5161 gctatagcct ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt
     5221 gtagtcgcgg gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc
     5281 accggttaac agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg
     5341 tccgttgctg tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga
     5401 acgctcctgt tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg
     5461 cataacaaaa agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg
     5521 caggacgggg gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc
     5581 gctcgttgcc tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg
     5641 ggttaatgaa tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt
     5701 tgattccccc tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc
     5761 tgcattggta actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc
     5821 cgtagctgaa ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac
     5881 gtaattttca gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat
     5941 atagaccgta ctattatcaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca
     6001 ggtactatta tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc
     6061 tgaacccgtt atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat
     6121 aaaaacatct gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatc
     6181 tttatggtag aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt
     6241 tgcttcctga ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc
     6301 agatgtattt ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg
     6361 acccgttgta taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc
     6421 atgagcattg tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt
     6481 ataatcaagc gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc
     6541 tgttccgtgg gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc
     6601 attacttcct gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct
     6661 tccgggagat gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt
     6721 atcatctccg tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt
     6781 gtcagtattc aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt
     6841 gacaatcgtg tttttaccct gcaagataac agcaccgcca aattcactta cgattgcgct
     6901 agctgttaat gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac
     6961 aatgacgcca ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt
     7021 ccattacccg ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt
     7081 gccacagcat gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc
     7141 gccagagcaa taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta
     7201 tccattcatt ggattaacaa acattcctgg tcaaataacc tatgagctca gattaccaaa
     7261 acggagggta aagacaaaaa caaaagcaca aaaaatttaa atagataaac agcgacaata
     7321 tataaatgat aaataagtcg gcattatcat ttttcacctt attttcatga cattgatcac
     7381 tttggggtga ctattttgtt taacaaataa ctcttcgaca tcaaaattat taatagttgc
     7441 catggtaatt atttcggtga tatataaaaa caactgtgat aacaccaagg ttgacaacta
     7501 tttgcgcaat aattcctttc cttcaatgat ccaggcgttc actgccagca acccattcag
     7561 ccgcatactg acagtgagaa actgaaagat tattgctgat ccctgtggct aacgagtctt
     7621 tctcttaaat cgattaacca gattgatcgc cagccaaaca agggcattaa gcaaatttat
     7681 cgtcacaagc acttctccca gcccggtact gggttttgca ataaggatga ccaggagggt
     7741 gatcagcata aatgcaatgg aaaagatgat atagagagtg aaataatcca gattattact
     7801 attattcatg ctactaatgg caatcctgtc aaaactttca aacaattata gcaaacaaaa
     7861 taatgaacaa tagtaacaag aggcaacgag ctaattagct gtctataatt acaccgcact
     7921 atggccttac tgatcaattt tcactcaacc acaaccttca ccttcggttt agaaaggata
     7981 attttgacca ggtcgatgta tgtaacaggt ttatagaaat aataaccctg ctgaaatgtg
     8041 atattatttt ggttcagata gccaagttgt tctttcgttt cgacaccttc agcgacgata
     8101 ctgatcgaaa gtttacgcgc cagatccaat acacaatcca gaattcgggt tgattctggg
     8161 tctgcgttta cgcggccaac gaaactgtga tcaattttga tataatcaat atgcaggtca
     8221 tgaagataag aaagccctga gtagccagta ccgaaatcat ccagcgcgat gacaaaacca
     8281 ttttcatgca gaatgttcaa ccgctgaacc agactttcat caacattcaa tggctcacgc
     8341 tcggtgactt ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa
     8401 cactcgtcga caaacgtcgg cgaaataata tgcgaagcgc taaaattaat tccaatatga
     8461 aaaccttccg gcagtttact cgcgatagcg ttcatctgtc tggcaacctg attcatcagg
     8521 ctttgtgtaa gcggaacgat taatccggat ttttcagcaa gtggaataaa tgccgcgggt
     8581 gaaatatatc caccgtgagg ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc
     8641 ccttcccgac catttaccac aggttggtaa aaagggacga tctccccatt atttattgcc
     8701 cgtcgcagga tctcttcagg cgttgcgctt ttattgaaat atctatcaag caggaaggca
     8761 gcggcgctgg cgattaacag aataaatatt aaaatcccaa agcctttatc gataagtctg
     8821 tagaagctaa agagtggcgg cgggttaaag ataatagtga aatgatattt tgtggagtga
     8881 accttctcgg tatagggata atgtccatta agcgtcatca catcagccgt tggtccaata
     8941 atgtcatccg ccacgcgtaa tacatacctt acccctttca agggtacatt aagcgccccg
     9001 cgaatatgtt gatcacttat cgtaaccaaa atgcgcgtat ctgcaaattg gctctgatag
     9061 agcaggatag gtaatctatt aacggtgtcg attgccggag ccaacagtaa attactgtcc
     9121 gggacaacag gaatacgaga cagcaggacc cgattcccag gcagggatgt acaccagact
     9181 attccctgtt tgagaatgat gattgtgcgc aggtgaggtt tcagagctgc ttcagtgcca
     9241 agctgatatt gctcctcgag gtcgcattcc ttcccggcaa tgttaaccgc tgtttgagtc
     9301 gcctgtgacg cttcttcaag tatatgattg atattgttgg cggcatatct tgctcccgcc
     9361 aggtactctg cttttgcgga ataccagagc tggatgttca aaatagcgac accggtaata
     9421 aacaagcata tcgcaacgag gatgggtatg agtgtattgc gcatttgctt gtcccggcca
     9481 aagggtttaa tac
//
LOCUS       DAJTSA010000138         9333 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000138 DAJTSA010000000
VERSION     DAJTSA010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.138"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..256
                     /gene="mliC"
                     /locus_tag="OO369_003652"
     CDS             <1..256
                     /gene="mliC"
                     /locus_tag="OO369_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR5983779.1"
                     /translation="ERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            315..971
                     /gene="pdxH"
                     /locus_tag="OO369_003653"
     CDS             315..971
                     /gene="pdxH"
                     /locus_tag="OO369_003653"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR5983780.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1100..2374
                     /gene="tyrS"
                     /locus_tag="OO369_003654"
     CDS             1100..2374
                     /gene="tyrS"
                     /locus_tag="OO369_003654"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR5983781.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2433..3296
                     /gene="pdxY"
                     /locus_tag="OO369_003655"
     CDS             2433..3296
                     /gene="pdxY"
                     /locus_tag="OO369_003655"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR5983782.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="OO369_003656"
     CDS             complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="OO369_003656"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR5983783.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="OO369_003657"
     CDS             complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="OO369_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR5983784.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6164..6799)
                     /gene="nth"
                     /locus_tag="OO369_003658"
     CDS             complement(6164..6799)
                     /gene="nth"
                     /locus_tag="OO369_003658"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR5983785.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="OO369_003659"
     CDS             complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="OO369_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR5983786.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="OO369_003660"
     CDS             complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="OO369_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR5983787.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="OO369_003661"
     CDS             complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="OO369_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR5983788.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(9181..>9333)
                     /locus_tag="OO369_003662"
     CDS             complement(9181..>9333)
                     /locus_tag="OO369_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983789.1"
                     /translation="IARAKARKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAAQQ
                     KVVNED"
BASE COUNT         2388 a         2337 c         2371 g         2237 t
ORIGIN      
        1 tgagcgaatg caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa
       61 actgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa
      121 acagggcctt tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa
      181 aggtgaggaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca
      241 aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat
      301 ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg
      361 aatacaccaa aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg
      421 aacgttggct ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg
      481 ctaccgtgga tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg
      541 aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata
      601 atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg
      661 gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg
      721 atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc
      781 ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga
      841 gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc
      901 atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc
      961 ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac
     1021 tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt
     1081 tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg
     1141 gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat
     1201 cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc
     1261 attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg
     1321 cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga
     1381 agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt
     1441 cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa
     1501 tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa
     1561 agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc
     1621 ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct
     1681 gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg
     1741 tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac
     1801 caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta
     1861 caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt
     1921 cttcaccttt atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg
     1981 taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga
     2041 agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc
     2101 gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga
     2161 aaagggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca
     2221 ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc
     2281 tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg
     2341 caaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag
     2401 aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt
     2461 ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg
     2521 gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat
     2581 ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca
     2641 ttgataaatt acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg
     2701 gtgaacatat cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt
     2761 tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag
     2821 agtttcatgt gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc
     2881 tggaaatact ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg
     2941 aactcattga gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca
     3001 gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga agtctggcat atcagccgtc
     3061 cactggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac
     3121 tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg
     3181 cagtctacga aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg
     3241 ctgctcagga tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata
     3301 tctcatttcg gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga
     3361 cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc
     3421 taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc
     3481 atcagcaatt gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac
     3541 atattgcagc ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc
     3601 tgggcgaaac agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc
     3661 gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct
     3721 gtcggcaaga tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag
     3781 caatgcaggc acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg
     3841 ttttttcatt aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat
     3901 atgggaagcg agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg
     3961 tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt
     4021 tagcggcttg taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc
     4081 tgcagcgtca tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc
     4141 aataacggca gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat
     4201 cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag
     4261 gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg
     4321 aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg
     4381 caggccatag cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt
     4441 cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt
     4501 ggttggcatc ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc
     4561 gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag
     4621 aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta
     4681 cagcacgaag aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg
     4741 cgcagcacct ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac
     4801 aacgcccagc gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat
     4861 cagcgccaca acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc
     4921 tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat
     4981 cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca
     5041 cggggtggct atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa
     5101 tgcaccgtcc agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt
     5161 agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc
     5221 gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat
     5281 aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc
     5341 ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag
     5401 ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca
     5461 taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac
     5521 gctttcagtt ggtttttggt ttgcagtgga cacttttacc tcttttttta catcccatat
     5581 taacgggggt gctttctcgc gttatgtcta aaaaacatac gcgcaattgt tatagtggga
     5641 ggggaaacgg caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc
     5701 tgttacatat aactgaggcg gggaattcaa ccattccggg aaatgttatc cagcgttaaa
     5761 tttttcaaaa aacgtaacgg cagatttttt cactgcattg agagggggat gatggcttgg
     5821 cggcgatatg ttctgctatt tagcgttttt cctagtgaga tagtccagtt tctgaaaaat
     5881 agccagtgta atgttttgca ggtcaatatt tgttgcgtgt gtaaatttaa atcattttaa
     5941 cctgattctt gcaatgtttt tacattgcca cagcatcgtg atcttgatca cgaatgaata
     6001 gaaaatttcg ctattaaaaa aacaattaac ctgcattttt ggttatccgc aaacggatta
     6061 cctgatgata acgctaagca atggcatcat tgacgttaaa aagatgtttt aaaagaggat
     6121 aaagaaaggt tatcaatggg gtaatcggtg ttaccccttt tcttcagatg tcaactttct
     6181 ctttgtattc acaaagatct tcaataatac aagagccaca gcggggcttg cgggcaatgc
     6241 aggtataacg cccgtgcagg atcaaccaat ggtggcagtc gactttaaac tctgccggaa
     6301 ccactttcag tagcttttct tctacctgtt cgacgttttt ccccggcgca aattgagtac
     6361 gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg aatgcagtgt
     6421 ttaatacgac gttggctgtt ttacgaccta cgccgggcag ggcttcaagc gcagcacgat
     6481 cttccggaac ctcgccatta tgttgttcca gtaatatacg gcaggttttg atgatatttt
     6541 ctgctttgct gttataaagg ccaatcgttt tgatataggt tttcacccct tcaacgccta
     6601 gttcaagcat cgctgcaggc gtattcgcca ccgggtagag tttcgccgtc gccttattaa
     6661 cactgatatc ggtcgcctga gcggaaagca gtacggcaat cagcaattca aaaggcgaac
     6721 tgaaattaag ctcggtggtg ggatgaggat tgttctcacg caggcgagtg aggatctcca
     6781 gacgttttgc tttgttcatc agacattccc tgtttcaccg tttggcaatg cacgttcggc
     6841 agctgcttca gcacggcgct ttttcattct ttcatcaatc aggtattttc ctgccagcat
     6901 cagtcccaag ccaataaatg cacctggtgg cagcatcgcc agcaggaaag gggagtcggt
     6961 gtggaaaatc tccacgcgta atacttttgc ccagctacct aacagcgcat ctgcaccgtc
     7021 aaacaacgtg ccattgccga taatttcgcg tagtgaaccc agcacgaaca tggcgcaggt
     7081 tgcgcccgta ccaattgaaa agccgtccag tgccgaaagc gccggacctt ttttggcggc
     7141 gaaggcttca gcgcggccca caacgataca gttagtgaca atcagcggaa taaaaatccc
     7201 taatgattga tacaggccaa aggcgtaggc gttgatcagc atctgtacag cgctgaccac
     7261 cgaggcgatg atcatcacgt aaatgggaat gcggatctcg gctggcgtcc agtgacgcag
     7321 cgtcgaaatg gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc ctaaacccag
     7381 agcgttagtg gcagtggatg tgaccgccaa tagcggacaa aggccgagca gttggaccag
     7441 tgcagagttg tttttccaca acccctgaac aataacgtct ttaatttcgc tcacgcttta
     7501 ttctccacag gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt acaatccggc
     7561 gcgttttacc gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga actggtcgaa
     7621 atcaccacca tctttcttca ccgcccagtg cgcatcatcc gcaccactga ttttttttcc
     7681 cgcaaaatgg gtgagccagt cagaaaggcg caattcgatt ttatcgccaa gccctggcgt
     7741 ttcgtggtgc tctgtcacgc gcgtgccaag taccgtgcca ttaaaatcgg ctcccaccag
     7801 cagctgaatc gcaccggaat agccatctgg cgcggttgct tccagaacgg cggctaccgg
     7861 tttgtcatcc tgtttggcga tgtaaacccg atgctcacct ttacctaact ctggcgcagt
     7921 taccagatag caactctgtg cgagcgcatt gttatagcgt tcggctggca gcacctgatc
     7981 aaataacgtc ttttgttgca gactggcctg ttcagcaatt gtcgttttgg tcatctggtt
     8041 gatggccgca gttaaccctg tagaacccgc tgcaaatagt gccagtgtaa tgccgtgttt
     8101 tcggatagtt ttcagcatgg tttacccttt gcgatggccg tagacgcgcg gacgcgtgta
     8161 gtaatcgatc agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca cgccgtcagg
     8221 atagccgccg aaactgcgga tcaaccagac taataagccc gccagcgcgc cgaacatcag
     8281 ccgaccacga ttggtcgtag aagcagtaac tgggtcagtc aaaataaaga atgcgccgag
     8341 catagtcgca ccagacagca gatgaatttg cggtgctgcc agtgtttctg gtgagaacaa
     8401 ccagcccaac gttgcgcata acgccagcgt tactaagaag ctgaggggaa tatgccagcg
     8461 aatcgctttc tgccatagca accacacgcc accagccagc caggcgagat ttacccattg
     8521 ccaaccagcg cccgccagaa taccgctgta gatcggatat tgcataatct gttcaaccga
     8581 atgacctgcg cggacagagg ttttgaatgt atccagcggt gtcgcctgac taatgccatc
     8641 aatacctaag cgtagcgtat tcatatcacc accactggcg gtatgaccgc tgaaaataac
     8701 ctgtatggcg tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg gtaaccagct
     8761 ggtcatctgc accgggaagg agatcagtaa gaccacataa ccaatcattg ccggattaaa
     8821 cgggttttgc cccagaccgc catacaactg tttagcgatg atcaccgcaa acaccgtacc
     8881 cagtacgacc atccaccatg gcgctagggg gggaatactt accgccagca ataagcctgt
     8941 cagcaatgct gagttatctt tcaacgttgc ggctaccgac tgcttgcgta gtttgagtac
     9001 gagagcttcg gctaacagag cactgaccga cgccaacagg atctgaacga gagtacccca
     9061 accaaaaaac cgcagttgcg ctgcgattcc tggcacggct gcgagcaaca ccaacagcat
     9121 aatgcgcgat gtctggcgct ggttatgggt ataaggggag ctagctattc tgaataccat
     9181 ttagtcctcg tttacaacct tttgctgggc ggcttttttg gcctgaacgc gggcaatagc
     9241 cgcggcaact gcggctttgc gcggatcgac ctgttcttct ggttccgcat tagcctgttg
     9301 ctgttccagc ttgcgtgctt tggcgcgggc aat
//
LOCUS       DAJTSA010000139         9294 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000139 DAJTSA010000000
VERSION     DAJTSA010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.139"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            39..1436
                     /gene="ccp"
                     /locus_tag="OO369_003663"
     CDS             39..1436
                     /gene="ccp"
                     /locus_tag="OO369_003663"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR5983790.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1647..3047
                     /locus_tag="OO369_003664"
     CDS             1647..3047
                     /locus_tag="OO369_003664"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5983791.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKQGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            3417..4241
                     /gene="gadX"
                     /locus_tag="OO369_003665"
     CDS             3417..4241
                     /gene="gadX"
                     /locus_tag="OO369_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR5983792.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            4609..5337
                     /gene="gadW"
                     /locus_tag="OO369_003666"
     CDS             4609..5337
                     /gene="gadW"
                     /locus_tag="OO369_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR5983793.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(5700..8813)
                     /gene="mdtF"
                     /locus_tag="OO369_003667"
                     /pseudo
     CDS             complement(5700..8813)
                     /gene="mdtF"
                     /locus_tag="OO369_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(8838..9095)
                     /locus_tag="OO369_003668"
                     /pseudo
     CDS             complement(8838..9095)
                     /locus_tag="OO369_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         2431 a         2163 c         2279 g         2421 t
ORIGIN      
        1 tctgggtcac aataactcac caccgtgagg gaagaataat gaaaatggtc tcacgtatta
       61 ccgcgatcgg cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt
      121 accacgataa taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata
      181 ataaggtttt aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag
      241 aattacccgc ctattattat attcctggcg cgaaacagtt gatggattac gacattaagc
      301 ttggatataa atcttttaac ctcgaggccg tgcgtgcggc actgctggct gataaacccg
      361 tttcgcaaag cgatctgaat aagattgaat gggtgatgca gtatgaaact atgccaccaa
      421 cgcgttatac cgcgctacac tgggcgggta aggtgagtga tgaagagcgg gcggaaatac
      481 tggcctggat tgcaaaacag cgcgcggaat attacgccag caatgatact gctccggagc
      541 atcgcaatga accggtgcag cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg
      601 cgttgggctt tgcgctgtat cacgatcccc gtttatcggc tgatagcacc atttcatgcg
      661 ctcattgcca tacgttgaat gcggggggcg tcgatggcag aaaaacatcg attggtgttg
      721 gtggcgcagt tgggccgatt aatgcgccga cggtatttaa ctcagtattt aacgttgagc
      781 agttctggga tggtcgtgcg gcaacattgc aggatcaggc aggtggaccg ccgttgaacc
      841 cgattgaaat ggcgtcgaaa tcctgggacg aaattattgc taagctggaa aaagatccgc
      901 agcttaaagc gcagttcctc gaagtttatc cgcaaggttt cagtggcgaa aatattactg
      961 atgccattgc tgaatttgag aaaacattaa ttacgccgga ttccccattt gataaatggt
     1021 tgcgtggaga tgaaaatgcg ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag
     1081 ataataaatg tgcaacttgt catggtggta ttattctcgg cggacgttcc tttgaaccgt
     1141 tggggctgaa aaaagacttt aactttgggg aaattacggc ggcggatatt ggtcgtatga
     1201 atgtgactaa agaagagcgt gataaattgc gtcagaaagt acccggttta cgtaacgttg
     1261 ctttaacggc accgtacttc catcgcggtg acgtgccgac gctggacggg gcggtgaaac
     1321 tgatgctgcg ctatcaggta ggcaaagagc tgccgcagga ggatgtggat gatatcgtag
     1381 ctttcctgca cagtctgaac ggggtgtaca cgccgtatat gcaggataaa caataattaa
     1441 tttgatcgcc cgaacaggaa tgtttgggcg atttttatta cgataataaa gtctgttttt
     1501 aatattatca tgttaaatgt ttatattata aaaagtcgtt tttctgctta ggattttgtt
     1561 atttaaatta agcctgtaat gccttgcttc cattgcggat aaatcctact tttttattgc
     1621 cttcaaataa atttaaggag ttcgaaatgg accagaagct gttaacggat ttccgctcag
     1681 aactactcga ttcacgtttt ggcgcaaagg ccatttctac tatcgcggag tcaaaacgat
     1741 ttccgctgca cgaaatgcgc gatgatgtcg cattccagat tatcaatgat gaattatatc
     1801 ttgatggcaa cgctcgtcag aacctagcca ctttctgcca gacctgggac gacgaaaacg
     1861 tccataaatt gatggatttg tcgatcaata aaaactggat cgacaaagaa gaatatccgc
     1921 aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg catgcgcctg
     1981 cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag gcctgtatgc
     2041 tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca ggcaaaccaa
     2101 cggataaacc aaacctagtg tgcggtccgg tacaaatctg ctggcataaa ttcgcccgct
     2161 actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt atggacccga
     2221 aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga
     2281 cctacaccgg taactatgag ttcccacaac cgctgcacga tgcgctggat aaattccagg
     2341 ccgacaccgg tatcgacatc gacatgcaca tcgacgctgc cagcggtggc ttcctggcac
     2401 cgttcgtcgc cccggatatc gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg
     2461 cttcaggcca taaattcggt ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg
     2521 aagaagcgct gccgcaggaa ctggtgttca acgttgacta cctgggtggt caaattggta
     2581 cttttgccat caacttctcc cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc
     2641 tgcgcctcgg tcgtgaaggc tataccaaag tacagaacgc ctcttaccag gttgccgctt
     2701 atctggcgga tgaaatcgcc aaacaggggc cgtatgagtt catctgtacg ggtcgcccgg
     2761 acgaaggcat cccggcggtt tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc
     2821 tgtacgacct ctctgaacgt ctgcgtctgc gcggctggca ggttccggcc ttcactctcg
     2881 gcggtgaagc caccgacatc gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg
     2941 actttgctga actgttgctg gaagactaca aagcctccct gaaatatctc agcgatcacc
     3001 cgaaactgca gggtattgcc cagcagaaca gctttaaaca cacctgataa cataacgttg
     3061 taaaaaccga atgcccagcc tttaaaaaaa cagctgggca ttcggttgct tattaatcgc
     3121 aataatatat tggctgtcta ttcatcgtgt tgataagata tatgttatat gaatgtttat
     3181 taaatgtagc gattaccttt aatgtcacga aaagggatta tttgcttgct attaatttca
     3241 ctgcgttctt aaacacgttt ttgtattata aatccagtca tcctgcccgt agaacatcac
     3301 acattatcat cctgttctcc cgcttcgttt aaatttattt atcaatcaat ttgacttaag
     3361 agggcggcgt gctacattaa ttaacagtaa tatgtttatg taatattaag tcaactatgc
     3421 aatcattaca tgggaattgt ctaattgcgt acgcaagaca taaatatatt ctcaccatgg
     3481 ttaatggtga atatcgctat tttaatggcg gtgacctggt ttttgcggat gcaagccaaa
     3541 ttcgagtaga taagtgtgtt gaaaattttg tattagtatc aagggatacg ctttcattat
     3601 ttctgccgat gctcaaggag gaggcattaa atcttcatgc acataaaaaa atttcttcat
     3661 tactcgttca tcactgtagc agagatattc ctgtttttca ggaagttgcg caactatcgc
     3721 agaataagaa tcttcgctat gcagaaatgc tacgtaaaag agcattaatc tttgcgttgt
     3781 tatctgtttt tcttgaggat gagcacttta taccgctgct tttgaacgtt ttgcaaccga
     3841 acatgcgaac acgagtttgt acggttatca ataataatat cgcccatgag tggacactag
     3901 cccgaatcgc cagcgagctg ttgatgagtc caagcctgtt aaagaaaaaa ttgcgcgaag
     3961 aagagacatc atattcacag ttgcttactg agtgtagaat gcaacgtgct ttgcaactta
     4021 ttgttataca tggcttttca attaagcgag tcgcagtgtc ctgtggatat cacagcgtgt
     4081 cgtatttcat ttacgtcttt cgaaattatt atgggatgac gcccacagag tatcaggagc
     4141 gatcggcgca gggattgccg aaccgtgact cggcggcaag tattgttgcg caagggaatt
     4201 tttacggcac taaccgttct gcggaaggaa taagattata gagttttact cagacataaa
     4261 aaaaacccgg cataggggac cgggaagagg atagtctgcc gtctccagac taataaaccg
     4321 ttataacact ccctgttggc acgggaaact ttgtgctctc agtaagttaa atataacttt
     4381 tacaggaaat aagatcagcc atttttttat aaacataagc tatacgctgt gcgaaaatat
     4441 attcgtgctg catttactta ttatcaatta actgttatgc aaaactactt tgtggataaa
     4501 ttttggtcct accaaatctg gcagtttttg cgctaagaaa cagtctgtca tcatttcatt
     4561 agtatactga aattgaaata atcgcagtat gaaatataag ggataatcat gactcatgtc
     4621 tgctcggtga tcctcattcg tcgttcattc gatatttatc atgaacagca aaaaatatcg
     4681 ctgcataacg agagtatcct gctgctggag aaaaatttgg cagacgattt tgcgttttgt
     4741 tcaccggata cgcgacgact ggatatcgat gagctgacag tttgccatta cttacaaaat
     4801 attcgtcagc taccacgcaa tttagggtta catagcaaag accgtctgtt aattaaccag
     4861 tcacccccca tgccgctggt gacggcgatt tttgatagct tcaatgaatc cggggtaaat
     4921 tcaccgatac tgagcaatat gctctacctt tcctgtttat cgatgttttc tcataagaaa
     4981 gaactgatcc ccttactttt caatagcatc agcactgttt caggaaaagt tgaacgcctt
     5041 attagctttg atatcgccaa acgttggtat ctgcgcgata tcgcggaaag aatgtatacc
     5101 agcgagagtc tcatcaaaaa aaagttgcag gatgaaaata cctgtttcag taaaatatta
     5161 ctcgcttcca ggatgtcgat ggccagacga ttactcgagt tacgtcaaat tcctctgcat
     5221 actattgcgg aaaaatgtgg ctatagcagt acgtcgtact ttataaacac atttcgacaa
     5281 tattatggtg taacgccaca tcagtttgcg caacattcgc caggtacctt ttcctgacat
     5341 attttgcatt tgaatattgg tcaggatctc acacctgctt catgtgaaac tcttccctga
     5401 tgatttctgc cgggctaccg gctagttctc tttcgcagtt tgccaggcgg tataggtatt
     5461 tttaggagga aaagactcgg catgtttggg attattaagc tgacaattca tacgattacg
     5521 ggaatgtggg tcagtattgt gttgttcaaa ttgatgacga atggctggag tggtttctac
     5581 tttcagtgct gtgtactaag cctggtattc ctgacggtta gctggttgtt gagtggtgaa
     5641 tggttagcag gaaagagtaa ggctgaacct tcacgttcaa ccttactctc atttacacgt
     5701 tacgcttttt taaagcgggc aaagagatgt tccactacaa cgaaaaagac cggaacgaag
     5761 taaattgcca gcactgttgc ggcaaacatc ccgcccatca cgccggtacc taccgcgttt
     5821 tgcgcgccag aaccggcacc atgactgata accagcggca gcacgccgag aataaaggcc
     5881 agagaggtca tcaggattgg gcgtaaacgc atccgcgccg cttcgatgat tgcctctatc
     5941 ggcgttttcc cttctttctg catcatctca acggcaaatt cgacaatcag gatggcgttc
     6001 ttggcggaaa gcccgatggt ggtcagcaaa ccaacctgga agtagacgtc attacttaag
     6061 ccgcgcagat cggtggccag taatgcgcca acgacgccta acggaacaac caacatcacc
     6121 gagaacggaa ttgaccagct ctcatagagt gcggcgaggg cgaggaacac cacgaccagt
     6181 gaaatggcat acagtgcagg agcctggttt gaggataacg cttcctgata cgacagtccg
     6241 gtccatgagt agccgacgcc tgccggaagt ttagcgacca ggtctgccat aaatttcata
     6301 gcgtcaccgg tacttttccc ggccgccgct tcacctaaaa tctccattga cgggatgccg
     6361 ttgtagcgtt ccaatcgcgg tgaaccatag gtccattcgg tagacgagta ggcagaaagc
     6421 ggtgccatcg tgccagaggc gttgcgtaca taccattggt tgatgttatc cggcaacata
     6481 cggaacggcg tgcctgcctg gacatacact tttttcaccc gcccctggtt gaggaagtcg
     6541 ttcacgtagc tgctgccgaa ggcggtggaa attgtctggt tgatatcaga cagcgccaca
     6601 cccattgcct cagctttcgc ggcgttgacg ttcactttga acatcggcgt atcttccagg
     6661 ccgttcgggc gtaccccggt gacctggtca ggcgattgcg ctgccagtga taacagctcg
     6721 tttcgtgcct aggttagttt ttcgtgcccc aggttaccgt tgtccagcag ttccatatca
     6781 aaacctgacg cggtacccag ttcagccacc gcgggtaagt tgaacgggaa gacgacggct
     6841 ttattgatac tgcttaacgc aatcattgcc cgctgaatga tcgcggtaac cgagttttcc
     6901 tcaccgacac gttcagacca cggcttgaga ctgataaacg ccaggccgtt gttttgcccc
     6961 tggccgctga agccaaagcc gccaacggta aacaccgact gaacattatc tttctctttt
     7021 gtaagataat aatccgtcac ctgttgcagt actttcgtgg tgttaaccat ggtggcaccg
     7081 gaaggtaact gcgcggtggt cataaatacc ccctgatcct cttctggtaa gaaagaggtc
     7141 ggtgtacgca ggaacagcac cgccatcccg gcgcaaatca gcaggtagac caccatgtag
     7201 cgaccggtac aacgcaacag cgagcgggtg ctatcggtat agtgctgagt tgatttttca
     7261 aacagcgtgt tgaagcgtgc gaacagggcg ttaggtttgt gaccgccttc cggcgtggct
     7321 ttcagaatgg tggcgcacag ggcaggggtc aggctcattg ccacaaatac tgaaagccgc
     7381 atggaggaga tcagcgtgat ggagaactgg cggtagatct ccccggttgc accgctcata
     7441 aaggccatcg gcataaacac tgcagaaaga acaacggcaa tcccgaccag cgcacgttgg
     7501 atctgcccca tcgatttatg cgtcgcttcc ttcggcggta gcttatcttc cgcaatgaca
     7561 cgctcgacgt tctccaccac cacgatggcg tcatccacca gtaaccctat cgccagcacc
     7621 atcccgaaca tagtcaacgt gttgatggtg aaaccgaccg ccgacaagat cgcaaacgtc
     7681 ccgagaataa ccaccggtac ggcaattgtc gggatgatgg tggcacggaa attctgcaaa
     7741 aacagataaa tgaccaggaa gacgaggatg atagcctcaa ccagtgtttt gaaaacttcc
     7801 tgaatagaaa tttcgataaa cggcgtggtg tcgtaaggat aaaccgtctt cagacttgcg
     7861 gggaaatagg ctgataagcg gttcagctcc tctttgactg cccgcgaggt atccagggcg
     7921 tttgctccgg cagccagttt gatggcgatc ccggcaacag gtttgccgtt atagcgcgcc
     7981 acggtggaat aatcttccgc cccaagttcg acgcgggcga catcacgcag cagcacttgc
     8041 gaaccatctt gctgaacttt caacaggatt ttgccaaatt cttccggcgt ttgcagacgc
     8101 gtctgcacaa tgatcgaggc gtttagttgc tggtctgccg cctgtggcat gccacccagt
     8161 tgaccaccag aaatctggtt gttttgcacc ttaatctggg aaataacatc ggaaggtacc
     8221 aggttatatt tattgagttt ttgcgggtcc agccagatac gcatggcgta ctcggaacca
     8281 aagagctgta cgctaccaac gcccgcggta cggcttagcg ggtctttgat attagacgct
     8341 acatagtccg cgatatcgta ctggttgagg ctgccgttat cagaaataaa cgccgctacc
     8401 atcaggatat tactgctcga cttatcgacg ctaatcccct gttgctgcac cgcttcaggt
     8461 aatgaaggca tagcgagttg cagtttattt tgcacctgaa cctgtgcgat atcaggagat
     8521 gccccagtct cgaaggtcag agtgatagag gcattacccg ccgcatcact ggttgaagac
     8581 atgtacatca ggccatcaag cccattcata ttttgctcaa tcacctgagt gaccgagtct
     8641 tctaccgttt gcgcatcggc acccggatag gtagcgctga cggtaatggt cggtggcgca
     8701 atctgcggat actgcgcaac cggtaagttc atgatcgcca gaccacctgc aagcatcata
     8761 ataatggcaa gtacccaggc aaaaaccggg cgatcaataa aatagttagc catgaaagtc
     8821 cccttaagcc tgcaacgtta ttgtttcgat tcggtgctgg cgttttcctg gctggaggaa
     8881 attgctcgtg ctttgatacc cggacgaatg cgttgcaaac cggaaacgat cacccgatcg
     8941 ccagcctgca agccagaggt gacaacccac tggtcgccga tggctttgct ggcttcaatt
     9001 tcgcgtagct gcacgacatc gtctttatcc agaatgagcg ccgttgcttt accctgggcg
     9061 ttgtgggtga cgccttcctg cggcaccagt aatataaagc gcgcaaaatt attcaagata
     9121 tttttgaaaa tcattcaaga taggctatcg cgtgtgatag caaaaacatt caagataaaa
     9181 atgcttgatt attaagcaat caggaatggg agtattaagt ttcgtgtttc cagtaaaacc
     9241 agagagtatt gaaatagcta tcacctaact ttatcaggcg atagctggta gatg
//
LOCUS       DAJTSA010000140         9242 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000140 DAJTSA010000000
VERSION     DAJTSA010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.140"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO369_003669"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO369_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR5983794.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO369_003670"
     CDS             1828..3147
                     /locus_tag="OO369_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR5983795.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3445..3486
                     /locus_tag="OO369_003671"
     CDS             3445..3486
                     /locus_tag="OO369_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983796.1"
                     /translation="MVQLVDLARCVSF"
     gene            3524..4669
                     /gene="dosC"
                     /locus_tag="OO369_003672"
     CDS             3524..4669
                     /gene="dosC"
                     /locus_tag="OO369_003672"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR5983797.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="OO369_003673"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="OO369_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5801
                     /gene="ddpX"
                     /locus_tag="OO369_003674"
                     /pseudo
     CDS             5469..5801
                     /gene="ddpX"
                     /locus_tag="OO369_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5815..6711
                     /locus_tag="OO369_003675"
     CDS             5815..6711
                     /locus_tag="OO369_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR5983798.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6708..7682
                     /locus_tag="OO369_003676"
     CDS             6708..7682
                     /locus_tag="OO369_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5983799.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="OO369_003677"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="OO369_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OO369_003678"
     CDS             complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OO369_003678"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR5983800.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2259 a         2257 c         2317 g         2409 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 gaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctggcgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     5701 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     5761 acagccgtgg cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg
     5821 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5881 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5941 atgcgactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     6001 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     6061 cgcgatctgt ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg
     6121 gtggtcgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6181 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6241 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6301 attgctattg tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta
     6361 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
     6421 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
     6481 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctgggtgc tcaacaaccg
     6541 agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg
     6601 tattgcgcat ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt
     6661 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6721 ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc
     6781 tcaacaatgt gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg
     6841 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6901 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6961 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     7021 ttaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     7081 taagtcgtcg ggaagccaga gctaaagcga ttgcactgct ggaagagatg caaatcccgg
     7141 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7201 taatgattgc gccggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga
     7261 cggcgctgga cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg
     7321 ccagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
     7381 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta
     7441 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7501 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7561 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcacctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggcaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttgctgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gatctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgacctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
     8701 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
     8761 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
     8821 ccactttatc cagcgacaca tcggcggtgg tatcaattga tgttggcgtg aatcccgctt
     8881 cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt
     8941 cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt
     9001 tcagcacgcc gctctcggtg gatactgttc ccttcccgcg tttgatatcg ccttcccagt
     9061 gtgcctgacc tttcttatgg attgtcattg ttgctctcct gtgggcttaa aaaggaaatt
     9121 atagactacg tgagaaacag cacgaggata agcaggatat tccgaataaa atacggcgaa
     9181 aatattactt ctgcacttaa attaaattcc cacaatagat aaccgggaat atatatctca
     9241 ag
//
LOCUS       DAJTSA010000141         9191 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000141 DAJTSA010000000
VERSION     DAJTSA010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9191
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.141"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..904)
                     /locus_tag="OO369_003679"
     CDS             complement(<1..904)
                     /locus_tag="OO369_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCR5983801.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNV"
     gene            964..1242
                     /locus_tag="OO369_003680"
     CDS             964..1242
                     /locus_tag="OO369_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983802.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="OO369_003681"
     CDS             complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="OO369_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR5983803.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="OO369_003682"
     CDS             complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="OO369_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR5983804.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3396..3728
                     /gene="hdeA"
                     /locus_tag="OO369_003683"
     CDS             3396..3728
                     /gene="hdeA"
                     /locus_tag="OO369_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR5983805.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3844..4170
                     /gene="hdeB"
                     /locus_tag="OO369_003684"
     CDS             3844..4170
                     /gene="hdeB"
                     /locus_tag="OO369_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR5983806.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4234..4880
                     /locus_tag="OO369_003685"
                     /pseudo
     CDS             4234..4880
                     /locus_tag="OO369_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4922..5452)
                     /locus_tag="OO369_003686"
     CDS             complement(4922..5452)
                     /locus_tag="OO369_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5983807.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5608..6174)
                     /gene="slp"
                     /locus_tag="OO369_003687"
     CDS             complement(5608..6174)
                     /gene="slp"
                     /locus_tag="OO369_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR5983808.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6421..7644)
                     /locus_tag="OO369_003688"
     CDS             complement(6421..7644)
                     /locus_tag="OO369_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983809.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIEFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8225..>9191)
                     /locus_tag="OO369_003689"
     CDS             complement(8225..>9191)
                     /locus_tag="OO369_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5983810.1"
                     /translation="EMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGRG
                     YLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTFG
                     WKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKV
                     SCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIY
                     GFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALI
                     IGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2681 a         1827 c         1922 g         2761 t
ORIGIN      
        1 atacattctg gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt
       61 caccatttgg gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg
      121 tcgggtcgga gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga
      181 gctgtactgg cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca
      241 tgcgtaagaa atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta
      301 cggtaaccag cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt
      361 tcccgctgac gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct
      421 gttcaacagc cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg
      481 cggtgtcgta atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa
      541 aggtgatgcg ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt
      601 ttagctcggc ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt
      661 tatcgccttc gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg
      721 caacttcata aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccagggg
      781 agagtgtgac gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat
      841 cgcaggcggt gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt
      901 tcattttagt ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga
      961 gatatgaggg atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac
     1021 aggcacagtg atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca
     1081 gttcctgcca gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat
     1141 caagtcgttt tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc
     1201 cctcgtcatg ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat
     1261 acccagggtg acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt
     1321 ctgaatcttt aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt
     1381 atttttcaga atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt
     1441 cttatggggc aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg
     1501 gctaacaaaa taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac
     1561 aataattctc tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg
     1621 attgtgatag gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga
     1681 cagggaattg atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag
     1741 aaaagaatct ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa
     1801 catgtcatcc ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc
     1861 ctataagaac tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga
     1921 tttatccatc acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt
     1981 gtaaatagta aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata
     2041 accagtttgt gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc
     2101 gagtctcctt gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg
     2161 aaattacctg agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca
     2221 cggatattta tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt
     2281 taaccgccca tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta
     2341 aaacattaaa cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg
     2401 ccatggagtg aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat
     2461 tatttcaatg atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg
     2521 gatcatagtc acatcaagtg actatgatcc gggtgacaac cggggtaatt attgctgctt
     2581 aacgaacaaa ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac
     2641 cagtgtggaa accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat
     2701 gacgatatcc agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg
     2761 ataccaactc atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc
     2821 aatggcaaaa atgcccagct ccggtgcacg gatgaagaaa tagccgatca ataaataggc
     2881 gactgcgacg aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa
     2941 cccgacaata agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat
     3001 atcgccagag acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc
     3061 aataaactgg attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt
     3121 tgccttatct atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta
     3181 tcaatctatg ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg
     3241 cagtatctga tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt
     3301 tccatcaaca tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt
     3361 gacggctctt tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc
     3421 ttggtggtct gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg
     3481 ataacaaaaa accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct
     3541 tccagccaac tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg
     3601 cggttttaga tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc
     3661 aggataaaca agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag
     3721 atatgtaatt ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata
     3781 tcttctttaa aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac
     3841 aaaatgaata tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg
     3901 tcactggtga acgcacaatc tgcgttggca gccaatgaat ctgctaaaga tatgacctgc
     3961 caggaattta ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat
     4021 gaagaaacag tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa
     4081 attcctaaag tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa
     4141 aatcaagcat ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt
     4201 gagccatctt gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt
     4261 atactcgcgg caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga
     4321 gcagggttac gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg
     4381 aaatatggtt ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt
     4441 gctgctcagg tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac
     4501 caaaatattg taggtctgac gacggcagcg gatatctggg tgaccgccgc ataggtatgg
     4561 ttattggcag cggtatgtac gaactgggta tttatggttc agtgatgacc ttgttggtgc
     4621 tggaagtctt ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga
     4681 cattagtgaa cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa
     4741 ctgatttagt ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc
     4801 ttcatgccac aacgttgata gaagacctgt tgcgattatt aaaaggtatg gcaggggtga
     4861 aaggcgtttc aattagctaa tctgtgtggc agcgtagcca gactcaccgt aagcctgaaa
     4921 ttcacaccag ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga
     4981 ggatcatcat gatattgtgt ttatggcagt agaacgtttt tagcgatatt ttcagtttct
     5041 ccagaatatc ttcagttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc
     5101 gcgaaagtga cacattcgct tcaggcacag agcagtaact gccattgatg agtgcgacaa
     5161 aatcaatgat agtgatagtc cgcggaacaa aggtcaccgg tactttcccc agacagcgtt
     5221 taatattaaa gggggagaaa acaatgacat ggacaggttt taatcgttca atttgagtga
     5281 gaaaattaga atgaaataca ttattcatta atgtatcaat aatgacgaag gcattctgat
     5341 gcaacattac gtcgatctct tcttcgttct gtatatgaac gacattacct ttactcagaa
     5401 tgtttttcat cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact
     5461 cctggtgcgg acattaactt tataatcagt ttgccaaagg taattaacag gacagggctt
     5521 ataataataa tagttagctt actgtatatt aattttatgt aatacatgat gcagatgctg
     5581 agaaagcatc tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac
     5641 cgcattggtg tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta
     5701 atcccacagg ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg
     5761 catattcact tccaggaagt tatacgggac tttattgata aagcctggtt gttcaccctg
     5821 aatggtaccg aggatggtaa caaagtgatt acgatagttc actggatcaa ggaagccgct
     5881 ttgtctggcg agcagtcggc cctgatagtt ggcttcaata tcaggcttcg catagctatc
     5941 caacggtaat acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac
     6001 cttcccacca aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac
     6061 aaaacttttt tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca
     6121 tgcggcaagc aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact
     6181 atccttatca acaaatatta aaaccataat agatatttct ttatagtttt catctgattc
     6241 tgaggtgata tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttctgtttag
     6301 ttcaattccg ttattttggg gcgtggtatg agtatccatc gcccaataat ataacccttt
     6361 gttttaaaat tacataaaat ctatttttat tcaatgatta taaagtgcta ataatcaata
     6421 ttatggtatt atattctgat aatgatttga taagcgaatt cttttgccag atggtatttc
     6481 tatttccacc ccataattta tatctttttc attaggcaga tctctcttta aataaaaggg
     6541 gtatgaaatc ttttcgggtt gaagtgtctc gtaatgaaat tctatgaatt catttttaga
     6601 cattgttgcc catttttgaa ttttattgta tagagagggt gttgtaaact cttgaagttg
     6661 tttataacat atttttttga actcttgctt atattttaat atgtcatcaa atgaaagatt
     6721 cactaccttt gcctctaaga aatcaattat tctcgaagca atttccgctt tcgcgcgtaa
     6781 agagttgtca ttaatagtta acaatgtaga aaacaaacca ttgatttgtt tgtcaagatg
     6841 ttgttcatat ttatgtagtt tggggatggg attttccttg tgtgaaaaaa tagtgttaaa
     6901 taaatttgcg tcagtgaaat ttatattaag tagatcttca aaataaaatt ttgataaggt
     6961 taggactagc ttggacatat cttgccctgt catgttgaaa tcaataaagt cgcatccagc
     7021 aagggcttcc atcctatttt catttttact gtgctcaata tacagcttcg ccatttttaa
     7081 gtacaagctt tgaatgtctc ttatatcagt tggtaagtca gctcgttgcc cagcaaaatt
     7141 tttattatcg gaagaaacgg tttggacact gaatctttcg ggtattcctt cttctacgag
     7201 aattttagtt atgattggat tgttgggttc tggattaacg acaacaatga tttgacatac
     7261 agaatcctta tccttttcgt atctaatgtt atgaatctga ttaagtattg attgtgtctg
     7321 ttgcgtaagt ggtgtcgaac ttatcgtttc aatgttatta ataagactta caagttgaac
     7381 cacaactttt gagtagtctg catttgtata tgtggtgttc gtgtggcatg cattaatttg
     7441 ttggagttgt tgttgcgcta aatcaaattg ttcagggttg tatccttctc cagagaatgt
     7501 ggaattaatg ggttgttgtt cttcccaatt atcacaaggc cacttcggtg tgtctggcgt
     7561 tttaacatta tcggtatttg ttgtgcagtt ataaatatcg ccgtgatatg tatttattat
     7621 acctggggta aagtcaattg acataacatt ccttattcat catctgtttt atgctaatgt
     7681 ttttaaattg gataattaga acaggaatgg attttcttct acacacaata aatataatgc
     7741 gttatatctt cctgatataa ttacctttaa attccctttc atttccaccc ggtaatattt
     7801 gtgtataaaa aaagggcagt gatagtatcg taaaggaaaa cgttaacaac tatcaaaaaa
     7861 cgctcatttt aaaaagttat tatgttctcc gatagtgtgt gaagtaatat ttttgtgatg
     7921 ttttagatgt gtaataatga accaggttaa agtgtattta aaaatgtgtt gtattagcga
     7981 ctgaaatctg ccttttaaaa aggaggaggg tactgagaca aagagtcatt gcttctccga
     8041 taaaatgtca gagaagcatg attacgtggt tattgtctgt tgatgacttg ttgaccgagc
     8101 cgtcatcatt ctcaatgcat tagttaaatc atttgtgatt tttaaggatt atcctgtcta
     8161 tgattttcta cttagaagga tatggcacta actatgcatc ttatagttta ctgctggcat
     8221 ctatttaaaa tagcaattga gtcaaatagc ccatcaagat agccatacct aatataaccg
     8281 tcaggaatgc agctatcatc ggcattctga acattgattt aagtaatatc acttccgtca
     8341 ggcttgcgcc ggcactgccg atgattaatg ccattaatgc tcccagaccc attccttttg
     8401 tcatcagaac agatgccaga gggataaccg cctcagcccg gatatacagc ggaataccga
     8461 caacggcgct taatgggatg gcgaagggat tatctgctcc tgcatgagcg gcaatccacg
     8521 ctgagggaat aaagccataa ataaaagatc ctattaatac tcctaacaaa agatatggca
     8581 gtacatcttt aaactgttgc aacgcatctt tggtggctag ttttatggga ttaatactta
     8641 ctacagcttt ggtactgcaa caggtaacta cggggttctc aatggcttta gccgctggac
     8701 tacaacaact cacttttatg ggcgtatatg tcgttcccgg cgaagttttt gctggagcac
     8761 aacaatttgc tgatgagctt ttactggcaa tgatatgacg ttcaaacccc agagaatcca
     8821 ggataatact ggcaagaacg gagacgccgg ctgcgataat cgcgtacaac aaggtgactt
     8881 tccaaccaaa ggtcatccac attagtccga cgataatggg attaagtaat ggtgaaacaa
     8941 ataaaaaagt gagagtcgga ccaaacccgg ctttcgctga caacaatcca cgtaacatgg
     9001 gtattgtcga gcaactacag aacggggtaa cggctcccaa cagagcagcc aggagatagc
     9061 ctctcccttt tctggccccc atcatctgct ggattttatg gtctggcacc ttttgtctta
     9121 tcaggctgac accggcacta atcactataa acagcaacga aagctcgacg gcgagaaaca
     9181 caaacatctc t
//
LOCUS       DAJTSA010000142         9114 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000142 DAJTSA010000000
VERSION     DAJTSA010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.142"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(98..1474)
                     /gene="trkA"
                     /locus_tag="OO369_003690"
     CDS             complement(98..1474)
                     /gene="trkA"
                     /locus_tag="OO369_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR5983811.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1496..2785)
                     /gene="rsmB"
                     /locus_tag="OO369_003691"
     CDS             complement(1496..2785)
                     /gene="rsmB"
                     /locus_tag="OO369_003691"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR5983812.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2831..3778)
                     /gene="fmt"
                     /locus_tag="OO369_003692"
     CDS             complement(2831..3778)
                     /gene="fmt"
                     /locus_tag="OO369_003692"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR5983813.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3793..4302)
                     /gene="def"
                     /locus_tag="OO369_003693"
     CDS             complement(3793..4302)
                     /gene="def"
                     /locus_tag="OO369_003693"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR5983814.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4467..5591
                     /gene="dprA"
                     /locus_tag="OO369_003694"
     CDS             4467..5591
                     /gene="dprA"
                     /locus_tag="OO369_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR5983815.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5563..6036
                     /locus_tag="OO369_003695"
     CDS             5563..6036
                     /locus_tag="OO369_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR5983816.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6065..6607
                     /locus_tag="OO369_003696"
     CDS             6065..6607
                     /locus_tag="OO369_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCR5983817.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6612..7184
                     /gene="tsaC"
                     /locus_tag="OO369_003697"
     CDS             6612..7184
                     /gene="tsaC"
                     /locus_tag="OO369_003697"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR5983818.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7189..8007
                     /gene="aroE"
                     /locus_tag="OO369_003698"
     CDS             7189..8007
                     /gene="aroE"
                     /locus_tag="OO369_003698"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR5983819.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            8004..8261
                     /locus_tag="OO369_003699"
     CDS             8004..8261
                     /locus_tag="OO369_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR5983820.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8237..8791)
                     /locus_tag="OO369_003700"
     CDS             complement(8237..8791)
                     /locus_tag="OO369_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR5983821.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2286 a         2212 c         2274 g         2342 t
ORIGIN      
        1 tctaacaaat gttaagccat tttcctgcca ggaatgaatt taattaatcc ttgataatca
       61 aagggaaata ataaggcgtc attagacgcc ttattaatta caagaagaaa gggcttggct
      121 ggaagagtct ttcgacgtcg gtaataaact ttttatctgt gaggaacata attacgtgat
      181 cgccttgctc aatgcgcaga ttgtcattgg caatcatcac gtcgtttcca cgtaccaccg
      241 ctccaataat cgttcctggc ggtagcttga tttcgtcaat gactctgccg acaacgcgtg
      301 aggtgctttc atcaccgtga gcaacggctt caatagcttc tgctacgccg cggcgcaatg
      361 aggaaacacc aacaatatct gcttttcgca catggctaag caacgcagaa atagttgctt
      421 gttgtggtga aattgcaata tcgatcacgc tcccctgaac cagatccaca taagcgcgac
      481 gctggatcaa taccatcacc tttttcgcac ccatacgttt ggcaagcatg gcggacatga
      541 tattggcctc gtcatcgttg gtgacggcaa taaacagatc aacttgatcg atatgttctt
      601 cggccagcag ttcttgatcc gacgcatcac caaaaaagac gatcgtattc tgcaactttt
      661 ccgccagttc ggcagcgcgc tgctgattac gttcgatgag tttaacgctg taatcttttt
      721 ccagacgacg cgccagccct gcaccgatat taccgccacc aaccagcatg atccgtttat
      781 acggtttttc cagtcgctgt aattcactca tcaccgcgcg gatatgctgt gaagcggcaa
      841 taaagaacac ttcatcacca gcttcaacaa tggtcgaacc ttgtggacga atggggcgat
      901 cgtggcggaa aattgctgcc acacgagtat cgatatgtgg catatgttcg cgcatggtcg
      961 aaagtgcatt accaatcagc gggccgccat aataggcttt aactacagcg aggctgactt
     1021 taccctcagc gaagttcacc acctgcaatg cgcctggata ctcaatcagt cggtaaatat
     1081 tatcgataac caactgctct ggtgcgatca gatgatcaat cggtacagca tctgaatgaa
     1141 atagcttatc ggcatcgcgt acgtagtctg gtgagcggat gcgagcgatg cgattagggg
     1201 tgttgaaaag tgagtaggct acctggcagg caaccatatt ggtttcatct gaactggtta
     1261 cagcaaccag catatcggcg tcgtcggcac ctgcctcccg caatacgcgt ggatgagagc
     1321 catgcccctg cacgactcgc aggtcaaatt tatcctgcaa ggtccgcaga cgctcaccgt
     1381 tggtatcgac aacagtaata tcgttgttct cgccaaccag gttttccgcc agtgtgccgc
     1441 caacctggcc ggcacccaga atgataattt tcatcagtcg cgacccgtta tctcatcact
     1501 ttttgattag cttagcgtaa aagaagccgt cgccctcttc ggcaccaggc tgattttgtt
     1561 tacccggttg ctctggtgtt cctgtttcgc aaagttcggc atcagcggta cgttgcaaaa
     1621 aggctttaat ctgcaggcta ttctcttccg gtaacaccga acaggtggca tagaccaggg
     1681 ttccaccgga ttttaaatgc ggccaaatgg cgtcgagaat ttcagactgc aattgcgcga
     1741 gttccgggat atcgcgatcg cgacgtaacc atttaatatc tggatggcga cgaatcaccc
     1801 ccgttgctga acaaggcgca tctaataaaa tgcgatcaaa ctgttgctcg ccacaccatt
     1861 gggaagggta acggccatca ccttgtttca cggttgcctt cataccaagg cgttttaaat
     1921 tgtcgtaaat gcgagagagg cgctgttcgt caatatcaac cgcaacaacc tgcgcttctg
     1981 gtgccacctc aaggatatgc gttgttttac cgccgggggc ggcacaaaga tccaagatgt
     2041 gttcaccgtt ttgtggcgca agccaggtca tgcaaccttg tgctgatgca tcctgaacgg
     2101 taacccatcc gtcttcaaaa ccaggtagcg catgaacagg tgcaggtgtt tccagccgta
     2161 cagcatcagg gtaatccgca tgcgggaaac ctttcattcc tgcttcatcc agcaatgcaa
     2221 gccagctgtc gcgggaatga tgcgtacgat taatacgcag ccacattggc ggacgctggt
     2281 tattggcttc ggcgatggat tgccactgct ctggatacgc tttttgcaga cgcttcagca
     2341 accaggaagg atgcagataa cgtgcatcac gggtattaaa ctcggctaat aactcttctt
     2401 gccgacgctg gaactggcgt aatacaccgt taatcaaccc tttaagttgc ggacgcttaa
     2461 ttgcgatagc gccttcaacc gtttcagcca gcgcagcatg aggtggaatg cgggtataaa
     2521 gcagttgata caaaccaacc ataatcaggt aatgcacagt gcgctgtttg ccggtcatcg
     2581 gacgggccat taacttatta atcagccagt ctaactgcga aagcgtacgc agtacgccaa
     2641 agcacaactc ttgaagaagt gctttgtctt tatcggaaac tttttgctgg agcggtggca
     2701 gaatgttgct taatgattgc ccttgctcga cgacttgttc aacggcctgg gccgccatgc
     2761 tacgtaaatt acgttgtttt ttcataagta taaaaatgct cggcaagacc gggcttagaa
     2821 gagtggacta tcagaccaga cggttgcccg gaacaaacca ttcccgacga gagttcagga
     2881 ggtcttgcgc gctcatcgct ttcttacccg caggttgtaa cgagagcagg ttcaggatgc
     2941 catcaccagt cgcaacctga atgccttgtt tgttggcttc aaggatcgtt cctggtgcag
     3001 cgttggttgc cgtatcaatg accgacgctt tccagacttt aaccggctgt ccttcaattt
     3061 ccagccagct cattggccat gggttgaaag cgcgaatgca gcgttcaagc tgtgctgccg
     3121 aaagtgacca gtcaatacgc gcttcttctt tactcaactt ctcggcgtaa gtgacaagag
     3181 tttcgtcctg aacttctggt ttcgccgtgc cgtctgccaa ttgtttcaac gtggtgataa
     3241 gcccttgtgg gccaagctct gccagcttgt cgtacagcgt accactggta tcttctgcag
     3301 taatcgggca ggagagctta tagagcatgt caccggtgtc taaaccaaca tccatttgca
     3361 taatggttac accagtttct gcatcacccg cccatagtga gcgttggatt ggtgcagcac
     3421 cgcgccagcg tggcagcagt gaaccatgaa tgttgataca gccaagacgc ggcatctcca
     3481 gcacagcttt aggcagaatt aaaccatagg cgacgacgac cataacatca gcctgcaaat
     3541 cggcgaccag ttgctggttt tcttgtggac gcagggaaac aggttgaaaa acgggcagac
     3601 cttcttcctc agccagaact ttaatcgggc tgggcatcag ttttttaccg cgtcctgccg
     3661 gtcggtctgg ctgggtgaac acgccaacga cgttatgacc agaagacaac agcgcgtcga
     3721 gatgacgcgc tgcaaagtca ggtgtacccg caaaaataat acgtagtgat tctgacacgt
     3781 tagttcttat ccttaagccc gggctttcag acgatccagt ttttcaactt tctgacgaat
     3841 tcgttgttgt ttcagcggtg acagataatc cataaacagt ttgccaacca ggtgatccat
     3901 ctcatgctga atacagatag ctaacagacc ctctgcttcc agttcaaatg gtttaccgtc
     3961 gcggtcaagg gcgcgaattt taactttctc tgcgcgcggc actaaagcac gttgttcagg
     4021 gatcgacagg caaccttctt caatgcctgt ttcgccgctt ttttctaaaa gctccggatt
     4081 gattaacacc agccgttcgt cacggttttc cgaaacatca ataacaatga tacgttgatg
     4141 gatatcaacc tgggttgccg ccaggccaat accttcttct gcgtacatcg tctcgaacat
     4201 atcatcgacg atacgctgaa tttctgcatt cacttcttct accggtttag caactttgcg
     4261 aagccgctcg tccggaatat gtaacacttg caaaactgac ataaatctcc agagatgtgt
     4321 tcaggagtta gaaagattat ttcttctatt ctagacaaat ccccctctga ttgacagcat
     4381 cacttgattg acagcatcac ttgattgaca gcatcactga ccaatcgcaa agattgctaa
     4441 ggcttgctta tggcagggag ataaggatgg tcgatataga aatttggctg cgtttaatga
     4501 gcatcagcag cttgtacggc gatgatatgg tccgtatcgc tcactggctg gcaaaacagt
     4561 cgcatattga tgcggttgta ttgcagcaaa cagggcttac attgcggcag gcacaacgct
     4621 ttctttcatt tccacgaaag agtatcgaaa gctcactttg ttggttggag caacccaacc
     4681 atcatttaat ccctgcggac agcgaatttt atcctcctca acttcaggcg acgacagatt
     4741 accccggcgc actgtttgtt gaaggagaac tgcacgcgct gcattcattt cagcttgccg
     4801 tagtggggag tcgggcgcat tcatggtatg gcgagcgatg ggggcgatta ttttgcgaaa
     4861 ctctggcgac gtgtggagtg acaattacga gtggactggc gcgtggaatc gatggtgtgg
     4921 tgcataaagc ggccttacag gtaaatggcg tcagcattgc tgtattgggg aatggactta
     4981 ataccattca tccgcgtcgc catgcccgac tggctgccag tctgtttgaa cagggtggcg
     5041 ctctcgtctc ggaatttccc ctcgatgttc caccccttgc ttacaatttc ccacgaagaa
     5101 atcgcattat cagtggtcta agtaaaggtg tactggtggt ggaagcggct ttgcgcagtg
     5161 gttcgctggt gacagcacgt tgtgcgcttg agcaggggcg tgaagttttt gccttgccag
     5221 gtccaatagg gaatccggga agcgaagggc ctcactggtt aataaaacaa ggtgcgattc
     5281 ttgtgacgga accggaagaa attctggaaa acttgcaatt tggattgcac tggttgccag
     5341 acgcccctga aaattcattt tattcaccag atcagcaaga cgtggcattg ccatttcctg
     5401 agctcctggc taacgtagga gatgaggtaa cacctgttga cgtcgtcgct gaacgtgccg
     5461 gccaacctgt gccagaggta gttactcaac tactcgaact ggagttagca ggatggatcg
     5521 cagctgtacc cggcggctat gtccgattga ggagggcatg ccatgttcga cgtactaatg
     5581 tatttgtttg aaacctatat tcacactgaa gctgagttgc gtgtggatca agacaaactt
     5641 gaacaggatc ttaccgacgc aggttttgag cgagaagata tctacaatgc cctgctatgg
     5701 ctggaaaaac ttgctgatta tcaggaaggg ttggcagaac cgatgcaact ggcctcggat
     5761 cctctctcca tgcgtattta tacaccggaa gagtgtgaac gactggatgc cagctgccgt
     5821 gggtttctgc ttttccttga gcagattcag gtgctcaacc ttgaaactcg tgaaatggtg
     5881 atagagcgag tgctcgcgct ggataacgca gagttcgaac tggatgatct gaaatgggtg
     5941 atcctgatgg tgttgttcaa catcccgggc tgcgaaaatg cgtaccagca aatggaagaa
     6001 ttactctttg aagtgaatga aggtatgctg cattaatcat ttctttaatt cagcataagt
     6061 tgttatggca aaatcagcac tgttcacggt gcgtaataat gagtcctgcc caaagtgcgg
     6121 ggctgaactg gttattcgat ccgggaaaca cggtccgttt cttggatgct cacagtatcc
     6181 ggcgtgtgac tacgtccgtc ctctgaaatc ttcagcggat ggacatattg tcaaagttct
     6241 ggaggggcag gtttgccccg tatgtggcgc aaatctggta ttacgccagg gacgctttgg
     6301 tatgtttatt ggttgcagta actaccctga atgcgaacat accgaactta tcgataaacc
     6361 ggacgaaaca gcaattacat gcccccaatg tcggacgggc catctggtcc agcgccgctc
     6421 ccgttatggc aaaacatttc actcctgtga tcgctacccg gagtgtcaat ttgccattaa
     6481 cttcaaacca atagctggag aatgccctga gtgtcattat ccgctactca tcgaaaagaa
     6541 aaccgcgcag ggtgtaaaac acttttgtgc cagtaaacaa tgtggaaagc cggtttcggc
     6601 ggaataataa cgtgaataat aacctgcaag gagacgctat cgcagctgcg atagatgtcc
     6661 tcaatgaaaa acgtgtcatc gcctatccaa cggaagccgt tttcggtgtc gggtgcgatc
     6721 ctgatagcga aacagcagtg atgagactgt tggagttaaa acagcgtccg gttgataagg
     6781 ggctgatttt aatcgcggca aattacgagc agcttaaacc ctatattgat gacaccatgt
     6841 tgactgacgt gcagcgtgaa accatttttt cctgctggcc aggtcctgtc acctttgtct
     6901 ttcccgcgcc tgcgacaaca ccgcgctggt tgacgggccg ctttgattcg cttgctgtac
     6961 gagtcaccga ccatccgttg gtggttgctt tgtgccaggc ttatggtaaa ccgctggttt
     7021 ctaccagtgc caacttgagt ggattgccac cttgtcgaac agtagacgaa gttcgcgcac
     7081 aatttggcgc ggcgttcccg gttgtgcctg gtgaaacggg ggggcgttta aatccttcag
     7141 aaatccgcga tgccctgacg ggtgaactgt ttcgacaggg gtaacataat ggaaacctat
     7201 gctgtttttg gtaatccgat agcccacagc aaatcgccat tcattcatca gcaatttgct
     7261 cagcaactga atattgaaca tccctatggg cgcgtgttgg cacccatcaa tgatttcatc
     7321 aacacactga acgctttctt tcgtgctggt ggtaaaggtg cgaatgtgac ggtgcctttt
     7381 aaagaagagg cttttgccag agcggatgag cttactgaac gggcagcgtt ggctggtgca
     7441 gttaataccc tcaagcggtt agaagatgga cgcctgctgg gtgacaatac cgatggtgta
     7501 ggcttgttaa gcgatctgga acgtctgtct tttatccgcc ctggtttacg tattctgctt
     7561 atcggcgctg gtggagcatc tcgcggcgta ctactgccac ttctttccct ggactgtgcg
     7621 gtgacaatca ctaatcggac ggtatcccgc gcggaagagt tggctaaatt gtttgcgcac
     7681 actggcagta ttcaggcgtt gggtatggac gaactggaag gtcatgagtt tgatctcatt
     7741 attaatgcaa catccagtgg catcagtggt gatattccgg cgatcccgtc atcgctcatt
     7801 catccaggca tttattgcta tgacatgttc tatcagaaag gaaaaactcc ttttctggca
     7861 tggtgtgagc agcgaggctc aaagcgtaat gctgatggtt taggaatgct ggtggcacag
     7921 gcggctcatg cctttcttct ctggcacggt gttctgcctg acgtagaacc agttataaag
     7981 caattgcagg aggaattgtc cgcgtgaatc aggccatcca gtttccggac agggaagagt
     8041 gggacgagaa taaaaaatgt gtatgttttc ccgctctcgt gaatggtatg caactgacat
     8101 gcgcgatctc tggcgagagt ctggcgtatc gctttactgg agatacgcca gaacagtggt
     8161 tagcgagttt tcgtcagcat cgctgggacc tggaagaaga agcggaaaac ttaattcagg
     8221 aacaaagtga agatgatcaa ggctgggtct ggttaccctg atccagatat tcgtccttcc
     8281 atttcacgta attattcgcg gaatagcgta acccagcctt ctcttcatca cttaacgggc
     8341 ggatctgttt gacggggcta ccgagataca gatatccgct ctccagccgt ttattttgtg
     8401 ggaccagact acccgcacca atcatcacat catcttctac tattgcgcca tcaagtaaaa
     8461 ttgagcccat cccaaccaaa actcgattgc caatggtgca gccgtggagc atcaccttgt
     8521 gaccaacagt gacatcttcg ccaatggtta atgggttgcc atctgggttg tacgaggatt
     8581 tatgagtgac atgcaacata ctgccatcct ggatattggt gcgtgctccg atctgtacat
     8641 aatgtacatc tccacgaatc acaacgagcg gccagatccc cacatcatca gccagacgaa
     8701 cgtcaccaat cacgacactg ctatcgtcga tcattacgcg ctgaccgatt tgtggaaaaa
     8761 gatcgcggta tgggcgtaaa acatcagaca tacttacctc agcaataaat gatttactaa
     8821 tgactttggg ggcattattg gccttgtgca agtcttttag tatgcaaaaa agcaccgttt
     8881 tgtgtgcgat tgcagcaaaa agggtgaaaa aacaacaaac agaaaagaag atcaaaaaaa
     8941 tacttgtgca aaatattggg atccctataa tgcgcctccg ttgagacgac aacgtgaaac
     9001 acttcacagg atggtcggaa caacgaagag aaaaaatcct gaaattcagg gttgactctg
     9061 aaagaggaaa gcgtaatata cgccacctcg cgacagtgcg ctaaagcgcg tcgc
//
LOCUS       DAJTSA010000143         9060 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000143 DAJTSA010000000
VERSION     DAJTSA010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9060
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.143"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..923
                     /locus_tag="OO369_003701"
     CDS             <1..923
                     /locus_tag="OO369_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5983822.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="OO369_003702"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="OO369_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR5983823.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="OO369_003703"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="OO369_003703"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR5983824.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="OO369_003704"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="OO369_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR5983825.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="OO369_003705"
     CDS             4446..4805
                     /locus_tag="OO369_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR5983826.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="OO369_003706"
     CDS             complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="OO369_003706"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR5983827.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6059..6249
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6314..8158
                     /gene="btuB"
                     /locus_tag="OO369_003707"
     CDS             6314..8158
                     /gene="btuB"
                     /locus_tag="OO369_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR5983828.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8103..8960
                     /gene="murI"
                     /locus_tag="OO369_003708"
     CDS             8103..8960
                     /gene="murI"
                     /locus_tag="OO369_003708"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5983829.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2062 a         2191 c         2446 g         2361 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcgcc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg cgaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccatgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatctgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcatcgcgca gtgcttccgc ttcctgacgc cactcatcat ccagcttttt atggtacagc
     5581 agggaaacca ccgcctgatt actcagcgtg gtgaggtaat caatctggaa caacttgtgg
     5641 cgcagaacgg gattattacg cacacccgca atcatcgccg tcatcaactg gttgatgagt
     5701 tcactggcgg cggggaagct atccacgcgg atgcggcttt tggtttgttg atcgaaaatg
     5761 atgtgataca ggtcatcgcc atcgtgccag atgcggaact ccgcgcgcat ccggtaatga
     5821 ctgaccggcg agcgaaacac ttccggaacc aggtcagaaa acggtgccat catactttgc
     5881 aaacgtacca ctttttcggc taactgcgct tcatactgtt ctgttggaag gtgttcgggg
     5941 gtcatgatgt atcctgaaaa attaaagtac gcggcgattg tagggattgc tcatcagatg
     6001 tccagatctt gatgaattcc tatttgtgag ctacgtctgg acagtaactt gttacaacct
     6061 gtagcatcca cttgccggtc ctgtgagtta atagggaatc cagtgcgaat ctagagctga
     6121 cgcgcagcgg taaggaaagg tgcgatgatt gcgttatgcg gacactgcca tccggtggga
     6181 agtcatcatc tcttagtatc ttagataccc ctccaagccc gaagacctgc cggccaacgt
     6241 cgcatctggt tctcatcatc gcgtaatatt gatgaaacct gcggcatcct tcttctattg
     6301 tggatgcttt acaatgatta aaaaagcttc gctgctgacg gcgtgttccg tcacggcatt
     6361 ttccgcttgg gcacaggata ccagcccgga tactctcgtc gttactgcta tccgttttga
     6421 acagccgcgc agcactgtgc ttgcaccaac caccgttgtg acccgtcagg atatcgaccg
     6481 ctggcagtcg acctcggtca atgatgtgct gcgccgtctt ccgggcgtcg atatcaccca
     6541 aaacggcggt tcaggtcagc tctcatctat ttttattcgc ggtacaaatg ccagtcatgt
     6601 gctggtgtta attgatggcg tccgcctgaa tctggcgggg gtgagtggtt ctgccgacct
     6661 tagccagttc cctattgcgc ttgtccagcg tattgaatat atccgtggac cgcgctccgc
     6721 tgtttatggt tccgatgcaa taggcggggt ggtgaatatc atcacgacgc gcgatgaacc
     6781 cggaacggaa atttcagcag ggtggggaag caatagttat cagaactatg atgtctctac
     6841 gcagcaacaa ctgggggata aaacacgggt aacgctgttg ggcgattatg cccatactca
     6901 tggttatgat gttgttgcct atggtaatac cggaacgcaa gcgcagacag ataacgatgg
     6961 ttttttaagt aaaacgcttt atggggcgct ggagcataac tttactgatg cctggagcgg
     7021 ctttgttcgc ggctatggct atgataaccg taccaattat gacgcgtatt attctcccgg
     7081 ttcaccgttg ctcgataccc gtaaactcta tagccaaagt tgggacgccg ggctgcgcta
     7141 taacggcgaa ctgattaaat cacaactcat taccagctat agccatagca aagattacaa
     7201 ctacgatccc cattatggtc gttatgattc gtcggcgacg ctcgatgaga tgaagcaata
     7261 caccgtccag tgggcaaaca atgtcatcgt tggtcacggt agtattggtg cgggtgtcga
     7321 ctggcagaaa cagactacga cgccgggtac aggttatgtt gaggatggat atgatcaacg
     7381 taataccggc atctatctga ccgggctgca acaagtcggc gattttacct ttgaaggcgc
     7441 agcacgcagc gacgataact cacagtttgg tcgtcatgga acctggcaaa ccagcgccgg
     7501 ttgggaattc atcgaaggtt atcgcttcat tgcttcctac gggacatctt ataaggcact
     7561 aaatctgggg caactgtatg gcttctacgg aaatccgaat ctggacccgg agaaaagcaa
     7621 acagtgggaa ggcgcgtttg aaggcttaac cgctggggtg aactggcgta tttccggata
     7681 tcgtaacgat gtcagtgact tgatcgatta tgatgatcac accctgaaat attacaacga
     7741 agggaaagcg cggattaagg gtgtcgaggc gacagccaat tttgataccg gaccactgac
     7801 gcatactgtg agttatgatt atgtcgatgc gcgcaatgcg attaccgaca cgccgttgtt
     7861 acgccgtgct aaacagcagg tgaaatacca gctcgactgg cagttgtatg acttcgactg
     7921 gggtattact tatcagtatt taggcactcg ctatgataag gattactcat cttatcctta
     7981 tcaaaccgtt aaaatgggcg gtgtgagctt gtgggatctt gcggttgcgt atccggtcac
     8041 ctctcacctg acagttcgtg gtaaaatagc caacctgttc gacaaagatt atgagacagt
     8101 ctatggctac caaactgcag gacgggaata caccttgtct ggcagctaca ccttctgaac
     8161 cacgtcccac cgtgctggtg tttgactccg gcgtcggtgg gttgtcggtc tatgacgaga
     8221 tccggcatct cttaccggat ctccattaca tttatgcttt cgataacgtc gctttcccgt
     8281 atggcgaaaa aagcgaagcg tttattgttg agcgagtggt ggcaattgtc accgcggtgc
     8341 aagaacgtta tccccttgcg ctggctgtgg tcgcttgcaa caccgccagt accgtttcac
     8401 ttcctgcatt acgcgaaaag ttcgacttcc cggttgttgg tgtcgtgccg gcgattaaac
     8461 ctgctgcacg tctgacggca aatggcattg tcggattact ggcaacccgc ggaacagtta
     8521 aacgttctta tactcatgag ctgatcgcgc gtttcgctaa tgaatgccag atagaaatgc
     8581 tgggctcggc agagatggtt gagttggctg aagcgaagct gcatggcgaa gatgtttctc
     8641 tggatgcact aaaacgtatc ctacgcccgt ggttaagaat gaaagagccg ccagataccg
     8701 ttgtattggg ttgcacccat ttccctctac tacaagaaga actgttacaa gtgctgccag
     8761 agggaacccg gctggtggat tctggtgcag cgattgctcg ccgaacggcc tggttgttag
     8821 aacatgaagc cccggatgca aaatctgccg atgcgaatat tgccttttgt atggcaatga
     8881 cgccagaagc tgaacaatta ttgcccgttt tacagcgtta cggcttcgaa acgctcgaaa
     8941 aactggcagt tttaggctga tttggttgaa tgttgcgcgg tcggaaaatt attttaaatt
     9001 tcctcttgtc aggccggaat aactccctat aatgcgccac cactgacacg gaacaacggc
//
LOCUS       DAJTSA010000144         8992 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000144 DAJTSA010000000
VERSION     DAJTSA010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8992
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.144"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(141..2684)
                     /gene="mdoH"
                     /locus_tag="OO369_003709"
     CDS             complement(141..2684)
                     /gene="mdoH"
                     /locus_tag="OO369_003709"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR5983830.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2677..4212)
                     /gene="mdoG"
                     /locus_tag="OO369_003710"
     CDS             complement(2677..4212)
                     /gene="mdoG"
                     /locus_tag="OO369_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR5983831.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGCDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4606..5763
                     /gene="mdoC"
                     /locus_tag="OO369_003711"
     CDS             4606..5763
                     /gene="mdoC"
                     /locus_tag="OO369_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR5983832.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5771..7252)
                     /gene="clsC"
                     /locus_tag="OO369_003712"
     CDS             complement(5771..7252)
                     /gene="clsC"
                     /locus_tag="OO369_003712"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR5983833.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7194..7727)
                     /gene="ymdB"
                     /locus_tag="OO369_003713"
     CDS             complement(7194..7727)
                     /gene="ymdB"
                     /locus_tag="OO369_003713"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR5983834.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7822..8133)
                     /locus_tag="OO369_003714"
     CDS             complement(7822..8133)
                     /locus_tag="OO369_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5983835.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8254..8586)
                     /gene="csgC"
                     /locus_tag="OO369_003715"
     CDS             complement(8254..8586)
                     /gene="csgC"
                     /locus_tag="OO369_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR5983836.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8645..>8992)
                     /gene="csgA"
                     /locus_tag="OO369_003716"
     CDS             complement(8645..>8992)
                     /gene="csgA"
                     /locus_tag="OO369_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCR5983837.1"
                     /translation="NNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHGGGNGAD
                     VGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVT
                     QVGFGNNATAHQY"
BASE COUNT         2250 a         2328 c         2132 g         2282 t
ORIGIN      
        1 aacgccggta tttttattgg ctcgttgttt ttgccggatg tggcgtgaac gccttatccg
       61 acctacaaaa tcttgcaaaa tcaataaatt gcaggaacga tgtaggcctg ataagcgtag
      121 cgcatcaggc aactacgttt ttattgcgaa gccgcatccg gtttacgcaa tgccagtgga
      181 ttgagcttta tcccttcgta ataactcacc catgaagaat atctttcagg ggaattccag
      241 acgcggaaat gcagacgggc catcgtcacc ggatcgctta gcagcaccag gcgacgatcg
      301 cgattcagct tctctggcgt ctcgttaagc gcctgttcaa cgtggcggtc acgggcgatt
      361 tccagcacct tactggcgcg gtgacgcgct gtcgccattg cggttgccag agcgttaaat
      421 gacgggttaa acactgcgtg cataaagcca tcgtcaaggg agcgttggcg attcatctca
      481 aggaaccgat cggtatcaac cagcacctgc ggcggtgaat actcttccgg gatcaggaac
      541 agtttccagc gtttggtgcg cagaccaacg gtggcacggc tggaaatcac cgaaacaaac
      601 ggtgacagga tcaacgagaa gacaatcggt gccagccaga acaggaaacg cagatccagc
      661 cacgccatcc caacagccca cactaacccc aacagcagct gtgagccgtg gcgtttgaac
      721 gcttcgcccc atgaagtgga gtcatcatca cgctgcggtg aattccacac cacttcccag
      781 ccaaggaacg cgctgacaac gaagaccgta tggaacagca tgcgtaccgg agccagcagc
      841 acggaaaaca gcacttccag cagcaacgat aatgtaacgc gccagaagcc gccgtattct
      901 ttcgttcctt tgcaccagat aagcaaaatg ctcaataact tcggcaggaa caacagcacc
      961 atggtcgaag caaaaagtgc aatcgccagc tcaggacgcc actgcggcca caccgggaac
     1021 aactgccgtg gttgcaggaa gtattgcggt tcggtcaacg catgcactac ctgcaatgca
     1081 gtagagagcg cgaggaacat aaaccacagc ggagcggaga gataagacat cacgcccgtc
     1141 aggaacaccg cacggtgaac cgggtgcata cccttaacca ggaacagacg gaagttcatc
     1201 aggttaccgt ggcaccagcg gcggtcacgt tttagctcat caagcaagtt aggcggcaat
     1261 tcttcataag aacccgggag atcgtaagca atccagaccc cccaacctgc acggcgcatc
     1321 aacgccgctt ctacgaagtc atgtgacagg attgaaccgg caaaggaacc ttcgcccggc
     1381 agcggagcca gtgcgcagtg ctcgataaat ggtttcacgc ggataatcgc gttatgtccc
     1441 cagtagtgcg actcgccaag ttgccagaag tgcaaaccgg ctgtaaacag tggcccatac
     1501 acgcgggtcg cgaactgctg acagcgcgca tacagcgtat ccataccgga cgctttcggc
     1561 gacgactgaa tgatcccggc gttcgggttg gcttccatca agcgcaccag gccgcataaa
     1621 caatcaccgg tcattaccga gtcagcatcc agcaccacca tgtagctgta ctggctgccc
     1681 cagcgacggc agaagtcatc gatattaccg cttttacgct tcacgcggcg acggcggcgg
     1741 cgatagaaaa tctgaccttc gccaccgact tcagcgataa gctccatcca ggctttttgc
     1801 tctgcgacgc agatatccgg gttataactg tcactcagaa tgtagacatc aaagtgtttg
     1861 gcattcccgg tggcttttac tgattcccac gttgcacgca agccagcaaa aacacggttc
     1921 acgtcttcgt tacagatagg catgatcaac gccgtgcgat gctccgggtt taatggttca
     1981 tcgccaactg ttgacgcaga tatactgtat ttatcgcgac caataagcag ttgcaggaag
     2041 cccatcaacg ccgtccagaa tccggcggac acccaacaga acagtaccgc aaaaaggatc
     2101 aggataccgg tttgcagcat ataaggcaga agctgcataa aggaaaccca caaatcctga
     2161 ccaaccatat ccataggatt aatcagcgcc caaccctgat aaggaagaat ggtcttcata
     2221 taccaggtcg cgacaacagt ttgcgcgagc gtcaggatca acagaatgta acggcggatg
     2281 gtaccaacgg tacgccactt ttgctcgctc tcctgctctt ctttggtcaa acgagccaga
     2341 tagcgcggcg tgacatcgcg tccacgcagg cgatcccaga aacggcctac cgggttggta
     2401 cgccacgggt cgggaaacat cgaggagcgt tttgcttctg gcatcgcctt aagctgatcg
     2461 cgcccttcgt cgtctttaat taactgtcca tcagcaagtg aatctggcca ggcttgttcc
     2521 agacgcgcct ttaccgagcc ttgcggggaa tcatcctccc gcgcccaggt gcggtgttcg
     2581 gcatccagcg cctgatgaac ggcgcggata tcagtcttcg gcaatgctgc tttctcgctt
     2641 gcggcgatgg gcattgcgtc aatgtactca gttgtcttat tcattggcag gtaactggta
     2701 gctccaggtt tcactcaacg tctgatcggc attcaccagc gcagcacgca tttcagtgat
     2761 tttcttggca tctttcactt tcacacgcat caccagacgc cagcctttgg taaccgggtt
     2821 atagcgcacc gtgctttcaa ctatctcacc attatcacca atgctggttt gcgctgtgac
     2881 cggggtatcc tctggcagtt ttttcatctc tgcgccggta aaatcgacca caaaggcgat
     2941 agtaccgtca ggctggcgaa tcaggttcga ctgcttcaca tcccccgttg aacgacgcgt
     3001 ttgttgcacc catgcgttat ctggcgcatg cagtttgtct tcatcacggc tgaaggtgat
     3061 ggtgtattta aagttcatct ctttacccgg ctccggcagc tgatccggcg tccagtaagc
     3121 gacgatgtta tcgttggttt catcgttggt tggaatttcc accagctcaa cgctgccttt
     3181 gccccactcc cctttcggag tcacccatgc gcttggacgg agatcgtaac gatcatcgag
     3241 atcttcaaag cgggagaaat cacaacctcg ttgcaacaga ccaaagcctc gcgggttttc
     3301 catggagaag ctgctgaccg ctaaatgttt cgggttattc aacggacgcc agatccattc
     3361 gccattacca gcatggatag acagaccgtt agagtcgtgc aactccggac gatagttatt
     3421 tgcaggcgac ggttggttcg gcccaaacag gaacatactg gttaacggtg caacccccag
     3481 tttgccgact ttatcgcgca gatagatttt cgactgcaca tccacaaccg tgtcacgccc
     3541 tggcataacc acgaatttgt aagcacctgt cgcacgcggc gagtcaagca atgcataaat
     3601 ggttaaacgt ttatcagtcg gttttggacg ctcgatccag aactctttga agcgtggaaa
     3661 ttcttcaccc gatggcaaag cggtatcaat tgctaggccg cgggcagaaa ggccataaac
     3721 ctgacctgca ccaatcacgc ggaaatagct ggccccgagc atgctgacga tttcatcgtt
     3781 tttatctttg ctgttgatcg ggtaaagcac tttaaaaccg gcaaaaccaa ggtcttttac
     3841 cgtgtctttg tcatgctgaa catcgccgaa agtgaaataa tccgggctgt atttgattcg
     3901 tttgactgcg gtggcagtca cttcatttat tttgaccggg gtatcgaagt acataccctg
     3961 atggtagaac tcgagtttga atggggtctt cagattgttc cagtacgctt tgtcatgatt
     4021 aaactggatc tgctgatagt ccgcgtattt catgtcgcgg aaaacggagg gcaagttgct
     4081 tttaggcgcc tcatagcctt tcccggctaa ggattgagct tgctttgcga catcatcaat
     4141 actgaaagcc cagcttgaag atgtatacag ggttaacatt actgcagcac tcaaccaacg
     4201 cattttcatc atttgtagtt tatgtttcat aattagtaag cacttccccc tttgtgtgct
     4261 tatatcgatc cgatctattt taatggataa ccagccattc cgacaactga atcccggtat
     4321 cgttcagcac gctggctcat ggattaagca cctgattgca accgttttca ctttccgtgc
     4381 ttctcctaaa gacagaagca tcgacttcgt gtagggtttg ccagcaaatt tatttatcaa
     4441 ccgtcttaca gacgcttcca ttctggttag tggtagcgat gatgctatgc ataaaaacct
     4501 tgaagcctgc gcctggcgcg gtcaggtcgg tgtcgctatg gcaacaagcc taaaggcata
     4561 acccgacagg gataagacga aaaatccgag attagttaac catatatgaa tccagtaccc
     4621 gcgcaacgtg aatatttcct cgactccatc cgcgcctggc tgatgttgtt agggatccct
     4681 tttcatattt ctttaatcta ttcgagccat acatggcatg tgaatagcgc cgaaccgtca
     4741 ttgtggctaa ccctttttaa tgacttcatc cactcgttcc gcatgcaggt atttttcgtt
     4801 atatccggat acttttccta catgcttttt ttacgctatc ccttgaaaaa atggtggaaa
     4861 gtacgtgtcg aacgtgtagg tatcccgatg ttaacagcca tccccctact gacattaccg
     4921 caatttatta tgctgcaata tgtcaaaggg aaagcggaaa gttggcctgg gctatcattg
     4981 tatgacaaat ataatacgtt ggcctgggaa ttaatatcac acctgtggtt tttactggtg
     5041 ttagtagtca tgacgacgct gtgtgtatgg atatttaagc gcatcagaaa taatttagaa
     5101 aattctgata aaacgaataa aaaattctcg atggtaaaac tatcggtgat ttttttatgc
     5161 ctcggcatcg gctatgcggt aataagaaga acgattttta ttgtgtatcc acccattctg
     5221 agtaatggca tgttcaattt tattgtcatg caaacgctat tttatttacc gttctttatc
     5281 ctcggcgcac tggctttcat tttccctcat cttaaagcct tgtttaccac gccgtctcgt
     5341 ggctgtaccc tcgctgcagc attggcgttt gtcgcttatt tactcaacca gcgctatggc
     5401 agtggcgatg cctggatgta cgaaaccgag tcggtgatca ccatggtcct cggcctgtgg
     5461 atggtgaatg tggtcttctc ctttggccac cgtttgctta acttccagtc agcgcgggtg
     5521 acttattttg ttaacgcatc gctgtttatc tatctggttc accacccgtt aacgctgttt
     5581 ttcggcgcat acattacacc gcacatcacc tccaactggc ttggttttct ctgtggcctg
     5641 atattcgtag tagggattgc gataattctg tatgaaattc atttacgcat cccgttactg
     5701 aagtttttgt tctctggtaa accggttgtt aagcgtgaga acgataaagc accagcccgt
     5761 taagccacat ttacaataac cattccacgg gcaatatcga cgccagtctg accataaccc
     5821 gcttccagaa actggtggcg ggttcttttt tgagaataac ctctttcttt gcatgacgat
     5881 caacccagtt gatccgtccc cacctgtcca gacggagctg ccaggccgca tcgtactggc
     5941 tctgaataaa gcgtttatca attaactgtg ccagcgtttc gctctctatc acgaagccca
     6001 tttcagtatt gagcaatgtt gaacgcggat cgaaattgaa agagccgata aacaccgttt
     6061 taccatcgat gctaaaagtt ttagcatgca ggctggcacc ggaattaccg gttatgccgc
     6121 gatcgtgtaa cgtactgctt tgttcacgcg tcggcttgag ttcatataat tccacgccat
     6181 agcggagcaa ttttttgcgc cagcgcgcgt atccggcatg gacgacagca acatcgttag
     6241 cggcaagaga attggttagg atcgcaatct ttaccccttt tctcaccatc cgtaagagtt
     6301 gcgccacacc tgcgcgtgtc ggtacaaaat aggaagagat aatatcgatg cgttcactgg
     6361 gtgagcccat gatatcgaac aggcgctgcg gtaacagtga atgccgtttt gccttgccct
     6421 cccctttcgc cggatcatca cttaataaac gtgtcttcgc ccagataagc ggcaacgttc
     6481 catcaaccag atgatttata aatggactgg attccatttt gcgtaaataa cgatgcgtca
     6541 tggcatcgtt atgccaggag gcgggtaact cgatgcgatc cgccatttca ccttccggga
     6601 catccagcac ctgctgtaag ggtgaaaccg atttgcaata ccagtagcgg gcgaaatcat
     6661 cggcaacatc ctctaccacg gggcctattg ccatgacatc taaatccgaa aaaagtggct
     6721 cttcccctgc accaaaatag gcatcaccaa tatttcgtcc tcccactagg gtaaccacgc
     6781 catcgacagt gaaactttta ttgtgcatcc ggcgattgag acgggaaaag tcggtgatat
     6841 atccaagcgg acgtagcaag cgaaacgaga aaggattaaa aagccggact tcaatgcgtg
     6901 gatgactgtc aagcaagcgc aaaatgtcgt caagtccggg cgtattgttg tcgtccagca
     6961 acaaacggac gcgaacgcca cgctttgctg cggctaacag ggcggaaaac agtaaccgcc
     7021 ccgacatatc gtcctgccag atgtaatact gaacatccag cgtatgctct gccatttcgg
     7081 ccagacgata ccgggcggca aacgcatcca gacttttctc cagagggaaa agaccacact
     7141 gaccgggatg ttgcgaacac agtggcagca ccgcgctcgc cagccggggc aaatcattca
     7201 tctccttgtt gggtaaggag tctttcgtag aggtgggcgt tttcttcatc ataacagaca
     7261 aagtatacct gttcgggtaa agcgtgacgg gtaataaatt ctgaaacggt tttaactgcg
     7321 atttcagccg ctgccgcacg agggtaacta taaaccccag tactgattgc aggaaaagcc
     7381 actgacgtat agctgtttgc cgccaccagt cgtaggctat tcagataggc atcctgcaaa
     7441 agctggtctt cgttttgttc cccaccacgc cagactggcc cgacggtgtg tactacggct
     7501 ttagcgggaa gatcgcctgc aagcgtaata acggcatgcc ccgtagggca atcgccctgc
     7561 tgttgcctga cttttaaaca ggcatccagc agggccggac ccgctgcgcg atgaatggcc
     7621 ccatcgacgc cgccgcctcc cattaatgac ggattagccg cattcacaat cacatcaacg
     7681 gccagtttgg taatatcacc ctgcacaaca tgaatacgcg ttttcataac gcctccttac
     7741 accttcttgt ttcttaagtg tacagcaggc tttttggtta aatcgcattt ttctgctcag
     7801 caacgtgagt tgttcagaaa cttagttata ttcgatgttc attacagcca gctttttctg
     7861 tttatcaaga tactgcactt ttactgaggc tatttgcgcg tttttgacat tgccgctcat
     7921 taacgctttg ctggagtagg ttttgcccgg aatagaacca ttctgtgggc agttcatttc
     7981 tacgggtgat gactgggtgc tgacgtcaca tgccggttcc acaatttggc cataaaaatg
     8041 gatcacgccc ccttgcgcgg tgcttcctgc aatggcaata aaaggaaaaa acaaactgcc
     8101 gagcataaga gagtctaaaa aaggtctgaa catttattct atttcctcaa tgattagtca
     8161 tccttgaagg ttgtgttatc catactttca ggttggctga ttcgcagcag accattctct
     8221 ccagattcat cttatgctcg atatttcaac aaattaagac ttttctgaag agggcgtcca
     8281 ttgttgtgat aaatgaagtg cctgtccatc agaaacagta acaactattt tcacccgatc
     8341 ttccggggaa atatttaaac tcaacttcgt caaagcaatg ggttgattag caggcaatga
     8401 gagggtcttt tcttgcttcg tctgactttg ccctgaactg ccttcgcgca gggacaatat
     8461 ttgtactctg cacagacaag attgagtaag agtaacttca ggaataatgg tgtacatatc
     8521 cccttgctgg gtcgtattaa aggttatctg gctggaaagt gccgcaagga gtaataacgc
     8581 attcatattc ttctcccgaa aaaaaacagg gcttgcgccc tgtttctgta atacaaatga
     8641 tgtattaata ctgatgagcg gtcgcgttgt taccaaagcc aacctgagtc acgttgacgg
     8701 aggagttcga tgcagtctgg tcaactgcag caccgttgcc accaccgaac tgtttaacag
     8761 tcatttcaga atttttgccg ttccactgat caagagtggc gctgttaccg aagccacgtt
     8821 gggtcagatc gattgagctg tcatctgagc cctgaccaac atctgcacca ttaccaccgc
     8881 catgctgggt aatagtcaag tcagagttac gggcatcagt ttgcagagca agtgcagagt
     8941 taccgccacc gtactggtaa atgttcagct ctgaatttgg gccgctatta tt
//
LOCUS       DAJTSA010000145         8757 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000145 DAJTSA010000000
VERSION     DAJTSA010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8757
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.145"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            5..436
                     /locus_tag="OO369_003717"
     CDS             5..436
                     /locus_tag="OO369_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR5983838.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="OO369_003718"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="OO369_003718"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR5983839.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="OO369_003719"
     CDS             complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="OO369_003719"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR5983840.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="OO369_003720"
     CDS             complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="OO369_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR5983841.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     VPKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4438..5145)
                     /locus_tag="OO369_003721"
     CDS             complement(4438..5145)
                     /locus_tag="OO369_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR5983842.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="OO369_003722"
     CDS             complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="OO369_003722"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR5983843.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6003..6206)
                     /locus_tag="OO369_003723"
     CDS             complement(6003..6206)
                     /locus_tag="OO369_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5983844.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6395..7873
                     /locus_tag="OO369_003724"
                     /pseudo
     CDS             6395..7873
                     /locus_tag="OO369_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7870..>8757
                     /locus_tag="OO369_003725"
     CDS             7870..>8757
                     /locus_tag="OO369_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5983845.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEP"
BASE COUNT         2032 a         2387 c         2249 g         2089 t
ORIGIN      
        1 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt
       61 gttttatgtc gcgggagata aagtcggcat cgttcgtctg gatgctttta aagccagtaa
      121 agatattcag tttgcggtgc agtcaggacc aatgttgatg gaaaacggtg taattaatcc
      181 gcgtattcat cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca
      241 tgggaatgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
      301 tgccaaagcg aaactgaacg ttgagcagct gctttatctt ggtggcacga tttcacatat
      361 gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
      421 cgagcggaaa ggttagaatg atagctttgt gtcagatact aaatatgctg ctgatgccgg
      481 atgcggcgta aacgccttat ccggcctaca tgacgttgca atttattgaa tctgaatgat
      541 tttttaggcc aaataaggtg cgcagcaccg catccggcaa cgttacaccg actccagccg
      601 ggcgtaagct gccaccagcc atttgattcc ctggccctgg aatgccacct gcaaacggct
      661 atgttcaccg ctgccttcca tattgacgat ggtgccttca ccaaacttag cgtggcgtac
      721 acgctggccg agcttgtagc cgctgtcgtt ctcgaccatc ggcgtaccca tccgctgatg
      781 gctgaccggg cggcttaccg tggcgcgcag gcgcacctct tccacacact cttccggcaa
      841 ctcgccaata aagcgcgacg ggcgatggta aacctctttg ccatacagac ggcgagtttc
      901 cgcgtaggtc agcgtcagtt tctgcatcgc gcgggttacg ccaacgtagg ccagacggcg
      961 ttcttcttcc agacgcccgc cttcatccag cgacatctgg cttgggaaca tgccctcttc
     1021 cataccgacg ataaacacct gcgggaactc cagacctttc gccgagtgca tggtcatcag
     1081 ttgtaccgca tcctgccagg tatccgcctg cccttcgcct gcttccagcg ccgcatggga
     1141 taggaacgcc tgcagcggca ttaaatcttc gtcttcttcg ttgtagctga actggcgcgt
     1201 tgccgtcacc agttcctcta agttttcgat acgcgtctga cctttttcgc ccttctcctg
     1261 ctcgtacatg gtacgcaggc cggagtcttt aattacccgg tcagtctgta catgcagcgg
     1321 catatcggca gtttcctgcg ctaaggcgtc gatcagttcc ataaaccgct gtaaggcgct
     1381 ggcagcacgt ccggcgaggg ctttttcctg caacagttca cgacatgcct gccagagtgt
     1441 taactggcga tcgcgcgatg tctgacgtac cacgtccagc gtccggtcac caataccccg
     1501 cgttggcgta ttcaccacac gctcaaaggc cgcgtcgtcg ttacggttgg caatcaggcg
     1561 cagatacgag agcgcatctt tgatttcctg gcgttcgaag aagcgcatcc cgccgtaaat
     1621 acggtacggc atactcgcct gcaacagtgc ttcttccagc acacgcgact gggcgttact
     1681 gcggtagaga atggcgcact cggcaagcgc cccgccgttg tcctgccagg ttttgatgcg
     1741 gttaaccaca aaacgcgctt catcgagttc gttaaaagcg caatagaggg aaataggctc
     1801 accgtccgcg ccatcggtcc acagtttttt acccagacgc ccgttattgt tttcaatcag
     1861 ggcgttagcg gcgctcagaa tattgctggt agagcggtag ttttgctcca gacgaataat
     1921 ttcggcaccg gggaaatcat taaggaaacg ctgaatattc tccacctgcg ccccgcgcca
     1981 gccgtagatt gactggtcgt catcaccgac gatcatcact ttgccggtgt cgcccgccag
     2041 caggcggatc cacgcgtact gaatgttgtt ggtatcctgg aattcgtcca ccaggatatt
     2101 ggtaaaacgt tcgcggtagt gttgtaggat atgcggcttg ttaagccaca gttcgtgagc
     2161 gcgcagcagc agctcggcga agtccaccag gcccgcgcga tcgcacgctt cctgatacgc
     2221 ctgatacacc ttctgccagg tctgctccac cggattaccg tagctttgaa tatgatgcgg
     2281 acgcaggcct tcatctttct ggctgttgat gtaccacatt gcctggcgcg gcggccactg
     2341 cttctcgtcg aggttcatgg ctttgatcag acgcttaagc aggcgtagct ggtcttcgct
     2401 atcgaggatc tggaaatcct gcggcagatt ggcgtccata tggtgcgcac gcagcaggcg
     2461 gtgcgccagc ccgtggaagg tgcctaccca catgccgccc tggctggtgc ccatcagttg
     2521 tccgatacga tgacgcatct ccgccgccgc tttgttggta aacgtcacag ccataatcga
     2581 gtatggcgag cagttttcca cgctcatcag ccaggcgata cgatgcacca gtacgcgcgt
     2641 cttaccactg cccgcgcccg ccagcaccag aaggttgctg cgtggcgcgg ccaccgcttc
     2701 gcgttgtttg tcattaaggc tgtcgagcag gtaagaaacg tccattggca ccgccgcgta
     2761 aaaataggtt gggcggagaa gcgcaacgcc ctccgccaaa aagctgggta tatatacaga
     2821 tttgctgatt atatcagcga ggtcagagat gccaaccggg aaatttccag atgtggcagt
     2881 aaacggctgt cccaggtttg catcagatcg ccattttccg gtctgatcca acaagcctgc
     2941 attccgctgc gaattgcccc acccacgtca gtggtgagat cgtccccaac atgtaagatc
     3001 tcgccgatcg gcacgttgag tttttccgca gccaaaaagt acatatcgct gaacggtttt
     3061 gagcgcccgt gcgggccagc gcgcagcaca aactcaaaat aatcccccaa accaaacagt
     3121 tccggctggg cgttaccgtt ggtgatcgcc accagcggcc atttcttcgc cagctgtttt
     3181 aaggtgtcgt gagtttgctg cgggacgtcg attcggctgc gccatttggc aaagttaatc
     3241 attgctgcgt gtgcgcctgc actggcttct tcggcactca gcccggcgtc gagcatcgct
     3301 tgttcaatcg aacgaaaacg ccaacgtgtc acgtcgtgat aaatctcggg ttccgcttcc
     3361 cgtaccgcct ggcgcaggcg ttgcagatct tcattctgga agctgcgcag cgccggatga
     3421 taattttgca caaaggtaag cgcctctcgc tcggtgcgca aaatcaccgg acggttatcg
     3481 taaagggtat catccaggtc aaaggtgagc gccgagatgc gccccaaagg ccggtaaaaa
     3541 cgcattattt cccccgtttg gcgcgtggat gcgccgcatc gtacaccgag gcaaggtgtt
     3601 gaaaatcaag atgagtatag atttgcgtgg tggagaggtt ggcatgaccc agtagctcct
     3661 gcacaccacg aagatcgccg ctcgactcca gcatatgcgt agcgaacgag tgacgtaatt
     3721 tatgtgggtg aacgtgatta ttcagccctt gttttatgcc ccattcggca aagcgtttct
     3781 gcacattacg cgcggagata cgcttgccca gtttcgacag aaaaagcgcg tcgtcttcgc
     3841 taccaaacag gtcgcgcaaa tcaagccagt gctcaatcca cgccacagcg ttgcgaccaa
     3901 tcggcaggcg gcgctctttg ctgcctttcc ccatcaccca cacttcgccg gactccaggt
     3961 cgaggtgttt gatatccagc cccaccagct cggaaagacg cagacccgcg ccgtacatca
     4021 cttccagcat tgcacggtcg cgtacagcga ggggatcgtt gatatcaata tccagcagcc
     4081 gattcatatc gtcgacgtcg atatttttcg gcagatgacg cggcgctttc ggtaccgaaa
     4141 ctcctttcgc cgggttagct ttgagttcgt tctggctgac cagccagtca aaaaagctac
     4201 gtagcgcaga aagccgtaac gccagacttg ctgctcccag ccctttacgg cgactgcgta
     4261 cagcaaaatt gcgtaccatc gctgcatcgc attgttgcca gctttgcagg ccgttttcgc
     4321 tggcaaaatt gatgatcgcc tcaagctgac gctggtagtt aagtagggtt atcgggctaa
     4381 gctgacgctc cacgctcaga taacgtaggt agcgttctac atcggtgtgt aaatcggtca
     4441 tacgcgttca atccaacgct ccagaagctc cggcaacatc agcgcaattt catgaagtaa
     4501 ctgcgttccc tgcccttgtt gatagtgact ggcatcgcga ctggtaaaca gcacaacacc
     4561 caaatcagca tcgcttccca gcatcgacat cgccaccgat cccaccgctt tcgcttccgg
     4621 tagcaccacc agcagctccg gtccgttaag cggcccaaga tagtgctgtt cctgccccaa
     4681 acgctgaata cgcagcggtt cgaaagactg acggcttaat gccagatgag tgtggttcga
     4741 cggcgcacct aagcgccagc gatctggaaa caggcgcaga ctcgcaccag ctaggccgag
     4801 atcgcgcgcc cagcggtgaa agcgcatcag catatcgtcg agactgctgg cggcggtgag
     4861 actgcgctgc aggtagagta ggcgataaaa cagcccttcg ttggcgatag cctgctccat
     4921 caacaacgcc atgttctctt ccagaacatg aatgtgatta cgtgcgcggg ccatgtgcca
     4981 ctcgaccaac gaaacggtgc cgcgtaccgg atgcggcaca cgtatcgctt ctactgcgcg
     5041 cgcattgcgg ataaaaaact caggattttt aatcagataa tcgacaaccg cccggtcatc
     5101 aagctccgtg agtgtttcct gcagttcttc ccctggttgc ttcatagatg aataaatcca
     5161 tcgtagacat gtaccgccgg gccagtcata tataacgggt gacccggacc tttccaggcg
     5221 atatcaagac gaccgccggg gagttccacg cgtacttctt cgtccagcaa accttgctga
     5281 atccctaccg ccaccgccgc acacgcgccg ctgccgcagg cctgggtttc tcctgcccca
     5341 cgctcataaa cgcgtaaacg aatatgctcg cgcttaacca cttgcataaa accgatattg
     5401 gcgcgctccg gaaaacgctc gtgactttcc agaacaggac caagcgtttc taccgccgcg
     5461 gtatcgacat catcgacctg aatcacgcaa tgcggatttc ccatcgacac cacgccgcat
     5521 aagattgtct gctcggcggc gcgcataata taggtctttt ccgctttgtt agcgcgaaac
     5581 ggcacggcgg aaggttcgaa gttgggttcg cccatattta cgcggaccag atcatcatcg
     5641 gtgacggtca gaaccatccg cccgttggcg gtgctgacgc ggatatcacg cttattggtc
     5701 agtcctttca gacgcacaaa acgggcaaag cagcgcgcac cgttaccgca ctgcgccact
     5761 tcactgccat cagcattgaa aatgcgatag tgaaaatcca gttcaggatc atacggcggc
     5821 tcaaccacca gcagctggtc aaaccctacc cccaggtgcc gatccgccag gcgacgaatc
     5881 agctccggtg aaaaaaagac attctgcgtt accgcgtcga cgaccataaa atcgttgcca
     5941 aggccatgca ttttcgagaa ctgcatcatt tactccaatc acgcgggtac agaaactgac
     6001 ttttagtaat tcacctggga tggaccatcg ccagtggcgc gatcgttttt atccggcacc
     6061 gtggattgcg tttgcgtctc taccggtttg gtcggcggcg gtgcgttttt atctgcaggc
     6121 gggaaataga gcggaccttt cagaccgcag cccgtcaggc tgaagagagt aagtaataca
     6181 gtgagtgcct taaacacgtt tttcattatg gattgcctgt aaagttcatc gctttctgct
     6241 ttctatcatc gcaggagaag gcgtaaaagc aagcgtttag cgactcttta tgaagtctga
     6301 aaagtgagga aatgctgcga gtataacgct tactcgtgag gatttatgtt ctgctggcag
     6361 tttcgtgatc aacacctcgc gccaggatgg ctttatgcac tatcgcaatg tggtttgttt
     6421 gttgtcttgt tcattgtttt tatcgtcggc atggggatgt cggctttccg cacctgaaca
     6481 taatatttac cagaaacagg gaaaaggcgt ggtgtacctg cgcccttata aagaaacaaa
     6541 tctttctcta cctgacgtga attacaagag tttgcgtcgg ttacccaatt tattaataga
     6601 cccgacaaca ctagacgaat gggataaaga accgcctctg actgatttga caaccgacta
     6661 cctctatgag ggcgcacagg cctggtatcc tcattattca tggcatagcg atggtcgcaa
     6721 tatcctctac gcgggtgaag ttgtgcaaaa ccctcccggc acacctcccg tcgatgtcgc
     6781 ctcgttcaag gcatggggtg attttgccgc agataaacac agtctctatt ttgaaggcaa
     6841 gcgcaccgat gacaatggtg gtggaaatag cttggatatc aaaacgctgc atcaggtgga
     6901 attccggcct ccctgggatc ctgacttact ggggctgata ttacgtgacg ctaatttctt
     6961 gtatatcaat gggcatcgcc tcgctgaccc cgagagtttc cgtgtgctgg cgcagaagtc
     7021 ctgggatcag cgaggaaagt tttccaccac gtttaacccc tgcattgccg taccatttgg
     7081 cccctgggat accctggctc gtacacggac caaaatcctg ctcaacggcg aacagcttga
     7141 tgccgacccg gacacctttt ccgtcgtgcg ctggatgccc ggctcactct tgacctggcg
     7201 tgataaaaac gggctgcagc gtaaagtcct cgacaaggaa aatctggcgt gggatgaaga
     7261 tttaacaaag cactgtctgg atttttctct gctggaaaag aaagtgttct ggcgtaaagg
     7321 acctgcttgt aaacaggaag aattacccgg actcgatccg gagcagtttc aacccatcag
     7381 tgatgctgtc gcccagcatc aggactcgct ttataccatc atcgaaacag agtctggtaa
     7441 ccgtaagctg gagatcgtga aacttgatga tcccaatctt attatcaaca aacgtttcaa
     7501 cgccgggaaa cgccacggct atttacttac gcgtgccgaa gggtagccat accattccgg
     7561 tttacacgtg tttgaatctg acggaccgct gatcttactg gataaccgtt ctccggatga
     7621 acgcgaagcc catcttaatg accatccctt tttgcgcaga tggtatgccc gcgataaccg
     7681 ctacatttac agctttgatg gcgcgcagct ctggcgatac cgcaccgctg atccgaaaca
     7741 agttcgctta atctggaagg aacaacattc gggatatgtc tatggcgtaa attacaaaac
     7801 gggatatctg gacggaaaaa ttactgatga cggcgaattt attcctgccc cgcgcaatga
     7861 ggcgacaaaa tgaagggtca aataatcaca gttgcactca tcctgttggg acacacattt
     7921 ctgacgcctg ctgtacaggc catagggttc gactattaca atgatcacgg tgttatgtct
     7981 tacggtaaag gttattgggg ggataagaaa atcattgccc agtttccgga gatgagcagg
     8041 gccggtttgc gccttgtgaa aaatatctct gggaaaacgg gttatatacc ttccgatgaa
     8101 ataagcatag aaaatgagga ataccactgg gtgacagacg gccgcgttat tctctggcgt
     8161 ggcaaaatcg ttagcaatcc accgggaaca ctcactgtcg atattgccag ctttcaggct
     8221 atgggccgct tcgcggtcga taaatatagc ctctattttg acggacagcg caccgaaagt
     8281 aatagcggcg cgtcacgtgt tgagctggcg acactaaaag ctatcgaagg taactctacc
     8341 acgctggtgg atagtaaaaa tctctacttg tccggtcgac gtcaggggag cagcagtgat
     8401 gttactgtat tagaaaaaag atggtggggt attaatccac gtcttatgag tgtaaacaga
     8461 aattcatatt ccaatgatct gcttatccgc agtgggcaga atatttattt aaatggcgtt
     8521 caccttacgg cgaatgcaga ctcatttgag ataattcgct ggatacctca ttcactgctg
     8581 gtttttcgcg acaataaggg tctgcatcgt tatccctttg gtcaattatc aggcaaagcg
     8641 atacccgtag atgatgacgt ctcttttgaa gtaggggaaa gtcgcgttcg ctggcgtaaa
     8701 cagctcacgc ccgaccgtca gtggagcaag tggatagacc taccaggtat tgaacct
//
LOCUS       DAJTSA010000146         8632 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000146 DAJTSA010000000
VERSION     DAJTSA010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8632
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.146"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..104)
                     /locus_tag="OO369_003726"
     CDS             complement(<1..104)
                     /locus_tag="OO369_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCR5983846.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(145..600)
                     /gene="csgB"
                     /locus_tag="OO369_003727"
     CDS             complement(145..600)
                     /gene="csgB"
                     /locus_tag="OO369_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR5983847.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
     gene            1354..2004
                     /gene="csgD"
                     /locus_tag="OO369_003728"
     CDS             1354..2004
                     /gene="csgD"
                     /locus_tag="OO369_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR5983848.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2009..2398
                     /gene="csgE"
                     /locus_tag="OO369_003729"
     CDS             2009..2398
                     /gene="csgE"
                     /locus_tag="OO369_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR5983849.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            2423..2839
                     /gene="csgF"
                     /locus_tag="OO369_003730"
     CDS             2423..2839
                     /gene="csgF"
                     /locus_tag="OO369_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR5983850.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2866..3572
                     /locus_tag="OO369_003731"
                     /pseudo
     CDS             2866..3572
                     /locus_tag="OO369_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3705..4196)
                     /locus_tag="OO369_003732"
     CDS             complement(3705..4196)
                     /locus_tag="OO369_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR5983851.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4298..4852)
                     /gene="ycdY"
                     /locus_tag="OO369_003733"
     CDS             complement(4298..4852)
                     /gene="ycdY"
                     /locus_tag="OO369_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR5983852.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4876..5613)
                     /gene="ycdX"
                     /locus_tag="OO369_003734"
     CDS             complement(4876..5613)
                     /gene="ycdX"
                     /locus_tag="OO369_003734"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR5983853.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5668..6606)
                     /gene="ghrA"
                     /locus_tag="OO369_003735"
                     /pseudo
     CDS             complement(5668..6606)
                     /gene="ghrA"
                     /locus_tag="OO369_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6841..6928
                     /locus_tag="OO369_003736"
     tRNA            6841..6928
                     /locus_tag="OO369_003736"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6875..6877,aa:Ser,seq:gga)
     gene            complement(7121..8107)
                     /locus_tag="OO369_003737"
     CDS             complement(7121..8107)
                     /locus_tag="OO369_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983854.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(8104..8632)
                     /locus_tag="OO369_003738"
                     /pseudo
     CDS             complement(8104..8632)
                     /locus_tag="OO369_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         2458 a         2076 c         1841 g         2257 t
ORIGIN      
        1 ccaccaccgt ggttaccgcc gccgccgtac tgaggaacaa cacctgccag agcgctaccg
       61 gagaatacga ttgctgcaat tgctgctact tttaaaagtt tcatgtaaaa cccccatcgg
      121 attgatttaa aagtcgaatg gtaattaacg ttgtgtcacg cgaattgcca tttgcgactg
      181 tctctgcact acaactgccg ttttttgagt accatactgt gtaatatttg ctttattacc
      241 agaacctttc tggataatca tcgcagtatt accataagca ccttgcgaaa tactggcatc
      301 gttggcactg cccgcctgat caatatatgc aaggttataa tctcctgtct ggtcaatctt
      361 tgcccgattg ctactacctt cttgcgcaac aaccgccaaa agttttgagc ctccctgccg
      421 taactgagca ctattattag tcccagcctg accaattatg gctgcctgat taaatgaaga
      481 cttactcaat tcatttaccg cgaagttata ttctgaatta gctaaatcat aacctgctgc
      541 ggctgcaatc ccaggcgcac ccagtattgt taacatcata aataacaatt tgtttttcat
      601 gttgtcaccc tggacctggt cgtacattta agaaattaaa tcatttcaac ttggttgtta
      661 acgcaacctg tattttgtta acgctgcgtt acgatggaaa gtatgtctgc ggaaatattt
      721 ttaataactc acccgcgcgt tgtatttttc tttttttgtt cataccaaag tattaaaacc
      781 cgcgtaaata cgctgttatc tacgcaaaaa atattttgtt ttctcttaaa tctccgtttt
      841 ccgctatcaa aaatcaccag acagtcattc ttcttgcccg tcgctgattg ctgcgatatg
      901 tcttgcgcac aagccgtgac aaacctcgtc tttttgcagt aaaaaaattg tccacggagg
      961 tgtggagaaa aaacaaaaac gttttacatg acgaaaggac tacaccgaaa tattttttat
     1021 atgtattatt agtaagttag caccatttgt atgatttttt aaaattgtgc aataaaaacc
     1081 aaatgtacaa cttttctatc atttctaaac ttaataaaac cttaatgtta acattttaat
     1141 ataatgagtt acattttgtt acatgtttaa cacttgattt aagatttgta atggctagat
     1201 tgaaatcaga tgtaatccat tagttttata ttttacccat ttagggctga tttattacta
     1261 cacacagcag tgcaacatct gtcagtactt ctggtgcttc tattttagag gcagctgtca
     1321 ggtgtgcgat caataaaaaa gtggggtttc atcatgttta atgaagtcca tagtattcat
     1381 ggtcatacat tattgttgat cactaaacct tctttgcagg cgacagctct cttgcagcac
     1441 cttaaacaat cgctagcaat tacaggaaaa ttacataata ttcaacgttc tctggacgat
     1501 atctcttcag gctctattat tcttctggat atgatggaag cggataaaaa gcttatccat
     1561 tattggcagg atactttgag caggaaaaac aacaatatca aaatattgtt gctaaatacg
     1621 cctgaagatt acccgtaccg cgacattgaa aactggcctc atatcaacgg cgttttttat
     1681 gccatggagg atcaagaacg tgttgtcaat gggttgcaag gcgtcctgcg tggcgaatgc
     1741 tactttacgc aaaagcttgc cagttacctg attacacatt ctggtaacta tcgttataac
     1801 agcacggaat cagccctcct tactcatcgg gaaaaagaga tcctgaataa gctgcgtatc
     1861 ggcgcgtcta ataacgagat cgctcgttcg ttgttcatca gcgaaaatac ggttaaaacg
     1921 catctttata atcttttcaa gaagatagcc gtaaaaaacc ggacacaagc ggtttcctgg
     1981 gcaaacgata acctcaggcg ataaagccat gaaacgttat ttacgctgga ttgtggcggc
     2041 agaatttctg ttcgccgcag ggaatcttca cgccgttgag gtagaagtcc cgggattgtt
     2101 aactgaccat actgtttcat ctattggcca tgatttttac cgagccttta gtgataaatg
     2161 ggaaagtgac tatacgggta acttaacgat taatgaaagg cccagtgcac gatggggaag
     2221 ctggatcaca ataacggtca atcaggatgt tattttccag acttttttat tcccgttgaa
     2281 aagagactcc gagaaaactg tcgtctttgc actgattcaa actgaagaag cactaaatcg
     2341 tcgccagata aatcaggcgt tattaagtac ggacgatttg gcgcatgatg aattctaaat
     2401 aaaaaattgt tcggaggctg caatgcgtgt caaacatgca gtagttctac tcatgcttat
     2461 ttcgccatta agttgggctg gaaccatgac tttccagttc cgtaatccaa actttggtgg
     2521 taacccaaat aatggcgctt ttttattaaa tagcgctcag gcccaaaact cttataaaga
     2581 tccgagctat aacgatgact ttggtattga aacaccctca gcgttagata actttactca
     2641 ggccatccag tcacaaattt taggtgggct actgtcgaat attaataccg gtaaaccggg
     2701 ccgcatggtg accaacgatt atattgttga tattgctaac cgcgatggtc aattgcagtt
     2761 gaacgtgaca gatcgtaaaa ccggacaaac ctcgaccatc caggtttcgg gtttacaaaa
     2821 taactcaacc gatttttaag ccccagcttc ataaggaaaa taatcatgca gcgcttattt
     2881 cttttggttg ccgtcatgtt actgagcgga tgcttaaccg ccccgcctaa agaagccgcc
     2941 agaccgacat taatgcctcg tgctcagagc tacaaagatt tgacccatct gccagcgccg
     3001 acgggtaaga tctttgtttc ggtatacaac attcaggacg aaaccgggca atttaaaccc
     3061 tacccggcaa gtaacttctc cactgctgtt ccgcaaagcg ccagggctta caaaacctgc
     3121 ttaacgagcg caaggttatt cgtgcggcac aagaaaacgg cacggttgcc attaataacc
     3181 gaatcccgct gcaatcttta acggcggcaa atattatggt tgaaggttcg attatcggtt
     3241 atgaaagcaa cgttaaatcg ggcggtgttg gggcaagata ttttgggatc ggtgcagaca
     3301 cgcaatacca gctcgatcag attgccgtga acctgcgtgt cgtcaatgtg agtaccggtg
     3361 agatcctctc ctcggtgaac accagtaaga cgatactttc ctatgaagtt caggccgggg
     3421 ttttccgctt tattgactac cagcgcttgc tggaagggga agtgggttac acctcaaacg
     3481 aacccgttat gctgtgtctg atgtcggcta tcgaaacagg ggtcattttc ctgattaatg
     3541 atggtatcga ccgtggtctg tgggatttgt aaaataaagc agaacggcag aatgacattc
     3601 tggtgaaata ccgccatatg tcggttccac cggaatcctg acagataaaa atgccggaat
     3661 taataatccg gcatttactg tttaagcgaa agcgtgagtg cccctcacgc tttatttttg
     3721 atttcctgct cacggtgagc cgtctttgca ctacgcgccg ccagccacag gccgctgttt
     3781 ttcattgcgt aaccaaatac cagcccaagc gccagcgaag gtaacaccag tttccagtca
     3841 ccctgcccgg caaacgtcgc gcatgcgcct ataaacgtcc ccggaacaaa tgaaagcagc
     3901 agctgtttgg cctgaataca catcagaaac gcgacaatac cggttatgac ataaccgaga
     3961 atttccagat gtggtgccag tgcactaccg taaataatga ccatcgccca caccacgcca
     4021 ctaagcaggg ttgcagcgga gatcgccagc cctttcagcc caccttgcgg gcaagcaaag
     4081 taggccgtac agcccaggaa gcccgcccag cttagtaagc ccagggaaac agccacccat
     4141 ccccagatac cggagagaat gcctgttgtg attgcaatag agagaagtat gttcatgccg
     4201 cgcatgatag cagaaaacgc ggccatgaat gagatcgagc acacatttta tgcaacaaca
     4261 aattatctgt tgcagttatg agtgacgatg atcacattta ctcttcagaa tcttcttcca
     4321 gctcgtccca cattgcacta atggcatcac gggttagcgg tgccatggtg cgccagaaag
     4381 gcgtggttgc atgggcctcc actttgccga ggaacgcacc acaccagggt aacagatact
     4441 cactgaacag tgtttccagt gcttcgctct catcttccgt tgactgatct tccagccagg
     4501 aagccgcaag cagcaatgtg ccgatgtgat cggctggcgt atccgccagt agcatccctc
     4561 gttcggaaag aaaagcgcgc acttccgctt ccgttgcgtc ctcaacccat gcgctacgat
     4621 atggcggcac ggcacattca tcgccgataa acaacgcatt gtaatcggca gagacttgcg
     4681 tcatatcaca gcttttctgt aaacgcgtca gtaactcatc ctgctccagt ggccagttcg
     4741 cagccagttt cccctcacga atcagagtaa acagcggcac cagtaaagga tcttgcggtt
     4801 ggcggtaata gagcgaaccc agcacacgac agaggataga aaactcgttc attaatatat
     4861 ttccattact agtaattaaa ggtctgcaaa ttccgcaatc ggtgccatac cgcgagattc
     4921 aaggaagttc agtaagcggc gcggagaaac attcaaaatg cgctctagcg gaaaatctac
     4981 cgcgtcgagg attttaagac actcttcaaa ttcccccatg gtaaacgcgg tgtgagaatc
     5041 cgagcctaat gccacccaac ctccagcatc acgtaccgct gcagctaccg cacgacagtt
     5101 gtcttcactg cccttacgtg agtgtaaaaa tgaggaatta ttgatttcca gcgccacctg
     5161 atgtttcgcg gctgcttcag caacggcttt aacatctatt tcatatttcg gatttccggg
     5221 atggcttatt atatgcacat tgccgctggc gatagtggag atcatcgctt gtgtattggt
     5281 cgctttgtca tgtggcgcaa aaaccggctc atgaaaaccg gcaataatca gatccagcga
     5341 gtcaaacatt ttaccgctgc agtcaatttc accatcaaca tttttaatgt tagcttcaat
     5401 gccgcgcagg atccctaccc catcaaccac tcgcggccag atacgcatgt taatgaagtg
     5461 ccagtgatgc ggcgcatctt ccatatccgg gccatgatcg gtgatcgcaa aaagtttaat
     5521 gcccttttgt ttggcctggg caatgtaatc actcaatgtg ctatatgcat gtgtgctggc
     5581 aacggtatgc atatgaaggt cgacgggata cataactctc tcctgtattc aattcccgcc
     5641 aaggatagca ggaatcctga tgctttatta gtagccacgt gcgcggtcga cttgcccgca
     5701 gaccctctcc cctttttcga gctgggcaat agtgcgagaa atgtactcca cagcttcagc
     5761 gggacgggta atcgcggcga catgtggtgt tatcgtcacg cgtggatgtt gccagagcgg
     5821 actttcaggc ggtaagggtt cacgattaaa aacatccagc attgcgcctt taactttccc
     5881 gctatccagc gccgcgagca ggtcatcttc cacaacatga acaccacgcg ccaggttgag
     5941 gagatacgcg ccatcaggta atttttcgag taattgttga ttaataatgc cgacggtttc
     6001 aggggtattc ggtaacaaat taatcaatac ccgacattgg ctcagaaatg cagacagttc
     6061 ttcccgtccg gcaaagcttt gcacgccagg ccacgattta cgggttcgac tccagcaacg
     6121 cagcggaaag cgctaggttt gcagactctg agcaacttta cagcccagta cgcctgcgcc
     6181 caaaatgccg atggtaaaat cttcccgatg atattcaggc agcggttgcc aatgcgaact
     6241 attttgctgg atgcgataat cgtcaaaacg tcgaaaccaa tgcagcacct gactgacagc
     6301 atattcctgc atttgctcgc ccataccggt atcttccagg cgaaaaagtg gaacagaagg
     6361 gttcagcatt tcagggtgtg cctgtagctt gctcaaaata gaatcaacac cggccccgag
     6421 tgcgaacacc gctttaagat cgcgccctgc cagcatttca acaggaggat gccagactaa
     6481 aacataatca gcagagtcat tatctccgct tttccatgcc ctgactcttg cctgaggaat
     6541 agctttgcgc agtgcctcaa tccaccattg ggtatcgaac gttgggtgat aaaagatgat
     6601 atccatactg actcccgaaa agcgttgtgc gaaatttatt cgcacttatc gttatgatct
     6661 acaaaggcca ccagcataac aaatccgtgg tcggtggcaa aaaaagcaga tttcgcttat
     6721 taaaaccaca cattgattga atgtttgaat aaacgcgcga ttttttcaaa aagtttgttg
     6781 acctcaggtc atgatttccc taaattagcg cccgttccag caagacagga acgacaattt
     6841 ggtgaggtgt ccgagtggct gaaggagcac gcctggaaag tgtgtatacg gcaacgtatc
     6901 gggggttcga atccccccct caccgccata tttaaagaag agcgcgtacg aaagtacggg
     6961 cttttttttc gtatattgta cacaccgggt gcccccgccc acgtctgtta gggcgagggg
     7021 aaattgtgca catcaactgt ctggttactc agtcaacaat caaaatgcgt aagatagtac
     7081 agaacatata tgcgttatct ggtcacttcc tgtaacttcc tcacttatta aaatgccccc
     7141 aacggaaata catgtcacat gccatcataa aatcgttatg ccagaggaat cgacaatgga
     7201 aaaaatagta aattaagcac ggtacatagc agagtaaaaa ataggcttca aaccaaatcc
     7261 gccattccac aagcgttgcc aataccgaca ccagcgtaat ccacatcatt tcaataaccc
     7321 gcaatatgaa taaccgaagc caaagcggct tggcaaaaaa ggtcgacaac gtattttcac
     7381 tggagaaaaa cagatcgatt tcctgcaaaa aaaacctgcc cagaaaagcg ggccgcttgg
     7441 agaagcgcag aaaaaacaga ttaatcgcca acactacgcc acaaagaatg agcaatgcaa
     7501 ttatcgattt aaacgttaac cactgcccct ctgtcacaaa agaatcactg ttacgcagcg
     7561 ggctgatagt aagcaggtta acaaccgttg agatgcacac cgaaagcagc acatagcgag
     7621 tcagactttt atattttact tttttcgcta ctgaaatagc gacctgcttg acataactgg
     7681 tgcggatcat ccgaaacagc agaaagatga ccaacggccc aagtaacgaa atcaggaaca
     7741 gcgcatataa cgagggtaga agctggtata gcccccaact cattgcactc aacatgatta
     7801 acgacagaaa tcctgccacc agaggttgtg taagaaataa ttctattttg tggttctgct
     7861 ttttcttaaa ccacgaaaaa tcgatattct tcgtgacgca tttataagcc cagtacgcct
     7921 tgagatcaaa aataaaatta tacagcacca gggccagaat cccactaact acaccaatat
     7981 cctctgatgg aaaagtaaac cctgcatttt tccaataaaa gcctatcaga ataagtccaa
     8041 tagcataaat gtatatcaga tttcgtttat cctgaaagcg aataaaagtt aaataatcag
     8101 gaatcatttg aatacctttg catcactaaa tgctttacga caatgcataa ctcgttgctg
     8161 atccacgcca gcaaaatcat ttaacatatt ccccaaatag ggcttattac ttatttcaag
     8221 gaaagctacc ggggttggca tattcagaat atgtatcgag agattcagtc catttcaccg
     8281 tatgagttaa gtgtaatgag agattttctt tgataattga cggagcgacc tcgctttttg
     8341 ctgttacgtt catcagaacc tgatgctctg gcgaagcgat atccagtccg gcaagataat
     8401 cgcgcattgc ctgaacgccg tcttccatca aacgcgtgtg ccaggcaccg ctcacaccga
     8461 gtttaaccgg ttcgtatcca gcggccatca gcagcgtggt aaattcattc aacgcagcct
     8521 gcgttccccc aataacctgc tggcgcggcg tgttatcaca gctaatatcc agcgcaatgc
     8581 ctgattccgt aatcatcgtc tgcagttgtt cgcggttgat gcctttaacc gc
//
LOCUS       DAJTSA010000147         8373 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000147 DAJTSA010000000
VERSION     DAJTSA010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.147"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..956
                     /gene="espL1"
                     /locus_tag="OO369_003739"
                     /pseudo
     CDS             1..956
                     /gene="espL1"
                     /locus_tag="OO369_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1128..1235
                     /locus_tag="OO369_003740"
     CDS             1128..1235
                     /locus_tag="OO369_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983855.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1288..2046)
                     /locus_tag="OO369_003741"
     CDS             complement(1288..2046)
                     /locus_tag="OO369_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR5983856.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2333..3262
                     /gene="pfkB"
                     /locus_tag="OO369_003742"
     CDS             2333..3262
                     /gene="pfkB"
                     /locus_tag="OO369_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR5983857.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3363..3653
                     /gene="ghoS"
                     /locus_tag="OO369_003743"
     CDS             3363..3653
                     /gene="ghoS"
                     /locus_tag="OO369_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR5983858.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3759..4619
                     /locus_tag="OO369_003744"
     CDS             3759..4619
                     /locus_tag="OO369_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR5983859.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4660..5196)
                     /locus_tag="OO369_003745"
     CDS             complement(4660..5196)
                     /locus_tag="OO369_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR5983860.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5343..6011
                     /gene="hxpB"
                     /locus_tag="OO369_003746"
     CDS             5343..6011
                     /gene="hxpB"
                     /locus_tag="OO369_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR5983861.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6174..6764
                     /locus_tag="OO369_003747"
     CDS             6174..6764
                     /locus_tag="OO369_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5983862.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6897..8287
                     /gene="tcyP"
                     /locus_tag="OO369_003748"
                     /pseudo
     CDS             6897..8287
                     /gene="tcyP"
                     /locus_tag="OO369_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
BASE COUNT         2173 a         2003 c         2007 g         2190 t
ORIGIN      
        1 tgctcagacc tgggctgata ggtatgttcg caactgtgca attgctattc tgcccctatt
       61 acctgtggga gttctagggg ctattttcgc gggtattgca tcacgaatgc catttgcccc
      121 tatacatcca tcggcaatgt tgttaataat ggctacaggc cagactcaac agcttattac
      181 attattcaaa cagttgccca tactccctga aaaagaaatc attgaaataa taactgcgca
      241 gaatagcgtt ggtacacctg ctttattttt ggctatgatg aacggacata ctgacaacgt
      301 gaaaatattt atgcaagaaa ttcagtcact ggtagataat cacatcattc atgaagataa
      361 tctggttaaa ttactgcaaa ctaaaagtgc taacgaaaca cctggacttt atatctccat
      421 gttgtatgga ttcgatgaaa taatcgatat ctttctgaat acattaacca ctccaatagc
      481 actaagagct tttaaacaaa aaactggtga tgagtatttt agccatgaaa atacatgatg
      541 gtgagccagg attatacgcc gcaatggaaa ataatcaccc tttgtgtgtc acacggttcc
      601 tctctaaaat taatggcatc gcctttaaat acaagttgag caaagctaac atcatggatt
      661 tattaaaagg agctacagca cagggaaccc ctgcattata catcgccatg agcaagggta
      721 atgaagacgt cgtgttatct tatatatcga cgctgggtgc ttttgcaaaa aaacattctt
      781 ttagtcaaca tcagttattt acactattgg acgctaaaaa tcatgacaac atgtcagctg
      841 ttcatatagc cattcatcat aagcattata aaactgtaga aacatattat gctgctataa
      901 atgtcatcag ccaaagcctg agttttagtg ctgatgaaat aaagacgtat ttataaccag
      961 ctatttacga tatatatcga tacataattt tatttttttc ataaaacgca gaaacagtga
     1021 ccatgtgcac aacgccaccc gacggctgta ataacaaaaa atcccttgat gcctgtccct
     1081 tttgttacac tccgttatca cgcacaagag atatgcagga cactggtatg ccgactaaac
     1141 gctttgataa aaaacactgg aagatggtgg tggtgctact ggcaatctgt ggcgctatgt
     1201 tgttgctacg ttgggcagca atgatttggg gctgagcagt gacaaacgaa gacagccaga
     1261 ccgcataaca cggtccggca gataatttta catgctgtag cccagcgata aggttgtccg
     1321 acggtcggta tgttccggtg cagactctgg aggttcagag ttccatgtga cgttgtaagc
     1381 caccttcagt ccaaaatgtt cattaatagc aacatttaat gcgctttcgg agttcagcgt
     1441 tgtgtcttcc gcgccaaaga ccgaaacacc ctgcgtaaat ttagcgttgt cagtcaactg
     1501 ccatgcataa gccccggagg catagcccag cggctgggtt tcactggcat tgtcggtata
     1561 tttgtcgtaa cgcacacctg gaccgaattc aaagcggaaa ctgtgtaccg ggccattcaa
     1621 aaactgacga ccataacccg cggtcaacac atcgcgctct cgatagccgt tataacggtc
     1681 tgtcagccag cttgcctgac caaataaata gtcataatca gttaaattaa agcggctacg
     1741 cccgcccgcc gcatattttt ctgaagaacg ctcatcatta gaagaagtat tactggcgtt
     1801 cccccacagc gaccaggcag tggtttgtcc ataccaggtc atggtggtgt cagccgtaag
     1861 tgaggagctt ttcgtattgc ctgattgtgc aagatatcct gcgttcagat taccttcgaa
     1921 aggtttttta gcgctggcag gatcgtccat gacagtaaaa acggaatcat cggcagctgc
     1981 attcagtgac gcaaacatgc cccccgccaa cataacgatg gcaggaactg tcttcaaaag
     2041 cttcatttat caagagtccg tacaacaaaa aaagagacca tcgcggtccc ggaaactttc
     2101 ttaaggatca aagattagcg tccctggaaa ggtaacgaat tataaaaagg cgcgaataac
     2161 ttagcaatgt attcttattt cattttttga ataagcatgt ggcgaaaaca gatttttatt
     2221 tatatatatt tatctgcaaa attttaaata aagctctaat aaatcatatt gttaatttct
     2281 tcactttccg ctgattcggt gccagactga aatcagccta taggaggaaa tgatggtacg
     2341 tatctatacg ttgacacttg cgccctctct cgatagcgca acaattaccc cgcaaattta
     2401 tcccgaagga aaactgcgct gtaccgcacc ggtgttcgaa cccgggggcg gcggcatcaa
     2461 cgtcgcccgc gccattgccc atcttggagg cagtgccaca gcgatcttcc cggcgggtgg
     2521 cgcgaccggc gaacacctgg tttcactgtt ggcggatgaa aatgtccccg tcgctactgt
     2581 agaagccaaa gactggaccc ggcagaattt acacgtacat gtggaagcaa gcggtgaaca
     2641 gtatcgtttt gttatgccag gcgcggcatt aaatgaagat gagtttcgcc agcttgaaga
     2701 gcaagttctg gaaattgaat ccggggccat cctggtcata agcggaagcc tgccgccagg
     2761 tgtgaagctg gaaaaattaa cccaactgat ttccgctgcg caaaaccaag ggatccgctg
     2821 catcatcgac agttctggcg aagcgttaag tgcagcactg gcgattggta acatcgagtt
     2881 ggttaagcct aaccaaaaag aactcagtgc gctggtgaat cgcgaactca cccagccaga
     2941 cgatgtccgc aaagccgcgc aggaaatcgt taatagcggc aaggccaaac gggttgtcgt
     3001 ttcccttggt ccacaaggag cgctgggtgt cgatagtgaa aactgcattc aggtggtgcc
     3061 accaccggtg aaaagccaga gtaccgttgg cgctggtgac agcatggtcg gcgcgatgac
     3121 actgaaactg gcagaaaatg cctctcttga agagatggtc cgttttggcg tagctgcggg
     3181 gagtgcagcc acactcaatc agggaacacg tctgtgctcc catgacgata cgcaaaaaat
     3241 ttacgcttac ctttcccgct aacaaaaaca ttcccccagc attgggggaa tcaccaccaa
     3301 cctgtcggca acgcgtttct ccgactatgc tcaaaagtca tgtgataaca aaggggtgaa
     3361 ctatggccag tggcgatctt gtccattatg tcataaccgt catgttgcat gaggatacat
     3421 tgactgaaat taacgagttg aataattacc tgactcgcga cggttttttg ctcaccatga
     3481 cggatgatga gggaaatatc catgagctgg ggactaacac ttttggactt atcagtaccc
     3541 aaagtgaaga agaaattaga gaactggttt cggggcttac ccaaagtgca accggcaaag
     3601 atcctgaaat caccatcacg acctgggagg aatggaatag caacagaaaa taaatggttt
     3661 ttgggcaata atcagtctgt ggtgtgcgtt agctcgtgtt tttacaccgc attcttgcgc
     3721 taaccttatg atctggcaga caacatggga gagacatcat gtggcaggca atcagtcgtc
     3781 ttttgagcga gcagttaggt gaaggcgaaa tcgaactgcg taatgaactg cctggcggag
     3841 aagtccatgc cgcatggcat ttgcgctatg caggacatga ctttttcgtc aaatgtgatg
     3901 aaagggaact gcttcccggt tttaccgccg aagccgacca actggagtta ctgtcgcgta
     3961 gtaaaaccgt caccgtgcct aaggtttggg cagtaggcgc tgaccgtgac tacagttttc
     4021 tggtgatgga ttatctccca cctcgtccgc tggatgcgca tagcgcattt attcttggtc
     4081 agcaaattgc gcgtttacat caatggagtg accaaccaca atttggcctc gattttgata
     4141 actcgctctc cacaactcca cagcccaaca cctggcaacg tcgctggtca acgttttttg
     4201 ctgaacaacg gattggctgg cagttggaac tggcagcaga gaaagggatc gctttcggca
     4261 atatcgacgc catcgtcgag catattcagc agcgtctggc ctcgcatcaa ccgcagcctt
     4321 ctctgttgca cggcgattta tggtccggca actgtgcgct gggtccggat ggcccgtaca
     4381 ttttcgaccc tgcctgcgac tggggtgacc gagagtgcga cctagcgatg ttaccgctgc
     4441 atactgaaca gccgccacaa atctatgacg gctatcagtc agtatccccg ctacctgccg
     4501 atttccttga acgtcaaccg gtttaccaac tctacacgct gttaaatcgt gcaagattat
     4561 ttggcggtca gcatttggtt attgctcagc agtcattgga tagattatta gcagcatgat
     4621 atgggttgag gataatggcc gctccgtgcg gccttttgat taaataaacc caagcagaga
     4681 aaagaacaca taacctgcag caatacaaat aaccggcagg atatatagtg agaaaaactg
     4741 caggaaaata gtatggtgcg gaacaacaat acgggactca atttgttcac gcgttagccc
     4801 ctcttcccct ttggcttttt ccagaatgag ttgatcttca acaccttccc gcaagaagcg
     4861 cgcctggcga ctcatccggg caccggaatc ctgcaacgcc agtccgacaa aaatcaggat
     4921 gaaaatcacc cagaacataa cgttcacacc accattaaaa ttaggggtcg gggagttata
     4981 ccagaacaga ttcaaaaaag gcgtattcgc ctgcatcata tcgatcatga catgagcaaa
     5041 gtcgagcatg accgcattaa tgccttcctg gttttcctgc cgggtattca taaatttcag
     5101 caaagaaatc aatgtagaga tcaatgcagg tataaaaatc acccaaccca aaatcctttt
     5161 caaaacagca atgcgtccag cttgttgata cgtcatgagt tctccttgat taagacgcgt
     5221 cgtttcactt agtttacctg tagatatctg ttttcgccca ttctttaaag gcgatatgat
     5281 aggcgcttaa tcataagcac ggcttaatac cttacacata atgctctaaa ggagaggttg
     5341 taatgtcaac cccgcgtcag attcttgctg caatttttga tatggatgga ttacttatcg
     5401 actcagaacc tttatgggat cgagccgaac tggatgtgat ggcaagcctg ggggtagata
     5461 tctcccgtcg taacgagctg ccggacacct taggtttacg catcgatatg gtggtcgatc
     5521 tttggtacgc ccggcaaccg tggaatgggc caagccgtca ggaagtagta gaacgggtta
     5581 ttgcccgtgc catttcactg gttgaagaga cacgtccatt attaccaggc gtgcgcgaag
     5641 ctgttgcgtt atgcaaagaa caaggtttat tggtgggact ggcctccgcg tcaccactac
     5701 atatgctgga aaaagtgttg accatgtttg acttacgcga cagtttcgat gccctcgcct
     5761 cggctgaaaa actgccttac agcaagccgc atccgcaagt atatctcgac tgcgcagcaa
     5821 aactgggcgt tgacccactg acctgcgtgg cgctggaaga ctcggtaaat ggcatgatcg
     5881 cttctaaagc agcccgcatg cgttccatcg tcgttcctgc gccagaagcg caaaatgatc
     5941 cacgttttgt attagcaaac gtcaaactct catcgctgac agaactcacc gcaaaagacc
     6001 ttctcggtta atgaccaggg gcagtgatca cgctgcccct ggttctttat ctgaatttgc
     6061 attcaactga cggattaatc gtcaatttaa gagaaagagt tacaccgtca ccacttccgt
     6121 gcactgtata aaaatcctat actgtacgta tcgacagttt agtgagtttt atcatgacgg
     6181 cggaaggtca ccttctcttt tctattgctt gtgcggtatt tgccaaaaat gccgagctga
     6241 cgcccgtgct ggcacagggt gactggtggc atattgtccc ttccgcaatc ctgacgtgtt
     6301 tgttaccgga catcgatcac ccaaagtcgt ttcttgggca gcgattaaaa tggatatcaa
     6361 aaccgatcgc ccgcgctttt gggcatcgtg gttttaccca cagtctgctg gcggtatttg
     6421 cgctgctggc aaccttttac cttaaggttc cggaaggctg gttcattccg gctgatgcgc
     6481 tacaaggaat ggtgctgggt tatttgagcc acatacttgc cgatatgctg acacccgccg
     6541 gtgttcccct gctctggcca tgccgctggc gtttccgctt gcctatcctg gttccccaaa
     6601 agggcaacca actggaacgt tttatctgca tggcattatt tgtctggtcg gtatggatgc
     6661 cccattcatt acccgagaac agcgctgttc gttggtcatc gcaaatgatc aataccttgc
     6721 agatccagtt tcatcggctt attaagcatc aggttgaata ctaaaaaggc aaaaatcacc
     6781 tttctggaat aagcaattcc atttgaatat aagagccagc tcacagttct gttaatcttg
     6841 cgccaacact atgactgcta cgcagtgata gaaataataa gatcaggaga acggggatga
     6901 actttccatt aattgcgaac atcgtggtgt tcgttgtact gctgtttgcg ctggctcaga
     6961 cccgccataa acagtggagt ctggcgaaaa aagtgctggt gggtctggtg atgggtgtgg
     7021 tttttggcct tgccctgcat accatttatg gttctgacag ccaggtactt aaagattctg
     7081 tacagtggtt taacatcgtt ggtaacggct atgttcaact gctgcaaatg atcgttatgc
     7141 cgttagtctt cgcctctatt ctgagcgcgg ttgcccgtct gcataacgca tctcagttag
     7201 gcaaaatcag ttttctgacc atcggtacgc ttttgtttac cacgctgatt gcggcgctgg
     7261 tcggtgtgct ggtcaccaac ctgtttggtt tgacggctga aggtctggtt cagggtggtg
     7321 cagaaactgc acgtctgaac gccattgaaa gtaactatgt tggtaaagtc tctgatcaga
     7381 gcgttccgca gctggtcttg tcctttatcc cgaaaaaccc gtttgccgat cttaccggag
     7441 ccaatccgac gtcaattatc agcgtggtaa tttttgccgc attcctcggc gtagctgcat
     7501 tgaaactgct gaaggatgat gcgccgaaag gggaacgcgt cttaaccgct atcgataccc
     7561 tacaaagctg ggtgatgaaa ctggttcgcc ttgtcatgca gttgacccct tacggcgttc
     7621 tggctctaat gaccaaagtg gttgcaggtt ctaacttgca agacatcatc aaactgggaa
     7681 gtttcgttgt cgcgtcctac ctcggtctgc tgattatgtt tgctgtgcat ggcattctgc
     7741 tgggcattaa tggcgtgagt ccgctgaagt acttccgtaa ggtatggcct gtgctgacgt
     7801 ttgcctttac cagccgttcc agtgctgcgt ctatcccact gaatgtggaa gcacaaacgc
     7861 gtcgtctggg cgttcctgaa tccatcgcca gtttcgccgc ctctttcggt gcaaccattg
     7921 gtcagaacgg ctgcgccggt ttgtatccgg caatgctgcg gtgatggttg cgcctacggt
     7981 tggcattaac ccgctggacc cgatgtggat tgcgacgctg gtcggtattg ttaccgttag
     8041 ttccgcaggc gttgccggtg tcggtggtgg tgcaactttc gccgcactga ttgtgctgcc
     8101 tgcgatgggc ctgccagtaa ccctggtggc gctgttaatc tccgttgaac cgcttatcga
     8161 catgggccgt acggcgctaa acgttagtgg ctcgatgaca gctggcacgc tgaccagcca
     8221 gtggctgaag caaaccgata aagccattct ggatagcgaa gacgacgccg aactggcaca
     8281 ccgttaattc ttatgctggc aacggtccgt tttgtatagg ggccgttgct ttacttttta
     8341 aatgttccat cgcggcgatg catactgact cat
//
LOCUS       DAJTSA010000148         8330 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000148 DAJTSA010000000
VERSION     DAJTSA010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.148"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..157
                     /locus_tag="OO369_003749"
     CDS             <1..157
                     /locus_tag="OO369_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983863.1"
                     /translation="SESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGED
                     VEPGDDF"
     gene            complement(166..564)
                     /gene="vapC"
                     /locus_tag="OO369_003750"
     CDS             complement(166..564)
                     /gene="vapC"
                     /locus_tag="OO369_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR5983864.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(564..791)
                     /gene="vapB"
                     /locus_tag="OO369_003751"
     CDS             complement(564..791)
                     /gene="vapB"
                     /locus_tag="OO369_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR5983865.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            873..1244
                     /locus_tag="OO369_003752"
     CDS             873..1244
                     /locus_tag="OO369_003752"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR5983866.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEASAST"
     gene            1265..6126
                     /gene="traI"
                     /locus_tag="OO369_003753"
                     /pseudo
     CDS             1265..6126
                     /gene="traI"
                     /locus_tag="OO369_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6146..6892
                     /gene="traX"
                     /locus_tag="OO369_003754"
     CDS             6146..6892
                     /gene="traX"
                     /locus_tag="OO369_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR5983867.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6947..7507
                     /locus_tag="OO369_003755"
     CDS             6947..7507
                     /locus_tag="OO369_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR5983868.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7638..7850
                     /locus_tag="OO369_003756"
     CDS             7638..7850
                     /locus_tag="OO369_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR5983869.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8094..>8330
                     /locus_tag="OO369_003757"
     CDS             8094..>8330
                     /locus_tag="OO369_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5983870.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1912 a         2086 c         2671 g         1661 t
ORIGIN      
        1 cagtgaatcc ggtgaagtgg tggatatggc cgcttatgag gcatggcagc aggaaaatca
       61 tccggacacc tggcagcaga tgcagcgtcg tgaagaggtg aacatcaatg tgcaccggga
      121 acgcggggag gatgttgagc cgggagatga tttctgatga acaggtcagc tccagtcttc
      181 agttctcagg ccgcccacac gttcaaattc ccgggtgtta ttagtcacaa taatcagccc
      241 ccgactgcgg gcatgacctg cgatcatttg atcaaatggc ccgacagggc gtccctgacg
      301 ggcaagttct gctcttatct ggccggtgtg tgtggctgca gcagcgtcgt aatccagaac
      361 atcaatgcgg gaaacaaatc cctcgatcac agcgagatta cgttcaggca tctggctttt
      421 ttctgcacca tatatcagct ccatcagggt gaccgaactg atacacattt tcccctggtt
      481 caggttaaaa cgctccctga cgctggcggg tttgttcttt atcgtaaaaa tgcagatgtt
      541 ggtatcgagc ataaacttca gcatcagaat gactcccttt cttgcatgcc gggctgttcc
      601 ctgttatcca taaaatcggt gctgacgctg tgtccgtcga accattcgtc ccatgtctct
      661 ccggctggcg taatgattct ggttcgtccg acagcaatca cttcaacgcg ttttacgttt
      721 tccggcaatg caaccgcttt tggcagtctg accgcctggc tgcggttgct gagaaatacg
      781 gtggtttcca ttatttgcct ccttatgtgg atatgtcttg tgtatatcta ttgtgggatt
      841 gataccggga tatgtcaaag ggatatacgt ttatgatgag tatcgcgcag gtcagatcgg
      901 ccggaagtgc cggtaattat tataccgaca aggataatta ctatgtgctg ggcagcatgg
      961 gagaacgctg ggccggccag ggggctgaac aactggggct gcagggcagt gtcgataagg
     1021 atgtctttac ccgccttctg gagggcaggc tgccggacgg ggcggattta agccgcatgc
     1081 aggatggcag taacaggcat cgtcccggct acgacctgac cttctccgct cccaaaagta
     1141 tctccatgat ggccatgctc gggggggaca aacgtcttat tgaagcacat aaccaggccg
     1201 tggattttgc tgtccgtcag gtggaggcca gtgcctccac ctgacggacg gacagtcaga
     1261 aacggtgctg accggtaatc tggtgatggc actgtttaac cacgacacca gtcgcgatca
     1321 ggaaccacag ttacacacgc atgcggtggt gactaatgtt acgcagtata acggcgaatg
     1381 gaagacactg agcagtgaca aagtggggaa aacggggttc attgagaatg tgtacgctaa
     1441 tcagattgcc tttggcaggc tctaccggga aaaactgaaa gagcaggttg aggcgctggg
     1501 ctatgaaact gaagtggtcg gtaagcacgg tatgtgggaa atgccgggtg taccggtgga
     1561 ggccttttcc ggacgctcac agactatccg ggaggccgtc ggggaagacg cctcgctgaa
     1621 atcccgggat gtggcggcgc tggatacgcg taaatccaaa cagcacgtcg atcctgaagt
     1681 cagaatggcc gagtggatgc agacgctgaa ggaaaccggg ttcgacatcc gggcatatcg
     1741 tgacgcagcg gaacaacggg catatacccg cacgcagaca cccggacccg cttcacagga
     1801 cgggccggat gtgcagcagg cggtgacaca ggccattgcc ggattaagtg aacgcaaagt
     1861 gcagtttatg tatacggacc tgctggccag gacggtcggc atactgccgc cggaaaatgg
     1921 tgtgattgaa cgggcacgcg ccggtatcga tgaggccatc agccgtgagc agcttatccc
     1981 cctcgatcgt gagaaggggc tgttcacgtt cgggattcat atgctcgatg agctgtcagt
     2041 ccgggcactc agtcgtgaca tcatgaaaca gaaccgggtg accgtacatc cggagaaaag
     2101 tgtcccccgg acggccggtt acagcgatgc cgtcagtgtt ctggcacagg accgtccgtc
     2161 gctggccatt gtgtccgggc agggcggtgc agccgggcag cgtgagcggg tggctgaact
     2221 ggtcatgatg gcccgggagc aggggcggga ggtgcagatt atcgctgctg accgtcgctc
     2281 gcagatgaac ctgaagcagg atgaacggct gtccggtgaa ctgataaccg gacgtcgtca
     2341 gttgctggaa ggaatggcct tcacgccggg cagtaccgtt atcgtcgacc agggcgaaaa
     2401 actctccctg aaagagacgt taaccctgct ggatggtgcc gcacgtcata acgtacaggt
     2461 cctgataacc gacagcgggc agcgaaccgg tacaggcagt gcactgatgg ccatgaagga
     2521 tgccggggtg aacacatatc gctggcaggg gggagaacag cgaccggcca ccatcatcag
     2581 tgaaccggac cgtaatgtcg gctggccgga gattttgcgg ccagcgtgaa agccggagaa
     2641 gagagcgtgg cacaggtcag cggagtacgg gaacaggcca tactgacaca ggccattcgc
     2701 agtgagctga aaacacaggg cgtgctcgga cacccggagg taaccatgac cgccctgtca
     2761 ccggtctggc tggacagccg gagccgttat ctgcgggata tgtaccgccc gggaatggtg
     2821 atggagcagt ggaacccgga gacacgcagt catgaccgct atgtgacaga acgtgtgacg
     2881 gcgcagagtc acagcctgac cctgcggaat gcgcagggcg aaacgcaggt ggtgcgtatt
     2941 tcttccctgg acagcagctg gtcgctgttc cggccggaaa aaatgccggt ggcagacggt
     3001 gagcgtctga gggtgacagg gaaaattccc ggactccgcg tctccggcgg tgaccgcctg
     3061 caggtggcat ccgtcagtga agatgcgatg acggttgttg tgccgggacg ggctgaaccg
     3121 gccaccctgc ctgtgagcga ttcaccgttc actgcactga agctggagaa cggctgggtg
     3181 gaaacgcccg ggcattccgt cagcgacagt gcgacggttt ttgcctccgt cacacagatg
     3241 gcaatggaca atgccaccct gaatggtctg gcccgcagtg gtcgtgatgt ccggctgtat
     3301 tcctcactgg atgaaacccg tactgcggaa aaacttgccc gccatccctc ctttacggtg
     3361 gtttctgagc agataaaggc gcgtgccggt gagacattgc tggaaaccgc tatcagtctg
     3421 cagaaagccg ggcttcacac gccggcacag caggccattc atctggccct tcctgtgctg
     3481 gaaagtaaaa acctggcctt cagcatggtg gacctgctga cagaggcgaa gtcgtttgct
     3541 gcagaaggaa ccggttttgc tgacctggga ggggaaatca atgcgcagat aaaacggggt
     3601 gatttactgt atgtggatgt ggcaaaaggc tatggcacag gcctgctggt ttcccgtgcg
     3661 tcgtatgagg cagaaaagag cattcttcgc catattctcg aaggtaagga ggcggtcacg
     3721 ccgctgatgg agagagtacc tggcgaactc atggagaaac tgacatcagg acagcgcgcc
     3781 gccacccgta tgatactgga aacgtctgac cgtttcacgg tggtgcaggg ctatgccggt
     3841 gtgggtaaga ccacacagtt ccgggcggtg atgtcagccg tgaacatgct gccggagagt
     3901 gaacgtcccc gtgtcgtggg gctggggccc acacaccgtg cggtcgggga gatgcgcagc
     3961 gccggcgtgg atgcacagac actggcgtcc tttctgcatg acacgcagct gcagcagcgc
     4021 agcggagaaa ctccggattt cagcaacacg ctgttccttc tcgatgagag ctctatggtg
     4081 ggcaataccg acatggcacg ggcatacgcc ctgattgcgg ccggtggcgg tcgggctgtg
     4141 gccagcggtg acacggacca gctgcaggcc atcgcgcccg gtcagccttt ccgtctccag
     4201 cagacgcgca gtgctgccga tgtggtcatc atgaaggaga ttgtgcgtca gacgccggaa
     4261 ctgcgggagg cggtatacag cctgattaac cgggatgtgg aaagggcgct gtccgggctt
     4321 gagcgtgtga aaccgtctca ggtgccacgt ctggagggcg catgggcacc ggagcactcc
     4381 gtgacggagt tcagtcacag ccaggaagcg aaactggcag aagcgcagca gaaggcgatg
     4441 ctgaaaggcg aggcttttcc ggatgtcccc atgacactgt atgaagccat tgtccgcgac
     4501 tataccggca ggacaccgga agcacgggag cagacgctga ttgtcacgca cctgaatgag
     4561 gaccggcgcg tactgaacag catgattcat gatgcacggg aaaaggccgg agaactgggg
     4621 aaggtgcagg tcatggtgcc tgtcctgaac acagcgaata tacgcgacgg ggagctgcgt
     4681 cgtctctcca cctgggagaa taacccggat gcccttgccc tggtggataa tgtgtatcac
     4741 cggattgccg gtatcagtaa ggatgacggg ctgataaccc tgcaggatgc ggaaggtaac
     4801 acgcggctga tttcgccccg ggaggcggtg gctgaaggcg tcacgctgta caccccggac
     4861 accatccggg tggggaccgg tgacaggata cgcttcacga agagtgaccg ggagcgcggt
     4921 tatgtggcca acagcgtctg gacggtgaca gcggtttccg gtgacagtgt cacactttct
     4981 gacggtcagc agacccgtgt gattcgcccc ggtcaggagc gggcagagca acatattgac
     5041 ctggcctatg ccatcaccgc ccacggtgca cagggggcga gtgaaacctt tgccatcgcg
     5101 cttgaaggca cggaaggtaa ccggaaactg atggccggct ttgagtcagc ctacgtggcc
     5161 ctgtcgcgta tgaagcagca tgtgcaggtg tacaccgata accgtcaggg ctggacggat
     5221 gccatcaaca atgccgtaca gaaaggaact gcccacgatg tgtttgagcc gaaaccggac
     5281 cgggaggtca tgaatgcaga gcggctgttc agtacggcgc gggagctgcg ggacgtggcg
     5341 gcaggccgtg ccgttctccg tcaggcgggg ctggccgggg gagacagtcc tgcacggttt
     5401 attgctccgg gacgtaaata tccgcaaccg tatgtggcac tgccggcgtt tgaccgtaac
     5461 ggcaagtccg ccggtatctg gctgaacccg ctgaccacgg atgacggaaa cgggctgcgg
     5521 ggattcagcg gtgaaggtcg ggtaaaaggc agcggggatg cgcagttcgt ggccctgcag
     5581 ggcagccgta acggagaaag cctgctggct gataatatgc aggatggcgt ccggattgcc
     5641 cgtgataatc ctgacagtgg tgtggtggta agaatcgccg gtgaaggccg tccgtggaat
     5701 cccggtgcca tcaccggtgg ccgcgtgtgg ggggatatcc cggacaacag cgtccagccg
     5761 ggagccggaa atggtgaacc ggtcacggca gaggtactgg cacagcggca ggctgaagag
     5821 gccatccgcc gtgaaacgga acgtcgcgca gatgaaattg tccgtaaaat ggcagagaac
     5881 aaacctgacc tgccggacgg caaaacagag caggctgtca gggaaattgc cgggcaggag
     5941 cgtgaccggg ctgccataac tgaacgggaa gccgcgctgc cggagagtgt actgcgtgaa
     6001 ccacaacggg tgcgggaagc ggtccgggaa gttgcccggg aaaatttgct gcaggagcgt
     6061 ctgcagcaga tggagcggga tatggttcgt gacctgcaga aagagaaaac cccgggcgga
     6121 gactgataca ggaagacaaa cactgatgac aacggataac acgaacacga cacgtaacga
     6181 ttcactggct gcacggaccg atacctggtt gcagtcattt ctggtctggt cacccggaca
     6241 gcgggatatc atcaaaacgg tggcactggt gctgatggta ctggaccaca tcaatctgat
     6301 attccagctg aagcaggaat ggatgtttct ggccgggcga ggagcctttc cgctttttgc
     6361 cctggtgtgg gggctgaatc tgtcccgtca tgcgcatatc cggcaaccgg ccattaaccg
     6421 gctgtgggga tggggaatta ttgcccagtt cgcatattat ctggcaggct ttccttggta
     6481 tgaggggaat atcctgtttg cctttgcagt ggcggcacag gtgctgacgt ggtgtgaaac
     6541 gcgcagtggg tggcgtacag cagcggccat actcctgatg gccctgtggg ggcctttgtc
     6601 cggcaccagt tacggtattg ccgggttgct gatgctggca gtcagcaacc ggctgtaccg
     6661 ggcggaagac agagcggaac gtctggcgct ggtcgcctgc ctgctggctg ttattccggc
     6721 gcttaacctt gccaccagtg atgcagcggc ggtggccggt cttgtgatga cggtgctgac
     6781 cgttggtctg gtgttgtgtg ccggaaaatc attaccccgt ttctggcccg gagacttttt
     6841 cccgacgttc tatgcctgtc atctggctgt gctgggcgtt ctggcgctgt gacgggtgtg
     6901 gtatctttgg ccgcaagagg atgattcgtc agaggcagaa cacagcatga cagagcagaa
     6961 gcgaccggta ctgacactga agcggaagac agagggaaca gcgcctgtcc gcagccggaa
     7021 aaccatcatc aatgtcacca cgccaccaaa gtggaaggtg aaaaagcaga agctggccga
     7081 gaaagcggcc cgtgaagcag agctggcggc aaaaaaagcg caggccagac aggcgctgtc
     7141 catttatctg aacctgccca cgctggatga ggctgtgaat accctgaagc cctggtggcc
     7201 gggattattt gacggtgaca cgccccggct tctggcctgc ggtatccggg acgtgttact
     7261 ggaagacgtg gcgcagcgga atatcccgct ctcgcataaa aaactgcgca gggcgctgaa
     7321 ggccatcacc cgttcagaaa gctatctgtg tgccatgaaa gccggtgcct gccggtatga
     7381 cacggaaggg tatgtgacgg agcatatttc tcaggaggag gaagcgtatg cgggagcgcg
     7441 tctggcgaaa attcgtcgcc agaaccggat aaaggcagaa cttcaggccg tgcttgatga
     7501 gaaataaaaa aagcctcccc cagagagagg aggcagggaa ataaggctta aaaggaaata
     7561 acttccgctt ccgaaaggaa gccagtccac tgaattattt atcacaataa tactgacaac
     7621 taaaggggga caagaaaatg aatggattca gaaacagttc acggaacggc caggtctggc
     7681 gttaccagcg tgccggcggg cgggccgtta ttctggaagt cagtggacgc tggatggaag
     7741 cggcagaagc atggcgacgg gctgcctgtg ttgcgcctag gacagactgg cagcagtttg
     7801 cccggaaaag ggctgaacac tgccaccggc gctgccgggg aagggtgtaa cgaaaaaagc
     7861 agccttcatt actctaggag tcagaaggct gaaaggagtt tatgagattt gcttcttaat
     7921 atccggtcgg gcagtatgac cggacagttc tgagtagcac agagtctttt ccgcattaag
     7981 tcgatttttt atattttgtg attagtttca caaaataagg tgttattcaa tgtgtgctgc
     8041 actattcagg ggggctgaaa tacaggtggc atttatctga tatggagaat aacatgagga
     8101 aatatattcc actggcatta tttatctttt catggccggt attgtctgca gatattcatg
     8161 gacgggttgt tcgtgtactg gacggtgata ccatcgaggt catggactca ctgaaggcgg
     8221 tgcggatcag gctggtaaat attgatgcac cggaaaagaa acaggattat ggccggtggt
     8281 caacagacat gatgaaatca ctggtcgcag gaaaaacggt taccgtcaca
//
LOCUS       DAJTSA010000149         7873 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000149 DAJTSA010000000
VERSION     DAJTSA010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7873
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.149"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(12..705)
                     /gene="ospZ"
                     /locus_tag="OO369_003758"
                     /pseudo
     CDS             complement(12..705)
                     /gene="ospZ"
                     /locus_tag="OO369_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            761..913
                     /locus_tag="OO369_003759"
                     /pseudo
     CDS             761..913
                     /locus_tag="OO369_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2294..3241
                     /gene="icsP"
                     /locus_tag="OO369_003760"
     CDS             2294..3241
                     /gene="icsP"
                     /locus_tag="OO369_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR5983871.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3803..4175)
                     /locus_tag="OO369_003761"
                     /pseudo
     CDS             complement(3803..4175)
                     /locus_tag="OO369_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4419..4538
                     /locus_tag="OO369_003762"
                     /pseudo
     CDS             4419..4538
                     /locus_tag="OO369_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4788..5654
                     /gene="ospB"
                     /locus_tag="OO369_003763"
     CDS             4788..5654
                     /gene="ospB"
                     /locus_tag="OO369_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR5983872.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5983..6723
                     /gene="phoN2"
                     /locus_tag="OO369_003764"
     CDS             5983..6723
                     /gene="phoN2"
                     /locus_tag="OO369_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR5983873.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(7083..>7873)
                     /gene="ospC4"
                     /locus_tag="OO369_003765"
     CDS             complement(7083..>7873)
                     /gene="ospC4"
                     /locus_tag="OO369_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_156647404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR5983874.1"
                     /translation="DSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYV
                     HGNQGKTDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPVSLDRIINFV
                     FQLEYHIPRMLSTDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGN
                     KKADIALYLLSKFNFTKQDVAEMEKMKNNRYCNLYDVEYLLSKDGANYKVLEYFINNG
                     LVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFFIKKWSGIRQTI"
BASE COUNT         2469 a         1478 c         1473 g         2453 t
ORIGIN      
        1 attaatatac attaagtaac aggcattcga gccttgattt gagaaagcct gtcattcatt
       61 tgttttatgt agctatctat ttcctctgat gcataactaa aaagttctcc catttcacga
      121 gcaataataa tctcagattt aatagacttt aatctctggc gactcatctc ttaacacttg
      181 cataatctct ctttttatat catatagttc tataaaccga tttgcccgta gttctccttg
      241 ggaagaatca tttctgaata caacaattat atacaaaaaa tcactatttt ctttagagaa
      301 gatcttacaa ctaatctctg ggagctcaca taatgcatgt aagtcaggat ggtacaaact
      361 atccacagaa aattgcccca taggacgttt tccagatata actgtcaacc agcttccttc
      421 cggtgtactg gcttcggctc tggaaatagg ctgaaaatct tccggacatg gagaatacat
      481 tgtcttcata taattaagtc gtctggattt ctcattaata aactcctcta tttttaattg
      541 tgccatactt ctggttatac cagagatata cttcttgtca tatatgtttg gtgattcatt
      601 aaagtaacca tgttcaactc taggataaat atttctcact atatattcag tatttgcagt
      661 atttattgga aatacgttct taatattctt gatgggacta atcattttaa tctctatact
      721 caaatataaa cattaccatg aacaatataa ttgttcctaa gtggaatgtc tccacggaga
      781 acactttatc tatcgggaaa tagtgcgggc aacagtgttt aattatatcg agtgcaatta
      841 caatcgctgg cgacggcacc gttggtgtgg cggcctcagt ccggaacaat ttgaaaacca
      901 gaacctcgct taggcctgtg tccatattac gtgggtaggc tcacagagcc tcacttacga
      961 ctgcgtccat attatgtggg taggatcaat aaagacgttt tatatgtgtc tgcttgcagc
     1021 cagaaaattt ataccctgca ggtatcataa acatgcttga taacttaatt ggggctccct
     1081 cttcctttta tggtgtaatt gtttgattga atacttccgg ggatttcagt atgaaatgaa
     1141 gtatatttaa tatactttac aaaaagtttg tattagtgga ataaggacaa tagcttatcc
     1201 aaactgccaa gcctctttat tataagtaag atctggcact ggaatattct caaaaggact
     1261 aacatcatca ccacgacctt caaccaacca ttttattgca tttaaatccc agaatagttc
     1321 agtagtggtc aatttgggaa acgcacaata ttgtacggtt acgttatcgc aatagagtga
     1381 actctcgccc tatacggcgt tttcaaggat tagtccttaa tcggacaacc acattaaagc
     1441 ataataaata attaagcttt aataatatac tatagataca gttctattat aaaaaatact
     1501 attttaggtg atgcattaaa ttagtagaat atttattctt ttataaaagc accaatgaat
     1561 tgtccaagta aacatgagat aaaatgtgta gaggataact gagtttggtc actttaactg
     1621 tattagtcgc agtctgactc tcgactttaa aaggatggga attacctgta ctgatagtga
     1681 gaaagctttc cattgaaata agtcaaataa aagttgatgg cttcagtgct aaagtgaggt
     1741 gcccgctagc cgtattcact cgaatcggaa ttcaatgtat tgcttctgcc atttccataa
     1801 tatttcacgg gaaaaacaaa atctgaaaaa ctatacttaa gtgttgtatt gagtcagttg
     1861 agatacaaat acatatttat cagcacacgt tacgtcaaat acaggaggtt gtcatgttgg
     1921 tactgaaagg cacgttggct attgcagccg tgatgacaat ttattgtctt atcgttgttc
     1981 ttatggaacg cttttctgat tgatttcata ttggcgagag aacaagaagg tgagtagaat
     2041 tgtcgcggaa tcctgaaact atcagcctca ggtatgaaca ccaaaaggta ggtagagaaa
     2101 tctatggccc ccgttacttt attcgcacaa tgaggtattt ttttattttt acaaaagttc
     2161 aataaatatt ataataaaag atatgttctt ggtaacataa acaaatattt aattaaaata
     2221 atgcttggca ttgacgttta tcttataaag taagaagatc atggacattt caaccaaaaa
     2281 agtagagttc tcgatgaaat taaaattctt tgtacttgca ctttgtgtac ctgcgatctt
     2341 tactacacat gctaccacta actatccact tttcataccg gacaacatca gtacggatat
     2401 tagtctagga tctctgagtg gcaaaacaaa agaacgcgtt tatcatccca aggaaggggg
     2461 gagaaaaatt agtcaactgg actggaaata cagtaatgcg actattgtta gaggtggcat
     2521 cgattggaag ctaattccaa aagtgtcttt cggagtttcc ggttggacta ctttaggtaa
     2581 ccagaaagca agcatggttg ataaagactg gaacaattcc aatacccctc aggtatggac
     2641 cgaccagagc tggcatccca atacgcatct ccgtgatgct aacgaattcg agctgaatct
     2701 taaaggttgg ttattaaata atttggatta tcgacttgga ctaatagcag gttaccaaga
     2761 gagtcgttac agttttaatg caatgggagg gagttatatt tatagtgaaa acggggggag
     2821 caggaataaa aaaggggcac atcctagtgg tgaaagaaca ataggttaca aacagctctt
     2881 caaaatacct tatattggat taactgctaa ttaccgccat gagaattttg agtttggagc
     2941 agaactgaaa tatagtggtt gggttctttc atctgataca gataaacact atcagactga
     3001 gacaattttt aaagatgaaa taaaaaacca aaattactgc tctgttgctg cgaatattgg
     3061 atactatgtc acccccagtg caaaatttta tatagaaggc tccagaaatt acatttctaa
     3121 taaaaaaggt gatacatctc tttatgagca aagtaccaat atatctggca ccattaaaaa
     3181 tagtgcaagt attgaatata ttggttttct cacttccgca ggtataaagt atattttttg
     3241 aattggttca ccgagttgat tacgttttca tggctaaaaa ttgccgccca ttgtgagtca
     3301 tattcgccct gactttccag aaccatacga actgcccgtt gacggacttc gggggaaaaa
     3361 cgtgtatttt tagtcattct gtttacctct ttctcagggc gtttagtctc caggatttcc
     3421 ggggcggttc aaaaaactct tcgttgatta ctgtggactt actgttggca ttaccgaccc
     3481 tgagaccgga gaaataagaa ccgcttagat catcgtggct gttctcgggg tatcaagtta
     3541 cacatgggca gaggctaccc aaggtctact attatcgcct gaaagcgctg atggaacagt
     3601 gctatcaggg acatgctgat gaaaatacct aaataatcag ccagcactct atctttccaa
     3661 atccacagca tagcaaagag agcaaaagag cctgtaaatt cagaaattaa ggaaacgcct
     3721 cttggtgtac ataatctatc ttatgttaaa tgaaatctaa tttcttgatt atatgctgga
     3781 tcgttcgtgg gtggctttgc ccctatattt ccagacacct gttatcattg gcccgctgcc
     3841 gcagatattc ccgtggcgag cgataatcca gcgcactatg cggatgccat tcgttataat
     3901 gctcgaatgc ctctgcaagg ttctttgctg ccgttaaccc gtcaggtttg ggcatgatac
     3961 tgatgtagtc acactttatt gttttcacga agctctttgc tattctgtta ctctccggac
     4021 tccgcaccgc cgtgttcttc ggttcaacaa agtcccaaca tccgggtgaa ctggcgtgtt
     4081 tcattagctc ggtagcatga accattatcc gtcagtcact tcactggaga cgccggaagc
     4141 tcgttgccga agcggtgttc caccgctccc agcatgtcgc caacgggaga gcatctacgg
     4201 tctgattgaa ctgtttattc aaattgtcca ccgtctgtca gtacgctctg aacgtcggct
     4261 ggttaaaaca ctgctcgctg attttcagag ggttcacggt aaaacagcac ttttattccg
     4321 gattgcggaa gcggctctga ataatccgga tgggctggtt aaagaggttg tgtaccatct
     4381 gcatattgtt gaaccagatc gcagcggtaa gagaagttca tcatacttgg cgcagttacg
     4441 cgatgtttct gctagaggtg atgccgtaaa gaacggcaga acattaccgg aacaagatag
     4501 cggtctacct gcactggtat cagacccggg gcttccccgg atgatctcca cggtacttta
     4561 ggtactgtgt ctatattatg taggtaggat caattaactt tgtagttgaa attctttcat
     4621 tgaaataatg aataaaaaag acaaaccgca ttaaacaatg ccagaatata attgcatttt
     4681 tgttccgttt ttttaaatca tagtatacaa agaagcttgc atactatatg aaaatataac
     4741 accctttact atgatgttta taaaacaata tatggagtca tgtaggtatg aatttagatg
     4801 gtgttagacc atactgtaga atagtcaata aaaagaatga aagcatatca gatattgcat
     4861 ttgcacatat aataaaaagg gtaaaaaatt catcatgtac tcacccaaaa gcagcattgg
     4921 tttttttagg agagaaaggt ttttgtgata gcaatgatgt tctatctatt atgggacaac
     4981 aaataccaag agtatttaag aacaagatgt tatatgatta tgtttttaaa aatgaaaaaa
     5041 gtaaaaatga ttttctaaaa atggctgaat catggctacc acagagtgaa ccaatagtaa
     5101 taaataatga tgatgacgca ttgaatgctg ctgcttattt ttctgtaaaa aaagcgaaaa
     5161 taaaaacagt aaacgatact gattttaaag agtataataa ggtttatatt cttgggcacg
     5221 gtagtcctgg ttctcatcaa ttaggccttg gttcggaact tattgatgta caaacaatca
     5281 tttcaagaat gaaagactgt ggtattctaa atgtgaaaga tatccgtttt acttcatgcg
     5341 gctccgctga taaagtggct cctaaaaatt ttaacaatgc ccctgctgaa agtctttctt
     5401 gtatccttaa ctctctgcct ttttttaagg aaaaagaatc tttgctagag cagataaaaa
     5461 aacaccttga aaacgatgag tcattgagtg atggtctaaa aatatccggc tatcatggat
     5521 atggagttca ctatggtcaa gagctttttc cctactcaca ttatcgttca acttcaattc
     5581 ctgctgatcc ggagcataca gtaaaaagaa gctctcagaa aaagactttt attattaata
     5641 aagaactgga ttagtataaa atttttaacc tatagttaat tgtttgcatt taagatgaag
     5701 ttattttatc tttagtagaa aagtgcttcc ctatgaggga aaatcacaaa tcatcataat
     5761 acagagacaa aacgatacga aaaaatagat aaaaaacatc attcttttac cacattattt
     5821 tccgtgaata tgaaaaataa tgttattact ttaatataag actatttttt gtttttccat
     5881 cactctgttc aaatttttcc gcatgacttg tgttttttgt aatacagctc gttttttaca
     5941 gctgaccaaa atcatcaatt aattatgcta aggaaataaa ttatgaaaac caaaaacttt
     6001 cttctttttt gtattgctac aaatatgatt tttatcccct cagcaaatgc tctgaaggca
     6061 gaaggttttc tcactcaaca aacttcacca gacagtttgt caatacttcc gccgcctccg
     6121 gcagagaatt cagtagtatt tcaggctgac aaagctcatt atgaattcgg ccgctcgctc
     6181 cgggatgcta atcgtgtacg tctcgctagc gaagatgcat actacgagaa ttttggtctt
     6241 gcattttcag atgcttatgg catggatatt tcaagggaaa ataccccaat cttatatcag
     6301 ttgttaacac aagtactaca ggatagccat gattacgccg tgcgtaacgc caaagaatat
     6361 tataaaagag ttcgtccatt cgttatttat aaagacgcaa cctgtacacc tgataaagat
     6421 gagaaaatgg ctatcactgg ctcttatccc tctggtcatg catcctttgg ttgggcagta
     6481 gcactgatac ttgcggagat taatcctcaa cgtaaagcgg aaatacttcg acgtggatat
     6541 gagtttggag aaagtcgggt catctgcggt gcgcattggc aaagcgatgt agaggctggg
     6601 cgtttaatgg gagcatcggt tgttgcagta cttcataata cacctgaatt taccaaaagc
     6661 cttagcgaag ccaaaaaaga gtttgaagaa ttaaatactc ctaccaatga actgacccca
     6721 taaagctgga cagcctgtat caggctatgg agggcccata gacaaatcta ccctatatga
     6781 gcataggagg agtctatggg cacaccacgt tttacccctg aatttaaggg attactggaa
     6841 aggctgggac atatccctcc ggcagaagca gaaaaagctt attatgctgc catcggaaac
     6901 gatgatctgg caacctgagt tcacagataa aacattctct aggaaactcg gggcggttcc
     6961 gttcaccaca tgcaatgtgg tgttgcaggg gaacggtctg caccatcccc tatgtcgatc
     7021 aatataacag aaatgacaac ttcagattca gggcacaacc taaatatatt ttaggtcacc
     7081 tctcaaatcg tttgcctgat accgctccat tttttaataa aaaaatcaat catttttgaa
     7141 tctttgcttt tcattgcatt gtcaagcata gtgtccccac tatttgcttt ttgaaatttt
     7201 ttgtttacat caaccaatcc attattgata aaatattcta gcactttata gttggcacca
     7261 tctttactaa gtaagtattc aacatcatac agattacaat atctattgtt tttcattttc
     7321 tccatctctg cgacatcttg ttttgtaaaa ttaaatttag atagtaaata aagtgctata
     7381 tcagcttttt tattgcctaa agaataagca agggcctgat gagccatttt tttatcagtc
     7441 actttattgt taatctcttc ataattagat gctttaatcg catcctccaa agatatgtct
     7501 ctgagtttta tttttttgaa gttatctgta cttagcattc ttggtatatg atactcaagc
     7561 tgaaacacaa agtttataat tctatccaga ctaacaggat caatattttt gttataacaa
     7621 aactctctga atccttgatc tttactcttt cttataaaat caataagatg caacccaatt
     7681 ccaagcctca tatctttagt gttaaatatt ggaacatcag ttttcccctg attaccatgc
     7741 acatacctgc taacttcatc cacaacctct ttaaagttat ctaagaaaaa tgattggtgc
     7801 tcatggtaaa aaacaggtgg aatagcatta tcaaagtgat cggttaatgt catatatcca
     7861 tatggagagt cat
//
LOCUS       DAJTSA010000150         7781 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000150 DAJTSA010000000
VERSION     DAJTSA010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7781
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.150"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1331
                     /gene="yjeM"
                     /locus_tag="OO369_003766"
                     /pseudo
     CDS             1..1331
                     /gene="yjeM"
                     /locus_tag="OO369_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="OO369_003767"
     CDS             complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="OO369_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR5983875.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WLVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4815..5783)
                     /gene="psd"
                     /locus_tag="OO369_003768"
     CDS             complement(4815..5783)
                     /gene="psd"
                     /locus_tag="OO369_003768"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR5983876.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="OO369_003769"
     CDS             complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="OO369_003769"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR5983877.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7027..7572
                     /gene="orn"
                     /locus_tag="OO369_003770"
     CDS             7027..7572
                     /gene="orn"
                     /locus_tag="OO369_003770"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR5983878.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         1763 a         2016 c         2104 g         1898 t
ORIGIN      
        1 ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat ctattcctgg
       61 atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg gttttcctct
      121 tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc aacattcctc
      181 tatggtagtg acatgaccca gcactggcgt attgccggac tggagcctac gcaggtggtt
      241 ggtctgctgg cagtagcatg gatgattctg gtcacagtcg ttgcttcaaa ggggattaat
      301 aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa tttagtattg
      361 ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca ggatattaat
      421 ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc gtttgtggta
      481 tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga taaaacggaa
      541 aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat ttcaatcggt
      601 tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt attaagtaat
      661 ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg ggtgatgctg
      721 ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg gtttgcgcgt
      781 attaccggac tttcgatgtt cctcgcttat accggtgcgt ttgctattca ccgctgaaag
      841 ccatcatcca ggggacgccg aaagcgttgt ggccggaacc gatgacgcgc ctgaatgcga
      901 tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt ggttactgtc ttcattctgc
      961 tggtttcgtt tggtggcggt accgcatcgg cgttctttaa caagctgacg ctgatggcga
     1021 acgtgtctat gacgcttcct tacctgttcc tcgcgctggc tttcccgttt tttaaagcac
     1081 gtcaggatct cgacagacca tttgtgattt tcaaaacgcg tatgtcggca atgattgcga
     1141 cggtggttgt cgttctggtg gtgacatttg cgaacgtctt caccattatt cagcctgtgg
     1201 ttgaagccgg agactgggac agcacattgt ggatgattgg cggccctgtc ttcttctcgc
     1261 tgttagcgat ggcgatttac cagaactatt gcagtcgcat ggcgaataaa cctgagttag
     1321 ctctcgactg atcatatgaa atcctgcaac tcatcgtctc tccggcgatg agttgtcagg
     1381 aagtaatgcg tatggatgat actctccagg gccggtgata actgaagatg atgttaagct
     1441 gctaaatgag ccacgttgac atcatttggc agcttcatgc attacaaact tcccgcctga
     1501 cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt taccgttgag gctttccaga
     1561 cgcatctgga agggcgggaa tggcatatcg ataccgtggg cgtggaagcc tgccagaatc
     1621 agctggtgga tctcatggcg tagcggcata cggtgaccca tctcagcggc gtaaatacgc
     1681 agctcgaaaa tctgaatccc ctgttgcaga tccaccagga agacttccgg tgccgggttg
     1741 tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga tttccgtcac ttcttcactg
     1801 ttggcatcgg caggggccgg aatcgtcaac actacgcgcg tgaccgagtc agagagcgac
     1861 cagttgataa actgctcggt aataaacgcc ttgttcggca cgattatctc tttacggtcc
     1921 cagtcgctga tggtggtggc gcgggtgtta atttttgtca cgctaccagt aagatcgcgg
     1981 atcgttaccg tatcgccaat gcgaatcggt ttttcgaaca ggatgatcag gccagagata
     2041 aagttggcga aaatttcctg caaaccaaaa ccgagaccaa caccgagcgc ggcaaccagc
     2101 cactgcaatt tcgaccactc aataccaatc attgagaagc cgaccagccc gccaatcagc
     2161 atcagcagat attttgtgat ggtggtgatg gcgtaacccg tacccggcgt taaatccagg
     2221 tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc gcaccagctg cgtggtgatg
     2281 ataaatacca gaatggcaat cagcaccgca ccgagggtaa ttggctccag actttctacg
     2341 ccctgtaccg tggaggtgac atcccacagc gaaatatttt cgaggaagcc gaaagcggaa
     2401 tggatttctg accacagcac aatgaccgaa agcagggcga tcaacatcaa aattgagcgc
     2461 accagccgca aggattgcgc actgatggca tcgagatcga cttcgctttc atcgacttca
     2521 attgctcctt ccgggctact gtgatgatgc gcttcctctt cgccacgcgc acgttgcgct
     2581 aacatctctg cccggcgatg cttcgcccgg tcaaacgcca gcctgcgacg ctggatcagc
     2641 atccagcggc ggataacgtg ataaaccacc agtagcagga accagatggc aaccgaggtt
     2701 tcaagcctcg ctaacagtgc ctgcgccgtt gccagataac ccaccgccga cgccagaatg
     2761 gcaaccagtg gtgcgccaat catcatgttc cacagcatat ggttggtaat gttgtcgccg
     2821 ctgccttctt tgttgagata caacggaata ccggcttttt tcaggcttag ggtgaccacc
     2881 gccagcgcac cgcaaatgag gataaagcaa agccgtccca gcgaaccgga aaattcgcgg
     2941 tcgtcgaggt tatcgaacat catcagcgcc ataatcagcc cgatgctcat caggtagtag
     3001 cgcatcccac gggaaacacg ttcgcgcggc cagccaaaat gagcgataaa caagccgttc
     3061 gggcgggcaa aggtggcgca aatcatcact aaccacagca gcggcacggt ggccgttaca
     3121 ccatcgccaa tcgcgaccgc cagcggataa ggccacgcct cgcgcaagcc gtaacccagc
     3181 gtcatccaca gcaccggtaa cggtgacgcg acgagaatcg accagaaaag agtgcgcaac
     3241 gtcagccaga agtgatcctg agtcactttg ccgactttcg ccgccgaacg ttcaagaaaa
     3301 cgggtgaaat agcggcgcga gtaaatactg caaccgacca gaatcaacgc gccaaacagc
     3361 ggcaaaattg tctctttgct ggtcagcatc atcacactgg ctttgcccaa ctgactgaag
     3421 gtgtccagcg aaatgagacg acgcagatcc tgggcgattt ccagcggcca ggcgatggtc
     3481 atcgggcgca cgtcagaggc ccagaacaga tagcggtgcg ttgcttcgtt cacctctttc
     3541 agcgcatcct ccagttgcct gttggagact ttcagcttgg tcagttccaa tagtagcgtg
     3601 tcgccaccct gcaacaatga gttcagcaac tcacgctgag tgcgcagttg tgcttccaga
     3661 atacggtttt gctcggcggt cagcggctga ccgtcggcct gatggatttg ccgtagcagc
     3721 ggctgtttat taagtaaatc ctcataacgt aaccgttgca cacgcaactg cgccatttcg
     3781 gtatcaagct gttgtggttt tggcatttct ggtagccgtg ccacctgcgc ccgcagcgct
     3841 tcgccgagca ggttggacga tcccagccat tgcgactgtt cacgcagcgt attcaacgcc
     3901 tgccggacct gtaacgtctg gctggcagcc tgacgctgtt gcgaggcaac gagctccatc
     3961 cgctgcgcct gttgattcag cgctgccgat agttcgcggt taattttgaa ttgcgcgacg
     4021 atatctttcg gcaaatcggc actgttttct gccagtaatt cggtactttc cagcgcccgc
     4081 tccgcctcaa gctgacgttg gctattcaac tgattacgta aggcctgcaa atacgcatcc
     4141 agttgctggc tctctttttc cgccagctct gagcgtaagc gcgctaattc ctggcggtta
     4201 ttggcagaca gctgcgccag ctccagttca tcaacgagcg ccttaagacg tgcagagtca
     4261 gactgcaacg cgaaattttg tgcctgattg agcggagtat tgccggtaag cgttcccagg
     4321 cggcgctcga tctcatttaa ctgacggcgg gcgtcggttt gctgttgcgg cagttgattc
     4381 agcgaatcgg caatctcgcg ggcgcgctcc tgctcttgct gggcctgacg gcttttatcc
     4441 agcaactggc tgctgacctg gagaatttcc tgattcagcg cgtcggtaga cattcccggc
     4501 gacacgctgc gcggctcgtc acgaatgttg tttaattgtg cgcgcagagt agcggagagt
     4561 ttcggataat tatcgataac ttgctgatat tgtttgatgc gctcaaggga accttttcgt
     4621 tcctcaagcg catttaaggc agactggagc gcctctacga cttccggctg tgcgggtttc
     4681 gccgcttttg cctgctccag ttcctgagtg atttgtttgc tatcgggggc cgtcgcggcg
     4741 tacgcccccc aactgaggca ccaggccatc agaaaagtga taatcaggcg cacgtcagcg
     4801 tttcctttga tggattagac ctggtctttt ttatcgtcaa ccaatgggct ggcgtcgtgt
     4861 tctgcttcga tctcttcagc aggaagtggg gcgggttcag cgtctggcgt aacaaaggtt
     4921 tcggtagata ctgccagcgg ctggccaatt ttcgtgacag acaggctttc cagttgctca
     4981 accagattca ctttacccgg tgcaaacagg ttgataacgg tggaaccgag tttaaagcga
     5041 cccatttcct ggcctttcag cagtgccaca gaaccgtcgt tttccccggc aggccaggtc
     5101 cagcgcttga tgataccttc gcgcggcggc gtaatggtgc ccgcccagac cgtctcaatg
     5161 ctgccaacaa tcgtcgctcc gaccagaatc tgcgccattg ggccaaattc ggtatcgaaa
     5221 aggcaaatca cgcgttcgtt acgggcaaac agatttggca cgttctgagc ggtgagatgg
     5281 ttaacggaaa agagatcgcc cggcacgtag atcatctcac gcagaatacc gttgcacggc
     5341 atgtgtacgc gatggtagtc acgcggggag aggtaagtgg tcacaaacgt accgttgcgg
     5401 aacaggtccg ccatcagata gttgcctgcc agcagggctt cgaggctgta gttgtggcct
     5461 ttggcttgca ggattttatc ttcttcgatt ttacccaact ggctgataac gccatcggca
     5521 ggcatgacca gtacattcgg atcggtatcg attgggcgta cttcgtcacg cagcggacgg
     5581 acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg gcttttgcgc ctctttcatg
     5641 tcgaccttgt agtatttaac gaacagatcg ataaccagtt ttgtcagcca tcctgcccgc
     5701 ttgcttgcgc cccaacccgc caggcgagta agccatagtt tcggcagaat gtactgtagc
     5761 gaaagtttaa atgaatttaa caaggtagcc tccaggccat tgttttgtcg ttcctgatcc
     5821 ggccaacatg ccggatcctg aaaaaaaggg gacgattcta acgacggtta gcttaattgt
     5881 cagtcatccg tatcagaaaa gtttttacgc gtttttacct gcgccatgct ttccagaata
     5941 cggtgatagt tttcgaaacg ggtttccgcg attttccctt cctcaaccgc ttcccggata
     6001 gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt tacacagacc taaatagtca
     6061 tggaattcga caaagccctg agtgatttgt tccggctcca ggtgccagag gccgaactca
     6121 cgcacaccag gggaatcaat cacatcaccg ccgtgcggga agtgatacag ccgagcggcg
     6181 gtcgtggtgt gctgaccgag ccccgagttg tcagagacat cgttggtcag gatctctttt
     6241 tgcagcccca gcaacgcgtt cagcaggctg gatttaccga cgccagactg cccggcaaaa
     6301 atgctgatgc gcccggtcaa cgcctcttcc agcggtttca gcccatcctg agtatggctg
     6361 gaaaccatca atacgcgata accgatgttg cggtagatat ccatctgctc gttgacgaac
     6421 gccatgcctt cgtcgtccag caggtctatc ttgttgagca cgataatcgg ctcaatctgc
     6481 aaggtttcgc aggccaccag gtaacggtcg ataatattga gcgacagctc cggcaaaatg
     6541 gcggagacaa tgacaatctg gtcgatgttg gcggctatgg gtttcacgcc gtcgtagaaa
     6601 tccggacgcg ttaacaccga ggtgcgctca tgcaccgctt ccacgatccc tttgacattt
     6661 acgccttccg ccgccggttt acccggacgc cagactacgc ggtcgccggt taccagcgaa
     6721 cggatggtac ggcgaatatt gcagcggtga acgtcgccat cggcggattc cacatcagcg
     6781 tgcataccaa agcggctgat gacgatacct tcatcaggct cgccaaacag attgtcgtcg
     6841 tagtcgggct tctccttaga cgttttaaga cgacgctggt gattggcgtt cacgcggcgc
     6901 tgctggcctt tggagagttt atttttactc aatcgtactg actcctggtc gcccctgatg
     6961 ggcaaaacat ctatgataca cgcaattgtg gatcaatata gtcactgtga atgggtggaa
     7021 aatagcatga gtgccaatga aaacaacctg atttggatcg atcttgagat gaccggtctg
     7081 gatcccgagc gcgatcgcat tattgagatt gccacgctgg tgaccgatgc caatctgaat
     7141 attctggcag aagggccgac cattgcagta catcagtccg atgaacagct ggcgctgatg
     7201 gatgactgga acgtgcgcac ccataccgcc agcgggctgg tagagcgcgt gaaagcgagc
     7261 acgatgggcg atcgggaagc tgaactggca acgctcgaat tcttaaaaca gtgggtgcct
     7321 gcgggaaaat cgccgatttg cggtaacagc atcggacagg accgtcgttt cctgtttaaa
     7381 tacatgccgg agctggaagt ctacttccac taccgttatc tcgatgtcag caccctgaaa
     7441 gagctggcgc gccgctggaa gccggaaatt ctggatggtt ttaccaagca ggggacgcat
     7501 caggcgatgg atgatatccg tgaatcggtg gcggagctgg cttactaccg cgagcatttt
     7561 atcaagctgt aaaatttttc ctggtcacgt aagcgccgga atccacttgc ggcggcgtga
     7621 ttttgacgct aaatcgaacg tcttgctgat aatttcagca cttgagataa aaacgcaaaa
     7681 aaaacttttt tggggggttg cagagggaaa gatttctcgt ataatgcgcc tcccgtaacg
     7741 acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt a
//
LOCUS       DAJTSA010000151         7743 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000151 DAJTSA010000000
VERSION     DAJTSA010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.151"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1013
                     /gene="ydfJ"
                     /locus_tag="OO369_003771"
     CDS             <1..1013
                     /gene="ydfJ"
                     /locus_tag="OO369_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCR5983879.1"
                     /translation="LLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGT
                     NCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKV
                     NDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIP
                     TDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPST
                     IMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGI
                     FCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSR
                     SL"
     gene            1102..2562
                     /locus_tag="OO369_003772"
     CDS             1102..2562
                     /locus_tag="OO369_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5983880.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="OO369_003773"
     CDS             complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="OO369_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR5983881.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2979..3665)
                     /gene="ydfH"
                     /locus_tag="OO369_003774"
     CDS             complement(2979..3665)
                     /gene="ydfH"
                     /locus_tag="OO369_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR5983882.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="OO369_003775"
     CDS             complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="OO369_003775"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR5983883.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4637..6682
                     /gene="dcp"
                     /locus_tag="OO369_003776"
     CDS             4637..6682
                     /gene="dcp"
                     /locus_tag="OO369_003776"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR5983884.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6727..7245)
                     /locus_tag="OO369_003777"
     CDS             complement(6727..7245)
                     /locus_tag="OO369_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR5983885.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFACHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7521..>7743
                     /gene="ydeI"
                     /locus_tag="OO369_003778"
     CDS             7521..>7743
                     /gene="ydeI"
                     /locus_tag="OO369_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCR5983886.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHK"
BASE COUNT         1940 a         1812 c         1957 g         2034 t
ORIGIN      
        1 tcttgctggt gacgctgcgt attattcagg gattgggtgc aggtgcggaa atttccggtg
       61 ccggtacgat gctggcggaa tatgcgccaa aaggtaagcg cggaattatc tcctcatttg
      121 tggctatggg aactaactgc ggcaccttaa gcgcaacggc aatctgggcc tttatgttct
      181 tcattctcag taaagaggaa ctgctggcgt ggggatggcg tataccgttc ctggcgagcg
      241 ttgtcgtgat ggtctttgct atctggttgc gtatgaatct gaaagaaagc ccggtctttg
      301 agaaggttaa cgacagcaac caaccgacag caaaacctgc acctgctggt agcatgttcc
      361 agagcaaatc cttctggctg gcaacagggc tgcgttttgg tcaggcgggt aactccggtt
      421 taattcagac tttccttgca ggctatttag tacagacgtt attgtttaac aaagcaattc
      481 caacagatgc attgatgatc agttcgattc tcggctttat gaccattccg ttccttggtt
      541 ggttatccga taaaattggt cgccggatcc cgtatattat tatgaatacc tctgcgattg
      601 tgctggcgtg gccaatgctt tctatcatcg tagataaaag ctatgccccg agcaccatta
      661 tggttgcact gattgtgatt cataactgtg cggtgctggg attatttgct ctggaaaaca
      721 ttaccatggc agaaatgttc ggctgtaaaa accgcttcac ccggatggct atttcaaaag
      781 aaattggtgg tcttatcgct tccggttttg gtcctatcct ggcgggtatt ttctgcacca
      841 tgacggaatc ctggtatccg atcgccatta tgatcatggc atattcagtg attggtttaa
      901 tctctgcgct gaaaatgcca gaggtgaaag accgtgattt aagtgcgctg gaagacgctg
      961 cggaagatca accgcgtgtt gtaagagctg cgcaaccttc cagaagtctg taaaccctca
     1021 accccttctc ttaccggaga ggggattttt attcatataa caaaacatat agcttgccat
     1081 atttaaattt aaggaattgt tatgggaaat aatttgttat cagcaaaagc gacactccct
     1141 gtttatgatc gtaataacct ggccccaaga attgttcatt taggctttgg tgcatttcac
     1201 cgtgcgcatc agggcgtgta tgccgatatt cttgctacag aacatttcag tgactgggga
     1261 tattatgagg tcaacttaat cggcggcgaa cagcaaattg ccgatttaca acagcaagat
     1321 aatctttata ccgttgcgga aatgtcggcc gatgcgtgga cggctcgcgt cgttggcgtc
     1381 gttaaaaaag ccttgcacgt acagatggat ggcttagaaa ccgtgttggc tgcgatgtgt
     1441 gaaccgcaaa tcgcgattgt ctctctgaca atcaccgaaa aagggtattt ccactctccg
     1501 gcgaccggac agttaatgct cgagcacccg atggtcgctg ccgacgtgca aaatccccac
     1561 cagccgaaaa cagcaacagg ggtgattgtt gaggcgctgg ctcgccgtaa agcggcagga
     1621 cttcccgcat ttaccgtcat gtcatgtgac aacatgccag aaaacggtca tgttatgcgt
     1681 gacgttgtca cttcctacgc gcaagccgtt gatgtaaaac tggcacaatg gatcgaagat
     1741 aacgtgactt tcccatcaac aatggtggac cgtattgtgc ccgcagtgac agaggatacg
     1801 ctggcgaaaa tcgaacaact taccggtgtg cgcgatcctg ctggcgttgc ctgtgaacct
     1861 ttccgccagt gggttatcga agataacttt gtagccggac gtccggaatg ggaaaaagcg
     1921 ggagccgaac tggttagtga tgtgctgcct tatgaagaga tgaagttgcg catgctcaac
     1981 ggcagtcatt cattcctggc atatctgggt tatcttgctg gatatcagca cattaatgac
     2041 tgtatggaag atgaacatta tcgtcatgcg gcgtatggca tgatgttgca ggaacaagcg
     2101 ccgacgctga aagtgcaggg cgttgatttg caagattacg ctaaccgatt aattgcacgc
     2161 tatagcaacc cggcgttacg tcatcgaacc tggcagattg cgatggatgg cagccagaaa
     2221 ttgccacagc ggatgttgga ttctgttcgc tggcatctgg cgcatgacag caagttcgat
     2281 ctgctggcgc tgggcgtcgc gggttggatg cgttatgtcg gtggtgttga tgaacaggga
     2341 aatccgatag aaatcagtga cccactgtta cctgttattc agaaggctgt acaaagtagt
     2401 gccgaaggga aagcgcgcgt ccagtcattg ctggcgatta aggcaatctt tggtgatgat
     2461 ttgccagaca atagcttgtt tactgcaaaa gtgacggaag cgtacttgtc tttattagcg
     2521 catggcgcga aagcgaccgt ggcgaaatat tccgtgaagt aaaaaaagca atgccgccat
     2581 cggcggcatt cacacactta gttcatgccg agacgaatca ggtccagagg tgccagtttc
     2641 tcttcacaac cttcaacaac tacggttttt ccgcggcgga tttgctcagc ggtcagacct
     2701 tcagtatcaa tcgacaggat cttgccagtg tgaccggtgc cgctaaccat aacacggctg
     2761 ccagtggtga ttgcgttacg gttacgatcg taggtcgtca taggtgtttt ctcctttctg
     2821 atttacagtc gaagcccacg taacgggcgc aatataaata ctcctgtcga gtgaaatatt
     2881 ttttgttttt gatcaagctc acatcttttt tgatgcatgc atgagccgta aggcgggggg
     2941 aaaagcctct catcccggat gggatgagag taaatgaatt actcttcgct aaaccagtcg
     3001 ctgttttcct ggcggatctg gcgcacggat tcgctgattt cctgcaaatg ttgcgtcatt
     3061 gcacgttcta ccgcatcgcc atcacgtttt tgcagggcag agaaaatatc aagatgctgg
     3121 cgtaacagca tttctggtgg agaaacatgg tcgaaactca tatagcgcac gcgatcaacg
     3181 gtcgctttca ggttctcaat ggtatcccac gccagttgac agtcggcaat ctgcgtcagg
     3241 agttgatgga agttgtcatc aagttcaaaa aaatcatcca gttgcttgcg ctcaatggca
     3301 atgcgttgct ggtgaagatt ttgttccagt tggtagcact ggctttcggt aatcatgctc
     3361 gccgcccgac gcgccaccgc gcactcaatg gcctgacgga taaaactgcc gttgcgcacc
     3421 tgggccatgg aaattttgtt gacgtagctg ccacgctgcg gacgaatttg aatcaggccg
     3481 ttttccgcca gtttaataaa ggcttcacga accggctggc gtgacacatt gaaacgaaca
     3541 gaaacttctt tttccgacaa cggtgtgcct ggtgcaatca ggcaatggac aatgtcgcga
     3601 cgaagaatac gataaatctg ctgattgaca ggctgtgtgg gattaagttg cgtttcgacg
     3661 gtcattcgtt cttgcttgtg agtgagttaa ctgcatgagt ctaccacttt tttgtgacaa
     3721 gcaataaccc ggcagttacg ccgggtataa aaattactga cggtggacat tcagtccggc
     3781 atagctttgg gtaaccggca tcatttccag ggtattgata ttgacgtgag caggcagcgt
     3841 tgacacccac cagacggctt cgctgacatc ttctggcgtc aatgcaacgg tattttgata
     3901 ggttttttcc gctttaccgt catcgccttt aaagcggaca ttggaaaact cggtgccacc
     3961 caccagaccc ggttcgatgt cggtgacgcg caccgccgta ccatgcagat ccgtacgcag
     4021 attcaggcta aactgacgaa caaacgcttt cgtcgcaccg taaacgttac caccggcata
     4081 cggccagctg cctgccgttg agccaatgtt aataatatga ccatgattac gttcaaccat
     4141 acccggtaag acggcgcgcg tcatatatac taggcctttg ttgttggtat caatcatcgt
     4201 ttcccagtct tcaacgctgg ctttatgcgc aggctccatg cccaacgcca ggccggcatt
     4261 atttaccagg atatcaatat tgctccactc ggcagggagc gatgccagca tctcttcaat
     4321 agcggcgcgg ttgcgaacgt ccagttgggc gatatacaga ttatctccca gttcgtctgt
     4381 taactcctgc aaccgctcct ggcgacggcc agtggcgata actttatgcc cttgttgaat
     4441 aaaacgacga gtaatgcatt caccaaaacc tgccgttgct ccagttacta aaacgatcat
     4501 ctcactgttc ctcaacgctt ttgtgtgtca ataacatagc acgtgatgct taagagcggg
     4561 taatactggt ttgcaagatt gcactccgtt atcagtaagc ctacgctatg gaacacctcg
     4621 cgttcaggag aagaaaatga caacaatgaa tcctttcctt gtgcaaagca cactgccgta
     4681 tctggctcct cattttgatc aaattgccaa tcatcactat cgcccggcat tcgatgaggg
     4741 aatgcaacaa aagcgggcag aaattgctgc catcgcgctt aacccgcaaa cgcctgattt
     4801 caacaatact attctggcac tggaacaaag cggagaatta attacccgcg ttaccagcgt
     4861 cttttttgcg atgactgcgg cgcataccaa tgatgaatta cagcgtcttg atgaacagtt
     4921 ttccgctgaa ctggcggaac tggctaatga tatctatctg aacggtgaat tattcgcgcg
     4981 ggtagatgct gtctggcagc gccgtgaatc cctggggctt gatagtgaat ccatccgcct
     5041 ggtggaggtt attcatcaac gttttgtcct tgccggagcc aaacttgcac aagctgataa
     5101 agcaaaatta aaagtactga atacagaagc tgcgaccctg accagccagt ttaaccagcg
     5161 gttacttgca gcaaataaat ccggcggtct ggttgtgaac gatatcgcgc agctggcagg
     5221 aatgagtgag caagagattg cgctggcggc agaggcggct cgcgagaaag gtctggataa
     5281 caaatggctg attccgctgc tgaataccac ccaacaaccg gcgcttgccg aaatgcgcga
     5341 tcgtgcgacg cgtgaaaaac tgtttattgc gggctggacg cgagcggaaa aaaatgatgc
     5401 caatgatacc cgcgctatca ttcaacgtct ggtagagatt cgcgcacagc aggcgaaact
     5461 gcttggtttt cctcattatg ccgcatggaa aatcgccgat cagatggcaa aaacgccaga
     5521 agcagcactc aactttatgc gggaaattgt tccagcggcg cgtcaacgtg cgagcgatga
     5581 attagcctcc atacaggcgg ttatcgataa gcagcagggc gggtttagcg cgcagccgtg
     5641 ggactgggca ttttatgcgg aacaggtacg gcgggagaaa tttgatcttg atgaggcgca
     5701 gctcaagcca tattttgaat taaacacggt gttaaatgaa ggtgtattct ggaccgcgaa
     5761 tcagctcttc ggtattaagt ttgtcgaacg ttttgatatt ccggtctacc atcctgacgt
     5821 tcgtgtgtgg gaaatttttg atcataatgg cgtgggactg gcgttatttt acggtgattt
     5881 cttcgcccgt gattcaaaaa gcggcggtgc atggatgggc aattttgttg agcaatcaac
     5941 gcttaatgaa acgcatccgg taatttataa cgtctgcaat tatcagaaac ccgctgccgg
     6001 tgagcctgcg ttgttactct gggatgatgt aataacctta ttccatgaat ttggtcatac
     6061 gctgcacggc ctttttgccc gccagcgtta tgccacgctt tccggcacca acacgccgcg
     6121 tgattttgtc gaatttccgt cgcaaatcaa cgaacactgg gcaacgcatc cgcaggtatt
     6181 cgctcgctac gcccggcatt atcagagcgg ggcagcaatg cctgacgaac tgcaacagaa
     6241 aatgcgtaat gccagcctgt tcaacaaagg gtatgagatg agcgaactgc ttagcgccgc
     6301 acttctcgat atgcgctggc attgcctgga agaaaacgaa gcaatgcagg atgtcgatga
     6361 ttttgaattg cgggcgctgg tggcggaaaa tatggatctt cctgctatac cgccacgcta
     6421 tcgcagcagt tatttcgccc atatttttgg tggcggatat gctgcaggtt attacgctta
     6481 tctgtggacg caaatgttgg ccgatgatgg ttaccagtgg tttgttgagc agggcggatt
     6541 aacgcgtgaa aatgggcagc gttttcgcga ggcgatcctt tccagaggta acagcgaaga
     6601 tctggaacgc ctgcatcgac aatggcgtgg taaggctcct cagattatgc cgatgctgca
     6661 acatcgtggc ttgaacatat aaacaccacc aatggctggc ctggagatat cgccaggcca
     6721 gcctggttat agcagtaatt gcagcagctg ggcgagggca aacctcaccg ctaaagctaa
     6781 taccgtttcg cagtcgccag caaaatgcat aaccgcagtg tagttctggc ctttaatatg
     6841 gcacgcgaac cagacggtac ccgctggcgt accatcttca ccgccctccg gtccgccgta
     6901 gccggtaatg gcaatactga catcagcatc cgcacgctct atggcacctg ttgccatttc
     6961 tgctgccact ttctcactca ccgcagaata tcgttcaaga gactgctggc ttacgctgag
     7021 gattttcatc tttgcctgat cggtgaaagt aacaaagcct gcaccgtaaa atttgggtgt
     7081 atcttcagct gcacacaggg cgctagccaa cttaccgccg gtgcatgatt cggcggtagt
     7141 taagcgtaat tgatccgcga taagtctttg actcaacgcg gatgtcaggt tttcaatagt
     7201 gtcggtatct gcgagttgta caattttatc tttattaatg ttcactgttt tttgtccacg
     7261 ctattatatg taaaggtgat ttgctttttc tgtatgctcg tccaatataa taattttcaa
     7321 acatataaat ccgattattc tgaaaacatg tttaagactc ttcaatcttc ttaactttag
     7381 tgagtcaaat tctctgaata tttgacagct agtgggctga ttttaaatgc cttataacaa
     7441 ttggcagtat tcttatttgg caattataaa aatagcaacc agacttaata agtcaaacca
     7501 aacgcaatta aggagttagt atgaaatttc agtccattgt attagcaagt tttcttgtca
     7561 tgccttatgc gttggcagat gaccagggcg gtttaaaaca ggatgcagcg ccaccgccgc
     7621 ctcatgcaat agaagatggc tatcgcggaa ccgatgatgc aaaaaaaatg accgttgatt
     7681 tcgcaaaaaa catgcacgat ggcgcttcgg tttcactacg cggtaatttg atttctcaca
     7741 aag
//
LOCUS       DAJTSA010000152         7592 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000152 DAJTSA010000000
VERSION     DAJTSA010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.152"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..212)
                     /locus_tag="OO369_003779"
     CDS             complement(<1..212)
                     /locus_tag="OO369_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5983887.1"
                     /translation="MVTVNFHCPRCQSVQVYRHGQNPKGHDRFRCRDCHRVFQLTYCY
                     EARKPGVKNQITEMAFNGAGVRDTGRT"
     gene            complement(551..1105)
                     /locus_tag="OO369_003780"
                     /pseudo
     CDS             complement(551..1105)
                     /locus_tag="OO369_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1248..1370
                     /locus_tag="OO369_003781"
                     /pseudo
     CDS             1248..1370
                     /locus_tag="OO369_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1632..2198)
                     /gene="ipgB2"
                     /locus_tag="OO369_003782"
     CDS             complement(1632..2198)
                     /gene="ipgB2"
                     /locus_tag="OO369_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR5983888.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2480..2794)
                     /locus_tag="OO369_003783"
     CDS             complement(2480..2794)
                     /locus_tag="OO369_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983889.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(2872..2982)
                     /locus_tag="OO369_003784"
                     /pseudo
     CDS             complement(2872..2982)
                     /locus_tag="OO369_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3306..3983)
                     /gene="ospD1"
                     /locus_tag="OO369_003785"
     CDS             complement(3306..3983)
                     /gene="ospD1"
                     /locus_tag="OO369_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR5983890.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4355..4492)
                     /locus_tag="OO369_003786"
                     /pseudo
     CDS             complement(4355..4492)
                     /locus_tag="OO369_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4574..4872)
                     /locus_tag="OO369_003787"
                     /pseudo
     CDS             complement(4574..4872)
                     /locus_tag="OO369_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5461..5620)
                     /locus_tag="OO369_003788"
                     /pseudo
     CDS             complement(5461..5620)
                     /locus_tag="OO369_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5624..6853)
                     /locus_tag="OO369_003789"
                     /pseudo
     CDS             complement(5624..6853)
                     /locus_tag="OO369_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6873..7220)
                     /gene="tnpB"
                     /locus_tag="OO369_003790"
     CDS             complement(6873..7220)
                     /gene="tnpB"
                     /locus_tag="OO369_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5983891.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(7220..>7592)
                     /locus_tag="OO369_003791"
     CDS             complement(7220..>7592)
                     /locus_tag="OO369_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5983892.1"
                     /translation="RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQY
                     RKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRL
                     KGKLTPALLQILIREIKGSSH"
BASE COUNT         1961 a         1766 c         1644 g         2221 t
ORIGIN      
        1 gtacggccag tatcgcgaac tccggcaccg ttgaaagcca tttcagtgat ttggttttta
       61 actcccggtt tacgggcctc gtaacagtaa gtgagctgaa acacccggtg acagtcacgg
      121 caacggaatc gatcatgtcc ttttgggttc tgcccgtgac ggtaaacctg aacggactgg
      181 caacgggggc aatgaaagtt aactgtgacc atgaaagaac ctaaaagcgg gtattataac
      241 ccctaattca actaattgga ggcattgtca tttttaatct atggatacac ttaaaaatcc
      301 tctcacctta cgttaaaaac cttctcatgt ttagagtttt ttattatgtt tatcataatg
      361 atatgacgat ctgagttttt aaaatcaaga agaaaagata agttttttaa atgcacagtg
      421 cctgtggata actttgcgta aatccggagg acagatcact ctgaagcaag acaacagcat
      481 ctgaaaacaa tatgctcacg tttcaaccac agggcgtaag tgatcctacc cacgtaatat
      541 ggacacaggc ctaagcgagg ttctggtttt caaattgttc cggactgagg ccgccacacc
      601 aactgtgccg tcgccagcga ttgtaatcac attcgatata attaaacact gttgccctca
      661 ttatttcccg gctgataaag tgttctccgt ggagacattc cactttcagc gaatgaaaga
      721 agctttccac gcaggcatta tcgtagcagc agccctttgc gctcatactg ccacgcagat
      781 tatgccactt cagcagcgcc tgataatctg ctgaacagta ctgacttcct cggtccgagt
      841 gaacgataac gttccggggt ctcttacgcc gccacagcgc catctgcagg gcatcgcagg
      901 ccagttgcgc cgtcaggcgt ggtgacatgg accagccaat aacggcacgt gaccacaggt
      961 caatgaccac agccagatac agccagcctt catctgtacg taagtacgtg atgtctcccg
     1021 cccacttctg gttcggacca ctggcgtaaa aatcctgctc cagcagattt tctgacacag
     1081 gcaggccatg tgcgcggtaa ctgaccgtat tgctgggtaa gatcaatttg ctcactggta
     1141 tttactacgt gcatcgaatg agtgatcaag agccgtatga cgcgagagtg tcacgtacgg
     1201 ttccgtaagc agccggagag tgaaactctc tccggctgac tcgactcgca ggttccgaca
     1261 gcggtgacga acatgcagcg gtgttgttct cgctgatcgg cacatgccgt ctgaacaatg
     1321 tggagccaga aaaatggctg cgttacgtta ttgaacatat ccaggactga ccggcaaacc
     1381 gggtacgcga tctgttgcct tggaaagttg atctgacttc tcagtaaata tcaatatgat
     1441 tctgacgagc cgcttacctt tgagcagaga aaggggagag ggatcccaca tccatgagct
     1501 aggaaaatac aaaaactggc tatacaacaa ttcaatatat taggacttac ttgcgaggag
     1561 aaatatagca ccgctcaggc aattcctggg tatttacttt tcttagtttc tttactcggg
     1621 atgttctgtc atcagaaagg cgattctaaa tttgtaatat agtcactcat attcccacgt
     1681 aatttcttta gatcaatata ttgcacattt ttctcaaaaa actgatcgga aataagattt
     1741 gcaacattgc ttgttgtatc atttttaacg cttctattca tcccttcaca aagttcgtcc
     1801 attttctttt tgaaataact gcttcctgat acaagatgat ttatggaact ttgtgcagca
     1861 ttgatgtcta gagaaatgcg ataatctttc tcaacctgtt taaatatctg tttacggaga
     1921 ttatcgccga tttttcggct gtgttctgca caaaacttat caatacattg gtttaccacc
     1981 cgggatacgt agactttttt ttcatcattt ttccaatctg taatattttc accgatttgt
     2041 tctgaaacag agctcaaaat actagtattt attttggttg atgatatttt tactatcctt
     2101 ttccccattg tacagcggat aatctcatcc acctttcctg aaaaatatct tttgtgagat
     2161 aaactgattc caaaattatt aaaagatgtt ccaagcatta taatcacctt gtcatgtaaa
     2221 atagtaacat tgtcattatt ttagctgatt caaacaattc tgtaaatgct ttgagttctt
     2281 tactcccttg gctcaatgat ggaccgtctg gaatcccaga gactaaactc tctgagaaag
     2341 agagtatgaa aaaacttggg gcagtcccta aatgaaggga ccaacagcgg taaaataacc
     2401 ggcatcattc ataatgatgc cctgttagtt gtagttatga agatttacgt cattaaaatt
     2461 gctgttcgta gtgtcagtcc tatggtctgg catcagctga ggatcgtagc cgatacatca
     2521 ctggcttcac gacattcgtg ttgaggctat tcatgaaaac tccacgctga aaacgccgtt
     2581 ttgtataaac ggtcacggta tgcccggagc cactgttgct gataaattcg ataaaacctt
     2641 ggcgtttctt gaagctatcg ttaatgcgga taatgaaaca acgattggtg agattcgctc
     2701 ttttgccgac gccctggacg ctgtccggtt caatcgcaac aaaatcaacc ggcagctgag
     2761 caaacccaac cttgcctctc tggcgctgga gcatgaagtg atttggctgg gacgtagccg
     2821 ctgacagctt ctataaaagt atgatcctac ccacgtaata tgaacacagg cttaagtgag
     2881 gttctggttt tcaaattgtt caggactgag acctccacac caactgtgcc gtcgtcagcg
     2941 attgtaatcg cacttgatat aattaaacac tgtatatttc atttgtggag tctccagtct
     3001 gagagggctt tatgcattcc gtattgggca ctatgatgcc agaaatctgc aaggctccac
     3061 accttcctgt atttcccttc ctctatcaca gtgcacttta cttaataggg tgggatgtgt
     3121 tcattacatt tattgtgttt tttatgattg tttttgcgtc cagtgtcgtt cgtcatggga
     3181 aactggttct atgggtagta tcgctcagtc tagttaactg tttgtttctt tttagaaatt
     3241 aagcagatca cacaattcgg ggagagatcc ttctaagtgc aattctttgc agctatttag
     3301 cacacttaaa acaactcttg tattttgtca gaaataatat taaacatgtc ttgttcatta
     3361 ttgttaagtc tgtctgctat ttctgtaagt tttatcccac tatgatggca tatatttata
     3421 tatgccatca tagcctcgat attccctttc cctgcggcca gaaataatcc tggtaacccc
     3481 tcattatctc ttacatccaa tagtttaatc gtttcttttg gtgataaatc aaaagtttta
     3541 ataaaatctc cataagcttg aatcgcagcg gaatgacctt cttgaagagc tatgaacaaa
     3601 gcagattccc cagcattatt tcgtgctgca agaacttcta gtttttcctc tttgggtatt
     3661 tcggctgcca acagcgattc accgtattta cgcgctgcat cggcatttcc atgccaaatc
     3721 aatgaaaaca aattatggcg gttatactcc ttattattct ttgtcggaat ggatatgttc
     3781 tgtattttgg ccaattttct gaaagaaaca ccactatatc ccccccctga tttttcttca
     3841 ttgatggcgt gggatatagc ggtatttgtc acgttaatga tattattttt cattccttta
     3901 ttttcattag cagtattgct gcctattatt aagtgcatat ttttgttgtt tgctggatgt
     3961 aatccatagt tatttattga catttttgtt catccttttt gctaaaaaaa aacaataacc
     4021 tatttttgta atgtaataga tattagtcta ttgctaatct aaaaaaacca ttgccacatt
     4081 gaatggctat attcaccaat aaattctttc gatttcgtat attgacatat cgtagatata
     4141 tcttatccct aagattcaat aatcgagtcc gtttaccatt ctgtgcaagc gccttcactc
     4201 agaagtgacc gtcaaggcga tcaccaaact tgataataaa gcagctcatt gccatacgcc
     4261 agtccttaag gggcgttgtc catttctgtg atgctgccta gatttaactg ccagctccgg
     4321 cttatctaca tctaaatatg tgagcctaat accgctacac tttgtccaga ctatgttttt
     4381 ctccgggaag tgggctaacc gtctccataa agtgacgaac ttttttcaac agtggccata
     4441 ttgaacggca ctgatgatta cgggttattg tctcatgaag agccggccac atcactgaca
     4501 gcagtgactt caggccttca gcaccagaca gcctgaacca gttaatccat cgcccgacaa
     4561 atttagtaat ctagggctgg tttttcttca gctgttgcgg ttcgaaagtg ccattacgat
     4621 cacgcagagt acggatttcc agaggttcat ctccagtagt gataatcttc gttaagcaac
     4681 cattgcggga attgctgcca gatctaagct gatttttctc gcacccaaga tgatgagtca
     4741 tattagcatt gagtacggtt tcattttcag catccaatca aactgactga gatcttcagg
     4801 atttttgaga cttttggcta gttcattagc taaagcccgc aacttattag atgtcaactg
     4861 tttttcattc ataaattcac cttcatttga tatcaaatta aatagttcaa catcagagaa
     4921 ttatacaaac ttatgtacag gctcctatga tcttcgaatc ctgaagatta tagtgaaaat
     4981 aagttttcac gttgaaatcc cacatagtta gtatatgata aataatcatg gatgacctga
     5041 ttaacctctt ggggtagtgc ttttatagaa gaataaatat tctactaatt taatacatca
     5101 cctaaaatag tattgttttt tatggttggt tgaaggtcgt ggtgatgatg ttagtccttt
     5161 tgagaatatt ccagtgccag atcttactta taataaaggg gcttggcagt ttggataaac
     5221 tattgttctt attccactaa tacaaacttt ttgtaaagta tattaaatat acttcatttc
     5281 atactgaaat ccccggaagt attcaatcaa acaattacac cataaaagga agagggggcc
     5341 ctaattaagt tgtcaagcat gtttatgata cctgcagggt ataaattttc tggctgcaag
     5401 cagacacata taaaacgtct ttattgatcc tacccacata atatggacac agtcgtaagt
     5461 gaggctctgg ttttcaaatt gttccggact gagaccacca caccaacggt gccgtcgcca
     5521 gcgattgtaa tcgcactcga tataactaaa cactgttgcc cgcactattt cccgatggat
     5581 aatagtgttc tccgtggaga cattccattt tcagcgaatg aattttcatc tgttgctgta
     5641 tccagtcata cagtgactgc atcagtggcg cggctctggc ttttcttgcc gccagacgct
     5701 gttctgctga acatccccgg acttctgcct cgatggcata cagttcaccg atacgctgca
     5761 gggcttccgt ggtgatgtcg gttggcactc ttgcatgcac atcgtggatt tttctccgga
     5821 catgagccat acatgcggct tccgttattc tgccggattc gtacaacgcc cggtaaccac
     5881 cgtaagcatc ggcctgaagc actccgctgt aaccggccag atgattctgt ggatggatac
     5941 ctttccggtc cggtgagtac gcgaaccaga ccgccggagg catttccgaa ccggcgttac
     6001 ggtcatcacg aacgtagacc cacagccggg cagtccttgt tttaccgctg cccggctcct
     6061 ggaccggaac ggggatatca tcggcatgga ctttaccggg catcagcaca tactggcgca
     6121 ggatgtcata cagcggttcc agcagttcag caacagcacc tgtccagcgc cccagtgtgg
     6181 cacggctcag ctccacgccc tgacgacggt atatttctga ctggcggtat aacggcagat
     6241 agtctgcata ttttccagca acaacatggg ccagaagccc cgctccggca taactgcgtg
     6301 caatgggttt tgaaggcacc ggtgcctgca cgatatggtc acaccggcaa caggccagtt
     6361 tcggacgttg tgtttcgata accttaaagg cgctgctgat aagctccagc tgctctgaca
     6421 catcacatcc cagagaactg agttcgccac cacaggccgg gcagcattcc tcttccggac
     6481 ggatgacccg ggtttcacgg ggaagtgagg caggtaacgg tttacgggct gaagactggc
     6541 gcaggggaga tggcagtacc gggtcatatt gctcacccag cgtttccgcc atttcttcct
     6601 gaagtgcgct gattcgctcc tgtgcttcca gtatctgccg ttcggttttt gcgcgaagtt
     6661 tttctgagct tttaccgaac tgcatgcgct gcagtttcgc aaccagcgcc ttcagccggt
     6721 tgatttcgga agcataagcc gccacccgct gtgagagcag gcggttgtat ttcgccatct
     6781 ggcggatggt gtcctgttgc gtctgcaaca gtgcccgcag gcgggcattc tcatgagcaa
     6841 gtgaggtgtc catatcctca ctttacaacg ggttatatgc ggatgccagc gcgttccgtt
     6901 cgtttcgggt gtttccagtc gatgccctca agaagcatgg ataactgggc cggagtaagg
     6961 tgcaccttgc cgtcacgggt gaccggccag acgaagcggc cccgctccag gcgtctggta
     7021 aagaggcaca gtccgtcact gtcagcccac aacactttta tctggtcacc ccggcgtccg
     7081 cggaagatga acagatgccc ggagaacggg tcatccttca ggacgttctg aacttttgag
     7141 accaggccgt taaagccatt tcgcatatcg gtgatacctg caaccagcca gatacgcgaa
     7201 cctgcaggga aagatatcat cagtggctgc tcccttttat ttcgcggata agtatctgta
     7261 ataacgccgg cgtcagttta cctttaagcc tgagagttcc ggccggcaga accagctcac
     7321 agcacagact gtcggacggt gtatttatct gctctggttc ctgtgccgga gccgggattt
     7381 tattatccgg ctccggcgtt aacgtcacgg gaagcagtgc aggcatattt tttccggaag
     7441 gcagcaggcc acctttccgg tattgatggc gccagttgaa gagcaggtta tcgttgattc
     7501 cgttttcacg ggcgatctgc gccacacagg ctccgggctg cagtgactgc tccactaaag
     7561 cgattttaaa ttcataagga aaattaggcc gc
//
LOCUS       DAJTSA010000153         7588 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000153 DAJTSA010000000
VERSION     DAJTSA010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7588
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.153"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..137
                     /locus_tag="OO369_003792"
                     /pseudo
     CDS             1..137
                     /locus_tag="OO369_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(167..3079)
                     /locus_tag="OO369_003793"
     CDS             complement(167..3079)
                     /locus_tag="OO369_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983893.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3082..3198
                     /locus_tag="OO369_003794"
     CDS             3082..3198
                     /locus_tag="OO369_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5983894.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3562..4404
                     /locus_tag="OO369_003795"
     CDS             3562..4404
                     /locus_tag="OO369_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5983895.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4407..5495
                     /gene="capU"
                     /locus_tag="OO369_003796"
     CDS             4407..5495
                     /gene="capU"
                     /locus_tag="OO369_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5983896.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5500..6450
                     /gene="virK"
                     /locus_tag="OO369_003797"
     CDS             5500..6450
                     /gene="virK"
                     /locus_tag="OO369_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR5983897.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6515..7459
                     /gene="lpxM"
                     /locus_tag="OO369_003798"
                     /gene_synonym="msbB"
     CDS             6515..7459
                     /gene="lpxM"
                     /locus_tag="OO369_003798"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5983898.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2050 a         1683 c         1780 g         2075 t
ORIGIN      
        1 cctcccggaa gttcagcccg gtcagttacc gcgcacatgg cctgcccgtg tcagaaaatc
       61 tgctggagca ggatttttac gccagtggtc cgaaccagaa gtgggcggga gacatcacgt
      121 acttacgtac agatgaagtt cggttacatc ctgtcagcac ggaacctcac gctttttgag
      181 cggcacgacg gatccccccc acccagtatc cgacagactt ttcactgcgt tcgtaaaaac
      241 ccttcttccc tcccagcata tccactaatt ccggtgacat atcctgcaga taagtctgca
      301 aatcatgccc cagatcttcc ggtgactgca actcataaac cgcgcgccac acatcaccct
      361 tccttatttt aagccccggg ttaagaatat actgtccgtg gttcagcctc agaaaaagct
      421 tttttccata aggatttttg ctgttaactt catgttgaga gagctggctg ctgatataac
      481 tccttcgttt gcggcgttcc ggcagcaacg cttcaggaag atgaaccaca cactctgcca
      541 gtcgtgccgc actgaaggct tccgaaaaaa acagagcact tcccagatgc gtgtggaaca
      601 gcgcctgcat caggatcacc agaaacattg ccatctgccg gttgtccagt ttgatgaggc
      661 gtccttccac ctgcagtgac agactttcag gtttcagcag ggtatacaac tgcgcacttt
      721 tctgttgcgc ataacggggc gtactgaccg ccgctgacag aacttgatga taagccgcca
      781 gcccgtcatt accggtcagc aagggatctg caccacgctc cagcagtaac tgaaccagtg
      841 caatattacc tgcccgggct gctgacatca gcggagtaag attaaacggc gaacggtgat
      901 caacaccata acgttcaata tcccgcaaca cggcaaccgg gttattcatt gagtatggcc
      961 ggaaatactt cagctccagt aactgcagag tcttcgttct gggctgcctt gtgggcttaa
     1021 aatcttcccg gtacagtgca ttatacagcg ggaacaggga ataaatcaga ctgtactcca
     1081 gcgcctgcag ttgtatggtt ttatcggctg tacctgcgag gatatgctgt ttccactgtc
     1141 gtgcatcctc tgcggtgata atctgccatg gcatctcaga tgtctgcagg atccggctac
     1201 ggatggtgtc cgcctgctcc tgcttcccct gtttttcaag cagatgtgcg gttttctgcc
     1261 actcatcacg gctggaaatc tcttcctgca gattcagagg ctcttcctga tgcgtgagcg
     1321 caagcagact ccacagagga tgctccacct gctgttcaac aagatacaca ttgtgtttag
     1381 cacgggtgag cgcaacatac agcgcgttgg taaaaaattt atagatttcc gctgaacgat
     1441 cctgcctgtc ccgcggacgg gaataacggg cctcgccctc aagatcagcc ggtgtaagac
     1501 cctcgcaaat atcatcaaat gcctgccggg cagcgcttac tatattgtaa aggatcaccg
     1561 tttcgtattc cagcccctta acctcctgaa ccgaaaagac cagcggcgta ctgaaccagc
     1621 atcgcgcctg tgctttctgt tccggatgca tcacaatgac cgctacacga ttcgaaagtg
     1681 aggttttggc attcagttcc tgctttgtct cttccctatc gtccagcaac cggatggtcc
     1741 cttctgcctg accgcagctt ctgacaaaat ggtgactttc ccgatcaata gcactaaagc
     1801 ggacctgttt gagtctcagc aaccggttag ccagtgcagt gacgtggtgc ccgttacggt
     1861 aattcgcctg caggatatta acggttgtgt cgtttccctg ttgctgccgg aaaaagagac
     1921 ttttgagcga actccatgag aaaaagctcg gatgaactat ctgattggca tcaccacaga
     1981 gtaaaaactg accgggatga cgtagcgttt tcagtaccag ttgcagttga acaggcgtca
     2041 tatcctgaac ttcatcaaca aaaatgacat cataacgggg agttgccaac gaaagatact
     2101 gatgactgag cagattactg tcatactgat gcgactgttt cagccatgcg atataacgtt
     2161 caaacaggac atagaccgtc gggcggtcct ccatgccata gagcgactgc cgtatcccca
     2221 gtgacagata cgcctcccgg gacagcggcc cattcccgga ggcaaccgca ccgatcacgc
     2281 ccctgaactc ctcatacagg gtatgggcag cccccagtgc ggcgatagcc ctgttgcggg
     2341 gcagccagtc gctgaacgca ctgaaagtca cctccctgcc ttccggaatg cgcagagttt
     2401 ccagaaattc cgtcagcgaa agaaagtcaa tattctgtac ctcactccct tcccctgaag
     2461 cgtcatagag agtacgcgct ttttccacca gaaatgagga aaggctgaga tacagtatat
     2521 caccagcagc ctgcttcatt ttttccagta ccagcgccgt cttaccactt cccgccgagc
     2581 caacaatcac cagcggtagc gggtgttcgt acagtgcctg ctgatcatca tcaaaggaca
     2641 gcattttatc caggcggtgg aaacgcccat gagacggatt gatatacgtc agcgcttcag
     2701 tcgcgatatc aacaggatcc ggcactggtt gttgttgtaa gcgcccttca tcaatcacca
     2761 cgtttctgcg aagaaaacgt gaagtgttat aggcatgatt gcgaatatat tccagaacaa
     2821 ggcagatagt ttcaccgtgg tagcgataca gcgaaaacag taatcggtcc cggatactga
     2881 gccgggcgcg gtaaagattg gtgtcaattt tacgaacatc agcctgaaca aagttatcgg
     2941 tggcaatagc ctgtgcaaat tttttataac ccgggatcgt gtcaggattc agagtatgat
     3001 aaagacagat tttcatggct gccggtttcc gtcgtgcaaa aaacatgctg attttacgaa
     3061 ggaatgcctt actgaacaac cttgcccgga ataccccccg aaatcagatg gaacaatcag
     3121 aaaaatgctc tgcagttatg gaataaagaa ggtggggtgg tacgccggga gggcgatccg
     3181 ctggaaatgg cgcagtagct gcaggcctgt catgatgcag gaatagaagt tcgtgtacag
     3241 gttaatgaaa gcgtcacccc gtaaataaac tctgcgctga aattcctttt tttcattgga
     3301 gtacctgtgt tgttctgagg tgaacatatc acctctgttc aggtagccaa attcagtgtg
     3361 ccacctcaag gttcttagtg agtcgttttt aaaagatatg ataatatatc attgtttatg
     3421 aattgttgat gtttaattta gtaaattata tcaaatatgc catgggtttg ctatgttatt
     3481 catccagtat ctggaaataa aatgcaccag ctttattggg ttttcctttt gtctctgctt
     3541 gctaggtgcc agaaacttta aatgttaaac gaagggggca ttttgttcaa agccaatcat
     3601 gttccggtat taatgtatca tcatgtcagt cattgccccg gactggttac actttctccc
     3661 gtgacattcc ggaagcaaat aaaatggttg gctgaaaaca actggaaaac actgtcatca
     3721 gatgaactgg agttttttta tcggggcgga aaactccccc ggaaaagcgt tatgttgaca
     3781 tttgatgatg gataccttga caactggttt caggtatatc ctttattaaa agagtttaat
     3841 ctcaaagcac atattttttt aattaccggt tttattggga atggtccggt tcgtcactct
     3901 cctggaaagg aatattctca tcgcgattgc gaacatcaaa tagcgacagg gaatgctgat
     3961 aatgttatgc ttcgctggtc tgaagtaaac gaaatgttgc aatctggtct ggtcgaattt
     4021 catgttcata cgcatactca tacacgctgg gataaaaagt tcagttcacg agaagaacag
     4081 tgtaaacatc ttcggcagga cttgttgagc gggagggaat atttgaaaga aatgacagga
     4141 aaatgcagta aacatttgtg ttggccggaa ggttattata acaaggatta tattcaggtc
     4201 gctgaagaac ttggattcta ttatttatat acaacagaaa gaagaatgaa tgctcctgct
     4261 aaaggaacta cacggatagg aagaataagc accaaagaac gggaaagttg cgcatggctg
     4321 aaaagacgac ttttttatta tacaaccccg tttttttcat cgttgctggc ctttcataag
     4381 gggccaagat taccggatga ttaataatga atatactatt tacggaatca tcaccgaata
     4441 taggtggtca ggaattacag gctgttgctc aaatgaaggc cctgaagaaa atggggcatt
     4501 cagttctgct tgtctgcagg gaaaacagca aaattgcttt tgaagccagt aaattgggga
     4561 ttgatatcac attcgcgtta tttcgaaaca gtcttcacat ccctactgca tggagattac
     4621 tcggaatagt tcatggtttt cagcccaatg ccatcgtttg tcacagtggg catgatagca
     4681 atattgttgg tttagtacgg ttattcacca ggaaacatcc attcagaatt atcaggcaaa
     4741 agacatattt gacacgaaaa acaaaagttt tttcaataaa tcatttttgc gatgaggtga
     4801 ttgttcccgg aacaagtatg aagacacatc tggagcagga aggatgtcga acccgggtta
     4861 ctgttgtgcc tccaggcttt gatttccaga aattatacgt tgattcccga aacagtttgc
     4921 ctccaaatgt tctttcttgg ctggcgtccc gaaggggatg ccccgttatt gctcaggtag
     4981 gaatgttgcg cccggaaaaa gggcacgaat ttatgttgaa tttactgttc catttaaaaa
     5041 tgaatggacg acagttctgt tggttgattg tggggtctgg ttcgcctgaa ctgcgggagc
     5101 atttacagta tcagattgac agtatgggca tgcatgatga tgtttttatt gctgacaatg
     5161 tttttcctgc cgcccccgta tatcgggttg ccagtctggt ggttctgcct tcagaaaacg
     5221 aatcgtttgg tatggtactg gcagaagcat cggcattttc tgtgcctgta ctggccagtc
     5281 agattggtgg aatccctgat gttattcaga ataaccagac cgggacattg ttaccagcag
     5341 gtaataagca cgcatggatg tgcgccctga atgatttttt taatgaccct gggcgttttt
     5401 atcagatggc tcgccaggca aaacaggata tagaagagcg gtttgatatt aataaaactg
     5461 cgttaaaaat actcacatta gcgaagcaca agtaacaata tgttttctgt aagtaactta
     5521 tcatttatcg gtttccttaa aaggattgtt ttttcctcag attcactccc ggggaagtgg
     5581 gaacacagaa aattccggtt catgtacatt ttgcgatgtg ctataaatcc ggttgccagt
     5641 attcgatatt attacgaact gcgttccttg cagtgcattg aggatattct ggcaatacaa
     5701 cccacgttgc cagcaagaat tcatcgacct tatttgcata aaggggggcg agcctggtcc
     5761 cgggggcaat atattcttga gcattatcgc tttgtgcaaa atttgccaga aaaatattct
     5821 gaatttttgt ttccacaaaa atcggtatca ttagtccaat tcatcgggaa agatggagag
     5881 gattttgata tccagtgttc tcccagtggt tttgaccggg aaggagagtt gatgttgtca
     5941 ttgtttttca ataaaatagt gattgcccgg ttaacatttt cagtcattct gactcaaaat
     6001 ggacacaccg cttttattgg cggattacag ggagccccca aaaataccgg acctgatatt
     6061 attcggtgcg caacccgggc ctgttatggg cttttcccaa aacggattat ttttgaagca
     6121 ttttgtgcgt tgatgaaagc ttgtaatgtc tctgagtgtc tggcagtcag tgagcacagt
     6181 catgttttcc ggcaattgag atactggtat cagaaacgca aaacctttgt tgctgtctac
     6241 agtgatttct gggagtctgt agcagggaaa acctgtggtg actggtacaa attaccaaca
     6301 caggtggttc gaaagccgct aagcaatata gctagtaaaa aacgctctga gtatcgaaaa
     6361 cgatacgctt tgctggatta tatccatgaa actgctatcc gctctttgga tgcatatcct
     6421 gtaaactcag aacatcagga cttaaattaa ggtttgatgt attctctgaa taaaatatta
     6481 atgatgattt tggtaggggc attcgcacta aataatgaaa aaatacaaat ccgagtttat
     6541 tcctgaattt aagaaaaatt atctttcccc tgtttactgg tttacatggt tcgttttggg
     6601 aatgattgca ggtatttcaa tgtttccccc ttcattcaga gatcctgtct tggccaaaat
     6661 agggcgttgg gtgggcagat tgagcagaaa agctcgtcgc agggcgacga ttaatttatc
     6721 gctttgtttc ccggaaaaga gtgatacaga acgggaaata attgtcgaca atatgtttgc
     6781 cacagcattg caatccatag tgatgatggc agaactggca attcgtggtc cggaaaagtt
     6841 ccagaaacga gtgttctgga aaggcctcga aattcttgag gaaattcggc acaataacag
     6901 aaatgtgatt tttctggttc cccatggctg gagcgtggat attcctgcaa tgttgctggc
     6961 agctcagggg gaaaaaatgg ccgccatgtt tcatcagcaa cgaaatccag tgattgatta
     7021 tgtctggaat tctgtacggc gtaaattcgg ggggcgctta cattcccggg aggatgggat
     7081 aaaaccattt attcagtcag tacgccaggg atactggggg tattaccttc cagatcagga
     7141 tcatggtcct gaatacagtg aatttgctga tttttttgcg acctataaag cgacattacc
     7201 aattattgga cgtctgatga acatcagtca ggctatgatt ataccgcttt tcccggttta
     7261 tgatgaaaaa aaacatttcc tgactattga agttcggcca ccaatggatg catgcattgc
     7321 cagcgcggac aataaaatga ttgcccgaca aatgaacaaa acagtggaaa ttttggtggg
     7381 gtcacatccg gaacagtata tctgggtttt aaaattgtta aaaacgcgca aatcaaacga
     7441 agcggacccg tacccttgaa attataaagt acaggtattt ccactagttg tttcttacag
     7501 gttaccaatc gaaacacatc ccccttccgt gtgttgcccg tttggcacgg aggactgtgt
     7561 agatgcagtt cgttaaattt aatttatg
//
LOCUS       DAJTSA010000154         7537 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000154 DAJTSA010000000
VERSION     DAJTSA010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.154"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1158
                     /gene="rbsA"
                     /locus_tag="OO369_003799"
                     /pseudo
     CDS             1..1158
                     /gene="rbsA"
                     /locus_tag="OO369_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1163..2128
                     /gene="rbsC"
                     /locus_tag="OO369_003800"
     CDS             1163..2128
                     /gene="rbsC"
                     /locus_tag="OO369_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR5983899.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2153..3043
                     /gene="rbsB"
                     /locus_tag="OO369_003801"
                     /pseudo
     CDS             2153..3043
                     /gene="rbsB"
                     /locus_tag="OO369_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3169..4098
                     /gene="rbsK"
                     /locus_tag="OO369_003802"
     CDS             3169..4098
                     /gene="rbsK"
                     /locus_tag="OO369_003802"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR5983900.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4102..5094
                     /gene="rbsR"
                     /locus_tag="OO369_003803"
     CDS             4102..5094
                     /gene="rbsR"
                     /locus_tag="OO369_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR5983901.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="OO369_003804"
     CDS             complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="OO369_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR5983902.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6510..7202)
                     /locus_tag="OO369_003805"
     CDS             complement(6510..7202)
                     /locus_tag="OO369_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR5983903.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1916 a         1828 c         1986 g         1807 t
ORIGIN      
        1 aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct ttaaaagcga
       61 caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg ccaaagtgct
      121 gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga
      181 aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccact gtattgtcta
      241 tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg tttttcgtga
      301 tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc tgattgagat
      361 gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc caggagatat
      421 ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt ttactttacg
      481 caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg aactgatgaa
      541 agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg ggcatgaagt
      601 cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg
      661 taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct
      721 gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggctgtgag
      781 tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa ttggtctgct
      841 ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac ccccaaagta
      901 ttgatccttg atgagcctac ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa
      961 ctgattaacc agttcaaagc cgatggcttg agtatcattc tggtgtcatc ggagatgcca
     1021 gaagtattag gcatgagcga tcgcatcatc gtcatgcatg aagggcatct cagcggggaa
     1081 tttactcgtg agcaggccac ccaggaagtg ttaatggctg tcgctgtggg caagcttaat
     1141 cgcgtgaatc aggagtaaaa aaatgataac ccagactgtc tctggtcgcc gttatttcac
     1201 gaaagcgtgg ctgatggagc agaaatcgct tatcgctctg ctggtgctga tcgcgattgt
     1261 ctcgacgtta agcccgaact ttttcaccat caataactta ttcaatattc tccagcaaac
     1321 ttcggtgaac gccattatgg cggtcgggat gacgctggtg atcctgacgt cgggcataga
     1381 cttgtcggta ggttctctgt tggcgctaac cggcgcagtt gctgcatcta tcgtcggcat
     1441 tgaagtcaat gcgctggtgg ctgtcgctgc tgctctcacg ttaggtgccg caattggtgc
     1501 ggtaaccggg gtgattgtag cgaaaggtcg cgtccaggcg tttatcgcta cgctggttat
     1561 gatgctttta ctgcgcggcg tgaccatggt ttataccaac ggtagcccag tgaataccgg
     1621 ctttactgag aacgccgatc tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc
     1681 gacgccagtc tggatcatgg ggattgtctt cctcgcggcc tggtacatgc tgcatcacac
     1741 gcgtctgggg cgttacatct acgcgctggg cggcaacgaa gcggcaacgc gtctttctgg
     1801 tatcaacgtc aataaaatca aaatcatcgt ctattctctt tgtggtctgc tggcatcgct
     1861 ggccgggatc attgaagtgg cgcgtctctc ctctgcacaa cccacggcgg ggactggcta
     1921 tgagctggat gctattgctg cggtggttct gggcggtacg agtctggcgg gcggaaaagg
     1981 tcgcattgtt gggacgttga tcggcgcatt aattcttggc ttccttaata atggattgaa
     2041 tttgttaggt gtttcctcct attaccagat gatcgtcaaa gcggtggtga ttttgctggc
     2101 ggtgctggta gacaacaaaa agcagtaata acgactacag gacatcttga atatgaacat
     2161 gaaaaaactg gctaccctgg tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc
     2221 gatggcaaaa gacaccatcg cgctggtggt ctccacgctt aacaacccgt tctttgtatc
     2281 gctgaaagat ggcgcgcaga aagaggcgga taaacttggc tataacctgg tggtgctgga
     2341 ctcccagaac aacccggcga aagagctggc gaacgtgcag gacttaaccg ttcgcggcac
     2401 caaaattctg ctgattaacc cgaccgactc cgacgcagtg ggtaatgctg tgaagatggc
     2461 taactaggcg aacattccgg ttatcactct tgaccgccag gcaacgaaag gtgaagtggt
     2521 gagccacatt gcatctgata acgtactggg cggcaaaatc gccggtgatt acatcgcgaa
     2581 gaaagcgggt gaaggtgcca aagttatcga gctgcaaggc attgccggta catccgcagc
     2641 ccgtgaacgt ggcgaaggct tccagctggc cgttgctgct cacaagttta atgttcttgc
     2701 cagccagcca gcagattttg atcgcaccaa aggtttgaac gtaatgcaga acctgttgac
     2761 cgctcatccg gatgttcagg ctgtattcgc gcagaatgat gaaatggcgc tgggcgcgct
     2821 gcgcgcactg caaactgccg gtaaatcgga tgtgatggtc gtcggatttg acggtacacc
     2881 ggatggcgaa aaagcggtga atgatggcaa actagcagcg actatcgctc agctacccga
     2941 tcagattggc gcgaaaggcg ttgaaaccgc agataaagtg ctgaaaggcg agaaagttca
     3001 ggctaagtat ccggttgatc tgaaactggt tgttaagcag tagttttaat caggttgtat
     3061 gacctgatgg tgacataaat acgtcatcga cagatgaacg tgtaatataa agaaaagcag
     3121 ggcacgcgcc accctaacac ggtggcgcat tttatggaca tcccgaatat gcaaaacgca
     3181 ggcagcctcg ttgttcttgg cagcattaat gctgaccaca ttcttaatct tcaatctttt
     3241 cctactccag gcgaaaccgt aaccggtaac cactatcagg ttgcatttgg cggcaaaggc
     3301 gcgaatcagg ctgtggctgc tgggcgtagc ggtgcgaata tcgcgtttat tgcctgtact
     3361 ggcgatgaca gcattggcga gagcgttcgc cagcagctcg ccactgataa catcgatatt
     3421 actccggtca gcgtgatcaa aggcgaatca acaggtgtgg cgctgatttt tgttaatggc
     3481 gaaggtgaga atgtcatcgg tattcatgcc ggcgctaatg ctgctctttc cccatcgctg
     3541 gtggaagcgc aacgtgagcg tattgccaac gcgtcagcgt tattaatgca gctggaatca
     3601 ccactcgaaa gtgtgatggc agcggcgaaa atcgcccatc aaaataagac tatcgttgcg
     3661 cttaacccgg ctccggctcg cgaacttcct gacgaactgc tggcgctggt ggacattatt
     3721 acgccaaacg aaacggaagc agaaaagctc accggtattc gtgttgaaaa tgatgaagat
     3781 gcagcgaagg cggcgcaggt actgcatgaa aagggtatcc gtactgtact gattacttta
     3841 ggaagtcgtg gtgtatgggc tagcgtgaat ggtgaaggtc agcgcgttcc tggattccgg
     3901 gtgcaggctg tcgataccat tgctgccgga gataccttta acggcgcgtt aatcacggcg
     3961 ttgctggaag aaaaaccatt gccagaggcg attcggtttg cccatgctgc cgctgcgatt
     4021 gccgtaacac gtaaaggcgc acaaccttcc gtaccgtggc gtgaagagat cgacgcattt
     4081 ttagacaggc agaggtgacg cttggctaca atgaaagatg ttgcccgttt ggcgggcgtt
     4141 tctacctcaa cagtttctca cgttatcaat aaagatcgct tcgtcagtga agcgattacc
     4201 gccaaagttg aagcggcgat taaagaactc aattacgcgc catcagctct ggcgcgtagc
     4261 ctcaaactca atcaaacaca taccattggc atgttgatca ctgccagtac caatcctttc
     4321 tattcagaac tggtgcgtgg cgttgaacgc agctgcttcg aacgcggtta tagtctcgtc
     4381 ctttgcaata ccgaaggcga tgaacagcgg atgaatcgca atctggaaac gctgatgcaa
     4441 aaacgcgttg atggcttact gttactgtgt accgaaacgc atcagccttc gcgtgaaatc
     4501 atgcaacgtt atccgacagt gcctaccgtg atgatggact gggctccgtt cgatggcgac
     4561 agcgatctta ttcaggataa ctcgttgctg ggcggagact tagcaacgca atatctgatc
     4621 gataaaggtc atacccgtat cgcctgtatt accggtccgc tggataaaac tccggcgcgc
     4681 ctgcggttgg aaggttatcg ggcggcgatg aaacgtgcgg gtctcaacat tcctgatggc
     4741 tatgaagtca ctggtgattt tgaatttaac ggcgggtttg acgccatgcg ccaactgtta
     4801 tcacatccgc tgcgtcctca ggccgtcttt accggaaatg acgctatggc tgttggcgtt
     4861 taccaggcgt tatatcaggc agagttacag gttccgcagg atatcgcggt gattggctat
     4921 gacgatatcg aactggcaag ctttatgacg ccaccattaa ccactatcca ccaaccgaaa
     4981 gatgaactgg gggagctggc gattgatgta ctcatccatc ggataaccca gccgaccctt
     5041 cagcaacaac gattacaact tactccgatt ctgatggaac gcggttcggc atagatttac
     5101 gctgtctttt gatcaaatta ttaccatcgg ttgttttcag aagcatgaac attgctgctg
     5161 aagcaacagt aataatgccc atcgtgataa acgtatagtg gaattgttcg acagtcgttg
     5221 tgccttccat cccttcataa atgcgaagga cggccgcact tacagcaacg cctaaactaa
     5281 tcgacagttg ctgcgtgacc gccagaacac tgttaccgct gctggcgttg tcatcggtca
     5341 gatcggcaag tgtgatggta ttcatcgcgg taaactgcgt cgacatagcc atccctaata
     5401 taaacaacgg caagatcagc atccatatcg ccattgccgg tgattgcaaa gagaactgag
     5461 cgatcattag cccaataatc accgtgatcc ccactaatgt atggcgatag cccagacgac
     5521 gtaagacttg ggtaaccatc gattttgcaa taatggaacc taacgctgtc ggtgccatca
     5581 tacagccggc aataaacgcc tgataaccaa atcctacctg taacatcaat ggcataagga
     5641 acggtacaca gccggtcccc agacgggttg caatattgcc tacgataccg atcgagaaag
     5701 tgcgggtttt aaataaatct aatgaaatta atgggtttgg cgtacgtcgc gcatggagaa
     5761 tatagagaag cagtaacccg atgctggtga caattaccgt caaggcaatc cagctggcga
     5821 caatcttttc cccgaatagc tctattccgc ttgaaaagag aacaaggctg aggccaaaca
     5881 gcaaaaagcc agtgatatcg aatctgcgtc gtgcggtggt gaaattgggc atatgtttgc
     5941 gcgcgtaaag aaggcccgca atacctatgg ggatattaat taaaaatatc cagtgccagg
     6001 ttgcccaggt gaccagcacg ccgccaagaa cggggcctaa aattggcccc accagacccg
     6061 gcatggcgac aaaattcaat accggaagaa gttcattacg aggataagca cgcagtaagg
     6121 ccggccgagc aacaggcatc atcattgcgt cgcctatccc ttgaataacc cggaagacaa
     6181 ccagctgtgg tagcgaatta gaaagtgcgc aggccagaga acccaacgtg aacagactca
     6241 cggcaagggt aaaaatgcga cgcgtaccga agcgatcggc tagccatccg cttaccggaa
     6301 taagcatcgc caccgtcagc gtataactga tgatggctga ttgcatcgcg agaggagaac
     6361 gattaaggct atgagcgatt gcgggtaagg cggtattcag aatagtggca tcaagtgcct
     6421 gcatgaagaa ggccatcgcc gcgatccacg gcaaacccga catactacgc ttctttttat
     6481 cgctcattca atgtcctgtt atcgggttat cacttatcag gtgagcgtag cagcgcctga
     6541 caagctttaa atgccgcgtc gccatcgctt tggataatcg catcgacaat cgcctggtgc
     6601 agatccagct ttatcactgt gtcgctggta attgacgtga agtaagtgtg ataaaccgaa
     6661 tggaatagcg aggcgaatga ggtcaaaaac ggattggcgc tcatttcata gatatgctca
     6721 tgccaggcca tatcgacttc gatccagcgt tcacggcgaa agttctcttt taatgccgcc
     6781 atttcggcca ttaacgtatt gagatgcgcc ttctgttccg cggtgccaac cgttgctgcc
     6841 agtaggcagg cttgcggctc cagacagata cgcataacca gaaagtgatc gatgacctga
     6901 tgaaagttct cttctgttat ccaccaagta agcaattcct gatcaagaaa attccagttc
     6961 gattgtggca tgacccgagt accaattcgc ggtcgcggta aaaccatccc ttttgccgtt
     7021 aacgttttga ccgcttcgcg taccgctgta cgactcactc caaattgctc gcccagctca
     7081 atctcaccgg gcaaaatggt gccgggttca tattcacctt ttaagatccg ttgcgccagc
     7141 ttctcagcca gaacatacga aaggtttttc tgtgcagcta actgttgtgc gcttaaaggc
     7201 attacttatc ttcctttttc tttttattcc tccttagtat gccaccagga agtgtgatta
     7261 cggttgcaaa aacggcaaat tgcttgtttt atggcacatt aacggggtgt tttgctgaaa
     7321 aaatgcgcgg tcagaaaatt attttaaatt tcctcttgtc aggccgggat aactccctat
     7381 aatacgccac cactgacacg gaacaacggc aaatacgccg ccgggtcagc ggggttctcc
     7441 tgagaattcc gacagaaaaa gcaaaaataa atgattgact ctgtagcggg aaagcgtatt
     7501 atgcacaccc cccccgcgcc gctgagaaaa agcgaag
//
LOCUS       DAJTSA010000155         7455 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000155 DAJTSA010000000
VERSION     DAJTSA010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7455
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.155"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..694
                     /locus_tag="OO369_003806"
     CDS             <1..694
                     /locus_tag="OO369_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5983904.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIA"
     gene            complement(1033..1803)
                     /locus_tag="OO369_003807"
     CDS             complement(1033..1803)
                     /locus_tag="OO369_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983905.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1848..2282)
                     /locus_tag="OO369_003808"
                     /pseudo
     CDS             complement(1848..2282)
                     /locus_tag="OO369_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2294..2515)
                     /locus_tag="OO369_003809"
     CDS             complement(2294..2515)
                     /locus_tag="OO369_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983906.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2516..3199)
                     /locus_tag="OO369_003810"
     CDS             complement(2516..3199)
                     /locus_tag="OO369_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR5983907.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3263..3574)
                     /locus_tag="OO369_003811"
     CDS             complement(3263..3574)
                     /locus_tag="OO369_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983908.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3571..4473)
                     /locus_tag="OO369_003812"
     CDS             complement(3571..4473)
                     /locus_tag="OO369_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCR5983909.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4913..5872
                     /locus_tag="OO369_003813"
     CDS             4913..5872
                     /locus_tag="OO369_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCR5983910.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5872..6267
                     /locus_tag="OO369_003814"
     CDS             5872..6267
                     /locus_tag="OO369_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR5983911.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6450..6830
                     /locus_tag="OO369_003815"
     CDS             6450..6830
                     /locus_tag="OO369_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR5983912.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
     gene            6876..7455
                     /locus_tag="OO369_003816"
                     /pseudo
     CDS             6876..7455
                     /locus_tag="OO369_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT         2022 a         1930 c         1820 g         1683 t
ORIGIN      
        1 tgatgcagaa gccatatgtg aagcggcttc acgaccatca atgcgttttg tccagccaag
       61 aactgagacc cagcaagcaa taagggcatt acaccgggtc agagaatcgc tcatcagaga
      121 taaggtgaaa acaaccaacc agatccacgg tttcctgctg gaatttggaa tcagcctgcc
      181 gacaggggat gccgtaataa aacggttgtc tctcgtattg gctgaacacg aaatccccga
      241 atatctgagc cgccttttgg tgaggttgca tacccactac ctttaccttg ttgagcaaat
      301 agcagagctg gaatcagaat taagtcagtc aatcaacgct gatgataccg ctcagcgcat
      361 tatgacaatt cctggtgttg ggccgattac tgccagctta ctatcgtcgc aacttggtga
      421 cgggaaacag ttttcatgca gcagagattt tgcggcttcc acagggctcg ttccccggca
      481 gtatagcact ggaggaaaat cgactctgtt gggtatcagt aagcgaggtg ataaaaattt
      541 acgccggtta ctggttcagt gtgcccgctc attcatgatg cagcttgaac gccagcatgg
      601 aaaacttgct gagtgggttc gggagcaact caacaagaag cattcgaatg tggttgcctg
      661 cgctctggca aataaactgg cccgaattgc ctaggcaatc actactcagc aaaaagaata
      721 tcaggcctga tatatagatg ctgacaaaac tcaattagct tattgcaaac aataagatac
      781 aagttaccgt ctggttttgc gaagattgat tattgatgac atgaacggca aatcgacctg
      841 attaaaaacc taacagggga aaatggctca ctgaagccga ggggattttt aggattatca
      901 ggtgcggaac tcatcgaggc gcgggcatcc cttgcccaca gagacgccgg atagatttaa
      961 gcaagcccac cattaatcaa aaatcagtgt tgcaaaaacg ggggtgacca tagattttcc
     1021 ccgccatcgt catcatttcc ggatttttgc cagggcatcc gccatgctgc ggatttcggc
     1081 ccgggcttcc cggacaactt cccgcagaac accacggtca aaccaccggc ggggcggttc
     1141 atccggtata cgcagcacat cgcaggccag cccactggac aggatatgct caatacaatc
     1201 gctgaccaga tacatttcca ccgccgaaaa acgtgcgccc cggttcagga tggcatcgag
     1261 caccacgggc aggttttcat cgtcttcatc gagccagcag gggtgatcgc tttccttcac
     1321 gaccaggcga aagcggcgcg tctggcgggt cagcaacgtc tgaccgcttg tggtcagcgc
     1381 cgcccggata cgccgcgcat cttctgcccc ccggcaactg acactgcata ctgcatcgcg
     1441 caccgcatca agaactgccc tgatttcgtc ttcggtcagc cattcaccgg catacgggaa
     1501 agacacctgc gttccgtccg ccggaagtac cggcagcgac tgccactgac tgccggacag
     1561 aacaagttcc gctccggttc cggtcagttt ctgaatggcg gaggccaggt gcggcggcag
     1621 cacgccatcg tggcggttaa cgagtaacgt cgccagccgg ggcgcatcca gccgcacgta
     1681 aatgctgatc cagcccggat agttcgggtt acattggtca acatccgtct cttccggcca
     1741 gccctgcgcc agcagcaggt tacgggcgtg acgacaaatc agagcattca gcgtttcatt
     1801 cataattcat gaactccctg tatcgccgcc cacaggcggc gataacatca gtcgttttcg
     1861 tgtgtgttgt ttttcagcag gctgcgggcc tgatcaacca tcgcctgtcg gcgggtaacg
     1921 tattccggga gggggaaacc gtgatcccgg gcagcccatt cacgtttcca cagtgcctgt
     1981 tcagcgatct gagtcagggt ctccagcagt tcagcaggga cggtcacctg gcggttttcg
     2041 cgcacgttgc tgataatctc catcgccgcg gcggaagaaa taccggactc aatccgctag
     2101 tcttccggga tgtcctccag gtgcgccagg acggtcctga tttcatgatc ggacagggaa
     2161 atatccattc cgtcagcgag ggcctgaatc tcttccgggg accagataac cagcatcagc
     2221 ggcgtgtctc ccgaatactt tgctgccagc gcccggcata gcgtttcgca tgttccgtac
     2281 atactctctc ccctcaatct ttgaagcagg aatccagttc cagaatttcg gtatatgcct
     2341 ccatcaagag gcggtttgcg cgaaacaacg tctgacgcac cagacgttcc ggcagggtgt
     2401 cagcattaaa gctgtaccgc cgctccatct cattcatggt tgtacgcagg tcgcacatgg
     2461 cattggccac ctccgccact tcggcgcgaa gtttttcgtg tccggcatag ttcatttacg
     2521 cagcctccgg cataaaccag tcatcattcg cggccccctg ctgcatggcc cgctgtacgg
     2581 cagccagtcg ttgctgtccg gcacggtgat actgctcaag cagctcgata ccgatatacc
     2641 ggcgtccgga ctggagggcg gcgacgcagg ttgaaccgct gcctgcaaac gggtccagca
     2701 ctatcgcatt cgggtgtgtg aagctctcaa tcagcggttg caggctggta acgggctttt
     2761 ccgtcgggtg atggcggttg cccgaatatt tccagcccaa cacgtccggc agcgggtttt
     2821 gtggcagacg tggacggcct tttgccagga tgtaggcgca ttcgtggcga tagcccacat
     2881 atgcggcctt cgatgtgtag tttttggtga agaccaggtg accaacaacg ctgaatcccg
     2941 catttttcca ggcagacata aagcgatcga cgcggttcca gccatagaag ctcaccatca
     3001 gcgcgtcttt tttgagtacg cggtacattt cattacaggc cggttgcagc cattcatcag
     3061 ttttatcacc agcaatggta cgcccctgac ggtcacgaaa accgacgaga tatggcggat
     3121 cggtgaggat gaaatcaatg gcgttatcag gaatacgggc cataacatcg atgcagttac
     3181 cgaggacaaa acgggacata acagatcctt ctcttacaga gggcacccct gtggtgcccg
     3241 ttccccgctg cctgtgggct gttcatcgct tccggtaacg ggtcaggccg accgtggaat
     3301 accgcagccg tcgtcttttt gacagcaagg ttatgccgcg aaagcggctt gaagggcgtt
     3361 catcagaaaa gggcgacggg ctggcgtaca ctgtcaccga caccagccgg gagcggtaaa
     3421 agcgcccgtc ccgtgcccgc cggacgcggg acactgacca caggcagcgt ttatgccgcg
     3481 ccgcgtcagc cggcgcagta aaaagaggag cacggcgaca tcctttgtcg gaaaagaaac
     3541 gggcggttgc ccgcccgtct ccgtcatact tcatccgcca taatgcgcca cattgtcgac
     3601 tatccacgca ggtccggtga cttccggctg ctcatcgtca tctgtcgggg aagaccattc
     3661 aagttccccc cacagcacat tgacgagctc tccacgctca tacaactgcc agccacagaa
     3721 atcacagccc tgttcggcat accagtgctc cagcgtgcag ctgaaacggc ggcttaattc
     3781 tgcaataacg tcgctttccg gctgacacca cggagtgtcg aaatccacct gaatgaagtt
     3841 ttctccctga ctgctgatat tcaggtcata tccacaaggc cacttcacgc cataccgttc
     3901 cgtataccag tgatatgcag aaggaacacc gttcagaaca ccaccattaa agccgttcac
     3961 ttcaacatcc aggcgggtcg gcagaaccgt cagcaggtca aacggcatgg ttttttccgg
     4021 cactacgttg tcacacagac ggttccacca caccgataca tcctcgttac cccatatgtc
     4081 acaccagtca cctctttttg cggtgaaatg cacagtgatg gtttccctgg catcatccgg
     4141 taatccttcc caacgacgct ggccagtacc gctctgtaac cagagttcat gcagcatcag
     4201 gcagttttgt tcatccagca acacaccgtt ctgcaggtgt gtcagccagc gggtgaacgc
     4261 gatattttcc gtcgatacag caccacgtcc ggcagccgtc actccggggc actgttcaga
     4321 gcggatattt tcagtgatct gcagaagtcc ggcacttccg gccagaaaca gctgaatacc
     4381 ttcatttgtg gcgcggcgat aaagcggtgt gactgcaccg ctggccagtc gtttaatctc
     4441 agcgatctgt gccggctcac caggaaaata catacgattc gagcaccagt ttggcatggg
     4501 aattctcctc tcacatgcgg cctccatcag gccgtgccgc acctgtaacc gggcgaccgc
     4561 atacgaatgc aggtgcggcc ccgaagccgc agcgcgtgga ggaggaatga cgaaacgtga
     4621 ccgggtgagg aggccggcga cagcgacaca cttgcatcgg atgcagcccg gttaacgtgc
     4681 cggcacggcc tggttaaata gtatccgtct cagttcgtgg cgcaaaatga gaaaccaata
     4741 agaactcaaa atgaaccaca acaagaacat aaagagactc taaaagagca acaccgtttg
     4801 acatagactc aaaatgagtc acaataagaa acaataaaaa actcaaaaag atgcaccgac
     4861 ataaaggatg ctcaatttga gataaacaca aactcaaaac gaggcaaaaa gaatgctaaa
     4921 agtatcatgc gatgatggtt caactaacgt aaaactggcc tggcttgaag atggcgaggt
     4981 aagaaccagt ctgtcaggaa acagctttaa agaaggctgg aacccgggac tgtttaatgc
     5041 cggcaaagtt tacaattacg tcgtggatga aaaaaaatac acctatgatt tgggaagcac
     5101 tgctgtcata ggtacaacac atgtcagtta tcaatacagc accaccaatc tgcttgccat
     5161 acatcatgca ctgctgacat ccggccttca gccacaggat gtagaactga cagtaacatt
     5221 acctgtgacc gaattctttg ataatgacaa tcaaccaaat gaagaaagaa tagaaagaaa
     5281 aaaggcaaat gttctccgtg agatatctct gaataaaggg gaaacattta aaattaaaaa
     5341 agttaatgta atgcctgaat ctttgccagc tgcatttgaa tcactaaaaa aagataaagt
     5401 aaacaaactt gaacgcagcc tgattatcga tcttggagga accactctgg attgtggttt
     5461 aattctcggt gcatttgaag gcatttctga aatccgtggc tattcagaaa tcgggacatc
     5521 ccgcatcact cataccgtca tgaatgcgct gacgaaagcc tccacaccat gtaattattt
     5581 tattgctgat gagttaataa aaaacagaca cgataatgaa tatctccaga cattaataaa
     5641 tgatgtagct gaaataaaaa acatttctca tgtaatagac agagaagtga aatctctggc
     5701 agaaagcatc aggcaggaaa tttcaacatt ctcaggaatg aaccgaatct atctcactgg
     5761 cggaggagcc gagcttattt accctcacat aaagcaatat ttcccgaatc tgaaagtgaa
     5821 caaggtggat gaacctcagt ttgcattggt gaaagcaatg gtgcatgcgt aatggaaagc
     5881 agtgatccaa aaaaaagaaa gaaagttgtg gcatacctgc acccggcact ttatccgcag
     5941 gacaacctga cacaacaaac aatagactct ctcccagtac aaatgcgggg agatttctac
     6001 agacaatccc ttatttgcgg agcagctctt tattctgttg ccccccgact gttaacgcta
     6061 atcagcgttt ttttcagtga gaaaatcact gcagaaaatc tggtgaaact tatagaacaa
     6121 acaacaggat acacatccac atcaattgat ataagtgtgt taaaaaacat catagaggca
     6181 tcttcagaaa acaaatcaga gagcatcact tcaaaagatg atttcgaaga acaaaccaga
     6241 cgcaatcttt ctatgttaaa aaaataataa aagaagagca ggttcagtca attacctgct
     6301 cttcttttat acatactaaa aaatcagatt tttacgaata ttcagattat cataaaaaat
     6361 gcaacagaca ggaatgcaat aattttgcat tatctcgttg cttgtaatgc aaatatgcat
     6421 tattgtcagt gtgtgacaat aacagagaga tgacgatggc gaccattaac gcacgtattg
     6481 atgatgatat aaagaaccaa gcagatgaag tgctgaagct gatgaatatc agtcaaactc
     6541 aggctattgc agctttttat cagtatatta ctgaacaaaa gaaactgcca tttgtgatca
     6601 cctcaattgt aaaaacgccg catgatttat tacgtgaaag tacagacatg ttagctgaag
     6661 ctcttgcagt gatttccaat ctacaggtgt ggactgaaca gcaggatggg attggaaaag
     6721 caaagctaat ggagtattac cgccgcctcg acgcactgta ctgttgtgca aaagagaaaa
     6781 taggtcttct ttcagataac cgggatgctg aactaggctg tgtcccttaa tcatctgagt
     6841 tatagtaact gtctgtttac acaacatatt gaactatggc tcgctacgac cttcccgatg
     6901 aggcatggac catcatcaag cccctgttac ctcctgaacc cgccacacca cgggccggac
     6961 gcccatgggc tgagcaccgt aaaatcatca atggcatgtt ctgggtgtta tgttccagtg
     7021 caccatggcg cgatttaccc gaacgatatg gggcatggaa aactgtttat aaccgcttta
     7081 accgatggtc aaagtcaggc gtgattaata ttattttcaa caggttgctt tcgctacttg
     7141 atgcaaacgg ctttattgac tggtctgcca ccgcgctgga tggcagtaat atccgggcgc
     7201 tgaaatgtgc cgccggtgct caaaaaaaca tcccgatatc gacggagata atgggccggg
     7261 tcgctctcgc ggcggttttg gcaccaaaat ccatctggca acagacggaa gtggcctccc
     7321 gttaaatatc gtgctaagcc ccggacaagc tcatgaaagc cagttcgcgc aacgacttct
     7381 ggacgggatt ggcgttcagc gtcagaacgg cagcatgaaa cgccgtggac atgcggtact
     7441 ggctgataaa gcgta
//
LOCUS       DAJTSA010000156         7374 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000156 DAJTSA010000000
VERSION     DAJTSA010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.156"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            51..1541
                     /locus_tag="OO369_003817"
     CDS             51..1541
                     /locus_tag="OO369_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5983913.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1593..2750
                     /locus_tag="OO369_003818"
     CDS             1593..2750
                     /locus_tag="OO369_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5983914.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2716..2838)
                     /locus_tag="OO369_003819"
     CDS             complement(2716..2838)
                     /locus_tag="OO369_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983915.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3041..4726
                     /locus_tag="OO369_003820"
                     /pseudo
     CDS             3041..4726
                     /locus_tag="OO369_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4764..7124
                     /locus_tag="OO369_003821"
     CDS             4764..7124
                     /locus_tag="OO369_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5983916.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7181..>7374
                     /locus_tag="OO369_003822"
     CDS             7181..>7374
                     /locus_tag="OO369_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983917.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGS"
BASE COUNT         2163 a         1615 c         1567 g         2029 t
ORIGIN      
        1 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
       61 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
      121 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
      181 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
      241 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
      301 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
      361 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
      421 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
      481 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
      541 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
      601 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
      661 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac
      721 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
      781 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
      841 aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc gataatggtg
      901 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
      961 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     1021 attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata
     1081 tcagcattcc aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata
     1141 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     1201 atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg
     1261 attacccgca taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata
     1321 acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg
     1381 atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag
     1441 gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga
     1501 agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg
     1561 atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt
     1621 ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc
     1681 atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt
     1741 ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt
     1801 ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaata
     1861 cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg
     1921 aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca
     1981 gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat
     2041 atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg
     2101 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag
     2161 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
     2221 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg
     2281 ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt
     2341 tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg
     2401 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
     2461 acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac
     2521 ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta
     2581 agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa
     2641 tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag
     2701 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc
     2761 ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
     2821 tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg
     2881 tcagggcatt agcaataccc ttgattatca atagcataac cataaccact gaaaattaaa
     2941 aatttctata tatttcatat tgttaaatga tgcttaaata aatttcatca gtaaatcatt
     3001 gccatagata atcattggat ggcacaatca attttcgcta atgataacca ttcccattac
     3061 gttttgtatg ttgatagcaa agtatctctg tctgctgaag cccttttggc tgcgtaagaa
     3121 taataaaact tcagtgttgt taatcattat tatcctcgca atgatccttg gcgtggttaa
     3181 aattcaggtc tggttaaacg actggaataa tgattttttc aatgcgctaa gccagaaaga
     3241 aaccgataag ctttggcagc tcgttctttg gttccctgcc ctgctgggga tctttgtatt
     3301 aatatccgca aacaaaacat ggttaattaa actactgact attcgctggc gcgaatggtt
     3361 aacagattac taccttaatc gctggtttgc agataaaaac tactacttca cgcagatcta
     3421 cggcgagcat aaaaatacag ataaccctga ccaacgtatc gctgaagaca ttctcttact
     3481 gatcaacaaa acgctcagtc tttcatttgg cttcatccag tcacttagca tgctgatcac
     3541 ctttaccgtt attctttggc aaagcgcggg tacgctctca ttcactgttg gtggaacaga
     3601 atggaatatc caggggtata tggtctatac cgtcgtgctc attgtgatcg gtggaacttt
     3661 atttacccat aaggtgggta aacgtattcg tccgcttaat gtggaaaaac aacgtagcga
     3721 agcgacattt cgaactaatc ttgtgcagca taataagcag gcagaactga ttgcgctaag
     3781 caatgctgag tctctacaac gccaggagtt gcgcgacaat tttcatacca ttaaagagaa
     3841 ctggcatcgt ttaatgaatc gtcagcggtg gcttgattac tggcaaaata tttattcgcg
     3901 ctcgcttagc gttcttccct actttctgtt attgccgcag tttattagcg ggcagatcaa
     3961 tctgggcgga ctgatgaaat cgcgccaggc atttatgcta gtatcgaaca atttaagctg
     4021 gtttatttat aaatatgacg aacttgctga actggctgcg gttatcgatc gcttgtatga
     4081 gttccatcaa ctcactgaat agcgccctac gaataagcct aaaaattgcc aacatgcggt
     4141 acaagtggct aatgcgagta ttcgtacgcc tgataataag atcatattag agaacttgaa
     4201 ctttcatgtt tcgccaggca aatggctatt actgaaaggc tactctggtg cgggaaaaac
     4261 cacactgctt aaaacattat cccactgctg gccgtggttt aaaggtgata tttcttctcc
     4321 tgctgacagt tggtatgtgt cacaaacacc gttaatcaaa agcggcttac tgaaagagat
     4381 tatttgtaaa gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag tactgcatca
     4441 ggttggtctg ggaaaattgg ctgcgcgtat tcatgaccac gatcgctggg gagatattct
     4501 ttccagcggc gaaaaacaac gtatcgccct ggcacgatta attttacgac gtccgaaatg
     4561 gatatttctt gacgaaacta cctctcatct tgaggaacaa gaggctatac gcttactgcg
     4621 tttagtgcgt gaaaaactac ccacaagcgg cgtcattatg gttacacatc aacccggtgt
     4681 ctggaacctg gctgatgata tttgtgacat tagcacggtt ttataacaac aataaaagcc
     4741 ctgctgtacg gagatataaa taaatgaagc gagttcttat tcctggcgtc attttatgtg
     4801 gcgctgatgt ggcgcaggcc gtcgatgaca aaaacatgta catgtatttt tttgaagaga
     4861 tgacggtcta tgctcctgtc cctgtacccg taaacggcaa cacgcattac accagtgaaa
     4921 gcatcgagcg tttaccgacc gggaatggca atatcagcga tctgctgaga accaaccctg
     4981 cggtacgcat ggattcaacg caaagtacct cgttgaacca gggagatatt cgccctgaga
     5041 aaatctctat tcacggtgcg tcgccctacc agaatgccta tttgattgac ggtattagtg
     5101 caactaataa cctgaaccca gcgaatgagt ccgatgccag tagtgcaacc aatattagcg
     5161 ggatgtcaca ggggtattat cttgatgtca gcttactgga caatgtgacg ctttatgaca
     5221 gttttgtgcc ggttgaattt ggtcgcttca atggcggggt aattgatgca aagatcaaac
     5281 gcttcaacgc tgatgatagc aaggtgaaat tgggttatcg cactacgcgt ttggactggt
     5341 taacatcgca tatcgatgag aataacaaga gcgcatttaa tcaaggttct tcaggaagta
     5401 cctatttctc tccagatttt aaaaagaact tttatacctt gtcgtttaat caggagctgg
     5461 ctgataactt tggcgttacc gccggtttat cgcgccgcca gtctgatatc acccgcgcgg
     5521 attatgtttc gaatgacggc attgtcgccg gtcgggcaca gtataaaaac gttatcgata
     5581 ctgcattgag caaatttacc tggtttgcca gcgaccgctt tacccacgat ttaaccttaa
     5641 aatataccgg ctccagccgt gattataata ccagcacctt cccgcagtct gatcgcgaaa
     5701 tgggtaataa atcctatggt ctggcatggg atatggatac gcaactcgca tgggccaaac
     5761 tacgtaccac cgttggttgg gatcatatta gtgattatac ccgtcacgat catgacatct
     5821 ggtacaccga actttcatgt acatatggtg atattacagg gcgttgtacc cgtggcggat
     5881 taggacacat ttcccaggct gtagataatt acaccttcaa aacacgcctg gactggcaaa
     5941 aattcgccgt gggtgatgtt tcgcatcaac cctacttcgg cgcggaatac atctattccg
     6001 atgcatggac tgaacgccat aaccagtctg aatcctatgt gattaatgct gcaggaaaga
     6061 aaactaacca taccatttac cataaaggta aaggcagcct gggaattgac aactacacgc
     6121 tgtatatggc ggatcacatt agctggcgga atgtgtcgtt aatgcccggt gtgcgttatg
     6181 actatgacaa ctatctgtca aaccacaata tctccccgcg ctttatgacg gaatgggata
     6241 tttttgctga tcaaacctca atgattaccg ccggttataa ccgttactat ggcgggaata
     6301 ttcttgatat gggattacgt gatatccgca atagctggac ggaatcggta tcaggtaata
     6361 aaaccctgac gcgttatcag aatttgaaaa cgccttataa cgatgaactg gcaatgggat
     6421 tgcagcaaaa aatcgataag aacgttattg cacgcgcaag tgaagcgcat gatcaaatca
     6481 gcaaaagcag tcgtaccgac agcgcgacta aaaccaccat tactgaatat aacaacgatg
     6541 gcaaaaccaa aacgcattcg tttaacctca gttttgaact ggccgaaccc ctgcatatcc
     6601 gccaggtaga tattaacccg caaattgtct ttagctatat caagagcaag ggcaacttgt
     6661 cgttaaacaa tggttatgag gagagcaata ccggtgataa ccaggtggtt tataacggta
     6721 atctggtctc ttacgatagc gttccagtgg cagattttaa taacccatta aagatctcct
     6781 taaacatgga tttcacgcat caaccgagcg ggttagtgtg ggcgaatacg ctggcctggc
     6841 aagaagcgcg taaagctcgc attatccttg gtaagacaaa tgcgcaatac atcagcgaat
     6901 attcagatta caagcagtat gttgacgaaa aactggatag cagcctgacc tgggacaccc
     6961 gcttgtcctg gacgccacaa tttctgaaac aacaaaacct gacgatcagt gccgatattc
     7021 tcaatgtact ggatagcaaa accgctgttg atacaacgaa caccggtgtg gcgacctacg
     7081 ccagtggccg tactttctgg cttgatgtca gcatgaaatt ttaatagttc ggattttact
     7141 taaccgggta aattcgcccg gttttcgcat ggagataatt atgagaaacc tctgtttctt
     7201 actgacgtta gtggcaactc tgttgctccc cgggcggctg attgccgccg ccttaccgca
     7261 ggatgaaaag ttaattaccg ggcaactgga caatgacttg cgatatatga tttatccgca
     7321 tgctcatcca aaggatcagg taaatttatg gctgcaaatt cataccggtt catt
//
LOCUS       DAJTSA010000157         7188 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000157 DAJTSA010000000
VERSION     DAJTSA010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.157"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            28..879
                     /gene="hchA"
                     /locus_tag="OO369_003823"
     CDS             28..879
                     /gene="hchA"
                     /locus_tag="OO369_003823"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR5983918.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(987..2345)
                     /gene="hprS"
                     /locus_tag="OO369_003824"
     CDS             complement(987..2345)
                     /gene="hprS"
                     /locus_tag="OO369_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR5983919.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="OO369_003825"
     CDS             complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="OO369_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR5983920.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3149..3562
                     /gene="hiuH"
                     /locus_tag="OO369_003826"
     CDS             3149..3562
                     /gene="hiuH"
                     /locus_tag="OO369_003826"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR5983921.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3671..4675
                     /gene="msrP"
                     /locus_tag="OO369_003827"
                     /pseudo
     CDS             3671..4675
                     /gene="msrP"
                     /locus_tag="OO369_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4676..5311
                     /gene="msrQ"
                     /locus_tag="OO369_003828"
     CDS             4676..5311
                     /gene="msrQ"
                     /locus_tag="OO369_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR5983922.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            5569..6219
                     /gene="zinT"
                     /locus_tag="OO369_003829"
     CDS             5569..6219
                     /gene="zinT"
                     /locus_tag="OO369_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR5983923.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         2032 a         1629 c         1507 g         2020 t
ORIGIN      
        1 taactaagca acaataagga atacactatg actgttcaaa caagtaaaaa cccgcaggtc
       61 gatattgctg aagataatgc gttcttccct tcagaatatt cgcttagcca atatacgagt
      121 cccgtctctg atcttgatgg cgtggactat ccaaaaccgt atcgcggtaa acataaaatt
      181 ctggtgatcg ccgcggacga acgttatttg ccgaccgata acggaaaact gttctcgacc
      241 ggtaaccatc cgattgaaac gttgctgccg ttgtatcatc tccatgctgc aggtttcgaa
      301 ttcgaagtgg cgaccatttc cggtctgatg accaagtttg aatactgggc tatgccgcac
      361 aaagatgaaa aagtaatgcc attctttgaa cagcataaat cgttattccg taatccgaag
      421 aaactcgcgg atgttgtcgc cagcctcaac gctgatagcg aatatgcagc aatctttgtt
      481 cctggtgggc atggcgcact tattggttta ccagaaagcc aggacgtggc tgccgcttta
      541 cagtgggcaa tcaaaaatga ccgttttgtt atctcccttt gccacggtcc ggcggctttt
      601 ctggcacttc gccacagcga taacccactg aatggttatt ctatttgcgc attcccagac
      661 gccgcagaca aacaaacgcc agatattggc tatatgccgg gtcatctcac ctggtacttc
      721 ggcgaagagt tgaagaaaat gggcatgaac atcattaatg acgacatcac cgggcgagta
      781 cataaggacc gtaaacgtct caccggcgac agtccttttg cagcgaatgc gttgggtaaa
      841 ctggcggcgc aggaaatgct ggcagcttac gcgagttaat ccctgttaca ttgtgatgta
      901 agcgcataat caatcgcagc cacgttaata ctcacgacgt ggctgcgttt tttcacggtt
      961 aacttatggc gtactgaagc ccgatgttaa tttctttgcg gtaacgtaat tcggaacacg
     1021 ttatgcttac tgagatagtg atacgtagca cttcctccat gtaattcggc aatcgctttg
     1081 actaaagaaa ggccaagtcc ctgacctacg gaatgacgcg aattatctcc ccgccaaaat
     1141 ctacggaaga gtttttcagg ctcattaatt ttcgttccag gactggcgat atcaatatta
     1201 agagagccgt tggcatcaag aaaactggtt atatgaatac gcgatttttc tggtgaatat
     1261 cgaatggcgt taacaataag attcgataac attcgttgca gtaaaatttt atccgcaaag
     1321 atttgttgat tgcacttgac cttaaagcaa atctctttct cgtctgaaag atattcaaga
     1381 tagtccaaaa gattttcaac ttccttattg agagaaagcg aatccagttt caccaaaaca
     1441 ttgtttttgt ctgccctggc aagaaacagt atattctctg ttaaccgcga aatattttcc
     1501 agctcttcaa tgtttccggc aattgttttt tgatattcag cgatacttct gatttgactg
     1561 agcgtaacct gattctgacc cagtaatgca ttaattggcg ttctaagttc atgagcgaga
     1621 tcgtcagcaa attgactaag gcgctcaaaa tctttgacta aagcatgatg cattttattc
     1681 aacgcctgcc caagaggttt tagttctctc ggtaatgcgt taacctcaac cggcactcgg
     1741 ctatcgttat aattcagcgc ttccgttacg ccactcaact ttttgatctc tcgcaacccc
     1801 gtcctgatta acaacggact taatactgag cacagtataa tggcgacaat gcaaattata
     1861 atgctattaa ttttatactg ttcaagcata ttatgtctgg ctgaagccaa tttagccaca
     1921 gtaaccgtta atgatggcga aacttcatca atgttaattc gtaaagcatc tatctctgta
     1981 tcattaataa tgcttctgta aatgcctgct gcgctgattg tctcactagc aggtatgtta
     2041 tttaacatgt catcactgac atttgtccgg ttaacaattt tattgatgcc atcactatga
     2101 atgatcaaaa tatcctgact aacatccatc atccggttaa agtacacagg taacgtatct
     2161 ggatttaccc catcaattaa caactgcttg atctgcgctg tccggttaat gagtgttgta
     2221 tcatcgcgcc atttcaactc acttgccagg ccattataga gagtccatac aattccagcg
     2281 ccagcaacag actgcagaaa tataaaaagc aaggttaaac ggacggttat agataatctt
     2341 ttcattattt ttttaccgct acgaatgaat agcccatccc ccggattgtg gcgattagct
     2401 tttcaggaaa aggatcatca acttttgcgc ggagcctgcg aatggcgaca tccaccgtat
     2461 tggtatcact atcaaagttg attccccaaa tttcactcgc aataaccgtt ctgggtataa
     2521 tctcgccagc tctggaggcc agtagccaaa gtaactgaaa ctccttgcgc gtcagtgtaa
     2581 tactgatatt gtccctgctg acactttgac tgacagagtc catttttaag ccactgattt
     2641 ctaatgttga attcagagcg tgatgttgcc ttaattgtgc ccgaaccctt gctagcaact
     2701 cagaaaatga aaaaggtttt accagataat catttgcccc actgtccaac cctctgactc
     2761 tgtcatcgac agaatccctt gcagtaaggc aaataacagg ggtttgcttt gccgttctta
     2821 acgtttgtaa gatctgccag ccatccatac ccggaagcat aatatccaga atgatcaatg
     2881 cataatcatc cttcagcgca agataaagcc catctctgcc atcagaaacg gcatcaatga
     2941 cataacccgc ttcggaaagc ccctgcgtta cccattcctg ggtcctttga ttatcttcaa
     3001 taagtagaat cttcatgaaa ataatatgcc ataaatcata tgttatagaa acaacctggt
     3061 tcattacaaa attgtaatgc tgctgtaagg ttaccctgga cgttttttcg ctatcctcaa
     3121 aactcattca catgacaagg atataaacat gttaaagcgt tatttagtac tctcggtagc
     3181 aacggcagcc ttttcattac cttctttggt ttatgccgca caacaaaaca ttcttagcgt
     3241 gcacattttg aaccagcaaa caggaaaacc cgctgccgac gtgacagtca ctcttgaaaa
     3301 gaaagcggat aacggctggt tacaacttaa taccgccaaa acagataagg atggacgaat
     3361 taaggcactg tggcccgagc aaactgcgac tactggcgat taccgtgtcg tatttaaaac
     3421 cggggactat ttcaagaaac aaaatcttga aagtttcttc cctgagattc ccgttgaatt
     3481 tcatattaat aaagtgaacg agcattatca tgtgcctttg ttactcagcc aatatgggta
     3541 ttcaacctat cgtggcagtt aatgcgtaaa tatgtaaaag ctgatctctg ctactgttag
     3601 ggaggtgact ccctgacaga ttaacagtaa acagctcttg ttggttaacg acaaaaaagt
     3661 gtgatggctt atgaaaaaga agcaattttt aaaagaatca gatgttacgg ccgagtcggt
     3721 attctttatg aagcgtcggc aggtgttaaa agcactgggc atcagcgcag ctgcattttc
     3781 tttgcctcac gctgcgcatg ccgatctgct tagctggttt aaagggaacg atcgtccgcc
     3841 cgcccccgcc ggaaaaccgc tggagttcag caagcctgcc gcctagcaaa ataacctgcc
     3901 actgacgcca gcagataaag tctccggtta taacaacttc tatgaattcg ggctggataa
     3961 agacgatccc gcggctaatg ctggtagcct gaaaaccgat ccatggacac tgaaaatcag
     4021 cggcgaagtg gcaaaaccat tgaccctcga tcatgatgat ttaacccgtc gcttcccgct
     4081 ggaagagcgt atttatcgta tgcgctgcgt ggaagcgtgg tcgatggtgg tgccgtggat
     4141 tggttttccg ctacacaaat tgctggcgct tgccgaaccc accagcaatg cgaagtatgt
     4201 cgctttcgaa acgatttatg cgccggaaca gatgcctggc cagcaggacc gctttatcgg
     4261 cggcgggctg aaatatcctt atgtcgaagg attgcgtctc gacgaagcaa tgcatccgct
     4321 cacactgatg accgtaggtg tttatggcaa ggcgttacca ccgcaaaatg gcgcgccggt
     4381 gcgactgatt gtgccgtgga aatatggctt taaagggatt aaatcgatcg tcagtattaa
     4441 gctgacccgc gagcgtccgc caaccacctg gaatctggca gcgcctgacg aatacggttt
     4501 ttacgccaac gttaatccgc atgttgatca cccgcgctgg tcacaggcta ccgaacgatt
     4561 tattggttca ggcggcattc tcgatgttca gcgccagcca acactactgt ttaatggtta
     4621 cgccgaccag gtggcatcgc tgtatcgtgg cctggatttg cgggagaatt tctaaatgcg
     4681 tctgacagca aaacaagtga tatggctgaa agtttgcctg catcttgccg gattgttgcc
     4741 gtttctctgg ctggtctggg cgatcaatca cggtggactg ggtgccgatc cggtgaaaga
     4801 tattcagcat tttaccggtc gcactgcact gaaatttttg cttgcggcct tgttaatcac
     4861 acctctggca cgctacgcaa aacagccgtt attgatacgc actcgccgcc tgttaggctt
     4921 atggtgcttt gcctgggcga cactgcattt aaccagttac gcattgctgg agttgggcgt
     4981 gaataatctg gcgttattag gtaaggagtt aattacccgg ccttatttaa cgttaggaat
     5041 tatcagctgg gttattttgc ttgctttagc gttcacttca acccagtcga tgcagcgaaa
     5101 actcggcaaa cactggcaac agttgcataa cttcgtctat cttgtcgcga tcctggcccc
     5161 aatacattat ctgtggtcag tgaagattat ttcaccgcag ccactcatct acgccgggct
     5221 ggctgtactg cttttagcct tacggtataa gaagttgctt tcccttttca accggttacg
     5281 caagcaggcc cacaacaaat tatctctata acttcatatt gccgacaaac ttcgcgttat
     5341 cccggccgga tgcggcgcga gcgccctatc cggcccacac agatcgcatc aagttatgtt
     5401 tcgtttgtca tcaacctttc atcaccagaa taatttattt tatttcaata agatagttca
     5461 atacacctga gaataccatg ctctcatttc ctaatgagtt gttgcatttt ttcatatgtt
     5521 acaatataac attacacata atgtacatta actctggagg aaattgtttt ggcgattcgt
     5581 cttcataaac tggctgttgc tttaggtgtc tttattgtta gcgctcctgc cttttcgcat
     5641 ggtcatcatt ctcatggcaa acccttaaca gaggtcgaac aaaaagctgc taatggtgtt
     5701 tttgatgata ccaatgtaca aaaccgaacg ctcagtgact gggatggagt ctggcaatcc
     5761 gtttaccctt tactgcaaag tggcaaactt gatcccgtct ttcagaagaa agcggatgca
     5821 gataaaacca aaacatttgc tgaaattaaa gattattatc gcaaaggtta tccgacagat
     5881 atcgaaatga ttggcattga ggacggcatt gtcgaattcc atagaaataa tgaaacaaca
     5941 tcctgtaaat atgattacga tggatacaaa atactcacct ataaatcagg caagaaaggc
     6001 gttcgttact tattcgaatg taaagatcct gaaagcaaag cccctaaata tatacaattt
     6061 agcgatcata taattgcacc acgaaaatca tctcattttc acatttttat gggtaatgat
     6121 tcccagcaat ctttattgaa tgaaatggaa aactggccaa cgtattatcc ctatcagttg
     6181 agtagcgaag aagtggtcga ggagatgatg tctcattgat ttttttgcgc tgacttcagt
     6241 tatttaacag aacagtgcct ggtagtaaca acaataattt accaagacaa ataagtaaga
     6301 caaactcaga cacgctcata cttgagtcgt gtctttttta ttatgtctgt agaaaaaaac
     6361 tgagaaaaag gctaacaata aatacacaac tcagccatac agactggcag cgcaaatgtt
     6421 tccaggagca agtcatctgt catggtaaag ctaaccagaa atataggtta attttggtgt
     6481 ttaaaccttt tttacttgtt agtatgatac aaaggctttc aaaaaagctg ggaaattcaa
     6541 caactcttgg aaattatgga ctttgtcccg cggctttaat ctgacggccg cgttcctttt
     6601 ttggctcaat tattgacccc ttctctacgc aacttcagtt cccgccagca actttgcggc
     6661 agtttcatag gatcaatgtc taaaagaata atggtgaccg ataagaaaac gactgaataa
     6721 ctgtagattt tcgctcgaaa ccttcctgtc agctccatag cgaatcaagc gctgaatgtc
     6781 acagtatcga acagaaaaca gtgacgatct aacccttcaa gaatattcta cgattgttct
     6841 gtttaggaaa agcaaggcgg gaagtcggga gataagtcat tgataaaatg gcggagagag
     6901 ggggatttga acccccggta gagttgcccc tactccggtt ttcgagaccg atgatacaga
     6961 gtcgaaaaat caaagagtta aaaatttttc ttggaaaaaa tgtcttaaaa tcgtacacga
     7021 aaaatcaatg aattacataa cattaaagaa gaatattcca agccttcttg agtccaccac
     7081 ctgttttcag gtgtcattca gacgtgattt gccccagatt gggaaagcac gcttttttct
     7141 cagcttattc cgttttgcct gtgtgttgaa taattccact gcagttat
//
LOCUS       DAJTSA010000158         7183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000158 DAJTSA010000000
VERSION     DAJTSA010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.158"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(296..1198)
                     /locus_tag="OO369_003830"
     CDS             complement(296..1198)
                     /locus_tag="OO369_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCR5983924.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTLVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="OO369_003831"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="OO369_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCR5983925.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="OO369_003832"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="OO369_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCR5983926.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="OO369_003833"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="OO369_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCR5983927.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="OO369_003834"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="OO369_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCR5983928.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="OO369_003835"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="OO369_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..>7183
                     /locus_tag="OO369_003836"
     CDS             7090..>7183
                     /locus_tag="OO369_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HCR5983929.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGA"
BASE COUNT         1676 a         1812 c         1876 g         1819 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cagagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcagcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gtattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgc
//
LOCUS       DAJTSA010000159         7157 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000159 DAJTSA010000000
VERSION     DAJTSA010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7157
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.159"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            17..1075
                     /locus_tag="OO369_003837"
     CDS             17..1075
                     /locus_tag="OO369_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5983930.1"
                     /translation="MSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCD
                     TIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYF
                     AMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKG
                     IVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHD
                     LPGGIRVSLSIDILYFITSSRKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMT
                     GLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLHDAY
                     KASDERLYVNKQNKNSRS"
     gene            1118..1621
                     /gene="yeaK"
                     /locus_tag="OO369_003838"
     CDS             1118..1621
                     /gene="yeaK"
                     /locus_tag="OO369_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR5983931.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1622..1726)
                     /locus_tag="OO369_003839"
     CDS             complement(1622..1726)
                     /locus_tag="OO369_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983932.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1896..2342
                     /locus_tag="OO369_003840"
     CDS             1896..2342
                     /locus_tag="OO369_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR5983933.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2299..3117)
                     /gene="nimR"
                     /locus_tag="OO369_003841"
                     /pseudo
     CDS             complement(2299..3117)
                     /gene="nimR"
                     /locus_tag="OO369_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            3214..4394
                     /gene="nimT"
                     /locus_tag="OO369_003842"
                     /pseudo
     CDS             3214..4394
                     /gene="nimT"
                     /locus_tag="OO369_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4449..4796
                     /locus_tag="OO369_003843"
     CDS             4449..4796
                     /locus_tag="OO369_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR5983934.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4818..5072)
                     /gene="yoaF"
                     /locus_tag="OO369_003844"
     CDS             complement(4818..5072)
                     /gene="yoaF"
                     /locus_tag="OO369_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCR5983935.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5255..6280
                     /gene="dgcP"
                     /locus_tag="OO369_003845"
     CDS             5255..6280
                     /gene="dgcP"
                     /locus_tag="OO369_003845"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCR5983936.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVLWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6313..6411
                     /locus_tag="OO369_003846"
     CDS             6313..6411
                     /locus_tag="OO369_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCR5983937.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGCYAAPGA"
     gene            6414..6488
                     /gene="yoaJ"
                     /locus_tag="OO369_003847"
     CDS             6414..6488
                     /gene="yoaJ"
                     /locus_tag="OO369_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCR5983938.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6649..6840)
                     /locus_tag="OO369_003848"
                     /pseudo
     CDS             complement(6649..6840)
                     /locus_tag="OO369_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004995861.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7020..>7157)
                     /locus_tag="OO369_003849"
     CDS             complement(7020..>7157)
                     /locus_tag="OO369_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5983939.1"
                     /translation="AGLKPDTMRNVFRCHIPRYEAIIARYLDMEPAQIWPSRYMEKTS
                     C"
BASE COUNT         1729 a         1741 c         1742 g         1945 t
ORIGIN      
        1 tgctgagcag tcggaagtgt cgccagaaca gcggcgcgcc atctgcgagg cttttgagtc
       61 tgccaataat acccatggct taaacctgac tgcccataaa tacccgggct tacgcggcac
      121 actacaaacc gcatccactg actgcgacac aattgtggaa gctgcagcac tattacccgc
      181 ttttgatcag gcagtggaag gcaaccgcca ccaggatgat tacggttcag gtcttgggat
      241 ggccgaagag aaatttcact attatctcga tctcaatgac cgctatgtct atttttatga
      301 gccggttaat gttgaatact ttgcgatgaa taactggtcc ttcctgcagt caggaagtat
      361 tggcatcgat cgcaaagata ttgaaaaggt atttaccggg cgtaccgtat tgtcgagcat
      421 ttaccaggat cagcgtacta aacagaacgt gatgagtttg ctgacgccgg tatatgtcgc
      481 agggcagcta aaagggattg tgctgctgga tattaacaaa aacaatttgc ggaatatctt
      541 ttacactcat gaccgccctc tcctctggcg ttttctcaat gtcacgctaa ccgataccga
      601 ttcggggcgc gacattatca tcaaccagag cgaagataat ctgttccagt atgtcagtta
      661 cgtccatgac ttacctggcg gcattcgtgt ctcgttatcc attgatattc tttactttat
      721 cacgtcttcg aggaaaagcg ttctgttctg gattttgacg gcgttaattt tgctgaatat
      781 ggtccggatg cacttccgtt tataccaaaa tgtgtcgcga gaaaatatta gtgatgcgat
      841 gactggactg tataatcgca aaattttaac ccctgaactg gagcagcggt tgcagaaact
      901 ggtgcaatcc ggttcttcgg tgatgtttat tgcaattgac atggacaagt taaagcaaat
      961 aaatgacacc ctcggtcatc aggaggggga tttagcgatt acgttacatg atgcgtataa
     1021 agcttccgat gagcgtttat atgtcaataa gcagaacaaa aacagccgtt catgataacc
     1081 ttctgtggtt gtttgcttgt aatctcagga gcgtgaaatg actgaaatgg ctaaaggaag
     1141 cgtgacgcat cagcagttaa tcgctttatt atcacaagaa ggtgctaact tccgcgtcgt
     1201 tacccatgaa gcggtaggta aatgtgaagc ggtgtcagaa attcgtggta ccgctctagg
     1261 ccagggtgct aaggcactgg tatgtaaagt caaaggcaat ggcgtaaatc aacatgttct
     1321 ggcgatcctc gcagccgatc aacaggccga tctgagccaa ctcgccagcc atataggcgg
     1381 attacgcgct tcgttggcca gcccggcgga agttgatgaa ctcacaggct gtgtcttcgg
     1441 cgcgatcccc cctttcagct tccatccaaa actcaaactg gttgccgacc ctctactctt
     1501 cgaacgattt gatgaaatcg ccttcaatgc agggatgctg gataaatccg ttattttgaa
     1561 aaccgcggat tatttgcgca ttgcgcaacc agaactggtc aatttccgcc gcactgcgta
     1621 gctagcctat ccgttcaata agcagaacgg acaaaaccag cacaaccgca atagcaaaaa
     1681 acgatgatgt gataatcagt gtctcgacga acatttgatc gttcatagca tgcccccggg
     1741 agatagctgt tttcacgtta ttgttagcgt gcacaaatgg cagtttgatg acagttcgcc
     1801 tgatttttta tgcaaaaaac gtaaagattt tctacttcct tcctgcagca agcataaagt
     1861 aagcaggctt attatttttt ggcaaggaaa ccacgatgtt tgatgtcact ttgctgatcc
     1921 tgctcgggtt agctgcgctg ggctttatca gtcataacac cactgtcgcc gtttcaattc
     1981 tggtgttgat cattgtccgc gtcacaccgt taagcacctt tttcccgtgg attgaaaaac
     2041 aggggctaag tatcgggata atcattctga cgattggcgt tatggccccc atcgccagcg
     2101 ggacgctacc gccctcaacc ttgattcact cgtttctgaa ctggaaatcg ctggtggcaa
     2161 ttgcagtagg cgttattgtc tcctggctgg gtgggcgcgg cgtgacgtta atgggcagcc
     2221 agccgcaact ggttgctggg ctgctggtgg gcactgtttt aggcgtagcg ctgtttcgcg
     2281 gcgtaccggt tggcccgctt attgccgcgg gtctggtttc gctgattgtg gggaaacagt
     2341 agttaatccc gcaatatatc ggcctggcgt ttgccccaag cctttcttaa acatagtaat
     2401 aaaggccgtt gttgaatcat accccagcgt atgagcaact ttctgtacag tatcgccttt
     2461 cactaatccc tgaagcgcca taatcagctg taactgctga cgccactggc gaaaactcaa
     2521 ccccgtctct ttcactatca ggcgggcaag attgcgttca ctcatcgcaa aaaagccagc
     2581 ccattgcccc aatacccccc actcgacggg ccctttcgcc atcatctcca ccatcgtgcg
     2641 gattttagga tgagaagaaa cgggcaggtg cagctgttgt tgtggctgct gagggagttc
     2701 atcaaacagg acctgaatta atctgcgggt catcggctca gcacgctgag tcgtcgtcct
     2761 gtttgccagg gttaaaatca attcccggca taagggcgat attttcaatg tgcaacatgg
     2821 tcggcattgt cactgcgctg ggttcgataa acagaaaaca gagttcagcg ttagctgtca
     2881 cttgattgct atgcttcacc ccgcccggta tccaaaccgc atattgtggc ggcaccatcc
     2941 acaaagcatt ttccaccgta caggtaattg caccatgtaa tgccagtatc agctgtcctt
     3001 tgcggtgctg atgtacagga ctggttaact catccgttcc ggcgtgaata caaaatgcga
     3061 ccgcggcttc atggtgtcgg tgggttcata gccattgaga ttcaacctgt gcatcatttt
     3121 gtccgaactt agcgataatt tgtcatttta gcttgattca acataacaat aaaaacggta
     3181 aggtacagcc tcgtttgtaa caatgagaag catatgacct gttcaacttc attaagcggc
     3241 aaaaacagga ttgtccttat cgctggcatt ctgatgattg ccacaacatt acgcgtcacc
     3301 tttaccggcg cagcgccgtt actggatacg attcgttccg cttactcgct gacgacagcg
     3361 caaaccggct tattaaccac cctgccatta ttggcctttg cgctaatctc acctctggct
     3421 gccccggtgg cgcgacgttt tggtatggaa cgtagcctgt ttgccgcgtt acttttgatc
     3481 tgtacaggta tcgcaattcg ttctctccct tcgccttact tattatttgg cggtacagcg
     3541 gtcattggcg gtgggattgc attaggcaat gtcttactgc caggattaat taaacgcgat
     3601 ttccctcatt ccgtcgccag acttaccggc gcatattccc tgacaatggg agctgcagcg
     3661 gcactgggat cggctatggt cgtgccgctg gctttgaacg gttttggctg gcaaggcgcg
     3721 ttgttcatgc tgatgtgttt tcctctgctg gctctttttt tatggctgcc acagtggcga
     3781 agtcagcaac atgcaaattt gagtacctca cgcgccttac atactcgggg tatctggcgt
     3841 tcgccacttg cctggcaggt aacattgttt cttgggatca actcactggt ctattacgtg
     3901 attattggct ggcttccggc gatcctcatc agtcacggct atagcgaagc acaggcgggt
     3961 tcactgcatg gtttgctgca actagccaca gcagctcccg gtttgctgat cgcacttttc
     4021 ttacatcatg tgaaagacca gcgtggtatt gcagcgttcg ttgccttgat gtgcgcagtg
     4081 ggcgcggttg ggctctgctt tatgccagcg cacgcgatca cctggactct gcttttcggt
     4141 tttggttccg gcgcaacaat gatactgggg ttgacgttca ttggtcttcg gcaagttctg
     4201 cgcatcaggc ggcggcactc tcggggatgg cacaatccgt cgggtatttg ctggcagcct
     4261 gtgggccgcc gctaatgggt aaaatacacg atgctaacgg taactggtcc gtaccactga
     4321 tgggtgttgc aatactttca ctactgatgg cgattttcgg actttgcgcc gggagagaca
     4381 aagaaattcg ctaatatccg gtgctatagt gacgtgacaa atcttgcgtg aaagggagaa
     4441 caaacacgat gaatattcag tgcaaacgcg tttatgatcc ggctgaacag agcgatggtt
     4501 atcgcgtact ggtcgaccgc ctctggccgc gcggtatcaa aaaaaccgat ttagcccttg
     4561 atgagtggga taaagaaatc acgccgtcaa cggaactgcg caaagccttt cacggcgaag
     4621 tcgtcgattt tgcaaccttt cgcgagcaat atcgcgcaga actggcgcaa cacgagcaag
     4681 aaggaaagcg gctggcggac atcgccaaaa aacagccgct gaccctgctc tacgcagcaa
     4741 aaaacaccac gcagaaccat gcgctggtgc tggccgactg gctacgtagc ttgtgatttt
     4801 agtacggcat ccggcggtta tttttcacca gccggatggt cccgtcgcca caatgcccat
     4861 tcatcaatcg tttcaccgcc cggtaatttg caattgttgc tgaccccttg cgctgtctgc
     4921 actggaatga gcgtcccgcc cttctgctgg caatagaccg acgccggatt tgccatacca
     4981 atctgcggcg gtttaggtgc ttctggctga gaaggggttg aacaaccagc caggaccagc
     5041 aagcaaggca gaacaaaact gataattttc atttattgat ctcacatatt tatccaagct
     5101 tagagtatcg cggtatcgtt ttgttttgca gcactatttt tattacattc actcaaaaca
     5161 tattacgtct tgtttcatct ttgttgatga tgttttatca tgcctgcaaa gattaaataa
     5221 tcagcactta cccgccgtat cctggagttg ttccgtgtca gatcagatta tcgcccgcgt
     5281 ctcgcaatcc cttgccaaag aacagtcact ggaaagtctg gtccgacagc ttctggagat
     5341 gctggaaatg gtcactgata tggaatcaac ctacctgacc aaagtggatg tcgaagcgcg
     5401 cctgcagcat attatgtttg cccgtaacag ccagaaaatg cacatcccgg agaattttac
     5461 cgtcttgtgg gattactcgt tatgcaaacg cgccattgat gaaaactgct ttttcagcga
     5521 tgaagtccct gaccgttggg gcgactgtat tgcggcacgc aatcttggca tcaccacatt
     5581 tctgagcacg ccaattcact taccggatgg atcattctat ggcacgcttt gcgccgccag
     5641 cagtgagaag cgccagtgga gtgaacgcgc ggaacaggtt ttacagttat tcgccggact
     5701 gattgcacaa tatattcaaa aagaggcact ggttgaacag ctgcacgaag ccaatgctgc
     5761 gctgattgcg caatcgtata ccgactcgtt aaccgggcta ccgaatcggc gggcgatttt
     5821 tgaaaatctg acgacgctgt tttccctcgc ccggcatctt aaccataaga taatgatcgc
     5881 gtttatcgat ctggataact tcaaattaat caatgatcgt tttggtcata atagtggcga
     5941 tctgtttctc attcaggttg gcgagcgcct taatacgctc cagcaaaatg gcgaagttat
     6001 tggtcgtctc ggtggtgatg agtttttggt tgtttcacta aacaacgaga atgcggatat
     6061 ttcgtcgctg cgagaacgta ttcaacagca aatacgtgga gaatatcact taggtgatgt
     6121 tgatttgtat tatcccggtg ccagtcttgg catagtagaa gtcgatcctg aaacaaccga
     6181 tgcagacagt gccctgcatg ctgccgatat tgcgatgtat caggagaaaa aacacaaaca
     6241 gaaaacacct tttgtcgcgc atccagcgct acattcctga ggcgtattca catccttttg
     6301 attggtgata acatgcgaat cggtattatt tttccggttg taatcttcat tacagcggtc
     6361 gtatttttag catggttttt tattggctgc tatgctgccc cgggagcata aagatgaaaa
     6421 aaacaacgat tattatgatg ggtgtggcga ttattgtcgt actcggcact gagctgggat
     6481 ggtggtaacg tcacctctaa aaaatagcaa aggctgcctg tgtgcagcct ttgtgcaatt
     6541 taagcgttaa cttttaatct tcctgtagat aaatagcacg acaatcgcac caataacggc
     6601 tgaagcggcg cacgaaaaac gctttcgcgt ttttcgtgcg ccgcttcacc cctggttcag
     6661 catttcgtct gcagtctgaa ccgcaggcaa ccatttttca gcaagtctgc gctgggaatc
     6721 gctggcttta tcccatttgc tccacagtgc ctcacgatcg taatcgtgcg cttccagcgt
     6781 ggggcgggcg atttcaaaat acccctagct tgtttctatt tgctcagatt ttaataacaa
     6841 ctcagcacgt gctgccgctg gtagaactga tatatgaaat tccaaagcct ttgaacccgt
     6901 acgcttgcga cgtagttctg gatgattggc tgcaagcttt tcaagattgc ggcgaatact
     6961 cacctcatgt ttaggaaagc atgacattcc tacgcattct tgcgctgtta cccaatgggt
     7021 taacatgatg ttttctccat ataccgagaa ggccaaatct gggctggttc catatccaaa
     7081 tatctagcaa tgattgcttc gtatcgtggt atatggcagc gaaatacgtt acgcatagtg
     7141 tcaggcttta agcctgc
//
LOCUS       DAJTSA010000160         6795 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000160 DAJTSA010000000
VERSION     DAJTSA010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.160"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            240..2672
                     /locus_tag="OO369_003850"
     CDS             240..2672
                     /locus_tag="OO369_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR5983940.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2739..4208
                     /locus_tag="OO369_003851"
     CDS             2739..4208
                     /locus_tag="OO369_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR5983941.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4208..5869
                     /locus_tag="OO369_003852"
     CDS             4208..5869
                     /locus_tag="OO369_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR5983942.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6090..6431
                     /gene="symE"
                     /locus_tag="OO369_003853"
     CDS             6090..6431
                     /gene="symE"
                     /locus_tag="OO369_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCR5983943.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6478..>6795)
                     /locus_tag="OO369_003854"
     CDS             complement(6478..>6795)
                     /locus_tag="OO369_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983944.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT         2003 a         1612 c         1554 g         1626 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaaactggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta
     3841 ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga
     3901 aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt
     3961 ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca
     4021 gcattgatga cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg
     4081 aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga
     4141 tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg
     4201 ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt
     4261 gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa
     4321 gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc
     4381 gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt
     4441 gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc
     4501 gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc
     4561 tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat
     4621 cgagcaaagc caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt
     4681 aaataatcca gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga
     4741 ttttgttgtc ggcgctgacg gtgtgaagat tttatcacct atattaattt gtgagcgttt
     4801 ctttttttgg cagttacgat catttaaatt agatgtaaga ggatacgcga gacattttaa
     4861 agtattaaat agctgcttat ttgcattacc ccccattgca gaacaagagc gaatagttga
     4921 aaaagttagc tctttgatgt ccctctgcga ccaactagaa cagcaatccc tgaccagtct
     4981 ggacgcacat cagcaactgg ttgaaaccct gttgggaacg cttacagaca gccaaaacac
     5041 cgcggaactg gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc tatttaccac
     5101 cgaagccagc gtggatgcgt taaaacagac cattctgcaa ctggccgtaa tgggcaaact
     5161 tgtgccgcag gatccgaatg acgaacccgc ctctgaactg ctcaaacgta ttgcgcagga
     5221 aaaagctcaa ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc cgccaattag
     5281 cgatgaggaa aaaccgtttg aacttccgga agggtgggaa tggtgtcgta ttggtaatat
     5341 agttaatatt aaatctgaat tagtatcacc aaaagattat ttaaatttat atcaagtagc
     5401 acctgatatt attgaaaagg gcactgggag agtgatatca aagagaacag tgaaagaatc
     5461 aggagtcaaa gggccaaata gtcgctttta taaaggacaa attgtttatt ctaaaatccg
     5521 cccgtcatta tccaaagttt ttttagcaga gtataatggt ctttgcagtg ctgatatgta
     5581 tccattagac tgttacatta atccaaatta cttattgaaa tatatccttt ccattccatt
     5641 tcttatgcaa gtcaaaaaag cagagaacag aataaaaatg ccaaaactta attctgacag
     5701 tttttacaat attattgttg cgattccccc ctataatgaa caacaagcaa tatttgataa
     5761 gataaattct attgaagcag tatgcaatgg tcttatttct tatattggga tttatcataa
     5821 aacccaactg catcttgcag acgcactcac tgacgcagcg ataaactaat tccctcgccc
     5881 tcacaaaacc aataaagcgg ctaaatagcc gctttattca catcaccaaa atttatattt
     5941 cacgctaatt ttcatcttat tttcattaat ttttagctgg actttccctg tatttactga
     6001 tgatgtatcc aggtatttag cacggtgcgg atgtgcgcca acacactcgc accgctaatc
     6061 acaatcacta tttatagaga atagtaatca tgactgacac gcattctatt gcacaaccgt
     6121 tagaagcaga agtctccccg gcaaataatc gtcaattaac cgtgagttat gcgagtcgtt
     6181 acccggatta cagccgtatt cccgccatca ccctgaaagg tcagtggctg gaagccgccg
     6241 gttttgccac cggcacggcg gtagatgtca aagtgatgga aggctgtatt gtcctcaccg
     6301 cccaacccgc cgccgccgaa gagagcgaac tgatgcagtc actacgcaag gtgtgtaagc
     6361 tgtcggcgcg taaacaaagg caggtacagg aatttattgg ggtaattaca ggtaaacaga
     6421 aagtcgcctg atacattgac ccggtagcgc cagtgctacc gggtattatt cgccgcgtta
     6481 taccaacgcg ctctccatct gtttttcaaa tgcctgacga attaatccca taatgtaagg
     6541 aagttgtgcg aggtcgctga aaccaatttc tacatcgcca ttaccccagc gcccaacatt
     6601 tgtcacatct ttggcaatac ctttcggatc gaccagttca tgaaactgca tattaagcga
     6661 caatcgcagt cggctttttt gcggcacaac atcaacaaaa ttcgtttccg ctttaaacgc
     6721 aatatatagc ttcaatactt cctgcgtgat ccctgcgtcc aggcgcataa cttcatcgcg
     6781 aagatgatcg aataa
//
LOCUS       DAJTSA010000161         6765 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000161 DAJTSA010000000
VERSION     DAJTSA010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.161"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            58..1482
                     /gene="sbcB"
                     /locus_tag="OO369_003855"
     CDS             58..1482
                     /gene="sbcB"
                     /locus_tag="OO369_003855"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR5983945.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(1525..1752)
                     /locus_tag="OO369_003856"
     CDS             complement(1525..1752)
                     /locus_tag="OO369_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR5983946.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1766..2824)
                     /gene="yeeE"
                     /locus_tag="OO369_003857"
     CDS             complement(1766..2824)
                     /gene="yeeE"
                     /locus_tag="OO369_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR5983947.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3003..4361)
                     /gene="plaP"
                     /locus_tag="OO369_003858"
     CDS             complement(3003..4361)
                     /gene="plaP"
                     /locus_tag="OO369_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR5983948.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(4351..4413)
                     /gene="yoeI"
                     /locus_tag="OO369_003859"
     CDS             complement(4351..4413)
                     /gene="yoeI"
                     /locus_tag="OO369_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR5983949.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(4574..5557)
                     /locus_tag="OO369_003860"
     CDS             complement(4574..5557)
                     /locus_tag="OO369_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5983950.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(5603..6427)
                     /locus_tag="OO369_003861"
     CDS             complement(5603..6427)
                     /locus_tag="OO369_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5983951.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(6510..6764)
                     /gene="yoeB"
                     /locus_tag="OO369_003862"
     CDS             complement(6510..6764)
                     /gene="yoeB"
                     /locus_tag="OO369_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR5983952.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1825 a         1776 c         1586 g         1578 t
ORIGIN      
        1 ggttgctttt cgagttataa cagacagtta attaactaca gtggacaatg ctagacaatg
       61 acagacacag ataaacaacc taccttcctc tttcacgatt acgaaacctt tggcacgcac
      121 cccgcgttag atcgccctgc acagttcgca gccattcgca ccgatgacga attcaatgtc
      181 atcggcgaac ccgaagtctt ttactgcaag cccgcggatg actatttacc ccggcctgga
      241 gcagtattaa ttaccggtat taccccgcag gaggcacggg cgaaaggaga aaacgaagcc
      301 gcgtttgccg cccgtattca ctcgcttttt accgtaccga agacctgtat tctgggctac
      361 aacaatgtgc gtttcgacga cgaagtcaca cgcaacgttt tttatcgtaa tttctacgat
      421 ccttacgcct ggagctggca gcatgataac tcgcgctggg atttactgga tgttatgcgt
      481 gcctgttatg ccctgcgccc ggaaggaata aactggcctg aaaatgatga cggtctaccg
      541 agctttcgcc ttgagcattt aaccaaagcg aatggtattg aacatagcaa cgcccacgat
      601 gcgatggctg atgtgtacgc cactattgcg atggcgaaac tggtaaaaac gcgtcagcca
      661 cgcctgtttg attatctctt tacccatcgt aataaacaca aactgatggc gttgattgat
      721 gttccgcaga tgaaacccct ggctcacgtt tccggaatgt ttggggcatg gcgtggcaat
      781 accagctggg tggcaccgct ggcgtggcat cctgaaaatc gcaatgccgt aattatggcg
      841 gatttggcag gagacatttc gccattactg gagctggata tcgacacatt gcgcgagcgt
      901 ttatataccg caaaagccga tcttggcgat aacgccgccg ttccggttaa gctggtgcat
      961 atcaataaat gtccggtact ggcccaggcg aatacgctac gcccggaaga tgccgaccga
     1021 ctgggaatta atcgtcagca ttgcctcgat aacctaaaaa ttctgcgtga aaatccgcaa
     1081 gtgcgtgaaa aagtggtagc gatattcgcg gaagccgaac cgtttacacc ttcagataat
     1141 gtggatgcac agctttataa tggctttttc agtgacgccg atcgtgcagc aatgaaaatt
     1201 gtgctggaaa ccgagccgcg taatttaccg gcactggata tcacttttgt tgataaacga
     1261 attgaaaagt tgttgttcaa ttatcgggca cgcaacttcc cggggacgct ggattatgcc
     1321 gagcagcaac gctggctgga gcaccgccac caggtcttca cgccagagtt tttgcagggt
     1381 tatgctgatg aattgcagat gctggtacaa caatatgccg atgacaaaga gaaagtggcg
     1441 ctgttaaaag cactttggca gtacgcggaa gagatcgtct aatttgaagc cgccgccgct
     1501 ggtacggcgg aggacaatgt tgagtcaggc tttttgaacg gtgatgctcc acgctgcatc
     1561 gccaatctgc tgataatcgg tgatggcatg tccttcttct gccgcccact gtgggattgc
     1621 ttcagttgcc tgggtgcaat caaactcaat taccaactca tcgccgctgg ccatttctgc
     1681 cagtgctgct tttgcttcta tgagtggaaa cggacagacc tgcgtcacca catccagctt
     1741 tttaatcgcc atctttacac cttacttaat ttgccgcagc agttgccagt cgcgccttac
     1801 gctgcggtcg aacatacaca agccaggacg cagtccagac tccgagaatc ataaatacaa
     1861 ggccaatcca gccctgccag gtcatcattg cagtcataac cagtccatta ccgatagagc
     1921 aaccacctgc aatactggca ccgaagccca tcagtacacc acctaacccg ctacgtaatg
     1981 ttgtttgtgc atcagctgcg cgaacgcgga actcacggct cgctttggct gcaataaatg
     2041 cctccacgaa gatccctaac accaggaaaa cgccccagtt aatgtatttc acgtcacccg
     2101 cgaccagaaa ttgaagaata ttggccgttg gggaagtgac tcctaaccca aacatgcgtc
     2161 cggttgcttc actcaggggc caggctaaaa gcgcaatcaa accgatgagt acagcagtga
     2221 caaagggatg ccagcgtttt tcaaacagaa tatgagcgat cccggttcgg cgcggcggta
     2281 aggtcgcgac tttgagtttt ggcttcttca actctttcat caccacccag agcgttatca
     2341 ccagcaaaac ggtaaccaac ggccacacag acaaattaaa agtctcagca atagagttat
     2401 gttcagtagt gtagtgctgc aaggtttgat ttaaaccact ggcgtgtgga gaacgcatca
     2461 ccgcactcat caccatataa gtgaaaagcg cgatccaact gccgatcaat ccctcacccg
     2521 cgcgatacca ggtcccggtg gcacatccac ccgccagaac aattcccagc ccgaaaagat
     2581 acccacctat aacagtacca agccacggga acgccccggc ttcgtaagtc agtaggcccg
     2641 cctgaattaa cgcaaaaacc cccacgcttt gtgccgaaat tgcaatcagc aaggcgtaaa
     2701 acatgcgatt atttttcgcg atatacatat cgcgaaaacc accagtcagg caaaaacgcc
     2761 cacgctgcat gacaaatccc agcagagcac cacaaattag cccgcttaat atcattgaaa
     2821 acataaatta attaaccaga tgaatgttaa tggggaaatt tttcatgact ggtggaatgc
     2881 agaccaataa ccaaattctc taaattagaa caaatggtta ttaatgagga tttcgataaa
     2941 aaagaataaa aaaaccggag ccaaatgttc attcgactcc ggtttattat tagaaaagat
     3001 gattatgcta cgtcttcgta ctgcggaacc ggattacgga agctcttggt gacgcaagcc
     3061 aggtaaatca gaccgatagc agcccagatc agacccagaa ccattgagct ttcttccagg
     3121 ttaacccaca gcgcaccaac ggtcagcgca ccacacatcg gcaggaacag atactggaag
     3181 tgatctttca gcgtcttgtt acgcttctca cggatccaga actgcgagat gaccgacagg
     3241 ttaacaaagg tgaacgccac cagcgcacca aagttaatca gcgccgttgc cattaccagg
     3301 tcgaagttga ttgccagcag ggcaatcgcg ccaaccagga tgatgttcat cgccggagta
     3361 cgccatttcg ggtgtacata accgaagaag cttttcggga acacgccgtc acgacccatt
     3421 acgtacatca gacgcgctac gcctgcgtgc gccgccatac cggacgccag tacggtaatg
     3481 gtggagaaga tcagcgcgcc aacctggaac gctttacccg caacgtacag catgatttca
     3541 ggctgtgacg catccggatc tttaaagcga gagatatccg ggaagtacag ctgcaggaag
     3601 taagttgcaa agatgaagat catgccgcca atcagcgcgg tcaggaaaat cgcacgcggg
     3661 atcacgcgct ctgcatcttt ggtttcttcc gacagattgc tgatgccgtc aaagccggta
     3721 aaggagaagc acaggattgt cgccccggta atcatcggga taacatgcgc atcgccagac
     3781 cagaacggac gcgtgctcgc cagcgtacca gcgccttcac cttcaaatac gccataaaca
     3841 accatgccca gaatcaccgc gatcagcact acctgcaaca cgacgattac agtgttgacg
     3901 ttcgctacgg atttcagact acgcaggtta aaggcggtca taaaggccac cagcgccacc
     3961 acaaacatcc acgatggaat ggaaggcacc agagcttcaa aatagatttt cgccagcaga
     4021 atgttgatca tcggcgcgaa cagatagtcg agcagagaag accaacccac cataaagccg
     4081 acagtcgggc taatggattt ctgggcgtaa gtgtatgcag agccagcaga aggatagcgg
     4141 cgaaccagct tcccgtagct cagagccgta aacaggatcg caatcaatgc gaacgcatag
     4201 gctgtcggca catgaccatc cgtgaggcct gaaacgatac caaatgtatc aaacagcgtc
     4261 atcggctgca tataggctag acccatcatt acaaccggaa ctaacgtaag cgttttacgc
     4321 aattccacgc gagaggtgtt tggagtaacg ttatgcgaca tggttattct cctttacggt
     4381 gataaccgtc gcgtaagcaa aaaattgccc catttttttg gattcctcag cgacaacaac
     4441 tgtcgatttt tagtaaatat ctatccggta cgaagcccgg cctcttggta tgaatgattg
     4501 gtttgaagca aaaaataacc gacgctaatt aaacgtcggt ttttaatcat tttttgacag
     4561 cggcgcattg tgcctaaatg ggggggaaat gacaagagaa tgagaggctt gtcataataa
     4621 tttttctttg aatggctgat tttccgtcat cagattttgc ataaacaccg gcgatcgcac
     4681 tatttgctaa aatttcatct cgccaccagc cgctggcaag ccctgcggtc tgttcattcc
     4741 agccaatcca gacaggttca aagagagttt gggcgaccac tttcttctcg attaacgcgc
     4801 cactatcgag aaaacgttgc gccagatacc gtggtaaata gccgcagcca aggccgctaa
     4861 tttgcagctc cagtttggtt ttaaaatcga aaacggtaat ggcttcttgt tcgtcgagaa
     4921 gttgtgaggc cgtagaagcg cctgcctgcg cagtatcccc caccacaatc gcccgatatc
     4981 gtttaatgat acgacggttt aacggttctt cttcctgggc taacggatga tgaggcgcga
     5041 ccgcaaagac ttgttcaaga tcgcccaacc gcgagaaacc aaactcactg gaagatggtg
     5101 gctcatgcat cgcgccaacg ataatatccg ctcgcccctg agtcagggca tcccaggaac
     5161 caccgagtac gccattgata aacttcaggc gcgtcacgct atgatgttga taaaaagctt
     5221 caatgagagg cgcaagcaga gaaaaaggaa acgtgtcgtc cacgccaatc accagctcgt
     5281 tttcccagcc ttcatgcagt ttgatcgcct gcttttccag ttctcgcacg gtatgcagaa
     5341 cttcccgccc tttctctaat aacatttttc cggtgcgggt gaatttagcg cggtgtccgc
     5401 tacgatcaag caattgaata tttaggtcgc tttccagctt gtgaacggtg taactcagag
     5461 cggacggcgt cttgtagagt ttggctgacg ccgccgcaaa actgccctct ttttctatgg
     5521 catcgaggat catcaacaca tccagcagtg gtttcataca cgcccccttg cagtgcatgc
     5581 ggcgtcctgc tggcgtgatc gtttactcca acggcattac cagcggatcg ggatactggt
     5641 attcaaatcc cagttcatta caaatccgac tgccatcaat aatcttacct ttgccgctgt
     5701 ccagactatt tctgaactgc ggaggttgca gccccagtaa acgggccatc tgcggataga
     5761 aaacattacg cgcagggtga gctggcgcac atatattata gatgtgtccg cctttaggtg
     5821 cctgtaacaa cagagtgata gcgccaataa catcttctaa atggactaaa ttaacaccat
     5881 gttcaccatc aggggcggtt tttccggcaa agaagcgtcc cggatgacgt cccggtccga
     5941 ccaggcccgc aagacgcaga atatcgaccg aagtaccggg taaattgtgc agccagtctt
     6001 cgagttcttc taatactcgt ccactgttgg ttaccggatt acgcggggta gtttctttaa
     6061 ccgtgccttg cgcgtcgcca tagacagatg ttgagctggt aaaaataatt cggggaatac
     6121 gatgggccag cgcgctatcc actaactctt gtaccgcttg taaatagaac tcatcgccgg
     6181 ggccgctacg acgtgccgga agcgtaatga ccagcgcatc ggcatccata agggcatcca
     6241 gatcgtcaga atcgcaaact aactcaggct ccatgcgaag cagatagcta tcaatgccac
     6301 tcattcgggc cgcttcgaca ccatcttgtg tggttttact cccggtgact tgccagcctc
     6361 gcgctgaaag tgacatcgcc agcggcatac ctaaccaccc taacccgaca attgcgacct
     6421 ttttcatccg ttatctcctg atacacctta gatctataag gctacgctag cgtatcaaaa
     6481 ctgacaattc attctatgaa tgaatctgtt caataatgat aacgacatgc tgcaatgagc
     6541 agtgaatcat cagtaaccgc gtataccaga cggtgctcct ctgtaatgcg tcgggaccag
     6601 aaacctgaca aattatgttt caggggttct ggtttcccct taccttcaaa tggtgttctg
     6661 cgggtatctt tgataagttc attgatcttt ttaacaattc gcttatctgt ttcctgccag
     6721 tacagataat catcccatga ttcctcagac cagattagtt tcact
//
LOCUS       DAJTSA010000162         6588 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000162 DAJTSA010000000
VERSION     DAJTSA010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6588
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.162"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1126)
                     /gene="hemX"
                     /locus_tag="OO369_003863"
     CDS             complement(<1..1126)
                     /gene="hemX"
                     /locus_tag="OO369_003863"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5983953.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEA"
     gene            complement(1148..1888)
                     /gene="hemD"
                     /locus_tag="OO369_003864"
     CDS             complement(1148..1888)
                     /gene="hemD"
                     /locus_tag="OO369_003864"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR5983954.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(1885..2826)
                     /gene="hemC"
                     /locus_tag="OO369_003865"
     CDS             complement(1885..2826)
                     /gene="hemC"
                     /locus_tag="OO369_003865"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR5983955.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGTPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            3213..5759
                     /gene="cyaA"
                     /locus_tag="OO369_003866"
     CDS             3213..5759
                     /gene="cyaA"
                     /locus_tag="OO369_003866"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR5983956.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(5799..6119)
                     /gene="cyaY"
                     /locus_tag="OO369_003867"
     CDS             complement(5799..6119)
                     /gene="cyaY"
                     /locus_tag="OO369_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR5983957.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(6167..>6588)
                     /locus_tag="OO369_003868"
     CDS             complement(6167..>6588)
                     /locus_tag="OO369_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5983958.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1482 a         1723 c         1717 g         1666 t
ORIGIN      
        1 tagcttccgt tgtccccgct gccggttgtg ccagcaggtt acgcacgcga gtctgcatca
       61 gtttttccag catcgcctgg ctttgcaggg tttccggaag atccatcgag atattttgct
      121 ggcttaactg gtccacctcg tcgaggaacg ctttggtggt ggcatcatca gtatcgtagt
      181 aagcacgtac ccaggtggag acgttctcca gcgcctggcg ataagtctct tcctgatgac
      241 gcggtacagc ttgtgctgcg accagcaggc gagagcgaat attttcgcgc agatagatat
      301 cctgatttgg cgctaacagc ggtacggcgg tgtcatcacg acggcgaatc gtaatgaagt
      361 tgtccataaa gttctgccag cttttttgca gattgatacg ccattcgctg atggaactgg
      421 aaagctcttc accgtctgaa tccatcggcg aaccatcgct gtcattatcg gccagacgca
      481 ggttatctac ctgatttgaa agctgattaa gcttaaggat gatgccgtca taatccacct
      541 gcgatactgc agaaaggctg gcgatatcat cggtaattgc ccgacgaacg gtaatcagac
      601 tcgggtcatt catatccgcc aggctggcgt ctgcactttt cagcaacgct gcagaggtcg
      661 tgacgtcctg atcgctccac agcttccgtc cggcgagttt caccagaaaa tcggcctgag
      721 ccagcagcca ggttttagca tcgctgccgg aaatggtggc gatcttttgt tggacttcat
      781 ccaactgttt tgccagcgtt tcttgctgac gattcgcctg cttaagctgc gcagcttgtt
      841 gcttaataat gccttccagc tcggcttttt ggctctcctg ggctttttgc aatgccgtca
      901 gttggttagc cagggcatcg ctggtggcgg tctgattgac ggcctgttgt ttaccccagc
      961 catacaaacc gacacccgcc gccagagcaa tagcgatagc caccgcgctg agaatcaatg
     1021 cagtattgtt cttactcttt ttttctgttg cgacaggttg tgacgtggtg tccacggcct
     1081 ccctggtctc ttcaaccacg gcggaggttt tttcttgttc cgtcattatg gcttcctgtt
     1141 atgagagtta ttgtaatgcc cgtaaaagcg catcgttgtc agcgttatcg gcgaccttaa
     1201 tgtcttgcca gcccagttcc cgggcgagtt tcgccaaacg ctcactgacg accaatagtc
     1261 gacagtgtag taaccagtgc tcacgatacc attgtgggat cagcgaccag agttgctgca
     1321 acatttcacc gctggtaaca acgaccatcg tcacctcgcg ggattgccag cgcatcgctt
     1381 cttctgcacc atcgtaatgg attgcgcatc gttgataaca ttcacaaaaa gtgacctcag
     1441 caccgcgcac cgtcagggta tccccaatta gctcacgacc gccattgcca cgtaatatca
     1501 gcgcacgttt gcccgcaata ttttgtaatt caggtaattg tagcaagact tcgctgattt
     1561 cccgatcctg tgggtagaga atcttctgtc cgcttacggt atgtagcgcc agtgcggtgg
     1621 tgcgtccaat ggcgaaataa gtaggtagtt ggggccattt acgatcttgc tgatgcagct
     1681 gtgattgggc aaaagcaacc gcgtgttgcg agagggcaaa caacagatcg ctctccccca
     1741 gcgctgccag ttgatcagca agttgcggta attgtcgacc cggagaaaac tcaatcagcg
     1801 gaaaatgcca ggccacctgc cccagtgtgc gcagacggct cactaactct tctccagcgg
     1861 gagacgggcg ggtgaccagg atactcatgc cggggcctct ccgttataga cttcagcgag
     1921 gatctcgcgc gcgccgttat tcagtagctc ttctgccagc gaaatcccca tttgttcggc
     1981 atcttgcggc gcaccgcggc gttcaccgcg aataatctgc gaaccgtccg gcgtgccgac
     2041 cagcgcacat agccagattt cgccatcaat aagctcggcg tagctaccaa ttggcacctg
     2101 acatccgcct tcgagacggg tattcatggc gcgttctgcg gtaacgcgca gtgcagtttc
     2161 gtggtgattc agcgcggcaa gcagctcgcg agtgcgtgta tcatcaaggc ggcattcaat
     2221 accgactgca ccttgtccta ccgccggaag agaaatctcg ggtggtaacg cggcgcgaat
     2281 gcgggactcc agacctaaac gttttagtcc ggctacggca agaatgatgg catcgtattc
     2341 gccgttatct agtttgctca ggcgagtgcc gacgttgccg cgcagggagc ggataatcag
     2401 atccgggcgg cgttcagcca gttggcactg gcgacgtaaa ctggacgtcc cgacgatact
     2461 gcctgctggt aacgcatcca gactgtcata gttattggac acaaaggcat cgcgaggatc
     2521 ttcacgctcg caaatagtga ccagccccag accttgcggg aattcaaccg gcacatcttt
     2581 cattgaatgt acggcgatat cagcgcgatt ctccaggagc gcgacttcca gctcttttac
     2641 aaataagcct tttccgccta ctttcgccag cggcgtatca agaatcacat cgccgcgcgt
     2701 caccatcggt accagttcaa cgaccaggcc cggatggctc gccatcaact tgtctttgac
     2761 atagtgtgcc tgccagagtg caagtgggct ttggcgtgtg gcaattctta aaacattgtc
     2821 taacatgctt gttaccgtca ttatcatccg tggtccatcc taacatcctt gccagagtga
     2881 tgtcagtgtt gtggtgaaac gtagacgcct gcgcaaaccg taaaatgagg tctggcagtg
     2941 gatcctgaca ggcgtttcac gccgtcgtaa taaggaattt acagagaata aacggtgcta
     3001 cacttgtatg tagcgcatct ttctttacgg tcaatcagca aggtgttaaa ttgatcacgt
     3061 tttagaccat tttttcgtcg tgaaactaaa aaaaccaggc gcgaaaagtg gtaacggtta
     3121 cctttgacat acgaaatatc ccgaatgccg cgtgttaccg ttgatgttgg cggaatcaca
     3181 gtcatgacgg gtagcaaatc aggcgatacg tcttgtacct ctatattgag actctgaaac
     3241 agagactgga tgccataaat caattgcgtg tggatcgcgc gcttgctgct atggggcctg
     3301 cattccaaca ggtctacagt ctactgccga cattgttgca ctatcaccat ccgcgaatgc
     3361 cgggttacct tgatggtaac gttcccaaag gcatttgcct ttacacgcct gatgaaactc
     3421 aacgccacta cctgaacgag cttgaactgt atcgtggaat gtcagtacag gatccaccga
     3481 aaggtgagct tccaattact ggtgtataca ccatgggcag cacctcgtcc gtagggcaaa
     3541 gttgttcctc tgacctggat atctgggtct gtcatcaatc ctggctcgat agcgaagagc
     3601 gccagttact acaacgtaaa tgcagcctgc tggaaagctg ggccgcctcg ctgggtgtgg
     3661 aagtcagctt cttcctgatt gatgaaaacc gcttccgtca taatgaaagc ggcagcctgg
     3721 ggggcgaaga ttgtggctcc acccagcata tactgctgct tgacgaattt tatcgtactg
     3781 ccgtgcgtct cgccggtaag cgtattctgt ggaatatggt gccgtgcgac gaagaagagc
     3841 attacgacga ctatgtgatg acgctttacg cgcagggcgt gctgacgcca aatgaatggc
     3901 tggatctcgg tggcttaagc tcgctttctg ctgaagagta ctttggtgcc agcctttggc
     3961 agctctacaa gagtatcgat tccccataca aagcggtgct gaaaacactg ctgctggaag
     4021 cctattcctg ggaatacccg aacccacgtc tgctggcgaa agatatcaaa cagcgtttgc
     4081 acgacggcga gattgtatcg tttggcctcg atccatactg catgatgctg gagcgtgtta
     4141 ctgaatacct gacggcgatt gaagatttca cccgtctgga tttagtacgt cgctgcttct
     4201 atttaaaagt gtgcgaaaaa ctcagccgtg aacgcgcctg tgtaggctgg cgtcgcgcag
     4261 tgttgagcca gttagtgagc gagtggggtt gggacgaagc tcgtctggca atgctcgata
     4321 accgtgctaa ctggaagatt gatcaggtgc gtgaggcgca caacgagctg ctcgacgcga
     4381 tgatgcagag ctaccgtaat ctgatccgct ttgcgcgtcg caataacctt agcgtctccg
     4441 ccagtccgca ggatatcggc gtactgacgc gtaaactgta tgccgcgttt gaagcattgc
     4501 caggtaaagt gacgctggta aacccgcaga tttcacccga tctctcggaa ccgaatctga
     4561 cctttattta tgtgccgccg ggccgggcta accgttcagg ttggtatctg tataaccgcg
     4621 cgccaaatat tgagtcgatc atcagccatc agccgctgga atataaccgt tacctgaata
     4681 aactggtggc gtgggcatgg tttaacggcc tgctgacctc gcgcacccgt ttgtatatta
     4741 aaggtaacgg cattgtcgat ttgcctaagt tgcaggagat ggtcgccgac gtgtcgcacc
     4801 atttcccgct gcgcttacct gcaccgacac cgaaggcgct ctacagcccg tgtgagattc
     4861 gccatctggc gattatcgtt aacctggaat atgacccgac agctgcgttc cgcaatcagg
     4921 tggtgcattt tgatttccgt aagctggatg tcttcagctt tggcgagaat caaaattgcc
     4981 tggtaggtag cgttgacctg ctgtaccgca actcgtggaa cgaagtgcgt acgctgcact
     5041 tcaacggcga gcaatcgatg atcgaagccc tgaaaactat tctcggcaaa atgcatcagg
     5101 acgccgcacc gccagatagc gtggaagtct tctgttatag ccagcatctg cgcggcttaa
     5161 tccgtactcg cgtgcagcaa ctggtttctg agtgtattga actgcgcctt tccagcaccc
     5221 gccaggaaac cgggcgtttc aaggcgctgc gcgtttctgg tcaaacctgg ggcttgttct
     5281 tcgaacgcct gaatgtatcg gtacagaagc tggagaacgc catcgagttt tatggcgcta
     5341 tttcgcataa caaactgcac ggcttgtcag tgcaggttga aaccaatcac gtcaaattac
     5401 cggcggtggt ggacggcttt gccagcgaag ggatcatcca gttctttttc gaagaaacgc
     5461 aagacgagaa tggctttaat atctacattc tcgacgaaag caaccgggtt gaggtgtatc
     5521 accactgcga aggcagcaaa gaggagctgg tacgtgacgt cagtcgcttc tactcgtcat
     5581 cgcacgaccg cttcacctac ggctcaagct tcatcaactt caacctgccg cagttctatc
     5641 agattgtgaa ggttgatggt cgcgaacagg tgattccgtt ccgaacaaaa tcgattggca
     5701 acctgccgcc cgccaatcag gatcacgata cgccgctatt acagcagtat ttttcgtgat
     5761 gagcgtgccg gaaagcgagg cttatccggc atgcaatctt agcggaaact gactgtttca
     5821 cccgcctgtt gcgtcgccgc ctgttccagc aaatcccaga aggtttcgcc gctgcgatcg
     5881 caaatccatt catcgccttt caggtcaaaa tggtagccgc cctgtttggt tgccagccac
     5941 acctggtgca gcggctcctg gcggttaata atgattttgc tgccgttttc aaaggtaatg
     6001 gtcagtacgc cgccgttgat ttcgcagtcg atatcgctgt cgccatccct gtcgtccaga
     6061 cgctcttcaa tggtcagcca cagttgatca gccaggcgat gaaattcact gtcgttcatt
     6121 gttgtatcct gtttttaagt gatggcggca gtatagcggc atggggtcag ggcttcaaag
     6181 tttgcacctc tgctgctgcg ttccggcatg attcatccgt caccggaata atgatgtctc
     6241 tgttttgaac ttcacgtacg ttctcctctc tgacacgagt tttcgccggg cagggggagg
     6301 gcgtaatcct tacctctgta tcggtccatg tataaacatt gcgatcgtca taagcgaagc
     6361 gatcgttttt agttagcgaa ccgttaactg gaataatttg caagctcttt gaccgtaatt
     6421 ggcggataaa gtaagcatgc tgtttgccgc tattaaatga gcgatcaatg accaggtctg
     6481 gcgtatccag cgggattatc tcaagctggt cttcaccaaa tgtcgataat tttgttacat
     6541 acagccgatc tttatattgc gcaatattgc cagtaatcag atggaatt
//
LOCUS       DAJTSA010000163         6520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000163 DAJTSA010000000
VERSION     DAJTSA010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.163"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            439..1827
                     /gene="narU"
                     /locus_tag="OO369_003869"
     CDS             439..1827
                     /gene="narU"
                     /locus_tag="OO369_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCR5983959.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1909..5649
                     /gene="narZ"
                     /locus_tag="OO369_003870"
                     /pseudo
     CDS             1909..5649
                     /gene="narZ"
                     /locus_tag="OO369_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5646..>6520
                     /gene="narH"
                     /locus_tag="OO369_003871"
     CDS             5646..>6520
                     /gene="narH"
                     /locus_tag="OO369_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5983960.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1557 a         1713 c         1691 g         1559 t
ORIGIN      
        1 cttagatgaa ataaacaata cttactataa taacgattat tacaatcagg caataagaga
       61 ggaaccgata gactttctgg acagatcgtt ttcctccaat tcatggcctt tttagtgaat
      121 aaatcatcac taattctata agtgtctggt cttgttgaga atatagttaa tatgtaaccc
      181 tttagattca cgatctgaag ggttgaaaca tgacatacgt tcaaagatta acccccgttt
      241 gaaggttagc ttgttagtta catttagtaa cacatatctg gctacttaat ccccattctg
      301 cccccgcggt tacgctttct tatctattct ttcaatcagc acttcaccca attcatcctt
      361 aattctaata actggctatt gcgccttacc tcctgttttg tcttttttca ttaccaatgt
      421 gtgcatatga ggaacaatat ggcactgcaa aatgagaaaa atagtcgtta tcttttacgc
      481 gactggaaac cagaaaatcc ggccttctgg gaaaataaag gaaaacatat tgctcgaaga
      541 aacctctgga tatcagtcag ttgtctactt cttgccttct gtgtctggat gctatttagc
      601 gcagttaccg ttaatctcaa taaaatcggt tttaatttta ctaccgatca actcttttta
      661 ttaaccgcat taccctccgt ttctggcgca ttattgcgtg ttccctactc ctttatggtg
      721 cctatattcg gtggacgccg atggccggtt tttagtactg caatcctgat tattccttgc
      781 gtctggctcg gaattgccgt gcaaaatccg aatactcctt ttgggatatt tatcgttatc
      841 gctttgctat gcggttttgc aggtgcaaac tttgcttcga gcatgggcaa tatcagtttc
      901 ttctttccaa aagccaaaca agggagcgct cttgggatta atggcggatt aggaaactta
      961 ggtgtaagtg taatgcagct ggttgcaccg ctggtcattt ttgtacctgt atttgccttt
     1021 ctcggcgtca atggcgtacc gcaggccgac ggttcggtga tgtcgctggc gaatgccgca
     1081 tggatttggg taccgctact ggcgattgcc acgatcgccg cctggtcagg gatgaatgat
     1141 atcgccagtt cacgcgcctc aattgccgac cagctccctg tcttacaacg cctgcatctc
     1201 tggctgctga gcctgcttta ccttgccacc ttcggttcgt ttatcggttt ttctgcgggt
     1261 tttgccatgc tggcaaaaac ccagttcccg gatgtgaata ttctgcgcct ggcgttcttt
     1321 ggcccattta ttggtgccat cgcgcggtcg gttggtggtg ctatttccga taaattcggc
     1381 ggcgtgcggg tgacgttgat caactttatt tttatggcga ttttcagtgc cctgctgttc
     1441 cttaccttac cgggcacagg ctccggtaat ttcatcgcct tttacgccgt atttatgggg
     1501 ctgtttctga ccgcgggtct gggaagtggt tctactttcc agatgatcgc cgtcatcttt
     1561 cgccagataa ccatttatcg ggtgaagatg aaaggcggta gtgatgagca agctcagaga
     1621 gaagccgtca ccgaaacggc ggcaacgctg ggctttatct cagccattgg cgcagtgggc
     1681 ggctttttta ttccgcaggc atttggcatg tcgctcaata tgaccggctc tccggtcggc
     1741 gcgatgaaag tgtttttaat cttctacatc gtttgtgtgc tgctgacctg gctggtttat
     1801 ggtcggcgga agttcagcca aaaataagag atcgatgcct gatgtacaac catgtcgcat
     1861 ccgacaaggc atcagatccc aattcattcc tggagcagga gtcatgtcat gagtaaactt
     1921 ttggatcgct ttcgctactt caaacaaaag ggcgaaacct ttgccgatgg tcacggacag
     1981 gtgatgcata gcaaccgcga ctgggaggac agctatcgcc agcgttggca gttcgacaaa
     2041 atcgtgcgtt ccacccacgg cgttaactgt acaggctcct gtagctggaa aatctacgtt
     2101 aaaaatggtc tggtgacctg ggaaatccaa cagaccgact acccgcgcac tcgccctgac
     2161 ctgcccaatc atgaacctcg cggctgcccg cgtggcgcaa gttactcctg gtatctttac
     2221 agcgctaacc gcctgaaata cccgctcatt cgtaaacgac tgattgaact gtggcgcgaa
     2281 gccctcaaac aacacagcga tccggtactg gcgtgggcat cgattatgaa tgatccgcaa
     2341 aagtgcctga gctacaaaca agtgcgtggg cgcggcgggt ttatccgctc caactggcag
     2401 gaactaaacc agctgattgc cgccgctaac gtctggacca tcaaaaccta cggcccggat
     2461 cgcgttgccg gtttctcgcc gatcccggcg atgtcgatgg tttcttacgc cgccggaacg
     2521 cgttatctgt cgctgcttgg cggcacctgt taaagtttct acgactggta ttgcgacctg
     2581 ccgcccgcct cgccgatgac ctggggcgag caaaccgacg taccggaatc tgccgactgg
     2641 tataactcca gctacatcat cgcttggggg tctaacgtac cgcagacacg tacgccggac
     2701 gcccacttct ttaccgaagt acgctacaaa ggcactaaaa ccatcgccat tacccctgac
     2761 tactctgaag tggccaaatt gtgcgaccag tggctggcac cgaaacaagg cactgatagc
     2821 gccctggcga tggcaatggg gcatgtgatt ttaaaagagt ttcatctcga taatcccagc
     2881 gactacttta tcaactactg ccgccgctac agcgacatgc cgatgctggt aatgctggag
     2941 cctcgcgacg atggtagcta cgttcccggg cgcatgatcc gcgcatctga cctggtggat
     3001 ggactgggcg aaagcaacaa tccgcagtgg aaaaccgtag cagttaatac cgcaggtgaa
     3061 ttggtagtgc cgaacggttc gattggtttc cgctggggag aaaaaggcaa atggaatctg
     3121 gaatccattg ccgccggtac ggaaaccgaa ttgtcgttaa ccctgctcgg tcaacatgac
     3181 gctgttgcag gcgtggcctt cccctacttt ggcggcattg aaaatccgca ttttcgcagc
     3241 gtaaaacaca atccggtgct ggtgcgccaa ttgcccgtta aaaacctgac gttagtcgat
     3301 ggcaacacct gtccggtggt cagcgtttat gatttggtac tggcgaatta cggcctcgat
     3361 cgcgggctgg aagatgaaaa cagcgcgaaa gattacgctg aaatcaaacc gtacacccca
     3421 gcctggggtg agcaaattac cggcgtaccg cgccagtata ttgaaaccat cgcccgcgaa
     3481 tttgccgata ccgcccataa aacgcatggg cgctcaatga ttatcctcgg cgcaggtgtt
     3541 aaccactggt atcacatgga catgaactac cgcgggatga tcaatatgct gatcttctgc
     3601 ggttgtgtcg ggcaaagcgg tggcggctgg gcacactatg tcggccagga aaaactgcgc
     3661 ccacaaaccg gctggttgcc actggccttt gcgctcgact ggaaccgacc accgcgccag
     3721 atgaacagca cctcgttttt ctacaatcat tccagccaat ggcgctatga aaaagtctct
     3781 gctcaggagt tactttcacc gctcgccgat gccagtaagt acagcggtca tctgattgat
     3841 ttcaacgttc gcgccgaacg tatgggctgg ctaccttctg cgccgcagct ggggcgtaac
     3901 ccgctcggga ttaaagctga agccgacaag gccggattat cccccacaga atttaccgcc
     3961 caggcgctga aatcgggcga tttacgtatg gcctgcgaac aaccagatag cggcagcaat
     4021 catccgcgta atttgtttgt ctggcgctct aacctgcttg gctcctccgg caaaggccac
     4081 gagtatatac agaagtatct gctggggacc gaaagcggga ttcagggcga ggaactcggt
     4141 gccagcgacg ggatcaaacc ggaagaagtc gagtggcaaa ctgcggcgat tgaaggcaag
     4201 ctcgacctgc tggtgacgct cgacttccgc atgtccagta cctgcctgtt ctccgatatc
     4261 gttctgccca ccgccacctg gtatgaaaaa gacgatatga acacctcgga tatgcatcca
     4321 tttattcatc cgctttctgc ggcggtcgat ccggcctggg aatcacgcag cgactgggaa
     4381 atctacaaag gtattgccaa agcattttcg caagtgtgcg tgggccatct tggcaaagaa
     4441 accgacgtgg tattacaacc actgctgcat gactctccgg cagagctctc acagccgtgt
     4501 gaagtgctcg actggcgcaa aggcgaatgc gatctgatcc cgggtaaaac cgcgccgaat
     4561 attgtggcgg tggagcgcga ctaccctgct acgtatgaac gctttacctc gctcgggcca
     4621 ttgatggaca aacttggcaa tggcggtaaa gggatttcgt ggaataccca ggatgaaatc
     4681 gatttcctcg gcaaactcaa ttacaccaag cgtgatggcc cggcacaggg gcgtccgctg
     4741 attgacaccg ccattgacgc ttcagaagtg attctggcac tggcaccgga aaccaacggt
     4801 catgttgcag tcaaagcgtg gcaggcgctg ggcgagatca ccgggcgcga acatacccat
     4861 ctggcgctgc acaaagagga cgagaagatt cgctttcgcg atattcaggc gcagccgcgt
     4921 aaaattatct ccagccccac atggtccggt ctggaaagcg atcacgtatc ctataacgcg
     4981 ggatacacca acgttcatga gttaattccg tggcgcacgc tgtcgggacg ccagcagctc
     5041 tatcaggatc atccgtggat gcgtgctttt ggtgaaagcc tggtggctta tcgcccgcct
     5101 atcgacaccc gtagcgtcag tgagatgcgc cagataccgc caaacggctt ccaggaaaaa
     5161 gcacttaact tcctgacgcc gcaccagaaa tggggcattc actcaaccta cagtgaaaac
     5221 ctgctaatgc tgacgctctc tcgcggtgga ccgattgtct ggatcagcga aacagatgcc
     5281 cgagaactga ccattgtcga taacgactgg gtggaagtat ttaacgccaa cggcgcgctg
     5341 actgcccgcg cggtggtcag ccaacgtgta ccgccgggca tgaccatgat gtatcacgcc
     5401 caggaacgca ttatgaatat tcctggttcg gaagtaactg gcatgcgcgg cggcattcat
     5461 aactcggtca cccgcgtttg cccgaaaccg acgcatatga ttggcggtta cgcgcagctg
     5521 gcctggggct ttaactacta cggcaccgtc ggttcgaacc gcgacgaatt catcatgatc
     5581 cgcaagatga agaacgttaa ctggctggat gatgaaggtc gcgatcaggt acaggaggcg
     5641 aaaaaatgaa aatacgttcg caagtcggca tggtgcttaa cctcgataaa tgtatcggct
     5701 gccatacctg ttcggtgacc tgtaacaacg tctggaccgg acgcgaaggc atggagtacg
     5761 catggtttaa caacgtcgaa accaaaccgg gcattggtta tccgaaaaac tgggaagatc
     5821 aggaagagtg gcaaggcggc tgggtccgcg atgtgaatgg caagatccgt ccgcgtctgg
     5881 gcagcaagat gggcgtaata accaaaatct tcgccaaccc ggtggtgccg cagcagattg
     5941 atgattacta cgaacctttc accttcgact acgaacattt gcatagcaca ccggaaggca
     6001 aacatatccc tactgctcgc ccgcgttcac tgattgacgg caaacggatg gacaaagtga
     6061 tctggggccc aaactgggaa gaactgctgg gcggcgagtt tgaaaaacgt gcccgcgacc
     6121 gcaacttcga ggccatgcaa aaggagatgt acgggcagtt tgaaaacacc ttcatgatgt
     6181 acctgccgcg cctgtgcgaa cactgcctca atcccagctg cgtggcgacc tgcccaagcg
     6241 gcgctatcta caaacgcgaa gaagacggca ttgtgctgat tgatcaggat aaatgccgtg
     6301 gctggcgttt gtgcattagc ggttgtccgt acaaaaaaat ctacttcaac tggaaaagcg
     6361 gcaagtcaga aaaatgtatc ttctgttatc cgcgaattga gtcaggccaa cccaccgtgt
     6421 gctcagaaac ctgcgtgggt cgcatccggt atctgggcgt gctgctttac gacgccgacc
     6481 gcatcgagga agcgtcgagc accgagcgcg aagttgacct
//
LOCUS       DAJTSA010000164         6374 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000164 DAJTSA010000000
VERSION     DAJTSA010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.165"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            73..378
                     /locus_tag="OO369_003872"
     CDS             73..378
                     /locus_tag="OO369_003872"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5983961.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /locus_tag="OO369_003873"
     CDS             complement(454..918)
                     /locus_tag="OO369_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5983962.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="OO369_003874"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="OO369_003874"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR5983963.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="OO369_003875"
     CDS             complement(1787..2476)
                     /locus_tag="OO369_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR5983964.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="OO369_003876"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="OO369_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR5983965.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="OO369_003877"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="OO369_003877"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR5983966.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="OO369_003878"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="OO369_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR5983967.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1619 a         1716 c         1579 g         1460 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcaggatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaatcacct cacctacaat taaaaacacg acatccgcac cataaataga cttgcaaaaa
     6241 atataacatc attgttttca atctgccatt tatgggattg accgttttct tttgacacgg
     6301 agttcaacaa tgggtaatgc ttccaactta ctgatttagt gtatgatggt gattttaagg
     6361 tgcttgcgtg gctt
//
LOCUS       DAJTSA010000165         6321 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000165 DAJTSA010000000
VERSION     DAJTSA010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.166"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            103..282
                     /locus_tag="OO369_003879"
                     /pseudo
     CDS             103..282
                     /locus_tag="OO369_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            807..1985
                     /gene="ltrA"
                     /locus_tag="OO369_003880"
     CDS             807..1985
                     /gene="ltrA"
                     /locus_tag="OO369_003880"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5983968.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     NGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            2068..2247
                     /locus_tag="OO369_003881"
                     /pseudo
     CDS             2068..2247
                     /locus_tag="OO369_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2277..2759)
                     /locus_tag="OO369_003882"
                     /pseudo
     CDS             complement(2277..2759)
                     /locus_tag="OO369_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(3015..3995)
                     /locus_tag="OO369_003883"
     CDS             complement(3015..3995)
                     /locus_tag="OO369_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR5983969.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3995..5194)
                     /locus_tag="OO369_003884"
     CDS             complement(3995..5194)
                     /locus_tag="OO369_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5983970.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5516..5854)
                     /locus_tag="OO369_003885"
                     /pseudo
     CDS             complement(5516..5854)
                     /locus_tag="OO369_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5921..>6321)
                     /gene="istB"
                     /locus_tag="OO369_003886"
     CDS             complement(5921..>6321)
                     /gene="istB"
                     /locus_tag="OO369_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCR5983971.1"
                     /translation="RVGIKVRFTTAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIID
                     EIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRIL
                     HHSHVVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT         1716 a         1460 c         1508 g         1637 t
ORIGIN      
        1 actcctccgg cagaagcaga aaaagcttat tatgcttcca tcggaaacga tgatctggca
       61 gcctgagttc acagataaaa cactctccag gaaacccggg gcggttcagt tgctgaactg
      121 ctggaaccgc tgtatgacat cctgcgccag tatgtgctga tgcccggtaa agtccatgcc
      181 gatgatatcc ccgttccggt ccaggagccg ggcagcggta aaacaaggac tgcccggctg
      241 tgggtctacg ttcgtgatga ccgtaacgcc ggttcggaaa ttgcagcata agttatccat
      301 gctctgaggt gggtagaggt gggccctccg gtcagggttt acaggagcac agaccaaacg
      361 acccggtgct gcttcattct aaggaatgag gtggtgagcg actcgggaaa aggcagagat
      421 ctactgtcac gtgggttcgg gaagctgaac gaaagtaaac tgtccgatga agcatcgaaa
      481 tcttagatga tgccaaaatg gggccgtaca gccgtcccct gataagcgca gaagacacct
      541 gcttactgac tgcgcggcat ccggtgtgaa ggcagcagga tcccgggcca gaccatctca
      601 gggaacgtga gaacctgttt ccggcacgta tcgggaaaca ggggcggacc cattcgtagt
      661 agtgatgaaa gatctgtaat gggtctggag cgaagggatg gggatcacat tcattgcaga
      721 agacacaacc caaaagggga tgacgtaact gatgcaacga aaatcatttg aaataccaaa
      781 agcactggtc tgggcatcat atctggatgt gcgtcgcaac aagggagccc cccggatgcg
      841 acgggcagac tttgaaaatg ttcgaccagc aacgtgatgg caatctatac aagatctgga
      901 accggctatg ttcgggaaca tggtttccgc caccagtgct ggaaaaacgg atcccgaagt
      961 cgaatggcaa ggaacgcatt ctggggatcc caacagtatc cgatcgaatt gctcagggag
     1021 cgataaaact tttcatggaa gaaaagcttg acccgatttt ccatgctgat tcatatggtt
     1081 accgaccagg caaatcagct catgacgctc tgaaacaatg tgccatccgg tgctggcgtt
     1141 atagctggat actggaagtg gacatcagtg cgtttttcga ccatgtgaga catgatctgg
     1201 tgctcaaagc tctggaacac cacgggatgc ctaaatgggt catcctgtac tgtcgacgct
     1261 ggatggaagc gccaatgcag agttgtgaaa atggagaatt aataacccga acacgaggca
     1321 ctccgcaggg cggggtcatt agtccgctac ttgctaactt gttccttcac tatgcatttg
     1381 atttgtggat ggaaagagaa tatcgggggg taccgtttga gaggtacgct gacgatattg
     1441 tagtacattg ttcacgaatg agtgatgcaa caaggctaaa gaacaggtta tcggagcgct
     1501 tctcggaagt cgggctggtc ctgaatgcag ggaaaacgaa tatcgcctac attgacacgt
     1561 ttaaaaggag aaacgtcgca acgagtttca cctttctcgg atatgacttc aaagtgcgta
     1621 cgctgaagaa tttcaacggc gaacggtacc gaaaatgcat gccgggtgcg tcaaatgcag
     1681 caatgcgcaa aataacagaa acaatcaaga agtggcgtat acatcgctca acagctgaga
     1741 gtttgctgga ttttgcgagg cgctacaatg cgatagtgag aggctggatc gagtactacg
     1801 gaaagttctg gtccagaaat ttcaactatc gactgtggag tgcaatgcag tcacgtctgc
     1861 tcaagtggat gcagtctaaa tacagacttt cgaaccggaa ggctcagcga aagctgacgc
     1921 tggtaaggaa ggagtatccg aagctatttg ttcactggta tttactacgt gcatcaaatg
     1981 agtgatcaag agccgtatga cgcgagagtg tcacgtacgg ttccgtgagc agccggggag
     2041 tgaaattctc tccggctgac tcgactcgcg ggttccgaca gcgatgacga acatgcggcg
     2101 gtgttgtact cgctgatcgg cacatgccgt ctgaacaatg tggaaccaga aaaatggctg
     2161 cgttacgtca ttgagcatat ccaggactgg ccggcaaacc gggtacgtga tctgttgccc
     2221 tggaaagttg atctgagctc tcagtaaata tcaatacggt tctgacgagc cgcttacttt
     2281 tggcgtaatt ctgctgcgcc tttactgaac acaatgccgt attcagcaag aagtccacga
     2341 atgtgattta tcagttgtgt cctttccatt gaaaccaacc gacgcatatt caaaaaagtc
     2401 tggacgtcct gttgctccag agtttttatt ggtacgggtc gtatagttcc atgttggcag
     2461 gcctcagcta ttgcctgtgc gtcgttataa tcatttttct ggcaccgctg ataagcttta
     2521 acatgttgtg ccggaagcaa tattacatca tggtcgagtt tcctgatttc ccgggcccag
     2581 taatgagatg ccccgccagc ctccatcgca acagtcattt taggctgttg caccagccag
     2641 ttaagcagtt gattgcgaga aagtttggtc tttcctgcgg gtttgccatg agcattgata
     2701 ctgaaaagat aaaagttcgt tttagccaga tcaatgccgg tgattttctg ttcagacatg
     2761 gtatcccgct tacttttgtt gttaaagttt atccatcatg gcgcaattga aggctgccgt
     2821 gaaagagggg gaagactatc tcatcggtta ctgttaaacg gctaagagaa actatctttt
     2881 tcaagcaaag aagatacggt gaaacaggcg atttcaccgt gaaatactgt tcaaaaaaaa
     2941 cgctaataaa agcaaaattt cttttaaaac agccaattaa aaactggcga gttgctggcg
     3001 aaaaacttga attttcacag cgaattcaga ttatctttta gtacaagagc aatcatacga
     3061 tctaacttat cctgaacttc caacggtaat ctgccgaatt catatgagaa cgtacgtccc
     3121 tttactcgtt ttcgagcaaa tttatcttta ctatcaaatt tccataacaa agttacaacc
     3181 gctttatcct ttactcctga atcaagcaat gaagacattt ccttggttat aagccgcata
     3241 atactgtttt taacctcgtc ttcagaaaga ttgtcatcag cagtaataag ctcaatactc
     3301 ggcgagatat tcttcacaac ttcatcaata gatatatcac ccagcctcag tcgttcactt
     3361 aattcagcca gttgcctgta atcggcgtat gtcagctcag actgaacagg aaaaagagac
     3421 acaaaatctt taggtacgga cgctgcttgg agggcacgag tcaccttagc tgccgacaat
     3481 ccctctcttt ccgcaatttg cttctggttc ataccggcct ctttgagacg tactaaacgc
     3541 aacccaattt cacgaatatt atgttcaagt gacgtctgca aatctttcgc cagatgctga
     3601 gcttcagaaa cagtaagctc atcatccgtt acaagtacac gtaatccaac cttgcatagc
     3661 aaagctgctg cccgacgacg ggaaccatcc aaaatctcaa taagatcgcc agtccgaatc
     3721 ccaatacatg gatagaattg ttgtaaacgt atagttctgg tgatgtcttt tagtgagtcc
     3781 ggcgtcaggg ctgtctgttc gcggccattc acctcttcaa ccacaaaagt ttgagtatcc
     3841 actttgtcat gatcaacctt gatctcagta aattttgcct ttcggccagt attgagggta
     3901 aatacatgaa ctggagccga tatttcttca gtattattca ggatatttgt attcagcgta
     3961 cggccaatag tcggtcggtg ctttctgttt tccatcagta gttggccctt ataaattcaa
     4021 tacgatcaaa aactgcttta gcgaaatctt ctgctgccat acgtgcattt ttcaaagctt
     4081 caccactgcc aacataagta acaggattcg cagatatcac ggtatcaaac gactcgccgc
     4141 ttctctcaaa accatcaaga cgtggcattg aaacatcaag catgtcacca ccgaaaatct
     4201 cttttgtcag gctatggcag tatttatgat ctgacttatt cgccagtttc gacatgaagc
     4261 caatatttgc ctgcaaacgg catgaacatc catcctgttc aataatctgt acaagttcag
     4321 gtaaacgagc caaatattta agcgtggaat ggaaatcaac ttgtgctggc ggcaccggtg
     4381 taaacatgat atctgcggca gcaatggcat ttttcaaaaa tgcgtccaga tgcgggcctg
     4441 tatcaataag gatgaagtcg aaatcatgct tcagcttatc aataatattc tcacgcaata
     4501 tcgcgtgttt gttttgtcct aatagatgct cctcgcacag agtatcccag tttgatgcaa
     4561 taaaggcgtc gtcaatagaa gccggcataa cataaacgcc agggataact gacggaacga
     4621 taaaatcttc cagtaattcc tcacgactca cgttctgcaa catagcctgc ggtgcggttg
     4681 tatcaaccag ccccaccgca tgcaagtagt tcagaaacat agtggctgat gactggggat
     4741 ctaaatcaag gagcagaata cgtaaatctt ctgacagcag gtgaggatga gcacgcaggg
     4801 cgtgagctac ggaaacacta ctcacggttt tagacacacc gccttttaga ctaccgatga
     4861 atatggaata tgcctcacta taacgatcgc gatatttagg aatgccacga tgtgcataga
     4921 tatcgataat gttctggatg gttaatgcat attttgtcgc ggttcctgct gggcgttttt
     4981 caaactgata gccctgagcc tccatctcac cgactgcacc atccacgctt gcgcgcgtta
     5041 attttggaag cttagctaca gcagactttg aatagacttg ataaaaaaca tcagcctgaa
     5101 actcctgctt ttgctcctga atctgttttg ttagggcgag caacatttta tctgcacgct
     5161 gcgcaacctt acttaactgc tcaaaactag tcattccccc tccgtgctat gtaggatttt
     5221 aacttattac aaaaaaaact gcattagcag ggtaatgcat gcaagtacat attgtacatg
     5281 cttatattaa aaatgcgcgc attgctgatt tattaatcat cggaatgaat attactgtaa
     5341 gatagtatgc ctttccggga gaaagacgct gatttccccc gaataatcca gttcaccacc
     5401 acactccgga caacagcttt cttcaggctc cagatggtgt atttcgcggg aaacatgttc
     5461 cggtaacgga cggcgatgac gtgactgtcg caacaggcgg gggtagtgct tccaatcagt
     5521 agaacatgtg cttttcaatg aaggagccga tgactttttc gtggatttcc acggaacgcg
     5581 agaagcagat tgttttacgt gccagccgct tgatgcgggt gcggagggtc cggttgttac
     5641 gtgcaatacg ttgcgtgaag attttgccgg tcaggtgttt ctccttcggc acttctctgg
     5701 cgtagctgcc ccagtcatcg ctggtcacca tgccgataca gaacggagtc aacagggcta
     5761 acagttcccg acaggtctca tcattccgtg gcccgaaagt gtaagccaac acaccgccag
     5821 ttttagtgtt gtacgcatac cagagccagt gttgtgtcaa cgacggatga aaagtgatcc
     5881 acttatatct ccaccaacgg cccaatattg atccaccgtt ttactcagga ttagcttctg
     5941 ctataacccc ggcctttcgt ttctgtctga gtcgatagct ttctcctttg atttgaacga
     6001 catgtgagtg gtgtaagata cggcccagca tcgctgaggt cagtgctgca tcaccggcga
     6061 acgtttgatc ccactgcccg aacggtagat tggatgtcag gatcattgcg ctcttttcgt
     6121 aacgtttagc gatgacctgg aagaacagtt ttgcttcttc ctgactgaac ggcagatagc
     6181 ctatttcatc aatgatgagc aggcgggggg ccattactcc acgctgaagc gtcgttttat
     6241 aacggccctg acgttgtgcc gtagataact gaagtaacag atctgctgct gttgtgaagc
     6301 gaactttgat acctacacgg a
//
LOCUS       DAJTSA010000166         6161 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000166 DAJTSA010000000
VERSION     DAJTSA010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.167"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..171
                     /locus_tag="OO369_003887"
     CDS             <1..171
                     /locus_tag="OO369_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5983972.1"
                     /translation="IRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP
                     KRYRKKLTEFNQ"
     gene            276..1628
                     /gene="celF"
                     /locus_tag="OO369_003888"
                     /gene_synonym="chbF"
     CDS             276..1628
                     /gene="celF"
                     /locus_tag="OO369_003888"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR5983973.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1641..2399
                     /gene="chbG"
                     /locus_tag="OO369_003889"
     CDS             1641..2399
                     /gene="chbG"
                     /locus_tag="OO369_003889"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR5983974.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAIPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2446..4707)
                     /gene="katE"
                     /locus_tag="OO369_003890"
     CDS             complement(2446..4707)
                     /gene="katE"
                     /locus_tag="OO369_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR5983975.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4890..5153
                     /gene="cedA"
                     /locus_tag="OO369_003891"
     CDS             4890..5153
                     /gene="cedA"
                     /locus_tag="OO369_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR5983976.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5430..6161
                     /locus_tag="OO369_003892"
                     /pseudo
     CDS             5430..6161
                     /locus_tag="OO369_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1580 a         1311 c         1557 g         1713 t
ORIGIN      
        1 atccgtatta attttgcaaa aaaacaactg gaaatgacca actattccgt gacggatatt
       61 gcgtttgagg ccggttatag tagcccgagt ttgtttatta aaacgtttaa gaaattaacg
      121 tcctttacgc ctaagagata tcgtaagaaa ttgactgaat ttaatcagta agtcgttata
      181 cctgacaatt cacatatctg tcctgttgct ggattattta tgtccggggg ggcagatatg
      241 ccagatatca gtattctgta ctgaagggag aaattatgag ccagaaatta aaagtcgtca
      301 ctattggtgg cgggagcagc tataccccgg agttactgga aggatttatt aagcgttatc
      361 acgaattgcc ggtcagcgaa ttatggctgg tggatgtcga gggtggcaaa gctaaactgg
      421 atattatttt cgatctctgc caacggatga ttgataacgc tggcgtcccg atgaagcttt
      481 ataaaacgct ggattgccgc gaagcattga aagatgctga tttcgttact acccaactgc
      541 gcgttggcca attaccggcg cgtgaactgg atgaacgtat tccattaagt catggttatc
      601 ttggtcagga aaccaacggc gcgggcggtt tgtttaaagg tctgcgtacc attccggtga
      661 tttttgacat cgtaaaagat gtcgaagaac tttgtccgaa tgcatgggtg attaacttca
      721 ctaacccggc gggaatggtt actgaagccg tttatcgtca taccggattt aaacgcttta
      781 tcggcgtgtg taatattccg atcggcatga agatgtttat tcacgatgtt ctgatgctga
      841 aagacagcga tgatttatct atcgatttgt ttggcctcaa ccatatggtg ttcattaagg
      901 atgtgctggt aaatggcaag tcgcgctttg ccgaattgct tgatggtgtg gcatccgggc
      961 agttaaaagt atctggcgtt aaaaatattt tcgatctgcc atttagcgaa ggcttaattc
     1021 gttctctgaa tctgttgccg tgttcttatt tgctttatta cttcaagcaa aaagagatgc
     1081 tggctattga aatgggcgaa tactacaaag gcggcgcacg agcgcaggta gtacagaaag
     1141 tcgagaaaca actttttgag ctgtataaaa acccggagtt gaaagttaag ccgaaagaac
     1201 tggaacagcg cggtggggct tattactctg atgcagcgtg cgaagtgatc aacgctatct
     1261 acaacgacaa gcaagctgaa cattacgtta atatcccgca tcatgggcat attgataata
     1321 ttccggcaga ctgggcggta gaaatgacct gtaagctggg gcgcgatggc gcgacgccac
     1381 atccgcgcat tacgcatttc gatgataaag tgatggggct gattcacacc attaaaggct
     1441 tcgagattgc tgccagcaac gccgcactta gcggagaatt taacgatgtg ttactggcgc
     1501 taaaccttag tccgttggtg cattccgatc gcgatgctga gctgctggca cgcgagatga
     1561 ttctggcgca cgagaaatgg ctgccaaatt ttgccgactg catcgcagag cttaaaaaag
     1621 cacattaacc gaggctgatt atggaacgct tactgattgt taatgccgat gattttggct
     1681 taagcaaagg tcagaactac ggcattatcg aggcctgtcg caatgggatt gtcacatcga
     1741 cgacggcgct ggtgaatggg caggctattg accatgcggt gcagttgagt cgtgatgaac
     1801 cgagtctggc catagggatg cactttgtcc ttactatggg caagccactg acagctatac
     1861 cggggttaac ccgcgatggt gtgctgggaa aatggatctg gcagttggca gaagaagatg
     1921 ctttaccgct ggaagaaatt actcaggagc ttgccagtca gtatttgcgt ttcattgagc
     1981 tatttggacg caaacctacg catcttgata gccatcatca tgtgcatatg ttcccgcaga
     2041 ttttcccgat tgttgccagg tttgcggctg aagaggggat tgcgttgcgc atcgaccgtc
     2101 agccgctatc taacgctggt gatttaccgg ctaatctgcg cagttcgcag ggattcagta
     2161 gcgcgtttta tggtgaagag attagcgagg cactgttcct gcaagtgctg gatgacgcaa
     2221 gccatcgggg tgatccttcg ctggaggtga tgtgtcatcc ggcgtttatc gataatacta
     2281 tccgtcagag tgcttactgt ttcccacggt taacggagct ggaagtgctg acatcagcgt
     2341 cgttgaaata tgcaattgct gagcgtggtt atcggttggg tagttatctt gatgtgtaag
     2401 taaggtggta aggtcactcc ccaggggacg tgaccttatc catatttacg ccggaatttt
     2461 gtcaatctta ggaatgcgtg accacacgcg gtgttctgcc atcagcgtta acagttcatc
     2521 cataaaacta ccgtcggtgc tgtcagcttc cacaatccct tcttcaccct ggtcagctac
     2581 cttgattgtt gctttaaact tgcgcgcgtc tcccgccagc gcaatcggtt taaggtgttt
     2641 gtaggcttcc atcagatagt agttggcatc gccgttgtca gcgatatccg cgatattgcc
     2701 gcaagggaca atgaccgcat cgaccgtcag cgaaggtgca ccggcaaagg tagcggctat
     2761 aggcaacacc gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga gcagtttggc
     2821 atgaacgcct ttggccttca tggccttgag aatggccaga aggtctgccg atctcacttc
     2881 atcattaagt aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa tggcgtacaa
     2941 acttaaggat ggatcctttt tcagaccgtt gacgtccgga ggtggggtga tattcagctg
     3001 gtcgtcagtc agttcgatac cgagattttt cgccaccgcc tgggccagag tgagatcaat
     3061 atgcgccagc tggtcaacaa cgcgctcacg aatatacgga cgaacgactt tgcttaactc
     3121 aaaactgaaa ccatcgacaa tatggcgctg ctcgaatggc gtctgactta gccagaacag
     3181 acgcggatgg gaataatatt cgccaaacga tgggctgcgc tcgcgaactt tattgccttc
     3241 cacgcgctct tggtatgatt caaaaccgcc gcgtttcggc cccggtggtg tttcgcgcgg
     3301 ccagttatcg ttgatcgagt tcggttcata attcgccggg ttagtgtcga tccccatacg
     3361 atgcatgccg tcacgctgga aattatggta agggcaggtc gggcggttaa tcggaatctc
     3421 atggaaattc ggcccaccaa gacgactgat ttgtgtatcg gtataggaga acaaacgtcc
     3481 ctgcaacagg ggatcgttgg tgaagtccag accaggaaca atatgacctg gatggaatgc
     3541 tgcttgttcg ttttcggcaa agaagttatc cggattgcga ttgagcacca ttttgccgac
     3601 acgctgaacg ggcaccagtt cttccgggat cagtttggtt ggatcgagaa gatcgaagtc
     3661 gaacttgaat tcatcttctt caggaatcaa ctggaagccc agttcgtatt ccggaaaatc
     3721 gcctgcttca atggcttccc acaactcgcg gcggtggaag tccgggtcac gtccggtgag
     3781 tttttgtgct tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc agtggaaacg
     3841 tacaaacgtt gccttccctt cggcattaat caggcggaag gtgtgaatac cgaagccttc
     3901 catggtgcgg taactgcggg ggatgccgcg atccgacatc gcccacatca cgttgtgcag
     3961 agtttcaggt tgcagagaaa cataatccca gaaagtatcg tgggcacttt gcccttgtgg
     4021 aattgcccag tgcggttctg gttttaccgc atgaacaaaa tcggggaatt tatgcgcatc
     4081 ctggataaag aagattggcg tgttattgcc aacgaggtca aaaataccct cttcagtata
     4141 gaacttggtg gcaaaaccac ggatatcacg cacggtatcg gcagagccag cgccaccctg
     4201 aacggtagag aacctaacaa atactggggt gattttgttc ggatctgaga ggaaatccgc
     4261 tttggtgata tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg ctgagccgcg
     4321 tgcatgaaca atacgttccg ggatgcgctc atggtcaaag tgggtgattt tctcgcgcag
     4381 aataaaatct tccagcagcg ttggaccacg gttaccggca cgcagtgagt tttgatcatc
     4441 ggcgatgcgc acgccctgat tagtggtcag cgcataattt tcactgcctt tgcgtacgtc
     4501 ttccagagaa ttaagttttt cgttacgtgt atcaggggct ttcaggctcc caggggcggt
     4561 aggttgtgca cctggcggtg ttggttcagc cgctggacga tgagatccgt cctcaggtgc
     4621 cagtgagtcc atccccggtt tcgcttcgct ggaatcgtgt agtggtgact ggtgctgatg
     4681 tgggttcttt tcgttatgtt gcgacattga actcgtctcc ttaatttatt actgaaaggg
     4741 ccgctgtttt ggtcaaaaaa ctctctaact atagaccacc agccagatcc cgcttcggag
     4801 actattctgg aaacggtgtt atgaaataca cgcaaagcgg gacgtacagg ggctaaatcg
     4861 gctaaactac agtttttcta atttttaata tgcgtttagt gaagccagta atgaagaaac
     4921 cgctccgcca gcaaaaccgc cagattatta gctatgtccc acgcacggaa cccgcgccgc
     4981 cagaacatgc gataaagatg gattcgtttc gtgatgtctg gatgctgcgt ggcaaatatg
     5041 ttgcgtttgt actgatggga gagtcatttc tgcgctcacc ggcgtttact gtgcctgaat
     5101 ctgcccaacg ttgggcaaat cagatccgcc aggaagggga agtgactgag taaggattac
     5161 atacctcccg gttttgccgg gagttacagg acctgaaatt ctgcatttcg ttgctacatt
     5221 cattcaaacc acctgtaaca aaaagctgcc tttgacgagc tattgctata aataagagat
     5281 cttttttctt taattattta tttattttaa aatggttaag ttttgctaat catgatgagt
     5341 tatttttgat agttcatcct gtggcgtcgc tcttaaactt atattgtaaa tgacttagat
     5401 ttaaataaga taataatttt gttaacaata tgaggcggga gatgttttct acaaacttaa
     5461 tacagagtaa ttatggtgat ttaaatatta aaagcctggc tttcgactct tttaaagaaa
     5521 ggcttgagtc taccatggcg gcactcactt tctttatatc aacgggtcaa tgtgactgcg
     5581 atgaaatagc agagtcgaac ttcaatcgta tgattgccta tatgtctaac attaactatg
     5641 atgcctcaaa gctaggagcg ccagcactta gttttgatac gtatctgcag gacaatgtta
     5701 aatatagagt gataattaat aatctttatg gcagtgagtt tcggataaga gatattaatg
     5761 agaattttgt tgggatggat tttacctgtg aattccgacc cgaaaaaatg acgaatttga
     5821 tcaatataaa aaataggttg gttattcatt atttaaggac tatatactat gaaaaaaatt
     5881 atatacatcc tgccgggagc atttttgctg cgattcaaaa aaaatgagag tctcctaaag
     5941 tttccttcga taatatcaat gcttaatatc aatcttcttt ttaatccact aaatcttccg
     6001 ggaatgggaa gtggtgtgct tgaagatatt atgagtattc cagatagttc acttagaaaa
     6061 cgtctcgggt atgaggtttt aagcttctca ctacaggcac attcattgag ccaggaatgt
     6121 attgataagt tggacatttt tttcgctgac gatctgttta a
//
LOCUS       DAJTSA010000167         6058 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000167 DAJTSA010000000
VERSION     DAJTSA010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.168"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..143)
                     /locus_tag="OO369_003893"
     CDS             complement(<1..143)
                     /locus_tag="OO369_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HCR5983977.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQ"
     gene            379..651
                     /locus_tag="OO369_003894"
     CDS             379..651
                     /locus_tag="OO369_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR5983978.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(617..1024)
                     /gene="nudG"
                     /locus_tag="OO369_003895"
     CDS             complement(617..1024)
                     /gene="nudG"
                     /locus_tag="OO369_003895"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR5983979.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            1111..1731
                     /locus_tag="OO369_003896"
     CDS             1111..1731
                     /locus_tag="OO369_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5983980.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1740..3047)
                     /gene="ynjE"
                     /locus_tag="OO369_003897"
                     /pseudo
     CDS             complement(1740..3047)
                     /gene="ynjE"
                     /locus_tag="OO369_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(3114..3767)
                     /locus_tag="OO369_003898"
     CDS             complement(3114..3767)
                     /locus_tag="OO369_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5983981.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3767..5238)
                     /locus_tag="OO369_003899"
                     /pseudo
     CDS             complement(3767..5238)
                     /locus_tag="OO369_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5211..6058)
                     /locus_tag="OO369_003900"
                     /pseudo
     CDS             complement(5211..6058)
                     /locus_tag="OO369_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1427 a         1621 c         1599 g         1411 t
ORIGIN      
        1 atctggcgat attttggatt ttgttcaaga aaaggccaga gtgtggtcat tacttcacga
       61 acggcttgcg cgaactcggt ttgattcggg tcgcgctttt ggacatggtt gaggaatgac
      121 tccagagaat atgtctgatc catagatata aaaaccttat atattaatac gattgtgctt
      181 atgttgttta tgtcatgtgc ttttgcagtt ttcgactata ccatcaagaa tgtgacggta
      241 agcaagaaaa aaatgaccca ggaaagcaaa ttaataacga gagtaatctc ataaccaaat
      301 gcgataactc agacgctaaa gttttgccac ccggctaccg ttatcagtag cagagacaat
      361 aacgttaagg agttccctgt gagtcgagca ttgttcgccg tagtattagc atttccatta
      421 attgcactgg ctaacccgca ttatcgtccg gacgtggagg taaacgtacc gccagaagtg
      481 ttcagttcag gcgggcagtc agcgcagccc tgtactcagt gctgcgttta tcaggatcaa
      541 aattattctg aaggggcggt aattaaagcc gaaggaatac tgctgcaatg ccagcgtgat
      601 gacaaaaccc ttagcactaa tccgctgctc tggcggcgcg taaagccata aacgcctcta
      661 ataatggaat gtcagcaggg gccagcggat attgcagcgc ctcttcaggt gagcaccaga
      721 ccagcgcctg atgttcatgt gcctgtaacg tcccgtggaa gtcgggtacg tgccaggcat
      781 gaagatggat aatccggccc gaaacttctc gctgatggct ggcaacatac tcacccacag
      841 ttgcttcgat gtccaattct tcgtttaact cacgcaccag cgcctgctgc tggctttcat
      901 ccagctcgac tttacctccg gcaaactccc ataatcccgc ctgatcgctc tgggcggggc
      961 gttgcgcgag taaaattttg ccatcacgtt caatgatggc ggcaacaact tcaatcattt
     1021 tcataaatta cacacgggca gataagcgaa aaatgccata atatccgcac tgttgctcaa
     1081 tcagaaaggc tttatattta aggagtgcct gtgctagacc gccatcttca tccccggatt
     1141 aaaccgttgt tgcatcagtg cgtgcgggtt cttgataaac cgggcattac gccagatggt
     1201 ttaacattag tgggatttgc catcggcgtg ctggctctgc cgtttctggc gctgggctgg
     1261 tatctggcgg cgctagtcgt cattttgttg aacaggctgc ttgatggtct ggacggtgcg
     1321 ctggcgcggc gcagagggct taccgacgcg ggcggctttc ttgatatttc tctcgatttt
     1381 ctcttttacg cgctggtgcc gtttggcttt attcttgctg caccggagca aaatgcgctg
     1441 gcaggcggct ggttgttgtt tgcgtttatc ggcacgggca gcagttttct cgcctttgct
     1501 gcactggcag caaaacatca gattgataac cccggttatg cacataagtc gttttactat
     1561 ctggggggat taaccgaagg cacagaaacg atcttactgt ttgtgctggg atgtttattc
     1621 cctgcatggt ttgcgtggtt tgcgtggatc ttcggtgcgt tgtgctggat gacgaccttt
     1681 acgcgggtgt ggagtgggta tctgacgctg aagtcgctcc agcgtcagta attgcaatgt
     1741 tatttgctac tgcccgggcc gcgttcaccg gttgcaaccg gattttttgg atcgctgctc
     1801 cattcgtacc agccgccgtc atacacggaa acattcttcc aacccatggc gcgtgcgtac
     1861 ataaaggttt cggacgcgcg ccagccggtg ccgcagtaga atgaaacttg ctgatctggt
     1921 ttgatattcc atgctttcca catagcggta atatcatcgg cgctacgcat ggtgccatcc
     1981 gggttataga aatcttccat atgcgtcgag tcgctaccag cgtgtcccca acgtgctccg
     2041 gctatttcac cttttggttt aatatagctg taaccgctgg tcgtaccgat aaattctggc
     2101 cacgaacgaa tgctcaccag cgatgcatcc tggcgatgca gcagtccacg ggcctgttcc
     2161 atatcgagca tcaactgcgg ttgtgccggg atcttcacgc cgaaatccgg ttccgctttc
     2221 actttcggtg gcgttcctcg ctcaacaggc agtcccgcgt cggaccaggt ttgccagccg
     2281 ccatccagca gacgcacatc tttcacgcct gcgtagagca taatttgcgc cacacgcgct
     2341 ccagcgtata cgtcacgccc gtacagaatg accgtggtgt catggcgaat gccgtgtttt
     2401 gccagcatcg cttttagttg ttcatcagaa actttgttcc acagcggttc gctttccact
     2461 tcgttggtat cgatgtagtc agcgccagga atatggctga taaggtaaag cttaggagcg
     2521 ccctaggccg cttcaatgac tttccagtca ccgacaggtt tcgccgtaac ctctttacct
     2581 tgttgcaggt catgcagcca ttgcggataa accagctgct caaagtgcgg cagtttttgt
     2641 agacgggaag gttcgcttag cgcgtcactc agaatggaga tatgcgtaaa acctgctttt
     2701 tgcagtcgcg ttttgacggc gtcaacatct ttgccattac cgtacagcgc caccggggca
     2761 tcggctttca ggttatgttg cttgatccac gcgttgagct gttcggtgct cattttgtcc
     2821 agccagctag cagagaggtt taaggcggca ggttcatgac cagaagggcc atttaaggtt
     2881 tgtggccagc cgttataaaa cgcgctgggg cgagtatcta tcgctttgcc attttgttgc
     2941 tgaagttggt caagtgtaag aggcttcgcc agttcagcgg cccacgaaga agcgcaagcc
     3001 agccctaaag ccattgccag cgcggtcatt tgagaaacac gtttcatcga aaatcccgac
     3061 attgtagttt tgagtgcgcc attctgaact gacgcattga gaaaaacttt gcgttatcgc
     3121 agtttgttgt aattttctga ccactgcgcc atatccagaa cagaactatc agcaggaaca
     3181 tcctggagat cgtgcgttac ctgaacgacg gggatcgcca gggcgcgaac ttcgctgaac
     3241 acccactggc gaaaattatc gcgcagagcc acatcaagac ggctgaatgg ctcatccagg
     3301 agtaacgctt ttggctgggc gagaagggcg cgtagtagag caacgcgcgc tcgctgaccg
     3361 ccagacaaag tggcaggatc ctgatggaaa gctccgtcaa ggcctgaacg ctcaagtgca
     3421 tcattcacgg cattacgtcg ggcattcccc ttaagtgtcg ccggtagcgc cagcagtaaa
     3481 ttttgcccga cgctgaactg gtcaaataac agtgcatcct gaaaaagaat gccaatctga
     3541 cgctgtgcgg tgggtagcat gtcaatccgt tgctcattga gccatagctc acctgtacaa
     3601 gaaaactgtc cggccagtgc accaatcatc catgaaaaca gagtggattt tccacagcca
     3661 gacggcccca ttaacgtgac gatgtcacct ttatcaaccg taaagttaac gtttgtcagc
     3721 aagcggcttt ctggtaaacg tagcgaaaca tttttcacgc agagcattag cggattcctt
     3781 gtctgacata acctacccat tttgcgacta acgcggtcag ggcaaaaata ataagcggta
     3841 acaacagttg ccataacgcc tgggcggcga gaataccgtt gctgccgccg ctgcttaatg
     3901 ccaccgcctc agtggtgagc gtcggaaaac gccccgcgcc cagccacagc gtcggcatat
     3961 actgcgcaat actgactgaa aatcccaccg cgaaggcaat cagcacaggg cgcaacatga
     4021 gtgggcattt cacgtaaaag aagattttgg cccgcgacca gcccagtgtt tgcgcaatca
     4081 aaattaaccg tgaatcaatg cgctgccagg cgggttgcag gataaacagc atccacggca
     4141 ttacccacag cagatgcccc cagaccaccg ccgtccagct tccatccagg ttcagccata
     4201 gcgccagcgt gtactggccc gccaccagtg gcagagcggg caatagaatg ggcaaccata
     4261 gccacaactg gcgacgctgt gccccccatt ccagccacag cagtaacagg agcaaagcga
     4321 tgaatgttgc taccagcccc attgtcagac tgttgatgag tgcttcacta ttgatcgtcg
     4381 actgatccgc gaggatcgcc agcagaacca cacacagcac accggttaag ggtaaaaaac
     4441 tcgccagcgt agtgcctggt aataaaggtg tggcaggctt gcgaatgcca tctacgcggg
     4501 gaatagtgcg ccgccagctg cgccaaaacg taggcggcga gtaacagcat cagcagcaga
     4561 ctggcgagtg cgcctttcgt ttgttgatcc gcgtcgccct gagttaacca ctgccagcta
     4621 attactgcca gcgtcggtgg attacctggc ccaagaataa tcgccacatc cacgaccgaa
     4681 agcgaccagg caacaatcgc cagcatcgcc attgccagcg gctgtagccc agcgaatcca
     4741 gcacaatgac ctgctgcaac agccgttttt cgctcaatac tgccactaag atccacagca
     4801 gaaatgcgct ttctttcact gcaagggtga ggcccagacc gatccaaatc ggtccattgg
     4861 cggagtgaaa tacgggaaat agtcatatag cagccctccg tcagcaaaga gcagcagggc
     4921 gctggtggca aaagccacat ggggaatggc gagcagccac ggcagacggg cgcacatacg
     4981 ctgccatttc ggcccaggcc atagcgccac aatcaccagc agggcaatca acaatgcccc
     5041 gaccgccgcg atggttgtcg acaccagcgt tgccagtaat gcctgcggta actgtggatc
     5101 ggcaaataac gcctgccagc gtgtcaacga caacgcaggc gatatcagcg tgagagctgc
     5161 cgggatcagc ggtgcataaa tcaccgccac catcgcccac agcaaaaaga ttaatgcgta
     5221 ccgtaacggc gtaaccattc ttgttccagt gcattcaccc atcctgcgtg cggttcagcc
     5281 agtaccggcg gcagatcctg cggcattctt gattgcaata tttcgcgctg cccgtcaggc
     5341 agtttttgcg gatcgagaac agaaggatcg ccccagacaa cgggatctgc tttacgcagt
     5401 tgcgcatcgg gtgagagcag gaaattggca actaccttcg ccgcagcact ggcattcgcg
     5461 ttggctggaa tggtgacgaa atgcacgttg ccaatcatcc cctcgcgaaa gccaaaactg
     5521 taactgcttg ccggcaaatc gccgctggcg attttttgct gcgcatgtgc ggggttaaag
     5581 gtcagcgaca ggcgcaatgt gccggctttc agcagagcat ccatccgcgc gggcgaaggc
     5641 gggaaatcct ttccttcgcg ccacaaatac ggatgcagtg catcaagata ttgctacaag
     5701 ggcgcagtga cacgggcgaa agtcgcatcg tccggcgctt cttttaatgc ggcgggatct
     5761 ggcgtcagca taatcagcaa ctgttcaaga aacgccgtgc cggtaaagtc cggtgggcgc
     5821 ggataagtaa ccgtgccggg attggcttta gcaaactcca gtaaggcttg cggcgtttgt
     5881 ggtggctgtg gcgtaacatc gcggcgggcg ataaacgtca gttgtgcgcc gccccagggc
     5941 gattccgcac cttgtgtcgg cactgaaaaa tctccccgca ccggcagctg tgtgtcgaca
     6001 tagcgccagt tgggcagagt ctccgcccag cccgtttgta ataaattggc ctctttta
//
LOCUS       DAJTSA010000168         6052 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000168 DAJTSA010000000
VERSION     DAJTSA010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.169"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            430..1338
                     /locus_tag="OO369_003901"
     CDS             430..1338
                     /locus_tag="OO369_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR5983982.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="OO369_003902"
     CDS             complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="OO369_003902"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5983983.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3653..4411
                     /gene="loiP"
                     /locus_tag="OO369_003903"
                     /gene_synonym="yggG"
     CDS             3653..4411
                     /gene="loiP"
                     /locus_tag="OO369_003903"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR5983984.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4617..5537)
                     /gene="speB"
                     /locus_tag="OO369_003904"
     CDS             complement(4617..5537)
                     /gene="speB"
                     /locus_tag="OO369_003904"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR5983985.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5673..>6052)
                     /locus_tag="OO369_003905"
     CDS             complement(5673..>6052)
                     /locus_tag="OO369_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5983986.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1493 a         1576 c         1559 g         1424 t
ORIGIN      
        1 ctgaatgtat aactgagatt gatgattctg gaaaataatc actaagccgc ataaattatc
       61 cttattttat ttttagggta ccgtccggcg gtaaaaataa aatactcgtg cggtttaatg
      121 aaagcaattg ggtacgcgag aagaaaaacc ccacaaacca aaataataaa accttaaata
      181 acaacaatat gaaattgata tttttaaagt caactttgta tttgttatgt tcatcacaaa
      241 atcaacattt tgtttgttat tgtatttgtg atgcatatct ctatttattt attgaatgtt
      301 cgaaaacata cttttctaat gcaatgtttt ttcgctatac ccctttatag attctgaatt
      361 ctcacctgtc ggctgttttc tcctcatctc atgtgacata ttcccatact ttactcaagg
      421 aagattgtca tgaaagcacc tgtttcatta acctcatttc gcccacaaaa atctcttgct
      481 atcgccattg gcgtgcttgc cgtggttgtg ctgccatttc tctcgtatta caccgttaat
      541 gaaggtgaac gcggcatttt gctgcgctac ggaaaaatcg taaaagttgc cgaaccgggg
      601 ctgggtttta agatcccttt tatggaaagc gtggagaaaa tttccacgcg taatcaggct
      661 gtggtctatc agggattaca ggcctacagc cgcgaccagc aaccggcaca aatgacagta
      721 tcagtcagct tccacattaa accttcagaa gcaggcgcgg tttataccac ctacaacact
      781 attgaagcat taaaagatcg tctaattgtc cgtcagttgc caactcagct ggagaacatt
      841 ttcggccaat ataccgccat cagcgccgta caggatcgta caaaattggt tcaggatctg
      901 caaaatgcca tgcgcaaagc cgttgttggg ccagtggtaa ttgacggggt ccagattgaa
      961 aatattgatt tttctgacgc ttatgaaaag tcgattgaaa accgaatgaa agcagaagtc
     1021 gccattgcca cgcgtaagca aaatctggaa acagagaaaa tccaggcgca aatagcggta
     1081 actcaggccc aggcagaagc agacagtaaa ctggcggcag ccaaagcaga agcggaaact
     1141 attcgcgtaa gaggcgcggc agaggctgaa actattcgtt taaaaagtgc ggctgaagca
     1201 gaagcaatcc gcctgcgcgg tgaagctctg cgggataatc ccggacttgt agccctcacc
     1261 actgccgaac gttgggacgg taaactgccc gataccatga tccccggcag cacagttccg
     1321 tttatctcta ctaagtaaaa aaagggccgc tttcgcggtc cttctcacaa aacgtcttac
     1381 tgattacagc agcgcttttg ctttcgcaac aacgttatca acagtgaagc cgaactcttc
     1441 aaacagcagc tctgccggag cagattcacc gaaggtggtc atgccgacga tagcgccgtt
     1501 caggccaaca tacttgtacc agtagtcagc aatacccgct tctaccgcaa cgcgtgcagt
     1561 aaccgctttc ggcagtacgg attcacggta agccgcatcc tgcttgtcaa atgcgtcggt
     1621 agacggcatg gacaccacgc gcgctttcac gccttcggca gtcagttttt cgtaggcagc
     1681 aaccgccagt tcaacttcag aaccggtggc gatgaagatc agttccggct gaccagcaca
     1741 gtctttcagc acataaccac cgcgcgcgat gtttgccagc tgctcttcag tacgttcctg
     1801 ctgcgccagg ttctgacggg agaggataag cgcagtcggg ccgtcctgac gctcaacgcc
     1861 gtatttccac gcgaccgcgg attcaacctg gtcacacgga cgccatgtag acatgttcgg
     1921 ggtcacgcgc agagaagcga cctgctctac cggctggtga gtcgggccat cttcgcccag
     1981 accgatggag tcgtgggtgt aaaccatcac ctggcgctgt ttcatcagcg cagccatacg
     2041 tacggcgtta cgtgcgtatt ccacgaacat caggaaggtg gaggtgtacg gcaggaagcc
     2101 accgtgcagg gagataccgt tagcaatcgc ggtcataccg aactcgcgaa caccgtagtg
     2161 gatgtagtta cctgcagcat cttcgttgat tgctttagaa ccagaccaca gggtcaggtt
     2221 agacggtgcc aggtcagcag agccgccgag gaattcaggc aacagcgggc cgaacgcttc
     2281 gatagcattc tgcgacgctt tacggctggc gattttcgcc ggattagcct gcagtttagc
     2341 gataaactct ttcgctttgg cgtcgaagtc agacggcatt tcgcctttca tacggcgggt
     2401 aaattcagcc gcttcctgcg gataagcttt cgcgtaagcc gcaaacttct cattccatgc
     2461 agattctttc gcctggcctg cttctttcgc atcccactga gcatagattt cagacgggat
     2521 ttcgaacggt gcatatttcc agcccagctg ttcgcgggtc agggcaattt cagcgtcgcc
     2581 cagcggcgca ccgtgggagt cgtgagtacc ggctttgttc ggggaaccga aaccgatgat
     2641 ggttttgcac atcagcaggg acggtttgtc agtcactgcg cgcgcttctt caactgcgcg
     2701 tttgatggat gccgcgtcat gaccgtcgat gtcgcgaata acgtgccagc cgtaagcttc
     2761 gaaacgcatt gctgtgtcgt cggtgaacca gccttcaacg tgaccatcga tagaaatacc
     2821 gttgtcatcg tagaacgcaa tcagtttacc cagcttcagc gtacccgcca gagagcaaac
     2881 ttcgtgggag atgccttcca tcatgcagcc gtcgcccatg aaggcgtagg tgtagtggtc
     2941 gacaatgtcg tggcccggac ggttaaactg cgccgccagc gttttttctg caatcgccat
     3001 accgactgcg ttggcaatac cctgacccag cggaccggtg gtggtttcca caccagcggt
     3061 ataacctact tccggatggc ccggagtttt agagtgcagc tgacggaagt ttttcagttc
     3121 ttccatcggc agatcgtaac cggtgaggtg cagcaggctg tagatcagca tggagccgtg
     3181 gccgttggac agcacgaagc ggtcacggtc agcccaggac ggattctgcg ggttgtgttt
     3241 caggaaatca cgccacagga cttcggcaat gtcagccata cccatagggg cacccgggtg
     3301 accggatttg gctttctgta ctgcgtccat gctcagcgca cgaatagcat tggcaagctc
     3361 tttacgtgag gacattttga ctccagatcg gatgatgaag ggcacgccct taacgacttg
     3421 acgacagcgc gttttgggct acgccggaaa atttgccaac aatttaccgc aagccgcgcg
     3481 tcatgtacat ggaacatcct tttgccgctt cagaaatctc tggatcatgc tcgcatgttg
     3541 cgcaatctgc tcgcccgtcc gctgcgcttt tccttatact gagactgagc gtcgattcac
     3601 ctgcaaacgg cgcattttta gaataatcct gaccttgtgc ggaagagaaa acatgaaaat
     3661 tcgcgcctta ttggtagcaa tgagcgtggc aacggtactg accggttgcc agaatatgga
     3721 ctccaacgga ctgctctcat caggagcgga agcttttcag gcttacagtt tgagtgatgc
     3781 gcaggtgaaa accctgagcg atcaggcatg tcaggagatg gacagcaagg cgacgattgc
     3841 gccagccaat agcgaatacg ctaaacgtct gacaactatt gccaatgcgc taggcaacaa
     3901 tatcaacggt cagccggtga attacaaagt gtatatggcg aaggatgtga acgcctttgc
     3961 aatggctaac ggctgtatcc gcgtctatag cgggctgatg gatatgatga cagataacga
     4021 agtcgaagcg gtgatcggtc acgagatggg gcacgtggcg ttaggccatg tgaagaaagg
     4081 gatgcaggtg gcgcttggta caaatgccgt gcgagtagct gcggcctctg cgggcgggat
     4141 tgtcggcagt ttatctcaat cacaacttgg tgatctgggc gagaaattag tcaattcgca
     4201 attctcccag cgccaagaag cagaagccga tgattattct tacgatcttc tgcaccaacg
     4261 cggcatcagc ccggcaggtc ttgccaccag ctttgaaaaa ctggcgaagc tggaagaagg
     4321 tcgtcaaagc tcaatgtttg acgaccatcc tgcatccgcc gaacgtgccc agcatattcg
     4381 cgatcgcatc agcgcggatg ggattaagta aagcctagtg gtgtcgaacg taggtcagat
     4441 aaggcgttca cgccgcatct gacattaatg gcacatttgt taccttgtgc gcattgccgg
     4501 atgcgatgct ggcgcatctt atccggccta cgggtgccga acataggtcg gataaggcgt
     4561 tcacgccgca tccgacatta atggcacgtt ttacccgtgc gcatcgcatc tggtgcttac
     4621 tcgccctttt tcgccgcctg aatatacagc atctccagcg ccagcgtcgc cgcagccaga
     4681 gcggtgattt ccgactgatc gtatgccgga gccacttcca ctacgtccat cccaacgatg
     4741 ttgagatctt tcaggccgcg taccagttta atggcgcgat cggaggtcag gccgccaatc
     4801 actggcgtac cggttcccgg cgcgaaagca ggatccaggc agtcgatatc aaaagtcagg
     4861 taaaccggca tatcacccac aatctgtttc acctgggcga taacgtcatc cacgctgcga
     4921 tcgttcacct ggcaggcgtc cagcacggta aagccgttgt ctttatcaaa ctcggtacga
     4981 ataccaatct gcacggaatg attcgggtcg atcagacctt ctttcggcgc ggtatagaac
     5041 atagtgccgt ggtcaaattc acaaccgttc gcataggtat cggtgtgggc gtcaaagtgt
     5101 accagcgcca ttttgccgaa atgcttcgca tgagcacgca gcagcggcag cgtaacaaag
     5161 tggtcaccac cgaaggagag catacgctta ccggcagcca gcagcttctc ggcgtgcgcc
     5221 tgcagctttt cgctcatctc acgggcatcg ccaaaggcat ataccagatc gccgcagtcc
     5281 acgacgttca gacgctcacg catgtcgaaa ttccacggga agcggttgtg ttcccaggcc
     5341 agattcgtcg aaacctgacg gatcgctgcc ggaccgtggc gaccacccgc acgaccagaa
     5401 gtggccatat cgaacggcac gccagtaatc acccagtctg catcgctgtc atacggctgg
     5461 aagttcatcg gcaggcgtaa aaaaccaaag gcattggaaa ccagtgagtt atcgtattga
     5521 tgacctaagg tgctcatgga tggactccta ttacaaagtc gatataaaaa aacccttccg
     5581 cgtcgttagg cccgacgagg aagggttgga tttgcgaaac acaagtttgt gctaattatc
     5641 gccttaaatg ggcgaggcat caagcgaggt tgctatttcc gacacggtgg cggcgcatag
     5701 ccattgttat ctgccggaaa ggcttgtcca gagcacggcg gcagcgcgtt ttgcgccggg
     5761 ccttgtaatt caaaggcggc aaagccgatt accccggtat tacgcacatc gccttgcgcc
     5821 gaattggctg cgtaggaatc agcaacgttg gcgaactgaa aagccgcttc agtatgttta
     5881 tctttgcgga atcctttaat cactaacgaa tctccggcat tcacaatata gccgttgtta
     5941 ttcagcgaac cttgcttgcc gttcagcaca tccaggccat cagccgtagc aacgatctcg
     6001 tagttggtgt tacgactgta attacggaca tataattgat aattcattcc ga
//
LOCUS       DAJTSA010000169         6009 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000169 DAJTSA010000000
VERSION     DAJTSA010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6009
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.170"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            63..581
                     /gene="mddA"
                     /locus_tag="OO369_003906"
     CDS             63..581
                     /gene="mddA"
                     /locus_tag="OO369_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR5983987.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            578..1027
                     /locus_tag="OO369_003907"
     CDS             578..1027
                     /locus_tag="OO369_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5983988.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1028..1261)
                     /locus_tag="OO369_003908"
     CDS             complement(1028..1261)
                     /locus_tag="OO369_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR5983989.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1347..1520)
                     /gene="ortT"
                     /locus_tag="OO369_003909"
     CDS             complement(1347..1520)
                     /gene="ortT"
                     /locus_tag="OO369_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR5983990.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1715..1810
                     /gene="yncL"
                     /locus_tag="OO369_003910"
     CDS             1715..1810
                     /gene="yncL"
                     /locus_tag="OO369_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR5983991.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2212..3021)
                     /locus_tag="OO369_003911"
     CDS             complement(2212..3021)
                     /locus_tag="OO369_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCR5983992.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3231..4655)
                     /gene="patD"
                     /locus_tag="OO369_003912"
     CDS             complement(3231..4655)
                     /gene="patD"
                     /locus_tag="OO369_003912"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCR5983993.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4677..5471)
                     /locus_tag="OO369_003913"
     CDS             complement(4677..5471)
                     /locus_tag="OO369_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5983994.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5461..5808)
                     /locus_tag="OO369_003914"
                     /pseudo
     CDS             complement(5461..5808)
                     /locus_tag="OO369_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT         1565 a         1528 c         1342 g         1574 t
ORIGIN      
        1 tgcttcgggc tgttttcgat agtattgata acatcatcat cattatttta ttgaggccag
       61 acatgtccat ccgttttgcc cgcaaagccg actgtgctgc cattgcggaa atttataacc
      121 acgccgtgtt gtatacggcg gctatctgga atgaccaaac ggtggatgca gataaccgca
      181 ttgcctggtt tgaagcgcgg accatagcag gttatccggt gctggtaagt gaagaagatg
      241 gcgtagttac gggatatgcc tcgtttggcg actggcgtag tttcgatggt tttcgccata
      301 ccgtggaaca ttcggtttat gtccatcccg atcatcaggg caaaggtctg gggcgtaaat
      361 tgttaagtcg attgattgat gaagcgcggg attgcgggaa gcatgtcatg gtcgccggga
      421 tcgaatcgca aaatcaggct tcgctgcatc tgcaccagtc gctgggattt gtcgtcaccg
      481 cacaaatgcc gcaggtaggc actaaatttg gtcattggct ggatctgaca tttatgcagt
      541 tgcaactcga cgagcgcact gaaccggacg cgattggatg aatcagtcgc tcacccttgc
      601 ttttttgatt gccgccggaa ttggtctggt agtccagaac acgctgatgg cgcgcatcac
      661 ccagacctcc tctaccattc tcatcgccat gttgctgaac tcactggtgg ggattgtgct
      721 ctttgtttct attttatggt ttaaacaagg catggcgggg tttggcgaac tggtgtccag
      781 cgtgcgctgg tggacactta tacctggctt gctgggttcg ttttttgtct tcgccagtat
      841 cagcggatat cagaatgtgg gagcggcgac aactattgcg gtactggtcg ccagccagct
      901 gattggcggt ctggtgctgg atatcttccg cagtcatggc gtaccactac gggcgttgtt
      961 cggcccaata tgtggcgcaa ttttacttgt ggttggcgcc tggttggtgg ccagacgctc
     1021 attttaatca gaggatggtg ccgcctttgg ttaactgttc gtggcgcgct tccatatctt
     1081 ctttatgctt gcgaccgtga tgagcaatag cggtgcgcaa acgctgctgc tgggtgtagc
     1141 gatcttcccg acttaactcc gcgtcatcgc tcagggcgat taataattcg ttcatatggg
     1201 caatgacgct ctcttcaatc gcggcatcca cgcgcgcgat gacttcatcc agatgcgaca
     1261 tactttctcc ttagccactt gtgaaattta tcgattaatc caaatgatat aagtctgcgt
     1321 ggtgccgtca agccagtgag taagatttaa aacagtgaaa acaacagcgc caccgggaaa
     1381 ctcatcggcc atgtggcccc caccagaaag gcgcttaaga aacggatgcg tttcttatcg
     1441 tgagaaagaa accaggtgat aagaaatgcg attgctgcca taaccgcata aaaaacaagc
     1501 atatgttgat agagaaacat gtacgggcgt ccagccatat gaataaaacg cgcgcaaata
     1561 tgcgccattt tttgacgtgc atcaagtttt ttcaccagtt tgagaaaaaa tcatcaaaca
     1621 gcaacttgcc cttatccatc ttcagaatga gtattattgc ggattttctt aacccgtact
     1681 atacacatag gggcaatgat aaaaggtggc aaaaatgaat gtttccagta gaactgtagt
     1741 actgataaat gtctttgctg ctgttggttt gtttactctt atctctatga gatttggctg
     1801 gtttatttga tgtagtgatt ttgcacgcag cacgtactgt cagagaaatc aactctctga
     1861 cagtacgcaa gatcttctca tactctccaa gtttgcaaca ttatccttaa tatttacttc
     1921 tttttgctgg catctacatt gaaatgagta gaaaacatcc ccctactgtc gtctatacaa
     1981 acagcttact gattcaaacg gaatcttgtt ttcacatttt tctatcacga atactttata
     2041 cgcgcgggag tctataaatg ctttatactt tcaaggaata acgtaaatat aaaaagatgg
     2101 cgtaaagtat atgcatggca ggatgtttgc aacctccttg atgacgctgc cccattgaaa
     2161 ttgcaaaccc cgccctggtt atcttgccca tttatcatgc aaaacagata attataggcc
     2221 tctgaatacc tcagaaagat tgaatttttt atagtccctc tcttgaatat ttccatttga
     2281 tatcaatcga ttcatctctt caacaaaata atttatatca tcacacttat tattatccgg
     2341 aagatctgag tagttataat tccaccattt tattctcaac agttcatcaa taacatttga
     2401 atcaaaccta aatctgatta tttttgcagg aactcctgca actatggcat atgggggcac
     2461 atctttggta acaacggaat tggcagcaat aacagcacca tcacctattg cgatgccacc
     2521 tttcaataca acatcatttc ctatccagac atcatttcct ataatcggat tttcaggttt
     2581 ggtaatgaat ggtttcaatg tccagctacg ttcaaattca ctcatagcaa acttatcaaa
     2641 ttcaccgtta taagtcatca tgtgagtagt aaaacgattc aaaggatgat ttccacccat
     2701 tatttttaca tttgatgcaa tactacaaaa tcgacctata cgtatattcc ctgaaaaatg
     2761 gcaggtcgga aatgaaaaag cgccgacact ggagaaattt ttcacaggca ttgttgaata
     2821 ttcttcagca ataacattat cttcaattgt tatcttgtca ccttttgaga aaagaaataa
     2881 attcctttct ttatacgact tcttcccctt atgatttaaa aagattttat tatccataca
     2941 aaactcaaag tgttttttcg tccatctgaa ggtaagggtt ttatttttat cttccgtgaa
     3001 tttgtacaat tgaactttca ttgttaatac ttctacttat aaagtattag tttaataacg
     3061 atactctaaa gccttatgct aatcaactgt gacaggatga aaaatcgcca tcaaacattt
     3121 agcctaattt ttacgccagt tattaatgta atcattctaa tacttaaccg ccattaaact
     3181 cagaacctaa aacgatagtg gccgtcctat aacagccact aacatctaac ttaatgttta
     3241 accatgacgt ggcggacgac ggtgtaatcc tccagcccat aaagtgacat atccttgccg
     3301 taaccagaaa gtttctgccc accgtgcggc atttcactta ccagcatgaa atgggtattg
     3361 acccaggtac aaccatattg cagccgtgcg ctgacgcgat gcgccctgcc cacatctttc
     3421 gtccataccg aagatgcaag tccgtactgg ctgtcattcg cccagttcac cacctgttct
     3481 tcgttgtcga agggcgtaac actcactact ggaccaaata cctctttttg cacgatggca
     3541 tcgtcctgta atgcgccagc cagcagcgtc ggcgcatagt aatagccatt acccttgcgc
     3601 ttttcaccgc cagtgatcac tttgatgtgc cctgtcgctt tcgcctcttc tactgccttg
     3661 ctgacgcgtt cgagatgcgc cagcgagctt aaaggtccaa gctccgtaga ctcgtcatcc
     3721 ggcgcaccag attttaacgt tgccaccgca gcacccagtt tttccaccag cgtatcgtaa
     3781 atgccttttt gcgcgtagat ccgacaagcc gcagtacaat cctgtccagc attgtaatag
     3841 ccaaatgtac gtacaccttc gaccactgct tcaatatccg catcatcaaa aacaatcact
     3901 ggcgctttgc caccaagttc catatgagta cgcttaatgg acgacgcggt atggctgatg
     3961 atgtgctcgc cggtggcgat agagcccgtc agcgacacca tccgcacttt gggatgaccg
     4021 gtcagcggat cacccaccgt tttgcctctg ccaaacagta tgttaatcac gcctgccggg
     4081 aagatatctt tcgccagctc tgccaacttc aacgcggtca gcggggtaat ttctgatggt
     4141 ttaagcacta cgcagttccc tgccgccagc gccggagcaa gtttccacgc ggccatcatc
     4201 agcggataat tccacggtgc gatagaagcc acgaccccca acggatcgcg acggatcatc
     4261 gaagtatgac cttcaagata ttcacctgcc gccagaccat tcagacagcg cgccgcaccc
     4321 gcgaaaaagc gaaaaacatc gacaatcgcc gggatttcat cattgaacgc actatgcagc
     4381 ggtttgccac aattacggga ttccagttcg gcaaaaacct gaccattttc ttcgataaca
     4441 tccgccagtt tcagcaggca ttccgcacgc gctttcggtg tggtttgccc ccattcagca
     4501 aatgctgcat ctgccgcacg cacagcagca tcgacctgct ctgcggatac ctcggcaatc
     4561 tccagtaaaa cgtcccccgt tgccggatta tagacaggct gtttttcccc ttcgccgcta
     4621 acccgttctc cgttaatcag taacttatgt tgcatagctt attcctgtat cagtggttat
     4681 tgaccattgt cgccttcgcg ggttagccac caggccccca ggatcggcaa ggttgttacc
     4741 aacataacca gcagtgccac cacgttggtt accggtacat cacgcggtcg cccaagctga
     4801 ttgagcaacc acaacggtaa cgttcgttca tgacctgccg taaaggtcgt aacgatgatt
     4861 tcatcgaacg acaaggcaaa cgccagcatt cctcctgcca gtaacgccga actgagattc
     4921 ggcaacacta cgtagcggaa ggtttgccag ccattggccc caagatccat tgacgcctca
     4981 accagactcc acgaggtgcg gcgaaaacgg gcgatgacat tgttaaacac caccactacg
     5041 caaaaagtcg catgaccgac cacgatggtg aaaaatcccg gctccagatt gatggtttta
     5101 aaggcggtta atagcgccag accagtgaca atgcccggca gcgcaatggg cagcagcaat
     5161 aacagcgaaa tggcgttttt gccgaaaaag tctcgtctcc acagcgcggc agctgccagc
     5221 gtccctaaca ccagcgcaat taatgtcgcc agcgccgcca ctttaagtga cagcgtcacg
     5281 gcatcaagaa tatcactacg ctgtgctgcc acgctaaacc agcgcagcgt caggccctgc
     5341 ggtggaaaac taaacgccgc atcttcagtg ttaaacgcat aggcggcgat tatcaggatg
     5401 ggaaaatgta ggaaaacaac gccgccccag gccgccagtt tgaggaaaaa cggtgcgcgt
     5461 tcagagtgca tcgaacgctc ccagacgttt cacgaacgcc aggtacagtg cgatgagaat
     5521 aatcggcacc agggtgaatg ccgccgccat cggcatattg ccaatcgccc cctgctggga
     5581 ataaaccata ttgccgataa aatatcctgg aggaccaacc agctgcggga cgataaaatc
     5641 gcccagtgtg agtgagaagg taaagataga gccagcggca atacccggga ttgccagcgg
     5701 cagcaccaca tagcgaaagg tttgtcgtgg acgtgcgccg agatcagccg acgcctgcaa
     5761 caatgacggc ggcaaacgct caagcgccgc ctgaacgggc aggatcatcg agtaggcagc
     5821 ctgacggctg cggcttgtca tggtctgcaa ttaccgttat aaaaacaggc aatgtcattg
     5881 tctttcaggt ggttatatgg cccgttcagc taaaccccgt aaacgtaaac ccgcctctca
     5941 aagttcctga gtcgtgaccc gttcgaatgc gtgctgtgtg gcggccggat ggtataccgc
     6001 cgggccatt
//
LOCUS       DAJTSA010000170         5982 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000170 DAJTSA010000000
VERSION     DAJTSA010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.171"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..392)
                     /locus_tag="OO369_003915"
                     /pseudo
     CDS             complement(1..392)
                     /locus_tag="OO369_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(578..3886)
                     /gene="icsA"
                     /locus_tag="OO369_003916"
                     /gene_synonym="virG"
     CDS             complement(578..3886)
                     /gene="icsA"
                     /locus_tag="OO369_003916"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR5983995.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4415..5617
                     /gene="virA"
                     /locus_tag="OO369_003917"
     CDS             4415..5617
                     /gene="virA"
                     /locus_tag="OO369_003917"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR5983996.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5796..>5982
                     /locus_tag="OO369_003918"
     CDS             5796..>5982
                     /locus_tag="OO369_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5983997.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT         1840 a         1355 c         1018 g         1769 t
ORIGIN      
        1 caggctgtac gcttccccat gtgatttgcc tcgccacttc accgggctca ggaccccgta
       61 ggatcttcac cgaaaaaaat gcatcaccag ttgctttatt atttgtttta ggtagacctg
      121 ttactaattt atacctactc tgaccaagtc tggataagaa aggcacacct gcacatttct
      181 acttggaaat gttttctttt accattttgc tcaaaaattt caacatccaa tattcctcgt
      241 acagaactac tcaagtcctg aatagaaaaa gttccagctg gtacagtaga gtcatatatt
      301 actcgacctt gttgagatac aacaacacgt gcattagttt ctgcaatacc agtaatctga
      361 ggcgcataac cacgaatatt tggcggtagc attcgttcat cgctttctaa tgcaattcct
      421 gtgtaacgcc aagcaacagc gcattctggc gcatgatctg gtaaacacgt ttggcattga
      481 tcgcaggcat accatcatgt gcacaaaaac ccaccgagct tttacacggt gggtcccaga
      541 gaaatgcagg acatcaacac gccctgcatt tttattatca gaaggtatat ttcacaccca
      601 aaataccttg ggtgtctctg taactgttat tacccaattg ctgagatacg tttccccaga
      661 tgcttaggtt atctgcaaga cgccccccca caccagtttt gagttcaatc acattacggt
      721 tgcctatctg gtgatttgac atgccattca taattacacc atattgcttt gagctccatt
      781 tccaattcac ttccaaaaat ggttcgaact tacgaagagt ctccttatcg atacaactct
      841 gactaattaa ggatgtacgc aatcccaatt ttgcttgaat attattgccc ccagaggatg
      901 tgaccatagt gccattgtgt tcaatatagt cgtttgcttt taccccatgc ctagttatct
      961 ggacttgtgg attgagatac aatgcgttgt caatattatt atgagcagtc cagcgtatgg
     1021 ttgggtagat atagccagct tccaaagctc ctgttattcc tttgctggaa tatttctcac
     1081 cagttaaccc atctcctttg actgtattat taaaccaact gtactgcaac caagcatcca
     1141 tatatagacc tgttcgttct ttttcatcct gaaaccaaga actgtacaaa ccggcagtat
     1201 aaccgctcac agttcctcgt gaactataac tagtatactt attatgagtt ttgctatcct
     1261 gatatccata agctcccata atacccacat gcaagccatc atgatcagtg aagtttgtct
     1321 tcaaaatatc tccaccaatc tgataaacca tcctgttagc tgtggttttt aattgtctat
     1381 cagctaaatt atgctcatta tgtccaccaa cagtacgaat ccacatggtg gtttcgctga
     1441 gctgacctgt agtaggatca acaatagtgc taccgtgtcg gtcatagaga gaaggactaa
     1501 acatcgtgtt cattgctatc aggttagcaa tataactgcc agcttcaggg cgccatgtgt
     1561 gaatactacc ttcacccaaa ttaagtgaag atacaagctg tgaactagct cggcgactac
     1621 tcatttgagt agactcttga ttgctcatct gttttgattc ttgattatcc atctgactag
     1681 ttagatacca cttattggta ttcagtccag atacagtgcc ctgtttcagg cgatagtcat
     1741 aactaccagc aacaatacgg cctttctgaa taaaagcatt cttatcagaa gaatctgtcg
     1801 aaataatttg aacaccttca agagttttat ctccagagcc tccctcattt gtcactgcaa
     1861 cataggtaat accagacgca tggcctgcaa tttccagccg gtcagtttta ctgttgtcat
     1921 tgccgagaac cgcgcccaaa gataatattc cgccctttcc atgccaatta cctttctgaa
     1981 cataggtctg accgacattt gacgaactat tattgagaat aacatgaccg tttttctcca
     2041 gagtcatgtt acctgtgaca atgacggggt caggcaaaaa aggggcatta atattattga
     2101 ttagcacagt cccactattt aataaagtgt taacagtttg gttcatgcct gaaaaattca
     2161 aggtagcacc tttattaaca ataacaccag cggtacgtgt catagcttca actgtcccca
     2221 tttttagaat gcctgcattg atgttggtga aaccagagta ggtatttttt tccgccaaaa
     2281 tgagagttcc ctcccctgat tttgttaggg attttccgtc ccagctattt atattatgat
     2341 ggctgagatt atctgccaga atagtactaa cagtaagttc agcgttttct ttcagattga
     2401 actctccatg actgtcgcca tctgtatcat tccagctcaa accgtagatc aatttattct
     2461 ggtcactgat atagcttttt gtatagtcaa tagcaccagt agtgagtaac ggattgatga
     2521 tagatatatt agaaacactg aaacctgtta tccctttatc tgcacttagc agtttatagt
     2581 ccttctgcgc ccagttattt ataatagcct ccacttttac attgttgcca aatgacatat
     2641 ttccagaagc ttccagacta ctgattccag ctaaatttaa agaactgttc tcaccaaaag
     2701 aaacgtcacc tgaaacagtg aatttgttac ctgatagtga aacagttgta caatcaccag
     2761 cacataaatc accttcaata gttggtgtgg tcccagtgta atcattgttg ataattttca
     2821 gaatggatga attgttaacc tgtacatcac cagtaatgac agaaccttca tggagtatta
     2881 atgaattgtt tccaccagtg atgtggatag cattacctgc tacattttgt gttccatcat
     2941 cttctttacc tgaaattata tatccgctat tagcaatggt catattgcta ccatttatag
     3001 catcccccca atgtccacca ttaccgccta aaatagctcc attgttgatt acagacaggt
     3061 tatctcctgt gatagcatta ccaccgtagc catcataacc ataaccgtaa gcagcacctc
     3121 cgttgcctcc agcgaacgtg cccttattga ttacagagag gctgcttcct gtgatggcat
     3181 cacctccatt accgccatca ccctcaccat aattgttacc tccattacca ccggaaatag
     3241 tccctttgtt gattatgggt aaattgactc ctgtgacggc atcacctcca ttccccccat
     3301 cactatcact ataactatcg ccaccatgtc cacctgaaat aatttctcca tttattataa
     3361 acagattgtc tcctgtaaca gcctcaccac catcaccgtt atggtcagca ccgctaccgc
     3421 cgctaccacc aagaatcatg ccttgattga ttatagacaa gtcagatccg gtaatagagt
     3481 caccaccatt accgccacaa ctattaccat cattattatc accagcacca ccatgacctg
     3541 cgctaatcat aatatcagag ttaatccgta gcttagttct agatgcatga gaggggttgg
     3601 aagaagttat ataccacgca tccattccat cttcaccagc tcccaaggga gaaagtccat
     3661 caacaggtgt taataatttt tcataattgt cctctgaaaa atgaagttct tgagtacccg
     3721 aaagaggagt agcaaaagct attggccccc cgagcaacag ggatgcacca acaacaaaag
     3781 gagaaaaaga caatttgcat gttttttcct ttaccgtagg taattctccg gcccccccct
     3841 gtgaacattg ggtcatatta caaaaaaatt tgtgaatttg attcatgcac tatattatca
     3901 gtaagtggtt gataaacccc tgaaaagatt ccgttttatc cggaataaag ggacgatata
     3961 tgcaaaaaca tattaaacaa agcctcaacc aaaacatgag agctgtgtta tatatcaatg
     4021 caacgggaga aatttacctg tgtcttgctt tgaaaaaaag agggggaaag ctatatgttc
     4081 gataataaat taataatttt actatgatag tctgcttcag accgcaactt ttcagttctt
     4141 tctgtacgct tgcccaaagt ttaaccactt tgtttttcta tacacccaat ggttctatct
     4201 gccccttgat gttaatcccc aaagccaata acatgaatgt attcatatca caactttcct
     4261 accagactct ccaatgcaat aactgcataa ttgtttttag tggaaatgta tcgttttctt
     4321 aaagagaaga ataacattcc attttattat gttcccaatt taattatatt aatatatatt
     4381 ggcattaata ggaaaataca tcaggagaaa tcaaatgcag acatcaaaca taactaacca
     4441 tgaaagaaat gactcatcat ggatgtcaac tgtcaaatca actacagaag taagttggaa
     4501 taaattgagt ttttgtgaca ttttactgaa aataataact ttcggcatat attctccaca
     4561 cgaaactcta gccgaaaaac atagcgaaaa aaaactaatg gatagtttct caccatcact
     4621 atcacaagat aaaatggacg gagagtttgc ccacgcaaac atagatggca tttcaatacg
     4681 gctatgcctt aacaaaggca tatgttcggt tttttatctt gatggagata aaatacaatc
     4741 cactcaactt tcatcaaaag aatataataa cttgttaagt tcactccccc ccaaacaatt
     4801 caacttagga aaagtacata ctattacagc tccggtatca gggaatttta aaactcacaa
     4861 gcctgcacca gaagttattg aaacagccat taattgctgt acatcgataa taccaaacga
     4921 tgattacttc cacgttaaag acactgattt taatagtgtt tggcatgata tttacagaga
     4981 tattcgagca tctgacagca atagtacaaa aatatatttc aacaatattg aaataccact
     5041 aaaattaatt gcagatctaa ttaatgagtt aggaattaat gaattcattg atagcaaaaa
     5101 agagcttcaa atgctaagtt acaaccaagt aaataaaata atcaattcaa acttccccca
     5161 acaagaccta tgttttcaaa cagaaaaact tttgtttact tcactctttc aagaccctgc
     5221 atttatatct gcactaacat ctgcattctg gcaatctctt cacatcacgt cttcctctgt
     5281 ggaacacata tatgcccaaa ttatgtccga aaacatagaa aacaggctta atttcatgcc
     5341 tgaacaacga gttattaaca attgtggaca tattataaaa atcaacgcag ttgtccctaa
     5401 aaacgacact gcaatctctg cctctggggg gagggcttac gaagttagct catcaatatt
     5461 accatcgcac ataacatgta atggtgttgg tattaataaa atagaaacaa gctatctcgt
     5521 tcatgctgga acacttccaa gcagtgaagg gcttcgcaat gctatccctc cagaaagtcg
     5581 tcaagtatca tttgccatca tatctcctga tgtttaagcc aatattatat tggctgccag
     5641 actgtaaaaa aacgttcttc agaacagctc tataaactat taaaccaaag ctaaaaggat
     5701 ggggaccaat agaatctcag cccatccttt ttatatcatt atgaaccgcc ccggaaatcc
     5761 tggagactaa actccctgag aaagaggtaa acaggatgac taaaaataca cgtttttccc
     5821 ctgaagtccg tcaacgggca gttcgtatgg ttctggaaag tcagggcgaa tatgactcac
     5881 aatgggcagc gatttgttcc attgccccaa agattggctg tatactggaa actctgcgtg
     5941 tctgggtacg ccagcatgag cgggataccg ggggcggtga gg
//
LOCUS       DAJTSA010000171         5889 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000171 DAJTSA010000000
VERSION     DAJTSA010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.172"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            50..244
                     /locus_tag="OO369_003919"
     CDS             50..244
                     /locus_tag="OO369_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCR5983998.1"
                     /translation="MLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVR
                     EILEEIEASILSESVNAKAG"
     gene            511..1437
                     /gene="tauA"
                     /locus_tag="OO369_003920"
     CDS             511..1437
                     /gene="tauA"
                     /locus_tag="OO369_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5983999.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1450..2217
                     /gene="tauB"
                     /locus_tag="OO369_003921"
     CDS             1450..2217
                     /gene="tauB"
                     /locus_tag="OO369_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR5984000.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            2214..3041
                     /gene="tauC"
                     /locus_tag="OO369_003922"
     CDS             2214..3041
                     /gene="tauC"
                     /locus_tag="OO369_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR5984001.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            3038..3889
                     /gene="tauD"
                     /locus_tag="OO369_003923"
     CDS             3038..3889
                     /gene="tauD"
                     /locus_tag="OO369_003923"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR5984002.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="OO369_003924"
     CDS             complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="OO369_003924"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR5984003.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            5493..5861
                     /locus_tag="OO369_003925"
                     /pseudo
     CDS             5493..5861
                     /locus_tag="OO369_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1399 a         1454 c         1598 g         1438 t
ORIGIN      
        1 agaaatgttg tcgtttctta ccagtgagga acgagaagcc cttacaaaca tgctcgcaag
       61 gaaaggtgtt gagactgtgc tacgcttgtt agataaggaa actcaagagc tactgcaact
      121 agagggccgt aaacgcctgg cagcgttgat gctcgttgat atgcccgacg agcaagtcag
      181 agagattttg gaagagattg aagctagtat tctctcagag agtgtaaacg cgaaggcggg
      241 ttaacttcat tgacctgcct ttagggtgcc tttaaaattc gtttttaaag gctacgatga
      301 catttttgaa gagtcttaaa agaattaaaa tacacggcgc gcctatcttg aatgttttat
      361 cgatatcgaa gcattgagca tttttaactc gttttatctt gaatgcaatc ggcgcataac
      421 ttattgaatc ccatcaaatc ccgtcttttt ttacccattc ccggttatct tgaataaatt
      481 acctagtcat ataacacact tcttgccgca ctggcattca tcgcttttca ggcacaggcg
      541 gtgaacgtca ccgtggcgta tcaaacctca gccgaaccgg cgaaagtggc tcaggccgac
      601 aacacctttg ctaaagagag cggggcgagc gtggactggc gtaagtttga cagcggagcc
      661 agcatcgtgc gggcgctggc ttctggcgac gtgcaaatcg gcaacctcgg ttccagccca
      721 ttagcggttg cagccagcca acaggtgccg attgaagtct tcttgctggc gtcaaaactg
      781 ggtaactccg aagcgctggt ggtaaagaaa actatcagca aacccgaaga tttgattggt
      841 aaacgcattg ccgtaccgtt tatctccacc acccactaca gcctgctggc ggcgctgaaa
      901 cactggggga ttaaaccagg gctagtggag attgtgaacc tgcagccgcc cgcgattatc
      961 gccgcatggc agcggggtga tattgatggt gcttatgtct gggcaccggc ggttaacgcc
     1021 ctggaaaaag acggcaaggt gctgaccgat tctgaacagg tcgggcagtg gggtgcgcca
     1081 acgctggacg tctgggtggt gcgcaaagat tttgccgaga aacatcctga ggtcgtgaaa
     1141 gcgttcgcca aaagcgccat cgatgcacag caaccgtaca ttgctaaccc tgacgcgtgg
     1201 ctgaaacagc cggaaaacat cagcaaactg gcgcgtttaa gcggcgtgcc tgaaggtgac
     1261 gttccggggc tggtgaaggg aaatacctat ctgacgccgc agcaacaaac ggcagaactg
     1321 accggaccgg tgaacaaagc aatcatcgac accgcgcagt ttttgaaaga gcagggcaag
     1381 gtcccggctg tagcgaatga ttacagccag tacgttacct cgcgcttcgt gcaataaaag
     1441 gaggcgcaga tgctgcaaat ctctcatctc tatgccgatt atggcggcaa accggcactg
     1501 gaagatatca acctgacgct ggaaagcggc gagctactgg tggtgctggg gccgtccggc
     1561 tgcggtaaaa ccaccctgct gaatctgatt gccggttttg tgccttatca gcatggcagc
     1621 attcaactgg cgggtaagcg tattgaggga ccgggagcag agcgtggcgt agtttttcag
     1681 aatgaagggc tgctaccgtg gcgcaatgta caggacaacg tggcgttcgg cctgcaattg
     1741 gcaggtatag agaaaatgca gcgactggaa atcgcgcacc agatgctgaa aaaagtgggg
     1801 ctggaaggcg cagaaaaacg ctatatctgg cagctttccg atggtcaacg tcagcgggtg
     1861 gggattgccc gcgcgctggc ggcgaatccc cagctgttat tactcgacga accgtttggt
     1921 gcgctggacg ccttcacccg cgaccagatg caaactctgc tgctgaaact ctggcaggag
     1981 acgggcaagc aggtgctgtt gattacccac gatatagaag aagcggtgtt tatggcgact
     2041 gaactggttc tgctttcatc cggccctggc cgtgtgctgg agcggctgcg gctcaacttt
     2101 gctcgccgct ttgttgcggg agagtcgagc cgcagcatca agtccgatcc acaattcatc
     2161 gccatgcgcg aatatgtttt aagccgcgta tttgagcaac gggaggcgtt ctcatgagtg
     2221 tgctcattaa tgaaaaactg cattcgcggc ggctgaaatg gcgctggccg ctctcgcgtc
     2281 aggtgacctt aagcattggc acgttagcgg ttttactcac tgtatggtgg acggtggcgg
     2341 cgctgcaact gattagcccg ctgtttttgc cgccgccgca acaagtactg gcaaaactgc
     2401 tcaccattgc cggaccgcag ggatttatgg acgccacgct gtggcagcat ctggcagcca
     2461 gcctgacgcg cattgtgctg gcgctactcg cggcggtggt gatcggtatt ccggtcggga
     2521 tcgccatggg gctaagcccg acggtgcgcg gcattctgga tccggtaatc gaactttatc
     2581 gtccggtgcc accgctggct tatttgccgc tgatggtgat ctggtttggt attggtgaaa
     2641 cctcgaagat cttactgatc tatttagcga tttttgcgcc ggtggcgatg tcggcgctgg
     2701 caggggtgaa aagcgcgcag caggttcgca ttcgtgccgc ccagtcgctg ggtgccagcc
     2761 gtgcgcaggt gctgtggttt gtcattttgc ccggcgcgct gccggaaatc ctcaccggat
     2821 tacgcattgg tctgggtgtg ggctggtcta cgctggtagc ggcggagctg attgccgcga
     2881 cacgcggttt aggatttatg gttcagtcag cgggtgaatt tctcgcgact gacgtggtac
     2941 tggcggggat cgcggtgatt gcgattatcg cctttctttt agaactgggt ctgcgcgcgt
     3001 tacagcgccg tctgacgccc tggcatggag aagtacaatg agtgaacgtc tgagcattac
     3061 cccgctgggg ccgtatatcg gcgcacaaat ttcgggtgcc gacctgacgc gcccgttaag
     3121 cgataatcag tttgaacagc tttaccatgc ggtgctgcgc catcaggtgg tatttctgcg
     3181 cgatcaagct attacgccgc agcagcaacg cgcgctggcc cagcgttttg gcgaactgca
     3241 tattcaccct gtttaccctc atgccgaagg ggttgacgag atcatcgtgc tggataccca
     3301 taacgataat ccgccggata acgacaactg gcataccgat gtgacattta ttgaaacgcc
     3361 acccgcaggg gcgattctgg cagctaaaga gttaccttcg accggcggtg atacgctctg
     3421 gaccagcggt attgcggcct atgaggcgct ctctgttccc ttccgccagc tgctgagctg
     3481 gctgcgtgcg gagcatgatt tccgtaaatc attcccggaa tacaaatacc gcaaaaccga
     3541 ggaggaacat caacgctggc gcgaggcggt cgcgaaaaat cctccgttgc tacatccggt
     3601 ggtgcgaacg catccggtga gcggtaaaca ggcgctgttt gtgaatgaag gctttactac
     3661 gcgaattgtc gatgtcagcg agaaagagag cgaagccttg ttaggctttt tattcgccca
     3721 tatcactaaa ccggagtttc aggtgcgttg gcgctggcaa ccgaatgata ttgcgatttg
     3781 ggataaccgc gtgactcagc actatgccaa tgccgattac ctgccacagc ggcggattat
     3841 gcatcgggcg acgatccttg gggataaacc gttttatcgg gcggggtgat acgagtgcgg
     3901 acggttccct cgcccccttg gggagagggt tagggtgagg gggcgttcac tgtactttca
     3961 acaggttaac tccccctttc tgagaggaaa aaaattaacg cagaatcttc ttctcagcca
     4021 aatccagcgc aaagtagctg aaaatcagat ccgcacccgc acgcttaatt gaacctaagc
     4081 tttcgagcac gactttctct tcatctatag cacccgccag cgcggcgaac ttaatcatcg
     4141 cgtactcacc gctcacctga tacgcgccaa tcggcaattc agtacgttca cgcagctcac
     4201 gcacgatgtc gaggtacgct ccagcaggtt taaccatcag gcagtctgcg ccctgggctt
     4261 catccagcag tgactcacga atcgcctcgc gacggttcat tgggttcatc tgatagcttt
     4321 tgcggtcgcc ttttaatgcg cttcctgcag cttcacggaa cgggccataa aaggaagagg
     4381 cgaacttggt cgaatacgac ataatcgccg tatctttaaa gcctgcagcg tccagcgcct
     4441 gacgaatcgc ctgtacctgg ccgtccatcg cggcggaagg ggcgatgaag tctgcgcctg
     4501 cagcagctgc aaccacggct tgcttgccta aattttccag agtcgcgtcg ttgtcgacgc
     4561 catgctcgca cagcacaccg cagtgaccgt gagaagtgta ttcacagaaa caagtgtctg
     4621 acatgacgat catttctggc acagtctgct tgcagatgcg cgacatacgc gccaccagtc
     4681 catcttcccg ccaggcgtcg ctgccggttt catcggtatg gtgagagatg ccaaaagtca
     4741 ttacggaacg gataccggcg ttggcgatgc gttcaatttc gcgtgccaga tgtttctctg
     4801 gaatgcgcat cacgcctggc atggcttcaa cggctttgta gtcgtcaatt tcttcttcaa
     4861 caaagatcgg caacaccagg tcgttaaggc taagtgttgt ctcttcaaac atagcgcgca
     4921 gcgcaggaga tttgcgcagg cgacgagggc gttggattaa gtctgtcatg gtctgcctga
     4981 tgtttgtgga attaaggtgc acagtatacc tgaagcgggg caggagtgtt ttacgaaagt
     5041 tgtcgcgatg ttgacaagaa gagaatgaaa gagagaccag gacatctttg taatagaaat
     5101 agcaataatt tcatattcac tgaaaatatt tttaatcttt atttatagct cttggttatt
     5161 attttttggc gtttggttgt gctgctataa attgaataat tcaaactttg ttgtatattg
     5221 ataagatatt tatctggttt gtatttttga tatttataac gcgtatgcat caaaaataac
     5281 attatctgca acatactgaa atatcgtcct tttatcaatt tgatgcgtgt ttttctccat
     5341 aaattatata tgtccacatt cggacttagg ggaaagaata attgaaccat tcgtctgtaa
     5401 cgcagcataa tcgttagcgc gaaacataat atgttttcta tgcattgata attgatggat
     5461 caacttatta cgtccctgag gagggatgac aaatgcactc ctggaaaaag aaacttgtag
     5521 tatcacaatt agcattggct tgcactctgg ctatcacctc tcaggctaat gcagcgacca
     5581 acgatatttc tggtcaaact tacaatactt tccatcacta caacgacgcc acctatgctg
     5641 atgacgttta ctatgatggt tatgtaggct ggaacaacta tgccgctgat agctattaca
     5701 acggcgatat ctacccggtc attaataacg ctaccgttaa cggcgtgatt tctacctact
     5761 atctggacga cggtatttct accaatacca acgccaatag tctgacaatc aaaaacagca
     5821 ctattcacgg tatgatttat tccgagtgca tgacaactga ttagactggc cccctgaatc
     5881 tccagacaa
//
LOCUS       DAJTSA010000172         5738 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000172 DAJTSA010000000
VERSION     DAJTSA010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.173"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..241
                     /locus_tag="OO369_003926"
     CDS             <1..241
                     /locus_tag="OO369_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984004.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="OO369_003927"
     CDS             complement(238..1179)
                     /locus_tag="OO369_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCR5984005.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="OO369_003928"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="OO369_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..2904)
                     /locus_tag="OO369_003929"
     CDS             complement(1684..2904)
                     /locus_tag="OO369_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR5984006.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2908..3651)
                     /locus_tag="OO369_003930"
     CDS             complement(2908..3651)
                     /locus_tag="OO369_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR5984007.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3542..5008)
                     /locus_tag="OO369_003931"
     CDS             complement(3542..5008)
                     /locus_tag="OO369_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCR5984008.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5008..5241)
                     /locus_tag="OO369_003932"
     CDS             complement(5008..5241)
                     /locus_tag="OO369_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR5984009.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            complement(5229..5708)
                     /locus_tag="OO369_003933"
     CDS             complement(5229..5708)
                     /locus_tag="OO369_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984010.1"
                     /translation="MDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEIRLTPLTTENSILWMS"
BASE COUNT         1226 a         1618 c         1435 g         1459 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca actatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccggaacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat
     2161 ctctggtgct ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc
     2221 ctgcggcaaa tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt
     2281 catggtgtcg gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt
     2341 ttgatttgcc attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg
     2401 gacaagagcc acatggtttc cggtaatatc gacctgctcg gctttacctg gctcggtctg
     2461 ctcgtactcc gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat
     2521 ggcgctttcg tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc
     2581 gcgccggaca ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag
     2641 ccgcacattc ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga
     2701 ggcaagcgtg gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa
     2761 cttatcgggt ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc
     2821 tatcggcacg tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag
     2881 cctggtctca aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg
     2941 aacaaacacg atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat
     3001 gccgtccaaa gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga
     3061 atctcggaca tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat
     3121 cgctcgggcc atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc
     3181 ggccctagac ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg
     3241 accaccggtg acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc
     3301 ctcgagaggc agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg
     3361 gcccacagga gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg
     3421 ccgccaaact ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt
     3481 cgcccagggg gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact
     3541 gtcatttgaa ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg
     3601 taggctggtt cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc
     3661 cgatgccggg cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc
     3721 ctatgccggt tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca
     3781 gaccatcacc cagcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat
     3841 cgacatcaga catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct
     3901 tgccaagttc cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc
     3961 tctcctgcaa cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa
     4021 atcctttcgg ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct
     4081 gttctgcaaa ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa
     4141 acttatcccg cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt
     4201 cgatattctt cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct
     4261 tctccacgct gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag
     4321 cgctgtcgaa cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt
     4381 tctcggtcat cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt
     4441 gatgaatgcg ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt
     4501 tacccaggtc cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc
     4561 ggtcgagcgg gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg
     4621 tcattggtgc ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc
     4681 atttcagcac gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa
     4741 tgccacgatc ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat
     4801 ccgggatagc atcgactgct tcgccgatga tccaggatga acgataggac cattccacca
     4861 gcatgcggtt acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg
     4921 tctgcatccc tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc
     4981 gccgcagggc ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc
     5041 cagcgatgta tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg
     5101 tatctcgcgt tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa
     5161 atcacgatga ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc
     5221 tatgctgatc agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt
     5281 tctgaaacgg tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg
     5341 atgccgttgt actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa
     5401 ttcacacgtc gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta
     5461 gcaccggcag acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctccagc
     5521 gccgcctgat aagtacgctg gcagctcttc agtaattcat cttctttggc cttccattcg
     5581 tcggcccaga aaacaacgga tccgtgacgg taaacgttag cgcacttatc tgtaccactg
     5641 tcagccacgt caaagccaat acgctttctt ccactgggtt cgaaatttaa cgttttgtgc
     5701 gcatccactg cggcttctat ccaagacagt ttgatgat
//
LOCUS       DAJTSA010000173         5702 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000173 DAJTSA010000000
VERSION     DAJTSA010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.174"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..170)
                     /locus_tag="OO369_003934"
     CDS             complement(<1..170)
                     /locus_tag="OO369_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984011.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..2807
                     /gene="mutS"
                     /locus_tag="OO369_003935"
     CDS             246..2807
                     /gene="mutS"
                     /locus_tag="OO369_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR5984012.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2913..3565
                     /gene="pphB"
                     /locus_tag="OO369_003936"
                     /pseudo
     CDS             2913..3565
                     /gene="pphB"
                     /locus_tag="OO369_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3616..4383)
                     /locus_tag="OO369_003937"
     CDS             complement(3616..4383)
                     /locus_tag="OO369_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5984013.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4579..5487
                     /locus_tag="OO369_003938"
                     /pseudo
     CDS             4579..5487
                     /locus_tag="OO369_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            5484..>5702
                     /locus_tag="OO369_003939"
     CDS             5484..>5702
                     /locus_tag="OO369_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCR5984014.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
BASE COUNT         1410 a         1469 c         1481 g         1342 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1921 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1981 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     2041 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     2101 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     2161 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2221 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2281 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2341 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2401 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2461 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2521 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2581 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2641 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2701 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2761 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2821 tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta
     2881 taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca
     2941 atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat
     3001 tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg
     3061 gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct
     3121 ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg
     3181 atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca
     3241 agaggcaata gttctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
     3301 cgacataata aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg
     3361 caaagaaata gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa
     3421 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
     3481 taacattcag acgttcgcta accagattta tattgatacc ggatcgccga aaagtgggcg
     3541 gctgtcattt tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga
     3601 tgttatttca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
     3661 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
     3721 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
     3781 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
     3841 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca
     3901 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
     3961 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
     4021 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
     4081 tcccgcatcc agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat
     4141 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
     4201 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
     4261 ttgtaaatcc cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat
     4321 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
     4381 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
     4441 gcggcgcaac taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca
     4501 tctcaccata tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta
     4561 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
     4621 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc
     4681 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
     4741 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg
     4801 gcctaggtta aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt
     4861 acggcagtaa tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct
     4921 ctggctggat tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc
     4981 gctaacggtg aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggta
     5041 cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt
     5101 tcgaccgtaa aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa
     5161 gcgatggcac ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc
     5221 aatgccgccg gaaattcctg gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat
     5281 tacaccccgc attcagccgt cgatattttt gttaaggatc ttggtctggt tgccgataca
     5341 gccaaagccc tgcacttccc gctgccattg gcctcaacag cattgaatat gttcaccagc
     5401 gccagtaacg cgggttacgg gaaagaagac gatagcgcag ttatcaagat tttctctggc
     5461 atcactctac cgggagcgaa atcatgatca agattggcgt tatcgccgat gattttaccg
     5521 gcgcgacgga tatcgccagt tttctggtgg aaaacggtct accaacggta caaattaacg
     5581 gtgttccaac aggtaaaatg ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc
     5641 gctcctgtcc ggtggttgaa gccacacagc aatcgctggc ggctctgagc tggttgcaac
     5701 ag
//
LOCUS       DAJTSA010000174         5628 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000174 DAJTSA010000000
VERSION     DAJTSA010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.175"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            7..657
                     /locus_tag="OO369_003940"
     CDS             7..657
                     /locus_tag="OO369_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984015.1"
                     /translation="MHRIDTKTAKKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            629..1063
                     /locus_tag="OO369_003941"
     CDS             629..1063
                     /locus_tag="OO369_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5984016.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSVLIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1836..2885)
                     /locus_tag="OO369_003942"
     CDS             complement(1836..2885)
                     /locus_tag="OO369_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5984017.1"
                     /translation="MYNNKIDSNRKIRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSVFDYIKSKFYRLMIPYFTFAFSILIINTINSGETI
                     DYIYNHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFREIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
     gene            3336..4526
                     /locus_tag="OO369_003943"
     CDS             3336..4526
                     /locus_tag="OO369_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HCR5984018.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(4916..>5628)
                     /gene="gtrII"
                     /locus_tag="OO369_003944"
     CDS             complement(4916..>5628)
                     /gene="gtrII"
                     /locus_tag="OO369_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HCR5984019.1"
                     /translation="NFYNNPPTGLNLYKWLLIPLLILMFTLTYKLKTRSIYLISSIIF
                     IYILPVIFIVVVGSGAPPRLFVLMPIVAVILFSCLSNFRSIKYLNCMFFLFIIFNGVS
                     TSKNLFLNDTLARQKDISLAKEISYTSQTKGISLNGKYIYIYGSNDSGNMLSMSADTF
                     GKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQIEKIYPIVKSLPSWPNPDSIAEIN
                     GLVIIKLSEKKGWLPFNI"
BASE COUNT         1719 a         1049 c         1160 g         1700 t
ORIGIN      
        1 taatccatgc atcgtataga cacgaaaacc gcgaagaagg ataagttcgg cgcgggtaag
       61 aacggtttta cccgtggtaa cccccagact ggcacgcctg ccaccgatct ggatgatgac
      121 tactttgaca tgttgcagga ggagctttgc agcgtggtgg aggcatccgg tgccagcctg
      181 gagaaggggc ggcacgatca gttgcttacc gcacttcgcg cgctgctgtt aagccgcaag
      241 aatccgtttg gcgatatcaa atcggatggc acggtgaaaa cggctctcga aaaccttggt
      301 ttgggagaag cagctaaaag gaatgtaggt acaggggcga atcagatacc tgatatgagc
      361 ctgttcgcgt caattaatac cgtaacggct gctgcgcaaa aatttccgtc tggattaatt
      421 ttacagtgcg gtcagttgaa tggtgccccg aatgtatctt caacatacgg gatgaggttc
      481 ccgatgacgt tctcaagagt aattgctgtc gtagttacat tgaacgttac tggcgcggct
      541 gggcagccga ctgtatcggc gacaagtgtc cagaacactg gatttaatat tactgtgtcg
      601 cccggttcag gatacggttc atctgctgat gcgtattaca ttgcaatggg atattaacaa
      661 aatgtcatat ttttattctg catcgacaaa cggattttat tcgactgaat ttcacggcac
      721 caatattcct gatgatgcag tggaaatctc ggaatcagag tggaaaacac tgattaatgc
      781 acagagtgta acaaaaatga ttacctgtgg tgagaacggt catcctgtca ttgttgaccg
      841 tccttctcca acaccagaac aattagcctt aataaatgat gaaaagaaat ctgtactgat
      901 agcagaggca acgaatgtaa tagctccgct tcaggatgcg gttgatttag gtatggcaac
      961 agatgatgaa acgaaactgt tactggcatg gaaaaaatat cgggtgctgc ttatgcgtgt
     1021 taatgtagta aaacccgagt ggcctatgca tccaaataaa tagacttgtt aatctaggat
     1081 aattgttgga ataatactcg tttttcagta ggttcaggta gcctgggtat aatgctcact
     1141 tctacaatga tttcaactta ttttatttaa ttgatattac gaaacttaac tcatacataa
     1201 acaatatgac tcaatactgt cctaaatagc tgcgcagaaa agtgctccac tttcgctcag
     1261 cccttgctgg gcgaagcatc aggaaggcgt tctcagctag gttgtgttat atctggcttg
     1321 agttttgttg cccatacgca acacattacc catctgtaaa cgacaagtgt ggcagaatta
     1381 ggtgcagcta tttagactat atcgacgcag taacgataat agatatcgaa actgtactag
     1441 atattagcta cttatggtta ccggggggag tcttttgagc actggtgtta tcaattggct
     1501 tcaaataatt cgccaataaa ttatattgcg atagctcatc accttccagg ttgtttatag
     1561 ccagtttatc agcgtcacca cctactgttt atgcttttat ttctttgatt aataagtata
     1621 tgatatttta attcatctgg tgtatttctt tctcatgtgt tggtcgactg aatatctgtt
     1681 cagtgaaggg gtaattttat gtttgcactt cgaacattcc cttcagttgc taggaaagaa
     1741 aaccgtaaca tttgcgcatt aattttaaca gtataatctg agcataaata tgcgctatat
     1801 atctataaaa aggcgcaatt aattgcgcct ttttatcatt ttatcaaaaa tggcccaccg
     1861 actgtcctcg atagccaatg gccaatttta tctgatacat atacacaaat acaggtatat
     1921 atgattgcgc agataggatt tggaatagtc acgctgaagc ctataattgc taaagccata
     1981 gggataaata atttggtgat atactccatt ccacatatgt taagagtatt tcttccagct
     2041 cttacaataa agtcatttcc acaaattatt tttgctatga aaatattaaa gattatcaag
     2101 cctaccgtgg tgataatagt attaatagct tgaatataac tacttgatat tatgtctgca
     2161 ttgaatattg agataatctt aaaaagtaaa ggtttctggt taagtagttg atacgcactc
     2221 attatagcgg taattgagaa gacaatgaaa ccaattttag attttccaaa aaacctgtca
     2281 cgtgttaatt ctaaaaacat acatctaccg agaggcaata accaccagta tgccatagcg
     2341 gagtctatgt tccaaaacca ttgaggatct agtaatgggt tatgattgag cactgtttgt
     2401 gacaacatga acgataacag tgatatgatt aaaatcacta tattgttctt gactatttct
     2461 ctaaagattg catcaattgc tattataaca aaaaggcagt ttataaacca tattgttcct
     2521 acgaattggt tatttctaac accatatatt atatcataaa tgtggttgta tatataatca
     2581 atcgtctcac ctgagttaat tgtgtttatt attaatatag aaaatgcaaa agtgaaatat
     2641 ggaatcatta atctgtaaaa ctttgacttg atataatcga aaacagataa gtcatttttt
     2701 ttgatagtaa aaaaaccagc agcgaaaaag aatagtggga cgtgataact aaaaacaaaa
     2761 ggatacaatt tgccagcccc caacccaagg tgacctaaat atatagcaaa aataccgaga
     2821 aattttaaag catcaaccca atcatgtctt atttttctgt tgctgtcgat tttgttatta
     2881 tacatttaaa gtaggctctc cagttttgtg tgatttttaa acactcattg caaatatgaa
     2941 tgcctgaaag tttatgatta ttaatcaacg agttcaatat taatttatta tttttcaaag
     3001 tcgcactata taggcattta cttccgcagt accaccgata gtatcgatat caaatcagcc
     3061 tgcggccgcg ataactttcg taacattctg ccagcggctt tgttgaataa atcaggttca
     3121 agataatcgt ctctgcatca ggttagagtt tcaggcaata catcctcttt ctggcatacc
     3181 tgccactctc gcaccatggt catagccttt acgatctgca tactcaccag accagagccc
     3241 gctccctttt aggctgagga atccccagaa aagaagacag gcatagagtt ttgtgatcta
     3301 ctgattgcgc taacaaattc aatgagatca cctctatgcc tgcccgtaaa gtatgccaga
     3361 cttttttccg taatgcttta gcttccacgc atcagtatcg acaaaatgcc attattgatt
     3421 ctgctgctgc tttagtcggt ggcgcatctc tttctcttac cagcattggc cggcatttac
     3481 ccggtcctgc tcgcgtgaaa gataaaataa aacgcgttga ctgtctgttg ggtaataaac
     3541 gacttcataa tgatatccct ctgatattca aaaatattac atccatgatg acaaataaac
     3601 tgtcgtggtg tgttatcgct gttgactgga gcggttaccc ttttcaggaa tatcatgtcc
     3661 ttcgtgccag cctcctctgc gacggtcgct ctatccccct gatgagtcag gtcttcccgt
     3721 caaaaaaaaa taacgaggcg gtggaaatcg cttttctgga tgcgctttcc ggggccattt
     3781 ctccccggac tcgtgttgtt attgttactg atgctggttt tcaaagtgca tggttccgtc
     3841 atatcaaatc acagggatgg gattttattg gtcgtatcag gggggtagtg aagttcaggc
     3901 tggatagtga caaagataag tggctggaca taaaaatgtg cagggggagt tcagaggcaa
     3961 aatacctggg gacgggaacc ctggcccgga aaaaacgctc gcagtgtgaa ggacattttt
     4021 atctttataa acattcacct aaaggcagaa aaagcaggcg tgccagaggg aggccgggct
     4081 taccaacaac ggaaaaagaa caaaaagccg caggcagaga accatggtta atattcagca
     4141 atacagctga gtttaatgcc aaaaaaatca tgaaactgta cagtcgaaga atgcagatag
     4201 agcaaaactt tcgggatgaa aaaagtgaac gctttggttt tggccttcgg gcgagcagaa
     4261 gtcggagggg agagcgtttt ctggtattaa gtctactggt tacactggcc agtatcgtac
     4321 tctggctact tggttatcat tttgaaaata aaggatttca tttaaagtat caggctaata
     4381 gtctcaaaaa acgaagagta ttgtcttttc tgacgctggc tgaaaacgtc ctgagattta
     4441 accctgaact tctacgacga gcacaacctg aaaagatatt gtgccggttg gccagcactt
     4501 accgaagtat ggtgctggct tactgatgaa aattttctgg ggatccctca gcctttcggg
     4561 gaggaatgag ggctctgata ttcttttgcc gtagttcatc gtgaaaccgt cggatgtcat
     4621 ttggcctgag tgacccatgc ctcccaaaag cagagtcgct agtgttatgt aattaggcgg
     4681 ttaaacttca attctcagtg ttaagtttgg gtaaacgctt gtaaatggta ttgctaattt
     4741 tttaattgac taatattaga aacggttagt gtgattgtat atagatattt gagtgtaagg
     4801 tatttaaagg ggcattcagc ccctccgtct atttgtgatt gcatccattg atgtgtgtat
     4861 tattttgagc ttgaaaaagg gaggcgctct ttaataataa ataaatctat caaagctaaa
     4921 tattaaatgg aagccaccct tttttctctg atagttttat tatcaccaaa ccatttattt
     4981 cagctatcga atctggattc ggccaggaag gtaaactctt cacaattgga taaatcttct
     5041 ctatttgttc tttatttgct ggtttgcaat tacagattcc atagtaattc ataaatgcaa
     5101 ccatcctaaa atagttgcca ccatcccacc aaaaaaaaga ttttccaaaa gtgtctgcgc
     5161 tcatggagag catatttcct gagtcgtttg aaccatatat atatatatat tttccgttaa
     5221 gggaaatgcc ttttgtttga gatgtgtatg atatttcttt agctaaagag atatctttct
     5281 gtcttgcgag agtatcattc aaaaatagat ttttggatgt tgaaacgcca ttaaatataa
     5341 taaataaaaa aaacatgcag tttaggtatt ttatagagcg aaaattgctt aagcaagaaa
     5401 acaaaattac tgctactata ggcattaaaa caaacaggcg aggtggcgcc cctgagccaa
     5461 caacaacgat aaatataacc ggaagtatat aaatgaaaat aattgatgaa atcaaataaa
     5521 ttgatcttgt ttttaattta tatgttaggg taaacatcag aattaataaa ggaatcagta
     5581 accacttgta taggtttaaa ccagtgggag gattgttata gaagttat
//
LOCUS       DAJTSA010000175         5601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000175 DAJTSA010000000
VERSION     DAJTSA010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.176"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..411
                     /locus_tag="OO369_003945"
     CDS             <1..411
                     /locus_tag="OO369_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5984020.1"
                     /translation="AVRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDA
                     LCYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGI
                     DPEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            443..1057
                     /gene="istB"
                     /locus_tag="OO369_003946"
                     /pseudo
     CDS             443..1057
                     /gene="istB"
                     /locus_tag="OO369_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="OO369_003947"
     CDS             complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="OO369_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HCR5984021.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1378 a         1402 c         1332 g         1489 t
ORIGIN      
        1 gccgtcaggc agcagcgaag caaagcgtta ctgacgtcgc tgaatgaatg gatggtggag
       61 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
      121 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca
      181 gcagaaagag cgcttcgtac cgtctgtctc gggaaaaaga attacatgtt cttcggcagc
      241 gatcatggag gcgatcgcgg agctctgctg tacggactaa tcgggagctg tcggttgaac
      301 ggtatcgatc cagaagcgta tctgcgccat atcctgagcg tactgccgga atggccttcc
      361 aaccgagttg acgaactgct gccatggaac gtagttctca ccgataaata agcgtcaata
      421 cggccttccg ttgacgctta cagaactcct gtggcgcgag agtgaaaaac tgcgtcgtta
      481 taaaaaggag gcccggttgc cagttgccaa aacgctaagc gaatacgact tcattcagtt
      541 gccggaactg aatggagctc agttccagca actctgtgaa acgacagact gggttgatgc
      601 tggagaaaac gttctgctgt tcggggccag cgggttgggg aaaagccatc tggcggcagc
      661 gatcgtggat ggcgtagtag gccagggcta ccgggcccgg ttctacagcg caggagagct
      721 gttgcaggaa ctacgtaaag ccagagctca gttgaaactg aatgagctgc tactgaaact
      781 ggatcgctac cgggtgatag tggtggatga tcttggctat gtcaaacgcg acagcgccga
      841 aacgggtgtg ctgttcgagt taatagcgca tcgctatgaa cgtgggagcc tggtgataac
      901 cagtaaccat ccgttcagca tgtggggcag catcttcgtg gatgagacta tggcggtggc
      961 ggcagcagac cgactgatcc atcacggata tatgttcgaa ctgaaagggg aaagctacag
     1021 gaaaaagaca gcgaaggcag taacaagcgt gacttgatgt cgcactgaag gggcggccag
     1081 tatagttggc gccagtcggc aaaactagtt gacgtctaat acctggacgg tcacttctga
     1141 gaaaaggcat ttacacagaa tcttaaacag gctcacggag ttctttcaga aaaaggcctg
     1201 ccgaatggca gacctatccc attcgtcaga aaacataacg gaagttggcg tttatcgcat
     1261 tatccacatt atatttcccg aacgctgatt tctccagctc cagtcccaaa cgcatattat
     1321 ccttaatttc cgcattcatc cctaccgtca tcagcatcct gctatctttt tcaccttcga
     1381 aacgtttctc tccggaagca tcctgcagaa ccgtttctcc gttcgccagc aggtcgaact
     1441 gataacccag cccggctcga gctgtgattt tccagtcgtc tccggagaag gctcttccca
     1501 cgtcaacacc agtacggcca atcagtgggt tataatcctt gtctttcatg ctcagagcca
     1561 ttccccggtc ttcccagcta aaagcttttc ctgatacaga accgtaaacc agctctatct
     1621 gtggctccac ccaggactct ttcgtcaggt ggtaacgata accaacttca gcaccggcat
     1681 accaggaatg agagctgtag tcttttgttc ccagtgaggc aaaattcgcc gtgtgctgat
     1741 tatcatgatg gagatattta ccaatcaggt caaaataagc tccggaatta aagagtgcag
     1801 aggcatacag accgccaccc acggatttgt tttttccact gaatgcgtgg ctgcttgcat
     1861 tgctgtccgt ataggtcagc aatgcccccg taaataaatc cacaccgtcc agctcatgct
     1921 ttctgtcgac accaatctga acgtgagtgt agttatcgct gtagtcacca tctgccgaac
     1981 cggtaccatt cattatgcgt gcccagacac cggcatcccc ctgagtatcc cgcaggtcac
     2041 ccatacgttt gttcaggtta ttgacctctg taaggaagga cttataacct gatgccataa
     2101 agtcttttgc ggctttcgag gctccggcat cagcggtagt ctgatatcct gtcagcaccc
     2161 attttgtggc atcatccgtt ttttccgtgc tgattactgg cgtaacgtta ctgaaaccaa
     2221 tctgctgcgt accggcagtg aacgttttct catcactccc tttcggtgcg gtaattaacg
     2281 tcacgctcat ttcactggct ggtttgttta aaaagtcaac caggacgcta ttattactgc
     2341 ctgacagttt gtctgtgaca ttcaactggt cagcccggct gttgttggta tgcatcagga
     2401 acgtggtatt atctgcagaa agctctttta cttgtaaagt ggcaaagtgt ttcccgtccc
     2461 ccagttcaat tcgcgagccc ttagccttca gtgcaccggc ctctgatttt ccgtcagagt
     2521 accacagagc attgatcata tttaccgtac tctgtgcccc cgtaatactg cctctcagaa
     2581 cagcgttata tcccttcatt aaggagctga ataacggaga gtccgtcttc cctgcatcag
     2641 catcactgtc tcccagatta atggttgccg cagtggttcc tgcatgaata tccgaactca
     2701 gatagcccat atttttaaca gaaaagctgg cgttatcttc gaggttaatc ccttctgttg
     2761 tagaaattac gggtgagtaa tacccctgtt tctgcgcaga aggcatcccc gtcagggtca
     2821 gagaagcatt ttcttttaca tccacagccc cggtgctgaa aataccgctc tgagccgtaa
     2881 gttttgctcc ttttccgagg gtcaggctgc tgttgacaaa gctgccaaca cggtcaaaaa
     2941 cagcattctg agaggtcaca ctcacggtgc tatcataagc ctcaatacca ccggtgaatc
     3001 tctccatgat ttgcagggat gatttatttt ccagcgtaac atttccgctg taatcactct
     3061 ggtcaccaac tgtggttgca gtggcactcc cttcaacaga ggagaccgca ttaccggttc
     3121 cgtcactcct gtcagtaaag acacggctac ttcccagaat gactgtactt ccattggtgg
     3181 cgttaatatc tccctgcatc ttgccgtttc tggacagatg gaatgttgcc gcatcgagtt
     3241 tcagtttttc cagcgtaaac tgacgggttt cccagtcgct ctggctggaa gaagtcgtcg
     3301 ttccagcatg aataactgga tgaccctgga atatcagcgt gccggacttc ttggttatcg
     3361 aaccttctgg caggttaact gaaccatcaa gagccagtac atccttacct gagagctgcg
     3421 gaatattcac atccatattg ccgtttagtt ggccgtgata catataaggc tggctgctgg
     3481 cctcaatttt ttgttgcttc acagtatcaa tggctttatt acgatctttt ccgacattct
     3541 gccagacact gctgttgttc agatcggaga agaacgggga atatgaactc gctttcagga
     3601 tgaaatattg cttggtagaa ctgtcataat ataggtctcc cggcgctcct ctccccccaa
     3661 aaatactgac agtattaacc ggtacattga tgtcactggc ttttaatgtc tgaaggtcga
     3721 gtgttaccgt tgattttttc tcactgttat tggtaatgat ggccccgtag tcagccgcct
     3781 gaagacgggt aaaggtcaga ttattaccat tcagttccag acgtcctcca cggtatcccc
     3841 acgaaatatt atccggatta acttgctgtg agtcagaaag cacaactgtc ggacgaccgc
     3901 tggcaatacc aacagagctg aaagcctgta cctttccgtc agcatccgcc tgctggttga
     3961 gaataacggt accgtccccc accttgagtc cgcctgcatt cacaccggtc ccgtttactg
     4021 tcagggtacc ttcgccagtt ttatgcaggt tatcaccggc aactccgttc acctgccaca
     4081 gaacgtgggt tcctttatcg gtgataatcc cgccccccat ccatgtctgg ccgttcaggg
     4141 cggacaccct gtaatcgccg gcaaactgca gatacccagc tccctgatta acggaattct
     4201 gcagaacgac ctcgccatta ttgccggtga acaggaggtt ttttcctgcg ttcagatcat
     4261 ttcctttctt tccatgcata tcccaagtcg tgtttccctg agtcagtgtg ccggtgccat
     4321 tagccgcatc atatttccac tgcagtactc cttcaccaga ggtgtaggct atagttttgt
     4381 caaaatcatt ttgcggttgc tgaccaagga aatcctgagt ggtgacgacc cagttatttc
     4441 catagttagt tcctgaggaa agcacgccaa tcagtaccca ttttttttgc agggaatcat
     4501 aagcaaacag gggggagccg ctgtccccca tattcagata attagccaga ggtccattcg
     4561 cgggattaaa aatgtcaccg gtctgagctg taatcatctg tccgttgtta taggagttca
     4621 gcgcgctgac ggttccccca acaagaaatc cgccattggt atacacttgg gtgcgattac
     4681 catttttgtc tttgacgtac tgcatccccc ccccaaggcg atagaattca gtaaagcgtc
     4741 cgccggcctg ataggcaccg ctcacagccc cgatgtcaga cacttctgcc ggcgccacct
     4801 ctgtcaccag tttactcagg cggcgggttt taatatcaag accgctatta ttatttgtac
     4861 caaccgcagt ataggtgttt ttggcgtatc cgaagctcat cgtgtcagaa ccgctgacat
     4921 gtttggcggt aaccacatac tgaggggagt aaagtgtatg gtctcccgga ggcaggctac
     4981 cggtagtaat agtggcacta ctgaaatcag ccatcggggc tttatcaagt ttaccgacca
     5041 gattgccctg cttgtcgtaa atggaaatat tcgtggtccc cggcgtaaac tgacctttat
     5101 tctcggcaaa gtcgcggaat atctgataag ggatctctgc actgaccgtt gctgacagag
     5161 ctggccatgc cgaggaaagt gataaagctg caacagacgt aagaataacc cgacgtgaaa
     5221 gtctgcgatg gcttttgcag gtaaccctgc gggccagttc ggagacagca atcaggcttt
     5281 tggttatatg gcaatactta agataatata ttttgttcat atagaaacat caacctaatt
     5341 ttgacaaaga ttaattacat aggatgtaga tggagcagtc cgagattcta ctaatatcaa
     5401 ttattaagca tctgtttttt agttggtgta atataatttt tattaaggta agcggaaaaa
     5461 caaccaaaaa aaacgcaaga tgttattttt tttatcattt tgagccagtt tggctggggg
     5521 gatactcttc tcttaagctt cttgctcaac tttaaaaata gttatgccag ttcggtatcg
     5581 ataaggggca gtttggatat c
//
LOCUS       DAJTSA010000176         5557 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000176 DAJTSA010000000
VERSION     DAJTSA010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.177"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..641
                     /locus_tag="OO369_003948"
     CDS             <1..641
                     /locus_tag="OO369_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCR5984022.1"
                     /translation="VGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAITT
                     GKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNA
                     VLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCAL
                     GLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(695..1300)
                     /gene="azoR"
                     /locus_tag="OO369_003949"
     CDS             complement(695..1300)
                     /gene="azoR"
                     /locus_tag="OO369_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR5984023.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1501..5403
                     /gene="hrpA"
                     /locus_tag="OO369_003950"
     CDS             1501..5403
                     /gene="hrpA"
                     /locus_tag="OO369_003950"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR5984024.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1376 a         1361 c         1539 g         1281 t
ORIGIN      
        1 tcgtaggcgc gtgcattgtg ttgatggagc taatgatgat ggttcagtta tggtggtcag
       61 tctggttatg ttggccagta ttatcgctac tcattattgg ccgtgggtgc ggtgggcttg
      121 gcgcgataac aacagggaaa gatagtcagg ggaaactccc gcccgccgtt tactggctga
      181 cattgccctg gcgcatcggg atgtggctat ctatgcgttg gttttgtcgt cgcctggagc
      241 cggtgagcaa aatgactgct ggtgtttatt taagggcgtt tccacgacat attccggcac
      301 agaatgcggt tctggacgtc acctttgaat tccctcgtgg acgagcgaca aaagatcgac
      361 tctatttctg tgtaccgatg ttggatctgg tggttccgga agagggggag ctccgacagg
      421 ccgtggcgat gctggaaaca ttacgcgaag agcaaggcag cgttctggtc cattgcgcat
      481 tgggattatc gcgcagtgcg ctggtagtgg cggcatggct gttatgttac ggacactgta
      541 aaacagttga tgaagcgatt atctttattc gagccagacg ctcgcatatt gtgcttaagg
      601 aagagcacaa agcgatgttg aaattatggg aaaacaggta agtggattga gatgtggact
      661 gaatatctac agtccacatc aagaccgtgt ccggttatgc agcaacaatg ctgtcgatgg
      721 ctgcttttgc gtcagactgt gctttcgctg ccatttccgg accgtatgcg atcccttcgg
      781 cgaagacaaa tttcacatcg gtaatgccga taaagccgag gaacgtggac agatacggcg
      841 tcaccaggtc cgttggtcca tctttgtgga tcccaccgcg gctggtaata acgatggctt
      901 ttttacccgt taccagacct tccggaccgt tctcggtata gcggaaagta acgcctgcgc
      961 gtgcaaccag gtcaaaatag tttttcaact gagttgagat gttgaagtta tacatcggtg
     1021 ccgcaataac gataacgtcg tgggctttca gctcggcaat cagctcatcg gaaagcgcca
     1081 gagcttcctg ctgacgcgga gtcagcggcg catcgctcgg acgcagagcg ccaaccagtt
     1141 cgccatccag taccggaatc ggatttgcag ctaggtcgcg aacggtgatt tcatcagcgg
     1201 agtgcttttc gctccattgt tcaacaaaat aatcggacaa ctgattagac tgagagtacc
     1261 ctgccaggat gctggattta agaactaata ccttgctcat ggtgtttcct tatagatgtt
     1321 tgaatggacg atgccccgtt gcttgctgac actttattca caatcctgcc acagagatcg
     1381 cgcaataaat cgaagtctat gttcgaattt attgaacaac gcatagaaag ccgcgatgtg
     1441 gtactctata tctatcattt aaaagaaaat taatcaggca gactactgcc cactaacgtt
     1501 atgacagaac aacaaaaatt gacctttact gccttgcaac agcggctgga ttcgctgatg
     1561 ctgcgcgaca gactgcgttt ttctcgccgt ctgcattgtg ttaagaaggt taaaaatcct
     1621 gatgcacaac aggccatttt ccaggagatg gcgaaagaga ttgaccaggc ggcagggaaa
     1681 gtcctgctgc gtgaagcggc acgaccggaa atcacttatc ctgacaattt accggttagt
     1741 cagaaaaaac aggacatact cgaagcgatt cgtgatcacc aggtggtgat cgtcgccggg
     1801 gaaaccggct ccggtaaaac gactcagcta ccgaaaatct gtatggagct ggggcgcggt
     1861 attaaagggc tgatcggtca tacccagccg cgtcgcctgg cggcacgaac ggtggccaac
     1921 cgtattgcgg aagagctgaa aacggagccg ggcggttgca tcggttacaa agtgcgtttc
     1981 agcgatcacg taagtgataa cacgatggtc aagctgatga ccgacgggat cctgctggcg
     2041 gagatccagc aagaccgcct gctgatgcag tacgacacca tcattattga cgaagcgcac
     2101 gaacgcagcc tgaatatcga ttttctgctc ggctatttga aagagttgct gccgcggcgt
     2161 cctgacctaa aaatcattat cacttccgcg actatcgacc cggaacgctt ttcgcgccac
     2221 tttaataatg cgccgattat tgaagtctcc ggtcggacct atccggtgga agtgcgctat
     2281 cgcccgattg ttgaagaagc cgatgacacc gagcgcgatc agttgcaggc gatttttgac
     2341 gccgtagacg aactaagcca ggaaagcccc ggcgacattc tgatctttat gagcggcgag
     2401 cgggaaattc gcgataccgc cgatgcgctg aacaagctga acttacgcca taccgaaatc
     2461 ttgccgcttt atgcgcggct ttcgaacagc gaacagaacc gcgtgttcca gtcgcacagc
     2521 ggacggcgca ttgtgctggc gaccaacgtc gcggaaacct cgctgaccgt accggggatt
     2581 aaatacgtca tcgaccccgg tacagcgcgt atcagccgct acagctatcg caccaaagtg
     2641 cagcgtttgc cgattgaacc catttcgcag gcgtctgcca atcagcgtaa aggccgctgt
     2701 ggtcgtgtgt ccgaagggat ctgtattcgt ctctattccg aagacgattt cctctcgcgc
     2761 ccggagttta ccgatccgga gattctgcgt accaacctgg cctcggttat tttgcagatg
     2821 accgcgctgg ggctgggcga tatcgctgcg ttcccgtttg tcgaagcacc ggataaacgc
     2881 aatatccagg atggcgtgcg tctgctcgaa gaactgggcg cgatcaccac ggatgaacag
     2941 gccagcgcct ataaactgac gccgctcggt cgccagctct cgcagttgcc tgtcgaccca
     3001 cgtctggcgc gtatggtgct ggaagcgcaa aaacatggct gcgtgcgtga ggcgatgatt
     3061 atcacgtccg cgctctccat ccaggatccg cgcgaacgtc cgatggacaa acagcaggca
     3121 tcggacgaaa aacatcgtcg cttccacgac aaagaatccg acttcctcgc gtttgtaaat
     3181 ctgtggaatt atcttggcga gcagcaaaag gcgctttctt ccaacgcctt ccgtcgcctg
     3241 tgtcgtaccg attacctcaa ctatctgcgc gtgcgcgaat ggcaggatat ctacacccag
     3301 ttgcgccagg tggtgaaaga acttggcatt ccggtcaaca gcgaaccggc ggagtatcgt
     3361 gaaattcaca tcgccttact gaccggtttg ctttcccata tcggcatgaa agatgccgat
     3421 aaacaggaat ataccggtgc acgtaacgcg cgtttctcca tcttccccgg ttctggttta
     3481 ttcaaaaagc cgccgaaatg ggtaatggtg gcggaactgg tagaaaccag ccgcctgtgg
     3541 gggcgcattg ctgcgcgtat cgacccggaa tgggtagaac ccgttgctca gcatttgatt
     3601 aaacgcacct acagcgaacc gcactgggaa cgggcgcagg gcgcggtgat ggcaacggaa
     3661 aaagtcactg tttatggttt gccgattgtt gctgcgcgca aggtcaacta cagccagatc
     3721 gattcggcgt tatgtcgtga actctttatt cgccacgctc tggtggaagg tgactggcag
     3781 acgcgtcacg cattcttccg tgaaaacctg aaactgcggg ccgaagtgga agagctggaa
     3841 cacaaatcac gtcgccgcga tattctggtt gatgacgaaa cgctgtttga gttctacgac
     3901 cagcgcatca gccacgatgt aatctccgct cgtcacttcg atagctggtg gaaaaaagtc
     3961 agccgcgaaa cgcctgattt gctcaacttt gaaaagagca tgttgatcaa agaaggggcg
     4021 gaaaaaatca gcaagctgga ttacccgaac ttctggcatc agggcaatct caagctgcgt
     4081 ttgagctatc agtttgagcc aggcgcggac gcggacggtg tgaccgtaca tattccgctg
     4141 ccgttactta accaggttga ggaaagcggg tttgaatggc agatccccgg tctgcgccgc
     4201 gaattggtga ttgctctgat taaatcgttg ccgaaaccgg tacgccgtaa ttttgtgcct
     4261 gcaccaaact acgccgaagc gtttttaggc cgcgtcacac cgctggagtt accgttgctc
     4321 gacagccttg agcgcgagtt acggcggatg accggcgtta ccgttgaccg cgaagactgg
     4381 cactgggatc aggtgcccga tcacctgaaa atcaccttcc gcgtggtgga tgacaaaaac
     4441 aagaagctaa aagaagggcg ctcgctacaa gatctgaaag atgcgctgaa aggcaaagtg
     4501 caggaaacgc tgtccgcggt ggcggatgac ggtatcgagc agagcggctt acatatctgg
     4561 agttttggtc agctgccgga aagttacgaa cagaagcgtg gcaactataa agtgaaggcg
     4621 tggccggcgc tggtggatga acgcgacagc gtgacgatca aactgtttga taacccgctg
     4681 gagcaaaaac aggcaatgtg gaatggtctt cgccgtctac tgctgctaaa tatttcctcg
     4741 ccgatcaagt atttacatga gaagttacca aacaaagcca agctggggct gtactttaac
     4801 ccgtatggca aagtgctgga gctgatcgac gactgtatct cctgcggtgt ggatcaactg
     4861 atcgacgcca atggtggccc ggtctggacg gaagaaggct ttgctgcgct gcatgaaaaa
     4921 gtgcgcgccg aactgaatga cacggtggtg gatattgcga agcaggtcga gcaaatcctc
     4981 actgcggtgt tcaatatcaa caaacgtctg aaagggcggg tggatatgac catggcactg
     5041 gggctttctg atattaaagc gcagatgggc gggttggtat atcgcggttt tgtcactggt
     5101 aacggcttca aacggctggg cgacacgctg cgatatttgc aggcgattga aaaacgactg
     5161 gaaaaactgg cggttgaccc acatcgcgat cgtgcacaga tgctgaaagt cgaaaacgtc
     5221 cagcaggcgt ggcagcaatg gatcaacaaa ctgccgcctg cacgtcgtga ggatgaagac
     5281 gtgaaagaga tccgttggat gatagaagag ctgcgcgtta gttacttcgc tcaacaactt
     5341 ggtacgcctt atccgatttc agataagcgt attttgcagg cgatggagca gattagcggt
     5401 taaccctgct atttgcctaa taaatctaaa acccggtaag catttagcgc cgggtttttt
     5461 attaattcta aaacggtaag gacaaaaatt caggaattca gaaaaataca attctcagct
     5521 gcaagatgaa taatgtttat ctacagcatt tccttaa
//
LOCUS       DAJTSA010000177         5485 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000177 DAJTSA010000000
VERSION     DAJTSA010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.178"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1630)
                     /locus_tag="OO369_003951"
     CDS             complement(<1..1630)
                     /locus_tag="OO369_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCR5984025.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPW"
     gene            complement(1646..2530)
                     /locus_tag="OO369_003952"
     CDS             complement(1646..2530)
                     /locus_tag="OO369_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5984026.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(2541..3146)
                     /locus_tag="OO369_003953"
     CDS             complement(2541..3146)
                     /locus_tag="OO369_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5984027.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3317..>5485)
                     /locus_tag="OO369_003954"
     CDS             complement(3317..>5485)
                     /locus_tag="OO369_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HCR5984028.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSHYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1580 a         1364 c         1142 g         1399 t
ORIGIN      
        1 cccacggttt accatcttta tgactggtta acaccccggc aatcagctcc agttgtgggt
       61 gccacggctg cccggtgtag atgagaatgc cacccggttc gatggcattg gctagacctg
      121 cgagtgagtt ttttacctgc tcattttcgg gaaaaagctc atataggcca gagacaatcg
      181 ccagcgtagg ccgcggagtt aacgcgctga gttcctccgg gttaaacgca tcgccctgtt
      241 caaaacgcac ccgcccagac attccccgtt gggcaatcat ctcttgcccc tgtgcaacat
      301 ttaactcact gtaatcacgt aacaaaatat cgcttacggc aggctcgttt gccagcgcat
      361 ccagtacata gcgtccatgc cctgcggcaa tgtcaaccac gcggacggct aaacctttgg
      421 cgtggagatc ggcaacggct tgtttaatca atatttgcaa atgggttttg cgctggcgaa
      481 taccgcgcca tccaacactg ttcaggtaga ttttgtcgat taaacgcccg aatgcgttac
      541 tgccttgcgg ttgattttga tagacataat ccagggaact gcctgaatca aagccggtac
      601 tcattccgag atgcaggccc gcagagtgcg caccgaacaa tttcatcgcc tttcgcataa
      661 agcgatacgc taaatcaacc ggcgataatg gcacgggtcc accagaaagg agccgccatc
      721 gatccgctga tggtcctgtg cggtcttcat gctgataatc aaatttttgc gatttgttag
      781 catataagcg gcagataaag ctttgcattt tttcaaatgc ctgcgccctg ttctcttcac
      841 ccaatgtgtc gtgataaaag cctggcagca gatgcagctc tttcagaggg ctacgtaatc
      901 tctgataaaa atcaatctgc ggttggcgat gcaccacata gtcatcacct gatatcagaa
      961 gttgcgtggg gagcgtaatc gccgccgcat cgctaacaat acgttcagac gttttataga
     1021 gatcgagcaa gatattgacg gcaatcgccc gtgtgatcag cggatcatta ttgaaactcg
     1081 ccacccgttg ccgatcgtgg gtcaaatagc gccctttcac ataggaatta ataaaaaata
     1141 gaccacgcag acgatgccat aacgccagcg caggacgtgc cagcggcaca tacaatttaa
     1201 ccttaaaggc cggagaagcc agcaccagcc cacgaattgc aggtgcataa tcatgaaccc
     1261 atgtggcaac aagcactgcg ccgacgcttt gcgcgatcac taccacctct tccagtccga
     1321 cctggctgtc gctggcagca aaacggacaa attcatcgac atcctgcact gagcgcataa
     1381 gagacgggct ataaccacgc ggccccgaat tttttccatg cccccgggca tcccaggcat
     1441 aaaaagcagt atcaggcatc gccagttcat caacgagatg ttgtagacga ccagaatgtt
     1501 catgcccgcg atgaaataag acgatgacct ttttcgcccc tggctgtaaa gtgggccagt
     1561 gccgataata caatgctgta ttatcactgg tggtaaaaaa atgttcccca gggatgcgtg
     1621 aattttccat tttctctatt ttccttcagt aacagcagta gcgaataaaa taaaaaaata
     1681 ccggagcggt gaaaattaat gagtcaatcc tgtcaaggag tccgccatgt cctagtaata
     1741 gttttccact atctttaaca ccaatatctc gtttgatggc tgacatcact acatcgccgc
     1801 aaaaaccact aataccaatt aacaaacccg ctaataatgc ctgtaatgta tttagcggtg
     1861 tcagcaacgg tccaataatc agtgacgcaa tcatggtggt gattacgccc cccagcagac
     1921 cttccagcgt ttttcccggg ctgactttgg ggaccacttt tcttctgccg caggatttcc
     1981 cccataaata ctgtgcaata tcgtttgact cggttaacgc caaaagaaat aacaccagta
     2041 acgccccgcc ctggatattt atggttggca acattaatag ccaggcggca tgactcaagg
     2101 caaaaacggt cattatccat ccccaaaaaa tggcgctcac ggtatgctga aaaccggagg
     2161 ggtcacccac aaaaactcgc caggtggcta atatcagaaa cccagtcaaa ggtataaata
     2221 acagaaacag ctcaaagcaa ttaaaaccga taagcaaata gttaagagga ataacccaat
     2281 ataaccaacg cgaaaaatgt acggatataa gcgtacaata ttctttcaat gccagaaaac
     2341 ttatcagcgt aaaaaacgtc aacgtcatcc agcgtgggcc tgaaatcacc agtgaaaaac
     2401 aaatgacgat gccccaccag gtacgaatac gtaatgtaac ttcatcccct tttctctcag
     2461 gtaaccgcca tagcagaaaa cggtttatca gcttgctaaa attaacaacg cgaaaagtgt
     2521 tgccagagat ttttccagca ttatctttca gcgctcataa gaagcacact ccgacaacga
     2581 ttaatcgcag tacagataag cagaattgaa gcaatgctcc ataaaagatt attccattgc
     2641 atccactgtg gataaatggc aacggccaga ccccacagac caaatattaa cgcgcgatca
     2701 cttttgccaa atggaccggc ataactgcga acaccgttaa tcgtctgcgc gagtaaaccg
     2761 caaaactcgg tcaatatggt gcaaaataac atgagtatca cgagtgatgc gttactttcc
     2821 ggtaaaaata aaaacggtaa atagagcgca atatcggaaa taacatcgcc agtctcattc
     2881 aaaatcgccc ccagacgtgt ttgctgattg cactcacgcg ccaacatgcc atccagcgca
     2941 ttgagcgcca tacggataaa aagcacgatg ggcaatagca aaaagaggat gggttgtgcc
     3001 accaacacca acagcaatcc ggtaaaaaga gaaagcgcca gtgccgtaag agtgatgtga
     3061 ttcgctgtaa cgtggtgctt ataaagccaa aacatcgtcg gccttaacag cgactgaaag
     3121 agcggtttta tctggtagag cgtcattatc aatccttgat aattcggttt gcgctatggt
     3181 gcatcaattc ccctgacgga gatatcgcaa cacgccgctt cattaaaaat tcagaatata
     3241 tcaaggtatt gggggctcac tgtcaggaat gattatcccc aataaatcaa gtagttaaaa
     3301 attattacgc tgttattcaa tacaactcat caagtttcct catcgcttgt tcccgtattg
     3361 cgcgcgtctt accgttaagc gccagctggc ggactaaatc cagttcaggg ttaacaagct
     3421 ctaacgccag caaaggatca cgtttcagcc tcgccttcaa tacatcaact ggaaagcggg
     3481 gattattcat cgccacaatc caccactcgg gatcaatttc tgccgccagt gccgcagagg
     3541 gggtgtggcg gttgctaagt gcgccgaact tgaaaaatgt gagattgctt ttcgccagtg
     3601 tactccacca tatacgatcc ggttgttttg ccagtacatc cagttgtgct aacgcatcgg
     3661 ggtcgagtgc ataataaaca cgtaatgctt catcaacctg cgcacgggag ctattttcct
     3721 gcctcagaaa aaacttcgcc caaatatcat tgcgtggcgt gttttgctgt aattccttga
     3781 gaacaacagc gggtaaatta tcccaactaa ctaatgcact ctctaaaaag tcccagtctt
     3841 ccaaacgtat cagggcggcg gcatttaata gcatctggtc atcaacttcg ccgtggctaa
     3901 tcagctcaga caaaatcata tcagcgctgg tttcatatgc atctgaatct gctttcggat
     3961 tatttttccg atgcgataaa gcacgttgcc aaattttttg tttcatcgac gtgctcagat
     4021 actgattttt cgccagatta ctccacccct caggatcgtt gaaacgtgtc aataaccgtt
     4081 gtaaagcagc gtcagagtga attacctgca tatcccgaca gcaaaagtag gcaggggatt
     4141 ttttctccat ccttttaaca aaattaaact gtaagttatc tggcaatgct aaaaataaca
     4201 cacgtattag atcatcgtca ttctcgaatt tttggtataa ataggctgca actttgtcac
     4261 tctcttgtgg cgtcatccgc aataattgac catggtgttt catctctgcc agcttgaacg
     4321 ctaagcttgt gacaacattg tttgatgaat cgttcgccaa ctgcattgcc tgattgtaag
     4381 tgagttgtcc tccagcaccg acctgacgaa tagaggaatc atcactgcgg atatagccat
     4441 caataatgga ttcgggcaat aaccacgcat ctttataaag ctcctgaatg atcatatccc
     4501 aatctctttt cgccagagtc atgacgatat ccagagggag attttcacgt agcgcaagat
     4561 tcaacggacc gacaccatca ggtagacgac tcattcgcgt cattatttcg acagagagcg
     4621 gttcacggtc gagcaacccc agaacaacag gctggtaacc caattgctga tcggtcaact
     4681 ccagaatctc attgtcaggc atatatctcg ccatactttc aatcgtttgt tcatcattgc
     4741 tattaataag ttgtttgcgt aggttagctg aaattccttt aagccttggg agagttcgca
     4801 ttatggcaag atcaaatgtc gcaatatctc gtccttcaaa tgcagcaacg ttgccttcat
     4861 ccagcatttg caattcctgc actaactcag gagaaggctt agggcgaaac agcgcttctt
     4921 ttttcatctg attacagaca ttccctgcca tgcgaatatt gcttcgctta aaaatttctt
     4981 tcagagcatc ttcatcattt attagacccc aataatatgc atcaggattt attatcatca
     5041 gcgctttttt gacattttca gtgtcatttt tgtcactcgc acggaccatg aattcactat
     5101 ttgtcgaaga cgcaacaaca ggtgcaatta tgtcattgat aaatacccca cgcgcattaa
     5161 tactcccctt aatcgtagac ggattatcgt aatgtgagtc gataaatacc agtccacttg
     5221 catttatcga accggtaatt aacagtggtg aatccgtaac tatattcgtt gccgtcacgt
     5281 taccgagaat aacccatgga ttagaaagag agaaagtgga aatattacct ttgactgtca
     5341 aattgccaag aacaatcaat ttttgattac tctctatatt accttctacc actaaatcgc
     5401 cacgatgcac tttccagtcc gatgcagata aaccgaaggg gacaagattc acgctactgt
     5461 tgatagcttg ttcaacttgt tgttt
//
LOCUS       DAJTSA010000178         5398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000178 DAJTSA010000000
VERSION     DAJTSA010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.179"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..497
                     /locus_tag="OO369_003955"
     CDS             <1..497
                     /locus_tag="OO369_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HCR5984029.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="OO369_003956"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="OO369_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="OO369_003957"
     CDS             2500..3114
                     /locus_tag="OO369_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR5984030.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="OO369_003958"
     CDS             3114..3443
                     /locus_tag="OO369_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR5984031.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="OO369_003959"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="OO369_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR5984032.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4529..4840
                     /locus_tag="OO369_003960"
     CDS             4529..4840
                     /locus_tag="OO369_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5984033.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4975..5373
                     /locus_tag="OO369_003961"
     CDS             4975..5373
                     /locus_tag="OO369_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5984034.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1177 a         1344 c         1425 g         1452 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtggcgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 ttttattgtc tgagatcaga gatacggcta ataaccgtat ctcttgacag agtgagcaga
     4441 tgcacataaa ttaatcatac ggttgatagc cgtattatgt atgagatcga ctccctacga
     4501 tttcattcag ttaatcaaag aagtcataat gcctggaaaa acagccgctc tatacgatgt
     4561 cgataaaacg ctgaaaaatg cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa
     4621 acttcgtgaa gctgcgcaag ccgttggggt agatttaagc gcttttattt tgagcgctgc
     4681 tatggaacgc gccgaaagtg tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag
     4741 ctgggaactg atgaaccaac tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc
     4801 gttaatgaaa aggaaaaaca gcgatggtcg acaagcatga agagattact ctgcccatag
     4861 tcctctcctg taattatcag tctgatatta cttatcctgg gcaaaaacag tttgattgcg
     4921 gtaaccctgt tatcgataaa tttgtacgcg ctacgcgcat cgctaaagaa aagtgtgcgt
     4981 aatagcgact gtgcggctaa agcacttatt gacagacaaa gtggtgaact gattggcatc
     5041 tgtactttta cggcatattc gctggaaaga caacgcgttt ctggcgtcct tcagggttca
     5101 caaccttcag aaattggtgt tgtcagatta gtcatgttgg ggggagcgcg gaagtatcaa
     5161 aagcggggct ttggtcagga cctactatgt gatttttttg aacatgtaaa aaaaattcac
     5221 ccggcattac caattaaagg ggtttatctt gatgctgacc ctgccgccat taacttttat
     5281 gctcgtctcg gctttgttca gctttcagcg acaccaaatg cttttggtgc tgtacctatg
     5341 tttttggcga ttcagcatat tctcgcggct tagattatta tcgccacccg ccagggca
//
LOCUS       DAJTSA010000179         5339 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000179 DAJTSA010000000
VERSION     DAJTSA010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.180"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="OO369_003962"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="OO369_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR5984035.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="OO369_003963"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="OO369_003963"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR5984036.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="pgeF"
                     /locus_tag="OO369_003964"
     CDS             1884..2615
                     /gene="pgeF"
                     /locus_tag="OO369_003964"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR5984037.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="OO369_003965"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="OO369_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR5984038.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1290 c         1457 g         1282 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 tgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcaac aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcggg
//
LOCUS       DAJTSA010000180         5181 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000180 DAJTSA010000000
VERSION     DAJTSA010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5181
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.181"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..746
                     /locus_tag="OO369_003966"
     CDS             <1..746
                     /locus_tag="OO369_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5984039.1"
                     /translation="VDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWN
                     NVTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIAT
                     LGSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKL
                     SFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPL
                     GLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1080..3359
                     /gene="ydeP"
                     /locus_tag="OO369_003967"
     CDS             1080..3359
                     /gene="ydeP"
                     /locus_tag="OO369_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR5984040.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3607..3810
                     /gene="safA"
                     /locus_tag="OO369_003968"
     CDS             3607..3810
                     /gene="safA"
                     /locus_tag="OO369_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR5984041.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3878..4639
                     /gene="ydeO"
                     /locus_tag="OO369_003969"
     CDS             3878..4639
                     /gene="ydeO"
                     /locus_tag="OO369_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR5984042.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            5041..>5181
                     /locus_tag="OO369_003970"
     CDS             5041..>5181
                     /locus_tag="OO369_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5984043.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDF"
BASE COUNT         1480 a         1102 c         1156 g         1443 t
ORIGIN      
        1 ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg
       61 atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat
      121 cgctgtactg gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg
      181 atattggtga taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg
      241 cggccggtgg agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa
      301 ttgcaacatt aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca
      361 gtgttgttat gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc
      421 cggactttcc tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc
      481 tctcatttta tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag
      541 cacctgatgc aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac
      601 tggctacggg agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt
      661 taagcgcaac ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca
      721 ttggtgtcac ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc
      781 acgaaaattg cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc
      841 cgcgcacaat tgaagccaga gtcttatttt ttatattgga aaatatgtta atttaaacac
      901 tatgagagat gatgcttaac ttatcgtatt taatctattg tttaacgata atagattaaa
      961 aatagaaaga gagtagtccc ttactcttat atatccatgt tggcgataat ccctttgtat
     1021 gtactgtgca tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga
     1081 tgaagaaaaa aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg
     1141 tagcgaatgc agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca
     1201 tgaataagcc agagggcttt gactgtccgg gttgtgcatg gccagatcct aagaacagtg
     1261 cgtcattcga catttgtgaa aacggcgcaa aagcaatcgc ttgggaagtc acggataagc
     1321 aggtaaatgc ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc
     1381 acgagcttga ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact
     1441 gttacaagcc attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct
     1501 atagtgatcc caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct
     1561 ttctttatca gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca
     1621 tgtgccatga accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg
     1681 tgttgctgga agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta
     1741 caaaccaccc tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga
     1801 tcgccatcaa tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt
     1861 ttgaaatgct gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg
     1921 gtggcgatat ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg
     1981 caagtgccgc aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg
     2041 gctttgacga gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt
     2101 ctggactaag tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca
     2161 ccattatctg ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc
     2221 aactggtcaa tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc
     2281 cactacgtgg tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt
     2341 ctgcagagtt tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg
     2401 gacatgctgc aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct
     2461 gcatgggggg caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa
     2521 cgcaattaga tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg
     2581 cacggcatag ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg
     2641 gtgcgcaggc ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt
     2701 taaaacccgc cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg
     2761 cagcactacc ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc
     2821 gcaatgacat tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc
     2881 ccggtggttt tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta
     2941 aggctaattt cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta
     3001 agctggtcat ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg
     3061 atgatcgcta tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag
     3121 ctaaaatttg ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg
     3181 gtaagcgcag ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc
     3241 gctcactggt gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc
     3301 cattaagtgg tattcctggc tataaaagta ttccggttga attagaacca tcaaattaat
     3361 gcctcttctc atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta
     3421 ttgcgcaatt tgctcacatg gattaaatta aactacatac tataagatat aaacttctgc
     3481 ctacagctgt aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc
     3541 agcctgttat attaagcata ctgattaacg acttttaacg ttatccgcta aataaacata
     3601 tttgaaatgc atgcgaccac agtgaaaaac aaaatcacgc aaagagataa ctataaagaa
     3661 atcatgtctg taattgtggt tgtcttatta ctgacactta cgttgatagc cattttttcg
     3721 gcaattgatc agctgagtat ttcagaaatg ggtcgcattg caagagatct tacacatttc
     3781 attatcaata gtttgcaaga ctggaaataa acagcaaatt ataaatgtga aatgtttaaa
     3841 aggtatcgat aaaactttat tgttttaagg agataaaatg tcgctcgttt gttctgttat
     3901 atttattcat catgctttca acgctaacat tttagataaa gattacgcct tctccgacgg
     3961 cgagatcctg atggtagata acgctgttcg tacacatttt gaaccttatg agcggcattt
     4021 taaagaaatc ggatttaatg aaaataccat taaaaaatat ctacaatgca ctaacatcca
     4081 gacagtgacg gtgcctgttc ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt
     4141 gcttaatgaa atgattgctt atctcaactc ggaagaacgc aatcatcata atttttcaga
     4201 acttttgctt ttttcttgcc tgtctatttt tgccgcatgc aaaggtttca ttacactatt
     4261 aactaacggt gtgctatccg tttccgggaa agtgagaaat attgtcaaca tgaagctggc
     4321 gcacccatgg aagctgaaag atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa
     4381 gaaaaaacta aagcaagagc aaacgacatt ctcacagatt ctattagatg ccagaatgca
     4441 gcacgcaaaa aatttgatac gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg
     4501 ttatgccagt acatcttatt ttatttatgc gttccgcaaa catttcggca acagtccgaa
     4561 gagagtttct aaggagtacc gttgtcaaag gcacacgggt atgaatacgg gcaacacgat
     4621 gagtgcttta gctatttgat tatttgctaa cgagtagtca accacgcacg ctacgtaaga
     4681 attatatggg gcagccattc cctgccccgc gttgttttta ggcgatatat ttattgaaat
     4741 aaataagtga catccatcac atatttatgc acttgcataa cctgttgcat gattatttat
     4801 gatctcaatt cggcattttg tcagtaaaat gcaataattt attaaatatc aataaattag
     4861 ttgtttatcg gcgagaaatt acttaataga acagaaagta atgtcaacgc tttatggact
     4921 gttatttccc tttttttagc taaatctgct atctctttat gtgactaact tcacttacat
     4981 ccacttattt ctcttcgtaa aattacttcg gaattaagta caataagaag aggaacattt
     5041 atgaagtctg cattaaagaa aagtgtcgta agtacctcga tatctttgat actggcatct
     5101 ggtatggctg catttgctgc tcatgcggca gatgatgtaa agctgaaagc aaccaaaaca
     5161 aacgttgctt tctcagactt t
//
LOCUS       DAJTSA010000181         5162 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000181 DAJTSA010000000
VERSION     DAJTSA010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.182"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..220
                     /locus_tag="OO369_003971"
     CDS             <1..220
                     /locus_tag="OO369_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5984044.1"
                     /translation="LYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHW
                     GFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(392..721)
                     /locus_tag="OO369_003972"
     CDS             complement(392..721)
                     /locus_tag="OO369_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR5984045.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(726..911)
                     /locus_tag="OO369_003973"
     CDS             complement(726..911)
                     /locus_tag="OO369_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR5984046.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(908..3547)
                     /locus_tag="OO369_003974"
     CDS             complement(908..3547)
                     /locus_tag="OO369_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR5984047.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3755..4744
                     /gene="ldhA"
                     /locus_tag="OO369_003975"
     CDS             3755..4744
                     /gene="ldhA"
                     /locus_tag="OO369_003975"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5984048.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4855..>5162
                     /gene="hslJ"
                     /locus_tag="OO369_003976"
     CDS             4855..>5162
                     /gene="hslJ"
                     /locus_tag="OO369_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR5984049.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     "
BASE COUNT         1307 a         1322 c         1203 g         1330 t
ORIGIN      
        1 tttgtaccga atgtttgccg ataaaggttt ggtagtcgcg caatatattc gtaaccgtcg
       61 tctcgatttt tgtgcagatg cgattcgcca tgccgcagat gatgaaaaac tggcaggcat
      121 cggctttcat tggggatttt ctgaccagag tcatttttca acggtattta agcaacgctt
      181 tgggatgacg ccaggcgagt atcgacgtaa attccgctaa ctaattggcg ttgcatgtac
      241 tgcaacgcca attagttaaa acagatattt gactatgcat ttttcagggc gaaaggcaat
      301 ttgacaggag tttttgaata ttcaccttgt ttcaggaaaa tagatcaatc gctttctcct
      361 gtagcgttat cccgatattg cggttttgct attaaaactt tcgcacccat ttaccgttta
      421 tcccttgtac atattccccc ggtttggccc gagctacgag cttttgcccg gcgagtttcg
      481 cgatatcatc tacagacaca ttattttgct ttgcccgttg ttggtaactc gctttacgtt
      541 cggcattaat ttcggttact aatttctctg tttcagcatc cgttttcagc gcaaccagat
      601 aaccataaaa tgtttcacct acccgcccct gagttctggc ttcatccaga gtcaatgcca
      661 ttacattgct gcttaccagc ccaacaagaa acgcacaaag aagtaatgtt ttcttcatca
      721 tcgcctcaga aaagatcgct acgagtttca agcagctctt cgacatcttt gtctgccttg
      781 atgatgatct catgctcaat tttaacgttc atattgatag tgatcggttc cttaggtgca
      841 gcgacttcaa tgcgaggcgt acagccaacc agcataaaag atgacgtcaa cgcagccagt
      901 aaaattttca ttgtttttcc tcacactctt ttccttgcgg acagtcattt cccggcagac
      961 gtgcgttctg ctccaaccat gcctggagat tatcgccaaa gcgtaaactg cgccacagcg
     1021 taaaaatatt ctcttcatga tggtaattaa ggtttaccgt accgctttta ccatcaacgc
     1081 gactggtacc tgtaatgttg gcctgcatgg ttaataaacc gagattatct aagttaattt
     1141 ttgtcgatga ccggctaatt tccatatagc gcaaccagtt aattgctgaa cccgcagtca
     1201 tattgtcttt caccaccgca tctgcggtat ctttatccag ccgcaaggtc attagcccgc
     1261 tatttgccag ccaaccatct ttcactatcc atttttcgtt gtttagccat aacggcaatg
     1321 caccactaaa tgccccggac atggcgaatt gtttcggatt gacggcgcta accagttcgc
     1381 tggatgagag attattcagc cgcaacagcg ccgggtcatg ttgcggcata cgtaattgtt
     1441 tcatcagcac gttaccgcct aacacatcga cgctaacatc agtcaacagc aagggttctt
     1501 cttcagtcca cggataacgc ccttgcaaat cagccgtaat atttttcgct gtcaccagat
     1561 taatcacttc ggcaattcgc aacgtaacgg ggccgcgagt ccccagatgc caggctccat
     1621 cggcaaaacg gaaaggcagg acaaaatcga caccgttaac ctgattatct ggcatccagg
     1681 cactaccgcc tttcaacacg ccgtgccctc ccgcgcggaa tccttgttca ggcgcagcag
     1741 aaaatgcaac ctgagcatac agttcaccat cgcgtaagtt catcttccag tcgggtggca
     1801 ccagcggctg gaatacagtc agtgattgtt ttggccacca ggcgttaccg cgcagacgaa
     1861 taccgtccca gcgaccattt acccgaaccg ggccaatctc accagcatgt aaatcgcctt
     1921 taaagaggaa ataggtcgga ttgcgcccat cgacgctaaa ttttaaggtt gatggcggta
     1981 acacactgcc gccagtgaac agcgtttgcc cggcgtccag tgacagcgcg ccgctgaagg
     2041 agggatgctg tgcgtcacgt acccagacga cgggcttgtc gagaattaat cgcggctttt
     2101 ctaccgtcat cgtaccgtat tgtaactggt cgaaaccggt ggagagatcg gtcagcgtaa
     2161 tcgtgctgtc atgccactca ccttttcctg cgacatccca ggtggcattc atcggtgtaa
     2221 aactcccttt tccccagtag cgccactgcc agcggccagc gtcagggaga aaatcattcg
     2281 ccagcccatc catatgcagc acgaaatcgc ccagttcatt ttcatgcgcc tgcaaaatgg
     2341 cctgcaaacg tccgtcaaca ccacgttggg tgacttttac acccgctaaa ggccagcgga
     2401 tttcatcgat gtccagcgaa tcgatgactc ttccctttga acgaagtaac gcgcctggct
     2461 caaaggtcag cgttgggtca gacagacttc cacttagctg cgcaggtaaa cgggcatata
     2521 aaatgaggtc cgcctgtttc gcttcaccgg tcagctgcag aggcagttga ctgttatcca
     2581 tgcttaattt tcctgggcca aaattaagca ccgcgttgcc cttacccgct tgcccctggg
     2641 tcagcacatt cagtcgtccg ctgaccagag cgttctcaag ccctgcctgc cagttgtcga
     2701 ctttgacacc cagtcggcca ctcaaaggaa aaccagcata cagccagctc cagcgaccat
     2761 cgcttacggt caattgttga cgagtaattt gccacggcaa atcgagcaac ggatcgccgt
     2821 tatcccgtgc cagcacgatc aattgcccgc tattttcctg ccagtccagc tcggcatcca
     2881 ccagtgacgg ttcctgcggc aagtttaacg tcgcagtagc atgcccactt acaggaagtc
     2941 catccggcac gagcggcata gtaaattccc ccaccagttt aaccggcggc tgattttcaa
     3001 acgcgacgac atccagttcg ctgactgtaa gttgttgccc tttcagctgg ccttgaaatt
     3061 taactttttc gccctgataa cgcagttgct ggatatcaga ggttaatgcg agagagagtt
     3121 ttccctgcca ttcctgccag ggagaaaaaa tcagtttatc gatattgatc caggtgttag
     3181 gcagcatgga ctgccactgc gcgagggttt ttggagcagc tggcgattgc tccgtctgcg
     3241 gcaatttcgc cagacaagca gaatcaagtt ctaccgtgcc gacgttcaat aaccagcggc
     3301 tgggatgtga aaggctggcg ttggtgatat gcgcaagctg acaatctccc accagataac
     3361 ggagatcggg aatgattaaa cctttacgcg taatgcgtgg gctttcatct aatgcaatac
     3421 gtgtcccgag cggtagccag atgtccgcca gcgtgggaac ccacagcccg agcgtcatca
     3481 gcagcgtcaa cggcacaaga ataatcagta ataacagcgc gagaacggct ttatatttac
     3541 ccagcatggg tagttaatat cctgatttag cgaaaaatta agcattcaat acgggtattg
     3601 tggcatgttt aaccgttcag ttgaaggttg cgcctacact aagcatagtt gttgatgaat
     3661 ttttcaatat cgccatggct ttcaattata tttgaaattt tgtaaaatat ttttagtagc
     3721 ttaaatgtga ttcaacatca ctggagaaag tcttatgaaa ctcgccgttt atagcacaaa
     3781 acagtacgac aagaagtacc tgcaacaggt gaacgagtcc tttggctttg agctggaatt
     3841 ttttgacttt ctgctgacgg aaaaaaccgc taaaactgcc aatggctgcg aagcggtatg
     3901 tattttcgta aacgatgacg gcagccaccc ggtgctggaa gagctgaaaa agcacggcgt
     3961 taaatatatc gccctgcgct gtgccggttt caataacgtc gaccttgatg cggcaaaaga
     4021 actggggctg aaagtagtcc gtgttccagc ctatgatcca gaggccgttg ctgaacacgc
     4081 catcggtatg atgatgacgc tgaaccgccg tattcaccgc gcgtatcagc gtacccgtga
     4141 cgctaacttc tctctggaag gtctgaccgg ctttactatg tatggcaaaa cggcaggcgt
     4201 tatcggtacc ggtaaaatcg gtgtggcgat gctgcgcatt ctgaaaggtt ttggtatgcg
     4261 tctgctggcg ttcgatccgt atccaagtgc agcggcgctg gaactcggtg tggagtatgt
     4321 cgatctgcca accctgttct ctgaatcaga cgttatctct ctgcactgcc cgctgacacc
     4381 ggaaaactac catctgttga acgaagccgc cttcgatcag atgaaaaatg gcgtgatgat
     4441 cgtcaatacc agtcgcggtg cattgattga ttctcaggcg gcaattgaag cgctgaaaaa
     4501 tcagaaaatt ggttcgttgg gtatggacgt ctatgagaac gaacgcgatc tgttctttga
     4561 agataaatcc aacgacgtga tccaggatga cgtattccgt cgcctgtctg cctgccacaa
     4621 cgtgctgttt accgggcacc aggcattcct gacagcagaa gctctgacca gtatttctca
     4681 gactacgctg caaaacttaa gcaatctgga aaaaggtgaa acctgcccga acgaactggt
     4741 ttaatcttgc agctcccctg caatccaggg gagctgattc agataatccc caatgacctt
     4801 tcattctcta ttcttaaaat agtcctgagt cagaaactgt aattgagaac cacaatgaag
     4861 aaagtagccg cgtttgttgc gctaagcctg ctgatggcgg gatgtgtaag taatgacaaa
     4921 attgctgtta cgccagaaca gctacagcat catcgctttg tgctggaaag cgtaaacggt
     4981 aagcccgtga ccagcgataa aaatccgcca gaaatcagct ttggtgaaaa aatgatgatt
     5041 tccggcagca tgtgtaaccg ctttagcggt gaaggcaaac tgtctaatgg tgaactgaca
     5101 gccaaagggc tggcaatgac ccgtatgatg tgcgctaacc cgcagcttaa tgaactcgat
     5161 aa
//
LOCUS       DAJTSA010000182         5151 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000182 DAJTSA010000000
VERSION     DAJTSA010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.183"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            190..447
                     /locus_tag="OO369_003977"
                     /pseudo
     CDS             190..447
                     /locus_tag="OO369_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(497..1447)
                     /gene="uspE"
                     /locus_tag="OO369_003978"
     CDS             complement(497..1447)
                     /gene="uspE"
                     /locus_tag="OO369_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR5984050.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="OO369_003979"
     CDS             complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="OO369_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR5984051.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="OO369_003980"
     CDS             complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="OO369_003980"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR5984052.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3072..3215)
                     /locus_tag="OO369_003981"
                     /pseudo
     CDS             complement(3072..3215)
                     /locus_tag="OO369_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3605..4420)
                     /locus_tag="OO369_003982"
     CDS             complement(3605..4420)
                     /locus_tag="OO369_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5984053.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4517..>5151
                     /locus_tag="OO369_003983"
     CDS             4517..>5151
                     /locus_tag="OO369_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5984054.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT         1374 a         1174 c         1226 g         1377 t
ORIGIN      
        1 gctattttac cgcgacaatt cattcagaac atcaatagtc agggaaggaa gtagcaacat
       61 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      121 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      181 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      241 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      301 atagaacagt acaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      361 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      421 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      481 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      541 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      601 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      661 cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg tagacctttt tctacgtgcg
      721 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      781 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      841 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      901 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      961 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
     1021 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1081 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1141 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1201 cagcattgag ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc
     1261 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1321 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1381 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1441 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1501 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1561 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatgg
     1621 ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1681 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1741 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1801 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1861 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1921 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1981 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2041 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2101 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2161 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2221 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2281 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2341 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2401 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2461 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2521 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2581 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2641 gatggcaagg tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg
     2701 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg
     2761 ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg
     2821 gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac
     2881 cacctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     2941 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     3001 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3061 tccgttctct cttaagacaa acgtgggtaa ataccctgac ctatcggcag accgacaaga
     3121 taccacgcca gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact
     3181 aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga
     3241 ttttttaatt tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga
     3301 taattccagt cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata
     3361 gccgccagac accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt
     3421 tacagagcta aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt
     3481 acacacagca gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt
     3541 gttataacgg ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata
     3601 taagttaaat catttgtctt gtgattgcat aattatgcat cgttaatcga cgagtaaatt
     3661 taccgggcga gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat
     3721 agcccagacg atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag
     3781 aaataacgtg catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata
     3841 agcgcatgag tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca
     3901 gatacccttg gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc
     3961 aagggagttc aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga
     4021 gggctgaaaa acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa
     4081 ttgcaattag taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca
     4141 gcgtttcact ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa
     4201 caaacttatg cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga
     4261 cgctttctcc agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga
     4321 gatgtttatg ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt
     4381 gatgccctgg tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca
     4441 gaaatcatca aataagccat gttaaatttt aaatatgacg tatgcaagtt tccggatata
     4501 tacggtgtaa agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg
     4561 tattcttttg actggcgttg tcttttttat ctggagggta agcaaaggaa gatttataca
     4621 gacgacggat gatgcttaca ttggtggtaa tatcaccacg gtggcaagta aagtttctgg
     4681 ttatatttct gctatagagg tgcgcgataa tcagagcgta aaaaaaggcg atattatcct
     4741 gcgtctcgat gatcgggatt atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag
     4801 tagcaaagcc aatctcgaag gcattcaagc cactattacg atgcagcagt cgataattca
     4861 aagtgccagt gaaacatggc aggcagtaaa acatgaggag caaaagcgcc tgcgagatac
     4921 tgaacgttat gaaaaactcg cacaatcagc ggcaatatcg caacaaatta ttgataacgc
     4981 caggtttgac taccagcagg tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt
     5041 ggagaagcaa cggctggcag ttttatctgc tcaggaagaa aacgtacgtg cgagtatcga
     5101 agaagttcag gccgctttga ctcaggcact actggatctg gaatataccc t
//
LOCUS       DAJTSA010000183         5010 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000183 DAJTSA010000000
VERSION     DAJTSA010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.184"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(674..1090)
                     /locus_tag="OO369_003984"
     CDS             complement(674..1090)
                     /locus_tag="OO369_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984055.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1117..1257
                     /locus_tag="OO369_003985"
                     /pseudo
     CDS             1117..1257
                     /locus_tag="OO369_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1257..2282
                     /locus_tag="OO369_003986"
     CDS             1257..2282
                     /locus_tag="OO369_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5984056.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(2335..2424)
                     /locus_tag="OO369_003987"
     tRNA            complement(2335..2424)
                     /locus_tag="OO369_003987"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2388..2390),aa:Ser,seq:cga)
     gene            2518..3315
                     /gene="mtfA"
                     /locus_tag="OO369_003988"
     CDS             2518..3315
                     /gene="mtfA"
                     /locus_tag="OO369_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR5984057.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3416..3491
                     /locus_tag="OO369_003989"
     tRNA            3416..3491
                     /locus_tag="OO369_003989"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3449..3451,aa:Asn,seq:gtt)
     gene            3653..4915
                     /locus_tag="OO369_003990"
                     /pseudo
     CDS             3653..4915
                     /locus_tag="OO369_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1384 a         1182 c         1120 g         1324 t
ORIGIN      
        1 acatcgcctt ttgctgcttc atagcctgct tctccttgcc tttcggcacg taagaggcta
       61 acctacatgt gttcagcatg gattgagcct cagattaatg ttaagcgtct tgcaggacgc
      121 gtaatgttaa ctggggcttt tctctatctg ccttttggtg ttcatgcctg agacagatag
      181 cctcaagcac ccgcagtcat tctacttaac taagatttcc ctgcaaaccg tttttgtccg
      241 gcacagtaaa tatccaacta aaccaatagc gttcgctgta tttaccgcca gtattcaatg
      301 cacatgaccg ccatgaacac ccctaaaaaa agggcattta tatgtccaaa cattaatatc
      361 aaaacatcaa ttttttccat ataccttgct gtgaagatga tgggcataca tgatgcgaac
      421 aaccagaacg caacaaacaa aaactgcaat gcgtttttca ttattccccc tacaatcaat
      481 gtgcaataac atttaaacac acctcaattt ggccggacat ataaatatct aaaccagaaa
      541 aaatcactta catagcgtta caaactcttt agtctaaata ctcatcgtaa aacattcccc
      601 atacttatca gtccgttccg cgccaggtag cttattgcct tatctggcaa cctataatca
      661 ggtttccgct ttttcagttg gctggtcgtt taaccgacat agttaaccca ttaatctggt
      721 tgccggatgc tggtggattt tcgcgtttta attgttcata aaagtgcaca gctttaacca
      781 gttcttctga tgtaaccggg actggtgggg cagtgaataa ggcctgaatt tcatagttcg
      841 gcctgtcgtt acaatcctct tttttcggta catatttcca gtcaccaacc cacaactccc
      901 cctgagagtc cataacacct tttttcacgt agcgatatcg ccacgctatc ggctctgctt
      961 ccagcgatgc ccgtgcaatt ttgaataact cgccctctac tcgcgccatc cctgaattgg
     1021 ggtggcattt cgtaatcgct atttttaatt tggcttcttc gattaattgt tcttttgtta
     1081 attcagtcat tttcattacc gccctggtaa tgctgccacc agcgaaaatg gttaatggaa
     1141 aacgggcttc cgttctatac caatcgtagt ggcaaaccga ttgtcagccg ggatctattt
     1201 acctgcaata aaactttacc accacgcgag gtagagccga attttggtgc gatctgatgg
     1261 gaagacgaag gaaaaatcct gaacacgaaa aattacctcc aaatgtatac ccaaataaat
     1321 atagttatgt atggaaacca acatccagag aatctgtaac actaaccgcc atcaaggatg
     1381 gtttagctgc tttatggaaa aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga
     1441 cattcggtcg cttgtgggaa aaattcctcg ccagcgccta ttacagtgac ctcagtccaa
     1501 gaacacaaaa agattatctg caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc
     1561 cggcagattc cataaaacca gaacacatcc gtcgatacat ggacaagaga ggggaacaga
     1621 gtaaaacgca agccaaccat gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg
     1681 agcgagggta cgtgaaggct aacccatgtg caggtgtaag taaattcaag gccaaaaacc
     1741 gcgaacgata tgtaaccgac aaagaatacc aggcagtatt aagcgttgca cctcttcctg
     1801 tttttatcgc aatggaaatt gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc
     1861 tgaaatggga gcagattgga aacgacggga tctttatcca gcaagggaaa acaggaaaaa
     1921 aacagataaa agcatggagt ccacgattac aggcggcgat cgaaaaagca aaacagttac
     1981 caacatccgc ctatgtaatc agcaatcaat acggcaaccg atatatgtac aaaggcttta
     2041 acgaaatgtg ggtagaagca agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact
     2101 tcacctttca tgatctgaag gcgaaaggaa tttcagacta tgaaggaagc agtcgggata
     2161 agcaactttt ctctggtcac aaaaccgagg ggcaagtgct aatctatgac aggaaggtta
     2221 aagtttcacc gacacttgat gtcccgttac ctgaaaatat tccaagaaaa tattccaagt
     2281 aattccaagt gtgatttttg tcactgactt aatgatgtat aagtgattga attttggcgg
     2341 agagaggggg atttgaaccc ccggtggagt tgcccccact ccggttttcg agaccggtcc
     2401 gttcagccgc tccggcatct ctccgttcag atggttgcca tgatgccagg aaatttggca
     2461 ttttaacagt ccctgtccgt gcaattttgt tcaagtgacg agtttgcgag caaaacgatg
     2521 attaagtggc cctggaaagt acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa
     2581 gcactatcga tccccctttt aacgtgtctg acagaacagg aacaaagcaa attggtcgct
     2641 cttgccgaac gttttttaca gcaaaaacgg cttgttcctt tacagggctt tgagctgaat
     2701 tcattaagaa gctgccggat agcacttcta ttttgcctgc ccgttctgga gttaggactg
     2761 gaatggctgg atggttttca tgaagtctta atttatcctg cgccatttgt ggtcgatgat
     2821 gaatgggaag acgatatcgg tctggtgcat aaccaacgta ttgttcagtc aggtcagagc
     2881 tggcagcaag ggcctatcgt tttgaactgg ttggatatac aagattcttt tgatgcttct
     2941 ggttttaacc tgattattca tgaagtcgct cataagctgg acacccgtaa cggcgatcgc
     3001 gccagcggag ttccctttat ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat
     3061 gctgcaatga acaacattca ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt
     3121 gatgcttatg ctgccagtga tcctgctgaa tgttttgccg tactttctga atatttcttt
     3181 agcgccccag aactttttgc tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt
     3241 tatcaacaag atcctttgca gagactgcat cacgctaatg atacagactc gttttcagcg
     3301 acgaatgttc attaattaac aactttgaag attaattaac caattgaaat ggcttatgaa
     3361 atttagtgtt gacagacaag gtaccgctaa gtaatatgcg ccccgttctc acgattcctc
     3421 tgtagttcag tcggtagaac ggcggactgt taatccgtat gtcactggtt cgagtccagt
     3481 cagaggagcc aattttctgt tttcatgcat ccttgcgaat ctttatctga tgttgattca
     3541 acaggttagt gtgaaaactc ttctcggtgc attttgattt taccctacgc atcgggaaaa
     3601 attggtggtc aaatctggga tcaggttagt tcgataatgg agtgaccccc atatatccct
     3661 taccgacgca aaaatccgca ccctcaagcc ttctgataaa ccctttaaag tctccgattc
     3721 tcacggtctg tatctgctgg tcaagccggg tggctcccgc cactggtatc tcaaataccg
     3781 tattagcggt aaagaatccc gcattgcgct gggtgcctat ccagccatct ccctgtctga
     3841 tgcgcgacag caacgtgaag gtatccgtaa aatgctggcg ctgaatatca acccggtaca
     3901 gcagcgggct gctgaacgtg gctcacgaac accggagaaa gtttttaaaa acatggcgct
     3961 ggcgtgacat aaaagtaaca ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag
     4021 cctgaacaat cacatctttc cggtcatcgg gaacctacct gtatcagaac ttaaaccccg
     4081 tcatttcatt gacctgctga aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg
     4141 cacacggcag cacctgagta acataatgcg ccatgcggtc catcaggagt taatcgatac
     4201 gaaccctgca gcaaaccttg gcggcgtgac cacacctcct gtcagacggc actatcctgc
     4261 cctgccgctg gagcggctgc ctgaactgct tgaacgtatt ggggcatatc atcagggccg
     4321 tgaactgacc cggcatgccg ttctgctgat gctgcatgtg ttcattcgct ccagtgaact
     4381 gcgtttcgcc cgctggtcag agattgattt cacaaaccga gtctggacga tacccgcgac
     4441 gcgagaaccc attattggcg tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca
     4501 tatcgtcccc ctctcagaac agtccatcgc cattctgaaa cagattaagg atatcaccgg
     4561 taataatgaa ctgatcttcc ccggcgacca taacccgtat aagccaatgt gtgaaaacac
     4621 ggtcaataag gcactgcggg tgatgggtta cgacacgaaa aaggatatct gcggtcacgg
     4681 cttccgggca atggcatgca gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt
     4741 agaacgccag atgagtcatc aggagcgcaa taccgtgcgc atggcttata ttcataaggc
     4801 agagcacctg gaagcccgca aagcgatgat gcagtggtgg tcggattatc tggaagcatg
     4861 ccgagaatct tatgcaccgc cttatacaat tggtaaaaat aagtttatcc catagtatac
     4921 taacgaatcc cacactctgg caaattacca ccacctctac aggaggtggt tctatataga
     4981 cttttatcaa cttcatgacg aaaaacacct
//
LOCUS       DAJTSA010000184         4973 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000184 DAJTSA010000000
VERSION     DAJTSA010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.185"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="OO369_003991"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="OO369_003991"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR5984058.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="OO369_003992"
     CDS             1020..1703
                     /locus_tag="OO369_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCR5984059.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="OO369_003993"
     CDS             1700..2161
                     /locus_tag="OO369_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR5984060.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="OO369_003994"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="OO369_003994"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5984061.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="OO369_003995"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="OO369_003995"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5984062.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="OO369_003996"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="OO369_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR5984063.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="OO369_003997"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="OO369_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR5984064.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1176 a         1305 c         1239 g         1253 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg cggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaaa cgctagtata ttctgaatat tccgaatagt tac
//
LOCUS       DAJTSA010000185         4866 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000185 DAJTSA010000000
VERSION     DAJTSA010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.186"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..527
                     /locus_tag="OO369_003998"
     CDS             <1..527
                     /locus_tag="OO369_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR5984065.1"
                     /translation="TTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
     gene            724..2163
                     /gene="aldA"
                     /locus_tag="OO369_003999"
     CDS             724..2163
                     /gene="aldA"
                     /locus_tag="OO369_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR5984066.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2205..3205)
                     /gene="gap"
                     /locus_tag="OO369_004000"
                     /pseudo
     CDS             complement(2205..3205)
                     /gene="gap"
                     /locus_tag="OO369_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            3394..3924
                     /gene="cybB"
                     /locus_tag="OO369_004001"
     CDS             3394..3924
                     /gene="cybB"
                     /locus_tag="OO369_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR5984067.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4165..4338
                     /locus_tag="OO369_004002"
     CDS             4165..4338
                     /locus_tag="OO369_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984068.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(4410..4559)
                     /gene="hokB"
                     /locus_tag="OO369_004003"
     CDS             complement(4410..4559)
                     /gene="hokB"
                     /locus_tag="OO369_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCR5984069.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4660..4800)
                     /locus_tag="OO369_004004"
                     /pseudo
     CDS             complement(4660..4800)
                     /locus_tag="OO369_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1246 a         1118 c         1207 g         1295 t
ORIGIN      
        1 gcaccactgg cagagcagaa gcgaccatcc tggtggatat cgctcatcag ttctggcata
       61 ttccgcatga aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct
      121 ttagcatcac gctattgaat caggccgtag aacgagttca tacggctatc gttgtacagg
      181 accccaccat gcagcggcgc acgatggcaa cgttccgccg tatgaccggg gacaatcccg
      241 atgcaccacg ctggttaagt tatcccggat tcgttcctca gttaggaaat aacgcagaca
      301 gtgtaatctt tattaatcag ttacaaggat tatggccggt tgagcgttat ctctcactac
      361 tcactggcga gctgccgcgt ttacacgatg atagcgatgg ctacggtccc cgcgggcgag
      421 attttatcgt tcacgttgat tttccggcag aagtcatcca tgcatggcaa acgctgaaac
      481 atgatgcggt gctcatcgag gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt
      541 gtgaaccact tgtttgcaaa cgggcatgac tcctgacttt tatttctgcc ttttattcct
      601 tttacacttg tttttatgaa gccctccaca gacttgtcct ttcccgatgc cgtctctctg
      661 atgattgatg ttaattaaca atatattcac cgaaaacaaa catataaatc acaagagtcg
      721 cccatgtcag tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt
      781 ggagacgcat ggattgatgt ggtaaaccct gctacagagg ctgtcatttc ccgcataccc
      841 gatggtcagg ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa
      901 tgggaagcgt tgcctgctat tgaacgcgcc agttggttgt gcaaaatctc cgccgggatc
      961 cgcgaacgcg ccagtgaaat cagtgcgctg attgttgaag aagggggcaa gatccagcag
     1021 ctagctgaag tcgaagtggc ttttactgcc gactatatcg attacatggc ggagtgggca
     1081 cggcgttacg agggcgagat tattcaaagc gatcgtccgg gagaaaatat tcttctgttt
     1141 aaacgtgcgc ttggtgtgac taccggcatt ctgccgtgga actttccgtt cttcctcatt
     1201 gcccgcaaaa tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acccagtgaa
     1261 tttacgccaa acaatgcgat tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc
     1321 ggcgtgttta accttgtact ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac
     1381 ccaaaggtcg caatggtcag tatgacaggc agcgtctctg caggtgagaa gatcatggcg
     1441 actgcggcga aaaacatcac caaagtgtgt ctggaattgg gtggtaaagc accggctatc
     1501 gtaatggacg atgccgatct tgaactggca gtcaaagcca tcgttgattc acgcgtcatt
     1561 aatagtgggc aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaagg catttatgat
     1621 cagttcgtca atctgctggg tgaagcgttg caggcggttc aatttggtaa ccccgctgaa
     1681 cgcaacgaca ttgcgatggg gccgttgatt aacgccgcgg cgctggaaag ggttgagcaa
     1741 aaagtggcgc gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa agcggtagag
     1801 gggaaaggat attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt
     1861 atgcatgagg aaacctttgg cccggtgctg ccagttgtcg catttgacac gctggaagat
     1921 gctatctcaa tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat
     1981 ctgaacgtcg cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt
     2041 gaaaacttcg aagctatgca aggtttccac gccggatggc gtaaatccgg tattggcggc
     2101 gcagatggta aacatggcct gcatgaatat ctgcagaccc aggtggttta tttacagtct
     2161 taatgagtga aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat
     2221 ttttcgaggg tgcgaataag ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc
     2281 gttttcacca gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc atcaaacact
     2341 gaaccgaaat ggctgccaat gatatcggaa gagactattt cttcatcggt ataaccaaat
     2401 gactcgttat tggtggttgc ttgtttaagt gcgttattca cctcttcggc agtcactttt
     2461 ttccgagaat ggacaccagc tcagtgaccg aacctgtttt caccggcacg cgttgcgcat
     2521 gacctttcag tttgccgctc agttccggga tcaccagacc aatggctttt gccgcccccg
     2581 tagtgtgggg aatgatattt tctgccgctg cgcgtgaagc acgtaaatct ttaccacgcg
     2641 ggccatccac cagtgactgg gtgccagtat aggcatgaat ggtcgtcatc gtgccgactt
     2701 ctatcccgaa actgtcatgc aaggctttgg ccatcggcgc aaggcagtta gtggtgcatg
     2761 acgccacgga aacaatggtg tcgttgccat ccagagtgtc gtcattaacg ttataaacga
     2821 tggttttcat ttcaccggca ggagcggaaa tcaacacctt cttcgcacca gcatcaagat
     2881 gcgcctgcga tttatcggcg gaggtataaa agccagtaca ttcgacaatg atttctgccc
     2941 ctttcgcttt ccacggaata tttttagcct ctttttcggc gtaaaccgcg atacttttcc
     3001 catcaacgat aagtgaatct tccgtaaaat caacgctcca ggggaacgga ccatagtttg
     3061 aatcatgttt cagcagatag gcgagaattt ttggggaagt aagatcatta atagcgacaa
     3121 cgtctatgtt gcttttgact tcaagtaaac gacgcaacac cagtcgaccg atacgaccaa
     3181 aaccgttaat accaacttta ctcatggttt tctcctgtca ggaacgttcg gatgaaaatt
     3241 gatcctttcc aggcttagac caggattgtg ggatgggcaa tctccattct tacagtgaaa
     3301 cgtaacgtac tgaaaacggg tgaacaatat ttaatgaaat tttgagaaaa gcccgttatg
     3361 ttaacggaaa attatgttaa agcaggaaat gttatggaaa ataaatactc aaggttacaa
     3421 atcagcattc actggctggt ctttttgctg gttatcgcag cgtattgcgc aatggagttt
     3481 cgtggtttct tcccacgtag cgatcggccg ctcatcaaca tgattcatgt ttcctgtggc
     3541 atctcaattc tcgtgctgat ggtggttcgt cttttgttaa gactgaaata cccaaccccg
     3601 ccgattatac ctaagccgaa accgatgatg acgggactgg cgcatttggg acatttggtg
     3661 atttatctcc tgtttattgc gctgccagtg attggtttgg tgatgatgta taaccggggc
     3721 aacccgtggt ttgcgtttgg tttgacgatg ccttacgctt cagaggccaa tttcgaacgg
     3781 gttgatagct taaagtcgtg gcatgagacg ctggcgaatc tgggatattt tgtcatcggg
     3841 ttgcacgctg cggctgcact ggcacaccac tatttctgga aggacaacac acttctacgc
     3901 atgatgccgc gtaaacgttc ctgaaggata tttaaagaaa acgcctgtac taaaacccgt
     3961 ggtacaggcg aagaatacgg gtctacatcg gaagcgccta ttatatttat ttgtatgaca
     4021 aataaaacca taatccttgc ccatacgtcc atctggctta tttttaatca attcacccga
     4081 tctttgatct catcaacggt atcaaaataa accagcgtat aacgttcaaa tcgcacataa
     4141 gattcgataa accatggagg ttatatgaaa aaactggcac ttattttgtt tatgggaacg
     4201 cttgtttcct tttatgccga tgccgggcgc aaaccctgtt ctggttcgaa aggggggatc
     4261 tcacactgta cggcaggcgg caaatttgtc tgtaatgatg gttctattag tgcatcgaaa
     4321 aaaacatgca ctaactgaag tgtaaaaggg gtgccatgag aaaatgaatc tgctagtcag
     4381 atgcgcgggg aaaatccccg cgcttgccct tacctggacg tgcaggccat gagcgcagca
     4441 acctccttat caccgtcccg gaaccgcagt tcgtagagcg tttgtcgggt caggagtgtg
     4501 aatgccagaa tcgtaatgca gataatgagc agacacacca ccagagggtt gcgcttcata
     4561 gcctttctcc ttgccggatg gcgggtaaga ggctaacaat ctgaattgct tggttcattc
     4621 gttggcctcg gtttgtactg accccaaaaa gttggacagt taaacacgag gcatataggt
     4681 ctgattccga tattcatttg gagtcagacc ttttaatttc aggctaattc ttctgctgtt
     4741 gtagtattca atatattccg taacagcatc cttcagttcg cttatattac tgaactcatt
     4801 agactggccc cctgaatctc cagacaacca gtatcactta tttaagtgat agtcttaata
     4861 ctagtt
//
LOCUS       DAJTSA010000186         4809 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000186 DAJTSA010000000
VERSION     DAJTSA010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.187"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(293..1150)
                     /gene="sitD"
                     /locus_tag="OO369_004005"
     CDS             complement(293..1150)
                     /gene="sitD"
                     /locus_tag="OO369_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR5984070.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1147..2004)
                     /gene="sitC"
                     /locus_tag="OO369_004006"
     CDS             complement(1147..2004)
                     /gene="sitC"
                     /locus_tag="OO369_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR5984071.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2001..2828)
                     /gene="sitB"
                     /locus_tag="OO369_004007"
     CDS             complement(2001..2828)
                     /gene="sitB"
                     /locus_tag="OO369_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR5984072.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2828..3742)
                     /gene="sitA"
                     /locus_tag="OO369_004008"
     CDS             complement(2828..3742)
                     /gene="sitA"
                     /locus_tag="OO369_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR5984073.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4174..>4809)
                     /locus_tag="OO369_004009"
     CDS             complement(4174..>4809)
                     /locus_tag="OO369_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5984074.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVALLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1274 a         1175 c         1137 g         1223 t
ORIGIN      
        1 acgatgagat ttatcgccgt cagcgggagc tgaaggaaga gctgaatagt ctgaaggatt
       61 tgaattcggt taggaatttt atcgtggaat aacagaagct gcggcacgtc gtatgcagga
      121 acgtgccgcg gttggctggt aaactttcga tagtgcgagt attgaatgat ttctaggcgt
      181 tatcgatttt acgtattttt tgcatgatag aattcgtacc tcctcccaac gaccttccat
      241 ggctttccgg acttctgtag gctggcggac gatgctgtcc tcggatgttg tgctaattgg
      301 gtgataacag atcctgtgta tgggcgtttc cctttgtgcg tgcgttgatg ctggtaacgg
      361 taaacgtcat gatgaacacg accgcgaata agacgacaat cgtcggagct ggtgcgctgt
      421 cgataaaaaa ggacagccag acaccagtca ttgacactat taccgatatg ccagtcgcca
      481 gtaggagcgc aatatgaaag cgttgtgtca ggagtactgc aatggcgcca ggcgcaatga
      541 gcagagaaat agagagaatg atacctactg ctttcagcgt cgccacaatg gtcagggaaa
      601 ccatgcacag cagcccataa tgcagccatc tcgtatgcaa accgcttacc tgcgcctgtt
      661 gataatcaaa gctgaacagc aaaaaatccc gccattttac gctaataaca agtgtaacca
      721 gcccggcaat aatcaccgtc tggattatgt cgccgatggt tatcccgagc atgtcgccga
      781 aaagaatatg gtcaagatgt acgtctggtt taattgcaat atacaagatc agacctgcgg
      841 caaacatgcc agaaaaaaca atccccataa ctgtatcttg cttgattcgg ctgttgtctt
      901 tcaggtatcc cgtcgccacc gcgcagaata ctccggcgac gaatgcccct gtagccagag
      961 gtaaccccag aatccaggta agcacgatgc caggaaaaac cgcatgactc atcgcgtcac
     1021 ccatcagtgc ccaacctttc aggaccagaa agaccgacag cagcgcacag ggtatggaaa
     1081 ctaccagtga aatcagcagt gcgtgactca taaaggtgaa ctgcagtggc tccagtaata
     1141 atgccatcat gagcgatcct ccagcgcttt atgcgcacgc cgacgatttg ccaaaagtcc
     1201 atgggtgggc gcaaatacaa atgctaaaag aaacagcagt gtctgcgcca ccacgatgat
     1261 tccacctgtc gcgccgtcca gaaagtaact gacccacgcg ccgaggaaac tggttacact
     1321 gccaatagtt acagcaatta tgagcaggcg gggaaaacga tcggttaaca accatgctgt
     1381 tgcaccggga gtcaccacca gacagatcac cagaaaggca ccaaccgttt gcagcgccgc
     1441 aacggtcgaa accgacagca gggtgaagaa gaggattttt aatctgcccg gatgtaatcc
     1501 aatagcacgg gcgtgattct cgtcgaaaaa ggtcaccata agatctttcc atttgaaaaa
     1561 aagcacgatg atcgagagaa tgccaatgat agtgagttgc aggatatccg ccggatcgat
     1621 tgccagaata ttgcccagga cgatggtctg aatgttcacg gatgtcgggt tcaatgacac
     1681 cataaacagc cccaggccaa aaaaggacga gaagatcagg ccgatgatgg catcttcttt
     1741 taagcgggtg cgttggttaa gaaacagcat gctgcccgct gccagtccgc cggaaaagaa
     1801 ggctcccaga gaaaacggaa gccccagcat atatgccccc gcaacacccg ggacaataga
     1861 gtgcgacagc gcatcgccaa tcagcgacca gccttttagc atcagatagc atgacaggaa
     1921 agcgcagaga ccgccgacca ttgccgagac ccacatcgca ttgagcatat attcatagct
     1981 gaagggttcc agtagcacgt tcatcttgct tccctctgca ccgctgacgg tcgatgtgcc
     2041 acaaaagggc gttcatcgtc agtaataatg ctttcttccg agccgttaag tgtgacatgg
     2101 cgcagtacgc cgctaaaagc cagctccagg ttctcggctg taaaagtggt gtctgtcggc
     2161 ccgcttgcca ggacggtgcc tttgaccatg accgtataat cgcaaaatgt cgtgactgag
     2221 ccgagattgt gcgttgagac aagcatcgtt ttgccttccg cgcgtaactc cctcaacagg
     2281 ctgatgattt tcgcttcggt tttcacatca acgccggtaa aaggttcatc cagcaggatc
     2341 acatcaccct gctgtgcaat tgctctcgcc agaaagacgc gtttcttttg tccaccggaa
     2401 agctcgccga tttgacgatg gcgaaaatcc accatatcga cacgttccag cgcatcggtg
     2461 acaatctgac gatccctctt tttggctata cgcaacatac ccatatgccc atagcggccc
     2521 atcatcacca catcttccac cagtacagga aatgaccagt caacctcttc tgactgaggg
     2581 acgtaggcaa ccaggttttt ctgtagcgcc tgtcgcgtgg gaatacccag aacagatatc
     2641 tttccgctgg tcagacgcac aaatcccata atcgctttaa acaacgtcga ttttccggaa
     2701 ccgtttaccc caaccagagc ggcaatagat ccacttggta ctgtgaagga tgcatcgcgc
     2761 agtgctgtgt gcccgttacg ccaggtgaca gtaacatcat taacgacaat gcctgcagat
     2821 tgcatcatta tttctccctt ttcccggctt taattccctg taccagcgta ctggtagtaa
     2881 ccttaagaag gtcgatatac gtaggtaccg ggccgttttc tgtgctcagg gaatcgacat
     2941 agagcacacc gccgtagtgt gcgccggttt cacgcgcaac ctgacgcgct ggtttatcgg
     3001 aaatcgtact ctcgctaaag actgccggga tatgattttt cttaactata tcaacaacct
     3061 tacgtacctg ctgcggtgtt ccttgttgat cggcattaat cggccacaga taaagctctt
     3121 ttagccccaa atcgcgtgcg agataagaaa aagccccttc actggtgacc atccatcgct
     3181 gattctcagg gagttccgta atctgcttac gcaggggggc aagggtttgg gtaatcttgg
     3241 ctttataagt atcggcatta cgttggtagg tttgtgcatt tgccggatcg tattttatca
     3301 acgcatcacg aatattatcg acgtaaatca gagcattatc tggcgacatc catgcatggg
     3361 gattaggttt gccctcatag ggtccttcgg tgatccctac tggcgtcaca cccgaagaga
     3421 caattacttc tggaacccca ttgagatgct ggtaaaagcg ttggaaccac aattccagat
     3481 tcataccatt ggcgagaatc agttgtgccc cctgcgcacg tttaatatcg ccaggggtag
     3541 gctgatactc atgaatttct gcaccaggct tggttatgga tgagacttct gcagcatctc
     3601 cagccacgtt tttggccata tccgcgatga tggtaaatgt tgtaatgacc ttgaattttt
     3661 cagttgcact tgcctgaaat gcgatcgagc aggtgagggc gaggcacccc aagagcatgg
     3721 ttactttttt tattgagtgc ataatttagt acctatagtg agcaaaatta gaacaaacat
     3781 agcaacggct atgttttata gcataaccta tacaaaatga aaataattat cattaacagc
     3841 tttgaggcct aaaacaggga ttaggttgtt tcctgctggc gacatgatat tgaggaaaaa
     3901 agtagagcag ctattaggct ttgaaatgac tttatgagga aaattgttga tggcaaaaat
     3961 ggggcaaaaa agagtgggtt atcgtagctt attgttgtcg ctgataatgt ttaacatatt
     4021 gaaaaataaa taaaatactt atgagtcaga tagttgtgat ttttgccctt acttgttcag
     4081 gttgtattgt tctttctttg taatttgttg attttcttgc attatttcag ttctctggta
     4141 ctaaatgggg caaattgggg gcaaactttg caactacgat aaccgcgcat tcaacatggc
     4201 tatctgttcg tcgttcatgt catcaatcca cataccgtaa atttcataca ccatttgcgc
     4261 agtttcatgc cccatctggc tggctataaa tgccgggttc gctcctgccg tcaacagcca
     4321 gcaggcaaaa gtatgtcgcg tatggtacgg attacggcgg cgaataccag cacgttttac
     4381 tgctgcattc catcttgtac ccaaactgct taccgagtaa taaggtttct gtttttcgtt
     4441 acacaccctg ggcatgaaaa caaaatgcag tttttgtttt tcagttctgc cgtactcccg
     4501 atgataaaaa gtgatttcgc ttttgcgatg atgcccggtc agtttgtatt gctccttcag
     4561 tgcttcaaga gccggctgca gtaatgctac cgtccggatc ccggcatttg tttttggggg
     4621 accgaacata tcaagtatcg tcaggtttct tctgacattc acaattccct tctcgaaatc
     4681 cacatcctcc cacgccagag ctgccagttc cccgtgacga agcccggagt aaacggcaaa
     4741 tttccacaag ttttggctct gtcctttttc actttccatt aatgcattga attctgtttt
     4801 agataacgg
//
LOCUS       DAJTSA010000187         4701 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000187 DAJTSA010000000
VERSION     DAJTSA010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.188"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            192..1325
                     /gene="fepE"
                     /locus_tag="OO369_004010"
                     /pseudo
     CDS             192..1325
                     /gene="fepE"
                     /locus_tag="OO369_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
     gene            complement(1322..2137)
                     /gene="fepC"
                     /locus_tag="OO369_004011"
     CDS             complement(1322..2137)
                     /gene="fepC"
                     /locus_tag="OO369_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5984075.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(2134..3126)
                     /gene="fepG"
                     /locus_tag="OO369_004012"
     CDS             complement(2134..3126)
                     /gene="fepG"
                     /locus_tag="OO369_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR5984076.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            complement(3123..4127)
                     /gene="fepD"
                     /locus_tag="OO369_004013"
     CDS             complement(3123..4127)
                     /gene="fepD"
                     /locus_tag="OO369_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR5984077.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            4238..>4701
                     /locus_tag="OO369_004014"
     CDS             4238..>4701
                     /locus_tag="OO369_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="protein p43; inner membrane protein that exports
                     enterobactin to the periplasmic space; member of the major
                     facilitator superfamily (MFS) of transporters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5984078.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTV"
BASE COUNT         1181 a         1251 c         1230 g         1039 t
ORIGIN      
        1 attacagaaa ataattatgg gttttttatt tgtttgattt ataggtttga tgaatatttc
       61 tcttaaatag agtgaatgtt gcattcagtg gaaggaattt acaaacttca gcttcggata
      121 attctcacca actggtaaag tgagcgccgt ggagattttc ccctattgga ctcattttca
      181 ggttataccc catgtcatca ttgaatatta aacagggaag tgacgctcat ttttctgagt
      241 atcctctggc gtcgcccagt aataatgaaa ttgatttact taatctaatc gaagttttat
      301 ggcgggccaa aaaaacggtc atggcggtcg tttttgcatt tgcctgcgca ggtttactga
      361 tctctttcat cctgccgcaa aaatggacca gctcagccgt tattactccc gccgaagcca
      421 ttcagtggca ggacctggag aaaaccttta ccaaactccg cgttcttgat ctcgatgtta
      481 atatcgaccg tggcggtgca tttaacctgt ttatcaagaa gtttcagtct gttagcttgc
      541 tggaagagta cctgcgttca tcaccttatg tgatggacta attaaaagag gcgaaaatcg
      601 atgaactgga tttgcatcgc gctattgttg cattgagcga aaaaatgaaa gcggttgatg
      661 acaatgctag taagaaaaaa gatgaaccgt cactgtatac atcctggacg ctaagtttta
      721 ccgcgccaac cagtaaagag gcgcagaccg ttttgagcgg gtatatcgat tatatctctg
      781 cgttggtggt gaaagagtcg atagaaaacg tccgtaataa actggagatc aaaacccagt
      841 ttgaaaaaga aaaactggct caggatcgca ttaaaacgaa aaatcaactt gatgcaaaca
      901 ttcagcgcct caattattca ctcgacattg ccaacgcggc aggaattaaa aagcccgtat
      961 acagtaatgg tcaggccgtt aaagatgacc ccgatttctc tatttccctc ggcgcagacg
     1021 gtattgaacg caaactggaa attgaaaaag cggttactga cgttgcggaa ctgaacggtg
     1081 aattacgtaa tcggcagtat cttgtcgagc aattaacaaa agcaaatgtc aacgatgtga
     1141 attttacgcc gtttaaatat cagttaagcc cgtcattgcc agtgaaaaaa gacggtccgg
     1201 gtaaggcgat tattgtgatc ctttccgcgc tgatcggcgg gatggtggct tgtggtagcg
     1261 tgttattgcg ctatgcgatg gcatccagaa aacaggatgc catgatggca gaccacttag
     1321 tttaactatt tgcggttgaa ggtgccgttc gtccgagcgg caccacaagc ggcattccgg
     1381 ctaccggatc gtcaatgatc atgcatcgca gaccataaat gcgctcaatc agttcagcag
     1441 tgacaatctc cttcggcgct ccctgagcaa caattttccc ttcccgcaat gcaatcaaat
     1501 ggctggcgta acgacaggcc tgattaagat cgtgcagcac cgccgccagg gtatagcctt
     1561 tctcgcggtt cagttcgctt aacagttcca gtagatcaat ctgatgactg atatccagcc
     1621 aggtcgtggg ttcgtcgagc agcataattg ccgtttcctg ggccagcacc atcgcgatcc
     1681 acgctcgctg gcgttgtccg ccagaaaggg tatccacgct ttgatctgcc agatgagtta
     1741 ttcccgtggc ctgcatcgct ttcgttaccg cttcttcatc ctctttgcgc cagcgggtaa
     1801 acagcggttg atgcggataa cgtccacgcg ccaccagctc ctgcacggtg atatcgcccg
     1861 gcgtggtagc attttgcgcc aacagaccaa tccggcgtgc aacttcttta ctggcgtaat
     1921 gttgaatgtg ctcgccatcc agccagacat gcccgtgagc aggcgtcatc aggcggctta
     1981 aggtacgcag taacgtggat ttaccacagc catttggtcc gataattgcc gtgaagtgac
     2041 catcaggaat ttctacagtc agattttccg caacggtata tttgccatat cccagggtta
     2101 actgttcgcc gcgcaaacgg gctactgatt cggtcatttt ttgcgagact cctgaattaa
     2161 caagacgata aggtaaatac cgccgaggct gacggtaacg acgccaaccg gaagttgata
     2221 cggcataaac agttgttggg cgcagagaac ggccgccagc agtaacagcg ctccgcacag
     2281 cgccgcctgg gtcagccccc agcgagcggt gccgctaatg cgtcgggcaa tgtgcggcgc
     2341 gactaaagca ataaaggaaa tcggcccggc aagcgccgtt gccgcagcgg taagcaccac
     2401 tgcaaccagc atcattaaca gacgcgaacg ctcgacgctg acgcccagcg cgcaggcggt
     2461 atcatcgccc atctccagca agcgcattcg ccgcaccagc aaggtggcgg caatgagcat
     2521 caatataatg atgggtgcgg aaggcgaggt ttttgcccac gtcaggccgt tgagcgatcc
     2581 ggcattccac aaacctgctg ttagcgccgt ttctaaagac gctttcagca acagccaggt
     2641 attaaaggcc accagcatgg cgcgaacgcc gataccgata ataatcaacc gaaaggtgtc
     2701 gataccgtta cgccaggcga gcagccagac caacagcgaa gtgataatgc cgcccaccat
     2761 tgctgccagc gcgatagccg tcagatcctg accaaacagc accatcgcca ccagcacgcc
     2821 gctccacgcc ccggtgttaa agcccattac gtcagggctg ccgagcgggt tacgcatcag
     2881 cgactgaaaa atcgcgccgc tgacgcccag cgccgcgcca atcaacagcg ccatcagcac
     2941 gcgtggtaaa cgccattcgg tgaccaccat cgtcatactg cgcggcgcat cgcccatcaa
     3001 cgcggcgaat acctgcgaga tttccagcgt gacggcaccg ctgcgtaatc cccagatacc
     3061 tgcaaccaca caggcggaaa tcagcagcaa acaggtgatg agtaatcggc gagagacgta
     3121 aatcatgcac cacctcgcgt tttacgtcgc acgaggaaga tcagcaccgg tgcaccaata
     3181 aacgcactga ccacagaaac gcgcagttcg ccgggcacaa tcacccgccc gatgatatcg
     3241 gcaaacagca gcagggcagg ggtagcaagt agcgtgacgg gcagcgacca gcgatgatcg
     3301 gcacctacca gccagcgcgc catgtgcggc atcatcaggc cgataaaggc aatcgggcca
     3361 actaccgccg tcgcactacc acaaagcacg gtaatcgcca gcagaccaat caactgtgtg
     3421 cgcgccacgc gactgcccag cgccgtcgcg gtgtcactgc cgaggctcaa actgttcagc
     3481 gcgcgactca gtaatagcgc agttgctccg gcgatcagaa ccgggatcag caccactttt
     3541 aaggtatgta gattgcgaat atccagcgaa ccggcttgcc agaaacgcag ttgatcgtag
     3601 acgtcaggat taagcagggc gatgccgctg gtcagtcctt ccagcaccgc cgccagcgcc
     3661 acgcccgcca gggttaaacg caccggactt aactgccctc caccctgact gccggtaaag
     3721 gcaacaatca atgaggccac cagcgccccg gcgaaggcca tcgccagttg ttcctgtgcg
     3781 gatgagtaac caaacagcgc cgcacccagc acaatggcaa agctggctcc ggcgttcacg
     3841 ccaagcaagc cggggtcggc aagtgggttt cgggtgaggg tttgcattaa cgccccggca
     3901 aggccaagcg cgccgcctgc cagtaaaccg gcaagggtac gcggcagccg cgcgtcgagc
     3961 acgatggtgc agtcggcgct ctggcaggtg ccggagaagg cctccagcac tacggaagcg
     4021 gggagtgatt ttgccccaat gagcaggctt aatgccgtcg cgataatcag taataacagc
     4081 aatccgggca cggcaatggc gcgtgtcacg gcaacagaac cagatatacg aacttccatg
     4141 ataatgaaat taattatcgt tatcgatctt atttggatat gttaacatgt gcagcctaag
     4201 aataggtatt taaaatattt gatggcaagg cattgtaatg aataaacaat cctggctgct
     4261 taacctcagc ctgttgaaaa cgcacccggc gtttcgcgca gtattcctcg ctcgttttat
     4321 ctcaattgtg tctctgggtt tgctcggcgt cgcggtgccg gtgcagatcc agatgatgac
     4381 ccattctacc tggcaggtgg ggctttcggt gacgctgacc ggcggcgcga tgtttgttgg
     4441 cctgatggtt ggcggtgtgc tggcggatcg ctatgaacgc aaaaaagtga ttttgctggc
     4501 gcgcggcacc tgtggcattg gcttcattgg actgtgcctt aatgcactgc tgccggagcc
     4561 gtcattgctg gcaatctatt tacttggttt atgggatggt tttttcgcat cacttggtgt
     4621 tacggcgcta ctggcggcaa catctgcact ggtagggcgt gaaaacttaa tgcaggccgg
     4681 ggcgatcacc atgttgaccg t
//
LOCUS       DAJTSA010000188         4649 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000188 DAJTSA010000000
VERSION     DAJTSA010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.189"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            99..283
                     /locus_tag="OO369_004015"
                     /pseudo
     CDS             99..283
                     /locus_tag="OO369_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            426..647
                     /locus_tag="OO369_004016"
                     /pseudo
     CDS             426..647
                     /locus_tag="OO369_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(668..730)
                     /locus_tag="OO369_004017"
                     /pseudo
     CDS             complement(668..730)
                     /locus_tag="OO369_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1180..2592
                     /gene="ospC1"
                     /locus_tag="OO369_004018"
     CDS             1180..2592
                     /gene="ospC1"
                     /locus_tag="OO369_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR5984079.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKSFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2696..2845)
                     /locus_tag="OO369_004019"
                     /pseudo
     CDS             complement(2696..2845)
                     /locus_tag="OO369_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2921..4618
                     /gene="ospD3"
                     /locus_tag="OO369_004020"
     CDS             2921..4618
                     /gene="ospD3"
                     /locus_tag="OO369_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR5984080.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1628 a          833 c          862 g         1326 t
ORIGIN      
        1 cgggccatat aaccacctga aagacaatga cattgcctgt ttttataacg gtaattgcag
       61 accatgacaa accgcagccg tcaggctgcc tactcgagtc acaaggctct ttgtgtccga
      121 tatgccggca gcgcattacc catgaaactg gctggaacgt acaccataag gtgaagaaag
      181 ttatgggggc ggtgaagaat tatctaacct tgtcttgtta catccaaatt gccatcgaca
      241 gttacacagt tgtgaagccg gctctctcaa aaagggctta taaaggcttg agcggtgtac
      301 cgggaaaccg gtatgcaccg ttcttagggg agggcagtcc agcaatgaac tgtccttacc
      361 cgacaaacat ccaaaacgaa cggaacgtgc tggaatccgc atataacccg ttgtaaagtg
      421 aggaaatgga cacctcactt gctcatgaga acgcccgcct gcgggcactg ttgcagacgc
      481 aacaggacac tatccaccag atggctgaat acaaccgcct gctctcacag cggatggcgg
      541 cttatgcttc cgaaatcaac cgactgaagg cgctggttgc gaaactgcag cgtatgcagt
      601 tcggtaaaag ctcagaaaaa cttcgcgcaa aaaccgaacg gcagattcca ttttcacggg
      661 cgatctacgc cacacaggct ctgggttgca gtgactgctc cattaaggcg attttgaact
      721 cataagggaa gtaacaaaaa aactgcaaaa gtacaataat tatatatggc caaactaacc
      781 tctattaaaa aacatcagtt tttcacactt agttgagctt catgaaaaaa tcatcgagga
      841 tttcattgca gaatacacat tccactaact ggaagcactc cataagagga atcagagaaa
      901 gtatcgtttt tttatagtaa aattcattgc tgttcaataa aattgatata gttattaaat
      961 tatgaaaata atttcacaaa aaagattaac ttgagttctt aagcatcgga ttttagatat
     1021 tttgaaatat ctggcatcac ataacatcaa aagtatataa tactcttatc taattacatg
     1081 cagtatattg tatatatact caaaaattgt aattcatacc ctgctatatc ttgtagggaa
     1141 agatattaat taaaactgtt ttcatataag gttcatttta tgaatatatc agaaacactg
     1201 aactcagcaa atacccaatg caatatagat tctatggata acagattaca tacattgttt
     1261 ccaaaagtga catcagtgcg aaacgctgca caacaaacta tgccagatga aaaaaattta
     1321 aaagatagtg caaatattat taaaagtttc tttaggaaaa ctatagcagc acagagttat
     1381 agtagaatgt tctctcaagg ctctaacttt aaatctttaa atatagcaat tgatgcacca
     1441 tcagacgcta aagcctcatt taaggctatt gagcaccttg acagattatc gaagcattat
     1501 atatctgaaa taagggaaaa acttcatcct ctttctgcag aggaactcaa tttgctttcg
     1561 ctaattatta attctgattt aatcttcaga catcaaagta attctgattt gtctgataaa
     1621 attttaaaca ttaagtcatt caataaaatt cagtctgaag gaatatgcac aaaacgaaac
     1681 acatacgctg atgatataaa aaaaatagct aatcatgact ttgtgttttt tggcgttgaa
     1741 atctctaacc atcagaaaaa acaccccctg aatacaaaac atcacactgt tgattttggt
     1801 gcaaatgcgt atatcattga tcatgactct ccatatggat atatgacatt aaccgatcac
     1861 tttgataatg ctattccacc tgttttttac catgagcacc aatcattttt agataaattt
     1921 tcagaggtta ataaagaagt tagtcgatac gtacatggaa gtaaaggaat tatagatgta
     1981 ccaatattca atactaaaga tatgaagtta gggctcggat tatacctgat tgactttatt
     2041 agaaaaagtg aagaccaaag cttcaaggag ttttgctatg gaaaaaatct tgcccctgtg
     2101 gatctggata gaatcataaa ctttgttttt cagccagagt accatatacc taggatggta
     2161 agtacagaaa acttcaaaaa agttaagatt agagaaatat ccttagagga ggctgttaca
     2221 gcatctaatt acgaagaaat taacaagcag gtcactaaca aaaaaattgc tctccaggct
     2281 ctttttcttt cgattactaa tcaaaaagag gatgtcgcct tatatatatt atctaatttt
     2341 gagataacta gacaagatgt tatttccata aagcatgagt tgtatgatat tgagtatcta
     2401 cttagcgctc ataattcaag ctgtaaagta cttgagtatt ttatcaataa gggattggtt
     2461 gatgtaaaca caaagttcaa aaaaactaat agtggggatt gtatgttgga taacgcaata
     2521 aaatatgaga atgcagaaat gataaaacta ttattgaaat atggtgcaac atctgacaat
     2581 aaatatattt aatcaaaatt gaatatcgtt tagcaaaagg cagtatttgc acaaaatatc
     2641 gatatattgt ttattgtcag tatggctcaa tgtgataata gttggaaagt ttgatgggtt
     2701 tcgccccgtt gtagcggtag tcgaactgga aggtcttcag gcactgctta cagcgataga
     2761 gcagcccccc tgaactggaa tggccgttcc ggataccccc ctgagtttca gagtaacggt
     2821 gacaaaccac atcaatcttt gccatcaatc atccaaaggg caaagagtac aacaacacta
     2881 agtctgcgtc acaacccatc aatgaaagga atatatacat atgccatcag taaatttaat
     2941 cccatcaagg aaaatatgtt tgcaaaatat gataaataaa gacaacgtct ctgttgagac
     3001 aatccagtct ctattgcact caaaacaatt gccatatttt tctgacaaga ggagtttttt
     3061 attaaatcta aattgccaag ttaccgatca ctctggaaga cttattgtct gtcgacattt
     3121 agcttcctac tggatagcac agtttaacaa aagtagtggt cacgtggatt atcatcactt
     3181 tgcttttccg gatgaaatta aaaattatgt ttcagtgagt gaagaagaaa aggctattaa
     3241 tgtgcctgct attatttatt ttgttgaaaa cggttcatgg ggagatatta ttttttatat
     3301 tttcaatgaa atgatttttc attccgaaaa aagcagagca ctagaaataa gtacatcaaa
     3361 tcacaatatg gcattaggct tgaagattaa agaaactaaa aatggggggg attttgtcat
     3421 tcagctttat gatcccaacc atacagcaac tcatttacga gcagagttta acaaatttaa
     3481 cttagctaaa ataaaaaaac tgactgtaga taattttctt gatgaaaaac atcagaaatg
     3541 ttatggtctt atatccgacg gtatgtctat atttgtggac agacatactc caacaagcat
     3601 gtcctccata atcagatggc ctgataattt acttcacccc aaagttattt atcacgcgat
     3661 gcgtatggga ttgactgagc taatccaaaa agtaacaaga gtcgtacaac tatctgacct
     3721 ttcagacaat acgttagaat tacttttggc agccaaaaat gacgatggtt tgtcaggatt
     3781 gcttttagct ttacaaaatg ggcattcaga tacaatctta gcatacggag aactcttgga
     3841 aacttctgga cttaaccttg ataaaacggt agaactacta actgcggaag gaatgggagg
     3901 acgaatatcg ggtttatccc aagcacttca aaatgggcat gcagaaacta tcaaaacata
     3961 cggaaggctt ctcaagaaga gagcaataaa tatcgaatac aataagctga aaaatttgct
     4021 gaccgcttat tattatgatg aagtacacag acagataccc ggactaatgt ttgctcttca
     4081 aaatggacat gcagatgcta tacgcgcata cggtgagctc attcttagcc cccctctcct
     4141 caactcagag gatattgtaa atttgctggc ctcaaggaga tatgacaatg ttcccggact
     4201 tctgttagca ttgaataatg gacaggctga tgcaatctta gcttatggtg atatcttgaa
     4261 tgaggcaaaa cttaacttgg ataaaaaagc agagctgtta gaagcgaaag attctaatgg
     4321 tttatctgga ttgtttgtag ccttgcataa tggatgtgta gaaacaatta ttgcttatgg
     4381 gaaaatactt cacactgcag accttactcc acatcaggca tcaaaattac tggcagcaga
     4441 aggcccaaat ggggtatctg gattaattat agcttttcaa aataggaatt ttgaggcaat
     4501 aaaaacttat atggaaataa taaaaaatga aaatattaca cctgaagaaa tagcagaaca
     4561 cttggacaaa aaaaatggaa gtgattttct agaaattatg aagaatataa aaagctgaat
     4621 attatctcgt ggtgatacac tgaatttga
//
LOCUS       DAJTSA010000189         4636 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000189 DAJTSA010000000
VERSION     DAJTSA010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.190"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..311
                     /locus_tag="OO369_004021"
     CDS             <1..311
                     /locus_tag="OO369_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5984081.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="OO369_004022"
     CDS             468..1217
                     /locus_tag="OO369_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR5984082.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="OO369_004023"
                     /gene_synonym="chrR"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="OO369_004023"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR5984083.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="OO369_004024"
     CDS             complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="OO369_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR5984084.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..4017
                     /gene="yieH"
                     /locus_tag="OO369_004025"
     CDS             3361..4017
                     /gene="yieH"
                     /locus_tag="OO369_004025"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR5984085.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSAIEGAGALLSAITAPMC
                     VVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
                     LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
                     "
     gene            4084..4557
                     /locus_tag="OO369_004026"
     CDS             4084..4557
                     /locus_tag="OO369_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HCR5984086.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1239 a         1162 c         1137 g         1098 t
ORIGIN      
        1 cactggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agtctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcaccagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aacgtttcaa aggtgtaaaa ctgtacgaaa ttatcgatat tgtttccctt
     3541 gaacatggtg ttacgttagc gaaaacagaa gctgaacacg tttaccgtgc agaagtcgct
     3601 cggctgttcg attcagccat cgaaggggct ggagcgctcc tgtcagcgat cactgcgcca
     3661 atgtgtgtgg tatctaacgg cccaaataac aaaatgcagc attctatggg caagctgaat
     3721 atgttgcact acttcccgga taaactgttc agcggctacg atattcagcg ctggaagcca
     3781 gacccggcgt taatgttcca tgcggcaaaa gcgatgaatg taaatgtaga aaactgcatt
     3841 ctggttgatg actcagttgc cggtgcgcaa tctggtatcg acgcaggtat ggaagtgttc
     3901 tacttctgcg ccgacccgca caataagccg atcgttcacc cgaaagtcac cacctttacc
     3961 catctttcgc agttaccgga actgtggaaa gcgcgtggtt gggatattac ggcatagttc
     4021 ttcacactcc cttcacttac cccgcttaaa ttggcgctca aaggtaagta aagggagttt
     4081 gatatgtctg tttcacgtcg ggtaatacat cacggacttt attttgcggt tttaggaccg
     4141 ttaattggtg ttctgtttct tgtcctctac atattcttcg caaaagaacc gctggttctt
     4201 ttggtaataa tacaggtact tccattgttt ctcttattgt cgattactac gggagctatt
     4261 cctgcattgt taaccggcgt gatggtcgcc tgtctgccgg aaaagatcgg gtcacagaaa
     4321 aattatcgtt gtctggctgg tggcataggt ggcgtcgtta tcaccgagat ctattgtgca
     4381 gttattgtac atattaaggg catggcttcc tcggagttgt ttgaaaacat tctttctggt
     4441 gacagtctcg ttgtccgcat cattcctgca ttgctggcag gtgtggtgat gagcagaatc
     4501 attacccgtc tgcccggatt ggatatttca tgtcctgaaa cagactcttt aagttaagcg
     4561 ggatacttta tctttgggct actcaaaagc agacaggatg ttactatgac tcaaaatatc
     4621 aggccgttac cccaat
//
LOCUS       DAJTSA010000190         4630 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000190 DAJTSA010000000
VERSION     DAJTSA010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.191"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..369
                     /gene="bglB"
                     /locus_tag="OO369_004027"
     CDS             <1..369
                     /gene="bglB"
                     /locus_tag="OO369_004027"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR5984087.1"
                     /translation="RVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLN
                     DHLVQVNEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTR
                     KKSFGWYAEVIKTRGLSLKK"
     gene            455..2071
                     /gene="bglH"
                     /locus_tag="OO369_004028"
     CDS             455..2071
                     /gene="bglH"
                     /locus_tag="OO369_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCR5984088.1"
                     /translation="MFRQNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMIDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNNIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDAQFAVGAQAEIWW"
     gene            2098..3273
                     /locus_tag="OO369_004029"
     CDS             2098..3273
                     /locus_tag="OO369_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCR5984089.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFSVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKFKIRH"
     gene            3275..3997
                     /locus_tag="OO369_004030"
     CDS             3275..3997
                     /locus_tag="OO369_004030"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5984090.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(4059..>4630)
                     /locus_tag="OO369_004031"
     CDS             complement(4059..>4630)
                     /locus_tag="OO369_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCR5984091.1"
                     /translation="GALTFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSGPFYCVD
                     EVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELV
                     ERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLA
                     KCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
BASE COUNT         1283 a         1026 c         1131 g         1190 t
ORIGIN      
        1 cgggttctgt taaatacgct ttgggatcgt tatcaaaaac cgttatttat tgtcgagaat
       61 ggattaggcg taaaagacag cgttgaagcg gatggttcga tacaggacga ttatcgaatt
      121 gcctatttaa acgatcacct ggtacaggta aatgaagcga ttgccgatgg tgtggatatt
      181 atggggtaca ccagttgggg gccaattgat ttagtcagtg catctcattc acaaatgtct
      241 aagcgctacg gctttattta tgtggatcgt gatgataatg gcgaaggaag cctcacaaga
      301 acgcgtaaga aaagcttcgg atggtatgca gaagtgatca aaacgcgggg gctgtcatta
      361 aaaaaataac caataaagca ccttaattat cgtcgcattc agaacagtct ggatgcgata
      421 cgttaattct ttctttgcac cataaaggga tattatgttt agacaaaatc ttattacctc
      481 tgccatctta ttaatggcac cgttagcctt ttctgcacaa tcattggctg aatcattaac
      541 ggtggaacaa cgccttgagt tattagaaaa agcgttaaga gaaacgcaaa gcgaactcaa
      601 aaagtataaa gatgaagaga agaaaaaata tacgccagcg acggtgaatc gtagcgtaag
      661 tacgaatgat caagggtatg ccgccaatcc gttcccgacc agtagtgccg caaaacctga
      721 tgctgtactg gtcaaaaatg aagagaaaaa tgccagtgag acaggctcga tttattcttc
      781 catgactctg aaagatttca gtaagtttgt gaaagatgaa attggcttta gttacaacgg
      841 ctattaccgt tctggttggg ggaccgcctc tcatggttca cctaaatcat gggcgattgg
      901 ttctctgggc cgctttggta acgaatactc cggctggttt gatttgcagt taaaacaacg
      961 tgtctacaac gaaaacggca aacgggttga tgccgttgtg atgatagatg gtaacgttgg
     1021 tcagcagtac tctaccggct ggtttggcga taatgccggt ggcgagaact ttatgcagtt
     1081 ctccgatatg tacgttacca ccaaaggttt cctgcccttt gcgccagagg ctgatttctg
     1141 ggtgggtaaa cacggtgcgc cgaaaattga aatccagatg ctggactgga aaacgcagcg
     1201 tactgatgct gcagcgggtg taggtctgga aaactggaaa gtcggtccgg gtaaaattga
     1261 tatcgcgctg gttcgcgaag atgtcgatga ttacgatcgc agcctgcaaa acaaacagca
     1321 gattaatacc catactattg atttacgcta taaagatatc ccgttatggg ataaagcgac
     1381 cttaatggtg agtggtcgtt atgtcacggc aaacgaaagc gcatcggaaa aagataatca
     1441 ggataataac gggtattatg actggaaaga tacctggatg ttcggcacat ctttaacgca
     1501 gaaatttgat aaaggtggct tcaacgaatt ctccttcctg gttgcgaata actctatcgc
     1561 caggaacttt ggccgttatg ctggcgcaag tccatttacc acctttaatg gtcgttatta
     1621 tggtgatcac accggcggaa cagcagttcg tctgacctcg cagggcgaag cctatatcgg
     1681 cgatcatttt attgtggcta acgcgattgt ttactccttc ggtaacaata tttatagcta
     1741 cgaaacaggc gcacactctg atttcgaatc tattcgtgcg gttgttcgcc cggcctatat
     1801 ttgggaccaa tataaccaga caggtgttga actgggctat ttcacccagc aaaacaaaga
     1861 tgcgaatagt aataaattta atgagtctgg ttataaaacc acgctcttcc atacctttaa
     1921 agtcaatacc agtatgttga cctcgcgtct ggaaattcgt ttctacgcca cgtatatcaa
     1981 agccctggaa aacgaactgg atggcttcac cttcgaagac aataaagacg cccagtttgc
     2041 tgtcggtgcc caggctgaaa tctggtggta atggaactta atgaaaggaa tattacgatg
     2101 aatataaaaa ttgctgcttt aacgctggct attgccagcg gtatttctgc tcagtgggcc
     2161 atagcagcgg atatgccagc cagcccggca cccactatcc cggtgaaaca gtatgtgact
     2221 caggtcaatg ccgataacag cgtgaccttt cgctactttg cccctggggc aaaaaatgtc
     2281 tctgtagtgg tgggtgttcc ggttccggac aatattcacc cgatgaccaa agacgaagca
     2341 ggagtctggt cgtggcgcac acctatcctg aaaggcaacc tgtacgagta ttttttcaat
     2401 gttgatggtg tacgcagcat tgatacaggc accgcaatga ctaagcctca gcgccaggtt
     2461 aactccagta tgattctggt gccaggcagt tatctggata cgcgttctgt tgcgcatggt
     2521 gatttgatcg ccataactta ccactccaac gcattgcaat ctgaacgtca gatgtatgtc
     2581 tggaccccgc caggatacac cggcatgggc gagcctttgc cagtgctcta tttctatcac
     2641 ggttttggtg ataccggacg ttccgctatc gatcaggggc gtatcccgca aatcatggat
     2701 aacctgctgg ctgaagggaa aattaaaccg atgctggtgg tgatcccgga taccgaaacc
     2761 gatgcgaagg gcattattcc cgaagatttc gtgcctcagg aaagacgtaa agtcttttat
     2821 ccgctgaatg ctaaagcggc agatcgcgaa ctgatgaacg atattatccc gctgattagc
     2881 aagcgtttta gtgtccgtaa agatgccgat ggtcgcgcgc tggcagggct ttcacaaggc
     2941 gggtaccagg cgctggtttc cggaatgaat catctggaaa gctttggctg gctggccaca
     3001 ttcagtggtg ttaccacgac aaccgtaccg gatgaaggtg tcgcggcccg gctgaacgat
     3061 ccggcagcta ttaaccagca actacgtaat tttactgtgg tcgttggtga taaagatgtc
     3121 gtaaccggca aggatatcgc cgggctgaaa actgagcttg agcagaaaaa aattaagttt
     3181 gattaccagg aatatcccgg cctgaaccat gaaatggatg tctggcggcc tgcctatgca
     3241 gccttcgtac agaaatttaa aataaggcat taagatgaaa ttaatcatta ccgaagatta
     3301 ccaggaaatg agccgtgtcg cggcacacca tctgcttggt tatatgtcga agacgcgtcg
     3361 tgttaacctg gcaattaccg ccggtagcac gcccaaaggc atgtatgaat acctcaccac
     3421 cctggttaaa ggtaagcctt ggtacgataa ctgctatttc tataatttcg atgaaattcc
     3481 ctttcgcggc aaagagggag aaggcgtaac gattaccaat ctgcgtaatc tgtttttcac
     3541 ccctgcgggg atcaaagaag agaatatcca gaagctcact attgataact accgcgagca
     3601 tgatcagaaa ctggcgcgtg aaggcggcct ggatttggtg gtgctgggat taggtgcaga
     3661 tggtcatttt tgtggcaatt tgccgaatac gacccatttc catgagcaaa ccgtggagtt
     3721 cccgatccag ggagagatgg ttgatattgt ggcgcatggt gagttgggcg gcgacttttc
     3781 gttagtgcct gacagctacg tgacgatggg gccgaaaagc atcatggcgg cgaaaaactt
     3841 gctgataatt gtcagtggcg caggtaaagc gcaggcactg aaaaatgtgc tgcaaggtcc
     3901 ggttaccgaa gatgttccag cttccgtgtt gcagctgcat ccttcgctga tggtaatcgc
     3961 tgataaagcc gctgccgcag aattagcgtt cggttaagct gaagaaataa ggccggatgt
     4021 atgaattacg ctccggcctg ttctcattat ttaaataact acgaaaaatc accccacagt
     4081 ctcagcttgc cgcagtggag acagcggaag agataaccct gacaatcacc acccttttgc
     4141 aggcatttag ctagatcaga atttctaata ccgaaattct cacaatcttc ttcaaggttg
     4201 gcaaattcat cgaggcgatc ttttatatca ttccagccca catagccgat aaaagcacag
     4261 aaatcgccac aatgcgcaag ccagaattcc tgttgccatc catgataacc tggcgtgcgt
     4321 tcaaccaact ctttcagcat ttcatcagga tatgtgttct taataccggc aaattcgtcc
     4381 tcttcatcat actcaaattc aacaccttct atgctggcat catcctgaaa actacctgca
     4441 aacttttctg cagcagaacc gtccgcaata cacaacggac agagatgttc aacttcatca
     4501 acgcaataaa agggacccga gtaataaact gacgtctgtt gttcacagca atcgcactct
     4561 acggttttat cctgttcaaa tgcgcctgtt tccagtggct tgggatgata tttgaatgta
     4621 agcgccccga
//
LOCUS       DAJTSA010000191         4603 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000191 DAJTSA010000000
VERSION     DAJTSA010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.192"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(79..867)
                     /gene="phoH"
                     /locus_tag="OO369_004032"
     CDS             complement(79..867)
                     /gene="phoH"
                     /locus_tag="OO369_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR5984092.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1474..2739)
                     /gene="efeB"
                     /locus_tag="OO369_004033"
     CDS             complement(1474..2739)
                     /gene="efeB"
                     /locus_tag="OO369_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR5984093.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2745..3872)
                     /gene="efeO"
                     /locus_tag="OO369_004034"
     CDS             complement(2745..3872)
                     /gene="efeO"
                     /locus_tag="OO369_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR5984094.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3930..>4603)
                     /locus_tag="OO369_004035"
     CDS             complement(3930..>4603)
                     /locus_tag="OO369_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR5984095.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1081 a         1296 c         1109 g         1117 t
ORIGIN      
        1 tttcagaaaa agcgctagtc cgggcataaa cacccaaaac aaagcccggt tcgcccgggc
       61 tctgcaccga taatacactt aactgtatgc atgcagcgta cgttggcaaa gtgccgaacg
      121 tacgcaatcc tcttttccga accggacgat cccaaccatt tcatcttctt cgaaacgttc
      181 cagcgcgtca cttaatccgg agcacacgcc gcgaggcaaa tcgcattgcg tgatatcacc
      241 gttgacgata accgtcacgt tctccccgag gcgggttaaa aacattttca tttgcgcggc
      301 agtcacattc tgcgcctcgt caagaatgac aactgcattt tcaaaggtac gtccacgcat
      361 ataggcgaac ggcgcggttt ccaccttccc tatttccggt cgcaggcagt actgcataaa
      421 ggaagcccct aagcgccgga ccagcaagtc gtagaccggg cgaaaatagg gagcaaactt
      481 ttctgcgata tcaccaggta agaagccaag atcttcatcg gcttgcagaa ctggacgggt
      541 gacgataatc ctgtcgacat ccttatgtat cagggcctct gccgcttttg ctgcgctgat
      601 ccaggttttt ccgcacccgg cttcgcccgt ggcgaatatc agctgcttac tctcaatagc
      661 ctgcagatag tgcaattgcg cttcatttcg cgcaaggatg ggcgaggtat cgcgactgtc
      721 gcgggccata ccaatggctt ctacgccgct catctgcaca agcgaggtga ccgattcttc
      781 ttcacgctgc ttatggctgc gcgaatcccg tctcagcaca cgttttgcct cgcgacgagc
      841 tttgatcact gctttttgtc ttcccatgga gagcaccttg agttgtttgt attcatcaca
      901 cgcgccgttg gcagcgcgat tatgcgcacg aacatcagag ggttggcttc cttgtaagcc
      961 atagtttgct tttggataaa atgccgaaaa acggctacgc acaccgtttg cggcgtcggt
     1021 aacacgagta aagaaaggaa gtgagaaaaa tacagagtga cgaagttgct accggaggag
     1081 aaatctccgc gagtggtgga gcgttgctta acaaaacatt gtccagaaca ggacgttacc
     1141 atccgcgatc tccatagtga ctgactatca ctgccgggaa cttccgctgc tacttaataa
     1201 gtacaacaga actcgcattc attgcaacaa tatattcact tatatttaac gataacaccc
     1261 atttcggtga cattaatttc tactggaaag atgacagagt gatgacagtg atggaaaaag
     1321 ctgtgtgctt tccgcaggat tcgcagggct ggtaaaatgt gcctcgtgtt tgccggattc
     1381 ggcgcgaacg ccttacccga cctacaagtg attaataatt tgtgcacaaa actcatgccc
     1441 gataaacctg cgcatcgggc atatttgcgt cgttcaaacc tgcaatagcg cccttcccag
     1501 ataatcgttc gcgtccttca ctcccggcag cgaaaaaaaa taaccgccgc cgataggttt
     1561 aacgtattcc tccagcgctt cgccattgag ccttttttgt actgtcagga agcctttttc
     1621 cagatcgtgt tggtagcaga caaacagcaa ccccatatcc agttgtccgg agttggtgac
     1681 ccccaacgaa taactgtagc cgcgacgcag catcaggctg gactcactct ccgccgtgcg
     1741 gggattcgcc agccggatat ggctgtccag cgcgatcacc ttcccttccg ggtcgctggc
     1801 gtaatcaggc acatcatgct catgctgcat tcccagcggc gcaccggttt gcttatcacg
     1861 gccaaaaatc gtctgctgtt ctttcagcgg cgttctgtcc caaaattcca ctcgaaactg
     1921 aatcaagcgt actgcctgat agctgccacc gattgtccac gcaggctcct gctgatctgc
     1981 cgttacccac accacttttt gcatcaactt atcattctgg ctatcgggat tggcagtgcc
     2041 gtctttgaaa cccagcaaat taatcggcgt ctctttgcct ttactacgcg ccgcgtgatc
     2101 ggaaataaac ccttcccgct tccagcgcac actgagcaaa tccggcgtgt gtttgatgat
     2161 atcgcgcagc gcatggttca ccgtgtcctg ggtgttggcg caaatctgta gcaacacatc
     2221 accatgacat aacgccgcat ccagcgagtc gttggggaaa cgcgtcatct tctgcagctt
     2281 ttttggcatc tgtggcgcaa ggccaaagcg ctcatcaaac aatgagtgac ccaccgataa
     2341 cgtgatggtg agattatcgg gcgcaatgta gccgccaaga atgccggaat cgagtggtgg
     2401 caggcgcgga tttggcgttt ctggtgctgc tccgccctga ctcagaaaag caaaacgctg
     2461 agtcaacaag cgaaacaacc gctcaagatc ggctttatcg ctggcaagca catcaaacgc
     2521 caccagcatc attgcggcct gttgtggcgt caggatccct gcctgatgct caccataaaa
     2581 cggctgtttc tcattgcgtg catccggtga aagcgtgccc ggtgcacttt gcgttttttg
     2641 tgcatgagcg accggacaac ttcccgccag cgcaccaatc cctttcagta aacgtcggcg
     2701 tgacggttca ttcacgccgt tttcatcttt atactgcata acgcttaatc cagtcccagc
     2761 acaccgcgaa gttgcgccag atcttccgcc agcgcagtaa tcggcccttt cagcgcattc
     2821 cggtcggcat cggtcaattt gtcgtaggtt tcaaaaccgt ctttagtacg gtatttcgcc
     2881 agaatggtat cgaccttttt aaagttggca tcgacttttg ccaacagttc cgggttggct
     2941 ttttgcagtt gtggacgcag cagatcgaca attttctgcg agccttcaac gttagcctgg
     3001 aaatcccaca gatcggtgtg gctgtagcga tcttcttcac cgctgatttt gctggctgcc
     3061 acttcctcaa tcagtccggc tgcgccgccg accacttttg aaggtgggaa agccagttca
     3121 ctgatgcgtt tttgcaaatc gaccacatcg gtataaagct gctcagcgta ctgatccatc
     3181 cctttggtgg tgttgtcgcc aaacaatgct ttttccagac ggtggaaacc agtgaatttt
     3241 gggtcggcgg ctttttgctc gtaatcatct tcacgggcgt caatgctgcc atccagatcg
     3301 gagaacagtt cagcaatcgg ttcaatacgc tcatagtgct ggcgcgtcgg cgcatacagt
     3361 gctttcgctt tttcgatatc gcctgcttta atcgcgtcgg taaaggcttt ggtgtcggtc
     3421 accagctgcg tggtttccgc catgacatac gctttatatg cagtaattgc accaccaaga
     3481 cttaacagcg catcactttg cgccgcatcc gccgttgcct cacctttgac gatcaacttc
     3541 cctttcgggt tagtcagcag accgcaggtc atatcgtatt cgccaggctg taaattcgcc
     3601 gtcattttct ggctaaaacc aggggcgata ttttcccgct cttccaccac catcacgcct
     3661 ttgaggatct cccactccag cgccttctgg ctatggttct gaataatgaa ctgtgttttc
     3721 ccggcgttaa ccgtaatggt catcggttcg cactgcttat ccgtcacggt cactttgacc
     3781 tgcggcacat cagcggcgtt agccataaaa gcagaagaaa acagcgcagc cacgctcaac
     3841 tgcaatgcgt tacggcggaa gttaatggtc atgacgagac tatcccttta aagtatgttg
     3901 taactaagca agagtttgcg tcgtatttgt tacgcggagc gagacgctgt cgcccctgcg
     3961 cgtggtggca gagcaaatgc caccagcgcc gggatgagat aaataaacca gacggcgact
     4021 tcgctgacgc tcggtgcttc ctgatagcca aaaatccctt ccatcagcgt gccaaacagc
     4081 gagtgagttg agaggaccgc gctcatatcg aaggcgattt cctgaaagtg gttccacaat
     4141 ccggcttcat gaaatgcgcg aatggcacca gccgccagcc ctgcggcgac gaagagaata
     4201 aacaggctgg tccatttaaa aaatgcacca agattgaggc gaataccgcc ccagtagagc
     4261 aggaagccga gcaccacggc agtagcaaga ccgagcattg cacccagcgg cggccagatc
     4321 ccgacatctt gttgaaatgc cgccagcagg aaaaagaccg actccagccc ttcccttgca
     4381 acggcaaaaa agaccatcat caccagcgcc cagccatgat gatttccacg ctgcaatgcg
     4441 ctatcgactg cctgttccag ttgcactttg acgttgcgcg acactttgcg catccagaaa
     4501 accatccagg taaggatcac cacggcgatc accgccacga taccttcaaa cagttcctgc
     4561 tctttttgcg gaaattcgcc ggtggtttcg ttaatgaaga tcc
//
LOCUS       DAJTSA010000192         4550 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000192 DAJTSA010000000
VERSION     DAJTSA010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.193"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1224
                     /locus_tag="OO369_004036"
     CDS             <1..1224
                     /locus_tag="OO369_004036"
                     /inference="COORDINATES: protein motif:HMM:NF027197.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR5984096.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEK
                     WFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILP
                     VIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHL
                     LIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRA
                     ARVFVNDERFTRGGN"
     gene            1224..1958
                     /locus_tag="OO369_004037"
     CDS             1224..1958
                     /locus_tag="OO369_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5984097.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            2426..3187
                     /locus_tag="OO369_004038"
     CDS             2426..3187
                     /locus_tag="OO369_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5984098.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3231..4310
                     /locus_tag="OO369_004039"
     CDS             3231..4310
                     /locus_tag="OO369_004039"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5984099.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4307..>4550
                     /locus_tag="OO369_004040"
     CDS             4307..>4550
                     /locus_tag="OO369_004040"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5984100.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQ"
BASE COUNT         1247 a          864 c         1120 g         1319 t
ORIGIN      
        1 tttccgtatg gcattttcca gcagcgaaat gttcttgcga gctggctggc aacaggttgc
       61 ggcgtagcgt tgtatctggc tttaacggcg cgtactcgtt ctctgacgtt gatatgtttg
      121 ttgtcgttat accctctgtc tacagcttta gtccttacac aaagccgtgt cggccttctt
      181 ggtttatttt gcgtcatcgt gctggccgct ctcgcggaca gaccccgctg gcgcggacgt
      241 ccccttagcg gtctgttgcg cttgatcttc gtcgcctcct tgctgtgctg gtgtgtcggt
      301 atctcaaact atgccatgcc aggggagaca acggctgact ttacgcattc atcatcgaat
      361 gaccagcgta tacgtattct gaaaggtgcc ataagtctta ttcaacagcg ccccttaacc
      421 ggttgggggt taggcagctt tgaagcgacc ttcccgcaag ccttagagga tgtgggcgtg
      481 acaaacatag agagtgatac ggctacgcat ccccataacg aactgctgtt cgtaacggct
      541 gaaggtggca tcgtcgcctt gtttggtttt ttgctgctat caggcgtttg gctgtggccg
      601 tgtatatgcc gcctgaaaat gacaaacaat aacgcgcacc atcaatgtga aaagtggttt
      661 attgccttaa tggggttgcc tttagtcatt catatgatga ccgaatatcc tctctatacg
      721 tctgttcccc atttgatgct gttgctgctc cttttccgca tcggaatgcc ggaaaacgtg
      781 atgaatcagg tacgtatacc taaaacagtc cggttgggta ttctgcctgt cattgtgctc
      841 attatgaccg ccgcgctggt catcctggta gcgggatttg atgcacagcg ggaactgacc
      901 agagctgaag ctgatatgaa cgaaggattt atccctcgcc ttccggaaca tagctggaaa
      961 tacctgacgc aggcagaacg tcttgagcgc gatcagcatc tgcttattgc gaataaaccg
     1021 gggttcgttc gcgatacgga aagcatgtct cttttcactg tgtggggaaa taaatggttg
     1081 gcaatccaca atgatgcaga cgtaagcgct gcaatgatat tcattgcgca caggcgcggt
     1141 gattatctga cagctgaaaa aataagaaaa cgcgcagctc gggtatttgt aaatgacgaa
     1201 cgttttactc gcgggggaaa ctaatggtta acgatctgac gtataagaaa ttagaaagca
     1261 caaacatcaa ctgtaatatt ctctcgccag aaaataccac atggacaaat gtcacggtga
     1321 ttggcctgga tctttttgcc atttgcggcg tgtgcagttt gctcaagcgt gttgggggag
     1381 tgaaacttca ggttttcact tccttaccca ctgatatgaa tgttctgact gacagtagca
     1441 tcgtgatatg ggtaaaaatg cgtcacgatg gtttgcctga attagccgga catattgtga
     1501 aggtttgtcg tcgataccgt ctgatgcggc aactcgttat tagcgatgct attcctgaag
     1561 gaatgacgaa tgatatcggg cctctttccc gagtatggct tactcatggg agtgcaaaag
     1621 tggatatttt attatcttca ttacggaagg tgttaaatgc tcattgtttg cctggtcctt
     1681 tgtttataaa acgcctggga agagtgcaat ggagggttct tttactccgc gcgaagggga
     1741 ttagcacacg taaaattgca gaaatctgtg gtattggcct caagaccgtt agcgcccatg
     1801 agtccgcaat tcgagaacga ttaggcacca ggaataaaag tgagtacgtc tggttagtac
     1861 gtaatgctca acttatacaa caggcgatac ccgcgttggg aagagatgcg tcaaggtatg
     1921 aaagatgtaa caatatgcga acaaagcaaa aagtataatt gatgcgtcat agataactct
     1981 ctttttcgat gacgaaatcg ctgagcatgc gtggtgaggt tagtaataga tgggtgctat
     2041 gttttgccac gggtttgtag ctgtgaaacc tggaaaaaaa taagaattaa aaatatttaa
     2101 agagagttaa taaggttctg gttgtaattg tcttggtcaa aaaaaagata ttgtcaatgt
     2161 tgttttttaa attttcaggt tttggttaaa tatttgcttg caaatatagg tgattttttt
     2221 attgtaggct tttgaaggct attataactg tgcgctaaat taatgcgttg aataaaagaa
     2281 ataacaaaat taagaaaagc catatttacg atatgggcaa cataaatata atctaaaaaa
     2341 gagtaaggcg ttgtaggttg gttccgcgtt cattaccttg ttagtgaacg cggatttatc
     2401 agttgtcgaa atggttttta taataatgaa aaattttaat gtactaacca aagtcgtatt
     2461 ttgcttgtcg tttttatcaa gcgcatttcc cacctatgct gcggatgatg tcaaagtgac
     2521 tcaaaacact tcagttttta aactgaagct tagtgcaacg cgattaatct atgaacaagg
     2581 gtcggatggc gcagcgataa ctgtgacaaa tccgcaggat tggccgatgc ttgtgcagag
     2641 taaagtcttt gaagaagata aagtaactgc agcgcccttt attgtcactc cgccactgtt
     2701 caggcttaat ccaggccagc aaaataaaat gtgggttgtg cagactgcgc cagctttaca
     2761 agacagggaa aaattacact ggctatgcgc caccggcatt acgcctaaaa agagtgatgt
     2821 ttgggccgag aaagataaag aaaaaattgc tgaaaatgtg aacctgaaca tccaggttaa
     2881 cgtacatacc tgcataaaac ttattaccag gccatctggt attgaaggca agatggttaa
     2941 tgcgagtgag aagttatcgt ggaaaaagca gggtgataaa attgtcgcgg tcaatcagtc
     3001 gccgttctac atcaaccttt cttctttaca ggttggcggt agcaagattg agaaactaga
     3061 ttatattgcg ccgttttcgc aacatgagtt tgttatgcca aaaactagcg gccaggatat
     3121 taaatggtct gtgattactg atgaaggtgg tgagagtcgt ttgtttatat ctcatttgca
     3181 atcataatgt attacatttt tattgaatat ttttatgcca tgcatattcc atgttttatc
     3241 gactgtcatt aattttgata atggccttac ttgcgggtca gttaagtgcc caggagtggt
     3301 catttgattc ttcacagttg gaaggaaacg ttagtgcgga cactgtggca atgtttaatc
     3361 agggagagca actccccgga aattatcgtg tggaaattta tcttaacggt gaaaaagttg
     3421 atgttgggga gtttcccttt catcgtccgg aatcccctga agaaaaagag cttgttccat
     3481 gtttaaccgt tgatgacctt atccactatg gcattaaaat tgataaaagc agcagtgata
     3541 ctgacaataa aaagaatcag tgttttaaat ggaactctat tgaaggtttg aaagtaaatt
     3601 atgattttga tagccagcgc gtacagataa ccgttccaca actgtatctg caagataaaa
     3661 aaagtagttt agcaccagtg tcgttatgga atgagggtgt agctgcattc cgcatggtgt
     3721 atcagacaaa tattgatatt tccaaacaaa atgataatca atctaccacg cgtaattctc
     3781 gttatggcag gttcactcca gggtttaatc ttggtgcctg gaggtttcgt agttcagtga
     3841 catggagcaa ggaacttggc cagtcagaaa gatggcaaag gggttacatg tggtttgagc
     3901 gaggcataaa tgcgattaaa agccgattaa cgcttggaga atcttatacg tcgtctgagg
     3961 tgtttgacag catacctttt cgcggcggga tgttagcgac cgacgatgcg atgactccgc
     4021 cggaggacag ctactacaca cctgtggtgc atggaatagc acaaagtgag gcgcaggtta
     4081 ttattaaaca aaatgggcaa attattttta cccgttctgt gccgccaggg ccttttgcgc
     4141 tggataattt accaacgctg gccgttggcg gggagcttga tgttacagtg agagagagta
     4201 atggcgagga gcagtatttt tccgtgccat ttcaaacgcc ggccatcgct ctgcatgaag
     4261 gatatttcaa atatagtgtg atggggggga atataaagaa gaaggtataa aaagctcccc
     4321 tgtctccgtg ggcgaaatca cggcgatgta cgggcttcct tatggcgtca cggtgtatgg
     4381 agggattcaa aacgccactc attttaatgc aatatctacg ggtattggca tttctctggg
     4441 gctgttaggg agtttatcga cagatattac gcgttcgatt gcgaatttat attatgggaa
     4501 taaatatcgt attcgttata gtaaatccat tagtgatttt ggtactcaat
//
LOCUS       DAJTSA010000193         4329 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000193 DAJTSA010000000
VERSION     DAJTSA010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.194"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..258)
                     /locus_tag="OO369_004041"
     CDS             complement(<1..258)
                     /locus_tag="OO369_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984101.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMS"
     gene            283..522
                     /gene="relB"
                     /locus_tag="OO369_004042"
     CDS             283..522
                     /gene="relB"
                     /locus_tag="OO369_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCR5984102.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            522..809
                     /gene="relE"
                     /locus_tag="OO369_004043"
     CDS             522..809
                     /gene="relE"
                     /locus_tag="OO369_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCR5984103.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            881..1036
                     /gene="hokD"
                     /locus_tag="OO369_004044"
     CDS             881..1036
                     /gene="hokD"
                     /locus_tag="OO369_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5984104.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1254..1505
                     /locus_tag="OO369_004045"
     CDS             1254..1505
                     /locus_tag="OO369_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984105.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            1572..1850
                     /locus_tag="OO369_004046"
                     /pseudo
     CDS             1572..1850
                     /locus_tag="OO369_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1852..2910
                     /locus_tag="OO369_004047"
     CDS             1852..2910
                     /locus_tag="OO369_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5984106.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNPPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            2911..3276
                     /locus_tag="OO369_004048"
     CDS             2911..3276
                     /locus_tag="OO369_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5984107.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            3273..3956
                     /locus_tag="OO369_004049"
     CDS             3273..3956
                     /locus_tag="OO369_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5984108.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
     gene            4155..4230
                     /locus_tag="OO369_004050"
     tRNA            4155..4230
                     /locus_tag="OO369_004050"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4188..4190,aa:Ile,seq:cat)
     gene            4232..4308
                     /locus_tag="OO369_004051"
     tRNA            4232..4308
                     /locus_tag="OO369_004051"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4266..4268,aa:Lys,seq:ttt)
BASE COUNT         1152 a          980 c         1158 g         1039 t
ORIGIN      
        1 tgacatgcta aagcaaaaaa tagatgaata agttgagttg tgcatatgta gcctgaccgt
       61 cacaaagtat atggtgtctg taccagtaag atgatggccg gactctttaa aaaagagctg
      121 acctgcacaa tacaggatgg acttagcaat ggctgctcct ggcacaaagc ggacagtgat
      181 caccgttctt acgactactt tctgacttcc ttcgtgactt gccctaagca tgttgtagtg
      241 cgatacttgt aatgacattt gtaattacaa gaggtgtaag acatgggtag cattaacctg
      301 cgtattgacg atgaacttaa agcgcgttct tacgccgcgc ttgaaaaaat gggtgtaact
      361 ccttctgaag cgcttcgtct catgctcgag tatatcgctg acaatgaacg cttgccgttc
      421 aaacagacac tcctgagtga tgaagatgct gaacttgtgg agatagtgaa agaacggctt
      481 cgtaatccta agccagtacg tgtgacgctg gatgaactct gatggcgtat tttctggatt
      541 ttgacgagcg ggcactaaag gaatggcgaa agctgggctc gacggtacgt gaacagttga
      601 aaaagaagct ggttgaagta cttgagtcac cccggattga agcaaacaag ctccgtggta
      661 tgcctgattg ttacaagatt aagctccggt cttcaggcta tcgccttgta taccaggtta
      721 tagacgagaa agttgtcgtt ttcgtgattt ctgttgggaa aagagaacgc tcggaagtat
      781 atagcgaggc ggtcaaacgc attctctgaa ccaaagcatg acatctctgt ttcgcaccga
      841 aggtgacact tctgctttgc gttgacagga gaagcaggct atgaagcagc aaaaggcgat
      901 gttaatcgcc ctgatcgtca tctgtttaac cgtcatagtg acggcactgg taacgaggaa
      961 agacctctgc gaggtacgaa tccgaaccgg ccagacggag gtcgctgtct tcacagctta
     1021 cgaacctgag gagtaagaga cccggcgagt gagaaatccc tcgccacctc tgatgtggca
     1081 ggcatcctca acgcacccgc acttaacccg cttcggcggg tttttgtttt tattttcaac
     1141 gcatttgaag ttctggatgg tgccggaata gaatcaaaaa tacttaagta gcgcgcaggg
     1201 ataaaaggga tggaccccga acaggggagt gctacttatc tggaaggatt ctgatgatga
     1261 acatcgaaga actgcgtaaa attttttgtg aagatggcct ctatgctgtg tgcgttgaaa
     1321 atggaaatat tgttagtcat taccgcattg tgtgtttgca aaaaaatggg gctgcgttaa
     1381 ttaattttgt ggatgcccga gtgacggacg gatttatctt gcgcgacggt gagtttgtca
     1441 cttcattaca ggcattgaaa gagatcggaa taaaagctgg cttttctgct ttttcagaag
     1501 aataaactca tctacaatct tgcgcggggc tgaactcccg ctgagtaaca ccgtgccacc
     1561 ggagaaaacc gatggcacgc aacgcaaaat attacaatca tgataattcg accgttcttg
     1621 cccacacgca cgagcggtat tctcacgcat ttaagtcaga ctggtaccag catccctcat
     1681 gcactgaaga acaggccgaa tggcttattc agtgttaccg caggcgcgga tgcgaggtta
     1741 aaaaagccct tagcctcgac taccgtcact agataatctc cgtcaggctc ccttactccg
     1801 aacagccacc gcgtccgtcc cgcacattcc agcaacggat ctggaggtaa cgtgcgggta
     1861 ttacttcgac ctgttctggt tccggaactc gggctggtgg tccttaagcc cggtcgtgaa
     1921 tccatgccgg tattccacaa tacccgggta ctggtggagc cggaaccgaa aagcatgcgt
     1981 aatctgccgt ccggggtcgt tcctgccgtt cgccagccgc tagtggaaga caaaacattg
     2041 ctgccgtttt tcagtaacgc acgggtgatt cgtgctgctg gtggtgctgg cgcattgtct
     2101 gactggctgt tgcgccatat taaatcctgc cagtggccac acggcgatta tcatcacagc
     2161 gaaaccgtca ttcaccgtta tggtaccggc gcaatggtat tgtgctggca ctgcgacaac
     2221 cagctgcgtg accagacatc cgaatcactc gagcaacttg ctcatcaaaa cctgtcagca
     2281 tggatgattg acgtcatcgg tcacgcaata agcggtacgc aggagcgtga attatctctg
     2341 gctgaattat cctggtgggc ggtccgcaat caggtggcgg acgcgctacc ggaagcggta
     2401 ttacgtcgtt cgctggggtt gcgtgcggaa aaaatccgct caatgtaccg tgaaagcgac
     2461 atcgtgccgg gagagcagac cgccaccagc atactgaagc agcgcacaaa aaatcttgcg
     2521 ccgctgcctc acgcccacca gcaacagaat ccaccacagg aagagacggt ggtcagcatt
     2581 gccgttgatc cggaatctcc ggctcagtat ctccagcgcc agaaaccaca acgggaagag
     2641 atgcctgtat acacgcgctg ggtaaaaacg cagaaatgca tgacgtgtgg caatcaggca
     2701 gatgatccgc accacatcat tggtcatgga ctgggaggga tgggaacaaa ggctgacgat
     2761 ttgtttgtta ttccgctgtg tcgtaaatgc cataacgaac tacacgccgg ggtaaaagat
     2821 tttgaagaaa aacacggtag tcagttgttg ttgctgattc gttttttaat gcacgcgaga
     2881 aattcgggtg ttctgaagtg gaaagcataa atgaccgaac gcatagaatt tgttttgcct
     2941 tacccgccaa cggtgaacac ctactggcga cgtcgtggca gcacatattt tgtatcaaaa
     3001 gccggtgagc gttatcgccg tgatgtggcg ctaattgttc gccagcagcg actgaaatta
     3061 aacctgtccg gaaggctggc gataaagatt attgcagagc caccggataa gcgccgtcgt
     3121 gacctggaca atatcctgaa ggcaccgctg gatgcgctga cgcatgctgg actacttata
     3181 gacgacgagc agtttgatga aatcaatatt gtgcgcggtc agctcgttcc tggtgggcgg
     3241 ctggggataa aaatcacaga actggggtgc gcatgaataa ccagtattta cagtttgtgc
     3301 gtgagcagct cattatcgcc accgctgatt tgagtggggc aacaaaaggt cagcttgaag
     3361 cctggcaaga gaatgccatg ttcgatacag ggcgttacag gcgtaaaaaa atccggtacc
     3421 gcgatgaagt gactggaaaa atgataacgc gggataatcc accaatcccg ggaaagcaat
     3481 cgctggcgaa ggggacgtca attcctctgg tcagtccggt tgagttttcg acatcatcgt
     3541 ggcggcgggc tgttctgtct cttgaagagc actataaagc ctggttgttg tggtgttaca
     3601 gcgggagtat ttgttgggaa tatcagatcg cgataacaca gtgggcgtgg aatgaattta
     3661 atgctcaatc cggtaccaga aaaattgcag ggaaaacgca ggaacgcctg aaaaaattaa
     3721 tctggctggc ggcgcaggca gtaaaagcag aactttttgg tggggaaggt tatgaatacc
     3781 aggagctggc attactggcg ggagtgacaa ctaaaaactg gtccaaaaca tttactcgtc
     3841 actgggttgc aatgaaacac atttttcacc gactggatag tgaggcttta ttgtttgtaa
     3901 tgagaacacg ttcaaaaaaa agacggcatt ttcaaagcaa agtgttgcaa aagtagattg
     3961 aaaggcatat attcatgcaa atctgatact ttgccgattt tgtacgtgat ggcaaaagca
     4021 aacaaaaccc gcccacaagc gggttttttg tgccacttat ctcggataga catggtgaat
     4081 gcgctggtgg aggagataag ggtgattttt gaatgcttgc aacattgatt tcgtaacgtt
     4141 attatcctgc gcccggccct ttagctcagt ggtgagagcg agcgactcat aatcgccagg
     4201 tcgctggttc aaatccagca agggccacca accgccacta gctcatcagg aaagagcgtc
     4261 aaccctttaa gttgagtgtg cgaggttcga gtccccggtg gcggtccagt gccgacttgg
     4321 ctcagtagg
//
LOCUS       DAJTSA010000194         4209 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000194 DAJTSA010000000
VERSION     DAJTSA010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.195"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO369_004052"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO369_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5984109.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OO369_004053"
     tRNA            complement(324..399)
                     /locus_tag="OO369_004053"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OO369_004054"
     tRNA            complement(406..480)
                     /locus_tag="OO369_004054"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OO369_004055"
     tRNA            complement(597..681)
                     /locus_tag="OO369_004055"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OO369_004056"
     tRNA            complement(690..765)
                     /locus_tag="OO369_004056"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OO369_004057"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OO369_004057"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR5984110.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO369_004058"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO369_004058"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR5984111.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO369_004059"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO369_004059"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR5984112.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1089 a         1046 c          937 g         1137 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggctt
//
LOCUS       DAJTSA010000195         4198 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000195 DAJTSA010000000
VERSION     DAJTSA010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.196"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            84..752
                     /locus_tag="OO369_004060"
     CDS             84..752
                     /locus_tag="OO369_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984113.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(995..1690)
                     /locus_tag="OO369_004061"
     CDS             complement(995..1690)
                     /locus_tag="OO369_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR5984114.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRPEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1683..3110)
                     /locus_tag="OO369_004062"
     CDS             complement(1683..3110)
                     /locus_tag="OO369_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR5984115.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3121..3840)
                     /locus_tag="OO369_004063"
     CDS             complement(3121..3840)
                     /locus_tag="OO369_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR5984116.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1118 a         1085 c          870 g         1125 t
ORIGIN      
        1 ttgctgatat ttaacccgaa cattcctgat acagactatt aatacaacat tatctgcgac
       61 agattttatt ttctggatat attatgagtg aacaaatcaa acaggatatc gatctgattg
      121 agattttatt tcatctgaag aaaaagattc gtgttatcct ttttattatg gctatctgta
      181 tggcaatggt gctgttgttt ctgtatatca ataaagacaa tacaaaagtg atttacagcc
      241 ttaaaataaa ccagacaacg ccaggtatac ttgttagctg tgatagcaat aataattttg
      301 cctgtcagac cacaatgact gaagatgtta ttcagcgaat tactacattt tttcagacca
      361 gcccagatat caagaacaga gaaataaggc tggaatggtc aggagataag agagatttac
      421 caactgctga agaggaaata tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag
      481 aataccataa tggcaggcaa gttctcgatg agatacaaac gccttcagca attaacagtg
      541 agctttatac aaaaatgata tacctgacca ggaactggtc attgtatccg aacggtgatg
      601 gctgtgtaac tataagctca ccagaaataa aaaataaata ccctgctgcc atttgcctgg
      661 ctctgggatt ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc aaaaaaatgg
      721 tagatgaata ccaacaaaac tctgggcagt aataaacagc attctgatag agagtgcctg
      781 tataaagctg aagtgacaat gaccgggaag ggaacccgtt ttcaggaatg gatgcctgtc
      841 tttttcactg taaatatcct ggcattacta atgcctggat atttacagaa gagcagcata
      901 accatgacgg aacggggtta agtcaggttg tccttaacgc tattttcaac ctcgtatgcc
      961 ttcttcaggt ttatgttcag atctcatatc tctctcagca atcctcaata atcagataca
     1021 ccgctttcac cggaccatga accccgacga ctttgataag ctcaatatcc gccgttgagc
     1081 tgggaccact gatgatgtta atacatgacg gcattcgttc accggcctgc gctttctgat
     1141 gcaatttttc tgcgagttgc gctacacgcg gcaggattgt gcttttacgc aggataaaaa
     1201 gagaagattc cgggagcagg ctcaatgaac gcccgcgctc ggcggcggaa aaaagaacca
     1261 cgcctcccga ttcggttaaa ccatattcag catacacgac acccacttta gcctgctctg
     1321 cctgcgagat attctcggca cctttcgccg gatcccaaac aacggcattg cattcctgct
     1381 gcaaacgttc actaatcccc aattcctccg gcctcgtgtc accgctaatc acgaccgact
     1441 gatctcccag ctctttacac agacgtattg cagcttctgc cgccttcgcc tcgctggtca
     1501 gctcacagcg cgtcaacata acatcgctgg caaactgaat aaacgcgtca cagcgctgct
     1561 gttggttaag ttgggtaagc cgctcgttag catagttgtt aagcggcgca tcttctgctt
     1621 gcggttcaag tcgcagcggg cgacccagtg cctgagcaac gttattcaaa aattcgctcc
     1681 gattatccat tctttttctc ctgcgcctga tgtttcttaa accaactacg gaaactctct
     1741 ccgtcagctt caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt
     1801 ggtgttttgc cgccattgat aaaccagctt gccgcatgag cgccggccat catcccgact
     1861 ttccacaatc ctggatgact attggcataa gcgaacattt ttatcgcccg ttgctctgct
     1921 tttgcggtga tccctttttc agccatcacc cgacgatgac gcaaaatcag ttttgacagc
     1981 ggaatacgca ccggacacac gctgtcacaa gcagtgcata aagagcaggc gtagggtaaa
     2041 tctttaaaat ctttatagcc gccaagtagc ggagaaatca ccgcaccaat tggccctgga
     2101 taaatagagc catatccatg accgccaata tggcgatatg ccggacaagt attcatacaa
     2161 gccccgcagc gaatacagcg cagcacatcc cgaaattcag aggccagcac ctcagaacgc
     2221 ccgttatcga caataaccag atgaaactct tcaggaccat caacgtgccc cgcttcgcgc
     2281 ggccctgtca gccaggtgtt gtatcccgtc aaacgtgcac caacggcact gcgcgccagc
     2341 atggtgatca atacatctac ctcggcaaac gtgggggcaa tacgctccat tcccatcact
     2401 gcaatatgcg ttttaggcag cgtggtacac attcgcgcat taccttcatt ggtcaccagg
     2461 cataccgaac cggtctctgc caccgcgaaa ttacagccgg taatacctat ttcagcactg
     2521 aggaaatctt cacggatttt ttgccggatg aataaggtca tcgcttcagg cgtttccggc
     2581 ccctcatagc ccagacgttc gtgtagcact cgacggatct gatggcgatc tttatgaatt
     2641 gccgggacca caacatgaga tggcggatct tgatccagct ggagaatata ttcacccaga
     2701 tcggtttcaa tcacctgaat gccagcatcc tgcaacacat gattgacacc aatctcttcg
     2761 gtcaccatcg atttagattt caccaccttc cgggcatttt tgcgttgggc aacctgtaaa
     2821 atgtagcggg tagcgtcttc tttggttttt gcaaaataga cgtgaccgcc gttttgcgtc
     2881 actttttctg agagctggta cagataagcg tcgagattac tcagaacatg atcacgtatc
     2941 tgggcggccc gatcgcgcca ctcctcccag tgccccaatt catcgaccat tttttgccga
     3001 tttgccccaa tacgctgctg cgcgcttgcc accgctttgc gcatgatcgg atcttcaatt
     3061 tgctgacgga tgcgtgtctt aaaatctgta ttactggttt tgatcgacat ctttatatcc
     3121 tcagcggctc atcaacactt cagcaatatg catcactttg actttctgcc cttcccgttg
     3181 taatcgccca ctgatgttca gcaggcaact cacgtcagca ccaattaaat actcagggcg
     3241 gacttccatc aggtgcacaa ccttttcttt caccatctcg ccggatattt cggccatttt
     3301 gaccgagaac gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg tcaacagctc
     3361 cagtccacgc acatttttca gcagcgtaag tggctcgtcc ttcactccca gcttacgggc
     3421 caggctacaa gatgggtgat acacagctct cccttgcaaa ctggcaccta catcgactac
     3481 ccctaattta ttaacaataa aagaggtgag atcctgcata cgcgcggcaa ccttttcggc
     3541 acgtgatgcc cattcaggtt catccgccag atacgtcggg taacttttta tggcataggt
     3601 gcaagagcca gccggtgaaa taatgggatc gtcgttatcc tccagtgcgg cgatcagatt
     3661 tttcatccct ggaatcgctt ctttgatata accgctattg atcgcaggct gaccgcagca
     3721 tccctgtttc tccgggaaat ttacgcgaca gccgagtttt tccagtagca gcacggagtc
     3781 tcgtgccatt cttgatttca gggcgtcacc aatacaggtg acaaagaaat tgacattcac
     3841 aactaatact ccattacttc atgcccattt atgcccgatg tttccccccc accgggggat
     3901 caatcatcaa taaatatggc gttctttttt aattggctag caacatacca cccctcattt
     3961 gtatgacaaa tgataatctt tcatccaaac ttcaaatgca ataactaatt ttatttcttt
     4021 cggcatcgct cacattttat ggggaaatcg acgatcattt ttggcataag aaatccattt
     4081 aaaaatggct acatccctta tacctattca gagctactta ttcaggggtt attgagatat
     4141 acactgatgg tgtacaaaaa aatcaccgac aataatcacg tatcaagtaa tagtcgga
//
LOCUS       DAJTSA010000196         4183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000196 DAJTSA010000000
VERSION     DAJTSA010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.197"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(348..995)
                     /gene="ychE"
                     /locus_tag="OO369_004064"
     CDS             complement(348..995)
                     /gene="ychE"
                     /locus_tag="OO369_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR5984117.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1472..4147
                     /gene="adhE"
                     /locus_tag="OO369_004065"
                     /gene_synonym="adhC"
     CDS             1472..4147
                     /gene="adhE"
                     /locus_tag="OO369_004065"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR5984118.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEDETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1168 a         1021 c          960 g         1034 t
ORIGIN      
        1 aattcaatca aatagagatt ggctaagcat gtctatcttt tctgattaca gttccagtag
       61 tgaaatgcac aataatttaa caattgatta ttatttagca ctttcctcta ccaaaggcag
      121 tggaattact aatatcattt caattattct tcagcaagcc caggattatg atgtggcgaa
      181 aataacatga tggtgaggag aagcagttaa ggaaatcctt ataactaact gtaatttgag
      241 aagaatattt ttacctattc cttttgcttt tgataactgc gcgggaacaa agtaaaaata
      301 atgacgaagt aaacggagca tggcagctcc gtttcatttg aaaggaatta attaagcagg
      361 ccggggaaaa tccccttaat accagtaacg ataaattcaa tccccaatgc catcagcaat
      421 agccccataa tacgcgtaat cacgttgatg ccggtctggc gtaaaacccg taccagccac
      481 ggtgccatgc ggaacaatcc ccagcaacat aaagcgaaca atgcaatagc cacaaagaaa
      541 ccaaacagat agctaatgct gtgataacgc gtaccccaga cgatggtaga actgatcgcc
      601 cctggccccg ccatcaacgg caacgccaat ggcaccacac caatactttc acgtaccgcg
      661 gtttctgatt tttcttgctt gttctgttta tcctcgccaa gcttgccgct gatcatcgac
      721 atcgctatcg tcaccaccag gatgcccccg gcgatacgga acgaatcaat tgatatacca
      781 aaaagttgta gaatcgtgtc gccgagaaaa agcgagatcc acaagataat ggccacagac
      841 aggttggctg taaggttagt tttgtttcgc gctgccgctg tctgataact ggtcatgctg
      901 ataaagacgg gaataatccc taccgggttg accagcgcaa ataacccgat gaaaaatttg
      961 aagtaaacgg gaaaatcaaa aagggtctga atcacggttc gctccgaagc aaaagccgga
     1021 taatgttagc cataaataag gttgaaaaga cgcgctgaca atacgccttt tgacagcatt
     1081 tttcacctcc taactactta aaattgctat cattcgttat tgttatctag ttgtgcaaaa
     1141 catgctaatg tagccaccaa atcatactac aatttattaa ctgttagcta taatggcgaa
     1201 aagcgatgct gaaaggtgtc agctttgcaa aaatttgatt tggatcacgt aatcagtacc
     1261 cagaagtgag taatcttgct tacgccacct ggaagtgacg cattagagat aataactcta
     1321 atgtttaaac tcttttagta aatcacagtg agtgtgagcg cgagtaagct tttgattttc
     1381 atcggttaag caaatcatca ccgcactgac tatactctcg tattcgagca gatgatttac
     1441 taaaaaagtt taacattatc aggagagcat tatggctgtt actaatgtcg ctgaacttaa
     1501 cgcactcgta gagcgtgtaa aaaaagccca gcgtgaatat gccagtttca ctcaagagca
     1561 agtagacaaa atcttccgcg ccgccgctct ggctgctgca gatgctcgaa tcccactcgc
     1621 gaaaatggcc gttgccgaat ccggcatggg tatcgtcgaa gataaagtga tcaaaaacca
     1681 ctttgcttct gaatatatct acaacgccta taaagatgaa aaaacctgtg gtgttctgtc
     1741 tgaagacgac acttttggta ccatcactat cgctgaacca atcggtatta tttgcggtat
     1801 cgttccgacc actaacccga cttcaactgc tatcttcaaa tcgctgatca gcctgaagac
     1861 ccgtaacgcc attatcttct ccccgcaccc gcgtgcaaaa gatgcaacca acaaagcggc
     1921 tgatatcgtt ctgcaggctg ctatcgctgc cggtgctccg aaagatctga tcggctggat
     1981 cgatcaacct tctgttgaac tgtctaacgc actgatgcac cacccagaca tcaacctgat
     2041 cctcgcgact ggtggtccgg gcatggttaa agccgcatac agctccggta aaccagctat
     2101 cggtgtaggc gcgggtaaca ctccagttgt tattgatgaa actgctgata tcaaacgtgc
     2161 agttgcatct gtactgatgt ccaaaacctt cgacaacggc gtaatctgtg cttctgaaca
     2221 gtctgttgtt gttgttgact ctgtttatga cgcagtacgt gaacgtttcg caacccacgg
     2281 cggctatctg ttgcagggta aagagctgaa agctgttcag gatgttatcc tgaaaaatgg
     2341 tgcgctgaac gcggctatcg ttggtcagcc agcctataaa attgctgaac tggcaggctt
     2401 ctctgtacca gaaaacacca agattctgat cggtgaagtg accgttgttg atgaaagcga
     2461 accgttcgca catgaaaaac tgtccccgac tctggcaatg taccgcgcta aagatttcga
     2521 agacgcggta gaaaaagcag agaaactggt tgctatgggc ggtatcggtc atacctcttg
     2581 cctgtacact gaccaggata accaaccggc tcgcgtttct tacttcggtc agaaaatgaa
     2641 aacagctcgt atcctgatta acaccccggc ttctcagggt ggtatcggtg acctgtataa
     2701 cttcaaactc gcaccttccc tgactctggg ttgtggttcc tggggtggta actccatctc
     2761 tgaaaacgtt ggtccgaaac acctgatcaa caagaaaacc gttgctaagc gagctgaaaa
     2821 catgttgtgg cacaaacttc cgaaatctat ctacttccgc cgtggctccc tgccaatcgc
     2881 gctggatgaa gtgattactg atggccacaa acgtgcgctc atcgtgactg accgcttcct
     2941 gttcaacaat ggttatgctg atcagatcac ttccgtactg aaagcagcag gcgttgaaac
     3001 tgaagtcttc ttcgaagtag aagcggaccc gaccctgagc atcgttcgta aaggtgcaga
     3061 actggcaaac tccttcaaac cagacgtgat tatcgcgctg ggtggtggtt ccccgatgga
     3121 cgccgcgaag atcatgtggg ttatgtacga acatccggaa actcacttcg aagagctggc
     3181 gctgcgcttt atggatatcc gtaaacgtat ctacaagttc ccgaaaatgg gcgtgaaagc
     3241 gaaaatgatc gctgtcacca ccacttctgg tacaggttct gaagtcactc cgtttgcggt
     3301 tgtaactgac gacgctactg gtcagaaata cccgctggcg gactatgcgc tgaccccgga
     3361 tatggcgatt gtcgacgcca acctggttat ggacatgccg aagtccctgt gtgctttcgg
     3421 tggtctggac gcagtaactc acgccatgga agcttatgtt tctgtactgg catctgagtt
     3481 ctctgatggt caggctctgc aggcactgaa actgctgaaa gaatatctgc cagcgtccta
     3541 ccacgaaggg tctaaaaatc cggtagcgcg tgaacgtgtt cacagtgcag caactatcgc
     3601 gggtatcgca tttgcgaacg ccttcctggg tgtatgtcac tcaatggcgc acaaactggg
     3661 ttcccagttc catattccgc acggtctggc aaacgccctg ctgatttgta acgttattcg
     3721 ctacaatgcg aacgacaacc cgaccaagca gactgcattc agccagtatg accgtccgca
     3781 ggctcgccgt cgttatgctg aaattgccga ccacctgggt ctgagcgcac cgggcgaccg
     3841 tactgctgct aagatcgaga aactgctggc atggctggaa acgctgaaag ctgaactggg
     3901 tattccgaaa tctatccgtg aagctggcgt tcaggaagca gacttcctgg cgaacgtgga
     3961 taaactgtct gaagatgcat tcgatgacca gtgcaccggc gctaacccgc gttacccgct
     4021 gatctccgag ctgaaacaga ttctgctgga tacctactac ggtcgtgatt atgtagaaga
     4081 tgaaactgca gcgaaaaaag aagccgctcc ggctaaagct gagaaaaaag cgaaaaaatc
     4141 cgcttaatca gtagcgctgt ctggcaatat aaacggcccc ttc
//
LOCUS       DAJTSA010000197         4182 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000197 DAJTSA010000000
VERSION     DAJTSA010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.198"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..186)
                     /locus_tag="OO369_004066"
     CDS             complement(<1..186)
                     /locus_tag="OO369_004066"
                     /inference="COORDINATES: protein motif:HMM:NF017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCR5984119.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKK"
     gene            complement(327..2360)
                     /gene="metG"
                     /locus_tag="OO369_004067"
     CDS             complement(327..2360)
                     /gene="metG"
                     /locus_tag="OO369_004067"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR5984120.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2492..3601
                     /gene="apbC"
                     /locus_tag="OO369_004068"
     CDS             2492..3601
                     /gene="apbC"
                     /locus_tag="OO369_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR5984121.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3863..4144
                     /locus_tag="OO369_004069"
     CDS             3863..4144
                     /locus_tag="OO369_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR5984122.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1016 a         1044 c         1062 g         1060 t
ORIGIN      
        1 cttcttcacc ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga
       61 aaaacttttt atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc
      121 gtttccctgt tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg
      181 tctcattctg catccctata attttatcca gttgaatgca gatgctacca gtatttatac
      241 gggttagaga gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc
      301 tgtcgatgca gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca
      361 tccgggctta gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca
      421 gagataccga agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca
      481 atcagtgcct gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca
      541 ccgccgagat ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt
      601 tcgatcagcg ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga
      661 atcggatcat ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta
      721 gaggcttcca ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac
      781 ggattcactt tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg
      841 aggaaggctt ctgcacgctc ggtcagtttc ggcagtacag gcttcaggta agtcatcagc
      901 acgcggaaca ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc
      961 tgttttgcga ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt
     1021 gccatgattt cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact
     1081 tcagcggcat cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca
     1141 ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc
     1201 acgatatcgg cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg
     1261 cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc
     1321 caggtgctgg ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc
     1381 acatagccat gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca
     1441 ggccagaaca ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg
     1501 gtggagtctt tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga
     1561 ttcttgaaag aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc
     1621 ggtgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc
     1681 aggccggatt caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg
     1741 cgggtccatg cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa
     1801 tcacgcatca ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg
     1861 ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc
     1921 gggcaggtgc ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc
     1981 tgagagatgg tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct
     2041 gacaactggc ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag
     2101 cctgcgaaat cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata
     2161 ccgagctgct gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag
     2221 ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc
     2281 agcatatggc cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt
     2341 ttcttcgcga cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag
     2401 taccaaatgg gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct
     2461 gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc
     2521 agaagccttg cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa
     2581 gcataacctc accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca
     2641 tgtggaactg gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg
     2701 tagcgccgaa ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa
     2761 catagccact ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat
     2821 cgcggtgagt tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct
     2881 ggcactggcg gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc
     2941 aattcccacc atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat
     3001 ggcaccgatt atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga
     3061 caatgcgatg gtgtggcgtg gaccgatggc gggcaaggcg ttaatgcaga tgttgcagga
     3121 aaccttgtgg ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat
     3181 tcaattgacg ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca
     3241 ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc
     3301 ggtgctcggt attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga
     3361 accgattttc ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct
     3421 ggggcagatg ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt
     3481 tatcagccgt ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc
     3541 agctcagctc tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta
     3601 acgcctatta accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt
     3661 gtatttgttt ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct
     3721 tcgtccgaaa ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg
     3781 tgctgtgtag tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc
     3841 tttactacag ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc
     3901 ctatggattg gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag
     3961 gatcagtccc ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg
     4021 tcagttaacg tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac
     4081 gacagaagtg gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga
     4141 ttaatttggc aacctgattt taattgcatt tagaaagacg aa
//
LOCUS       DAJTSA010000198         4168 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000198 DAJTSA010000000
VERSION     DAJTSA010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.199"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..334)
                     /gene="flhe"
                     /locus_tag="OO369_004070"
     CDS             complement(<1..334)
                     /gene="flhe"
                     /locus_tag="OO369_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCR5984123.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(334..2411)
                     /gene="flhA"
                     /locus_tag="OO369_004071"
                     /pseudo
     CDS             complement(334..2411)
                     /gene="flhA"
                     /locus_tag="OO369_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="OO369_004072"
                     /pseudo
     CDS             complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="OO369_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            3787..>4168
                     /locus_tag="OO369_004073"
     CDS             3787..>4168
                     /locus_tag="OO369_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR5984124.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT         1038 a         1208 c         1021 g          901 t
ORIGIN      
        1 caccgcctgg cacttcccag ataaatcgca acggttctgc tgccgctata ccggaaaagg
       61 ccacggtggt accgctctgc ccctctaatt cgacacaacg agattgcgaa cacaagcgaa
      121 cccgcagtcc tgcaggtgtc gggccgataa gctgataacg ccacgccacc agcgtcatca
      181 atcctgaagc aggttgtcgc gcagaaagag gtgcagacga catcgactca ccgcgatgat
      241 ttagcgtaat tccgacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca
      301 gcggaaacaa taatatcgct aataaggttc tcatcattta ccgccaattg tcgccgtcat
      361 gcggatatgt cggttatcag acagttccag attcgacagg accactaact gcggcaagct
      421 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca atactggcgg
      481 cgcacccagc atctcctgac gggatagagc ttcctgagtt tgcgccagta aacgatccgc
      541 cagccctggc tccagtcctc ccccgccctg cagcgcctgt agtaacaaac gttccagcgg
      601 tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc
      661 ccgtcccaac gccacgcgca cgacggcgct taattcatgt ggatcgcttt ggatgggcgc
      721 atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggtactt tttcatcgag
      781 gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggaa cgagatcttc
      841 cgtcagcttt ggcatctcct gggcgacgcg atccaacagc tgttgcgcct cctgacgacc
      901 aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct
      961 ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac tttcaatcca
     1021 gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc
     1081 ggttccaggg ttaatcgcca gccagcgccc cggataagca tcaccactgc caatctccac
     1141 gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac
     1201 cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc gtatacggcc
     1261 caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac ccacttccat
     1321 tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg tattattctc
     1381 tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc gtatccacca
     1441 ggccagcccc agcaatccgg cagtgaacag caaaaatacc aggttcggca ttccaggcac
     1501 caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt tactgaaaag
     1561 ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa cgatgccccc
     1621 cgcggcggta gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc caatggtcaa
     1681 tagcgtataa ctttccgccg cgtgtcccat gctcatgcca gttgcagcac gccgaccagc
     1741 aacccgccga caacgttaat gaccatgatg aggatcccgg caatggcatc gccgcgaaca
     1801 aacttacttg ccccgtccat tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg
     1861 cgttttttcg cctcatcttc accaatcaat ccagcgttaa ggtcggcgtc aatcgccatc
     1921 tgcttacccg gcataccatc gagaacaaag cgcgcaccca cttctgctat acgcccggca
     1981 cctttggtaa tgaccataaa gttgatgatc acgagaatga caaacaccac gataccgata
     2041 gcgaaattgc caccaacgag gaagtgaccg aacgcttcga ccaccttccc agccgccgcc
     2101 gcgccggtat gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc
     2161 aacaacatag taaacaacag aatggtcgga aacgcagcaa actcaagcgt gcgctgggta
     2221 aacatcgcca ccagcaacac catgatcgac aaggcaatat tgaaggtaaa caacaggtcg
     2281 agtatgaatg cgggcagtgg cagcaccatc atcgacaaga tcaacaggat caaaatcggt
     2341 ccggcaagga tctgccatta tgtcgatttc aggtttgcgg gcaggcgcag catcgcggcc
     2401 agattactca tgggtcggtt tctcgttaat aaaatccagg gctgccggca ccggaagatg
     2461 agtaggtgta cagggcgctg tccaccagcc agacgccagc gtttcagttg ccagacccag
     2521 gccagcactt ccgccaccgc ggcgtacagt tgacccggga tttgttgacc aatctccgca
     2581 tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg tcgggacgtt attttcagcg
     2641 ccaatttcac gaatgcgcag cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg
     2701 ctcattttgt tttcgtcata ctgcaacgct accgaatagt gggtcgggtt attgacaatg
     2761 acatccgctt tcggcacatc ggccatcatc cgacgccgtg cggcagctcg ctgcatctga
     2821 cagatccgtc ctttaacatg ggggtcgcct tcgctttgtt tgaactcatc acgaatatcc
     2881 tgccgcgaca ttcgcagctt tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc
     2941 accattggaa tgacaccaag caccaccagc agtgcgcata gccctaccaa atccatcgca
     3001 ttacccatgg cggtaatcgg tgactcggcc attaagcgca tcatctgcgg ccagtgatgc
     3061 cagagaaaaa accccgtcac gctgccaacc aggatggttt tcaaaattgc cttaagcaac
     3121 tccgcgctag tctgtgccga gaatatccgt ttaatgcccg gtagcgggtt gagtttggaa
     3181 aacttcggct gcaaggattt gccgctaaat accagccccc ccagcataac cggagaaatg
     3241 agcgccacca gcaccacgcc gctaatcagc ggtagcagcg ccagcatggc ttctctgatc
     3301 agcagaataa tttgcccgag gatcagattc gggtctttga taatactgtg atcaaaatgc
     3361 agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc aaaccagata
     3421 acactaacgc ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg cgggatttgc
     3481 ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc
     3541 tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg taaatgatgc cagagcgcaa
     3601 agcgttcaat ggtttaagta aggggcaaaa caggcgggat ttagggcgtt tatgcaaaaa
     3661 taggttcagt tgcagaacgt tttgcgaacc attttccagt tctgatattt agccatttag
     3721 cctatagaat cgcactccgc ctctctgtta gtttgctaat aattaaatta ttacaaaggt
     3781 gatcaaatgg aatggcaaca attgcttaac tgcaaccgtc ggaaagataa tctaaaaaaa
     3841 accaacatag catttttaca tccccttgct agcggaagac aagagatcga gcgggattac
     3901 gaccgattac tttttgctgc accaactcgt cggcttgcgg ataaaacaca agttttcccg
     3961 ttagatcaaa atgacagcat acggacgagg ctgactcatt ctcacgaagt agcaaacttt
     4021 gctcgcggga taggaatgag gctcgctttc gagatgaagg agcacatctt tggcgaaata
     4081 cccacgggga ttgtcgttga acgcgacgtt cctgctctgt tagcagcgat cggactggca
     4141 cacgatctcg gtaatccacc atttgggc
//
LOCUS       DAJTSA010000199         4114 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000199 DAJTSA010000000
VERSION     DAJTSA010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.200"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..158)
                     /locus_tag="OO369_004074"
     CDS             complement(<1..158)
                     /locus_tag="OO369_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5984125.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="OO369_004075"
     CDS             complement(308..469)
                     /locus_tag="OO369_004075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984126.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(466..654)
                     /locus_tag="OO369_004076"
     CDS             complement(466..654)
                     /locus_tag="OO369_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR5984127.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            914..1249
                     /gene="iraM"
                     /locus_tag="OO369_004077"
     CDS             914..1249
                     /gene="iraM"
                     /locus_tag="OO369_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR5984128.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1530..1638)
                     /locus_tag="OO369_004078"
                     /pseudo
     CDS             complement(1530..1638)
                     /locus_tag="OO369_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2161..2237)
                     /locus_tag="OO369_004079"
     tRNA            complement(2161..2237)
                     /locus_tag="OO369_004079"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Arg,seq:tct)
     gene            complement(2339..2414)
                     /locus_tag="OO369_004080"
     tRNA            complement(2339..2414)
                     /locus_tag="OO369_004080"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2379..2381),aa:Ile,seq:cat)
     gene            complement(2559..3380)
                     /locus_tag="OO369_004081"
     CDS             complement(2559..3380)
                     /locus_tag="OO369_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5984129.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3395..3751)
                     /locus_tag="OO369_004082"
     CDS             complement(3395..3751)
                     /locus_tag="OO369_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5984130.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3764..>4114)
                     /locus_tag="OO369_004083"
     CDS             complement(3764..>4114)
                     /locus_tag="OO369_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5984131.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT         1155 a         1118 c          793 g         1048 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      781 gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgt
      841 tctatggttt aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      901 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      961 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
     1021 cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt
     1081 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa
     1141 aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
     1201 ttgcactctg caaatatgcc cttatggact aaaacaacaa agtccataag gagtttactc
     1261 acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
     1321 tgccattcca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
     1381 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
     1441 tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt
     1501 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
     1561 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc
     1621 cggctgctgc aatgccatta acaaaaacag taagcaatca ctttttaccg taacaggtga
     1681 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
     1741 tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact
     1801 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac
     1861 gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga
     1921 ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac
     1981 ccacaccata tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat
     2041 actcaatcaa tacttaacag aacttttatt tttgacaaat atataatatt ttcaacaata
     2101 tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata
     2161 tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     2221 ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc gccccccgca
     2281 ccaacaaccc tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg
     2341 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     2401 tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaatttcgc aataaaaaaa
     2461 cccgctcagc ggcgggttgt agaaactctt ctaacgccag gcataaaacg cccatcatta
     2521 tgacgaattt accacagatt ccggaaaaat caaccttgtt acctagttac cttttttaac
     2581 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc ataaaatttc
     2641 ttaactgttt tttcccatga cgcgcgtgtt atctggtttg tcacctcgca tatagcatta
     2701 aatgcctccg ttgatggtag tctttcatag ccacgaccac cacaacgctg gcagtctctg
     2761 ataacaggca taccacgttt taccgactct tcacgatgaa tggcgacacc acgcccacgg
     2821 caatccttac aggcggtgga aacctcaccc tttccgccac actccggaca ggcaactttt
     2881 accacctccc tgactttttt ccattcttcc cagtaaaacg gatacacacc tttcgtacac
     2941 tttgcccata ccggcggctt accatccgga tactggatct tgtttgtaaa aactatgctt
     3001 tcaataaatt tttccccata gcaacaaggg cactgctttt tactcgctgc gctgcgggca
     3061 taatcctcaa aagcgtacga agccataatg cgcatcacta ccggttttat ttctgccgga
     3121 agttttctca acgccgccac acgatcgcac cgactgagtg cataatctgc cagtaattct
     3181 gttgcccgcg ccctgtcatt catactgatg cccattttcc ccaggaacgc agaaaaaccc
     3241 atctcagccc gattctgtgt catgccctgc gcggccatca catcagtgat actcagcgca
     3301 tcttttgacg ttgaggccga tgcatcggtc aggccagggg attttgggga gtagtatttc
     3361 ggtaaatctt ccagtttcat tttttgacct gctcttaatg cattatgggg taaatcttca
     3421 cccccagacg tccaccagat actggctgac cacgaacgat attgatttca tcaaactgct
     3481 catcgtccat taacactccc gcatgcgtca gcgcatccag cggtgctttc aggatattgt
     3541 ccaggtcgcg acgacgctta tccggtggct ctgcaatcac ctttatcgcc agccttccgg
     3601 acaggcttaa tttcagccgc tgctggcgaa caataagcgc cacagcccgg cgataacgct
     3661 ttccctcctc cgagataaaa tatgtgctgc cacggcgtcg ccagtaagtg ttcaccgtcg
     3721 gcgggtaagg taaaaccaaa tctatgagca tcagtcacct cttttaccca agcacgccag
     3781 ttgcaaaggc gtgatcaaga aaacgaaaaa ttaaatcaac ctgagaacca tgcttttctt
     3841 cgaacgccag aggatccgca tgaagctcgt tgtgatgctc ccgacacagc ggtagcgtga
     3901 aaatatcgtg agatttggtc cccattccgc cctgaccatg accaatcagg tgatggggat
     3961 cgtcggctgg cttaccacaa cacgcacacg gctgtgtctt cacccagcgt ttgtatttct
     4021 cgttaaccca gcggcgacgt ttaggtcgtt tcatgaaaga ttccggagac tcaggatcaa
     4081 cggcaatgct gaccaccgtc ttttcctgtt gtgg
//
LOCUS       DAJTSA010000200         4062 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000200 DAJTSA010000000
VERSION     DAJTSA010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.201"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(906..1097)
                     /locus_tag="OO369_004084"
     CDS             complement(906..1097)
                     /locus_tag="OO369_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5984132.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENL"
     gene            1162..1485
                     /locus_tag="OO369_004085"
     CDS             1162..1485
                     /locus_tag="OO369_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR5984133.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1427..2185
                     /locus_tag="OO369_004086"
                     /pseudo
     CDS             1427..2185
                     /locus_tag="OO369_004086"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2163..2693
                     /locus_tag="OO369_004087"
     CDS             2163..2693
                     /locus_tag="OO369_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR5984134.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2700..2933
                     /locus_tag="OO369_004088"
     CDS             2700..2933
                     /locus_tag="OO369_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR5984135.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3479..3871)
                     /locus_tag="OO369_004089"
     CDS             complement(3479..3871)
                     /locus_tag="OO369_004089"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984136.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          969 a          925 c         1215 g          953 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacgc gccctgccgg gagcagacaa
      901 gcccgtcaga ggttttcacc gtcatccccg aagcgagcga ggcaactgcg gtaaagctca
      961 tcggcgtggt cgtaaagcga ttcacagatg tctgcctgtt catccgcgtc cagttcgttg
     1021 agcttctcca gcagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt
     1081 ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg
     1141 ataccgatga aacgcgagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
     1201 ttactggaac gctgtgaggg taaacagctg gcggtatgga tgcggcgggt ctgcctgggt
     1261 gagccggttg cccgttccgg gaggctgccg acactggcac cgccgttact gcgtcagctg
     1321 gccgccatcg ggaataacct gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct
     1381 tccggtgacc gggttcaggt ggtggccgca ctgatggcca tcggggatga gctgcgccgg
     1441 ctgcgtctgg ccgtcaggga acaggggacg cgggatgata gttaagtttc atgccagggg
     1501 aaaaggcggt ggcagtggtc cggttgatta cctgctggga agggagcgta accgggaagg
     1561 ggcaacggtg cttcagggta atccggaaga agtccgggaa ctcatcgatg ccacgccatt
     1621 tgcgaagaaa tacacgtccg gtgttctctc gttcgcggag aaggagctgc cgccgggagg
     1681 gcgtgaaaaa gtgatggcga gctttgagcg tgtactgatg cccggtctcg aaaaaaatca
     1741 gtacagcatt ctgtgggtgg agcaccagga caaggggcgg cttgagctga attttgtcat
     1801 tccgaacatg gagctacaga ccggaaaacg cctccagccg tactacgacc gcgcagacag
     1861 gccgagaatt gatgcctggc agacgctggt gaatcaccat tacgggctgc atgacccgaa
     1921 cgccccggag aaccgccgga cgctgactct ccctgataac ctgccggaaa cgaaacaggc
     1981 gcttgctgag agcattacac ggtggataga cgctctttat catgtcgggg agataaaagg
     2041 ccgtcaggat gtgattcagg cgctcacaga ggcggggctt gaagtggtca gggtgacgcg
     2101 aagcagtatc agcattgcag acccgaacgg cgggaagaat atcagactga aaggagcatt
     2161 ctatgagcaa tcttttacag acgggcgcgg agttcgagaa aaagctgaaa gagagagccg
     2221 aatctacaga gaaaatgctg agcgacgagt tcaggaagct cgaagaatct gtaagcaagg
     2281 ctgtgacatc aaacgagacg aaaatcaaag acgctatagc cctgttcaca gcttcgacag
     2341 aggaatcact gaaaaaacat cgggaagggg tgaaagaggc gatgatgcag cacaggaagg
     2401 acgtgttaaa gctggcaggg aatacgggca tgatgttact ggggatggtc attttcctgt
     2461 ttaccgtgag tggcgggacg ctctggtatc ttggcgggat gatacaggcg aacctggaag
     2521 aaatcaggaa gcagaacgag agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt
     2581 atcacgaggg gagcaacgga cgttttctgg tgttgcccga ggggatgaaa gccgaaacga
     2641 actggacgat ggaaaacggg aaacagaatg ccgtaaggct ggtacaggag tgacagagca
     2701 tgacggagct ggaaagccat ttgctgaacg cattagagca gctgcaacag gactatatgc
     2761 agcggctgaa cgaatgggag agcgccttcg tggaattgca gaaaatgttt tcgcttacgc
     2821 aacgggacaa cgcgatgctg aacgagcggg tcatgcagtt gagtcagcag gtgctgcact
     2881 tgagcgagca gacagaacgc ttgagccagt tatacagcga gaactggaga taagagagga
     2941 acggttgata caggagcgcg aacatgcgtt attcctggaa cgggaacgcc agccggaaat
     3001 acaggaacgg acactggatg gcccttcgct gggatggtaa tcagagacag gctaaaatgt
     3061 tgtcagcgcg tttccggtgc attttttggg ataatggttg gttcgggggc accgggaacg
     3121 cgcttcagtc agaccttgct gaaaacacag gaacgagtta aaacgcatac gggcgcgatt
     3181 ctggtgtgtc ttttgtcgct gtgttttgcc tctattgcca gatcgcgctc attcccctga
     3241 acttctgttt tcccgctcag gtccccgtct tcttcgcttc cgaaagcatg aataaaatcc
     3301 gtttcagctg tctctggctg gcaattcctg cgtgattcac atggctgcat agctatgcat
     3361 gtggttaaaa tttaccgggt gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt
     3421 acctgtctgc tgccgtgatc gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt
     3481 acgttaaatt gtgagggaat ggtccccata tactgacttt atcataggga tagggttggt
     3541 agtgggtcga ttgggtttcg gcattgtttc cgttaatgcc aattgcattt ttgtatttga
     3601 tgcaacagaa ggcgtgagat tctcctgggc ctggaaaggc taagtgttta gaattgatgg
     3661 caaaatatct tccgtccgat ttttgaagta gttgttgttt ggctgtctcc caaagggtgc
     3721 tgttacaaac ttcttgaatt tgtagttttt gcaggactct tattactgcg ttgtcatgtt
     3781 ccaattcttt aggggagtgt attattctct cttttaataa gctgtttacc acagggttta
     3841 gttgttgtct aaagcacgca gataatgcca ttgctacaca attttgttcc cgaggaagca
     3901 tgtttggcgg ggtatgccgt gtttgatgtg atatgtctat tctcatagaa ggtctctggc
     3961 ttatagcatc aacaaatacg gctataaaac aaaattttca tagagtgatt caagatcttc
     4021 ttgagatcct tttttgcggc gcgtaatctg ctgcctgcaa ac
//
LOCUS       DAJTSA010000201         4030 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000201 DAJTSA010000000
VERSION     DAJTSA010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.202"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1251)
                     /locus_tag="OO369_004090"
     CDS             complement(<1..1251)
                     /locus_tag="OO369_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5984137.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVM"
     gene            complement(1336..1671)
                     /locus_tag="OO369_004091"
     CDS             complement(1336..1671)
                     /locus_tag="OO369_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR5984138.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(1671..2027)
                     /locus_tag="OO369_004092"
     CDS             complement(1671..2027)
                     /locus_tag="OO369_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR5984139.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2027..3523)
                     /locus_tag="OO369_004093"
     CDS             complement(2027..3523)
                     /locus_tag="OO369_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR5984140.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(3520..3681)
                     /locus_tag="OO369_004094"
     CDS             complement(3520..3681)
                     /locus_tag="OO369_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR5984141.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(3691..>4030)
                     /locus_tag="OO369_004095"
     CDS             complement(3691..>4030)
                     /locus_tag="OO369_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984142.1"
                     /translation="ERDEKGQWAAYDAVHDVRRELWKALLGWMPDPQGGEIVYVGGTL
                     LDLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGPDGDIE
                     HHLEMRFPQN"
BASE COUNT          905 a         1226 c          921 g          978 t
ORIGIN      
        1 catcacgcta cccatgattt ttgttatgcg tgtcaatgtg ccaatcgcca cgcccatcgt
       61 cagtaatgcc gcaccagttt tcgcgatggt ttttacgacc tcaggatttt ctctgacaaa
      121 cgtgcgcact tcttccagaa aaggcttcat ctcttttatg ccttcattga gtgaaggcag
      181 gaaggtttcc cccagcgtgg aagaaatcgc attgatctgg ttctgcagca gcaacaactg
      241 attttctgtc gtcgcggcac gggcggcata ttctttctgc attgagccgc catactgctg
      301 cgcatccgcc acacgattaa agttggttcg caacaaatcc agattcgtga gcagcggcgc
      361 aatggccccc agggattcct tcccgaacag cgctttcagc acagcagcct gtttttcttt
      421 cggcactttt gccagagaat ccagtacgtg cagcatggcc ccgcgggcgt ctttctgcat
      481 atccgccgcc agttttttcg ggctaatgcg caacgagcgg agcacttttt tctgcgattt
      541 ggtggcagaa tccctcgctg tcagcgacaa cataaaattc tttatccccg tcgcagcaat
      601 ttctgactcc acccccattc cggcaatggt tgcccccatt gcggcaatct ctccggaggc
      661 cacacccgca acactgccta aagggccaat acgggtcaca acatcagaaa tctttttcgc
      721 acttgcagga ccggtattgc caagatagtt aatcttatcc gcaagccctg ccacctctcc
      781 ctgtgtcagt ttaaaggcag tgcgccactg cgccatcatc tgaccggatt cttctgccgt
      841 ggtgtcaaag gccacgccca tcttcacggc atcgtcagta aactgcatca gctcgtcacg
      901 ggcgatacca gcctgaccac ccgccgccac aatttcggcg ataccttccg ccgacatggg
      961 cagttctgtt gacaggtcgc gcacctgctc cgtcattgcc ttaaacgctt ccggcgtatc
     1021 cagaccatcc accactttcc ggacatccgc catttttgat tcaagggcaa tggcagattt
     1081 gaccgggagc gccagcgccc ccagtactgc ggttccggca cccgctgcgc ccagtgacag
     1141 gctggcaaac tcctttttaa aacctttcag ctggcgctgc attcccttca gcggtgccga
     1201 taccttatcc acggcagtga tgatcgcctt cagctgaaaa ctgtcagcca tgcttcatct
     1261 cctcgttaat gcggacggcc tcggcttcca gttctgtaaa ctgcgaaaca gccacccgtc
     1321 gcagctccag tggattcagt ttccagaacc aggcaacatt gtagagtcgt ttccggaggt
     1381 gcttcccgtc tccgactggg taaaaaaacg caggatctgc atgctggtct taaaaatatc
     1441 cagttttgcc atctgcgccg ccgatgaaca cgggatcccc gccagcaacg ggatatattt
     1501 cagtgccacc tggctgtcca gtttaatact gccttcaccg gaaataatga aaggaaaacc
     1561 cagcgcctcg atttcgtcat acgtgggctc gcgcaactcc agcacatgca gcatttcgtt
     1621 atgcgctgtc accggttttt ttaaaactat ttctgtaacg ttcattgcca ttactgatac
     1681 cctccctctt cgccgtggaa ttcaagatct gccgtgcctt cttcggcatt atgattagcc
     1741 tctccgtgca gccatgccgc cgaaagcaca tacaccatgc cgtttgccag ctcagcggta
     1801 atcgtcatct ggtctgaggt ggtaattttg tcgaccggaa agtttttggg cactttaaac
     1861 gtgcccttaa cgtaaggtga ccgccaggtc tccttgtaat ccacatcccc tgccatgccg
     1921 acgatgtcat cccggacatt ggtgttcatc ggcacttcaa tgccacctgt cagcgataac
     1981 tgctgaccat ccactttaaa aaaacaggtt ccggcgatct ttgccattat gctgactcct
     2041 ctgaatactg aagacggaac tggttaacca ccgcaaagac acgtaactga ttaacataat
     2101 ccggcgggaa cagcgtattc agtcggttcg ggttatctgc atcacgctca actatcagat
     2161 actgtttaaa cagatcgtaa ttttccacaa taccggcacg ctccatctga cgatatgtcg
     2221 ccagaagttc ccctttgata acggcaggag tgacaatcgc ctgccccgga ccaaaacggg
     2281 taccatcatt tgccagcttg tgacgtccgt acttgcttgt gatgaccgat ttcagtttgc
     2341 gaagaacata tgcactggta tgcagagttt cactgtccag atagctgttg tctgccacgc
     2401 catacgcatt ctttttgtag gtggttacgg aacgctggat ctgcaacgtg ccgccttcca
     2461 cataagccgt cgccacgccg tgagataaaa gggtctgctg ctctgtcatg atgaatcgct
     2521 tacctttcgg tgccggaagc atccccacca gctcccccgt ctgtgtcgga cgggcaggat
     2581 cattccggat aaataccgct tcacgggcaa ggcgactggc aaccagttca tcgacaggcg
     2641 actgggtttc tttttcgtaa ccggcaagcg tgatatgttg ctgattatgc atatctccgg
     2701 caccaaccag ctctgacagc gttcccagtt ttgcggtata gacatgcccg tataactggc
     2761 gcgcataact ccagcgaccg ctgctgtcat tcatttcggt catcatcata ttgatggagg
     2821 cggcatcgtt gaacggcaga ccgataaagt cgaatgcctc atcgcccata gcagcaacag
     2881 cggcggtaag atcaggcgct ccgctaccgg cagcaccgac ttccgtcacc acccgaagcc
     2941 ctgctggcaa aatctcacca ccaccagaac cataataatt cagacagacc ggtaactcat
     3001 taccatacag ccccttatga cgggcagtca gcgtcaccac gcctgcatca gatgacgccg
     3061 taaacggcag ggtgataacc ccgtttaccg cttccttaat cgcggtggca accgcagtgg
     3121 catcatcgcc attcaccaca ggcacctgta cacgggagcg cccgacatac agactcaggg
     3181 tgccgctttc ctctgcttct ccggcaaccg tcaccctgac cgtcgccgcc gcccctctgg
     3241 cttccggtac tgcaataaca tacagttcac cgaaaggatc tgtctgacgg tagacttcca
     3301 ccatacgcgc cagctggctc cccgccccac aaatctggcg ggcataatct gccgatggca
     3361 tcagcaccag gctgttaacc tcaatggctg catcgttgct ggcatgcccg atcagtaatg
     3421 caggcgcgct ggtcaccgct gtatttgccg cagaattgtt catctcggcg tagaacaacg
     3481 gcaccagcgt attcgacgga acagtattaa aacttatcgt catgattttt tagccttatc
     3541 ctgtttaaca cgtaccacat cacccgccgc tatacggcgg aaccagtagc tgctttcttc
     3601 cacatttcgc ccttcggaag gcaaaaggtc tccacgggca gggtcaggaa ccgaccgccc
     3661 tttcaggggt ttcacaaaca tgagggactc ttaattctga gggaaacgca tttccagatg
     3721 gtgctcaatg tcaccatccg ggccagtacc aggatcgatg taatccacat caatactcag
     3781 cagggataaa tccggcaggg cattcacgtc ctctgcctgt cgcgtgtctt cttccgtgat
     3841 ttcatacttc gccgtaaaat caaactggta atacagttcg taacggttca gatccagcag
     3901 ggtgccaccg acataaacaa tctcgccccc ttgcgggtcc ggcatccatc caagcagggc
     3961 tttccagagt tctctccgga catcatgaac ggcgtcatag gctgcccact gccctttttc
     4021 atcacgctca
//
LOCUS       DAJTSA010000202         3806 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000202 DAJTSA010000000
VERSION     DAJTSA010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.203"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            437..2089
                     /locus_tag="OO369_004096"
     CDS             437..2089
                     /locus_tag="OO369_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5984143.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2100..2270)
                     /locus_tag="OO369_004097"
                     /pseudo
     CDS             complement(2100..2270)
                     /locus_tag="OO369_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2403..2522)
                     /locus_tag="OO369_004098"
                     /pseudo
     CDS             complement(2403..2522)
                     /locus_tag="OO369_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2596..3526)
                     /locus_tag="OO369_004099"
                     /pseudo
     CDS             complement(2596..3526)
                     /locus_tag="OO369_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1052 a          877 c          874 g         1003 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggaacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tctaattggt tatgatgcaa tggccgtcgg
      781 taatcatgaa tttgataatc ctctaaatat attacgtcag caggaaaaat gggccacatt
      841 tcctttcctt tctgctaaca tatatcaaaa aagtacaggg aggcgtttat tcagtccatg
      901 gaaaattttc atccgtcaga atttgaaaat tgcagtcatt ggcttgacga cagatgatac
      961 tgcgaaaaca ggaaattctg aatattttac tgatattgag ttccgtcaac ctgctgctga
     1021 ggcgaggagt gtcattgatg aacttaatca gcaggaaaag ccagatatta ttattgcggc
     1081 aacacatatg ggtcactatg acaatggcga gtcaggttcg aatgctcctg gtgatgttga
     1141 aatggctcgc agtttgccga ctggctcatt agcaatgatt gtgggaggac attcacaagc
     1201 tcctgtctgc atggccagcg ataataaaaa acagtggaat tatattccgg ggacaacctg
     1261 tgtaccagat aaacaaaatg gcatctggat tgtacaagcg catgagtggg gaaaatatgt
     1321 cggtcaggct gattttgaat tctgtaacgg tacaatgaaa ctggttaatt atcagttaca
     1381 cccagttaat ctcaaaatga gaattaccag agaagatgga aaaacggaat tttcttttta
     1441 cacccccgaa attactgaag atccacagat gttatcctta ctgacaccat tccagaataa
     1501 aggcaaagca caactggatg tgaaggttgg ggttgtgaac ggacgtctgg aaggggacag
     1561 aagtaaagtt cgttttgttc agaccagtat gggccatctg attctgagcg ctctgacaga
     1621 acgtattgac gcagattttg ctgttgtcag tggtggggaa attcgagatt ccatagagag
     1681 tggtaacatc acatacaaag atatactgaa agttcagcca tttggtaata cggtagtaag
     1741 tattgatttg acggggaaag aagtagctga ctacctagct acagtggcac agatgaaacc
     1801 ggattccgga gcctacccac aatttttgaa taccagcttt gtagtcaaaa aaggaaaaat
     1861 cgaaatgcta aaaataaaag gtaaatcggt tgaccttaac aaaaaatatc gtatgacaac
     1921 atttagcttt aatgctactg gtggggatgg ctatccacgt attgataata gacccggata
     1981 cataaatacc ggatttattg atgctgaagt acttatcgaa tatatccgga aacattcacc
     2041 gctggatgct gcttcatatg aaccaaaagg tgaagtcagt tggcagtgat aacgtaattg
     2101 acgatcatca cgtgtttttt gctgacagtc gctgtgactt tgtgaaagta gtctctgcgt
     2161 atgtttgcga tatgggaatg cagttgctgt attttgtgtt tctgcttctg ccagttgttg
     2221 ctgaatttaa ccttgcggct taactgatgt tggagcgctg ccagcttctt cgtcaatttc
     2281 gctgattaaa gtttattcat tatggcatga tctgcgtaag cggctcgtca gaaccatatt
     2341 gatatttact aagagctcag atcaactttc cagggcaaca gatcgcgtac ccggtttgcc
     2401 ggtcagtcct ggatatgctc aatgacgtaa cgcagccatt tttctggctc cacattgttc
     2461 agacggcatg tgccgatcag cgagtacaac accgccgcat gttcgccacc gctatcggaa
     2521 ccccgccgga gagagtttca ctctccggct gctcacggaa ccgtacgtga cactctcgcg
     2581 tcatacggct cttgatcact cattcgatgc acgtagtaaa taccagtggg caaatagctt
     2641 cggatactgt ttccttacca gcgccagctt tcgctgagcc ttccggttcg aaagtctgta
     2701 tttagactgc atccacttaa gcaaacgtga ctgcattgca ctccacagtc gatagctgaa
     2761 gtttctggac cagaactttc catagtactc aatccagcct ctcactatcg cattgtagcg
     2821 cctcgcaaaa tccagcaaac tctcagctgt tgagcgatgt atacgccact tcttgattgt
     2881 ttctgttatt ttgcacattg ctgcatttga tgcacccggc atacattttc tgtacaattc
     2941 gcctttgaaa ttcttcagag tacgcacttt gaagtcatat ccgagagtgg catacaacat
     3001 catcggcgta cctttcgaag ggaacgccgg gaaactgacg ttgcatccac atatcaaagg
     3061 catagtgcag gaacagattt gagagcaggg gggagattac gccgccctgc ggcgttcctc
     3121 gaccccgtgc cagcattgct ccatccggca tttgcaccgg tgccttcagc ctgcgctcaa
     3181 tgtacatcac cacccagcgt gcctgagtat gatgctgcac tgtcttcagt agcaggtcat
     3241 gatcgatatc atcaacgaag cctttaatat cgacttccac cacccagtta aacttccagc
     3301 agcgtttcct cgcctgcgca atcgcctggt gtgcggattt acccggtcga taaccatacg
     3361 agtctgcatg gaacaggggt tccagtaccg gctcgagtat ctgcttaacg accatctggg
     3421 cgatccggtc cgacacggcc ggaataccga gcggacgtac accaccgtca gctttgggaa
     3481 tgtccacacg ccttaccgcc tgcggcatat aactgccgga agccattctg ttccacagtt
     3541 tgtagagatt gtctgtaaca ttctcgtcaa acccggccag tgtctgcccg tcaatgcctg
     3601 ccgccccacg gttggctttt actcgtttat aagcttccca tacctgtcgt ttacttacag
     3661 gaaatggctt tgccgatttt acagcagtcc tcccatcggc attgtcgccc gtggttgccg
     3721 ccgcaccatc agcacaatgg ggcaagccct ttgctccgga cccattacgg tccttcaccg
     3781 ctactacggt ttactccgtc cctgta
//
LOCUS       DAJTSA010000203         3687 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000203 DAJTSA010000000
VERSION     DAJTSA010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.204"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..221
                     /locus_tag="OO369_004100"
     CDS             <1..221
                     /locus_tag="OO369_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5984144.1"
                     /translation="QRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLPV
                     LQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            267..476
                     /gene="hha"
                     /locus_tag="OO369_004101"
     CDS             267..476
                     /gene="hha"
                     /locus_tag="OO369_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5984145.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            515..1105
                     /locus_tag="OO369_004102"
     CDS             515..1105
                     /locus_tag="OO369_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR5984146.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1345..1605
                     /gene="repA"
                     /locus_tag="OO369_004103"
     CDS             1345..1605
                     /gene="repA"
                     /locus_tag="OO369_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR5984147.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1829..1903
                     /gene="tap"
                     /locus_tag="OO369_004104"
     CDS             1829..1903
                     /gene="tap"
                     /locus_tag="OO369_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR5984148.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1896..2753
                     /gene="repA"
                     /locus_tag="OO369_004105"
     CDS             1896..2753
                     /gene="repA"
                     /locus_tag="OO369_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR5984149.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3454..3645
                     /locus_tag="OO369_004106"
     CDS             3454..3645
                     /locus_tag="OO369_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984150.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT          997 a          901 c          928 g          861 t
ORIGIN      
        1 ttcagcgaga tcgttatggt cgtattctcg ggcaggttta tgcaccagac ggaatgaata
       61 ttaatcagtt tatggtccgg gccggggctg cctgggtata tgagcagtac aacacagatc
      121 ccgtgctacc tgtccttcag aacgaagcca gacaacaaaa acgtgggctc tggtcagatg
      181 ctgatccggt tcccccctgg atatggaggc accgtaaata aagcgatggc aatcctatca
      241 ttcatctgtt ctcagcggga ataaaaatgg caaaaacaaa acaggaatgg ctgtatcagt
      301 tacggcgatg ttcatcagtc aatacgctgg aaaaaattat tcataaaaac agagacagtt
      361 tatcaaacag tgaacgtgaa tcatttaatt cagcagcaga ccaccggcta gcggaattaa
      421 tcaccggaaa gttatatgac aggataccaa aagagatatg gaaatatgta aggtaataat
      481 tcagcaataa ttaattgcca ggagcagata aataatgaag ttgattattt ttattctcat
      541 tgttctgatt attgcagcgc tacttatccg gattatcctc agaagtgtaa atcagcattc
      601 tcctttgctg atgcaattgc atgcggcagg tatacggact ggtgatgcag agcgaatact
      661 gtccagcggt gaatactggc aacgtcagaa gacgctgctg acagaaaggg aagtcagttt
      721 tatgaaagga ctgttcagaa ttgtggatat gaagcggtgg tatctgtgtc cgcaggtacg
      781 ggtagcggat atcgtccagc tgaacgggaa tatccggcca cgatcacgcc agtggtggca
      841 gttattcagg atggtgtctc agtggcatgt tgatgtggtt atcgttgagc tgcgttcgtt
      901 cagtattgtt gcagcagtag agctggatga tgccagccat ttacgaccgg aacgcagacg
      961 ccgggatatt cttctggaag aggttctgag gcaggctggt attccgttgc tcagaagcca
     1021 cgatgccaga aaactgctgc agatgaccgg agaatggctg aatacaacag gggctgatca
     1081 gcagtcaccg gaacatcgta gctgacgcct tcgcgttgct cagttgtcca accccggaaa
     1141 cgggaaaaag caagttttcc tcgctcccgg cgtttccata actgaaaacc atactatttc
     1201 acagtttaaa tcacattaaa cgacagtaat ccccgttgat ttgtgcgcca acacagatct
     1261 tcgccacaat tctcaagtcg ctgatttcaa aaaactgtag tatcctctgc gaaacgatcc
     1321 ctgtttgagt attgaggagg cgagatgtcg cagacagaaa atgcagtgac ttcctcattg
     1381 agtcaaaagc ggtttgtgcg cagaggtaag cctatgactg actctgagaa acaaatggcc
     1441 gctgttgcaa gaaaacgtct tacacacaaa gagataaaag tttttgtcaa aaatcctctg
     1501 aaagatctca tggttgagta ctgcgagaga gaggggataa cacaggctca gttcgttgag
     1561 aaaatcatca aagatgaact gcagagactg gatatactaa agtaaagact ttactttgtg
     1621 gcgtagcatg ctagattact gatcgtttaa ggaattttat ggctggccac gccataaggt
     1681 ggcagggaac tggttctgat gtggatgtac aggagccaga aaagcaaaaa ccccgataat
     1741 cttctttaac tttggcgagt cagaaagatt accggggccc acttaaaccg tatagccaac
     1801 aatcaagcta tgcggggagt atagttatat gcccggaaaa gttcaagact tctttctgtg
     1861 ctcgctcctt ctgcgcattg taagtgcagg atggtgtgac tgatcttcaa caaacttatt
     1921 accgccaggt aaagaacccg aatccggtgt tcacaccccg tgaaggtgcc cggacgcttc
     1981 cgttctgcgg gaaactgatg gaaaaggcgg tgggcttcac ctcccgtttt gatttcgcca
     2041 ttcatgtggc acatgcccgt tcgcttggtc tgcgtcggcg catgccaccg gtactacgtc
     2101 gccgggccat tgatgcgctg ctgcaggggc tgtgttttca ctatgaccca ctggccaacc
     2161 gcgtccagtg ttccatcacc acgctggcca ttgagtgcgg actggcgacg gagtctgctg
     2221 ccggaaaact ctccatcacc cgggccaccc gggccctgac gttccttgca gagctgggac
     2281 tgattaccta ccagacggaa tatgacccgc ttatcgggtg ctacattccg accgatatca
     2341 cgttcacatc tgcactgttt gctgccctcg atgtatcaga ggaggcagtg gccgccgcgc
     2401 gccgcagtcg tgtggaatgg gaaaacagac agcgcaaaaa gcaggggctg gataccctgg
     2461 gtatggatga actgatggcg aaagcctggc gttttgttcg tgagcgtttc cgcagttacc
     2521 agacagagct taagtcccgt ggaatgaagc gtgcccgtgc gcgtcgtgat gcggacaggc
     2581 agcgtcagga tattgtcacc ctggtgaaac ggcaactgac gcgtgaaatc tcggaagggc
     2641 gcttcactgc cagtcgtgag gcggtaaaac gtgaagtgga gcgtcgtgtg aaggagcgca
     2701 tgattctgtc acgtaaccgt aattacagcc ggctggccac agcttccccc tgaaagtgac
     2761 ctcctcagaa taatccggca cgcgccggag gcattcgcac gcctgaagcc cgccagtgca
     2821 caaaaaatca gcgctacata caaaaaacaa cctcatcatc caccttcagg tgcatccggt
     2881 cccccctgtt tttgatacaa aacacgcctc acagacgggg aattttgctt atccacatta
     2941 aactgcaagg gacttcacca taaggttaca accgttcacg tcataaagcg ccagacgtca
     3001 accctacagg gtgcaatgta tcttttaaac acctgtttat atctccttta aactacttaa
     3061 ttacattcat ttaaaaagaa aacctgttca ctgcctgtcc tgtggacaga caaatatgca
     3121 cctcccaccg caagcggcgg gcccccaacc gaagccggtt tagttacaac attcaaacat
     3181 aaacccctaa aaaacccaag tccaacgcaa aactgaaacc acaacgcccc cctcataact
     3241 gaaaagcgcc gccgcccccg cccaaagggc gggaacagag tcgcttttaa ttatgaatgt
     3301 tgtaactaca tcatcatcgc tgtcagtctt ctggctggac gtatcgaata cacgctcgta
     3361 agcggccctc acggcccgct aacgcggaga tacgccccga cttcgggtaa aaccctcgtc
     3421 gggaccactc cgaccgcgca cagaagctct atcatggctg aaaacgggta tggcttggca
     3481 gggctgggga tgggtaaggt gaaatctgtc aatcagtacc ggctgacgcc gggcttcggc
     3541 ggttttactc cggtatcaca tgtaaccaca gcgtgccgcc ttccatgccg ctggcgcggc
     3601 atcagaataa tacaagcagc atttaatgca tttgcaaaag tgtgagtaat attaagtggt
     3661 cgtttcaggg atatcgttaa gacttga
//
LOCUS       DAJTSA010000204         3548 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000204 DAJTSA010000000
VERSION     DAJTSA010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.205"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="OO369_004107"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="OO369_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR5984151.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="OO369_004108"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="OO369_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR5984152.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="OO369_004109"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="OO369_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR5984153.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          773 c          819 g          950 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tctataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctggc
//
LOCUS       DAJTSA010000205         3457 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000205 DAJTSA010000000
VERSION     DAJTSA010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.206"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..689)
                     /locus_tag="OO369_004110"
                     /pseudo
     CDS             complement(1..689)
                     /locus_tag="OO369_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(696..1473)
                     /locus_tag="OO369_004111"
                     /pseudo
     CDS             complement(696..1473)
                     /locus_tag="OO369_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1551..1973)
                     /locus_tag="OO369_004112"
     CDS             complement(1551..1973)
                     /locus_tag="OO369_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984154.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            complement(1957..2184)
                     /locus_tag="OO369_004113"
     CDS             complement(1957..2184)
                     /locus_tag="OO369_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5984155.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            2261..2668
                     /locus_tag="OO369_004114"
     CDS             2261..2668
                     /locus_tag="OO369_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5984156.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            2872..3027
                     /locus_tag="OO369_004115"
     CDS             2872..3027
                     /locus_tag="OO369_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR5984157.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            3029..3232
                     /locus_tag="OO369_004116"
     CDS             3029..3232
                     /locus_tag="OO369_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984158.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
BASE COUNT          868 a          939 c          764 g          886 t
ORIGIN      
        1 acccagcgcc cgccgttcat gaccatgcga tccatcaccc gctcgccgag caatgttttc
       61 atcacctcat agttcaggtt tgtcagcatc cccacgctgc gcatcgacgc tgtccggcga
      121 tcaacaatct ggtgcagcac cacctgctcg tttttcgtct cgcgctgaat gccaatttca
      181 tcaagaacca gcagatccac ttcgcacagc tcccgcaaaa atttttcgcc tgactgcccg
      241 tcgtcatagc tggcgtgcag ggcgctcata acatcagcca cggtaaccac aatcactgtc
      301 tgaccgtctt tcagcaggcg attcccgata gctgccgcta agtgattcta cccggtatca
      361 ggttttccgc tgaacgcaaa atttgtacac ccggtcatca gttcatcggc gatggatttc
      421 gcctggctta acgcgtatcg ctggccgtcg ttctgcacct ggtaattcgc aaacgagcat
      481 ttacggtgca acggctggat gcctgagcga ttcagaattt tttccacccg caactggcga
      541 ttcaggcggt tgatctcctc gctacgtttc tggccttcag caagttgcca ctcgcgccac
      601 tccgctaccg ttctgaatgg ggcggttaca tgttgcgggg tcagtcggcg gatgcgttcc
      661 agaacgccgc ctgtcgcaat atttttcatg gtccgttacc ccctgaagcc tggcgggatc
      721 gcactgtccg gcaacgagac ggtgttaacc tgtcggagca acgtctcagg ccgaacacct
      781 ttcggcgcga acaggccctg gtattcattg gcgatgctgt gtcgaatcac ctgctcaggt
      841 gtaaaaccct gctgacggaa tttttccagt tcccgtatcg ccctgctccg ttcgaatcgg
      901 ttttcgcaat gcctgtctga accggaccca ctcatgccag agtgtttccg gcaaccaatc
      961 gggcagctca atagcctccg gctcgaattt tttagacgct cgtttttggc gagggggatt
     1021 tagggggaga tcagtattta gatcttcctc ttcctcttcc actggtaacg ctttttgatc
     1081 cgtttgtgta acgctggcag cgttaccttt tcgtttcagt tcgcgtattt ttgttactcg
     1141 ctcgtttgta accgcccgtt ttttagagct ttttccgtta tgacgttcaa agttaggtag
     1201 agaaagccca ccgtcatttt cgaccagcca tccaacctga attaacgcat cagcaaaacc
     1261 agacataaaa gtgatgcgat ctattgcact ttttgtaacg ccgcgagcgt tacaatctgc
     1321 attaccgtct atcatttgtt gatccgccca tgcccagaag cgaataacct tccctaatgc
     1381 ggcatctgga tcaatattca gaatctcagc aagcctgaat atttccggct tatccggcgt
     1441 aataacctcg agctttatcc agtttgaagc catttgtttt caccttgtaa cgctcgcagc
     1501 gttacattta actgataccg aacaaaacaa tccggcacga ttaatttcaa tcaatgcact
     1561 acgacagaat cgccgggcga cccaccaccg ctgaaatgtg ctttccggta aacggcctgg
     1621 acagcatcat catgcgcatc aattgccgta ctcaacgctt cctgcgccgc cagtaatgca
     1681 cggcgttcca gggtatcgaa gatacagagt cggtgacgca gctcacgcgg aagaattgcc
     1741 agaaccgcag ggatcagttt ctgaattttt tccctttgcg ctttcgtttc acctttcaac
     1801 caacggtgat agatattctg ctgattgttc cagtccttgc ctggtaccag agacaattcg
     1861 ccgcccccct gccgcagata ttcttcagta attgcgttag cgacccacgc ctgccctttt
     1921 tcggctgcca gggctaacag cactgattcg atgtgctcat gcctgatttt catgaatcac
     1981 cttcccaggt cgttttgcct tacgatattc gtcataaact ttggggtcgt actgaagttc
     2041 cccgccagat gcctcttgca ggcgcatcgc gcgaccttca ggaaccagtt ccccccatgc
     2101 agcaatgctt gccagcctaa ctcctgcggc attggcaagc tttgttttgc tgccaaaaaa
     2161 cgctatagca tcaattttca acatatcgag ctccttagat tttcctaagg aaaccaggcc
     2221 gtagagaaac ctaagtcaag aaaaattaga attccctaat atgaaaaacg aaaccttcgg
     2281 tgctcgcctc ttacataggc gtaaaaaatt aaaactgtct caggccgcat taggtaagct
     2341 ggtcaaagtg gctcacgtaa caatttctca atgggaaaga gatgaaacac agccggcggg
     2401 gaaaagatta ttcgcactga gccaagcgct tcagtgctca ccgacttggc ttctttttgg
     2461 ggatgaagat aaacaaccag gcgaaccgat cccggataat cagccagcca ttctgacaga
     2521 agatcaaaaa gagttacttc aactgttcga cgcactgcct gagtcagagc aaaaggccct
     2581 gttgtcagag atgcgtgctc gagttgaaaa tttcaacaaa ctttttgaag aactactcaa
     2641 agctcgcaaa agaagcgcaa acaaataatc cccttttttc tccacaccct gtaataaaaa
     2701 gcacaaactt tcaaacactt gtgtttttta catcaaaaaa acttaggttt ttctacacaa
     2761 aaatcttgat cactctcctt aggttattct aaattttatt catcaagaca ccgcacggtg
     2821 ttctcagcaa acagttccgc taccccggcg ttaaggggaa atggggtcaa catgaatact
     2881 atcgatcttg gcaacaacga atctccggtg tacggcgtgt tccccaacca ggacggcaca
     2941 ttcaccgcca taacgtatac caaaagcaaa acgtttaaaa ccgaagctgg cgcacgtcgt
     3001 tggttgggaa gacattcagg tgagtaaaat gaacgagaca gaattaaaac acgttatcgc
     3061 tctactccta gaagatgcaa aacgcctcca gcaactggag ccaaatgcag gcactggggc
     3121 acgcatctgg ctggctaaag aagcgctgga atctggcgat tatgatagcg aagaagcctt
     3181 ctacaaagca gaaggccgtg caggatattc accgggtctt ggcggggtat aaataccatg
     3241 cgcattgact gaattcgcaa acaaaaacag acgcgcgata cccggatagt cggtctgcat
     3301 agtcagatat ctggctgcga atttgataaa tatcaccgcc cttttcggaa acataagttc
     3361 cgtccgggag ttgattatac gaaccatctc catgggggat cgttctcctg aatcctagtg
     3421 agagcatata tttatgaagc ccttcgtaat cctctgg
//
LOCUS       DAJTSA010000206         3413 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000206 DAJTSA010000000
VERSION     DAJTSA010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.207"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..237
                     /locus_tag="OO369_004117"
     CDS             <1..237
                     /locus_tag="OO369_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCR5984159.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            247..885
                     /locus_tag="OO369_004118"
     CDS             247..885
                     /locus_tag="OO369_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR5984160.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGNGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            888..2051
                     /locus_tag="OO369_004119"
     CDS             888..2051
                     /locus_tag="OO369_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5984161.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2135..>3413
                     /locus_tag="OO369_004120"
     CDS             2135..>3413
                     /locus_tag="OO369_004120"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5984162.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEG"
BASE COUNT          760 a          803 c          986 g          864 t
ORIGIN      
        1 ttgttaggtt acgttatccc attatccagt gcggcaatca atgcggtgag tattccagac
       61 tatttcgcct ggggcgggat tgcactggtg attcagttac tcgtttttgc tggtgtcaga
      121 ctttatatgc ccgcattaag cgaaaaaatt attaatcaca ataccgcagc aggaatgttt
      181 atgggaaccg ccgcgctggc tggcggtatt tttaacgcag cttgtatgac atggtaatgg
      241 acgatcatgg ccagaaaacg caaatcaaga aataacagta aaattggtca cggagcgatc
      301 agtcgcattg gtagaccgaa taatcctttt gaaccgcgtc gcaatcgcta cgcacaaaaa
      361 tacttaacgt tggcgctaat gggcggtgcc gcttttttcg tattgaaagg ttgtagcgat
      421 agtagcgatg tcgataacga cggcaacgga acattttacg cgacagtgca ggattgtatt
      481 gatgacggta ataatgccga catttgcgcg cgtggctgga acaacgccaa agcggcattt
      541 tatgccgatg ttccaaagaa tatgactcag cagaactgtc agtctaagta cgaaaattgc
      601 tactacgaca atgttgaaca gagttggatc ccggtcgttt ctggattttt gttaagccgg
      661 gttattcgta aagatcgcga tgagccgttc gtctataaca gcggcggatc ctcatttgct
      721 tcccgccccg tctggcgcaa cacttccggt gattactcct ggcgcttcgg ttctggcaaa
      781 aaagagtctt actcttcagg cggctttacc accagaaaag cgtcgaccgt ttcgcgcggc
      841 ggctatggtc gttcttccag cgcccgtggg cattggggag gctaatcatg ctgagacaca
      901 acgttcctgt gcggcgggat ctggaccaga tcgccgctaa caacggtttc gactttcata
      961 tcatcgacaa tgaaatctat tgggacgaga gtcgggctta ccgttttact ctgcgccaga
     1021 ttgaagagca gatcgaaaaa ccgactgcgg aactgcatca gatgtgcctt gaggtggtgg
     1081 atcgcgcggt taaagacgaa gagatcctga cgcaactggc gatcccgccg ttgtactggg
     1141 atgtgatcgc cgaaagctgg cgcgcacgcg atccttcgct gtatggccgg atggattttg
     1201 cctggtgtgg caatgcgccg gtgaagctgc tggagtacaa cgccgatacg ccgacctcgt
     1261 tgtatgagtc ggcttatttc cagtggttgt ggctggagga tgcccggcgc agcggcatta
     1321 ttccgcgtga tgccgatcag tacaacgcca ttcaggaacg cctgatttcg cgctttagtg
     1381 agctttacag tcgggaactg ttttattttt gctgctgtca ggacaccgat gaagacagga
     1441 gtaccgtgct gtacttgcag gactgcgccc agcaggcagg gcaggagtcg cggtttatct
     1501 acattgaaga tctcggtttg ggcgtcggcg gcgtactgac cgatcttgat gataatgtca
     1561 tccagcgtgc atttaagctg tatccgctgg agtggatgat gcgtgacgat aacggtccgc
     1621 tgctgcgcaa gcgtcgcgag caatgggtgg agccgttatg gaaaagtatc ttgagtaata
     1681 aagggctaat gccgctgctt tggcgcttct tccctggtca tcctaacctt cttgcatcct
     1741 ggtttgaggg tgaaaaatcg cagattgccg ctggcgaaag ctatgtgcgt aaaccgatct
     1801 actcgcgcga aggcggaaac gtcaccattt tcgacggtca gaataacgtc gttgaccacg
     1861 ctgatggtga ttacgccgat gaaccgatga tctaccaggc gtttcaacct ctgccgcgat
     1921 ttggcgatag ctacacactc atcggtagct ggattgtcga tgatgaagcg tgcggaatgg
     1981 ggatccgtga agataacaca ctgatcacca aagacacctc acgtttcgtt ccgcattaca
     2041 ttgctggata agaatgtttt agcaatctct ttctgtcatg aatccatggc agtgaccata
     2101 ctaatggtga ctgccattga tggagggaga cacagtgcac tggcaaactc acaccatttt
     2161 taatcaacct ataccattaa ataacagcaa cttatacctg tctgatggcg cgctttgcga
     2221 agcggtaacg cgtgaaggtg ctggctggga tagcgatttt ctagccagta ttggtcagca
     2281 gttaggaacg gctgaatccc ttgaactggg gcggctggcg aatgtaaacc cgcctgaact
     2341 gttgcgctac gatgcccagg ggcggcgtct ggacgatgtt cgtttccatc ccgcatggca
     2401 cctgctgatg caggcgttat gtaccaatcg ggtgcacaat cttgcctggg aagaagacgc
     2461 tcgctccggc gcatttgtgg cgcgcgcggc gcgttttatg ttacatgcgc aggttgaggc
     2521 tgggtcgtta tgtccagtaa cgatgacctt tgccgccacg ccattgttgt tacagatgtt
     2581 acccacgccg tttcaggact ggaccacgcc gctgctgagc gatcgctacg attctcactt
     2641 attgccaggt gggcaaaaac gcggtttgtt gattggcatg ggaatgacgg aaaagcaggg
     2701 cggttccgat gtcatgagca acaccacccg tgcagagcgt ttggaagatg gctcttatcg
     2761 gctggtgggg cataaatggt ttttctcggt gccgcaaagc gatgcgcatc tggtgctggc
     2821 gcagaccgcg ggcggtctgt cctgcttttt tgtgccgcgc tttttgcctg acgggcaacg
     2881 caacgcgatt cgcctcgagc ggctgaaaga taagctgggt aatcgctcta acgccagttg
     2941 cgaagtggag tttcaggatg ccattggctg gttattgggg caggaagggg aaggaattcg
     3001 tctgatcctg aaaatggggg ggatgacgcg ttttgattgc gccctgggta gccatgccat
     3061 gatgcgccgt gcattttcgc tggcgattta tcatgcacat caacgccatg tttttggtaa
     3121 tccattgatc caacagcccc ttatgcgtca tgtcttaagt cgcatggcac ttcagcttga
     3181 agggcaaacg gcgttgctgt ttcgtcttgc gcgagcgtgg gaccggcgtg ccgatgccaa
     3241 agaagccctg tgggcgcgtt tatttacgcc tgcggcaaaa tttgtgatct gcaaacgagg
     3301 tatgccgttt gtggccgaag cgatggaggt gctgggcggc attggttatt gcgaggagag
     3361 cgagctgccg cggctttacc gggagatgcc ggtaaacagt atttgggaag gtt
//
LOCUS       DAJTSA010000207         3357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000207 DAJTSA010000000
VERSION     DAJTSA010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.208"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            19..897
                     /locus_tag="OO369_004121"
     CDS             19..897
                     /locus_tag="OO369_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5984163.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            894..2285
                     /locus_tag="OO369_004122"
     CDS             894..2285
                     /locus_tag="OO369_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5984164.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2282..2539
                     /locus_tag="OO369_004123"
     CDS             2282..2539
                     /locus_tag="OO369_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984165.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2634..2834
                     /locus_tag="OO369_004124"
     CDS             2634..2834
                     /locus_tag="OO369_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCR5984166.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2827..3207
                     /locus_tag="OO369_004125"
     CDS             2827..3207
                     /locus_tag="OO369_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5984167.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
BASE COUNT          886 a          728 c          973 g          770 t
ORIGIN      
        1 cattctgagg tgacagcgat gatgacgttt aacctgcgtg aacaacaaaa aagactacag
       61 gcgcgaatgg atgagttacg ggcagagatt gcatttgctc agaagggcga aaagccatgg
      121 ccttatcgtt cctgcctgat gcgtgaaggt cgcggatatt gcgaaaaaca tggcgaatat
      181 cacacgcata tactggtgtg gagcgatcgt aatggcgagg acagagaaga aatttcatgc
      241 tgccctgact gcttaatcgc tgaggccaac gatttgacca tggagctgtc gtccatcaag
      301 gcggaagagc tgactgataa cgccggaatt gccctgcgtt ttcgggactg cgagtttgat
      361 aattatctgg aggttaatcc tggcgcagcc agaaatcttg cggcctgtcg ccgctatgcg
      421 gagaactggc cagatatgct ggagaacggt accagtcttg ttatgaccgg cagttgtggt
      481 accggaaaaa atcatctggc tgtggctatg gcaaaacaca tcatccgtaa ctatctggcc
      541 agtgtggaga tcaccgacgt gatgcgcctt acccgtgctg tgaaaaattg ctggcggaat
      601 gacagcgaaa aaacagcgga tgaagttatt gagcgttatg cgtcaatgga tttgctgatc
      661 atcgacgaag ttggcgttca gtttggcagc gcggctgaaa tggctatttt gcaggaaatt
      721 atcaacgcca ggtacgaaag tatcttgccc accattctga tcagtaacct ctcaccggaa
      781 gagttgtggg cgttcatcag tccccgaatt gccgacagga tcacagacgg gggacgcaac
      841 tggttgtcgt ttaactggcc cagttaccgt tctcgtatca gaggtgtggc tgcatgacaa
      901 ctccagtatg gcgtaacgat gaccttgaag gcgctgtcat cggcgcgttc tttctgcgcg
      961 gagcagatcc tgaagtgatg gatattctgg ccacactgcc agcggacgtt ttttctgtac
     1021 gagcgtatca ggatatctat acaggcatct gcagacaggc ccgtgtttca ggagtgattg
     1081 accccgtgct gttgtgtaat gagatgccgg aacttgcccc ggtgattact gataccgggc
     1141 gtaaaacctg ggtgaagtct tcactggagc actatgttgc agcgttgcgg cgcaatgccg
     1201 cactgcgcga tgcagaaaaa acactgaatg aggcgctgca gaaattacgt gatgcgcata
     1261 cctgtgaagc agctgaagat gccctgaagg atgcgcagaa catgatggtc acactgtcga
     1321 caggaaaggg cgtcattcag ccggtacata ttgatgatgt gcttccggaa gtggttgagc
     1381 gtgttgaatg ccggaatcag gggctggaga aatccaggac gttgatgacc ggtattgatg
     1441 aactggacgc aaaaacaggc ggtatggagc ccggagacct ggtattcatt gccgcccgtc
     1501 cttcgatggg gaaaaccgaa cttgcgctgg acatcatcga caaggtgact gagcaggggc
     1561 atggtgtgct tctgttcacc atggagatgg cgaacatcca gattggtgaa cgtatggtgt
     1621 ctgcggctgg agggatgcca gtatcacgcc tgaaatctgt ggctcacttt gaagatgaag
     1681 actggacgcg tttctcacaa ggggtggggc ggatgaccgg gcgcaatatc tggatggtgg
     1741 accaggcgaa cctggccatt gatgagatat gcgcaacaac gaaacaccac ctgattaaat
     1801 atccggaaac ggcgctggtg gtggttgatt atctcgggct gataaaaacc cgaaccacgg
     1861 ggcgtcatga ccttgccgtg ggtgaaatct caaaggggct taaaggcctg gcaaaatccg
     1921 gtggttttcc gttgattgcg ctgagccagc tctcccgcgg tgtggagtcc agacccaata
     1981 aacgtcccat gaactcagac ctgaaaaatt ccggagaaat agaggcggat gcagacatca
     2041 ttctgatgct ttacagggat gaagtgtaca acccggatac gcaggccagg ggcatcgcag
     2101 aaatcaatat cacgaaacaa cgtaacggtt ctctgggaac gatttaccgg cgtttttata
     2161 acggacattt tctgcctgtg gaccaggaaa gtgcacgggt gctttccact cccatgaagc
     2221 ccggcaatcc gcgcagatac agtaataaac gaactgacag cagtaagatg gagcgtttct
     2281 tttgaacaac cagacaatga cttttacccc tgaacaattg cgtaaacacg cgcaggaaat
     2341 gctgcgtcat gctgaacaac tcgaaaaaac gggcataaca aaagatgcta tccgtaaaga
     2401 tatggtaccc gcgcttcggg aactgatgca ggcgaagcat cgcgcacaga aagcagtaga
     2461 tgagctggtg gactgtgtgg cagagctgga aaccagagtc ggaaagtttg aaaaactggt
     2521 gcaggaggcg ctgcgctgat gcgccatgag tttgttttgc cctggcttgt gcgcgatcat
     2581 tatgtccctg gtggaaagcc cgggataaaa atcaccgaac cggagagtat ttgatgagcg
     2641 tcaaaattca aacgataccg gaactgctta tccagacacg aggtaatatg accgaagttt
     2701 cacgaatgct gaactgtaat cgcgccacgg taagaaaata tgctgaagat aaagagggca
     2761 aagggcacgc cattgttgat ggtgttctga ttgttcatcg tggatgggac aggggtaaag
     2821 acagtgatgc gtgatattca gcaggttctt gagcgctggg gcgcgtgggt ggctaataat
     2881 catgaggatg tgacctggtc gtccattgct gccggtttta agggattaat cccttcaaaa
     2941 gtaaaatccc gcccgcaatg ctgtgacgat gacgcgatgg ttatatgtgg gtgtatggcc
     3001 cgccttaacc ggaacaacaa cgatctacat gacttgctgg ttgactacta cgtgtttggg
     3061 atgacattca tgacgctggc gcggaagcat ggatgctcag atggttatat aggaaagaag
     3121 ctacaaaaag ccgagggggt ggtggaagga atgttgatga tgttgggtgt ccggttagaa
     3181 atggacaaat atattgaaca tccctgatgg taaggggcga ctttctagaa aatattttac
     3241 gtacgtaaaa aacgggatat tctgttaaga gtggttgctt cgccacacag cttaaacccg
     3301 cctccgggcg ggttttttat gcctgaaaaa cggtacagga cgttaaacac gctggtg
//
LOCUS       DAJTSA010000208         3324 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000208 DAJTSA010000000
VERSION     DAJTSA010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.209"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            454..1281
                     /locus_tag="OO369_004126"
     CDS             454..1281
                     /locus_tag="OO369_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5984168.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1325..2074
                     /locus_tag="OO369_004127"
     CDS             1325..2074
                     /locus_tag="OO369_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR5984169.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            2071..2640
                     /locus_tag="OO369_004128"
     CDS             2071..2640
                     /locus_tag="OO369_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984170.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2764..3006
                     /locus_tag="OO369_004129"
     CDS             2764..3006
                     /locus_tag="OO369_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5984171.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            3010..3156
                     /locus_tag="OO369_004130"
     CDS             3010..3156
                     /locus_tag="OO369_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984172.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            3165..>3324
                     /gene="xisR"
                     /locus_tag="OO369_004131"
     CDS             3165..>3324
                     /gene="xisR"
                     /locus_tag="OO369_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HCR5984173.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPK"
BASE COUNT          928 a          796 c          854 g          746 t
ORIGIN      
        1 acgccatcct gacaagcgtt ttatcttcaa cgggaatgag atatggaaac gtggagaatc
       61 aaccacagag tatacccgcg cacaccagcg aacaatcaga cgactgcgca gactcatcgc
      121 caggaaacag tatacagaaa aatggttcag aaaatatgac acatggagcg ccggatatta
      181 cgaactgatg gcaacaaaac cattctgacg taaacgaaat taaccatgac gcaattaaat
      241 aaggcaagcc gaatacatca ggaggaccat gaacatttat ttcagaatag ttatatcact
      301 ggcaattatc gcatgtattt acggattaat cgttccggcc ctcatatcaa tgaaggatac
      361 ggtagcagtg atttctggct ttgctctggc gtgtctgacc ccgccctgca tttatgccat
      421 ttataagggt ctttcttttt ctaaggataa aagatgaaaa aagttattcc tgctttagcc
      481 cttgttctgc tgaccaccgg acttgtcggt tgcgatcgcg ttgagcctgg taatgttggc
      541 atcaaggtaa acaaactggg cgacgataaa ggcgtcggag aagtggtcgg tgttgggcgc
      601 tactggacgg gatggaacac tgaggtttac atcttcccga ccttcaaaca aatgaagacc
      661 tacgatgagc cattcagctt ccagatgagt gacggtacaa ccatcggcta tcacatcggc
      721 gtggcctaca aagttgatcc atccaaagtt accacagtct ttcagaccta ccgcaaaggc
      781 gtggacgaca ttaccgacac cgacctgcgc cagaagatag ccgacgcact caaccgactg
      841 gccagcaaaa tgaccaccga caaatttatc gacggcggca agtctgagct gctggatgca
      901 gctcttaaag acattcagga agaaatgacg cccatcggta ttcaggtaat gagcctctca
      961 tatgtgggta agccggagta cccgcctact gttatcgaca gcattaatgc caaagtcacg
     1021 gcgaaccaga aaaccctgca atgcgagcag gaagtaaaac agcgcgaagc ggaagccaac
     1081 atgttgcgcg cggaagctgc cggacaggca gatgcgattc gcacaaaagc ccaggccgaa
     1141 gccgatgcta ttcgtttacg cggtgaagct ctgcgccaga acccaaacgt tatggagctg
     1201 gaagccatca acaagtggaa cggtacactg ccgcaataca tgaccagcgg tgccaacaca
     1261 ccatttattc aggttaagta atacatatgc ccggtattac acgccgggct gtctggagat
     1321 aaaaatgaat actgtaacca tcaacaacaa acagtttccg gtaatcgaat atcgcggtca
     1381 gcgtgttgtg acgctggcga tgattgatga agtccaccag cgaccggaag gaaccgcgcg
     1441 tgcagcattc aaccgaaacc gtgagcattt catcagcggt gtggattatg ccgaattagg
     1501 tgcggacgta atacgtacgg acctcccgga aggaacattc tctaaatttg caccatcggg
     1561 gattgtactt tttgaatcag gttacctgat gttgacgaag ccattcaatg acgcccttgc
     1621 ctggcaggtt cagcgcgaac tggttaacag ctacttccgc actcgcacac cgctaacgga
     1681 aatcgagatg atcgctgcga tagccgccga cgctgttcgt caacagaagc gcctgaatca
     1741 tgtggaagaa cagctcgaaa cagtcacaga agcggtggaa accatcaaac gcggaaatat
     1801 gcgcgccgga tatgtcggtt accgccaggt ggtagccaaa agtggaatga gtgacaccaa
     1861 gtgtcggaat ctggtgaacg catatcaaat tcctacagac acccacgaat tcatgactcc
     1921 ggacggtctg ttgtctcgca gggctatcgt cgagcttgag ccatttatgg cggcgttcca
     1981 ccaaatgatg tcagaggctg acccacgcgg cacacgctgg tatcacccga agatggggct
     2041 tttccaggtg atcggatggg aggataaagc atgatcatcc agtcaaaact tattcgcgcc
     2101 gctctggtgt gcgctgctaa aaacgacgtt cgttattacc tgaacggtct tcacatcacg
     2161 ccgaaacata ttgaggcaac caatggttcc gtggcactgc gcatggctca cggcatccgg
     2221 acgaagaaaa acatcattgt ccagttcgaa gggggcgtcc cggccaaagc cgaaacgaca
     2281 gagctgattt ttagtaaaga gccgatcgct gttcatcgcg accagtttca gcgacgactg
     2341 tccattaccg gcattaaact ggtggacggt tgttttccgg atttggatcg catcattccg
     2401 aaaaagtttg accgctgtac tcacccggtg ctccaggcgg gttacctgag ttatccagag
     2461 aagatgtttg gtcgtgagcg taaatttatt cccgtccagt tacgtccctc cggtgacggg
     2521 caagcggtca gaattcagtt tgattccatc atcaactcaa tgtatggcaa tcctgaattt
     2581 gttgtgatgc cttgtcgtga tcatggcgat ttcaacgtgg ctcaggagca tccggaatga
     2641 aaatcgaata ccaggacgcc gcaggaggtg aatcaatgag ctggcctgat gcaatcgtaa
     2701 ctctggggat ggtattcgca gtagcgtttg ttgtgtactc gatttgtcga tggggataac
     2761 cacatgttcg ctttgattca acgcgggcag atatacacag acagagccgg atacccagta
     2821 gaaattactc gcagtactga gcactcagtg ttctttcgga ggatggacgg acgtaccggg
     2881 cgagtacgca tcggtgaatt cagtagcctg ttcgagcata ttgaccacct ggaatatcac
     2941 aagattctgg ctgaaacaga acaagaaaag cacctgaaga aattacgcgc aatgcaaagg
     3001 aagtaaaaaa tgaataaagc gtttgagcta tgggtccacc agcgttacgg caaccactac
     3061 gacctgacgc gagatgttga cggtttctac tgtcgtgaaa ttgtgaaacg aatgtttgag
     3121 gtctggtgcc actgccgtgg gctgagtgtt gtgtgaggta atgcatgggc aatgtgattc
     3181 aactggctcc caatgaatgg gtttgtgaaa gcgttctaat cgcaattacc gggctcaaac
     3241 caggcacaat tcttcggacc cggaaagaat gctggatggt tggaagagag tatattcacg
     3301 tatcaccaga cggtaatcca aagc
//
LOCUS       DAJTSA010000209         3187 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000209 DAJTSA010000000
VERSION     DAJTSA010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.210"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            168..1271
                     /gene="proB"
                     /locus_tag="OO369_004132"
     CDS             168..1271
                     /gene="proB"
                     /locus_tag="OO369_004132"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR5984174.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1283..2536
                     /gene="proA"
                     /locus_tag="OO369_004133"
     CDS             1283..2536
                     /gene="proA"
                     /locus_tag="OO369_004133"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR5984175.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2651..2726
                     /locus_tag="OO369_004134"
     tRNA            2651..2726
                     /locus_tag="OO369_004134"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2684..2686,aa:Thr,seq:cgt)
     gene            complement(2741..>3187)
                     /locus_tag="OO369_004135"
     CDS             complement(2741..>3187)
                     /locus_tag="OO369_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5984176.1"
                     /translation="ESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGT
                     KAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWE
                     SIEMVRRYAHLAPNHLTEHARQIDLILNPSVPNLSQSRNKEGTNDV"
BASE COUNT          752 a          807 c          877 g          751 t
ORIGIN      
        1 tggtaattta ttaaatctgt aataaaagcg taaacaactg ccgctacgct tgctgatccc
       61 gcgcaacaaa acgccatgct ttgctcgcag atggttggca accgacgaca gtcctgctaa
      121 aacgttcgtt tgatatcatt tttcctaaaa ttgaatggca gagaatcatg agtgacagcc
      181 agacgctggt ggtaaaactc ggcaccagtg tgctaacagg cggatcgcgc cgtctgaacc
      241 gtgcccatat cgttgaactt gttcgccagt gcgcgcagtt acatgccgcc gggcatcgga
      301 ttgttattgt gacgtcgggc gcgatcgccg ccggacgtga gcacctgggt tacccggaac
      361 tgccagcgac tatcgcctcg aaacaactgc tggcggcggt agggcagagt cgactgattc
      421 aactgtggga acagctgttt tcgatttatg gcattcacgt cgggcaaatg ctgctgaccc
      481 gtgctgatat ggaagaccgt gaacgcttcc tgaacgcccg cgacaccctg cgtgcgttgc
      541 tcgataacaa tatcgttccg gtaatcaatg agaacgatgc tgtcgctacg gcagagatta
      601 aggtcggcga taacgataac ctttctgcgc tggcggcgat tctggcgggt gccgataaac
      661 tgttgctgct gaccgatcaa aaaggtttgt ataccgctga tccgcgcaga aatccgcagg
      721 cagaactgat taaagatgtt tacggcattg atgacgcact gcgcgcgatt gccggtgaca
      781 gcgtttcagg cctcggaact ggcggcatga gtactaaatt gcaggccgct gacgtggctt
      841 gccgtgcggg tatcgacacc attattgccg cgggcagcaa gccgggcgtt attggtgatg
      901 tgatggaagg catttccgtc ggtacgctgt tccatgccca ggcgactccg cttgaaaacc
      961 gtaaacgctg gattttcggt gcgccgccgg cgggtgaaat cacggtagat gaaggggcaa
     1021 ctgccgccat tctggaacgc ggcagctccc tgttgccgaa aggcattaaa agcgtgactg
     1081 gcaatttctc gcgtggtgaa gtcatccgca tttgcaacct cgaaggtcgc gatatcgccc
     1141 acggtgtcag tcgttacaac agcgatgcat tacgccgtat tgccggacac cactcgcaag
     1201 aaattgatgc aatactggga tatgaatacg gcccggttgc cgttcaccgt gatgacatga
     1261 ttacccgtta aggagctggc cgatgctgga acaaatgggc attgccgcga agcaagcctc
     1321 gtataaatta gcgcaactct ccagccgcga aaaaaatcgc gtgctggaaa aaatcgccga
     1381 tgaactggaa gcacaaagcg aaatcatcct caacgctaac gcccaggatg ttgctgacgc
     1441 gcgagccaat ggccttagcg aagcgatgct tgaccgtctg gcactgacgc ccgcacggct
     1501 gaaaggcatt gccgacgatg tacgtcaggt gtgcaacctc gccgatccgg tggggcaggt
     1561 aatcgatggc ggcgtactgg acagcggcct gcgtcttgag cgtcgtcgcg taccgctggg
     1621 ggttattggc gtgatttatg aagcgcgccc gaacgtgacg gttgatgtcg cttcgctgtg
     1681 cctgaaaacc ggtaatgcgg tgatcctgcg tggtggtaaa gaaacctgtc gcactaacgc
     1741 ggcaacggtg gcggtgattc aggacgccct gaaatcctgt ggcttaccgg cgggtgccgt
     1801 gcaggcgatt gataatcctg accgtgcgct ggtcagtgaa atgctgcgta tggataaata
     1861 catcgacatg ctgatcccgc gtggtggcgc tggtttgcat aaactgtgcc gtgaacagtc
     1921 gacaatcccg gtgatcacag gtggtatagg cgtatgccat atttacgttg atgaaagtgc
     1981 agagatcgct gaagcattaa aagtgatcgt caacgcgaaa actcagcgtc cgagcacatg
     2041 taatacggtt gaaacgttgc tggtgaataa aaacatcgcc gatagcttcc tgcccgcatt
     2101 aagcaaacaa atggcggaaa gcggcgtgac attacacgca gatgcagctg cgctgacaca
     2161 gttgcaggca ggccctgcga aggtggtggc ggttaaagcc gaagagtatg acgatgagtt
     2221 tctgtcatta gatttgaacg tcaaaatcgt cagcgatctt gacgatgcca tcgcccatat
     2281 tcgtgaacac ggcacacaac actccgatgc gatcctgacc cgcgatatgc gcaacgccca
     2341 gcgttttgtt aacgaagtgg attcgtccgc tgtttacgtt aacgcctcta cgcgttttac
     2401 cgacggcggc cagtttggtc tgggtgcgga agtggcggta agcacacaaa aactccacgc
     2461 gcgtggccca atggggctgg aagcactgac cacttacaag tggatcggca ttggtgatta
     2521 caccattcgt gcgtaaataa aaccgggtga tgcaaaagta gccatttgat tcacaaggcc
     2581 attgacgcat cgcccggtta gttttaacct tgtccaccgt gattcacgtt cgtgaacatg
     2641 tcctttcagg gccgatatag ctcagttggt agagcagcgc attcgtaatg cgaaggtcgt
     2701 aggttcgact cctattatcg gcaccattta aatcaataag ttacacatca ttagtacctt
     2761 ccttatttct tgactgggac aaatttggga ccgatgggtt cagaatcaag tctatttgcc
     2821 gtgcgtgttc ggtaaggtga ttaggtgcga ggtgagcata tcgacgaacc atttcgatag
     2881 actcccagcc tcccatttcc tgtaacactg ataacgggac tccggcttga accagccaac
     2941 ttgcccaggt gtgtctcaag tcgtgaaatc tgaaatcatc aataccagcc cgtctcagcg
     3001 ccgctttcca ggctgtgttt gcgtcatacc gcatcttcct tactgttggc gctttcgttc
     3061 cgtctggttt ggtacagctt tccttgtaca caaataccca acggtgatga tttccgattt
     3121 gttttttcaa tacgcgacat gcagtatcat tcagcgcaac gccaattgcg cggtttgatt
     3181 tactctc
//
LOCUS       DAJTSA010000210         3180 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000210 DAJTSA010000000
VERSION     DAJTSA010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.211"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            490..684
                     /locus_tag="OO369_004136"
                     /pseudo
     CDS             490..684
                     /locus_tag="OO369_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
     gene            complement(1709..2818)
                     /gene="fepE"
                     /locus_tag="OO369_004137"
     CDS             complement(1709..2818)
                     /gene="fepE"
                     /locus_tag="OO369_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR5984177.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
BASE COUNT          946 a          775 c          658 g          801 t
ORIGIN      
        1 aaaaaaaaca caccgcgccg ctagcagcac ccctaatata agataatgtt ttttttatga
       61 aaatagtcag taccacccct aaaaagcggt gttggcgcag ctacaacacc gcgccgacac
      121 cgctttttta aatatcacaa agagagcaag aagaactaat ttgtcataac tctctattta
      181 taaagaaaaa tcagcaaaaa cttgtttttg cgtggggtgt gggcatttca ggcatgagaa
      241 cagactcgcg tttttccgga gtatcagcgg aattattgat taagaaacac tcgacgcaga
      301 gcgaaatttt atgcaacagc ggctgcactg cacagggatt atttatcagt tgatatgctg
      361 tgccatgata cgtcctaaag aaacaggaca ggatgtgcaa ttgtaatacc gtcgcccgca
      421 acgtcttaca attaccacct gctcagggtc ccccccccga cacccggaac tgacggagaa
      481 caacggaagg tgaacaaata acaacaaact gcgctgaata tggcgcgatg tatcaaaagc
      541 cagagcctga cactgcttga aaaactggat gctctggatg ccgacgaaca ggcggccatg
      601 tgcaaacgac tgcacgaact cgcagaagaa ctccagaaca gcatccagat tcgctttgag
      661 gccgaaagtg aaacaggaac ataacgaagc tgacggaggc ggtcacggct ttcccgtggg
      721 cgttttcgtc agcagacaag cccgtcaggg cgcgtcaggg ggttttagcg ggtgtcgggg
      781 cgcagccatg acccagtcac gtagcgataa cggagtgtat actggcttaa ttatgaggcg
      841 gcagagcaga tggtgagaga acagcgacat atgaggtgtg aaatgctgca cagatacgta
      901 aggagaaaat gcagcttccg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
      961 gtcgttcggc tgcggcgagc ggtgtctgct cacgcaaaga cggatatacg gttatccaca
     1021 gaatcagggg ataacaccag aaaaaacatg tgagcaaaaa agaggccccg aaaaaaggcc
     1081 gcgccggtgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     1141 tcaagtcaga ggtggcgaaa cccgacagga cttaaagata ccaggcgttt ccccctggtg
     1201 gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat
     1261 ggccgcgttc gtctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag
     1321 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1381 cgtcttgagt ccaacccggt aagacacgac ttaacgccat tggcagcggc cactggtaaa
     1441 caggatgtgc gagagatgta ttgagagttc ttgaagtggc agccttactg aggctacact
     1501 agaaggacag atttggtgac atgtctcgca caagactgtt accacggttc agaagttccc
     1561 caacttactg aaccttcgaa aaaccacctc cccaggtggt tttttcgttt tctagagtag
     1621 aagactacgg ctgtaaggga atgatctcaa gaagatcctt tgaacttttt ataaaagttg
     1681 cttataaaat gatgcatcag cacaactttt actcaatcac actcatcttt ctggcctcca
     1741 tggcatgacg taaaagtata tagccacatg caagcatccc tccaatgaca gaagaaagaa
     1801 ttacaattat ggcattactt ggcccatcct ttctgaccgg taacgttggg ctcaattgat
     1861 actgaaatgg cataaaatca acatccccca ctttcgccac aaccaattga ttcacaacat
     1921 attgacgatt tcgcaactcc ccactcaatt ccgaaacatc cttgattgat tttttgatgt
     1981 tcaatttagt tgctatacca tcagaaccga gagccacagg aaaatctggg tcatccttca
     2041 taatctgacc attgctgtat acaggttttt ttattccagc cgcattagca acctccagtg
     2101 aataattgag gcgtcgaata tctgcattca gctggtttct aatttttatc tcatccagtg
     2161 caagaatttc tttttcaaca ttagttttaa cttctagttt atttcttata tcttccatca
     2221 aatcccttac aacaagggaa gaaacataat taatataccc agacaacaca tcgtgagctt
     2281 ctttacttgt tggagcagta aaactcagag tccatgatac atacaatgat gacttgtcgt
     2341 ttttcttact ggcattatca tcaatcgact tcatattttt actcaactca acaattgcac
     2401 gatgcagttc gagagcgcta atgttattct ttttaataat ctccataaca taaggagatg
     2461 atctcatata ctcattcaac gaattaacgg actgaaattt cttaataaac agagcaaatg
     2521 cgctatcacg attgatatca aaatccactc ccagaacatg aagcttggaa agctccttat
     2581 ttaattcact ccattgaaca gactcagcag gagtaaccac agcagaacta gtccattttt
     2641 gtggcaaaac aaaagaaaca agaaagccaa tacaagcaaa aaatagaatc accccaacaa
     2701 tttttatttt tgctgcccat aaaacgctaa tcaagccaaa aaggtcaatt tcttcattac
     2761 cattcggcaa catgttgtaa ttagaaaaat gttcgtcaga gacctttgag attttcattg
     2821 aaaaaaacac aatatattaa catatggttt ttttattcta tcatatgtta ggaaaaaacc
     2881 taaaaaccat tcagcagaca tttaacataa cattttattg cgcgcaccgc taaaacgcgc
     2941 tgaacgcgat tctgccgcaa acaccgcaaa aagagatgtt ttccccgggg taaaccggaa
     3001 aaatgcgtca ggatcgcttt cagctcactg catagctatg catgaaagtg aatgacgatc
     3061 ggtttggccg catacagtgc ccatgagaca cattttcgga cattttcagt tcgtgaatcc
     3121 ctgactcatt gctacatatc tgaacagaca cccctagcag cgcccccagg ggcgtcctgt
//
LOCUS       DAJTSA010000211         3148 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000211 DAJTSA010000000
VERSION     DAJTSA010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.212"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..442
                     /locus_tag="OO369_004138"
                     /pseudo
     CDS             1..442
                     /locus_tag="OO369_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1026..1742
                     /locus_tag="OO369_004139"
     CDS             1026..1742
                     /locus_tag="OO369_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984178.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2294..2698
                     /locus_tag="OO369_004140"
     CDS             2294..2698
                     /locus_tag="OO369_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984179.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2761..3148
                     /locus_tag="OO369_004141"
                     /pseudo
     CDS             2761..3148
                     /locus_tag="OO369_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          885 a          642 c          713 g          908 t
ORIGIN      
        1 gcgttctaag ccttcccacc atgatcgggg aagccccaga tatgaacagg cacattcaga
       61 gttactttaa agagcttact gaacgtctga ggtgttagag ccatgactgc cgcagcattg
      121 ctggcggaaa tgcctgaatc gagcagcctc tcgagacgtg aaatcagcgc acttgtcggt
      181 gttgctcagg taaaccggga ctcgggaact ctgcgcggtc gacgaaccat tttcggtgat
      241 tgtgctgggg aagaacagct ctgtacatgg cggcgcttga ggcccccccc ggtttaatct
      301 ggtgataaag gcgttttaca tgcgcctact tgcagccgga aacgccaaaa aagtggcact
      361 ggttgcctgt atgcgtaagt tgctgaccat tatgaacgca atgctcagaa aaaacgaaga
      421 atggaatgaa tcgtatttat gaaaactgga ttgtgggcca taaatattcc aaaaagatag
      481 cccgctccgt ttattaccgg aacgggcgag agcatgacat tgtctgttat tataatgaga
      541 ctgctgattc tacttatttt taatttcaag aaaagcgttt tctacaaaga attatgagat
      601 ttaatgtttt gtttagacaa tattatcatt ctgttcaata atgaaagtct tcatcaacaa
      661 caagtaaaaa caacgtccct ctcatcgaac cggacttgca gatttcccgc atccggctct
      721 cttgcaacac atcagacctt cacgttcggg cggttatggg gaaggtccag caagctgtat
      781 gagccctaac tgtttgaata ccagtagatt accagaaact tctatcgatt gaagacatta
      841 ccaactagcg ttactaaatg ttgaccaagt tttgttcact tgtctggttt tgatagtagc
      901 attctgcaga gtattttgat atgataaata tgcattaaca atgatattat tgttaatttc
      961 ctagcatgaa aattaaaaga tcaactttta tctcgaatat tttttacatt atcagttagt
     1021 ttcttatgaa tgacaattca ttgcttagaa attcttcatt attcatcgcc tatatgggat
     1081 gtgtagggtg ggtcagtgct tattcttatg gctggggaac ctctttttac tatggatttc
     1141 catggtgggt tgttggtgca ggtttggatg atgttgccag aagtttactc tatgctatca
     1201 tagtaatggg tatacttttt accggttggg ggattggtat tttatttttt cttcttataa
     1261 agaaaagaag taaaatacaa gacttgagtt tttttagact attttttgct attaccctac
     1321 tgtttttccc tgtaatattc gaacttttaa ttctcaaaca atatttcata cttccattat
     1381 cactttcatt cattattagt tcattggtta tttcaataat aattcgtata tatggacgga
     1441 tcttttctgt atcatgtttt tcagatatcc cttttgttag agaacaccgc attaagttaa
     1501 ttatggctgg ttttttggtt tatttttggt ttttttcctt tcttgttggc tggtataaac
     1561 ctcaattaaa aaaagaatat cagatgcttt gttataataa tagttggtat tatgttcttg
     1621 ctcgttatga ttcacggctc gttttatcta gttcatttaa agatgacagt aacagattct
     1681 taatatttaa tacagaacag tcgggatttt atgaaatcaa tgatgtttat gtcagaaagt
     1741 aataatcctc tcccacaaag tgaattaaat gaatatataa tgcgttcgaa gtatatgtat
     1801 tgagctgtca tactgagaaa caaaccttag ggcagcacaa ttaaagccag aaacaactat
     1861 gcaagaaagt tactaaataa gtgctcggaa cagtaaaaca ctcagggggg actcagtccg
     1921 gattatgtaa tcagataaat tgccaaacat cacaaatcac cagccggact gagtgatgcc
     1981 gagcattgca ccaatacccc gtgatgaacg atgcctgatg cagaaagctc cctataaaac
     2041 aaagaacaaa aactacgacc ggcgattcac cgtcatactg atgctgcatc gtggtgacac
     2101 tgtctgtcac tttcatgttg actgaacaac tctgctgcga agaaggtgta ccgccggcgg
     2161 gatttgattg tccggcgtct agcttccact tccctgatgg aactgaaagt tgttatggcc
     2221 ggtcagcttg ccctgtccgc aggacatgag gtgctgtgac ggacagcacc agtaacggaa
     2281 agtgacaatg aaaatgaata ctgcgctgat agttgcacta atgtgtatgt ggtatgcggt
     2341 acccgctgct gcaaaagaga ctttactggc tatgccacgt aattcaacag aacattgcta
     2401 tgcggagatt aatgtgcacg gaccatatgg ggtgtatttc cgagtggtcc cgcatccccc
     2461 cgggggaaag agctgggtcg aatgtaacag tgactactat tactctgata aaccaccggg
     2521 agtacagata ctcggaaccc gtgcagggtg tcgggtatac ggaatctgcg ggaccacctc
     2581 gacactgcat gtggctggaa gaggggttgt gtgtattaaa aatatatgca gtccacgtgg
     2641 gatgataatt cacaggatac ggaaacgtcc tgtagtggct gtaagcgatg agatgtgatc
     2701 ttacccagca atagtagaca cgcggctaag tgagtaaact ctcagtcaga ggtgactcac
     2761 atgacaaaaa cagtatcaac cagcaaaaaa acacgtaaac agcattcgcc tgaattttgc
     2821 agtgaagccc tgaagcttgc tgaacgcatc ggtgttgctg ccgcagcccg tgaactcagc
     2881 ctgtatgaat ctcagctcta tgcgtggcgc agcaaactgc agcaacagat gacctcgtct
     2941 gagcgtgaaa gtgaactgcc cgcctgaagc ggcaactggc ggagcaggca gaggaactgg
     3001 ctatcctcca aaaggccgcg acatactttg cgcagcggct ggtatacgtg gtgtcagcgt
     3061 cggacaggaa taagccctcg tcagcagttc cgtcagcact gcgacagcgt tgtcctcgcg
     3121 gcggccttta cccggtcaaa acagcgtt
//
LOCUS       DAJTSA010000212         3112 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000212 DAJTSA010000000
VERSION     DAJTSA010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.213"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OO369_004142"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OO369_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR5984180.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(971..1066)
                     /locus_tag="OO369_004143"
                     /pseudo
     CDS             complement(971..1066)
                     /locus_tag="OO369_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OO369_004144"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OO369_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR5984181.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OO369_004145"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OO369_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR5984182.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2163..3112)
                     /locus_tag="OO369_004146"
                     /pseudo
     CDS             complement(2163..3112)
                     /locus_tag="OO369_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          773 a          753 c          757 g          829 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaagg caacagatcg cgtacccggt
     1021 ttgccggcca gtcctggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattcg ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatactataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aacacctgac ggcaggtatc
     2161 actcacccgg tgaaggtaaa tccctcaaga ggcagcaact ctgccagtcg ctcctccggc
     2221 cattccggca gacgcgtcag gacgtccgtc agccaggcat gtggctccag accattacgt
     2281 tttgctgttt ccagaaggct cattatctgt gccgcccggt ttccggccat cagtgaaccg
     2341 gcgaacagcc acgctttccg tcccataaca accgggcgaa tggcacgctc gcagatattg
     2401 ttgtccaggg gcacagctcc gtcacccagg aagcagctca gttcatcgcg ccggttcaga
     2461 atatagttaa tcgccttatt taatggcccg tcgggcggac agcacggctc ctgctcttca
     2521 agccaggcaa acaggtcatt cactatcggc cgggagtacc gttatcgcca ctgccgtatt
     2581 ttttccacag ggcgctcacg gatgagtttc tcaatgcggt aaagacccgc gatttttctc
     2641 agctccacgc ctgcacgggg ctccctgctg gccttataca gatttgcgaa gccgcgccct
     2701 gcgtgactcc agcaacaggc tgatgtgatg ccggggtgat tatccgccag agatttgtac
     2761 acgctgtatc cgtcactcac cagcgtactg ccacaccagc actgcagcca ggtctcaggg
     2821 tatctgaggg cccgtccggt ctgcgagtca aaacagacga ccggcggacc gcttctttca
     2881 ccgctgacgt aagcacacag ccagccggag caggatttac cgcctttccg ggtatcaagg
     2941 agcctcaggg acgtttcatc tgcatgaata acatcccgtg tcagaagctc atggcgtagc
     3001 agtttcgcca gcggggccag tgcagctccg gcaacaccga ccatatccgc cattgtactg
     3061 acggggagct ccacacccat tcgggagaaa atgtgctgct ggcgatacag gg
//
LOCUS       DAJTSA010000213         3097 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000213 DAJTSA010000000
VERSION     DAJTSA010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.214"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(104..1813)
                     /locus_tag="OO369_004147"
     CDS             complement(104..1813)
                     /locus_tag="OO369_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR5984183.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2245..2964)
                     /locus_tag="OO369_004148"
     CDS             complement(2245..2964)
                     /locus_tag="OO369_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR5984184.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT          953 a          588 c          519 g         1037 t
ORIGIN      
        1 gccaattgag gtggttgcct gaaagttggc agttacccgg tcactacacc ttgttggagt
       61 gaacatatca aaaacaggta attacacaat ttaaattaca gtctcataaa aaatgattaa
      121 atctatttat tatttcaata aacgtttttt tcatcttctt tgaaaattgt tctgctatag
      181 tatccggttc cagattgttc tcttttatta ttctacaata ctctaatatt gtttctttgt
      241 ctctatttac tacagataca aatacaccag gaataccatc tttattagtt gcggacagca
      301 tttcgaccaa tcgtcttgtg gtaaaactat agttttttag aatattacaa aaggcacgta
      361 ttgtttcggt atggccatta taaaatgcca taaaaagtcc tgatgtaccg ttgggatttt
      421 ttatccataa taattcatct atatattcct ggtgaggggg gagtattttt agtatatctc
      481 catacgccat aatggccttt gtgtgcccat tttgaagtgc cataaacaac ccatgcttta
      541 ctttcaataa ttctttaatt tgttcactac taagcatttt cttattaagc agacttccga
      601 acatacgaat actatctgca tgtccattct gcaatgcaaa aaataaccca ggtaaatcat
      661 ggctgtcttt cgcagctaat agatataatc tcttatcgga agttaacggg gttttaaaga
      721 ctatttcccc ataggctttt attgtgtcta catgtccgtt cataaaagac atgcataaac
      781 ccggcacatt gtttgcccct accgcagcaa gcaatgtaag tattgtttct tgactaagat
      841 tgcacatatt taatatctta ccatatactt gaataatgtc tgaatatccg ttttgaagtg
      901 ctatataaaa tgcaggagta ccatcagagc ttctcgtggc cagaaggttc accagtttag
      961 attgagaaat ttcaccattt ttgtattttt ccatcagtat aaataatact tctgtcagat
     1021 tctttgttat cgcatgatat ataactctgg aattgatcaa ttcatctggt aaaagtttca
     1081 ctgtgtttgt tagaggggaa ttatgcctat cttcaaatat tacctgcccg ggttgctcca
     1141 gaccataaat tttccaataa aaatcatcaa aaaaatcctt agcggataat tgactcagtt
     1201 cgaaatggct atcacaagta aatacagtac gtttatgagt aacagttcga ttaggatcga
     1261 aaaactgaac aacccatttt tcactttctt gtacgcgctt tattttcaac cctaacgcca
     1321 tttggtggtt taatgtgcat gctgataatg ttctgacaaa gtctccattt tttttcatgt
     1381 tataaaaaaa attagtaata actgttcccc actttttatt ttccacaaaa taaatataag
     1441 ctggtgattt tgaaaaatct tccgtttttt ccaaacatac attttttgca atagcatcag
     1501 gggtgctaag aagctgatag tttaccttcc catttgtttc cataaaattc atttcccaat
     1561 aggaagctag gtgacggcaa actatttgtc ttccatatat atcttttgcc actccattta
     1621 tatttacctc cacatttctc ttttctgaaa aataaggtgc tgttttattc ttaaactgaa
     1681 ttagttcact ggagtttgag accctcataa tagaacttgt tattgttcta ttatctctgt
     1741 taacagacat atctgttgat aacgaatttt ttgttgaaaa tatactagat gaaaatgttt
     1801 tatttaacgg catagaagac aaccatagag atgttataat actattagat tgcagttact
     1861 aatagtaaaa gcctccttac aaggaggtag cttattcaga atgctcctat aagatagcga
     1921 gctcgtcaac gatacagatg atctgattat attgtgcaaa aggagaacag gaaaataggc
     1981 actggaaaag atgaaggcct gatgggagca ttaactaatg caaaatccga caggttaata
     2041 tcccgaaaag caatcaaacg actcaaacag gagatgcacg ctgaaatggt ggacaactgt
     2101 tcggatttca gatgagtagc tcagcagttt atgagccgaa tgctctgtca cgggcaggaa
     2161 tcaggctgca tacgcagtgc caaatctaat cgtagttatt ctaaccagtt ttcaagctac
     2221 aaggtggtgt agctggcatc ttctctactc tatcatcaaa cgataaaatg gttcctctcg
     2281 taacatttgc tcttgccttt cacttccatc tcgatcgctt cgtagttcat tacgatagct
     2341 gacatatttc cagtcttcag gtctaacatc tgacgccgga tactccccgg gagcgatttt
     2401 gcttcttaat agattagact cgagacacat tataaattgt cgtattttat gaagtaataa
     2461 agcactatat tgcgagcatt cctgatctga ttttacatat agcgtaaact gagctcctat
     2521 ccccacacga gattgctgag aaactcgatt catatcagtt atcttccatt tatctatagg
     2581 actgtcctct gaaaataata aaccagacaa aatttggaat gccagaggaa cttgctccct
     2641 agctatacta atatgaaact tgtccccaac aaaatccccc ttactctgag gattttcacg
     2701 acatgcatga ataaacacat ctccactatg gttcacaatc tggaatcctt ggtacatgac
     2761 agaaaaagat tgacatatat cagagtaagt tggcacgttc gatgctactt tgaaatagta
     2821 cgctgggtat tgccgtattt ggttgtacaa tatattgaag ttatttttca atctttcatt
     2881 cgcagaaagc atctgcggta tttcagacct cacaacattc aaagaatcta aatttagttt
     2941 tagacagggc ttttttatgg gcatagaaaa cgtcctctat aaaatagata tattatctta
     3001 tattgattgt ttatcatctt ttataaaacg atacattata tgtaatgtat gtaagcgtaa
     3061 gggtcctctc cgaacatggt gggaaggctt agaacgc
//
LOCUS       DAJTSA010000214         3069 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000214 DAJTSA010000000
VERSION     DAJTSA010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.216"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..498
                     /locus_tag="OO369_004149"
     CDS             <1..498
                     /locus_tag="OO369_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5984185.1"
                     /translation="DGEILKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTI
                     PLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRID
                     DAESGNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNV
                     TLDYR"
     gene            578..1273
                     /locus_tag="OO369_004150"
     CDS             578..1273
                     /locus_tag="OO369_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5984186.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1302..>3069
                     /locus_tag="OO369_004151"
     CDS             1302..>3069
                     /locus_tag="OO369_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5984187.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          888 a          718 c          684 g          779 t
ORIGIN      
        1 gatggcgaga ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt
       61 gctcttggtc actataatgc cgaacagttt cgttctgttg gcgatcgtag cccaaaaatt
      121 ccattcacta ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac
      181 gttgaagcgt ctttccgttt atggctggaa acgcgggata atggcaccgt ccctaacttc
      241 cctaatctgg caaaagtggg ctcttttgct ggcacagcga caacaggtgt gggtatccgt
      301 attgacgatg cggaaagcgg aaaccttatg ccactgaatg ctatggggaa tgataacacg
      361 atttatcaga tcccagcgga ctccaatggt attgtcaatg ttgacctcat cgcttactac
      421 gtatcaaccg ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta
      481 acgctggatt accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc
      541 cttcctcttt ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt
      601 gatattaacc ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt
      661 acagcgaacg cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa
      721 caaaggcgca gaaacgcctt atttactgca atcatgggta gataatatag atggtaaaag
      781 ccgtgcccca tttattataa ccccaccgct atttcgtctt gaggctagcg atgactcatc
      841 actgcgaatt attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat
      901 taatgttcgt gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac
      961 gctggtattt aaaacgcggg tcaaaatgtt ttatcgcccc gcacacctga agggacgggt
     1021 aaacgatgcg tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa
     1081 cccaactgaa tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag
     1141 caaaattgaa tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg
     1201 aaagaacgtg aaatgggctg cgatcaacga ttatggcggc agttccggga cagaaactcg
     1261 tccactgcaa taaaaaatat aaaaacacag gccatcaggg aatgccacaa cgacaccacc
     1321 agggacataa acgcacaccg aaacagttgg cgctcattat caaacgttgt ttgccgatgg
     1381 tgctcactgg cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc
     1441 ccattatgct ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa
     1501 aaaaatacgc acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg
     1561 tttcacagaa aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta
     1621 cggtagaaca actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa
     1681 aagatgacga tagcgtgatc aactcgcttg aacaaatcat tcccggtaca gctgctgaat
     1741 ttgatttcaa tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg
     1801 caagaggtta cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact
     1861 actcgtttac aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc
     1921 tgaatatgca aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga
     1981 cacgcaacga tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca
     2041 aggcgttgaa gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca
     2101 gctacgcctt tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc
     2161 gcggatttgc cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc
     2221 aaaatggtta tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc
     2281 tctacccctc ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacac
     2341 aacgtcgctt tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa
     2401 aatatagcgc gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg
     2461 aatttgctga agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc
     2521 tgctcggttc tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac
     2581 tgggcgcgtt gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt
     2641 ttcatggcta tcaatggcgt actcagtaca tcaaagatat cccggaaacc aacaccaata
     2701 tcgctgttag ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata
     2761 cccgcaattg gggctataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga
     2821 caatatttga tggggtaagt ctgtatgcct ccggttcaca gcaagactat tggggcaata
     2881 acgagaaaaa cagtaatatc tctgttgggg tttccggcca gcaatgggga attggttaca
     2941 gcctgaatta tcaatacagc cgctacactg atgaaaataa tgaccgtgca ctctctttga
     3001 atctcagtat tccgttagaa cgctggtgga tttgccccta tatttccaga catctgttat
     3061 cacttaacc
//
LOCUS       DAJTSA010000215         3064 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000215 DAJTSA010000000
VERSION     DAJTSA010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.217"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..197
                     /locus_tag="OO369_004152"
     CDS             <1..197
                     /locus_tag="OO369_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR5984188.1"
                     /translation="LVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQ
                     RGVNVFIQDVPGDQKEQIPD"
     gene            236..1039
                     /gene="agaC"
                     /locus_tag="OO369_004153"
     CDS             236..1039
                     /gene="agaC"
                     /locus_tag="OO369_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HCR5984189.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1029..1820
                     /gene="agaD"
                     /locus_tag="OO369_004154"
     CDS             1029..1820
                     /gene="agaD"
                     /locus_tag="OO369_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HCR5984190.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1821..2576
                     /locus_tag="OO369_004155"
     CDS             1821..2576
                     /locus_tag="OO369_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HCR5984191.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
     gene            2977..>3064
                     /locus_tag="OO369_004156"
     CDS             2977..>3064
                     /locus_tag="OO369_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5984192.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVF"
BASE COUNT          796 a          683 c          727 g          858 t
ORIGIN      
        1 aattggtaga aggtggcatt gacctgaaag atgtcaacgt cggcaatatg catttctcgg
       61 aagggaaaaa gcaaatcagc agtaaagttt atgtcgatga ccaggatctc gcggacttac
      121 gttttattaa acaacgtggc gtgaatgttt tcattcagga cgtccctggc gatcaaaaag
      181 aacaaatccc tgactaaatc taaaatcgcc ttaatattgg tttgaggtaa taaaaatgca
      241 tgaaataacc ctacttcagg gattatccct agcggcgtta gttttcgttc tggggattga
      301 tttttggctg gaagccttat ttttattccg cccgataatc gtttgtaccc taactggcgc
      361 tattctcggt gatattcaga ctggcttaat taccggtggt ctgacagagt tggctttcgc
      421 cggattaacc cctgcaggtg gtgttcagcc gcccaacccg attatggcgg gtctgatgac
      481 caccgtcatt gcatggtcta cgggcgttga tgccaaaaca gcaattggtc ttggcctgcc
      541 gtttagtttg ttaatgcagt acgtcattct gttcttctat tccgctttct cattatttat
      601 gaccaaagcc gataaatgcg cgaaagaggc ggatacggca gcgttttccc ggcttaactg
      661 gacaacgatg ctcatcgtcg cttcagcgta tgcggtgatt gctttcctct gtacttacct
      721 ggcacaaggg gcgatgcagg cgctggtgaa agcgatgccc gcctggttga cccacggctt
      781 tgaagtggct ggcggtattc tgcctgccgt tggttttggc ttgctgctgc gcgtaatgtt
      841 caaagcgcaa tatatccctt acctgatcgc cggtttcctg tttgtttgct acatccgggt
      901 cagcaacctg ttgccggttg ccgtactggg cgcaggcttt gcggtgtatg agtttttcaa
      961 tgcgaaatcc cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg aagaagagga
     1021 ctacagcaat gggatctgaa atcagtaaaa aagatatcac ccgtctgggc tttcgttcgt
     1081 cgctgctgca agcgagcttt aactacgaaa ggatgcaggc gggcggtttt acctgggcga
     1141 tgttgccgat cctgaaaaag atttataagg acgacaaacc gggcttaagc gcggcgatga
     1201 aagataacct cgaatttatt aatacccacc cgaatctggt cggattcctg atggggttat
     1261 taatttcgat ggaagaaaaa ggagaaaacc gcgacaccat taaaggcctc aaagtggcac
     1321 tgtttggccc aatcgccggg attggcgatg cgattttctg gtttactttg ttgccgatta
     1381 tggcgggaat ttgctcatca tttgccagcc agggaaacct gctggggccg attctatttt
     1441 tcgccgttta cctgcttatc tttttcctgc gcgtcggctg gacccacgtc ggttattcag
     1501 tcggcgtgaa ggcgatcgat aaagtgcgag agaactcgca gatgattgct cgctcggcaa
     1561 ccatcctcgg gatcacggta atcggcgggc tgatcgcttc gtatgtgcat attaacgtgg
     1621 tgacatcgtt tgccatcgac agtacccaca gcgtcgcgct gcaacaggat ttcttcgata
     1681 aagtcttccc gaacatttta ccgatggcct acaccctgct gatgtattac ttcctgcggg
     1741 tgaaaaaagc gcatccggtg ctgttaatcg gcgtgacttt tgtgctctct attgtttgtt
     1801 ccgcattcgg cattttgtaa atggaacgag gcactgcttc tggcggtgcc tctttactta
     1861 aggaatttca tcctgtgcaa acccttcagc aagttgaaaa ctatacggcg ttaagtgaac
     1921 gagccagcga atatttattg gctgtgatcc gtagcaaacc ggatgccgtg atttgcctgg
     1981 cgaccggagc cacgccatta ctgacgtatc attatctggt agaaaaaatc caccagcagc
     2041 aggttgatat cagccagctc accttcgtga agctcgacga atgggtggat ctgccattaa
     2101 cgatgcctgg cacctgcgaa actttcctac aacagcatat cgtgcagccg ctggggctac
     2161 gtgaagacca gctcatcagc tttcgctccg aagagataaa tgagacagag tgcgaacggg
     2221 taacgaacct gattgcgcgc aaaggcggtc tggatttatg cgttctcgga ttggggaaaa
     2281 acggtcatct tgggctgaac gaaccgggag aaagcctaca accggcctgc catatcagtc
     2341 aacttgatgc cagaacacag caacatgaga tgttaaaaac cacgggtcgc cccgtgactc
     2401 gtgggatcac cttaggcctg aaggatattc tcaatgcccg cgaggttttg ttattggtga
     2461 ctggcgaagg aaagcaggat gcgacagaac gttttctcac ggctaaagtc tccaccgcta
     2521 tcccggcttc atttttatgg ctgcacagta actttatttg tttaattaat acctaaccag
     2581 atgaaaaatt tgctcaaaat ttttaaatta acttattcaa cagtcacgca ttatattaaa
     2641 taacatttga ctgggttgaa cataacgcca atagcaaaag gagttatgtt caacaattta
     2701 attttagata tacatataac ttgttctatt aataaatcac ctcgtattct gactcggccc
     2761 ctggcgcagc aaagtcaggc atttatactc tttttaacac atagaaaatt catatcaata
     2821 taataaaaac ataaaatatt tgcatataca tataatctta aagtttagtc tatttaatgt
     2881 tcaatgaaat atttctacct gtataatctt taaagatgtt gaacatatat tcacattaaa
     2941 tatgattatg tacttgttac aaggataagg ttataaatga ataaagttac aaaaacagct
     3001 attgtgggct tacttgcgct tttcgcaggt aatgccgctg caaccgatgg tgaaatagtt
     3061 tttg
//
LOCUS       DAJTSA010000216         3051 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000216 DAJTSA010000000
VERSION     DAJTSA010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.218"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            2..2905
                     /locus_tag="OO369_004157"
     rRNA            2..2905
                     /locus_tag="OO369_004157"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          808 a          672 c          951 g          620 t
ORIGIN      
        1 aggttaagcg actaagcgta cacggtggat gccctggcag tcagaggcga tgaaggacgt
       61 gctaatctgc gataagcgtc ggtaaggtga tatgaaccgt tataaccggc gatttccgaa
      121 tggggaaacc cagtgtgatt cgtcacacta tcattaactg aatccatagg ttaatgaggc
      181 gaaccggggg aactgaaaca tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc
      241 cagtagcggc gagcgaacgg ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga
      301 agcgtctgga aaggcgtgcg atacagggtg acagccccgt acacaaaaat gcacatactg
      361 tgagctcgat gagtagggcg ggacacgtgg tatcctgtct gaatatgggg ggaccatcct
      421 ccaaggctaa atactcctga ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa
      481 agaaccccgg cgaggggagt gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag
      541 cacgcttagg cgtgtgactg cgtacctttt gtataatggg tcagcgactt atattctgta
      601 gcaaggttaa ccgaataggg gagccgaagg gaaaccgagt cttaactggg cgttaagttg
      661 cagggtatag acccgaaacc cggtgatcta gccatgggca ggttgaaggt tgggtaacac
      721 taactggagg accgaaccga ctaatgttga aaaattagcg gatgacttgt ggctgggggt
      781 gaaaggccaa tcaaaccggg agatagctgg ttctccccga aagctattta ggtagcgcct
      841 cgtgaattca tctccggggg tagagcactg tttcggcaag ggggtcatcc cgacttacca
      901 acccgatgca aactgcgaat accggagaat gttatcacgg gagacacacg gcgggtgcta
      961 acgtccgtcg tgaagaggga aacaacccag accgccagct aaggtcccaa agtcatggtt
     1021 aagtgggaaa cgatgtggga aggcccagac agccaggatg ttggcttaga agcagccatc
     1081 atttaaagaa agcgtaatag ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg
     1141 ctaaaccatg caccgaagct gcggcagcga cgcttatgcg ttgttgggta ggggagcgtt
     1201 ctgtaagcct gtgaaggtgt gctgtgaggt atgctggagg tatcagaagt gcgaatgctg
     1261 acataagtaa cgataaagcg ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc
     1321 caacgttaat cggggcaggg tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat
     1381 gggaaacagg ttaatattcc tgtacttggt gttactgcga aggggggacg gagaaggcta
     1441 tgttggccgg gcgacggttg tcccggttta agcgtgtagg ctggttttcc aggcaaatcc
     1501 ggaaaatcaa ggccgaggcg tgatgacgag gcactacggt gctgaagcaa caaatgccct
     1561 gcttccagga aaagcctcta agcatcaggt aacatcaaat cgtaccccaa accgacacag
     1621 gtggtcaggt agagaatacc aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa
     1681 tggtgccgta acttcgggag aaggcacgct gatacgtagg tgaagcgact tgctcgtgga
     1741 gctgaaatca gtcgaagata ccagctggct gcaactgttt attaaaaaca cagcactgtg
     1801 caaacacgaa agtggacgta tacggtgtga cgcctgcccg gtgccggaag gttaattgat
     1861 ggggttagcg caagcgaagc tcttgatcga agccccggta aacggcggcc gtaactataa
     1921 cggtcctaag gtagcgaaat tccttgtcgg gtaagttccg acctgcacga atggcgtaat
     1981 gatggccagg ctgtctccac ccgagactca gtgaaattga actcgctgtg aagatgcagt
     2041 gtacccgcgg caagacggaa agaccccgtg aacctttact atagcttgac actgaacatt
     2101 gagccttgat gtgtaggata ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag
     2161 ccgaccttga aataccaccc tttaatgttt gatgttctaa cgtggacccg tgatccgggt
     2221 tgcggacagt gtctggtggg tagtttgact ggggcggtct cctcctaaag agtaacggag
     2281 gagcacgaag gttggctaat cctggtcgga catcaggagg ttagtgcaat ggcataagcc
     2341 agcttgactg cgagcgtgac ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg
     2401 gttctgaatg gaagggccat cgctcaacgg ataaaaggta ctccggggat aacaggctga
     2461 taccgcccaa gagttcatat cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat
     2521 cctggggctg aagtaggtcc caagggtatg gctgttcgcc atttaaagtg gtacgcgagc
     2581 tgggtttaga acgtcgtgag acagttcggt ccctatctgc cgtgggcgct ggagaactga
     2641 ggggggctgc tcctagtacg agaggaccgg agtggacgca tcactggtgt tcgggttgtc
     2701 atgccaatgg cactgcccgg tagctaaatg cggaagagat aagtgctgaa agcatctaag
     2761 cacgaaactt gccccgagat gagttctccc tgacccttta agggtcctga aggaacgttg
     2821 aagacgacga cgttgatagg ccgggtgtgt aagcgcagcg atgcgttgag ctaaccggta
     2881 ctaatgaacc gtgaggctta accttacaac gccgaagatg ttttggcgga ttgagagaag
     2941 attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaattag
     3001 cctggcggcc gtagcgcggt ggtcccacct gaccccatgc cgaactcaga a
//
LOCUS       DAJTSA010000217         2953 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000217 DAJTSA010000000
VERSION     DAJTSA010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.219"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            143..2011
                     /gene="kup"
                     /locus_tag="OO369_004158"
     CDS             143..2011
                     /gene="kup"
                     /locus_tag="OO369_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR5984193.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2178..2597
                     /gene="rbsD"
                     /locus_tag="OO369_004159"
     CDS             2178..2597
                     /gene="rbsD"
                     /locus_tag="OO369_004159"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR5984194.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2605..>2953
                     /locus_tag="OO369_004160"
     CDS             2605..>2953
                     /locus_tag="OO369_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5984195.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          659 a          702 c          779 g          813 t
ORIGIN      
        1 caaagggggg gctttttctt tgagccaggt taatgtggcc gcatttagga gtacgatttt
       61 gccgttaatc gtgcatactg tgcgcttttt tgtgggccaa gggactaagc acacatttca
      121 tatttcaacg aaagactagt ctatgagcac tgataataag caatcattgc ccgcgattac
      181 cctcgcggcg attggagttg tctacggcga tattggtacc agcccgttat atacacttcg
      241 tgaatgtttg tccggccagt ttggttttgg cgttgaacgc gatgccgtgt ttggcttttt
      301 atcgctgatc ttctggctgc taatctttgt ggtttccatt aaatatctca ccttcgtgat
      361 gcgggcagat aacgccggtg agggggggat cctgacgttg atgtcgcttg ccgggcgtaa
      421 tacgtcggcg cgaaccacat caatgctggt gattatgggg ctaatcggcg gcagcttttt
      481 ctatggtgaa gtcgtcataa cacccgctat ttcggtgatg tcagccattg aaggtctgga
      541 aatcgtcgcc ccgcagctgg atacctggat agttcccctc tcaattatcg ttctcacttt
      601 attatttatg attcaaaagc atggcacggc tatggtcggt aagctgtttg cgccgatcat
      661 gctgacctgg tttttgattc tggcaggact ggggttacgt agcattattg ctaacccgga
      721 agtgctgcat gcgctgaatc cgatgtgggc ggtgcatttc ttccttgaat acaaaacggt
      781 ttcttttatt gcattagggg cagtggtgct gtcgattacg gggggcgagg tgctgtatgc
      841 tgatatgggg cactttggta agttctctat tcgcctggcg tggttcaccg tcgtattacc
      901 ttccttgacc cttaattact tcggtcaggg agcgttgttg ttaaagaacc cggaagcgat
      961 taagaacccg ttcttcctgt tggcaccgga ctgggcgctg atcccactgc tgatcatcgc
     1021 cgcactggcg acggtaattg cctcgcaggc ggttatctct ggcgtcttct cattgacgcg
     1081 tcaggcggta cgtctgggat atttgtcgcc gatgcgcatt attcacacct ccgaaatgga
     1141 gtcagggcaa atctatattc cgtttgtgaa ctggatgctc tatgtcgcgg tcgtgattgt
     1201 gattgtcagc tttgagcact ccagcaacct ggcggcggcg tacgggattg cggtgaccgg
     1261 aaccatggta ctgacgtcta ttctctcgac taccgtggca cgtcagaact ggcactggaa
     1321 taagtatttt gttgcgctga tcctgattgc tttcctttgt gtcgatattc cattgttcac
     1381 cgctaacctc gataaactgc tctccggcgg ctggttgcca ttgagcctcg gtactgtgat
     1441 gtttatcgtg atgaccacct ggaagagcga gcgtttccgc ttgctgcggc ggatgcatga
     1501 acatggtaac tctctggaag cgatgattgc ttcgctggag aaatcaccgc ctgttcgcgt
     1561 gcccgggacc gcggtgtata tgtcgcgtgc aatcaacgtc attccctttg cgctgatgca
     1621 taaccttaaa cataacaagg tattgcatga gcgggtgatt ctgttaactc tgcgcaccga
     1681 agacgcgcca tatgtccata acgtccgtcg ggtacagatt gaacaactgt cgcccacttt
     1741 ctggcgcgtg gtggcaagtt atggttggcg agaaacgcca aacgtggaag aagttttcca
     1801 ccgctgcggt ctggaaggat taagttgccg gatgatggaa acctccttct ttatgtcgca
     1861 tgagtcgttg atcctcggca aacgcccgtg gtatttgcgt ctgcgcggca agctgtactt
     1921 gctgctgcaa cgtaatgcgc tgcgtgcacc agatcaattt gaaatcccgc caaacagggt
     1981 tatcgaactg ggtactcagg tcgaaatcta acgccagacg cctcctttct tcataagggg
     2041 gcgtttttgt tttcatggtt aatcaccatg taaaacgttt cgaggttgat cacatttccg
     2101 taacgtcacg atggttttcc caactcagtc aggattaagc tgcggatcag cgaaacgttt
     2161 cgctgatgga gaaaaaaatg aaaaaaggca ccgttcttaa ttctgatatt tcatcggtga
     2221 tctcccgtct gggacatacc gatacgctgg tggtgtgtga tgctggttta cccatcccca
     2281 aaagtacaac gcgtatcgat atggcattaa cccaaggtgt accttctttt atgcaggtgc
     2341 tgggcgtcgt cacaaatgaa atgcaggtcg aggctgccat tatcgcggaa gagatcaaac
     2401 aacataatcc gcaactccac gaaacgttgc tcactcacct tgagcagctg caaaaacacc
     2461 agggaaatac cattgaaatt cgttacacca cgcatgaaca attcaaacaa caaaccgcag
     2521 aaagtcaggc ggtaattcgc agcggagaat gttctccgta tgcgaatatc attctctgtg
     2581 ctggcgtgac gttctgaggc cgtcatggaa gcattacttc agcttaaagg catcgataaa
     2641 gccttcccgg gcgtaaaagc cctctcgggc gcagcgttaa atgtctatcc gggccgtgtg
     2701 atggcgctgg tgggcgaaaa cggcgcgggt aaatccacca tgatgaaagt gcttactggt
     2761 atctatactc gcgatgccgg cacgctttta tggctgggga aagaaacgac atttaccggg
     2821 ccgaaatctt cccaggaagc cgggattggg attatccatc aggaactgaa cctgatcccg
     2881 cagttgacca ttgccgaaaa cattttcctc ggtcgtgagt ttgttaatcg ctttggcaaa
     2941 attgactgga aaa
//
LOCUS       DAJTSA010000218         2932 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000218 DAJTSA010000000
VERSION     DAJTSA010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.220"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            75..650
                     /gene="ssuE"
                     /locus_tag="OO369_004161"
     CDS             75..650
                     /gene="ssuE"
                     /locus_tag="OO369_004161"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR5984196.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            643..1602
                     /gene="ssuA"
                     /locus_tag="OO369_004162"
     CDS             643..1602
                     /gene="ssuA"
                     /locus_tag="OO369_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR5984197.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1599..2744
                     /gene="ssuD"
                     /locus_tag="OO369_004163"
     CDS             1599..2744
                     /gene="ssuD"
                     /locus_tag="OO369_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCR5984198.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2753..>2932
                     /locus_tag="OO369_004164"
     CDS             2753..>2932
                     /locus_tag="OO369_004164"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter"
                     /protein_id="HCR5984199.1"
                     /translation="MMATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPE
                     GVVTAFWTLSASSELW"
BASE COUNT          660 a          800 c          805 g          667 t
ORIGIN      
        1 tgtctctccg acagactgac cgaattgtta tcaatgttaa caaaaaaaag aacaattggt
       61 tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct
      121 ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctagatgta gaggtttatc
      181 actggaatct gcaaaacttc gccccggaag atctgcttta tgctcgtttc gatagtccgg
      241 cactcaagac cttcaccgaa cagctgcaac aggctgatgg gctgattgtc gccacgcctg
      301 tgtataaagc cgcctattcc ggcgcgctga aaaccctgct cgacctgctg ccagagcgcg
      361 ctttgcaagg caaagtggtg ctaccgctgg cgacgggcgg taccgtggcc catctgctgg
      421 cggtcgatta tgcccttaaa ccagttttaa gcgcactgaa agctcaggag atcctgcacg
      481 gcgtgtttgc cgatgactca caattaattg attaccatca cagaccccag ttcacgccaa
      541 atctgcaaac ccatcttgat accgcgctag aaactttctg gcaggcattg caccgccgcg
      601 atgttcaggt tcctgacctt ctgtctctgc gaggtaatgc ccatgcgtaa catcattaaa
      661 ctggcgctgg cgggattgct tagcgtctct acttttgcgg ttgctgcaga atcctcgcct
      721 gaagcgttac gtataggcta tcagaaaggc agtattggta tggtgctggc aaaaagccac
      781 cagttactgg aaaaacgcta tccgcaatca aaaatctcct gggtggagtt ccccgcgggt
      841 ccgcaaatgt tggaagcgtt aaacgttggc agtattgatc tcggcagtac cggggatatt
      901 ccgccaatct ttgcccaggc tgccggggct gatttggtat atgtgggcgt cgagccaccg
      961 aagcccaaag ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac cgtagccgat
     1021 cttaaaggtc acaaagttgc ctttcagaaa ggttccagtt cacacaacct tttactgcgt
     1081 gcactgcgtc aggccggact taaattcacc gatatccagc ccacttacct gacgcctgcc
     1141 gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg ggatccctac
     1201 tactccgctg cgttattaca gggcggcgtg cgggtgctga aagacggcac cgatctcaat
     1261 caaactggat cgttttatct ggcagctcgt ccctatgcag aaaaaaacgg cgcttttatt
     1321 cagggcgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca gcgcgaacaa
     1381 agcatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc ctcttactta
     1441 gatcatcgcc ctcctaccac catcaaaccg gttaacgccg aggttgccgc cttacagcag
     1501 caaacggcag atctgtttta tgaaaaccgt ctggtgccga aaaaagtcga tattcgccag
     1561 cgcatctggc agcccactca actggaagga aaacaattat gagtctgaat atgttctggt
     1621 ttttaccgac ccacggtgac gggcactatc tgggaacgga agaaggttca cgcccggttg
     1681 atcacggtta tctgcaacaa attgcgcaag cggcggatcg tctgggctat accggtgtgc
     1741 tgatcccaac ggggcgatcg tgtgaagatg cgtggctggt tgcagcgtcg atgatcccgg
     1801 tgacgcagcg gctgaagttt cttgtcgccc tgcgtcccag cgtaacctca cctactgttg
     1861 ccgcccgcca ggccgccacg cttgaccgtc tctcaaatgg tcgtgcgttg tttaatctgg
     1921 tcacaggcag cgatccacaa gagctggcag gcgacggcgt gttccttgat catagcgagc
     1981 gctacgaagc ctcggcggaa tttacccagg tctggcggcg cttattgctg ggggaaaccg
     2041 tcaatttcaa cggtaaacat attcatgtgc gcggagcaaa actgctcttc ccgccgattc
     2101 aacagcctta tcctccgctt tactttggcg gatcgtcaga tgtcgcccag gagctggcgg
     2161 cagaacaagt tgatctctac ctcacctggg gcgaaccgcc ggaacttgtt aaagagaaaa
     2221 tcgaacaagt gcgggcgaaa gctgccgcgt atgggcgcaa aattcgtttc ggtattcgtc
     2281 tgcatgtgat tgttcgtgaa actaacgacg aagcgtggca ggccgccgag cggttaatct
     2341 cgcatcttga tgatgagact atcgccaaag cacaagccgc attcgcccgg acggattccg
     2401 taggacaaca gcgaatggcg gcgctacata acggcaagcg cgacaatctg gagatcagcc
     2461 ccaatttatg ggcgggcgtt ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg
     2521 atggtcctac ggtcgccgcg cgaatcaacg aatacgccgc gcttggcatc gacagctttg
     2581 tgctttcggg ctacccgcat ctggaagaag cgtatcgggt tggcgagttg ctgttcccac
     2641 atctggatgt cgccattccg gaaattcccc agccgcagcc gctgaatccg caaggcgaag
     2701 cggtggcaaa tgattttatc ccccgtaaag tcgcgcaaag ctaaggaaca cgatgatggc
     2761 aacgccagtg aagaagtggt tattgcgcgt tgccccctgg tttttaccgg tgggcatcgt
     2821 ggcggtgtgg caactggcct cctcggttgg ctggctgtcg acgcgtattt tgccttcacc
     2881 ggaaggagtg gtgacggcgt tctggacgct ctccgccagc agcgaactgt gg
//
LOCUS       DAJTSA010000219         2899 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000219 DAJTSA010000000
VERSION     DAJTSA010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.221"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..646)
                     /gene="yidZ"
                     /locus_tag="OO369_004165"
     CDS             complement(<1..646)
                     /gene="yidZ"
                     /locus_tag="OO369_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR5984200.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
     gene            complement(621..1796)
                     /gene="mdtL"
                     /locus_tag="OO369_004166"
     CDS             complement(621..1796)
                     /gene="mdtL"
                     /locus_tag="OO369_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR5984201.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            complement(1928..2899)
                     /gene="tnaB"
                     /locus_tag="OO369_004167"
                     /pseudo
     CDS             complement(1928..2899)
                     /gene="tnaB"
                     /locus_tag="OO369_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
BASE COUNT          848 a          745 c          736 g          570 t
ORIGIN      
        1 aggtgtcgct ctgttcccag caaatgctga tatgcggata acgtaaaaag gtgtccagat
       61 tccacgtttc atgcagtgcc ggatgatctt tgcgtaacca gacgcagggt acatcactaa
      121 acagcacttc ataatcaatg gctaacggta gcgagcttaa cagctcccgc gagcgaggat
      181 ggctttcgcg accggaaaaa ccaatatcca cttcaccacg agtaatggca tctaaggaat
      241 cgtaatccca gttacgtagt ttgatggtcg cctgcgggta acgttggtag atccgtttcg
      301 acagcgcatt aagcatgatc atcatcagcg gcgattccgc cgccaactca aacttcaggc
      361 cgcgcggtgt ctggtggtgc ggtttatcca gcagcagatt gctcatttgc atccattccg
      421 ccagattttg ctccatgctg accattagct gtgtgggcga cagccccagc ggtgagttca
      481 caaagagcgg gtcgtcaaac cacgctctta actttgccag cgacttactc accgctgaag
      541 gggtcacgtt catccgcttc gccgctttgg tcacgctgcg ctcctgcatc agcagttgca
      601 gacagagcag cagattgaga tcaagcgtgg tgatggattt cttcatgagc ggcaacgggg
      661 cgtccaggcg cgacgaacat aatcagcaac aggctcacta tgctacaggc aatcagaatc
      721 ccgatcagca tattccatgc gccgatacca accaccgcgg ccagccaaat ccacagtgac
      781 gaaccgcaaa cctgcgcaat acctaaggtc gagctggcta cgcccgcgcg taatgaaaat
      841 ggtcctaacg cctgactcat cgctacgcca aaacctaccg agaaaccggc gcaaatcagc
      901 gtgataccaa acagagaaac cgcatgggaa ggtgaaacgg caagggtgat ccccgccgca
      961 aggaataaca cctgcgaggt gatcatcaac gtacgtggct taaaaattcc cagcgcaaat
     1021 ggcgtcgaga atgaaaaggt catgctgacg ccagcggtca gcgccataat ggtggcgtat
     1081 tctccgcgct caaaccccat gatttccatc agcaataccg gcgatgtatt gacgaaagtg
     1141 aggatcaccg aaacgctgag ggtggtgata acaacacggc tgaggaaaaa acgattaagc
     1201 agcgactcgc tattttctcg tggtttgtcc gaagcagcgg gggacgctgg gcgcgtttct
     1261 tttaaaataa acaaagacaa catcagtacc gcgatgccca tcattgccat cgcccagaac
     1321 agactctgcc acgggaattt aagcataatc agatgtccga gcactggcgc taacaccgga
     1381 atgatgcagg taataccgtt gagtaatgac agcactttag cccgacgtcg atcatccagc
     1441 gtgtcgcgca aaatggcgaa cgccaccacg taacaacagc ctgcgcccaa cccctgtaga
     1501 aatcggcctg caagaaataa cgtgctggtt tcagccagtg aacagaacac cgaggcaata
     1561 ataaatagcg ccgcgccggg tatggcgacc ggctttctcc ctgaacgatc ggccacttta
     1621 ccggcaaata gcatcgcagc tgccatcccc gccagatata cggagaacgc aatatgcaac
     1681 tgcgcttcgc tggcattgag atcggcggcg atgcgcggta aaccaacgag gtacatatca
     1741 atcccggcgg gataaagtaa aaccagggca aaactacaaa tcaaaaagcg ggacatgggc
     1801 taaagcgtcc tgatagtaag gtgacgcaaa gcatacgtgg tgaaggagga taaggcgagt
     1861 tgccatttgg acataagtga tttcctgaga ggcaagaagc cagcgaatgg ctggcttctt
     1921 gaaggattta gccaaattta ggtaacacgt taaagacgtt gccgaaccag cacaaaatca
     1981 cggttatacc aaagagaatg acaatcgccg gaataagatt gccgccccag accgtgaaca
     2041 tctgattggg aaacttcttg cgagctttga ttgcaagcac tgcgggaata atgaccgccc
     2101 agatggtggc gcacagcccg gcaccgccga tcccgtaaat aaagccgttc gggaagatca
     2161 gatacaacaa cgcaggtggc aggaaggtta acagcacggt tttgaaacgc ccgccgtggg
     2221 agttatcaat cttaaacagg tccgccagat aatcgaacaa ccccagcgtg acaccaaaga
     2281 acgaactggc aacagctaag ttagagaaca ccagcaggca aaactcgata atgccgtgct
     2341 gtttggtgcc gaggaacgat ttcaccagcg aatcaatgtt gccgcctgag gagattatcg
     2401 ccttaaagct ttcgcgcgga atattcccca tcgtgcaata gagccagaag agataaatca
     2461 ccagcgccag cagcgaacca aataccacgc ttttgattaa cttatctttg cgtttgccat
     2521 agcaaataat caggctggga atattgccgt ggaaaccaaa tgacgccaga cacaccggca
     2581 aagccataaa gatatacggg aagtaagacg ttcccgcagt ggtgctggtg gcatcgcgca
     2641 ggatggagta atcgacctgg aagaagaaag aaccaaacac gatcacaaag gagataatct
     2701 tcagcccgag gaacaatgag gtaatgcgac tggcggctaa cgaacttatc cacaatacgt
     2761 ggattagcgt gatagcccaa attcattgat atcgtttcac taatgatcgc accattagca
     2821 gagatatagg cataagtgag gatatagaga acgaaggcaa cggtaatacc gctgataatg
     2881 ttccaggtgt tacctatta
//
LOCUS       DAJTSA010000220         2897 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000220 DAJTSA010000000
VERSION     DAJTSA010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.222"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            550..693
                     /locus_tag="OO369_004168"
     CDS             550..693
                     /locus_tag="OO369_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR5984202.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            706..915
                     /gene="cspE"
                     /locus_tag="OO369_004169"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             706..915
                     /gene="cspE"
                     /locus_tag="OO369_004169"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5984203.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            1081..1890
                     /gene="rlmA"
                     /locus_tag="OO369_004170"
     CDS             1081..1890
                     /gene="rlmA"
                     /locus_tag="OO369_004170"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR5984204.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1887..2453)
                     /gene="mntP"
                     /locus_tag="OO369_004171"
     CDS             complement(1887..2453)
                     /gene="mntP"
                     /locus_tag="OO369_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR5984205.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          794 a          646 c          667 g          790 t
ORIGIN      
        1 atattattta tttgcgtaac gggatatcag aaaaagatat tttcctcatt aatttattag
       61 gatgtttaca tcagatttgt gattaagcgt ggtattattt attagcgaaa cgtttctctc
      121 ttgatttttt tgctcactca tcaatttttc ttattttaaa tttacaatcc ctttggggat
      181 tgacttctct ttagggtaat tgatagccgt taactgactg ttttatgaga aaaagtgata
      241 taacttttta ttcattgcat agcagaaaat gtgatattgc acgcactatg taataacttc
      301 tccctctggc ctggaacaac tgaacttatt gaactatgtt agaaactacg ccagtttaag
      361 tatctgcctg aactggcaag gttaagcaca atgatatatc ggcgcgtatt ccgttgcata
      421 agtgtgcaaa aaaagtggaa gacgtatcga gatttgtgcg tctgatcgag atatgtttaa
      481 aaatggcttg ccataattaa cgttgtatgt gataaaagat ttcgggttaa acgaggtaca
      541 gttctgttta tgtgtggcat tttcagtaaa gaagtcctga gtaaacacgt tgacgttgaa
      601 taccgcttct ctgccgagcc ttatattggt gcctcatgca gtaatgtgtc agttttatct
      661 atgttatgcc tgcgggcgaa gaaaacaatc taaggaattt ttcaaatggc aaagattaaa
      721 ggtcaggtta agtggttcaa cgagtctaaa ggttttggct tcattactcc ggctgatggc
      781 agcaaagatg tgttcgtaca cttctccgct atccagggta atggcttcaa aactctggct
      841 gaaggtcaga acgttgagtt cgaaattcag gacggccaga aaggtccggc agctgttaac
      901 gtaacagcta tctgatcgaa tccactgatc tgaagtgtga atacgcttca atctcgctat
      961 aaagcctcgt cgaatgcgag gctttttact atgctttatc ttcgctcctg gcgttcggat
     1021 atttgcccgc cgcgtgattc gcgttacact tgcggccttt agtatcctgc cggagttgtc
     1081 atgtcgtttt cctgtccact ttgccatcag cctctttcgc gtgaaaaaaa cagctatatc
     1141 tgtccccaac gacatcagtt tgatatggcg aaagaagggt atgtcaatct gctgcccgtt
     1201 cagcataaac ggtctcgtga tccgggcgac agcgcggaaa tgatgcaagc acgccgcgca
     1261 ttcttagatg ccggacatta tcagccgctg cgtgatgcaa ttgtcgccca actgcgggaa
     1321 cggcttgatg aaaaggccac ggcggtgctg gatattggct gtggtgaagg gtattacaca
     1381 cacgcatttg ccgatgcgtt gcccgaaatc accacgtttg gcctggatgt ctcgaaggta
     1441 gcgataaaag cggcggcgaa acgctatccg caggtcactc tttgtgtcgc ttccagccac
     1501 cgtttgccgt tttccgatac cagtatggac gccataatac gtatttacgc gccgtgtaaa
     1561 gcagaagaat tagcacgagt agtgaagccc ggcggctggg tcattactgc cacgccggga
     1621 ccgcgacatt tgatggagct gaaggggctg atttacaatg aagtacatct tcatgcacct
     1681 catgcagaac aactggaagg ttttacatta cagcagagtg cggagttgtg ttatccgatg
     1741 cgtcttcgcg gtgatgaagc cgtcgcatta ttgcagatga cgccgtttgc ctggcgtgcg
     1801 aagccagaag tctggcaaac actggcagca aaagaagtgt tcgactgcca gacggacttt
     1861 aatattcacc tctggcagcg ttcttattaa ccgtggaagt gcgtccagag gatctggacg
     1921 ccgatgccga tcagcaccag cccgccgaga atttccgctt ttttcccaat aattgagccg
     1981 ataaagcgac caaccatcat ccctaatgtc gacataatca aggttgcaca accaatggcc
     2041 aatgcggtcg cgataatgtt gacctgaagg aaagcaagac caacacccac agccatggca
     2101 tccaggctgg tggcaatcgc ggtggttacc agtagccaga aaccgtgccg acggcgcggc
     2161 tcttcatctt catcatctgc gccacgaaaa ccctcaataa tcattcgccc gccgaggaat
     2221 atcagcagca caaacgcaat ccagtgattc cattcaagga caaaccggct ggctaacatg
     2281 cccattcccc agccgatcag cggcgtcagg gtttcgacgg caccaaaaat aaggccggtt
     2341 cgcaatgctt cagaaaattt cggtttatgg aggggggcac ctttaccgat tgatgcagca
     2401 aatgcatcca tcgacatacc aaacgcaaga agaacagtag cagtgatatt catgacaacg
     2461 tcctgaccgg ggtatccata aaacacatca tcgcccccag caaacatgca tacatgaaca
     2521 acaagccgcg atgggttttc gcgttcatgc aggcatagcg attgatgtgc atatggtctc
     2581 gcctgactga aagtcttcag tccgtacgtg ccacgttttg caacgagtat gttgacacgt
     2641 acatttccgg aatctggaaa tcggctactc cccaaatgag ggcgcaacct taacatattt
     2701 tgagaatata aaacaactgt tggaatatat tatttcattg ctttattgat aacgattttc
     2761 atttatgttt atatatcaat gaaacgttaa aataaataac aaataaggga agagataata
     2821 tagcctaagc tatgtctgat tattaaaaat acactaaaca tagctttggc taaattcata
     2881 agttattgag ttgatac
//
LOCUS       DAJTSA010000221         2894 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000221 DAJTSA010000000
VERSION     DAJTSA010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.223"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..308)
                     /locus_tag="OO369_004172"
     CDS             complement(<1..308)
                     /locus_tag="OO369_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR5984206.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     "
     gene            complement(355..1074)
                     /gene="yehT"
                     /locus_tag="OO369_004173"
     CDS             complement(355..1074)
                     /gene="yehT"
                     /locus_tag="OO369_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR5984207.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1071..2756)
                     /gene="btsS"
                     /locus_tag="OO369_004174"
     CDS             complement(1071..2756)
                     /gene="btsS"
                     /locus_tag="OO369_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR5984208.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          748 a          774 c          657 g          715 t
ORIGIN      
        1 tcatcggttt tcagcgaaaa cgcgatgcgt aattttttca gcacgatgtt gttattaatg
       61 cgacgttccg gctccagtgc cggagcagac tcatccttgc cgcgtttttc ataaatcagg
      121 ccattgagga atgacgacaa aacaatgtcc ggacaacgct gaaaaccctc ttcgtcttct
      181 ttacgtagcc agacggcgat ctgttccgcg gtggcttcga cattatccag cgccagaata
      241 cgcaccaggt cattattatt ggctttcaaa atgtagcgca cgctgcgcag aatatcgtta
      301 cttatcatga ggccttcagg tgttgatgag gcaaaaagcc attttagcag tcttttacag
      361 gccaatcgcc tcttttaagc ttttcagata acggcggctg accggcacgg ttaagccatt
      421 acgcaaaatc aactcggcct ggccgttatc ttccagacga atctcctgta aatgcgcgag
      481 gttaaccaga tactgacgat ggcagcgcag tagtggtgta cgactttcca aggtacgtaa
      541 tgtcaattcg gtaaagccct ctttcccttc gtggctggta acgtagacac cgctcatccg
      601 actgctgaca aatgccacat ctttcatttg cagcaaataa atccgactat gccccgtaca
      661 agggataaat ttcagcgcct gttgattttc cggtaacagc gaaacatcct gcttgctgcg
      721 ctcctgacgc aatcgcgcca gcgttttctc cagtcgcgct tcatcaattg gcttcagcag
      781 ataatcaaag gcatgttctt caaaggcttt aatagcgtat tcgtcaaacg cagtgagaaa
      841 aacaatatac gggcgatgtt ccgggtcaag catccccacc atttccagac cactgatgcg
      901 cggcatctgg atatcgagaa acagcacatc cgggcgcagt ttatgcaccg cgccgatccc
      961 ttccacggcg tttgaacact ctccaacgat ttcaatatcg ctctgctcct gcaaaaatat
     1021 acgcaggttc tcccgtgcta acggttcatc atcgacaatt aagactttaa tcatgcctcg
     1081 tccctccatg gtagtcgtaa cgttattcgg gtgtaactat caggctcaca ggcgacgctt
     1141 attccatagt catcgccaaa ccgttcacgt aaacgcttat ccaccagatt catccccagc
     1201 ccactggcat tggttaccgg ttgatacaaa ccggcattgt cttcgatctc cagcatcaaa
     1261 tgttgcccct cacgtcgggc gctgattgcc actcgccctg tatccagcag ttgtgatgtc
     1321 ccatgtttaa tggcgttttc cactatcggt tgcagggtaa acgcgggcaa ttgctgctgg
     1381 gataattctt gcggaatagc aatgttgacc tgcaaccgcg actggaagcg cgccttttca
     1441 atttgcagat aagcgttcac atgttcaatt tcgtcggcga gagtaacaaa ctccgaaggc
     1501 cgctttaagt ttttgcggaa aaaagtggaa agatactgca ccagctggct ggcctgttcg
     1561 ctgtcgcggc ggatcaccgc tttaatggtg ttaagcgcat taaacaaaaa atgaggattc
     1621 acctgggcgt gaagcagttt gatttctgac tgggtgagca tcgctttttg ccgctcatat
     1681 tgccctgcaa gaatctgcgc cgaaagcagt tgcgcaatcc cctcgcccag cgtgcggttt
     1741 attgaactga ataaccggtt tttggcttca tacaatttga tggtgcccat cacgcgctga
     1801 ttttcgccac gcaacggaat taccagcgtc gaccccagtt tgcattgcgg atgcaaagag
     1861 caacgataag gtacttcgtt gccatcagcg tagaccactt caccggtttc aatcgctttt
     1921 aaggtgtaag tcgaagaaat cggtttgccg ggtaaatggt ggtcgtcacc aattccggta
     1981 aaggccagca atttctctcg atcggtaatc gcgactgcac caatatccag ctcctgatac
     2041 agtacctgag ccactttcat gctgttcact tcgttaaacc cctgtcgcaa aatgccttcc
     2101 gtcgaggctg ccactttcag cgcagtggca gaaaaagccg aagtgtattt ttcaaacatc
     2161 gcgcgtttat cgagcaatat acgcataaac agcgccgcgc cgacggtatt ggtgaccatc
     2221 attggcgcag caatattact caccagacgc accgcatctt cataaggtcg ggcgatcgca
     2281 aggatgatca gcatttgcac catttcagcg acgaacgtga cggcaccggc ggtaatgggg
     2341 ttaaagactt tatcagtgcg cccgcgacgg atcaggatgc tgtgtaccag gccgccgagc
     2401 aatccttcaa cgatggttga gatcatgcaa cttagcgcgg tcatgccccc catcgaatat
     2461 cgatgtaagc cgccggtcag accaaccagc ccaccgacga ccggaccgcc gagtaagccg
     2521 cccattaccg cgcctatcgc acgggtattg gcaatagaat cgtcaatgtg caacccaaac
     2581 caggtgccca tgatgcagaa gatggaaaag acgatgtagc agagaaattt atgcggcaga
     2641 cgaaccgtga cctgcattaa cggtatgaat aatggcgttt tactcattaa ccacgcaatg
     2701 actaaaaaaa cgcacatctg ctgaagcagc agcaacacca gattaaaatc gtacataccc
     2761 gcaaaccaca cttcccttta aaacgcgtaa catacattgc ctgcgtttaa ctttctttga
     2821 actcttgcag aaaaatgaga attcgtgagt acgatcactc aaaatcgcct ggcaaaaata
     2881 aaatcaccct atag
//
LOCUS       DAJTSA010000222         2889 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000222 DAJTSA010000000
VERSION     DAJTSA010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.224"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..779
                     /gene="lsrF"
                     /locus_tag="OO369_004175"
     CDS             <1..779
                     /gene="lsrF"
                     /locus_tag="OO369_004175"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCR5984209.1"
                     /translation="WGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINIAP
                     LFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNS
                     CAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRI
                     AAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGVD
                     MGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            803..1093
                     /gene="lsrG"
                     /locus_tag="OO369_004176"
     CDS             803..1093
                     /gene="lsrG"
                     /locus_tag="OO369_004176"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR5984210.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1133..1891
                     /gene="tam"
                     /locus_tag="OO369_004177"
     CDS             1133..1891
                     /gene="tam"
                     /locus_tag="OO369_004177"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR5984211.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1895..2809)
                     /locus_tag="OO369_004178"
     CDS             complement(1895..2809)
                     /locus_tag="OO369_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCR5984212.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          760 a          667 c          771 g          691 t
ORIGIN      
        1 attggggaat gcagtcacgc ttatcgtgga tatttaatcc gaaaacgggt aaaactgtga
       61 tgctggcctt tgaccatggt tattttcagg ggccgactat cggacttgaa cgcattgata
      121 taaatatcgc cccgctgttt gaacatgccg atgtattaat gtgtacgcgc ggcattttgc
      181 gcagcgtagt tcccccggcg accaataagc cggtggtact gcgggcgtca ggtgcgaact
      241 ctattctggc ggaattaagt aatgaagccg tggcgttatc gatggatgat gccgtgcgcc
      301 tgaacagttg cgcggtggcg gcgcaggttt atatcggcag cgaatatgaa catcagtcga
      361 tcaaaaatat tattcagctg gtcgatgccg gaatgaaagt gggaatgccg accatggccg
      421 tgactggcgt gggcaaagat atggtgcgcg atcagcgtta tttctcgctc gcgactcgaa
      481 tcgccgctga aatgggggcg caaattatca aaacctatta tgtcgaaaaa ggttttgaac
      541 ggattgttgc cggatgtccg gtacccattg ttattgctgg cggtaaaaaa ttaccggagc
      601 gcgagacgct ggaaatgtgc tggcaggcta tcgatcaggg cgcttctggt gtggatatgg
      661 ggcgtaatat tttccagtct gaccatccgg tggcgatgat gaaagccgta caggcggtgg
      721 ttcaccataa cgaaacggct gatcgggcat atgaactcta tctgagtgaa aaacagtaac
      781 tgcggattta aggagaagaa ttatgcacgt cacactggtt gaaattaacg ttcatgaaga
      841 caaggttgac gagtttatcg aagtttttcg ccagaaccac ctgggctctg tacaggaaga
      901 aggtaatttg cgcttcgatg tcttacagga cccggaagtg aattcgcgct tttatattta
      961 cgaagcctat aaagatgaag acacagtggc attccataaa accacgcctc actacaaaac
     1021 ctgtgtcgcg aaactggaat ctttaatgac cgggccgcgt aaaaaacgtc tgttcaatgg
     1081 gttgatgccg tgaggctaca attggggtaa cgcgctgacg ggagtaaaaa aaatgtctga
     1141 ctggaacccc tctttatatc tgcacttttc cgctgaacga tcgcgtccgg cggtggagct
     1201 gcttgccaga gtgccgctgg agaatgtcga atatgttgcc gatcttggct gtggcccagg
     1261 taacagcacc gccctgctgc atcaacgttg gcctgcggcc aggataacag gcatcgactc
     1321 gtctccggcg atgattgctg aagcgcgcag tgctttgcca gactgccagt tcgtggaagc
     1381 agacatccgc aactggcaac cggaacaggc actcgatctg atttttgcta atgcctcact
     1441 gcaatggctg cccgaccact acgaattgtt tcctcacttg gtttctttac ttaatccgca
     1501 gggcgtgctg gcagtacaga tgccagataa ctggctggag ccgacccatg tgctcatgcg
     1561 cgaagttgct tgggaacaaa actacccgga tcgcgggcgt gagccgttgg ctggcgttca
     1621 tgcttactac gatattttga gcgaagccgg atgtgaggtc gatatctggc gaaccactta
     1681 ctatcaccag atgccgtcgc accaggcgat tatcgattgg gtgaccgcca ctggattacg
     1741 tccgtggtta caggatctga ccgagagcga acagcagctt tttcttacgc gctaccatca
     1801 gatgctggaa gagcagtatc cactgcaaga gaacggacag atactgctgg catttccgcg
     1861 tctgtttatt gttgcccgcc gtacggagta attatcacgt cagctggtaa tgacgatcgg
     1921 gaagaatttt tgctggaatt tctgcttcat cgttcatctg taacaggtca atttcaatag
     1981 cgttgcagat ggcatccagc ggtaaatcat tgttttcagt accgaacgga tcttccagtt
     2041 cttccgccag acaatccagc gaaataaaag tgtaggaaat cagcacagag ataaaaggcg
     2101 tcatgtaatg caggtccacg accagcgcga acggcagcat gatacaaaac agataaacgg
     2161 tacgatgcaa aatcagggtg taggcaaagg gaattggcgt gtaggcaatg cgctcacagc
     2221 ctgccagaac cgctgaaata tcattaagcc gatcgttgag gctaataaac aggatatctg
     2281 aaagctgtcc attgcggcgc tgaaccgcca accattctcc cattattaac aagatacggt
     2341 tagctggaga gttcgaagcc agtacacgct gaagatctcc agtcttgaga taatgagcca
     2401 gcacttccgc ctgtggctgt ttgcgtaatg tcatgcgtaa acagtgggcg aaggcgattt
     2461 gcagcctggc aaactccctt acacttgccg aatccggcaa tgtcgttttt acctctcgca
     2521 gtaacgaccg tgaggcaatc atcaactgcc cccaaagttt tcgcgcttca acgtaacggg
     2581 cgtacccggc attattacga aaaccaagaa aaatggcgat ggcgacaccg agaatgctga
     2641 acggtgcgag ggtgaatttg atgcccagat gcgtgtacca gggcagcatg aatataacag
     2701 caatagaaaa gagaaaattg agtagtaagc gcgaggatat cttggataat actgagccgt
     2761 gccagacaaa aatacggcgc agccagtgtt gttgtggacg aacaatcatg gttatcttca
     2821 ggcgtggaaa agtcgcccta ttaaacgtga ttacgatcac attctcaaga cgctacttac
     2881 aaattacct
//
LOCUS       DAJTSA010000223         2810 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000223 DAJTSA010000000
VERSION     DAJTSA010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.225"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="OO369_004179"
     CDS             complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="OO369_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR5984213.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1096..1275
                     /locus_tag="OO369_004180"
                     /pseudo
     CDS             1096..1275
                     /locus_tag="OO369_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1292..1982)
                     /locus_tag="OO369_004181"
                     /pseudo
     CDS             complement(1292..1982)
                     /locus_tag="OO369_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1905..2156
                     /locus_tag="OO369_004182"
     CDS             1905..2156
                     /locus_tag="OO369_004182"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984214.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAII"
     gene            complement(2176..>2810)
                     /locus_tag="OO369_004183"
     CDS             complement(2176..>2810)
                     /locus_tag="OO369_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR5984215.1"
                     /translation="RWLPSVGIVWRRAAPTLRIRDPHKDEKMAAIHKALDECSAEHPV
                     FYEDEVDIHLHPKIGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVSYVGGNSKS
                     SALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWV
                     NHVERLWQALHDTITRNHQCRSMWQLLKKVRHFMETVSPFPGGKHGLAKV"
BASE COUNT          761 a          583 c          651 g          815 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaaaacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt aagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aagaaactga tgagtgtgta ttatttacag agaacataat gctaggtcaa
      781 ccttatattt gtgagaataa atatttaaaa ttgtatattt atacaaattt cacttaccat
      841 ttccgatatt aagttttaat agtttccatt ttacacataa cagaagttag ttgtaattta
      901 tttatggagg ttttctatgg aattcttttt ttattgtggc tgcagtcaat aataacactg
      961 aattcaacat tccagtgagc aaggaaatgt aagccgagta agtatatttg ttttacatca
     1021 tattttagtc aagctcattt attcctatta tagaatgaca aattaaaatt actgtaaaaa
     1081 aacgatacac atttaatacg agggaggccg gcagaaaaac ggcttgcggt caggcaggag
     1141 aaatgtgtgc cgcgaatgaa gtccttgtat gactggataa agctccagag gaaaactttg
     1201 tcaaaccatg ctgaaatggt aaaggcgttc aactatatcc tgaatcactg gaatgcactg
     1261 aatgagtttt gccgggtagt acttccaatc attagaatat gtgcttttca atgaaggaac
     1321 cgatgacttt ttcgtggatt tccacggaac gcgagaagca gattgtttta cgtgccagcc
     1381 gcttgatgcg ggggcggagg gtcaggttgt tacgttcata cgttgcgtga agattttgcc
     1441 ggtcaggtgt ttctcctccg gcacttctct ggcgtagctg ccccagtcat cgctggtcac
     1501 catgccgata cagaacggag tcaacagggc taacagttcc cgacaggtct catcattccg
     1561 tggcccgcaa gtgtaagcca acacaccgcc agttttagtg ttgtacgcat accagggcca
     1621 gtgttgttgc gccttgctgc caacgaagcg ccactgctca tcaagttcac agataagggc
     1681 aacatcagcg tgagcgactg gcgcagatgt tatccgccgg ggcgagagct ttttaatgtc
     1741 cgtatgacgg tattgatgcc gattttcagt gtcctggcgg tatcacggcc ccctgcgccg
     1801 ttatgtgcca tttcaacgat ctgctcctta acgccgagtt ttctggcttc ataagagtaa
     1861 gtgagctgga aaacacgttt acatgagcga caacggaaac gctcatgccg ggaacaacta
     1921 cggccatgtc gataaacttc atcggaatta cagcgcggac aatgaacagt aacggtagtc
     1981 atggaaaacc tcaaatacca gtagattacc agaaacttct atcgattgaa gacattacct
     2041 tttgccgcaa taggtggggt aacattggtg aaaacgcatt acgccgggtg gcagttggaa
     2101 aaaaattatc ttttttcggc tctgatcgtg gtggagaaaa tgcggcaata atctaaatag
     2161 ctgcgcttaa taccgctaca cttttgccag cccatgtttg cctccgggga atgggctgac
     2221 ggtttccata aaatggcgaa cttttttcaa cagttgccac attgagcggc actgatgatt
     2281 acgcgttatt gtgtcgtgaa gtgcctgcca tagccgttca acatgattca cccatggcga
     2341 gtaaaccggc tgataaatgc ccctgaactt cgggttctcc ttcagccagc tctgtgtttc
     2401 ccggcttttg tggataatgt agttgtccac gatcagcgtg atggttttcg cacgacggta
     2461 tgtcgcttta agccgcttca gcaggctgat gaacagcgcc gaacttttgc tgttgccgcc
     2521 cacatagctg actttacctg tcccgctgtg cagcgctccg gccagataat atttttcatt
     2581 ctgtcccggc gtgaccaccc gtttttgctg tccgcgcagt tgccagtccg caccgatttt
     2641 gggatgaaga tggatatcca cttcatcttc ataaaagacc ggatgctctg cgctgcattc
     2701 gtccagtgct ttatggattg ctgccatctt ttcatcttta tgcgggtcac ggatacgcag
     2761 agttggcgca gcccttcgcc acacaatccc cacagacggc aaccagcggc
//
LOCUS       DAJTSA010000224         2713 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000224 DAJTSA010000000
VERSION     DAJTSA010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.226"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            52..2574
                     /locus_tag="OO369_004184"
     CDS             52..2574
                     /locus_tag="OO369_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5984216.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSFSTPESESGLIEQGVTCH"
     gene            2585..>2713
                     /locus_tag="OO369_004185"
     CDS             2585..>2713
                     /locus_tag="OO369_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5984217.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFS"
BASE COUNT          727 a          584 c          678 g          724 t
ORIGIN      
        1 agtgacccga taaaaatgac gctgtaagca agggataagg ataggcgtat tatgatgacg
       61 accagaatag tggttggcct cacggcaggg acgtgtctga ttttctcgca aaacctgatg
      121 gccgaggtca gtgtattcaa tccggcgctt ctggaaatca accatcaatc cggagtcgat
      181 attcgccagt ttaatcgggc aaacctgatg cccccaggtg tttatagcgt tgatattttt
      241 atcaacggta aaatgtttga acgtcaggat gtgacatttg ttcaggataa tccagatgct
      301 gatctgcacg cttgctttat tgccattaaa aaaacactgt cctcctttgg cataaaagtt
      361 gatgcgctca aatcgttcaa tgatgtggat gagacggttt gcctcgatcc tgctccacgt
      421 attgaaggct catcctggca gtttgacagt gataaattgc agctgaatat atccattcac
      481 caaatctaca tggacgcgat ggcttatgat tacatcagcc ccacgcgttg ggatgagggg
      541 attaatgcgc tcaccatcaa ctacgatttt tctggttcac atacactacg ttcagattat
      601 ggttcacaag agacagatac cagttatctc aatctgcgca atggactgaa tattggaccg
      661 tggcggctac gtaattacag tactttaaac accagcgatg gccgtgcgga atacaactcc
      721 attagtacct ggatacagcg cgatattgcc gcgttaagaa gccagattat gattggtgat
      781 acgtggacgg cgagcgatat tttcgacagt acgcaaattc gcggcgcgcg tttgtatact
      841 gataacgata tgctacccgc cagccagaat ggctttgctc ctgtggttcg tgggattgca
      901 aagtccaacg ccaccgtcat cattcggcag aatggctacg tgatttatca gtcagccgtt
      961 ccacaaggtg cttttgagat caccgatctc aacaccgcaa gtacaggtgg cgatttggac
     1021 gtaaccatca aagaagaaga cggtagcgaa caacgattca cccaacctta tgcttcattg
     1081 gcgattctta aacgtgaagg tctgacagat gttgatgtca gcgtgggtga attgcgcgat
     1141 gaagacggat ttacaccgga cgtccttcag gcgcaaatac ttcatggttt ttcccacggg
     1201 atcactttat atggaggtat gcaggctgct gaaaattatg gttctgcagc tctgggtgtc
     1261 ggtaaagatc ttggcgcttt gggcgcaatt tctttcgatg tgacacatgc tcgtgcgaat
     1321 tttagccatg atgatacaga aacgggtcag tcatatcgct ttctctattc aaaactattt
     1381 gacgacacag acactagctt gcgcctggtt ggctatcgtt actccaccga gggctactat
     1441 accctcaatg agtgggcatc gcggcgcaac agccctgaag acttttggga aacaggtaac
     1501 cgacgtagtc gcgtggaggg aacgctaacg cagtcgttgg ggagagatta tggcaattta
     1561 tacctgacat taagccggca acaatactgg cataccgatg atgtcgaacg attaatgcaa
     1621 tttggctaca gcagtagctg gaagcgtctc tcgtggaacg tctcctggag ttattccaat
     1681 actgccagac aggggacggg gaacaaccat gccagtgata acaccagtga gcagatctac
     1741 atgctctctt tatctgttcc tttatcgggc tggtggggta atagttacgc cacctattct
     1801 gtttcgcaaa acgataattc cggtagctca catcaactcg gactcagcgg tacggcgctg
     1861 gaaagaaata acctttcatg gaatttaatg cagtcctata acagtcatga tgatgaggtt
     1921 ggcggtaata tgtccctgac ctatgatggc tcttatggca cggtgaacgg cagctataac
     1981 tatagccaaa attcccagag gctgaattat ggtatcagag ggggaattct ggcacacagc
     2041 gaaggggtaa cgttaagtca ggagttaggt gaaactattg ctcttgttaa agcacctggg
     2101 gccgccaggt tagaaataga taatatgcgc ggtgctgcga cggactggcg cggctatacg
     2161 gtcaagacac agctaaaccc ttatgatgaa aatcgggtag caatcagcga taactatttc
     2221 tcgaagtcga atatagaact tgataatacc gtcgttacga tggttcccac gcgtggtgca
     2281 gtggttaaag cggagtttgt gactcatgtg ggttatcgcg ttctcttcag agtgttaaat
     2341 gcaaatggta aaccggtacc ttttggagcc attgctgcga tacaagatgc aagtttggca
     2401 gattcaggaa ttgtcggtga ccgtggcgaa ctttatcttt ctggtctacc agaaaaagga
     2461 caggttacgt tatcctgggg agaaaacgcc tcaacaaaat gcatcttcaa ttattcattt
     2521 tcgacaccag aaagtgagag cggattaatt gaacagggtg tgacatgtca ttaaaaggat
     2581 ttttatgaac aagtatatca aacagtggtg ctttgctgtg tttatgctct cgttaagtag
     2641 tgtagcactt gcagctccta aaggtatctg tacccctgat aatggcgtat ttcacagtac
     2701 gcttgatttc tcg
//
LOCUS       DAJTSA010000225         2710 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000225 DAJTSA010000000
VERSION     DAJTSA010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.227"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            6..224
                     /locus_tag="OO369_004186"
     CDS             6..224
                     /locus_tag="OO369_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR5984218.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="OO369_004187"
     CDS             226..591
                     /locus_tag="OO369_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5984219.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            588..1253
                     /locus_tag="OO369_004188"
     CDS             588..1253
                     /locus_tag="OO369_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984220.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..1614
                     /locus_tag="OO369_004189"
                     /pseudo
     CDS             1253..1614
                     /locus_tag="OO369_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            2069..2224
                     /gene="hokD"
                     /locus_tag="OO369_004190"
     CDS             2069..2224
                     /gene="hokD"
                     /locus_tag="OO369_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5984221.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
BASE COUNT          770 a          556 c          695 g          689 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcgggaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaatatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga tgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggataagc tgtagtgatg cagttcctgc tgaatattgc
     1441 gatgtgattc ttctcgatga tctcgggaat gtattccccg gttcctggga taaggttttt
     1501 tgccccactc gtggcgggaa taagatggct tttgtggaca aagacggcgt tgaagtagag
     1561 agctcaactc actggatgcc gctaccggaa ccaccgcagg aggtgaatca atgacctggc
     1621 ctgaggcatt caccacggta ggaattgcga tggcggtggc gctggtggtg tattcgattt
     1681 gccgttgggg ttaacaaaca aaaaccccgg attgatggtc cggggttttt gaaggaaaca
     1741 aaacagaaac agcaattgcc gttacctgtt gttaccatgg caagtaaacg tatctcaggc
     1801 gagcgcattg cgccgttctg acgcagataa attagcctgg atagaaggtg ctggcaataa
     1861 aaaatagcgt tttcttatcg gtatcggtaa gattgctgcg ggtgcttgag gctgtctgcc
     1921 tcgggcatgc cactgtaagg cagacagaga aaagccccag ttaacattac gcgtcctgca
     1981 agacgcttaa cattaatctg aggcccaatc tatgtctcac aaatgtaggt tagcctctta
     2041 cgtgccgaaa ggcaaggaga agcaggctat gaagcagcaa aaggcgatgt taatcgccct
     2101 gatcgtcatc tgtttaaccg tcatagtgac ggcactggta acgaggaaag acctctgcga
     2161 ggtacgaatc cgaaccggcc agacggaggt cgctgtcttc acagcttacg aacctgagga
     2221 gtaagagacc aggcggggga gaaatccctc gccacctctc atgtgtcagg catcctcaac
     2281 gcacccgcac taaacccgct tcggcgggtt ttttgttgcg tgctgaatac gcagggtgaa
     2341 aaataaccat atatttgatt atatacacaa caaaaaataa aagtcattgt acttgcacat
     2401 taaacaatca aatatacggc gtgaaataaa tatttatcag attaatattt ttgtctctat
     2461 gtggatataa ccgtttgtac ttataaacct gggggcatcg tggaaaaaat aaagaaacta
     2521 tttagtagca aatacgcagt catacgtcgt gatgacctgt cagttatagt cgaaatggat
     2581 tacttccctg aaaccccaaa atcaataatg tatcgtaatg gtcgaaaggc aattttttta
     2641 ccgatgaggg taagtgacat tatgggaaat gataaactgc tggatgaatt gcgagtcaga
     2701 gcatcctgtt
//
LOCUS       DAJTSA010000226         2692 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000226 DAJTSA010000000
VERSION     DAJTSA010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.228"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(498..1277)
                     /gene="ipaJ"
                     /locus_tag="OO369_004191"
     CDS             complement(498..1277)
                     /gene="ipaJ"
                     /locus_tag="OO369_004191"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR5984222.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(1877..2014)
                     /locus_tag="OO369_004192"
     CDS             complement(1877..2014)
                     /locus_tag="OO369_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984223.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
BASE COUNT          821 a          655 c          414 g          802 t
ORIGIN      
        1 ttttattctc ctaatcttcc cataaaatga ccgacttaca ttattttata attataaaca
       61 agtttgattt taaaatcata ttaattatta acaagagatt tatttttcta accccaaaag
      121 ttatgcttgg tcaaagagta acttataata tccatacccc tcatagggct tacagtttaa
      181 attgcgggta taaaatacat ctattttcaa ataaaataaa gcatactcca attacaacga
      241 gtgactaaaa taaaaatatc atccaagtca atcactgttt gctctgcaaa atatgtcagc
      301 cttctcaaac ttaccagcac ttaaatattt aagcacacaa tcattccttg agggagttca
      361 cttatttcaa gagaaacatt tcgtcttccc acacaactaa tccgcccaat atagctaaat
      421 ttgtactcag taatatcatt tgttatgccc ccccacccca aaatattcaa gctccttcac
      481 agataaaaaa cgacaattta caaagcctca ttagttataa ctatggaaat acctatttta
      541 gtatatccta taaagttaga attacttctt cttgcaccta attccatagt tgaaaaacaa
      601 gaataatttt ctcctacagc tggatccata tagcttccat caggtctaca caacacccag
      661 tggaggccga caggaactcc aataaaggat acagcaacag cttccaaaac tctttggtta
      721 gaagataaca catctcgttg atgtactata ttacatccca tgccaattaa taagtccctt
      781 gcatcgggat aaataaaact aattacttgc gaaaaaatgt tactttcttc aacgacataa
      841 gcattcaatc caagaagcct cgtagccatg actatcttat caggcataga ataaccggca
      901 tccgcgatat tatctttgtg tattctcgga ttataattac cactagttat taagtataaa
      961 tcccgctcac aacgattatc caaatcaaga gaactcttcc ttgtcatttc ggacatagac
     1021 cctttgtatt gtggtatttt atcaacccct aattcctttg cagcacagag taaactggca
     1081 gctccacaag aattgtcata catctgcttc ctcgccaaaa aacaaccttg ttcgtttact
     1141 ctattctcat ttaccctcac atcagtctga tgagatatca tatattctct tggtatagcc
     1201 ccctgcaata aaaccccata taacataaca cctgtatgaa tacatccccg tttgcaaggc
     1261 ttccgttgtt ccgacatcga cgagtaattg cagcacagta ttgcatccag caatgcgaac
     1321 tactatgaga attagctgac cgggtcctta ttccccgaac gaactttaaa tccaaaacct
     1381 catactcttt tccgtcgtca tcacgtccgt caccataaca acctttcagc atccaccctt
     1441 ttattaagag ttaatgtcca tcagcatcag caattaatac cgcctgatac tgacatattt
     1501 ttgtcgtatt tacgtccata atcggttatt gtgatacagg tcacaccagc ttatttgcta
     1561 ttgttacaat aaccaccttg gttgagagct gttttttcaa ctttgttatc aactccccca
     1621 actccttggt cagtaaggct gccgaccgag cacagtgaat agatagtatc cgcaggtacc
     1681 accatgctta cttaatactc agaaggcgaa gtgctctcca attaactctg ccgcactatg
     1741 cttataagct gcttttcaat gccgtctatc aacatgtcca gttcattcaa tctgttgtac
     1801 tggtaatcca acgcagtaat gatatatgca ggtattcttt catccactcc agtaacgttt
     1861 catgcaaagc attaatttac gcgctacgga acttcaccag ttgactgagc aaacgatgtg
     1921 ggaccagtac tgcctgcagt tcttctattg ttacagcgat ttctttacca aacagctgaa
     1981 ccaccatcaa gataaagata gcctgaacat tcatcgcacc attttgttac caatggcaaa
     2041 agatttaacg aaccgtgcct gcatcaggcg gactttttat ccctgctttg ttagctccct
     2101 aaccttgtac agagagcctc cataagctcc catatcaagc agacatggtt cgcagttact
     2161 gacatacacc agaaaactct gacagcagac tcacttatcc tccacttcac tcatatcctt
     2221 attggtgaga tgaacctgaa tgacatattt tgcgacataa acgccaacca gtgtatattt
     2281 cacctgtaga gattctacaa tctgtagcgt tatgcatcta atgttaggct ctatgatgtc
     2341 agaaatctgc gagactctac accttctgca tttaccttta tttatcacca tgctcctcag
     2401 ttaacggaga acgtgttcat tgcattttgt cacttacatt tcgcctttgg tatcgggggg
     2461 atataggttc caacgttccg catgagaacc tgaaccggct tcacgccacc ttgataccag
     2521 atgccgcaca ggtagtcaac aagtgtcacg aacggtgcaa tagtgatcca cacccaacgc
     2581 ctgaaatcag atccaggggg taatctgctc tcctgattca ggagagctta tggtcacttt
     2641 tgagacagtt atggaaatta aaatcctgca caagcaggga atgagtagcc gg
//
LOCUS       DAJTSA010000227         2627 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000227 DAJTSA010000000
VERSION     DAJTSA010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.229"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            624..1223
                     /locus_tag="OO369_004193"
     CDS             624..1223
                     /locus_tag="OO369_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5984224.1"
                     /translation="MQTYNTNPAYEMAPQLLEHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEMLQLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTEGNGYYHWCRFNKNYPVNINHVIHYSDHKAVNDLRYAISYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLDSPLPGICALV"
     gene            1596..1979
                     /locus_tag="OO369_004194"
     CDS             1596..1979
                     /locus_tag="OO369_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984225.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
     gene            1976..2401
                     /locus_tag="OO369_004195"
     CDS             1976..2401
                     /locus_tag="OO369_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984226.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
BASE COUNT          651 a          638 c          662 g          676 t
ORIGIN      
        1 tggcttcacc cgtgttaata tcctggataa ctgtgtttga aggatttctg ttaataatct
       61 gctcctgcaa ctcacctgat gagtattagt ggtaatacat aatcttactc gtaaaagagt
      121 ataccttatg tcattaaaca gtcttccggc gtgttgttca atcctgtacc tctgttactt
      181 atctggtacg ttgatatgtg taatgctacg ttaattgcaa tatctggtta tggctttata
      241 ccttcagcat ttaaaatatt ttattaccgg agaaacaggg aagtggaaag actttcggga
      301 taatgtcagg ccattctgac gttaatcaca gtaccaaata acgagagggt aatggaagtt
      361 aaaacctgcg taagttcctc tgttgttcac tggaaaaagt gattgcccac acgcgttata
      421 aaggcctttc cgctgcagcc tggttaacag agcttcaggc gttatatgcg ctgtatggct
      481 gttacgtatg ctgttctttg cctggttctg atacccgtgg aagtcgttac cggaggaaac
      541 acgttcaccc tactacctga gaacatcttc ttcaaaaatt acggcaacgc ttcttcctgt
      601 gttacagaac acgaggaaga aacatgcaaa cttataacac caatcccgct tacgaaatgg
      661 ctccccagct cctggagcac tttaaccagc atctggattc attattcggg gtctattcga
      721 aactgttacc gttcagaatg gattttgctt accgaaaaaa caccctcagc tacagatgtg
      781 cctgcaggta cgccatgtgc gcggagatgc tgcaactgat taacgaggtg ggagagaaac
      841 tggtcgggta tgcctgggtg atggaatata cagaacgtaa agggcttcac attcattttg
      901 tgggctatct gaacggccag agccacagga gttcgtatct ggtatccaga ttgatgggag
      961 atatctggag gcgggttacc gagggtaatg gttactacca ctggtgccgg tttaataaaa
     1021 attatccggt aaacattaac cacgtcattc actattcaga tcacaaagcg gtgaatgatt
     1081 tacgctatgc catcagctac ctggccaaac gggaacagaa agaatgtggc atcatcctga
     1141 aatgcagcgg gcttcctgaa aaaagtaacc ggggtcgacc acgactggac agccccctgc
     1201 cggggatatg tgctctggtg taaacccctg aacgacaagt acgggctggc gctaatcttt
     1261 gctgtcggtt ccggcggcca cttcagcggc actgcgcatt ttttacggcg tggtcattcc
     1321 gtatgaacca tacggagatt taaccatgac acattcttta ccattccttc cggcaaccga
     1381 tacagcacat cccccaatct ccatctctgc ttactgactg ttttttacgt cagtgggcca
     1441 ttgtgtgaca accacagtgc tgtaacagca accagcctgc tttacccaaa ctccagtcca
     1501 gattttaact gaaccgccat tcacaccctg accacgctgc ccgtaccggg acggctgttg
     1561 cctgcgtgtc gtctggcggc tgaggaacat atactatgta cgcaaaatct tttctcgcac
     1621 ttgatggcaa cggacgtctg accggtgccc gtaccgcaca gactgcacct tacgatagct
     1681 acacctgcca cctttgcggc agtgcactca gataccaccc gcaatacgac actgaacggc
     1741 cctggtttga acacgctgac gaagggctga cggagcacgg tcatgagtgc ccttatgtca
     1801 ggccggaacg cagagaaata cggttgatta aacgtctgca gcaattcgtc ccggatacct
     1861 tacccgtagt acgtaaagcc agctggcact gcaggcaatg tcaccacgat tattatgggg
     1921 agcagtactg cacacactgc cagaccgggc gcttcagtga ggagggggga gcagaatgaa
     1981 cggtataaaa acggggctgg ggatcacgcc gggtgagcac attatcagtg ccgactctgc
     2041 gctcagcaga aatatccggc agtgtttctg cctttcctgc catggccggt tgattctgca
     2101 gacagatgct cagggggcgt ggtttgagca tgatttacat gccctgagtg agcaacagaa
     2161 ggcggactgt gtggtgctga atccggagaa gtcccaccct tacattgagg atatggcgat
     2221 gtttttatcg ccattaccgg tcgtgctgga gtggcattgt gtgatgtgtg agcagttttt
     2281 tcatgggaag aagttctgtg aggcctgtgg tacggggatt tattgcagag cggtatgtac
     2341 gaagtctgtt tacagctacc agccagactt gtttgaggac tgcggaggtg cgagtactta
     2401 atccgaaatg cccgacctgc tcagggcgtt cagaggtatt gccaacctgt attttatctg
     2461 gtgtttcgca cccaccccgt tcgtgacaca aaggcgcgac accggatatc cgcagtcctg
     2521 cttacctgaa taaaacccgg ggtattgatt ggtagtgtgg tgcggaatcg taccgtgatc
     2581 tcgcattcgc cgaattcgca ctgtggtgtc aatcaaccat acgcgag
//
LOCUS       DAJTSA010000228         2608 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000228 DAJTSA010000000
VERSION     DAJTSA010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.230"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..357
                     /locus_tag="OO369_004196"
     CDS             <1..357
                     /locus_tag="OO369_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5984227.1"
                     /translation="VFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRF
                     HDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRN
                     GTNTAQEENVVYLNAR"
     gene            619..981
                     /locus_tag="OO369_004197"
     CDS             619..981
                     /locus_tag="OO369_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR5984228.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            978..1907
                     /locus_tag="OO369_004198"
     CDS             978..1907
                     /locus_tag="OO369_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR5984229.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            1904..>2608
                     /gene="gtrII"
                     /locus_tag="OO369_004199"
     CDS             1904..>2608
                     /gene="gtrII"
                     /locus_tag="OO369_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HCR5984230.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNA"
BASE COUNT          750 a          520 c          540 g          798 t
ORIGIN      
        1 gtgtttgtcc acaccaaacc tgcctaccga agcgacggaa caaaaacagc agcggtaagg
       61 aagatgagaa ccgacagcaa caaggcatgg aagggagcgt taaagcgggc aggcattagc
      121 aacttccgct tccatgacct gaggcatacc tgggcaagct ggctggttca gtccggtgtc
      181 tctcttcttg cgcttaaaga gatgggagga tgggaaactc tcgaaatggt tcaaagatac
      241 gcccaccttt cagccgggca tcttaccgag cacgcgagca aaatcgatgc gattataagt
      301 cgcaatggca caaatacggc acaagaggag aacgtagttt acttaaatgc gaggtaactt
      361 attgatttaa atggtgccga taataggagt cgaacctacg accttcgcat tacgaattat
      421 aagaatccgc ttgtaattca aaggattacc ccatcaacac tgcgctcaca cgtcccacca
      481 catcaaaaca tgtaaagcct tgcaagccat tgtgaggcct tatgtgtctc agttttgtct
      541 catcagacat agcaagtatc gatcaattga gacttggatg atagacttca tgcctttcag
      601 agctcattga ttaaataaat gttaaagtta tttgtaaagt acacatctat aggtgtgctg
      661 aacacactta tacactgggt ggtttttggt gtatgtatct atgccgcaca taccagtcag
      721 gctctggcaa acttcacagg tttcgtagtg gctgtgagct ttagcttctt cgcgaatgca
      781 agattcacat tcaaagcatt gactacagcg atgcgctaca tgttatacgt gggatttatg
      841 ggaatattga gtgtgattgt tggttgggca gctgataaat gctcacttcc tccaatagtc
      901 actcttatca ccttctccgc catcagtctg gtgtgcggtt tcgtctattc aaagttcatt
      961 gtctttaggg atgcgaaatg aaaatatctc ttgtcgttcc tgtcttcaat gaagaagaag
     1021 cgatacctgt tttctataaa acggtacgtg aattccaaga gttgaagcca tatgaagtag
     1081 aaattgtatt cataaatgac ggaagtaaag atgccacaga gtcaattatt aacgcgctgg
     1141 ctgtttcaga cccgctagtt gttccgctgt catttacacg caactttggt aaagaaccag
     1201 ccttatttgc agggttagac catgcaagcg gcgatgctgt aattcctatt gatgtcgacc
     1261 tgcaagaccc aattgaggtt atccctcatc ttattgaaaa gtggcaggca ggtgctgaca
     1321 tggtgcttgc taaacgttca gaccgctcaa ctgatggacg actgaaacgt aagacagctg
     1381 agtggttcta taaattacac aacaaaataa gcaccccaaa gatcgaggaa aatgtcggag
     1441 attttcgact catgtctcgt gaggttgtgg agaacattaa actgttgcct gagcgcaatc
     1501 ttttcatgaa aggcatactg agctgggtgg gtggtccgac ggatgtcgtt gaatatgtac
     1561 gcgcagagcg tgttgctggc atctcaaaat ttaatggctg gaaattatgg aatctggcac
     1621 tggaaggtat cacaagtttt tcaacctttc ctcttcgcgt atggacttat ataggcttgt
     1681 ttgttgcaag catttcattt ttatatggtg catggatgat tatagacacc cttgtctttg
     1741 gtaacccagt acgcgggtat ccctccctgc ttgtatcaat acttttcttg ggtggagtgc
     1801 aactgatcgg gattggtgtt ctcggagaat atataggtag aatctatatt gaagtaaaaa
     1861 atagacccaa atacatcata aaaaaatctc atcgaggtaa cccatgatta aaattaattt
     1921 atttaaaaat gcaaatctcc ttgccttcat atcatgtttt gcaatatcaa tttattgtta
     1981 ttgggggtgg ttgtacgatg gaacacttaa tattgatggc gagtttacaa ataattttta
     2041 tcaaacaatc acgcttgggc ggtggtttca cacttttttg cgacattact ttctccctga
     2101 gcctttttca ctttatataa caccattaat agccttatct tttatcatca tttcagcatt
     2161 tataatctgc agatcgctaa agctagaatc ttatgaatta ttgataggta tgttagtatt
     2221 tattaccttc cctcagatct cctatcaatt agagtttctt aaccaagctg atactgtggg
     2281 aattgctttt ctactggcag cgatatcagc aattattttt cactcgcaaa aaaataggat
     2341 tgtgatattt tctggtatag tactgtccat tctttcaatg gcaatctacc aaacattcgt
     2401 aacatatatt attgcattcg tcattgggtt gcagataaat tcgataatac gaaatgagaa
     2461 aaatattcgt gaatcttttt atagttcatg tttatctcta tccctcatag ctttatctac
     2521 cttaatttac ctgctattaa ccaaagctat caagcattat ttttcgcttg aatcgaacga
     2581 gtacatctca aattatatac aaaatgca
//
LOCUS       DAJTSA010000229         2572 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000229 DAJTSA010000000
VERSION     DAJTSA010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.231"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(64..822)
                     /locus_tag="OO369_004200"
     CDS             complement(64..822)
                     /locus_tag="OO369_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR5984231.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="OO369_004201"
     CDS             complement(830..1933)
                     /locus_tag="OO369_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR5984232.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..>2572)
                     /locus_tag="OO369_004202"
     CDS             complement(1943..>2572)
                     /locus_tag="OO369_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5984233.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          711 a          683 c          601 g          577 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat aacggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aa
//
LOCUS       DAJTSA010000230         2550 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000230 DAJTSA010000000
VERSION     DAJTSA010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.232"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..565
                     /locus_tag="OO369_004203"
     CDS             <1..565
                     /locus_tag="OO369_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5984234.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
     gene            523..1200
                     /gene="bglJ"
                     /locus_tag="OO369_004204"
     CDS             523..1200
                     /gene="bglJ"
                     /locus_tag="OO369_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR5984235.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="OO369_004205"
     CDS             complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="OO369_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR5984236.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2167..2403
                     /locus_tag="OO369_004206"
     CDS             2167..2403
                     /locus_tag="OO369_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR5984237.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            complement(2442..2528)
                     /locus_tag="OO369_004207"
     tRNA            complement(2442..2528)
                     /locus_tag="OO369_004207"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2492..2494),aa:Leu,seq:cag)
BASE COUNT          653 a          567 c          653 g          677 t
ORIGIN      
        1 agtcattgct gatttagccg gtgaaagtga agatccacgt tctgtttgtg aacattatta
       61 ttctttaatc tcacaatatc gggaaattca ctgggttttc atggtgtcac gctcctggta
      121 ttcccaggct gtagaactgc tcatgtgccc tacggcgacg ttattgtctg atgttgaacc
      181 cattgagaat ctggtcaaga ccgtacgttc cggcaatacg cacgcagagc gtatcagcgc
      241 catgctgacc tctccggcaa tgactgaaac tcatgatttt agctatcgct ccgtcattct
      301 cactctttca gagcgcaagg tactgcggct attaggtaaa ggatggggca tcaaccagat
      361 agcttcattg cttaagaaaa gtaataaaac tatcagcgcc caaaaaaaca gtgcgatgcg
      421 tcgactggca attcacagca acgctgaaat gtatgcatgg ataaatagcg cgcagggtgc
      481 aagagaactt aacttgcctt ctgtttatgg agatgccgca gaatggaaca cagccgaatt
      541 aagaagagaa atgtcgcact catagaaaaa tgcgtcatga gtagtatcgg tattgagagt
      601 ttattcagaa agtttgcggg caacccttat aagctccata cctataccag tcaggagtca
      661 tttcaggatg ccatgtcgcg gatctcgttt gcggcggtca ttttttcttt ttctgccatg
      721 agaagtgagc gcagagaggg attatcttgc ctgactgagc tggcgattaa gtttccgcgt
      781 acccggcgtt tagttattgt ggatgatgat attgaagctc ggctgattgg ttcattgtcg
      841 ccatcaccgc tggacggtgt attaagtaaa gcgtcaacgc tggagatttt tcatcaggaa
      901 ctctttttgt cattaaatgg tgtacgtcag gcgaccgacc gactgaacaa tcagtggtac
      961 attaaccaaa gccggacgtt aagcccgacg gagagagaaa tattgcgctt tatgtcgcgt
     1021 ggctactcaa tgacacaaat tgccgagcag cttaaacgta atatcaaaac gatccgagcg
     1081 cataaattta atgtgatgtc gaaactgggc gtcagttctg atgcagggtt gttggaggcc
     1141 gaagatattc tgttatgtat gcggcattgc gaagcaagta atgtgttgca tccctattaa
     1201 tccgcatgat gccgggttta cttccccggc agtgctttca tttcagcgta caatagccac
     1261 attgctgcac atccggtaag cgataacgct ggcagcaagt gcggcgcacc agcaggccgt
     1321 cgcgcagtac cacggtacgc cagagtggat tatcttcacc gttcgtgagc gttttctcaa
     1381 aaaagagggc atggcgcagc gattcaacag tagcctcgcc gagcagttgc ttcatctcag
     1441 tgagatacca gttgatcaaa taaccggtat tactccagat aagtttgccg ttgatctctc
     1501 cggtcgcttc tagtgcttgc acaaccggaa ccagcgcctg gctgattaac gtttccattc
     1561 gctgctgcgg cgaatgtggt gttgcgtttt tatcttcaca cacatcgacc cagaaacagg
     1621 cgacgcgtcc ggtttcgtga aactcagcat ggaaatgttc cggcgacaca tctaatgctt
     1681 tttcctgcgt cagtagcgcc agcattaatg gtggcaccat caggccgata taccattgtg
     1741 cccatagtga gatcagcggt ttgttctcgc ggatcatcgt tggttggttg cgatagatat
     1801 gatcggaata gaccgccagt agagaactta gcgcattcgg tgatgaccat tgcgccagcg
     1861 tcatggcgtt aagcggagcg ggttcatcca ggcggataaa ctccagcaaa tgttcacgat
     1921 gtttggcgat catcgcccgt acggcttgtg caagcccagc atcctgcggc tggagatgcg
     1981 ttcgccagat gacatcttca tagagcggtg cggaacgata ggccataatc gtgatagtaa
     2041 tctaaatgat aatgattgct aatcctagcg ataggtttac tcgatagcaa gggatttctc
     2101 tggcttgcaa atgataaaaa ttatcatatg atattggttt tcattatcaa tgaaagagat
     2161 gaaaccatgt tgcaacgtac gctgggcagt ggctggggag tgttgctgcc gggattgctg
     2221 attgcagggc tgatgtatgc ggatttatcg ccggatcagt ggcggattgt cattctgatg
     2281 ggattagtat tgacgccggt gatgctgtat cacaaacagt tgcggcatta cattttgcta
     2341 ccatcgtgcc tggcgcttat tgctggcatc atgctgatga taatgaattt gaatcaggga
     2401 tgacaaaaca gggaagaaac aagaaaggaa gtaaagataa ttggtgcgag gggggggact
     2461 tgaaccccca cgtccgtaag gacactaaca cctgaagcta gcgcgtctac caattccgcc
     2521 accttcgcac agtcatctta ctttttttga
//
LOCUS       DAJTSA010000231         2492 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000231 DAJTSA010000000
VERSION     DAJTSA010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.233"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>2492
                     /locus_tag="OO369_004208"
     CDS             <1..>2492
                     /locus_tag="OO369_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5984238.1"
                     /translation="QEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDV
                     NAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAH
                     QDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIV
                     VGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYY
                     RLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQS
                     LFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERD
                     LRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQM
                     VNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAE
                     NLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGV
                     GELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDN
                     IWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADALAAD
                     RQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTV
                     KEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYSVSSR
                     LSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQQNVQRSL
                     DIQQRSVQQLANTIINSLIQYDDPAAWTEQEQ"
BASE COUNT          730 a          578 c          602 g          582 t
ORIGIN      
        1 caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat gtttaacggc
       61 acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg cctgcgtttt
      121 ggtcgcgatg ttaatgccta taccagctat gacgaaacgg tgtatcaggt gagtttgccg
      181 actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc
      241 gcaacctttg aaaaactcga agtagacgct gaacgtggcg taattactga ggaatggcgt
      301 gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct gctggctaat
      361 acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac ggtcacgccg
      421 gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac ctttatcgtg
      481 gtcggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt aagtaagctt
      541 ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa ccacctgcgc
      601 tttaatatca ttaatgataa agaaaaccgg gtgaacggca tcgcactcta ttatcgcctg
      661 ccaatggtac aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg gagcatgtta
      721 gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt gaagactatt
      781 tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct gtttttccgt
      841 gtaaatgcac gagacgataa tatgcaggat gctgcgaatg cattaatggc agagttggca
      901 accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc tacccgcctc
      961 acctggctga aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat gctgaccagt
     1021 cgcctggcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga gacatatcaa
     1081 ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa atggcagcag
     1141 ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgagct tgccgccaaa
     1201 aaagcattgt ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa caaaaagctg
     1261 gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga cccacaggcg
     1321 gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacact ttccaatggt
     1381 gccaaggtta ttctggcaaa atccgcgggt gaagagcaaa agctacaaat tattgccgta
     1441 tcgaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc gctggcaaat
     1501 aaagcagtta gcggaagcgg cgttggcgaa ctctcctctt ccagcctgaa acgctggagt
     1561 gcggaaaact cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt gctctccgtt
     1621 agcgcgcgga ctaataaccc tgaacctggt ttccagttga ttaaccagcg aatcacccac
     1681 agcacgatta acgacaatat ttgggcatcg ctacaaaatg ctcaaattca ggcgttgaaa
     1741 acgctcgacc agcgtccagc ggagaaattc gcccagctga tgtatgagac gcgctatgct
     1801 gatgaccgca cgaaattact gcaagaaaat cagattgcac agtttactgc cgcagatgcg
     1861 ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt cattgtcggt
     1921 aatgtcgcag aagacaaact cgtggcgtta attacgcgtt acttaggctc aatcaaacac
     1981 tctgattcgc cattagccgc aggtaaacca ttaactcgcg cgacggacaa cgcatcggtt
     2041 actgtaaaag aacaaaatga acctgtggca caggtttcac agtggaagcg ttatgattcc
     2101 cggacacctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt cgcactggca
     2161 aaagatctac gcgttaatat tcgtgaacag gcatctgggg catacagcgt ttcttctcgc
     2221 ctctcggttg atcctcaggc taaagatatc agtcatttgc tggcttttac ttgtcaacca
     2281 gaacgacatg atgaacagtt aacgttagcg aatgaagtga tggttaagcg tttgactaag
     2341 gggatcagtg agcaagaact gaacgaatac cagcaaaacg ttcagcgcag cctcgatatc
     2401 caacagcgta gcgttcaaca attagcgaac actattataa atagtcttat tcaatatgac
     2461 gatcctgcag catggactga gcaggagcaa tt
//
LOCUS       DAJTSA010000232         2410 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000232 DAJTSA010000000
VERSION     DAJTSA010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.234"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..234
                     /locus_tag="OO369_004209"
     CDS             <1..234
                     /locus_tag="OO369_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR5984239.1"
                     /translation="DALRRKLDNGGRVLVKGKCPVPSSAETSSASGMGNDATVELSPV
                     AGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(323..835)
                     /locus_tag="OO369_004210"
     CDS             complement(323..835)
                     /locus_tag="OO369_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR5984240.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1030..1794
                     /locus_tag="OO369_004211"
     CDS             1030..1794
                     /locus_tag="OO369_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984241.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1745..>2410
                     /locus_tag="OO369_004212"
     CDS             1745..>2410
                     /locus_tag="OO369_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCR5984242.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          678 a          539 c          612 g          581 t
ORIGIN      
        1 gatgctcttc ggcgcaagct tgataatggt ggtcgggtgc tcgtcaaagg aaaatgccct
       61 gtgccatcct cagccgaaac ctccagcgcc tccggcatgg gcaatgatgc caccgtcgaa
      121 ctctctccag ttgctggacg aaacgttctc ggtatccggg acggaatcat cagcgaccag
      181 acagcattga gaatgcttca ggagtacatc aggatccaat gccttggggg gtagcggtaa
      241 ttttactcat tatccttcaa atcagattct gttgtcagag gaatggggga agctggattc
      301 gcaagagctt tttatgggca gtttagctaa aaaaaggcaa cacgaagcta aaactaccaa
      361 cataaagtgc tgaccagcta gcagctgaaa tcagactcac tgtatacact ttcactggat
      421 gaagttgcaa cattcctgcc accaaaggag caatgtaacg cagcacggcg ataaagcgcg
      481 aagtgaaaag tattgacaca gagttatttt gcaactgaag gcgaacccgt tctattgtat
      541 tggaacggct cgatattata catgcaagtt gagggatatg cccaattatt ataccaagat
      601 ggtaattaac gatagtaccg caccatgcgc cactcatgac cgtgattcca gcttcccatg
      661 gtgaaagagt cgtcttgctg actgtaatta cagtggccat cataattgat gctggtggca
      721 atatcgccga aatgagtaaa gtggattttg tgaacgctat tataaaaaga agcccccata
      781 ggcgatatgg atgaagtaca aaatagttca tcaaagcatt tatccactcc atcaatgtct
      841 ttccttctgt gaatttttct ttgctattta accgtgatac gacttaattt aggcttaaac
      901 accttacatt tacaaggtac tcctgtgggg ggccctgcca cggagcgtcg caggagtgag
      961 tgatgaggaa gaattaaagc aacctattgt gacaggcagc gataacagta aatcacagga
     1021 agcataatca tggcaaaacc ggactgggag gccatcgaat cggcataccg ggccggagtc
     1081 cttagcctcc gtgatatagg cgagaaatac ggcgttacag aaggggctat caggaagagg
     1141 gccaaaaagc ttgattgggc acgcagtggc ggtacgcagg tttgcaaaaa tggtacgcaa
     1201 aaaaggaaag tgcgtaccag cagaaagcct gccattactg gccttacaca aaaaagtacg
     1261 caactaaaaa cagaatctac accggatacg aaaccgatac gcggaatgcg taccgatccc
     1321 ccgactaacc cattccaacc cggtaaccag caggcattaa aacacggtgg ttatgcccgt
     1381 cgccttctgc tcaaagatga ggtgatagag gacgctaaag cgttgacgct cgaggacgaa
     1441 ttatttcgcc ttcgtgctaa caaccttgtc gctgcagaga atattggtcg gtggttggtg
     1501 tcgctggaag atgctaatgg ggaccaggaa aggaagatgc tgattgaaaa tatcagcgcc
     1561 gccgagaaag caatgatgcg caatacagtt cgtattgagt ccatcgttgg cacgcttgcg
     1621 acggtaggaa aaatatttgc tgatacagcc taccgcaagg ccgccactga taaggtgtct
     1681 ctggaggctg atcgtcttcg ccgtgatgca ggtattgatg atggcaatgg agagcgtgac
     1741 ctcaatgact tctactctga catccaaacc gacgctgaat ccggctttac gtagtttctg
     1801 gactatgcgg gcacgtaaca aagtgcttta tggtggtcgg tcatcgtcaa aatcatggga
     1861 tgccgctggc attgccatat ttctgtcgaa taaatacacc ctgcgttttt gttgtgcccg
     1921 tcagatccag aataaaatcg aagagtcggt gtataccctg ctcaaaattc agatagacag
     1981 gtttggtctg cggcaccgtt tccgtattct gaacaacaaa atcattaacc gggttactgg
     2041 ctcggaattt gttttttatg gattatggcg caacatcgaa gaaattaagt cactggaggg
     2101 gatcgatatg ttgtggctgg aagaagccca cgcactgacg gaataccagt ggaaaattct
     2161 ggagccaacg atccgtaaag agggttcgga atgctggttc atattcaacc ccggacttgt
     2221 tactgatttc gtctggcgca acttcgttgt tgatccgccc gaaggcactc tcatccgcaa
     2281 aattaactat gacgaaaatc cgtttctgtc tgacaccatg cttaaggtta tcgacgcggc
     2341 gcgacgccgt gatccggatg gttttaaaca tgtgtatgag ggcgttccgg agtctgatga
     2401 tgatgcggca
//
LOCUS       DAJTSA010000233         2325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000233 DAJTSA010000000
VERSION     DAJTSA010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.235"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            61..282
                     /gene="kilR"
                     /locus_tag="OO369_004213"
     CDS             61..282
                     /gene="kilR"
                     /locus_tag="OO369_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HCR5984243.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            276..452
                     /locus_tag="OO369_004214"
     CDS             276..452
                     /locus_tag="OO369_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984244.1"
                     /translation="MVMTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYC
                     SEICAEKDQMAHEL"
     gene            527..802
                     /locus_tag="OO369_004215"
     CDS             527..802
                     /locus_tag="OO369_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984245.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            902..>2325
                     /locus_tag="OO369_004216"
     CDS             902..>2325
                     /locus_tag="OO369_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR5984246.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAI
                     VVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEE
                     GKQDDEEKTQPSGAMADEQATAETMEPDATEHHQNTQPLDAQSQVNSVDEKYQELRAE
                     LHEARKNIPPKNPVDADKLLAALR"
BASE COUNT          719 a          531 c          529 g          546 t
ORIGIN      
        1 taagtattaa ataactttcc tgagatttcc tttgtaagac tgtttattaa ggaggcttcc
       61 atgattgctc atcacttcgg aactgatgaa ataccacgtc agtgtgtgac tcctggcgat
      121 tatgttcttc atgaaggtcg gacatatatc gcctcggcaa acaatattaa aaagcgaaaa
      181 ctttatattc gtagcctgac tacaaaaaca tgcatttctg actgcatgat taaagtcttc
      241 ctcggtcgtg atggtttacc tgtaaaggcg gagtcatggt aatgactaag aaaataaaat
      301 gtgcttatca cctttgcaat aaagaaattg aagaaagcaa aatcattaca agaccacttc
      361 atttcatgcg tggagttata ccaacgacgg aaatgaaaaa atattgtagt gaaatctgtg
      421 ccgaaaaaga ccagatggca cacgaacttt aattaactga ctatccgaaa ctgaatttat
      481 gccagcaatg gcagggattc gctcaacctt aattaaggag aaaaacatga ttaccagtta
      541 tgaagccact gttgtaacta ctgatgacat tgttcacgag gttaatctgg aaggaaagcg
      601 tattggctac gtgattaaga cagaaaataa agaaacccca ttcactgtgg ttgatatcga
      661 cggtccatca ggcaacgtta aaacacttaa cgatggtgta aaaaaaatgt gtctggtgca
      721 cataggaaag aatctgcccg cagaaaaaaa agccgaattt ctggcaactc tgattgcaat
      781 gaaattaaaa ggtgaaatct gaaaaaaaga aagcctgcac actgtgcagg cctgaatgaa
      841 gaacctggga catttattca tcactcgcag taattttaat ctgagttgag gttaaaaaac
      901 aatgaacacc gataaacaag tttacccact gtattacgaa gcaaaaaatg acaaagtaag
      961 aaaacgtctc ggtattaaag gcggttttta ctgggctgaa gcgaaaaaat tatccattgc
     1021 catctcccgt ggtgctgttg cgattgacga tgcaggctac gatgaagatg actttaaaaa
     1081 acctgttcgc gtcaatttgc ccgttgttga tgacctcccg ccagaaggcg tatttgatac
     1141 ggaattctgc aaccgttacg aaaaaggcgg ggaagatggc atcacaatgg tatttatcgc
     1201 atcttctccc tctgttcagg acaaaccggc cagcactgac aataccaacg tcaacggcga
     1261 agacatgaca gaaattgagg agaacatgct cctgccagtt tccggtcagg aactgcccat
     1321 tcgctggctt gcgcaacacg gcagcgaaaa accagtaacg cacgtttcac gcgacgaact
     1381 ccaggcatta cacattgctc gggatgaaga actaccagct gttactgccc tggctgtttc
     1441 ccacaaaacc agcctgctcg atccgctgga aattcgcgat ctccacaaac tggtgcgtga
     1501 tactgacaga gttttcccta atccaggcaa ttcaagcctg gggctgatga ctgctttttt
     1561 cgaagcatac atggacgcag actacaccga tcgcggtctg ctgacaaaag agtggatgaa
     1621 aggaaatcgt gtttcacgca tcacgcgcac ggcttccggc gctaatgctg gcggcgggaa
     1681 cctcaccgat cgcggcgaag gtttcgttca cgatctgacg tcactggcgc gcgacgtagc
     1741 cactggcgta ctggctcgtt caatggacgt ggacatttat aaccttcatc cggcacacgc
     1801 taaacgtgtc gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt tccgcgacaa
     1861 attcatcacc atgcctggcg ggatggatta ttcccgcgcc atcgtggttg cgtcagtgaa
     1921 agaagcaccg attggtatcg aggtcatccc cgctcacgtc actgaatatc tgaacaaggt
     1981 actgactgaa accgatcatg ccaaccctga tccggaaatc gtggatattg cctgcggtcg
     2041 ctcctctgcc ccgatgccgc aacgtgtaac agaagaagga aaacaggatg atgaagaaaa
     2101 aacgcagcca tctggcgcaa tggcagatga acaggcaacg gctgaaacaa tggaaccgga
     2161 tgcaactgaa catcatcaga acacgcagcc gctggatgct cagtcacagg taaattctgt
     2221 tgatgagaaa tatcaggaac tacgggcaga actccatgaa gcccggaaaa acattccgcc
     2281 caaaaatcct gtcgatgcag acaaattact ggctgccttg aggta
//
LOCUS       DAJTSA010000234         2263 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000234 DAJTSA010000000
VERSION     DAJTSA010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.236"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            74..774
                     /locus_tag="OO369_004217"
                     /pseudo
     CDS             74..774
                     /locus_tag="OO369_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            776..1381
                     /locus_tag="OO369_004218"
     CDS             776..1381
                     /locus_tag="OO369_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR5984247.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1368..>2263
                     /locus_tag="OO369_004219"
     CDS             1368..>2263
                     /locus_tag="OO369_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5984248.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          549 a          527 c          623 g          564 t
ORIGIN      
        1 ttttctgctc attattagct ccttctgatg ccattctatt tcaggaagga gtgtccgtta
       61 aactcaggct acctcaaacg cccgtgccgg tgtgccgggc agcgatgagg aaacccgtgc
      121 ggactttgaa aaccgccgac gtaattcagt tgcccgtaat gcccggaata ttctggaagc
      181 catccggggt gaaatactct ccacggtaga aaacgtggtg gatgtttacg tcacccataa
      241 tccgaaaaaa acagaacaaa aagccggagt cagtatccat taacacccgg ttcgctttat
      301 gttggcgtgt acggcggcag cccggcagat atcgcggcgg ccatctggcg taaggctccg
      361 ccgggtattg atatgaatgg caacacaacg ttcactgttg cagataagga gtacgatccg
      421 ccgtatcctg aatacgtgat cagctggcag acactcaaac ccgtcagtct gcatgtcagt
      481 gtgacgctga aaaaaagtga ctacctgcct tcagatatta cccgacaggt acagcaatcc
      541 gtgctggacg catttaatgg tacagatggt ggtctgcggg caagagttgc ctctgttgtc
      601 tctgccgggc gctactatgc cggcatttac aaaaccgatc cggaacatat tgatattctg
      661 ggccttactg tgagccgtga tggttcgtca tggacaacgg ctgtcacttt cgggatagat
      721 gagattccgg ttctggatgt gtcggatatc agtgtgaaac tccaggaggc ataacgtgca
      781 gaatgtggct gccactgtgc ttgcgcagta tgctgccagc ccccgactca atgccctcat
      841 taacagcttt aacgcagcgc tttcccccga cagttttatc agtgattttt atggtcttat
      901 ctggaacatc gataccgcag aaaagtatgg tcttgatgtc tggggaaaga ttgtgggtgt
      961 cagtcgctgg ctgacggtaa aggacgattt taattacctg ggcttcagcg agtccaggat
     1021 ggacaccccg gtaatggatg atccctgtcc gtttaatcag gcaccgtttt acaacggaaa
     1081 atcggatacc cggactgttg acctgtctga tgctgtatac cggcggctga tactgatgaa
     1141 agccatgtcg aacattactg actgttccgt tccggatatt aacctgatgc tgagatttat
     1201 gttcggaaaa aaacgccggg cttatgttct gaataatggc gggctgagga tgagttacgt
     1261 ctttgagtcc gcgctctcgt tggcagaact ggcgattatc cagtcgtcgg gtgcactgcc
     1321 atccccgccg ggtgtttatg tttcagtagt tttaaaggag tcccgtaatg aaggccagtg
     1381 ataaaccccg ccagctggcg gtcccctttg cgagtaccgg agataaaaac cgtatcccgg
     1441 acaaggcgac acagcagacc agagagagcg gtaatgctgc gtatgattca ggttttcctc
     1501 cggtgaccat gacagcggtc tcagcgggag gtataccgcc acacggcaag gattttaacg
     1561 gtctgatgta cgatattacc gcagcaatac ggttcgccca ggctggcggt ttgtacacgt
     1621 ataatgccgg ttttgcgggg gccattggtg gatatgcaaa aggagccatt ctcgccggag
     1681 tcgcaacaac agcggtctgg ctgaatacca cggacgataa cctgaccgat cctgaaggct
     1741 ccgacagtgc gggctgggta aatcttcttg aggatccgaa aaggatattc ctgcggcaga
     1801 agaacaatct gtcagacctt cagaataaag ggacggcacg ggataatctt caggtttaca
     1861 gtaaagagca gtcagatcaa cgctatgttc atcgggaagg cgataaaata accggagagc
     1921 tgaaaatccg tggtgttaat gcgctgagga ttttcaacga agcttttggc ctgatttttc
     1981 gtcgttcgga agagtgcctg caccttattc ccaccagtga aggtcagggg gaaaatggcg
     2041 atattggtcc cctgcgcccg ttcaccatta atttgcggac gggtgaaata tccatgtcgc
     2101 ataaagtgtc tgttggcggt ggttcgcagg tcaatggtgc gctgggtatc ggcgttcaga
     2161 acgccctggg ggggaattca attgttcttg gtgataatga caccggattt aaacagaatg
     2221 gagacggtat tctggatgtt tatgctaata gtcagcgtgt att
//
LOCUS       DAJTSA010000235         2226 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000235 DAJTSA010000000
VERSION     DAJTSA010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.237"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            83..706
                     /locus_tag="OO369_004220"
     CDS             83..706
                     /locus_tag="OO369_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5984249.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSARY
                     THTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSAERPYWITGANCEVVDIGCVFTETT
                     SKVTSASALSVTRSGKINANGSIDVATGVSSVNVVSTGVYDVSFSHPLAAVASGYIVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLI"
     gene            complement(767..1939)
                     /locus_tag="OO369_004221"
     CDS             complement(767..1939)
                     /locus_tag="OO369_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5984250.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
BASE COUNT          685 a          396 c          453 g          692 t
ORIGIN      
        1 ttgtgaagga aatggtactc agggggattc cagtacaggt ggtgcgcaat tagctataaa
       61 tggccttaac ggttcgtgcg ctttggtatt aaacaaccca tactttgagg ctaatgccgg
      121 gggggcagac cttgctatag ataatactgg gacgcgccct gtaactgtgg ttataaacgg
      181 tggaaatttt catcgcgtga gcagtgcaag atacacgcac acgaatattc aagtaacaag
      241 ctcaggtggg ggaaaagtta cagtcatact caacggcaca acgttccaga gcgcagggga
      301 ttatcagcca agtgcagaac gaccatactg gataacagga gctaattgcg aggtagttga
      361 cattgggtgt gtttttacag aaacaaccag taaggtaaca tcggcttctg cattgtcagt
      421 cacacgctcg ggaaaaatta acgccaatgg tagtatcgac gttgctactg gtgtttcatc
      481 ggttaacgtg gtttctacag gtgtgtatga tgttagcttt tctcatccgc tcgcagcagt
      541 tgctagcggt tatattgtgc agataacacc tatatctgcg cctgattcag ttagttgtga
      601 tgttacttat attggcgtag atacatttag ggtgaccctg cgcaatacgc ttagcggggc
      661 aggaatctcc agttcgtttg catttagcat taccagacta atataaatac attcgccgcc
      721 ccatttggac atatatgtag ggcggcttat gtttattgtt tattgtttat tgattgttgt
      781 ttgttaatgt gggtattaat tcttttacta aagttgtggt ttgttttgtt aatttgatga
      841 atggcgtctc tattaattta aaagttaggc atgatgtgaa cgtaataaga aatgcagttg
      901 tgcttaccag tatgataaat gtattttcag tgtaattatt aggaataatc cacgtcatta
      961 ggcaataaag gaatatccca tgcagaagat aaacgctgta ggtagtttct cctagctttc
     1021 tggttatatt taatcttaat attccataca aatcacaacc tgatgcgata atgataaatg
     1081 ttattccaca aagaatcagt ggtagcggtg cgtaagttgt tttgaaatac gtcatttcaa
     1141 aaaccattat cgcagttatt ataattggtg ttacttttgc tttggcaatt ccattgacta
     1201 tctttgattt atttagcaaa aatgccagga gcccaaaaag aaatgatgct atgtgtattt
     1261 tactgaaaaa taaaatgaaa acaaatagcg atattgcgct aatcataacc atgtaaatac
     1321 tgacttttct ttttatgagc gcggaaatta ccggaagaga aaaataaaag aaccattcat
     1381 atacaagtgt ccatgttaca gcggcattga tagtaaatga atcttttacg tcattaatgt
     1441 tcggcatacc tagcgcagtg aatggcaacc acttcattat tgacacaaaa agctcttctg
     1501 tggatacctg cattctccat ccagacttaa aacctacaat gataaaaatg agacataggc
     1561 taactatgaa cattggggtt aatcgtagta atcttgatac ataaagcctt gtccagtcct
     1621 gatctccaga gataatcttt gaaaagaaca gataaccagt aatcataaaa aagaatgaca
     1681 caccaacttg gcctaaattt gctaacagat ttgatgaagg tgcttcccat actccagatg
     1741 acaagtatcc attccaaatt gctgcgtgat gaataagcac gaaaattgct aagaaccccc
     1801 gcattccatc tatttgatta ttcctgccgt gctcaacaag atttattggg gaaattttat
     1861 caaataaccc cacagctaat aacgacatca atatcacgga tgttattaat aataaactgt
     1921 taatttcaat catatgcatg ctctattacg caattaaaat ttctccaagt atactgactt
     1981 ttagttaatg atcatatagt tatgtgtaac tgatgaaaca aaacggagac acaccaagct
     2041 ttgcactgga ttgcaaggct ttgtgctctt ctgtgaatgt gtggctatat gtttgaagat
     2101 cgttgtgccg tatttgtgac atatatatgg caacatcatg catcaacttt ctgtttgtgt
     2161 catcaactat agcttagtga atgcggttaa tgcttgctaa aacagatagt tatgattggt
     2221 gctaca
//
LOCUS       DAJTSA010000236         2218 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000236 DAJTSA010000000
VERSION     DAJTSA010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.238"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="OO369_004222"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="OO369_004222"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR5984251.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="OO369_004223"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="OO369_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR5984252.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..>2218)
                     /gene="arsR"
                     /locus_tag="OO369_004224"
     CDS             complement(1881..>2218)
                     /gene="arsR"
                     /locus_tag="OO369_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR5984253.1"
                     /translation="IQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISRH
                     LALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQNC
                     SGDSKNICS"
BASE COUNT          589 a          611 c          555 g          463 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaggac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgg
//
LOCUS       DAJTSA010000237         2176 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000237 DAJTSA010000000
VERSION     DAJTSA010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.239"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(209..373)
                     /locus_tag="OO369_004225"
     CDS             complement(209..373)
                     /locus_tag="OO369_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984254.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1275..1559
                     /locus_tag="OO369_004226"
     CDS             1275..1559
                     /locus_tag="OO369_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5984255.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1547..2032
                     /locus_tag="OO369_004227"
     CDS             1547..2032
                     /locus_tag="OO369_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5984256.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          560 a          461 c          618 g          537 t
ORIGIN      
        1 gacattattt ttgtggaacc ggaggtaaca gaccagacgg tttaaatgag ccggttactt
       61 taaacgggtg atggacagca cctcaaaagt tcagatggat tatatttgtc tgcaataccc
      121 cgggtatttt cgatttgcga aatggatgga acgacttgct caggggatag ctgatggagt
      181 tactgaagta ccgaaggacc actaaaaatc acaggccggg aatttcggcg tttccggcgt
      241 aggtgtgcca cgaatgcatc cagtggcgtg atgttggatg caatgctgta tgagagatga
      301 ggggcggcgt ccagacgcag ccttcaaacc gccttgagct gctgccgtta agagcggtgg
      361 tggtgagcgt caaggttaaa aggcgcagaa catgtgtcag gtaggtgttg cggagatgaa
      421 cgaaagtgaa ctgtcgatga agtgtcgaaa ggaacctcta ggcgatgtcg aaaatgtgct
      481 atctacgcag cgcatgacga gtccagcaga tatctggtac tggctgggcg gcatccggca
      541 tataggcagc atgaacgcaa cacaggctct cgtacggaac gtgggaacct gtcgtctcga
      601 tgctaaggga gcggcgcgga gcagaggcac tgcaagcgcc tgagtaccga tgcgagacac
      661 aggggcagat ggcttcgtag tagtgaggaa gtgcctgtaa tgggcatgga gcgaaggggg
      721 tcagtcgttg gggctggttt atcggtcaac cggatagccg ggaggagccc atgagtcagc
      781 caaagccgtt cgaggtctct aagtatgcgg tttggaaagc ataccagcgg gtcaaagcta
      841 atcgaggtgc ggcaggtgtc gatgggcagt cagtcgaggc gtttgaggtt accaatcgaa
      901 gctatacccg gtaatggcgc aggttgacct gcacttggcc aagtggattg cccgcaaaca
      961 caagcgagcg cgaggtagtc tggtgcgtgc attcgagtgg ttactgcgaa ttcgtcacga
     1021 ctgcccgacg ctgtttgcac actggtgctt agcttacgat acttgaatgg ttcaacgaca
     1081 agagccgtat gacgcgagag tgtcacgtac ggctccgtgg gagcgtaggg gggcggtgcc
     1141 cctgcgccac ccgatatccc ctttgagtgc ggttacgtcc acagaggtaa atgccgctga
     1201 cgcggcatct gtaatctgtt actttgtgta tatcatttgc tatatattgt caatatgcta
     1261 ttgttgtaaa gcaaatgata tacggaggct ttatgaaatc aggtgttcag ctcaatctca
     1321 gagcaagaga atcacagcgg atactcattg atgctgctgc agaaattctt cataagtcgc
     1381 gtacagattt cattctggaa atggcctgta aagctgctga agatgttatt ctcgaccggc
     1441 gggtgtttaa ttttaatgac aggcaatatg aagagttcat tgaaatgctt gatgctccgg
     1501 ttgcagatga tcctgccatc gaaaaattgc tggcaaggaa acctcaatgg gatgtgtaac
     1561 tgcaccagaa cctttatcct catttcatca ggtcgctgaa tttgtcagca gtgaagctgt
     1621 acttgatgac tggttaaagc agaaggagct aaaaaatcag gcaatcggag ccacccggac
     1681 ttttgttgta tgcagaaaag gtacgcaaca aatagtcggt ttttactcac tggccactgg
     1741 tagtgtcaat catacagaag ctacaggtaa ccttcggcgc aatatgccag accccattcc
     1801 tgtaattata cttgcacgcc tggctgtaga tgtgtcattt cgaggaaaag ggcttggcgc
     1861 ggatttactt catgatgcag ttcgtcgttg ttacagggtt gccgaaaata tcggtgtgcg
     1921 tgcaattatg gttcatgcgc ttacggaaaa cgcaaaacag ttttatattc atcatggttt
     1981 taagccatca aaaactcagg tgcaaacatt gtttcttaaa ctccctcaat agggagtaat
     2041 gccgatcagt taaggatcag ttgaccgatc cagtggctgt gtaagaatcc ggaaacgctc
     2101 actcgtttcc ggatttttct gtgcacagta aaagaccacc tcctgaggag gtggcttttc
     2161 agaatgcccc ctataa
//
LOCUS       DAJTSA010000238         2162 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000238 DAJTSA010000000
VERSION     DAJTSA010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.240"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            80..259
                     /locus_tag="OO369_004228"
     CDS             80..259
                     /locus_tag="OO369_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984257.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            311..505
                     /locus_tag="OO369_004229"
     CDS             311..505
                     /locus_tag="OO369_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984258.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1304..1640)
                     /locus_tag="OO369_004230"
                     /pseudo
     CDS             complement(1304..1640)
                     /locus_tag="OO369_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          688 a          444 c          450 g          580 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctaggtag cgtaaggcac tacagcgcat
       61 tgctttacga gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact
      121 gcaacaccgt atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga
      181 cccattgcga tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa
      241 aacacgtcca ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga
      301 ggaaaaccgg atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca
      361 acgggagttt gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt
      421 tccacttgaa gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt
      481 cttagaatgc aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg
      541 gcatcgttat cagcgcactt gattttcttt atattcactg acgagcttgc cggggcacct
      601 ttgtttgccg gtcattctgg tatcatgcag atatttacgg cgtacaagcc cactagcatg
      661 acattgtaat aaggcctgag cctaacgctt taatgtgtgt catacactca gtggcgtaat
      721 cgttgacaat atggtgcatg taatcgtaag cgtccagcga aatccatctg gtcataatct
      781 gaagcatccg acaaagtggt gtccaccaaa taagtgatct gcgcccaata tcctgagccg
      841 gaactttgac aaaggccaac gaaaaatggg agcggatgtt acagagttct cggtacaggg
      901 gaaaagtggt acctgtcgcc agttctccga tctgtttaac cgggaaatta tctcctacag
      961 cttgtcagaa aggtcggtga tggagcatcg ttaatacgat gctatacgat gcattcacag
     1021 tgcccggcca gaggatgccc cgtcgctgca tatggatcag ggttggcaat atcgaatcgc
     1081 aggctatagg caaagttaaa gccccatgga gtagcacaaa atatgccgca caaaggaaac
     1141 ggtctgaata acgcagtgat gaagaacttc ttcagcacac tgctaaaacg catagtgatc
     1201 gagcgctgat tctggcgaac aactgaacta atacatcaga atctgcatta tgttaaataa
     1261 atatgaaaag atggtttaaa taccccgtta cttttgactt acactatacg gtatcgcatc
     1321 gtttaatatt cgcaccggcc agatttttat ttctattagt tgtcacaata ctgaatgcgt
     1381 acgaccacag tattctggct cctgtgtggt tatgctttaa ttctgcgttc cgggcagata
     1441 agcagttgct tgcaggaatc cttcttgtgt taatgtcagt tccccttttt accagtgctg
     1501 atttccacat tccgtccaac agagcttata gcctttccct ggattatagc attgtccggc
     1561 tgaagttctt tttgaataat aatagaagca ctgctggcag atccagtccg tttttcataa
     1621 cccactgtac tgataaccat aatctaatca gtagaaattg agtcgaaaat aagcactact
     1681 ctatacagga taattagagg tcagtttgat tattcacaat tcatcatcag cattttctat
     1741 ttctgacgaa atcaacatga aaataaccat atatgataat tattataata acggctttaa
     1801 ttggaataca tatattacaa cgtattatat ataattggta ttctgggaac tatattctca
     1861 aaatacagta gaaacgaggt atgtttctgg tggaaaggac agtgggatta aaaagtaaga
     1921 attgataaaa aaaacgccag caacacatag tgccggcgga gggaataccc catggagaaa
     1981 atgtgatgcc tagagcatca tgaatatata attaaaaata gttagcgttg tcaatacttt
     2041 cacaaaaata attttcgtag tatagaaaga tatttttatg catgacctac ctgaatttgc
     2101 tccgggtaga ggttataaat aaaaattgaa tcacgacaaa cacaatattc atagtatggc
     2161 ga
//
LOCUS       DAJTSA010000239         2155 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000239 DAJTSA010000000
VERSION     DAJTSA010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.241"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            146..331
                     /locus_tag="OO369_004231"
                     /pseudo
     CDS             146..331
                     /locus_tag="OO369_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(431..640)
                     /locus_tag="OO369_004232"
                     /pseudo
     CDS             complement(431..640)
                     /locus_tag="OO369_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(690..869)
                     /locus_tag="OO369_004233"
                     /pseudo
     CDS             complement(690..869)
                     /locus_tag="OO369_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1393..1677
                     /locus_tag="OO369_004234"
     CDS             1393..1677
                     /locus_tag="OO369_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCR5984259.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            1677..1952
                     /locus_tag="OO369_004235"
     CDS             1677..1952
                     /locus_tag="OO369_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR5984260.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          599 a          536 c          517 g          503 t
ORIGIN      
        1 cggtttacgc ttaccgcagg acaggtggta actgaaaaat agtttgctga ggcgactgga
       61 aacagaaggg caagatcgat tccgacagca gtgatggtca gctacctcat cgataacttt
      121 gcaacagtgc ccttcttgaa gcatcatgct tcatgaagaa aaactggcac gtcatcaacg
      181 taaacaggcg atgtataccc gaatggcagc cttcccggcg gtgaaaacgt tcgaagagta
      241 tgacttcacg ttcgccaccg gagcaccgca gaagcagctc cagtcgttac gctcactcag
      301 cttcatagag cgcaatgaaa acatagtttt acaggggaca tctgacatca caaatccccg
      361 ggtggggatt tgtgtataag agacagccca tggaggaagg ggatatatcg cagctctgac
      421 gactcactcc ttatttaccc gggaaatact tctccagcaa tgcatacagg atggcagcgg
      481 tttcagcatc cattatgccg tcataatttt tctgccggaa atgaagctga aaagcgcgga
      541 caagcgcctt atatccgtca tccgtctcca caccagaaat actgtatcca taggtcttca
      601 gccgtgacac gatatcaacc ttatccggaa tgccctgcga ttcaaaaaat aaatatttag
      661 tcagtaattt tatgtttttc ctgaaaaaat tactcttttt cgaactccag tgagcgttca
      721 cgactcatca gtgtgaaaat gatggcaaaa acagcagaca gtatcagtac gataaatgtg
      781 gccccccatc ccagataatc gacaacaagt cccattccca gtgtcgcaac aattgagccg
      841 aagatatagg aaaatgtgcc tgacagccct aacagaataa gtttacgcgt aaatcgccct
      901 gaatccagaa gagcattaag tatcacataa acaggttcca taccagtcct gaccgcatca
      961 ggacgccagt aaataaaatc tcgtagtcca ccggcatcag agacaagagt aatcgtcgtt
     1021 accggtattt cgactggttt gtactggtgt gagcggacaa aactactgta cgtctctcat
     1081 acacgctcgt aagcggccct cacggcccgc taacgcggag atacgccccg actgcgggta
     1141 aacccttgtc gggaccactc cgaccgcgca cagaggcatt ttcatcgctg acaggccgca
     1201 tggcctcgca gagccggagg gggactggtg atattcggac gaccgtaccg gcttgcgccg
     1261 ggcttcggcg gttttacacc tgtgctacat gtaaccaccg tatgccgcct tatatgcgct
     1321 gactcgcatt atttgccttg ttccgtctga aagtaatcac tatgattaaa tatgattaac
     1381 aactaatcgg gtatgcaaat gaaaaacaat accgcacaag caacaaaagt aattaccgcg
     1441 catgtgccat tacctatggc tgataaagtc gaccagatgg ccgccagact ggaacgctcc
     1501 cggggctggg ttatcaaaca ggcgctttct gcatggcttg cccaagagga ggagcgtaat
     1561 cgcctgacgc tggaagccct ggacgatgtg acatccggac aggttatcga ccatcaggct
     1621 gtacagtcct gggcggacag cctcagtact gaccatctgt taccggtgcc acgctgatgg
     1681 aactgaagtg gaccagtaag gcactttctg atttggcgcg gttatatgat tttctggtgc
     1741 tggccagtaa acctgctgcc gccagaacgg tgcagtccct gacacaggcg ccggtcattc
     1801 tgttaactca tccacgtatg ggagaacagt tgtttcagtt tgaacccagg gaggtcagac
     1861 ggatttttac tggcgagtac gaaatccgtt acgaacttac tggccagact atttatgtat
     1921 tgcgcctgtg gcacacacga gagaacaggt agattgggtg tggaggcaac tttaacatag
     1981 cctgcaggta ttaattctgg agcattgtta tcaggccaga tattttgcat tccattaaat
     2041 aatcttgtag gagataaaat aaattacgat gaaataacga aaattaccgc tcgtgactgg
     2101 cggcaatatc gcgctccggg ctggcaaata acccatcaga aacgttattg ccaga
//
LOCUS       DAJTSA010000240         2092 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000240 DAJTSA010000000
VERSION     DAJTSA010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.242"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="OO369_004236"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="OO369_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR5984261.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..2016
                     /gene="mcbR"
                     /locus_tag="OO369_004237"
     CDS             1351..2016
                     /gene="mcbR"
                     /locus_tag="OO369_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR5984262.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          577 a          444 c          559 g          512 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca
      721 ccacgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg tgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgcccatc
     1801 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat
     1861 gaagcgatta acccagcgga attacgggag cgcatagaaa actatcatct attactcgct
     1921 gcgttaaaag caaaagacaa agagggatgc aaacattgtc ttgctgaaat tatgcaacaa
     1981 aatattgcta ttttatacca gcaatacaat cgttaaatta aatgcccggc aaataccggg
     2041 catcaagtaa ttactcaaat cgccacgcaa tattcatgcc gacaccataa tt
//
LOCUS       DAJTSA010000241         2084 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000241 DAJTSA010000000
VERSION     DAJTSA010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.243"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..796
                     /locus_tag="OO369_004238"
                     /pseudo
     CDS             1..796
                     /locus_tag="OO369_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            911..1252
                     /locus_tag="OO369_004239"
                     /pseudo
     CDS             911..1252
                     /locus_tag="OO369_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1235..2072
                     /locus_tag="OO369_004240"
                     /pseudo
     CDS             1235..2072
                     /locus_tag="OO369_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          515 a          504 c          631 g          434 t
ORIGIN      
        1 gggccttgcc cgtagcagtt atttttatca ccgggctgca ctgaaagccg gtgataaata
       61 cgccacgata cgtaagatgc tgacagatat ctttaacagt aattaccagt gttatggcta
      121 tcgtcgcctg catgcgatgc tcaggcatga gggggggcgg ctatcagaaa aggttgtacg
      181 cagacttatg gtggaggaac agcttgtcgt cagtcgtaac cgtcgtcgtc gctacagctc
      241 atattgcgga gaaatcggac cggctccgga taaccttatc gccagagatt ttaaggcgga
      301 gcaacctaat cagaaatagc tgacagatat cacggagttc cagctccctg caggtaaagt
      361 ctggctgtca ccagtggtgg actgcttcga tggaaaagtt gtgagctggt ctctcagtac
      421 acgccctgat gctgaactgg tcaacactat gctggatagc gctgtcgaaa cgttaaatgc
      481 tggcgaacga ccggtgatac acagtgacag aggtgggcat tatcgctggc caggctggct
      541 ggaaagagtg aatgcagcag gtcttattcg ctcaatgtcc cgtaaaggat gttcacctga
      601 taatgctgca tgcgaaggct ttttcggcag actgaaaacg gaaatgtatt atgggcgtaa
      661 atggtcgggc atcacgccag aaaagttcat gcagcaagtg gatgcttaca tcagatggta
      721 taacgagcgg cgtataaaat tatcgctggg tgccgtcagc cccaaaatgt accgccaaca
      781 atgcgggctg gaatgataaa gcagtccagg aaatcgtccg catcccccct tcaccatttg
      841 gattgtccgc tccacccaac atgttgtttc cttaaggttc ctacaccaca aaggaaactc
      901 tcatgctgcg cagtggatgg ctgacgttgc cgacaggcgg gaacttcgcc agttcaggca
      961 gacaccggaa cagcgcttca cgcaggaaca ggagcatctg cagccgctgc tgggtacgga
     1021 cttcgatatc cgtcatgtct cctgggatgg ctatatcgag gttggcggga accgttacag
     1081 tgtgccggaa agcctgtgtg gtcagctggt atcaatacgg atctcgctgg atgatgaact
     1141 gcggatctac agtaatgagc aacaggtagc atcgcaccgt ctctgttcgg ctgcatacgg
     1201 ctggcagacc gtgccggagc atcacgctcc actctggcag caggcttcgg agcgcatggc
     1261 cgaatggctg ggggagatac agaaacgggt aatcactgtc tgtgaccggg aagcggatat
     1321 ctggcattac ctgtattata aagtcagcca cggacagcgt ggtgcgtgct gcacagaatc
     1381 gccggctgga agaggcaccc ggcaagctct tcgaactgcc ggaagtcctg gcaaccgcag
     1441 gaagccacac gctgaatgtg atgcaaaaag gcgggcgggc agcccgtcag gcccggatgt
     1501 tcatcagtta cagcgaagtc agcataaaaa atcccgacaa cagcggccag gcgctcccgc
     1561 tcacgtatgt ctgttgccgg gagcaggcag aggacggtgc ctgctggcat ctgctgacgt
     1621 caggaaaagc ggcgagtgcc gccgatgcac gacgtattgt cagccattac gagcgacgct
     1681 ggctgacaga ggaataccac aaggcgtgga aaagtggtgg tacatggaat cgctgcgaat
     1741 gcagacccgg gataaccttg agcgcatggt ggttatcctg gcgtttatag cggtacgggt
     1801 gctgggcctg cggcagggag gtgtcagtga agaaacacaa aatgacagct gtgagaaaat
     1861 actgacgccg acagagtgga aactactgtg ggttaagctg gaaggaaagc cgcttccggt
     1921 gcaggcaccg accctgaaat gggcctgggg cgatggcatg acagcaaacg cacaggtcgt
     1981 cccggttggg gcgtcatgtg ggatggctgg tcagacttca ggatatggtt gagggttacc
     2041 tggtgatgaa gtctcttgat caggagctct gatcaagaga cagc
//
LOCUS       DAJTSA010000242         2076 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000242 DAJTSA010000000
VERSION     DAJTSA010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.244"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            44..2076
                     /gene="sat"
                     /locus_tag="OO369_004241"
                     /pseudo
     CDS             44..2076
                     /gene="sat"
                     /locus_tag="OO369_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
BASE COUNT          755 a          363 c          413 g          545 t
ORIGIN      
        1 ttaagtgata ctggttgtct ggagattcag ggggccagtc tactcatacg gcatgatgat
       61 ttataaaaat gataaaacat ttcgtaatct tgaaatattc ggagacagcg gctctggagc
      121 atacttatat gataacaaac tagaaaaatg ggtattagtc ggaacaaccc atggtattgc
      181 cagcgttaat ggtgaccaac tgacatggat aacaaaatac aatgataaac tggttagtga
      241 gttaaaagat acctatagtc ataaaataaa tctgaatggc aataatgtaa ccattaaaaa
      301 cacagatata acattacacc aaaacaatgc agataccact ggtactcaag aaaaaataac
      361 taaagacaaa gatattgtgt tcacaaatgg gggaaatgtt ctgtttaagg ataatttgga
      421 ttttggttgc ggtggtatta tctttgacga aggccatgaa tataacataa acggtcagag
      481 atttacattt aaaggagcag gaattgatat cggaaaagaa agcattgtaa actggaatgc
      541 attgtattcc agtgatgatg ttttacacaa aataggcccc ggtactctga atgttcaaaa
      601 aaaacagggg gcaaatataa agataggtga aggaaatgtt attcttaatg aagaaggaac
      661 atttaacaat atataccttg caagcggaaa tggtaaggta atactaaata aagataattc
      721 ccttggcaat gatcaatatg cggggatatt ttttactaaa cgtggtggca cgctagattt
      781 aaatggacac aatcagactt ttactagaat tgccgccact gacgatggaa caacaataac
      841 taactcagat acaaagaaag aagctgttct ggcaatcaat aacgaagact cctacatata
      901 tcatgggaac ataaatggca atataaaact aacgcacaat attaattctc aggataagaa
      961 aactaatgca aaattaattc tggatggtag tgtcaacaca aaaaatgatg ttgaagtcag
     1021 taatgccagt cttaccatgt aaggccatgc aacagagcat gcaatattca gaagcacagc
     1081 gagtcattgc tccctggtat ttctttgtgg aacggactgg gtcaccgttt tgaaagaaac
     1141 agagagttca tataataaaa agttcaattc tgattacaaa agtaataatc agcagacctc
     1201 atttgatcag cctgactgga aaaccggggt gtttaaattt gatacattac acctgaacaa
     1261 tgctgacttt tcaatatcac gcaatgccaa tgttgaagga aatatatcag caaataaatc
     1321 agctatcaca atcggcgata aaaatgctta cattgataat cttgcaggga aaaatattac
     1381 taataatggt tttgacttca aacaaactat cagtactaat ctatccatag gagaaactaa
     1441 atttacaggt ggcatcactg cacataacag ccaaatagcc ataggtgatc aagctgtagt
     1501 tacacttaat ggtgcaacct ttctgaataa tactcctata agtatagata aaggagcaaa
     1561 agttatagca caaaattcca tgttcacaac aaaaggtatt gatatctccg gtgaactgac
     1621 tatgatggga atccctgaac agaatagtaa aactgtaacg ccgggtctcc actacgctgc
     1681 tgatggattc aggctgagtg gtggaaatgc aaatttcatt gccagaaata tggcatctgt
     1741 caccggaaat atttatgctg atgatgcagc aaccattact ctgggacagc ctgaaactga
     1801 aacaccgact atatcgtctg cttatcaggc atgggcagag actcttttgt atggctttga
     1861 taccgcctat cgaggcgcaa taacagcccc caaagctaca gttagcatga ataatgcgat
     1921 ctggcatcta aatagccagt catcaattaa tcgtctagaa acaaaagaca gtatggtgcg
     1981 ttttactggt gacaatggga agtttacaac ccttacagtg gacaacctta ctatagatga
     2041 cagtgcattt gtgctgcgtg caaatctggc ccaagc
//
LOCUS       DAJTSA010000243         2050 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000243 DAJTSA010000000
VERSION     DAJTSA010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.245"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            42..1238
                     /gene="hemY"
                     /locus_tag="OO369_004242"
     CDS             42..1238
                     /gene="hemY"
                     /locus_tag="OO369_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR5984263.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWRTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
BASE COUNT          557 a          497 c          509 g          487 t
ORIGIN      
        1 caaggtgata ctccggcagc cgcgccgcaa ggagaataac catgctaaaa gtgttattac
       61 tctttgtttt gctgattgcg gggatagtgg ttggcccgat gattgccggt catcagggtt
      121 atgtgctgat ccagaccgac aactacaata tcgaaaccag cgtcacgggc ctggcgatca
      181 tattgattct ggcgatggta gtgctgtttg ccattgagtg gctactgcgg cggatcttcc
      241 gcactggcgc gcacacccgt ggatggtttg tcggacgtaa gcgtcgccgt gcgcgtaagc
      301 agaccgaaca ggcgctgctg aaactggcgg aaggtgatta tcagcaagtt gaaaagctga
      361 tggcgaaaaa tgccgatcac gctgaacaac cggtggtgaa ctatctactg gctgccgaag
      421 ccgcgcaaca acgtggtgat gaagcacgcg ccaaccaaca tctggaacgc gcagcggagc
      481 tggccggcaa tgacaccatt ccggtagaaa tcacccgtgt acgtctgcaa ctggcccgta
      541 atgaaaacca tgctgcacgc cacggcgtgg ataagctgct ggaagttacg ccacgccatc
      601 ctgaagtatt acgtctggcg gaacaggcgt atatccgcac aggggcatgg agttctctgc
      661 tggatatcat cccatcaatg gcgaaagccc atgttggtga tgaagaacat cgtgcaatgc
      721 tggaacaaca ggcatggatt ggcctgatgg atcaggcgcg tgccgataac ggtagtgaag
      781 gtttgcgtaa ctggtggaaa aaccaaagcc ggaaaacgcg tcatcaggta gcgttgcagg
      841 tggcaatggc ggaacatctt attgaatgtg acgatcatga tactgcccag caaattatca
      901 tcgacggcct gaaacgccag tatgacgatc gcctactgct gccgatcccg cgtctgaaaa
      961 ccaataaccc ggaacagctg gaaaaagtgc tacgccagca aatcaaaaac gtcggtgatc
     1021 gcccgctgtt gtggagaaca ttgggtcagt cactgatgaa gcacggtgaa tggcaggaag
     1081 catcgctcgc cttccgcgcg gcgctgaaac aacgtccgga cgcctacgat tacgcatggc
     1141 ttgccgacgc gctggacaga ctgcacaagc cggaagaagc tgcagcgatg cgtcgcgacg
     1201 gtttgatgtt aacgttgcag aataacccgc cacagtagtt ccttctcacc cggaggaaag
     1261 cacctccggg acctttctga tgcataaaaa aacgcctgct cttattacgg agcaggcgtt
     1321 aaaacaggtc tgtatgacaa caagtgggtg cttcactcaa cgttgtgtcc atggtgtctg
     1381 atgaggcata agcgacatct gtcagtggac gataagcacc gtaaacggct ctgcgtcatt
     1441 ccggagttta tgaggcacta aggcgaacat aagagatgga atgagcatct actcgtttat
     1501 tatgccacag agaatcggga aataacatcc cttaacactt gttatgagat aattctgtaa
     1561 tcctctttgc ttcctgagta ataacttcct gagtgaatat ttaacctgag cttgatccta
     1621 cacatactta ttatgaatga taaaattcat tcaattaata acacatatat taattgccgt
     1681 taaaactaaa aacagcatca ataatcaacg cgatataata aacctgcctt acatatcaac
     1741 tgcgccagag gtaggattga aaacgctctc ctgattttcc aattcatttt ctggataaat
     1801 aaataattta tttttgtcac tattatttat gtaatcatcc tgtcagggag agggatctca
     1861 attatcaatg cttaattacg tcatcatttt gatgacatga aaccggagca ttttgcatgg
     1921 aattttcgtt ttcacccaaa cgtcttgttg ttgctgtcgc cgccgctctt cctctcatgg
     1981 ccagcgcagc agataccccg tcaactgcta gactggcccc ctgaatctcc agacaaccag
     2041 tatcacttaa
//
LOCUS       DAJTSA010000244         2050 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000244 DAJTSA010000000
VERSION     DAJTSA010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.246"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..168)
                     /locus_tag="OO369_004243"
     CDS             complement(<1..168)
                     /locus_tag="OO369_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984264.1"
                     /translation="MRKLKMMLFGASLIMVVGCSSKENALCHPQPKPPAPPAWAMMPP
                     SNSLQLLDETFS"
     gene            complement(385..918)
                     /locus_tag="OO369_004244"
     CDS             complement(385..918)
                     /locus_tag="OO369_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5984265.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(984..1613)
                     /locus_tag="OO369_004245"
     CDS             complement(984..1613)
                     /locus_tag="OO369_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984266.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1617..1832)
                     /locus_tag="OO369_004246"
     CDS             complement(1617..1832)
                     /locus_tag="OO369_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5984267.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          523 a          543 c          454 g          530 t
ORIGIN      
        1 cgagaacgtt tcgtccagca actggagaga gttcgacggt ggcatcattg cccatgccgg
       61 aggcgctgga ggtttcggct gaggatggca cagggcattt tcctttgacg agcacccgac
      121 caccattatc aagcttgcgc cgaagagcat cattttcagc tttcgcatca gctaactcct
      181 tcgtgtattt agcatcgagt gcatcagcat cacgctggcg ctgctgcatg tcagtaatgg
      241 ttgcgttcgc cagcttcagt tctccggcgt ttttgtcgcg ctgctctttg taggtaatgg
      301 cgttatcacg gtaatgatta acagcccatg acaggcagac gatgatgcag ataaccagag
      361 cggagataat cgcggtgact ctgctcattg atctatcccc caacaggcta atgcgctttc
      421 ctggtcacga cgaataacct gtccatagca gttatttgaa cgtatgcggc aatcgcgccc
      481 aacatctttt atccaccagc gaatcgcctc gcatgcgccc ttacgatcac cggcattcag
      541 ccgcttataa aacgtcgacg ggaaacactt accggggcca atgttatagg ggcaaaatga
      601 cgctataccc gctttctggg gttcggtcag aggtacttta atattgcgct ccacccatgc
      661 cagcgcctta tcacgttcaa tagcgttaac ctggtcacat ttttccttcg acagcttcat
      721 tcccggtatg acaggcttac catccaccat tgtggcacca cgacagatgg tccatatgcc
      781 agaaccatca cggtatgccg ttgtgtgatt accctctttt tcgttcagaa actggtcaag
      841 tatctgggga gcagacgcgc ctgcggcaat cagcgccaga acagcagccg acaggccgta
      901 tttgattttt gcgctcatgg atatttatca ggatttatcg gcaacaaata acgagccaac
      961 ttatatacgc cttttaagat aagtcagccc cggctggatc cagtcatcag gctctttctt
     1021 aaagggggag tattgaaatc acgaagaaga gcctcccgca ccgcagcatc catgtctgca
     1081 ccactggcaa gtgcttcaat ctctgccgcc acctgcaaat accccatgca acggccaatg
     1141 cgtttcataa ctctggtgtt ttcgtctttc aggcatctga tagcgagatt aataattttc
     1201 aggcagtgat gatgcgatgc ccggacaggt tttccgttct cataaacgac gaactcaccc
     1261 tcttcacagg aaaccctgaa aggccgatgg ggttcctggt ctttatccgg tgcagacgca
     1321 ataaaatcac ttttatgctc ttcctctccg gcactgttaa ccgtaatgac gtactcaggc
     1381 cgcaggtaag ccgcctcttt ttctgacaat gcggccggta aaaacttttc ctgcaaaaac
     1441 ttcataaaag tctcgtgagc gactttgtgg cagtaatcgt aaatcgccgc ataatgctca
     1501 tcgcggcgtt tgttttcatc ttcagaactc accaacgccg acaatttttt attaagctca
     1561 gtgatttcat tttccaggtg attgaatcgc tgattcattt cttcatgctt cattatttac
     1621 tctccccgtg cagccttacg cttgtcttct cttattttga aatacagatt cgtcagataa
     1681 gtcagaagcc ccagaagcag acttcccagc acaccaatcg cagcccactg tgatggcctg
     1741 acctgatcca accactgcaa aaaccagtag ccggcactgc cggcggaggt gccgtaggca
     1801 atgcccgttg aaattttgtc catggatttc atagcctcac ctccgcaaat aacggatggc
     1861 gtagttttac actgagaaat gaaagggatt tgaaaagaaa aaaccgcaaa agcgggcgaa
     1921 acgatatata caataaggaa agcactctat ccaacaaacc actcacagtt aatcggaata
     1981 aaagcagagt gcttatgaat gatcgcctgc ccgaaggtta gtatttctgc acagcaattt
     2041 tgcaaaaaaa
//
LOCUS       DAJTSA010000245         2032 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000245 DAJTSA010000000
VERSION     DAJTSA010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.247"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..362)
                     /locus_tag="OO369_004247"
     CDS             complement(<1..362)
                     /locus_tag="OO369_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5984268.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /locus_tag="OO369_004248"
     CDS             complement(429..1643)
                     /locus_tag="OO369_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR5984269.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          633 a          493 c          412 g          494 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtggcccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt acgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcgt gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgcctactac catacaagag agaaaaagcg attattcaac cgtgatcaca
     1741 ttaaaaacaa aaatttctac cgaggagaaa aaataccctc aaaaaccacc tgaataatta
     1801 accattattg ttaaaacaac acgttgaata atcacataac aaactccatt aatcattgaa
     1861 aatgagcaca gaaatagaaa ttaattatat tcaattaatt aaaatgtgta ttgcttgatg
     1921 tatttaaaaa tattattttc atggtgagag tggaggtaaa gaagacacaa taataaaaag
     1981 gaatgtgaca aataatggtt ctggttgggc gttaaattta aggtcaccag aa
//
LOCUS       DAJTSA010000246         2031 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000246 DAJTSA010000000
VERSION     DAJTSA010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.248"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1824)
                     /locus_tag="OO369_004249"
     CDS             complement(<1..1824)
                     /locus_tag="OO369_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR5984270.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKIEGHEWVAGNVNGDKGNS
                     LKVNLIGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDARR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRVVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIVSR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGKACPLNEPILLADGTWTTHGNV
                     KIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
                     RRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKF
                     SNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFI
                     PRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELR"
     gene            complement(1932..>2031)
                     /locus_tag="OO369_004250"
     CDS             complement(1932..>2031)
                     /locus_tag="OO369_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5984271.1"
                     /translation="RWNDEKPETIQPQSRPSSAITVSKTGYVFFDR"
BASE COUNT          485 a          580 c          431 g          535 t
ORIGIN      
        1 gcgcagttct tcacttgctg aggaaaaaac aagcgttcca tccttctcga catacccatc
       61 cgtttcaagc agaccacaca gcatgcctat acgcgttgat ttatttgcgg aaaaaaacac
      121 acgagggatg aatttgtttt ttgctgtgca ccccattagt ccataacctc gcagggtctc
      181 catgagtgga tttacctgac cacgcgccgt tgatatcagc cagtccttac catctccaga
      241 gaagttgtaa tcaggcagtt cagccttcat acgctcgatc atgtatggct cgacgtttga
      301 aaacttcacg ctcccattgc taagactacc atccccgaga agggagccga taacccatgc
      361 taatggctct gagtggtcgc caaagtcacc agttatttca ggaatcctaa cgccattttt
      421 gtgcctcttc gtttcactca accgcttcaa cccgaaggta tctatcacgc ggcgtttctc
      481 gcctttcgtg aatccacgac tagtgacctc ccataggtga tcgcctgcgc aatcaacata
      541 acgaccatct tcaaatgtga ctcggtaaac atctctaaca ccctgcggga atatcccagt
      601 gacagttgac ggattgccgt ctactgacgc cacctgatcg ccaattttta cattcccgtg
      661 agtagtccat gtcccatcag ctaaaagaat aggctcattc agtgggcatg cctttccgtg
      721 accgtgcaca ccattgacca gcgtcaactc tgcctcacgg aactggaatt tatctgccag
      781 agattcccac ggtggattaa acagatactg ctgcttgccg tagaaagcgt tgatagtgtc
      841 ctggtaaaac tctcgcgcac tgtagagttc ttcaggatcg aagtaggatg ccgtgccgat
      901 gtactgccag atttcatcct cggtaacacc gttcatcagg cattcgttga tgtctttgta
      961 cggcagagta acaagacggc aacgatgttc accgagtcgg cttacgattt cccttgcggc
     1021 ttcacgacca acatcatcaa cgtccatcga gatgaatatt tcctcaaacc tgtcgaggtt
     1081 gtgatactca aactcaatcc actgttgctt agcgcctttc ccgccaccaa acggcacgga
     1141 taacgccgag atgccgtatt gcgcatagct catacaatca atttcgcctt cgcaaagcac
     1201 aaccaccctc acgccagcgt ccagagcctg ccatccgaac aggcaaggtt cgcaatcacc
     1261 ttctgccata atgactttct tcccgtccgg gcgctcagtg ctgattcgct tgacctgcaa
     1321 caactcacca tcgcgtttgt acggaagcac cagagcatcc agttctcgtt ctccattcca
     1381 caccttgccg ctgacaacct cgtagcgctt tacgacttct ggcgatatgc cacgcgattg
     1441 caggtactca agatgggatt ctgttctggt aacgtagcgg gcgattttct tacgatcagg
     1501 tctggagaat ttcttctcac gtctggcatc gaaatggtga tcgtcatcct tgataccgag
     1561 aaatgctttc gcttcctgca tagcctgatg caggttaatt ccacgacatg ccatccacaa
     1621 atcaagcatg tcaccgccgt ctccctcagc gaaatcagcc cattttttct tgccgataag
     1681 gttgacctta aggctgtttc ccttgtcacc gttgacgtta ccggcaaccc actcatgccc
     1741 ctctatcttg ccgtttggca acaggtgcgg agccaccctg tcaacctgcg cccaaagcag
     1801 gtcgctaagt tcacttggcc tcatgattcc ctcagattga gatttttaaa ccagaaatcg
     1861 acaaacgaaa tacttaacca gccgtggtta taaccagcga ccagtagcga tttgattttt
     1921 gatttcatgg ttcacctgtc gaaaaacaca tagccagttt tcgatacggt gattgcggat
     1981 gatggtctgg attgtggttg aatggtttct ggcttctcgt cgttccagcg c
//
LOCUS       DAJTSA010000247         1985 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000247 DAJTSA010000000
VERSION     DAJTSA010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.249"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(135..1589)
                     /gene="ospC2"
                     /locus_tag="OO369_004251"
     CDS             complement(135..1589)
                     /gene="ospC2"
                     /locus_tag="OO369_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR5984272.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFISNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          624 a          339 c          318 g          704 t
ORIGIN      
        1 tctccaggat tcccggggcg ttcaccacat gcaatgtgtt gttgcagggg aacggtctgc
       61 accatcccct atatcgatca atataacaaa aatgacaacg tcagattcag ggcacaacct
      121 aaatatattt taggtcacct cccaaatcgt ttgcctgata ccgctccatt ttttaataaa
      181 aaatcaattg tttttgaatc tttgcttttc attgcattgt caagcatagt gtccccacta
      241 tttgcttttt gaaatctttt gtttacatca accaatccat tactaataaa atattctagc
      301 actttatagt tggcactatc ttcactaagt aaatactcaa catcatacag ctcacaatac
      361 atattgttgt tcattttctc catctctgcg atatcttgtt ttgtaaaatt aaatttagat
      421 agtaaataaa gtgccatatc agattttgca ttgcctagag aataagcaag agcttgatga
      481 gccatttttt tatcagtcac cttattgtta atttcttcat aattggatgc tttaatggca
      541 tcctccaaag atatatctct gagtcttatc tttttgaagt tatctgtgct tagcattctt
      601 ggtatatgat actcaagctg aaacacaaag tttataattc tatccagact aacaggatca
      661 atgtttttat tataacaaaa ctctcggaat ctttgatctt tactctttct tataaaatca
      721 ataagatgca acccaattcc aagcctcata tctttagtat taaatattgg cacatcagtt
      781 tttccctgat taccatgcac atacctgcta acttcatcca caacctcttt aaagttatct
      841 aagaaaaatg attggtgctc atggtaaaaa acaggtggaa tagcattatc aaagtggtcg
      901 gttaatgtca tatatccata tggagagtca tgatcaatga tatacgcatt agcaccaaaa
      961 tccacagtat gatgtgtttt atttagtggg agtgtttctt gatgattgga aatttcaacc
     1021 ccaaaaaaaa caaaatcgtg gttagctatt tcttcaatat cctcagagaa tgtgttttta
     1081 tatgtttgta tattctcaga cttaatttta tcaaaggact taatattaag agtattattt
     1141 gacaggttag cgttactttg atgtctgaag ttaaaatcag aattaataat aatagagagc
     1201 aagtgacgtt catccgaaga aagaggatga gttttttgta ttatttcaga aagatagtgt
     1261 ctggacacct tatcgaggtg ctcaagtgac cgaaatgatg accgtgctcc agatggcgct
     1321 tcaatagaaa gatttaatga tttgaaactg gtcccttgag agaacatttt actataactt
     1381 tgcgctgcta tagacttcct taaaaagctt ttcacagtat cagcacagtg atctaaatgt
     1441 ttttcactta tgccagaaga ggatgagttt gttacgcatg atatgttttt ttgtaatgct
     1501 tttgtatctt tgttcggatt tatttttcca tcaacaagat caatattgtt ctgaacatta
     1561 atatgattta ctgcttcagg tattttcata aaacatcccc cattatgtgc gtttttatct
     1621 gcagttattt agaataatat tatctaactt aatattttct tgtatgtatt ataacgaaca
     1681 tattttaata tgttgtacga ttttcactct gaaatcccct gagtttaatc acacttcctt
     1741 tactgaaagg aattattgca acagtattat ttgtttttgg atatatatct tgcgccttgt
     1801 tatgtagtta atgctcagaa tatgatagat aaaatagaga cgcaaaacag ggcatgctca
     1861 taacatgatg ataacataaa aaattagtaa caataaatga aagaatcatg ggcgctcaac
     1921 aaaaagaggg taatgctgcc aacttactga tttagtgtat gatggtgatt ttaaggtgct
     1981 tgcgt
//
LOCUS       DAJTSA010000248         1976 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000248 DAJTSA010000000
VERSION     DAJTSA010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.250"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..837)
                     /locus_tag="OO369_004252"
                     /pseudo
     CDS             complement(1..837)
                     /locus_tag="OO369_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          533 a          465 c          457 g          521 t
ORIGIN      
        1 agctgctcta cggtcatatt tttaattgcg ctccggttta caagagccca cccctgtttt
       61 tccagataaa actggaaagt atccagggta cagaccagtg caccgtcagg aacagtttcg
      121 gtgaatctga tattgccgtg ttcgtcgaag tgcacaacca gagtgcgacc gttacccgga
      181 atcatcttgt cagcggacgg ggtattattc tggtgcagtt ctgcctccat gcggtcgaac
      241 atcagcgatg taggcctctt tgaatgcagc ggcttttttc ccagtgaagc ccatcaccag
      301 gaaaacgaag ccgtttttgg tgatttggta cattgggcgt ttttcgcctt tggtgtcggt
      361 gtaggtgacg tcctcaaaat tgagggcgtt aaattcaggt gagcattcag tatttgcgat
      421 ggcacgtaac acattgtcgt gtcgtttgtg gaagaactct gcaaccgcaa cagacgtagt
      481 gacagcgcga ccgttttcga tggttacgtc agggtgagaa agggcaggga tagtagccat
      541 gatggcatcc tcgagtgata agttaattaa ctcaccaccg aggttttcca cgaccataag
      601 ggtggtgaga cgtacagggg tggaaatacc ggtcactcga gaacccggcc agtcttgcga
      661 ctgccctgca catcccacca taatttgaat ggagctgtgc attacgcata aaaaaaccgc
      721 ttcagcgcgg ctatgcgctc gagtaacgtt cgggtttcca cgcccggccc ccgttttatg
      781 aggtgcagat gcactataat tccacccgtt ctggttttca atagctacat tcaacatttt
      841 ctcttacctt tcatcaccga agtgaacttt gttgatgcgg tgcctggtgc ctccaggtga
      901 cgttaaccag ttaacaatta acgccggata cagggaaacc cataaccacc cgatacgtgg
      961 gaactaatcg ctttttaact gttccgcgtg cgctcagccg cattcaccgc atcacaaaat
     1021 tcactttaaa aagggcggac atcagccagc aatgaaactg atgccgccaa aggtaccaat
     1081 caacatggag tgttgtggcg tggttgtcac ttaagcgtat ggtcaacctg acaacccggt
     1141 gtcctcaacg gggaaggaat aactccgcca tacttaccgc cgcgtcattt cgcgttgtgt
     1201 gcctgctttt aactacgtca ggcgaggtgg ttcctgttat tccccaacaa caagaaattt
     1261 gtataatctg aataccccaa caataagaag agtgagcaag tgatgtatta gcgatgtcag
     1321 ctctcaaaga gactgcatca atgttaaaat tattcaatga agcaaaaact caggctgagg
     1381 ttgatcgcgc tgtgtctgaa ataaactcta agttgtcaag cattcagctt gaatatttgt
     1441 catctcattg atgagctgac aatctctaaa agcaatgagg ctgcgctcaa agcaaaactc
     1501 acggagatag aaaagtttaa gttccaaagt gaagactgcg aacctcgcta attgattact
     1561 ggaacgattg tgtatgccag acagtgcaat actagctctg gcaagattac catgtatttt
     1621 tgtccagaat gttttgcgaa gagagcaata tctatattgc aacctttccc tatcgaggaa
     1681 ggcgataaat atacgaaaac tcaatgcccc tcctgtaaaa atgagtatct catggactac
     1741 aatttcaata atgatttttg agtcacggta ttcagatttt taaagaacat gccggatact
     1801 cacccgtgtc cggcgcacgc actccacctc acccgtggag aactccttaa ttaccaacct
     1861 tggcttcgtt ggttagccat taacgctggt atgtaatcat tctggcaatg cttaatttga
     1921 agtggcacac tgaatttggc cacctgaaca gaggtgatat gctcacctca gaacaa
//
LOCUS       DAJTSA010000249         1963 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000249 DAJTSA010000000
VERSION     DAJTSA010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.251"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(58..1209)
                     /locus_tag="OO369_004253"
                     /pseudo
     CDS             complement(58..1209)
                     /locus_tag="OO369_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1274..1922
                     /locus_tag="OO369_004254"
                     /pseudo
     CDS             1274..1922
                     /locus_tag="OO369_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          492 a          495 c          430 g          546 t
ORIGIN      
        1 gtatgacaaa tacaagacga cccatactga tcctacccac gtaatgtgga cacggcccta
       61 agcgagatta tggttttcaa attgttccgg gctgagaccg ccacaggcac tgtgacgacg
      121 ccagcgattg taatcacact cgatataatt aaacaccgtt gcccgcatta tttcccggct
      181 gctaaagcgt tccccgtgga tacattccac cttcagcgaa tgaaagaagc tttccacaca
      241 ggcattgtca taacagtagc ctttcgcact catactgcca cgcaggttgt gtcgcttcag
      301 cattgcctga taatcccctg aacagtattg accaccacgg tccgtatgaa caatgacgct
      361 ttccgggcgt cttctccgcc acaacgccat ttgcagtgca tcacaggcca gttgtgctgt
      421 cattcgcggt gacatcgacc agccaatgac ggcgcgtgac cacaggtcga tgactactgc
      481 gagatacaac cagccctcat cggtacgcaa gtacgtgatg tcacccgccc acttctggtt
      541 tcgggccact ggcgctgaag tcctgctcca gcagattctc caatacgggc aggccatgtg
      601 cacggtagct gaccgggctg aacttccggc tggctttcgc ccgcagcccc tgacgacgca
      661 ggctggcggc aatggtttta atattgaact ccggcagttc gtcagcaagg cggggagcac
      721 cgtatcgctg ctttgcctca aagaatgcct tatggacagc ggcatcgcag gtgagccgaa
      781 actgttggcg caggctcatc tggtgacgac gcctgagcca gacataccag ccgctgcggg
      841 caacccgaag tacacgacac atcgctttga tgctgaactc tgcccgatga ttttcgataa
      901 agacatactt catttcaggc gcttcgcgaa gtatgtcgcg gccttttgga ggatggacag
      961 ttcctcagcc tgctccgcca gttgtcgttt aaggcggaca ttttcagcgg ccagttcgct
     1021 ttcgcgctct gacgaactca tttgttgctg ctgtttactg cgccaggcat aaagctgaga
     1081 ttcatacagg ctgagttcac gggctgcggc ggccacaccg atgcgttcag cgagtttcag
     1141 ggcttcgtta cgaaattcag gcgtatgttg tttacggggc ttcttgctga ttgatactgg
     1201 ttttgtcatg agtcacctct ggttgagagt ttactcactt agtcctgtgt ccactattgg
     1261 tgggtaagat caccctccat taaaactaaa acgggcgctt tctgatatat catctgtaag
     1321 cgcagtaata ccattagtag cacctcgcgc attgattatt gaatattata actggaatgt
     1381 tatatttctt gttatgatgc tgttcagtat tataataatg ttattatcac caaagataac
     1441 tcatgcaaat gagaaagtaa tatgtcaaaa cagtgcatcc tttgtgacca aagagtttat
     1501 ctgcggcact atacttgtct cattaatact cagttctcta ttttgctaca tatcaatatc
     1561 accatcaatt ttcatgcata cttatggcct gagtactttt agttactcaa ttattttctc
     1621 cttctcagtt ttatttttca taatcggaaa tcaattatca aagattgaaa aattatccaa
     1681 cctatggttt ttattaccca atagccaatt ttatcgcatt acaaataaat ggttcaaaaa
     1741 caggatttgc cgcatctatt acaggtttta ctcaaacaat aagcgctggt acaatcagtt
     1801 tcctgtcagt aaccgtaagc gacaacggtg ttggtcccgg aactacttta ggtcttggtt
     1861 cactaataac ttcattactg tcaatagccg taatcgccat tgcaggaaat aataaaaagt
     1921 aagtgtactc tgactgcgta tgtatctact tgaaccgccc cgg
//
LOCUS       DAJTSA010000250         1913 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000250 DAJTSA010000000
VERSION     DAJTSA010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.252"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(53..1702)
                     /locus_tag="OO369_004255"
     CDS             complement(53..1702)
                     /locus_tag="OO369_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5984273.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          452 a          506 c          491 g          464 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accatcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg
     1861 gatttggtgt tgtttttcat tttcagaaca ttcaatcctg ctctcgccgc aat
//
LOCUS       DAJTSA010000251         1912 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000251 DAJTSA010000000
VERSION     DAJTSA010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.253"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            240..509
                     /locus_tag="OO369_004256"
                     /pseudo
     CDS             240..509
                     /locus_tag="OO369_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            506..732
                     /locus_tag="OO369_004257"
                     /pseudo
     CDS             506..732
                     /locus_tag="OO369_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            761..1351
                     /gene="ospG"
                     /locus_tag="OO369_004258"
     CDS             761..1351
                     /gene="ospG"
                     /locus_tag="OO369_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HCR5984274.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            1690..1912
                     /locus_tag="OO369_004259"
                     /pseudo
     CDS             1690..1912
                     /locus_tag="OO369_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          544 a          414 c          449 g          505 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag
       61 tgataggaca ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta
      121 ctcctctgcc atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct
      181 ggtgacgata tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa
      241 tggcacaggt taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg
      301 ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg
      361 ttgaacggca gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg
      421 aatgcggtga tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg
      481 aaatgagcag gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca
      541 ggacaggaag cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg
      601 gacgaacgga ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac
      661 ggtcgtgtgg aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa
      721 aatctcttgt gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat
      781 tattcaaaca ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc
      841 cattctcggt aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga
      901 ttcatctgct ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga
      961 aatggcttgg caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt
     1021 tatacagtat ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag
     1081 tgacattgat acagctgata tccctgataa tatagagagc ctttatctac agttgatatg
     1141 taaattgaat gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga
     1201 taaagaaagt gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc
     1261 tgcaaccaaa aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt
     1321 ttattcgtta ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg
     1381 ctcttctatg agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta
     1441 tgcaccgcca gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa
     1501 ccacccgaaa aaccgccgcg atcgcgtatg ataaatttta accacatgca tacctgtgca
     1561 acaaggcgaa tcacgcacgg atttccagcc agagacacct gaaacgtaac cggctcattt
     1621 aaaccgtctg gtctgttacc tccggttcca caaaaataat gtccatcatt ttgaagtgga
     1681 cactatcgta tggaataccg gacctgaatc actgaagctt tacgccttca tttcgaagaa
     1741 catttaccac gggttgtggc cggatgtcgc ctgggcgtac caaaatcaac tgtctgtggt
     1801 atgttcgtgc gcttccgcaa cgctggcctt tcatggcctc tgcccgcagg tatgtcggag
     1861 caggaacttg atgcccttct ttacggaagt gcctccacag tacctgtcgt ac
//
LOCUS       DAJTSA010000252         1861 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000252 DAJTSA010000000
VERSION     DAJTSA010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.254"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..345)
                     /locus_tag="OO369_004260"
                     /pseudo
     CDS             complement(1..345)
                     /locus_tag="OO369_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(345..1265)
                     /locus_tag="OO369_004261"
     CDS             complement(345..1265)
                     /locus_tag="OO369_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5984275.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1403..>1861
                     /gene="pgrR"
                     /locus_tag="OO369_004262"
     CDS             1403..>1861
                     /gene="pgrR"
                     /locus_tag="OO369_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR5984276.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVLRCTGNSG"
BASE COUNT          466 a          451 c          433 g          511 t
ORIGIN      
        1 acacacaccc agcacgccaa tgcgttcagc atcaacatat ggcagagtta ctgcataatc
       61 gataacacgg ctaatatcct ctacgcgctg tgtcggatct tctatccaac gaggctaccc
      121 accagattct ccctggaaac tggcgtcata agcgagaacg acatagcctt tttcagccaa
      181 tgccttgcca tagatattgc cagacgtctg ctccttacaa ctgccaaaag gatggacgct
      241 gataatcgcc ggatactgtt ttgactcatc aaaatttggt ggaaactgaa tattggcagc
      301 aatatgccag gccatatcac tattgcggat ttgaatatgt ttcattacag agtttcctca
      361 aagaacggag ctaatactga aattgcttca gccacatagg ctttgccgtc ataaagatcc
      421 atatggtttg cgccttcaac aatgtgatag cgtttatcct ggcttgatgc ccgatcgtac
      481 aggtcgtcac tcatccattt gctccctgcc tggctgcccg ccacaatctg catcggctga
      541 gtcaggtaca cttccgccat atggtaagca tcataggtaa taatctggtt aaggctgcgc
      601 aaagtagcgt aacccggtgc tgttggatac tgcgcacgag gtgtgtggta atactcccag
      661 gcctgacgca gttcttcgtt cggcgcatcg gcctccttca ttggagccag tggcataatg
      721 gcgtattctt cgctgctaat atcgctggtt cgggcgtttg agccagcttc aatgtgtggc
      781 agagcatcaa tagatttcac attgttttcc caaccattgc ggaacatcga accaatattg
      841 accgcactaa cggtaccgat ggccttgatg cggcgatcct gaattgcagc attggctgta
      901 tatcctgcac cggcacaaat tcccatcgca ccaattcggg tattgtcgac ataagaaagc
      961 gttgtcaggt aatcaatcac ggcactgacg tcttcagtac gaatgtatgg gttttctaac
     1021 tgacgcggct cgccgccact ttcaccttga taagatgcgt cataagcaat agtgacaaat
     1081 cccttttccg ccagcttttc ggcataggtt ccggccgttt gctctttaac gcccccacct
     1141 gggtgagata ccacaattgc cggatactga cgggtttcat caaattttgg agggaaatag
     1201 atcactgcag acaaagagat ggttggatta ttgctgttag tgaagctgac tttattattc
     1261 atcattcagt tcctcatgga gttgtcggtt cgttttgacg gttggtgata tcactataga
     1321 tattgatcat taagttgatt agaccctaaa tcatgattag acctatcatt taaatgatta
     1381 atagattgta tggagatact gaatgaagcg tgaagaaatc gctgatctga tggcgtttgt
     1441 cgtcgttgca gaggagcgta gcttcactcg cgcagcagcc cgcctgagca tggtgcagtc
     1501 agctttaagc cagatagtgc gccgtataga agaacgattg ggattgcggc ttctgacgcg
     1561 aaccacgcgc agcgttgtac ccactgaagc gggcgagcat cttttgtctg ttcttggccc
     1621 tatattgcat gacatagatt ctgccctgac atccctgagc gatctgcaga accgcccatc
     1681 cgggacaata cgtattacta ctgtagaaca tgcagcaaaa acgatattgt taccagccat
     1741 gcgcacattc ctgaaatcgc atcctgaaat tgatattcag cttaccattg attatggttt
     1801 gaccgatgtc gtttctgaac gttttgatgc aggcgtcctg aggtgtactg gcaatagcgg
     1861 a
//
LOCUS       DAJTSA010000253         1831 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000253 DAJTSA010000000
VERSION     DAJTSA010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.255"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..179)
                     /locus_tag="OO369_004263"
     CDS             complement(<1..179)
                     /locus_tag="OO369_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR5984277.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITH"
     gene            complement(320..874)
                     /locus_tag="OO369_004264"
     CDS             complement(320..874)
                     /locus_tag="OO369_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR5984278.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(871..1161)
                     /locus_tag="OO369_004265"
     CDS             complement(871..1161)
                     /locus_tag="OO369_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR5984279.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1161..1760)
                     /locus_tag="OO369_004266"
     CDS             complement(1161..1760)
                     /locus_tag="OO369_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR5984280.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          445 a          496 c          420 g          470 t
ORIGIN      
        1 ttatgggtga tcggggagag agtttcaaca gagatttgag tggtcataac gataactccg
       61 tacatttgga aattatcgcc accgacgacg ccaatcgaac tggtggcgaa ctgtgcaggg
      121 ttggcgtaac cgggtacgga aaccggcgag cctttcggct cccccacaca gcccgccata
      181 aatcgcgaat gtgactgtgc aaacgatatg aaaaaagacg cgggcgcgtc tcatatcgct
      241 ccgtaaacat ccgggacgcc aatcccgacg ccagattttg ctggcgcgtg aggaatatag
      301 ccccggataa cagattgagt caacaaacgg tttttagatc cccggaagag aatgcatcac
      361 gcatcggtag atagagcata tattcagcaa gatttagcca tacatctacc cgactgcagc
      421 acgtccggaa acaccactca gggtgtgcgt cattcagcaa ttcagccatt ttacgcttag
      481 tcatcccccg cccttcatac cgttgccgga ggacactaat caatccagga tgctctgcca
      541 gcacctcact tatgactcta tcaatacata acgcctctgc atcagtacaa tgcgccagcc
      601 aggtcttttg cttgccattg atcatctctc gcaaaaacgc ttccagctca ggtttctcta
      661 ttcccgcttt tttcatcctg cgcagggctt cattgacggc tgttttcgtc aattttttgg
      721 atgccagcaa ctggttaaac atatttcccg tcttaccgcc gccaatatac gaccagcgcc
      781 cccacatgcg cagttttccc tgaatccaga cactttccag cgtggtgaga cgaaggtgtt
      841 ccccgctttt gcctgtattt gttgggtaaa tcataaataa ccttcctttc tccagatttc
      901 ttgtgtgcga aaaacacctt ctgcatgcat caggcgtaat tcttctttgg tgtaatcgct
      961 ggtttttacc cgcccgtcga ttaaatcgtg gcatgagcta caggcaatcg ctgcctgcat
     1021 atcgtgtggt tttgtcgctg ttccgcacgt ccccgccagc ctgtaatgcg ccagcacaga
     1081 agtttcggga ttgtgattgc agtagccagg aattctgacg gtgcacatct gcccccgcgc
     1141 cgctttacgt aaatccacca ttacgcaaac tccagtaact gcgcggccac attttcgact
     1201 tcctccggag aggaaaattt acggaacagg atccagttcc acagcacatt cagtacagat
     1261 ttataaacct gctgaaactc ggtttcgtcc atgttcgcaa atgcgataga ttttgccctg
     1321 cgcccacgac taccatcagg ataaatatgc tcggtgtaaa atccggcctg aatggttacc
     1381 cactcgcgga aagcgtcaaa cgactttagc aatgccgtat cccgggttct gcatgtcgca
     1441 actgtattaa ggtattgctc tgcggcatca ctcagggctg gagtgtgttc ccgacctact
     1501 gattcgcaca ggtaatcaac gaagcctgat accagttttc gttcgcgagg cgtgatcgcc
     1561 ccaccgaccg gagtccagta atcaaatccc agttgcagga gtttgaaaaa acgcttgtgg
     1621 aatgcgtagt tacgcacacg cttaaagtct gcgtgtatcc actcacctat tttgatttga
     1681 tgcaaaaaat cgcaactctc cggcgtcgcc gggagaagta atccggaaga ggtttgtttg
     1741 accagttgta tatgcgccat cgtagttctc cgctggcgca gtagaatggg tgttcagccc
     1801 gttatgtagt ataccagaat taatgccaat a
//
LOCUS       DAJTSA010000254         1826 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000254 DAJTSA010000000
VERSION     DAJTSA010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.256"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(208..609)
                     /locus_tag="OO369_004267"
                     /pseudo
     CDS             complement(208..609)
                     /locus_tag="OO369_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1038..1826
                     /gene="virF"
                     /locus_tag="OO369_004268"
     CDS             1038..1826
                     /gene="virF"
                     /locus_tag="OO369_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR5984281.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          570 a          330 c          340 g          586 t
ORIGIN      
        1 caagggaata tagtttgtgt aagcatattg cttccttatg ttatatttta gtaaagaatt
       61 tattttttac attatagtcg gctaaatgaa gtttactgta aaaaaacgat acttttttcg
      121 taagcgatga gatgtggagc cagaaaaatg gctgcgttac gtcattgaac tgtccttgac
      181 ccacgatatt cagacagtcg ttgtatctta agttgacatt acccgctgac gtcgatattc
      241 ccttggtgag agataaccca acgcactatg cggatggttt tcattgtaat gctcgatcgc
      301 cactgcaagg ttatgcaatg ccgttcttgg tttcggcatg aacgcgatgc agtcttcctt
      361 catcgttttc atgaaccgtt ctgctattcc attactctga gggctactga ctgccgtcgt
      421 gcagggctcc agattcaact ctctggcgaa ctgccgtgtt tcatgagccg tataagccga
      481 accgttatcg gccactggat tggccgttcc ggtcctttat cgccatatcg ctcctgacat
      541 gacatcctgc accgttgctt tgtcataatc tcccgtgcct gctgcccagt ctatggcttc
      601 acgatcgcaa tatggaacgt aacgctgtca tcgggagaat cgtcaatgcg gcattcggtt
      661 aacgcttacc aatatcaaca ccaaccggat ttgtggagcc tccagtctga aggggcttta
      721 tgcgttccgt ataggatatt atgatgctgg agttttgcga agctccactc ctttccgaac
      781 atttacacag atattgccta agaaaagtga aaccataata tggttatagt ccctttcagt
      841 gcaaatactt agcttgttgc acagagaaat agaagctgca taagctcttt cttcaaaaaa
      901 tgtaaataaa gttaaatata ggaaaaatta cttaatctat cttaataacg gaaagatttt
      961 gtatacaatc actgttacag aaatttctta gttactctgt aaacactaaa tatagtttgg
     1021 tatattctgt tgaatttatg atggatatgg gacataaaaa caaaatagat ataaaggttc
     1081 gcttgcataa ctatattatt ttatatgcaa aaaggtgttc aatgacggtt agctcaggca
     1141 atgaaacttt gactatcgat gaagggcaaa ttgcttttat agagcgaaat atacaaataa
     1201 acgtctccat aaaaaaatct gatagcatta atccatttga gattataagc cttgacagaa
     1261 atttattatt aagcattatt agaataatgg aaccaattta ttcatttcaa cactcctatt
     1321 ctgaggagaa aagggggtta aacaaaaaaa tattcctcct ctctgaggag gaggtttcta
     1381 tcgatttgtt caaatctata aaagagatgc ctttcggcaa aagaaagatc tatagtttag
     1441 cttgcctttt atcagctgtt tctgatgagg aagctttata tacttcgata tcgatagctt
     1501 cttctcttag tttttctgat cagataagga agattgttga aaaaaacatc gagaagagat
     1561 ggcgtctttc tgatatttca aataacttga atttatcaga aatagctgtt agaaaacgat
     1621 tggagagtga aaaattaaca tttcaacaaa tccttcttga tattcgcatg catcatgcag
     1681 caaagctttt attgaatagt caaagctata ttaatgatgt atcaagactt atcggaatat
     1741 caagcccatc ttattttata aggaaattta atgaatatta tggtataact ccaaagaaat
     1801 tttacttata tcataaaaaa ttttaa
//
LOCUS       DAJTSA010000255         1809 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000255 DAJTSA010000000
VERSION     DAJTSA010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.257"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            173..1198
                     /locus_tag="OO369_004269"
     CDS             173..1198
                     /locus_tag="OO369_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5984282.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1809
                     /locus_tag="OO369_004270"
                     /pseudo
     CDS             1266..1809
                     /locus_tag="OO369_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          421 c          462 g          505 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctggct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtgag
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgcc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgttt
//
LOCUS       DAJTSA010000256         1776 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000256 DAJTSA010000000
VERSION     DAJTSA010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.258"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            457..>1776
                     /locus_tag="OO369_004271"
     CDS             457..>1776
                     /locus_tag="OO369_004271"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5984283.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLED"
BASE COUNT          463 a          433 c          425 g          455 t
ORIGIN      
        1 ttgaaacaaa tgacggtaga gaatgttaac actgccgtta aacaatatct ttctcatccg
       61 gtaaatactt ataccggagt attattgcca aaataataac agccccgtca acacatcgtt
      121 ggcggggatt ttagcaatat tcgctatttt tatgtaataa ttttataaat gcgttcaaaa
      181 taataaccaa gtactaatag tgatatttta atacctgatt tttacgtgat aattcaggag
      241 gcacagaaag cgcataaaaa taacaacata aaacacctta ccaccaccca agaattctct
      301 attacattgt ttttaatgaa aaaatatcat tccaacaata tctcacgata aataacatta
      361 ggattttgtt atttaaacac gagtcctttg cacttgctta ctttatcgat aaatcctact
      421 tttttaatgc gatccaatca ttttaaggag tttaaaatgg ataagaagca agtaacggat
      481 ttaaggtcgg aactactcga ttcacgtttt ggtgcgaagt ctatttccac tatcgcagaa
      541 tcaaaacgtt ttccgctgca cgaaatgcgc gacgatgtcg catttcagat tatcaatgat
      601 gaattatatc ttgatggcaa cgctcgtcag aacctggcca ctttctgcca gacctgggac
      661 gacgaaaacg tccacaagtt gatggattta tccattaaca aaaactggat cgacaaagaa
      721 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa acatggttgc cgatctgtgg
      781 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      841 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      901 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tgcaaatctg ctggcataaa
      961 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
     1021 atggacccga aacgcatgat tgaagcctgc gacgaaaata ccatcggcgt ggtgccgact
     1081 ttcggcgtga cctacaccgg taactatgag ttcccgcaac cgctgcacga tgcactggat
     1141 aaattccagg ccgacaccgg tatcgacatc gacatgcaca tcgacgccgc cagcggtggc
     1201 tttctggcac cgttcgtcgc cccggatatc gtctgggact tccgcctgcc gcgtgtgaaa
     1261 tcgatcagtg cttcaggcca taaattcggt ctggctccgc tgggctgcgg ctgggttatc
     1321 tggcgtgacg aagaagcgct gccgcaggaa ctggtgttca acgttgacta cctcggtggt
     1381 cagattggta cttttgccat caacttctcc cgcccggcgg gtcaggtgat tgcacagtac
     1441 tatgaattcc tgcgccttgg tcgtgaaggc tataccaaag tacagaatgc ttcctaccag
     1501 gtcgctgcct atctggcgga tgagatcgcc aaactggggc cgtatgagtt catctgtacc
     1561 ggtcgcccgg acgaaggcat cccggcggtt tgcttcaaac tgaaagatgg tgaagatccg
     1621 ggatacaccc tgtacgacct ctctgaacgt ctgcgtctgc gcggctggca ggttccggcc
     1681 ttcactctcg gcggtgaagc caccgacatc gtggtgatgc gcattatgtg tcgtcgcggc
     1741 ttcgaaatgg actttgctga actgttgctg gaagac
//
LOCUS       DAJTSA010000257         1759 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000257 DAJTSA010000000
VERSION     DAJTSA010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.259"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..263)
                     /locus_tag="OO369_004272"
                     /pseudo
     CDS             complement(1..263)
                     /locus_tag="OO369_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(378..1016)
                     /gene="ospI"
                     /locus_tag="OO369_004273"
     CDS             complement(378..1016)
                     /gene="ospI"
                     /locus_tag="OO369_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR5984284.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1175..1645)
                     /locus_tag="OO369_004274"
                     /pseudo
     CDS             complement(1175..1645)
                     /locus_tag="OO369_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          502 a          433 c          319 g          505 t
ORIGIN      
        1 agcaagcgct gtaactcttt tatttgtttt atagcggcag ccagttctga ggcaggaacg
       61 acatcctctc cggcagcaac agcagtgaga cttccttcca gatattattt tttccactta
      121 agcaacaggc tgggctgaat accatgcaaa cgcgccacat gggacaccgt catgccaggc
      181 tccattgtct gctgaataat ggcaattttt tcctgaggag ttttacgttt acgaagctcc
      241 agccccagca aagttccagt catctcaaaa ttagaactag tgttagacat atatccaagc
      301 ctatctctta ttttaagata catccactgt ctggcgtttc aaggggccgg ctcaaatcta
      361 tgtacaggct ctcagcatca gcaaagcctc ttacttttcc aactactact gttagcatag
      421 tgatcaaatt cctgatcaga tacatgaatt acctgaaccg aactattttc atctacaaat
      481 gcatctggcg atttttctac aggatcataa agattaaatc cctttcctgt ttgaacatcc
      541 aaaaacataa ttttttcacc atcgttaaat ccagctatcc aatgagtggc ctgttcaata
      601 gaaatcatta ctacacttcc ttttgaagta ttttgtaaaa tattttctgc aatacgctca
      661 cgaaatttac tataagtttc attaggatgt gacactatgc cttcactatc aatttgctct
      721 aaatgcctat gaaaaaatcc ggtcatatac tctgatattt cattaggtac gggagcctct
      781 cttactccat accctaacat agccatacaa gcatgaagcg cacaccctga acagttacca
      841 tccccaccag acggatttat cattttctgt aaagtcgctg ccaattcaga atctttatcc
      901 atatcaccaa gacttttaga gtttaaagat tccagcaatt ttttaacatt tacatttccc
      961 tcctctgtat gtgcctcata tccagcagtt ccctgtaacg acaccccatt aatcatattg
     1021 tatcctgaca gtatatatac tcactatatt ttcttcataa caaatatccc tctgatatga
     1081 caacaaaaaa cggagccgga ttcctgtttt tgtgaactgc agctattcgt gtctaaaaag
     1141 tgatccactt accaccaacg gcctaatata tacagaacgg agtaaacagg actaacagtt
     1201 cccgacaggt ctcatcattc cgtggcccga aagtgtaagc caacacaccg ccagttttag
     1261 tgttgtacga ataccagagc cagtgttgtt gcagccttgc tgccaacgaa gcgccactgc
     1321 tcatcaagtt cacagataag ggcaacatca gcgtgagcga ctggcgcaga tgttatccgc
     1381 cggggcggga gctttttaat gtccgtatga cggtattgat gccgattttc agtgtcctgg
     1441 cggtatcacg gccccctgcg ccgttatgtg ccatttcaac gatctgctcc ttaacgccgg
     1501 gttttctggc ttcataagag taagtgagct ggaaaacacg tttacatgag cgacaacgga
     1561 aacgctcatg ccgggaacaa ctacggccat gtcgataaac ttcatcggaa tgacagcgcg
     1621 gacaatgaac agtaacggta gccatggaaa accttaaata ccagtagatt accagaaact
     1681 tctgtcgatt gaagacatca cccgaagttt gaattcattc gaatactttt tacgaggttc
     1741 ttttcgccag tcctgtaat
//
LOCUS       DAJTSA010000258         1749 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000258 DAJTSA010000000
VERSION     DAJTSA010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.260"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            454..1302
                     /locus_tag="OO369_004275"
     CDS             454..1302
                     /locus_tag="OO369_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5984285.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATI
                     LTLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTD
                     AKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMI
                     PGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVE
                     QLLSLK"
     gene            1319..1600
                     /locus_tag="OO369_004276"
                     /pseudo
     CDS             1319..1600
                     /locus_tag="OO369_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          500 a          398 c          411 g          440 t
ORIGIN      
        1 agcattatat agttctactc tggcgagata cgttggcata ttcatttcct tactggttgt
       61 gtgagaactt cagtaaagat accaccaaag cccggaagtg gtgaaataaa accgggcaca
      121 acacgaaggc gcatttccgg tatccataaa gagtcggtct tgtctgtgaa atttaaatgg
      181 tgggagtgcg cttccggttg taaataacga cattgctgtg tgaagtacca gttggcggca
      241 tcggtttaat tgctggctga tgtccgccct ttttaaagtg aattttgtga tgcggtgaat
      301 gcggctatgc gcacgcggca cagttaaaag tcatgttagt ccttattggt ttgggtggga
      361 aagccaactg taattgttaa ctggttaacg tcacctggag gcaccaggca ccgcatcaac
      421 aaagttcact tcggtgatga aaggaaagag aaaatgttga atgtagctat tgaaaaccag
      481 aacgggtgga attatagtgc acctgcacct cataaaacgg gtgccgggat tgctaccccg
      541 acgatgacta ctgcgcataa ccgcgctcag gcggtttttt tatgcgtcaa gcacagccac
      601 attcagatta tggtggggcg tgcagggcag ccgcaaggct ggccggtttc ggtagtcacc
      661 ggttgtagca accctgtgcg tctcaccacc catgagattg ctacctctgg tggtgagtca
      721 tttaaactga ctatcgaggc tgccatcatg gctactatcc ttaccctttc tcaccctgac
      781 gcaaccatcg aaaatggtcg cgctgtcact acgtctgttg cggttgcgga gttcttccgg
      841 aaaatgcaca aaaacgttat tcagaagatc gaaactctcg aatgctcgcc ggagtttaac
      901 cggcttaatt ttaagcccgt cacctatacc gacgccaaag gcgaaaaacg cccaatgtac
      961 caaatcacca aaaacggctt cgttttcctg gtgatgggct tcactggcaa aaaagccgcc
     1021 gcattcaagg aagcctacat tgctgagttc gaccgcatgg aggccgaact gcgccagaat
     1081 aacaccccac ctgcagacaa gatgattccg ggtgatggtc gcactctggt tgttcacttc
     1141 gacaaattcg gcaatgtcga attcaccgaa accgttcctg atggcgcact ggtctgtacc
     1201 ctggagactt tccggtttta tctggaaaaa cagggatgga ctcttgttaa ccggggcgca
     1261 attaaaaata tgactgtgga acaattactt tctttaaaat aaaaatggag ctacggatat
     1321 gaaccctatg cttgattatt gttttacctc tgcacctcag cagaccataa cacacaccgg
     1381 aatcaaaaat ttgttatgga taatcaaaaa aaacctccag tagaaaggtg atcccgattt
     1441 aattccctgt gcttatggcc tgtgtgacag acaagtaagc cctgataagg cttacactgc
     1501 tgaacttatt tacatgcacg gaaccatgat gaggaaaaag aaacgcaaat attgtagtga
     1561 aatctgcgcc gaaaaagacc agatggcaca cgaactttaa ttaactgact attcgaaact
     1621 gaatttatgc cagcaatggc agggattcgc tcaaccttaa ttaaggagaa aaacatgaac
     1681 accaattatg aagccactgt tgcaactact gataacattg ttcacgaggt ttacctggaa
     1741 ggaaagcat
//
LOCUS       DAJTSA010000259         1717 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000259 DAJTSA010000000
VERSION     DAJTSA010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.261"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(110..577)
                     /locus_tag="OO369_004277"
     CDS             complement(110..577)
                     /locus_tag="OO369_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5984286.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..882)
                     /locus_tag="OO369_004278"
     CDS             complement(643..882)
                     /locus_tag="OO369_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCR5984287.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1017..1622)
                     /locus_tag="OO369_004279"
                     /pseudo
     CDS             complement(1017..1622)
                     /locus_tag="OO369_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT          550 a          378 c          305 g          484 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tgggtttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat
      901 tatttaaatt caaaccacca gcttcatctg cgtgtcaccg gtatcccata tttttgggat
      961 gtggctataa ttatctattc ccgctattta gtcttcaaaa aaaattacat ttaatatcat
     1021 ttatcttgat taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct
     1081 aacacggtaa gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt
     1141 ttgtcagtat gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc
     1201 tcaggaatgt ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta
     1261 ctgtcttcat cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat
     1321 ctgacgcctt ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat
     1381 gcctttctaa gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc
     1441 tcccgccaaa cctcattgat aatatgttgc ttatcggatt ctccctgtag taaaaggagt
     1501 aaaaatcgat agccgttttc agacagattg attacattgc ctgtactggt ggtgatacgt
     1561 cgagacgctg ggtaaaacta aactccctgc cctaaagaaa aataaaaacg gtcatcgctc
     1621 ataagtaccg acccctgttt tcttcttcac atgactactc attacaactt tgtatgaagt
     1681 aagatgcaaa atcatattgt ttacattaag ttaagca
//
LOCUS       DAJTSA010000260         1660 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000260 DAJTSA010000000
VERSION     DAJTSA010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.262"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(62..138)
                     /locus_tag="OO369_004280"
     tRNA            complement(62..138)
                     /locus_tag="OO369_004280"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(102..104),aa:Arg,seq:tct)
     gene            complement(140..215)
                     /locus_tag="OO369_004281"
     tRNA            complement(140..215)
                     /locus_tag="OO369_004281"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(180..182),aa:Ile,seq:cat)
     gene            complement(392..1081)
                     /locus_tag="OO369_004282"
     CDS             complement(392..1081)
                     /locus_tag="OO369_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5984288.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
     gene            complement(1078..1437)
                     /locus_tag="OO369_004283"
     CDS             complement(1078..1437)
                     /locus_tag="OO369_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5984289.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDETNIVRGL
                     PVPGGRLGIKITELECA"
     gene            complement(1437..1574)
                     /locus_tag="OO369_004284"
     CDS             complement(1437..1574)
                     /locus_tag="OO369_004284"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984290.1"
                     /translation="MVPALRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVL
                     R"
BASE COUNT          404 a          465 c          353 g          438 t
ORIGIN      
        1 atcgaactgg tgacctactg attacaagtc agttgctctg cctgctgagc taagtcggcg
       61 ctggcccacc accgaggact cgaacctcgg accgtcaact tagaaggttg atgctctatc
      121 cggatgagct agtggtggtt ggtggccctt gctggacttg aaccagcgac ctggcgatta
      181 tgagtcgctc gctctcacca ctgagctaaa gggccggagg cagaataata accatatgtc
      241 atcacatctg caaactcatc tgaccaccag cacgtttaac gtcctgtgcc atttttcagg
      301 cataaaaaaa ccgctcggcg gcgggttttg cttactttac catcgcgtac aaaatcggca
      361 aagtatcaga tttgcatgag atatatgcct tttaatctac ttttgcaata ctttgctttg
      421 aaaatgccgc tttttgttct gaacgtgttc tcatcacaaa caataaagcc tcactatcca
      481 gtcggtgaaa aatgtgtttc attacaaccc agtgaccagt aaatgttttg gaccagtttt
      541 tggttgttac tcccgccagt aatgccagct cctgatattc ataaccttcc ccaccaaaaa
      601 gttctgcttt taccgcctgc gccgccagcc agattaattt tttcaggcgt tcctgtgttt
      661 tctctgcaat tttcctagtg ccagaatgag ccttaaactc ttcccacgcc cagtgggtta
      721 tggtgatctg atactcccag caaatacttc cgctgtaaca ccacaacaac caggctttat
      781 agtgctcttc aagagacaga acagcccgcc gccacgatga tgtcgaaaac gcaacctgac
      841 tgaccagagg aattgacgcc cccttcgcca gtgattgttt tcccgggatt ggtggattat
      901 cccgcgttat catttttccg gtcacctcat cgcggtaccg gattttttta cgcctgtaac
      961 gccctgtatc gaacatggca ttctcctgcc aggcttcaag ctgacctttt gtcgccccac
     1021 tcaaatcagc ggtggcgata atgagctgct cacgaacaaa ctgtaaatac tggttattca
     1081 tgcacactcc agctctgtga tttttatccc caaccgacca ccaggaacag gcagtccgcg
     1141 cacaatattg gtttcatcaa actgctcgtc gtcgataagc aaccccgcat gtgtcagtgc
     1201 atccagtggt gctttcagga tattgtccag gtcacgacgc cgcttatccg gtggctctgc
     1261 aataatcttt atcgccagtc ttccggacag gcttaatttc agtcgctgct gacggacaat
     1321 aagcgccaca tcccggcgat aacgctcacc ggcttttgat acaaaatatg tgctgccacg
     1381 acgtcgccag taagtattca ccgtcggcgg ataaggtaaa ataaactcat ggcgcatcag
     1441 cgcagcacct cctgcaccag cttttcaaac tttccgactc tggtttccag ctctgccaca
     1501 cagtccacca gctcatctac tgctttctgt gcgcgatgct tcgcctgcat cagttcccga
     1561 agcgcgggta ccatattgag gtagcctgag tttaacggac actccttcct gaaatagaat
     1621 ggcatcagaa ggagccaata atgagcagaa aaacccaacg
//
LOCUS       DAJTSA010000261         1655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000261 DAJTSA010000000
VERSION     DAJTSA010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.263"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(156..1391)
                     /locus_tag="OO369_004285"
     CDS             complement(156..1391)
                     /locus_tag="OO369_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5984291.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="OO369_004286"
     tRNA            complement(1538..1614)
                     /locus_tag="OO369_004286"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
BASE COUNT          420 a          450 c          394 g          391 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtc
//
LOCUS       DAJTSA010000262         1634 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000262 DAJTSA010000000
VERSION     DAJTSA010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.264"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..854
                     /gene="spa40"
                     /locus_tag="OO369_004287"
                     /gene_synonym="spaS"
     CDS             <1..854
                     /gene="spa40"
                     /locus_tag="OO369_004287"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR5984292.1"
                     /translation="NDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKFV
                     LATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIFS
                     QVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKREY
                     IEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPFI
                     SLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVWL
                     EQVENTH"
     gene            867..1109
                     /locus_tag="OO369_004288"
     CDS             867..1109
                     /locus_tag="OO369_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984293.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT          531 a          221 c          300 g          582 t
ORIGIN      
        1 ttaatgactt cgaaattaat gagggtaaat acttttttgc agtggttatt gtctttttta
       61 agataattgg cttcccactt tttttctgtg ttctttcggc tgtgttgcca acattggttc
      121 aaacaaagtt tgttcttgcg actaaagcta tcaagattga tttttcagta ttaaaccctg
      181 ttaaagggtt aaaaaaaata tttagtataa agacaataaa agaatttttc aaaagcattc
      241 tgcttcttat tattctagca ttaacaacct atttcttttg gattaatgac cgaaaaataa
      301 ttttttctca ggtgttttct agtgttgatg gcttatatct tatttggggg aggctgttta
      361 aggatataat attatttttc ttggcatttt ctattcttgt tattattctt gactttgtga
      421 ttgagttcat tttatacatg aaagatatga tgatggataa acaggagata aaaagagaat
      481 atatagagca agagggacac tttgagacaa agtcgagaag gcgtgagttg catatcgaga
      541 ttctttcaga gcagactaaa tctgatatac gtaattcaaa attagtggta atgaacccga
      601 ctcatattgc aattggtatt tattttaatc cagaaatagc gcctgcacct tttatttctc
      661 tcattgaaac taaccagtgt gccttggctg tcagaaaata tgcaaatgaa gttggtatac
      721 cgactgtgcg tgatgtgaaa ttagctagaa aactatataa aacacataca aaatatagtt
      781 ttgttgattt tgaacacttg gatgaagtcc tacgtcttat tgtttggctt gagcaggttg
      841 aaaacactca ttaataagta aaggagatgt gttatatggg agttaatttc tgtaataaaa
      901 taggtattga tcagtctgaa tttgaaatag aatcttccat cattaactcc attgctaatg
      961 aggtattgaa cccaatatct ttccttagca ataaggatat aataaatgtt ttactcagaa
     1021 agatttcatc tgaatgtgac ctggttagaa aagacattta tcgttgtgct ctggagttgg
     1081 tcgttgaaaa aacacctgat gatttataat cattcataat aactcttttg ctgatataaa
     1141 tatttttgtt gaagatattt atgtagtcac aggatcaatt gtagaaaatt gttatgtttt
     1201 tgttaggaac tgcactaaat aatatatgat aagacagcaa aaacgactga ccataatcct
     1261 tcttttatta ggagttgata agagagacta ttcatcctgt aatgttaaaa ctctgttata
     1321 tagtattcgc gattatgcaa agtctgtaaa tgatcatgag atactaaccg agagtaatcg
     1381 tttattatct cattgcattt cagatagtaa cggagctttc tttaaaagta gtaaatacgt
     1441 tccccttaag tacttacgta aaaggagaat agcacgaaag ataccaaatg attagcactt
     1501 tgctagtaag cttgctggta aactatattg accgttgggt ttgttttgag actgtaattt
     1561 aaattgtgta attgcctgtt tttgatatgt ttactccaac aacggagaca ggtaaattat
     1621 ggacgaaaag aagc
//
LOCUS       DAJTSA010000263         1633 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000263 DAJTSA010000000
VERSION     DAJTSA010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.265"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..291
                     /locus_tag="OO369_004289"
                     /pseudo
     CDS             1..291
                     /locus_tag="OO369_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            293..659
                     /locus_tag="OO369_004290"
                     /pseudo
     CDS             293..659
                     /locus_tag="OO369_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            587..1060
                     /locus_tag="OO369_004291"
                     /pseudo
     CDS             587..1060
                     /locus_tag="OO369_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
BASE COUNT          406 a          394 c          481 g          352 t
ORIGIN      
        1 tcaacgtatt accactgtca gcaacagcga catcatcatg ataaacgcag tgcccgtgcg
       61 cagcgcgatg actggctgaa gaaagagata ctgcgcgtat acgatgagaa tcatcaggta
      121 tacgctgtgc gtaaagtctg gcatcagttg ttacgggaag gtatcagagt ggccagatgc
      181 actgtggcac gtctcatggc ggttatgggg cttgccggtg ttctccgggg taaaaaggtc
      241 cacactaccg tcagccggaa agccgttgcc gcaggcgacc gcgtaaaccg tcatcaaggc
      301 aatgtgccgc gtactccggg tggcccgcag cggctggtat acgtggtgtc agcggcggac
      361 aaggataaac acacgtccgc agttccgtca gcactgcgac agcgttgtcc gcaaggcttt
      421 tacccagtcc agcgttacgg tgccccacgc ctgacggatg aactgtgtgc tctggttacc
      481 actttaacgt aaaaaccgtg gcagccagtc tgcgccgtca ggaactgagc gcaaaagcct
      541 cccagaagtt cagcccgatc agttaccgtg cacacggcct gcctgtatca gaaaatctgc
      601 tgacgcagga tttttacgcc agtggcccga accagaagtg ggcgggagac atcacgtact
      661 attacagctc accgactgcg gggaaacacg gggcgccggg ttattgaagg tgacctgtcc
      721 agctacttcg acacagtaca tcaccggctg ctgatgaaag ccgtctgccg caggatcagt
      781 gatacacgtt ttatgagact gctgtggaaa acgccgtgtt gatgttggtc tcttccgggc
      841 ggtcagtgaa agtatgccac agggcggtgt gatctcgccg ttgctgtcga acatcatact
      901 gaatgagttc gaccagtatc tgaataaacg ctacctgagt ggaaaagcca gaaaggatcg
      961 ctggtactgg aatcacagta tccagcgagg ccgaagtact gctgtcaaag aaaactggca
     1021 atggaaacct gccgttgctt actgttgcta tgccgatgac tgtgtgccac gaaggcgcgt
     1081 cctggggacg tgagtccctg tgcagccatg cagcacaaga gatgagggaa ggccccttgg
     1141 tgtcggcgtg caggcgcagg caccaaacca tccactgctg ctgcatttaa gagatgtggt
     1201 ggtgagcgag atgggaaaag gtcggacgtg agccgatcag gtatggttcg aagagacgaa
     1261 cgaaagtgaa ccgctgatga tgtgtcgaaa gcatatagat gatgtcaaaa caatggtgga
     1321 gtcgttcctg tgggatatgt ttactgcctg cgcggcatcc ggcataaaag cggcgtgaag
     1381 tcgattcagg ctcttgtggg gaacgtggac ctgtcacccc gatgtgaagg gagaagcgca
     1441 agtggaagcc ccatgagcgc cagagtacca atgcggggta cagggacgga gcaccaagcg
     1501 attgcgcagt aaacatgctt gacaacttaa ttggggcccc ctcttccttt tatggtgtaa
     1561 ttgtttgatt gaatacttcc ggggatttca gtatgaaatg aagtatattt aatatacttt
     1621 acaaaaagtt tgt
//
LOCUS       DAJTSA010000264         1625 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000264 DAJTSA010000000
VERSION     DAJTSA010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.266"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..184)
                     /locus_tag="OO369_004292"
                     /pseudo
     CDS             complement(1..184)
                     /locus_tag="OO369_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            264..1457
                     /locus_tag="OO369_004293"
     CDS             264..1457
                     /locus_tag="OO369_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HCR5984294.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
     gene            complement(1477..1625)
                     /locus_tag="OO369_004294"
                     /pseudo
     CDS             complement(1477..1625)
                     /locus_tag="OO369_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          455 a          361 c          390 g          419 t
ORIGIN      
        1 ttgtttcatg ctttcgttgg ttagcgacat cgtgcagcca gcgcagaccg tggcggttgg
       61 ctgtttcaac atcgacagtg ctccccatcg ggcgcaggcg agtcattagt gggatgtaaa
      121 aactgttacg ggtgtactgc accatccgtt ccaccttacc tttagtctgt gccctgaagg
      181 gctgaggaat ccccagaaaa cgagacaaac atagcattat gtgatctact gatttgtgcc
      241 aacaattcaa tgagatcact gctatgcctg ttcataaagt atgtcagaat ttcttccggg
      301 atgccttagc accatttcat cagtaccgac aaaatgccct gatggatgcc acaatggcgt
      361 taatcaacgg agcatctctg acgcagacca gtatcggacg ttttttgccg ggtaatgcac
      421 aagttaagaa taagatcaaa cgtattgatc gcctcatggg taatgaggcg ctccatcgcg
      481 atattccaat gattttcagg aatatcactt caatgctgac acggcagtta tctttatgtg
      541 taatagccgt agactggagt ggctatccct cccaggagca tcatgttctt cgtgccagcc
      601 ttctgtgtga cgggcgttcc attccgttat tgagtaaggt tgttccttct gaaaaacaaa
      661 ataatccatt gatacaacat gatttccttg attcccttgc tcaatccctg ccccccgatg
      721 ccagggttat tattgtcacg gatgctggat ttcagagcgc atggtttcac catattactt
      781 ctctgggctg ggattttatt ggtcggatca gaaataacgt tcagtattgc ctcgataacg
      841 cgcctgaaag atggttaaaa gtgtcggata gccctgaatg taaaacaccg gaatatatgg
      901 gggctggaag gctggttaaa gaaaggaaaa aaagcatccg ggggcatttt tacacctata
      961 agaaatcggc aaaagggcga aaaaagaaac gttcgaaagg tcagagtggt ttaaacaaaa
     1021 cagacaaaga acagagcaaa tcagcaaaag aagcctggtt gatcttcagc agtacaaatg
     1081 acttcagagc gcgagaaatc atcaagttat acagtcggcg aatgcagata gagcaaaact
     1141 ttcgtgatga aaagaacggg cgcttcggat ttggtcttcg ggccagcaaa agtcgttcaa
     1201 caggaagaat tctggttctg agtctgctgg caacgttgag cactatcgta atgtggttac
     1261 tcggttatca cgctgaaaat aaagggttac acctgaaata tcaggctaac agcattaaat
     1321 cccggcgggt tatctcatat ctgacgttag cgaagaatgt tcttcgacac tcgccactta
     1381 ttttaagacg aacagtactg agcacagttc taaaccatct gtccagaacc taccgaaata
     1441 tggtgctggt ttattagcag taattttgtg gggatccctc agcctgaagg ggcgacacag
     1501 tcggggagag aagcccatct ccttgccgaa ctgccacagc gaaggatgga accggtgctg
     1561 accggtctga tatgcgtcac gttgcagaac cacagttttc atattgtcat acaacacttc
     1621 gcgcg
//
LOCUS       DAJTSA010000265         1602 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000265 DAJTSA010000000
VERSION     DAJTSA010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.267"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(334..480)
                     /locus_tag="OO369_004295"
                     /pseudo
     CDS             complement(334..480)
                     /locus_tag="OO369_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            534..716
                     /locus_tag="OO369_004296"
     CDS             534..716
                     /locus_tag="OO369_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HCR5984295.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(817..1146)
                     /locus_tag="OO369_004297"
     CDS             complement(817..1146)
                     /locus_tag="OO369_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCR5984296.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1318..>1602)
                     /locus_tag="OO369_004298"
     CDS             complement(1318..>1602)
                     /locus_tag="OO369_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984297.1"
                     /translation="HFVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          435 a          369 c          377 g          421 t
ORIGIN      
        1 tagcaatgtc tgtcgcacaa caaccacaat gtcacaaata aaaaatgaga ataaccagtt
       61 ccctgaaggc gacgtagccc ggtacaagag taacccacgt tagcgcttgg cgacggcgga
      121 acaaagctga ttcgcgacgt aagaaagtgg caggctcata tgacggacct ctgtaatgcg
      181 gtatccaacc cgcgaatatc agcatgattc accgtcggat ttactgccac gtcgccctca
      241 gggaattaaa cctaaaaaga aaatcgtcaa aattagagat tccggtttga cacggaaagt
      301 catatcagcg tcaacggagc accgtattga cgcttattta ttggtgagaa ctacgttcca
      361 tggcaggagt tcgtcaacac ggttggaggg ccattcaggc agtatgctca gaatatggcg
      421 cagatacgct tccggatcga taccgttcag acggcaggtg cctgcttttc cggcaacaag
      481 caggaatctc agcgctgaag caggaattag aggtaaaaac gccctaccgg gccatgaatc
      541 acccggtcat tggagtagta accaaagcag atttagccag catggaacaa atatctttgg
      601 tgaaaagttg gttacgggaa gctggagcgc acaacgtgtt agtaaccagt gcagttaata
      661 acaatggagt tacagaactt tttgccttgc tgcatacaga agaaggctgt tgttaatttc
      721 gattggttct cagatgagaa tgatgacggt gatacacaga taagaatcaa tgagcaatga
      781 gggttgccgg gcaaccctca ttgaataaaa cgggaattac ttcgcttcgc cgtttttcat
      841 ttcgcccata gctttcagct ttttggagat atcgcggcgt tctttggaga gctcggcatt
      901 tttgatgatg taatcgtcaa cgcgatcttc atagtcacct ttcatgctgg cgatgatgcc
      961 ctggattgct tcaacgctca tccctggctt gatgtaatcg ctcaggttgt ccagcaacag
     1021 gacgcgcttc tggttgtcac ggatcttttt ctcaacgtcc tgaatttcac gttgcagttt
     1081 gttcttacga cggaacagac gaacaaattc cagtacgtcc tggaatgaag gcttggtagt
     1141 ttccattttt atacccctga taatgtgaga gtcggattcg tttaagcaac cgctattcgt
     1201 tagggccaac attactcata gctgccgcag atgacaatgc ttttatccct tttcactatc
     1261 atacccttta tccttgctga atcgaagcag cagcaagatg attctgaagt tcaggaatta
     1321 tttgcgcaag gcccggcaaa tcagattcga tagcaactca agctgatgtg ccgtttccat
     1381 gttcagccac acataaccat agattggtgt ttccacgact ttcaggtcat ggtggttatc
     1441 gagcaactga cgcagctctt ccacagcatc gttcaatttt tccgtattgg caaaaaccgg
     1501 ctgcggatta ccttcgtaca gcgcatgaac aaggctcagc agtatttgct gcatcatgtg
     1561 ctgggtatca caaagttttt gcgcgttcaa taacacgaaa tg
//
LOCUS       DAJTSA010000266         1597 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000266 DAJTSA010000000
VERSION     DAJTSA010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.268"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..636)
                     /locus_tag="OO369_004299"
     CDS             complement(<1..636)
                     /locus_tag="OO369_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5984298.1"
                     /translation="MILSVFLGQPALTYAGVVIGSTRVVYLSNNADKSISVFSKEEKI
                     PYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEKILRIIHTKGVSLPDDRESVFWL
                     NIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAEN
                     PNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPANSAGGQMTWRFINDYGA"
     gene            complement(750..1322)
                     /locus_tag="OO369_004300"
     CDS             complement(750..1322)
                     /locus_tag="OO369_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HCR5984299.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          474 a          338 c          319 g          466 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aatttcaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
     1321 ataaaaactc cattgatata atatatcctt caccatgaat catcacttat attaataagt
     1381 gtgtttttga ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta
     1441 attacgcaaa aataaaatat agatttattg tttaaatatt atttaaacag taaaaaatca
     1501 agtttgttat cagacattaa tgataattca gaatatattt atatagaaag atagatgaat
     1561 ttattgatgt cagttataaa aaagggccac cggtcgg
//
LOCUS       DAJTSA010000267         1583 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000267 DAJTSA010000000
VERSION     DAJTSA010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.269"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..234
                     /locus_tag="OO369_004301"
                     /pseudo
     CDS             1..234
                     /locus_tag="OO369_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            655..1233
                     /locus_tag="OO369_004302"
     CDS             655..1233
                     /locus_tag="OO369_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984300.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1342..1583)
                     /locus_tag="OO369_004303"
                     /pseudo
     CDS             complement(1342..1583)
                     /locus_tag="OO369_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          524 a          266 c          361 g          432 t
ORIGIN      
        1 actgtcgata tgaaaaagcg agcatcatac tgacgtcaaa caaaggggtc gcagactggg
       61 gagaaatgtt cggagatcac gtgctggcaa cagcgatcct agatcggctg ctacacgctg
      121 aatatcaaag gagagagtta ccggttaaaa gagaaacgta aagctggagt gctgaccaaa
      181 aacacaacgc caatcagtga tgatgaaatg gtggaaaacg gacagcatca gtaacgaaag
      241 tatcttagca ggcatgaaaa tcgtccgcat cccctaaacg gtcaaaatgc ttctgccgtg
      301 aaggagggag aatgccatct catcacttat atctggcgaa tgaaacacag atggtcggcc
      361 ttgcccccaa acacacggcg gtgcaaaatg gaatggcaga aaacttcgtc aaaacgatga
      421 aatatgatta aatcagtgtt atgttcgaac ctgacgtgtt aacagcagta aagtagtctc
      481 gcgaaggtgt tcaaacatta taatgaatgg catcctcata gtgcgcaggg ttatcgcttg
      541 ctgcggaaat atctgaagcg acgtaccagt aatggggaaa atgatagctg gtttctgaaa
      601 atatagagga aaatgtgata tatatatcct gttaatattt aaggagaata taaaatgaaa
      661 gtctcgttta agtctttagg ctatattttt catgatatat ataataaaaa acacactatc
      721 gatgagttca acgatgtggt aagaaaagca gttttatcag gtaaaattaa cgagctgaat
      781 gcatgtcata aagtggcaat ttttcttgcc gaaaaagaca atgagataac aaagaaagac
      841 aaagccaaga taatagatac attaactgaa aactattcca ttgaatttca acaattaatg
      901 aatattagtg agcgcacgtt aaattcatct ttatatatta ctcctggtga aagtggtttt
      961 gtcagttttg ttaatcgtga aggaaaaata tgtcatactg catatgtaaa atcatcagat
     1021 aatagcatga catattacca tgcaaatggt agcagcattg acaaatatat tacggatatg
     1081 tgcggcctta tttgtatgag gcatattgag tctactggca ttatttttta tatgcttgat
     1141 gaaaaagttt tatctgccat tgctgaattt atgaatgaaa aaggatggcg ggctgcgttt
     1201 tgttcagcaa agaacttata taaatgtgtt taattttaga aaaactattt ggtaaaatcc
     1261 aacaagagtc gagaggttcg cgttgcttca gcagggcctc agcctgttca accagactca
     1321 gttgcaggtc gtgacgcaga tttagtggtg gtggttgatt gtcagatacc agccgatgat
     1381 cttgtagtgc atatcatcag actttgagaa gcatatggtt ttgcgggtaa gccgcttaag
     1441 atgtgtccgc aggttcagat tatgtcgctc tatccgctga gtgcatttct tgcttataac
     1501 atgactggtt gaggacaata aagttcggta taccggctca ggcatcggtc atgtagaagg
     1561 caatgctaaa ctggctcagc aaa
//
LOCUS       DAJTSA010000268         1580 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000268 DAJTSA010000000
VERSION     DAJTSA010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.270"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="OO369_004304"
     CDS             complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="OO369_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HCR5984301.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLK"
     gene            complement(1269..>1580)
                     /locus_tag="OO369_004305"
     CDS             complement(1269..>1580)
                     /locus_tag="OO369_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HCR5984302.1"
                     /translation="VAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWG
                     ATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQG
                     A"
BASE COUNT          481 a          400 c          406 g          293 t
ORIGIN      
        1 tttcaaaggg aggatgatga gatcgggaat aaaaacaaat acgactaata atgaatataa
       61 aaccaggacg taagggaaaa cgcctctgac tgcatcatcg aatttgagtt ttgccacccc
      121 tgaaataacg ttaagtacat taccgatagg cggtgtaatt aagccgattg agcagttaat
      181 gataaacatg acaccgaaat aaatcggatc gattcctgcc tctttaacta aaggcattaa
      241 taccggggta agaattaata ccgtcggcgt taaatccatg accatgccga caatcaggat
      301 agccaccata atgacgataa acagcagacg cggtgaatcg accaacggtt gcagtaaatc
      361 agaaaccatc atcggcagtt cagcaatggt aatcagccag gcggaaactt gtgccgaggc
      421 caccagaaac ataaccactg aggtggtttt cgccgcgcca atgagtacat gccaaagtgt
      481 ggcgaaggtc atttcacggt aaataactgt ggcgacaaac agcgcataga aagcagcaac
      541 ggccccggct tcggtagggg taaacagacc ggagcgaaac ccgccaataa tgatgactgg
      601 aagaaacagc gcccagatac cggagacaaa agagtgccag atttcctgca tcgttgcttt
      661 ttgctggcgg ggtaaattaa ggcgactggc ctgccgccac caggtaagca tcagcgttgc
      721 gcccatcatt atgccagggg caatgcccgc cataaacagc ttactgatgg ataatccgct
      781 ggaaacaccg aagataataa acggaatgga agggggaata attggcgcga taatgccgcc
      841 agaggcaatc agccccgccg cccggttgac cgggtagttg gcgctgcgca tcatcggcac
      901 cagtagagcg gcaacggcgg cggtatccgc aacggcagaa ccagaaaggc tcgccataat
      961 catagctgcc agcacgccga cgtagcccag cccgcctggt ttatgcccca ccagtttcat
     1021 cggcaggtca acaatgcgtt ttaacaagcc gcccgcattc atgatttcac ccgccagcac
     1081 aaagaacgga atcgccagga gggagaagct atcggctccg ttcaccagcg tttgtgccat
     1141 gatctggaca tcaaacatgt ccagccagaa cattaacgcc gccccgcaca acaacagtgc
     1201 ccaggcaata ggcaaaccga tagcaatacc acccaacaga cagcccagaa aaatcagcac
     1261 agccatgatt aagctccttg cggtggcgac gttagagaat tgctacgcgt gatgagttga
     1321 tataaatgac gcagttcaaa gaatgcgata acgaggctgg tgggcagaca agcggcatac
     1381 atcaggccga tgggcaaacc gaggatcggt gaataatcgc tccagtcctg aattgttttt
     1441 agcgttgcgc cccaggccag tgcgccacaa ataaataaga ttaaggaatg ggtaaccaga
     1501 gcgactcgtc gttgccatgc gggggagagt ttctccacca gaaaggtgac ctgaacgtgg
     1561 gcgttatcca taaaagccac
//
LOCUS       DAJTSA010000269         1551 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000269 DAJTSA010000000
VERSION     DAJTSA010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.271"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            400..1149
                     /locus_tag="OO369_004306"
     CDS             400..1149
                     /locus_tag="OO369_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR5984303.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
     gene            complement(1319..>1551)
                     /locus_tag="OO369_004307"
     CDS             complement(1319..>1551)
                     /locus_tag="OO369_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5984304.1"
                     /translation="NGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGRLKEY
                     RRIATCYDKTARNYLAIVKLGCIRLFYQRLRN"
BASE COUNT          426 a          329 c          314 g          482 t
ORIGIN      
        1 ccataaacag ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa
       61 ggagtgtccg ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat
      121 atgttctact aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt
      181 ccccttgttg gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct
      241 ttttatgctt gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt
      301 tatttaaatt atgtgatagt acacacaatt ttattatcca gtactttatg ctcttaataa
      361 aataattata aatatgtatc ctctataagg agatgttata tgaaaagaca gctttttact
      421 cttagtattg tcggggtatt ttctttaaat acctttgcat cattccctcc gggaaatgat
      481 gtgacaacaa aacctgacct ttactacctg acaaacgata atgctattga cagtctggca
      541 ttattaccgc ctccaccaca aatcggaagt attgccttcc tgaatgacca ggccatgtat
      601 gaaaaggggc gtttattgcg aaatactgaa agagggaagc tggcagctga ggatgccaat
      661 ctcagtagtg gtggcgtggc aaatgtattt tctgctgctt ttggttctcc gataacggct
      721 aaagattcac cagaattgca taaattactg acaaatatga ttgaggatgc cggagacctg
      781 gcaacgcgtt cagcaaaaga atattatatg cgtattcgac cttttgcttt ttatggtgtt
      841 tctacgtgta atacaaaaga acaggataca ttatccagaa atggctctta tccatcaggt
      901 catacatcga ttggttgggc aacagcactt gtattgtccg aaataaatcc ggcacgccag
      961 gataccattc tgaaacgggg ttatgaactg ggggacagca gggttatttg cggttatcac
     1021 tggcaaagtg atgttgacgc agcacgtatt gttggctctg ctattgtagc aacactgcac
     1081 tcaaatcctg tgttccaggc acaattacaa aaagcgaaag atgaattcgc taacaatcag
     1141 aaaaaataat gcttttagcc agtacgcctt ataaccccga ccttcagggc taatgccagt
     1201 cagttaagca actgactggc tctttttcag ggctgtgggg tatttccagt gcctctcctt
     1261 taccactctc ggaaaggccc tttcccttct tgtcggtaat ttcactaggc tgtgtccctt
     1321 aattacgtaa gcgttgataa aagagtcgga tgcagcccag tttcactatc gccaggtaat
     1381 ttctcgccgt tttgtcgtaa cacgtggcga tgcggcgata ttctttcagc cgcccaaagc
     1441 accgttcaac gacattacga ttgcagtacg catcacgatc aagctgtgca cgtccatccg
     1501 atgccatttt ctcatttgac tttcggggga ttactgcctt tataccgtta t
//
LOCUS       DAJTSA010000270         1511 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000270 DAJTSA010000000
VERSION     DAJTSA010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.272"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..823)
                     /locus_tag="OO369_004308"
     CDS             complement(<1..823)
                     /locus_tag="OO369_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5984305.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1422..>1511)
                     /locus_tag="OO369_004309"
     CDS             complement(1422..>1511)
                     /locus_tag="OO369_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5984306.1"
                     /translation="RTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          398 a          244 c          371 g          498 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggcaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggtta tttttccatt
      721 cagtccaatg ggttagataa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggtgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tggttccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggggggc aatttttcct taaggtatga aaaaaggagc
     1081 tgaagctata taatagctat taatgccact ggtatcagtg aatcatgtat ataaaaaact
     1141 gtgttaagga ggctgtccat tagcggtttt ttattcttct ggaaccgcgg catctttgcg
     1201 tatctttttg tcttctcacc gtctggtcgg tgttttgctg agactgctaa tttcctgtct
     1261 ttgctagtgc tgtactggta ctgcccaatc atgattggcg ggggcgggcg gagttgagac
     1321 cgcagacacg tcgtatgcaa gaacgtgctg cggctggctg gtgaacttcc gatagtgcga
     1381 gtattgaatg atttccagcc gttactgatt ttacgtgcta attagtgaac aaaccactcg
     1441 tcagcagact cccaggtatc tttcagagtt tcctgaacaa aagttttagc tgaatcttta
     1501 tcggcggtgc g
//
LOCUS       DAJTSA010000271         1434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000271 DAJTSA010000000
VERSION     DAJTSA010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.273"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..253
                     /locus_tag="OO369_004310"
                     /pseudo
     CDS             1..253
                     /locus_tag="OO369_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            746..>1434
                     /locus_tag="OO369_004311"
     CDS             746..>1434
                     /locus_tag="OO369_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5984307.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWV"
BASE COUNT          347 a          331 c          445 g          311 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgtcagaaac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt
      661 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
      721 gccaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg
      781 tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg
      841 cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca
      901 gttgcgggtg gaaaaaatcc tgaaccgctc cggcatccag ccgttgcacc ggaagtgttc
      961 gtttgcgaat taccgggcgc agaacgacgg tcagcgacat gcactgagtc aggcgaaatc
     1021 catagctgac gaactgatga caggctgtac gaattttgtg ttcagcggta agcccggcac
     1081 cggaaaaaat catcttgcag ccgcgattgg caaccggctg atggcgaagg ggcgtagcgt
     1141 gattatcgtc accgtgtccg atgtcatgag cgtgttgcat gagagctacg acaacggcaa
     1201 atccggtgaa aaatttttac aggagctttg tggtgttgac ctgctggtcc tggatgaaat
     1261 tggcatgcag cgggatacga aaaacgagca ggtggtactg aaccagattg ttgatcgccg
     1321 gacggcatcg ttacgcggtg tggggatgct gacaaatatt aaccatgcag cgatgaatac
     1381 acttctcggc gagcgggtga tggatcgcat ggtcatgaac ggcgggcgct gggt
//
LOCUS       DAJTSA010000272         1416 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000272 DAJTSA010000000
VERSION     DAJTSA010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.274"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            31..291
                     /locus_tag="OO369_004312"
     CDS             31..291
                     /locus_tag="OO369_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HCR5984308.1"
                     /translation="MAWARHYQQIVREEKETELADDLEKGLPQHLFESLCIDHLQRHG
                     ANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            297..1394
                     /locus_tag="OO369_004313"
     CDS             297..1394
                     /locus_tag="OO369_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5984309.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSIRIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIESLNLGGEHPGIDTSRDNSELFANDGTPTWLKEKRA
                     KEIALDEIIELLNKHHGGTSNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNAL
                     WIELEGVEYAFPDPQTQNETQQAGYDENIPV"
BASE COUNT          440 a          319 c          347 g          310 t
ORIGIN      
        1 gcttatccga tttttgatcg tattgaagaa atggcatggg ctcgtcacta ccagcagatc
       61 gttcgtgaag aaaaagaaac ggaactggcg gacgacctgg aaaaaggtct gcctcagcac
      121 ctgtttgaat cgctctgcat cgaccatttg caacgccacg gtgccaataa acaggcaatc
      181 agccacgcat ttgatgatga tgttgagttt caggagcgca tggcagaaca catccggtac
      241 atggctgaaa ccattgccca ccaccaggtt gatattgatt tggaggacta aaatctatgg
      301 cgctttttca acgagcaata aacacacagg cctatctaaa agcaggaatt atggggtttg
      361 ctggcgacgg caaaacctac acagcaagcg aactggctat tggtctggta atgcttatgc
      421 ggcaacgggg aattgaagct ggtaaccgcc cggtaatgtt tctggacaca gaaacaggct
      481 ctgactgggt taaaccccgc tttgatgcgg aaaatatcga attatttacg gcaaaaaccc
      541 gctcgtttgt tgatctgctt gaagccatta atgaagcgga aagcagcggt tccgtaatga
      601 tcatcgactc catcagtcac ttctggaccg gattatgcga cgagtatgcc agacgccgaa
      661 accgtaaacg aggactggaa ttttcagact gggcgtggct caaacaggag tggcgacgct
      721 ttaccgatcg atttgttaac agtcaggcgc acattattat gtgcgggcgt gcaggatatg
      781 agtatgactt tttcgaaggt gatgatggca aacgccagtt ggcaaaaacc ggtataaaaa
      841 tgaaagcaga aacagaaacc ggttatgaac cctccatccg gatccagatg gagaagcaaa
      901 tgaacataga aaccggtcag gtgtggcgca ccgccagaat actaaaagat cgctccaccc
      961 gtattgatgg acaggtattt tcaaacccaa catttaaaaa ctttcttcct catatcgagt
     1021 ctcttaacct gggaggtgaa caccccggca tcgatacctc acgggataac agtgaattat
     1081 ttgccaacga cggtacacca acatggctaa aagaaaagcg tgcaaaagaa atcgcacttg
     1141 atgaaattat cgagttgctc aacaagcacc atgggggcac cagtaacgac gccaaacgcg
     1201 caaaagctga tttgctggaa aaaacattca cctcccgctc atgggaaaga atcaaaggta
     1261 tggactggcc gacaatcaaa gctggtcgta atgcactatg gattgaactg gaaggcgttg
     1321 aatatgcatt cccggatcca caaacacaaa acgaaacgca acaagcagga tacgatgaga
     1381 atattccggt gtaaaaccaa gtgcattttg ggaatg
//
LOCUS       DAJTSA010000273         1404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000273 DAJTSA010000000
VERSION     DAJTSA010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.275"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..167
                     /locus_tag="OO369_004314"
     CDS             <1..167
                     /locus_tag="OO369_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCR5984310.1"
                     /translation="AIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRM
                     KERRKEWGTL"
     gene            152..790
                     /locus_tag="OO369_004315"
     CDS             152..790
                     /locus_tag="OO369_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCR5984311.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1018..>1404
                     /locus_tag="OO369_004316"
     CDS             1018..>1404
                     /locus_tag="OO369_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984312.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          407 a          257 c          347 g          393 t
ORIGIN      
        1 ccgctattgc cagtacacct cacccgtcct ggcgacgtat ctgtaagaca ctcataaaga
       61 atgatttctg gtgccggact ctctccttca gtccgaacaa accccggcac tatgaacgtt
      121 acctgcagcg tatgaaggaa aggaggaagg aatgggggac tctgtgacac cggagatact
      181 tggcaacatg attcgtcagt atttcagtca ggtacgaact gaaaaagaga gcatacaggc
      241 tctgaatcat ctgcgcagag tgcttcatga ggtcagtcct tttgcacagg agcctgtgga
      301 ctgtgtgttg tgggtgaaag cggatgaggt ggtggccaac gattacaacc ctaatgtcat
      361 ggcactaggt gagaaaaaac tactgaaaca gtctctggag aaagatggtt tcacacaacc
      421 ggtggtggtg tcagaggaga agaatcatta tctggtggtg gatggttttc atcgtcagtt
      481 actggggcgg gaagcagatg caggaaaacg cctgaggggc tggttgcctg tagcctgcat
      541 taatccggag agaaagggac aggccgcacg tattgccgcg accattcgac ataaccgggc
      601 cagggggaaa caccagatca ctttaatgtc tgatattgtc agggatcttt cacgtctggg
      661 ctggacgaat gaacgtattg gtacagagct gggaatggac caggatgaag tgctgaggct
      721 gaaacagatt agcgggctga cagagttgtt tcaggaagag gatttcagct cggcttggac
      781 ggttcgttga tgttgttaaa tatgggcggt atttctgaaa gttgatactt tgggtagaaa
      841 aaatgatacc gaaaactatg gaggatgtgc ttgtcaggtg actggacacc ataggtctta
      901 ggctttgata acgggataag atgagcggct tttctcatac catcaggtgt gatgatggcg
      961 cagatttaca aaatctgatt gattctaaag atgtataagt aaaaatcaag ctttgatatg
     1021 aaaaaacaga tatttattaa taataaacct cctgtagtcc cttactcagg gacgcacgcg
     1081 aaaatattta aatacattga aatccctctg ccgtttttct actttatcta tacatcaggc
     1141 gaaccattcc acatatctgt ccaaaatact gttatttatg tatcaaaata caatggtata
     1201 tttattaata aattagttcc tttttctctt ttatttgaca gggatatatc tgttcttcaa
     1261 agaagagata tttgtgttgt gcgatttacg tcagaggaaa taagtgaaca taacgtttta
     1321 tttgatcatg acattgaacg attaaaaaaa atttccaaag ctcagcttat atctcccgat
     1381 tatgttttga ttgatttttc tagt
//
LOCUS       DAJTSA010000274         1353 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000274 DAJTSA010000000
VERSION     DAJTSA010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.276"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..482)
                     /locus_tag="OO369_004317"
     CDS             complement(<1..482)
                     /locus_tag="OO369_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5984313.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(473..775)
                     /locus_tag="OO369_004318"
     CDS             complement(473..775)
                     /locus_tag="OO369_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5984314.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1047..1319
                     /locus_tag="OO369_004319"
     CDS             1047..1319
                     /locus_tag="OO369_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984315.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          397 a          341 c          288 g          327 t
ORIGIN      
        1 cagactttat aaaacaaggt attaggtgcc acaactgact gaacaaaacc aaaacgctca
       61 taaaatgctc tggcgtcatc atcaattgca tgtaccataa cagccttaat gccaacctga
      121 tcagccagat tgacaacccg gtgaatggca tcacttagta gccatttacc gaaaccatgc
      181 ccctgggtac aaacatcaac agccagacgc cctaacagaa caacagggac aggattgggc
      241 atgttatgcc gcaaactgcg accaagctcg gcatgagtga cggaaccagt agctaatgag
      301 tagtatccca caatacgtaa agtatcctcc aagcaaataa cgaacgtgcg agaagcattg
      361 agcatctggt ttttcatagc ccgaccacgt aaccagttac ttagcacctc atttccacag
      421 tcaaatggct gaagtatatg ctcatccgtc aacaatgctg gcgcggtgac atttatttcc
      481 attcaggctt taccgccatc agacgttcac gcccttctgc attctgcaca ggagcttcca
      541 gttgcgtaat aaacgaatca taacgttcgt tatccagcac aaataaacgt tgatccagga
      601 taatgttttt agcttcctgg tacgccagtt ccatgatgaa atcggtacgg ttcttattga
      661 ccaagctcgc agcataatca ataacggctc gttcctcatc agttgcccgg atgttaatct
      721 gatgttctcg cggtgtttta cggacgcttg cagctgtaga cataaaaacc tccattggta
      781 acaatttgct atgcaattaa tgttatcgca aatggtaaca gaacgctata cagaatcact
      841 tacgggaaaa gcgatcccct gataaaaaca atggcagaga cctgaacatt gtgacgatga
      901 tctgtgtcgg cgcacaaatc atgtctgatt acggccgttt aatgtgattt tgataatgaa
      961 atgcctgaac cacggcagcc tggcggctgc aaatcggggt gtggcagaat atgagctttc
     1021 agagtaacca tccagaatga gttcccatgt cacacaacct ggagcaccag aaagtccaca
     1081 ccagaatggt gaaagaggtt ctgaaagccg tcgccagagc caataatcac ccttataagt
     1141 ccgtatttgc agactttatc accggtcatc cctcctgcac ggtctgtttc tgggaaacct
     1201 tccacaaaat gtacccggat tcaccgtacg aatacgtcac gttctgccat acctgccgtc
     1261 gttttgattt atacgaaaca gaagcggaga tgaaggcgga tgacccgaag tggtggtgat
     1321 tttcagcccc gaccgctgaa acgcctgttc aca
//
LOCUS       DAJTSA010000275         1353 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000275 DAJTSA010000000
VERSION     DAJTSA010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.277"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            114..>1353
                     /locus_tag="OO369_004320"
     CDS             114..>1353
                     /locus_tag="OO369_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5984316.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLV"
BASE COUNT          386 a          329 c          322 g          316 t
ORIGIN      
        1 gacctttctt aaagtactcc aacctactga atcctaaggg atacggcttt aagcctcctt
       61 gcagagttac ttactgtaac ccactgtgta accctctcag ggcatgcaga catatgattc
      121 agctatccag tagtcacaaa cttccagcag tctactatct ctaccagcgt aacggcgtct
      181 actacttccg tcttagggtc agacaatcaa acaatgatag aatgacctca atatcattac
      241 ggaccaagga tagacgcacc gctatggctt actcaaggca cataaaggca gcactcaagg
      301 caatacacgc agacagaccc aacgctacct atgaggaaat gagggaacac cttaaggaca
      361 tcgctgagtg tgaactgtct atgggccgtt cagatctctt tgagcctgac atgagggata
      421 tctaccggga ccaatacgga gaacttggtg agagccttac cgatgctctg gctagtgagc
      481 cgctaagcat tgaccagcat cgttacatca atgaggccct gaaggtgcta aaagcctgta
      541 tgaggcgtat cgaagcggga gacagtcagc ctctgataga ttacgtcgat ctttttaatg
      601 atattgatag gcaagataac caagcagact ctgtgtccct atctgttaat gctccagaag
      661 taaaaccaga agttacgcct agcattacca tagcgagcct ctttgaacaa tacgaagcgg
      721 agaactatca gaactggaag cctgcaacct taagggaaaa caaggcgtca cacgctgcgt
      781 taatcgaaat attcgaccat ctgggtctca atgcagacgc taacagggct gacatgctta
      841 gggtccgtga tgtgctccag cagttaccta ggaaccgtaa gcagcgcttc aaggatgtcc
      901 cacttgctga cctgttgagc cgtgaagata agacagattg tctggatgtc gttaccatca
      961 acaacaagta tcttattaag atggctgctg tctttaggtg ggctgtgcgc aatgacttga
     1021 ttaaaaagaa catgactgag ggtctggagc ttaaagtccc tcagcgtaaa gcctcagggg
     1081 cccgtaacgc cttctctacg gagcaggtgg ggcaactact ggtcgcagct aaggcatact
     1141 ctcagaagac ctctggtaag ccgtatcatt attatgttac cgccttggct gctatcactg
     1201 gggcgagact caatgaaata gcccaactac aggtcaaaga cgtcaggacc actgaggcgg
     1261 gaaccgttta tattcatatc aatgaagacg atagtagtct acccggtaag agcatcaaga
     1321 acgctcatag tgaccgctgt gtgcctctgg tcg
//
LOCUS       DAJTSA010000276         1336 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000276 DAJTSA010000000
VERSION     DAJTSA010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.278"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(207..362)
                     /gene="hokD"
                     /locus_tag="OO369_004321"
     CDS             complement(207..362)
                     /gene="hokD"
                     /locus_tag="OO369_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5984317.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(590..1255)
                     /locus_tag="OO369_004322"
     CDS             complement(590..1255)
                     /locus_tag="OO369_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR5984318.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          331 a          342 c          314 g          349 t
ORIGIN      
        1 ccatccctct tctccctgcg cgctacttaa gtgagtcgat tctatctgcg aaggtgcgcg
       61 aatcaaattc accggaaata aacaacaaaa aacccgccga aacgctgaga gatcacagcg
      121 atgggcgggt taagtgcggg tgcgttgagg atgcctgaca catcagaggt gggcggggat
      181 gggatcagct ccccacccgg tcactcttac ttcctggatt cgtagtctac gaagacagcg
      241 acctccgtct ggccggttcg gattcgtacc tcacagaggt ctttcctcgt taccagtgcc
      301 gtcactatga cggttaaaca gatgacgatc agggcgatta acatcgcctt ttgctgcttc
      361 atagcctgct tctccttgcc tttcggcatg taagaggcta acctacatgt gcaaagcatg
      421 aaattggcct cagattaatg ttaagcgtct tgccggacgc gtaatgttaa ctggggcttt
      481 tctctatctg ccttttggtg ttcatgcctg aagcagatag cctcaagcac ccgcagtcat
      541 tctacttacc tcgccaatat gaaatcaatc agaaaggtgc cccataaaat cactccttct
      601 cttctttacc gtagtggagt tgaccaattt tgataagagg gcgtccctga gatttgcggt
      661 gtagattggt atcgcgcaga gaatacacac agccacaata ttcctgctga tagaattttt
      721 cgcgcttgct gatttcaatc atacgggacg agccgccctg cttgcgccag ttataatccc
      781 agtacaccat gcccggataa tgcgcgacgg ctcgccgccc acagtcgtta acctgctgca
      841 tatttttcca gcgtgaaatg cccagtgaac tgctgatcac actgaaacca ttttcagcgg
      901 cgtacagcgc tgtccgctca aaacgcatgt caaaacacat ggtgcaacgg atccctcgtt
      961 cgggctccca ttccattcct ttggctcgtt caaaccagtt gtctgtgtcg taatcagcat
     1021 caataaacgg cacgccgtgt tgttcagcaa agcgaatatt ctcatcctta cgaattaaat
     1081 actctttctg aggatgaatg ttcgggttgt agaaaaagat ggtgtagtcg atttccgagg
     1141 cctgaagcgc ctccatcacc tcaccggaac atggagcaca gcaagagtgc agtagtagtt
     1201 tgtttgcccc gtttgggagc tccaatttgg gccgtttgaa atcagcaact gtcataaata
     1261 tgtttattga ggtcataaaa atatcaaaga gtgtagcatt agaacggggc tatcggaaac
     1321 agatgtgtga ctgctc
//
LOCUS       DAJTSA010000277         1317 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000277 DAJTSA010000000
VERSION     DAJTSA010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.279"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(75..557)
                     /locus_tag="OO369_004323"
     CDS             complement(75..557)
                     /locus_tag="OO369_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984319.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVNVNGESGVTYENCKLNMKDINDD
                     "
     gene            complement(625..>1317)
                     /locus_tag="OO369_004324"
     CDS             complement(625..>1317)
                     /locus_tag="OO369_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5984320.1"
                     /translation="TPAKGLIALLVGGAYLIIDNWETVGPVVKKVWQEVDQVVRAMGG
                     WEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKLKGLGKISLIGGLL
                     ELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDFVYDMTGWQMPRGD
                     GYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMRVIDLPKTGDPFMK
                     ITHDVGYSPFKR"
BASE COUNT          370 a          335 c          229 g          383 t
ORIGIN      
        1 aaagagaagg taaagaaggt aagtccatac tgtctccact ataaaaaaac cgccaatttc
       61 tggcggctta tatattaatc atcattaata tctttcatat tcaatttgca attttcgtag
      121 gtcactcccg actcgccatt aacattaact ccagctctga tattcccgtt ttcttttaca
      181 tctacaaaaa actggcctct tggcatcttg aacgcaaatg taaaaccaac agtatctgaa
      241 tcaaaatcat catctttata tattgggatc aaaaatgtca tcgtcgctgg tgatgcattg
      301 tttgcaaaat caaatacacc tatacttttg ccgaattcat aagaagcttt tctcaatata
      361 atcgcaccgc tggcagaagg ggatgggcac gatatcctca gttgtctgaa tccatctgta
      421 tccaagtcaa cgtcattatc tgcagatgtt ttaacgttac taataaatcg tgaaacaatc
      481 tcctcatttg ttagtatttt cgcctgagca cagcaagagg ttaatattgc aaccgtcatt
      541 aaaaatggct ttatcacaaa acaaactcca ttgttagcat gttacgcgat aattttgcct
      601 catctttcac cacaaaaaaa gaactcacct tttaaatgga gaatacccaa catcatgggt
      661 gattttcata aagggatcgc ctgttttcgg caaatcgata acgcgcattc caggcggcgc
      721 gttgtcaaac gtcaccttaa gttcgctgcg ggttgccggt gaagatgcaa ggcttaacag
      781 ctgattcctt tccagtgata cattcggggt gtagcgttta tcgggagata aatacccatc
      841 accacgtggc atctgccatc cggtcatgtc atagacaaaa tcatggaact gtttgcccca
      901 ctcgtcgaac ttatcattca gaccaaatcc actgttcagt gcatcagcca caaaattttt
      961 aacaagccag ggatgttctt tttcaaactc ctgcgccatc atgcccagtt caagcaatcc
     1021 accaatcaga ctgatctttc cgaggccctt taatttcagc cctgtttttc ctgccgccgt
     1081 tgtccattga ttctgggcga ctgttgccgc acgaatactt gccaccgcct tcacaccaat
     1141 atacagggca gaaaccgttg ctattgtttt tactgcctgt tcccatcccc ccattgctct
     1201 gacaacctga tcaacctcct gccagacttt tttcacaacc ggaccgaccg tttcccagtt
     1261 atcaattatg agatatgcac caccgacgag aagcgcaatt aatccctttg ccggcgt
//
LOCUS       DAJTSA010000278         1293 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000278 DAJTSA010000000
VERSION     DAJTSA010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.280"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..835)
                     /gene="ipaH4.5"
                     /locus_tag="OO369_004325"
     CDS             complement(<1..835)
                     /gene="ipaH4.5"
                     /locus_tag="OO369_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR5984321.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          360 a          233 c          291 g          409 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagaggtta atctttgcag ggattgcaga acgtgagtcg
       61 acaatggatt ccctgaaatg ttaacattcg tacaaatggg caatgtgatt atattttcgg
      121 gtaatgaagt tagtaggttg ttggaaacat ccagtgccaa tagttcttgc ggtaatctgg
      181 ataatctgcg tagttgattt tcacctacct tcaaatattt taagctctga ggaaattctg
      241 gtagttcctg tagtctgttg ccggaaacaa agagttcttg tagtctatga ggcaattcag
      301 gcaagacagt aaggcgatta ccttcaatac gtaatattgt tagcgttgat ggaagctctg
      361 gtaacgtctc cagactatta aaacgtgccg agagtgattg taaatatggt ggtaatgatg
      421 gcagacatga caaatttcga ttgaatgaaa tattaagctt aatcagttga gatggaagtg
      481 tcgggagtat attcaagttg ttaccattta catgaagttc tgtcaaaagc ggagaatttt
      541 caggtaggga gattaactca ttgttggaaa tattaagttc acgtatatgc aaagggagtg
      601 gtggtaaaga acgtagtttc agtaagctta aattgagaac aggctcccgg gtatccagac
      661 atattttcaa taatcgaacc gcctggatgc gattttctgt tgttgttcct tccctagccc
      721 agtcatccca gatgcggtgg taatctctgg tacactgttc ttctaccgat aaacgagata
      781 tacatgataa gccacaaagg gaacgaaaaa aagaatgatt gttgatcggt ttcatttatt
      841 aacccctcac tattcatatc ttggtctatt aatgcaaata aaacgagaaa gtctttaaaa
      901 aaacaatcct agagctttat cggcgttttc attccggctg gaggaatatg ttcctgtctt
      961 tccagtaacc gtcgattgtt atatcagtct acccatgtta atgtgaccag ttctactttc
     1021 atccagtttg gattgatctt tatcccctat attttcaaac aatttttatc atttaactca
     1081 ttactgtctc gatattccca tagcaaacga tctggttggt tatcccctca aaaaagtcag
     1141 ttgcacgccg taaatccctg ccggtattga gcgaggattt acgggttgtt gttaaggtcc
     1201 gtaatcgggt cacagttttt ccggagtcaa tccggtctgc ggtttatgct tatgcgacgt
     1261 gattatgaat ggtgcagtcg tgagccgttt tca
//
LOCUS       DAJTSA010000279         1275 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000279 DAJTSA010000000
VERSION     DAJTSA010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.281"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            34..435
                     /locus_tag="OO369_004326"
     CDS             34..435
                     /locus_tag="OO369_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5984322.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="OO369_004327"
     CDS             447..818
                     /locus_tag="OO369_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5984323.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..>1275
                     /locus_tag="OO369_004328"
     CDS             805..>1275
                     /locus_tag="OO369_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5984324.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIP"
BASE COUNT          303 a          285 c          396 g          291 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccg
//
LOCUS       DAJTSA010000280         1267 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000280 DAJTSA010000000
VERSION     DAJTSA010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.282"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(277..1185)
                     /locus_tag="OO369_004329"
     CDS             complement(277..1185)
                     /locus_tag="OO369_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984325.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANL
                     ALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQA
                     AAIVELAEATHKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEP
                     MKSIDGIKIIQVDGLNSGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNE
                     IGVSGGSLAALTSPLISTTPVAENVK"
BASE COUNT          250 a          340 c          304 g          373 t
ORIGIN      
        1 agtaacaaca caatcagtat gatgacgagc ttcatcataa ccctttcctt ctgtaaggcc
       61 ccttcctcgg gaggggcttt cccgtttcag ccctttgctg actccccgat tgttgatgag
      121 cgccggacct cgcgcaacgc tttcccagag gcagttttgc cttccccgag cggtcacgca
      181 aaacgttgca acgacctgat catttgcagt ttttagcgga acgcagatat aaaaaatccc
      241 cggctttttg tgccagggac gttcatagct gaaaatttat tttacgtttt cggcgacggg
      301 agttgttgag attaagggtg aagtcaatgc cgccagtgaa ccgcctgaaa cgccaatttc
      361 attgagcaag gagtcaatca gcggagcctg tgtgcggtaa gagagagcgg ctgataatgc
      421 ttgttccgct aggtttccgc cccccacatt acccgtgttt gcatcacccg cagcgctgcc
      481 actattcaga ccatcgacct gaataatctt gataccgtca atagatttca tcggctcaac
      541 ggatttttct attaccgcag gtagcgcctg caacaaggcc agtttgaatt taaggctggt
      601 ttgttcatca gaaagtacgt tgatagcatc gttcagcgca cgttgtgctt ctgcttccgc
      661 cagacccttt ttatgtgtag cttcggctaa ctcaacgata gccgccgcct gcatctctgc
      721 ggcttctttt tctgctttcg cccgcacggt cagttcaacc gctttggttt ctgcatcctg
      781 cgcggcagcg attagggcaa cttgtttagc acgatcggct tctgcggtct ggcgagtggt
      841 ttctacgttt tgttgcgcac ttactgcttc tgcgagtgca aggttagcac gcgcttctgc
      901 ctgggactgt tgttccgatt tggcggcaat agcgatcgat ttcgtctggt tggcgatttc
      961 ggtgacctgc tgctgttcga tctctttaat acgaacttca cgttcagcct caacctttct
     1021 tgagcggacg gcctgctcgc gctcgatctc cgtttcctga atttgtcgtt cagccagaat
     1081 tcgcgtctgc tctgcttcac gacgacgttc agcttcaaaa gccgcaatac gtgcattctg
     1141 ttcggcagta cgggttttaa cctgctgctc ctgctcaagc gtcataaacg cttcttgttg
     1201 ttcggtaatg actccaactt actgatagtg ttttatgttc agataatgcc cgatgacctt
     1261 gtcatgc
//
LOCUS       DAJTSA010000281         1242 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000281 DAJTSA010000000
VERSION     DAJTSA010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.283"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1132
                     /locus_tag="OO369_004330"
     CDS             join(<1..750,750..1132)
                     /locus_tag="OO369_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR5984326.1"
                     /translation="LDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKLSFL
                     KDAIRSRWMWNMRQRLLKAWSEGLAMPESLSHITTESQRRSLVLKAGGKYWHVYMSKK
                     TAGGRNTARYLGRYLKKPPIAASRLAHYNGGGSLSFRYLDHKTGETATETLTQRELVA
                     RLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLS
                     RDPFECVLCGGRMVYRRAIAGLNVEGLKKNARDISLLRYMPA"
BASE COUNT          302 a          299 c          407 g          234 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ctgagcttcc
      481 tgaaagacgc gatacgttcg cggtggatgt ggaatatgcg gcaacggctt ctgaaagcgt
      541 ggtcagaggg gctggcgatg ccggagtcgt tgtcacatat cacgacggaa tcacagagga
      601 gaagcctggt gctgaaagcc ggaggaaaat actggcatgt gtacatgtcg aaaaaaacgg
      661 ccggcgggag gaatacggcg cgctacctgg gtcgttatct gaagaagccg ccgatagcgg
      721 cctcccggct ggcacattac aacggagggg gagcctgagc ttccgttacc tggaccacaa
      781 aacgggagaa acggcgacgg aaacgctgac acagcgtgag ctggtcgcga ggctgaaaca
      841 gcacatcccg gagaagtttt ttaagatggt gaggtacttc gggttccttg ccaaccgggt
      901 gtgtggagag aagctgccgc aggtgtaccg tgctctgggg atggataaac cggaaccagt
      961 ggcgaaagtg tgctatgcac aaatggtgaa acagttcctg agtcgtgacc cgttcgaatg
     1021 cgtgctgtgt ggcggccgga tggtataccg ccgggccatc gcgggactga atgtggaggg
     1081 gctgaagaaa aacgcgcggg atatcagtct gctgaggtat atgccggcct gacacaggct
     1141 aagtgtgccc gggaatcggt gcaagtataa aaaacagcct gttgcggtat aagaatcgac
     1201 catgcagaga gagaaaacac cggagtggag ggaaaagcag aa
//
LOCUS       DAJTSA010000282         1232 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000282 DAJTSA010000000
VERSION     DAJTSA010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.284"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="OO369_004331"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="OO369_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HCR5984327.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          365 a          206 c          294 g          367 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 cgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat
     1021 cgtaaaggaa accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt
     1081 cgatagcgcg agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat
     1141 tattcaatac aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt
     1201 ctctaaaaaa ctcgaaaaaa atggtaactg gt
//
LOCUS       DAJTSA010000283         1160 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000283 DAJTSA010000000
VERSION     DAJTSA010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.285"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            115..>1160
                     /gene="cadB"
                     /locus_tag="OO369_004332"
     CDS             115..>1160
                     /gene="cadB"
                     /locus_tag="OO369_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HCR5984328.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKAS"
BASE COUNT          247 a          255 c          298 g          360 t
ORIGIN      
        1 tttaggatta atcctttttt cgtgagtaat cttatcgcca gtttggtctg gtcaggaaat
       61 agttatacat catgacccgg actccaaatt caaaaatgaa attaggagaa gagcatgagt
      121 tctgccaaga agatcgggct atttgcctgt accggtgttg ttgccggtaa tatgatgggg
      181 agcggtattg cattattacc tgcgaaccta gcaagtatcg gtggtattgc tatctggggt
      241 tggattatct ctattattgg tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca
      301 aaaaacccgc aacaaggtgg cccaattgct tatgccggag aaatttcccc tgcatttggt
      361 tttcagacag gtgttcttta ttaccatgct aactggattg gtaacctggc gattggtatt
      421 accgctgtat cttatctttc caccttcttc ccagtattaa atgatcctgt tccggcgggt
      481 atcgcctgta ttgctatcgt ctgggtattt acctttgtaa atatgctcgg cggtacctgg
      541 gtaagccgtt taaccactat tggtctggtg ctggttctta ttcctgtggt gatgactgct
      601 attgttggct ggcattggtt tgatgcggca acttatgcag ctaactggaa tactgcggat
      661 accactgatg gtcatgcgat cattaaaagt attctgctct gcctgtgggc cttcgtgggt
      721 gttgaatccg cagcagtaag tactggtatg gttaaaaacc cgaaacgtac cgttccgctg
      781 gcaaccatgc tgggtactgg tttagcaggt attgtttata tcgctgcgac tcaggtgctt
      841 tccggtatgt atccgtcttc tgtaatggcg gcttccggtg ctccgtttgc aatcagtgct
      901 tcaactatcc tcggtaactg ggctgcgccg ctggtttctg cattcaccgc ctttgcgtgc
      961 ctgacttctc tgggctcctg gatgatgttg gtaggccagg caggtgtacg tgccgctaac
     1021 gacggtaact tcccgaaagt ttatggtgaa gtcgacagca acggtattcc gaaaaaaggt
     1081 ctgctgctgg ctgcagtgaa aatgactgcc ctgatgatcc tcatcactct gatgaactct
     1141 gccggtggta aagcatctga
//
LOCUS       DAJTSA010000284         1155 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000284 DAJTSA010000000
VERSION     DAJTSA010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.286"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            2..385
                     /locus_tag="OO369_004333"
     CDS             2..385
                     /locus_tag="OO369_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5984329.1"
                     /translation="MLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAFI
                     AVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTACACLTGIFPKMEAFTAEWTFQLAL
                     NVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(386..1030)
                     /locus_tag="OO369_004334"
     CDS             complement(386..1030)
                     /locus_tag="OO369_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5984330.1"
                     /translation="MTPTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSL
                     IDIVQQGFDAGVRLSGIVEKDMISVAIGPPVKLCVAATPEYFARYGKPRHPHDLLNHQ
                     CVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLLYEQIKEYL
                     DTGRLECVLEDWITERPGFQVYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT          295 a          262 c          279 g          319 t
ORIGIN      
        1 ggtgctggtg gcaattctga ttatgctgcc gactctcggc atcggtgata tgaacaatct
       61 ctataaatgg ctgttgaatc ttaactcggt agtgatgccg ctgcgttatt tatgggtatt
      121 tgttgcattt attgcagtcg ttcgcctggc gcagaaatat aaaccagaat atgtctttat
      181 tcgtaataag ccgctggcaa tgaccgtcgg gatttggtgt tttgccttta ccgcctgtgc
      241 ctgtttgacg gggatcttcc cgaaaatgga agccttcact gcagagtgga ccttccagtt
      301 ggcgctgaat gttgcaacgc cgtttgtgct ggtaggatta ggactgatat tcccgctgct
      361 ggcgcgtaaa gcgaatagca aataattatt gtggtcggtg acgctgactc tggcagatct
      421 gacccgtttt tacataatcg agaaatgcgc gcaagccgca ggacatatac tggcggtttg
      481 ggtaatagac ttggaagcct ggacgctctg tgatccagtc ttccagtaca cattctaaac
      541 gaccagtatc aaggtactcc ttgatctgtt catagagcag atacccgata ccagcaccca
      601 tcaaaacggc ctccagttca gcatcaacat catccagaat gatatttcct gcgacagcaa
      661 tttccagctc ctttgcaaat tgccagtgga aaggtttgcc gctgggataa cgaaaaacaa
      721 cacattgatg attaagcaga tcatgaggat ggcggggttt gccatatctt gcaaagtact
      781 ctggcgttgc tgctacacat aatttcactg gtgggccgat cgccacgctg atcatatctt
      841 tttcgacgat gccactaagt cgaacgccag cgtcaaagcc ctgttggacg atatcgataa
      901 gagaatcgtc agttgtgagc tctactttaa tatcaggata ttcacgtgtg aaaccaacca
      961 gtaaaggcat taaaaatata cgtgcagcta cccgggcggc attgattttg agcgttcctg
     1021 tcggtgtcag acgaaaatca ttcatctcat ctaacatgat ctgaatttcg tcgaaagctg
     1081 ggcgcaatcg ttcatagaga ttagaacccg cttccgttaa cgatacgctt cgggttgtcc
     1141 gattgaataa gcgaa
//
LOCUS       DAJTSA010000285         1140 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000285 DAJTSA010000000
VERSION     DAJTSA010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.287"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..730
                     /locus_tag="OO369_004335"
     CDS             <1..730
                     /locus_tag="OO369_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR5984331.1"
                     /translation="TDRIHLKDAARMLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMVN
                     KPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITFT
                     GICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISASGV
                     HNHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFDEVASASLAPISAFDVLISDRP
                     LPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(732..958)
                     /locus_tag="OO369_004336"
                     /pseudo
     CDS             complement(732..958)
                     /locus_tag="OO369_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          298 a          283 c          247 g          312 t
ORIGIN      
        1 aaccgacaga atccatttga aagacgcagc gcgaatgctg gaagtttctg taatgactat
       61 tcgtcgcgat ctccatcagg aagatgaacc tctgccactg accctactgg gtggctatat
      121 tgtaatggtg aataaacccg cgccatccat gccagtaatc catgacgttc caaaaaatca
      181 tcgtgatgac ttacctattg caattctggc tgccggaatg gttaatgaaa atgatctgat
      241 cttctttgat aatggccagg agataccact cgttataagc atgatcccgg atgcaatcac
      301 cttcaccggc atctgttact cacatcgcgt ctttgttgcg ttgaatgaaa agcctaatgt
      361 aacagcaata ctttgtggtg gtacgtatcg tgccagaagt gatgcttttt acgatgccag
      421 taactcttcg ccattagact ctctcaatcc gcgaaaaata tttatttccg ccagcggtgt
      481 gcataatcac tttggcgtca gctggtttaa ccctgaagat cttgccacta agcgtaaagt
      541 gatgaaccgt ggactacgga aaattttgct cgcccgccac gcgttgttcg atgaagtggc
      601 ctctgccagc ctcgcaccga tctctgcatt tgacgttctg attagcgatc gtccgttacc
      661 ggcagattat gttacgcact gccagaatgg ttctgtaaag atcattacac ctgattcaga
      721 agacgaatga cttactgaaa aaacaccaca atcttgttaa acatcgtcgg gttggactga
      781 ttacgttgca ctttcaccac atattccagc ttatctattt ggcttatcac ctactccaga
      841 cgctggtcat ccttgaccag tagccagata tggcttttgt cgcagtcctg aatcggcagg
      901 cagagaatgt cttcaacgtt aagagcgcga cgggcaaaag cccacaaagg tgagtcatta
      961 cacccggatg gtaaacaggc acttccttgt tgtagaagcc gttatattgt ccataatgtc
     1021 tgggttttta atgcatactg ggtaacaacc ggacgaatct tcgaatatca ctggcgctgc
     1081 cagccggttt gaattcatct cacaaccctg catagagcga atctcctgcc tgcacgtcac
//
LOCUS       DAJTSA010000286         1109 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000286 DAJTSA010000000
VERSION     DAJTSA010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.288"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1019
                     /locus_tag="OO369_004337"
                     /pseudo
     CDS             1..1019
                     /locus_tag="OO369_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          312 a          271 c          258 g          268 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggtta
//
LOCUS       DAJTSA010000287         1100 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000287 DAJTSA010000000
VERSION     DAJTSA010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.289"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            55..>1100
                     /locus_tag="OO369_004338"
     CDS             55..>1100
                     /locus_tag="OO369_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR5984332.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          344 a          255 c          178 g          323 t
ORIGIN      
        1 cattaggaga gatacattaa atacaattta ttcacaatct aattccatat ccatatgcga
       61 gaaattaata tgctcagaaa catttcatcc tgtttatttc cacatatcag cacaattaca
      121 tcccccaacc attatttgtc cgaatgggat gattgggaga aacaggggtt accggaagaa
      181 cagcgtactg aggcggtaag aagacttcgt gcatgtctta cctctaaggg gcataaactg
      241 gacctgcgag ccttggcgct ttcctcgcta cctgtactcc ctgcttgcat taaaaagctt
      301 gatgtgagct gtaataaatt aaccatcctt actgatttac ctgaaaatat taaagaactt
      361 attgcaagag ataatttctt aacacatata tctgcattac cacattatct aataactttg
      421 gatgtgtccg aaaatcaatt agagaatctg ccgttattac cagacaccat caaatcacta
      481 agcgcagagt ataataggtt atccacactg ccttcattac ccttgaattt aaaaaaactt
      541 gaggttagga acaacgaact gcaaactctt ccatctctgc cttctaatct taagatactt
      601 aaggttgcgc acaaccatct tactgaactg ccccctttac ctaggagact gcaacttctt
      661 tttgcatata gcaatagatt aagcaactta ccaaacatcc aagaaaatat tatcatgaga
      721 agattttttt attttgaaaa caaccaaata actacaatcc cgacaaatct ttttcgttta
      781 gatcctcata taactattga gattgcaaat aaccccttat cagatcaaac tctgctattc
      841 ttaatacagc aaacttcggt tccaaatttt aacgggcctc agtttcgtat ttccctgtca
      901 gaccaaaaca gactgttttt acgccagatg ttgccgcaaa atttacattc gcgccatatc
      961 agagtcatca ctgaaggggg gcagaacttt cagatccccc ctcttcccga aactgtggca
     1021 gcctggtttc ctgaagcaga tcgtcgggag gtttctacac aatggacttc tttttccacc
     1081 gaggagaatt cccgggcatt
//
LOCUS       DAJTSA010000288         1095 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000288 DAJTSA010000000
VERSION     DAJTSA010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.290"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            39..>1095
                     /locus_tag="OO369_004339"
     CDS             39..>1095
                     /locus_tag="OO369_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HCR5984333.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSENPIASKPESPGRKSSRKPLPAELPRETHRLLPAETSCPACGGVLKEMGETISEQL
                     DIINTAFKVIETIRPKLACSRCDVIVQAPLPPKPIERGYASAGLLARILVSKYMEHIP
                     LYRQSEIYARQGVELSRNTMVRWVSEMADKLRPLYIALNDYVLEAGKVHADDTPVKVL
                     APGNGKTKTGRLWVYVRDDRNAGSSLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADA
                     YAGYNVLYETGRVKEAGC"
BASE COUNT          302 a          275 c          298 g          220 t
ORIGIN      
        1 gcctggcatc cggatataac ccgtgataaa acaagggaat gaacaatgaa ctccccgatg
       61 atattgagct gcttaaagcc atgttgcgta agcaacagag tcggcttcga cagtatgcct
      121 gtcaggtcgc gggctatgag caggaaattg aacggctgaa agcgcaactc gacaggttgc
      181 gtcgtatgtt gttcggccag agttcagaga aaaagcgtca taagcttgaa aatcagatcc
      241 gacaggcaga aaaacgactg tcggaactgg aaaaccggct gaacacagcc agaaatcttc
      301 tggaagatgc atcgtcagtc acagattcac ctgacaccag tcccccgtca gaaaacccga
      361 tcgccagtaa gcctgaatcc ccgggacgaa aatcttcacg aaaaccgctg ccggcagaac
      421 ttccccggga gacacatcgc cttctgcctg ctgaaaccag ttgcccggcc tgtggaggtg
      481 ttctgaaaga aatgggggaa acaatctcag agcaactgga tatcattaat accgccttta
      541 aagttatcga aaccatacgt cccaaactgg cctgtagccg gtgtgatgtc atcgttcagg
      601 caccacttcc tcctaaaccg atcgaacgcg gttatgccag tgcagggtta cttgcacgga
      661 tcctggtcag caaatatatg gaacatatcc ctttatatcg ccagtcagaa atatacgcgc
      721 gacagggcgt ggagctgagc cgtaatacca tggtgcgctg ggtatcagaa atggcagaca
      781 aactccgtcc tctgtatata gcgctgaatg actatgttct ggaggcagga aaggtgcacg
      841 cagatgacac tccggtgaaa gtactggccc cggggaacgg aaagacgaaa acgggtcgtc
      901 tgtgggtata cgtcagggat gatcgtaatg cgggttcatc cctgccggca gccgtctggt
      961 tcgcgtattc ggcagatcgc aaaggagaac atccgcagct ccacctggca aagtatcagg
     1021 gcgtactgca ggctgatgcc tatgcaggtt ataacgtact gtacgaaacg ggccgggtga
     1081 aggaagccgg gtgcc
//
LOCUS       DAJTSA010000289         1069 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000289 DAJTSA010000000
VERSION     DAJTSA010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.291"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(348..1043)
                     /gene="aqpZ"
                     /locus_tag="OO369_004340"
     CDS             complement(348..1043)
                     /gene="aqpZ"
                     /locus_tag="OO369_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCR5984334.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT          300 a          322 c          250 g          197 t
ORIGIN      
        1 gatttttaat cctatctgag tgtttagcat tgttgcgctc ctttcatgtg caatgccata
       61 taaagccact ggcggaggga gcgcgcaggg ggcagccagt cgccgccgcc ccctgcaccc
      121 ccgggctctg gcgaacaaaa tcgccgctgc gcggtacctt cagcttatcc ccttcggcaa
      181 ccgggtcggg cgcgaggtaa cgtcctgtaa gacgcgccct cagcccacat ccatgtgggc
      241 tgccccggcc ttcggggaac gcttcagcga ttttgacgcc accaataccc gtgcggtttg
      301 taatgtcgaa gataaaacaa ccagggcaga taaaatgcca atgctaatta atcacgcttt
      361 tccagcagag tccggtaaat cagaccaccg ataatgccgc cgacaattgg caccacccag
      421 aagaaccaca gttgttctaa tgcccagccg ccctggaaga tagcaaccgc ggtgctgcgc
      481 gccgggttaa cagaagtgtt agtcaccgga atactgatta agtgaatcag ggttaaggcc
      541 agaccaatag cgatcggcgc aaaacctgcc ggagcgaatt tgtcggttgc accgtggatc
      601 actaacagga agcccgcact caataccagt tcaactacca gcgcggaaag catggaataa
      661 ccgcctggtg aatgctcgcc ataaccgtta gaagcaaaac cgctggctgc cgcgtcaaaa
      721 cccgttttac cactggcaat taaatacagc agcgccgctg caacaatacc gccgacaacc
      781 tgggcaatta cgtagccaac gacttctttt gccggaaaac gtccgccagc ccataaacca
      841 atagtgaccg ccgggttaaa atgaccacca gaaatatgac caacagcgaa ggccatcgtc
      901 agaacggtca aaccaaatgc cagcgccacg ccggcaaaac caatgcctaa ttccgggaag
      961 cctgcggcca gtacagcact accacagcca ccaaaaacaa gccagaaagt accaaaacat
     1021 tcagctgcta attttctgaa catatccacc tcaattaaaa attgaccct
//
LOCUS       DAJTSA010000290         1068 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000290 DAJTSA010000000
VERSION     DAJTSA010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.292"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            18..560
                     /locus_tag="OO369_004341"
     CDS             18..560
                     /locus_tag="OO369_004341"
                     /inference="COORDINATES: protein motif:HMM:NF024120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984335.1"
                     /translation="MIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDNYQAIQ
                     NAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKSGLTGS
                     VLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMVNEVET
                     ALFNARALALKIYSMSGWNL"
     gene            787..>1068
                     /locus_tag="OO369_004342"
     CDS             787..>1068
                     /locus_tag="OO369_004342"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HCR5984336.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          314 a          202 c          257 g          295 t
ORIGIN      
        1 acaccaaagg cgatgccatg attggcgtca agcagccttt accaaaagca gttttaagaa
       61 ctcagcatga caaaaataaa gaagcaataa gtatcctgga ttttggtgtt attgatgatg
      121 gtgtgacaga taattaccag gcaatacaaa atgctataga tgccgttgct tcactaccct
      181 ccggcgggga gctgtttatc cctgcgagca accaagcggt ggggtatatt gttggatcca
      241 ctttgcttat tcctggcggt gttaacatca gaggagttgg taaggcatcg caactccgag
      301 caaaaagcgg acttacagga tctgtgttaa ggctgtctta tgattcagac actatcggcc
      361 gttatctgag aaatatacga gtaactggta ataacacctg caatggtatt gacacaaaca
      421 ttacagcaga agactctgtc atcagacagg tttatggctg ggtatttgat aatgtaatgg
      481 tgaatgaagt tgaaaccgca ttatttaatg caagggctct ggcactcaaa atttatagca
      541 tgtcaggctg gaacctgtag agtcggtctt cactttttag gccagtgcgt aagtgttagt
      601 gtcagctcct gccatttcag cagaggaaat tattctgctg atgaaagctt tggcatcagg
      661 attcagcctc aaacgtatgc gtggtcgtca gaggcagtta ggtcagaagc aataatttta
      721 gacagtgaga ccatgtgcat tggttttaaa aatgccgtct atgttcatga ttgccttgat
      781 ttgcatatgg aacaactgga tttagattat tgcggctcaa caggcgtggt tatagagaat
      841 gtaaacggag gattttcttt ctcaaactca tggatagcag cagatgccga tggcactgaa
      901 caatttacgg ggatatattt tagaacgccg gcctcaacgc agtcacataa aattgtcagt
      961 ggtgttcata tcaacactgc aaacaaaaac acggctgcaa acaatcaaag tatagcgata
     1021 gaacagtcgg cgatcttcgt ctttgtaagt ggttgtacgt taactggt
//
LOCUS       DAJTSA010000291         1066 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000291 DAJTSA010000000
VERSION     DAJTSA010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.293"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(570..749)
                     /locus_tag="OO369_004343"
                     /pseudo
     CDS             complement(570..749)
                     /locus_tag="OO369_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            843..>1066
                     /locus_tag="OO369_004344"
     CDS             843..>1066
                     /locus_tag="OO369_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5984337.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAA"
BASE COUNT          285 a          193 c          277 g          311 t
ORIGIN      
        1 ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag gctgaaaaat
       61 tgagtcaata tatcgaaagc ggtagttggc gggcataagt atgtaatgat gatgctgcac
      121 tggtgttgaa tattattcac gccaaacttt cctcattgag caatgtcgtc gcaagtggcc
      181 tcaatgattt tagaccatta tcagaggtcc ggagcggcgg gaagagattt attgctgtgg
      241 aataattgtg gtactgatga ttatttatat cccggattgg tagggaaaag cttttttcat
      301 gcactatttt tgcaaatatt tctatatggg aagtggtttt gttacagatt aaatttataa
      361 agaaatgcat ttggggtgtt gggttgaaga gtagctggta gatgttattg tgccagcagg
      421 ggcaggggaa tgtgccgtat cgatatgctt tggcaattgc gtgtgccgca gatggagatg
      481 aaatgccaat aaaaaagcct gagttcgatg agggacgaac tcaggctggt gttatgtttt
      541 acagtaacac gcctcggcgc gttggctgat tagaactggt agacgatacc aactgccagt
      601 tggtcgtcgg tgttcagtcc atgagcgaga acataatcac tcttatcaag caggttgatc
      661 tgataagcgg tatatatgtt catattctta ttgaagtaat accaggtacc gatttcaata
      721 tagttcagac gatcagaatc tttgtaacct gtagactggc cccctgaatc tccagacaac
      781 caatatcact tatttaagtg atagtcttaa tactagtttt tagactagtc attggagagc
      841 agatgattga tgtcttagga ccggagaaac gcagacggcg tactacacag gaaaagatcg
      901 ctattgttca gcagagtttt gaaccgggaa tgacggtctc ccttgttgcc cggcaacacg
      961 gtgtggcagc cagccagtta tttctctggc gcaagcaata ccaggaggga agtcttactg
     1021 ctgtggccgc aggagaacag gtcgttcctg cctctgaact tgctgc
//
LOCUS       DAJTSA010000292         1018 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000292 DAJTSA010000000
VERSION     DAJTSA010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.294"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(50..190)
                     /locus_tag="OO369_004345"
     CDS             complement(50..190)
                     /locus_tag="OO369_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984338.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            329..1000
                     /gene="queE"
                     /locus_tag="OO369_004346"
     CDS             329..1000
                     /gene="queE"
                     /locus_tag="OO369_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR5984339.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          295 a          205 c          237 g          281 t
ORIGIN      
        1 acatacgaaa gtgaacatta gtatgaaata gcgcattata aatttctcat taaatctgga
       61 taaataaatt gacggcggca tcggtaattt tagattcgtt atcattaaaa actaatacag
      121 aaaaaataaa tataattttg gtgaatgttt tgacatgatt aaatccattt tctttatttt
      181 cttctgacat aatacgttac tccctttaat tattaacgat atgtttcgta tttttcttta
      241 aatctgcatg taacagaatg ttaataagac atgattgttg cggcaggatc tgcaataatt
      301 aacaattatc cacttcacag agaatgctat gcagtacccg attaacgaga tgttccagac
      361 cctgcaaggt gagggttact ttaccggcgt tcccgccatt tttattcgtt tacagggatg
      421 cccggttggc tgtgcctggt gcgacaccaa acacacctgg gaaaagcttg aggatcggga
      481 agtctccctt ttcagcattc tggcgaagac caaagagagt gataagtggg gggcggcgag
      541 cagtgaagac ttgctggctg tcattggtcg tcagggatat accgcgcggc atgtggtgat
      601 tacgggtggt gagccttgca ttcatgattt gctgccactg actgacctgc tcgaaaagaa
      661 cggttttagc tgccagatcg aaaccagtgg aactcatgaa gtacgctgca caccgaatac
      721 ctgggttacc gtatcgccaa agctgaacat gcgcggcggc tatgaagtgt tgtcacaggc
      781 actggagcga gccaacgaaa tcaagcatcc agtggggcgc gtacgcgata ttgaagcact
      841 ggatgaacta ctggcaacgc tgaccgatga taaaccgcga gtcattgcac tgcagccgat
      901 tagccaaaag gatgatgcca cacgattgtg cattgaaacc tgtattgcgc gtaattggcg
      961 tttgtcgatg caaacacata aatatttaaa tattgcctga ttacacattt ataagcgt
//
LOCUS       DAJTSA010000293         1012 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000293 DAJTSA010000000
VERSION     DAJTSA010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.295"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..680)
                     /locus_tag="OO369_004347"
     CDS             complement(<1..680)
                     /locus_tag="OO369_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5984340.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          302 a          171 c          213 g          326 t
ORIGIN      
        1 aacaaagttt gcattattct gggagatagt tgattttctc taagattgac aacgctatca
       61 gaactgaggc caagtatgct ttccggaatt gccgtgatcc ggttctgatt aagccagaat
      121 attcttataa tatgatcatc tctttctgga aaatctggta ttacttccaa agcatttctg
      181 gctgcatcaa gtaattccaa tgacattggt aacctcggaa gtacagacag gtgattgtca
      241 cttacattaa tatattctag tgacgcaggt aattcaggaa gtgcgaacaa atggttatca
      301 ctcacattta tatattccag cgacactggt aattcaggaa gtgcagataa ttgattactg
      361 cttgcattca gctctttcaa tgcccttggt agctcgggga gcattgatag ttggttattg
      421 cttacattaa tttcatcaag attgtcaggc aatcgtggta gagatctgag acctaaacaa
      481 gataagtcca acgatgtttc actgttctcc agacataatt ggagccgggt aaaagcagtt
      541 tccctgtttt ctccggaaat gctgttttta gtccattcaa cccattcgga gagataatta
      601 ttgtgaacat tgtcgattga tgtagttctg taaaaagaga cgtttccagt ggataggggg
      661 ggattattta caggaagcat aataacctcg cagaaggata tcctgataaa atgtgatact
      721 ggggaataat aagctgaaat aaaatgtttc aggattgaaa tttattgttt tcattttaag
      781 gaagtgatgt tgatttaact gtaaaaaaac gatactattt tcttagatgg taaatatctc
      841 gcctgtgcta tattttgttt ttcggttagt taaatatcgt acgttcatat ttgaacgttc
      901 tgccggaatg cattatcaat agaggtaaag tcgcaacccc aaatcgtaaa ggaaaccgta
      961 gcacgtctta agcaagaacg tgctgcggtt ggatgctatt ttttccctga ag
//
LOCUS       DAJTSA010000294         1006 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000294 DAJTSA010000000
VERSION     DAJTSA010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.296"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OO369_004348"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OO369_004348"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR5984341.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          264 a          225 c          233 g          284 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcact ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcgggaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ataagtggcg gaacggacgg gactcg
//
LOCUS       DAJTSA010000295         1004 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000295 DAJTSA010000000
VERSION     DAJTSA010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.297"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..534
                     /locus_tag="OO369_004349"
                     /pseudo
     CDS             1..534
                     /locus_tag="OO369_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          310 a          220 c          210 g          264 t
ORIGIN      
        1 cagttgtcca ggccttccga aatcaaccga ctgaaggcgc tggtcgcgaa actgcagcgt
       61 atgcagttcg gtaaaagctc agaaaaactt cgcgcaaaaa ccgaacggtg gatacaggaa
      121 gcacaggagc gaatcagcgc acttcaggag gaaatggcgg aaacactggg tgagcaatat
      181 gacccggtac tgccatcttc cctgcgccag tcttcagccc gtaaaccgtt accagcctca
      241 cttccccgtg ccccccgggt catccggccg gaagaggaat gctgtccggc ctgtggtggt
      301 gaactcagtc cactgggatg tgatgtgtca gagcagctgg agcttatcag cagcgccttt
      361 aaggttatcg aaaaacaacg ccctaaactg gcctgtcgcc ggtgcgacca tatcgtgcag
      421 gcaccggtgc cttcaaaacc cattgcacgc agttatgccg gagcggggct tctggcccat
      481 gttgttaccg gaaagtatgc agaccatctg ccattatacc gccaaagtga tctactattc
      541 cacacagcta tttagctctc tttttgttga gctcccatga ttctttcatt tattgttact
      601 aattatttta tgttatcatg atgttagaac acgctctgct ctgtgtctct attttatcta
      661 tcatattctg aggattaact acataacaag gcgcaagata tatatacaaa aacaaataat
      721 accgttgcaa taattccttt tagtaaagga ggtgtgatta atttcagggg atttcagagt
      781 aaaaattgta caacatatta aaatatgttc atttcgttat aatacacaca aggaaatatt
      841 aagttagata atatattcta aataaccata gataaaaacg cacataatgg ggaatatttt
      901 atgaaaatac ctgaagcagt aaatcatatt aatgttcaga ataatattga tcttgttgat
      961 ggaaaaacga atccgaataa agcaacaaaa gcattacaaa aaat
//
LOCUS       DAJTSA010000296          974 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000296 DAJTSA010000000
VERSION     DAJTSA010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.298"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            418..>974
                     /locus_tag="OO369_004350"
     CDS             418..>974
                     /locus_tag="OO369_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5984342.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDS"
BASE COUNT          348 a          188 c          157 g          281 t
ORIGIN      
        1 caaagccaac ggtattctga tttctgaagt taatttcaga ctcgtttaag ctccattttc
       61 aaaaaaaata tatcagggtt tgttactaat aacttctgaa aacagaaaaa aaatagagca
      121 gtaattctat ttcagagagg agtaactcca tctatcctcg atacgtactg aacgttatcg
      181 tgatatattc atcatgcttt cttcaccatt attcgagtat agggagagac gcaatgacct
      241 taaatacctt tttatggctg aattaccatt ctcaggaata acagcagact ccttcccata
      301 gacggcttag ggggaaagca atacccggag aaagggtaca aagcaaactg ttttaaaatt
      361 aataagccat attctattta actagaattc ttatcgttga taagaattct agttataatg
      421 attaaatcaa ccaatataca ggcaatcggt tctggcatta tgcatcaaat aaacaatgta
      481 tactcgttaa ctccattatc tttacctatg gaactgactc catcttgtaa tgaattttat
      541 ttaaaaacct ggagcgaatg ggaaaagaac ggtaccccag gcgagcaacg caatatcgcc
      601 ttcaataggc tgaaaatatg tttacaaaat caagaggcag aattaaattt atctgagtta
      661 gatttaaaaa cattaccaga tttaccgcct cagataacaa cactggaaat aagaaaaaac
      721 ctattaacac atctccctga tttaccacca atgcttaagg taatacatgc tcaatttaat
      781 caactggaaa gcttacctgc cttacccgag acgttagaag agcttaatgc gggtgataac
      841 aagataaaag aattaccatt tcttcctgaa aatctaactc atttacgggt tcataataac
      901 cgattgcata ttctgccact attgccaccg gaactaaaat tactggtagt ttctggaaac
      961 agattagaca gcat
//
LOCUS       DAJTSA010000297          933 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000297 DAJTSA010000000
VERSION     DAJTSA010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.299"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(55..243)
                     /locus_tag="OO369_004351"
     CDS             complement(55..243)
                     /locus_tag="OO369_004351"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984343.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
BASE COUNT          245 a          181 c          217 g          290 t
ORIGIN      
        1 ccgcgctgat ggttcttatc cgttttattg aatcggagaa caaactgtga gctatcagcc
       61 tggtggtctg gttttgcctg atttaagccg ccgtgggtat ttgtccaaga atgcttcaag
      121 ctccaatcgt tttggcctgg tgaatggaga tgggttaagc aaagactctc gttgctggtg
      181 tactttttcg cgctggcgct caagcatgga taatgcgatt gcctgtagtt cttcgttagt
      241 cattgtctgc acctggaaag caatttaccg gagtacctca cactacggca aaaatgtttt
      301 attgttgcaa cttgcgtaat tattcaggtc agtttggaac gcactgtata aacataaata
      361 acctgatact gttgtgtgtt ttcaaagaaa acacaggtat ttttcacata aaccttcccc
      421 ttttcatatt cgaccactta atcaaaaaac ggttttatac ttgttgcccc cactggggtt
      481 aaatgtgaga catgtcgcac tttgtgccat gtgtcctcgc tgattttagg cgcggttcat
      541 tcattctttc tgttggcata aagtatggta tcggttgggc gaatgaaagg gactcgggtc
      601 atggtcagac aatacctgag gcgttaattc gttgaaagag attgtgaatg ggggaataac
      661 tgagtcttaa ctgctgtgcg atagcccgga cagatagttg ctcgtcgaat ctgagacgta
      721 atgattctgg caaataagtg cgaaagccgg tgatatctag tgttgtttga gagttcatcg
      781 ttcgcgtcca ttaatacaga tggacgcgat tgttgccaga caggataatt ttcacaagac
      841 atcgcagata gagtacttac gataattttt tattttatac aacagatgcc cacgaaaaga
      901 attattttca atagacacct gcattacaca aag
//
LOCUS       DAJTSA010000298          921 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000298 DAJTSA010000000
VERSION     DAJTSA010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.300"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..197
                     /locus_tag="OO369_004352"
                     /pseudo
     CDS             1..197
                     /locus_tag="OO369_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            297..>921
                     /locus_tag="OO369_004353"
     CDS             297..>921
                     /locus_tag="OO369_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCR5984344.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQD
                     NAQETKELAGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDLKYLHHAT
                     REQRKHITALEMDQENSYVQNLLLAIRGMAEPTTLDNAALLRLTDAIK"
BASE COUNT          292 a          217 c          206 g          206 t
ORIGIN      
        1 ggctacgtaa ttaaaacaga aaataaagaa accccattca ctgtagttga tatcgacggt
       61 ctatcaggaa actttaaaac tcttgttgaa ggtgccacaa aaatgtgcct agtttacatc
      121 ggaaataatc tgcccgcaga aaaaaagccg aatttctggc aaatctgatt gcaatgaaat
      181 taaaaggtga aatctgaaaa aaagaaagcc tgcacagtgt gcaggcctga gtgaagaacc
      241 tgggacattt attcatcact cgcagtaatt ttaatctgag ttgaggttaa aaaacaatga
      301 gcacaaaacc actcttcctg ttacggaaag cgaaaaaatc atccggtgaa cctgacgtcg
      361 tcctgtgggc aagcgacgat tttgaatcga ccagcaccac tctggactat ctgctcgtta
      421 agtcaggtaa aaaactgagc aactatttta aagctgttgc cacaaatttt cctgtcgtta
      481 atgaccttcc ccctgaaggt gagatcgatt ttacctggag tgaacgctat caactcagca
      541 aagactccat gacatgggaa ctaaaaccgg gagcagcgcc agacgacgtt caccaccagg
      601 ataatgctca agaaaccaaa gaactggcgg gaggccagga agaaaacgcg caggcagacg
      661 cccacgagga ttgccaggat tgcgaagtct ctgtagccac tttgcggttc acacagcgtc
      721 ttctgcacat ttttacgtat gctgccgggg atctgaaata cctgcatcat gccacccgtg
      781 aacaacgcaa acacattact gctcttgaga tggatcagga aaacagctat gtccagaatc
      841 tgctgttggc catacgcggc atggcagaac cgacaactct ggataatgcc gccctgctcc
      901 gcctgactga tgcaattaag g
//
LOCUS       DAJTSA010000299          867 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000299 DAJTSA010000000
VERSION     DAJTSA010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.301"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>867
                     /locus_tag="OO369_004354"
     CDS             <1..>867
                     /locus_tag="OO369_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR5984345.1"
                     /translation="LSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFS
                     KDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLM
                     GRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDAM
                     KKGALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGSE
                     NGLQDFTRSLTNLLNALGGSGKSLGESLGSLMTSMSHGVDSLTDISYRVRAFFDEVTL
                     AYRPLNDTQRKIV"
BASE COUNT          262 a          153 c          208 g          244 t
ORIGIN      
        1 tttaagttca gcagcagtta aaacagcagg tgtacttatc agctttcagg ctgtactatc
       61 cgcttatcag cgagtaatgg aagttggcct tcaacgtgca agtgctgaac gttcaattga
      121 tttcgtattt tctaaagatg caaatcaagt aaaagatttc acaaagtcat tagctcaaac
      181 aacgggttta gatatcgctg aactgcaatc acagtttgct ggtttcggtg cttcagctag
      241 agagtctcta ggcattcaag gttctgaaga actttttaga aatatgattg gctattctcg
      301 tctgatgggg cgaagtgaag aagagattaa gcgagcactg acagcattat cacagatggc
      361 tggtaagggt cagattatgg ctgaagagct taaggggcaa ctagctgaag ctgttcctgg
      421 tatggttcag gtattcgcta aagcaaccgg aaagactgaa caggaattat ttgatgctat
      481 gaagaagggg gctttaaaat ctgccgatac tcttcagaag gtcactcagg aactgaataa
      541 acaaatcacc gctaaaggtg gctggaaagc tatttcagaa agtacacagg cacaactagg
      601 gaacttgaaa aactcttgga ataccacgct tgatagtatc ttcagaggtt ctgaaaatgg
      661 tctgcaagat tttacaagaa gcttaaccaa tcttttaaac gctcttggtg gaagtggtaa
      721 gagtcttggt gaatctcttg gtagtctcat gacttctatg tcacatggtg tcgattcatt
      781 gaccgatatt agttacaggg taagggcgtt ctttgatgag gtgacattag cttaccgtcc
      841 actaaatgac actcagagga aaattgt
//
LOCUS       DAJTSA010000300          840 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000300 DAJTSA010000000
VERSION     DAJTSA010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.302"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            46..800
                     /locus_tag="OO369_004355"
                     /pseudo
     CDS             46..800
                     /locus_tag="OO369_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          248 a          177 c          205 g          210 t
ORIGIN      
        1 tactgcaccc attttgttgg acgatgaaat ggaatagccc ctgatatgtc aaagccaaaa
       61 tacccttttg aaaagcgcct tgaagtcgtg aatcactact tcactactga tgatggttac
      121 aggatcatct cggcacgttt tggtgtcccc cgaacccagg tcaggacatg ggttgccctc
      181 tatgaaaaac atggagaaaa aggcttaatt cccaaaccta aaggtgttag tgctgatcca
      241 gagttgcgta ttaaggtcgt gaaagctgtg attgagcagc acatgtccct taatcaggct
      301 gctgctcact ttatgcttgc tggtagtggt tctgtagcca ggtggctgaa ggtttatgaa
      361 gagcgcggag aagctggttt acgcgcgctc aagattggca ccaaaagaaa cattgcaata
      421 tcagttgatc cagaaaaagc ggcatcagca ttggagctgt caaaagaccg acgcattgag
      481 gatcttgaaa ggcaagttcg atttcttgaa acgcggcttg tgtatctaaa aaagctgaaa
      541 gccttagctc atcccacgaa aaagtgaaag tactcaacga gctaaggcag ttttatcctc
      601 ttgatgagct tctcagggct gcggagatac cgcgcagtac gttttattac catctaaagg
      661 ctctcagcaa gcctgacaag tatgcggacg ttaaaaagcg tattggtgag atttatcatg
      721 agaatagagg ccgatacgga taccgtaggg taacgctgtc tcttcatcga gaagggaaac
      781 agattaacca taaagctgtt cacgtatagg aaattgaaaa actaagcgat acccctgtcc
//
LOCUS       DAJTSA010000301          839 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000301 DAJTSA010000000
VERSION     DAJTSA010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.303"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>839
                     /locus_tag="OO369_004356"
     CDS             <1..>839
                     /locus_tag="OO369_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5984346.1"
                     /translation="ITESKIYSAMQKMTNRRHEENWRLRAEACRKKGKPVPEYTPKPA
                     SVATKATNLSFIKALLRAAEREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECP
                     EPLKSVVEFALATGLRRSNIINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACR
                     VLKKQIGNHHRWVFVYKESCTKPDGTKAPTVRRMRYDANTAWKAALRRAGIDDFRFHD
                     LRHTWASWLVQAGVPLSVLQEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVP
                     NSS"
BASE COUNT          269 a          190 c          212 g          168 t
ORIGIN      
        1 acattactga atcaaaaatt tattcagcaa tgcagaaaat gacgaaccgg cgtcatgagg
       61 aaaactggag actcagggca gaagcatgca gaaaaaaagg gaaacctgtt ccagaataca
      121 cgccaaaacc agcgtccgtt gcaacgaagg ctacgaatct ttcatttata aaggccctac
      181 taagagccgc agagcgtgaa tggaaaatgc tggataaggc accaattatt aaagtgcctc
      241 aaccaaagaa taaacggatc cgctggctgg agccccatga agcacaaagg ctgattgatg
      301 aatgtccgga gccattaaag tctgttgttg aatttgcact ggcaacaggc ttaagacgct
      361 cgaacatcat caaccttgaa tggcaacaaa tagatatgca gcgccgggtg gcatggataa
      421 acccggaaga gagtaaatca aaccgcgcaa ttggcgttgc gctgaatgat actgcatgtc
      481 gcgtattgaa aaaacaaatc gggaatcatc accgttgggt atttgtgtac aaggaaagct
      541 gtaccaaacc agacggaacg aaagcgccaa cggtcaggag gatgcggtat gacgcaaaca
      601 cagcctggaa agcggcgctg agacgagcag gtattgatga tttcagattt cacgacttga
      661 gacacacctg ggcaagttgg ctggttcaag ccggagtccc gttgtcagtg ttacaggaaa
      721 tgggaggctg ggagtctatc gaaatggttc gtcgatatgc tcaccttgca cctaatcacc
      781 ttaccgaaca cgcacggcaa atagactcga tcctgaaccc atcggtccca aattcgtcc
//
LOCUS       DAJTSA010000302          836 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000302 DAJTSA010000000
VERSION     DAJTSA010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.304"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(52..720)
                     /locus_tag="OO369_004357"
                     /pseudo
     CDS             complement(52..720)
                     /locus_tag="OO369_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
     gene            complement(710..>836)
                     /locus_tag="OO369_004358"
     CDS             complement(710..>836)
                     /locus_tag="OO369_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HCR5984347.1"
                     /translation="MRVRSQGEMDSLVRNAGFDKCTQRIDVWGIFTVSMAVRRDN"
BASE COUNT          271 a          215 c          186 g          164 t
ORIGIN      
        1 ctgcttaaaa aaagaacaaa tggtagtgtc cgctattgcc agtacacctc aatatggcag
       61 tgctattttg attcgacgtt gatcaagttt ctgataattc caacgacggt cgacgggcac
      121 catccagtca atcaacatac ctaccgccag ccctgtgatg acatcaataa aatgatgctg
      181 ccatgtcgtc agcgtcgaaa tggcgatgag taaaaaccat ccgccacaga ctttacgcca
      241 cctcacagcc agatgctgac gaaagtgacg ccaaagtagc cagcagagaa taatatgcag
      301 cgaaggagac tggttataag gcagatcaaa cagttcaagt tgcgaaaata gccatcctgt
      361 caccccactc acttcaggac ggataaaact aaacttcagc gggtagagaa aaaaaccgca
      421 gcaggccatt accgttgcca gaataagccg gtggacaagt cggcgttgtt cgaatgtcga
      481 gctacaaacg aacagcgaaa atccatataa aagatccaga ctccagtaag gaacaatagt
      541 ccagggaaga aaagggatcg ccgtttccca accgaatacc tgactgggga tatcatggct
      601 gttaaggtcc tgaaccgcgg tgaactgatt aagagatcca taggtgaaga agaaaaacgg
      661 ggccagcaac aataaccagc cagcgccttg tagcaatacg ttccgacgtt cagttatcac
      721 gacgcaccgc catcgaaacc gtaaaaatgc cccacacatc aatccgttga gtgcatttat
      781 caaatccggc attacgcacg agcgaatcca tctccccttg cgaacgcacg cgcatt
//
LOCUS       DAJTSA010000303          832 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000303 DAJTSA010000000
VERSION     DAJTSA010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.305"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..327
                     /locus_tag="OO369_004359"
     CDS             <1..327
                     /locus_tag="OO369_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR5984348.1"
                     /translation="VSETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNG
                     LLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVI
                     LKITYN"
     gene            complement(370..570)
                     /gene="glgS"
                     /locus_tag="OO369_004360"
     CDS             complement(370..570)
                     /gene="glgS"
                     /locus_tag="OO369_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCR5984349.1"
                     /translation="MEHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          252 a          184 c          155 g          241 t
ORIGIN      
        1 gttagtgaga cgtttagtgt agtcatgaca aaagatcctg gtgcggcctg tactgagcaa
       61 ttcaatattt tagggagctt tttcactacg gatattttga gtgattatag ccatctggat
      121 atgggtaatg gactgctatt gaagatattt cataaagatg gaacagcaac ggaatttaac
      181 cgcttctcac aatttgcttc tttttcatcg tctagtgcac cttcggtcac cgcaccattc
      241 agggcagaac ttagtgcgaa cccggcagag actgttgttg agggaccgtt tagtaaagac
      301 gtaatcctca aaatcaccta taactagcat ctaatacaaa cactaaaacg ggccatcagg
      361 cccgttatat cagtgctcta actccagctc tcttgcctgc atcatctgtt ggcaatacca
      421 ggcataacgt gcgcaaaacc aagttctatg ttcttcatcc atgttaccag taacagccag
      481 aatatcgacc gggcgacctt ctgcgtgcat tctggcaaaa ctacgcgcca ggaaatcgaa
      541 attattcaaa gaattaagac tatgctccat aagcacatcc tccggttatc gcataccttt
      601 taaggccata tgctttttct gatttcagta ttccccaatc agtgtagtgc ctttaaacat
      661 aacgtgcgta aatatattgt ccccgatcac actttttagt gaaaaataaa aaaccatatt
      721 aaaatacttt ctcttccaga acttctctaa catgtgttat tattaacaaa gacaaatgta
      781 agcagggaaa ctattccaag gtaaatattg cgtcactaaa tggacattgg ag
//
LOCUS       DAJTSA010000304          829 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000304 DAJTSA010000000
VERSION     DAJTSA010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.306"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..347
                     /locus_tag="OO369_004361"
     CDS             <1..347
                     /locus_tag="OO369_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5984350.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
     gene            702..>829
                     /locus_tag="OO369_004362"
     CDS             702..>829
                     /locus_tag="OO369_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5984351.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIV"
BASE COUNT          245 a          193 c          185 g          206 t
ORIGIN      
        1 cccccgctaa agtgttgccc gaaaactggt ggcaacatcc agctgcatta ggtgcaactg
       61 acagtgatat cgaaatcatc aaacgtcagt ggggtgcgtt ttacggtacg gatctggagt
      121 tgcaactgcg ccgccgaggt atcgatacaa tagtgttatg tgggatctcg accaatatcg
      181 gtgttgaatc caccgcccgc aatgcctggg aacttggctt taatctggtg attgccgaag
      241 acgcctgtag tgccgccagc gccgagcaac acaataacag cattaatcat atctacccgc
      301 gcatcgcccg tgtgcgtagc gtggaagaga tccttcacgc gttatgattt acatcggtct
      361 accgcaatgg tcacatccta aatgggtgcg gttggggatc accagccttg aagagtatgc
      421 ccgccacttt aactgcgtga tacgggattt ttaaaaatca ctaaagaacg cccaagagca
      481 tgtattttct ttagaataat caaagcgttg gattgccatg tcatatgaaa atcggcaaag
      541 ttcatatgta acaaagtgtc ccaatcatgc cccaaaatgc cccacaacaa atatttttgc
      601 cccatgcatg ccccaaaaaa tcactttccc tcactcttga cactgtttat ccatacagtt
      661 aaaaataata ctgtataaaa acacagtgca gagggatttt tatgcgtttt gaaatctgca
      721 tagccaaaga gaaaatgact aaaatgccaa ccggtgctgt ggatgcgtta aaggaagaat
      781 taacccgacg catcagtaaa cgttatgacg atgtagaggt gatcgtaaa
//
LOCUS       DAJTSA010000305          759 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000305 DAJTSA010000000
VERSION     DAJTSA010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.307"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>759
                     /locus_tag="OO369_004363"
     CDS             <1..>759
                     /locus_tag="OO369_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR5984352.1"
                     /translation="ERILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFEL
                     DLEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGT
                     DLFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTID
                     GFSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGD
                     VVNKTQNFEAVLQYQFDFGLRPSIGYSYSKALDVA"
BASE COUNT          211 a          155 c          184 g          209 t
ORIGIN      
        1 gagcgcattc tgaccgaccg tgaaaatggt gaaaaaggcg ataattctca agatacctct
       61 tatgcccgtg taggcgtgaa aggtgaaact cagatcaacc ctgaaatgac tggttacggt
      121 cagtttgaac tagacctgga agcatcaaac agacacaacc ctgaccaaac tcgtttggca
      181 tatgctggtt tgagttacaa agattttggt tcattcgact acagtcgtaa tgtcggtgtc
      241 gcgtatgatg cagaagcatt tactgatatg tttgttgagt ggggcggtga ctcatgggct
      301 ggtactgacc tgttcatgac caaccgtacc aacggtgttg caacctatcg taacacagat
      361 ttctttggca tggttgaagg tctaaatttt gctctgcaat atcagggtaa aaatgaaggt
      421 acaggcaact acaaagcaaa tggcgatggc catggtttat ctgcaactta cactattgac
      481 ggtttctcat ttgctggtgc ttatgcaaac tctgaccgta ctgattggca gtctggtgat
      541 ggtaaaggcg aaagagccga agtttgggcg ttgtcaacaa aatatgacgc taacaatgtt
      601 tatgctgctg tgatgtacgg tgaatctcac aacatgaatt ccgacgacgg tgatgttgtg
      661 aacaaaacac aaaacttcga agctgttctg caatatcagt tcgactttgg tctgcgtcca
      721 tccatcggtt actcctattc taaagcactg gacgtagca
//
LOCUS       DAJTSA010000306          703 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000306 DAJTSA010000000
VERSION     DAJTSA010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.308"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..444)
                     /locus_tag="OO369_004364"
     CDS             complement(<1..444)
                     /locus_tag="OO369_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR5984353.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDD"
BASE COUNT          214 a          171 c          131 g          187 t
ORIGIN      
        1 atcatctgga taatcggcat gagcaataac atgcttgccg gtgcgactgt gtacttcaag
       61 aataaagggc aaatgctgac atttttccag cgccgttttc gcttgtttct gttgattatc
      121 tgccagcgca ataaaccagt cgccgccatt catcaaccac aaagacatct gctggaatgc
      181 cagcgcatcc atcgccatct gttcatgatt gcctcttacc gcacgaaccc aatgttgttc
      241 cagtaactgc agacaacgta aactttgcgg cccacgatcg ataacgtctc ccactgagat
      301 aagtaaatct cgccacggat caaaacgaca atgccataat ttgcggcgca actgctcaag
      361 acaaccgtgt atatcgccag aaagccagat atgtcgccat tgatgacccg caattctctg
      421 ataaacgggc gcaggctgtt tcatcaatat tttcctcccg cgctaaagat cacataatct
      481 taacaagaat gttaaaaaac gctggactca gacagtagag tgtgtgttat ggttgactat
      541 aaagtcagcg aaggaaatgc ttctggcttt taacagataa aaagagaccg aacatgattc
      601 ctgttttcgt caacaaacaa taaaaccctt taaaattaat gcgttgaata aatttacgtt
      661 catcttttaa tccctgacac gtaccattgc atattaatac att
//
LOCUS       DAJTSA010000307          697 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000307 DAJTSA010000000
VERSION     DAJTSA010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.309"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..233
                     /locus_tag="OO369_004365"
     CDS             <1..233
                     /locus_tag="OO369_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5984354.1"
                     /translation="NGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGRLKEY
                     RRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            348..>697
                     /locus_tag="OO369_004366"
     CDS             348..>697
                     /locus_tag="OO369_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5984355.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLA"
BASE COUNT          184 a          180 c          181 g          152 t
ORIGIN      
        1 ataacggtat aaaggcagta atcccccgaa agtcaaatga gaaaatggca tcggatggac
       61 gtgcacagct tgatcgtgat gcgtactgca atcgtaatgt cgttgaacgg tgctttgggc
      121 ggctgaaaga atatcgccgc atcgccacgc gttacgacaa aacggcgaga aattacctgg
      181 cgatggtgaa actgggctgc atccgactct tttatcaacg cttacgtaat taagggacac
      241 agcctaaata gctgcgcgga atagtagatc cctttgaggg aacttagccc ggattgtgcg
      301 atctgatcaa tcgccaaatc aaaacaaatc accaaccgga ctgagcaatg ccgatcatag
      361 caccaatttc ccgtgacgaa cgacgcctga tgcagaaagc catccataaa acacacgata
      421 aaaattatgc ccgcagactg actgccatgc tgatgctgca ccggggcgac cgtgtcagcg
      481 acgttgccag aacgctctgc tgcgcccgtt cctctgttgg acgctggatt aactggttca
      541 cgcagtcggg tgttgaggga ctgaaatcat tacctgccgg gcgtgcccgt cgctggccgt
      601 ttgagcatat ctgcacactg ttacgtgagc tggtaaaaca ttctcccggc gactttggct
      661 accagcgttc acgctggagt acagaactgc tggcaat
//
LOCUS       DAJTSA010000308          694 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000308 DAJTSA010000000
VERSION     DAJTSA010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.310"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            32..493
                     /locus_tag="OO369_004367"
     CDS             32..493
                     /locus_tag="OO369_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCR5984356.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(534..>694)
                     /locus_tag="OO369_004368"
     CDS             complement(534..>694)
                     /locus_tag="OO369_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR5984357.1"
                     /translation="LAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLA
                     ARQHVKKS"
BASE COUNT          204 a          152 c          159 g          179 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc cgccataccc gtcttagctt
      541 ttcttcacat gctggcgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
      601 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      661 acgcggaact gctgttcggt gaggatagcc agaa
//
LOCUS       DAJTSA010000309          691 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000309 DAJTSA010000000
VERSION     DAJTSA010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.311"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(135..265)
                     /locus_tag="OO369_004369"
                     /pseudo
     CDS             complement(135..265)
                     /locus_tag="OO369_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612303.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(263..358)
                     /locus_tag="OO369_004370"
                     /pseudo
     CDS             complement(263..358)
                     /locus_tag="OO369_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          200 a          125 c          166 g          200 t
ORIGIN      
        1 caagggaata tagtttgtgt aagcatatta cttccttatg ttatattttg gtaaaaaatt
       61 tattttttta cattatagtc ggctaaatga agtttactgt aaaaaaacga tactttttcg
      121 ggtggcgctt ccaatcagta gaatatgtgt ttttcaatga aggagccgat gacttttcgt
      181 ggatttccac ggaacgcgag aagcagattg ttttacgtgc cagccgcttg atgcgggtgc
      241 ggagggtcag gttgttacgt tcaagatata gcatgtgctg gatgaagatc ccttttctgg
      301 tcacctgttt atctttcgtg gccgacgggg tgacactgtt aaaatgtaat gaacgcatcc
      361 cacacaatta aacacggagt agtatgatgg ttaaaagggt gaaactggca ggtgtggagc
      421 ctcgcagctt tccggcatca tactgcccaa tacgggatgc ataaagcgtc ctcagactgg
      481 agactccaca aatgaaatat acaccggttg gcgttgatat ggcaaaacat gtcattcaga
      541 ttcacttcat caatgagcac atgtcagcgc cagtgatata agacggtaat ggggatgcgg
      601 acgatttcct ggactctcag aagatgaaac atgtttactg aagaacaaaa aattcgcgct
      661 attgagcttt atttcaaata tggtaagaag c
//
LOCUS       DAJTSA010000310          685 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000310 DAJTSA010000000
VERSION     DAJTSA010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.312"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            52..685
                     /locus_tag="OO369_004371"
                     /pseudo
     CDS             52..685
                     /locus_tag="OO369_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          205 a          172 c          173 g          135 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctcccccat taccccggaa
      241 caaattgaaa tccgtgagct caggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttga
//
LOCUS       DAJTSA010000311          683 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000311 DAJTSA010000000
VERSION     DAJTSA010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.313"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..525
                     /locus_tag="OO369_004372"
                     /pseudo
     CDS             1..525
                     /locus_tag="OO369_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            589..>683
                     /locus_tag="OO369_004373"
     CDS             589..>683
                     /locus_tag="OO369_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5984358.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRL"
BASE COUNT          190 a          139 c          165 g          189 t
ORIGIN      
        1 tgtgctgatc gtgcggatga ttactaccat gaccgtctgg cgctgactgt tgataattca
       61 acgatcgacg acaactacga acactacaca tataacggca cttacaataa tgccgctgat
      121 actcatgttg tagacgttta caacattggt acggctatta ctctggatca ggaagttgat
      181 ctgtccatca ctaataactc tcacgttgcg ggtattacgt tgactcaggg ttatgagtgg
      241 gaagatattg acgacaacac tgtcagcact ggcgtaaaca gcagcgaagt gtttaataac
      301 accatcactg ttaaagattc taccgtaacc tctggttcat ggactgatga aggtactact
      361 ggttggttcg gtaatacagg taatgccagc gattatagtg gtaaatctaa ctttgtcacc
      421 gtagatactg acggagatgg tgttgctgat agcactattg caagctggga tgatgttgca
      481 ttagctgtag ttgcacatcc gaatgttgat aatgctatgc agacctgaat cgccacgggt
      541 ttaacagaca cctcagagtc atttaagatg gcttaaagag aggtgcccat gagcggtaag
      601 cgttatcccg aagagtttaa aactgaagca gtcaaacagg ttgttgatcg cggttattct
      661 gttgccagcg ttgcaacacg tct
//
LOCUS       DAJTSA010000312          660 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000312 DAJTSA010000000
VERSION     DAJTSA010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.314"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            104..>660
                     /locus_tag="OO369_004374"
     CDS             104..>660
                     /locus_tag="OO369_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5984359.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKILPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDS"
BASE COUNT          237 a          120 c          110 g          193 t
ORIGIN      
        1 aagcaatacc tggagaaaga gtacaaagca aactgtatta aacttaataa gttatattgt
       61 atttcactag agttcttatc gttgataaga aattctggtt ataatgatta aatcaacgaa
      121 tatacaggta atcggttctg gtattatgca tcaaataaac aacatacact cgttaactct
      181 attttctttg cctgtgagtt tgagtccatc ttgtaatgaa tattatttaa aagtctggag
      241 tgaatgggaa aagaacggta ccccaggcga gcaacgcaat atcgccttca ataggctaaa
      301 aatatgtttg caaaatcaag gggcggaatt aaatctatct gagttagatt taaaaatatt
      361 acctgattta ccgcctcaga taacaacact ggaaataaga aaaaacctat taacatatct
      421 ccctgatttc ccaccaatgc ttaaggtaat acatgctcaa tttaatcaac tggaaagctt
      481 acctgcctta cccgagacgt tagaagagct taatgtgggt gataacaaga taaaagaatt
      541 accatatctt cctgaaactc taactcattt acggatccat aataaccgat tgcatattct
      601 gccactattg ccaccggagc taaaattact gatagtttct ggaaacagat tagacagcat
//
LOCUS       DAJTSA010000313          656 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000313 DAJTSA010000000
VERSION     DAJTSA010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.315"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(151..648)
                     /locus_tag="OO369_004375"
     CDS             complement(151..648)
                     /locus_tag="OO369_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR5984360.1"
                     /translation="MTTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGNDLKE
                     LRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRDPST
                     PVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKLVE
                     RLMQL"
BASE COUNT          173 a          160 c          144 g          179 t
ORIGIN      
        1 gtttaacgcc ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca
       61 agtgtccatg tctgctattc gcctcgcgat acagctttgc gaagcttacc gtaattgaag
      121 cagtttatgt gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt
      181 atctctgcga acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga
      241 aataacaata tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata
      301 acagagaact cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccctttg
      361 actgaaatag cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat
      421 tttgccgtgg cgtgctgcgc ctcgttctgt ccagaggatg agggagcggg ctttgggtga
      481 aactggattt tgtgagtagt ttaaagcgac ccgcaattct ttaaggtcat taccaacaac
      541 tttgaaaaag tgtttccctt caacgaagcg tactttgttc tcatgatgat tctggcgaat
      601 acgcaccggc tcagtgccgt aaagctgcgc caaaagttcg gtggtaataa caggaa
//
LOCUS       DAJTSA010000314          649 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000314 DAJTSA010000000
VERSION     DAJTSA010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.316"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(24..>649)
                     /locus_tag="OO369_004376"
     rRNA            complement(24..>649)
                     /locus_tag="OO369_004376"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          142 a          193 c          153 g          161 t
ORIGIN      
        1 ctacaaagaa cgcttcttta aggtaaggag gtgatccaac cgcaggttcc cctacggtta
       61 ccttgttacg acttcacccc agtcatgaat cacaaagtgg taagcgccct cccgaaggtt
      121 aagctaccta cttcttttgc aacccactcc catggtgtga cgggcggtgt gtacaaggcc
      181 cgggaacgta ttcaccgtgg cattctgatc cacgattact agcgattccg acttcatgga
      241 gtcgagttgc agactccaat ccggactacg acgcacttta tgaggtccgc ttgctctcgc
      301 gaggtcgctt ctctttgtat gcgccattgt agcacgtgtg tagccctggt cgtaagggcc
      361 atgatgactt gacgtcatcc ccaccttcct ccagtttatc actggcagtc tcctttgagt
      421 tcccggccgg accgctggca acaaaggata agggttgcgc tcgttgcggg acttaaccca
      481 acatttcaca acacgagctg acgacagcca tgcagcacct gtctcacggt tcccgaaggc
      541 acattctcat ctctgaaaac ttccgtggat gtcaagacca ggtaaggttc ttcgcgttgc
      601 atcgaattaa accacatgct ccaccgcttg tgcgggcccc cgtcaattc
//
LOCUS       DAJTSA010000315          636 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000315 DAJTSA010000000
VERSION     DAJTSA010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.317"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            88..153
                     /locus_tag="OO369_004377"
                     /pseudo
     CDS             88..153
                     /locus_tag="OO369_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            381..629
                     /locus_tag="OO369_004378"
     CDS             381..629
                     /locus_tag="OO369_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5984361.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          202 a          131 c          147 g          156 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacac
//
LOCUS       DAJTSA010000316          635 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000316 DAJTSA010000000
VERSION     DAJTSA010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.318"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            18..491
                     /locus_tag="OO369_004379"
     CDS             18..491
                     /locus_tag="OO369_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5984362.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPIYVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          211 a          112 c          127 g          185 t
ORIGIN      
        1 gagatgaacc ctatgcaatg tcctggttaa tatctcagtg tgctcatcag tgcactgata
       61 ataaaaagac ggagacagat gccatttacg ataaagttcg gagttcttat ttgctttcat
      121 gtattctgaa aaaaaacaaa aatgttgggc ttattcttca tgcaccatca tttgtttctg
      181 tgtctgaaaa aatcgccaga attgtgatgg caaattactc ccgtaactgg agtaattccg
      241 agttggccag tgcggtttta atgagtgaat cttctttaaa aagaagaatg tacaaagagg
      301 tcggttctat aagtacattt gtacataaaa taaagcttac agaagctatc aggaaattac
      361 gaaggacaaa tactcccatt tatgtcatat catctgagtt aggttacagt tcaccatcgt
      421 atttttctaa agtcttcttt aaatatttaa agacataccc tcaaaacata aggaagaaaa
      481 atggtcggta atgtcaccaa ttaggaggac ttgcagagcc gcttttttac gatactgaga
      541 tcaaacagac ataatctatg cggagattta cagcagggca tggttcacaa atctgacagc
      601 gatgaattat cggcactgcg ggcagaaaat gctcg
//
LOCUS       DAJTSA010000317          622 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000317 DAJTSA010000000
VERSION     DAJTSA010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.319"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..250)
                     /locus_tag="OO369_004380"
     CDS             complement(<1..250)
                     /locus_tag="OO369_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR5984363.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQ"
     gene            complement(271..>622)
                     /locus_tag="OO369_004381"
     CDS             complement(271..>622)
                     /locus_tag="OO369_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5984364.1"
                     /translation="TVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRH
                     RKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNRR
                     LSESVPCMPHKKAG"
BASE COUNT          141 a          144 c          141 g          196 t
ORIGIN      
        1 tttgcaggta agtcagtaat gtttcttgct gatcgcgcca gacgttctgc acatcaacat
       61 tttcattact gagcagtatt ttaactttgc tgacctgcac gccgttgtcg atccagcgtt
      121 tgatctcgcg gatccggtca atatcggcat cgttgaacag ccgatgaccg ccgtctgtcc
      181 gttgcggttt cagcaatccg taacgcctct gccacgcgcg taacgtgaca gggattaaag
      241 gtaaaccaat tgattttgca agtttttatt ttaccccgct ttcttatggg gcatgcatgg
      301 gacactttca gatagtcttc tgttaagaag ttctatctgt tcgtgattgt tgtctttcat
      361 ccatgctccg taaacattga ataccatttg tgcgtttgtg tggcccatct ggcttgcgat
      421 aaaactagga ttagctcctg cggcaagtga ccagcatgca taggtatgcc tggattgata
      481 cgattttctg tgtctcagac ctgcgcgttt taagatactt gtccatgact ccctgatgga
      541 gtcaacctta tagtgcggtc cggaaaactg ccgctgtttt attacctgag gactaaaaac
      601 aaaagtgcat ttatgcacag ta
//
LOCUS       DAJTSA010000318          620 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000318 DAJTSA010000000
VERSION     DAJTSA010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.320"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>620
                     /locus_tag="OO369_004382"
     CDS             <1..>620
                     /locus_tag="OO369_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HCR5984365.1"
                     /translation="ARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRG
                     LLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRL
                     AADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPV
                     LTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSS"
BASE COUNT          120 a          217 c          163 g          120 t
ORIGIN      
        1 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
       61 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      121 accgtatccg cggccttctg acccagatac atccggcacc ggagcgcgtt ctcggtccga
      181 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      241 cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct cgtctgggta
      301 aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc gttcccggca
      361 cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gctcatcacg ctgcgtaagc
      421 aaagagacga ggtggcgctt gaggtagaac agcgagttct tgctcaccct ctttacccgg
      481 tcctgaccag tatgcccgga gtcggtgtca ggaccgcagc cagactcctc accgaggtcg
      541 cctgccgcgc cttcgcctct gccgcacatc tcgctgctta tgctggcctt gcgccggtaa
      601 ctcggcgatc cggctcgtca
//
LOCUS       DAJTSA010000319          604 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000319 DAJTSA010000000
VERSION     DAJTSA010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.321"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..604
                     /locus_tag="OO369_004383"
                     /pseudo
     CDS             1..604
                     /locus_tag="OO369_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          148 a          172 c          167 g          117 t
ORIGIN      
        1 attcgcctga atttcgcagt gaagccctga agcttgctga acgcatcggt gttactgccg
       61 cagcccgtga actcagcctg tatgaatcac agctctacaa ctggcgcagt aaacagcaaa
      121 atcagcagac gtcttctgaa cgtgaactgg agatgtctac cgagattgca cgtctcaaac
      181 gccagctggc agaacgggat gaagagctgg ctatcctcca aaaggccgcg acatacttcg
      241 cgaagcgcct gaaatgaagt atgtctttat tgaaaaacat caggctgagt tcagcatcaa
      301 agcaatgtgc cgcgtgctcc gggtggcccg cagcggctgg tatacgtggt gtcagcggcg
      361 gacaaggata agcacgcgtc agcagttccg ccaacactgc gacagcgttg tcctcgcggc
      421 ttttacccgg tcaaaacagc gttacggtgc cccacgcctg acggatgaac tgcgtgctca
      481 gggttacccc tttaacgtaa aaaccgtggc ggcaagcctg cgccgtcagg gactgagggc
      541 aaaggcctcc cggaagttca gcccggtcag ctaccgcgca cacggcctgc ctgtgtcaga
      601 aaat
//
LOCUS       DAJTSA010000320          590 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000320 DAJTSA010000000
VERSION     DAJTSA010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.322"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT          217 a          105 c          120 g          148 t
ORIGIN      
        1 atcccgtact ctctggccag ttgggccacg caggccccta gctggtaaga tttttcaata
       61 agatcaagct taaactccat agagtcatag agtaagaggc acgaatcctg cgggggcgaa
      121 gagaatctga cattgataga gtccattaac gttataagag gactctatca tcgccaatca
      181 tcagacattg aaatagacga cactgagatg acgcttacaa ttcttttggt ttctgtaata
      241 agtaatctga catcccccgt gcccagaaga ggagagggat atcaagccca agggttaata
      301 attgatacta cggttaaaat tgccttcagt aaaggaaacg tgattaaatt caggggattt
      361 cagagtgaaa accatacaac atattaaaat ctgttcattt agctataata catacaagaa
      421 agtgtccagt tagataatgt tatctaaata accacagata aaaacgcaca taattggggg
      481 gggggatgaa aatacctgaa gcagtaaatc atattaatgt tcagaataat attgatcttg
      541 ttgatggaaa aacgaatccg aataaagcaa caaaagcatt acaaaaaaat
//
LOCUS       DAJTSA010000321          581 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000321 DAJTSA010000000
VERSION     DAJTSA010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.323"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(141..>581)
                     /locus_tag="OO369_004384"
     CDS             complement(141..>581)
                     /locus_tag="OO369_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR5984366.1"
                     /translation="GGKTGNMFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLR
                     DMINGKQKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELL
                     NDAHPKWSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          147 a          163 c           99 g          172 t
ORIGIN      
        1 caccagcacg tttaacgtcc tgtgccgttt ttcaggtata aaaaaaaccg cataaagcgg
       61 gctctttcaa atgtccatgt ctgctattcg cctcgcggta cagctttgcg aagcgtacaa
      121 gaattgaagc agtttttacg tcaaaaagca ataacttttt tctctatacc aaaagccata
      181 accattggtt tgtacaaaat aaattctgcc acctttagcc aatgctcaat gcgtctttca
      241 caggttctta aactccattt cgggtgtgca tcattcagca gttcagccat tttgcatttg
      301 gtcatccccc gcccctcata ccgttgccga aggacgctaa tcaatcctgg atgctctgcc
      361 agcacctcac ttatgacccg atcaatacat aacgcctctg catcagtaca atgcgccagc
      421 cagctctttt gcttaccgtt aatcatatcc cgcaaaaaag cctcaagttc agacttgttc
      481 agacctgctt ttttcatcct ccggagcgcc tcgttaattg ccgtttttgt cagcttttta
      541 gaggtcaaca tcaggttaaa catattcccc gtcttaccgc c
//
LOCUS       DAJTSA010000322          577 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000322 DAJTSA010000000
VERSION     DAJTSA010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.324"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..98
                     /locus_tag="OO369_004385"
     CDS             <1..98
                     /locus_tag="OO369_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5984367.1"
                     /translation="RLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            156..512
                     /locus_tag="OO369_004386"
     CDS             156..512
                     /locus_tag="OO369_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984368.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
BASE COUNT          183 a          110 c          149 g          135 t
ORIGIN      
        1 ggcgcctgaa tataatctgt cgcgagtgcc ggaagagcga agctatgcag cgtgtactgg
       61 ctttctatca gggtaatttt caggaggcga tactgtgaat gaaattagct atcaggcttc
      121 aattaccgct ggcattcgca tcaaaggaga ggagcatgga aataaaacca gaagatgagt
      181 taagcaatat cgttttattt ccgataaaag aggatgaccc tcgtaatcag gttaattttc
      241 tttataagcc atcggaaaga ccatattgcc atcacgcctc tgtacgggtt gacgaaaaag
      301 agcgtcaggt ccgctgtaaa atctgcggtg cggttgtgga gccgtttgac tggatgctct
      361 ctgtggcaaa aagagaaacc agactggcag atgatgtaag gctattgcgc caggaggaac
      421 aggaaaggcg gagaaatata gaaaagctga tacagattga gcgtaacgcg aaagcgcgga
      481 tacgcagggc gacaaaatcc agaactgaat aattaaattt agcactgtta aaaatttaat
      541 ccttaaccgg agggatttct gcaccctcaa atcatca
//
LOCUS       DAJTSA010000323          575 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000323 DAJTSA010000000
VERSION     DAJTSA010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.325"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            28..>575
                     /locus_tag="OO369_004387"
     CDS             28..>575
                     /locus_tag="OO369_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984369.1"
                     /translation="MTVAQCLDYWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPA
                     SSITVKQWVDLLTEEEKDNPRRARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGS
                     RAEVGDRVLSYHELAKIWLAIERSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLL
                     DNVWTVPKEHSKMGNVIRRPIF"
BASE COUNT          156 a          134 c          151 g          134 t
ORIGIN      
        1 tattttgaag agaaggtgga gaactccatg acggtcgccc agtgtctcga ctactggttc
       61 gacaactacg tctctacaac tctcagagaa aagacccagg cactttatcg atcaacggtt
      121 atgaagcgca tgcatgacgc cttccctaat cgtccggcat cttctatcac ggttaagcaa
      181 tgggttgacc tgcttaccga agaagaaaaa gataatccac gccgagcaag gcaggtgcta
      241 agtcaactaa gatcagcaat tagttggtgc atgcggcgtc agttgataga tagttgcgca
      301 attatgagca tccagccaag ggacttcggc tcccgcgctg aggtagggga tcgggtactg
      361 tcgtatcacg aactggctaa gatttggctt gctattgaaa gaagccgtgc gtctacgtca
      421 aataagctcc ttcatcagat gcttatgctg tggggggcga ggctctcaga gcttaggctg
      481 gcaacaaaga cagaatttga cctgctggac aacgtatgga ccgtaccgaa agagcatagc
      541 aagatgggta atgttatccg ccgtccaatc ttcga
//
LOCUS       DAJTSA010000324          566 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000324 DAJTSA010000000
VERSION     DAJTSA010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.326"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..297
                     /locus_tag="OO369_004388"
     CDS             <1..297
                     /locus_tag="OO369_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCR5984370.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>566)
                     /locus_tag="OO369_004389"
     CDS             complement(405..>566)
                     /locus_tag="OO369_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984371.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          104 g          144 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DAJTSA010000325          559 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000325 DAJTSA010000000
VERSION     DAJTSA010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.327"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>559
                     /locus_tag="OO369_004390"
     CDS             <1..>559
                     /locus_tag="OO369_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR5984372.1"
                     /translation="QQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTTLMGYTQWCC
                     SSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVFTLPCQYWSL
                     VFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHIHLSTTAGGV
                     TSGHTWKNLHFYARKVMSMWRYRITR"
BASE COUNT          127 a          145 c          166 g          121 t
ORIGIN      
        1 tcagcaggga aaccgctggc ttaactggct ggagaaacaa ccggaagggg cagtccgtcc
       61 ggtggtaatt gaatcagtga caaaaatcat ggcctgcggg accacactga tgggctacac
      121 gcagtggtgt tgttcatcac cggactgttg ccatacaaaa aaggtctgct tccggtgtaa
      181 aagccgctcc tgcccgcact gcggggtgaa ggctggcgca cagtggatac agtatctgct
      241 gagcctggtc ccctactgcc cgtggcagca tattgtgttc acacttccct gccagtactg
      301 gtccctggtg ttccacaacc ggtggttact ggcagagatg agccgcattg cagcggatgt
      361 gatactggaa atctgccatc aggcagatgt ggagccgggg atattcacgg tgatccacac
      421 atgggggcgt gaccagcagt ggcatccgca tatccattta tcgacaactg ccggtggtgt
      481 gacgtcgggc cacacctgga aaaatcttca tttttacgcc cgtaaggtga tgagcatgtg
      541 gcgctaccgg ataacacgg
//
LOCUS       DAJTSA010000326          551 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000326 DAJTSA010000000
VERSION     DAJTSA010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.328"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>551)
                     /locus_tag="OO369_004391"
     CDS             complement(<1..>551)
                     /locus_tag="OO369_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HCR5984373.1"
                     /translation="VEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAETRAADKN
                     TDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVSQRDNKT
                     ATFDFSTCSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVREDVMDQ
                     IVQEGSLEYATDVIIHLQQIDIK"
BASE COUNT          134 a          117 c          135 g          165 t
ORIGIN      
        1 atttaatatc aatctgttga aggtgtatta ttacatcagt agcatattcc agactgcctt
       61 cctggacgat ctgatccata acatcctcac gcactttata tcttgtgcat ttcatttcaa
      121 gtgccgtgag tacagccatt actggtgatg gtagctgtgt tgttttcagg ccgtcgatct
      181 gactgatcgc ttgggcgacg gtgttttgcc attctaaaga acaggtgctg aagtcaaatg
      241 tggctgtttt attatcccgt tgcgacacgc tacaggctaa gcgacgtaat tgggtcaatt
      301 tgtctgcttg ctgaacagga actccgggaa agcggcgatg acttaacgtg gtgtgtacaa
      361 cttcgggcgg aaaaacgaca gcaatatctt tcgccagcca aggtaaaaca gcatctgtat
      421 ttttgtcagc cgcacgggtt tctgctacga aagagggttg ttcctgaata atggtggata
      481 agtccgctac cttgagagag tccgttaccg tttgggaagg ttgcaaagaa ttatctttcg
      541 cttgctccac a
//
LOCUS       DAJTSA010000327          533 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000327 DAJTSA010000000
VERSION     DAJTSA010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.329"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT          173 a           78 c          107 g          175 t
ORIGIN      
        1 ttatatgaat atttcagagt tggagttcga atgttgacac ctgagtattt cctgctaaaa
       61 atagccctgc tttacggatg gttgtattgc aataacacga aagtagtaag agattaaagg
      121 tatctgtcag cgataactct gcaagtgatg acattttgta aaccaaataa tgtaaatcag
      181 caatgtatct tttcacttaa ttataacaaa tgtggtattc actaagaatc tgatacgagc
      241 ttacatcacg actattttag tctcatcgcg ggatacaggg atgcaccgat ggatctcagc
      301 acaccgtaat gcaatacctg aacaaataga acagtaaagt ggttcagtat taatgaaagc
      361 tcttattttc cgttgggaaa acttaaaata gatgagaagg ctttttgatt tcttatgtga
      421 tctcatttcg agtattgaga aatttgtgat ttttaaattt ttcaataagc cctgtaaggg
      481 attggggttt tatgagaaat aaagagacgt attgtctttg tttaaacggt acg
//
LOCUS       DAJTSA010000328          523 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000328 DAJTSA010000000
VERSION     DAJTSA010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.330"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            40..390
                     /locus_tag="OO369_004392"
     CDS             40..390
                     /locus_tag="OO369_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5984374.1"
                     /translation="MEPYSLTLDEACHFLKISRPTAINWIRTGRLQATRKDPTKNKSP
                     YLTTRQACIAALQSPLHTVQVSAGDGITEERKCHSSAEVKYGTPVSHCRTVKDLNSLL
                     EQRTKGRRQNSMTS"
BASE COUNT          169 a          123 c          124 g          107 t
ORIGIN      
        1 tatccatttc tcaacaatgt cacagcaaga ggtgaaataa tggagccata cagcctcaca
       61 ctcgatgagg cctgtcattt tctcaagata tccagaccga ctgccattaa ctggatacgc
      121 acagggcgtc ttcaggcaac acgcaaagat cccactaaga ataaatctcc ttacctcaca
      181 acacgacaag cctgcattgc ggctcttcag tctccgctgc atactgtcca ggtgagcgcg
      241 ggtgatggca taacagagga aagaaaatgt cactcttccg cagaggtgaa atatggtacg
      301 ccagtttcac attgccgaac ggtaaaagat ttaaacagtc tcttggaaca aaggacaaaa
      361 ggcaggcgac agaactccat gacaagctaa aggctgaagc atggcgggtc agcaaacttg
      421 gtgaaatacc tgatataacg ttcgaggaag cgtgtgtcag gtggcttgaa gagaaagcac
      481 ataaaaaatc actggacgat gacaaaagcc ggatcggatt ctg
//
LOCUS       DAJTSA010000329          519 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000329 DAJTSA010000000
VERSION     DAJTSA010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.331"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            368..>519
                     /locus_tag="OO369_004393"
     CDS             368..>519
                     /locus_tag="OO369_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5984375.1"
                     /translation="MSQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHRNVLRDIR
                     KMLEEL"
BASE COUNT          129 a          137 c          113 g          140 t
ORIGIN      
        1 tagggtctct ctataggtct tctctatggt ctctctatag gtctctctag ggtctcctct
       61 aggcattacc cccagaagct aactctatgg ccctctatgg tcctctatgg ccctgtacgt
      121 cattctttag gatgccctgt agtttcgtat agcctgatgc ctagagagac cctagagaga
      181 ccctagagtg gctcctagag agaccctccc agtagctccg cacagctaca tcgtcatcat
      241 catagtattc aatctcaaag tcattctgtt tgcatcctgt tgtcctgctg gtctcccttc
      301 gcttggggac tttgagtagg tcagtggggg tgcgacttca gggtgcaaac aaaaaggaaa
      361 tcaagcgatg tctcaacaac cacaacaacc acaacaagaa atcagcatca ttaacctcga
      421 ccagcttgtg tccatgacct cagttgaaat tgctgagctg acaggcaagg agcaccgaaa
      481 tgtccttcgt gatattcgta agatgctcga agaacttaa
//
LOCUS       DAJTSA010000330          484 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000330 DAJTSA010000000
VERSION     DAJTSA010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.332"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>484
                     /locus_tag="OO369_004394"
     CDS             <1..>484
                     /locus_tag="OO369_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5984376.1"
                     /translation="LGALWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFL
                     KIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLAD
                     RFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFT
                     E"
BASE COUNT          109 a          107 c          123 g          145 t
ORIGIN      
        1 actgggagca ttgtgggtgc gcaatggcat ggaggagtcc gcggaatttg aacaacagca
       61 acataatcaa gcggccgcga aaaaacgcat cccggttatc gaagctctgt tacgacatcc
      121 cggtgctttc ctgaagatta ttgcactacg actgtgcgag ttgctgacga tgtacattgt
      181 tactgccttt gcacttaatt attcaaccca gaatatgggg ttaccgcgcg aacttttcct
      241 taatattggt ttgctggtag gtggattaag ctgcctgaca attccctgtt ttgcctggct
      301 tgccgatcgt tttggtcggc gcagggttta tatcacaggc gcgttgatcg gaacgttgag
      361 cgcatttcct ttctttatgg cgcttgaagc acaatctatt ttctggatag ttttcttctc
      421 cataatgctg gcaaacattg cgcatgacat ggtggtgtgt gtgcaacaac cgatgtttac
      481 cgaa
//
LOCUS       DAJTSA010000331          483 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000331 DAJTSA010000000
VERSION     DAJTSA010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.333"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(57..326)
                     /locus_tag="OO369_004395"
     CDS             complement(57..326)
                     /locus_tag="OO369_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5984377.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            383..>483
                     /locus_tag="OO369_004396"
     CDS             383..>483
                     /locus_tag="OO369_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HCR5984378.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNR"
BASE COUNT          125 a          120 c           96 g          142 t
ORIGIN      
        1 accagccaac cgcagcacgt cctgcatacg tcgtgtctgc ggtttttctt tttcgcttac
       61 attgtgtctg gttcttccgg ccactcaata tcaggtgcag ttgatgtatc aacacggttc
      121 agcaacaccc gatacttctt ccaggcttcc agcaacgagt tttcttcctc cgttgcgatc
      181 tccagatcta cagcatcctg aagtggcgca atatgctcac tgaatccctg gatgtagaac
      241 tgtgtggtga cggtcttcca gccattcggc tcctgctgta tcgaagcata ccaggctatt
      301 tcaatatcgc tatgctgcgg cagcatttaa ccccttgtaa ttcatcgcca taattgattt
      361 aattcacaaa taaaactata atatggtgaa attaatgaaa aaaaacacag atgatggggc
      421 taaaatttac acaccactta ccctaaagct ttatgactgg tgggttttgg gagtatcaaa
      481 tcg
//
LOCUS       DAJTSA010000332          473 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000332 DAJTSA010000000
VERSION     DAJTSA010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.334"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..473
                     /locus_tag="OO369_004397"
                     /pseudo
     CDS             1..473
                     /locus_tag="OO369_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          104 a          117 c          147 g          105 t
ORIGIN      
        1 ccgttgaata tggacgggca aaaaagtgga tagcgcacgc gcccttattg cccggggatg
       61 gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt gcgcagttgc acgtcattct
      121 cagacgaacc gatgactgga tggatggccg ccgcagtcgt cacactgatg atacggatgt
      181 gcttctccgt atacaccatg ttatcggaga gctgcccacg tatggttatc gtcgggtatg
      241 ggcgctgctt cgcagacagg cagaacttga tggtatgcct gcgatcaatg ccaaacgtgt
      301 ttaccggctc atgcgccaga atgcgctgtt gcttgagcga aaacctgctg taccgccatc
      361 gaaacgggca catacaggca gagtggccgt gaaagaaagc aatcagcgat ggtgctctga
      421 cgggttcgag ttctgctgtg ataacggaga gagactgcgt gtcacgttcg cgc
//
LOCUS       DAJTSA010000333          468 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000333 DAJTSA010000000
VERSION     DAJTSA010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.335"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>468
                     /locus_tag="OO369_004398"
     CDS             <1..>468
                     /locus_tag="OO369_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984379.1"
                     /translation="SGSTLISRDNKLVENFIDTLENWRDQKQIISVICKDGIKIDDSI
                     ITSFKASKDVGISNGLRIQLTFQEINFKAIVGQTDVSAATGRTSTTNDGGATSKKNTG
                     NTTTSLGSPMLTCKELFSYSASELSDEALKARVTCSKSVSVKNGESTFTASAN"
BASE COUNT          148 a           96 c          103 g          121 t
ORIGIN      
        1 aatccggtag tacccttatc tcacgagata ataagctggt ggagaacttc atcgacactc
       61 ttgaaaactg gcgtgaccag aagcagatta tttcagttat ctgtaaagat ggtattaaga
      121 ttgacgactc cattattaca agctttaaag cctctaaaga tgttggaatt tcaaacggac
      181 tgagaatcca gctaactttt caggaaatta acttcaaagc tattgtaggg caaactgatg
      241 tttcagcagc cactggacgg acttctacca cgaatgatgg cggtgctacc agtaaaaaga
      301 acactggcaa cactacgaca agtcttggaa gccctatgct gacctgtaaa gagcttttta
      361 gctattcggc aagtgagtta tcagatgaag cgttaaaagc ccgtgtaacg tgctctaaga
      421 gcgttagcgt taaaaatggt gagagcacct tcacagcctc agctaatg
//
LOCUS       DAJTSA010000334          459 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000334 DAJTSA010000000
VERSION     DAJTSA010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.336"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>459)
                     /locus_tag="OO369_004399"
     CDS             complement(<1..>459)
                     /locus_tag="OO369_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HCR5984380.1"
                     /translation="LSNRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLLM
                     FNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVRE
                     KLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEHDAGFA"
BASE COUNT          106 a          117 c           90 g          146 t
ORIGIN      
        1 gggcaaatcc agcatcatgt tcgaacacca ctccaacatc tgtttgaaca cctgttaaat
       61 ttctctcttg ttttgtttga acatctgctt cctttctgct tcttctggcc tgaacagatg
      121 cttttcctgc ggctgatttt ttggttaatt tttctctgac agatgccaga tcttcctcaa
      181 tccgaagatg catccattcg tcgccgttat cacaaaaaaa ctcccgcaag gatggttcaa
      241 catcaaccca tcgctcgtta gtcagacggg caatttttgc caacctgttt ttgggtattg
      301 gctttcctgt ttgccagtaa ttgaacatca gcaacaaata cgcgccgtgc tcctctgctg
      361 acaaatgcat ggtgtcagcc aggtaatcag ctatgtacag ttgcatgtat ggtaatgcgg
      421 ccataattgc cccgtatgat gctacccggt tgcttagaa
//
LOCUS       DAJTSA010000335          445 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000335 DAJTSA010000000
VERSION     DAJTSA010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.337"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..373)
                     /locus_tag="OO369_004400"
     CDS             complement(<1..373)
                     /locus_tag="OO369_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984381.1"
                     /translation="MVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFGGM
                     VRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEFDI
                     WNLNETWAFQEKLIFCEDESSL"
BASE COUNT          134 a          106 c           96 g          109 t
ORIGIN      
        1 acagacttga ttcatcctca caaaatataa gtttttcctg gaatgcccat gtctcattga
       61 ggttccagat atcaaattcg aagtcgtgat aacgaaaacg gatcccacca aacttattaa
      121 aacgcagttg tttgacgggc agttcagcca atgtctggaa gagttcctca cgggagcgac
      181 cgatgacgat atcaaggtct gaagagaatc catcgacgcc atacaagcct aagtcacgca
      241 ccatcccgcc aaaaatcctg gccggacagg aacaggccag agtatcaagt aatactctgg
      301 ctttcttatg ctcatcacta agcgtaaaaa aatcggcgat acgctgttca agcagttggg
      361 tgtttgagac cacattcata cagtgatttt agctcatcgt gaaggcgcat taaataacta
      421 tccgtcatgc ctgagattgc gtcgg
//
LOCUS       DAJTSA010000336          445 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000336 DAJTSA010000000
VERSION     DAJTSA010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.338"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            213..>445
                     /locus_tag="OO369_004401"
     CDS             213..>445
                     /locus_tag="OO369_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5984382.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDSRRTVEIHKLDLSDWQAFNKLATKCNAYDGV"
BASE COUNT          141 a          105 c           62 g          137 t
ORIGIN      
        1 tatcgaacct cgcttaccaa cgcgttgtca gtcatctccc ccctctctgg tgcatcgtta
       61 tatccgtgat gagcgatttt tgatattaat atctttcacc atccgctatt ttcaccactt
      121 cagaaaacaa acttattttt attaaaatca attctttttc tttgaacatt caactgcgat
      181 atttgtaaaa acattaaaca ggcaacaata aaatgacatt accaacaact atttattcat
      241 tccccgcata tttaccccgt ttttcgtcca ccgacaaacc agcaaaatta aaatttcatc
      301 aatacgcacg cgctacatta ctgtcaaatc gtggaagaga tcataattgc gatagcagac
      361 gtactgttga gatccacaaa ctagatctct ctgactggca agcattcaat aagttagcaa
      421 cgaagtgtaa tgcttacgat ggagt
//
LOCUS       DAJTSA010000337          443 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000337 DAJTSA010000000
VERSION     DAJTSA010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.339"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>443)
                     /locus_tag="OO369_004402"
     rRNA            complement(<1..>443)
                     /locus_tag="OO369_004402"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           84 a          140 c          104 g          115 t
ORIGIN      
        1 tctgacttaa caaaccgcct gcgtgcgctt tacgcccagt aattccgatt aacgcttgca
       61 ccctccgtat taccgcggct gctggcacgg agttagccgg tgcttcttct gcgggtaacg
      121 tcaatgagca aaggtattaa ctttactccc ttcctccccg ctgaaagtac tttacaaccc
      181 gaaggccttc ttcatacacg cggcatggct gcatcaggct tgcgcccatt gtgcaatatt
      241 ccccactgct gcctcccgta ggagtctgga ccgtgtctca gttccagtgt ggctggtcat
      301 cctctcagac cagctaggga tcgtcgccta ggtgagccgt taccccacct actagctaat
      361 cccatctggg cacatccgat ggcaagaggc ccgaaggtcc ccctctttgg tcttgcgacg
      421 ttatgcggta ttagctaccg ttt
//
LOCUS       DAJTSA010000338          438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000338 DAJTSA010000000
VERSION     DAJTSA010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.340"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..203)
                     /locus_tag="OO369_004403"
     CDS             complement(<1..203)
                     /locus_tag="OO369_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR5984383.1"
                     /translation="MQSERIYLVWAHPRHDSLTANIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDKRYSP"
     gene            303..>438
                     /locus_tag="OO369_004404"
     CDS             303..>438
                     /locus_tag="OO369_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5984384.1"
                     /translation="MRMNMSDFATSFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     L"
BASE COUNT          114 a          104 c           93 g          127 t
ORIGIN      
        1 ggagaataac gtttatccat attcttccag tctggttcat cttccgacgt catcaccggg
       61 ttgaaattac gccgatataa atcgagttcc gtcacctgta ttttccgctc cattgcccgc
      121 tgatggatcg catcagcaat atttgcggtc aatgaatcat gacgaggatg ggcccatacc
      181 aaataaatac gttcagactg cataatttta cctccacgtt aagttctgga gcgcacttta
      241 cgcaggcggc ataatcctgt aaacgtgatg attcaggatg agttaatgaa ggattttcac
      301 taatgcggat gaatatgtct gactttgcca cttcctttgc cgtggcccgt aatcaaagct
      361 ttcgtgcagc aggcgatgag ttaggcttat cctcgtccgc cattagccat agtattaaaa
      421 cactggaaca acgtctta
//
LOCUS       DAJTSA010000339          434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000339 DAJTSA010000000
VERSION     DAJTSA010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.341"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            35..434
                     /locus_tag="OO369_004405"
                     /pseudo
     CDS             35..434
                     /locus_tag="OO369_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAJTSA010000340          428 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000340 DAJTSA010000000
VERSION     DAJTSA010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.342"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>428
                     /gene="istA"
                     /locus_tag="OO369_004406"
     CDS             <1..>428
                     /gene="istA"
                     /locus_tag="OO369_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HCR5984385.1"
                     /translation="SRNTVKRYLQAKSEPPKYTPRPAVASLLDEYRDYIRQRIADAHP
                     YKIPATVIAREIRDQGYRGGMTILRAFIRSLSVPQEQEPAVRFETEPGRQMQVDWGTM
                     RNGRSPLHVFVAVPGYSRMLYIEFTDNMRYDTLETCHRNA"
BASE COUNT          100 a          118 c          105 g          105 t
ORIGIN      
        1 tctcccgcaa taccgttaaa cgttatttgc aggcaaaatc tgagccgcca aaatatacgc
       61 cgcgacctgc tgttgcttca ctcctggatg aataccggga ttatattcgt caacgcatcg
      121 ccgatgctca tccttacaaa atcccggcaa cggtaatcgc tcgcgagatc agagaccagg
      181 gatatcgtgg cggaatgacc attctcaggg cattcattcg ttctctctcg gttcctcagg
      241 agcaggagcc tgccgttcgg ttcgaaactg aacccggacg acagatgcag gttgactggg
      301 gcactatgcg taatggtcgc tcaccgcttc acgtgttcgt tgctgttccc ggatacagcc
      361 gaatgttgta catcgaattc actgacaata tgcgttatga cacgctggag acctgccatc
      421 gtaatgcg
//
LOCUS       DAJTSA010000341          424 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000341 DAJTSA010000000
VERSION     DAJTSA010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.343"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..279)
                     /locus_tag="OO369_004407"
     CDS             complement(1..279)
                     /locus_tag="OO369_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984386.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
BASE COUNT           94 a           99 c          133 g           98 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagg
//
LOCUS       DAJTSA010000342          420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000342 DAJTSA010000000
VERSION     DAJTSA010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.344"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..420
                     /locus_tag="OO369_004408"
                     /pseudo
     CDS             1..420
                     /locus_tag="OO369_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          126 a           92 c          130 g           72 t
ORIGIN      
        1 atgaacatcc gcatcttcct gttgaaagtc gtcgtcaggc aaagggaact cgcttcctgt
       61 ggcagcacag cgataaatgg atgaccgaat ccaggcagga aaagctgata tggctgcgtg
      121 cacagatgaa gctgacgagc ctgtgctggg cgctgaaaga actggcaaag gatatctgga
      181 gcagaccatg gagcgaggaa agacgcaacg actggcagag atggctgcgg ccaacagtga
      241 cgtccccatg atgaagaatg tggctaaaac gatagggaaa aggttgtacg gtatcctgaa
      301 cgccatgcgc catggcgtat caaacggaaa tgcggaagca ctgaacagca agatcaggct
      361 gctgagaata aaagccaagg gctaccgcaa ccgggagcgc tttaagctgg gagtgatgtt
//
LOCUS       DAJTSA010000343          418 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000343 DAJTSA010000000
VERSION     DAJTSA010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.345"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..127
                     /locus_tag="OO369_004409"
     CDS             <1..127
                     /locus_tag="OO369_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="holin"
                     /protein_id="HCR5984387.1"
                     /translation="VSPSQWAAIGVLGSLLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            127..>418
                     /locus_tag="OO369_004410"
     CDS             127..>418
                     /locus_tag="OO369_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCR5984388.1"
                     /translation="MSPSLRKAVAVAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLVTVARQINPYIKVDIPET"
BASE COUNT          119 a           86 c          109 g          104 t
ORIGIN      
        1 ggtcagtcca tcacagtggg ctgcgattgg tgtgctggga agtctgcttc tggggcttct
       61 gacttatctg acgaatctgt atttcaaaat aagagaagat aagcgtaagg ctgcgagagg
      121 tgaataatgt cgccatcatt acgcaaggct gttgcagttg ctattggtgg cggggctgtt
      181 gctatagcat ctgtgttaat cactggccca ggtggtaacg atggtctgga aggtgtcagc
      241 tacataccat acaaagatat cattggtgta tggactgtat gtcacggaca caccggaaaa
      301 gacatcatgc ctggtaaaac gtataccgaa gcagaatgca aagctctcct gaataaagac
      361 cttgtcactg tcgccagaca aattaacccg tacatcaaag tcgatatacc ggaaacaa
//
LOCUS       DAJTSA010000344          415 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000344 DAJTSA010000000
VERSION     DAJTSA010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.346"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..259)
                     /locus_tag="OO369_004411"
     CDS             complement(<1..259)
                     /locus_tag="OO369_004411"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984389.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARC"
BASE COUNT          114 a           94 c           93 g          114 t
ORIGIN      
        1 tgcagcgggc aatttccgga tacccttcgt catcaacacg tacaaaccag aagaccagtt
       61 ttttgtttcc ggcatccaca aagaacggaa tattcaggtc tgcgcaacac tcaacggcat
      121 cgtcaaaatt atcaaagcgc agaacctcag cttcttcttc gtcaaaaaaa tcatcttcgt
      181 gaagttttac gacatagcgg aggagtttgc ttctttgagg tgcaggtttt cgtttgcgtg
      241 gtttagctga acgggccata taactacctg aaagacaatg atattgtctg tttttataac
      301 ggtaatcaca ggccatgaca agccgcagcc gtcaggccgc ctactcgggg cggtttagaa
      361 cgcttattta gtaccagatc ttacttataa taaaggggct tggcagtttg gataa
//
LOCUS       DAJTSA010000345          388 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000345 DAJTSA010000000
VERSION     DAJTSA010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.347"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>388
                     /locus_tag="OO369_004412"
     CDS             <1..>388
                     /locus_tag="OO369_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR5984390.1"
                     /translation="HQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQ
                     LWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSENELAEQLIE"
BASE COUNT           84 a          128 c          107 g           69 t
ORIGIN      
        1 tcaccagatt gtgaatcgcc tggacatcat gctgccgggc aatcgcccct tcgttgcccc
       61 cagtgccgtt attcaggccc gccagcgcct gggaagtgag gctgtccgcc gcgtgttcac
      121 gaaaacagcg cagctctggc ataacgccac gccgcatccg cactggtgcg gcctgaccct
      181 gctggccatc gatggtgtgt tctggcgcac accggataca ccagagaacg atgcagcctt
      241 cccccgccag acacatgccg ggaacccggc gctctacccg caggtcaaaa tggtctgcca
      301 gatggaactg accagccatc tgctgacggc tgcagccttc ggcacgatga agaacagcga
      361 aaatgagctt gctgagcaac ttatagaa
//
LOCUS       DAJTSA010000346          388 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000346 DAJTSA010000000
VERSION     DAJTSA010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.348"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(40..>388)
                     /locus_tag="OO369_004413"
     CDS             complement(40..>388)
                     /locus_tag="OO369_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5984391.1"
                     /translation="QIKPFLEKAMTMYNDVLFPGEDINKPISIAAANRFVNRIRGGMD
                     LGYWRTHDFRRTLVTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELH
                     ADKLFWHIRSISD"
BASE COUNT           92 a           89 c           87 g          120 t
ORIGIN      
        1 ttcgtgaagc tgttgaaggg tggattctta acggtggcgt taatcagaaa tgctcctgat
       61 gtgccagaac aatttatcag cgtgaagctc atacgctttg cgctgagcct ctatccagtc
      121 gtgtttattg tatacggaca ttatcccgcc aagttcatga ccgagcattc gctcagtaac
      181 atgaggctcg acattcatct cggacagccg tgtaacaagc gttcttctga aatcatgtgt
      241 tcgccagtaa cctagatcca tccctcccct tattctattt acgaatcggt tggctgcagc
      301 gatgctgatc ggtttgttta tgtcttctcc agggaaaaga acatcattgt acattgtcat
      361 ggccttttcg agaaaaggct taatttgt
//
LOCUS       DAJTSA010000347          384 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000347 DAJTSA010000000
VERSION     DAJTSA010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.349"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..252)
                     /locus_tag="OO369_004414"
                     /pseudo
     CDS             complement(1..252)
                     /locus_tag="OO369_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           88 a          123 c           86 g           87 t
ORIGIN      
        1 ctcactgtct ccgttatggc gtacaccaca tcttccagta atcgccgctt caccggactc
       61 aggaccccgt atcaccatcc ggaacccctt atccgccaca tgccaggaga acctttccat
      121 aaactcgtcg ccactcatca caaggttttc ttctcgtttt gtccggtggc tgttgtaacg
      181 caggccaatt tcatcctgac cggcataatg ttccaggcgg ctcatctgca ccggctgctt
      241 ctccagccag ttaagccatc ggtttccctg ctgaaaaata tcggcaaaac ggggaagcat
      301 cagaaaggcg gaataacgcc atccggccag tgaaccgtac cgcattcagg gcaatacatg
      361 ccgtcggcgt tgataccggc agga
//
LOCUS       DAJTSA010000348          375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000348 DAJTSA010000000
VERSION     DAJTSA010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.350"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            260..318
                     /locus_tag="OO369_004415"
                     /pseudo
     CDS             260..318
                     /locus_tag="OO369_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
BASE COUNT           99 a           83 c          105 g           88 t
ORIGIN      
        1 aaaaaaaatc agcgtcaaga aggtttcatt aacacatatc cctggcccaa aaagccgggg
       61 attttttata tctgcgttcc gctaaaaggt gcaaatgctc aggacgttgc agcgttttgc
      121 gtgaccgctc ggggaaggca aaactgcttc agggaaagcg ttgcgcgggg tccggcgctc
      181 atcaacaatc ggggagtcag caaggggctg aaacgggaaa gcccctcccg aggaaggggc
      241 cttatagaag gaaagggtta tgatgaagtt cgtcatcata ctggttggtt gttactcgta
      301 agtttcgcag cttattaaca gccaatcaga ggggggagaa atcctccctc acccttgtgc
      361 ctttacttta ggttg
//
LOCUS       DAJTSA010000349          375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000349 DAJTSA010000000
VERSION     DAJTSA010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.351"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>375
                     /locus_tag="OO369_004416"
     CDS             <1..>375
                     /locus_tag="OO369_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5984392.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRLMRQNALLLERKLAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDRKALHWAVTT"
BASE COUNT           86 a           96 c          110 g           83 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggctca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aactcgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgta aggcactgca
      361 ctgggcggtc actac
//
LOCUS       DAJTSA010000350          373 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000350 DAJTSA010000000
VERSION     DAJTSA010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.352"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..373
                     /locus_tag="OO369_004417"
                     /pseudo
     CDS             1..373
                     /locus_tag="OO369_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
BASE COUNT          106 a           83 c           88 g           96 t
ORIGIN      
        1 cagtggcaat ttctgctgta gctgcatcag tactgatggc gatgtctgct caggcagcag
       61 agatttacaa taaagacagt aacaagctgg atctgtacgg aaaagttaat gctaagcact
      121 acttctcttc taacgatgca gatgatggcg acacaactta tgttcgtctg ggcttcaaag
      181 gcgaaactca aatcaacgat caactgactg gtttcggtca gtaggaatat gaattcaaag
      241 gcaaccgtgc tgaatctcaa ggttcttcca aagataaaac ccgtcttgcc ttcgctggcc
      301 tgaaattcgg tgactacggc tccatcgatt atggccgtaa ctacggtgta gcatacgata
      361 tcggtgcatg gac
//
LOCUS       DAJTSA010000351          372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000351 DAJTSA010000000
VERSION     DAJTSA010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.353"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..311)
                     /locus_tag="OO369_004418"
     CDS             complement(<1..311)
                     /locus_tag="OO369_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5984393.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAJTSA010000352          357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000352 DAJTSA010000000
VERSION     DAJTSA010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.354"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(166..>357)
                     /locus_tag="OO369_004419"
     CDS             complement(166..>357)
                     /locus_tag="OO369_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR5984394.1"
                     /translation="TETVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGY
                     RLAEQVLMHEPLARMRWCG"
BASE COUNT           83 a          115 c           72 g           87 t
ORIGIN      
        1 aaagattaaa aaaacaccac aaaacggatg tttcttcaac accacttttg ctccatatga
       61 acggaaccga cgattaaact ggatggctct gattgattca gggtatgaat ggcggttttt
      121 tgctccgttt ccctcaaaat ggacgcaact tcccctctgc ggctctcagc cgcaccaccg
      181 catccgggcc agcggctcat gcatcaggac ctgctctgcc agacggtagc cccgcttcag
      241 ccccgtaaaa cgcatctgac tcccgcacag cacgcacttc agcgggtcaa ccttcagtaa
      301 cctctgatac atccctctcc aggtgatttg catcgccgtt tttctcactg tctccgt
//
LOCUS       DAJTSA010000353          353 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000353 DAJTSA010000000
VERSION     DAJTSA010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.355"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..264
                     /locus_tag="OO369_004420"
     CDS             <1..264
                     /locus_tag="OO369_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5984395.1"
                     /translation="FYRNDEFVFDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISD
                     EDKEKLKACRIYAKSLQAMDFSTITDKSSYNAIEWPASPEGSS"
     gene            288..>353
                     /locus_tag="OO369_004421"
     CDS             288..>353
                     /locus_tag="OO369_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5984396.1"
                     /translation="MSVVISGALTDGAGIPMSGYHI"
BASE COUNT           96 a           80 c           90 g           87 t
ORIGIN      
        1 ttttaccgca atgatgaatt tgtctttgac tacagactca aagcggaaga tgagcgtgat
       61 gcccttctgg ctcaggtcag tgcccggaca ggggaatggg aagaagacct gctgctgggg
      121 ttaatcagcg acgaagataa agaaaagctg aaagcctgtc gtatttacgc gaaatcgctg
      181 caggcgatgg atttcagcac catcactgat aaatcctcat acaacgccat tgaatggccc
      241 gcctctccgg aaggttcttc ctgatttaat ttatcgcgag aaaaacaatg tctgtagtga
      301 tatcaggtgc gctgactgat ggcgcaggta tccccatgtc cggataccat att
//
LOCUS       DAJTSA010000354          339 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000354 DAJTSA010000000
VERSION     DAJTSA010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.356"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            3..170
                     /gene="xisR"
                     /locus_tag="OO369_004422"
     CDS             3..170
                     /gene="xisR"
                     /locus_tag="OO369_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014884030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HCR5984397.1"
                     /translation="MLRTGLGARQIEAYRQNCWVEGFHFKRVSPLGKPDSKRGIIWYN
                     YPKINQFIKDS"
     gene            172..>339
                     /locus_tag="OO369_004423"
     CDS             172..>339
                     /locus_tag="OO369_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HCR5984398.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHQISS"
BASE COUNT          108 a           63 c           82 g           86 t
ORIGIN      
        1 tgatgcttcg cactggtctg ggtgccagac aaattgaagc ataccgccag aactgttggg
       61 tggaaggctt ccacttcaaa cgagtatctc ctttagggaa gccagacagt aagcgaggga
      121 ttatctggta caactatccg aagataaatc agtttatcaa agactcatga tatgtctaaa
      181 ttaccaacag gtgtcgagat tcgaggtaaa tacattcgca tctggttcat gtttcgagga
      241 aaacgatgtc gggaaacatt gaaaggctgg gagattacta acagtaacat taaaaaggcc
      301 gggaatttaa gagcgttgat agttcatcaa atcagttcc
//
LOCUS       DAJTSA010000355          335 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000355 DAJTSA010000000
VERSION     DAJTSA010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.357"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>335
                     /locus_tag="OO369_004424"
     CDS             <1..>335
                     /locus_tag="OO369_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5984399.1"
                     /translation="NGKVIAPACTLVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVP
                     IALEGCDVTVTKNATFTFSGTADGVQPTAFANQATTDAATNVALQMYLPDGSTSVTPG
                     TETSNIQLA"
BASE COUNT           92 a           79 c           78 g           86 t
ORIGIN      
        1 taatggtaaa gttattgctc cagcttgtac cctggtagct gcgacgaaag attcagtagt
       61 cacattgcca aatgtcagcg ccaccaagtt gcaaactaat ggagcagtct ctggcgttaa
      121 aactgatgta ccaattgcct tagaaggctg tgatgttact gtaactaaaa atgcgacgtt
      181 tacctttagt ggtacggctg acggcgtgca acctacagca tttgccaacc aggctaccac
      241 cgatgcagcg acgaatgttg ctctacaaat gtatctgcca gatggttcta catcggtaac
      301 tcccggtacc gaaacgtcga atattcagtt agcgg
//
LOCUS       DAJTSA010000356          334 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000356 DAJTSA010000000
VERSION     DAJTSA010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.358"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..143
                     /locus_tag="OO369_004425"
     CDS             <1..143
                     /locus_tag="OO369_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5984400.1"
                     /translation="AEVELATLTWVDWYNNRRLPERLGHIPPAEAEKAYYASIGNDDL
                     SA"
     gene            127..>334
                     /locus_tag="OO369_004426"
     CDS             127..>334
                     /locus_tag="OO369_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984401.1"
                     /translation="MICQPEFTDKTLSRKPGAVQKVNETDAEYLVRKFDELEAKCAAQ
                     ENKVIPVSTELPPANESVLLFDANG"
BASE COUNT          109 a           71 c           85 g           69 t
ORIGIN      
        1 gtgcagaagt ggaactggcc acactaacgt gggtggactg gtataacaat cgacgattgc
       61 cggaaaggct gggccacatc cctccggcag aagcagaaaa agcttattat gcttccatcg
      121 gaaacgatga tctgtcagcc tgagttcaca gataaaacac tctccaggaa acccggggcg
      181 gttcagaaag taaatgaaac agatgctgaa tacctggtgc gtaaattcga tgagcttgaa
      241 gctaaatgtg cagcacagga aaacaaagta ataccagtgt caactgaact gccaccagca
      301 aatgaaagtg ttttgttatt cgatgctaac ggag
//
LOCUS       DAJTSA010000357          333 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000357 DAJTSA010000000
VERSION     DAJTSA010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.359"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(43..330)
                     /locus_tag="OO369_004427"
     CDS             complement(43..330)
                     /locus_tag="OO369_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984402.1"
                     /translation="MDEEYRKDLQLWFGLTHASFCVMPRVFMEAMPPEWQEKMAQLLF
                     EYSDTIKTDVCGVHSCFVTAKDGNNRFMRMPEDILNYRHPRREFIESFLKK"
BASE COUNT           91 a           85 c           67 g           90 t
ORIGIN      
        1 taagccatta tcaaacccac ccgtagatag gctttgtaat ggctacttct tcagaaatga
       61 ttcgatgaat tcacgtcgag gatgacgata gttcagaata tcttctggca tcctcataaa
      121 gcggttgttg ccgtctttgg cagtaacaaa acagctgtga actccgcaga catccgtttt
      181 gatcgtgtcg ctatactcaa aaagcaactg agccatcttc tcttgccatt ctggaggcat
      241 agcctccatg aatactcgcg gcatcacgca gaacgacgca tgcgtaagac caaaccacag
      301 ttgcaggtct ttacgatatt cttcatccat cgt
//
LOCUS       DAJTSA010000358          332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000358 DAJTSA010000000
VERSION     DAJTSA010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.360"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            51..>332
                     /locus_tag="OO369_004428"
     CDS             51..>332
                     /locus_tag="OO369_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5984403.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPD"
BASE COUNT           83 a           70 c           98 g           81 t
ORIGIN      
        1 cgtccgaatg ttagccattc gagggttgtt aagtagtttc aggaggattt atggcgaaac
       61 cttttactcc cgaacagcgg gaagaactga agacgcgaat tgtggaactc gtgcatcagg
      121 acggtcgggt cacgattcgg cagttgtccg atgaaacagg tatcagtcgt gcgtctgtcg
      181 gtcgcttatg catagaactg gtcgcaagtg gtgatgtata taattctggc tacggcttat
      241 tcccgtctga acaggctcgc aaggactggc aaagcgcccg caaaaaactc tcgagagtaa
      301 aggtgaggaa accggttgtt gttgatccgg ac
//
LOCUS       DAJTSA010000359          327 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000359 DAJTSA010000000
VERSION     DAJTSA010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.361"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..327
                     /locus_tag="OO369_004429"
                     /pseudo
     CDS             1..327
                     /locus_tag="OO369_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           82 a           79 c          101 g           65 t
ORIGIN      
        1 acgtattacc actgtcagca acagcgacat catccggata aacgcagtgc ccgtgcgcag
       61 cgcgatgact ggctgaagaa agagatacag cgcgtatacg atgaaaatca caaggtatac
      121 ggtgtaaagt ctggcgtcag ttgttacggg aaggtatcag agtggccaga tgcactgtgg
      181 cacgtctcat ggcggttatg ggacttgccg gtgttctccg gggtaaaaag gtccgtacga
      241 ccatcagccg gaaagccgtt gccgcaggcg accgcgtaaa ccgtcagttc gtggcagaac
      301 gacctgacca gctgtgggtg gctgatt
//
LOCUS       DAJTSA010000360          325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000360 DAJTSA010000000
VERSION     DAJTSA010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.362"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            103..>325
                     /locus_tag="OO369_004430"
     CDS             103..>325
                     /locus_tag="OO369_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5984404.1"
                     /translation="MIYSNQLRYTGKDGVYFHLQGQPDNFLSLSATENMEFDSPMMVT
                     TQNMQSGQTVTDNVMRSPRTISISGVVVVG"
BASE COUNT          110 a           56 c           61 g           98 t
ORIGIN      
        1 aatttacgta gctcacgctt aacagagaaa atacttatat atcaaaggct tggttaaaaa
       61 tgattaaaat gcgctaagtc tttgaaatca aaagagaatt taatgattta ctctaatcag
      121 cttcgttaca ctggcaaaga tggtgtttac ttccatctac aaggccaacc agataatttc
      181 ttgtcactat cagcaactga aaacatggaa tttgatagcc ctatgatggt taccactcaa
      241 aatatgcaat cagggcagac tgtaacagat aatgtaatgc gttcacctag aactatttca
      301 atcagtggtg tggttgtggt tggtg
//
LOCUS       DAJTSA010000361          313 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000361 DAJTSA010000000
VERSION     DAJTSA010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.denovo.363"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT           82 a           70 c           74 g           87 t
ORIGIN      
        1 ttaaaaaatg gagcaatatt aggcaaacga tttgagatat gacctaaaag ctcggccaaa
       61 taataatgtc gtcgattatc gaagtcatca tgagtgattc tgtccccaca gtgcagcatc
      121 agcatgacag tgaattgtct ggcgtggtcc atttaatgag atggagatgg tccctctttc
      181 ctaaatagct gtgcctaata ccgctacact tttgccagcc catgtttgcc tccggggaat
      241 gggctgacgg ttccttcaaa atccattgca cgcagttatg tcggagcggg aacttgactc
      301 ctgtctttac ggg
//
LOCUS       DAJTSA010000362         6472 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.guided.164, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000362 DAJTSA010000000
VERSION     DAJTSA010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.guided.164"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..293
                     /locus_tag="OO369_004431"
     CDS             <1..293
                     /locus_tag="OO369_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
                     /protein_id="HCR5984405.1"
                     /translation="IELSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLK
                     HTLAFRDADGTTRLEYSDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            286..1020
                     /gene="frdB"
                     /locus_tag="OO369_004432"
     CDS             286..1020
                     /gene="frdB"
                     /locus_tag="OO369_004432"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5984406.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            1031..1426
                     /gene="frdC"
                     /locus_tag="OO369_004433"
     CDS             1031..1426
                     /gene="frdC"
                     /locus_tag="OO369_004433"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5984407.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            1437..1796
                     /gene="frdD"
                     /locus_tag="OO369_004434"
     CDS             1437..1796
                     /gene="frdD"
                     /locus_tag="OO369_004434"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5984408.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            1859..2992
                     /locus_tag="OO369_004435"
     CDS             1859..2992
                     /locus_tag="OO369_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5984409.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            3081..3614
                     /gene="blc"
                     /locus_tag="OO369_004436"
     CDS             3081..3614
                     /gene="blc"
                     /locus_tag="OO369_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5984410.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3611..3928)
                     /gene="sugE"
                     /locus_tag="OO369_004437"
     CDS             complement(3611..3928)
                     /gene="sugE"
                     /locus_tag="OO369_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5984411.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(4104..4250)
                     /gene="ecnB"
                     /locus_tag="OO369_004438"
     CDS             complement(4104..4250)
                     /gene="ecnB"
                     /locus_tag="OO369_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5984412.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(4361..4486)
                     /gene="ecnA"
                     /locus_tag="OO369_004439"
     CDS             complement(4361..4486)
                     /gene="ecnA"
                     /locus_tag="OO369_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5984413.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(4538..5104)
                     /gene="efp"
                     /locus_tag="OO369_004440"
     CDS             complement(4538..5104)
                     /gene="efp"
                     /locus_tag="OO369_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5984414.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            5146..6174
                     /gene="epmB"
                     /locus_tag="OO369_004441"
     CDS             5146..6174
                     /gene="epmB"
                     /locus_tag="OO369_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR5984415.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         1623 a         1654 c         1650 g         1545 t
ORIGIN      
        1 ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac
       61 gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg
      121 tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact cgcctggagt
      181 acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg
      241 atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc tgagatgaaa
      301 aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca
      361 ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg ctacatcaaa
      421 gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc
      481 tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac
      541 accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc
      601 gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc
      661 cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag
      721 ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac
      781 ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc
      841 gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt atggagctgt
      901 actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt
      961 cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa
     1021 ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg
     1081 gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt
     1141 gtggttcagc attgaactga ttttcgcgct gtttgccctg aaaaatggcc cggaagcctg
     1201 ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct
     1261 gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat
     1321 cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac
     1381 tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga
     1441 ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg
     1501 gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac
     1561 tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct
     1621 tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc
     1681 gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa tgggttttct
     1741 acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca atctaacgca
     1801 tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat
     1861 gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat ttgctgcccc
     1921 tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat
     1981 ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta
     2041 tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt
     2101 cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt
     2161 aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga atgggatcac
     2221 actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt
     2281 gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggctccagg
     2341 aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc
     2401 gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa
     2461 ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg gatatcgcga
     2521 aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc
     2581 gaccattgaa gatatggccc gctgggtgca aagcaattta cccccccttg atatcaatga
     2641 gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat
     2701 gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa
     2761 cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac
     2821 tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta
     2881 tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc
     2941 caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat
     3001 cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa
     3061 aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat
     3121 ttctggtcgt tgcctgtagt tttcctacgc cgccgcgtgg cgtgaccgta gtgaataatt
     3181 tcgacgccaa acgttatctc ggtacctggt atgagattgc ccgttttgat caccgttttg
     3241 aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc ggcctgaatg
     3301 tcattaataa aggctataac cctgacagag gaatgtggca gcagagtgaa gggaaagcgt
     3361 actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt cctttctatg
     3421 gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt tgcggcccgg
     3481 accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg
     3541 agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc
     3601 agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca
     3661 gactcgccag acgcatcggg ttagcggact caccgagcag cacaatgccg gtgatggccg
     3721 cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt aacgatttca
     3781 tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacggtaata acactcggcg
     3841 tcaaacggct aaagccgtgg gtatatttca ggccaacggc ccataccact tccagcagac
     3901 cagcaataac taagataatc caggacatat caggctccgg aaatttgggg ccgtccccgg
     3961 tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat attttgccag
     4021 gtggcagggg aatttcaact attaccttta tgacgatcgc agaaaaaaca aacggcacga
     4081 cactgctgtc gtaccgttat tgcttattgc tgcgctttcg ttgctgcgcc agaaatcgcg
     4141 ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaggc agttaatact
     4201 gttgaaagca ccagaacaga aaagatcgct gcaattgtct tcttcaccat aacgtcttcc
     4261 ttttaggtaa tgttatttat gtttgcctat agcaaattaa taatagacaa catcgccaaa
     4321 aatgatggaa atctgatgat ttttcggaag agaagaaaaa ttagctggca gcgcgagaga
     4381 tggagttacc gagatgtttg atgtcttcgc cgaaaccgca agcggtgtta cagcccgtga
     4441 gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt catcatctct gacctgaata
     4501 agtgatggtg cagcctgcag gccgcaccac aaccgcatta cttcacgcga gagacgtatt
     4561 caccagagcg ggtatccact ttgatgactt cgccgatttg tacgaacagc ggaactttaa
     4621 ccacagcgcc agtagacagg gtagccggtt tgccgccagt acctgcggta tcacctttca
     4681 ggcccggatc ggtatcaacg atttccagtt caacgaagtt cggcggagta acggagatcg
     4741 gctgaccatt ccacagagtt acgatacact ctgcctgatc cagcagccat ttagcattgt
     4801 cgccaattgc ttttgcatca gcagacagct gctcgaaagt ttcgttgttc atgaagtgcc
     4861 agaactcacc gtcgttgtac aggtaagtca ggttcatatc gacaacatca gcgccttcag
     4921 cggaatcagt agatttgaag gttttttcta cgcgagtacc ggtcagcaga cgacgcagtt
     4981 taacgcgagc aaatgcctgg cctttacccg gttttacgaa ttcactcgct tcaaccgcgt
     5041 aaggttcgcc gtctaacatg attttaagac cagcacgaaa atcgttgcta tagtacgttg
     5101 ccataaggcc ctctgaaatt tgttaattgg tagctaagcc acaaaatggc gcatattgta
     5161 accctaaata ccccatccag agaagattgg ttaacgcaac ttgccgatgt tgtgaccgat
     5221 cctgatgaac ttctgcgtct tttgaatata gacgcggacg aaaaactgtt ggccggacgc
     5281 agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg catggagaaa
     5341 ggcaatccgg acgatcctct tttgcgtcag gtacttacct cgcaagatga gtttgtcgtc
     5401 gcgtccggat tctccaccga cccactggaa gaacagcaca gcgtagtgcc tggtttgttg
     5461 cataaatacc acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt aaattgccgc
     5521 tattgcttcc gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg taactggcag
     5581 actgcacttg agtatgttgc tgcgcatccg gaactggacg agatgatttt ctccggcggc
     5641 gatccgctga tggcgaaaga gcacgagctg gactggttgc tcacacaact ggaagccatc
     5701 ccgcatatca aacgtctgcg gattcacagc cgtctgccga ttgtgatccc ggcacgtatc
     5761 accgaggcgc tggttgaacg ctttgcccgt tctacgctgc aaatcttgct ggtgaatcac
     5821 atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg cgatggctaa attgcgccgt
     5881 gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg gtgtgaacga taacgcacaa
     5941 acgttggcaa acctgagtaa tgcgttgttc gatgccggcg tgatgccgta ttacctgcat
     6001 gtgctagata aagtacaggg cgcggcgcat tttatggtga gtgatgacga agcacggcag
     6061 attatgcgtg agttgctgac attggtgtcg ggatatctgg tgccgaaact ggcgcgagaa
     6121 attggcggcg aacccagcaa aacgccgctg gatctccagc tacgccagca gtaactgatt
     6181 aaacagggaa gttaaacatt aaaattgcct gatgcgctac gcttatcagg cctacatcgt
     6241 gcattgaaat tgaatggatt tgcaatgaac tgtaggccgg ataaggcgtt tacgccgcat
     6301 ccggcaagag caacaaacgc aagtacagca accactgaga aatttaatct ttgcacgttg
     6361 cataaccttt aattattcat atgcattgct gttgttactt tttatatagc taacaccaat
     6421 aattaacctt tcttttcggt aatgactcca acttactgat agtgttttat gt
//
LOCUS       DAJTSA010000363         3070 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate Stool culture
            SAMEA110466914-rid15818553.guided.215, whole genome shotgun
            sequence.
ACCESSION   DAJTSA010000363 DAJTSA010000000
VERSION     DAJTSA010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466914
            Sequence Read Archive: ERR10074780
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477446.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477446.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:20:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,444
            CDSs (total)                      :: 4,372
            Genes (coding)                    :: 3,983
            CDSs (with protein)               :: 3,983
            Genes (RNA)                       :: 72
            rRNAs                             :: 2, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 62
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 181 of 389
            Pseudo Genes (incomplete)         :: 105 of 389
            Pseudo Genes (internal stop)      :: 147 of 389
            Pseudo Genes (multiple problems)  :: 43 of 389
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466914-rid15818553.guided.215"
                     /serovar="2A"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="South Africa"
                     /collection_date="2012-02-27"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1214)
                     /gene="pepD"
                     /locus_tag="OO369_004442"
     CDS             complement(<1..1214)
                     /gene="pepD"
                     /locus_tag="OO369_004442"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HCR5984416.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANS"
     gene            1476..1934
                     /gene="gpt"
                     /locus_tag="OO369_004443"
     CDS             1476..1934
                     /gene="gpt"
                     /locus_tag="OO369_004443"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5984417.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2026..>3070
                     /gene="frsA"
                     /locus_tag="OO369_004444"
     CDS             2026..>3070
                     /gene="frsA"
                     /locus_tag="OO369_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5984418.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQG"
BASE COUNT          707 a          795 c          800 g          768 t
ORIGIN      
        1 gaattagcgt ccggctgcca gccaggatat gcgcctttcg cttcggtttt cgcgccagcc
       61 agtttaccca gcgaatccag catgctcacc acgtagtctt taccgctgtc gattagtgaa
      121 cggatcaggc agtgaatttc tacgttattg tcagtcatgg tcaccacacc gacgttcagg
      181 gaggtttcga ccacaccttt tgccacgtcg gaattgcgga tcacaccgtt cggggtggcg
      241 ttcagcagac gaataaaggt atcgcgagat ttcgcaatca gggcagcttt atcgttcgct
      301 acagagtcca gcaacaaggc cagatttttc tctttctctg ccagctcgtt tttcaggatc
      361 tcctgatagg tattcaccag agatttcaga gcgtcgactt tatcagctgc gacagcaatg
      421 gtcgcaaagg cttcacgcgg gatggcgtta cgcagtgtgc cgccgttgaa atcgataagg
      481 cgcaggtcca gttcttctgc atgacccgcc aggaagcgca ccagcagttt gttggcatta
      541 cccaggccaa cgtggatttc accgccggag tgaccgcctt tcagaccttt taaggttaac
      601 ttgaaggttt caaaaccagc tggaaccgct tcacgatcta aatgcaggtt ggaggtgaag
      661 tcgatacccc ccgcacaacc catgtagatt tcaccttctt cttcggagtc ggtgttaatc
      721 agaatatcag cctgcaacca gttgctctgt aagccgaacg caccgtccat accggcttct
      781 tcggtcatgg tcagcagcac ttccagcggg ccgtgaacca cgttttcgtc agccagaacc
      841 gccagtgcag aggccatacc aatgccgtta tccgcaccca gcgtggtgcc gcgcgcttta
      901 acccattcgc catcaatata aggctggata ggatctttcg tgaagtcatg cacggtgtcg
      961 ttatttttct gcggcaccat atcgaggtgg gcctgtaaga cgaccggttt acgattttcc
     1021 atacctgcgg tagcaggttg acgaatcagg atattaccta cctgatcgcg ttcgacatgg
     1081 aaacctttct cttttgccca accaacaatg tattcagcga gttgctcttc atgataggac
     1141 gggtgaggaa tagaacagat tttggcaaaa atatcccaca gcggctgtgg agataattga
     1201 gacagttcag acacgttaag tctccttgtc gatcacccgc aaaacagtat tgcaggtcac
     1261 agggttagca gaaaatgttg tcaacacaag acaggcttgc gagatatgta ttgagaatac
     1321 cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac
     1381 tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt
     1441 tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct
     1501 gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat
     1561 ggaaaggcat tattgccgta agccgtggcg gtctggtacc gggggcgtta ctggcgcgtg
     1621 aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc
     1681 gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg
     1741 acctggtgga taccggtggt actgctgttg cgattcgtga aatgtatcca aaagcgcact
     1801 ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata
     1861 tcccgcaaga tacctggatc gaacagccgt gggatatggg cgtcgtattc gtcccgccaa
     1921 tctccggtcg ctaatctttt caacgcctgg cattgccggg cgttgttctt tttaacttca
     1981 ggcaggttac aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac
     2041 ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc
     2101 cgctttaatc acggcgcaca accgcctgtg cagtcggctc ttgatggtaa aaccatccct
     2161 cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggtattga cccacgtgaa
     2221 atcctcgacg tccaggcacg tattgtgatg agcgatgctg aacgtaccga cgatgattta
     2281 tacgataccg tgattggcta ccgtggtggc aactggattt atgagtgggc cacccaggcg
     2341 atggtgtggc aacaaaaagc ctgtacggaa gaagatccgc aactcagtgg tcgacactgg
     2401 ctgcatgcgg ctacgttgta caacattgcc gcctatcctc atctgaaagg agatgacctg
     2461 gccgagcaag cgcaggcttt atcaaaccgc gcctatgaag aggccgccca gcgtctgccg
     2521 ggcacgatgc ggcagatgga gtttaccgta cccggcggtg cgcccatcac cggctttttg
     2581 catatgccga aaggcgatgg cccgttcccg acagtattaa tgtgtggtgg tctggatgcg
     2641 atgcagacgg actattacag cctgtatgaa cgttattttg cgccgcgcgg cattgcgatg
     2701 ctgactattg atatgccgtc ggtgggcttt tcttcaaaat ggaagctcac ccaggactcc
     2761 agcctgttgc atcagcacgt cttaaaggcg ctgcctaacg taccgtgggt ggatcacact
     2821 cgcgtcgcgg cctttggttt ccgtttcggc gctaacgttg ccgtgcgtct ggcatacctt
     2881 gaatcgccgc gtctgaaagc ggttgcctgt cttggtccgg tagttcatac cctgttgagt
     2941 gattttaagt gccagcaaca ggtgccggaa atgtatcttg acgttctggc gagtcgtttg
     3001 gggatgcatg atgcttccga cgaagcgtta cgcgtggagc tgaatcgcta ttcattaaaa
     3061 gtgcaaggat
//
